Bioassays of 

Harbor Branch Oceanographic Institute 

Reverse Osmosis Concentrate Disposal Facility

St. Lucie County 

NPDES #FL0139912 

Sampled November 28, 2005 

March 2006

Biology Section 

Bureau of Laboratories 

Division of Resource Assessment and Management 

Quality Manual No. 870346G 

NELAC Certification No. E31780
1

Harbor Branch Oceanographic Institute Reverse Osmosis Concentrate Disposal Facility, 5600 US 

Highway 1 North, Fort Pierce, St. Lucie County, Florida, NPDES #FL0139912. Effluent samples 

for this facility were collected on November 28, 2005. 

Introduction 

Harbor Branch Oceanographic Institute Reverse Osmosis Concentrate Disposal Facility is a 

reverse osmosis (RO) water treatment plant which supplies potable water to the Harbor Branch 

Oceanographic Institute. The facility uses the surficial aquifer for its supply water. The facility has 

a design flow of 12,000 gallons per day (GPD) and had an annual average daily flow (AADF) of 

4,420 GPD (December 2004 to November 2005) through internal Outfall I-002. The RO 

concentrate is not treated prior to discharge into a stormwater retention pond. Dilution occurs 

within the stormwater pond prior to discharge of effluent from the pond through Outfall D-001 to 

Class III freshwater wetlands adjacent to the Class III Marine waters of the Indian River Lagoon. 

Flow is not monitored at Outfall D-001. 

The facility is not under any Administrative or Consent Orders. The facility has no toxicity test 

requirements. There have been no permit violations or plant upsets within the past year. During 

the past year, copper values at D-001 ranged from 2.2 μg/L to 7.4 μg/L. These concentrations 

were attributed to accumulation of copper in the pond sediment from prior treatment of the pond 

with copper sulfate (to control algal growth) and were not attributed to the RO plant (facility 

information provided by Terry Davis of FDEP Southeast District). 

Methods 

Samples were collected from Outfall D-001 and Internal Outfall I-002 by Terry Davis (FDEP 

Southeast District) following DEP-SOP-001/01 FS 2400 Wastewater Sampling. All DEP SOP’s 

are available on the web at: http://www.floridadep.org/labs/qa/sops.htm. 

The acute screen toxicity tests and algal growth potential test were performed following internal 

DEP SOP’s TA07_01, TA07_02, and TA08_01 through TA08_09. All internal DEP SOP’s are 

available on the web at: http://www.floridadep.org/labs/cgi-bin/sop/biosop.asp. The Department 

uses the 48-hour acute screening toxicity test to provide reasonable assurance that the facility 

does not adversely affect waters of the state. 

The primary bioassays for this facility were performed on the effluent sample collected from 

Outfall D-001, the permitted discharge from the stormwater retention pond. Two additional 

bioassays were performed on a sample of effluent collected from Outfall I-002, the internal outfall 

discharging RO concentrate into the stormwater retention pond. Bioassays were performed with 

effluent from both outfalls to allow us to distinguish between potential toxicity in the main effluent 

versus the stormwater retention pond. The EPA suite of chemical analyses was performed on 

effluent samples collected from Outfall D-001. Metal analyses only were performed on effluent 

samples collected from Outfall I-002. The algal growth potential (AGP) sample was collected from 

Outfall D-001. 

Toxicity Test Results 

Tests were performed November 29 – December 1, 2005. 

Outfall D-001 

Ceriodaphnia dubia 48-hour acute screening bioassay – LC50 > 100%, 5% mortality* in 100% 

 sample at 48 hours 

Cyprinella leedsi 48-hour acute screening bioassay – No Result Reported (Control Failure) 
2

Internal Outfall I-002 

Ceriodaphnia dubia 48-hour acute screening bioassay – LC50 < 100%, 65% mortality* in 100% 

 sample at 48 hours 

Cyprinella leedsi 48-hour acute screening bioassay – LC50 > 100%, 10% mortality* in 100% 

 sample at 48 hours 

The bioassay sample from Outfall D-001 was not acutely toxic to the Ceriodaphnia dubia test 

organisms. The Cyprinella leedsi test for this sample was invalidated due to the control failure 

(15% control mortality, 10% mortality allowed). The bioassay sample from Outfall I-002 was 

acutely toxic to the Ceriodaphnia dubia test organisms but was not acutely toxic to the Cyprinella 

leedsi test organisms. 

