## FINAL REPORT

December 1996

Prepared For: FLORIDA POWER CORPORATION 3201 Thirty-Fourth Street South P. 

### 0. Box 14042 St. Petersburg, Florida 33733
Prepared By: CCI ENVIRONMENTAL SERVICES, INC. 5010 U.S. Highway 19, North Post Office Box 35 Palmetto, Florida 34220 

## s

~~~~~~~~~~~ 

## Bnc

CCI ENVIRONMENTALSERVICES, INC.

### TABLE OF 

### CONTENTS 

# Paae 1.0 INTRODUCTION 

# ........................................ 1
2.0 METHODOLOGY 

## ....................................... 4 

### 2.1 

### PilotStudy.. 

# ..................................... 4 

### 2.2 Full Scale 

### Study, 

## .................................. 17
3.0 RESULTS 

# ........................................... 28
4.0 DISCUSSION.. 

# ....................................... 54

REPORTS:ANCLSURV.STD FILE NO. 0125-008(041 

### CCI ENVIRONMENTAL SERVICES, 

### INC.

### LIST OF FIGURES
Figure 1. Figure 

### 2.

.r Figure 

### 3.
Figure

### 4.
Figure 

### 5.

Figure 

### 6.

Figure 

### 7.

Figure 

### 8.
Figure 

### 9. Sampling Station Locations, Anclote Plant Entrainment Study, Pasco County, Florida 

# .......................... 7 Plots of Corrected Survival Values for Each Replicate Sample at the Condenser Station for the Acartia 

### toma (A) and Amphipoda 

## (B)Species Groups Against Condenser Discharge Temperature 

# . . 39 Plots of Corrected Survival Values for Each Replicate Sample at the Condenser Station for the Chaetognatha (A) and Crab Larvae 

### (B)Species Groups Against Condenser Discharge Temperature 

# . . 40 Plots of Corrected Survival Values for Each Replicate Sample at the Condenser Station for the Caridean Shrimp (A) and lsopoda 

### (B) Species Groups Against Condenser Discharge Temperature 

# .. 41 Plots of Corrected Survival Values for Each Replicate Sample at the Condenser Station for the Pisces Juvenile (A) and Pisces Larvae 

### (B) Species Groups Against Condenser Discharge Temperature 

# ..................................... 42 Plots of Corrected Survival Values for Each Replicate Sample at the condenser Station for the Penaeid Shrimp (A) and Shrimp Larvae 

### (B) Species Groups Against Condenser Discharge Temperature 

# ..................................... 43 Plots of Corrected Survival Values for Each Replicate Sample at the POD Station for the Acartia tonsa (A) and Amphipoda 

### (B) Species Groups Against Condenser DischargeTemperature 

# ..... 44
Plots of Corrected Survival Values for Each Replicate Sample at the POD Station far the Chaetognatha (A) and Crab Larvae 

### (B) Species Groups Against Condenser DischargeTemperature 

# ..... 45 Plots of Corrected Survival Values for Each Replicate Sample at the POD Station for the Caridean Shrimp (A) and lsopoda 

### (B) Species Groups Against Condenser DischargeTemperature 

# ..... 46

REWRTSANCLSURV.ST0 FILENO. 0125-008W) CCI ENVIRONMENTALSERVICES, INC.LIST OF FIGURES (Continued)
Figure 

### IO. Plots of Corrected Survival Values for Each Replicate Sample at the POD Stationfor the Pisces Juvenile (A) and Pisces Larvae (B) Species Groups Against Condenser DischargeTemperature 

# ..... 47 Figure 11. Plots of Corrected Survival Values for Each Replicate Sample at the POD Stationfor the Penaeid Shrimp (A) and Shrimp Larvae 

### (B) Species Groups Against Condenser DischargeTemperature 

## ..... 48
LIST OF TABLES
Table 1. Summary of Abundance Data for the Dominant Species Collected During the Pilot Study, Anclote Entrainment Study, September 12,13and 14, 

### 1995. 

# .............................. 14 Table 

### 2. Average Survival of Species Groups by Replicate Sample, Sampling Event and Condenser DischargeTemperature, Anclote Entrainment Study. 

# ................................ 32 Table 

### 3. Grand Average, Corrected Grand Average, Standard Deviation, Frequency of Occurrence and Confidence Intervalfor Survivalof Each Species Group at Three Stations, Anclote Entrainment Study

# .......................................... 34 Table 4. Average Survival of Species Groups, after Correction for Control Station Mortality, by Replicate Sample, Sampling Event and Condenser DischargeTemperature, Anclote Entrainment Study. 

## .. 38 Table 

### 5. Results of T-test Comparisons of Corrected and Uncorrected Zooplankton Survival Means Between Sampling Stations and Condenser DischargeTemperatures for the Anclote Entrainment Study. Uncorrected Results Are in Parentheses. 

# ............ 50 Table 6. Resultsof T-test Comparisons of Corrected Average Survivalfor Each Species; at the Condenser Station Between Condenser Discharge Temperature Groups and Between the Condenser and Point of DischargeStations by Condenser DischargeTemperatures Groups, Anclote EntrainmentStudy. 

# .................... 52

REPORTSANCLSURV.STD 

## ... FILE NO. 0125-008(041 

## - 111 

## - CCI ENVIRONMENTAL SERVICES, INC.LIST 

### OF TERMS
Confidence lnterval

### - The rangie, above and below the average or mean, in which the true mean or average i3ctually occurs 

### with 

### 95% confidence limits. Control Station
- Intake canal station. Corrected Mortality

## - The mortality at the Condenser or Point of Discharge stations following adjustment for sampling related mortality. Calculated by subtracting the control station mortality, less its confidence interval, from 

### the observed mortality. 

### h e 

### - The average survival of a species group at treatment stations after correcting for mortality at the control station. Grand

### Avera% 

## - Average survival of a species group based upon data for all samples in which that species occurred. ate Samole 

### - For the purpose of this study, a replicate sample is one of the samples collected at i3 specific station for a sampling event. 

### Sgecies GrouQ

### - A group of irelated taxa which have been combined for the purpose of data analysis. Treatment Stations 

### - Condenser and Point of Discharge stations.

REWRTS:ANCLSURV.STO FILE NO. 0126-008(04) 

### - IV 

### - CCI ENVIRONMENTALSERVICES, INC.

~1.O INTRODUCTION The Florida Power Corporation Anclote Power Plant has been in operation near the mouth of the Anclote River iin Pasco County since October 

### 1974, when 

### Unit 1, the first 

### of two planned 

### 515 me!gawatt units, went on-line. As a means 

### of cooling the

- expected thermal discharge! plume from this unit, four dilution pumps were also installed in the intake canal for the plant. These dilution pumps were permitted by boththe U.S. Environmental Protection Agency and the Florida Department 

### of Air and Water Pollution Control (FDAWPC)totake cooler water from the intake side 

### of the plant and pump this water to the discharge canal. This water would then mix with the warmer condenser wateir dischargeand reduce the temperature 

### of the effluent to meetthermal discharge limits. The approval

### of this operating practicewas continued by the 1979 NPDES Permit for the operation 

### of Unit 

### 2 and has been included, to varying degrees, in all subsequent permit renewals.


### As a result of the 

### 1979 permit negotiations, FPC, The Florida Department 

### of Environmental Protection (FDEP, formerly FDAWPC) and USEPA reached an agreement for the construction 

### of twosidestream, "helper" cooling towers at Anclote Plant. The purpose of these towers was to augment cooling capability atthe plant, thus enabling FPC to meet new, stricter thermal discharge limits while avoiding

-1- CCI ENVIRONMENTALSERVICES, INC.frequent capacity deratings during the summer season as a result of high ambient temperatures in the intake.
As a condition of issuance of the initial operating permit for 

### Unit 1, FPC was required to performthe Anclote Post-Operational Ecological Monitoring Study (1 

### 976). This 

### study was performed bothto determine baseline ecological conditions inthe Anclote estuary and to determine any impacts from the initial years of plant operation. A major component of the overall study was a zooplankton populationstudy within the immediate area of the plant. Zooplankton nettowswere conductedthroughout 1976 and population densities were calculated for various species found both in the plant intake canal and in the Andote Anchorage (estuary). However, no sampling was performed in the plant discharges either to verify that actual entrainment of these populations was occurring 

### or to allow for mortality estimates to be developed. Therefore, 

### in the absence of actual entrainment mortality studies at the plant, the 1976 population estimates were used as a worst case assumption of entrainment losses through the plant systems. In fact, agency concern over the issue of entrainment, coupled with tlhe above worst case assumption, has been translated into flow minimizationconditions in the 1979 and all subsequent 

### NPDES Permit renewals. The flow minimizationcondlitions in these permits have included restrictions on both the dilution pump hours of operation and circulating water pump flow during the winter months. 

### In practical terms, these restrictions have led to a greater annual number of actual cooling 1:ower operating hours. Under certain conditions of intake temperature and plant loald, thermal discharge limits could be met by operating the


### -2- CCI ENVIRONMENTALSERVICES, INC.dilution pumps rather than cooliing towers. However, operation 

### of the dilution pumps under these conditions is seveirely limited by the current permit.


### In addition to retaining the above described flow limitations, the November 1994 NPDES permit renewal for the facility, issued by the Florida Department of Environmental Protection (FDEP, formerly FDER), contained a condition requiring FPC to perform a study to deteirmine 

### the actual mortality losses occurring due to zooplankton entrainmentthrough the dilution pumps. Florida Power Corporation first requested a continuance 

### of the!existing NPDES Permit to allow for certain conditions of concern to be discussed and possibly modified. After extensive negotiations with FDEP regarding these condiitions, the parties eventually agreed to perform an expanded zooplankton entrainment mortality study. The agreement was entered into by the parties with the condlition that the data would be used, if appropriate, to modifythe dilution pump restriictions inthe permit. The Plan of Study was designed to include an examination 

### of the actual mortality losses occurring through both the circulating water system (condensers) and the dilution pumps. If the study data demonstratedthat actual 

### total zooplankton losses through the cooling systems atthe plant would not be increased by using the dilution pumps for meeting thermal discharge limits under certaiin conditions, then this method of operation would be allowed by permit. The purpose of this report is to present the results of the completed 1

### 995 Anclote Zooplankton Entrainment Survival Study for that purpose.



### -3- CCI ENVIRONMENTALSERVICES, INC.2.0 METHODOLOGY CCI Environmental Services, Inc. developed the Zooplankton Entrainment Survival Study methodologywith direction and advice from FPC personnel. The Entrainment study consisted of two phases: a Pilot Study and the Full Scale Study. Both were developed based uponthe FDEP approved Plan of Study and the need to obtain data to answer specific questions [relativeto the study methodology itself (Pilot Study) and entrainment survival under various water temperatures (Full Scale Study). Provided below is the methodology used to conduct the study.
