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|Exhibit 23.1
CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM
Our report on our audits of the financial statements of Alfacell Corporation as of July 31, 2007 and
2006 and for each of the years in the three-year period ended July 31, 2007 and on the statements
of operations, changes in stockholders equity (deficiency) and cash flows for the period from
August 24, 1981 (date of inception) to July 31, 2007, included in this Annual Report on Form 10-K
for the year ended July 31, 2007, is dated October 4, 2007. Our report on Alfacell Corporations
internal control over financial reporting and managements assessment of Alfacell Corporations
internal control over financial reporting included in this Annual Report on Form 10-K for the year
ended July 31, 2007 is dated October 4, 2007. We consent to the incorporation by reference of our
reports in the Registration Statements on Form S-8 (SEC File Nos. 33-81308, 333-110074 and 333-112866)
and on Form S-3 (SEC File No. 333-112865 and 333-136678) previously filed by the Company with the
Securities and Exchange Commission pursuant to the Securities Act of 1933.
/s/ J.H. Cohn LLP
Roseland, New JerseyOctober 12, 2007

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