Ion imbalance is suspected to be the primary contributor to the mortality in the Ceriodaphnia 

dubia bioassay. The effluent (I-002) calcium concentration (402 mg/L) is well within the range 

reported to produce acute effects to Ceriodaphnia dubia and similar species (USEPA, 2006. 

ECOTOX: Ecotoxicology Database). 

See Appendix 1 for bioassay bench sheets. 

*NOTE: In a 48-hour acute screening test of 100% effluent, mortality of <20% provides 

reasonable assurance that the effluent meets the acute toxicity criterion of Florida’s Surface 

Water Quality Standards (Rule 62-302.500 (1)(a)4, F.A.C.). Mortality between 20-50% mortality 

indicates low to moderate levels of toxicity, and further action may be required. Mortality of >50% 

provides reasonable assurance that the effluent fails to meet the minimum requirement to 

discharge to waters of the state (Rule 62-4.244(3)(a), F.A.C.). 

Algal Growth Potential 

The D-001 effluent AGP was 4.19 mg dry wt/L of the freshwater unicellular green alga, 

Pseudokirchneriella subcapitata, formerly known as Selenastrum capricornutum. Raschke and 

Shultz (1987) found that AGP values above 5.0 mg dry weight/L represent a “problem” threshold 

for fresh receiving waters, implying nutrient enrichment. The analytical chemistry suggests that 

the D-001 effluent is nitrogen-limited. The predicted AGP based on inorganic nitrogen 

concentrations was 1.03 (±20%) mg dry wt/L (Table 1; Miller et al. 1978). 

See Table 1 for AGP results. 

Chemistry Results 

Total residual chlorine was not measured in the field and was not detected in either of the 

bioassay samples in the laboratory. Total ammonia was not detected in the bioassay sample 

collected from Outfall D-001 but was detected in the bioassay sample collected from Outfall I-002 

at a concentration of 0.0829 mg/L. The total ammonia concentration in the sample collected from 

Outfall D-001 and preserved for chemical analysis was 0.012 mg/L. This value is between the 

laboratory method detection limit (MDL) and the laboratory practical quantitation limit (PQL). 

Based on the pH, salinity, and temperature of Outfall D-001 effluent as collected, the calculated 

unionized ammonia concentration was < 0.02 mg/L. 

Copper and iron were detected in the Outfall D-001 effluent at levels that comply with Class III 

freshwater quality criteria. Arsenic, lead, nickel, and zinc were detected between the laboratory 

MDL and PQL. Aluminum was detected in the Outfall D-001 effluent. 

Chromium III was detected in the Outfall I-002 effluent at a level that complied with Class III 

freshwater quality criteria. Copper, lead, and nickel were detected at levels between the MDL and 
3

PQL. Aluminum was detected in Outfall I-002 effluent. Concentrations of chromium-III and zinc 

were greater at Outfall I-002 than at Outfall D-001. 

See Table 2 for results of analytes detected in the effluent, and corresponding limits. 

See Appendix 2 for a complete list of chemical analyses performed. 

Conclusion 

The effluent sample collected from Outfall D-001 for this facility on November 28, 2005, was not 

acutely toxic to the Ceriodaphnia dubia test species during the acute screen 48-hour bioassay. 

The Cyprinella leedsi test was invalidated due to excessive control organism mortality. The 

freshwater AGP result for the effluent was less than the "problem” threshold for fresh receiving 

waters. Effluent water quality samples did not violate any permit conditions. 

The effluent sample collected from Outfall I-002 for this facility on November 28, 2005, was 

acutely toxic to the Ceriodaphnia dubia test species but was not acutely toxic to the Cyprinella 

leedsi test organisms during the acute screen 48-hour bioassays. The cause of the toxicity was 

attributed in part to the calcium concentration in the sample. 

