# MOTE MARINE LABORATORY

... established 1955

1600 CITY ISLAND PARK SARASOTA, FLORIDA 33577 

### E TELEPHONE 

### 813/388-4441 1David

Submitted to:

Submitted by: 

### FINE MESH 

### SCREEN SURVIVABILITY STUDY B16 BEND UNIT 4 

### TAMPA ELECTRIC COMPANY

A Technical Report by Bruzek and Selvakumaran Mahadevan
Tampa Electric Company Post Office 

### Box 111 Tampa, 

### F1 orida 33601
Mote Marine Laboratory 1600 City Island Park Sarasota, F1 orida 33577 (813) 388-4441
December, 1986

### TABLE OF 

### CONTENTS 

### Paqe 1.0 

### Introduction 1 

### 2.0 

### Study Rationale 1 

### 2.1 Background 1 

### 2.2 Station Locations 4 3.0 Methods 4 3.1 Laboratory 

### Field Site 4 3.2 Representative Important 

### Species 6 

### 3.3 Sampling Procedures for 

### FMS Survivability Studies 6 

### 3.3.1 Screenwash Sampling 8 

### 3.3.2 Organism 

### Return Discharge (ORD) Sampling 8 

### 3.3.3 Control Sampling 9 

### 3.4 Laboratory 

### Procedures for FMS Survivability Studies 9 

### 3.4.1 Latent 

### Survival Tests 9 

### 3.4.2 Initial Survival Tests 10 

### 3.4.3 Sampl 

### e Identification 10 

### 3.5 Data Management and 

### Analysis 11 

### 4.0 

### Results 11 

### 4.1 Screenwash 11 4.2 

### Organism Return Discharge 17 4.3 

### Control 22 4.4 

### Survivability Comparisons between Sites 27 4.5 

### Survivability and 

### Abiotic Parameters 27 

### 4.6 Survivability Comparisons to 

### Prototype Study 30 5.0 

### Discussion 30 5.1 

### Overall Survival 30 5.2 

### Effectiveness of FMS 

### 33 5.3 

### Comparison to Prototype 33 6.0 

### Summary and 

### Conclusions 35 

### 7.0 

### Literature Cited 37 APPENDICES: FMS 

### SURVIVABILITY STUDIES 

### DATA Appendix A 

### - Screenwash Station 

### Tables A1 

## - A42 bppendix 

### B - Organism 

### Return Discharge Station Tables B1 

### - B42 Appendix 

### C - 

### Control Station Tables C1 

### - C421.0 INTRODUCTION Tampa Electric Company is presently operating four coal-fired electric generating 

### units at Big Bend Power Station. The site is located on the eastern shore of Tampa Bay in North Ruskin, Florida (Figure 1). All four units utilize once-through cooling systems; each 

### unit circulates approximately 537cfs of water. The four units draw water from a single intake canal and release heated water to the Bay via 

### a single discharge canal. To reduce entrainment at the plant, Units 3 and 4 were fitted with fine mesh screens (0.5mm); screened organisms are returned to the Apollo Beach 

### northern embayment (Figure 2). In fulfillment of the Clean Water Act, an NPDES permit was obtained by Tampa Electric Company 

### to construct and operate Unit 4. A Biological Monitoring Program was conducted by Tampa Electric Company in accordance with conditions of the NPDES Permit (No. FL 0000817) and the State of Florida Power Plant Site Certification (Application No. P.A. 79-12; Case No. 80-1723). The general requirements of the program are summarized in the Final Environmental Impact Statement for Tampa Electric Company, Big Bend Unit 4 (pages 4-64 through 4-66). This report summarizes the results of the Fine Mesh Screen Survivability Study, which was conducted during the spawning season of March through September, 1985, after Unit 4 began commercial operation (February 25, 1985).
2.0 STUDY RATIONALE

- . 2.1. Backsround Prior 

### to the addition of Unit 4 

### at 

### the Big Bend Station, concern was expressed for organism losses due to the operation of the Unit 4 cooling water intake. An initial evaluation by Tampa Electric Company indicated the potential for effective application of fino mesh screening (FMS) to reduce entrainment levels at Big Bend. Preliminary studies were conducted at the site in the summer of 1979 

### to determine whether available Representative Important Species (RIS) were capable of surviving impingement on a fine mesh screen under existing screen design conditions and to determine the relationship between specific

1

# YAREA OF STUDY

LSE~;;OUGH~

BIG BEND


### ST. PETERSBURG 

# h

TAMPA BAY /-J



# Po 5 Nautical ’ I Miles



### .. .igure 1. Site Location of the Big Bend Power Station.

# f

0 3000 1 I J SCALE- FEET Figure 2. Location of Fine Mesh Screen Sampling Stations.

...

### a velocity/impingement duration and survival rate. The results of the 1979 studies are 

### presented in Appendix 3 of 

### the August 1980 316 Demonstration 

### (TEC: 1980a).Additional detailed investigations were conducted in 1980 to test 

### the biological and engineering feasibility of FMS utilizing a prototype traveling screen, including all features of an in-service installation. Biological investigations 

### were conducted from March through August 1980. Preliminary results of testing through mid April 

### were presented in Appendix 4 of the 316 Demonstration (TEC, 1980a) Final results were summarized in the FMS report (TEC, 1980b). 2.2. Station Locat ons
- The survival studies 

### were conducted to evaluate the 

### effectiveness of fine mesh screens at the Big Bend Station. Sampling for the survival study was conducted at three locations pertaining to Unit 4. The screenwash station was in front of the screens after the organisms 

### were screened and washed to the screen return; the organism 

### return discharge (ORD) station was at the discharge point of the screened organisms in the northern embayment of Apollo Beach; and the control station was at the intake canal in front of the intake 

### screens. Station locations are shown in Figure 2.
3.0 METHODS Methods used during this study 

### were essentially si'milar to those used during the FMS Prototype study conducted in 1980 (TEC, 1980b). 3.1. Laboratory Field Site An on-site facility was established at the Big Bend station for 

### the fine mesh screen survivability studies. This facility consisted of a 

### test trailer with a flow-through seawater system, an adjacent office trailer, and a sample wash and storage trailer. The on-site facility was located along the intake canal near the fine mesh screens (Figure 3). All live meroplankton sortina and holding of organisms for 48 hours was conducted at this facility.

4OFFICE TRAILER

I L--. I--- 

## - I I I I 1 I I L-, 

### - I L,,

TRAILER



# I
Figure 3. Site Plan for Laboratory Field StationThe test facility was a 60 foot 

### x 12 foot trailer capable of holding 1500 containers (500ml each) in situ (intake canal) 

### on water tables equipped with once-through seawater. The trailer 

### also had the capability for eight persons 

### to 1ive-sort meroplankton. The flow-through seawater system consisted of 

### two, 1/2 hp pumps which pumped intake canal water through 4 sand filters up 

### to a 300 gal head 

### tank. The filtered seawater was then gravity fed from the head tank 

### to thc holding facility. The adjacent office trailer had desk space for 8 persons, equipment for vial preservation and transport, and 

### a net and equipment storage area. The sample wash trailer was used 

### to rinse samples and equipment and for the storage of retained samples. 3.2. Representative Important Species The survival studies were designed 

### to concentrate on certain target species which were found in high abundance in the 1980 studies 

### (TEC, 1980b). These Representative Important Species (RIS) were: h- 

# ""-7- Anchoa mitchilli 

### - eggs, prolarvae and postlarvae; i" SG+W Harenqul 

### a jaguana 

### - eggs, pro1arva2 and pc;stl arvae; k-2 Sciaenidae
- eggs, prclarvae and postlarvae; +,.& caa--b Meni.ppe mercenari a 

## - zoea and mega1 ops ; y~d-c-I,ny Upogebia sp. 

### - mysis and postlarvae. In addition, eggs and larvae of other fish species which appeared in large numbers; and any juveniles which were collected were held in latent mortality experiments. Because of the difficulty of distinguishing larvae of Menippe and llpogebia during live sorting, zoea and larvae of 

### a number of crustacean species were held in latent mortality tests. 3.3. Sampling Procedures for FMS Survivability Studies Fine mesh screen (FMS) field studies commenced the first week of March 1985. Field studies were conducted each week from March 5, 1985 until October 1, 1985, for a total of 31 weeks of sampling. (See Table 1 for actual dates.) Two collections per day and 

### two per night were taken at the screenwash

6Table 

### 1. Sampling dates for the 

### FMS Survivability Study.

Survival Studies
March 

### 5, 1985 

### March 11, 1985 March 18, 1985 March 25, 1985 April 2, 1985 April 8, 1985 

### April 16, 1985 April 22, 1985 

### April 29, 1985 May 6, 1985 May 13, 1985 May 20, 1985 May 28, 1985 

### June 3, 1985 

### June 10, 1985 

### June 18, 1985 Survival Studies
June 25, 1985 July 1, 1985 July 9, 1985 July 16, 

### 1985 July 23, 1985 July 30, 1985 August 6, 1985 

### August 14, 1985 August 20, 1985 

### August 27, 1985 September 

### 4, 1985 September 10, 1985 September 17, 1985 September 23, 1985 October 1, 198~

7c

1
I 

# 1

-.

I

x 

# e



# f

P 

### and ORD stations one day each week. One collection per day and one per night were taken from the control station one day each week.
3.3.1. 

### Screenwash Sampling The low and high pressure screenwash of the fine mesh screens emptied into a common trough which flowed to a sump at the beginning of the orgmjsz return system. The screenwash station was sampled by suspending a lm mouth diameter, 505 

### micron mesh net in front 

### of the trough where it emptied into the sump basin at the beginning of the organism return system. A bucket attached to the cod end of 

### the net was used to collect organisms retained by the net. A 

### PVC frame inside the net held it open, reducing damage to the organisms from friction against the net. The net was fished for 3 

### minutes, and a11 organisms retained were carefully rinsed down into the bucket. Abiotjc measurements including temperature, dissolved oxygen, salinity, and conductivity were taken in the sump prior to net sampling. The status of the screens, including the number of screens, screenwash pumps and condenser cooling pumps operating, as well as whether the bypass gates were open or closed was recorded during each sampling. The sample was then immediately returned to the test trailer for sorting

# . 3.3.2. 

### Organism Return Discharqe (ORD) Sampling The 

### ORD pipes exit at the end of 

### a walkway which extends 500 

### feet along a 

### sheet pile wall in the Apollo Beach northern embayment. The ORD station was sampled using a specially constructed 505 

### micron mesh net supported by 

### a floating rectangular frame (2.5 

### ft 

### x 4 

### ft). The net was positioned under the flow from the discharge pipe, and organisms retained were carefully rinsed down to a bucket attached to the cod end of the net. The net was fished for 3 

### minutes. Abiotic measurements including temperature, dissolved oxygen, salinity, and conductivity were taken in the discharge area prior to net sampling. The sample was returned to the holding trailer immediately after collection.3.3.3. Control Sampl i

### ng The control sample was 

### taken by lowering 

### a standard 1 meter 

### mouth diameter, 505 micron mesh net from 

### a walkway bridge 

### over the intake canal. Enough line was released 

### to allow the net 

### to sink 

### to mid depth, where it was allowed 

### to fish for 15 minutes. The continual flow of the intake canal was suffic.ient enough 

### to allow for adequate sampling 

### from the stationary net. Organisms retained 'by the 

### net were carefully rinsed 

### to the cod end 

### of the net, and the contents were then carefully poured into 

### a bucket of ambient intake water. Abiotic measurements, including temperature, dissolved oxygen, salinity, and conductivity, were taken in the intake canal prior 

### to net sampling. The sample 

### was returned to the holding trailer immediately after collection. 3.4 Laboratory Procedures 

### For FMS Survivability Studies
3.4.1. Latent Survival Tests The latent survival tests were conducted 

### to examine the delayed survival response of the target species and other abundant species impinged 

### on the screens. Samples obtained from the screenwash, ORD, 

### or control stations were brought 

### to the test trailer, where 

### a subsample was taken by the sorting supervisor. The subsample was then sorted using glass trays 

### on a lighted table equipped with flow-through water. The sorted organisms were placed in individually marked 

### jars (5 

### to a 

### jar); the 

### jar number and contents were recorded and checked by the holding supervisor. The jars were .-then placed on the appropriate water table for the latent survival tests. 

