13339 Hagers Ferry Road

Huntersville, NC 28078-7929

McGuire Nuclear Complex - MG03A2

Phone: 980-875-5245 Fax: 980-875-4349

Order Summary Report

Analytical Laboratory

Order Number: J13050469

Project Name: WWTS FGD-Routine 2013

Lab Contact: Jason C Perkins

Date: 6/27/2013

Customer Address: 3195 Pine Hall Rd

Customer Name(s): Bill Kennedy, Wayne Chapman, Melonie Martin

Mailcode: Belews Steam Station

Belews Creek, NC 28012

Phone: 980-875-5348

Report Authorized By:

(Signature)

Program Comments:

Please contact the Program Manager (Jason C Perkins) with any questions regarding this report.

Data Flags & Calculations:

Any analytical tests or individual analytes within a test flagged with a Qualifier indicate a deviation from the method quality 

system or quality control requirement. The qualifier description is found at the end of the Certificate of Analysis (sample results) 

under the qualifiers heading. All results are reported on a dry weight basis unless otherwise noted. Subcontracted data 

included on the Duke Certificate of Analysis is to be used as information only. Certified vendor results can be found in the 

subcontracted lab final report. Duke Energy Analytical Laboratory subcontracts analyses to other vendor laboratories that have 

been qualified by Duke Energy to perform these analyses except where noted.

Data Package:

This data package includes analytical results that are applicable only to the samples described in this narrative. An estimation of 

the uncertainty of measurement for the results in the report is available upon request. This report shall not be reproduced, except 

in full, without the written consent of the Analytical Laboratory. Please contact the Analytical laboratory with any questions. The 

order of individual sections within this report is as follows:

Job Summary Report, Sample Identification, Technical Validation of Data Package, Analytical Laboratory Certificate of Analysis, 

Analytical Laboratory QC Reports, Sub-contracted Laboratory Results, Customer Specific Data Sheets, Reports & 

Documentation, Customer Database Entries, Test Case Narratives, Chain of Custody (COC)

Certification:

The Analytical Laboratory holds the following State Certifications : North Carolina (DENR) Certificate #248, South Carolina 

(DHEC) Laboratory ID # 99005. Contact the Analytical Laboratory for definitive information about the certification status of 

specific methods. 

Jason C Perkins

Page 1 of 16
Sample ID's & Descriptions:

Sample ID Plant/Station

Collection 

Date and Time Collected By Sample Description

2013012126 BELEWS 28-May-13 7:30 AM TRAVIS THORNTON FGD Purge Eff 

2013012127 BELEWS 28-May-13 7:35 AM TRAVIS THORNTON EQ Tank Eff 

2013012128 BELEWS 28-May-13 7:40 AM TRAVIS THORNTON BioReactor 1 Inf 

2013012129 BELEWS 28-May-13 7:45 AM TRAVIS THORNTON BioReactor 2 Inf 

2013012130 BELEWS 28-May-13 7:50 AM TRAVIS THORNTON BioReactor 2 Eff 

2013012131 BELEWS 28-May-13 8:10 AM TRAVIS THORNTON Filter Blk 

2013012132 BELEWS 21-May-13 10:30 AM CPK TRIP BLANK 

7 Total Samples

Page 2 of 16
COC and .pdf report are in agreement with sample totals 

and analyses (compliance programs and procedures).

All Results are less than the laboratory reporting limits.

All laboratory QA/QC requirements are acceptable.

Yes No

Technical Validation Review

Checklist:

Yes No

Yes No

Report Sections Included:

Job Summary Report Sub-contracted Laboratory Results

Sample Identification Customer Specific Data Sheets, Reports, & Documentation

Technical Validation of Data Package Customer Database Entries

Analytical Laboratory Certificate of Analysis

Analytical Laboratory QC Report

Chain of Custody

Reviewed By: DBA Account Date: 6/27/2013

Electronic Data Deliverable (EDD) Sent Separately

Page 3 of 16
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J13050469

2013012126

Collection Date: 28-May-13 7:30 AM

Site: FGD Purge Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

NITRITE + NITRATE (COLORIMETRIC)

Nitrite + Nitrate (Colorimetric) 14 mg-N/L 0.1 10 EPA 353.2 06/03/2013 12:02 BGN9034

