# S507150



# Test Report

Measurement of the molecular weights

”FMN-809(Original) and Stability tested samples”

TORAY RESEARCH CENTER, INC.

3-3-7 Sonoyama

㧘 Otsu City

㧘 Shiga 520-8567

㧘

JAPAN 㼀㻿㻙㻴㻞㻜㻝㻼㻼 㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝

㻼㼍㼓㼑㻌㻝㻌㻛㻞㻢



# CBI

CBI

# S507150

-1- Toray Research Center, Inc.

ޣ Summary ޤ

The GPC measurements of the original sample and the stability tested samples were

performed. The results are shown in Table A. The content of components having molecular

weight of less than 1000 was calculated of the original sample. The results are shown in Table B.

The average molecular weights and the molecular weight distribution curves of the stability

tested samples were almost same as those of the original sample.

The content of the component having molecular weight of less than MW 1000 was 0.92% of

the original sample.

Table A Average molecular weights *1



# Sample M

n *2 

# M

w *2 

# M

z *2 

# M

w / M

n M

z / M

w

pH 1.2 12800 39100 65600 3.05 1.68

pH 4.0 13200 39400 65800 2.98 1.67

pH 7.0 13300 39300 66100 2.95 1.68

pH 9.0 12800 39200 66100 3.06 1.69

Original 14000 39800 66900 2.84 1.68

*1 Relative values of polystyrene standards and average of the twice measurements

*2 M

n : Number average molecular weight

*2 M

w : Weight average molecular weight

*2 M

z : Z average molecular weight



# Table B Contents of components less than

MW 1000 of the original sample*

Run 1 0.98%

Run 2 0.85%

Average 0.92%

* Calculated values from polystyrene standards

molecular weight 㼀㻿㻙㻴㻞㻜㻝㻼㻼 㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝

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# CBI

CBI

CBI

CBI

# S507150

-2- Toray Research Center, Inc. 1. Sponsor

Name Daikin Industries, LTD.

Address 1-1, Nishi-Hitotsuya, Settsu, Osaka 566-8585, Japan

2. Test Laboratory

Name Toray Reserch Center, Inc.

Address 3-3-7 Sonoyama, Otsu City, Shiga, 520-8567, Japan

3. Objective

The objective of this measurement are to confirm of having molecular weight change or not

before and after stability test and to obtain contents of components having molecular weight of

less than 1000 of FMN-809.

4. Sample

copolymer of 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctyl 2-chloro-2-propenate / stearyl

acrylate (Abbreviated title: FMN-809)

5. Experimental

5.1 Principle

Gel permeation chromatography (GPC) is a family of the liquid chromatography that

separates molecules of the polymer sample by the difference in their molecular size. The polymer

molecules are separated in the column packed with the gels that have the pore of the same size as

the polymer size in dilute solution (hydrodynamic volume). The high molecular weight

component is eluted faster than the low molecular weight component because the high molecular

weight component is slightly permeable into the pore of the gel. Therefore GPC provides the

molecular weight distribution curve and the average molecular weights of the polymer.

5.2 Measurement Parameters

Apparatus Gel Permeation Chromatograph (GPC-MALS1)



# Detector Differential refractive index detector RI-8020 (TOSOH)

Data Processing system GPC Data Processing System (Toray research Center)



# Column set Column set of two TSKgel GMH XL 

# and one TSKgel G2500H XL



# (TOSOH)

Mobile Phase Tetrahydrofuran 㼀㻿㻙㻴㻞㻜㻝㻼㻼 㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝

㻼㼍㼓㼑㻌㻟㻌㻛㻞㻢



# CBI

CBI

CBI

CBI

CBI

# S507150

-3- Toray Research Center, Inc. Flow rate 1.0 mL/min



# Temperature 23 r 2 ͠



# Sample Concentration 0.20% (w/v)



# Solubility Soluble



# Filtration 0.45 P m-Millex-LH (Millipore)



# Injection volume 0.200 mL



# the calibration curve of

the standards Polystyrene (PS) standards (TOSOH)



# 6. Results

6.1 GPC Curve

GPC curves of the sample are shown in Fig.1 to Fig.10. The signal of the sample was

detected from about 20 min. to 31 min. The peaks detected after about 31 min. of the elution time

were the solvent-composition-change, solvent-impurity and/or the system peak (It was confirmed

by the GPC measurement of the solvent. See Fig.11).

6.2 Calibration curve of molecular weight

The calibration curve of p olystyrene (PS) 

# standards is 

# shown in Fig.12. The calibration curve

was obtained from the relationship between the molecular weight and elution time under the

same GPC conditions. In this report the calculated molecular weights were relative values of PS

standards.

3

3 2

2 1 0 

# log t A t A t A A M

 

 

  (1)

where, M : Molecular weight

t : Elution time

A i : Coefficients

6.3 Calculation of molecular weight distr ibution (MWD) and average molecular weights



# The MWD was originated by the GPC curves of the sample (Fig.1 to Fig.10) and the

calibration curve (Fig.12) using GPC data processing system. The MWD curves and the average

molecular weights are shown in Fig.13 to Fig.22 and Table 1.

The average molecular weights were calculated according to Eqs. (2), (3) and (4). 㼀㻿㻙㻴㻞㻜㻝㻼㻼 㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝

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# CBI

# S507150

-4- Toray Research Center, Inc. M

n = Ǜ ( N

i ࡮ M i )/ Ǜ N i (2)

M

w = Ǜ ( N

i ࡮ M i 2 )/ Ǜ ( N

i ࡮ M i ) (3)

M

z = Ǜ ( N

i ࡮ M i 3 )/ Ǜ ( N

i ࡮ M i 2 ) (4)

where, M

n : Number average molecular weight

M

w : Weight average molecular weight

M

z : Z average molecular weight

N

i : Molar fraction at elution time t i

M

i : Molecular weight at elution time t i

The polydispersity ( M

w / M

n , M

z / M

w ), indicating the index of the dispersion of MWD, is

shown in Table A.

where , M

w / M

n

: Polydispersity

(the index of the dispersion in the low molecular weight region)

M

z / M

w : Polydispersity

(the index of the dispersion in the high molecular weight region)

6.4 Content of the components less than molecular weight 1000 of the original sample

The MWD data are shown in Table 2 and Table 3. The content of the components having the

molecular weight of less than MW 1000 was 0.92% (average of 0.98% and 0.85%).

7. Conclusion

The GPC measurements of the original sample and the stability tested samples were

performed. The results are shown in Table A. The content of components having molecular

weight of less than 1000 was calculated of the original sample. The results are shown in Table B.

The average molecular weights and the molecular weight distribution curves of the stability

tested samples were almost same as those of the original sample.