2.1 

### Pilot Studv The Pilot Study was conductedto address several questions outlined by FDEP as part of their review of the Plan of Study. The specific items to be addressed and resolved prior to implementation of tlhe Full Scale Study included:
a. Sampling methods, including depth and location; b. Methodsto miinimize sampling stress; c. Sampling intensity, 

### i.e., number of replicates to achieve statistical validlity; d. Diel effects on species composition, abundance and density in order to determine the proper sampling time of day; e. Study species selection; and f. Exact location of control station within the intake canal.


### In addition, the Pilot Study was usedto refine the field sampling and sample transfer procedures. Laboratory procedures for sample handling, tracking and processing
were also refined.

-4- CCI ENVIRONMENTALSERVICES, INC.Prior to initiation of the Pilot Study, a site visit of the Anclote Power Plant was conducted. The purpose of the site visit was to evaluate the sampling locations for accessibility and plankton net deployment. The field trailer was also inspected to determine initial sample transfer locations and improvements necessary for usage. Trailer dimensions and counter space was measuredfor analysis of sample processing station and water bath locations.
The zooplankton nets 

### (6) were custom made to handle the rigors of the field conditions, allow for rapid transfer of the samples and to minimize sampling related stress. The one-meter diameter nets had a 

### 400 micron mesh screen leading to the sample bucket. 

### A zippered mesh bag was attached to the cod end of the net and contained the sample bucket. Eight sample buckets were created using five-gallon plastic buckets equipped wit~h 

### two 500 micron mesh side panels. The buckets were placed 

### in the zippered bags and further fastened to the net with a stainless steel strap. The bucket design allowed the transfer of approximately 4 to 6 liters of sample (see attached photographs).
Based upon the trailer dimensions and anticipated number of sample bottles per replicate sample, water bathis were designed. The primary water bath was four feet wide, approximately four inches deep and twenty-nine feet long, consisting of a series of five smaller cells which interconnected. The bath was constructed of wood and double lined with plastic. Tlhis bath design was sufficient to contain bottles from 15 replicate samples.



### -5- CCI ENVIRONMENTALSERVICES, INC.Prior to sampling, quality assurance (QA) procedures and Chain of CustodylSample Tracking forms were developed. The Chain of Custody forms (reference Appendix A) stayed with the sample during collectionthrough final analysis and required logging of the samples in and out 

### of the water baths. Other information contained on the form included: Station, repkatenumber, sampling depth, date and time 

### of collection, length of sample tow, field personnel, laboratory recipient, initial sample processor, latent mortality sample processors and physical water quality data.
Prior to sample collection, the laboratory facilities were set up, This included filling the water baths, setting up the taxonomic sample processing stations and collection of the water to be used in the latent mortality bottles. Field mobilization included launching of boats, installing plankton buckets in nets and attachment of net tow ropes, floats and weights. A test run was conducted before the Pilot Study was performed. Thistest 

### run involvedthe collection and processing of a single replicate sample from each station.


### Field Sampling Samples were collected 

### in the Intake Canal, Condenser discharge and the Point of Discharge (POD) stations (Figure 1). The Intake Canal serves as a control and samples were generally taken as a stationary tow within 100feet ofthe condenser
or dilution pumps. One Intake Canal sample was a circular tow approximately 

### 700 feet long and upstream of the pumps. Initially, trawling within the discharge canal
was attempted to obtain a Condenser station sample. Sampling could 

### not be

-6- CCI ENVIRONMENTALSERVICES, INC.I 

### Thermal Sensors 

# l o 0 

# A Cooli ng POD 

### Towers

Outfall Canal

I\ 

### F1

### ume

# 47, C 

### Condenser. 

# + Di 

### scharge

P1

### ant Di

### 1ution



### Condenser
LEGEND C 

## - Condenser Station I 

### - Intake Canal/Control 

## .A Station I POD 

### - Point of 

### Discharge Station 0

Not 

### to 1-1 

### Scale



## u 

# m Laboratory

Trailers 

# /

Pump Di

### scharge

on Pumps 

### Bailey's 

## 3 B1uff -d 

### Road

Intake Canal

CCI ENVIRONMENTALSERVICES, INC.conducted from a stationary location in.this area due to 

### the turbulence and lack of directionalflow. All Condenser discharge samples were collected as stationary tows within the flume betweenthe condensersand cooling towers. The Point of Discharge

' 7 sampling location varied based upon cooling tower operation. When the cooling 

### ' towers were being used and the dilution pumps were off, the 

### POD samples were collected as moving tows downstream of the condenser discharge but upstreamof 

### the cooling tower dischargle. The remaining point of discharge samples were collected as stationary tows immediately upstreamof the thermal sensors with the coolingtowers off and dilution pumps on. Sufficienttime was allowed for the dilution pump/condenserdischarge to displace the cooling tower discharge prior to sampling.
Pilot Study sampling consisted of four distinct time and depth combination subsets 

### (Le., Day-Surface, Day-Bottom, Night-Surface and Night-Bottom). Sample collection occurred September 12-1

### 4, 1995. A total of 46 replicate samples (1

### 8 

### - Intake Canal, 17 

## - Condenser and 11 

## - Point of Discharge) were collected for the four time and depth combinations. An efFort was made to allow sufficient time between replicate sampling to minimize sample holding time prior to initial processing and to minimize latent mortality sampling overlap.
The zooplankton nets were generally deployed from stationary boats located 

### in the Intake Canal and at the 

### POD and from the cooling tower flume wall at the Condensers. A tow rope was attached to the bridle ring and the nets were slowly let backto ensure that air had been displaced and that the netwas "fishing" horizontally.



### -8- CCI ENVIRONMENTAL SERVICES, INC.

### For surface sample collection, the top of the net ring was deployed at 

### or within one foot of the surface. The length of the tow rope could be adjusted to raise or lower the net. 

### A second rope and weight were attached to the bridle ring to lower the net for mid (4-7

### ft. depth) or bolttom 

### (I0-1 

### 5 ft. depth) sampling. 

### A float was attached tothe top of the net ring, with a predeterminedamountof rope, to verify the net was sampling the appropriate depth (reference attached photographs). The float also served to keep the net mouth oriented vertically for proper sampling. The C station samples were all collected at the surface since the turbulence and velocity of flow through the condensers uniformly mixed the water.
The nets were deployed at varying time intervals based upon the flow velocity and to minimize the amount of debris collected. The tow times were also varied to determine the time necessary to obtain a sufficient number of organisms for latent mortality processing. Duriing the Pilot Study, tow times varied from five to thirty minutes. Following field collection, samples were left 

### inthe plankton net bucket, detached from the net and immediately transported to the field laboratory for processing. Accumulations of seagrass; blades were gently swished 

### inthe bucket and discarded prior to processing. Each bucket was individually numberedto allow it to be tracked through processing. Intake Canal samples were taken to the shoreline and immediately driven by truck to the trailer. One member of the field crew held the bucket to minimize agitationand spitling. Condenser and Point of Discharge samples



### -9- CCI ENVIRONMENTALSERVICES, INC.were taken directly by boat to the outfall canal shoreline immediately adjacent to the trailer. Sample transfer from the time the net was pulled until it was received by the laboratory was generally less then ten minutes. Sample Processinq Ail sampleswere received at the laboratory trailer by the Quality Assurance/ Quality Control Officer (QA/QC officer). The QA/QC officer signed for 

### the samples and 1 - I

recorded the time. Military time was used to avoid potential confusion on latent mortality sample processing. The QA/QC officer then relinquished the sample to one of the laboratory personnel for processing. Sample processing occurred separately for each replicate sample. Two types of sorting.were usedto identify the numberof live and dead individuals of each species. Smaller (microscopic) and more abundant species were analyzed within one or two 

### 25 cc draws after gently stirring the sample. The draws were counted on a gridded petri dish under a microscope. Larger (macroscopic) species were analyzed by sorting the entire sample. A magnifying ring equipped 

### with a light was used to view the macroscopic samples. All of the four time and depth combinations were processed 

### in this manner. Reference sp'ecimens for several species were preserved for subsequent, more detailed taxonomic classification.
One set of replicate sampleslwas processed for latent mortality observation. Latent mortality bottles were set up for the required number of species at each station. Intake Canal water was strlained through a 

### 355 micron mesh screen and used to fill



### - 10- CCI ENVIRONMENTALSERVICES, INC.each bottle. This mesh size was selected to ensure that no additional sample organisms’or predator species; were introduced, while still allowing phytoplankton for oxygen production and prey organisms to passthrough. Each bottle was labeled with
a unique number or alpha character combination which could be referenced to the date, time of sampling, station location, species introduced and number of individuals. Microscopic organisms were ,transferredto the latent mortality bottles by wide mouth pipette. Macroscopic organisms were transferred to latent bottles by eye dropper, spoon 

### or beaker depending upon their size and motility. -The initial emphasis of sample processing was the rapid transfer of live individuals of the selected species into latent mortality containers. The remaining number of live and dead individuals of each macroscopicspecies in each replicate was then determined by direct count of the entire sample. Up to 30 live individuals 

### of each dominant species per station per replicate were transferredto the latent mortality bottles. Most species were stored in 125 ml clear wide mouth bottles. Large individualswere stored in low numbers in 

### 500 ml bottles. Highly carnivorous 

### or cannibalistic 

### life stages, such as the megalopsand zoea larvae,
were stored individually in smaller jars to prevent predation. Upon reaching 

### 30 specimens or a complete count 

### of the sample, the number of individuals 

### in each latent mortality bottle was recorded. Organisms placed within the latent mortality bottles
were randomly selected on1 an opportunistic basis 

### (Le.,the first individuals located
were transferred).



