# Patent

## Title
Bacteriophage and antibacterial composition comprising the same

## Abstract

The present invention relates to a bacteriophage having a specific bactericidal activity against Salmonella, a composition for the prevention or treatment of infectious diseseases comprising the bacteriophage as an active ingredient, an antibiotic comprising the bacteriophage as an active ingredient, an animal feed or drinking water comprising the bacteriophage as an active ingredient, and a sanitizer or cleaner comprising the bacteriophage as an active ingredient. The bacteriophage of the present nvention has a specific bactericidal activity against Salmonella choleraesuis, Salmonella typhimurium, Salmonella derby, Salmonella infantis, or Salmonella newport with no influences on beneficial bacteria, as well as excellent acid- and heat-resistance and desiccation tolerance. Therefore, the bacteriophage can be used for the prevention or treatment of salmonellosis or salmonella food poisoning, which is an infection disease cuased by Salmonella choleraesuis, Salmonella typhimurium, Salmonella derby, Salmonella infantis, or Salmonella newport, and also widely used in animal feeds, drinking water for livestock, sanitizers, and cleaners.

## Background
CROSS-REFERENCES TO RELATED APPLICATIONS
This application is a U.S. national phase application of International PCT Patent Application No. PCT/KR2012/007555, which was filed on Sep. 20, 2012, which claims priority to Korean Patent Application No. 10-2011-0094648, filed Sep. 20, 2011. These applications are incorporated herein by reference in their entireties.
STATEMENT REGARDING SEQUENCE LISTING
The Sequence Listing associated with this application is provided in text format in lieu of a paper copy, and is hereby incorporated by reference into the specification. The name of the text file containing the Sequence Listing is HANO_025_00US_ST25.txt. The text file is 11 KB, was created on Mar. 18, 2014, and is being submitted electronically via EFS-Web.
TECHNICAL FIELD
The present invention relates to a novel bacteriophage and an antibacterial composition comprising the same.
BACKGROUND ART
Salmonella has an average genomic GC content of 50-52%, which is similar to that of Escherichia coli and Shigella. The genus Salmonella is a pathogenic microorganism that causes infections in livestock as well as in humans. Serological division has it that Salmonella enterica, a species of Salmonella bacterium, has a variety of serovars including Salmonella gallinarum, Salmonella pullorum, Salmonella typhimurium (ST), Salmonella enteritidis (SE), Salmonella typhi, Salmonella choleraesuis (SC), Salmonella derby (SD). Of them, choleraesuis and derby are swine-adapted pathogens, gallinarum and pullorum are fowl-adapted pathogens, typhimurium and enteritis are pathogenic for humans and animals, and typhi is a human-adapted pathogen, all of which cause illness in their respective species, resulting in tremendous damage to farmers and consumers (Zoobises Report; United Kingdom 2003).
Recently, implementation of HACCP (Hazard analysis and critical control points) has become a mandatory requirement for all slaughterhouses in Korea as of Jul. 1, 2003, because of the high risk of contamination in the course of manufacturing livestock products with salmonella, which causes direct damage to pigs, and meat hygiene and is found in the digestive tract of pigs (Jae-gil Yeh. Characterization and Counterplan of Salmonellosis in Pigs. Monthly Magazine of Pig Husbandry. 2004).
Paratyphoid, the acute or chronic infectious disease in the digestive tract of pig caused by salmonella infection, is characterized by gastroenteritis and septicaemia and mainly occurs during the fatting period. In particular, some of pathogenic bacteria that cause this disease can cause food poisoning in humans through meat ingestion, and thus it is a disease having major public health importance. A variety of types of salmonella bacteria can be pathogenic. Among them, Salmonella choleraesuis and Salmonella typhisuis known to cause hog cholera are the major causes of acute salmonella septicaemia. Acute enteritis occurs during the fattening period, and is accompanied by irregular appetite, severe watery diarrhea, high fever, loss of vitality, pneumonia, and nervous signs. Discoloration of the skin may occur in some severe cases. Salmonella typhimurium, Salmonella enteritidis, and Salmonella derby are the major causes of chronic enteritis.
Salmonellosis is caused by oral route through feed or water contaminated with salmonella, and thus these routes should be prevented. Contaminated feed, raw materials or water, or adult pigs carrying the pathogen can be major sources of infection. During the acute period of infection, pigs shed up to 106 Salmonella choleraesuis or 107 Salmonella typhimurium per gram of feces. However, many experimental infections reported successful disease reproduction with a dose of 108 to 1011 Salmonella. In an experiment injecting 103 Salmonella into pigs, the injected pigs showed no symptoms of the disease, but other pigs raised in the same pen showed typical clinical symptoms. These results indicate that a large amount of salmonella grow in naturally infected pigs, resulting in the infection of other pigs (Jung-Bok Lee. Control of the Recent Outbreaks of Porcine Salmonella and Proliferative Enteropathy. Korea Swine Association. 2009).