The content of the component having molecular weight of less than MW 1000 was 0.92% of

the original sample. 㼀㻿㻙㻴㻞㻜㻝㻼㻼 㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝

㻼㼍㼓㼑㻌㻡㻌㻛㻞㻢



# CBI

CBI

CBI

# 㼀㻿㻙㻴㻞㻜㻝㻼㻼 㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝

㻼㼍㼓㼑㻌㻢㻌㻛㻞㻢



# CBI㪪㪌㪇㪎㪈㪌㪇1 12900 39300 66000 3.05 1.68 Fig.152 12700 38900 65100 3.06 1.67 Fig.16Average 12800 39100 65600 3.05 1.681 13100 39300 65700 3.00 1.67 Fig.172 13300 39400 65800 2.96 1.67 Fig.18Average 13200 39400 65800 2.98 1.671 13400 39300 66100 2.93 1.68 Fig.192 13100 39300 66000 3.00 1.68 Fig.20Average 13300 39300 66100 2.95 1.681 12500 39100 65900 3.13 1.69 Fig.212 13000 39300 66200 3.02 1.68 Fig.22Average 12800 39200 66100 3.06 1.691 13800 39700 66500 2.88 1.68 Fig.132 14200 39800 67200 2.80 1.69 Fig.14Average 14000 39800 66900 2.84 1.68* Relative values of polystyrene standardspH 7.0Test solutionMnMwpH 1.2pH 4.0OriginalMolecular WeightDistributionTable 1Average Molecular Weight*RunpH 9.0MzMw/MnMz/MwToray Research Center, Inc.㼀㻿㻙㻴㻞㻜㻝㻼㻼㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝㻼㼍㼓㼑㻌㻣㻌㻛㻞㻢



