llllllllllllllllllllllllllllllllllllllllllllllllllllllllllll • 

OTS0523259 N 

One Source. One Search. One Solution. 

MUTAGENICITY OF TWO LOTS OF FM-100 LOT 53 

AND RESIDUE OF LOT 3322 IN THE ABSENCE AND 

PRESENCE OF METABOLIC ACTIVATION WITH TEST 

DATA AND COVER LETTER 

08 MAR 1990 

U.S. Department of Commerce 

National Technical Information Service 
One Source. One Search. One Solution. 

Providing Permanent, Easy Access 

to U.S. Government Information 

The National Technical Information Service is the 

Nation's largest repository and disseminator of 

government-initiated scientific, technical, engineering, 

and related business information. The NTIS collection 

includes almost 3 million information products in a 

variety of formats: electronic download, online 

access, DVD, CD-ROM, magnetic tape, diskette, 

multimedia, microfiche and paper. 

Search the NTIS Database from 1990 forward 

More than 600,000 government research information products have been added 

to the NTIS collection since 1990. All bibliographic entries for those products are 

searchable on the NTIS Web site at www ntis.gov 

Download Publications (1997 • Present) 

NTIS provides the full text of many reports received since 1997 as downloadable 

PDF files. When an agency stops maintaining a report on its Web site, NTIS still 

offers a downloadable version. There is a fee for each download of most 

publications. 

For rnore information visit our website: 

www.ntis.gov 

/'S':t' U.S. DEPARTMENT OF COMMERCE 

. l!J'- ~ Technology Administration 

\ ·r / National Technical Information Service 

.,... "' Springfield, VA 22161 
CODING FORM FOR SRC INDEXING REVISED 10115166 

OTS0523259 

I'IUTRGENICI"TY, OF !TWO LOTS OF FM-100 LOT 53 AND RESIDUE OF LOT 

3322 IN THE ABSENCE AND PRESENCE OF METABOLIC ACTIVATION 

WITH TEST DATA AND coVER LETTER 

': 

HEXABR~YCLODODECANE (3194-55-6) 
Chem3~al Corporation 

March 8, 1990 

Document Processing Center (TS-790) Room L-100, Office of Toxic substances 

Environmental Protection Agency 401 M Street, SW 

Washington, DC 20460 

Attn: S(d) Health & Safety Reporting (Notification;Reporting) 

Dear Sir: 

Contains Nc CBi, 

'-" .:; 

0 ·' 2 -::;; ::! :;;.; 

w .. ~ _, 

:..~ :~ 

-; 

::.3 

N :;:! 

In response to the TSCA Section S(d) rule published in the December 12, 1989 Federal Register, the enclosed information is being sub- mitted on the following substances: 

2,4,6-Tribromophenol 

Tetrabromophthalic anhydride Hexabromocyclododecane 

1,2-Bis(tribromophenoxy)ethane 

Decabromodipheriyl ether 

Pentabromodiphenyl ether 

octabromodiphenyl ether ~ 

CAS Number 

llB-79-6 

632-79-1 

3194-55-6 

37853-59-1 

1163-19-5 

'32534-81-9 

32536-52-0 

The studies or1 the first four compounds are submitted on behalf of the following manufacturing facility: 

Great Lakes Chemical Corporation Route 7, Box 529 

El Dorado, AR 71730 

T",1e studies on the last three compounds are submitted on behalf Of the following manufacturing facility: 

Great Lakes Chemical Corporation Highway 15 South, P.O.BOX 1878 

El Dorado, AR 71731-1878 

0 

D 
' 

There are no known additives to these compounds. Pentabromodi- phenyl .ether consists of hexa-, penta-, tetra-cbromodiphenyl ether. Octabromodlphenyl ether consists of nona-, octa-, hepta-:, and hexa- bromodiphenyl ether. We are not aware of any ongoing studies with 

any of the listed compounds. If you have any questions concerning this submission, please call me at (317.) 497-6391. 

Sincerely, 

-:u..L~t1£.J/[ j.(</"~ 

Dennis L. McFadden, Ph.D. 

Product Sa:fety Coordinator 

DLM:sb:030!)90 

Enclosures 
TEST DATA 

2.4,6-Tribromophenol 

Acute toxicity studies in rats and rabbits 

Primary skin irritation study in albino rabbits 

Eye irritation study in albino rabbits 

Acute inhalation toxicity in the albino rat 

Acute dermal toxicity in male and female albino rabbits 

Acute oral tcixiei ty (LD50 ) study in albino rats 

Dermal sensitization study in the albino guinea pig 

Bioaccumulation study in fat tissue with 2,4,6-tribromophenol 

48-Hour dynamic aquatic toxicity study with 2,4,6-tribromophenol in Daphnia , Four-day statio aquatic t.oxieity studies with 2, 4, 6-i::.ribromophenol in rainbow trout and bluegills . , · Screening test for microbiological inhibition with 2,4,6-

tribromophenol (' . Acute dust inhalation toxicity study with 2,4,6-ti:"ibromophenol, lot number 3106 in rats 

28-0ay subacute dermal toxicity study with 2,4,6-tribromophenol in albino rabbits' (10/27/77) 

28-Day subacute dermal toxicity study wth 2,4,6-tribromophenol in albino rabbits (12/12/77) 

Pilot teratology study in rats 

Mutagenicity evaluation of 2, 4, 6-tribromophenol, lot number 

3287, in the Ames Salmonella/Mi,crosome Plate Test 

Acute oral toxicity (LD5

.,J study in rats · The bioaceumulation of 2,4,6-tribromophenol in bluegill 

sunfish 

2,4,6-Tribromophenol biodegradation (9/30/75) 

2,4,6-Tribromophenol (TBP) biodegradation (6/i/76) 

Pharmacokinetics study of 2,4,6-tribromophenol in rats 

Photolysis of 2,4,6-tribromophenol 

Partition coefficient of several flame retardants and 

industrial chemicals 

Water solubility of severa1 flame retardants and industrial 

chemicals ...• ·,., .. Photolysis of 2, 4, 6-trib;romophenol in aqueous solution '•:•o 
Tetrabromophthalic Anhydride 

Acute oral study Acute dermal study 

Acute toxicity studies ox tetrabromophthalic anhydride Primary .. skin irritation~ study in albino rabbits 

Eye irritation study in. •albino rabbits 

Acute inhalation toxicity in the albino rat 

Dermal sensitization s:t;udy in the albino guinea pig 

Acute inhalation toxicity in the albino rat after1'pyrolysis 

(3/12/75) 

Acute inhalation toxicity in the albino rat after pyrolysis 

(3/26/75) . 

28-Day dermal toxicity study in rabbits 2l~Day inhalation toxicity in rats 

Hum'an repeated insult patch test with Firemaster PHT4 

Mutagenicity evaluation of 859-74-4 

Pilot teratology study in rats 

The acute toxicity of Firemaster PHT4 to the water flea, 

papl}hia magna Straus 

The acute toxicity of Firema!<ter PHT4, lot nunilier 6332::..8 

to the rainbow trout Salmo gairdneri Richardson 

The acute toxicity of Firemaster PHT4, lot number 6332-B 

to the·blu~gill sunfish Lepomis macrohirus Rafinesque Acute oral toxicity sttluy:' in mice V•, 

Comparative inhalation toxicity tests 

Evaluation of the inhalation toxicity of combt,•,stion prod(icts 

of sel,ected polYJiiers II c 

The bioaccumulation of tetrabromophthalic anhydrid~,in the 

bluegill sunfish ·· . ·· .. , \:,< 

Persistence of PHT-4 in so.il at 1 and 10 !"g/g Photolysis of PHT-4 

Effects of PHT-4 on soil microflora at l and 10 1"9/9 

Pharmacokinetics of PHT-4 in rats ' 

Partition coefficient of several flame retardants and 

industrial chenica!s ' 

Water solubility of several flame retardants,; and industrial 

chemicals ·· ::.:;::, 
ijexabromociyclododecane 

/.Hexabromodyclodcidecane: 28-day feeding trials with rats and 90-day feeding trials with rats ·v,rn vitro 

Geigy microbiological mutgenicity studies of four Ciba- Corporation compounds Pilot cataractogenic study in chicks Cataractogenic study in chicks Mutagenicity evaluation of 421-32B · 

Mutagenicity 

Acute toxicity' studies in rabbits ad rats of two lots of FM-100, lot 53 and residue of lot 3322 in the absence and presence of metabolic activatl.on ·· ·· . The acute toxicity of HBCD lot number 990-17 to. the bluegill sunfish Leoomis macrohirus Rafinesque · Partition coefficier.t of dicarnba, endrin, VEL 3510 and 

Water 

several industrial chemicals and flame retardants solubility of several industrial chemicals, flame retardants.and a herbicide VEL 3510 Hydrolysis of Firemaster 100 HBCD - biodegradabil'ity study 

0 
1,2-Bisftribrornophenoxylethane 

~water solubility of several flame retardants and 

industrial chemicals ~Photolysis of Firemaster 680 

-Pharmcokinetic study of FM-680 

-Rate of hydrolysis studies 

-Partition coefficient of MC-680, chlorendic anhydride 

and chlorendic acid 

-28-Day rat feeding study 

-Acute oral. toxicity study in the albino rat 

-Acute dermal LD50 (1 level) ~Acute inhalation toxicity in the albino rat 

-Acute oral toxicity study in beagle dogs ~cute toxicity (T~ ) study in the bluegill 