At present, severe viral infections such as Porcine Reproductive and Respiratory Syndrome (PRRS) and Porcine Circovirus (PCV2) have been causing tremendous economic losses to the swine industry in Korea, and thus disease management has been focused on these diseases. Since these bacterial diseases may cause tremendous damage comparable to that caused by viral diseases beginning with a ban on the use of in-feed antibiotics and an investigation of disease occurrence or disease management should be performed in advance (Jung-Bok Lee. Control of the Recent Outbreaks of Porcine Salmonella and Proliferative Enteropathy, Infectious Disease Laboratory, College of Veterinary Medicine, Konkuk University, Livestock Product Safety, 2010) (Robert W. Wills, Veterinary Microbiology, 1999). Meanwhile, bacteriophage, also called phage, is a specialized type of virus that infects only particular bacteria and controls the growth of bacteria, and can self-replicate only inside the host bacteria. After the discovery of bacteriophages, a great deal of faith was initially placed in their use for infectious-disease therapy. However, when broad spectrum antibiotics came into common use, bacteriophages were seen as unnecessary due to a specific target spectrum. Antibiotics or antimicrobial agents have been widely used for the treatment of infectious diseases caused by bacterial infection. Nevertheless, the misuse and overuse of antibiotics resulted in rising concerns about antibiotic resistance and the harmful effects of residual antibiotics in foods. However, the removal of current in-feed antibiotics might increase occurrence of bacterial diseases including salmonellosis that have been controlled by antibiotics, as expected in the experiment data or from other countries. Thus, there is an urgent need to establish a detailed practical guideline for salmonella management (Jung-Bok Lee. Control of the Recent Outbreaks of Porcine Salmonella and Proliferative Enteropathy, Infectious Disease Laboratory, College of Veterinary Medicine, Konkuk University, Livestock Product Safety. 2010).
These growing concerns have led to a resurgence of interest in bacteriophage. Seven bacteriophages for control of E. coli O157:H7 are disclosed in U.S. Pat. No. 6,485,902 (2002) and two bacteriophages for control of various microorganisms are disclosed in U.S. Pat. No. 6,942,858 (issued to Nymox in 2005). Many companies have been actively trying to develop various products using bacteriophages. EBI food system (Europe) developed a food additive for preventing food poisoning caused by Listeria monocytogenes, named Listerix-P100, which is the first bacteriophage product approved by the USFDA. A phage-based product, LMP-102 was also developed as a food additive against Listeria monocytogenes, approved as GRAS (Generally Regarded As Safe). In 2007, a phage-based wash produced by OmniLytics was developed to prevent E. coli 0157 contamination of beef during slaughter, approved by USDA's Food Safety and Inspection Service (FSIS). In Europe, Clostridium sporogenes phage NCIMB 30008 and Clostridium tyrobutiricum phage NCIMB 30008 were registered as feed preservative against Clostridium contamination of feed in 2003 and 2005, respectively. Such studies show that research into bacteriophages for the control of antibiotic-unsusceptible bacteria and contamination of livestock products by zoonotic pathogens is presently ongoing.
However, most of the phage biocontrol studies have focused on the control of E. coli, Listeria, and Clostridium. Salmonella is also a zoonotic pathogen, and damages due to this pathogen are not reduced. As mentioned above, since Salmonella exhibits multiple drug resistances, nationwide antimicrobial resistance surveillance has been conducted in Korea under the Enforcement Decree of the Act on the Prevention of Contagious Disease (Executive Order 16961), Enforcement Ordinance of the Act on the Prevention of Contagious Disease (Ministry of Health and Welfare's Order 179), and Organization of the National Institute of Health (Executive Order 17164). Accordingly, there is a need for the development of bacteriophages to control Salmonella. 
DISCLOSURE
Technical Problem
In order to overcome problems occurring upon the misuse or overuse of broad spectrum antibiotics, such as drug resistant bacteria and drug residues in foods, the present inventors isolated a bacteriophage from natural sources, in which the bacteriophage has a specific bactericidal activity against salmonella causing major diseases in livestock. As a result, they found that the bacteriophage has a specific bactericidal activity against Salmonella choleraesuis (SC), Salmonella typhimurium (ST), Salmonella derby (SD), Salmonella infantis (SI) and Salmonella newport (SN) with no influences on beneficial bacteria, in addition to showing excellent acid- and heat-resistance, as identified for the morphological, biochemical and genetic properties thereof. Further, they found that the bacteriophage can be applied to compositions for the prevention or treatment of Salmonella typhimurium-mediated diseases, such as livestock salmonellosis and Salmonella food poisoning, and to various products for the effective prevention and control of Salmonella bacteria proliferation, including livestock feed additives, drinking water for livestock, barn sanitizers, and cleaners for meat products, thereby completing the present invention.
Technical Solution
An object of the present invention is to provide a novel bacteriophage having a bactericidal activity against Salmonella choleraesuis. 
Another object of the present invention is to provide a composition for the prevention or treatment of infectious diseases caused by Salmonella choleraesuis, Salmonella typhimurium, Salmonella derby, Salmonella infantis or Salmonella newport, comprising the bacteriophage as an active ingredient.
Still another object of the present invention is to provide an antibiotic, comprising the bacteriophage as an active ingredient.
Still another object of the present invention is to provide an animal feed or drinking water comprising the bacteriophage as an active ingredient.
Still another object of the present invention is to provide a sanitizer or cleaner, comprising the bacteriophage as an active ingredient.
Still another object of the present invention is to provide a method for preventing or treating of infectious diseases caused by Salmonella choleraesuis, Salmonella typhimurium, Salmonella derby, Salmonella infantis or Salmonella newport, using the bacteriophage or the composition.
Advantageous Effects
The novel bacteriophage of the present invention has a specific bactericidal activity against Salmonella choleraesuis, Salmonella typhimurium, Salmonella derby, Salmonella infantis or Salmonella newport with no influences on beneficial bacteria, and excellent acid- and heat-resistance and desiccation tolerance. Therefore, the novel bacteriophage can be used for the prevention or treatment of salmonellosis or salmonella food poisoning, which is an infectious disease caused by Salmonella choleraesuis, Salmonella typhimurium, Salmonella derby, Salmonella infantis or Salmonella newport, and also widely used in animal feeds, drinking water for livestock, sanitizers, and cleaners.