# CBI CBITable 6 Molecular Weight DataSample : FMN-809 (Original,Run 1) Mn : 13800File : A08.MWD Mw : 39700Area : 282.24 mV・sec Mz : 66500Operator: HamashigeMv :Date : 2012/7/15 Mw/Mn: 2.88Mz/Mw: 1.68S506744Retention Molecular Intensity d(Weight)Weight %Time Weight (mV) d Log(MW)(min)19.81 353253 0.000 0.000 100.0019.84 344508 0.001 0.001 100.0019.88 335984 0.003 0.002 100.0019.91 327679 0.004 0.003 100.0019.94 319584 0.006 0.004 99.9919.98 311696 0.006 0.004 99.9920.01 304007 0.007 0.005 99.9820.04 296514 0.009 0.006 99.9820.08 289211 0.010 0.007 99.9720.11 282093 0.012 0.008 99.9620.14 275155 0.015 0.010 99.9520.18 268394 0.018 0.012 99.9420.21 261803 0.021 0.014 99.9220.24 255378 0.024 0.016 99.9120.28 249116 0.027 0.017 99.8920.31 243013 0.031 0.020 99.8720.34 237063 0.034 0.022 99.8520.38 231263 0.038 0.025 99.8220.41 225609 0.042 0.028 99.7920.44 220097 0.047 0.031 99.7620.48 214725 0.052 0.034 99.7220.51 209486 0.057 0.038 99.6820.54 204380 0.063 0.042 99.6420.58 199402 0.070 0.046 99.5920.61 194549 0.076 0.050 99.5420.64 189817 0.083 0.055 99.4820.68 185204 0.091 0.060 99.4220.71 180706 0.099 0.066 99.3520.74 176321 0.108 0.072 99.2720.78 172045 0.119 0.079 99.1920.81 167877 0.128 0.085 99.1020.84 163812 0.138 0.092 99.0120.87 159848 0.150 0.100 98.9020.91 155983 0.161 0.107 98.7920.94 152215 0.175 0.117 98.6720.97 148540 0.188 0.125 98.5421.01 144957 0.202 0.135 98.4021.04 141463 0.217 0.145 98.2521.07 138055 0.233 0.156 98.0821.11 134732 0.249 0.167 97.9121.14 131492 0.266 0.178 97.7321.17 128331 0.284 0.191 97.53Table 6 Page - 1File : A08.MWD2S507150S506744Retention Molecular Intensity d(Weight)Weight %Time Weight (mV) d Log(MW)(min)21.21 125249 0.303 0.204 97.3221.24 122243 0.323 0.217 97.0921.27 119312 0.344 0.231 96.8521.31 116453 0.366 0.246 96.6021.34 113664 0.388 0.261 96.3221.37 110945 0.411 0.277 96.0421.41 108292 0.436 0.294 95.7321.44 105705 0.461 0.311 95.4121.47 103181 0.487 0.329 95.0721.51 100719 0.515 0.348 94.7121.54 98318 0.543 0.367 94.3321.57 95976 0.571 0.386 93.9321.61 93692 0.600 0.407 93.5121.64 91463 0.631 0.428 93.0721.67 89289 0.663 0.449 92.6121.71 87168 0.694 0.471 92.1221.74 85100 0.726 0.493 91.6121.77 83082 0.759 0.516 91.0821.81 81113 0.792 0.539 90.5321.84 79192 0.827 0.563 89.9521.87 77318 0.862 0.587 89.3421.91 75490 0.896 0.611 88.7121.94 73706 0.932 0.636 88.0621.97 71966 0.966 0.660 87.3822.01 70268 1.002 0.685 86.6822.04 68612 1.038 0.710 85.9522.07 66995 1.073 0.734 85.2022.11 65418 1.110 0.760 84.4222.14 63879 1.146 0.786 83.6122.17 62378 1.183 0.812 82.7822.21 60913 1.219 0.836 81.9222.24 59483 1.256 0.863 81.0422.27 58088 1.291 0.887 80.1322.31 56727 1.327 0.913 79.2022.34 55398 1.362 0.938 78.2422.37 54102 1.397 0.963 77.2622.41 52837 1.432 0.988 76.2522.44 51602 1.467 1.012 75.2122.47 50398 1.499 1.035 74.1622.51 49222 1.530 1.058 73.0822.54 48074 1.563 1.081 71.9822.57 46954 1.592 1.102 70.8622.61 45861 1.621 1.123 69.7122.64 44794 1.649 1.143 68.5522.67 43753 1.675 1.162 67.3722.71 42737 1.700 1.180 66.1722.74 41745 1.725 1.198 64.9522.77 40777 1.745 1.214 63.7222.81 39832 1.766 1.229 62.47Table 6 Page - 2File : A08.MWDS507150㼀㻿㻙㻴㻞㻜㻝㻼㻼㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝㻼㼍㼓㼑㻌㻤㻌㻛㻞㻢CBICBIS506744Retention Molecular Intensity d(Weight)Weight %Time Weight (mV) d Log(MW)(min)22.84 38909 1.783 1.242 61.2122.87 38009 1.797 1.252 59.9422.91 37130 1.809 1.262 58.6622.94 36272 1.819 1.269 57.3722.97 35434 1.825 1.275 56.0823.01 34617 1.830 1.279 54.7923.04 33819 1.831 1.281 53.4923.07 33040 1.830 1.281 52.1923.11 32279 1.826 1.280 50.9023.14 31537 1.818 1.275 49.6123.17 30812 1.808 1.269 48.3323.21 30104 1.795 1.260 47.0523.24 29413 1.779 1.250 45.7923.27 28738 1.760 1.237 44.5423.31 28080 1.738 1.223 43.3123.34 27437 1.714 1.207 42.0923.37 26809 1.686 1.188 40.8923.41 26196 1.657 1.168 39.7123.44 25597 1.625 1.147 38.5523.47 25013 1.591 1.124 37.4223.51 24442 1.555 1.099 36.3123.54 23885 1.519 1.075 35.2323.57 23340 1.480 1.048 34.1723.61 22809 1.441 1.021 33.1523.64 22290 1.401 0.993 32.1523.67 21783 1.359 0.964 31.1823.71 21289 1.318 0.936 30.2423.74 20805 1.277 0.908 29.3223.77 20333 1.236 0.879 28.4423.81 19873 1.196 0.851 27.5923.84 19423 1.156 0.824 26.7623.87 18983 1.117 0.796 25.9623.91 18554 1.081 0.771 25.1923.94 18134 1.045 0.746 24.4423.97 17725 1.011 0.722 23.7224.01 17325 0.978 0.699 23.0224.04 16934 0.947 0.678 22.3524.07 16553 0.919 0.658 21.6924.11 16180 0.891 0.638 21.0624.14 15816 0.866 0.621 20.4424.17 15461 0.841 0.604 19.8424.21 15113 0.820 0.589 19.2524.24 14774 0.798 0.574 18.6824.27 14443 0.779 0.560 18.1324.31 14119 0.761 0.547 17.5924.34 13803 0.741 0.534 17.0624.37 13494 0.724 0.522 16.5424.41 13192 0.708 0.510 16.0424.44 12897 0.692 0.499 15.55Table 6 Page - 3File : A08.MWDS507150S506744Retention Molecular Intensity d(Weight)Weight %Time Weight (mV) d Log(MW)(min)24.47 12609 0.675 0.488 15.0724.51 12328 0.659 0.477 14.6024.54 12053 0.643 0.465 14.1424.57 11784 0.626 0.453 13.6924.61 11522 0.609 0.441 13.2624.64 11266 0.592 0.429 12.8424.67 11015 0.575 0.417 12.4324.71 10771 0.558 0.406 12.0324.74 10531 0.542 0.394 11.6424.77 10298 0.525 0.382 11.2624.81 10070 0.508 0.370 10.9024.84 9847 0.490 0.357 10.5524.87 9629 0.474 0.346 10.2124.91 9416 0.456 0.333 9.8924.94 9208 0.441 0.322 9.5724.97 9004 0.424 0.310 9.2725.01 8806 0.408 0.298 8.9825.04 8611 0.393 0.287 8.7025.07 8422 0.377 0.276 8.4325.11 8236 0.361 0.265 8.1725.14 8055 0.347 0.255 7.9225.17 7878 0.332 0.244 7.6825.21 7705 0.317 0.233 7.4525.24 7536 0.303 0.223 7.2425.27 7370 0.291 0.214 7.0325.31 7209 0.277 0.204 6.8325.34 7051 0.266 0.196 6.6425.37 6897 0.254 0.187 6.4625.41 6746 0.242 0.179 6.2825.44 6598 0.232 0.171 6.1225.47 6454 0.223 0.165 5.9625.51 6313 0.212 0.157 5.8125.54 6176 0.202 0.150 5.6625.57 6041 0.193 0.143 5.5225.61 5910 0.184 0.136 5.3925.64 5781 0.174 0.129 5.2625.67 5655 0.166 0.124 5.1525.71 5532 0.158 0.117 5.0325.74 5412 0.151 0.112 4.9225.77 5295 0.145 0.108 4.8225.81 5180 0.138 0.102 4.7225.84 5068 0.132 0.098 4.6325.87 4958 0.125 0.093 4.5425.91 4851 0.119 0.089 4.4525.94 4746 0.113 0.084 4.3725.97 4644 0.108 0.081 4.2926.01 4543 0.103 0.077 4.2226.04 4445 0.096 0.072 4.1526.07 4350 0.093 0.070 4.08Table 6 Page - 4File : A08.MWDS507150㼀㻿㻙㻴㻞㻜㻝㻼㻼㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝㻼㼍㼓㼑㻌㻥㻌㻛㻞㻢S506744Retention Molecular Intensity d(Weight)Weight %Time Weight (mV) d Log(MW)(min)26.11 4256 0.089 0.066 4.0226.14 4164 0.084 0.063 3.9626.17 4075 0.080 0.060 3.9026.21 3987 0.077 0.058 3.8526.24 3902 0.073 0.055 3.8026.27 3818 0.070 0.052 3.7526.31 3736 0.066 0.050 3.7026.34 3656 0.063 0.048 3.6526.37 3578 0.060 0.045 3.6126.41 3501 0.057 0.043 3.5726.44 3426 0.054 0.041 3.5326.47 3353 0.052 0.039 3.4926.51 3282 0.050 0.037 3.4626.54 3212 0.047 0.036 3.4226.57 3143 0.045 0.034 3.3926.61 3076 0.044 0.034 3.3626.64 