-Acute toxicity (LD50 ) study in the rainbow trout 

-Acute heated vapor inhalation toxicity study in rats 

-Modified Draize multiple insult test in male humans 

-28-Day dermal toxicity study in rabbits ~21-nay inhalation toKicity in rats ·· ·~cute inhalation toxicity in the albino rat after 

pyrolysis (ABS resin;FM-680/SbzO)) 

Acute inhalation toxicity in the albLno rat after 

pyrolysis (ABS resinjsb203 ) 

-14-Day range fnding study in rats 

'-.28-Day toxicity study in=-rats 

'Biodegradation study with "c-tagged MC-680 

'-Biodegradability study with "c-:tagged MC-680 

'Primary skin irritation test with mother li~1or 680 . process, code no. 7-29-76-B in albino rabbits 

-skin sensitization test with mother liquor 680 process 

in albino guinea pigs ~0-Day subacute OJ:al toxtcity study with Firemaster 680 

in albino rats (Jan. 6, 1977) ~90-Day subacute oral toxicity study with Firemaster 680 

in albino rats (Mar. 10, 1977) 

'-Mutagenicity evaluation of 859-74-·5 

-Test report (method for testing the 

of chemical substances in fish 

in fish) 

final report 

degree of accumulation 

bod.¥) (Bioaccurnulation 

~Acute inhalation toxicity study in the albino rat after 

pyrolysis (ABS resin/Sb~a,Jcontaining 680) 

Pilot teratology study in rats · '-Teratology study in rats " 

"Tissue residue accumulation/depletion study with Firemaster 

680 in albino rats · 
pecabromodiphenyl ether 

\Acute toxicity.,_,studies., .... in.,.,rats"-a.nd-':ra!Jbits 

\Mutagenicity evaluation of compound 364-5A 

'-28-Day toxicity study in'' rats (6/12/74) 

f· '28-Day toxicity study in rats (6/24/76) ~B-Day toxicity study in rats (3/2/77) "-Mutagenicity evaluation of compound 277-·10 

Pentabromodiphenyl oxide 

., 

ii 

~utagenicity evaluation of compound 345-76A final report. 'The acute oral toxicity of pentabromotl.iphenyl ether to rats • 

\Acute toxicity studies in rats and rabbits 

\28-Day toxicity study in ·rats 

'The bioaccumulation of compound s-512 by carp ~30-Day dietary study in rats with pentabromodiphenyl oxide 

including recovery periods of 6, 12, and 24 weeks 

'-90-Day dietary study in rats with pentabromodiphenyl. oxide {DE-7:1,) 

Octabromodiphenyl oxide 

/j The bioacc~mulation of compound S-511 by carp ~cute toxicity studies in rats and rabbits 

v15Ubacute inhalation toxicity study in rats ~~utagenicity evaluation of compound 345-79A final report j;[n vitro sister chromatid exchange in Chinese hamster ovary cells with octabromodiphenyl ether (DE-79) J,unschedule~ J?NA syt!.thesis assay, compound DE-79 

•28-Day tox~c~ty study in rats 

'13-Week feeding study in rats 

'A range-finding teratology study in rats with DE-79 

" 

' 
,, 

J,.!iuJ.tuaJ, a 1 o . n s·r .l!tz/;aA.LZ.f.Miet,, .91ti: · • " _ . 1610 FRONTAGE ROAD • "onfartt~ ru.;.; oft~~ NORTHsRoox.•LLINOis 6D062 ... EP_,.-oTS 

· ·· li~IIIUitil\111 DODb3bll42T 

, z~~Doocio~b~ 

LOT 53 .Al'ID 

fie x.t 6r-o»u>c)'cf,.d~lec.t£ 

c.;+<:;.q:/;:. 31'11-ST"-..{: '•. 

REPORT TO , 

VELSICOL CHEM!C~L ~OR!?ORATIOi }..!UTAGEl'l'ICITY OF TWO LOTS OF FM-100, 

RESIDUE OF LOT 332:2 L"i THE ABSENCE .AJ."'D PRESENCE 0!' 

METABOLIC ACTIVATION 

P.O. NO. 01515 

AUGUST 26, 1977 

IBT NO. 8536-10908 

" 
,, 

!/ 

.!J~ BfO -TEST ~.ie"-. !Jnc. I! 

·;. 

Dr. D • i\M • WhitaC:::::e 

1810 S:RONTAGE ROAD 

NORTHSROOK, ILLJNO~S 60062 

V elsicol Chemical Corporation ., 

" 

" • ?.::GULA. TORY OIVISI0!'4 

SEP 13 J97( 

R!="C;;:_IVED 

<\ 

' 

3H East Ohio Street 

'chicago. illinois 606U f/ef.A l•r o "<<ocylo.U/et1-r )/e. 

Dear Dr. Whita=e C"-As'\3/7"?-;Si.:. (, 

Re: IBT No. 8536-10908"- Mutagecicit-;;-o£ TwooLots of F~I-100, 

Lot 53 and Residue of Lot 33Z2 i.r'the Absence a."lc! l?::-ese:1ce 

o£ Metabolic Acti\•.:tio::1.- P.O. No 01515 

We are subx::itrlng herewit.'l ou:r re,i.SeC. pages prePared in 0 

co::...~ec:ioil wi_th t.~e above study. 

l' 

A:IP/cl 

.;:. 

' 

/) ~. 

V( p. :.Y'~ A. J. t'!"tsque 

?::-esic!er:.t 

corm <· TIAL 

' 

docur.:ent cnr.t:!= s t: .1:~;:-::.td flltrJK:'l~:-~a u 

w ich is the pr1"lH1'/ . : ttJ··::i t i•::r..-::.1 

.C rporati!ln.ll m!!z.!'!:.: :;::::-:.. .: ~ :·~ ··~ "<t•; r-· . thout r~t:ro::!c~f:cr .. ~;;:...-., '.!t; .~.:.:...~ .. i. •• ·~ ,;..,Ji. 

;ved c: nEJ l!~· .:\:.~,.; .. ~ .. 
1810 FRONTAGE ilOAO 

No~iHaROOK.ILLINOJS 6ooo2 0 

~ugust. 26, 1977 

~!r. D .. H~ ~1itacor 

Velsicol Chemical Co=?c:ation 

341 East Ohio St=eet 

Chicago, Illinois 60611 . . 

Dear Mr. Witacor 

Re: IB'l' No. 8536-10908 - Hu~a<;e::licity of 'I'Wo Lots o:!: E'M-100, Lot 53 anC ResiCue oi Lot 3322 i~ the Abse~ce a~d 

Presence of Met:bolic Ac~ivation - P.O. No 01515 

we are sub~tting he~ewi~h our laboratory 'report prepa=ed 

in connection with the above study. 

AJE'/fd 

Very truly "YOtJ:s, 

/?/lflvt~ ?-~ .. ~ ( ::":isque ?.resiC.ent 

,, 
~ 

'":t~-=··-~~::d a J c -t: 5 r .. ~ I " 

..::=;;,;,.~:k;4 ;•r.::. " 

BE?ORT TO 

v"ELSICOL CE::O.fiCAL CORPORAT!ON 

.l\.fli'TAG2~'ICITY OF T'\'10 LOTS OF FM-100, 

LOT 53 Ac'l!l 3.ESlDu"E OF LOT 3322 n; Ti-::E A5SENCE :-.1>m ??..ES2NCE OF 

:O.!ETABOUC ACTIVATION 

P.O. NO. 01515 

AOGOST 26, 1977 

IBT NO. 8536-10908 

I. btroduc:tion 

~!utagenic: assays we:-e requested fo!" :'M-100 Lot No. 53 (Fi:re!:laster-, white 

powder) and FM-100 Residue Lot No. 3322 (::t=::.a'ble lic;_cid) o::ith and .,;."ithout 

:::letaboiic ac;_, .. :a:ion 2.S !:"a: live:- ::::tic:-osortas. 

The concen.:raticns of the st:.bjec: sa=plas testeC were H-~:.eC. by thei::-

respective salu.bilities b ,;.; ..... e:_~y1sc.Ea=dde~ 

(j 

" 
' I. 

(r 

' 

z 

Tee nr-100 Lot 53 (25 to 250 !-'g/10 Jlil a:oc the FM-100 Resicue, Lot 3322 

(50 to 1000 ~g/10 ~1) in t..~e abse::.ce of rat !.ive::- ciC"osoces we~e :::.ct r::.t::age:".i=. 

Analysis of va.:-ia.."'lce a:'lC =:ultiple co::pa:i.sor:.s of these da~a C.emof..sZated 

that the levels of significa:1ce obtained in the statiStical analyses were 

consistently attribut~ble to nit:"osogua~..i.Cine teSted as the positive control. 