### For each sample, up 

### to 30 test.organisms of each lifestage of the species were held, where possible. Crab zoea were held in individual compartments of plastic tackle boxes 

### (to avoid cannibalism). Once the organisms for holding were drawn off the first subsample, the rest of the RIS were sorted as live 

### or dead and preserved in separate vials (initial survival). If a total of 30 organisms for each species and lifestage was 

### not available from the first subsample, a second subsample was drawn and sorted only for the live holding organisms necessary. The procedure

9

# 1 

# 1

- 7. I F 1 was repeated once more, if necessary. A maximum of 3 subsamples per sample were processed. Once the organisms for holding and initial survival were removed 

### from the sample, the remains were preserved and stored in labelled 

### jars. Latent survival observations were performed 

### at 6, 12, 24, 36, and 48 hours after the collection time. The number of remaining live and dead organisms in each test container was recorded' 

### on the data sheet. Observations such 

### as hatching of eggs 

### or missing organisms were also recorded. Dead organisms were removed from the 

### jars, placed in labeled vials, preserved in formalin, and stored in transport trays for later transfer 

### to Mote Marine Laboratory (MML). At the end of the 48 hour period, all remaining live org,anisms were placed in labeled jars, preserved, and taken 

### to MML. 3.4.2. Initial Survival Tests Initial survival tests were conducted to examine the survival of 

### the organisms immediately after the collection of samples from the screenwash, -organism return canal, and control stations. The first subsample was sorted 

### to

obtain the necessary numbers of organisms for the latent survival tests; the remainder of the subsample was then sorted into live and dead RIS organisms for estimating initial survival. The sorted organisms were placed into separate labeled vials for live 

### or dead, then preserved in formalin and stored for later identification at MML. The sorting process was limited 

### to 

### a maximum of 

### two hours after sample collection in order 

### to minimize handling mortality. Initial survival observations were performed 

### on the lifestages of the RIS species only.

-. 3.4.3. Sample Identification The vials of preserved organisms were placed in 

### a transport rack 

### at the field site for transfer 

### to MML. A custody sheet with the vial number and position of vials in the rack accompanied it during transport, 

### to avoid potential loss of data during sample transfer. Organisms were sorted and identified 

### at MML. Identifications were made through the use of standard literature sources and MML reference collection. The sample number, species name, lifestage and number were recorded on bench

10i

1 I 

### 1 

## i sheets for later computer entry. A voucher 

### reference collection of 

## f i taxonomically confirmed 

### species was maintained. 

# 3 3.5. Data Management and Analysis 

### i Computer files 

### were created with data entered directly from benchsheets 

### or logbooks and using a dBASE 

### I1 database program. Printouts 

### were checked and corrections made to e'nsure the accuracy of the databases. Manipulation and analysis of data 

### was then performed using standard computer packages on an IBM compatible Zenith 100 system. 

# 1 1 1 4.0 RESULTS Initial and latent Survivability of selected taxonomic groups 

### were analyzed for the screenwash, organism return discharge, and control stations. B 

### t Results are reported for day and night samplings per month, as well as overall
ii mean values. Abiotic data collected in conjunction with the 

### FMS survivability 

## $ tests are tabulated and presented in Table 2. 1 4.1. Screenwash Stations 

# i I Mean initial survivability of selected taxonomic groups collected at the 

### screen 

### wash station 

### is presented in Table 3. Initial survivability and percent 

# ! hatchability of selected eggs collected from day and night samplings of March through September 1985 are given in Appendix A, Tables 

### A-1 through A-7. 

# 1 Similarly, initial survivability of selected fish larvae i's provided in Appendix 

### A, Tables A-8 through A-14. The initial survivability of selected invertebrates 

# 7 collected from day and night samplings March through September is given in Appendix 

### A, Tables A-15 to A-21. 

# i Of the eggs collected from the screenwash station samplings, only two taxonomic groups, the Engraulids and 

### the Sciaenids, were collected in sufficient numbers for meaningful analysis (Table 3). Engraulids eggs bad an overall 

### 1 initial survivability of 48%, and wet-e collected from all months with the greatest numbers and survival 

### seen duriq the day samplings. Sciaenid eggs f



### - 11

### Table 2. 

### Mean values of 

### abiotic measurements taken during the 

### FMS Survivability study.

SCREENWASH March April May June July August September Temperature 22.6 25.2 28.2 30.9 30.6 30.8 28.5 

### (OC) Dissolved 8.0 7.7 6.8 6.7 6.8 6.1 6.8



### Oxygen (mg/l) Salinity (ppt) 38.6 29.5 30.7 33.3 30.9 27.7 25.5 

### Conductivity 42.4 45.3 50.1 52.8 51.6 44.6 41.1 (

### mmhos) 

### ORGANISM RETURN DISCHARGE Temperature 24.2 27.0 31.1 32.5 32.3 32.6 31.5 

### (OC) Dissolved 7.5 6.8 6.5 5.4 5.8 5.6 6.1



### Oxygen (mg/ll Salinity (ppt) 28.9 29.9 30.8 31.5 31.O 29.3 25.5 

### Conductivity 44.1 47.4 53.0 54.7 52.8 $8.9 43.9 

### (mhos) 

### CONTROL Temperature 22.2 24.7 28.1 31.2 30.4 30.5 28.7 

### (OC) Di 

### ssolved 8.3 7.6 7.4 7.3 6.7 6.5 9.5 

### Oxygen (ms/l) Salinity (ppt) 28.4 29.5 30.3 31.1 30.6 28.5 24.5 

### Conductivity 40.7 44.4 50.0 52.7 50.5 46.5 36.8 

### (mmhos)

### --

--

-- --

--

-- 

### --

--

-- 

### Table 3. 

### Summary data of 

### initial survivability tests at Screenwash Station, Big Bend Power Station (1985).



### FISH 

# - EGGS :



### CLUPE

### IDAE ENGRAULIDAE SCIAENJDAE SOLE

### IDAE LARVAE:

CLUPEIDAE ENGRAULIDAE ATHERINIDAE SPAR1DAE Lagodon rhomboides SCIAENIDAE Bai rdiel 1

### a chrysoura Poqonias cromis Cynosci on nebulosus 

### Cynosci on arenarius Leiostomus xanthurus BLENNIIDAE GOBI IDAE SOLEIDAE INVERTEBRATES ZOEA:

PENAEIDAE CARIDEA PORTUNIDAE PAGURIDAE XANTHIIDAE PINNOTHERIDAE GRAPS

### IDAE MEGALOPS :



### XANTH

### II

### DAE GRAPSIDAE INITIAL SURVIVABILITY Total # 

### Organisms Tested Mean S.D.

219 0.78 0.04 21,899 0.48 0.36 51 202 0.63 0.03 29 0.88 0.18

2 0.50 0.71 1,651 0.16 0.04 1 0.00 0.00 1 0.00 0.00 1,632 0.61 0.33 2 0.00 0.00 1 0.00 0.00 25 0.00 0.00

3 0.25 0.35 107 0.10 0.00

35 0.00 0.00

' 6 1 

### .oo 0.00 l 

### ,057 c.72 0.01 45 0.94 0.07 83 0.56 0.04 11 143 0.93 0.04 33,287 0.89

. 0.06 512 0.92 0.07

25 0.77 0.33 149 0.82 0.20

13

### exhibited an overall initial survivability of 63%, 

### and were collected from all months with the highest numbers being collected at night. The hatchability of 

### eggs that were held 

### in the latent mortality tests is an index of 

### the viability of 

### the 

### live eggs that initially survived. Hatchability data is summarized in Table 33. 

### Both the Engraulid and Sciaenid eggs had average hatchability of 

### greater than 70%. Of 

### the larvae collected for initial survivability testing, only Engraulid and Sciaenid larvae were collected 

### in sufficient numbers for meaningful analysis. Engraulid larvae had an overall mean initial survivability of 16%, 

### while Sciaenid larvae exhibited 61% 

### (Table 3). 

### Both showed 

### 1 ittle difference between day and night samplings. Invertebrates had the highest overall initial survival, exceeding that of fish larvae (Table 3). 

### Caridean zoea had initial survivability of 

### 72%, 

### with no difference between day and night sampling. Xanthiidae zoea had 93% 

### initial survivability, with larger numbers 'collected during the day but no difference in day/night survival. Pinnotheridae zoea had initial survivability of 89%, 

### with larger numbers tested from day sampling but no differences 

### in survival. Grapsidae zoea had 92% 

### initial survivability; most individuals were collected during the day, but no differences in survival between day and night were exhibited. Megalops larvae were not collected in sufficient numbers for meaningful analysis. Latent survival was examined at 24 

### and 48 

### hrs and data is summarized in Tables 4 

### and 5. 

### Since egg development time of 

### selected taxonomic groups tested was generally less than 48 

### hrs, eggs were tested only for-initial survivability and hatchability. The 24 

### hr and 48 

### hr latent survivability of selected hatched fish larvae collected from day and night samplings .at the screenwash for March through September 1985 

### is given in Appendix A, 

### Tables 

### A-22 to 

### A-28, the 24 

### hr and 48 

### hr 

### 1atent survivabil i 

### ty of selected fish 

### 1 arvae collected from sampl ings at the screenwash March through September is given in Appendix 

### A, Tables A-29 to A-35, and the latent survivability of 

### select invertebrates collected during this period 

### is presented in Appendix A, Tables A-36 through A-42. 

### Of the larvae which were collected in sufficient numbers, the Engraulids

14

### -- --



### -- -- --

-- --

-- 

### --

-- Table 4. 

### Summary data of 

### latent survivability tests for 24 

### and 48 

### hours, Screenwash Station, Big Bend Power Station (1985). 

### LATENT SURVIVABILITY Total # 

### Organisms 24 

### hrs 48 

### hrs Tested Mean S.D. 

### Mean S.D. FISH 

## - EGGS:

CLUPEJDAE ENGRAULIDAE SCIAENIDAE SOLEIDAE LARVAE:

CLUPEIDAE ENGRAULIDAE ATHERINIDAE SPAR

### IDAE Laqodon rhomboides SCIAENIDAE Bai rdiella chrysoura Pogonias cromi s 

### Cynosci

### on nebul osus 

### Cynoscion arenarius Leiostomus xanthurus
I 

### BLENNIIDAE GOB

### I IDAE SOLEIDAE INVERTEBRATES ZOEA:

PENP.E

### IDAE CAR1DEA PORTUNIDAE

L 

### PAGURIDAE XANTHIIDAE PINNOTHERIDAE i 

### GRAPSIDAE : 

# i MEGALOPS : 

### XANTHIIDAE GRAPSIDAE JUVENILES:

Lo1 

### 1 i

### quncul a 

### brevi s 4 0.25 0.35 322 0.45 0.13 549 0.44 0.04 8 0.30 0.18
28 0.59 0.12 0.44 0.16 2,200 0.77 0.10 0.68 0.04 1 0.00 0.00 0.00 0.00 1 0.00 0.00 0.00 0.00 1,852 0.73 0.02 0.63 0.04 6 0.50 0.50 0.50 0.50 7 1-00 0.00 1.00 0.00 2 0.50 0.71 0.50 0.71 143 0.57 0.04 0.44 0.13

15 0.62 0.28 0.62 0.28 39 0.39 0.55 0.36 0.32 941 0.98 0.03 0.96 0.04

52 0.95 0.07 0.95 0.07 1,992 0.78 0.01 0.07 0.06 34 0.96 0.06 0.81 0.03 97 0.77 0.07 0.76 0.09 1,771 0.88 0.02 0.80 0.06 3,499 0.85 0.00 0.71-

. 0.04 176 0.91 0.07 0.85 0.03
215 0.99 0.02 .0.97 0.04 1,230 0.98 0.01 0.95 0.00
44 0.03 0.01 0.02 0.03

15

### --
--

-- --
--

-- -- 

## --

-- 

### --
--

-- 

### --
--

-- Table 5.

Taxa Clupeidae Anchoa mitchilli Atherinidae Sparidae Laaodon rhomboides Sciaenidae Bairdiella chrysoura Poqonias cromis Cynoscion nebulosus Cvnoscion arenarius Leiostomus xanthurus

. 

### Bl enniidae Gobi idae Soleidae Summary of the latent survival of fish larvae from Big Bend Screenwash Station for 24 and 48 hours, for all samplings. (1.00 = 100% survival).
24 

### hrs 48 hrs No. Tested Mean S.D. 

### Mean S.D.
5 0.50 0.00 0.50 0.00 105 0.75 0.40 0.73 0.39

3 0.66 0.57 0.66 0.57 275 0.80 0.31 0.69 0.34 6 0.66 0.51 0.66 0.51 5 1 .GO 0.00 1 

### .oo 0.90 1 0.00 0.00 0.00 0.00 151 0.56 0.39 0.45 0.42

5 0.60 0.54 0.60 0.54 35 0.69 0.42 0.60 0.47 34 0.91 0.25 --0-88 0.28

Mean survival (percent: multiply by 100) S.D. = Standard deviation

16

### which hatched from collected eggs had 77% 24 

### hr latent survivability and 68% 48 

### hr survivability. Hatched Sciaenidae larvae exhibited 73%24 

### hr survivability and 63% 48 

### hr latent Survivability. Hatched larvae of the family Soleidae had 24 

### hr latent survivability of 98% 

### and 48 

### hr survivability of 96% 

### (Table 4). 

### The latent survival of. fish collected as larvae is summarized 

### in Table 5. 

### Anchoa mitchilli larvae had a latent 48 

### hr survivability of 73%, 

### and Sciaenidae larvae had a 

### latent 48 

### hr survival of 69%. 

### Cynoscion arenarius was the only other 1arva.e collected in sufficient numbers and had a 48 

### hr latent survival of 45%. 

### The latent survival of these abundant groups showed no significant differences between collected !arvae and larvae hatched from collected eggs. Invertebrate larvae generally showed higher latent survivability than did 

### fish larvae (Table 4). 

### Caridean zoea had 24 

### hr latent survivability 

### of 78% 

### and 48 

### hr survivability of 67%. 

### Xanthiidae zoea had 24 

### hr latent survivability of 88% 

### and 48 

### hr survivability of 80%. 

### Pinnotheridae zoea had 24 

### hr latent survivability of 85% 

### and 48 

### hr survivability of 71%. Grapsidae larvae were the only megalops collected in sufficient numbers for meaningful latent survivability analysis; they exhibited 98% 

### and 95% 24 

### hr and 48 

### hr'latent survivability, respectively.
4.2. 

### Organism Return Discharge Mean initial survivability of selected taxonomic groups collected at the ORD station is given in Table 6. 