INORGANIC IONS BY IC

Bromide 110 mg/L 5 50 EPA 300.0 06/03/2013 15:55 JAHERMA

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) 248 ug/L 5 100 EPA 245.1 05/31/2013 12:47 AGIBBS

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 224 mg/L 0.5 10 EPA 200.7 06/07/2013 11:54 DJSULL1

DISSOLVED METALS BY ICP-MS

Selenium (Se) 130 ug/L 10 10 EPA 200.8 06/12/2013 11:24 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) 249 ug/L 10 10 EPA 200.8 06/12/2013 13:47 KRICHAR

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 06/12/2013 13:47 KRICHAR

Chromium (Cr) 261 ug/L 10 10 EPA 200.8 06/12/2013 13:47 KRICHAR

Copper (Cu) 128 ug/L 10 10 EPA 200.8 06/12/2013 13:47 KRICHAR

Nickel (Ni) 227 ug/L 10 10 EPA 200.8 06/12/2013 13:47 KRICHAR

Selenium (Se) 3410 ug/L 10 10 EPA 200.8 06/12/2013 13:47 KRICHAR

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 06/12/2013 13:47 KRICHAR

Zinc (Zn) 275 ug/L 10 10 EPA 200.8 06/12/2013 13:47 KRICHAR

SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete Vendor Method V_AS&C

2013012127

Collection Date: 28-May-13 7:35 AM

Site: EQ Tank Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) 179 ug/L 2.5 50 EPA 245.1 05/31/2013 12:50 AGIBBS

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 226 mg/L 0.5 10 EPA 200.7 06/07/2013 11:58 DJSULL1

DISSOLVED METALS BY ICP-MS

Selenium (Se) 73.0 ug/L 10 10 EPA 200.8 06/12/2013 11:27 KRICHAR

Page 4 of 16
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J13050469

2013012127

Collection Date: 28-May-13 7:35 AM

Site: EQ Tank Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) 171 ug/L 10 10 EPA 200.8 06/12/2013 13:50 KRICHAR

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 06/12/2013 13:50 KRICHAR

Chromium (Cr) 220 ug/L 10 10 EPA 200.8 06/12/2013 13:50 KRICHAR

Copper (Cu) 102 ug/L 10 10 EPA 200.8 06/12/2013 13:50 KRICHAR

Nickel (Ni) 212 ug/L 10 10 EPA 200.8 06/12/2013 13:50 KRICHAR

Selenium (Se) 2420 ug/L 10 10 EPA 200.8 06/12/2013 13:50 KRICHAR

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 06/12/2013 13:50 KRICHAR

Zinc (Zn) 218 ug/L 10 10 EPA 200.8 06/12/2013 13:50 KRICHAR

2013012128

Collection Date: 28-May-13 7:40 AM

Site: BioReactor 1 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

NITRITE + NITRATE (COLORIMETRIC)

Nitrite + Nitrate (Colorimetric) 17 mg-N/L 0.1 10 EPA 353.2 06/03/2013 12:03 BGN9034

Mercury by EPA 200.8 - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete ug/l Vendor Method V_AS&C

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 207 mg/L 0.5 10 EPA 200.7 06/07/2013 12:01 DJSULL1

DISSOLVED METALS BY ICP-MS

Selenium (Se) 78.9 ug/L 10 10 EPA 200.8 06/12/2013 11:30 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 10 ug/L 10 10 EPA 200.8 06/12/2013 13:54 KRICHAR

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 06/12/2013 13:54 KRICHAR

Chromium (Cr) < 10 ug/L 10 10 EPA 200.8 06/12/2013 13:54 KRICHAR

Copper (Cu) < 10 ug/L 10 10 EPA 200.8 06/12/2013 13:54 KRICHAR

Nickel (Ni) 15.7 ug/L 10 10 EPA 200.8 06/12/2013 13:54 KRICHAR

Selenium (Se) 80.9 ug/L 10 10 EPA 200.8 06/12/2013 13:54 KRICHAR

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 06/12/2013 13:54 KRICHAR

Zinc (Zn) < 10 ug/L 10 10 EPA 200.8 06/12/2013 13:54 KRICHAR

SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete Vendor Method V_AS&C

Page 5 of 16
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J13050469

2013012129

Collection Date: 28-May-13 7:45 AM

Site: BioReactor 2 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

Mercury by EPA 200.8 - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete ug/l Vendor Method V_AS&C

