# Patent

## Title
Methods for determining the hair follicle inductive properties of a composition

## Abstract

The present invention provides a method for determining hair inductive properties of a composition comprising injecting a composition comprising dissociated dermal cells and epidermal cells into skin of a mammal and determining whether at least one hair follicle forms.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
Not applicable
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT
Not applicable
BACKGROUND
It has long been recognized that the hair follicle regenerates over the life time of an individual and reproduces its lower half cyclically. New follicle formation can be induced experimentally by cellular manipulation. Studies of the cells which contribute to new follicle formation have been limited by the ability to assay these same cells for their hair follicle inductive, or trichogenic, properties. Various assays have been developed to determine hair follicle inductive properties including hanging drop cultures, granulation tissue beds, collagenous shells, kidney capsule cultures and an assay using an immunoincompetent mouse and silicon chamber (“the Lichti/Prouty assay”). Notably, the Lichti/Prouty assay is demanding in terms of cell number, time and animals required. The assay allows for only one sample per mouse, requires large numbers of cells per graft, requires a surgically intensive procedure and requires four weeks for results. Accordingly, there is a need in the art for new methods for assessing the hair inductive properties of human and other mammalian cells.
SUMMARY
In one embodiment, the present invention provides a method for determining hair inductive properties of a composition comprising injecting a composition comprising dissociated dermal cells and epidermal cells into skin of a mammal, wherein the dermal cells are from a first species and epidermal cells are from a second species, and wherein the first species is different from the second species; and determining whether at least one hair follicle forms, wherein the hair follicle is associated with at least one sebaceous gland, and wherein formation of at least one hair follicle indicates that the composition has hair inductive properties.
In another embodiment, the present invention provides a method for determining hair inductive properties of a composition comprising injecting a composition comprising dissociated dermal cells and epidermal cells into skin of a mammal, wherein the dermal cells are from a first species and epidermal cells are from a second species, and wherein the first species is different from the second species; and determining whether at least one hair follicle forms, wherein the hair follicle comprises an outer root sheath, an inner root sheath with companion layer, and dermal papilla, and wherein formation of at least one hair follicle indicates that the composition has hair inductive properties.
In a further embodiment, the present invention provides a method for determining hair inductive properties of a composition comprising injecting a composition comprising dissociated dermal cells and epidermal cells into skin of a mammal, wherein the dermal cells and epidermal cells are from human; and determining whether at least one hair follicle forms, wherein the hair follicle is associated with at least one sebaceous gland, and wherein formation of at least one hair follicle indicates that the composition has hair inductive properties.
In yet another embodiment, the present invention provides a method for determining hair inductive properties of a composition comprising injecting a composition comprising dissociated dermal cells and epidermal cells into skin of a mammal, wherein the dermal cells and epidermal cells are human; and determining whether at least one hair follicle forms, wherein the hair follicle comprises an outer root sheath, an inner root sheath with companion layer, and dermal papilla, and wherein formation of at least one hair follicle indicates that the composition has hair inductive properties.
Other aspects of the invention will become apparent by consideration of the detailed description and accompanying drawings.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 shows the complete structure of a hair follicle formed from in example 1, including a sebaceous gland (“SG”), bulge, inner root sheath (“IR”), outer root sheath (“OR”), dermal papilla (“DP”) and dermal sheath (“DS”).
FIG. 2 demonstrates that the newly formed hybrid follicles of the invention going through normal hair cycles.
FIG. 3 shows the histology of the hair growth cycle.
FIG. 4 shows new hair growth using the method of the invention using a composition of human dermal (1×106) and mouse neonatal epidermal cells (5×104 aggregates).
FIG. 5 shows new hair growth using method of the invention using a composition of human epidermal (0.5×106) and mouse neonatal dermal cells (2×106).
FIGS. 6A-6C show newly formed hybrid hair follicles stained for human centromeres (green) and mouse centromeres (red).