DESCRIPTION OF DRAWINGS
FIG. 1 is an electron microscopy photograph of ΦCJ11, which belongs to the family Siphoviridae of morphotype B1, characterized by an isometric capsid and a long non-contractile tail;
FIG. 2 is a photograph showing the formation of ΦCJ11 plaques in a lawn of salmonella bacteria, in which (A,B;SC, C;SG, D;ST, E;SI, F,G;SD, H;SN), plaque formation was observed in lawns of SC, ST, SI, SD and SN, but not in lawns of SG;
FIG. 3 is the result of SDS-PAGE of the isolated bacteriophage ΦCJ11, in which the major proteins were detected at 33 kDa, 55 kDa and 69.5 kDa, and Precision plus protein standard (BIO-RAD) was used as a marker;
FIG. 4 is the result of PFGE of the isolated bacteriophage ΦCJ11, in which a total genome size of ΦCJ11 was approximately 140 kbp, and the CHEF DNA Size Standard Lambda Ladder (Bio-Rad) was used as a DNA size marker;
FIG. 5 is the result of PCR, performed using each primer set for the ΦCJ11 genomic DNA, in which A; a primer set of SEQ ID NOs. 5 and 6, B; a primer set of SEQ ID NOs. 7 and 8, C; a primer set of SEQ ID NOs. 9 and 10, and D; a primer set of SEQ ID NOs. 11 and 12, and all of A, B, C and D lanes had PCR products of approximately 1 kbp or more to 2 kbp or less;
FIG. 6 is the result of acid-resistance assay on the bacteriophage ΦCJ11, showing the number of surviving bacteriophage at pH 2.1, 2.5, 3.0, 3.5, 4.0, 5.5, 6.4, 6.9, 7.4, 8.0, 9.0, 9.8 and 11.0, in which the bacteriophage ΦCJ11 did not lose its activity until pH 5.5, but the bacteriophage ΦCJ11 showed reduced activity at pH 4 and pH 3.5, and completely lost its activity at pH 3.0 or lower, as compared to a control;
FIG. 7 is the result of heat-resistance assay on the bacteriophage ΦCJ11, showing the number of surviving bacteriophage at 37, 45, 53, 60, and 70° C. for 0, 10, 30, 60 and 120 minutes, in which the bacteriophage ΦCJ11 maintained its activity even though exposed to 60° C. for up to 2 hours;
FIG. 8 is the result of desiccation tolerance assay on the bacteriophage ΦCJ11 dried with the aid of a SpeedVac concentrator, in which when titer changes under the dry condition were measured in comparison with pre-drying titers, the activity was maintained at 60° C. for up to 1 hour; and
FIG. 9 is the results of body weight changes due to toxicity after single oral administration of Sprague-Dawley rats with ΦCJ11, in which observation of body weight changes before and 1, 3, 7, 10 and 14 days after administration with ΦCJ11 showed no significant changes in comparison with the control group (▪; male control, □; ΦCJ11 male, ●; female control, ∘; ΦCJ11 female).



