# Patent

## Title
Lactate biosensing strip with two electrodes

## Abstract

We provide a lactate biosensing strip comprising a working electrode and a reference electrode, the two electrodes being deposited on an electrically insulated base support, the working electrode being formed by immobilizing lactate oxidase and an electron mediator on an inorganic graphite matrix and the graphite layer being deposited on a silver layer of the working electrode and the reference electrode being formed by depositing silver chloride on a silver layer of the reference electrode.

## Background
FIELD OF THE INVENTION
The invention relates to a lactate biosensing strip for the measurement of lactate solution. The present invention also relates to a method for the manufacture of a novel lactate biosensing strip and to the use thereof for lactate sensing.
BACKGROUND OF THE INVENTION
Physicians rely on personal examination and clinical laboratory results to determine the presence and concentration of biological analytes in critical care patients. Clinical laboratories offer a wide range of automated systems for high-volume testing and analytical support in a well controlled, high quality environment. However, clinical laboratories can not provide the immediate results needed to properly treat trauma and multi organ dysfunction/failure patients.
To meet the clinical need for immediate test results, several technologies are emerging for testing using reliable, automated analyzers at the patient's bedside including electrochemical biosensors, optical fluorescence sensors, paramagnetic particles for coagulation test systems, and micromachined devices for both chemical and immunochemical testing. These technologies have allowed multi-analyte chemistry panels to be performed rapidly and have addressed previous obstacles such as calibration of test devices.
These tests can be classified as: 1) in vitro, which is performed at the bedside; 2) ex vivo or para vivo, which is performed at wrist-side; and 3) in vivo, which is performed inside the patient. Such tests offer indirect cost efficiencies and savings such as reduced labor costs, decreased blood identification and transport errors, and reduced patient complications.
In vitro or bedside devices are used typically in several departments of the hospital including intensive care units; operating rooms; emergency departments (ER); interventional departments; general patient care departments; and outpatient surgery and ambulatory care units. In vitro diagnostic tests offer a wide range of diagnostic tests, similar to the clinical laboratory. In vitro diagnostic test systems typically are not connected on-line to the patient and require an operator for blood sampling.
Key categories of diagnostic test in the diagnostic market include arterial blood gases, blood chemistries, blood glucose, coagulation, drugs-of-abuse testing, hemoglobin hematocrit, infectious diseases, and therapeutic drug monitoring. Other categories include cancer markers, cardiac markers, cholesterol detection, immunodiagnostics, infectious disease detection, lactate, and thrombolytic monitoring.
Ex vivo diagnostics use external sensors for on-line real-time testing with little to no blood loss. Typically, sampled blood flows through a closed system to minimize blood contact. Ex vivo system minimize problems associated with in vivo sensors, including clotting, inaccuracy, calibration drift, and an inability to recalibrate once in the patient. U.S. Pat. No. 5,505,828 discloses an exemplary ex vivo system.
In vivo diagnostics offer considerable potential in the treatment of most critical and unstable patients. Although many companies are developing in vivo sensors, technical hurdles have thus fair kept in vivo sensors from common commercial use.
Ex vivo and in vivo diagnostics, since they are on-line systems, can reduce quality control and information integration errors that occur with clinical or in vitro tests. Quality control errors are commonly due to operator errors, not instrument errors or device failures. Exemplary errors include inappropriate specimen volume, inaccurate calibration, use of deteriorated test strips, inadequate validation, insufficient instrument maintenance, bad timing of the test procedure, and use of the wrong materials. Clinical information system integration allows test data collected at the bedside to be put directly into the patient record. This improves the efficiency of the patient management process, allowing the integration of the laboratory's information system and clinical information systems, providing a “seamless” flow of all types of patient information.