3011 0.043 0.032 3.3326.67 2947 0.041 0.031 3.3026.71 2884 0.040 0.030 3.2726.74 2823 0.039 0.030 3.2426.77 2763 0.039 0.029 3.2226.81 2705 0.038 0.029 3.1926.84 2647 0.036 0.028 3.1626.87 2591 0.036 0.027 3.1426.91 2536 0.035 0.027 3.1126.94 2483 0.036 0.027 3.0926.97 2430 0.036 0.027 3.0627.01 2379 0.036 0.027 3.0427.04 2329 0.035 0.027 3.0127.07 2280 0.036 0.027 2.9927.11 2232 0.036 0.028 2.9627.14 2185 0.036 0.028 2.9427.17 2139 0.038 0.029 2.9127.21 2094 0.039 0.030 2.8827.24 2050 0.042 0.032 2.8527.27 2007 0.042 0.032 2.8227.31 1965 0.043 0.033 2.7927.34 1924 0.044 0.034 2.7627.37 1883 0.045 0.035 2.7327.41 1844 0.046 0.035 2.7027.44 1805 0.047 0.036 2.6627.47 1768 0.048 0.037 2.6327.51 1731 0.049 0.038 2.6027.54 1695 0.050 0.039 2.5627.57 1659 0.050 0.039 2.5327.61 1625 0.051 0.040 2.4927.64 1591 0.052 0.040 2.4527.67 1558 0.053 0.041 2.4227.71 1526 0.054 0.042 2.38Table 6 Page - 5File : A08.MWDS507150S506744Retention Molecular Intensity d(Weight)Weight %Time Weight (mV) d Log(MW)(min)27.74 1494 0.054 0.042 2.3427.77 1463 0.055 0.043 2.3027.81 1433 0.058 0.045 2.2627.84 1403 0.060 0.047 2.2227.87 1374 0.063 0.049 2.1727.91 1345 0.066 0.052 2.1327.94 1318 0.072 0.056 2.0827.97 1290 0.079 0.062 2.0228.01 1264 0.086 0.067 1.9628.04 1238 0.096 0.075 1.9028.07 1212 0.107 0.084 1.8228.11 1187 0.117 0.092 1.7428.14 1163 0.127 0.099 1.6528.17 1139 0.134 0.105 1.5628.21 1115 0.138 0.109 1.4628.24 1093 0.141 0.111 1.3628.27 1070 0.141 0.111 1.2628.31 1048 0.137 0.108 1.1628.34 1027 0.132 0.104 1.0728.37 1006 0.123 0.097 0.9828.41 985 0.113 0.089 0.9028.44 965 0.103 0.081 0.8328.47 945 0.090 0.071 0.7628.51 926 0.080 0.063 0.7028.54 907 0.070 0.055 0.6528.57 889 0.060 0.047 0.6128.61 870 0.051 0.041 0.5728.64 853 0.044 0.035 0.5428.67 835 0.038 0.030 0.5128.71 818 0.031 0.025 0.4828.74 802 0.027 0.021 0.4628.77 785 0.022 0.018 0.4528.81 770 0.019 0.015 0.4328.84 754 0.017 0.013 0.4228.87 739 0.015 0.012 0.4128.91 724 0.014 0.012 0.4028.94 709 0.013 0.010 0.3928.97 695 0.012 0.009 0.3829.01 681 0.013 0.010 0.3729.04 667 0.012 0.009 0.3729.07 654 0.011 0.009 0.3629.11 640 0.011 0.009 0.3529.14 628 0.012 0.009 0.3429.17 615 0.012 0.010 0.3329.20 603 0.014 0.011 0.3229.24 590 0.016 0.013 0.3129.27 579 0.017 0.014 0.3029.30 567 0.020 0.016 0.2929.34 556 0.022 0.018 0.27Table 6 Page - 6File : A08.MWDS507150㼀㻿㻙㻴㻞㻜㻝㻼㻼㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝㻼㼍㼓㼑㻌㻝㻜㻌㻛㻞㻢S506744Retention Molecular Intensity d(Weight)Weight %Time Weight (mV) d Log(MW)(min)29.37 544 0.024 0.019 0.2629.40 534 0.027 0.022 0.2429.44 523 0.029 0.023 0.2229.47 512 0.030 0.024 0.2029.50 502 0.030 0.024 0.1729.54 492 0.030 0.024 0.1529.57 482 0.029 0.024 0.1329.60 473 0.027 0.022 0.1129.64 463 0.024 0.019 0.0929.67 454 0.022 0.018 0.0829.70 445 0.020 0.016 0.0729.74 436 0.016 0.013 0.0529.77 428 0.014 0.011 0.0429.80 419 0.011 0.009 0.0329.84 411 0.009 0.007 0.0329.87 403 0.008 0.006 0.0229.90 395 0.006 0.005 0.0229.94 387 0.005 0.004 0.0129.97 379 0.004 0.004 0.0130.00 372 0.004 0.003 0.0130.04 364 0.003 0.003 0.0130.07 357 0.003 0.002 0.0030.10 350 0.002 0.002 0.0030.14 343 0.001 0.001 0.0030.17 337 0.001 0.001 0.0030.20 330 0.000 0.000 0.0030.24 323 0.001 0.000 0.0030.27 317 0.000 0.000 0.0030.30 311 0.000 0.000 0.0030.34 305 0.000 0.000 0.0030.37 299 0.000 0.000 0.00Table 6 Page - 7File : A08.MWDS507150㼀㻿㻙㻴㻞㻜㻝㻼㻼㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝㻼㼍㼓㼑㻌㻝㻝㻌㻛㻞㻢Table 7 Molecular Weight DataSample : FMN-809 (Original,Run 2) Mn : 14200File : A09.MWD Mw : 39800Area : 281.48 mV・sec Mz : 67200Operator: HamashigeMv :Date : 2012/7/15 Mw/Mn: 2.80Mz/Mw: 1.69S506744Retention Molecular Intensity d(Weight)Weight %Time Weight (mV) d Log(MW)(min)19.81 353253 0.000 0.000 100.0019.84 344508 0.002 0.001 100.0019.88 335984 0.003 0.002 100.0019.91 327679 0.005 0.003 99.9919.94 319584 0.006 0.004 99.9919.98 311696 0.008 0.005 99.9820.01 304007 0.009 0.006 99.9820.04 296514 0.011 0.007 99.9720.08 289211 0.013 0.009 99.9620.11 282093 0.014 0.010 99.9520.14 275155 0.017 0.011 99.9420.18 268394 0.019 0.013 99.9320.21 261803 0.022 0.014 99.9120.24 255378 0.026 0.017 99.8920.28 249116 0.029 0.019 99.8720.31 243013 0.032 0.021 99.8520.34 237063 0.036 0.024 99.8320.38 231263 0.041 0.027 99.8020.41 225609 0.046 0.030 99.7720.44 220097 0.050 0.033 99.7320.48 214725 0.055 0.037 99.7020.51 209486 0.060 0.040 99.6520.54 204380 0.067 0.044 99.6120.58 199402 0.072 0.048 99.5620.61 194549 0.080 0.053 99.5020.64 189817 0.087 0.058 99.4420.68 185204 0.095 0.063 99.3720.71 180706 0.104 0.069 99.3020.74 176321 0.113 0.075 99.2220.78 172045 0.122 0.082 99.1420.81 167877 0.132 0.088 99.0520.84 163812 0.143 0.095 98.9520.87 159848 0.154 0.103 98.8420.91 155983 0.166 0.111 98.7220.94 152215 0.180 0.120 98.6020.97 148540 0.193 0.130 98.4621.01 144957 0.207 0.139 98.3221.04 141463 0.222 0.149 98.1621.07 138055 0.237 0.159 98.0021.11 134732 0.253 0.170 97.8221.14 131492 0.270 0.182 97.6321.17 128331 0.289 0.194 97.43Table 7 Page - 1File : A09.MWD3S507150S506744Retention Molecular Intensity d(Weight)Weight %Time Weight (mV) d Log(MW)(min)21.21 125249 0.308 0.207 97.2221.24 122243 0.328 0.221 96.9921.27 119312 0.349 0.235 96.7421.31 116453 0.370 0.250 96.4821.34 113664 0.392 0.265 96.2121.37 110945 0.415 0.280 95.9221.41 108292 0.439 0.297 95.6121.44 105705 0.464 0.314 95.2921.47 103181 0.490 0.332 94.9421.51 100719 0.517 0.350 94.5821.54 98318 0.544 0.369 94.2021.57 95976 0.572 0.388 93.8021.61 93692 0.600 0.408 93.3821.64 91463 0.630 0.428 92.9321.67 89289 0.660 0.449 92.4721.71 87168 0.692 0.471 91.9921.74 85100 0.723 0.492 91.4821.77 83082 0.755 0.515 90.9521.81 81113 0.789 0.538 90.3921.84 79192 0.823 0.562 89.8221.87 77318 0.857 0.586 89.2121.91 75490 0.890 0.608 88.5921.94 73706 0.926 0.633 87.9421.97 71966 0.961 0.658 87.2622.01 70268 0.998 0.684 86.5622.04 68612 1.033 0.708 85.8322.07 66995 1.068 0.733 85.0822.11 65418 1.104 0.758 84.3022.14 63879 1.140 0.783 83.5022.17 62378 1.175 0.808 82.6722.21 60913 1.210 0.833 81.8222.24 59483 1.246 0.858 80.9422.27 58088 1.281 0.883 80.0322.31 56727 1.316 0.908 79.1022.34 55398 1.351 0.933 78.1522.37 54102 1.385 0.957 77.1722.41 52837 1.419 0.981 76.1722.44 51602 1.452 1.005 75.1522.47 50398 1.485 1.028 74.1022.51 49222 1.516 1.051 73.0322.54 48074 1.547 1.073 71.9322.57 46954 1.578 1.095 70.8222.61 45861 1.607 1.116 69.6822.64 44794 1.634 1.136 68.5322.67 43753 1.660 1.154 67.3522.71 42737 1.684 1.172 66.1622.74 41745 1.707 1.189 64.9522.77 40777 1.729 1.205 63.7322.81 39832 1.748 1.219 62.49Table 7 Page - 2File : A09.MWDS507150㼀㻿㻙㻴㻞㻜㻝㻼㻼㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝㻼㼍㼓㼑㻌㻝㻞㻌㻛㻞㻢CBICBIS506744Retention Molecular Intensity d(Weight)Weight %Time Weight (mV) d Log(MW)(min)22.84 38909 1.764 1.231 61.2422.87 38009 1.779 1.243 59.9822.91 37130 1.791 1.252 58.7122.94 36272 1.801 1.260 