### - 11 

### - CCI ENVIRONMENTALSERVICES, 

### INC.The latent mortality bottles were then placed in the water bath maintained at the appropriate temperature. Aeration was introduced as necessary into some fish

~


# 1

~ containers. Grow light(s) suspended above the water bath were used so that photosynthesis and natural oxygen production could occur for each sample bottle. Aquarium heaters were usedto heat the water 

### in each cell which was circulated by
a small bilge pump. Thermoimeters were placed 

### in each water bath cell. The water bath temperature was monitored constantly to maintain both at the canal average ambient water temperature. Hot water was occasionally added to raise the water temperature.
At approximately 

### 6, 12 and 

### 24 hour intervals after sample collection, each latent mortality bottle was inspectedto determine survival. Specimen bottles were swirled 

### infront of a light and a count of live specimens was recorded. For species notvisible without magnification, a miciroscope was utilized in an effort to count live specimens. Dead specimens were generally removed and preserved as they were found. The rapid nature of the initial sort of live organism precluded identification to the lowest practical taxon in some ca:;es. Therefore, upon completion of the latent mortality study, the preserved individuals were accurately identified. Thirty-six 

### (36) hours after sample collection, the remaining latent mortality bottles
were processed. The live organisms were counted within the bottles and 

### then preserved. The preserved specimens were viewed under a microscopeto allow for



### - 12- CCI ENVIRONMENTAL SERVICES, INC., .I identificationto the lowest practical taxon. Each species at each station for each replicate was processed separately.
The QA/QC officer's function, in addition to field sample acceptance, was maintenance of water bath 'temperature. The 

### QA/QC officer also ensured that the latent mortality analysis occurred atthe required time interval and that bottles were returnedto the proper bath location.


### A clipboard labeled 

### with the station and replicate identification number contained the Chain of Custody Sheet arid the sample processing sheets. This clipboard was suspended by a nail 

### on the water bath immediately adjacent tothe latent mortality bottlesfor that sample. The laterit mortality bottles for each sample were organized in individual nursery trays to prevent mixing with adjacent samples. 

## Results Table 1depicts a summary of the results of the Pilot Study. Information has only been provided for the most abundant species and those dominant species being entrained, exceptthat mostfish species were noted. The table presentsthe average number 

### of individualsfor each day-night and depth combination. The one condenser replicate collected 

### in the early evening 

### (6:20p.m.) has been separated due 

### to its unique speciescomposition. The POD samplestaken with the dilution pumps off (i.e., upstream fromthe cooling tower discharge) have also been separated from the other POD samples since they were almost exclusively influenced by the condenser discharge.



### - 13 

### - CCI ENVIRONMENTALSERVICES, INC.-14-

. CCI ENVIRONMENTAL SERVICES, INC.

### - 1.5

### - CCI ENVIRONMENTAL SERVICES, INC.The condenser station generally had 

### the highest abundancevalues, followed by the POD. Morespecies were geneirally represented at the Condenser and POD stations. These findings appear to be due to twofactors: 1)the condenser and, to 

### a lesser extent, POD station are uniformly mixed by the turbulence; and 

### 2) the velocity of flow, and thus sample concentration, is greatest atthe condenser station followed by the POD station. Flow at the lritake station was low with just the condenser pumps on and moderate with condenser and dilution pumps on. - The abundance and diversity of species was generally greater during the early evening to night hours. Most samples were taken on a rising to high tide and some tidal effects were observed. 

### For latent mortality, with only rare exception, those individuals which survived for 12 hours after sampling were still alive at 

### 36 hours after sampling.
The September 

### 21, 1995 reporttitled “Anclote Power Plant Zooplankton Entrainment Pilot Study,” prepared by 

### CCI Environmental Services, Inc. provides additional analysis of the results. This report also provides answers to the FDEP questions noted earlier. 

### The reader is referred to this report if more detailed information is desired.
Based uponth resultsof the Pilot Study, some methodologies and parameters of the Full Scale Study were further refined. The specific changes implemented are described 

### in detail under the Fiull Scale Study methodologysection below.



## - 16- CCI ENVIRONMENTALSERVICES, INC.

### 2.2 

### F uII Scale Studv The Full Scale Study was conducted following completion of the Pilot Study and analysis of its results. The 

### Full Scale Study's purpose was to determine the mortality caused by entrainment through the plant circulating water system and through the

4 dilution pumps. This information could then be usedto specify permit conditions for appropriate use of the dilutiam pumps and cooling towers to meet thermal discharge limits
- without increasing the current level of entrainment losses. Due to FPC's desire to increase use of dilution pumpsfor thermal control, the study was scheduled so that sampling would be performed generally within 

### the range of Intake and Condenser discharge temperatures at which dilution pumps would be usedto meetthermal limits. The higher temperatures witlhin this range were hypothesized to represent the upper tolerance limits of the study species and worst case mortality.
A total of 120 replicate samples were collected, 

### 40 at each of the three stations 

### (I, C and 

### POD). Twenty-five replicate samples were collected at each station from September 25-29, 1995and represent the upper end of the Intake Canal temperature range (28.4 

## - 31 

### .I

### "C)and Condenser discharge temperature range 

### (31.8 

## - 38.3"C) for the study. The next sampling event occurred October 9-1 1 

### , 1995 and involved the collection of 

### 30 samples; (IOreplicates per station) 

### within the temperature ranges of 27.8 

### - 28.2"C and 29.7 

## - 35.3"C for the Intake Canal and Condenser discharge, respectively. The final saimple collection occurred November 1 and 2, 1995 and included five replicates from each station at Intake Canal temperatures of 23.8 

## ­ 23.9"C and Condenser discharge temperatures of 28.8 

### - 30.5"C.



### - 17 - CCI ENVIRONMENTAL SERVICES, INC.Several modifications and refinements to the field and laboratory procedures were made as a result of evaluating the Pilot Study findings. 

### The following paragraphs describe primarily howthe methodology was revised from that of the Pilot Study and why the changes were made. Aspects of the Pilot Study methodology not specifically 

## i discussed are unchangedfrom that described in Section 

### 2.1 of this report.
Field Samplinq

- Field sampling was divided into eight sampling events. Each event consisted of the concurrent collection of five replicate samples from each station (1

### 5 samplestotal). Each event generally took eight or nine hours to complete.
The Pilot Study revealedthat the number of species and the number of individuals of each species increased after sunset (night samples). Based upon the observed species diversity and abundancevalues, the full scale study was primarily conducted at night. Sampling was iinitiated approximately one-half hour after sunset and continued 

### untilthe desired number of samples were obtained.
The September 

### 27, 

### 28 and 

### 29 events were exceptions 

### to the nighttime sampling schedule. These events were conducted at alternate times to reflect the effects of high Intake and discharge temperature and 

### in the hope of finding additional species. One event (September 

### 291) occurred from before sunrise (5:30 a.m.) until early afternoon, another event (September 

### 28) started mid-morning (1

### 0:30 a.m.) and proceeded 

### until evening (7:30 p.m.1, and the September 

### 27 event started late afternoon (3:30 p.m.1 and was completed

### by 10:45 p.m.



### - 18 - CCI ENVIRONMENTALSERVICES, INC. I 

## IThe sampling station locations remained basically unchangedfrom those used in the Pilot Study. All Condenser samples were collected as stationary tows within the cooling tower intake flume with the cooling tower pumps on to generate sufficient flow velocity within the flume. All Point of Discharge samples were collected as I stationary tows upstreamof the thermal sensors with the cooling towers off and the

~
, I , dilution pumps operating.. Intake canal samples were also collected as stationary tows, except for six replicates (two replicates each for the two October and one Novembersampling event). Movingtows were conducted during these events since flow was too lowto allow stationary tows. Operating conditions were constant throughout the study for each of the two treatments, as follows. Due to the necessity to have cooling towers on for the Condensersamples and off for the POD samples, and the complexity of startup and shutdown procedures, all five Condenser replicates were collected consecutively at the beginning of sampling with the cooling towers running and the dilution pumps off. Following collection of thefinal Condenser sample for that event, the cooling towers
were shut off and the dilution pumps turned on. Samples atthe POD station were 

### then collected after sufficient time (approximately 

### 20 minutes) was allowed to displace the cooling tower diischarge water. The Intakesamples, which represent the control, were uniformly distributed throughout the entire sampling event.
Plant operation was also manipulatedto represent the worst case conditions relative to zooplankton mortality. In this regard, the plant was operated at a peak load to



### - 19 

### - CCI ENVIRONMENTALSERVICES, INC.generate the highest change 

### in temperature 

### (AT) from the Intake Canal to the Condenser discharge. Peak load was maintained for as long as possible during each sample event to achievethese conditions.
Due to scheduled maintenance outages, only one unit was operational during the October and November sampling events. 

### To create flow proportional temperature conditions atthe POD during these events, only two of the four dilution pumps were operated. It was the partial use of dilution and condenser pumps during these events that necessitatedsome moviingtows in the Intake Canal toobtain a sample. High tide conditions seemedto reduce flow in the Intake Canalto the point where the moving tows were required to obtain a sufficient sample.
The Pilot Study results indicatedthat the type and number of organisms varied by sampling depth at the Control and the POD stations. This indicated that a depth composited sample was required. Therefore, sampling occurred at mid-depth 

### (4-7 feet deep) and surface for each replicate sample at these stations. To accomplish this, two netswere deployed siinultaneously fromthe boat. Both netswere towed concurrently for the same (durationbefore retrieval.