Literature Cited 

Miller, W. E., T. E. Maloney, and J. C. Greene. 1978. The Selenastrum capricornutum Printz 

algal assay bottle test. EPA-600/9-78-018. U. S. EPA, Cincinnati, Ohio. 126 p. 

Raschke, R. L. and D. A. Schultz. 1987. The use of the algal growth potential test for data 

assessment. J. Wat. Poll. Cont. Fed. 59(4): 222-227. 
4

Location AGP 

(measured)

Predicted AGP 

(TSIN) ± 20%

Predicted AGP 

(TN) ± 20%

Inorganic N:P 

ratio Total N:P ratio

Outfall D-001 4.16 1.03 ± 0.206 24.13 ± 4.826 0.1 1.81

Table 1. Measured and predicted algal growth potential (AGP; mg dry wt/L) of the freshwater species 

Psuedokirchneriella subcapitata for total soluble nitrogen (TSIN) and total nitrogen (TN) limitation and 

ratios of nitrogen to phosphorus for samples collected from Harbor Branch Oceanographic Institute 

Reverse Osmosis Concentrate Disposal Facility on November 28, 2005.
5

Harbor Branch Oceanographic 

Institute NPDES# FL0139912

Class III 

Freshwater 

Stds

Effluent 

Limits 

I-002

Effluent 

Limits 

D-001

Outfall 

I-002

Outfall 

D-001

None Detected - - - - -

Aluminum - - - 28 26 A

Arsenic ≤ 50 - - 4 U 6.4 I

Cadmium ≤ 3.4 b - - 0.25 U 0.05 U

Calcium (mg/L) - - - 402 57.8 A

Chromium-III ≤ 268.2 b - - 6 1 U

Copper ≤ 30.5 b - Report 2.6 I 4.13 A

Iron ≤ 1,000 - - 1,000 81 A

Lead ≤ 18.6 b - - 0.96 I 0.11 I

Magnesium (mg/L) - - - 13.3 4.4 A

Nickel ≤ 168.5 b - - 4 I 1.6 I

Selenium ≤ 5 - - 2.5 eU 1 eU

Silver ≤ 0.07 - - 0.12 U 0.025 U

Zinc ≤ 387.8 b - - 96 4 I

Ortho-phosphate - - - - 0.28

Total Phosphorus - Report Report - 0.35

Ammonia - - - - 0.012 I

Unionized Ammonia ≤ 0.02 - - - < 0.02 c

Nitrate+Nitrite - - - - 0.015 f

Total Kjeldahl Nitrogen - - - - 0.62

Organic Nitrogen - - - - 0.61 c

Total Nitrogen - - - - 0.64 c

pH (S.U.) 6.0-8.5 6.0-8.5 Report 6.5 7.5

Conductivity (umhos/cm) 1,275 - - 2,008 474

Temperature (C) - - - 25.9 23.7

Dissolved Oxygen (mg/L) ≥ 5.0 - - - 9.2

Chlorophyll a (μg/L) - - - - 1.8 I

Phaeophytin a (μg/L) - - - - 0 J

Flow (MGD) - ≤ 0.0075 m - 0.0042a -

Hardness (mg/L) - - - 1,058.6 c 162.4 c

Chloride (mg/L) - Report Report - -

Value exceeds the Class III Freshwater Water Quality Criteria (62-302, F.A.C.) and/or Effluent Limits

a - Annual average

c - Calculated value

e - Selenium was detected in the field blank at a concentration of 0.52 μg/L I 

f - Nitrate+nitrite was detected in the field blank at a concentration of 0.046 mg/L 

m - Monthly average

A - Value reported is the mean of two or more determinations

I - The reported value is between the laboratory method detection limit and the laboratory practical quantitation limit.