57.4322.97 35434 1.808 1.266 56.1523.01 34617 1.812 1.270 54.8623.04 33819 1.815 1.273 53.5723.07 33040 1.812 1.272 52.2923.11 32279 1.807 1.270 51.0023.14 31537 1.800 1.265 49.7223.17 30812 1.790 1.259 48.4523.21 30104 1.777 1.251 47.1823.24 29413 1.762 1.242 45.9323.27 28738 1.743 1.229 44.6923.31 28080 1.721 1.214 43.4623.34 27437 1.697 1.198 42.2523.37 26809 1.671 1.181 41.0623.41 26196 1.641 1.161 39.8923.44 25597 1.611 1.140 38.7423.47 25013 1.579 1.118 37.6123.51 24442 1.543 1.094 36.5123.54 23885 1.507 1.069 35.4323.57 23340 1.468 1.042 34.3823.61 22809 1.430 1.016 33.3623.64 22290 1.391 0.989 32.3723.67 21783 1.351 0.961 31.4023.71 21289 1.310 0.933 30.4623.74 20805 1.270 0.905 29.5523.77 20333 1.229 0.877 28.6723.81 19873 1.190 0.849 27.8223.84 19423 1.151 0.822 26.9923.87 18983 1.113 0.796 26.2023.91 18554 1.076 0.770 25.4323.94 18134 1.042 0.746 24.6823.97 17725 1.008 0.722 23.9624.01 17325 0.976 0.700 23.2624.04 16934 0.945 0.678 22.5824.07 16553 0.917 0.658 21.9324.11 16180 0.891 0.640 21.2924.14 15816 0.867 0.623 20.6724.17 15461 0.842 0.606 20.0724.21 15113 0.820 0.590 19.4824.24 14774 0.799 0.576 18.9124.27 14443 0.779 0.562 18.3524.31 14119 0.760 0.549 17.8124.34 13803 0.743 0.536 17.2824.37 13494 0.725 0.524 16.7624.41 13192 0.709 0.513 16.2624.44 12897 0.693 0.502 15.76Table 7 Page - 3File : A09.MWDS507150S506744Retention Molecular Intensity d(Weight)Weight %Time Weight (mV) d Log(MW)(min)24.47 12609 0.678 0.491 15.2824.51 12328 0.663 0.480 14.8024.54 12053 0.647 0.469 14.3424.57 11784 0.632 0.459 13.8924.61 11522 0.616 0.448 13.4524.64 11266 0.600 0.436 13.0224.67 11015 0.583 0.424 12.6024.71 10771 0.566 0.412 12.2024.74 10531 0.549 0.400 11.8124.77 10298 0.533 0.388 11.4224.81 10070 0.516 0.377 11.0624.84 9847 0.500 0.365 10.7024.87 9629 0.483 0.353 10.3524.91 9416 0.465 0.340 10.0224.94 9208 0.449 0.329 9.7024.97 9004 0.434 0.318 9.3825.01 8806 0.418 0.307 9.0925.04 8611 0.402 0.295 8.8025.07 8422 0.387 0.284 8.5225.11 8236 0.371 0.273 8.2525.14 8055 0.358 0.263 8.0025.17 7878 0.342 0.251 7.7525.21 7705 0.328 0.242 7.5225.24 7536 0.314 0.232 7.2925.27 7370 0.302 0.222 7.0725.31 7209 0.289 0.213 6.8725.34 7051 0.277 0.205 6.6725.37 6897 0.265 0.196 6.4825.41 6746 0.254 0.188 6.2925.44 6598 0.243 0.180 6.1225.47 6454 0.233 0.173 5.9525.51 6313 0.223 0.165 5.7925.54 6176 0.213 0.158 5.6425.57 6041 0.204 0.152 5.4925.61 5910 0.196 0.145 5.3525.64 5781 0.187 0.139 5.2225.67 5655 0.178 0.133 5.0925.71 5532 0.171 0.127 4.9725.74 5412 0.162 0.121 4.8525.77 5295 0.155 0.116 4.7425.81 5180 0.149 0.111 4.6325.84 5068 0.141 0.106 4.5325.87 4958 0.135 0.101 4.4325.91 4851 0.129 0.097 4.3425.94 4746 0.123 0.092 4.2525.97 4644 0.118 0.088 4.1726.01 4543 0.113 0.084 4.0926.04 4445 0.108 0.081 4.0126.07 4350 0.102 0.076 3.93Table 7 Page - 4File : A09.MWDS507150㼀㻿㻙㻴㻞㻜㻝㻼㻼㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝㻼㼍㼓㼑㻌㻝㻟㻌㻛㻞㻢S506744Retention Molecular Intensity d(Weight)Weight %Time Weight (mV) d Log(MW)(min)26.11 4256 0.098 0.073 3.8626.14 4164 0.093 0.070 3.8026.17 4075 0.089 0.067 3.7326.21 3987 0.084 0.064 3.6726.24 3902 0.081 0.061 3.6126.27 3818 0.078 0.059 3.5626.31 3736 0.074 0.056 3.5026.34 3656 0.069 0.052 3.4526.37 3578 0.066 0.050 3.4126.41 3501 0.064 0.048 3.3626.44 3426 0.061 0.046 3.3226.47 3353 0.057 0.043 3.2826.51 3282 0.056 0.042 3.2426.54 3212 0.053 0.040 3.2026.57 3143 0.051 0.039 3.1626.61 3076 0.049 0.037 3.1326.64 3011 0.047 0.035 3.0926.67 2947 0.045 0.035 3.0626.71 2884 0.044 0.034 3.0326.74 2823 0.042 0.032 3.0026.77 2763 0.041 0.031 2.9726.81 2705 0.040 0.031 2.9426.84 2647 0.040 0.030 2.9126.87 2591 0.039 0.030 2.8826.91 2536 0.038 0.029 2.8626.94 2483 0.037 0.029 2.8326.97 2430 0.037 0.028 2.8027.01 2379 0.037 0.028 2.7827.04 2329 0.037 0.028 2.7527.07 2280 0.037 0.028 2.7227.11 2232 0.037 0.028 2.7027.14 2185 0.037 0.029 2.6727.17 2139 0.038 0.029 2.6527.21 2094 0.038 0.030 2.6227.24 2050 0.039 0.030 2.5927.27 2007 0.040 0.031 2.5627.31 1965 0.040 0.031 2.5327.34 1924 0.042 0.032 2.5027.37 1883 0.043 0.033 2.4727.41 1844 0.044 0.034 2.4427.44 1805 0.044 0.034 2.4127.47 1768 0.045 0.035 2.3827.51 1731 0.045 0.035 2.3527.54 1695 0.046 0.036 2.3227.57 1659 0.047 0.037 2.2827.61 1625 0.047 0.037 2.2527.64 1591 0.048 0.037 2.2127.67 1558 0.049 0.038 2.1827.71 1526 0.049 0.038 2.15Table 7 Page - 5File : A09.MWDS507150S506744Retention Molecular Intensity d(Weight)Weight %Time Weight (mV) d Log(MW)(min)27.74 1494 0.049 0.038 2.1127.77 1463 0.050 0.039 2.0727.81 1433 0.051 0.040 2.0427.84 1403 0.054 0.042 2.0027.87 1374 0.057 0.044 1.9627.91 1345 0.061 0.048 1.9227.94 1318 0.067 0.052 1.8727.97 1290 0.073 0.057 1.8228.01 1264 0.080 0.063 1.7728.04 1238 0.089 0.070 1.7128.07 1212 0.099 0.078 1.6428.11 1187 0.108 0.085 1.5628.14 1163 0.117 0.092 1.4828.17 1139 0.124 0.097 1.3928.21 1115 0.130 0.102 1.3028.24 1093 0.132 0.104 1.2128.27 1070 0.133 0.105 1.1128.31 1048 0.130 0.103 1.0228.34 1027 0.124 0.098 0.9328.37 1006 0.116 0.092 0.8528.41 985 0.105 0.083 0.7728.44 965 0.095 0.075 0.7028.47 945 0.084 0.067 0.6428.51 926 0.074 0.058 0.5928.54 907 0.063 0.050 0.5428.57 889 0.054 0.043 0.5028.61 870 0.046 0.036 0.4728.64 853 0.038 0.031 0.4428.67 835 0.032 0.025 0.4128.71 818 0.028 0.022 0.3928.74 802 0.023 0.018 0.3828.77 785 0.018 0.015 0.3628.81 770 0.015 0.012 0.3528.84 754 0.013 0.011 0.3428.87 739 0.011 0.009 0.3328.91 724 0.010 0.008 0.3328.94 709 0.009 0.007 0.3228.97 695 0.007 0.006 0.3129.01 681 0.006 0.005 0.3129.04 667 0.006 0.005 0.3129.07 654 0.006 0.005 0.3029.11 640 0.007 0.005 0.3029.14 628 0.007 0.006 0.2929.17 615 0.008 0.007 0.2929.20 603 0.010 0.008 0.2829.24 590 0.013 0.011 0.2729.27 579 0.016 0.013 0.2629.30 567 0.018 0.015 0.2529.34 556 0.020 0.016 0.23Table 7 Page - 6File : A09.MWDS507150㼀㻿㻙㻴㻞㻜㻝㻼㻼㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝㻼㼍㼓㼑㻌㻝㻠㻌㻛㻞㻢S506744Retention Molecular Intensity d(Weight)Weight %Time Weight (mV) d Log(MW)(min)29.37 544 0.022 0.018 0.2229.40 534 0.022 0.018 0.2029.44 523 0.025 0.020 0.1829.47 512 0.027 0.022 0.1729.50 502 0.028 0.023 0.1529.54 492 0.027 0.022 0.1329.57 482 0.026 0.021 0.1129.60 473 0.025 0.021 0.0929.64 463 0.023 0.018 0.0729.67 454 0.020 0.016 0.0629.70 445 0.017 0.014 0.0529.74 436 0.014 0.012 0.0429.77 428 0.011 0.009 0.0329.80 419 0.008 0.006 0.0229.84 411 0.006 0.005 0.0229.87 403 0.006 0.005 0.0129.90 395 0.005 0.004 0.0129.94 387 0.004 0.003 0.0129.97 379 0.003 0.003 0.0030.00 372 0.002 0.002 0.0030.04 364 0.002 0.001 0.0030.07 357 0.001 0.001 0.0030.10 350 0.000 0.000 0.0030.14 343 0.000 0.000 0.0030.17 337 0.000 0.000 0.0030.20 330 0.000 0.000 0.0030.24 323 0.000 0.000 0.0030.27 317 0.000 0.000 0.0030.30 311 0.000 0.000 0.0030.34 305 0.000 0.000 0.0030.37 299 0.000 0.000 0.00Table 7 Page - 7File : A09.MWDS507150㼀㻿㻙㻴㻞㻜㻝㻼㻼㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝㻼㼍㼓㼑㻌㻝㻡㻌㻛㻞㻢