The duration ofthe stationary towswas standardizedfor each station and was based upon the ability to obtain a sufficient number of organisms for latent mortality analysis balanced against the stress caused by prolonged tows. The Condenser station was sampled for 20 minutes and the Intake Canal and Point of Discharge stations were sampledfor 

### :30minutes per replicate sample. The use of two nets and



### - 20 

### - CCI ENVIRONMENTAL SERVICES, INC.

### 30 minute tows allowed for 

### the collection of an adequate sample under the low flow conditions found atthe Intake and POD stations. Upon retrieval, the sample from one bucket was gently poured into the other sample bucket with the excess water being allowed to drain through the bucket mesh. The composite sample was then returned to the laboratory for processing 

### as a single sample. This method also i3ssisted in concentrating 

### the sample at these stations without increasing the stress associated with longer hold time 

### or sampling duration.
At the suggestion of Department staff, a hand rake was used to remove seagrass from the sample bucket, eliminating potential sample contamination caused by residual sunscreen or bug spray 

### on the samplers' hands.
Water quality data was also collected, 

### in situ, with a hydrolab during some of the sampling events. Data collected included temperature, pH, dissolved oxygen and salinity. Flow measurements atvarious depths were obtained, by Marsh-McBirney water current meter, during a few intake canal sampling events to determine where flow was greatest. The flow measurements revealed only minor variance in flow velocity betweenvarious depths inthe canal.
le Processing The Pilot Study revealed 

### the need to provide an increased number of laboratory personnel to minimize sample processing time. The goal was to remove all macroscopic latent mortality organisms and perform the counts within one hour of

- 21 

### - CCI ENVIRONMENTALSERVICES, INC.I i - sample receipt, thereby minimizing predation and other stresses. The species and life stages sorted microscopically did not appear to be adversely effected by longer hold times.
Initial sorting was accomplished by lab scientists specializing 

### in either micro or macroscopic processing. One 

### or more individuals were also dedicated to latent mortality sample processing. The hboratory coordinator was responsible for receiving samples from the QA/QC officer and assigning the sample for processing. The laboratory coordinator also assistedthe QA/QC officer to ensure that latent mortality bottles were reviewed at the designated time. A laboratory technician was responsible for labeling latent bottles, bottle washing and sample preservation.
The Pilot Study results indicated potential study species for the Full Scale Study. However, species selection for the Full Scale Study was based uponvarious criteria. First, the species must be abundantenoughto provide enough live individuals for use in the latent mortality study (approximately 

### 30 individuals/Replicate). Second, all study species were relatively abundant 

### in both the Control and 

### in the discharge samples, thus verifying the occurrence of through plant entrainment.
Based upon the observed abundance of organisms 

### in the Pilot Study, the following species were initially included 

### inthe 

### full scale study: 

### Acurfiu

### tomu,Menippemercemriu
zoea larvae, Penaeidea zoea larvae, Penaideajuveniles and Chaetognatha sp.



### - 22 

### - CCI ENVIRONMENTALSERVICES, INC. ISeveral species of amphipodls were present in sufficient number for study and two individual species 

### of amphipod were included.

I Dueto relatively low abundarices during the Study, Pisces species were evaluated as
analyzed individually 

### (Le.

### , one category for Pisces larvae and one for Pisces juveniles).

Sample processing also included the counting and analysis of any other species or species groups which were present in sufficient numbers. All abundant taxa were included in the study. Caridean shrimp and Isopods were included since they were consistently abundant. The crab larvae category included all species of crabs and both zoea and megalops lairval stages. The shrimp larvae category included both Penaeidea and Caridean shrimp larvae. All amphipod species were also combined as a single taxonomic group 

### for study purposes.
The Chain of Custody and Sample Processing Forms were revised based upon the Pilot Study results. Separate processing forms were preparedfor the microscopic and macroscopic processors tat improve 

### their efficiency. The initial target species were includedonthe preprinted form to expedite data recording. Copies of the forms are attached as Appendix A.



### - 23 

## - CCI ENVIRONMENTALSERVICES, INC.a Based on the Pilot Study, a latent mortality duration of 24 hours was determined to be appropriate. Generallyfive or fewer individuals of a species category were placed in each latent mortality bottle. Crab larvae were each placed 

### in their own jar to prevent predation and 15individuals were studied. Unlike the Pilot Study, apparently dead individuals were not removed from the latentjar until the 24-hour check. The 24-hour checks generally occurred under magnificationafter pouringthe sample in
a shallow dish. On several occasions, the 24-hour check revealed that individuals previously recorded as dead at 

### 6 or 12hours were actually alive.
The latent mortality water was collectedfromthe samplingstation and sieved through either a 125micron mesh sieve or 8 355 micron mesh sieve. The smaller sieve was used for the initial sampling events, but was replaced by a larger sieve so more phytoplankton would be present 

### in samples to provide oxygen. Nevertheless, the latent water was aerated by ian aquarium pump to minimize stress tothe organisms during the transfer process.
Several adjustmentswere made tothe water bath in order to maintainthe appropriate temperature. Submersible

### 200 or 

### 300 watt aquarium heaters were placed 

### in each cell of the water bath. 

### A garden hose was connectedfromthe sink tothe water bathto allow the direct addition of hiot water, if necessary. A bilge pump was used atthe low end of the system to primp water to the top of the system, which provided a continuous mixing and uniform temperature. The thermometers used in each cell
were calibrated against a certified laboratory thermometer to ensure their accuracy,



### - 24 

### - CCI ENVIRONMENTALSERVICES, INC.' I The air temperature in the trailer was controlled so it would not adversely affect water bath temperature.
The water bath was maintained within one degree of the average ambient water temperature of the intake canal for the previous seven days. The water bath target rangedfrom 29.9"C 

### - 30.3"C during the September event, 28.9"C for the October event and was 25.6"C for the November event. Microscopic analysis included four 

### 25 cc draws. Live and dead counts were performed on all four draws i3nd live organisms were collected to provide the required number for latent study. Additional draws were made if more latent organisms were needed.
On a few occasions, subsampling 

### of the macroscopic sample was conducted for species which were particularly abundant. After gently stirring the sample, a representative.portion of the sample was counted to obtain the initial mortality data. Quality assurance procedures were 

### also conducted during sample processing. Approximately one out of every 

### ten samples was designated as 

### a QA sample. This sample was coprocessed by two scientists or the results were checked by a scientist other than the sorter. The 

### CIA proceduresfollowed 

### the samplethrough its 

### 6, 

### 12 and 24 hour latent mortality checks ahd included confirmation of species identification and number.



### - 25 

### - CCI ENVIRONMENTALSERVICES, INC,Data Analvsis The data was entered into a computer from the sample processing sheets. As part of the QA procedures, 

### all datal entered was proofed by the QA/QC officer. The time, date, station, replicate number, species, initiallive and dead count and latent mortality j counts 

### at 

### 0, 6, 12 and 24 hours were entered. The temperature of the Condenser water discharge was recorded for the Condenser samples and the Condenser and

~ Point of Discharge temperatures were recorded for the POD samples. These temperatures are relativeto the time of sample collection and allow the data to be sorted by temperature. During the 24-hour latent mortality checks, a larger number of live individuals was occasionally observed than would be indicated by the previous observations. As long
as all live and dead individuals within the sample were accounted for, the data was revised to reflect the number of individuals found alive atthe 24-hour check.
The proofed and corrected1 data was transferred into the spread sheet program Quattro Pro for analysis. The initial analysis involvedthe calculation of initial, latent and overall survival for each species observed and each latent mortality bottle identified. Initial percent survival was determined by dividing the number of live organisms for a given species by the total number of live and dead organismsfor that species. Latent survival was determined by dividing the number 

### of individuals surviving at 24 hours for that bottle by the number initially placed in the bottle. Overall survival is obtained by multiplyingthe initialand latent percent survival values.



### - 26 - CCI ENVIRONMENTAL SERVICES, 

### INC.To accomplishfinal data analysis, several steps were required. First, the data needed to be composited to obtain a single survival value for each species. This was necessary since some categories, such as the crab larvae and shrimp larvae, were 1 representedby up tothirty latent mortalityjars and values which each factored into

~ the overall survivalvalue. 

### The data were also composited when several species were I combined at a higher taxonomic level due to low abundance for individual species.
The initial step was the finalization of the ten taxa/life stages to be analyzed. Based
on the composited raw data, the ten categories selected for further analysis were: 

### Acartia toma, crab larvae, shrimp larvae, Chaetognatha sp., Amphipods, Isopods,

Penaeid shrimp juveniie/adult, Caridean shrimp juvenile/adults, Pisces larvae and Pisces juvenile. These categories include those species, species groups and life stages which were consistenitlyabundant and 

### which representthe expected spectrum of sensitivity to heat stress,and turbulence. Species codes were assigned to each category to assist 

### in data reduction.
The data was then reduced suchthat each species group was represented by a single value. This manipulation provided a single initial, latent and overall survival value for the ten species groupsfor reach replicate.
Meansurvivalvalues were calculatedfor each species, replicate sample and sampling event. The mean and standarddeviation were calculated for each station by species and overall for all species. The 95% confidence interval aroundthe mean was also calculated to determine the potential range of the actual mean.



### - 27 

### - CCI ENVIRONMENTAL SERVICES, INC.In 

### an effort to determine the effect of Condenser discharge temperature on survival, additional analyses were performed. The overall survival value for each species by replicate was adjusted for Control mortality. The observed mortality for a given species at the control station (average of all 40 replicates) was reduced by the observed confidence interval1 for that species. This value represents 

### the lowest 

# 1 .' 

### I possible mortality (at 

### 95% confidence) due to natural means and sampling stress. This value was 

### then added 'to the observed survival values for that species for the Condenser and Point of Dischiarge samples. This resulted 

### in survival values at these stations which mostaccurately reflect the effects of entrainment. The survival results
wwethen plotted by Condenserdischarge temperature for each species. Regression analyses were performed 

### for these data plots to determine if any statistical trends 

### in survival occur based upontlie Cdndenser discharge temperature.
T-tests were also performed between the various stations and average Condenser discharge temperatures to determine if the average survival values observed were significantly different.