L"l the presence of rat liver mic-osoces. mutants were evi~i!!e::.ced in the 

2-aminofluorene positive cont:;ol for each of the 5 tester- strains. Only 

TA-1535 shOwed I:lt:.tations related li.."le""::-ly to dosage, significant at the 1%0 

level (analysis of va.-i.a...,ce; Appendix, Table lliC) for FM-100, residue 

Lot ~322. That FM-100 Lot 53 evidenced,"~ mutagenic effects in the pr~sence 

of metabolic activation may be related entirely to the concenh"ations of this 

proC.uct assayed. The highest concent::"ation of EM-100 Lot 53 that could be 

dissolved b Ci::::e,;-,ylsclioxicie W2S 250 ll;;/10 Jll. 

The occur:::-e:c.ce ' a£ sig:1llica..'"!t :nu:age:ric e~ects with TA-1535 fo~ FM-100 

Residue Lot 3322 2.!"e .fndicative of base pair substitt:.tions. 

\ 

.Report pre?a=eC. 

Director of 

Data. Ve::i.i'ied by 

Quality Assurance Unit 

Date: 8/16/77 

cl .. 

', . ..:; 
3 

l. :::-::ce:::.:.:::e ?:.:.!:i!is!:eC •. 

adjusted C.ov..-nw·a!"C.. A linear dosage respo::.se is desirable fo:- t..1e Ce!l_'":i:io::. 

of ::lt!tage:ric e!:ects. 

3. Teste:- Sr:-a.:.:::.s: 

Stai::.s TA-98, TA-100, TA-1535, TA-1537 =C: TA-1533 are usecl 

i:c. t.'-J.e p!"esence of :-at live!" :::ieoso=.es. I!l ~e absence cf :::leRbolic ac=. ... ·a.tio::. 

the last strain, TA..;l538, is not included in the assay. Eacl-: test species is 

4:. Co'C.:l-:.s of a positive co::.i::'Ol, a solven: c::::l=.t:-ol a:::.C. S?O::.ta::eous 

:reve::-sion are obtaL'""!eC. fo:- ea:::l:. teste~ s-.::-a!n. 

a. ~!ale :-ats - 200 - 300 gc each (ChZ!'!es !live~ s~~ai:1, Cha.::-les 

B.ive::- 5!"eeC::.g La:,., Inc,) 

b~ Si...'"'lgle L"'l.jection of Aroc:lo:r 124.2 (diluted i:t corn oil to a r:onc. 

of ZOO :::lg/cl) LP. at a rate of 500 ::tg/kg .fiv~ ci.~j:i be!o:-e sac:-:=.ce 

e. D:-~bg water giv·en !£ libit'..t.'"'n & P~i:!a Labor a tor:;.· Chow 

until 12 hou:s beiore sa~.iice. 

d. 0::. fifth C.ay of i::duc!ion, :r-a~ 

head and then decapitated. 

stu."l.ned by a blow to the 

co•l- -::~:r:.,. 

Th dQ:t:rne~t • ... ·• · · • .. 

wh ch is tr.a rop:rtJ' 

Cot. cratinn. It ::t h= ~ec~::. ~!!d :·~: -:;, 

With ut u:pr"d .. t:cn, t:;::n =:t,:. ;;:: 

tn\'cl d ~ ~~~~~ c.;..:.o. 

::.;.;:;;;:1. 

~ 
a. All st.e?S a=~ at 0-4°C cs:i=;; cold, sterile solu::!o=s a:1C. 

;:U.S~aze. 

4. 

b. ,, Livers placed i.J::. beal-ce:::-s con:a:.:l!:lg' 0.15 J..Lg: K:Cl (ap?:-ox. 

1 :::ll/1>"' of wet liver) for weig"-i::q;. 

c:. ititer weisl:i!:.g, llve:!:'s a:e t:-a::.sfe!"!'ed to a bea..~e::- containing 

0.15 !-lg KCJ (3 cl/g::::: wet l:!,ve:-) oi:o.ceci wit.':t sterile scissors, 

and ho:mogeni:eC in a Potter Elvejha.::n apparatus with a 

Teflon ?estle. 

d. Hoo.og=ate c=t:::"-'cgee £::::: 10 :::,utes at 9000 : g (5700 :::?=) 

g. Qcic..1<ly f:-oz= and sto:!:'eci at -BO"C. 

3.. ?'!"e::~a:"ation of S-9 M.;:;t; 

S-9 (0.0'!-0.1 ol) used 50"11=1 

MgClz 8!-!.M \i ""' 

Kc.l ~3 't~-'vf 

Gl:.:cose -6~1:.c~phate 

NADP 4flM 

SaCit= ?hosp!:ate Bt:.:.::e:- :;::E 7. ~ 100!-D-i 

S-9 M!:t is .:. esi::.ly ;:::-e?<!.=~C. e~::h clay, .5.l::er s.:e....-:..l:.:ec!, a=.C. ke?t 

Oil iee be.fo::-e a::.C. C.t:...-:...::.; \:.Se. 

C. Ml.::~::a.~e:esis As saws o::. ?l:a.::es 

Top Aga:::o: 0 ~ 6% Di:::o Agar 

0.5% NaCl 

Au:ocla-.reC. !..::! !.00 :::11 vol::=:tes a:::.C. ke?t c;.t rcoc te::.?e:-a:-...:::-e 

beio:!:'e use. 
-" '·' 

u . 1!inimal-glucose agar mei:!ium in 

Vog~l-:Sor-""J.e:- )..[eCit!::l E 1. 5% Difco Ag~J:. 

2, 0% Glucose 

2. Mutaeenesis Assav P:.-ocedu:re ,·r;rtth s-q ML""<: 

a; 

" 

Acded in order to Z ml !:!Olten top agar at 45 'C 

l. -:-.0,: 1 m:I of overnight nutrient bl"oth cultt.J;e of 

,bactei-i.al tester strain. 

z. Sample _to be tested - lOf!l. ::;;> 

.3. 0.5 ml o£ the S-9 •mix. 

4. S-9 mb: should not be left at 45 'C fo:: more than 

a few seconds . 

b. The contents a!"e mb<ed by rotati."'1g the tube between 

the pabs. 

c. The contents of the tube are then poc:ed on :::li ... ~al 

glucose aga:- plates. 

d. U::ifor:n dist:-!bc.:ion of the top aga~ is ac::Or::plished by 

gently tilt::.!lg and rotating the unco,re'!"eC. plite. and 

e. 

ther:. sat:i.;.g down to ba:den. 

Mbd ... 'P'!g, pou..."'"i!lg, a.""!cl ciistribt:ti..~g should ta.lte 

seconds. 

D. Mu.t:aqer-ic Assav Without Ma:n::nalia.-.i M!ecso::~.es 

1. a. Sa."'Ile as Section C, 1. 

E.. Statistical Co::toutation 

' 

'· 

5 

All data are subjected to analys;s of varia."'1ce and the si'gni.iii:ant ''· ( 

a~:.:e!"e:lcies are fw:.t.."le:- de.:;:1ed by Tukey1s ou!tiple coz:npa:t"iso:t"'..s. Tests 

e.'"(:ceedng t.~e para.r:J.eters a£ spontaneo·u.s reve~sio!'l for eacl; teste st:-air::. a!"e 

reg2.!:'cied. . 

as !:lutZ.ge:lic 

. pro'\'idec! the· counts obtai:led with the test mat2~i~·:i:.is . ~·~· 

signiii~.?·nUy dj~J!'ere.!tt, statisti.t;:a!ly, frO!:J, those 
6 

IV . R.est!l~ 

The data obtained for assays with 2-'"ld without oetabolic actiir:ation are 

, .. 

sl!.!':l:na:i:ized in Tables I and III. The cutat:.on counts obtaineC in the. absence 

d 0 

of rat liver mic:rosomes are given i.."'l Table II; those obtained in t-1-te prese:1ce of 

rat liver r:::ticrosot:leS are tabulated in Table IV. All statistical cocputat:ions 

pe:-fo:r.ned 2.!"'e presente.d in the Appendi::< and data t"elevant to giire!l tests 

(tabie number and teste::- strain) are so-referenced. 
-=--=--~ 

i 

· .. 

Sa..":! ole 

Firemaster 

~ 

TABLE I 

Triplicated Cou::>ts of l.!utager.ic Effects P:ooduced by 

Fi:o=aste:- 100 Lot 53, and FM 100 Residue No. 3.322 

for Fott:!" Genotypes of SaL""nonella. tvchimu::-iUI:'l in 

the Absence of Rat Liver Mic:rosomes 

7 

Triplicated Counts and Computed Ave!'ages 

(Nearest Whole NUI:tbe:-) o£ 4 Test Strains 

Cone. 

(IJ.<:!/10 ul.) TA-98 TA-100 TA-1535: TA-153i 

100 Lot 53 250 16 121 10 6 

15 130 6 3 

15 80 5 6 

Average IT ITii T 5 

100 14. II 106 12 6 

23 128 8 5 

)I 17 116 13 6 

Aveliage iB .117 IT 6 

! 

50 26 98 11 9 

21 119 14 3 

27 117 8 5 

Ave!"age IS m IT 6 

?" _, 20 112 12 ~ 

20 12:1 10 ~ 

13 122 ...2. 5 

Ave!"age 18 118 10 4 

FM 100 Residue No. 3-?? 