BEST MODE
In one aspect to achieve the above objects, the present invention provides a novel bacteriophage having a specific bactericidal activity against Salmonella choleraesuis, Salmonella typhimurium, Salmonella derby, Salmonella infantis or Salmonella newport. 
The present inventors collected fecal and sewage samples from swinery, and isolated therefrom bacteriophages that can lyse the host cell SC. They were also found that these bacteriophages can lyse ST, SD, SI and SN (FIG. 2 and Table 1). A morphological examination under an electron microscope confirmed that the bacteriophage (ΦCJ11) belongs to the family Siphoviridae of morphotype B1 (FIG. 1). Further, the bacteriophage ΦCJ11 was found to have major structural proteins of approximately 69.5 kDa, 55 kDa and 33 kDa, as measured by a protein pattern analysis (FIG. 3), and a genome analysis showed that ΦCJ11 has a total genome size of approximately 97-145.5 kbp (FIG. 4). Furthermore, the results of analyzing its genetic features showed that the bacteriophage includes nucleic acid molecules represented by SEQ ID NOs. 1 to 4 within the total genome (Example 6). Based on SEQ ID NOs. 1 to 4, genetic similarity with other species was compared. It was found that the bacteriophage showed very low genetic similarity with the known bacteriophages, indicating that the bacteriophage is a novel bacteriophage (Table 2). For more detail analysis of genetic features, the ΦCJ11-specific primer sets, namely, SEQ ID NOs. 5 and 6, SEQ ID NOs. 7 and 8, SEQ ID NOs. 9 and 10, and SEQ ID NOs. 11 and 12 were used to perform PCR. Each PCR product was found to have a size of 1.4 kbp, 1.2 kbp, 1.25 kbp and 1.5 kbp (FIG. 5).
Meanwhile, when SC, ST, SD, SI and SN were infected with ΦCJ11, the phage plaques (clear zone on soft agar created by host cell lysis of one bacteriophage) were observed (FIG. 2). The stability of ΦCJ11 was examined under various temperature and pH conditions, resulting in that ΦCJ11 stably maintains in a wide range of pH environments from pH 3.5 to pH 11.0 (FIG. 6) and in high temperature environments from 37° C. to 70° C. (FIG. 7), and even after desiccation (FIG. 8). Also, the wild-type strains SC, ST, SD, SI and SN were also found to fall within the host cell range of ΦCJ11 (Table 3).
Finally, the results of dermal and ocular irritation tests on ΦCJ11 in specific-pathogen-free (SPF) New Zealand White rabbits showed that the primary irritation index (PII) was 0.33, indicating no irritant, and the index of acute ocular irritation (IAOI) was 0 in washing and non-washing groups during the whole experimental periods, indicating no irritant. The results of oral administration of ΦCJ11 showed no changes in weight gain (FIG. 9). As well, mortality, general symptoms (Table 4) and organ abnormality (Table 5) were not observed, indicating no toxicity.
Accordingly, the present inventors designated the bacteriophage as “Bacteriophage ΦCJ11”, in which the bacteriophage was isolated from fecal and sewage samples from swinery and has a specific bactericidal activity against SC, ST, SD, SI and SN and the above characteristics, and deposited at the Korean Culture Center of Microorganisms (361-221, Honje 1, Seodaemun, Seoul) on Sep. 9, 2011 under accession number KCCM11208P.
In another aspect to achieve the above objects, the present invention provides a composition for the prevention or treatment of infectious disease caused by one or more Salmonella bacteria selected from the group consisting of Salmonella choleraesuis, Salmonella typhimurium, Salmonella derby, Salmonella infantis and Salmonella newport, comprising the bacteriophage as an active ingredient.
Having specific bactericidal activity against Salmonella choleraesuis, Salmonella typhimurium, Salmonella derby, Salmonella infantis and Salmonella newport, the bacteriophage of the present invention may be used for the purpose of preventing or treating the diseases caused by these bacteria. Preferably, examples of the infectious diseases include porcine salmonellosis and Salmonella food poisoning caused by Salmonella choleraesuis or Salmonella typhimurium, and acute or chronic porcine enteritis caused by Salmonella derby, Salmonella infantis, Salmonella newport, but are not limited thereto.
As used herein, the term “salmonellosis” refers to symptoms following salmonella infection, such as fever, headache, diarrhea, and vomiting. That is, salmonellosis is an infection with bacteria of the genus Salmonella, which is defined with two clinical forms: an acute septicemic form that resembles typhoid fever and an acute gastroenteritis, including enteritis, food poisoning, and acute septicemia.
As used herein, the term “prevention” means all of the actions in which disease progress is restrained or retarded by the administration of the composition.
As used herein, the term “treatment” means all of the actions in which the condition has taken a turn for the better or been restrained or modified favorably by the administration of the composition.
The composition of the present invention includes ΦCJ11 in an amount of 1×102 to 1×1012 PFU/mL, and preferably in an amount of 1×106 to 1×1010 PFU/mL.
On the other hand, the composition of the present invention may further include a pharmaceutically acceptable carrier.
As used herein, the term “pharmaceutically acceptable carrier” refers to a carrier or diluent that does not cause significant irritation to an organism and does not abrogate the biological activity and properties of the administered compound. For formulation of the composition into a liquid preparation, a pharmaceutically acceptable carrier which is sterile and biocompatible may be used such as saline, sterile water, Ringer's solution, buffered physiological saline, albumin infusion solution, dextrose solution, maltodextrin solution, glycerol, ethanol, and mixtures of one or more thereof. If necessary, other conventional additives such as antioxidants, buffers, and bacteriostatic agents may be added. Further, diluents, dispersants, surfactants, binders and lubricants may be additionally added to the composition to prepare injectable formulations such as aqueous solutions, suspensions, and emulsions, or oral formulations such as pills, capsules, granules, or tablets.
The prophylactic or therapeutic compositions of the present invention may be applied or sprayed to the afflicted area, or administered by oral or parenteral routes. The parenteral administration may include intravenous, intraperitoneal, intramuscular, subcutaneous or topical administration.
The dosage suitable for applying, spraying, or administrating the composition of the present invention will depend upon a variety of factors including formulation method, the mode of administration, the age, weight, sex, condition, and diet of the patient or animal being treated, the time of administration, the route of administration, the rate of excretion, and reaction sensitivity. A physician or veterinarian having ordinary skill in the art can readily determine and prescribe the effective amount of the composition required.
Examples of the oral dosage forms including the composition of the present invention as an active ingredient include tablets, troches, lozenges, aqueous or emulsive suspensions, powder or granules, emulsions, hard or soft capsules, syrups, or elixirs. For formulation such as tablets and capsules, the following are useful: a binder such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, cellulose or gelatin; an excipient such as dicalcium phosphate; a disintegrant such as corn starch or sweet potato starch; and a lubricant such as magnesium stearate, calcium stearate, sodium stearylfumarate, or polyethylene glycol wax. For capsules, a liquid carrier such as lipid may be further used in addition to the above-mentioned compounds.
The parenteral dosage forms including the composition of the present invention as an active ingredient may be formulated into injections via subcutaneous, intravenous, or intramuscular routes, suppositories, or sprays inhalable via the respiratory tract, such as aerosols. Injection forms may be prepared by dissolving or suspending the composition of the present invention, together with a stabilizer or a buffer, in water and loading the solution or suspension onto ampules or vial unit forms. For sprays, such as aerosols, a propellant for spraying a water-dispersed concentrate or wetting powder may be used in combination with an additive.
In still another aspect to achieve the above objects, the present invention provides an antibiotic comprising the bacteriophage as an active ingredient.
As used herein, the term “antibiotic” means any drug that is applied to animals to kill pathogens, and used herein as a general term for antiseptics, bactericidal agents and antibacterial agents. The animals are mammals including human. The bacteriophage of the present invention, unlike the conventional antibiotics, has a high specificity to Salmonella so as to kill the specific pathogens without affecting beneficial bacteria, and does not induce drug resistance so that it can be provided as a novel antibiotic with a comparatively long life cycle.
In still another aspect to achieve the above objects, the present invention provides an animal feed or drinking water comprising the bacteriophage as an active ingredient.
In-feed antibiotics used in the livestock and fishery industries are intended to prevent infections. However, most of the currently available in-feed antibiotics are problematic in that they are apt to induce the occurrence of resistant strains and may be transferred to humans, due to remaining in livestock products. The uptake of such residual antibiotics may make human pathogens resistant to antibiotics, resulting in the spread of diseases. In addition, since there are a variety of in-feed antibiotics, the increasing global emergence of multidrug-resistant strain is a serious concern. Therefore, the bacteriophage of the present invention can be used as an in-feed antibiotic that is more eco-friendly and able to solve the above problems.