J - Estimated value

U - Material analyzed for but not detected; value reported is the minimum detection limit

General Physical and Chemical Parameters

Table 2. Effluent limits, Class III Freshwater Criteria, and chemical data for samples collected from 

Harbor Branch Oceanographic Institute Reverse Osmosis Concentrate Disposal Facility on 

November 28, 2005.

Organic Constituents (μg/L)

Metals (μg/L unless otherwise noted)

Nutrients (mg/L)
6

Appendix 1. Bench Sheets 
7

Appendix 1. Bench Sheets (continued) 
8

Appendix 1. Bench Sheets (continued) 
9

Appendix 1. Bench Sheets (continued) 
10

Appendix 1. Bench Sheets (continued) 
11

Appendix 1. Bench Sheets (continued) 
12

Appendix 1. Bench Sheets (continued) 
13

Appendix 1. Bench Sheets (continued) 
14

Appendix 1. Bench Sheets (continued) 
15

Appendix 1. Bench Sheets (continued) 
16

Appendix 2. Chemical Analyses performed on the effluent from the Harbor Branch OceanographOsmosis Concentrate Disposal Facility Outfall D-001 and Internal Outfall I-002, sampled on Nove

Date Sampled Field ID Analysis Group Component Result Units 11/28/2005 11:20 Outfall D-001 Bio-AGP/LimNut Algal Growth Potential 4.16 mg DryWt/11/28/2005 11:20 Outfall D-001 Bio-Chl-a Chlorophyll-A, Monochromatic, Water 1.8 ug/L 11/28/2005 11:20 Outfall D-001 Bio-Chl-a Phaeophytin-A, Monochromatic, Water 0 ug/L 11/28/2005 11:20 Outfall D-001 Bio-Toxicology Bioassay-Acute-Screen-FW-C.dubia, LC50 100 LC50 11/28/2005 11:20 Outfall D-001 Bio-Toxicology Bioassay-Acute-Screen-FW-Fish, LC50 No Result LC50 11/28/2005 10:40 Effluent I-002 Bio-Toxicology Bioassay-Acute-Screen-FW-C.dubia, LC50 100 LC50 11/28/2005 10:40 Effluent I-002 Bio-Toxicology Bioassay-Acute-Screen-FW-Fish, LC50 100 LC50 11/28/2005 11:20 Outfall D-001 BNA-Water 1,2,4-Trichlorobenzene 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water 1,2-Dichlorobenzene 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water 1,3-Dichlorobenzene 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water 1,4-Dichlorobenzene 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water 2,4,6-Trichlorophenol 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water 2,4-Dichlorophenol 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water 2,4-Dimethylphenol 48 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water 2,4-Dinitrophenol 14 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water 2,4-Dinitrotoluene 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water 2,6-Dinitrotoluene 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water 2-Chloronaphthalene 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water 2-Chlorophenol 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water 2-Methyl-4,6-dinitrophenol 2.9 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water 2-Nitrophenol 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water 3,3'-Dichlorobenzidine 38 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water 4,4'-DDD 1.4 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water 4,4'-DDE 1.4 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water 4,4'-DDT 1.4 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water 4-Bromophenyl phenyl ether 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water 4-Chloro-3-methylphenol 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water 4-Chlorophenyl phenyl ether 1.9 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water 4-Nitrophenol 14 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Acenaphthene 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Acenaphthylene 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Aldrin 1.4 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water alpha-BHC 1.4 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Anthracene 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Benzidine 96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Benzo(a)anthracene 0.96 ug/L 
17

Appendix 2. Chemical Analyses (continued) 

Date Sampled Field ID Analysis Group Component Result Units 11/28/2005 11:20 Outfall D-001 BNA-Water Benzo(a)pyrene 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Benzo(b)fluoranthene 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Benzo(g,h,i)perylene 0.96 ug/L 