~-- 1000 26 128 23 3 

20 162 15 5 

ll 128 20 1 Ave:-age 23 139 19 "' .;::,} 

500 19 114 11i 7 

18 118 13 3 

20 108 10 3 

Ave:·age 19 113 IT .. • 

100 18 113 6 

16 106 5 

19 109 3 

Ave!"age 18 i:ii9 5 

50 25 

13 

14 

Ave!"age: IT 

I 

I 
" 

::.~ 

( 

8 

TABLE I continued 

Tri;:olicated Colll\~S of Mutagenic Effects Produced by 

Fire::1aste:r 100 Lot 53, and FM 100 Residue No. 3322 

for Four Genotypes of Salmonella tvohimurium in 

the Absence of Rat Liver Microsornes 

Triolica.ted Co~i'>.ts and Comouted Averages 

(N~arest Whole N=ber) o£.4 Test Strains 

Colic. 

S ,_,., nle (U<Z/10 pl.) TA-98 TA-100 TA-1535 TA-153i 

MNNG** 10 48 7103 7208 42 

60 7205 5541 25 

55 6956 5549 23 

Average -., 

~- 7088 6099 30 

2 20 2403 2567 10 

29 2891 2669 13 

~ 3083 2478 18 

Average 24 279Z 2571 14 

DMSO*** 20 127 6 7 

21 10& 6 7 

' 18 113 l3 5 

Average 20 liS 8 6 

Spontaneous • ?..eversion ~ 17 134 16 6 

25 101 9 5 

23 .100 _s 7 

Average 22 112 10 6 .. ·-

** ~fNNG: N,...:cethyl-N-r:.i:b:'o-N-nit:-osoguanidine !~at #"08047, AlC=ich Che:::ri.cal Co. 

*** DMSO: Dimethylsulfoxide, Lot illOF27A Matheson, Coleman & Bell Mf~. 

~ .. 
9 

TABLE. II 

Nu:nbe::- of 1luta."lt Color.!.es Computed~ F:-om 

~ Averaged Triplicated Counts of Feu~ Teste:- St:ains 

oi Salmonella h-ohi::::=iu:n Ex;:>osec! tp F1! 100 J::ot 53 and 

~M 100 Residue Lot 3322 i!! Absence of ~ 

Rat Liver !v!ic:-osomes c- .4 ~~:::. ?=- \) l :;-::. .... ~ 

Cone. Counts oi :\·luta..~ts. Each oi 4 Tester 

Sa:nole 

FM lOO Lot 53 

FM 100 Residue 

Lot 3322 

MNNG** 

DMSO*** 

(~g/10 

250 

100 

50 

25 

1000 

500 

100 

50 

10 

2 

ul) TA-98 

N.M. )(*~=~= 

N.M. 

N.M. 

N.M. 

N.M. 

N.M. 

N.M. 

N.M. 

32 

N.M. 

N.M. 

TA-100 TA-153o 

N.M. N.M. 

N.M. N.M. 

N.M. N.M. 

N.M. .N.M. 

. ·~·rc 

N.M. N.M. - N.M. :N.~{. 

N.M.-!" N.M. 

N. ;.,,r. N.M. 

6976 6089 

2680 2561 

N.M. N.M. 

St!";tins 

TA-1537 

N.M. 

N.M:;: 

N.M. 

N.1L 

N.~L 

N.M. 

N.M. 

N.M. 

24 

r~LM. 

·:-;.M. 

c: 

* Cot:lputed as Cp-Cs in w};.J.:::._'-1 Cp is the co~"'lt obtair..ed of a given genotype 

deter:ni::t.ed :for the proC.uct mL"'lus. Cs, the coun:!:: of tl:.e nt:=.ber of reve:-:ants 

occu...~~.g spo:1t.aneous!y. The sig-•:;ca.~ce o.f t..-.,ese d"ffere::.C:es are evalt.:.atet; in 

term of the analysis of va.ria..'"'l.ce a.."'ld m1.:.lo:iple comparisons of the data. 

** MN!i'<G: N-methyl-N-:Utro-N-::-.i::::-osoguaniCine Lot #0804i, A1cirich Che:nl.c:al'Co. 

*** DMSO: Dicet.:"lylsulio:cide, Lot #10F27A Mat..l-teson# Cole::J.an & :Cell M·fg. 

  N .M. == Non-Mutagenic 
. 1x;,·e'':id. B I 0 • T E S T ..f~.dP.k.i.. 211c. lU 

' 

'.~!" ''·· 

{! 

,;:_; .. 

r'~:! .:. ': . 

·~ 

~e: 