### 3.0 RESULTS 

### A total of eight sampling events, each consisting of the collection and processing of five samples from each station, were conducted. The ambient temperature of 

### the Intake Canal varied from 

### 23.8 to 31.1"C during sample collection. Five sampling events occurred at the uplper end of the intake canal range (28.4 

## - 31 .I "C), two



### - 28 

### - CCI ENVIRONMENTALSERVICES, INC.

### 1 events atthe middle of the range (27.8 

## - 28.2"C) and one event atthe low end of the range (23.8
- 23.9"C).
The Condenser discharge temperature varied from 28.9 to 38.3"C during the study. The Condenser discharge tempbrature was affected both by the Intake Canal temperature and the plant load. The higher the load, the greater the change 

### in temperature (AT) from the Intake Canalambient temperature. The highest Condenser temperatures were reached during the September events, with the lowest temperature recorded for the November event. The AT varied from 4.0 to 7.0"C throughout the study, depending onthe above factors.
The species composition anld diversity varied somewhat by the time of day and by the tide. As observedfor the Pilot Study, the diversity of species was low during the middle part ofthe day but increaskd in the early morning and early evening. Species such as Chaetognatha sp. arid Penaeid shrimp were generally absent fromthese midday samples. Generally speaking, there were no apparent patterns in the occurrence ofthevarious taxa duringthe 

### full scale study. An exceptiontothis was the absence of Chaetognatha sp. atthe Conddnser station 

### during bothOctober events. Duringthe September 

### 29 sampling, a wave bf fish eggs appeared to have been brought in near the end 

### of the event 

### by a hightide. Otherwise, fish eggs were generally absent.
The relative abundance of speciles varied some over the course of the study based uponthe time of day and date of sampling. As noted in the Pilot Study, organism abundance was 

### low during the middleof the day. 

### Acartia

### tuma abundance appeared



### - 29 

### - CCI ENVIRONMENTAL SERVICES, INC.I highest in 

### the early evening hours and Chaetognatha sp. abundance appeared to increase throughout the night. Isopod abundanceappeared greatest in October as did that of the Amphipods and Pisces larvae. Caridean shrimp juveniles increased in abundance 

### in October and November. 

### Acartia

### tonsa was somewhat more abundant in November and amphipod abundance remained 

### high.
An attempt was madeto comipare the results of this study with those of the Anclote Post-operational Ecologkal Monitoring Program (1976). 

### Acartiatonsa was by far the dominant species observed throughout the year in the 1976 study and for the duration of this study. A total of 

### 27 species of ichthyofauna and commercially important crustacea larvae anid post larvae observed throughout theyear 

### in the Intake Canal during the 1976study were also observed during this study. Almost all of the most abundant species in the 1976 survey were recorded during this survey. Therefore, the results of this study appear representative of the species composition previously identified 

### inthe 11976 study.
A taxonomic list of all species observed during the study is presented 

### in Appendix B. Appendix C contains a list of the ten species groups used, the species code and a taxonomic list of the species included 

### in the analysis for each species group. Of the species contained 

### in the ten taxonomic groups, 12 were ranked 

### in the top five dominant species during various months in 1976.
Attached as Appendix D are the observed survival values for each station grouped by species. As discussed earlier, the observed survival at the Condenser and Point of



### - 30 

### - CCI ENVIRONMENTALSERVICES, INC.Discharge stations were increased by Control mortality for that species based upon the Intake Canaldata. The adjustment to the survival of each species is depicted in the "Sample Mortality" box 

### in the Intake Canal (Control) station tables 

### in Appendix D. The survival values adjusted for Control mortality are depicted under the
I 

## ! "Corrected Survival" column ;and are represented 

### in the Grand Average calculationfor I 1 the Condenser and POD stations.

~ The September data can be! divided into subsets based upon the average AT and Condenser dischargetemperature for the sampling event. The Intake Canal average temperature was 30.0"C for all five events. However, the average Condenser discharge temperature and AT were 37.6"C and 

### 7.1 "C for the September 25-27 events, whereas the Condenser temperature and AT were 35.0"C and 5.6"C for the September 28 and 

### 29 events. The Condenser dischargetemperatureswere similar for the September 28 and 29 events and the October events, butthe AT was highest in October (7.1 "C). The October and September 

### 28 and 

### 29 results were comparable. The September 25-27 mortalities were noticeably different from the October and September 

### 28 and 29 events. Thb mortalitieswere higher and/or the range of results
was wider for all speciesgrctups, except Isopods, during the September 25-27 event.
Provided 

### in Table 2 is a. summary of the uncorrected average survival for each replicate and sampling event 

### by station. The results have been grouped by the average Condenser dischargeterhperature at the Condenser station for the sampling event. Temperature Group 1 (TI) has average Condenser discharge temperatures at



### - 31
- CCI ENVIRONMENTALSERVICES, INC.

- I0

U C 6 0 .­ 4.4 m 4.4 m L v) C 

### 7 0 W 5 c Q .- 

## E
c Q 'El

INC.the upper end of the range (37.1 

### - 38.1 "C) and includes the September 25-27 sampling events. Temperature Group 2 (T2) has average Condenser discharge temperatures of 34.5 

### - 35.5"'Cand includes the September 

### 28 and 

### 29 and October

I 

### 9-1 

### 1 sampling events. The Novedber sampling event makes upTemperature Group
I 

# i' 3 (T3) and has an average Condenser discharge temperature 

### of 29.6"C. 

## I 0 

### As expected, survival was lowest at the Condenser station averaging 55.5% (before correcting) for all species groups and sampling events. Survival at the Point of Discharge averaged 9.5% higiher than the Condenser station overall at 66.0% (before correcting). Survival observed during the October and November events was generally higher than the observed September survival values.
Based uponthe overall samplling results at the Control station, mortality due to natural
causes and sampling stress was 31.8% which could be decreased by the confidence interval 

### of 3.7% to 

### 28.1 %. Correctingthe overall means for control station mortality results in overall survival !values of 83.6 and 93.1 for the Condenser and POD stations, respectively.

1 I e. ! 

### Table 

### 3 summarizes the observed results 

### by station for each species. Control mortality, as determined from 

### the Intake Canal station, averaged 32.8% overall and
was decreased by the confidence interval to average 

### 22.2. This adjusted mortality
was quite variable between species, ranging from a low of 

### 6.5 percent for Penaeid shrimp to a high of 42.7 and 37.2 percent for Chaetognatha sp. and

### Acam'a tonsa, respectively. The mortality values used to correct the treatment survival values for



### - 33 

## - CCI 

### ENVIRONMENTAL SERVICES, INC.I- 0 Q) 0 C? I- C? t F t C? r4 

## -a z n 

# 23

0 

## - .- C al > a 

### .­ 8 0 i?

L

CCI ENVIRONMENTAL SERL'ICES, INC.E I I I : I :

? ' c! b c

(v (v


# 2 m

i'iu

* m m *eo cchl

Q, m



### - 

# 5 Q 

### .­ 2 v)



### CCI ENVIRONMENTALSERVIC :ES, INC.each species representthe lowest mortality due to sampling with 95% confidence. Correcting the observed survival occasionally resulted 

### in values in excess of 

# loo%, however, 

### 100%was used as the upper 

### limit for survival.
As expected, survival values varied by species for each replicate sample. High average survival values 

### of 

### 91.5, 

### 139.5, 84.6 and 

### 79.4 were recorded atthe Condenser station for

### Acmu 

### fonsa,Crab larvae, Shrimp larvae and Isopods, respectively. Lower average survivals of 48.5% and 

### 27.2% were observed 

### for the Amphipods and Pisces larvae, respectively, atthe Condenser. The remainingspecies groups had moderate survival values 

### from 

### 63.8 to 

### 69.9 percent.
Survival observed at the Point 

### of Discharge station generally paralleled that of the Condenser station. Again, Crab larvae,

### Acm'a, Shrimp larvae and Isopods had the highest observed survival, but no species exhibited low survival. The observed survivals of several species groups 

### (Acama toma, Crab larvae, Chaetognatha 

### sp., Isopods, Piscesjuveniles, Penaeid shrimp and Shrimp larvae) were close enough at both stations such that the corrected grand averages fell 

### within each other's confidence interval. That is, the observed survival of these species groups at the Condenser and Point

### of Discharge stations were notsignificantly different from each other, thereby falling within the 95% confidence interval. Only Pisces larvae, Amphipods and Caridean shrimp exhibited substantially greater survival, 

### 35.0, 24.4 and 

### 16.7 percent, respectively, atthe Point of Dischargestation.



### - 36 - CCI ENVIRONMENTALSERVICES, INC.;

.. . The standard deviationand confidlence interval values 

### in Table 

### 3 provide information
as to the variability of the observed survival values. The 

### 95% Confidence Interval .isthe range 

### within whichthe true survival mean is expected tofall 

### 95% of the time. The confidence intervals are acitually quite small, ranging from 

### 4.7 to 17.3 at the Condenser and 

### 5.0 to 

### 13.4 atthe Point of Discharge for each species.
Table 4 provides a summary (of the average survival for each replicate at the Condenser and

### POD stations after correction for Control station mortality. The table ispresented in the same format as Table 

### 2. It is interestingto notethatthe overall survival atthe treatment stations is lessthan the overall survival expectedfrom Table 

### 2 

### (Le., 83.6% at Condenser and 

### 93.1% at POD). This is the result of two factors used in determining the corrected survival. First, the mortality values used for correction were calculated by species, which resulted 

### in larger confidence intervals 

### (10.7%vs. 

### 3.7%) and lower mortality values 

### (22.2% average vs. 

### 28.1

### %). Limiting corrected survival to 

### 100%also was a factor since the entire mortality value could not be used in several cases. The corrected overall survival value and confidence intervalfor the Condenser and P’ointOf Discharge stations are 

### 69.4% and 4.3%, and 

### 78.8% and 

### 3.9%, respectively. Therefore, the overall Control station mortality adjustment to the Condenser and POD stations after correction was 

### 13.9% and 

### 13.8%, respectively.
Providedas Figures 

### 2 through I1are plots of the corrected survival values for each replicate sample plotted against the Condenser discharge temperature for each

- 

### 37 ­

~ CCI ENVIRONMENTALSERVICES, INC.INC.P

i 

# In

CondenseirStatidn

0%25.0 30.0 35.0 40.0 CondenserTemperature ("C) 

## I I Figure 2. Plots of correcteid survival values for each replicate sample atthe Condenser Statim for the Acartia 

### tonsa (A) and Amphipoda (B) species groups against condenser discharge temperature.



### -,39 - CCI ENVIRONMENTALSERVICES, INC.I

F. e ."

.. 1ooo? 