11/28/2005 11:20 Outfall D-001 BNA-Water Benzo(k)fluoranthene 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water beta-BHC 1.4 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Bis(2-chloroethoxy)methane 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Bis(2-chloroethyl)ether 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Bis(2-chloroisopropyl)ether 2.9 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Bis(2-ethylhexyl)phthalate 14 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Butyl benzyl phthalate 4.8 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Chrysene 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water delta-BHC 1.4 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Dibenzo(a,h)anthracene 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Dieldrin 1.4 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Diethyl phthalate 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Dimethyl phthalate 48 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Di-n-butyl phthalate 4.8 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Di-n-octyl phthalate 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Endosulfan I 3.8 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Endosulfan II 3.8 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Endosulfan sulfate 1.4 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Endrin 1.4 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Endrin aldehyde 3.8 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Fluoranthene 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Fluorene 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water gamma-BHC 1.4 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Heptachlor 1.4 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Heptachlor epoxide 1.4 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Hexachlorobenzene 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Hexachlorobutadiene 2.9 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Hexachlorocyclopentadiene 2.9 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Hexachloroethane 2.9 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Indeno(1,2,3-cd)pyrene 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Isophorone 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Naphthalene 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Nitrobenzene 1.9 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water N-Nitrosodimethylamine 1.9 ug/L 
18

Appendix 2. Chemical Analyses (continued) 

Date Sampled Field ID Analysis Group Component Result Units 11/28/2005 11:20 Outfall D-001 BNA-Water N-Nitrosodi-n-propylamine 1.9 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water N-Nitrosodiphenylamine 2.9 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Pentachlorophenol 2.9 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Phenanthrene 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Phenol 0.96 ug/L 11/28/2005 11:20 Outfall D-001 BNA-Water Pyrene 0.96 ug/L 11/28/2005 11:45 Field Blank BNA-Water 1,2,4-Trichlorobenzene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water 1,2-Dichlorobenzene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water 1,3-Dichlorobenzene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water 1,4-Dichlorobenzene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water 2,4,6-Trichlorophenol 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water 2,4-Dichlorophenol 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water 2,4-Dimethylphenol 48 ug/L 11/28/2005 11:45 Field Blank BNA-Water 2,4-Dinitrophenol 14 ug/L 11/28/2005 11:45 Field Blank BNA-Water 2,4-Dinitrotoluene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water 2,6-Dinitrotoluene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water 2-Chloronaphthalene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water 2-Chlorophenol 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water 2-Methyl-4,6-dinitrophenol 2.9 ug/L 11/28/2005 11:45 Field Blank BNA-Water 2-Nitrophenol 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water 3,3'-Dichlorobenzidine 38 ug/L 11/28/2005 11:45 Field Blank BNA-Water 4,4'-DDD 1.4 ug/L 11/28/2005 11:45 Field Blank BNA-Water 4,4'-DDE 1.4 ug/L 11/28/2005 11:45 Field Blank BNA-Water 4,4'-DDT 1.4 ug/L 11/28/2005 11:45 Field Blank BNA-Water 4-Bromophenyl phenyl ether 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water 4-Chloro-3-methylphenol 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water 4-Chlorophenyl phenyl ether 1.9 ug/L 11/28/2005 11:45 Field Blank BNA-Water 4-Nitrophenol 14 ug/L 11/28/2005 11:45 Field Blank BNA-Water Acenaphthene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water Acenaphthylene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water Aldrin 1.4 ug/L 11/28/2005 11:45 Field Blank BNA-Water alpha-BHC 1.4 ug/L 11/28/2005 11:45 Field Blank BNA-Water Anthracene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water Benzidine 95 ug/L 11/28/2005 11:45 Field Blank BNA-Water Benzo(a)anthracene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water Benzo(a)pyrene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water Benzo(b)fluoranthene 0.95 ug/L 
19

Appendix 2. Chemical Analyses (continued) 