The animal feed of the present invention may be prepared by adding the bacteriophage directly or in separate feed additive form to an animal feed. The bacteriophage of the present invention may be contained in the animal feed as a liquid or in a solid form, preferably in a dried powder. The drying process may be performed by air drying, natural drying, spray drying, and freeze-drying, but is not limited thereto. The bacteriophage of the present invention may be added as a powder form in an amount of 0.05 to 10% by weight, preferably 0.1 to 2% by weight, based on the weight of animal feed. The animal feed may also include other conventional additives for long-term preservation, in addition to the bacteriophage of the present invention.
The feed additive of the present invention may additionally include other non-pathogenic microorganisms. The available additional microorganism may be selected from the group consisting of Bacillus subtilis that can produce protease, lipase and invertase, Lactobacillus sp. strain that can exert physiological activity and a function of decomposing under anaerobic conditions, such as in the stomach of cattle, filamentous fungi including Aspergillus oryzae (J Animal Sci 43:910-926, 1976) that increases the weight of domestic animals, enhances milk production and helps the digestion and absorptiveness of feeds, and yeast including Saccharomyce scerevisiae (J Anim Sci 56:735-739, 1983).
The feed including ΦCJ11 of the present invention may include plant-based feeds, such as grain, nut, food byproduct, seaweed, fiber, drug byproduct, oil, starch, meal, and grain byproduct, and animal-based feeds such as protein, inorganic matter, fat, mineral, fat, single cell protein, zooplankton, and food waste, but is not limited thereto.
The feed additive including ΦCJ11 of the present invention may include binders, emulsifiers, and preservatives for the prevention of quality deterioration, amino acids, vitamins, enzymes, probiotics, flavorings, non-protein nitrogen, silicates, buffering agents, coloring agents, extracts, and oligosaccharides for efficiency improvement, and other feed premixtures, but is not limited thereto.
Further, the supply of drinking water mixed with the bacteriophage of the present invention can reduce the number of Salmonella bacteria in the intestine of livestock, thereby obtaining Salmonella-free livestock.
In still another aspect to achieve the above objects, the present invention provides a sanitizer or cleaner comprising the bacteriophage as an active ingredient.
In still another aspect to achieve the above objects, the present invention provides a method for treating infectious diseases caused by Salmonella choleraesuis, Salmonella typhimurium, Salmonella derby, Salmonella infantis or Salmonella newport using the bacteriophage or the composition.
In detail, the therapeutic method of the present invention comprises the step of administering a pharmaceutically effective amount of the bacteriophage or the composition to an individual having infectious diseases caused by Salmonella choleraesuis, Salmonella typhimurium, Salmonella derby, Salmonella infantis or Salmonella newport. 
The bacteriophage or the composition of the present invention may be administered in the form of a pharmaceutical formulation into animals or may be ingested as a mixture with animal feed or drinking water by animals and preferably as a mixture with animal feed.
As long as it reaches target tissues, any route, whether oral or parenteral, may be taken for administering the bacteriophage or the composition of the present invention. In detail, the composition of the present invention may be administered in a typical manner via any route such as oral, rectal, topical, intravenous, intraperitoneal, intramuscular, intraarterial, transdermal, intranasal, and inhalation routes.
It will be obvious to those skilled in the art that the total daily dose of the bacteriophage or the composition of the present invention to be administered by the therapeutic method should be determined through appropriate medical judgment by a physician. Preferably, the therapeutically effective amount for given patients may vary depending on various factors well known in the medical art, including the kind and degree of the response to be achieved, the patient's condition such as age, body weight, state of health, sex, and diet, time and route of administration, the secretion rate of the composition, the time period of therapy, concrete compositions according to whether other agents are used therewith or not, etc.
Mode for Invention
Hereinafter, the present invention will be described in more detail with reference to Examples. However, these Examples are for illustrative purposes only, and the invention is not intended to be limited by these Examples.
Example 1
Salmonella Bacteriophage Isolation
Example 1-1
Bacteriophage Screening and Single Bacteriophage Isolation
50 mL of sample from swinery and sewage effluent was transferred to a centrifuge tube, and centrifuged at 4000 rpm for 10 minutes. Then, the supernatant was filtered using a 0.45 μm filter. 18 mL of sample filtrate was mixed with 150 μl of Salmonella choleraesuis (“SC”) shaking culture medium (OD600=2) and 2 mL of 10× Luria-Bertani medium (tryptone 10 g/L, yeast extract 5 g/L and NaCl 10 g/L: LB medium). The mixture was cultured at 37° C. for 18 hours, and the culture medium was centrifuged at 4000 rpm for 10 minutes. The supernatant was filtered using a 0.2 μm filter. 3 mL of 0.7% agar (w/v) and 150 μl of SC shaking culture medium (OD600=2) were mixed, and plated onto LB plate (“top-agar”), and allowed to solidify. 10 μl of the culture filtrate was spread thereon, and cultured for 18 hours at 37° C., and the titration of phage lysate was performed on the top-agar, called soft agar overlay method.
The sample culture medium containing the phage lysate was properly diluted, and mixed with 150 μl of SC shaking culture medium (OD600=2), followed by soft agar overlay method, so that single plaques were obtained. A single plaque represents one bacteriophage and thus, for isolation of single bacteriophages, one phage plaque was added to 400 μl of SM solution (NaCl, 5.8 g/L, MgSO47H2O, 2 g/L, 1 M Tris-Cl (pH 7.5) 50 ml/L), and left for 4 hours at room temperature to isolate single bacteriophages. To purify the bacteriophage in large quantities, 100 μl of supernatant was taken from the single bacteriophage solution, and mixed with 12 mL of 0.7% agar and 500 μl of SC shaking culture medium, followed by soft agar overlay method on LB plate having a diameter of 150 mm. When lysis was completed, 15 mL of SM solution was added to the plate. The plate was gently shaken for 4 hours at room temperature to elute the bacteriophages from the top-agar. The SM solution containing the eluted bacteriophages was recovered, chloroform was added to a final volume of 1%, and mixed well for 10 minutes. The solution was centrifuged at 4000 rpm for 10 minutes. The obtained supernatant was filtered using a 0.45 μm filter, and stored in the refrigerator.
Example 1-2
Large-Scale Batches of Bacteriophage
The selected bacteriophages were cultured in large quantities using SC. SC was shaking-cultured, and an aliquot of 1.5×1010 cfu (colony forming units) was centrifuged at 4000 rpm for 10 minutes, and the pellet was resuspended in 4 ml of SM solution. The bacteriophage of 9.0×108 PFU (plaque forming unit) was inoculated thereto (MOI: multiplicity of infection=0.001), and left at 37° C. for 20 minutes. The solution was inoculated into 150 ml of LB media, and cultured at 37° C. for 5 hours. Chloroform was added to a final volume of 1%, and the culture solution was shaken for 20 minutes. DNase I and RNase A were added to a final concentration of 1 μg/ml, respectively. The solution was left at 37° C. for 30 minutes. NaCl and PEG (polyethylene glycol) were added to a final concentration of 1 M and 10% (w/v), respectively and left at 4° C. for an additional 3 hours. The solution was centrifuged at 4° C. and 12,000 rpm for 20 minutes to discard the supernatant. The pellet was resuspended in 5 mL of SM solution, and left at room temperature for 20 minutes. 4 mL of chloroform was added thereto and mixed well, followed by centrifugation at 4° C. and 4000 rpm for 20 minutes. The supernatant was filtered using a 0.2 μm filter, and the bacteriophage was purified by glycerol density gradient ultracentrifugation (density: 40%, 5% glycerol at 35,000 rpm and 4° C. for 1 hour). The purified bacteriophage was designated as “Bacteriophage ΦCJ11”, and resuspended in 300 μl of SM solution, followed by titration. The bacteriophage φCJ11 was deposited at the Korean Culture Center of Microorganisms (361-221, Honje 1, Seodaemun, Seoul) on Sep. 9, 2011 under accession number KCCM11208P.
Example 2
Examination on φCJ11 Infection of Salmonella 
To analyze the selected bacteriophage for lytic activity on Salmonella species other than SC, attempts were made of cross infection with other Salmonella species. As a result, ΦCJ11 infected SC (Salmonella choleraesuis), ST (Salmonella typhimurium), SD (Salmonella derby), SN (Salmonella newport), SI (Salmonella infantis), SA (Salmonella arizonae) and SB (Salmonella bongori), but did not infect SE (Salmonella enteritidis), SG (Salmonella gallinarum), and SP (Salmonella pullorum) (Table 1 and FIG. 2).