## 15 20 25 30 35 40 0 1 2 3 4 5 6 7 8 Date : Column : Operator : Apparatus : Temperature : Injection Volume : Detector : Concentration : Flow Rate : Sample : Solvent :

2012/07/15 TSKgel GMHXL(2),G2500HXL(1) Hamashige GPC-MALLS(1) 23.0 °C 0.200 mL RI 32x 0.20 % 1.0 mL/min FMN-809 (Original,Run 1) THF 

# Fig . 2-1 Gel Permeation Chromatogram

Toray Research Center Inc. 

## F ile: A 0 8



# Retention Time (min)RIIntensitymV 

# 1

15 20 25 30 35 40 0 1 2 3 4 5 6 7 8 Date : Column : Operator : Apparatus : Temperature : Injection Volume : Detector : Concentration : Flow Rate : Sample : Solvent :

2012/07/15 TSKgel GMHXL(2),G2500HXL(1) Hamashige GPC-MALLS(1) 23.0 °C 0.200 mL RI 32x 0.20 % 1.0 mL/min FMN-809 (Original,Run 2) THF 

# Fig . 2-2 Gel Permeation Chromatogram

Toray Research Center Inc. 

## F ile: A 0 9



# Retention Time (min)RIIntensitymV 

# 2 㼀㻿㻙㻴㻞㻜㻝㻼㻼 㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝

㻼㼍㼓㼑㻌㻝㻢㻌㻛㻞㻢



# CBI

CBI

## 15 20 25 30 35 40 0 1 2 3 4 5 6 7 8 Date : Column : Operator : Apparatus : Temperature : Injection Volume : Detector : Concentration : Flow Rate : Sample : Solvent :

2012/07/15 TSKgel GMHXL(2),G2500HXL(1) Hamashige GPC-MALLS(1) 23.0 °C 0.200 mL RI 32x 0.20 % 1.0 mL/min FMN-809 (pH 1.2-1) THF 

# Fig . 2-3 Gel Permeation Chromatogram

Toray Research Center Inc. 