Lactate is the byproduct of carbohydrate metabolism and product of glycolysis (pyruvate) is converted into lactate under an aerobic condition i.e. deficiency of oxygen in cells. Lactate estimations are therefore important in respiratory disorder, heart ailment, labor diseases etc. The normal concentration of lactate in human blood is in the range of 1.2 to 2.7 mM.
Procedure for lactate determination for example, has employed a variety of chemical and physical technique. Traditional assay involves chemical treatment of lactate in human blood and thereby converting it into colour products which can be measured spectrophotometrically, the methods consists in reacting the blood under test with enzyme namely lactate dehydrogenise (LDH). In such process absorbance at 340 nm is measured due to the NADH formation, it becomes a measurement of lactate originally present in blood.
U.S. Pat. No. 6,117,290 discloses an on-line lactate sensor arrangement. The sensor arrangement includes a lactate sensor, a catheter for withdrawing a test sample, and a first fluid flow line provided fluid communication between the lactate sensor and the catheter. The sensor arrangement also includes a source of sensor calibration and anticoagulant solution, and second fluid flow line providing fluid communication between the source of sensor calibration and anticoagulant solution and the lactate sensor.
In practice there are some difficulties in adopting such a detection procedure for use with blood sample. The disadvantage of such methods, include, lack of specificity, difficulty of standardization, requirement of large amount of blood and use of unstable and corrosive regents. Such methods also involve optical detection and are therefore expensive and time consuming. Additionally, the samples must be prepared. Another disadvantage is that the measurement of lactate level by prior art methods need to be done in laboratory by qualified personnel.
Asha Chaubey et al disclose in Electrochimica Acta Vol 46, 723-729 (2000) the immobilization of lactate dehydrogenase on electrochemically prepared polypyrrole polyvinyl sulphonate composite films. The response time reported is about 40 seconds and a shelf life of about 2 weeks under refrigerated conditions. In another disclosure (Asha Chaubey et al, Analyticla Chimica Acta Vol 49, 98-103, 2000), the immobilization of lactate dehydrogenase on conducting polyaniline films is disclosed. The linearity of response is shown from 0.1 mM to 1 mM lactate concentration with a shelf life of about 3 weeks under refrigerated conditions. It is preferable to obtain sensors with longer shelf life and shorter response time.
Accordingly, it is important to provide a lactate biosensing strip that can overcome the disadvantages of the prior art without losing out on efficiency and accuracy of measurement.
OBJECTS OF THE INVENTION
The main object of this invention is to provide a novel lactate biosensing strip for the measurement of lactate in aqueous medium.
It is another object of the invention to provide a lactate biosensing strip which performs rapidly and accurately the estimation of lactate in an aqueous medium.
It is yet another object of the invention to provide a lactate biosensing strip which is low cost and is capable of being used by even non-medical persons.
A further object of this invention is an assay, which can be performed without the need for elaborate preliminary treatment of blood sample.
Another object of this invention is to provide a lactate biosensing strip, which has a high activity of 75%.
Still another object of this invention is to provide a lactate-sensing strip, which is capable for providing a reading at site.
SUMMARY OF THE INVENTION
Lactate biosensing strips have many advantages over traditional methods, such as fast response, small size convenience, specificity of response, lack of need of any sample preparation, low cost and high sensitivity of measurement. The main advantage of this sensor over the traditional method is sample operation it can be done by ordinary person.
The present invention provides a lactate biosensing strip for use in the assay of lactate in a sample, said sensor comprising a dry strip sensor of an electrically conducting material having at least:

    i. an external surface.
    ii. a screen printed reference electrode and
    iii. a screen-printed working electrode.


Accordingly, the present invention provides a lactate biosensing strip comprising a working electrode and a reference electrode, the said two electrodes being deposited on an electrically insulated base support wherein the working electrode being formed by immobilizing an enzyme lactate oxidase and an electro mediator on an inorganic graphite matrix and the said graphite layer being deposited on a silver layer and the reference electrode being formed by depositing silver chloride on an another silver layer.
In an another embodiment the biosensing strip further comprises

    i. an electrically insulated base support (1),
    ii. a pair of first and second silver layers deposited thereon (2) separated by an appropriate space between the two said layers,
    iii. a pair of graphite layers, each one of said pair of graphite layers being deposited on one respective silver layer and being electrically connected to said respective silver layer (2),
    iv. the first silver layer being covered fully by the respective graphite layer,
    v. the second silver layer being covered partly in the middle thereof with the respective graphite layer after leaving the connecting terminal and working zone area uncovered,
    vi. the uncovered working zone of said second silver electrode layer being deposited with silver chloride (4),
    vii. lactate oxidase being deposited with a mediator on the working zone of graphite layer covering the first silver layer (5),
    viii. the said silver/silver chloride layer forming reference electrode (4) and enzyme with mediator layer forming working electrode (5) being supported on said support (1),
    ix. the working zone of reference electrode (4) and working electrode (5) being covered with a hydrophilic membrane.


In one embodiment of the invention, the electrically insulated base support used is made of polyvinyl chloride.
In one embodiment of the invention the distance between the silver layers is in the range of 0.5 to 1 mm
In another embodiment of the invention the thickness of each silver layer is in the range of 15 to 25 microns.
In another embodiment of the invention, the electron mediator layer comprises a layer of potassium ferricyanide or ferrocene.
In another embodiment of the invention, the hydrophilic membrane is made of nylon or polyester.
In another embodiment of the invention, the working zone are of electrode is a target area used for dispensing the analyte sample
In another embodiment of the invention, the connecting terminal zone area of electrode is an area used for the connectivity of electrode to an electrometer
The lactate biosensing strip of the invention shows an activity of 75% and a response time for lactate detection is in the range of 30 to 40 seconds. The shelf life of the strip of the invention is about 4 months under refrigerated conditions. Under ambient conditions (25 to 30° C.) the shelf life of the biosensing strip is seen to be about 2 months. The strip of the invention is disposable.
The invention also relates to a method for the manufacture of a lactate biosensing strip said strip comprising an electrically insulated base support (1), a pair of isolated first and second silver layers deposited thereon (2), a pair of graphite layers, each one of said pair of graphite layers being deposited on one respective silver layer and electrically connected to said respective silver layer (2), the first silver layer being covered fully by the respective graphite layer, the second silver layer being covered partly in the middle thereof with the respective graphite layer leaving the connecting and working zone area of the said layer uncovered, a Ag/AgCl electrode (4) provided on top of the working area of said second silver electrode layer, lactate oxidase deposited with a mediator on the working area of graphite enzyme with mediator working electrode being supported on said support (1), said process comprising

    (a) depositing a pair of silver layers on an electrically insulated base support by any conventional method;
    (b) depositing a pair of graphite layers on said silver layers by any conventional method, each of said silver layers being deposited with one graphite layer, a first graphite layer completely covering the first silver layer, and the second graphite layer covering the second silver layer only in part of the surface thereof that is away from the surface facing the base support;
    (c) depositing a silver chloride layer on the second silver layer on the part thereof that is not deposited with a graphite layer to obtain a silver/silver chloride electrode;
    (d) adsorbing physically lactate oxidase enzyme with an electron mediator on the first graphite deposited silver layer to obtain a working electrode; and
    (e) applying an outer hydrophilic membrane on the above said first reference electrode and second working electrode to obtain the desired pair of electrodes on an electrically insulated base support in a single assembly.


In one embodiment of the invention the electrically insulated base support comprises of polyvinyl chloride.
In another embodiment of the invention, said silver layer used is applied by the step of screen-printing.
In another embodiment of the invention, said graphite layer used is applied by the step of screen-printing.
In another embodiment of the invention the sample being tested is an aqueous lactate solution or blood sample in an amount of 25 to 30 μL.
In another embodiment of the invention, the electron mediator used is selected from potassium ferricyanide and ferrocene.
In another embodiment of the invention, connecting terminal zone area of electrode is an area used for the connectivity of electrode to an electrometer
In another embodiment of the invention, the hydrophilic membrane is made of nylon or polyester.



BRIEF DESCRIPTION OF THE ACCOMPANYING DRAWINGS
FIG. 1 is a schematic representation of the biosensing strip of the invention.
FIG. 2 shows the calibration curve for the sensor against standard lactate test samples prepared in a laboratory.
FIG. 3 shows the response curve of the lactate biosensing strip of the invention for standard lactate test samples.
FIG. 4 shows the shelf life stability characteristics of the lactate strip of the invention.