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 200 mg/L 0.5 10 EPA 200.7 06/07/2013 12:05 DJSULL1

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 10 ug/L 10 10 EPA 200.8 06/12/2013 13:57 KRICHAR

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 06/12/2013 13:57 KRICHAR

Chromium (Cr) < 10 ug/L 10 10 EPA 200.8 06/12/2013 13:57 KRICHAR

Copper (Cu) < 10 ug/L 10 10 EPA 200.8 06/12/2013 13:57 KRICHAR

Nickel (Ni) < 10 ug/L 10 10 EPA 200.8 06/12/2013 13:57 KRICHAR

Selenium (Se) 12.8 ug/L 10 10 EPA 200.8 06/12/2013 13:57 KRICHAR

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 06/12/2013 13:57 KRICHAR

Zinc (Zn) < 10 ug/L 10 10 EPA 200.8 06/12/2013 13:57 KRICHAR

2013012130

Collection Date: 28-May-13 7:50 AM

Site: BioReactor 2 Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

NITRITE + NITRATE (COLORIMETRIC)

Nitrite + Nitrate (Colorimetric) < 0.01 mg-N/L 0.01 1 EPA 353.2 06/03/2013 12:04 BGN9034

INORGANIC IONS BY IC

Bromide 100 mg/L 5 50 EPA 300.0 06/03/2013 16:14 JAHERMA

Mercury by EPA 200.8 - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete ug/l Vendor Method V_AS&C

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 203 mg/L 0.5 10 EPA 200.7 06/07/2013 12:08 DJSULL1

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 5 ug/L 5 5 EPA 200.8 06/12/2013 14:01 KRICHAR

Cadmium (Cd) < 5 ug/L 5 5 EPA 200.8 06/12/2013 14:01 KRICHAR

Chromium (Cr) < 5 ug/L 5 5 EPA 200.8 06/12/2013 14:01 KRICHAR

Copper (Cu) < 5 ug/L 5 5 EPA 200.8 06/12/2013 14:01 KRICHAR

Nickel (Ni) < 5 ug/L 5 5 EPA 200.8 06/12/2013 14:01 KRICHAR

Selenium (Se) < 5 ug/L 5 5 EPA 200.8 06/12/2013 14:01 KRICHAR

Silver (Ag) < 5 ug/L 5 5 EPA 200.8 06/12/2013 14:01 KRICHAR

Zinc (Zn) < 5 ug/L 5 5 EPA 200.8 06/12/2013 14:01 KRICHAR

Page 6 of 16
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J13050469

2013012130

Collection Date: 28-May-13 7:50 AM

Site: BioReactor 2 Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete Vendor Method V_AS&C

TOTAL DISSOLVED SOLIDS

TDS 17000 mg/L 25 1 SM2540C 06/03/2013 15:03 JDTALLE

2013012131

Collection Date: 28-May-13 8:10 AM

Site: Filter Blk 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

DISSOLVED METALS BY ICP-MS

Selenium (Se) < 1 ug/L 1 1 EPA 200.8 06/12/2013 10:38 KRICHAR

2013012132

Collection Date: 21-May-13 10:30 AM

Site: TRIP BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

TOTAL RECOVERABLE METALS BY ICP

Boron (B) < 0.05 mg/L 0.05 1 EPA 200.7 06/07/2013 11:47 DJSULL1

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 1 ug/L 1 1 EPA 200.8 06/12/2013 13:40 KRICHAR

Cadmium (Cd) < 1 ug/L 1 1 EPA 200.8 06/12/2013 13:40 KRICHAR

Chromium (Cr) < 1 ug/L 1 1 EPA 200.8 06/12/2013 13:40 KRICHAR

Copper (Cu) < 1 ug/L 1 1 EPA 200.8 06/12/2013 13:40 KRICHAR

Nickel (Ni) < 1 ug/L 1 1 EPA 200.8 06/12/2013 13:40 KRICHAR

Selenium (Se) < 1 ug/L 1 1 EPA 200.8 06/12/2013 13:40 KRICHAR

Silver (Ag) < 1 ug/L 1 1 EPA 200.8 06/12/2013 13:40 KRICHAR

Zinc (Zn) < 1 ug/L 1 1 EPA 200.8 06/12/2013 13:40 KRICHAR

Page 7 of 16
 

June 11, 2013 

Jay Perkins 

Duke Energy Analytical Laboratory 

Mail Code MGO3A2 (Building 7405) 