# A
4, 80%

0 0 6%

40%

20% Condenser Station

0% 1 , , , * 1 , t 1 , 1 25.0 30.0 35.0 40.0 CondenserTemperature ("C)

100%

e 80%

60%

40% CondenserStation 0 20%

I , , . , I , , , , I

0%25.0 3b.O 35.0 40.0 CoddenserTemperature 

### ("C) Figure 

### 3. Plots of corrected survival values 

### for each replicate sample at the Condenser Station for' the Chaetognatha 

### (A) and Crab Larvae (9) species groups against condenser discharge temperature.

- 40 

### - CCI ENVIRONMENTAL SERVICES, INC.BOX (d .- >

U Q) -c. 0 2 40%

0%25 0

100% ­ 

# -B
80% ­ 

### - .- 

## 9 L =I 60% ­ 

### cn U 

# - Q) 0 L 

### E 40% ­ 

## 6 

# ...

30.0 35.0 400 CondenserTemperature(“C)

*a *em a


# . a t


# . e a4 .a

a 8 a
CondenserStatibn

I I . ’ ~ I ~ ~ * * ‘ 0%25.0 30.0 35.0 400 CondenserTemperature (“C)
Condenser Station far the Caridean Shrimp (A) and lsopoda (B)

- 41 - CCI ENVIRONMENTALSERVICES, INC.species groups again$t condenser discharge temperature.



### - 42 - CCI ENVIRONMENTALSERVICES, INC.100%

# 1- -0 

# . 0.

60% . e

20% Penaeidshrimp

0%25.0 30.0 35.0 40.0

100%

# 1

80%

60%

40% w,&

0% CondenserTemperature ("C)



# . 

# .

. .

0 . CondenserStation 

# L A Shrimp liarvae

L , , , 

### , , , , , 

### , 25.0 30.0 35.0 40.0 CoqdenserTemperature("C) Figure 6. Plots of corrected survival values for each replicate sample at the Condenser Stationfor the Penaeid Shrimp (A) and Shrimp Larvae 

### (9) species groups against condenser discharge temperature.

- 

### 43 - CCI ENVIRONMENTALSERVICES, INC.

# i

60%

40% 

## I

# I

DDh25.0 30.0 35.0 40.0 CondenserTemperature ("C)

- 100% 

# B

- 80%
e

- 60%

- 40% 

# -1i:I PODStation


# .

0% : * I ~ ~ ' ' ' * ' ~ ~ I

25.0 30.0 35.0 40.0 CorldenserTemperature ("C)

: Figure 

### 7. Plots of corrected survival values for each replicate sample at the POD Station for the Acartia tonsa (A) and Amphipoda (B) species groups against condenser discharge temperature.

- 44 - CCI ENVIRONMENTAL SERVICES, INC.Chaetogniatha

0%25.0 30,O 35.0 40.0 CondenserTemperature ("C)

100%

80% j

B; 

## r­ e.

- ti; 0 0 8 8 60%

40%

209c

0% Crab Lawae

1 .II""'"' 25.0 30.0 35.0 40.0 CondenserTemperature ("C)
POD Station for the Chaetognatha (A) and Crab Larvae 

### (B)species groups against condenser discharge temperature.



### - 45 - 

### CCI ENVIRONMENTALSERVICES, INC.100%


# . 8

# . e 80% e

60%
P 

### * e e 40%

0
205( PODStation 

# . Caridean Shrimp 

# 7

- 0% 25.0 ‘30.0 35.0 40.0 CondenserTemperature (“C)

100% e. 0 

# . *.

re a 8099

60% 

# .

40%

c 2096 

# . lsopoda

0%

25.0 30.0 35.0 40.0 CondenserTemperature 

### (“C) Figure 9. Plots of correcteld survival values for each replicate sample at the 

### POD Station for the Caridean Shrimp (A} and lsopoda species 

### (B) groups against condenser discharge temperature.

- 46 - CCI ENVIRONMENTAL SERVICES, INC.0 0 0. 0-0 0 - 

# .

0 0 PiscesJuvenile

P a 0 I)- :.
0 0

0

.I

0

' I n ' ' ' a " ' I . O%L------S 25.0 30.0 35.0 40.0 

### CondenserTemperature 

### ("C)

100%

# 1 4D 0 eo 0 0 0 

# B
0 80% 0
m W 0
0 60% 0



# . 40% 0

20% 0.. 0.0 0 PiscesLarvae

0%

25.0 3Q.O 35.0 40.0 

### CondenserTemperature 

### ("C)

, 

### Figure 

### IO. Plots of corrected survival values for each replicate sample at the POD Staition for 

### the 

### Pisces Juvenile (A) 

### and Pisces Larvae (B) 

### species {groupsagainst condenser discharge temperature.

- 

### 47 - 

### CCI ENVIRONMENTAL SERVICES, INC.1.'

i a 0 

### i - 80% 0 %. 

## I 'lb a 0

60% 

# I

c

m a

20%

# 1 POD Station

# I

Penaeid Shrimp
0%25.0 30.0 35.0 40.0 

# L~''''''''"L CondenserTemperature("C)

60% a

40%

# i 1

PODStation Shrimp Larvae

vm 25.0 30,O 35.0 40.0 CondenserTemperature ("C) Figure 11. Plots of corrected survival values for each replicate sample at the POD Staltion for the Penaeid Shrimp (A) and Shrimp Larvae (B) species (groupsagainst condenser discharge temperature.

- 48 - CCI ENVIRONMENTALSERVICES, INC.species and treatment station. Since some replicate samples were collected at the
same temperature and the species had the same survival value, a dot on the figure may represent more than one data point.
The Condenser and POD stations have the same general pattern of survival based upontemperature. 

### No clear trends can be observed on the plots, except that survival generally declines and becomes; more variable as the temperature increases. The Amphipod results at the Condenser station are an exception and show a clearer temperature related decline 

### in survivdl. Regression analyses were performed on the survival versus temperature data as reflected in the plots and the results are presented in Appendix E. No statistically significant trends were observed and the correlations were generally weak. However, it is possible that clearer mortality trends would emerge at higher Condenser discharge temperatures.
Table 

### 5 contains the results of T-test comparisonsfor uncorrected survival for 

### the entire study as 

### well as by temperature group. Information on corrected survival comparisons between

### the POD (andCondenser stations is also included. The results 

### inthis table are presentedto depict where observed survival is significantly different based upon the comparison of different temperature groups and stations. The uncorrected overall survival at tlhe Condenser is significantly different than the Control and POD station, whereasthe POD and Control stations are notsignificantly different. The temperature group comparisons show significantly lower survival at the

- 49 

### - CCI ENVIRONMENTALSERVICES, INC. 1I v)

0 W

W 1

W

W VI 

# - W 

## - 0) 1 1 1 1 Q : : I : E 

# E m m tn tn W w w m m 

### .- 

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### E 

### E Lo 0 c (u 0 0 w w tn v) U U I C C I 0 0 Q Q In v) 

### E 2 L L 0 0 0 0 I I
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### u 0 0 0 c- Lo ad Lo l7 m i I 

# - I Lo I; e i I m m I 'c 0 \c 0 v) v) 2 L 3 w + 

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### E 

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### P P m J= m I 0 e- I rn .- U $. $.

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### 5 C aJ L m P C 

### .­ 

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### b 0 C 3 

### * CCI ENVIRONMENTALSERVICES, INC.Condenser station for the two highest discharge temperatures 

### (Tl and T2), but no difference atT3 when comparedto the POD station. Temperature group comparisons 

### for the Condenser station reveal significant differences in survival between each temperature group pair. The 

### TI group survival at the POD station was significantly lower than the T2 and T3 groups, whereas the T2 and T3 groups were similar.
To determine which species groups were responsible for the observed significant differences 

### in survival between stations and/or temperature groups (as depicted 

### in Table 

### 5), additionalT-test analyses were performed using the corrected survival data. The results of theseeanalyses are reflected in Table 

### 6. Except for the Isopods and Pisces juveniles, all species groups exhibited some significant differences between temperature groups at the Condenser station. Seven species groups demonstraled significantly lower survival inthe 

### TI group when compared to the T2 and T3 groups. Only four species groups (Amphipoda, Caridean shrimp, Pisces larvae and Penaeid shrimp) had significantly different survival when the 

### T2 and T3 groups were analyzed.
Review of the comparisons of corrected grand average survival for each species group between the Condenser and POD stations indicates that three species (Amphipoda, Caridean shrimp and Pisces larvae) have significantly lower survival. 

### The corrected overall survival of the POD station was notably higher 

### for Chaetognatha, Isopoda, Pisces juvenile and Penaeid shrimp, but these differences
were not statistically significant.

- 51 - 

### CCI ENVIRONMENTAL SERVICES, INC.I v) v) v) v) 

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### - - ~ CCI ENVIRONMENTALSERVICES, INC.