':"\ Fi:-er:taste:- 100 

Lot 53 

'TABLE III 

Tri?licated Counts of Mutagenic EffeCts Produced by 

Fi:t"e!:laste:t" 100 Lot 53, and FM 100 Residue No. 3322 

ior Five Ge:1ot;rpes of ~nella tv":)h~mu~iu..~ in 

the P::-esence of Rat Liver Microsomes 

"'· .,, Triplicated Cou.'"'!ts a.."ld .Averagtis·'-Co!:::::n.:teC. to 

~~~ Cone.· Nearest Whole Number EaCh oi 5 Te·~-t· St:'ai:~.S· 

(ue/10 ttl.) TA-98· TA-100 TA-1535 TA-1537 TA-1538 

< 

250 so 114 20 13 19 ~·· 4l 121 20 9 24 

J.:i ill lB. ...2. .ll 

Average 42 123 23 10 21 ... I 

--· 

100 44 127 16 9 29 ~4'0 122 19 8 27 

;u ill l3 ll J£. c 

Average (: 40 lZ1 16 9 30 

50 46 1Z3 17 15 24 

4:4: 120 24 15 27 

48 ...2.2. lJl. ~ 6.2. 

Average 46 113 20 12 27 

25 46 129 30 6 27 

42 119 17 10 21 

£ !.Qi 24 20 ~ Aver2.ge ... - 118 24 12 25 _, 

FM 100 Residue No. 3322 1000 47 172 79 8 19 

43 147 71 15 22 

~ ill. 63 il 25 

Average 43 152 ,, 71 11 22 

500 48 135 43 8 28 

43 121 38 6 26 

£1 ill d" ..2. .2. 22 

.Average 47 133 42 8 25 

100 34 124 21 12 19 

36 116 30~) 10 19 .... 134 . 42 -=2 ..1 18 

Average 39 125 31 10 19 ,, 50 37 126 22 

34 102 

42 116 

Average 38 115 
TABLE ill continued 

'.0 

Triplicated" <;punts of Mutagenic E:iects Produced by 

Fi.:-e:::aster lOG<Lot 53; and FM 100 Residue No, 3322 ~ .... . G \\ ~ c:: 1 11 t..: · • Ior !:: 1ve enor-es o: ... a~::1cne .... a tv:Ja!mu:-lwn 1n 

the Pre~)~nce of Rat Li,ter Mic::-osomes 

ll 

Tri;:llicated Counts and Averages Co:::':luted to 

Cone. Ne~est Whole Nu.a""Dber Each o£ 5 '!'est~ Sb"a.ins 

Sarnnle (g,;/10 ul.) TA-98 TA-100 TA-1535 TA-1537 TA-1538 

2-~-~nofiuorene**  

(/ 

DMSO*** 

Spontaneous 

Reve:-sion 

10 

Average 

"5 

Average 

Average 

AYerage 

2259 

1998 

2076 

2111 

1387 

1275 

1187 

I2"83 

49 

48 

49 

49 

49 

50 

47 

49 

**Aldrich Chemical Co, Lot No, 032547 

1087 39 46 

1039 34 48 

1087 39 46 

107l 37 47 

736 39 3l 

'789 28 39 

854 23 29 

793 30 rr 

133 26 14 

123 17 12 

134 19 14 

Do 21 13 

127 18 17 

129 21 15 

137 15 ..1. ill 18 14 

"** DMSO: Di:nethylsulfo::cic!e, Lot ~10F27A Matheson, Coleman & Bell Mfg, 

") 

2608 

2611 

2661 

2627 

1835 

1800 

1720 

1785 

"' 

33 

24 

28 

28 

25 

20 

33 

26 

"' "" 
."J~ticl ll. I 0 • T E S T .!~.1e4 J.C. 12 

TABLE IV 

Nu=J.ber of Mutant Colonies Coc:::n.:.ted* FrOm Averaged Triplicated. 

Cou...""tts of :E"ive T.ester Strains of ~Salmonella tvchim.\U"it:.r.:l Exp'oseci to 

FM 100 Lot 53 and FM 100 Residue Lot 3322 in 

P:--esence of Rat Liver Microsomes 

Cone. l\:!utation Counts, each of 5 Teste::- St:"ai."':.sSaoole --"'( U"'2'-'l...:l"'O-'ul=·I..) _..::Tc:.A:..-.~9 B _ _;;T:;:.A:..-..::1.::.0 O::__,T"-A:..-...:l;::5.::.3S;:__..::T=A -153 7 T .>. ~ 1538 

FM 100 Lot 53 

FM 100 Residue, 

No. 3322 

2-..A.I:li..'"'l.oiluorene** 

DMSO*** 

250 

100 

50 

25 

1000 

500 

100 

50 

10 

5 

'.·, 

)! 

' 

N .M.**** 

N.M. 

N.M. 

N.M. 

N.M. 

N.M. 

N.M. 

N.M. 

2062 

1234 

N.¥. 

N.M. 

N.M. 

N.M. 

N.M. 

N.M. 

N.M. 

N.M. 

N.M. 

940 

662 

N.M. 

N.M. N.M. N.M. 

N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. N.M. 

53 N.M. N.M. 

24 N.M. N.M. 

13 N.M. N.M. 

6 N.M. N.M. 

19 33 2601 

12 19 li59 

N.M. N.M. N.~L 

* Cocputed as Cp-·Cs in which C- is the count :;:obtainf!cl o£ a given geno-cype 

occu~ 

deter:nined fo!"' the product mi.r...u.s Cs, the coun.t oi the number of revertants· 

.... ,.g spon't2-~eollsly ~ The sig::i:ica.."'lce of these ,.::H.:e.:-ences are· evaluated in 

te..-m of the 2-'"lalysis of \'"2.:"ia..'"'lce and multiple ccmp2.:."isor...s oi the data.. 

** Aldrich Che=ical Co., Lot No. 03Z547 

*x** 

**"' DMSO: 

N 

Dbet.,.ylsulio:cide, Lot f,!lOF27A Matheson, Colema., & Bell Mig, 

.M. = Non-Mutagecic · 
·, 

'. 

l3 

" 

'· 

Fif 

~== 

•' 

" 

'· 

0 

,-.. " _,_,,._ 
. ' , . 

TA-98 IN A.3SE:-lGE OF" M!:7.-\30!..!C ACT:V AT!ON 

Sl!·Tl."irlC-'L Str:•.~o\·;.'i 

·--~ ------------------... --.......................... -.... ---------...------~---------

bP· IQ.t_ldl .::»l " .• !JAh;o 

:~1-:;. ~. UI-'H:rl!.~; ... :.ioll .!"t:t. f':J'o io<IJro; .. r:; L:~\J~·: .~ .·, A.1 ~•·:· 

·------------~----------------------------------~---~-----------~ 

.~,~·H•I l 0 J H.J3J3 c.02i7 .H,~JJJJ\ J.4.,6l ~I! .rJUU1J :.u ~ L'l:•; u 

;., :·.i: G/ :C. J, !-t.vuOO 4.502A L! .iJ•JuUO j.., 0-4 !U'I :O:tJoiiOUU 21:r.uUUll 

i:·f·'~(l .3 1

1.01)1':7 1.5275 ~~JJJJ.; v.~·ilY 1~.ooou 11 .. CIIUII 

.,:- J !t.ooft7 4.11"33 t7 .. JJ.IJ41. L. '-fll7 l7.Vt1UIJ ~:l .. 1lt:IJI! 

fJf.l:./~!:10 3 !l .. JJ.U 0.'>773 IJ.JlJJJ O,JJJl t:, .'lviiO l ttoUt..liU :-''lr-1-JlUU 3 -"'·0•)00 4.Sii2o "..! l .. •JuuuiJ ]. • n·• !;),. l4- .. 1Jt)U\J <!";: .. t•~t.U t'rr.::ISU J 14 .. bnn7 3.2tol6 t(,; .... d.U"I 1. nSSY 21 .uuuc.. 27 .,utll.llJ nr.r:12= 3 17 .. no~:~7 .,.0415 to.3J3JJ :t.JJJj \J .. ooutl :tu.,UU11U 

f~lUO/lUCrU :J '2:.! .. coo7 l.OSSL 'J.JJJJ4 1 .. 71.31:! :lii.Vfitn,; '2c~uuoo 1:/'IJVO/SUU 3 19,.0000 t.oooo 1.uuouo ~.!)774 li:J.OOOO 2U.vOuU Fl"·lOO/luO 3 P .btul7 1.5275 2 .. 3JJ3l U .. 1Hll9 lo.U\J6U 19 .. UUIJD 

1"")"'10U/~U J 17 .:.!J 33 6 .. ~sa3 .;4 • .!J3J3 J .. lja..t:z lJ.OftOU 2~ .. uuco 

----------------------------------------------------~--~----------------~------------

.................... , ........ ~------------ ... -----------~-------------.---------------------------- ... ~-----

:5t1UKC!:. Oti 

v;r J..\!lUI• 

De.t:M!:i::S 

sur- ur '" . s~~ll-""'es r;:=.::olHi ~~;.r.. 

!:i!HJ-'kE 

t.C::'J !:::L 

ClllilCAL or 

r-·HLut: STGO'jlf"'lCO:,'•t::: 

----~---~--~-·------~---~-----~----~·----------------~~--~-------~--------------·---~-

• 35 5 a (I Ott!:>., U4 ll 2l •. JJ .01 

2. 

--------------~-----------~-----------7·------~·------·----~-------------------------- l'OI,\L .J~:.!2UOtot: ... u4 35 

---~------------------~--------ft·-~----··---------.. --~--------~···~---·-~.---------------

,;;:::; 

-----~--------·-------~--·----- ............................................. --------------·----... --............................ ___ .,. 

Cll~ ~o~,jh P'll}ol!.i L; r lUt·S CtJ;P1CII ?GPUt . .\T !lH•.!:i 

T~~~A'I!"EI'l1' ft ~!::.U• CS ~::;c:.r.,t L~IJC:i:) ll P!::kCE.!~T L:E"t~!.l -------------·--------------------.. ·---··-----7-.. --................. _____ ~···----·---·------;..::,._~-~----

rt~~:/2Su t:;,.JJ.!J X 

F':'•t CIU/~U !7.3Jll X 

F'"'tUU/100 17 .. r.on7 l 

fo'lFI::./2!5 17 .Dnb7 X 

~-''11- ::ttliO t k .fiiHJIJ X 

:"'!-'lUO/!it;O 1'J.1JUIJI) 

Ot~til• tlj.:)no7 ' 