Date Sampled Field ID Analysis Group Component Result Units 11/28/2005 11:45 Field Blank BNA-Water Benzo(g,h,i)perylene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water Benzo(k)fluoranthene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water beta-BHC 1.4 ug/L 11/28/2005 11:45 Field Blank BNA-Water Bis(2-chloroethoxy)methane 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water Bis(2-chloroethyl)ether 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water Bis(2-chloroisopropyl)ether 2.9 ug/L 11/28/2005 11:45 Field Blank BNA-Water Bis(2-ethylhexyl)phthalate 14 ug/L 11/28/2005 11:45 Field Blank BNA-Water Butyl benzyl phthalate 4.8 ug/L 11/28/2005 11:45 Field Blank BNA-Water Chrysene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water delta-BHC 1.4 ug/L 11/28/2005 11:45 Field Blank BNA-Water Dibenzo(a,h)anthracene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water Dieldrin 1.4 ug/L 11/28/2005 11:45 Field Blank BNA-Water Diethyl phthalate 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water Dimethyl phthalate 48 ug/L 11/28/2005 11:45 Field Blank BNA-Water Di-n-butyl phthalate 4.8 ug/L 11/28/2005 11:45 Field Blank BNA-Water Di-n-octyl phthalate 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water Endosulfan I 3.8 ug/L 11/28/2005 11:45 Field Blank BNA-Water Endosulfan II 3.8 ug/L 11/28/2005 11:45 Field Blank BNA-Water Endosulfan sulfate 1.4 ug/L 11/28/2005 11:45 Field Blank BNA-Water Endrin 1.4 ug/L 11/28/2005 11:45 Field Blank BNA-Water Endrin aldehyde 3.8 ug/L 11/28/2005 11:45 Field Blank BNA-Water Fluoranthene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water Fluorene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water gamma-BHC 1.4 ug/L 11/28/2005 11:45 Field Blank BNA-Water Heptachlor 1.4 ug/L 11/28/2005 11:45 Field Blank BNA-Water Heptachlor epoxide 1.4 ug/L 11/28/2005 11:45 Field Blank BNA-Water Hexachlorobenzene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water Hexachlorobutadiene 2.9 ug/L 11/28/2005 11:45 Field Blank BNA-Water Hexachlorocyclopentadiene 2.9 ug/L 11/28/2005 11:45 Field Blank BNA-Water Hexachloroethane 2.9 ug/L 11/28/2005 11:45 Field Blank BNA-Water Indeno(1,2,3-cd)pyrene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water Isophorone 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water Naphthalene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water Nitrobenzene 1.9 ug/L 11/28/2005 11:45 Field Blank BNA-Water N-Nitrosodimethylamine 1.9 ug/L 11/28/2005 11:45 Field Blank BNA-Water N-Nitrosodi-n-propylamine 1.9 ug/L 11/28/2005 11:45 Field Blank BNA-Water N-Nitrosodiphenylamine 2.9 ug/L 
20

Appendix 2. Chemical Analyses (continued) 

Date Sampled Field ID Analysis Group Component Result Units 11/28/2005 11:45 Field Blank BNA-Water Pentachlorophenol 2.9 ug/L 11/28/2005 11:45 Field Blank BNA-Water Phenanthrene 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water Phenol 0.95 ug/L 11/28/2005 11:45 Field Blank BNA-Water Pyrene 0.95 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Alachlor 0.6 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Ametryn 0.05 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Atrazine 0.05 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Azinphos Methyl 0.2 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Bromacil 0.2 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Butylate 0.2 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Chlorpyrifos Ethyl 0.05 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Chlorpyrifos Methyl 0.1 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Diazinon 0.05 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Ethion 0.05 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Ethoprop 0.1 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Fenamiphos 0.2 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Fonofos 0.1 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Hexazinone 0.1 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Malathion 0.15 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Metalaxyl 0.25 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Metolachlor 0.5 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Metribuzin 0.1 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Mevinphos 0.2 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Naled 0.8 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Norflurazon 0.15 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Parathion Ethyl 0.15 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Parathion Methyl 0.1 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Phorate 0.05 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Prometryn 0.15 ug/L 11/28/2005 11:20 Outfall D-001 GC-Water Simazine 0.05 ug/L 11/28/2005 11:45 Field Blank GC-Water Alachlor 0.58 ug/L 11/28/2005 11:45 Field Blank GC-Water Ametryn 0.048 ug/L 11/28/2005 11:45 Field Blank GC-Water Atrazine 0.048 ug/L 11/28/2005 11:45 Field Blank GC-Water Azinphos Methyl 0.19 ug/L 11/28/2005 11:45 Field Blank GC-Water Bromacil 0.19 ug/L 11/28/2005 11:45 Field Blank GC-Water Butylate 0.19 ug/L 11/28/2005 11:45 Field Blank GC-Water Chlorpyrifos Ethyl 0.048 ug/L 
21