DETAILED DESCRIPTION
A novel method to determine the hair inductive properties of a composition comprising dissociated dermal and epidermal cells has been developed. The term “hair inductive properties” refers to the ability of cells to produce and/or induce new hair follicle formation. The term “trichogenic” may also be used to describe this property. The method comprises injecting a composition of cells into the skin of a mammal and determining whether at least one new hair follicle forms. A newly formed hair follicle may include the layered epithelial structure of a hair follicle, with a normal appearing hair shaft, internal root sheath with companion layer, dermal or follicular papilla and/or at least one sebaceous gland.
A newly formed hair follicle structure may have the ability to undergo cyclic growth demonstrating phases of anagen (the growing phase), catagen (the intermediate phase) and telogen (the shedding phase) similar to a naturally occurring hair follicle. A hair follicle formed during the method of the invention may cycle repeatedly through these phases or may only cycle a finite number of times or may only cycle once.
Suitably the dermal and epidermal cells are from different species to form a hybrid composition of cells. For example, the dermal cells may be from mouse and the epidermal cells may be from human. Alternatively, the dermal cells may be from human and the epidermal cells may be from mouse. The cells, whether dermal or epidermal, from mouse may be from a mouse of any age, e.g., a neonatal mouse or an adult mouse. Similarly, the cells from a human may be from a human of any age, including cells from neonatal foreskin. Cells from mouse and human are provided only as an example, other mammalian species may also be used in the methods of the invention. For example, dermal or epidermal cells may also be from rat or pig and combined with dermal or epidermal cells from mouse or human.
Alternatively, the composition of cells may be comprised of solely human cells, wherein the dermal and epidermal components are both from human. Though both components are from human, they need not be from the same human. Similarly, the dermal and epidermal components need not be from humans of the same or similar age. Accordingly, by way of example, the dermal cells may be from an adult human while the epidermal cells may be from neonatal foreskin.
Suitable cells are available commercially. For example, commercially available epidermal cells which may be used include, but are not limited to, adult or neonatoal, cryopreserved, human epidermal keratinocytes-APF from Cascade Biologics (Portland, Oreg.) or cryopreserved human outer root sheath cells from Cell Applications (San Diego, Calif.). Suitable commercially available culture media for epidermal cells includes, but is not limited to EPILIFE Medium from Cascade Biologics (Portland, Oreg.), defined keratinocyte serum-free medium (“DKSFM”) from Invitrogen (Carlsbad, Calif.), BRFF-EPM 2—a complete serum-free medium from Athena Environmental Sciences (Baltimore, Md.), outer root sheath growth medium kit from Cell Applications (San Diego, Calif.) or keratinoctye serum-free growth medium from Cell Applications (San Diego, Calif.).
Similarly, commercially available dermal cells which may be used include, but are not limited to cryopreserved hair follicle dermal papilla cells from Cell Applications (San Diego, Calif.) and WI-38 VA-13 subline 2RA from Istituto Ricerche Farmacologiche Mario Negri (Milan, Italy), Suitable commercially available culture media for dermal cells includes, but is not limited to hair follicle dermal papilla growth media from Cell Applications (San Diego, Calif.), MEMα or Fetal Bovine Serum, Qualified and Heat-Inactivated, both from Invitrogen (Carlsbad, Calif.). Alternatively, the dermal and/or the epidermal cells may be isolated from a subject and cultured prior to inclusion in the assay composition.
The composition may be composed of about one million to about four million cells total. Suitably, the ratio of dermal cells to epidermal cells in the range of about 500:1 to about 1:100, more suitably in the range of about 100:1 to about 1:20 and most suitably about 20:1 to about 1:5. For example, a suitable composition which may be used in the methods of the invention may contain 1×106 human dermal cells with 5×104 mouse neonatal epidermal aggregates. Alternatively, another suitable composition may contain 0.5×106 human epidermal cells with 2 or 2.5×106 mouse neonatal dermal cells.
Suitably, the composition of epidermal and dermal cells may be combined with a pharmacologically suitable carrier such as saline solution, phosphate buffered saline solution, Dulbecco's Phosphate Buffered Saline (“DPBS”), DMEM, D-MEM-F-12 or HYPOTHERMOSOL-FRS from BioLifeSolutions (Bothell, Wash.) or a preservation solution such as a solution including, but not limited to, distilled water or deionized water, mixed with potassium lactobionate, potassium phosphate, raffinose, adenosine, allopurinol, pentastarch prostaglandin E1, nitroglycerin, and/or N-acetylcysteine into the solution. Suitably, the preservation solution employed may be similar to standard organ and biological tissue preservation aqueous cold storage solutions such as HYPOTHERMOSOL-FRS from BioLifeSolutions (Bothell, Wash.).