X 

;;;. :ll.oor;,7 < f~~lU0/10uO 2~-"'llh7 x. 

,.lroi>G/2 :io:•.UVlru l 

i-" Ii- i:./!1.1~ (.o.nM•7 :< ~ .. ~ .. n~; 

;::;:.·.";."~-~' - ..... : .. ., 

·' ·.•;. 
' 

T~-\.3!Z !3 {RE!''E:?..:ENCE 7A3U n 

TA ... lOO IN .~SE:-J'C:E: OF ~t:::':'A.30UC ACTI"v~~T'fnN' 

St~TISTlC~L Su~~~HY 

_ _.. _____________ _.. .................. ________ "''"'"'"'"''"'"'"'""-----..... ·--~--,..,-·------------------------ ............... STMIOA.I(O S'i'A:.OA.RU 

r .. t:ATI'II::t•T ·" nc;.u, DC: 'it H 101-1 V.:..tH.C.~;Ct: !!P.P.tJP ,. I~ll:-!t.ll-1 AAl [~IU!Io! 

......................................... ----------··---.... ------------------------------------------~-----------

'-!i\l'tG/lU J 7UHif .. uUIJO " 125.1759 !.Soo~.uOOuil 72 .. 2703 ~qsc .. JcJoo 721)5,.COilO 

kNNG/2 J ~797l .. J33J 35u .. ::.72Y 12:t:i0l.J7!:1tJU 202w~UJ4 24;tJJ.OtlOO Jlt!sl.UOUO 

!HISO J ll~.JlJJ 11J.59:Z7 u ..... HJ:0.:5 0 .l 73-. tvO,.IJ(iUU t27.::oco 

SH ' l!l.oon7 li.3477 JH.JJJ:t.!:l l1.17C..O hJO u00'il.l 1J4,.:.tOCI) 

F lilt:F.!SO J llll •. HJJ 2il~:i~21 710 .. JJJlS tS .. H7a J30.tl0UO uu.oooo l".thE./100 3 11 D. oC.oi 11.01~L nl.lJJ:z~ o.Js~., IIJI).OOOO ll!:!.•JOOO 

Ftlol::/:30 J tl!.JJJJ 11.591]2 134.JJl25 tJ.,o918 ':HI .OUUO U9.000U 

Fbt./25 3 11 a • .!333 5 .. 5076 JO,.)JJ25 Lt79!! 11:'2:.(/QUU 121.0\lOCi 

r·HtODJlOOo 3 139.3333 1~.6299 JBS .. J:JJ2!:1 u .. BJJ ll!!.UOOO 1tl2 .. 0000 

flHOO/SUO 3 113.3333 ~.0332 25.33325 2.9059 101:1.0000 lll:I.OOOU 

Flol100/100 J 109~3333 3 .. 5119 12. 33J2!) ~ .. 027b 106 .. 000\) UJ.OOOO 

f!-'100/SO 3 UO .. oo67 .... 1258 2Z .. 3ll2S 2.128~ 1u1.oooo uo.ooao ............................... -... -..................... _ .. __ _.. .................... --....... _ .... _________ ,. _________________________ _ 

c 

.\'i:.LYSIS or· V.U~Ut1CE 

------------------------------------.. ------------------------------------------------ t.::n:I.. 

SUURCS: Of ::a!TIC.U.. OlVARl~TION SU~ Of SCUAk£S 

OE:G~.EES 

OF 

ffi.E'£00/11 

v:.nv.~c:: 

ki.TIO f•I,U.Ut: SIG:HP'!C~:.CE: 

iHTHih .21HOOOOE+06 

11 

24 

.,13U9b53E+OB 11!.0.50 3.10 .01 

----·------------------------·-------... -------·-------------· .. --·------------------------ TO"tAL. .14"\342BE+U9 3S 

TU~t:·t Is !1UL!lPt.l: C!JHPAHISO!i 

---------------.. -<L•O.----.. ·---... -..................... ~----------·------------··----------------------- .. C0•10N ~O~ULAT!ONS COH~ON POPUL~!!O~S 

_________________ Tf!'E:.\TJoo!::r.T t' J.I.£At& (5 PERCEN1.' .L.Ellt;LJ (1 P!::HCE!iT L!::Y!;:!.) .. _______ ................................................................................. __ .,..,_., _____ ;"' ____________ .. ,., 

1-",..100jl0U 3 109.JJJ3 X . X 

flfii/2.:,0 3 llO .. JJJJ X , X 

FM10U/50 3 110.0ou7 X X E"IH~/50 3 ,t1l ~3333 X X 

SH 3 1ll.bbn7 X X f"~l00/!100 3 llJ.Jlll X X 

01-'SU J 115.JJJJ X X 

S"I:?E/100 3 ll6.0on7 X X fl.H~/25 3 116.3333 X X 

n·too,tooo J 1J'J.33Jl X -·:- =::.\ 

:-!:r.&NG/2 3 27~'Z.JJJJ X- • -- •.• ':._ 1. •. 

f'!~fiG/10 J ?o~a.uooo . ( '• .:,..,;!\ :• --:~· .. 

. ,,,::·· · .. .,-,_) 
, 

,, . 

TA-1535 !N A.BS:S:-JC;:: OE' ~f.Z'!'}..BO:W:C ACTI'\"A'!'!ON : . ,, 

s~r,TlSTlCAL SUMH;.Hy 

·-·----·--~-----------------------------------·---------------~---·-----------~-~----- ST!WOAPO s'f;. .. o.:.ku titt::A'I:~t:.:ror " !1t:.U.s DEv(~TJ.Ot; V.\Hl:.hCt-: f:..ttPIJH tiW[~IJ~ 1AXl•"-lt~ 

--.. --.R>-----~----- ... -----~·-------------------~----------------------~------------- ..... --- ~t•r·G/11) 3 QO'J'\t,.3J33 9b0.1419 92ld12~~U011l) !:l&o\.HI32 ~!)~l.UUUO Hu!t.UllOO 

:.w~<r;/'2 3 2571.3333 95 .. 57)9 fJ 1H • 37SDO ss. 179h 247U.OOOO 2Do'J,.OOOU 

0"'~(1 3 b .. 3333 4.0-=-15 tb.)JJ33 2.33Jl n .. uuuo U .. lHIIJO ., 3 10.(!000 !;.5618 Jl.UUQOU l. 2140 ~~ .. u•;uu Lc.uouu 

!o"li<I-U'2SU l 7. 001)0 :z.&..;sa 1.tiVOVO 1~5275 S.UiHIO lll.UOVO 

F'lHf./100 3 u.aooo 2.6~5~ 7~1iUU00 t.S27S, a.ooUo 13 ,.Ul/00 

F l.t<C:/SV 3 1.1.000(1 3.0000 9.uuuou 1n7Jll a.uooo l q .uooo 

FlF!::/25 J 11l.J3.U la527'5 2.llllJ O.dtH9 9.oauo l:Z.UC.\lU 

f'!ilUO/lOUQ l 19 .. 3333 4 .. 0415 1o.33JJ4 2.3333 1.!:1 .. 0000 23.0000 

f~100/t!UO l t3.bOb7 4 .. 0415 l6.J.)JJl 2..3333 10 .. 0000 u .. oouo 

rk11JOttou 3 14.\)000 0 .. 928:2 41i.OCJOUO ;,.oooo 1o.oouu .. z .. oooo 

fHlOO/SO 3 10.bt:lb7 1.5275 2 .lJJl3 0.HiH9 9 .. 0000 12 .. 0000 

_ .... ___________________________ .. ___________ ....................................... ________ .. .,. __ ......................... -.. 

AN.:tLYSlS Ofo" 'IA~l:.r;c:; 

' Lt:..,I:::L 

SUUhC::: Uf C!<ITICA£. or· V~kl~tlUN SUM OF SUUAHES 

DE:GH~ES 

'OF 

Fl<!::E!JO!i ?"•'.i.\LUE Slt:ii'41'r'lCA~C;: ------................................................................... _ .. __ .......................................................... ___ .. _________ ..... __ ... __ ... 

.. 112Hb7.E:+09 

i1 ['t!11N 

11 

.. ~101951t~E:-08 tll.39 3.10 

. ' 

---------------------------------··---------------·~------------... --------------... ------- TOTAL. 35 

--... ---··--------·------------.. ---':"'-------------------· 'l'UiC.W' 'S ~ULtii"L.E:. COMJ?..\fllSUtl ----------------~-----

-------~·W---~-------~----------Jr-~~~~~~-;~~~~;;~~:~---------~~;~:~~-;6;~~;;~~~;----

:n:t:;."Il'!Eln· li MC::Art .,-,-,:;!}~.·· (5 ?ERC:.1'11 LE'IELl 0 PE:~CSNT Ll::ii'!::L) -----"'---·~-----~------------------------------ ............................ _____ ............ _______________ _ 

flf!C::/250 

OJJSU 

so 

nrt£J25 

f'HtOC/50 

f'IFI::/100 

flPE/~U 

trU00/!)00 

F~tOii/lUO 

n"toot1ooo 

11NtoG/2 

·"'I'ING/11) 

3 7.uooo x x 

3 8.,3333 X X 

3 10 .. 0000 X X 

3 10 .. 3333 X t. 

3 10.bb07 X X. 

3 .11 ~OCIOO X X. • • • • • • : ,: ... t ; ~~· J ll.OOOU X :C • • • """i\P';:;o,.'tr .. ~., \ ·,a'~\t:.~··· 3 ll · "1 X v ,...:t':\t~· ~\ .... - • -~,.., ... • .. aoo ... ·llrlOI:·l' .. • .... ctl.,;t.;:: .. l• ·: ('. ~ 

3 t4.oooo X X ... .!.t ~e::n\:;.:•c: •• -.f -.;~·,..,,~·'"-:-':';. 

J 19~333.3 X .: .... ,~1:. S~\:t:\'.!:• .... .., .. :;,t,\'1 ... .,, ..... i ,:.- 3 i!SH Jl33 X ,t\·~ .;.. \'~'1 \UW" .... .. 

. .. ·:.~·-c'i\·1.. lo .:. .. •• ua ~'! .•. \•·:~ : : ·, ... ~ •'!~ 3 o099.JJJJ • • X • ... ~ ._ • • _-..:o~l\ ... • "\l ,.,~;o ~ _ r.• , .•• •. 

. . : ." Cili~il r: ~~,;~~t\io·, .:~:::· ;~ ..... ~:~\· .: 

------------~---··---------------------- .. · ... ·------------------- · \'1\~. ·,.·. ~-~--------------- ·cl ~::';.''o;' :""" · ,, · \a ,a •. , . -- 1_. 
17 

~·rcJ,_.,::i.:!. B I 0 - T: S T .!~ J,-...c. 

S'U'HSHC~L SU11ft.\R't 