## F ile: A 1 1



# Retention Time (min)RIIntensitymV 

# 3

15 20 25 30 35 40 0 1 2 3 4 5 6 7 8 Date : Column : Operator : Apparatus : Temperature : Injection Volume : Detector : Concentration : Flow Rate : Sample : Solvent :

2012/07/15 TSKgel GMHXL(2),G2500HXL(1) Hamashige GPC-MALLS(1) 23.0 °C 0.200 mL RI 32x 0.20 % 1.0 mL/min FMN-809 (pH 1.2-2) THF 

# Fig . 2-4 Gel Permeation Chromatogram

Toray Research Center Inc. 

## F ile: A 2 3



# Retention Time (min)RIIntensitymV 

# 4 㼀㻿㻙㻴㻞㻜㻝㻼㻼 㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝

㻼㼍㼓㼑㻌㻝㻣㻌㻛㻞㻢



# CBI

CBI

## 15 20 25 30 35 40 0 1 2 3 4 5 6 7 8 Date : Column : Operator : Apparatus : Temperature : Injection Volume : Detector : Concentration : Flow Rate : Sample : Solvent :

2012/07/15 TSKgel GMHXL(2),G2500HXL(1) Hamashige GPC-MALLS(1) 23.0 °C 0.200 mL RI 32x 0.20 % 1.0 mL/min FMN-809 (pH 4.0-1) THF 

# Fig . 2-5 Gel Permeation Chromatogram

Toray Research Center Inc. 

## F ile: A 1 2



# Retention Time (min)RIIntensitymV 

# 5

15 20 25 30 35 40 0 1 2 3 4 5 6 7 8 Date : Column : Operator : Apparatus : Temperature : Injection Volume : Detector : Concentration : Flow Rate : Sample : Solvent :

2012/07/15 TSKgel GMHXL(2),G2500HXL(1) Hamashige GPC-MALLS(1) 23.0 °C 0.200 mL RI 32x 0.20 % 1.0 mL/min FMN-809 (pH 4.0-2) THF 

# Fig . 2-6 Gel Permeation Chromatogram

Toray Research Center Inc. 

## F ile: A 2 4



# Retention Time (min)RIIntensitymV 

# 6 㼀㻿㻙㻴㻞㻜㻝㻼㻼 㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝

㻼㼍㼓㼑㻌㻝㻤㻌㻛㻞㻢



# CBI

CBI

## 15 20 25 30 35 40 0 1 2 3 4 5 6 7 8 Date : Column : Operator : Apparatus : Temperature : Injection Volume : Detector : Concentration : Flow Rate : Sample : Solvent :

2012/07/15 TSKgel GMHXL(2),G2500HXL(1) Hamashige GPC-MALLS(1) 23.0 °C 0.200 mL RI 32x 0.20 % 1.0 mL/min FMN-809 (pH 7.0-1) THF 

# Fig . 2-7 Gel Permeation Chromatogram

Toray Research Center Inc. 

## F ile: A 1 3



# Retention Time (min)RIIntensitymV 

# 7

15 20 25 30 35 40 0 1 2 3 4 5 6 7 8 Date : Column : Operator : Apparatus : Temperature : Injection Volume : Detector : Concentration : Flow Rate : Sample : Solvent :

2012/07/15 TSKgel GMHXL(2),G2500HXL(1) Hamashige GPC-MALLS(1) 23.0 °C 0.200 mL RI 32x 0.20 % 1.0 mL/min FMN-809 (pH 7.0-2) THF 

# Fig . 2-8 Gel Permeation Chromatogram

Toray Research Center Inc. 

## F ile: A 1 7



# Retention Time (min)RIIntensitymV 

# 8 㼀㻿㻙㻴㻞㻜㻝㻼㻼 㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝

㻼㼍㼓㼑㻌㻝㻥㻌㻛㻞㻢



# CBI

CBI

## 15 20 25 30 35 40 0 1 2 3 4 5 6 7 8 Date : Column : Operator : Apparatus : Temperature : Injection Volume : Detector : Concentration : Flow Rate : Sample : Solvent :

2012/07/15 TSKgel GMHXL(2),G2500HXL(1) Hamashige GPC-MALLS(1) 23.0 °C 0.200 mL RI 32x 0.20 % 1.0 mL/min FMN-809 (pH 9.0-1) THF 

# Fig . 2-9 Gel Permeation Chromatogram

Toray Research Center Inc. 

## F ile: A 1 4



# Retention Time (min)RIIntensitymV 

# 9

15 20 25 30 35 40 0 1 2 3 4 5 6 7 8 Date : Column : Operator : Apparatus : Temperature : Injection Volume : Detector : Concentration : Flow Rate : Sample : Solvent :

2012/07/15 TSKgel GMHXL(2),G2500HXL(1) Hamashige GPC-MALLS(1) 23.0 °C 0.200 mL RI 32x 0.20 % 1.0 mL/min FMN-809 (pH 9.0-2) THF 

# Fig . 2-10 Gel Permeation Chromatogram

Toray Research Center Inc. 

## F ile: A 1 8



# Retention Time (min)RIIntensitymV 

# 10 㼀㻿㻙㻴㻞㻜㻝㻼㻼 㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝

㻼㼍㼓㼑㻌㻞㻜㻌㻛㻞㻢



# CBI

CBI

## 15 20 25 30 35 40 0 1 2 3 4 5 6 7 8 Date : C olumn : Operator : Apparatus : Temperature : Inj ection Volume : Detector : C oncentration : Flow Rate : Sample : Solvent :

2012/07/15 TSK gel G MHX L(2),G 2500HX L(1) Hamashige G PC

-MALLS(1) 23.0 °C 0.200 mL RI 32x - 1.0 mL/min Solvent THF 

# Fig . 2-11 Gel Permeation Chromatogram

Toray Research Center Inc. 

## File: A22



# Retention Time (min)RIIntensitymV 

# 11

18 20 22 24 26 28 30 32 0 1 2 3 4 5 6 7 8 9 10 Flow Rate : Solvent : Date : C olumn : Operator : Apparatus : Temperature : Sample : Detector :

1.0 mL/min THF 2012/07/15 TSK gel G MHX L(2),G 2500HX L(1) Hamashige G PC

-MALLS(1) 23.0 °C Polystyrene RI 32x 

# Fig . 2-12 Calibration Curve

Toray Research Center Inc. 