TABLE 1







ΦCJ11 Infection of Salmonella














Phage


Phage


Sero-

plaque
Sero-

plaque


type
Strain name
formation
type
Strain name
formation





SC
ATCC 10708
◯
SA
ATCC 12398
◯


SN
SL 317
◯
SB
ATCC 12397
◯


SD
ATCC 2468
◯
ST
13
◯


SE
SGSC 2282
X
SI
SARB 26
◯


SG
SGSC 2293
X
SP
SGSC 2295
X





* ATCC: American Type Culture Collection


* SGSC: Salmonella Genetic Stock Center






Moreover, FIG. 2 is a photograph showing the formation of ΦCJ11 plaques in a lawn of salmonella bacteria. As shown in FIG. 2 (A,B;SC, C;SG, D;ST, E;SI, F,G;SD, H;SN), plaque formation was observed in lawns of SC, ST, SI, SD and SN, but not in lawns of SG.
Example 3
Morphology of #CJ11
The purified ΦCJ11 was diluted in the SM buffer solution, and then mounted on a copper grid, stained with 2% uranyl acetate for 3 to 5 seconds, and dried. Examination under a transmission electron microscope (LIBRA 120, Carl Zeiss transmission electron Microscope, 80 kV, magnification of ×120,000 ̃×200,000) was performed (FIG. 1). FIG. 1 is an electron microscopy photograph of ΦCJ11. As shown in FIG. 1, it was found that the purified ΦCJ11 belongs to the family Siphoviridae of morphotype B1, characterized by an isometric capsid and a long non-contractile tail.
Example 4
Protein Pattern Analysis of ΦCJ11
15 μL of a ΦCJ11 solution purified at a titer of 1011 PFU/mL was mixed with 3 μL of a 5×SDS sample solution, and heated for 5 minutes. 12% SDS-PAGE was performed, and then the gel was stained with Coomassie blue for 1 hour at room temperature (FIG. 3). FIG. 3 is the result of SDS-PAGE of the isolated bacteriophage ΦCJ11, in which Precision plus protein standard (BIO-RAD) was used as a marker. As shown in FIG. 3, the major proteins were detected at 33 kDa, 55 kDa and 69.5 kDa.
Example 5
Analysis of Total Genomic DNA Size of ΦCJ11
Genomic DNA of the purified ΦCJ11 was isolated using ultracentrifugation. In detail, to the purified ΦCJ11 culture medium were added EDTA (ethylenediaminetetraacetic acid (pH 8.0)), proteinase K, and SDS (sodium dodecyl sulfate) at a final concentration of 20 mM, 50 μg/mL, and 0.5% (w/v), respectively, followed by incubation at 50° C. for 1 hour. An equal volume of phenol (pH 8.0) was added and mixed well. After centrifugation at room temperature and 12,000 rpm for 10 minutes, the supernatant was mixed well with an equal volume of PC (phenol:chloroform=1:1). Another centrifugation was performed at room temperature and 12,000 rpm for 10 minutes. Then, a supernatant was obtained, and mixed with an equal volume of chloroform, followed by centrifugation at room temperature and 12,000 rpm for 10 minutes. The obtained supernatant was mixed with 1/10 volume of 3 M sodium acetate and two volumes of cold 95% ethanol, and left at −20° C. for 1 hour. After centrifugation at 0° C. and 12,000 rpm for 10 minutes, the supernatant was completely removed, and the DNA pellet was dissolved in 50 μL of TE (Tris-EDTA (pH 8.0)). The extracted DNA was diluted 10-fold, and measured for absorbance at OD260 to determine its concentration. 1 μg of the total genomic DNA was loaded onto 1% PFGE (pulse-field gel electrophoresis) agarose gel, and electrophoresed at 14° C. for 22 hours in a BIORAD CHEF DR II PFGE system under the conditions of switch time ramp for 50-90 seconds, 6 V/cm (200V). The CHEF DNA Size Standard Lambda Ladder (Bio-Rad) was used as a DNA size marker (FIG. 4). FIG. 4 is the result of PFGE of the isolated bacteriophage ΦCJ11. As shown in FIG. 4, DNA of approximately 140 kbp present between 48.5 to 1,000 kbp was observed.
Example 6
Genetic Analysis of ΦCJ11
For the genetic analysis of the purified ΦCJ11, 5 μg of the genomic DNA of ΦCJ11 was double digested with the restriction enzymes PstI, XbaI and BamHI, EcoRI and SalI. The vector pCL1920 (Promega) was digested with the restriction enzymes PstI, XbaI and BamHI, EcoRI and SalI, and then treated with CIP (calf intestinal alkaline phosphatase). The digested genomic DNA was mixed at a ratio of 3:1 with the vector, and ligated at 16° C. for 2 hours. The resulting recombinant vector was transformed into E. coli DH5a which was then plated on an LB plate containing specinomycin and X-gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) for selection of blue/white colonies. The selected colonies were cultured for 16 hours in a culture medium containing the antibiotic with shaking. Then, plasmids were extracted using a Plasmid purification kit (Promega).
The cloning of the plasmids was confirmed by PCR using primer sets of FTR135 and FTR136 (SEQ ID NOs. 13 and 14) and selection was made only of insert fragments having a size of 1 kb or longer. Their nucleotide sequences were analyzed using the primer sets. The nucleotide sequences thus obtained were given in SEQ ID NOs. 1 to 4, respectively, each having a size of 1 to 2 kbp or less, and analyzed for sequence similarity with the aid of NCBI blastx and blastn program, and the results are summarized in Table 2, below.







TABLE 2







Comparison of Sequence Similarity between ΦCJ11 and Other Bacteriophages









Blastx














Organism
Protein
Query
Subject
Identity
e-value

















1
Enterobacteria
hypothetical protein
436-627
1-64 
63/64
7e−32



phage T5



(98%)



Enterobacteria
hypothetical protein
 10-423
1-139
127/139
3e−31



phage SPC35



(91%)



Enterobacteria
hypothetical protein
 3-863
30-315 
264/291
 2e−116



phage SPC35



(91%)



Enterobacteria
hypothetical protein
 3-863
30-315 
255/291
 1e−113



phage T5



(88%)



Enterobacteria
hypothetical protein
 3-761
29-281 
235/253
 7e−107



phage EPS7



(93%)



Klebsiella
hypothetical protein
 9-761
22-276 
217/255
 4e−100



phage KP15



(85%)



Enterobacteria
putative SPFH domain-
 9-839
18-257 
223/281
7e−99



phage RB43
containing protein


(79%)



Enterobacteria
hypothetical protein
 9-761
18-272 
219/255
2e−98



phage RB16



(86%)


2
Enterobacteria
hypothetical protein
412-771
1-120
109/120
7e−57



phage EPS7



(91%)



Enterobacteria
hypothetical protein
490-771
1-94 
94/94
3e−46



phage T5



(100%) 



Enterobacteria
D11 protein
532-2 
1-177
175/177
5e−96



phage T5



(99%)



Enterobacteria
D11 protein
532-2 
1-177
174/177
2e−95



phage SPC35



(98%)



Enterobacteria
D11 protein
532-8 
1-175
163/175
4e−90



phage EPS7



(93%)



Enterobacteria
hypothetical protein
872-528
5-120
 96/116
2e−46



phage EPS7



(83%)


3
Enterobacteria
tail protein Pb4
777-4 
1-258
221/258
 4e−128



phage SPC35



(86%)



Enterobacteria
tail protein Pb4
777-4 
1-258
197/258
 6e−115



phage T5



(76%)



Enterobacteria
tail protein Pb3
1322-780 
769-949 
178/181
2e−96



phage SPC35



(98%)



Enterobacteria
tail protein Pb3
1322-780 
769-949 
160/181
9e−88



phage EPS7



(88%)



Enterobacteria
structural tail
1322-780 
769-949 
156/181
8e−84



phage T5
protein Pb3


(86%)



Enterobacteria
tail protein Pb4
423-4 
1-140
112/140
2e−62



phage EPS7



(80%)


4
Enterobacteria
flap endonuclease
980-564
153-291 
138/139
1e−73



phage SPC35



(99%)



Enterobacteria
flap endonuclease
980-564
153-291 
138/139
1e−73



phage T5



(99%)