- 53 - iThe Condenserand POD stations were also compared by temperature group for each species group. Only three species, 

### Acartia tonsa, Crab larvae and Penaeid shrimp,
were not significantly different for any temperature group. Six species had significantly higher survival at the POD station within temperature group 

### 1. Only Amphipods and Caridean shrimp had Significantly higher survival at the POD for the 

### T2 group, whereas shrimp larvae were significantly higher at the Condenser. Overall, the T3 group was not significantly different between the two statipns, however, Caridean shrimp and Pisces jiuveniles had significantly higher survival at the Condenserstation. Four species (Chaetognatha, Isopoda, Piscesjuvenile and Shrimp larvae) had one significant difference 

### in a temperature group comparison but their Grand Average was not significantly different.

4.0 

### DISCUSSION The sample collection and processing procedures combined with natural mortality resulted 

### in an overall average mortality at the control station of 31.8% (Table 

### 2). Unfortunately, natural mortality cannot be separated for sampling related mortality to

E. .. evaluate the sampling and sorting procedures. Review of the sampling procedures indicates that they were standardized appropriately to reduce sampling stress as much as possible for the purploses of this study. Importantly, the collection and sorting procedures were standiardized which allows unbiased comparison of results betweenstations and sampling events.

- 

### 54- CCI ENVIRONMENTALSERVICES, INC.3

$: The corrected overall mortality averaged 30.6% for all events at the Condenser station (Table 4) with a confidence interval of 4.3%. The overall mortality was significantly different (higher) than that of the Control station and Point of Discharge (Table 

### 5). There is a trend of higher mortality at higher Condenser discharge temperatures based uponthe temperature groups investigated in this study.
When comparingspecies group mortality at the Condenser (Table 

### 6), differences are observed. Generally, mortality became more variable and higher as the temperature increased. Only Isopods, Piscesjiuvenile and Pisces larvae did not display significantly lower survival in the TI group (37.6"C average Condenser discharge temperature) when comparedto T2. Isopods ;and Piscesjuveniles were also not significantly lower for the TI to T3 comparison vvhereas the Pisces larvae were significantly lower. Mortality was also significantly higher for the T2 group when compared to the T3 group, but only four species groups were significantly different. Seven species groups were significantly different for the T I

### -T2 and T I-T3 comparisons, apparently indicating more uniform stress betweenspecies groups at higher Condenser discharge temperatures.
The Isopods and Piscesjuveniles data indicates variable survival at all temperatures and the mortality for these speciies may be more directly relatedto the physical stress of entrainment and less associated with the increase in temperature. The results appear to indicatethat the Condenser discharge temperature is more directly related

- 

### 55 - CCI ENVIRONMENTALSERVICES, INC.*. to mortality than isthe AT since the October and September 28 and 29 results are comparable even thoughthe AT varies by 1.4"C.
Entrainmentmortality atthe Point of Dischargewas also low. The corrected overall mortality averaged 21.2% with a confidence interval of 

### 3.9% (Table 

### 4). The overall mortality was significantly lowerthan the Condenser station (Table 

### 5), probably due to the 

### high survival of the dilution pumps upstream contribution. When comparing

- species group mortality for the POD station, the trends paralleled those of the Condenser station.
Entrainment mortality atthe 

### POD station was lowestfor the 

### T2 and T3 temperature groups (Table

### 4). Mortalityatthlisrange of Intake Canal 

### (23.8 

### - 30.2"C) and average Condenser discharge 

### (29.6 

### - 35.0"C) temperature, with the average AT of 6.2"C, is minimal. Overall survival was nlearlyidentical for the 

### T2 and 

### T3 data, 

### but individual species groups exhibit more variability 

### in mortality atthe higher temperature.


### As mentioned earlier, the avelrage overall survival at the Point of Discharge was significantly higher thanthe Condenser station survival. The differences in mortality
were primarily attributable to the 

### TI and 

### T2 temperature group sampling events (Table 

### 5). The overall survival resultsfor the

### T3 sampling event were nearly identical 

### for these stations (Table

### 4). The largest differences 

### in survival were noted during the TIand 

### T2 events at 15.5 and 

### 7.6 percent, respectively.

- 

### 56 - 

### CCIENVIRONMENTAL SERVICES, INC.The species group mortality trends betweenthese stations, relative to temperature,
are similar. This is not unexpected since the Condenser discharge comprises a portion of the water sampled at the Point of Discharge station. Three species groups, 

### Acurfiu tomu, Crab larvae and Shrimp larvae, had nearly identical overall mortality (Table 

### 3)and mortality trends. The Chaetognatha sp., Isopods, Piscesjuveniles and Penaeid shrimp had a noticeably higher survival at the POD station at high temperatures, but the overall survival was only moderately higher. Amphipods, Caridean shrimp and Pisces Iiarvae exhibited more sporadic survival relative to temperature at the POD station. These three species groups also had substantially higher survival atthe POD station of 

### 35.0, 24.4 and 

### 16.7 percent, respectively, for Pisces larvae, Amphipods and Caridean shrimp.
Similar mortality rates for species groups, as observed for

### Acum'u tomu, Crab larvae and Shrimp larvae, may indicate that mortality caused by entrainment through the Condensers is similar to stress caused by passage through the dilution pumps. Atthe other extreme are the species groups with dramatically higher survival at the POD station (Pisces larvae, Amphipods and Caridean shrimp) which appear to pass through the dilution pumps with minimum stress. The remaining species groups have moderately higher survival riates at the POD station, significantly so at peak temperatures, indicating the dilution pumps do not cause significant mortality as compared to the Condensers.

- 

### 57 

### - CCI ENVIRONMENTALSERVICES, INC.The dilution pumpsappear to be iable to greatly reduce mortality of zooplankton when comparedtothe use of cooling towers. Mortality would be expected to be extensive, if not complete, for those organisms being entrained and passedthroughthe cooling towers. The high survival valuers of zooplankton entrained through the Condensers indicates that the cooling towers would cause the majority of zooplankton mortality under mostoperating conditions. It is important to note that the POD and Condenser mortalities represent the worst case conditions since the best case mortality from the control station was used to correct the survival data.

- 58 

### - CCI ENVIRONMENTALSERVICES, INC.

# APPENDIX 

# A CHAIN 

# OF CUsTODY RECORD AND 

# SAMPLE PROCESSING 

# FORMS

CCI ENVIRONMENTALSERVICES, INC.

### SERVICES, 

## INC CCI 

### ENVIRONMENTAL. 5010 US.Highway19 North Post Office

### Box 35 Palmetto,

### FL 34220-0035 Telephone: (941 )7224667 Fax: (941) 722-8384 

# CHAIN OF 

# CUSTODY RECORDCCI ENVIRONMENTALSERVICES, INC 5010 US. Highway 19 North PostOffice Box35 Palmetto,FL342204035 Telephone: (941) 7228667 Fax. (941) 7228384 Page No.

1 I I I I I I I I I ICCI ENVIRONMENTAL

### SERVICES,INC 5010US. Highway 19 North Postomce Box35 Palmetto, FL 3422Mx335 Telephone:(961) 722-6667 Far (941) 722-3384

I I 1 I I

PROCESSED BY: 'ProjedNumber: I 0125-008 ProjectTitle: 1AnclotePower Station EntrainmentStudy Clie~:Addresb;.. : lFLORlDAPOWERCOW.

Page No.

I I I I J

DESCRIPTIONOF SAMPLE:

QUALITY ASSURANCE BY. NO. OF MICRODRAWS: 

### I!

”

> 

# APPENDIX 

# B

6 

# I: TAXONOMIC 

# LIST OF SPECIES OBSERVED DURING THE ANCLOTE ENTRAINMENT STUDY i 3



### t‘



## I:

9. CCI ENVIRONMENTALSERVICES, INC.

## APPENDIX B Taxonomic List of 

### Species Observed During the Anulote Entrainment Study Phylum Platyhelminthes ClassTurbellaria Phylum Nemlertinea Phylum Annelida Class Polychaeta

Phylum Mollusca Turbelllaria sp.
Nemertinea sp.
Polyclhaetasp. Nereitlae sp. 

### Onupht sp. 

### scoloplos sp.
Class Pelecypoda (Bivalvia) Family Limitlae 

### Lima .sp. Phylum Arthropoda Class Merostomata Subclass Xiphosura 

### Limuluspolyphemus Class Crustacea Subclass Copepoda 

### Acamia toma Subclass Malacostraca Superorder Hoplorcarida Order Stomatopoda Storriatopoda sp. 

### squizila sp. Superorder Peraciarida Order Mysidacea 

### BownllanielZa sp. 

### Bowrir2aniellQ floridana 

### Brasilomysis castroi Mysitdacea sp. 

### Mysirdopsis1bahia Mysiclopsisbigelowi Taphromysisbo-' .

### 0c 0



# I 1

# 1 - B g C 0

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D



# I 1 1 I W cn m a 

# I

# I' I I W 0) m Q

### APPENDIX B 

### (CONTINUED) Order Cumacea Cumacea 

### sp. 

### Cychaspis gp. 

### Oxyurostylussmithi Order Tanaidacea 

### Hageria rcZpax Tanaidacea sp. Order lsopoda 

### Aegothoa sp. 

### Cleantisfllanicauh 

### Cynzodoce faxoni Hotea sp. 

### Eri'chsondlluJiliformis sphaerok walken Order Amphipoda 

### Acuminodeutopus nugki Arnpeliscu 

### sp

# . AmphipOda sp. 

### mea

### cahhrinensis Ca,preliidaesp. 

### Cerapus,tubuluris Carophi@msp. 

### Cymadusacompta Elnsmoplus 

### levis Erichthqnius brasiliensis 

### Gtzmmum-usmucronutus Gmmabsp. 

### Grandidierella bonneroides Ltmbos unicomis 

### Lcmbos sp. 

### Liistriel&bamardi Lysiandpsis alba Maera

### ldanae hfelitadentQta Melita sp. 

### Melita nitida 

### ddicrodeutopus sp. 