DETAILED DESCRIPTION OF THE INVENTION
As shown in FIG. 1, the invention comprises an electrically insulated base support (1) for supporting an electrode assembly (2), (3), (4) and (5). The electrode assembly comprises two electrode systems, a working electrode system (2), (3) and (5) consisting of a silver layer with a graphite layer deposited thereon and an enzyme and mediator layer adsorbed in the inorganic matrix. The other electrode assembly comprises a reference electrode comprising a silver layer partly deposited with a graphite layer and a silver/silver chloride layer thereon. FIG. 1 shows the PVC sheet (i) which comprises the supporting substrate for the electrode. Conducting silver tracking (ii) is the screen-printed conducting graphite layer onto the surface of conducting silver tracking (iii) for the connection of the sensor to read out apparatus. The target area consists of the working electrode (iv) and the reference electrode (v) applies to the end of tracking by screen-printing. An insulated layer is applied over the printed electrode to give them protection; the mass can be coated with one or more legends. The conducting graphite track (ii) does not extend to the complete length of the silver track and the reference electrode.
To achieve calibration of the biosensing strip, the strip was used to detect currents when the lactate solutions were used in concentrations of 1 to 8 mM. The current measured for each of the concentrations was measured and plotted in FIG. 2. In FIG. 3, curve (1) is the response curve for 1 mM lactate solution, curve (2) is for 2 mM solution, curve (3) is for 4 mM solution, curve (4) is for 6 mM solution and curve (5) is for 8 mM solution. This shows that the biosensing strip of the invention can be used to measure lactate in a blood sample if the range lies in the region of 1 to 8 mM in a subject. The sensitivity of the system in terms of the response time to attain a stable current value was determined by analyzing the strip's time variation of current. This comprised initiating current measurement from the time of putting the drop of standard test solution on the strip to the time when the current asymptotically reaches a stable value. It was observed in FIG. 3 that the current attains the stable value in 30 to 40 seconds.
Shelf life characteristics were determined by measuring the current due to a known lactate concentration on strips stored for different periods of time. The data is given in FIG. 4. In FIG. 4, curve (1) is for strips stored under refrigerated conditions (at 4° C.) while curve (2) is for strips stored at 25-30° C.
The invention also provides a process for producing a lactate sensor strip which comprises forming a first and a second electrode on a substrate by applying a layer of silver for each of said electrodes in said electrode, applying a layer of graphite on the handling zone of said second electrode to silver chloride, applying a mediator and enzyme on the graphite layer of the working zone of the first electrode. An outer hydrophilic membrane is applied on the working zone of said first electrode. The silver layers and the graphite layers are preferably applied by the step of screen-printing.
The main feature of this invention is that the sensor is a dry strip sensor. It is found that a similar mix of reagents employed in a wet sensor system did not give a good result across a desired range of detectable lactate concentration.
This invention comprises a substrate for supporting an electrode assembly, said electrode assembly comprises two electrode systems, one working electrode and one reference electrode supported on said substrate and disposed in a spaced relationship to each other. The lactate sensing strip comprises a substrate for supporting a first or working electrode and a second or reference electrode, said electrode disposed in a spaced relationship to each other.
The first electrode is a working electrode and has a terminal extending in to a working zone through a handling zone. The second electrode is a reference electrode and has a terminal extending into a working zone through a handling zone. In both cases, the respective terminals are of a material different from the base conducting layer of said first and second electrodes.
Commercially obtained lactate oxidase is mixed in a phosphate buffer, then proper amount of this solution is injected onto a preprinted working electrode. This solution is allowed to dry at a low temperature, followed by

    i. printing of conducting tracking
    ii. printing of reference electrode
    iii. printing of working electrode
    iv. fixing of membrane onto electrode.