Enterobacteria
flap endonuclease
980-564
153-291 
135/139
4e−72



phage EPS7



(97%)



Enterobacteria
putative deoxyUTP
564-157
1-136
130/136
3e−70



phage SPC35
pyrophosphatase


(96%)



Enterobacteria
putative deoxyUTP
564-160
1-135
130/135
2e−69



phage T5
pyrophosphatase


(96%)



Enterobacteria
putative deoxyUTP
564-157
1-136
128/136
2e−67



phage EPS7
pyrophosphatase


(94%)









Example 7
Design of ΦCJ11-Specific Primer Sequences
In order to identify ΦCJ11, ΦCJ11-specific primers were designed on the basis of SEQ ID NOS. 1 to 4. PCR was performed using each primer set of SEQ ID NOS. 5 and 6, SEQ ID NOs. 7 and 8, SEQ ID NOs. 9 and 10, and SEQ ID NOs. 11 and 12. 0.1 μg of the genomic DNA of bacteriophage and 0.5 pmol of each primer were added to a pre-mix (Bioneer), and the final volume was adjusted to 20 μL. PCR was performed with 30 cycles of denaturation; 94° C. 30 seconds, annealing; 55° C. 30 seconds, and polymerization; 72° C., 1.5 minutes (FIG. 5). FIG. 5 is the result of PCR, performed using each primer set for the ΦCJ11 genomic DNA. A; a primer set of SEQ ID NOs. 5 and 6, B; a primer set of SEQ ID NOs. 7 and 8, C; a primer set of SEQ ID NOs. 9 and 10, and D; a primer set of SEQ ID NOs. 11 and 12. All of A, B, C and D lanes had PCR products of approximately 1 to 2 kbp. As shown in FIG. 5, the PCR products thus obtained had a size of approximately 1 kbp or more to 2 kbp or less, with the primer sets of SEQ ID NOs. 5 and 6, SEQ ID NOs. 7 and 8, SEQ ID NOs. 9 and 10, and SEQ ID NOs. 11 and 12.
Example 8
pH Stability of Bacteriophage
In order to determine whether ΦCJ11 survives with stability under the low pH environment in the stomach of pig, ΦCJ11 was assayed for stability in a wide range of pH (pH 2.1, 2.5, 3.0, 3.5, 4.0, 5.5, 6.4, 6.9, 7.4, 8.2, 9.0, 9.8 and 11.0). Various pH solutions (sodium acetate buffer (pH 2.1, pH 4.0, pH 5.5, and pH 6.4), sodium citrate buffer (pH 2.5, pH 3.0, and pH 3.5), sodium phosphate buffer (pH 6.9 and pH 7.4) and Tris-HCl (pH 8.2, pH 9.0, pH 9.8 and pH 11.0)) were prepared to have a concentration of 0.2 M. 180 μL of each pH solution was mixed with 20 μL of a bacteriophage solution (1.0×1011 PFU/mL) to give each pH solution a concentration of 1 M, followed by incubation at room temperature for 2 hours. The reaction solution was serially diluted, and 10 μL of each dilution was cultured at 37° C. for 18 hours by a soft agar overlay method to determine the titers of the phage lysates (FIG. 6). FIG. 6 is the result of acid-resistance assay on the bacteriophage ΦCJ11, showing the number of surviving bacteriophage at pH 2.1, 2.5, 3.0, 3.5, 4.0, 5.5, 6.4, 6.9, 7.4, 8.0, 9.0, 9.8 and 11.0. The bacteriophage ΦCJ11 did not lose its activity until pH 5.5. However, the bacteriophage ΦCJ11 showed reduced activity at pH 4 and pH 3.5, and completely lost its activity at pH 3.0 or lower, as compared to a control. As shown in FIG. 6, the bacteriophage did not lose its activity and remained stable down to pH 5.5 whereas it lost its activity at pH 3.0 or lower.
Example 9
Heat Stability of Bacteriophage
For use as a feed additive, the bacteriophage was assayed for stability to the heat generated during a formulation process. In this regard, 200 μL of a ΦCJ11 solution with a titer of 1.0×1011 PFU/mL was incubated at 37° C., 45° C., 53° C., 60° C., and 70° C. for 0 minute, 10 minutes, 30 minutes, 60 minutes and 120 minutes. The solution was serially diluted, and 10 μL of each dilution was cultured at 37° C. for 18 hours by a soft agar overlay method to determine the titers of phage lysates (FIG. 7). FIG. 7 is the result of heat-resistance assay on the bacteriophage ΦCJ11, showing the number of surviving bacteriophage at 37° C., 45° C., 53° C., 60° C., and 70° C. for 0, 10, 30, 60 and 120 minutes. As shown in FIG. 7, the bacteriophage ΦCJ11 maintained its activity even though exposed at 60° C. up to 2 hours.
Example 10
Desiccation Tolerance of Bacteriophage
For use as a feed additive, the bacteriophage ΦCJ11 was assayed for tolerance to the dry condition set for a formulation process. On the basis of the results obtained from the heat stability assay, a desiccation assay was performed using a SpeedVac concentrator. 200 μL of a ΦCJ11 solution having a titer of 1.0×1011 PFU/mL was dried under vacuum at 60° C. for 2 hours, and the pellet thus obtained was completely re-suspended in 200 μL of the SM solution at 4° C. for one day, and measured for titer values (FIG. 8). FIG. 8 is the result of desiccation tolerance assay on the bacteriophage ΦCJ11 dried with the aid of a SpeedVac concentrator. As shown in FIG. 8, when titer changes under the dry condition were measured in comparison with pre-drying titers, the activity was maintained at 60° C. up to 1 hour.
Example 11
Infection Spectrum of Bacteriophage
ΦCJ11 was assayed for lytic activity against the wild-type (2 strains), Salmonella choleraesuis (5 strains), Salmonella typhimurium (17 strains), Salmonella infantis (4 strains), Salmonella newport (6 strains), Salmonella derby (2 strains) and Salmonella dublin (3 strains), obtained from Laboratory of Avian Diseases, College of Veterinary Medicine, Seoul National University, in addition to SC (ATCC SC10708) used in the experiment. 150 μL of each strain shaking culture medium (OD600=2) was mixed, and 10 μL of ΦCJ11 solution having a titer of 1010 PFU/mL was cultured at 37° C. for 18 hours using a soft agar overlay method to monitor the was observed in 8 strains of SC.