Appendix 2. Chemical Analyses (continued) 

Date Sampled Field ID Analysis Group Component Result Units 11/28/2005 11:45 Field Blank GC-Water Chlorpyrifos Methyl 0.096 ug/L 11/28/2005 11:45 Field Blank GC-Water Diazinon 0.048 ug/L 11/28/2005 11:45 Field Blank GC-Water Ethion 0.048 ug/L 11/28/2005 11:45 Field Blank GC-Water Ethoprop 0.096 ug/L 11/28/2005 11:45 Field Blank GC-Water Fenamiphos 0.19 ug/L 11/28/2005 11:45 Field Blank GC-Water Fonofos 0.096 ug/L 11/28/2005 11:45 Field Blank GC-Water Hexazinone 0.096 ug/L 11/28/2005 11:45 Field Blank GC-Water Malathion 0.14 ug/L 11/28/2005 11:45 Field Blank GC-Water Metalaxyl 0.24 ug/L 11/28/2005 11:45 Field Blank GC-Water Metolachlor 0.48 ug/L 11/28/2005 11:45 Field Blank GC-Water Metribuzin 0.096 ug/L 11/28/2005 11:45 Field Blank GC-Water Mevinphos 0.19 ug/L 11/28/2005 11:45 Field Blank GC-Water Naled 0.77 ug/L 11/28/2005 11:45 Field Blank GC-Water Norflurazon 0.14 ug/L 11/28/2005 11:45 Field Blank GC-Water Parathion Ethyl 0.14 ug/L 11/28/2005 11:45 Field Blank GC-Water Parathion Methyl 0.096 ug/L 11/28/2005 11:45 Field Blank GC-Water Phorate 0.048 ug/L 11/28/2005 11:45 Field Blank GC-Water Prometryn 0.14 ug/L 11/28/2005 11:45 Field Blank GC-Water Simazine 0.048 ug/L 11/28/2005 11:20 Outfall D-001 Metals-Water Aluminum 26 ug/L 11/28/2005 11:20 Outfall D-001 Metals-Water Arsenic 6.4 ug/L 11/28/2005 11:20 Outfall D-001 Metals-Water Cadmium 0.05 ug/L 11/28/2005 11:20 Outfall D-001 Metals-Water Calcium 57.8 mg/L 11/28/2005 11:20 Outfall D-001 Metals-Water Chromium 1 ug/L 11/28/2005 11:20 Outfall D-001 Metals-Water Copper 4.13 ug/L 11/28/2005 11:20 Outfall D-001 Metals-Water Iron 81 ug/L 11/28/2005 11:20 Outfall D-001 Metals-Water Lead 0.11 ug/L 11/28/2005 11:20 Outfall D-001 Metals-Water Magnesium 4.4 mg/L 11/28/2005 11:20 Outfall D-001 Metals-Water Nickel 1.6 ug/L 11/28/2005 11:20 Outfall D-001 Metals-Water Selenium 1 ug/L 11/28/2005 11:20 Outfall D-001 Metals-Water Silver 0.025 ug/L 11/28/2005 11:20 Outfall D-001 Metals-Water Zinc 4 ug/L 11/28/2005 10:40 Effluent I-002 Metals-Water Arsenic 4 ug/L 11/28/2005 10:40 Effluent I-002 Metals-Water Cadmium 0.25 ug/L 11/28/2005 10:40 Effluent I-002 Metals-Water Calcium 402 mg/L 11/28/2005 10:40 Effluent I-002 Metals-Water Chromium 6 ug/L 11/28/2005 10:40 Effluent I-002 Metals-Water Copper 2.6 ug/L 
22