### Pardahca cuspidata PIatyischnopus sp. 

### Stenothoe minuta

### APPENDIX B 

### (CONTINUED) Superorder Eucarirda 

### 0rder Decapoda Section Penaeidea Penaleidea sp. 

### Penacws dourarum Sicyonia brevirostris Trachypen@usconstn'ctus Family Leuciferidae 

### Leuctfer 

### fioni Section Caridea Cariclea 

### sp. Family Alptieidae 

### Alphcw hekrochaelis Alpheus nomuuuzi Alpheus sp. Family Palaiemorlidae 

### Pah?monetesintermedius Pakzmonetespugio Palaemonefesvulgaris Palaemonetes

### sp

# . Periclimenesamencanus Pen'climews sp

## . Pen'clirnenks longicaudatus Family Processidae 

### Processa Sp. 

### Processa hemphilli Family Hippolytidae 

### Hippolytepleuracanthus Hippolyte ISp

# . Hippolyte Izosten'cola Tozeiumadarolinense Section Anomura

Superfamily Pagwroidea 

### Pag1urus sip. Paguroidea sp. Section Brachyura Family Poirtunidae 

### Calr!inectessapidus Family Xanthidae 

### Panopeus herbstii Meriippe mercenun'a Xarithidae sp.

### APPENDIX B 

### (CONTINUED)

- Family Pinnotheridae 

### Pinnixa sp. 

### Pinnixa sajana Family Mijildae 

### Libinliadubia Phylum Chaetognath Chaetogniatha sp. Phylum Chordata Superclass Pisces Pisc.es sp. Class Osteichthyes Family Elolphidaie 

### Megalopsptluntica Family Clupeidqe 

### DorasomU 

### sp

### . Harengula jaguana Family Engraulidae 

### Anchoa sp. 

### Amboa mktchilli Family Syingnalthidae 

### Hipivocdpus sp

# . Hippocardpuszosterae SyngnathlFsfloridae Syngnuthwscovelli Syngnathw sp. Family Centronomidae 

### ce firtropohusundecimalis Family Cairangildae 

### Ch2lorosct)mbnrs

### chrysunrs Oli,goplit$s 

### saurus GllWlx

### qppos mranx

### sip. Family Luitjanidae 

### Lwjanuslgriseus Family Gerreid~ae 

### Eu~cinOstQmus sp

# . Family Sciaenildae 

### Menticimhus sp

# . Pogonid 

### cromis Scieanidae sp. 

### Sciaerwfls ocelluta

### APPENDIX B (CONTINUED) Family Mu!gilidae 

### Mugiil sp. Family Gobiidae Gobiidae sp. 

### Gobilosomp robusturn Gobiiosomh sp

# . Family Triglidael 

### Prionotus 1sp. Triglidae sp. Family Soleidae 

### Achi’rusliheata Trinectes pp. Family Os’traciidge 

### LaaFophylt;F sp. Family Teitraod ntidae 

### Sphzeroi

# B es sp.

# APPENDIX C TAXONOMIC; LISTS 

# BY SPECIES GROUP USED 

# IN THE AFKLOTE ENTRAINMENT STUDY

CCI ENVIRONMENTALSERVICES, INC.

## APPENDIX C Taxonomic Lists by 

### Species Group Used in the Anclote Entrainment Studv Acartia tonsa (AC) Phylum Arthropoda Class Crustacea Subclass Copepoda 

### Acam'a tonsa AmrJhiDoda(AM) Phylum Arthropoda Class Crustacea Subclass Malacostraca Superorder Peracarida Order Amphipoda 

### Acumiizodeutopus naglei Ampelisca 

### sp,

# . Amphipoda sp. 

### Batea catharinensis Caprelllidae sp. 

### Cerapzllstub&lizris Corophium

## sp

# . Cymdusacompta E~LUC~US &vis 

### Erichthwnius brasiliensis 

### Gammuruswucronatus 

### Gammzrus sp. 

### Grandidierelbbonneroides Lemboir unicorn's Lemboii.sp. 

### Listriella bamrdi Lysiampsisalba Maera h e 

### Melita dentada Melita sp. 

### Melita nitida Microdeutopw sp. 

### Pardalhca cuspidata Pluqist:hnopars sp

# . Stenothoe minuta Chaetoqnatha (CHI Phylum Chaetognatha Chaetognatha sp.

### APPENDIX C (CONTINUED) Crab Larvae (CLL Phylum Arthropoda Class Crustacea Subclass Malacostraca Superorder Eucarida Order Decapoda Section Brachyura Family Portunidae 

### Callinectes sapidus zoea and megalops Family Xanthidae 

### Menippe mercenuria zoea and megalops Xanthidae sp. zoea Caridean ShrimD CCS) Phylum Arthropoda Class Crustacea Subclass Malacostraca Superorder Eucarida Order Decapoda Section Caridea Caridea sp. Family Alpheidae 

### Alpheus heterochuelis Alpheus namnni Alpheus sb. Family Paliaemooidae 

### Palaemor&es intermedius PaIaemondtespugio Palaemoqtes vulgaris Palaremo&es sp. 

### Periclimeqes americanus periclime+?ssp. 

### Periclime s 

### bngicaudatus Family Process 

# T

dae 

### Processa tip. 

### Processa kmphilli Family Hippoly$dae 

### Hipolyte~ pleuracanthus HippoEytei sp. 

### Hip polyte zostericola. Tozeumucarolinerne

### APPENDIX C 

### (CONTINUED) lsoooda

## (Is1 Phylum Arthropoda Class Crustacea Subclass Malacostraca Superorder Peracarida Order lsopoda 

### Aegoirhoa sp

## . Clearitisplmnicaudu Cymcidoce faxoni 

### Edotea sp. 

### Erichsonella 

### jiliformis 

### Sphueroma walkeri Taphromysisbowmuni Pisces Juvenile (PJL Phylum Chordata Superclass Pisces Pisces sp. Class Osteichthyes Family Eloplhidaq 

### Megadops Cttlantica Family Clupeidacj 

### Harenguhyaguana Family Engraulidae 

### Anchoa sp, 

### Anchoa rnilichilli Family Syngnathidae 

### Hippocamdus sp

## . Hippocam~us zosterae Syngirlathus jloridae Syngiwhus

### scovelli Syngilathus sp. Family Centropomidae 

### Cemropomwundecimalis Family CarimgidBe 

### Chloroscombrus

### chrysurus Oligoplites

### saurus CQranX

### hippos Family Lutjanidap 

### Lutjanus griseus Family Gerueidae 

### Eucirwstornus sp.

### APPENDIX 

### C (CONTINUED) Family Sciaenidae 

### Pogoizias cromis Scieanidae sp. Fa

### mil y 

### Muglilidaq



### Mugil sp. Family Gobiidae Gobiidae $p. 

### Gobiiosomd robustum Gbbiiosomasp. Family Triglidae 

### PriOlZOtus sp. Family Soleidae 

### Achim lineata Family Ostraciidae 

### Lasophyrs sp. Family Tetraoddntidae 

### Sphaeroides 

### sp

## . Pisces Larvae 

### (PlL Phylum Chordata Superclass Pisces Pisces sp. Class Osteichthyes


# 0 

# I 11 

# E 

# 1 8 Family Clupeidqe 

### Dorosowut sp. Family Engraulidae 

### Anchoa sp. 

### Anchoa qitchilli Family Syngnathidae 

### Syngnuthhscovelli Syngnuthlu sp. Family Centropomidae 

### Centropdpus undecimalis Family Carangidae 

### Chloroscvmbruschrysurus Caranx

### Nppos Caranx $p. Family Sciaenidae 

### Mtmicirt-hus sp. Scieanidae sp. 

### Sciaenops ocelhta Family Giobiidae Gobiidde sp. 

### Gbbiosoma robusturn

## APPENDIX C (CONTINUED) Family Trigilidae 

### Prionotus sp. Triglidae sp. Family Soleidae 

### Achirus lirleata Tn'nectes sp. PenaeidShrimD 

### (PSI Phylum Arthropoda Class Crustacea Subclass Malacostraca

- Superorder Eucarida Order Decapoda Section Penaeidea Penaeidea sp. 

### Pentaeus 

### dourarum Sicyonia Brevirostris Trachypehueuscomtrictus Family Leiuciferidae 

### Leucifer

## fihxoni Shrimr, Larvae (SLL Phylum Arthropoda Class Crustacea Subclass Malacostreica Superorder Eucarida Order Decapaida Section Periaeidea Peinaeideasp. Family Leuciferidae 

### Leiicifer 

### fmoni Section Cairidea Calridea sp. Family Palaemonidae 

### Palaemmetes vulgaris Pericliwnes sp. 

### Periclimenes longicaudatur Family Processidae 

### Processq sp. 

### Processa hemphilli Family Plippolytidae 

### Hippolyre sp

# . Hippolyre zostericola Tazemcarolinense

# APPENDIX 

# D COMPOSITE SURVIVAL DATA 

# BY SPECIES AND !SAMPLING STATION

CCI ENVIRONMENTALSERVICES, INC.

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# I I I APPENDIX E RESULTS 

# OF REGRESSION ANALYSES BETWEEN CORRECTED SURVIVAL AND CONDENSER TEMPERATURE AT THE CONDENSER AND POD STATIONS

CCI ENVIRONMENTALSERVICES, INC.ANCLOTE ENTRAINMENT STUDY

# - REGRESSION ANALYSES Corrected Survivalvs. Condenser DischargeTemperature

Acartia tonsa Amphipoda Chaetognatha Crab Larvae Caridean Shrimp lsopoda Pisces Juvenile Pisces Larvae Penaeid Shrimp Shrimp Larvae 

### 0.37 0.48 0.71 0.31 

### 0.62 0.36 0.40 0.48 0.69 

### 0.26 0.18 0.38 0.23 0.16 

### 0.52 0.31 0.07 0.02 

### 0.48 0.21



# I 1

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### INC.

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