The composition and the carrier may be combined to form a suspension suitable for injection. Each injection may comprise about 20 μl to about 100 μl of composition or suspension. More suitably, the injection may comprise about 40 μl to about 90 μl of composition or suspension. Most suitably, the injection may comprise about 60 μl to about 80 μl of composition or suspension. The injection may be performed with any suitable needle, syringe or other instrument. Suitably, a 25 gauge needle attached to a syringe loaded with the composition or suspension may be used. Alternatively, a hubless insulin syringe may also be used to inject the composition into skin of a mammal. The suspension may also be delivered by other suitable methods, such as spreading the composition or suspension over superficial cuts of the skin or pipetting the composition or suspension into an artificially created wound.
The composition may be injected into skin of any suitable mammal. Most suitable is an immunocompromised mammal such as a nu/nu mouse, or nu/nu rat. The injection may be placed into any layer of the skin such as the deep dermis, the papillary dermis, the reticular dermis or the hypodermis. It is most suitable that the injected composition be placed in the skin such that the cells of the composition remain in a confined space, regardless of the particular layer, such that the cells are limited in the degree to which they may spread. For example, injection into the deep dermis is most suitable because the physical structure of the deep dermis limits the degree to which cells may spread upon injection.
In a further embodiment, the method of the present invention may be used to determine not only the hair inductive properties of the composition of cells alone, but may also be used in determining the effect of other components on the cells' hair inductive properties. Suitably, additional components include, but are not limited to growth factors such as fibroblast growth factors, placental growth factor or vascular endothelial growth factor, cytokines, proteins involved in organogenesis signaling such as sonic hedgehog homolog (“SHH”), a sonic hedgehog homolog agonist, proteins in the Wnt signaling pathway, noggin, or lymphoid enhancement factor (“LEF-1”), molecular constructs, cells altered ex vivo, pharmaceuticals, scaffold materials such as collagen, laminin, MATRIGEL or any other extracellular matrix proteins.
Alternatively, other components such as cellular scaffolds or scaffolding materials may be added to the composition. This method may also be used to test other properties of the hair such as pigmentation by adding various amounts of melanocytes from different donors into the cell combination
As used in this specification and the appended claims, the singular forms “a,” “an,” and “the” include plural referents unless the content clearly dictates otherwise. It should also be noted that the term “or” is generally employed in its sense including “and/or” unless the content clearly dictates otherwise. All publications, patents and patent applications referenced in this specification are indicative of the level of ordinary skill in the art to which this invention pertains. All publications, patents and patent applications are herein expressly incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated by reference. In case of conflict between the present disclosure and the incorporated patents, publications and references, the present disclosure should control.
It also is specifically understood that any numerical range recited herein includes all values from the lower value to the upper value, i.e., all possible combinations of numerical values between the lowest value and the highest value enumerated are to be considered to be expressly stated in this application. For example, if a concentration range is stated as 1% to 50%, it is intended that values such as 2% to 40%, 10% to 30%, or 1% to 3%, etc., are expressly enumerated in this specification.
The following examples are provided to assist in a further understanding of the invention. The particular materials, methods and conditions employed are intended to be illustrative of the invention and are not limiting upon the scope of the invention.
EXAMPLE 1
Composition Injection into nu/nu mouse
Mature 6-14 week old nu/nu mice (Charles River Laboratories, Wilmington, Mass.) were anesthetized following USP IACUC approved protocol with ketamine (100 mg/kg) (Fort Dodge Animal Health, Iowa) or xylazine (10 mg/kg) (Phoenix Scientific Inc., St. Joseph, Mo.). A sedated animal was placed on a paper blanket in a tissue culture hood. The truncal surface of the animal was cleansed with 70% ethanol. Cellular compositions containing 1) dermal cells derived from C57/Black neonatal mouse (1-2 days old) (pregnant C57/Black female were purchased from Charles River Laboratories, Wilmington, Mass.) and neonatal human keratinocyes (Animal Product Free, Cascade Biologics, City State) cultured in EpiLife (Cascade Biologics, Portland, Oreg.) or BRFF-EPM2 (Athena Environmental Sciences Baltimore, Md.) or 2) hair follicle dermal papilla cells (Cell Applications, San Diego, Calif.) cultured in medium from Cell Applications (San Diego, Calif.) or WI-38 VA-13 subline 2RA cells from Istituto Ricerche Farmacologiche Mario Negri (Milan, Italy) cultured in MEMα (Invitrogen, Carlsbad, Calif.) with serum and epidermal cells derived from C57/Black neonatal mouse were suspended in DMEM/F-12. In this example, 1×106 human dermal cells were injected with 5×104 mouse neonatal epidermal aggregates or 0.5×106 human epidermal cells were injected with 2 or 2.5×106 mouse neonatal dermal cells. The cellular compositions were mixed with 60-80 ul DMEM/F-12 (Invitrogen, Carlsbad, Calif.). 60-80 μl of suspension was loaded into a syringe (Becton Dickinson, Franklin Lakes, N.J.) with a 25 gauge needle (Kendall Polypropylene Hub Hypodermic Needles, Mansfield, Mass., Catalog number 250313). An air bubble was allowed to form behind the sample and the base of the plunger to avoid introducing air bubbles into the cellular composition.