Appendix 2. Chemical Analyses (continued) 

Date Sampled Field ID Analysis Group Component Result Units 11/28/2005 10:40 Effluent I-002 Metals-Water Iron 1.00E+03 ug/L 11/28/2005 10:40 Effluent I-002 Metals-Water Lead 0.96 ug/L 11/28/2005 10:40 Effluent I-002 Metals-Water Magnesium 13.3 mg/L 11/28/2005 10:40 Effluent I-002 Metals-Water Nickel 4 ug/L 11/28/2005 10:40 Effluent I-002 Metals-Water Selenium 2.5 ug/L 11/28/2005 10:40 Effluent I-002 Metals-Water Silver 0.12 ug/L 11/28/2005 10:40 Effluent I-002 Metals-Water Zinc 96 ug/L 11/28/2005 11:45 Field Blank Metals-Water Aluminum 5 ug/L 11/28/2005 11:45 Field Blank Metals-Water Arsenic 4 ug/L 11/28/2005 11:45 Field Blank Metals-Water Cadmium 0.05 ug/L 11/28/2005 11:45 Field Blank Metals-Water Calcium 0.05 mg/L 11/28/2005 11:45 Field Blank Metals-Water Chromium 1 ug/L 11/28/2005 11:45 Field Blank Metals-Water Copper 0.25 ug/L 11/28/2005 11:45 Field Blank Metals-Water Iron 10 ug/L 11/28/2005 11:45 Field Blank Metals-Water Lead 0.075 ug/L 

11/28/2005 11:45 Field Blank Metals-Water Magnesium 0.01 mg/L 11/28/2005 11:45 Field Blank Metals-Water Nickel 1 ug/L 11/28/2005 11:45 Field Blank Metals-Water Selenium 0.52 ug/L 11/28/2005 11:45 Field Blank Metals-Water Silver 0.025 ug/L 11/28/2005 11:45 Field Blank Metals-Water Zinc 3 ug/L 11/28/2005 10:40 Effluent I-002 Metals-Water Aluminum 28 ug/L 11/28/2005 11:20 Outfall D-001 Nutrients-Liquid Ammonia-N 0.012 mg N/L 11/28/2005 11:20 Outfall D-001 Nutrients-Liquid Kjeldahl Nitrogen 0.62 mg N/L 11/28/2005 11:20 Outfall D-001 Nutrients-Liquid NO2NO3-N 0.015 mg N/L 11/28/2005 11:20 Outfall D-001 Nutrients-Liquid O-Phosphate-P 0.28 mg P/L 11/28/2005 11:20 Outfall D-001 Nutrients-Liquid Total-P 0.35 mg P/L 11/28/2005 11:45 Field Blank Nutrients-Liquid Ammonia-N 0.01 mg N/L 11/28/2005 11:45 Field Blank Nutrients-Liquid Kjeldahl Nitrogen 0.08 mg N/L 11/28/2005 11:45 Field Blank Nutrients-Liquid NO2NO3-N 0.046 mg N/L 11/28/2005 11:45 Field Blank Nutrients-Liquid O-Phosphate-P 0.004 mg P/L 11/28/2005 11:45 Field Blank Nutrients-Liquid Total-P 0.02 mg P/L 
23

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 1 3 9 9 1 2 11 12 0 5 1 1 2 8 18 B 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 1 3 9 9 1 2 11 12 0 5 1 1 2 8 18 X 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 1 3 9 9 1 2 11 12 0 5 1 1 2 8 18 S 19 S 20 2

66

Biological Analyses of Harbor Branch Oceanographic Institute 

effluent sampled on November 28, 2005. NPDES #FL0139912.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

The Bioassay of Harbor Branch Oceanographic Institute effluent 

sampled on November 28, 2005. NPDES #FL0139912.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

The Priority Pollutants Analysis of Harbor Branch Oceanographic 

Institute effluent sampled on November 28, 2005. NPDES 

#FL0139912.