### Initial survivability and percent hatchability of 

### selected eggs collected from day and night sampling, March-September 1985 

### are presented in Appendix 3, 

### Tables B-1 

### through B-8. 

### Appendix B, 

### Tables B-9 

### through 8-14 

### show initial survivability 

### of selected fish larvae collected from day and night sampling (March-September). Initial survivability of selected invertebrate 

### 1 arvae collected from day and night sampl ing (March-September) is given in Appendix 

### B, Tables B-15 

### through B-21. Two 

### groups of eggs were collected in sufficient numbers for meaningful analysis (Table 5). 

### Engraulidae eggs were collected mostly during the day and had mean initial survivability of 29%; 

### surviving eggs had a hatchability exceeding 90% 

### (Table 13). 

### Sciaenidae eggs had mean initial survivabiiity of

1 f 17

# 90

### -- --



### -- 

### --

-- 

### -- --



### --

-- 

### 7 I 1


### Table 6. 

### Summary data of 

### initial survivability tests at the Organism Return Discharge Station, Big Bend Power Station (1985).



### FISH 

## - EGGS :



### CLUPEIDAE ENGRAULIDAE SCIAENIDAE 

### SOLE

### IDAE LARVAE :



### CLUPEIDAE ENGRAULIDAE ATHERINIDAE SPAR

### IDAE Lagodon rhomboides SCIAENIDAE Bairdiell a 

### chrysoura Pogonias cromis Cynoscion nebulosus Cynoscion arenarius Leiostomus xanthurus BLENNIIDAE GOBI IDAE SOLEIDAE INVERTEBRATES ZOEA:

PENAE

### IDAE CAR1DEA PORTUNIDAE PAGURIDAE XANTHIIDAE PINNOTHERIDAE GRAPSIDAE 

### MEGALOPS :



### XANTHIIDAE GRAPSIDAE



### i INITIAL SURVIVABILITY Total # 

### Organisms Tested Mean S.D.

9 0.49 0.13 3,522 0.29

. 0.21 16,693 0.40 0.04 23 0.56 0.20

2 0.00 0.00 271 0.58 0.60

284 0.56 0.16

11 0.00 0.00

1 1-00 0.00 

### 5 0.00 0.00 1. 0.00 0.00

2 0.50 0.00 458 0.70 0.02 7 1.00 0.00 21 0.79 0.23 7 

### ,576 0.90

.. - 0.01 29 

### ,845 0.83 0.07 442 0.96 0.06
5 0.50 0.71 109 0.76 0.08

### 40%, 

### were collected 

### in higher numbers during the night and had a hatchability of approximately 80%. 

### Of the fish larvae collected at the ORD, 

### only two groups were collected 

### in sufficient numbers for meaningful analysis (Table 6). 

### Engraul idae larvae, of 

### which 271 

### organisms were tested, exhibited initial survivability of 58%. 

### Sciaenidae larvae had initial sdrvivability of 56%. 

### For both of 

### these groups there were not sufficient numbers represented in 

### the collections for a determination of day/night or monthly comparisons 

### in survivability. 

# i Invertebrate larvae exhibited the highest survivability of all larvae collected at the ORD .(Table 6). 

### Four groups of invertebrate larvea were 

# 1 considered for detailed analysis of initial survivability. Caridean zoea 

### had 70%, 

### with no apparent difference between day/night or monthly collections. Initial survivability for Xanthidae zoea was 90%, 

### with more larvae being collected during the day but no apparent difference in day/night initial survivability. Pinnotheridae zoea, the most abilndant zoea collected at 

### the ORD, had mean initial survivability of 83%. 

### More of 

### these zoea were collected during the day, with no apparent difference in survivability between day and night. Grapsidae larvae had initial survivability of 96% 

### 'butwere not present in 

### sufficient numbers for accurate day/night or monthly survivability determinations. No 

### megalops were collected in sufficient numbers for meaningful survivability analysis (Table 5). 

### Mean 24 

### hr and 48 

### hr latent survival data of selected taxonomic groups collected at the ORD is summarized in 

### Tables 7 

### and 8. 

### The 24 

### hr and 48 

### hr latent survivability of selected fish larvae hatched from' eggs collected in day and nil;ht samplings for March-September 1985 

### is shown in Appendix B, 

### Tables B-22 

### through B-28. 

### The 24 

### hr and 48 

### hr latent survivability of fish larvae collected at the ORD, 

### March through September, is shown in Appendix 

### B, Tables 8-29 

### through 6-35. 

### Latent survivability of selected invertebrate larvae for 24 

### hrs and 48 

### hrs collected 

### in day and night samplings, March-September, is presented in 

### Appendix B, Tables 6-36 

### through B-42. 

### Of the fjsh larvae hatched from collected eggs, three were of sufficient numbers for meaningful analysis (Table 7). 

### Engraulidae larvae had 24 

### hr latent survivability of 86% 

### and 43 

### hr latent

19

### --

--

--


### -- --

--


### --

-- 

### --
--
-- -- 

### -- -- 

### --

--


### --
-- --

--


### --
-- Table 7. 

### Summary.data of 

### latent survivability tests for 24 

### and 48 

### hours, Organism Return Discharge Station, Big Bend Power Station (1985). 

### LATENT SURVIVABILITY Total # 

### Organi srns 24 

### hrs 48 

### hrs Tested Mean S.D. Mean S.D. FISH 

## - EGGS:



### CLUPE

### IDAE 2 0.50 0.00 

### ENGRAULIDAE 128 0.44 0.06 

### SCIAENIDAE 505 0.42 0.01 

### SOLE

### IDAE 7 0.65 0.02 

### LARVAE:



### CLUPE

### IDAE 3 1.00 0.00 1.00 0.00 

### ENGRAULIDAE 1 $249 0.86 0.05 0.65 0.11 

### ATHERINIDAE 4 0.16 0.23 0.16 0.23 

### S

### PARIDAE ” - -I 

### Lagodon rhornboides SCIAENIDAE 2 1,376 1.00 0.83 0.09 0.06 1-00 0.66 0.00 0.00 

### Bai rdiel 1 

### a chrysoura Pogonias cromis 1 1.00 9.00 1 

### .oo 0.00 12 0.34 0.47 0.34 0.47

2 0.50 0.71 0.50 0.71 

### GOBI IDAE 7 0.67 0.47 0.67 0.47 

### SOLEIDAE a73 0.93 0.09 0.90 0.11 

### Cynoscion nebulosus



### INVERTEBRATES ZOEA:

PENAEIDAE 27 0.99 0.00 0.97 0.03 

### CARIDEA PORTUNIDAE 2,118 6 0.79 0.88 0.00 0.17 0.66 0.90 0.00 0.14 

### PAGUR

### IDAE 76 0.81 0.09 0.68 0.14 

### XANTHIIDAE PINNOTHERIDAE GRAPS

### IDAE 1,799 3,674 192 0.81 0.80 0.94 0.04 0.00 0.03 0.71 0.65‘ 0.89 0.06 0.00 0.03 

### MEGALOPS:

XANTHIIDAE 72 0.93 0.07 0.92 0.08 GRAPS

### IDAE 1,469 0.96 0.03 0.94 0.04 

### JUVENILES:

Lo1 1 

### iguncula brevis 4 0.00 0.00 0.00 0.00

### --

--
--

--

-- --

--


### --

--

-- 

### --



### --

--



### --

-- 

### --

--
--

--

-- Table 8. Summary of 

### the latent survival of fish larvae from Big Bend Organism Resturn Discharge Station for 24 and 48 hours, for a17 samplings. (1.00 = 100% survival).
24 

### hrs 48 

### hrs No. Taxa Tested Mean S.D. Mean S.D. Clupeidae Anchoa mitchilli At tierinidae Sparidae Laqodon rhomboides Sciaenidae Bairdiella chrvsoura Poqonias crornis Cvnoscion nebulosus Cvnoscion arenari

### us Leiostomus xanthurus B1 enni idae Gobi idae Soleidae 0.93 0.16 0.76 0.33

1 

### .oo 0.00 1.00 0.00 0.85 0.28 0.70 0.37 1.00 0.00 1 

### .oo 0.00 1 

### .oo 0.00 1 

### .oo 0.00

0.41 0.46 0.33 0.44

0.50 0.70 0.50 0.70 0.60 0.54 0.40 0.54 1 

### .!lo 0.00 0.95 0.22

Mean 

### survival (percent: multiply by 100) S.D. = Standard deviation

21survivability of 65%, with no difference in survivability between day and night samples. Sciaenidae larvae had 24 hr latent survivability of 83% and 48 hr latent survivability of 66%; there 

### was no apparent difference between day/night or monthly samples. Soleidae larvae had 24 hr and 48 hr latent survivability of 93% and 90%, respectively. Almost all of 

### the Soleidae larvae 

### were collected during the day; there was no difference in survivability between monthly samplings. The 24 hr and 48 hr latent survivability of fish larvae collected at the ORD 

### is summarized in Table 8. Anchoa mitchilli larvae had 

### a 48 hr latent survivability of 76%, and Sciaenidae larvae had a 48 hr latent survivability.of 70%. The mean latent survival values for these larvae slightly exceeded the latent survival values for these larvae hatched from eggs collected at 

### the ORD, though the differences were not significant.
I. Latent survivability of the invertebrate larvae was similar 

### to that of fish larvae (Table 7). Caridean zoea had 24 

### hr and 48 hr latent survivability of 79% and 66%, respectively. Equal numbers of zoea were tested from day and night samples with no apparent difference in Survivability. Xanthidae zoea had 24 hr latent survivability of 81% and 48 hr latent survivability of 71%. There was no difference in numbers 

### or survivability of these zoea between day and night samplings. Pinnotheridae zoea exhibited 24 hr and 48 hr latent survivability of 80%.and 65%, respectively. There was no apparent difference between day and night or monthly samplings for these zoea. The only megalops which were collected in sufficient numbers for analysis were thos. of 

### the family Grapsidae, which had 24 hr latent survivability of 96% and 48 hr survivability of 94% (Table 7). There was no difference in latent survivability between day and night or monthly samplings of these megalops. 4.3. Control Mean initial survivability of selected taxonomic groups collected at the control station is summarized in Table 3. Initial survivability and percent hatchability of selected eggs collected from day and night sampling for March-September 1985 are shown in Appendix C, Tables C-1 through C-7. Appendix C, Tables C-8 through C-14 present the initial survivability of selected larvae

22

### -- --

-- -- 

### --



### -- -- 

### -- Table 9. 

### Summary data of 

### initial survivability tests at the Control Station, Big 

### Bend Power Station (1985). 

# 1 

# 1 FISH 

## - EGGS:

CLUPEIDAE ENGRAULIDAE SCIAENIDAE SOLE

### IDAE LARVAE:

C

### LU

### PE

### IDAE ENGRAULIDAE ATHERINIDAE SPARIDAE Laqodon rhomboides SCIAENIDAE Bairdiella chrysoura Pogonias cromis Cynoscion nebulosus Cynoscion arenarius Leiostomus xanthurus BLENNIIDAE GOBIIDAE 

# I

f 

# . SOLEIDAE 

# ? INVERTEBRATES ZOEA:

PENAEIDAE CARIDEA PORTUNIDAE PAGURIDAE XANTHIIDAE PINNOTHERJDAE GRAPSIDAE MEGALOPS:

XANTHIIDAE GRAPSIDAE INITIAL SURVIVABILITY Total # 

### Organisms Tested Meam S.D.

60 0.73 0.38 16,378 0.72 0.31

5,872 0.72 0.05 3 1.00 0.00

303 0.16 0.09 3 0.00 0.00
95 0.22

12 0.60 0.57 14 0.50 0.71 35 0.00 0.00

347 0.65 0.09

14 1.00 0.00

5 1-00 0.00 671 0.88 0.10 2,987 0.77 - - 0.13 37 0.81 0.11

1 3-00 0.00 24 0.84 0.08

23 

# GT

I
i


### 1 

## 7



## i from day and night collections for March-September. Initial survivability of selected invertebrate 1

### arvae col1ected from day and night sampli

### ng, March-September is 

### given in Appendix 

### C, Tables C-15 

### through 8-21. 

### Two taxonomic groups of eggs were collected in sufficient numbers for initial survivability analysis; those were Engraulidae eggs with mean initial survivability of 72% 

### (Table 9) 

### and percent hatchability of greater than 95% 

### (Table 13), 

### and Sciaenidae eggs with mean initial survivability of 72% 

### and hatchability of greater than 90%. 

### For Engraulidae eggs there was no difference in survivability between day/night or monthly samples. Most Sciaenidae eggs were collected during night samplings; there was no apparent difference in survivability between day/night or monthly samplings. Fish larvae collected during'the control sampling were not present in sufficient numbers for meaningful initial survivability testing. Engraulidae larvae had initial survivability of 16%; 

### however, only 303 

### individuals were available for this testing. Sciaenidae larvae exhibited 85% 

### initial survivability, but only 95 

### individuals were tested. Two taxonomic groups of selected invertebrate larvae were present in sufficient numbers for meaningful initial survivability testing (Table 9). 

### Xanthidae zoea had mean initial survivability of 85%, 

### and there was no difference 

### in initi-a1 survivability between day/night or monthly samples. Pinnotheridae zoea had mean initial survivability cf 72%, 

### with no apparent difference in survivability between day/night or monthly samples. The 24 

### hr and 48 

### hr latent survivability of 

### selected taxonomic groups is 

### summarized in Tables 10 

### and 11. 

### The 24 

### hr and 48 

### hr latent survivability of fish larvae hatched from collected eggs for day/night sampling from March-September is 

### shown in Appendix C, 

### Tables C-22 

### through C-28. 

### The 24 

### hr and 48 

### hr 

### latent survivability of fish larvae collected at the control station, March through September, is given in Appendix 

### C, Tables C-29 

### through C-35. 

### For day and night sampling, March-September, the 24 

### and 48 

### hr latent survivability of selected invertebrate larvae is presented in Appendix 

### C, Tables C-36 

### to C-42. 

### Hatched Engraulidae larvae had 24 

### hr latent survivability of 70% 

### and 48 

### hr latent survivability of 59% 

### (Table 10). 

### Most individuals tested were from

i 24 

# J?

### --

-- --
-- --

-- --
-- --



### -- --

-- 

### -- -- 

## -- --

--

-- 

### --
--

-- 

### Table 10. 

### Summary data of 

### latent survivability tests for 24 

### and 48 

### hours, 

### Control Station, Big Bend Power Station (1985). 

### LATENT SURVIVABILITY Total # 

### Organisms 24 

### hrs 48 

### hrs 

### Tested Mean 

### S.D. Mean S.D. FISH 

## - EGGS:

0.74 0.59 0.46 

### CLU

### PEI

### DAE 

### ENGRAULIDAE SCIAENIDAE SOLE

### IDAE LARVAE:



### CLUPEI

### DAE ENGRAULIDAE ATHERINIDAE SPARIDAE Lagodon rhomboides S C

### IAENIDAE

GOBI IDAE SOLE

### IDAE INVERTEBRATES ZOEA:

PENAEIDAE CARIDEAN PORTUNIDAE PAGURIDAE XANTHIIDAE PINNOTHERIDAE GRAPSIDAE 

### MEGALOPS :



### XANTHJIDAE GRAPS

### IDAE JUVENILES:

Lo11 i

### guncula 

### brevis 0.00 0.21 0.06 0.00
0.18 0.21 0.17 1 30 109 1
23 1,361 49 0.50 0.51 0.56 0.50
0.88 0.70 0.52 0.00 0.61 0.50 0.00 0.11 0.50 0.00 0.77 0.50 1 902 6
0.00 1