The working and reference electrode each comprise a base conducting layer of silver material along the handling and working zone. A graphite layer is deposited on the silver layer of the working electrode and extends to the terminal; the graphite layer is applied on the handling zone of the reference electrode and extends to the terminal. Ag/AgCl is deposited on the target area of the reference electrode. Working electrode comprises a conducting surface carrying mediator compound and lactate oxidase enzyme. Mediator compound transfers electrons from the enzyme to the electrode, where such catalytic activity takes place. A hydrophilic membrane must be provided on the working zone of said electrode. It appears that the surfactant serves to break up the lipoprotein complex of blood and lactate is then oxidized to the pyruvate by the lactate oxidase. The mediator compound is electrochemically reduced at the electrode producing a current measurable at the electrode, which current is relative to the activity of the lactate oxidize and hence the amount of lactate present in the sample this current is generated through a series of coupled reactions:



  
    
      
        L
        ⁢
        
          -
        
        ⁢
        Lactate
      
      +
      
        
          LOD
          
            
              (
              ox
              )
            
            ⁢
            
              
            
          
        
        ⁢
        
          
        
        ⁢
        ...
        ⁢
        
          
        
        ⁢
        Pyruvate
      
      +
      
        LOD
        
          (
          red
          )
        
      
    
    ⁢
    
      
    
  




  
    
      
        LOD
        
          (
          red
          )
        
      
      +
      
        
          Me
          
            
              (
              ox
              )
            
            ⁢
            
              
            
          
        
        ⁢
        
          
        
        ⁢
        ...
        ⁢
        
          
        
        ⁢
        
          LOD
          
            (
            ox
            )
          
        
      
      +
      
        Me
        
          (
          re
          )
        
      
    
    ⁢
    
      
    
  



The redox mediator is oxidized at the base electrode and the current is proportional to the lactate concertration. The current can be measured by any conventional electronic system.
The following examples are given by the way of illustration and therefore should not constitute to limit the scope of the present invention.
EXAMPLE 1 
Preparation of Graphite Paste with Mediator
100 mg of graphite powder and polyvinyl pyrrolidone (binder) was mixed with 0.01 M potassium ferricyanide (mediator) in ethylene glycol monobutyl ether to prepare a screen printable working electrode graphite paste.
EXAMPLE 2 
Preparation of Dry Strip
Commercially obtained lactate oxidase solution (2 μL) containing 2 U of lactate oxidase was physically adsorbed on the mediator mixed graphite electrode strip and was kept over night to dry at 25° C. The dry strip electrode was covered with a hydrophilic nylon membrane. Before the membrane was applied, it was placed in 10% surfactant (Tween 80) solution in distilled water for some time the dried membrane was then fixed over the strip.
EXAMPLE 3 
Preparation of Lactate Standard Lactate Solutions
Stock lactate solution 10 mM was prepared in 0.1M phosphate buffer. Standard solutions of 2 mM, 4 mM, 6 mM and 8 mM were prepared by diluting the stock solution with phosphate buffer.
EXAMPLE 4
Preparation of Enzyme Stock Solution
15 mg of enzyme lactate oxidase was dissolved in 100 μl of 0.1M phosphate buffer to get the concentration 5U/μ.l to get the working enzyme solution, the stock solution was further diluted to 1U/μl.
EXAMPLE 5 
Immobilization of Enzyme on the Mediator Mixed Graphite Dry Strip
2 μl of enzyme solution containing 2 U of lactate oxidase was physically adsorbed on the mediator mixed graphite electrode strip and was kept over night to dry at 25° C. The said dry strip electrode was covered by a hydrophilic nylon membrane. Before applying the membrane, it was placed in 10% surfactant (Tween 80) solution in distilled water for some time and then dried membrane was fixed over the strip.
EXAMPLE 6
Enzyme Activity
Sigma protocol for activity of lactate oxidase was used to estimate the lactate oxidase activity. The basic principle is that lactate oxidase converts 1-lactate to pyruvate and H2O2. H2O2 is subsequently converted into a colored dye by peroxidase in the presence of 4-amino antipyrine (4AAP) and dimethylaniline(DMA).