TABLE 3







Wild-type strains SC, ST, SD, SI, SN infected by ΦCJ11














Phage


Phage




plaque


plaque


Serotype
Strain name
formation
serotype
Strain name
formation

















SC
S. choleraesuis
ATCC
∘
SI
S. infantis
SARB 26
∘




2929



S. choleraesuis
ATCC
∘

S. infantis
SARB 27
∘




2930



S. choleraesuis
ATCC
∘

S. infantis
S1326/28
∘




2932



S. choleraesuis
ATCC
∘

S. infantis
B09-106
∘




2933



S. choleraesuis
ATCC
∘
SN
S. newport
SARB 36
∘




2425



S. choleraesuis
ATCC
∘

S. newport
SARB 37
∘




10708



S. choleraesuis
SNU#1
∘

S. newport
SARB 38
∘



S. choleraesuis
SNU#2
∘

S. newport
7257
∘


ST
S. typhimurium
SNU
∘

S. newport
SL 317
∘




ST1



S. typhimurium
SNU
∘

S. newport
SL 254
∘




ST2



S. typhimurium
SNU
∘
SD
S. derby
ATCC
∘




ST4



2466



S. typhimurium
SNU
∘

S. derby
ATCC
∘




ST7



2468



S. typhimurium
SNU
∘
SD
S. dublin
SA 4405
∘




ST8



S. typhimurium
SNU
∘

S. dublin
RKS 4699
∘




ST11



S. typhimurium
SNU
∘

S. dublin
88/6
∘




ST12



S. typhimurium
SNU
∘
SA
S. arizonae
ATCC
∘




ST13



12398



S. typhimurium
SNU
∘
SB
S. bongori
ATCC
∘




ST14



12397



S. typhimurium
SNU
∘
SH
S. heidelberg
SARA 33
∘




ST17



S. typhimurium
SNU
∘

S. heidelberg
SARA 23
∘




ST18



S. typhimurium
SNU
∘
SM
S. maimi
SARB 28
∘




ST19



S. typhimurium
SNU
∘

S. maimi
SARB 29
∘




ST20



S. typhimurium
SNU
∘
SP
S. panama
SARB 39
∘




ST26



S. typhimurium
SNU
∘

S. panama
SARB 40
∘




ST38



S. typhimurium
SNU
∘

S. panama
SARB 41
∘




ST41



S. typhimurium
SNU
∘

S. panama
7261
∘




ST42





* SGSC: Salmonella genetic stock center


* ATCC: American Type Culture Collection


* SNU: Laboratory of Avian Diseases, College of Veterinary Medicine, Seoul National University






Example 12
Toxicity Assay of Bacteriophage
Dermal and ocular irritation tests were performed in specific-pathogen-free (SPF) New Zealand white rabbits, which are commonly used in the toxicity test of bacteriophage ΦCJ11 for the prevention of salmonellosis and salmonella food poisoning, and of which experimental data were accumulated to allow easy analysis of experiment results. The normal abdominal skin (non-injured skin) and injured abdominal skin of rabbits were covered and contacted with 2.5 cm×2.5 cm of gauze applied with the test substance, and each 0.5 mL/site was applied. No changes in general symptoms were observed, and a slight weight loss was observed 1 day after application of the test substance, which can likely to be attributed to stress due to occlusive application of the test substance. In the dermal irritation test, the primary irritation index (PII) was 0.33, indicating no irritant. For the ocular irritation test, the left eye of a rabbit was applied with the test substance, and then compared to the right eye, which was not applied with the test substance. During the experimental period, general symptoms and abnormal changes in body weight related to application of the test substance were not observed. After application of the test substance, the eye examination showed that the index of acute ocular irritation (IAOI) was “0”, indicating no irritant. Therefore, these results indicate that the novel bacteriophage ΦCJ11 has no toxicity.
Further, toxicity assay was performed by single oral administration of Sprague-Dawley rats with ΦCJ11. A test substance-administered group treated with 1×1011 PFU/kg of ΦCJ11 and an excipient control group treated with a vehicle [20 mM Tris-HCl (pH 7.0)+2 mM MgCl] as an excipient were prepared, and 10 rats of each group (5 each of female and male sexes) were orally administered with a single dosage. Mortality, general symptoms, changes in body weight, and autopsy findings were monitored for 2 weeks and compared to each other. Monitoring was conducted every 6 hours, starting from 30 minutes to 1 hour after administration on the day of administration. Then, general symptoms were monitored once a day for 14 days, and recorded thereof (Tables 4 and 5).







TABLE 4







Mortality and general symptoms after oral administration of ΦCJ11











Done
Day after treatment
























Sex
(pfu)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
Mortality





Male
Control
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0



1011
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0


Female
Control
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0



1011
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
















TABLE 5







Autopsy findings after oral administration of ΦCJ11












Sex
Done (PFU)
gross finding
FrequencyA







Male
Control
No gross finding
5/5




1011
No gross finding
5/5



Female
Control
No gross finding
5/5




1011
No gross finding
5/5







ANumber of animals with the sign/number of animals examined.






As shown in Tables 4 and 5, none of them died, and neither toxic symptoms nor noticeable clinical symptoms were generated by ΦCJ11. The results are summarized in Tables 4 and 5. Body weights were recorded before administration and 1, 3, 7, 10 and 14 days after administration. No significant changes were observed in body weight compared to the control group.
Meanwhile, the results of body weight changes indicate that ΦCJ11 does not cause a toxic reaction sufficient to reduce appetite or to change body weight. These results are shown in FIG. 9. FIG. 9 is the results of body weight changes due to toxicity after single oral administration of Sprague-Dawley rats with ΦCJ11. As shown in FIG. 9, observation of body weight changes before administration and 1, 3, 7, 10 and 14 days after administration with ΦCJ11 showed that no significant changes in body weight were found in comparison with the control group (▪; male control, □; ΦCJ11 male, ●; female control, ◯; ΦCJ11 female).
Therefore, it was found that ADL of the novel bacteriophage ΦCJ11 exceeds 1×1011 PFU/kg in both female and male rats, and thus it is non-toxic.

## Claims

1. A method for restraining or retarding progress of, or treating, infectious diseases caused by one or more Salmonella bacteria selected from the group consisting of Salmonella choleraesuis, Salmonella typhimurium, Salmonella derby, Salmonella infantis, Salmonella Newport, and combinations thereof, comprising administering a bacteriophage identified by accession number KCCM11208P to animals in need thereof.

2. A method for restraining or retarding progress of, or treating, infectious diseases caused by one or more Salmonella bacteria selected from the group consisting of Salmonella choleraesuis, Salmonella typhimurium, Salmonella derby, Salmonella infantis, Salmonella Newport, and combinations thereof, comprising administering a composition to animals in need thereof,
wherein the composition comprises a bacteriophage as an active ingredient and a pharmaceutically acceptable carrier,
wherein the bacteriophage is identified by accession number KCCM11208P.