13339 Hagers Ferry Rd. 

Huntersville, NC 28078 

(704) 875-5245 

Project: Belews - FGD WWTS (Bi-Monthly Routine 2013) (LIMS #J13050469) 

Mr. Perkins,

Attached is the report associated with four (4) aqueous samples submitted for total mercury and 

selenium speciation analysis on May 30, 2013. The samples were received in a sealed cooler at

-0.3°C on May 31, 2013. Selenium speciation analysis was performed via ion chromatography 

inductively coupled plasma collision reaction cell mass spectrometry (IC-ICP-CRC-MS). 

Mercury quantitation was performed via cold vapor inductively coupled plasma mass 

spectrometry (CV-ICP-MS). Any issues associated with the analysis are addressed in the 

following report. 

If you have any questions, please feel free to contact me at your convenience. 

 

 Sincerely, 

 

 Russell Gerads 

Vice President 

 Applied Speciation and Consulting, LLC 

 

Page 8 of 16
Applied Speciation and Consulting, LLC 

Report prepared for: 

Jay Perkins 

Duke Energy Analytical Laboratory 

Mail Code MGO3A2 (Building 7405) 

13339 Hagers Ferry Rd. 

Huntersville, NC 28078 

Project: Belews - FGD WWTS (Bi-Monthly Routine 2013) (LIMS #J13050469) 

June 11 2013 

1. Sample Reception 

Three (3) aqueous samples in 125mL HDPE bottles (provided by Applied Speciation and 

Consulting) were submitted for selenium speciation analysis on May 30, 2013. Three (3) 

additional samples in 40ml borosilicate glass bottles (provided by Applied Speciation and 

Consulting) were submitted for total mercury quantitation. All samples were received in 

acceptable condition on May 31, 2013 in a sealed container at -0.3°C.

All samples were received in a laminar flow clean hood, void of trace metals contamination 

and ultra-violet radiation, and were designated discrete sample identifiers. The 40mL 

borosilicate glass vials submitted for total mercury were preserved with bromine 

monochloride (BrCl) solution. The resulting samples were stored in a secure polyethylene 

container, known to be free from trace metals contamination, until the analyses could be 

performed. 

An aliquot of each sample requiring selenium speciation evaluation was filtered (0.45μm) 

and each filtrate was stored in a secure, monitored cryofreezer (maintained at a temperature 

of -80°C) until selenium speciation analysis could be performed via ion chromatography 

inductively coupled plasma collision reaction cell mass spectrometry (IC-ICP-CRC-MS). 

2. Sample Preparation 

All sample preparation is performed in laminar flow clean hoods known to be free from trace 

metals contamination. All applied water for dilutions and sample preservatives are 

monitored for contamination to account for any biases associated with the sample results. 

Total Mercury Quantitation by CV-ICP-MS All samples and preparation blanks for total 

mercury quantitation were preserved with 2% (v/v) BrCl. The resulting samples were 

analyzed for mercury via cold vapor inductively coupled plasma mass spectrometry (CVICP-MS). 

Page 9 of 16
Selenium Speciation Analysis by IC-ICP-CRC-MS Prior to analysis, an aliquot of each 

sample was filtered with a syringe filter (0.45μm) and injected directly into a sealed 

autosampler vial. No further sample preparation was performed as any chemical alteration of 

a sample may shift the equilibrium of the system, resulting in changes in speciation ratios. 

3. Sample Analysis 

All sample analysis is preceded by a minimum of a five-point calibration curve spanning the 

entire concentration range of interest. Calibration curves are performed at the beginning of 

each analytical day. All calibration curves, associated with each species of interest, are 

standardized by linear regression resulting in a response factor. All sample results are 

instrument blank corrected to account for any operational biases associated with the 

analytical platform. 