### .oo 3 1.00 0.65 0.75 0.78 0.78 0.95 147 156 285 0.08 0.31 0.01

1 

### .oo 0.88 0.67 0.82 0.85 0.74 0.88
1.00 1.00
0.00 c.00 0.C8 0.47 0.10 0.06 0.13 9.00
0.00 0.00
0.00 0.00 0.03 0.00 0.00 0.05 0.06 0.03
0.00 0.00
0.00 1.00 0.95 1.00 1 

### .oo 0.90 0.86 0.93 1 

### .oo 1 

### .oo
0.00 1 1,362 14 44 750 1,281 107

28 261
1

### --



### -- --



### -- -- 

### --



### -- -- -- 

### -- Table 11. Summary of the latent survival of fish larvae from Big Bend Control Station for 24 and 48 hours, for 

### a1 1 samplings. (1.OO = 100% survival).
24 hrs 48 hrs No. Taxa Tested Mean S.D. Mean 

### S.D. 

# 1
C1 upeidae 

# 1 Anchoa mitchilli 209 0.61 0.38 0.52 0.37 Atherin idae 42 0.35 0.39 0.29 0.40 

## I I Sparidae Laqodon rhornboides 1 0.00 0.00 0.00 0.00 

## f 1 Sciaenidae 100 0.78 0.38 0.74 0.33 Bairdiel 1a chrysoura Poqonias cromis

= 

# . i Cynoscion arenari

### us Leiostomus xanthurus B1enniidae 141 0.82 0.27 0.67 0.36 

### P 

## t Gobiidae 144 0.77 0.29 0.74 0.29 Soleidae 10 1 

### .oo 0.00 0 :77 0.44 Cvnoscion nebulosus

Mean survival (percent : mu1 tiply by 100) S.D. = Standzrd deviation

26

### day samples, and there was no apparent difference between monthly samplings. Hatched Sciaenidae larvae had 24 

### hr latent survivability of 77% 

### and 48 

### hr latent survivability of 61%, 

### with higher survivability in night samples, when compared to day samples. The 48 

### hr latent survival of collected Anchoa mitchilli larvae was 52%, 

### and that of collected Sciaenidae larvae was 74%. 

### These values were similar to those of the hatched 

### 1 arvae. Of the invertebrates collected for latent mortality testing, three taxonomic groups were considered for detailed analysis (Table 10). 

### Caridean zoea had 24 

### and 48 

### hr latent survivability of 95% 

### and 88%, 

### respectively. There was no difference in latent survivability between day/night or monthly samplings. Xanthidae zoea, of which 750 

### were tested, had 24 

### hr latent survivability of 90% 

### and 48 

### hr latent survivability of 85%. 

### There was no apparent difference between day/night or monthly samplings. Pinnotheridae zoea exhibited 24 

### hr latent survivability of 86% 

### and 74% 

### for 48 

### hr latent survivability. Again, there was no apparent difference in latent survivability between day/night or monthly sampl incjs. blegalops were not collected in sufficient numbers for detailed analysis.
4.4. 

### Survivability Comparisons Between Sites Table 12 

### summarizes survivability data for the abundant taxa at the three sampling sites. Though not statistically significant, engraulid and sciaenid eggs exhibited lower survival rates 

### in comparison to control organisms’ survivability. Fish larval and zoea1 survivability were similar at all three

.. . 

### sites.
4.5. 

### Survivability and Abiotic Parameters The mean values for abiotic parameters measured March through September are summarized in Table 2. 

### Mean temperatures ranged from a low of 22.2 

### OC to a high of 32.5 OC. 

### There was a correlation found between survivability and temperature in the prototype study, though the correlation was not a strong one. t 

### In this study, the temperatures followed a typical seasonal trend, and there was t 

### no significant correlation between temperature and 

### survivability. Salinity andTable 12. Comparison of percent initial and latent (48 hrs) survival of selected taxa between sampling sites at Big Bend Power Station. Control Screenwash 

### ORD Taxa Initial Latent Initial Latent Initial Latent 

### EGGS:

Engraul idae 72 51 48 45 29 44 Sciaenidae 72 56 63 44 56 47 FISH LARVAE:

Engraul idae 16 59 16 68 58 65 Sciaenidae 85 61 61 63 56 66 ZOEA:

Caridea 65 88 72 67 70 66 Xanth idae 88 85 93 80 90 71 Pinnatheridae 77 74 89 71 83 65

28

### -- 

### --

-- 

### --

-- 

### -- -- Table 13. 

### ,,dtcha, laby Summary for 

### FMS Survivability study stations. SCREENWASH STATION 

### (n> 

### DAY

### * (n) N

### I

### GHT" C1upeidae 6 0.83 

## + .289 5 0.71 

### -+ .057 

### Engraul i

### dae 1299 0.82 

### 7 .097 562 0.66 

### 7 -442 

### Sciaenidae 1129 0.84 

# z .198 1416 0.76 

### T .042 

### Soleidae 815 0.99 .006 64 0.93 

### T 

### - .145


### ORGANISM RETURN DISCHARGE STATION (n> DAY (n> NIGHT 

### C1,upe i

### dae 2 

### Engraul i

### dae 1140 0.91 

## + -043 219 0.95

- 

### + .062 

### Sciaenidae 881 0.90 

### T .082 1014 0.71 

### + .076 

### Soleidae 817 0.99 .006 60 0.92 

### T

- -098


### CONTROL STATION NIGHT 

### C1upeidae 5 0.80 

### + .192 

### Engraul idae 988 0.98 - 

### + -018 398 0.99 

# z .015 

### Sciaenidae 357 0.92 

### 2 -038 555 0.89 

### + .on Soleidae 4 0.80

- -320 

### (n) = 

### Number of 

### organisms tested

Mean Survival (percent: multiply by 100) 

### 5 

### Standard Deviation

29 

# 59-I

1 

# I dissolved oxygen fol lowed expected seasonal trends, with no correlation between survivabili

### ty and these parameters.
4.6. 

### Survivability Comparisons to Prototype Study A 

### comparison of 

### initial survival for selected taxa during the prototype and the 

### FMS Survivability studies is given 

### in Table 

### 14. The initial survival of abundant eggs was similar 

### in both studies, with differences being species specific. Fish larvae had a significantly lower survival during the prototype study, which may be attributed to differences in the sampling techniques between the two studies. 

### Fish larvae survival was species specific in both studies. The initial survival of the invertebrates was higher in the prototype study than 

### in the 

### FMS Survivability study. These differences were species specific, with some variability seen between stations and species. A 

### comparison of fish egg hatchability and latent 48 

### hour survival for selected taxa collected during the prototype and FMS 

### Survivability FMS studies is 

### given in Table 15. 

### In general, hatchabiiity was high during the FMS 

### Survivability study, as predicted during the prototype studies. Latent survival of fish larvae was low during the prototype study, and significantly higher during the 

### FMS Survivability study. These results follow those obtained for the initial survival of fish larvae, and can be explained by the differences in the sampling techniques. The latent survival of 

### the invertebrates was the highest of any organisms tested during both studies. In general, the latent survival was slightly lower during the FMS 

### Survivability study, with variations seen

.. . 

### between species and stations.

5.0 

### DISCUSSION 5.1 

### Overall SurviVal Results of this study indicate that of all the taxonomic groups studied, the invertebrates had the highest survival. Among invertebrates, the megalops stages exhibited the best survival rates. Fish 

### larvae had low survivability especially fragile fish, such as many of the Engraulidae (anchovies).

..
30

### Table 

### 14.

Fish Eggs: 

### Anchoa mitchilli Sciaenidae Fish Larvae: Anchoa mitchilli Sciaenidae Invertebrates: Caridea Xant

### h i

### dae Pinnotheridae Comparison of 

### initial survival (%) 

### for selected taxa during the prototype and FMS 

### Survivability studies.

Prototype FMS 

### Survivability Study Screenwash Screenwash 

### ORD Ccntrol
43 75



### 2 19
94 99 100 48 29 72 63 40 72
16 58 16 61 56 85
72 70 65 93 90 88 99 a3 77

31

# 1

f



# t

i Table 15.



## - Taxa Fish Eggs: 

### Anchoa mitchilli Sciaenidae

Fish Larvae: Anchoa mitchilli Sciaenidae Invertebrates: Caridea Xanthidae Pinnotheridae Comparison of fish egg 

### hatchability and latent 48 

### hour survival (%)for selected taxa during the prototype and FMS 

### Survivability studies.

HATCHABILITY Prototvpe FMS Survivabilitv Study 

### Screenwash Screenwash ORD 

### Control
80 

### 74 93 98 95 80 80 90


### LATENT 48-HOUR SURVIVAL
22 68 65 59 11 63 66 61
85 67 65 88 96 92 80 71 71 65 85 

### 74

### The proportion 

### of organisms a1 ive at 24 

### hrs and 48 

### hrs was used as a measure of latent survival. Results indicated that invertebrates had the highest latent survival 

## , with fish larvae having a lower latent survivability. Initial survivability as well as hatchability of eggs was determined. Hatchability is a 

### measure of the viability of the eggs. Egg survival was relatively low, but hatchability of those surviving eggs was high. 5.2 

### Effectivenes of 

### FMS The effectiveness of the fine mesh screens was determined by comparing survivability of eggs and larvae collected from the screenwash, organism return discharge (ORD), and control stations. Results of the testing showed a 

### lowetinitial and latent survivability cjf 

### organisms from the ORD station than either the screenwash or control stations. However, comparing results of the control testing and screenwash testing indicates that natural mortal ity not associated with the fine mesh screens may be a factor contributing to the mortalities observed among organisms collected by the fine mesh screens. Results also indicate that survivability is 

### species and lifestage speciffc. This includes initial as well as 

### latent survivability. There was no apparent difference in survivability between day and night samples, and no. difference in survivability between months.
5.3 

### Comparison to Prototype Results obtained during this study were comparable to preliminary results obtained in the prototype study (TEC, 1980b). The initial.-survivability of eggs reported in this study were slightly higher than those reported in the prototype study, though hatchability was very similar.

. 

### The initial and latent survivability of fish reported in the prototype study was very low 

### compared to what was 

### reported in this study. Results obtained in this study for 

### the control station were similar, though higher, to the control survivability values in the prototype study, but screenwash arid ORD survivability values were significantly higher than in the prototype. The high survivability of 

### invertebrate stages was comparable in both the studies. In general, results obtained during the

33

### full-scale study were equal to those predicted in 

### the prototype study. In some cases, results obtained exceeded those expected, with these differences being attributed to differences in sampling techniques (i.e., nets in the FMS 

### Survivability study versus sampling boxes in the Prototype study).

34

### 6.0 

### Summarv and Conclusions

1. A 

### Fine Mesh Screen (FMS) study was conducted from March through September, 1985 

### representing one 

### full spawning season, after Unit 4 

### began commercial operation (February 25, 1985), 

### to evaluate the effectiveness of FMS in reducing entrainment. 2. 

### Sampling was conducted at three stations: The screenwash in front of 

### the screens, the end of the Organism Return Discharge (ORD), 

### and 

### in the intake canal. Methods used were similar to those used during the FMS Prototype study conducted in !980 (TEC, 1980b). 3. 

### Certain species which are found in high abundance or are of commercial or forage value were targeted as Representative Important Species (RIS) 

### for this study, and initial and latent survivability of 

### these taxa were analyzed for the screenwash, organism return discharge, and control stations. 4. 

### Initial survivability of Anchoa mitchilli eggs from the screenwash was 48%; 

### from the ORD 29%; 

### and from the control 72%. 

### 5. 

### Initial survivability of Sciaenidae eggs from the screenwash was 63%, from the ORD 40%, 

### and from the control 72%. 

### 6. 

### Initial survivability of select invertebrates from the screenwash was 87%, 

### from the ORD 85%, 

### and from the control 80%. 7. 

### Initial survivability of Anchoa mitchilli larvae from the screenwash was 16%, from the ORD 58%, and from the control 16%. 

### 8. 

### Initial survivability of Sciaenidae larvae from the screenwash wa: 61%, 

### from the 

### ORD 56%, 

### and from the control 85%. 9. 

### Hatchability of 

### Anchoa mitchilli eggs from the screenwash was 74%,

.. - 

### from the ORD 81X, 

### and from the control 99%. 10. 

### Hatchability of Sciaenidae eggs from the screenwash was 80%, 

### from the ORD 81%, 

### and from the control 91%. 

### 11. 

### Latent survivability (48 

### hr) of Anchoa rnitchilli larvae from the screenwash was 68%, 

### from the ORD 65%, and 

### from the control 59%. 12. 

### Latent survivability (48 

### hr) of Sciaenidae larvae from the screenwash was 63%, from the 

### ORD 66%, and from the control 61%. 13. 

### Latent survivability (48 

### hr) of select invertebrate larvae from the screenwash was 73%, from the ORD 67%, 

### and from the control 82%. 14. 

### There was no correlation found betwesn abiotic parameters measured and survivabi 1 i

### ty.
3515. 

### Initial survivability and hatchability of eggs in the FMS 

### Survivability Study was comparable to initial survivability values reported 

### in the prototype study conducted during 1980. 16. 

### Initial and latent survivability of fish eggs and larvae in the 

### FMS Survivability Study was significantly higher than values reported in the prototype study. These differences are attributed to differences in sampling technique. 17. 

### Latent survivability of 

### invertebrate larvae in the FMS 

### Survivability Study was comparable to values reported in the prototype study. 18. 

### Results indicate that survivablity is species and 

### 1 ifestage specific. 19. 

### There was no significant difference in survival rates between stations. 20. 

### Survival rates during the FMS 

### Survivability Study were generally as predicted by the prototype study, and in some cases exceeded predicted Val ues.