The cellular suspension was then slowly injected, up to 8-9 injections per mouse, into the deep dermis. The air bubble was also injected. The needle tip did not penetrate the whole dermis so that the cells of the composition can remain in a confined space in the dermis. Up to 8 injections were placed in each mouse. Each animal was tattooed at the site of implantation by piercing the skin peripheral to the injection site with a 25 gauge needle dipped in tattoo ink (242 Permanent Black Pigment, Aims, Hornell, N.Y.). Correspondingly, the left ear, right ear, both ears or the tail of the mouse was also tattooed or punched to distinguish individual mice in the same cage. The cage was then marked with the location of samples injected into each mouse, the experiment number and the date.
The injected cells were allowed to develop for 2-4 weeks. The cellular compositions comprised of mouse dermal cells and human epidermal cells required 14-20 days to form new hair follicles. The cellular compositions comprised of human dermal cells and mouse epidermal cells required 18-24 days to form hair follicles.
EXAMPLE 2
Injection Evaluation and Associated Histology
At the end of the incubation period the mice were sacrificed by CO2 narcosis. The full-thickness skin was collected using dissecting forceps, scissors and a scalpel with scalpel blade. The tattooed spots on each mouse were used to ensure harvest of all the implantations. Also included in dissection were both ears (and/or the tail) for orientation and to distinguish individual mice from the same experiment.
The full-thickness skin was laid flat on a piece of paper towel moisturized with DPBS (Invitrogen, Carlsbad, Calif.) with the mucosal side in contact with the paper towel. The skin and paper towel were placed in a 10 cm dish. The cover of the dish was then secured with tape. Each dish was labeled with the location of the samples injected in each mouse, the experiment number and the date. The dish containing the full thickness skin was transported on ice.
In the same dish the full-thickness skin was fixed in 10% formalin or 4% freshly made paraformaldehyde in DPBS for two hours at room temperature. The fixed skin was then rinsed three times with DPBS for 10 minutes each time.
Using a dissecting microscope, the mucosal side of the full-thickness skin was examined for hair follicles. A small pigmented “bump” was sometimes observed. The “bump” is formed by epithelial cysts and soft tissue at each implantation site. If necessary, the “bump” was torn apart to see the hair follicles hidden inside. Alternatively, the “bump” was turned to the reverse side to see the hair follicle formed at the bottom of the bump. Some follicles were twisted, curled or malformed due to lack of space for the shaft outgrowth. Some observed follicles were very fine with light pigment and other follicles were partially formed with the bulb but not mature shafts. All of these various forms of hair follicles were included in the count of hair follicles. The dissociated dermal and epidermal cells were found to form the complete structure of the pilosebaceous unit including at least one sebaceous gland, a bulge, outer root sheath, inner root sheath, dermal papilla and a hair shaft. Usually about 1 to 20 follicles were found assay using human dermal and mouse neonatal epidermal cells. Usually 1 to 150 follicles were found per hybrid patch assay using human epidermal and mouse neonatal dermal cells.
EXAMPLE 3
Distinguishing Human and Mouse Cells in Newly Formed Follicles
Staining with human or mouse-specific pan-centromere probes showed dynamic interaction between human and mouse cells. The human and mouse-specific pan-centromere probes were purchased from ID Labs Inc (human probe: CB1 579; mouse probe: CB1978M, 100 Collip Circle, Unit 117, London, ON Canada) As shown in FIGS. 3 and 4, the green represents human cells and the red represents mouse cells.
EXAMPLE 4
Study of Human Hair Follicle Cells in nu/nu Mice
The purpose of this experiment was to assess the ability of human hair follicle cells to grow in the immunocompromised mouse model. The starting materials for cell isolation were skin fragments or small pieces of scalp skin containing hair follicles from the Bosley hair transplant clinic in Philadelphia, Pa. The donors were males in their early 20s to late 60s. The tissues were rinsed in DPBS with antibiotics (1× penicillin/streptomycin/amphotericin (“PSA”) (Invitrogen, Carlsbad, Calif.) and weighed. The tissue was placed in plastic centrifuge tubes, containing D-MEM/F-12 plus 1× PSA immediately after surgery. One hundred and fifty (150) cellular composition mixtures of 1×106 human dermal cells with 0.5 to 4.0×106 human epidermal cells were prepared. The injections were performed as in Example 1. The number of hair follicles were counted whenever applicable and recorded as in Example 1.
One of the 150 composition mixtures using human cells produced a new hair follicle in the nu/nu mouse. Usually only follicular structures were seen on histology, but in one case with freshly isolated dermal and epidermal cells, 4 defined hair follicles with hair shafts were observed. The follicles were identified by histology as human-mouse hybrids.
Various features and advantages of the invention are set forth in the following