---~------------------------------ ............................................................................................. _ ... _" ____ ............. _____ SV.NiJ.olfl:O S"r.;.I<Oo\KU 

Ti"f.:,\Hit.li't • P:OAN U~li IAllO-" V~:tU~CE: l:.h l"'i!k ;,!,.l!~U:o- ~.:..ll,<tU;.. 

-----·-~---... -------~-----... ----... ---... ~------~------------'--·--------------------------------

;..Nt.Gt 10 3 '30.UOCIO 10.4-103 ]U'J.UOUO(J 0.0271 2J. .. OIJG0 "2 .. 0000 ~~)..!(:./2 3 13 .. b6n7 .; .O·l1!i l&.JJJJJ 2 •. BB 1U.OUU\J li:l .. uuuu DI-ISU 3 b .. 3333 1 .. 1547 1 .. 3JJJJ ll.o.;o7 s .. ouou J.ouvu 

•• 3 b.,UUUU 1.0000 t.ouuoo u.~no1 S .. OUOtl ? .. uUtlU 

i'"1Pt.125U l ~.uUtlO 1 .. 7321 .l.ulllfiJI) L.,OUO(I 3 .. 0001} 6.UOQIJ 

flP~/100 l ~ .. o0o7 0~5774 CI~Jll.U 0.3JJ3 !l.Uuoo C.llOOO 

F'UH .. /51.3 3 ~ .. b6ti1 J.OSSl 9.))3:53 1.71:1J8 ~ .. OOOU 'J .. \lOOO 

F"liiE:/'2~ 3 4 .. 3333 o.~;;.; o:JJJJJ a.JJJJ -t .. uooo s .. ooua 

fiH00/1000 3 3 .. ba07 1.1547 !.JJUl o.ooo7 3 .. 0000 ~.ooao 

Ff.'tOU/500 3 4 • .3JJ3 2.3094 S.Jl3J3 L. 333J 3.uooo 7.oooo 

F'!HOO/LUO l 4,.Qt:.b7 1.s:ns 2.Jllll o.aat9 .3.0000 b ... oooo 

fHlOU/SO 3 2.6667 o.Sii4 (I.JJl)J U .. 33Jl 2.000i) J .. uooo 

........................................ _______ .. _____ .., ___ .., ____ .,. _____ ... _..__~_ ........................................... _______ ., ___ _ 

SOURC~ OF 

'1.\R t: . ."~ !Off 

DEGi1£ES 

Of 

SUM CF--SCUAA.ES FRr:E:OO~ 

V.\RUNCE 

lUTIU 

LI::VEL 

CHIT!CAL UF' 

__________ ... __________ f~'f"ALUE: ~1G~1FlCMIC:: ..................................................................................... _ ....................................... ________ ~----

.. 1tUJOOCOE+04 II .170909H:: .... oJ 

.Joooooo~-u~ l4 .t25oooot. .... oz 

----·--·---------------------... --··----------·~··------------------- ... --------------... --.. -- TOTAL .21t!OOUUE..+U4 35 .., .. .., .. _________ -:.,.•~-----~-"'"'_..':~"::.."':'.":.''::':':".'":.."::::-:::::-:~--::.:;-_---·-:--w'""'""-'"'"'-""""'"'"'"" .. .., .......... .,. .. .,. .. .,. .......... .. 

l'UJtE:'t 'S ""'JLT!Pt.E CO!>'?Alo': [SOh 

----------------·-------------------------------------------------------~---~------- COM'41'JN POl-'UL: • .':'!ONS CUI>llo!.U!l F<OPUL~T l.UNS 

T~£.\T~E~T H ~EAN l5 PERCENT LEV~Ll (1 pgRc~~T L£V~Ll 

-----------------------.. ---~-----·~---------------- ............. -------:-·--·-------------.......... .. 
18 

T.A.-98 IN ?:t.=:S2NC'E OF' ME:'A.E:OL!C ACTIVATION 

~r~rtSTlC~L SU~~AkY 

----~---- .... ··-----------------.. ------------------------"'------·-·-..................... ~--- ... -------

" 

2-Af-IHID/10 3 2-Al'ILI~u/:5 3 

OMSU 3 

s~ 3 

F'IKE:/2~0 J 

nRl:;ttoo 3 

flf'E..I!:zU 3 

F'IHF.:/25 3 

io"kl00/100(! 3 

F/1100/500 3 

f"fo<l00/100 3 

F'MlOO.f,SO J 

21ll .. IJOUO 

Htll.UOUU 

4ll.b!lo7 

4ts .aao7 

42 .. 1}00\J 

4ll .. J3JJ 

46 .. 01JOU 

'iS.oooo 

.'iJ.OOUCI 

4b~bo67 

3a.oo.o7 

31 ,.60&7 

Sl'A'olll:.RO 

DC:Vt.l't'(U~ 

1J3.9'139 

100 .ll97 

U~S774 

1 .. s:ns 7 ,.54!:Hi 

3 .. 5119 

2.0000 

2.6458 

<\ .. OOOU 

3. 2146 b.~291 

4.0-415 

ST A!WAP.O 

I::]H~UN r"I/'II!'!Uk 

17949 .. 0UUOU 71.H9'i l'JIJij .. OOOO 22S9.UOOD 

l UU4ri. oOOtlil SLlOl~ 11'1:11.\lU\lU Ll~t7.0UCJO 

u.JJJJ-+ O .. lJJJ 4!1.0000 49. 0\Jt)U 

.l.JJJJ.; o.<iat'i 47 .oouo so.uouo !:Ji.OOUUO 4.,Jsa9 3!1.00\JO so .. uuoo 12.JJJ34 2.027b 37.0000 4~ .. 0000 

4.00000 1 .. 1547 44.,.0000 4!1 .. 0000 

7.00000 1.5'275 4:l.OU\l0 47.0000 

ltt.ouuuo 2.3094 39.0000 ol7 .. 0UOO 

1U,.J3Jl; L.ll559 43.0000 49.0000 

41 .. 33334 J.?tlii H.oooo 4ts.t10UU 

lts.J.lJl.t 2.3333 34 .. 0000 42 .. 0000 

------~-~----------------~---------------~~~-----n-------~---------------------~~----

,\N"AI.1S1S Of VARIAHC!:: 

-----------··--------......... _ ........ -~---------------------... --............................ ,..,.. ............. ~ ... -----.... -·-- D~G~£~5 LEVEL 

5CIURCE l!F \\ '· OF !-'E:Ah ""ARIANCE Ck!tlCAL Of 

VARlATl(!f'i \S.~-~ .•. or ~CIU;.R~S f!t:::::oo!-t SULI.\~£ RA':IO F-YALUE StGN!FlC.:.r.C!;: 

.................. -.. ----------·----------... --------·---.. -------·--'n·W~-----------------------------

E!ET~EF.:ll 

HI!HlN 

.. l469bi3tsK+I18 

.Sh3:2H13E•05 

11 

24 

.1330U3:2E'•07 

.2347005~+04 

Sb9 .. 25 3.10 .. 0 t· 

_______________________ ................. _ .. -~------ ----------------------......... ---------------~-

•••-••••-••••••••••••--•••-•••---••••-v--•~--~--··-•~----------~--------------~---~--- CO~~ON PCPUL~710h~ 

(5 O:SitCE:•'O L::'iE:L) 

COHHDh POPULATIONS 

(1 ?~HC~N! L~VEL) 

·----------·-------------------~---~-----------~·---------------·----------... -----.. -----
TA~IOO OJ 'P!'.::E:S:E:N~ OF'_~t:::-:'A30I..!C Ac::'!YA":'ION 

STAT!S~z'C;.L :)UHf4AtiY 

19. 

_____________ ..., _________ ..,,.,__,. .... ..,., .. .,..., .. .,. ............. - .. _ .. ..,_ .... ..,,u .... oo ..... .,._..,~------------------------

SlANO.\kO T!tE).T.'"lf.:NT 

SrA:IO.:.RO 

N HKArt DE:\t t.\Tit.Jtl V.\Rl;lkCi:: E~iltlR ~ {ll (I"'IJM t'A4I.~U~ 

........ ~-------------------·------.... ;..;.. .............. -............ _ .. ____ .,. ................................. -............................. ___ .., ___ 2· .. 1\.:~ LJoO/ L 0 ] 1071.00(11.) 27.7129 7?6.\lOOIJO tbqonoo 1UJ~.oooo 10li7 .01)1)0 

01'\.S(l 

:2-A.'*J J.L/S 

) 

] ~7!i:J.UIJ~U130.0000 

5 1

L101& 34':1J .lltl1.ziJIJ 3~.1::!23 73b.UO~O a:~.;.ouuu 

6.01J'29 37.001'100 3.5119 12J.0000 

S? ] 

lJ-&.0000 

t3LuOOO !::1.2915 2B .. Qt)UUO 3.05'51 l:t7.110li0 ll7. •J/)UU 

~·rt.z:.r2!iO 3 UJ·.oouo hl ... t4!l9 lO).O(lUUO Su~5S!l lH~UOOtl IH.oouu i'"lJ{E:IIUO J 12U.tJ!lt:t7 7 .1)':,346 so •. n J2:, _.~O'J"l llJ.OOUU lli.OOOO 

i"'lHI:U!)O 

FlH!/~~ l 

J 

117 

113.0000 14 .. 79A& 219.-ooouo R .S \-IU ~o.OilOU 12J.OOOIJ 

.ob07 12.0554 

Fl'lltJO/lUUO J 1~l.J3Jl 

l"t5.JJ.J25 a.9f.I02 lU~.Ol,IUf) 129 .. 0000 

17.&lb3 lLO.JJ35.0 

f~lCI0/500 3 1.33.0000 

ll.i .. t70ll lJa.oouo 112 .oooo . 

F!'-1100/lCO J 12-7.66~7 

11.1355 124.000UO 0.,4291 1'2.1 .. 0CJVO HJ.UOQU 

FHL00/50 J 114!.6607 

9 .. 0tb5 B1.3J32S S.,2D6a uo.oooo 134 .. 0000 

12.0554 H:;, .. JJJ25 b.9oii!ll 102.0000 12a .. oooo 

____ .................... -------~-··--···------------~--~-··--------------------------------·---

AN~~YS!S or VAN!~NC~ 

-----------------.. ----~-------------------.. ----------.. ------------------------------

SOUR.C:!:: OF" 

VA~IAtltl!ll SUH Uf SOUAHES 

D!!.Grtr::::::s 

or Frt!:::::oo~ 

Y.\iHA«CS: 

R.l.1'IO 

CP.i':'!C.\L 