Prior to sample analysis, all calibration curves are verified using second source standards 

which are identified as initial calibration verification standards (ICV). 

Ongoing instrument performance is identified by the analysis of continuing calibration 

verification standards (CCV) and continuing calibration blanks (CCB) at a minimum interval 

of every ten analytical runs. 

Total Mercury Quantitation by CV-ICP-MS The sample fractions for total mercury 

quantitation were analyzed by cold vapor inductively coupled plasma mass spectrometry 

(CV-ICP-MS) on May 31, 2013. Aliquots of each sample are reacted with a reductant in-line 

and transported to a gas-liquid separator. The volatile elemental mercury that is formed is 

then swept by a stream of argon gas into a radio frequency (RF) plasma where energytransfer processes cause desolvation, atomization, and ionization. The ions are extracted 

from the plasma through a differentially-pumped vacuum interface and separated on the basis 

of their mass-to-charge ratio (m/z) by a mass spectrometer. A solid-state detector detects 

ions transmitted through the mass analyzer and the resulting current is processed by a data 

handling system. 

Selenium Speciation Analysis by IC-ICP-CRC-MS Each sample for selenium speciation 

analysis was analyzed by ion chromatography inductively coupled plasma collision reaction 

cell mass spectrometry (IC-ICP-CRC-MS) on June 7, 2013. An aliquot of each sample is

injected onto an anion exchange column and mobilized by a basic (pH > 7) gradient. The 

eluting selenium species are then introduced into a radio frequency (RF) plasma where 

energy-transfer processes cause desolvation, atomization, and ionization. The ions are 

extracted from the plasma through a differentially-pumped vacuum interface and travel 

through a pressurized chamber (CRC) containing a reaction gas which preferentially reacts 

with interfering ions of the same target mass to charge ratios (m/z). A solid-state detector 

detects ions transmitted through the mass analyzer and the resulting current is processed by a 

data handling system. 

Page 10 of 16
Retention times for each eluting species are compared to known standards for species 

identification. 

4. Analytical Issues 

The overall analyses went well and no significant analytical issues were encountered. All 

quality control parameters associated with these samples were within acceptance limits.

A miscommunication between the ion chromatography unit and the eluent pump caused a 

delay in the eluent gradient during the analysis of the matrix spike duplicate performed on 

the sample identified as batch QC. Consequently the retention time for the selenocyanate 

peak in the matrix spike duplicate sample was shifted to a larger value (from approximately 

10.2 minutes to 11.6 minutes). This shift was confirmed by measurement of an internal 

standard (roxarsone), which typically falls within the same late eluting retention time range. 

The roxarsone peak experienced a similar retention time shift due to the delay in the eluent 

gradient. The selenocyanate peak occurring at 11.6 minutes in the matrix spike sample was 

integrated and the value produced an acceptable selenocyanate recovery. 

The estimated method detection limits (eMDLs) for selenite, selenate, and selenocyanate are 

generated from replicate analyses of the lowest standard in the calibration curve. Not all 

selenium species are present in preparation blanks; therefore, eMDL calculations based on 

preparation blanks are artificially biased low. 

The eMDL for methylseleninic acid and selenomethionine is calculated from the average 

eMDL of selenite, selenate, and selenocyanate. The calibration does not contain 

methylseleninic acid or selenomethionine due to impurities in these standards which would 

bias the results for other selenium species. 

The eMDL for mercury has been calculated using the standard deviation of the preparation 

blanks preserved and analyzed concurrently with the submitted samples. 

If you have any questions or concerns regarding this report, please feel free to contact me. 