H 

# f

36 I7.0 

### LITERATURE CITED Tampa Electric Company, 1980a. 316(a) and (b) Demonstration, Big Bend Station 

### - Unit 4, 

### Tampa Electric Company (August 1980). 

### Prepared by Stone and Webster Engineering Corporation. Tampa Electric Company, 1980b. 316(a) and (b) Demonstration, Big Bend Station 

## - Unit 4, 

### Tampa Electric Company, Fine Mesh Screen Study Report (November 1980). 

### Prepared by Stone and Webster Engineering Corporation.

i



### i

37I



### APPENDIX A 

### FMS SURVIVABILITY STUDIES DATA SCREENWASH STATION



# f

### -- -- --



### -- -- 

### -- -- -- 

### --

--



## -- -- 

### --

-- -- -- 

### -- -- 

### -- 3

Table A-1. Initial survival and hatchability of fish eggs, Screenwash Station, Big Bend 

### Power Plant, for March 1985, day and night sampling. (1.00 100% surviVal and hatchabi 

### 1ity)

# . = 

# f DAY 

## -

CLUPE

### IDAE ENGRAULIDAE SCIAENIDAE SOLEIDAE

NIGHT



### CLU

### PEI

### DAE ENGRAULIDAE SCIAENIDAE SOLE

### IDAE.



## - No. Initial Organi

### sms 

## - SurviVal Total

Tested X 

### S.D. No.

97 0.98 0.03 2314 0.60 0.29 205

961 0.70 

### 0.34 178



### No. Initial Organisms Survival Tota1

Tested X 

### S.D. No.
116 0.62 0.00 

# - Hatchability X 

### S.D.

0.78 0.28 0.83 0.30

Hatchabi 1it y


### - X 

### S.D.

2278 0.67 0.31 201

..0 

## . .84 0.23 4572 0.69 0.32 239 0.78 0.12



### - X = Mean survival (percent: multiply by 100). S.D. = Standard deviation.

## --

-- -- --

-- -- --

-- 

### Table A-2. Initial survival and hatchability of fish eggs, Screenwash Station, 'Big Bend Power Plant, for April 1985, 

### day and night sampling. (1.00 100% 

### survival and hatchability). = 

### APRIL DAY


### - No. Initial Organisms 

## - Survival Total

Tested X 

### S.D. No.

ENGRAULIDAE 7838 0.79 0.29 257 

### SCIAENIDAE 1905 0.73 0.36 312 

### SOL 

### E IDAE 18 1 

### .oo 0.00



### NIGHT No. 

### Initial Organi

### sms



### Tested 

## - Survival X 

### S.D Total No. CLUPEIDAE 

### 1 1 

### .oo 0.00 

### Hatchability X 

### S.D.

0.89 0.22 0.90 0:28



## - Hatchabil i

### ty X 

### S.D

ENGRAULIDAE 3411 0.67 0.30 324 - 0,81 0.15 

### SCIAENIDAE 24332 0.82 0.12 269 0.76 0.22 

### SOLE

### IDAE 1 1 

### .oo 0.00 35 0.97



## -

- X = 

### Mean survival (percent: multiply by 100). 

### S.D. = 

### Standard deviation.

### --

-- -- -- --
-- --



## -- -- -- 

### -- Table A-3. 

### Initial survival and hatchability of fish eggs, Screenwash Station, Big Bend Power Plant, for Hay 1985, 

### day and night sampling. (1.00 = 100% 

### survival and hatchability).



### DAY 

## -

CLUPEI

### DAE ENGRAULIDAE SCIAENIDAE SOLEIDAE

NIGHT

C

### LUPEI

### DAE ENGRAULIDAE 

### SC

### I

### AENI

### DAE SOLEIDAE



### - MAY


### - No. Initi a1 Organi srns



### Tested 

## - Survival X 

### S.D. Total 

### No. 

## - Hatchabi

### 1 i 

### ty X 

### S.D. 2 

### 0.50 0.71 2 0.50 0.71 2261 0.81 

### 0.23 21 1 0.64 0.11 2056 0.64 

### 0.39 208 0.40 0.36 8 1.00 0.00 87 1 

### .oo

No. Initial Organi srns 

### - SurviVal Total


## - Hatchability Tested X 

### S.D. No. X 

### S.D. 3 0.75 41 0.18 0.32 54 1

.. ­ 7628 0.83 

### 0.16 184 0.82 0.15 9 1.00

- X = 

### Mean survival (percent: multiply by 100). 

### S.D. = 

### Standard deviation. 

### i

### --



### -- -- 

### -- --



### -- -- --

-- -- -- -- Table A-4. 

### Initial survival and'hatchability of fish eggs, Screenwash Station, 

## f li Big Bend Power Plant, for June 1985, day and night sampling. (1.00
= 

### 100% 

### survival and hatchability).


### JUNE DAY 

## - No. Initi 

### a1 Organisms

Tested 

## - Survival X 

### S.D. Total No.
C

### LU

### P

### EIDAE 1 1 

### .oo 0.00 1 

### ENGRAULIDAE 89 

### 0.90 0.05 102 

### SCIAENIDAE 1503 0.57 

### 0.46 105 

### SOLEIDAE 136

NIGHT No. Initi a1 Organisms ' 

## - Survival Total

Tested X 

### S.D. No.



### ENGRAULIDAE 9 0.00 

### 0.00 

### - Hatchability X 

### S.D. 3 

### .oo 0.85 3.34 

### 0.96 0.09 0.99



## - Hatchability X 

### S.D.­

.. - 

### SCIAENIDAE 4399 0.64 

### 0.28 1

### 83 0.78 0.14 SOL

### EI

### DAE. 6 1-00



### -

- X = 

### Mean survival (percent: multiply by 100). 

### S.D. = 

### Standard deviation.

f 

# Y.4

### --

--



### -- 

## -- -- --


### -- -- --

-- -- 

### -- -- Table A-5. 

### Initial survival and hatchability of fish eggs, Screenwash Station, Big Bend Power Plant, for July 1985, 

### day and night sampling. (1.00 = 

### 100% 

### surviVal and hatchabi 

### 1i

### ty) 

## . JULY 

### DAY 

## - No. Initial Organisms 

### - SurviVal Total 

## - Hatchability Tested X S.D. No. X S.D.


### C

### LU

### P

### EIDAE 2 0.50 0.71 3 1-00 

### ENGRAULIDAE 1174 

### 0.80 0.27 23 1 0.93 0.32 

### SCIAENIDAE 168 0.68 0.39 121. 0.94 0.29 

### SOLE

### IDAE 1 1

## .oo 0.00 237 0.99



### NIGHT 

### No. Initial Organisnis


## - Survival Total 

## - Hatchability Tes

### ted X 

### S.D. No. X S.D. 

### CLUPE

### IDAE 2 0.67 

### ENGRAULIDAE 4 0.00 0.00

.. . 

### SCIAENIDAE 1150 0.57 0.21 178 0.73 0.13 

### SOLEIDAE -2 0.67



## -

- X = 

### Mean survival (percent: mu1 tiply by 100). 

### S.D. = 

### Standard deviation.-- 

### -- -- 

### -- -- --



### -- -- -- 

### --

--



### -- -- 

### -- -- 

### -- 

### --
--



### -- -- 

### -- 

### -- -- -- Table A-6. Initial survival and hatchability of 

### fish eggs, Screenwash Station, Big Bend Power Plant, for 

### August 1985, 

### day and night sampling. (1.00 100% 

### survival and hatchability). = I 

## a AUGUST 

### DAY 

## - No. Initial Organ

### i sms 

## - Survival Total

Tested X 

### S.D. No. 

# 1 CLUPE

### IDAE ENGRAULIDAE 1741 0.78 

### 0.25 139 

### SCIAENIDAE 178 

### 0.64 0.34 

### 51 

### SOLEIDAE 255



### NIGHT No. Initial Organisms 

### - X 

### SurviVal S.D. Total Tested No.


### CLUPEIDAE 

# - Hatchability X 

### S.D.

0.82 0.10 

### 0.89 0.33 0.99



### Hatchabi 1 

### i 

### ty



### - X 

### S.D.

-



### ENGRAULIDAE 13 

### 0.00 0.00 2 

### ..o .DO SCIAENIDAE 2259 0.76 

### 0.14 164 0.69 0.21 

### SOLEIDAE



### -

- X = 

### Mean survival (percent: multiply by 100). 

### S.D. = 

### Standard deviation.

L/

## 9

### -- 

### -- -- -- -- 

### --

--

-- -- -- -- -- 

### --
--

-- -- -- -- Table A-7. 

### Initial survival and hatchability of fish eggs, Screenwash Station, Big Bend Power Plant, for September 1985, 

### day and night sampling. (1.00 100% 

### survival and hatchability). = 

### SEPTEMBER 

### DAY 

## - No. Initial Organisms 

## - SurviVal Total

Tested X 

### S.D. No.
CLUPEIDAE ENG

### RAULI

### DAE 714 0.46 

### 0.43 190 

### SCIAENIDAE

. 16 

### 0.33 0.58 154 

### SOLEIDAE 1 

### 0.00 0.00 100



### NIGHT No. Initi

### a7 Organ i

### sms 

## - SurviVal Tota1

Tested X 

### S.D. No.
CLUPEIDAE 

## - Hatchability X 

### S.D.

0.81 0.21 0.95 0.33 1 

### .oo



## - Hatchability X S.D.



### ENGRAULIDAE 11 0.04 

### 0.08 35 

### ..o .97 

### SCIAENIDAE 75 0.24 0.33 199 0.78 0.16 

### SOL

### EIDAE 12 1 

### .oo



## - X = 

### Mean survival (percent: multiply by 100).

- S.D. = 

### Standard deviation.

### --

-- 

### -- -- Table A-8. Initial survival of fish larvae from Big Bend Screenwash Station, day and night sampling, March 1985. (1.00 = 100% survival). INITIAL 

### SURVIVAL DAY NIGHT 

### No. No. Organisms Organisms 

# -

. 

## - Taxa Tested X S.D. Tested X S.D. 

### Cl upeidae Engraul idae Atherinidae Sparidae Lagodon

rhomboides Sciaenidae Bairdiella

chrysoura Poqonias

cromi

### s Cynoscion

nebul

### osus Cynoscion

arenari

### us Leiostomus

, xanthurus

.. -.- Blenniidae I 0.00 0.00 Gobiidae 35 0.00 0.00 Soleidae



## -

- X = .Mean survival (percent: multiply by 100). S.D. = Standard deviation.



# 4

### -- -- -- 

### Table A-9. 

### Initial survival of fish larvae from Big Bend Screenwash Station, day and night sampling, April 1985. 

### (1.00 = 100% 

### survival).

Taxa
Engraul idae

Sciaenidae Bairdiell a

ch

### rysoura

Gobi idae



## - INITIAL SURVIVAL DAY 

### NIGHT No. 

### No. Organisms



### - Organisms


## -

Tested X S.D. 

### Tested X S.D.

202 0.33 

### 0.58 224 0.23 0.39



### 416 0.81 0.20 42 0.94 0.13
1 0.00 0.00

4 0.00 0.00

- X = 

### Mean survival (percent: multiply by 100). S.D. = 

### Standard deviation.

### -- -- -- -- 

### -- 

## --

-- -- -- -- -- 

### --
-- -- -- Table A-30. Initial survival of fish larvae from Big Bend Screenwash Station, day and night sampling, May 1985. (1.00 = 100% survival).
INITIAL SURVIVAL DAY NIGHT 

### No. 

### No. Taxa Organisms Tested 

## - Organisms X 

### S.D. Tested 

## - X S.D. C1upeidae Engraul idae 124 0.00 0.00 125 0.00 0.00 Atherinidae Sparidae 1 

### O.OG 0.00

Sciaenidae 973 0.79 0.34 51 0.00 0.00 Bairdiella



### chry

### s

### oura 1 0.00 0.00
Cynoscion

nebulosus Cynoscion

arenari

### us 2 0.00 0.00 f

Gobiidae 9 0.00 0.00 7 0.00 0.00



### -

- X = Mean survival (percent: multiply by 100). S.D. = Standard deviation. 4

### --



### -- -- -- 

### -- Table A-11. Initial survival of 

### fish larvae from Big Bend Screenwash Station, day and night sampling, 

### June 1985. (1.00 = 100% survival).

Taxa
Engraul idae

Laqodon

rhomboides Sciaenidae Bairdiel 1a

chry

### sou

### ra Pogoni

### as

cromi 

### s Cynoscion

nebul osus Cynosci on

arenari 

### us Lei ostomus

xan

### thurus



## - INITIAL SURVIVAL DAY NIGHT 

### No. No. Organisms Organisms


## - - Tested X S.D. Tested X S.D.

49 0.07 0.22 129 0.18 0.30

55 0.96 0.06 6 0.00 0.00

0.50 0.71 0.39 0.34

- X = Mean survival (percent: multiply by 100). S.D. = Standard deviation).

# 1. 

# 1 1 Table A-12. Initial 

### survi a1 of fish larvae from Big Bend Screen\ ash St tion, day and night sampling, July 1985. (1.00-= 100% survival). 

# t INITIAL 

### SURVIVAL DAY 

### NIGHT No. 

### No. Oraani

### sms 

## - Orqan i 

### sms 

## - Taxa TGsted X S.D. TGsted X S.D. 

# 1 

# 1 C1upeidae Engraul idae 

# 1 At 

### heri

### ni dae 

# 1 Sparidae Lagodon

rhomboides 

# f 1 Sciaenidae 

# i Bairdiella 

### chry 

### sou

### ra Pogoni a s 

### I cromi

### s 

### 7 Cynoscion nebul

### osus 

# 1 Cynoscion arenari

### us 

# t Leiostomus xanthurus 

## i i Blenni idae 

# 1 Gobiidae Soleidae 

# i

­

- X = Mean survival (percent: multiply by 100). S.D. = Standard deviation. 

## i

### --

-- Table A-13. Initial survival of fish larvae from Big Bend Screenwash Station, day and night sampling, August 1985.
INITIAL SURVIVAL DAY 

### No. Organisms


## - Taxa Tested X S.D. C1 upeidae Engraulidae Atherinidae Sparidae Lagodon

rhomboides Sciaenidae Bairdiella



### chry

### s

### oura Poqoni as

cromi

### s Cynoscion

nebul

### osus Cynoscion

arenari

### us Leiostomus

xanthurus Blenniidae Gobi idae Soleidae



## - X = Mean survival (percent: multiply by 100).

- 

### S.D. = Standard deviation. (1.00 = 100% survival).

NIGHT 

### No. Organisms


## -

Tested X S.D.