## F ile: C 0 2

. B X X



# L og( MW)

= A ( 0

) + A ( 1 ) T + A ( 2

) T 2 

# + A ( 3 ) T 3 

# + ・・・ C oef f icien t( O rd er: 3 )

A (

0 ) : 1 3

. 6

6 75

A ( 1 ) : - 0

. 5

0 3 0 5 5 6

A (

2 ) : 5

. 2 1 5 6 9 5 E

- 0 3

A ( 3 ) :

- 2

. 5

8 70

5 7E

- 0 5 Molecular Weight Data

R T( m in

) MW

1 9

. 5

3 4 2 70

0 0

2 0

. 6

8 1 9 0 0 0 0

2 1

. 6 4 9 6 4 0 0

2 2

. 8

4 3 79

0 0

2 3

. 8

7 1 8 1 0

0

2 4

. 71 1 0 2 0 0

2 5

. 5

9 5 9 70

2 7. 0

2 2 6 3 0

2 8

. 3

7 1 0 1 0

2 9

. 1 7 5 9 0



# Retention Time (min)LogMolecularWeight 

# 12 㼀㻿㻙㻴㻞㻜㻝㻼㻼 㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝

㻼㼍㼓㼑㻌㻞㻝㻌㻛㻞㻢



# CBI

CBI

## 10 2 

## 10 3 

## 10 4 

## 10 5 

## 10 6 

## 10 7 

## 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 Mz : Mz/Mw : Mw : Mw/Mn : Date : Mn : Mv : Operator :

66500 1.68 39700 2.88 2012/07/15 13800 Hamashige



# Sample: FMN-809 (Original,Run 1) 

# Fig . 2-13 Molecular Weight Distribution Curve

Toray Research Center Inc. 

## File: A08.MWD



# Molecular WeightdW/dLogM 

# 13

10 2 

## 10 3 

## 10 4 

## 10 5 

## 10 6 

## 10 7 

## 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 Mz : Mz/Mw : Mw : Mw/Mn : Date : Mn : Mv : Operator :

67200 1.69 39800 2.80 2012/07/15 14200 Hamashige



# Sample: FMN-809 (Original,Run 2) 

# Fig . 2-14 Molecular Weight Distribution Curve

Toray Research Center Inc. 

## File: A09.MWD



# Molecular WeightdW/dLogM 

# 14 㼀㻿㻙㻴㻞㻜㻝㻼㻼 㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝

㻼㼍㼓㼑㻌㻞㻞㻌㻛㻞㻢CBICBICBICBICBICBI 

# CBI

CBI

## 10 2 

## 10 3 

## 10 4 

## 10 5 

## 10 6 

## 10 7 

## 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 Mz : Mz/Mw : Mw : Mw/Mn : Date : Mn : Mv : Operator :

66000 1.68 39300 3.05 2012/07/15 12900 Hamashige



# Sample: FMN-809 (pH 1.2-1) 

# Fig . 2-15 Molecular Weight Distribution Curve

Toray Research Center Inc. 

## File: A11.MWD



# Molecular WeightdW/dLogM 

# 15

10 2 

## 10 3 

## 10 4 

## 10 5 

## 10 6 

## 10 7 

## 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 Mz : Mz/Mw : Mw : Mw/Mn : Date : Mn : Mv : Operator :

65100 1.67 38900 3.06 2012/07/15 12700 Hamashige



# Sample: FMN-809 (pH 1.2-2) 

# Fig . 2-16 Molecular Weight Distribution Curve

Toray Research Center Inc. 

## File: A23.MWD



# Molecular WeightdW/dLogM 

# 16 㼀㻿㻙㻴㻞㻜㻝㻼㻼 㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝

㻼㼍㼓㼑㻌㻞㻟㻌㻛㻞㻢CBICBICBICBICBICBI 

# CBI

CBI

## 10 2 

## 10 3 

## 10 4 

## 10 5 

## 10 6 

## 10 7 

## 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 Mz : Mz/Mw : Mw : Mw/Mn : Date : Mn : Mv : Operator :

65700 1.67 39300 3.00 2012/07/15 13100 Hamashige



# Sample: FMN-809 (pH 4.0-1) 

# Fig . 2-17 Molecular Weight Distribution Curve

Toray Research Center Inc. 

## File: A12.MWD



# Molecular WeightdW/dLogM 

# 17

10 2 

## 10 3 

## 10 4 

## 10 5 

## 10 6 

## 10 7 

## 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 Mz : Mz/Mw : M w: M w/ M n : Dat e : M n: M v: O per at or :

65800 1. 67 39400 2. 96 2012/ 07/ 15 13300 Ham ashi ge



# Sample: FMN-809 (pH 4.0-2) 

# Fig . 2-18 Molecular Weight Distribution Curve

Toray Research Center Inc. 

## File: A24.MWD



# Molecular WeightdW/dLogM 

# 18 㼀㻿㻙㻴㻞㻜㻝㻼㻼 㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝

㻼㼍㼓㼑㻌㻞㻠㻌㻛㻞㻢CBICBICBICBICBICBI



# CBI CBI

## 10 2 

## 10 3 

## 10 4 

## 10 5 

## 10 6 

## 10 7 

## 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 Mz : Mz/Mw : Mw : Mw/Mn : Date : Mn : Mv : Operator :

66100 1.68 39300 2.93 2012/07/15 13400 Hamashige



# Sample: FMN-809 (pH 7.0-1) 

# Fig . 2-19 Molecular Weight Distribution Curve

Toray Research Center Inc. 

## File: A13.MWD



# Molecular WeightdW/dLogM 

# 19

10 2 

## 10 3 

## 10 4 

## 10 5 

## 10 6 

## 10 7 

## 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 Mz : Mz/Mw : Mw : Mw/Mn : Date : Mn : Mv : Operator :

66000 1.68 39300 3.00 2012/07/15 13100 Hamashige



# Sample: FMN-809 (pH 7.0-2) 

# Fig . 2-20 Molecular Weight Distribution Curve

Toray Research Center Inc. 

## File: A17.MWD



# Molecular WeightdW/dLogM 

# 20 㼀㻿㻙㻴㻞㻜㻝㻼㻼 㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝

㻼㼍㼓㼑㻌㻞㻡㻌㻛㻞㻢CBICBICBICBICBICBI



# CBI CBI

## 10 2 

## 10 3 

## 10 4 

## 10 5 

## 10 6 

## 10 7 

## 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 Mz : Mz/Mw : Mw : Mw/Mn : Date : Mn : Mv : Operator :

65900 1.69 39100 3.13 2012/07/15 12500 Hamashige



# Sample: FMN-809 (pH 9.0-1) 

# Fig . 2-21 Molecular Weight Distribution Curve

Toray Research Center Inc. 

## File: A14.MWD



# Molecular WeightdW/dLogM 

# 21

10 2 

## 10 3 

## 10 4 

## 10 5 

## 10 6 

## 10 7 

## 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 Mz : Mz/Mw : Mw : Mw/Mn : Date : Mn : Mv : Operator :

66200 1.68 39300 3.02 2012/07/15 13000 Hamashige



# Sample: FMN-809 (pH 9.0-2) 

# Fig . 2-22 Molecular Weight Distribution Curve

Toray Research Center Inc. 

## File: A18.MWD



# Molecular WeightdW/dLogM 

# 22 㼀㻿㻙㻴㻞㻜㻝㻼㻼 㻭㼠㼠㼍㼏㼔㼙㼑㼚㼠㻌㻝

㻼㼍㼓㼑㻌㻞㻢㻌㻛㻞㻢CBICBICBICBICBICBI 

# CBI

CBI