## Claims

1. A method for determining hair inductive properties of a composition comprising:
injecting a composition comprising dissociated dermal cells and epidermal cells into the deep dermis of an immunocompromised mouse, wherein the dermal cells are from a first species and epidermal cells are from a second species and wherein the first species is different from the second species; and
determining whether at least one hair follicle forms, wherein the hair follicle comprises an outer root sheath, an inner root sheath with companion layer, and dermal papilla; and wherein formation of at least one hair follicle indicates that the composition has hair inductive properties.

2. The method of claim 1, wherein the first species is mouse and the second species is human.

3. The method of claim 1, wherein the first species is human and the second species is mouse.

4. The method of claim 1, wherein from about one million to about five million cells are injected.

5. The method of claim 1, wherein the composition further comprises a carrier.

6. The method of claim 1, wherein the composition further comprises a component selected from the group consisting of growth factors, cytokines, proteins involved in organogenesis signaling, molecular constructs, cells altered ex vivo, pharmaceuticals, scaffold materials and combinations thereof.

7. A method for determining hair inductive properties of a composition comprising:
injecting a composition comprising dissociated dermal cells and epidermal cells into the deep dermis of an immunocompromised mouse, wherein the dermal cells and epidermal cells are human; and
determining whether at least one hair follicle forms, wherein the hair follicle comprises an outer root sheath, an inner root sheath with companion layer, and dermal papilla; and wherein formation of at least one hair follicle indicates that the composition has hair inductive properties.

8. The method of claim 7, wherein from about one to about five million cells are injected.

9. The method of claim 7, wherein the composition further comprises a carrier.

10. The method of claim 7, wherein the composition further comprises a component selected from the group consisting of growth factors, cytokines, proteins involved in organogenesis signaling, molecular constructs, cells altered ex vivo, pharmaceuticals, scaffold materials and combinations thereof.