3 0.00 0.00

.. -.­

0.25 

## iTable A-14. Initial survival of fish larvae from Big Bend Screenwash Station, day and night sampling, September 1985. (1.00 = 100% survival).
INITIAL SURVIVAL 

### DAY NIGHT 

### No. No. 

### i Organi 

### sms 

## - Organisms 

# - Taxa Tested X S.D. Tested X S.D.

1 

# i Clupeidae Engraulidae 

# 1 Atherinidae 

# t Sparidae Lagodon

rhomboides 

# 1 Sciaenidae 

### F Bairdiel 1a 

### e chrysoura Poqoni 

### as z f crorni

### s 

## a Cynoscion * nebul osus B 

### ; Cynoscion 

# r arenari

### us Leiostomus

xanthurus 

## i’ i f3l 

### enni idae 

# 1 Gobi idae 

### Soleidae 

# I

-

- X = Mean survival (percent: multiply by 100). S.D. = Standard deviation. 

# f
i t

ic 

### -- 

## -- 

## -- -- -- 

### --

-- -- --



### -- -- -- 

# I 

# I I 

# I 

# 1 1 

# 1 1 \ 

# f

ii 

# T 

# f

1 f



## 1 i Table A-15. Initial survival of invertebrate larvae from Big Bend Screenwash Station, day and night sampling, March 1985. (1.00 = 100% surviVal ) 

## .

~ INITIAL SURVIVAL DAY 

### NIGHT No. No. Taxa Organisms Tested 

# - X Organisms S.D. Tested 

## - X S.D. ZOEA Penaeidae Caridea 36 0.74 0.43 84 0.94 0.14 Portunidae 15 1 

### .oo 0.00 11 0.97 0.08

.. Paguridae 7 1 

### .oo 0.00 19 0.91 0.27 Xanthiidae 1069 0.98 0.05 1659 0.92 0.20 Pinnotheridae 1858 0.99 0.03 650 0.85 0.31 Grapsidae 1 1.00 0.00
MEGALOPS Xanthidae 8 1 

### .oo 0.00

JUVENILES Lo1 

### 1iquncula 1 1

## .oo 0.00

brevis



## - X = Mean survival (percent: multiply by 100).


### - S.D. = Standard deviation. 

# '

### -- -- 

### -- 

## --

--



### -- -- 

### --

-- -- -- 

### -- 

### -- --

-- 

### -- surviVal ) 

# . INITIAL SURVIVAL DAY No. No. Organisms Organisms


## - Taxa Tested X 

### S.D. Tested 

### ZOEA Penaeidae 1 ' Caridea 118 

### 0.90 0.29 34

3 Portunidae 

### 5 1

## .oo 0.00 

# 1 Paguridae 20 1.00 0.00 1 

# i Xanthiidae 2037 0.97 0.08 217 Pinnotheridae 1317 0.99 0.02 151 

# 4 Grapsidae 40 1

## .oo 0.00 3

z MEGALOPS 

# r Xanthi dae 1

I 

### f Grapsidae 4 1.00 0.00 6 JUVENILES Lo1 1 iquncul a

brevis



### -

- x = Mean survival (percent: multiply by 100). S.D. = Standard deviation.



# f Table A-16. Initial survival of invertebrate larvae from Big Bend Screenwash Station, day and night sampling, April 1985. (1.00 100% =
NIGHT



# - X S.D.



### 0.67 0.41

1-00 0.00 0.96 ' 0.13 0.93 0.17 0.67 0.58

1.00 0.00 1-00 0.00

### --



## --

-- --



### --



### -- --

--



### -- 

### --

--
--



### -- 

### --

--
--

-- Table A-17. Initial survival of invertebrate larvae 

### from Big Bend Screenwash Station, day and night sampling, May 1985. (1.00 = 100% survival). INITIAL SURVIVAL DAY NIGHT No. No. Taxa Organisms Tested 

## - X Organi 

### sms S.D. Tested 

### - X S.D. 

### ZOEA Penaeidae Caridea Portunidae Paguridae Xanthiidae Pinnotheridae Grapsidae MEGALOPS Xanthidae Graps idae JUVENILES Lo1 

### 1iguncul

### a

brevis



## - 323 0.87 0.27 0.89 0.28

20 1 

### .oo 0.00 1538 0.98 0.04 0.95 0.18 2459 0.86 0.32 0.80 0.32

113 0.99 0.04 1.00 0.00

8 1 

### .oo 1.00 0.67 0.58 37 0.83 0.41 .0.50 0.50

- X = Mean survival (percent: multiply by 100). S.D. = Standard deviation.

### -- -- -- 

### -- -- 

### --

-- --

-- 

### -- -- -- --

-- -- -- Table 

### A-18. Initial 

### survival of invertebrate larvae from Big Bend Screenwash Station, day and night sampling, 

### June 1985. (1.00 = 100% survival).


### DAY

. No. Organi 

### sms 

## - Taxa Tested X 

### ZOEA Penaeidae Caridea 83 0.78 Portunidae Paguridae 1 1

## .oo Xanthiidae 1031 0.95 Pinnotheridae 2655 0.99 Grapsidae 168 1.00 MEGALOPS Xanthi dae 1 1

## .oo Graps idae 7 1

## .oo
JUVENILES Lo1 1iquncul a

brevis



## - INITIAL SURVIVAL 

### N

### IGHT 

### No. Organisms


## - S.D. Tested X S.D.

0.37 37 0.62 0.46

0.00 

### 0.16 343 0-82 0.33 0.02 658 0.79 0.34 0.00

0.00 1 0.00 0.00 0.00 1 1.00 0.00



### - X = Mean survival (percent: multiply by 100). 

### S.D. = Standard deviation.

I

### --

-- --

-- 

### --

-- 

### --
--



### -- 

## -- --

-- 

### -- Table A-19. Initial survival of invertebrate larvae from Big Bend Screenwash Station, day and night sampling, July 1985. (1.00 = 100% survival). INITIAL SURVIVAL DAY 

### NIGHT No. No. Taxa Organisms Tested 

## - X S.D. Organisms Tested 

## - X 

### S.D. ZOEA Penaeidae Caridea Portunidae Paguridae Xanthiidae Pinnotheridae Grapsidae MEGALOPS Xanthidae Grapsidae JUVENILES Lo1 

### 1iguncula

brevis



## - 1 1-00 0.00 99 0.49 0.44 54 0.35 0.39

3 0.67 0.58 

### 2 1-00 0.00 725 0.90 0.16 169 0.74 0.36 5402 0.85 0.27 1184 0.73 0.34 59 0.85 0.33 35 0.75 0.50

1 1 

### .oo 0.00 1 1 

### .oo 0.00 4 1.00 0.00 1 0.00 0.00

- X = Mean survival (percent: multiply by 100). S.D. = Standard deviation.

### -- 

### -- 

### -- -- 

### -- --

-- 

### -- 

### --

-- -- -- -- -- Table A-20. 

### Initial survival of 

### invertebrate larvae from Big Bend Screenwash Station, day and night sampling, August 1985. (1.00 = 100% 

### survival). 

### INITIAL SURVIVAL DAY NIGHT No. 

### No. Taxa Organ

### i sms Tested 

### - X 

### S.D. Organisms Tested 

## - X 

### S.D. 

### ZOEA Penaeidae Caridea 47 0.43 0.43 79 0.62 0.42 

### Portunidae 1 1 

### .oo 0.00 

### Paguridae 4 1.00 0.00 2 1.00 0.00 

### Xanthiidae 957 0.97 0.07 428 0.95 0.07 

### Pinnotheridae 3916 0.91 0.24 2615 0.81 0.35 

### Grapsidae 15 0.95 0.11 26 0.93 0.19


### MEGALOPS Xanthidae 1 0.00 0.OCI 

### Grapsidae 45 1.00 0.00 33 0.57 0.49
JUVENILES 

### Lolliguncula
--.



### brevis

.. ­



## - = 

### Mean survival (percent: multiply by 100).

- S.D. = 

### Standard deviation. X

### -- -- 

## -- -- -- -- 

### --



### -- -- 

### -- Table A-21. Initial survival of invertebrate larvae 

### from Big Bend Screenwash Station, day and night sampling, September 1985. (1.00 = 100% survival).

Taxa ZOEA Penaeidae Caridea Portunidae Pag

### uridae Xanthiidae Pinnotheridae Grapsidae

Grapsidae JUVENILES Lo1 1iguncula

brevi

### s



## - INIT

### IAL SURV

### IVAL 

### DAY NIGHT No. No. Organisms 

### - Organisms


## -

Tested X S.D. Tested X S.D. 3 1.00 0.00 

### 2 1-00 0.00 5 0.75 0.35 9 1 

### .oo 0.00

4 0.50 0.71 1 1

### .oo 0.00 52 1 0.94 0.17 371 0.98 0.05 6303 0.91 0.21 386 1 0.99 0.02 18 1.00 0.00 19 1 

### .oo 0.00

1 1

### .oo 0.00

.. . X = Mean survival (percent: multiply by 100).


### - S.D. = Standard deviation.

c

### -- -- -- 

## --

--

-- -- -- -- --

-- 

### -- -- 

### -- --



### -- 

### -- -- -- -- -- -- Table A-22. Latent survival of hatched fish larvae from Big Bend Screenwash Station for 24 and 48 hours, day and night sampling, March 1985. (1.00 = 100% survival). 

# i i Taxa 

### Cl upeidae Engraulidae Atherinidae Sparidae Lagodon

rhomboides Sciaenidae Bairdiel 1a



### chry 

### soura Pogoni

### as

cromi

### s Cynoscion

nebul

### osus

B1enniidae Gobiidae



### - LATENT SURVIVAL 

### DAY 

### NIGHT No. 

### No. Organisms 24 hrs 48 

### hrs Organisms 24 

### hrs 48 hrs

Tested TI S.D. ST S.D. Tested 

### X S.D. TT 

### S.D. 3 1.00 1.00 9 1.00 1.00 147

2 0.75 0.35 0.75 0.35 142 0.96 0.07 0.85 0.18 168 1.00 0.00 0.92 0.09

3 1-00 0.00 1.00 0.00

4 0.75 0.35 0.75 0.35 3 0.75 0.35 0.75 0.35 1 1.00 1.00

- X = Mean survival (percent: mu1ti ply by 100). S.D. .= Standard deviation.

### -- -- 

### -- -- 

### --

-- -- -- -- --

-- -- 

### -- -- 

### -- i i

? E T Table A-23. Latent survival of hatched fish larvae ,from Big Bend Screenwash Station for 24 and 48 

### hours, day and night sampling, April 1985. (1.00 = 100% survival). 

### LATENT SURVIVAL DAY NIGHT No. No. Organisms 24 hrs 48 hrs Organisms 24 

### hrs 48 hrs Taxa Tested 

### 5? S.D. iT S.D. Tested 

### Y S.D. 

### X S.D. 

### Clupeidae 5 0.00 0.00 0.00 0.00 Engraulidae 225 0.94 0.15 0.88 0.21 251 0.91 0.25 0.84 0.27 Atherinidae 1 0.00 0.00 0.00 0.00
Lagodon

rhornboides Sciaenidae 279 0.77 0.26 0.73 0.29 178 0.83 0.32 0.72 0.33 Bairdi

### el 

### 1a



### chrysoura 3 1.00 0.00 1.00 0.00 Poqonias

crorni 

### s 1 1.00 0.00 1.00 0.00 3 1.00 0.00 1.00 0.00

Gobiidae 1 0.00 0.00 0.00 0.00 7

. 0.62 0.47 0.6.2 0.47 Soleidae 67 0.98 0.03 1.00 0.00 33 1.00 0.00 0.96 0.05



### -

- X = Mean survival (percent: multiply by 100). 

### S.D. = Standard deviation.

### -- -- -- -- 

## --

-- 

### -- 

### -- -- 

### -- 

## -- -- 

### -- -- --



### -- -- -- -- 

### -- Table A-24. Latent survival of hatched fish larvae from Big Bend Screenwash Station for 24 and 48 hours, day and night sampling, May 1985. (1.00 = 100% survival). LATENT SURVIVAL DAY 

### NIGHT No. No. Organisms 24 

### hrs 48 

### hrs Organisms 24 

### hrs 48 hrs Taxa Tested 

### F Tested 

### Y X I 

## 1 

# 1 

# I I
1 a I

Y 

# 4

F i I
z ii 

# 1 f t 

# 1 t ! 

# 1 i TT S.D. S.D. C1 upeidae Engraulidae 197 0.69 0.48 0.60 0.49

Sciaenidae 185 0.69 0.40 0.46 0.41

Cynoscion

nebul

### osus 1 0.00 0.00 0.00 0.00 Cynoscion

arenari

### us 7 0.83 0.24 0.67 0.47 

### Lei ostornus

xanthurus

Soleidae 87 0.92 0.19 0.88 0.31



### -

- X = Mean survival (percent: multiply by 100). S.D. = Standard deviation. S.D. S.D. 2 1.00 0.00 0.00 0.00 541 0.90 0.20 0.83 0.23

133 0.80 0.35 0.55 0.35 2 0.50 0.71 0.50 0.7:i.

9 1.00 0.00 1.00 0.00

-. ­ 2 1.00 0.00 1.00 0.00 3. 0.67 0.58 0.67 0.58 9 1.00 0.00 1.00 0.00

i t i

### -- -- -- --


### -- -- 

### -- --

-- 

### -- -- 

### -- --

-- -- -- 

### -- -- 

### -- -- -- 

## -- -- Table A-25. Latent 

### survival of hatched fish larvae from Big Bend Screenwash Station for 24 and 48 hours, day and night sampling, June 1985. (1.00 = 100% survival). 