Sincerely, 

Russell Gerads 

Vice President 

Applied Speciation and Consulting, LLC 

Page 11 of 16
Sample Results

Sample ID Total Hg Se(IV) Se(VI) SeCN MeSe(IV) SeMe

Unknown Se 

Species (n)

FGD Purge Eff NR 92.9 58.2 ND (< 5.0) ND (< 5.6) ND (< 5.6) 0 (0)

BioReactor 1 Inf 0.101 28.2 52.7 ND (< 1.2) ND (< 1.4) ND (< 1.4) 0 (0)

BioReactor 2 Inf 0.0156 NR NR NR NR NR NR

BioReactor 2 Eff 0.0045 ND (< 1.3) ND (< 1.6) ND (< 1.2) ND (< 1.4) ND (< 1.4) 0 (0)

All results reflect the applied dilution and are reported in μg/L

NR = Analysis not requested

ND = Not detected at the applied dilution

SeCN = Selenocyanate

MeSe(IV) = Methylseleninic acid

SeMe = Selenomethionine

Unknown Se Species = Total concentration of all unknown Se species observed by IC-ICP-MS

n = number of unknown Se species observed

Report Generated by: Russell Gerads

Applied Speciation and Consulting, LLC

Total Mercury & Selenium Speciation Results for Duke Energy

Project Name: Belews - FGD WWTS (Bi-Monthly Routine 2013)

Contact: Jay Perkins

Date: June 11, 2013

LIMS #J13050469

Page 12 of 16
Quality Control Summary - Preparation Blank Summary

Analyte (μg/L) PBW1 PBW2 PBW3 PBW4 Mean StdDev eMDL* eMDL 5x eMDL 250x eMDL 1000x

Hg 0.0007 0.0016 0.0016 0.0016 0.0014 0.0005 0.0003 0.0014 - -

Se(IV) 0.000 0.000 0.144 0.000 0.036 0.072 0.005 - 1.3 5.4

Se(VI) 0.000 0.000 0.000 0.000 0.000 0.000 0.006 - 1.6 6.4

SeCN 0.000 0.000 0.000 0.000 0.000 0.000 0.005 - 1.2 5.0

MeSe(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.006 - 1.4 5.6

SeMe 0.000 0.000 0.049 0.000 0.012 0.024 0.006 - 1.4 5.6

eMDL = Estimated Method Detection Limit

*Please see narrative regarding eMDL calculations

Quality Control Summary - Certified Reference Materials

Analyte (μg/L) CRM True Value Result Recovery

Hg NIST 1641d 1568 1580 100.8

Se(IV) LCS 4.79 5.23 109.2

Se(VI) LCS 4.74 4.87 102.8

SeCN LCS 4.46 4.75 106.6

MeSe(IV) LCS 3.24 3.37 104.1

SeMe LCS 4.66 4.56 97.9

Applied Speciation and Consulting, LLC

Total Mercury & Selenium Speciation Results for Duke Energy

Project Name: Belews - FGD WWTS (Bi-Monthly Routine 2013)

Contact: Jay Perkins

LIMS #J13050469

Date: June 11, 2013

Report Generated by: Russell Gerads

Page 13 of 16
Quality Control Summary - Matrix Duplicates

Analyte (μg/L) Sample ID Rep 1 Rep 2 Mean RPD

Hg Batch QC 0.0682 0.0671 0.0677 1.6

Se(IV) Batch QC ND (< 1.3) ND (< 1.3) NC NC

Se(VI) Batch QC ND (< 1.6) ND (< 1.6) NC NC

SeCN Batch QC ND (< 1.2) ND (< 1.2) NC NC

MeSe(IV) Batch QC ND (< 1.4) ND (< 1.4) NC NC

SeMe Batch QC ND (< 1.4) ND (< 1.4) NC NC

ND = Not detected at the applied dilution

NC = Value was not calculated due to one or more concentrations below the eMDL

Quality Control Summary - Matrix Spike/ Matrix Spike Duplicate

Analyte (μg/L) Sample ID Spike Conc MS Result Recovery Spike Conc MSD Result Recovery RPD

Hg Batch QC 2.000 2.159 104.6 2.000 2.169 105.1 0.4

Se(IV) Batch QC 1390 1535 110.4 1390 1530 110.1 0.3

Se(VI) Batch QC 1261 1377 109.2 1261 1358 107.7 1.4

SeCN * Batch QC 1144 1232 107.7 1144 1222 106.9 0.8

* Please see narrative regarding MSD selenocyanate result.

Applied Speciation and Consulting, LLC

Total Mercury & Selenium Speciation Results for Duke Energy

Project Name: Belews - FGD WWTS (Bi-Monthly Routine 2013)

Contact: Jay Perkins

LIMS #J13050469

Date: June 11, 2013

Report Generated by: Russell Gerads

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