In the optimum conditions of temperature=37° C. and pH=6.5, the dye absorbs at 565 nm at the light path of 1 cm.
The activity of the immobilized enzyme was calculated according to the following formula:

U cm−2=AV/εts

Where A is the change in absorbance before and after incubation

V is the total volume (3 ml)
ε is the milimolar extinction coefficient of Quinonediimine dye at 565 nm (35.33)
t is the reaction time (10 min)
s is the surface area of the enzyme electrode
The enzyme activity of immobilized LOD on the working graphite strip was found to be 75%.
EXAMPLE 7 
Amperometric Response Studies
The lactate biosensing strip comprising enzyme (LOD) immobilized on graphite as working electrode and Ag/AgCl reference electrode is connected to the input of the electrometer and was polarized at a bias voltage of 0.4 V for the measurement of the amperometric calibration response to lactate (1-8 mM) (FIG. 2). A maximum current of 60 μA was obtained for the 8 mM lactate solution above which no significant change in current could be observed. The response time for lactate solution (1-8 mM) was found to be 40 seconds for each concentration of lactate (FIG. 3). Results were found to be reproducible to within 5%. The following principle was involved in the amperometric measurements.




ADVANTAGES OF THE INVENTION


    1. The lactate biosensing strip provides a quick estimation of lactate in a sample
    2. the shelf life of the sample is 4 months under refrigerated conditions.
    3. the strip has a linear response in a lactate concentration of 1 to 8 mM.
    4. the strip is disposable without causing any environmental hazard.
    5. the strip is easily used even by people without any formal medical training.

## Claims

1. A biosensing strip comprising
an electrically insulated base support (1),
a pair of first and second silver layers deposited thereon (2) separated by a space between said first and second silver layers,
a pair of first and second graphite layers, said first graphite layer being deposited on and electrically connected to said first silver layer and a second graphite layer being deposited on and electrically connected to said second silver layer,
said first silver layer being covered fully by said first graphite layer,
said second silver layer being covered partly in the middle thereof with said second graphite layer after leaving a connecting terminal and a second working zone area uncovered,
said second working zone of said second silver layer being deposited with silver chloride (4),
lactate oxidase being deposited with an electron mediator on a first working zone of said first graphite layer covering the first silver layer (5),
said silver/silver chloride layer forming reference electrode (4) and lactate oxidase with electron mediator layer forming said first working electrode (5) being supported on said support (1), and
said second working zone of reference electrode (4) and said first working electrode (5) being covered with a hydrophilic membrane.

2. The biosensing strip as claimed in claim 1 wherein the electrically insulated base support is made of polyvinyl chloride.

3. The biosensing strip as claimed in claim 1 wherein the space between the silver layers is from 0.5 to 1 mm in length.

4. The biosensing strip as claimed in claim 1 wherein each silver layer is from 15 to 25 microns in thickness.

5. The biosensing strip as claimed in claim 1 wherein the electron mediator is potassium ferricyanide or ferrocene.

6. The biosensing strip as claimed in claim 1 wherein the hydrophilic membrane is made of nylon or polyester.

7. The biosensing strip as claimed in claim 1 wherein at least the first or second working zone of an electrode is a target area used for dispensing an analyte sample.

8. The biosensing strip as claimed in claim 1 wherein lactate oxidase activity is 70-80%.

9. The biosensing strip as claimed in claim 1 wherein response time for lactate detection is from 30 to 40 seconds.

10. The biosensing strip as claimed in claim 1 wherein amperometric linear response to lactate concentration is of 2-8 mM.

11. The biosensing strip as claimed in claim 1 wherein shelf life of the biosensing strip is about 4 months under refrigerated conditions.

12. The biosensing strip as claimed in claim 1 wherein shelf life of the biosensing strip is about 2 months at a temperature of 20-30° C.

13. The biosensing strip as claimed in claim 1 which is disposable.

14. The biosensing strip as claimed in claim 1 wherein the connecting terminal is an area used for connecting the reference electrode to an electrometer.