### 4 LATENT SURVIVAL 

### DAY NIGHT No. ' No. Organisms 24 hrs 48 hrs Organisms 24 hrs 48 hrs Taxa Tested j? 

### S.D. TT S.D. Tested 

### F S.D. 'ii S.D. 

# f C1upeidae 

### 1 0.00 0.00 0.00 0.00 

## -_. Engraul idae 90 0.78 0.44 0.62 0.47 

### -_ 

# 1

Sciaenidae 101 0.75 0.38 0.44 0.47 119 0.84 0.30 0.77 0.42
Pogonias

cromi

### s Cynoscion

nebul osus Cynoscion

arenari

### us Leiostomus

xanthurus Blenni idae 

### 1 0.00 0.00 0.00 0.00 Gobiidae 1

- 1.00 0.00 1.00 0.00 Soleidae 135 0.88 0.35 0.83 0.36 6 1.00 0.00 1.00 0.00



## -

- X = Mean survival (percent: multiply by 100). S.D. = Standard deviation.

t

## -- -- -- 

## -- --

-- -- -- -- 

## -- -- -- -- 

### -- --



### -- -- -- -- 

### -- Table A-26. 

### Latent survival of hatched fish larvae from Big Bend Screenwash Station 

### for 24 

### and 48 

### hours, day and night sampling, July 1985. (1.00 

### 100% 

### survival). =


### LATENT 

### SURVIVAL 

### DAY No. Organisms 24 

### hrs 48 

### hrs

Tested 'iT 

### S.D. 

### P S.D. 7 1.00 0.00 

### 0.66 0.00

113 0.60 0.52 0.57 0.49

236 1.00 

### 0.00 0.92 0.29 

### NIGHT 24 

### hrs 48 

### hrs S.D. 32 S.D. 

### 0.00 0.00 

### 0.00 

### No. Organisms

Tested 1 

### Taxa C1upeidae Engraul i

### dae Atherinidae Sparidae Lagodon

rnomboides Sciaenidae Bairdiel 

### 1a

chrysoura Pogonias

cromi s 

### Cynosci on

nebul osus Cynosci on



### arenari us 

### Leiostomus

xanthurus Blenniidae Gobiidae

. 

### Soleidae



### - 1 

### 2

105

9 0.00 

### 1.00 0.00 1.00 0.00



### 0.86 0.35 0.69 0.39



### 0.72 0.26 0.72 0.26

- X = 

### Mean survival (percent: multiply by 100). S.'D. = 

### Standard deviation.

### -- 

### -- -- -- -- -- 

## -- -- -- --

-- -- 

### -- -- -- Table A-27. 

### Latent survival of hatched fish larvae from Big Bend Screenwash Station for 24 

### and 48 

### hours, day and night sampling, August 1985. (1.00 = 

### 100% 

### survival).
LATENT SURVIVAL DAY 

### NIGHT No. No. 

### Organisms TT 24 

### hrs 

### -48 

### hrs Organisms 24 

### hrs


## -48 

### hrs Taxa Tested S.D. x S.D. 

### Tested K S.D. x S.D. 

# 1 C1upeidae Engraul idae 191 0.89 

### 0.33 0.76 0.43 1 0.00 0.00 0.00 0.00 

# 1 1 f Sciaenidae 43 0.62 0.51 0.45 0.41 61 0.77 0.34 0.40 0.45 

### P 

### Bairdiel 

### 1 a 

# i ch

### ry

### so

### u

### ra 

### Pogoni as a t 

### cromi s 

### T Cynosci on nebul

### osus 

# f Cynoscion arenarius 

# f 

# f
5 1.00 0.00 0.67 

### 0.58 

# I Soleidae 252 0..95 0.16 0.94 0.16 4 1.00 0.00 1.00 0.00 I 

## j
- X - = 

### Mean survival (percent: multiply by 100). S.D. = 

### Standard deviation.

* 

# i

### -- 

### -- -- 

### -- 

### -- 

## -- -- -- 

### -- 

### --

-- -- 

### -- 

### -- -- -- 

### -- -- 

### -- --
-- 

## -- -- -- --


## -- -- 

## -- -- -- Table A-28. 

### Latent survival of hatched fish larvae from Big Bend Screenwash Station for 24 

### and 48 

### hours, day and night sampling, September 1985. 

### (1.00 = 

### 100% 

### survival).

Taxa C1

### upeidae Engraul idae Atherinidae Sparidae Laqodon

rhomboides Sciaenidae Bairdiel 1

### a

c

### hrysoura Poqonias

cromi s 

### Cynoscion

nebulosus Cynoscion

arenarius 

### Leiostomus

xanthurus 

### B1 enni idae Gobi idae Soleidae



### - LATENT SURVIVAL DAY NIGHT No. No. Organisms 24 

### hrs 48 

### hrs Organisms 24 

### hrs 48 

### hrs

Tested 5? 

### S.D. F 

### S.D. Tested 5? 

### S.D. K 

### S.D.

150 

### 0.82 0.38 0.68 0.43 

### 75 0.65 

### 0.43 

### 0.57 0.40

4 0.33 0.58 0.00 0.00 

### 69 0.34 0.42 0.25 0.43

.. . 

### 1 1.00 0.00 1.00 0.00 

### 21 0.41 0.43 0.31 0.43 100 1.00 0.00 

### 1.00 0.00 12 1.00 0.00 1.00 0.00

X = 

### Mean survival (percent: multiply by 

### 100).

- S.D. = 

### Standard deviation.

### -- -- -- -- 

### --
-- 

## --
-- -- -- 

### -- 

### --

-- 

### -- -- -- 

### --
-- -- -- 

### -- 

## --
-- -- -- -- -- I



### Table A-29.



### Taxa C1upeidae Anchoa mitchilli Atherinidae Sparidae Lasodon rhomboides Sciaenidae Bairdiella chrysoura Poqonias cromis Cvnoscion nebulosus Cvnoscion arenarius Leiostomus xanthurus B1

### enni idae Gobi idae Soleidae



## - Summary of 

### the latent survival of fish larvae from Big Bend Screenwash Station for 24 

### and 48 

### hours, March 1985. 

### (1.00 = 

### 100% 

### survival).
24 

### hrs 48 

### hrs 

### No.

- - 

### Tested X S.D. X S.D.

1 1 

### .oo 1.00

3 1 

### .oo 0.00 1 

### .oo 0.00



### x = 

### Mean survival (percent: multiply by 100) 

### S.D. = 

### Standard deviation i

### -- 

## --

--

--



## -- -- --

--
--
--

--



### --

--
-- --


### --

--



### --



### -- --

--
--


### --

--

--

--

-- 

### Table A-30.

Taxa Cl upeidae Anchoa mitchilli Atherinidae Sparidae Laqodon rhomboides Sciaenidae Bairdiella chrvsoura Poqonias cromis Cvnoscion nebulosus 

### Cvnosci

### on arenarius 

### Leiostomus xanthurus B1 

### enni idae Gobi idae Soleidae Anchoa sp. Summary of 

### the latent survival of fish larvae from Big Bend Screenwash Station for 24 

### and 48 

### hours, April 1985. (1.00 = 100% 

### survival).
24 

### hrs 48 

### hrs No.


### - Tested X 0.00 0.80

1

## .oo 0.88 1 

### .oo 1 

### .oo

0.50

5 0.50 5 0.66

1 1.00 - 

### S.D. X S.D.
0.06 0.28 0.79 0.27

1 

### .oo 0.12 0.83 0.17 0.00 1.00 0.00 0.00 1.00 0.00

0.71 0.50 0.71

0.71 0.50 0.71 0.58 0.66 0.58

- 1.00

TT = 

### Mean survival (percent: multiply by 100) 

### S.D. = 

### Standard deviation



# I/

### -- -- -- -- 

## --

-- -- -- -- 

## -- -- -- -- -- 

### --
-- -- -- -- 

### --

-- -- -- 

### -- --
-- -- -- -- --


### -- 

### --

-- -- 

### -- -- 

### --
-- -- -- 

### -- 

### -- Table A-31.



### Taxa C1 upeidae Anchoa mitchilli Atherinidae Sparidae Laqodon rhomboides Sciaenidae Bairdiell a 

### chrvsoura Poqonias cromis Cvnoscion nebulosus Cvnoscion arenarius Leiostomus xanthurus Bl

### enni i

### dae Gobi idae Soleidae Summary of 

### the latent survival of fish larvae from Big Bend Screenwash Station for 24 

### and 48 

### hours, May 1985. 

### (1.00 = 

### 100% 

### survival).


### 24 hrs 48 

### hrs No.


### ­ - 

### Tested X 

### S.D. X 

### S.D.

8 1.00 

### 0.00 1 

### .oo 0.00

66 

### 0.80 0.22 0.53 0.36

1 0.00 0.90 16 0.70 0.41 0.60 0.42

4 0.50 

### 0.71 0.50 0.71 4 

### 1.00 0.00 ._ 1

### ..oo 0.00



### X = 

### Mean survival (percent: multiply by 100) 

### S.D. = 

### Standard deviation

f iTable A-32.

Taxa Summary of 

### the latent survival of fish larvae from Big Bend Screenwash Station for 24 and 48 hours, 

### June 1985. (1.00 = 100% survival).

. 24 

### hrs 48 hrs No.


### - - Tested X S.D. X S.D. Clupeidae Anchoa rnitchilli 

### Atherinidae Sparidae Laqodon rhornboides Sciaenidae Bairdiella chrvsoura Poqonias crornis Cvnoscion nebulosus Cvnoscion arenarius Leiostomus xanthurus B1 enniidae Gobi idae Soleidae 8 1.00

ST = Mean survival (percent: multiply by 100) 

### S.D. = Standard deviation

### --

-- -- 

### --

-- 

### --

--

--

--

-- 

### -- Table A-33.

Taxa Clupeidae Anchoa mitchilli Atherinidae Sparidae Lasodon rhomboides Sciaenidae Bairdiella chrvsoura Poaonias cromi s Cynoscion nebulosus Cvnoscion arenarius Leiostomus xanthurus Blenni idae Gobiidae Soleidae



### - Summary of the latent survival of fish larvae from Big Bend Screenwash Station for 24 and 48 hours, July 1985. (1.00 = 100% survival).
24 

### hrs 48 

### hrs 

### No.

- 

### - Tested X 

### S.D. X S.D.

5 1 

### .oo 0.00 1

### .oo

30 0.93 0.12 0.87

9 0.89

1-00 0.00 0 ..87 0.25

x = Mean survival (percent: multiply by 100) S.D. = Standard deviation

### --

--


### --

--

--


### --

--


### -- Table A-34. Summary 

### of 

### the latent survival of fish larvae from
/ Big Bend Screenwash Station for 24 and 48 hours, August 1985. (1.00 = 100% survival).



### No. Taxa Tested 

### C1upeidae Anchoa mitchilli Atherinidae Sparidae Laqodon rhomboides Sciaenidae Bairdiel 

### 1 a chrvsoura Poqonias cromis Cvnoscion nebulosus Cvnoscion arenarius Leiostornus xanthurus 

### B1enniidae Gobi idae Soleidae 3



## - x = Mean survival (p rcent: multipl S.D. = Standard deviation 24 

### hrs 48 

### hrs



### - - X S.D. X S.D.

0.00

1 

### .oo 1-00

0.51

1-00 1 

### .oo
by 100)

### -- 

## -- -- 

### --

-- 

### --
-- --
-- 

### --

-- 

### --
-- --
-- --

-- 

### -- --

-- E



### -- -- -- -- 1 I 

## \ Table A-35. 

### 1 

## I i
Taxa C1upeidae Anchoa mitchilli 

### Atherinidae Sparidae Laaodon rhomboides Sciaenidae
I Bairdiella chrvsoura Poaonias cromis Cvnoscion nebulosus



# - * a. Cynoscion arenarius Leiostomus xanthurus �31 enniidae Gobi idae Soleidae



## - x = Mean survival (percent: 

### S.D. = Standard deviation Summary of 

### the latent survival of fish larvae from Big Bend Screenwash Station for 24 and 48 hours, September 

### 1985. (1.00 = 100% survival).
24 

### hrs 48 hrs No.


### - - Tested X S.D. X S.D.

10 0.75 0.50 

### 0.66

32 0.43

53 0.49 0.42

17 0.50 0.45 0.37 0.49 10 0.70 0.45 0,70 0.45

mu1 ti ply by 100)

## -- -- -- 

## -- -- -- 

### -- -- 

### -- 

## -- Table A-36. 

### Latent survival of 

### invertebrate larvae from 

### Big Bend Screenwash Station for 24 

### and 48 

### hours, day and night sampling, March 1985. ' (1.00 = 100% 

### survival).
LATENT SURVIVAL DAY N

### IGHT No. No. 

### Organisms 24 

### hrs 48 

### hrs Organisms 24 

### hrs


## - 48 

### hr5 Taxa Tested K 

### S.D. r? 

### S.D. Tested 7 

### S.D. x S.9,

.-_ 

### ZOEA Penae i

### dae Caridea 141 0.80 0.26 0.72 0.30 72 0.88 0.18 0.81 0.24 

### Portunidae 1 1.00 0.00 0.00 0.00 2 0.75 0.35 0.25 0.35 

### Paguridae 16 1.00 0.00 0.69 0.46 16 0.59 0.26 0.59 0.26 

### -Xanthiidae 109 0.77 0.32 0.66 0.35 135 0.79 0.34 0.74 0.37



### Pinnotheridae 42 0.77 0.37 0.58 0.40 46 0.74 0.32 0.61 0.39 

### Grapsidae 1 0.88 0.25 0.88 0.25 1 1.00 0.00 0.75 0.00


### MEGALOPS Xanthiidae Grapsidae
JUVENILES Lo1

### 1 iguncula

brevis 2 0.00 0.00 0.00 0.00 2 0.00 "0.00 0.00 0.00



## -

- X = 

### Mean survival (percent: multiply by 100). S.D. = 

### Standard deviation.

## -- -- -- -- 

### --

-- 

# 1 Table A-37. 

### Latent survival of 

### invertebrate larvae from Big Bend Screenwash Station for 24 

### and 48 

### hours, day and night sampling, April 1985. 

### (1.00 = 100% 

### survival). 

# 1 1 LATENT SURVIVAL DAY 

### NIGHT 

# 7 ' NO. 

### No. Organisms S.D. 

## - 48 

### hrs Organisms 24 

### hrs 48 

### hrs Taxa Tested 

### X 24 

### hrs x S.D. 

### Tested TT S.D. -E S.D.



### Caridea 231 0.81 0.40 0.59 0.31 88 0.45 0.40 0.78 0.32 

### Portunidae 1 1.00 0.00 1.00 0.00 

### Paguridae 15 0.30 0.23 0.75 0.29 3 0.67 0.58 0.67 0.58 

### Xanthiidae 159 0.75 0.31 0.63 0.38 125 0.90 0.26 0.89 0.26 

### Pinnotheridae 79 0.38 0.29 0.71 0.27 98 0.90 0.16 0.79 0.20 

### Grapsi

### dae 2 1-00 

### 0.00 1.00 0.00 4 1.00 0.00 0.75 0.50


### MEGALOPS Xanthiidae 18 1.00 0.00 1.00 0.00 3 1.00 0.00 1.00 0.00 

### Grapsidae 52 -0.99 0.03 0.97 0.06 24 1.00 0.00 1.00 0.00


### JUVENILES Lo1 1 

### iguncula

brevis 13 0.22 

### 0.40 0.22 0.40 13 0.80 0.20 0.00 0.00



## - X = 

### Mean survival- (percent: multiply by 100).

- 

### S.D. = 

### Standard deviation.

### -- 

## -- -- 

### -- -- 

### -- 

## -- -- -- --



### -- -- 

## -- -- -- Table A-38. 

### Latent survival of 

### invertebrate larvae from Big Bend Screenwash Station for 24 

### and 48 

### hours, day and night sampling, May 1985. (1.00 = 100% 

### survival).
LATENT SURVIVAL

Taxa ZOEA Penaeidae Caridea Portunidae Paguri

### dae Xanthiidae Pinnotheridae Grapsidae
MEGALOPS Xanthiidae Grapsidae
JUVENILES Lo1 

### 1 i

### guncul a

brevis



# - DAY 

### No. Organisms 24 

### hrs 48 

### hrs Tested 

### P ST S.D. S.D.

198 0.70 0.28 0.59 0.28 1 1.00 0.00 1.00 0.00 6 0.60 0.54 0.60 0.54 242 0.86 0.21 0.75 0.30

76 0.87 0.16 0.76 0.26 14 0.73 0.48 0.73 0.48

53 1.00 0.00 0.99 0.02 117 0.85 0.23 0.84 0.23

5 0.00 0.00 0.00 0.00 

### NIGHT No. Organisms 24 

### hrs


## - 48 

### hrs

Tested 

### W S.D. 

### x S.D.

146 0.74 0.34 0.68 0.35

157 0.87 0.30 0.78 0.38 126 0.86 0.19 0.71 0.29

34 1.00 0.00 0.87 0.23

45 0.97 0.05 0.86 0.21 150 0.96 0.05 0.91 0.12



### 3 0.00 

### -o..oo 0.00 0.00

- X = 

### Mean survival (percent: multiply by 100). S.D. = 

### Standard deviation.

### -- 

### -- -- 

## -- -- 

## -- -- 

### -- 

## -- --

-- -- -- -- --


### -- -- -- -- 

### -- 

# 3
I t 

# 7 

# 1 1

i 

# 1 Table A-39. Latent survival of 

### invertebrate larvae from Big Bend Screenwash Station for 24 

### and 48 

### hours, day and night sampling, June 1985. (1.00 = 100% 

### survival).
LATENT SURVIVAL 

### DAY NIGHT 

### No. No. Organisms 24 

### hrs 

## -48 

### hrs Organisms 24 

### hrs 

## -48 

### hrs Taxa Tested Tc 

### S.D. 

### x S.D. 

### Tested 7? 

### S.D. x 

### S,D. ZOEA Penaeidae Caridea 107 0.80 0.26 0.68 0.30 143 0.87 0.17 0.81 0.26 

### Portunidae 1 1.00 0.00 1.00 0.00 

### Paguridae 2 1.00 

### 0.00 1.00 0.00 

### Xanthi idae 135 0.92 0.22 0.84 0.30 139 

### 0.93 0.21 0.90 0.24 

### Pinnotheridae 163 0.95 0.11 0.76 0.30 233 0.86 0.15 0.80 0.19 

### Grapsidae 19 1-00 0.00 1.00 0.00 18 0.97 0.04 0.97 0.04 

### NEGALOPS Xanthiidae 3 1.00 

### 0.00 1.00 0.00 4 0.88 0.18 0.88 0.18 

### Grapsidae 60 

### 1.00 0.00 

### 0.97 0.06 27 1.00 0.00 0.99 0.02


### JUVENILES Lo7 1 

### i

### guncul a

brevis 1 0.00 0.00 0.00 0.00 2 0.00 0.00 0.00 0.00



### -

- X = 

### Mean survival (percent: multiply by 100). 

### S.D. = 

### Standard deviation.

## -- -- 

## -- -- -- Table A-40. 

### L t t urvival of 

### invertebrate larvae from Big Bend Screenwash Station 

### for 24 

### and 48 

### hours, day and night sampling, July 1985. 

### (1.00 = 100% 

### survival).
LATENT SURVIVAL DAY 

### NIGHT No. , No. 

### Organisms 24 

### hrs



### - 48 

### hrs Organisms 24 

### hrs 

## - 48 

### hrs Taxa Tested 

### W S.D. x 

### S.D. Tested 

### R S.D. x S,D.
23 1.00 0.00 1.00 0.00 

### Caridea 179 0.72 0.35 0.49 0.38 93 0.71 0.38 0.60 0.41 1 1.00 0.00 1.00 0.00 

### Paguridae 11 1.00 

### 0.00 0.69 0.41 5 1.00 0.00 1.00 0.00 

### Xanthiidae 70 0.94 0.20 0.81 0.33 66 0.89 0.26 0.82 0.31 

### Pinnotheridae 366 0.88 0.15 0.68 0.26 516 0.85 0.13 0.77 0.18 

### Grapsi

### dae 10 0.91 0.15 0.70 0.40 25 0.91 0.19 0.91 0.19


### MEGALOPS Xanthiidae 2 

### 1.00 0.00 1.00 0.00 4 1.00 0.00 1.00 0.00 

### Grapsidae 10 -1.00 

### 0.00 1.00 0.00 54 0.99 0.02 0.87 0.25


### JUVENILES Lo1 

### 1 iguncula

brevis



## -

- X = 

### Mean survival (percent: multiply by 100). 

### S.D. = 

### Standard deviation.

### Table A-41. 

### Latent survival of 

### invertebrate larvae from Big Bend Screenwash Station for 24 

### and 48 

### hours, day and night sampling, August 1985. 

### (1.00 = 

### 100% 

### survival).
LATENT SURVIVAL DAY

. 

### NIGHT No

### - - 

### No. Organisms 24 

### hrs 

### - 48 

### hrs Organisms 24 

### hrs


## - 48 

### hrs Taxa Tested Y 

### S.D. x S.D. 

### Tested 51 S.D. 

### x 

### S.D. ZOEA 

### Penaeidae 1 1.00 0.00 1.00 0.00 12 0.92 0.14 0.92 0.14 

### Caridea 202 0.84 0.18 0.54 0.33 176 0.84 0.18 0.75 0.22 

### Portunidae 12 1.00 0.00 1.00 0.00 4 0.90 0.14 0.90 0.14 

### Paguri

### dae 5 0.38 0.48 0.38 0.48 3 1-00 0.00 1.00 0.00 

### Xanthiidae 126 0.87 0.23 0.74 0.30 64 0.94 0.20 0.89 0.28 

### Pinnotheridae 284 0.83 0.18 0.60 0.27 472 0.88 0.08 0.67 0.16

1 

### Grapsidae 6 0.70 0.45 0.70 0.45 18 1-00 0.00 1.00 0.00

I 

### MEGALOPS Xanthi idae 3 1-00 0.00 1.00 0.00 7 1-00 0.00 1.00 0.00

I 

### Grapsidae 200 0.99 0.02 0.97 0.08 142 0.99 0.02 0.99 0.02

E 

### JUVENILES i 

### Lo1

### 1 iguncula brevis 

### i t 

# ­

- X = 

### Mean survival (percent: multiply by 100). S.D. = 

### Standard deviation. 

# 1 i

f

### --



## -- Table A-42. 

### Latent survival of invertebrate larvae from Big Bend Screenwash Station for 24 

### and 48 

### hours, day and night sampling, September 1985. (1.00 = 100% 

### survival).
LATENT SURVIVAL

Taxa ZOEA 

### Penaeidae Caridea Portunidae Paguridae Xanthiidae Pi 

### nnotheridae Grapsidae
MEGALOPS Xanthiidae Grapsidae
JUVENILES Lo1 

### 1 i

### guncula

brevis



## - DAY No. 

### Organisms 24 

### hrs 48 

### hrs

Tested ST S.D. Z S.D.

266 6 

### 11 123 354

10

37 194 0.85 0.21 0.72 0.29 1.00 0.00 1.00 0.00 0.75 0.42 0.75 0.42 0.92 0.18 0.85 0.29 0.82 0.18 0.67 0.24 0.80 0.45 0.80 0.45

0.99 0.02 0.96 0.09 0.96 0.05 0.96 0.05 

### NIGHT 

### No. Organisms 24 

### hrs 48 

### hrs


## - Tested K S.D. x 

### S.G.

16 0.79 0.37 0.79 0.37 187 0.87 0.22 0.75 0.32 

### 5 1.00 0.00 1.00 

### O.QO 4 0.83 0.24 0.83 0.24 123 0.91 0.22 0.85 0.30 644 0.86 0.09 0.76 0.13

14 0.83 0.41 0.83 0.41

36 0.98 0.06 0.92 0.18 200 0.98 0.03 0.95 0.06

- X = 

### Mean survival (percent: multiply by 100). S.D. = 

### Standard deviation.

### APPENDIX B 

### FMS SURVIVABILITY STUDIES DATA ORGAN

### ISM 

### RETURN DI

### S

### CHARGE STAT1

### ON

### -- -- 

### --

-- 

### -- --

-- 

## -- --

-- 

### -- -- 

### Table 8-1. 

### Initial survival and hatchability of fish eggs, Organism Return Discharge Station, Big Bend Power Plant, for March 1985, 

### day and night sampling. (1.00 = 100% 

### survival and hatchability).
MARCH DAY 

### - No. Initial Organisms


## - Survival 

### S.D. Total


## - Hatchability Tested X No. X S.D.


### CLUPE

### IDAE 4 0.58 0.11 

### ENGRAULIDAE 229 0.24 0.25 64 0.83 0.32 

### SCIAENIDAE 367 0.36 0.35 128 0.75 0.31 

### SOL

### EIDAE 1 

### 0.00 0.00



### NIGHT



# - No. Initial Organi 

### sms 

## - SurviVal S.D. Total


## - Hatchability Tested X 

### No. X S.D.


### CLUPEIDAE 5 

### 0.40 0.00 

### ENGRAULIDAE 160 0.13 0.13 58 

### .o 

## .91 0 -35 

### SCIAENIDAE 2079 0.16 0.15 123 0.63 0.23 

### SOLEIDAE

. 1 0.00 0.00



## -

- X = 

### Mean survival (percent: multiply by 100). S.D. = 

### Standard deviation.

### -- -- -- 

### -- -- 

### --



### --

-- -- 

### -- 

## -- -- --



### -- 

# 1 Table B-2. 

### Initial survival and hatchability of fish eggs, Organism Return 

## t 

## I Discharge Station, Big Bend Power Plant, for April 1985, 

### day and night sampling. (1.00 = 

### 100% 

### survival and hatchability). 

# 1 APRIL DAY


### - No. Initial Organi 

### sms 

## - SurviVal Total

Tested X 

### S.D. No. 

# 1 C

### LUPEIDAE 

# I ENGRAULIDAE 1143 0.29 0.28 239 

### SCIAENIDAE 1212 0.31 0.23 277 

# f I 

### SOLEI

### DAE 1 1 

### .oo 0.00 55 

# t

b f 

### NIGHT No. Initi a1 Organisms 

## - Survivaf Total 

# i 2, 

### Tested X 

### S.D. No. 1 

### CLUPEIDAE 

### f 

## - Hatchability X 

### S.D.

0.90 0.28 0.85 0.21 1 

### .oo



## - Hatchability X 

### S.D.

-



### ENGRAULIDAE 209 0.08 0.12 150 -0-87 0.31 

# 1 t 

### SCIAENIDAE 5126 0.48 0.31 206 0.65 0.14 j 

### SOL

### EIDAE. 3 

### 0.83 0.29 42 1.00 ! 

# I -

- X = 

### Mean survival (percent: multiply by 100). 

### S.D. = 

### Standard deviation.

### -- -- 

### -- -- -- --



## --

-- -- 

### -- -- -- 

### --
-- Table 

### 8-3. Initial survival and hatchability of fish eggs, Organism Return Discharge Station, Big Bend Power Plant, for May 1985, 

### day and night sampling. (1.00 = 

### 100% 

### survival and hatchability).

DAY 

## - No. Initial Organi 

### sms 

## - SurviVal 

### S.D. Total Tested X 

### No.
i 

### CtUPEIDAE ENGRAULIDAE 418 0.57 0.34 194 

### SCIAENIDAE 985 0.43 

### 0.41 169 

### SOLEIDAE 3 1 

### .oo 0.00 110
I 

### i

I 

### NIGHT No. Initial Organisms

-. 

### SurviVal S.D. I 

### Tested X i 

## I E 

### CLUPEIDAE Hatchabi 

### 1 i

### ty 

# - X S.D.

0.97 0.39 0.97 0.38 0.99



### Hatchabi 

### 1 i 

### ty 

# - X 

### S.D.

-



### ENGRAULIDAE 44 

### 0.18 

### 0.40 5 - 

### 1 

### ..oo. 

# I SCIAENIDAE 2713 0.73 0.19 139 0.76 0.26 

# 1 I - X = 

### Mean survival (percent: multiply by 100).



### - S.D. = 

### Standard deviation. 

# 1 E c
i t 

### !