f-V~LUI:: 

.LEVI::L 

---------------------------·-------------------·---------------------------------------- SIC~H"lC~NC5: "' 

.33&7Q75E+07 !I 

.UUOS31E-t-ll5 

.3061523£•Ub 

• .Jsa7'2.l4E ... oJ 

.01 

_________ .._ ____ .. _____ _. ............................................................................ _______ .. _____ .... _ .. ____________ _ 35 

=--~·------:------------ .. -..... ··--::-·-:~-=-:~~=:-.:-:--:::--:::-.:---,-:--::.-:-""·----:""·-------------~------

TUKE~·s ~ULTLPL~ CU~PAMtSUh 

---·~ ... --~--- ... ------------------.. ·-----------------------------------·--... ----------------- N MEAN CO~~ON PUPUL~110NS 

(5 F!~CF.hT L~V~LJ 

COMMON· f<'UPUL.o\riObS 

-----··-··-----,u••-•-••- t1 ?~~CENT L~V£~) 

.. •-------.--------------------------·-':"•--------------------------:--
.. ~j .. 

ao 

---------·--... ---------...................................... ------:------------·"'·---------------------..------ ST?.-.OO~flf) 

ue:·., r:.. nr.:tST ,l,IIOI.RU 

t' ~!HW ·"' l/1 PHI !'I 

,. ....... ,. .................... _ ............................... _ ..................... -.... ~--------------·-----------~-;-----------------

2:-t\:•:ll'iC•i 1 ~~ 3 J1.33JJ ;z.R'!oR 118B.U* l.oi:ots7 J".uuuu :S~.OUtHI 

2-M~ll'olJ/~ 3 lO.Uuou ti.l ij~4 &7.uliUlJU ... 725d 2JoU~\.f\,l .h.rJUII\.1 

UhSQ 3 20,.<:~6o7 4.7'2sa '22-3J334 2~721:!:!:i tl.IIOOU zo.uoon 

Sf' J li:!.UI)Oil J.OilOI} SdtlOGCi 1 "7 321 l:,.u.uitJ ll.;UOliU 

rr~ .. :t:~;::.o 3 22.001)7 '\.!11>38 i\ ~JJ3H 2oOf)tl1 20 .. UOtl0 2" .tiOIJU 

~"lf,t:./lUU 3 lc.OOOCI 1.01)00 ~ .. OOUt)Q 1.1l21 }J.UUI)U 1~n•Jil1JU 

F'Itll-.150 j H.obo7 ).7';.59 1 ..... JJJ)ol l .. l~~K 17.00U0 ~-t..•.n:tou 

FtREJ25 3 23 .. clbri7 o.so~"' 4:.£ .. .lJJ H .h7~b;, 17 .oouu JU.uUOO 

F~lU0/1000 3 71 .. 00il0 a.oooo b4 .. 000ll0 ... 6181:! c.l.IJUUU h.OOOU F~l00/500 3 42.0000 l.60Sb u .. uo·Ooo 2.0817 3ts.OOOO -lS.ooou 

FMtOO/tOU 3 31.0000 10.5357 11.1. I,)Ot)IJO o.oa2~ :n.oooo ·;lnUUDO 

FI-!100/SO 3 24.3JJJ 2.0817 4..33J~o\ 1.2019 22.00U0 2o .. t,)OOU 

-----·------·-~-------------........ .., ............................. ____ . __ ... _, .......... -.............................................. .., ........ . 

SOUFtC;: OF 

'I ~RLAT lOt' 

Ct:G<ZE.ES 

a, 

SUI>! Of SOUAli:~S [o"HfE.OOM 

.. 7b1563'i!f:>t-04 

• 712oouu:;.,.o3 

II 

24 .32lbci67S+02 

v "'R t.:..dc:; 

MAr to 

21.52 

I..E't't:L 

CtH 4 IC ilL UF 

F'-V :.LUC: S lC:'!l!:!C J.:1C!: 

l.1U • 01 

-----------------~..,,.. ..... ..,.,.,...,.., .. .., ..... ..,.., .. .., .. .,..., .. ..,..,..,.., .. .., .. .,...,w..,..,..,..,_,...,, .. ,.,..,,.._,.~••-----••------..---..-----

'IUTAL JS ________________ ......................................... -................................... ---------·---------------·----... ---·--- TUKEI'S ~Ut.tl?t.t: CUil?,\KlSUti 

------------~~------~-----~----------------------------~----------~-----~------~----- COH~Q!t ?OPIJL;\[lUNS 

C S ?!:RC:E:l•T L'i::V!::L) 

COBHOII PtJ()UL.:.TlOi•S. 

t 1 l"r:~c~wr t..&'./Et.l ________ ... ____ ,.. _______ ............................................... ,. ______ ... ___ ,. .................................... :-----·-------~-·---

·l 
F Zl 

J..b.d:id. Ill 0 • T E S T .!d.Y~ 1m:. 

STATISTICAL SUH~AkY 

............................ -...................................................................................... __ , .................. ;. ...................... _____ .., ... ________ 8TA!il)AM0 ST.4.:.0A.t!D 

___________ .t'Ff.:.\.1'f.'titt .,. _______ --. , _________ MEA .. ,...,,...,.., Dl-:'Jt~rilltl .. ,,.,u VARlst,.CI:: t:JHIUR :-t.t.'I1~UH r4AXI~liM .. _., ______________ .,...,..,.._ ............................. ----·------------

2•,'1./~ln0/10 3 4b .. 6bti7 1 .. 1~17 1.3JJ.J.i u.nao7 ~o.OOOO 4-d.UlJOU 

2-Ai•oi;.0/5 J JJ .. uooo :!).2915 21:i.OO!Hl0 3.055l 29.uouu J'LuO~O 

IJ''SU J ll.3J33 141547 l.JJJJJ d.i.lao7 12.U0Gu l"'~vouo Sk 3 1J. bnt) 7 .;.1633 11 .. JJJJJ 2.1037 9.0000 11 .c.•noo 

filH.:/250 J IO.lJl3 2.]Qq4 5 .. 3JJJl l.J))..\ 9 .. uooo lJ.ilOGO 

Hi!£:/ LOt) 3 9 .. J3lJ 1· 5 275 '2.J33JJ O.lf.s19 I:I.,OOQQ 11.0000 

Fllfi../50 3 12 .. 0000 ~ .1 9&2 27.6tJ000 J.ooou ti.OilUU l::i.OOOO 

ftil::.t'ZS l 12.,\JUUO 7~2111 S2~0ll0t1D •1-.1633 o.oooo 20.0000 

fHlOO/lUOO l u. 3JJJ J.:i119 12 .. JJ3ll :Z.O'Zlb a. .. ooou 15.0000 

F!otlOOJSOU 3 7.6bb7 1.'5215 ;l.JJJJJ u.lf819 b.OUOO 9~.:1000 

fM:.OO/lOU J 9.tlo07 2 .. 5166 b .. JJJJ.l 1.1530 7.0ll00 12.0UOO 

flollU0/50 l 9.btl&7 0.577~ O.,l~lJ3 Q.,llll 9.,\lOOU 10.,0U00 

_________ _.. .................................. ____ .................................................... _______ .,..., ____________________ . __ _ 

Al~.\L':!SIS Ct' YAHl.\~C!i: 

---.. "'·------------------------~-... --------------------------------------···--- SUUkC!. OF 

VAHUTtllN 

O!GR:i:ES 

Of FR~::oo:c 

MEAN 

SUUAH.:; 

V ~HU:tCC: 

HATICJ 

CH1TICM., 

F•II.\L.UI::: 

Lc:·n:!.. 

OF S!G.HF!Co\~C;: 

----------------------.-------~----------·---..... ~-~-- ...... --~ ................................. _______ ,. ____ ... <00 

.. 31 L9999£+U3 

II 

24 .lJOOOOCri::•Ol 

.H. 91 3 .. 10 

............ ____________________ .............................. ------------------·--------------------------- TO': At. JS ;:~ ----------·L------------------·---------------------------... -----... ------"':'--''"''"'--····-... , .... ------------- tUt.E~ ~S MUt.IIPLI:. C:U:UJ;..tt!S0.'1 

---------------··------------.. ·-------....... -----------------""''~·--------------- .. --··----- COMr.tON POPU.L.t.TIONS ' 

(S PE.RCZI>'t t.E'.''l~L) 

COMMON PUPUL~T!OhS 

(1 P~RC£N~ L~Y~Ll 

--------------------------------~----~-----------------------~---------------~------~ 
.:!Z. •' . 

--~---------~---------·-------~------------·--------------------------~--------------- S"t"AoiOA.RO STA'lUAIJD 

r~l::~T~I:.,.. r " Mt::A~ IJ~V J..l. r lGfl v 114 t;..:.cE E:i'tfflJK iHi'tl!'IU!fl :.tAA i:'IU:.; 

............................................................................ -..... -... ~ ..... ----.. ------------------""'-----------------

2 .. •\!•: lrit.d 1 0 l 2b2'!1 .. 6bo"l 29.7721 lliln!l7~1)t) 17 .. 1 tsbtt 2cOts .. UU(IU 2bcl .. UfiOu 

2-;.:'\Iti0/5 3 17~s.uuoo !Ja.'149t HH .. OOu.:lO 3.1..0343 t7'2.:l.00ti0 loJ!i.t.llHJO 

0~50 l 2fl.lJJ3 ... ~ 1)9'2, 'ZV.J.H33 2.ool34 ';t-\.OUoO jj.,UUfJu 

SR l 26.0000 b.SSH -"3 .lhHlUO 3.7ca5'J 2U.ouoo JJ.uuuu 

to·:rrt;;.::5o l 21.JJ33 ·z.~tno o .. Hl3ot 1,..1.:,]1,) 19.UUOO .o!.;.uOtHi 

flpr:/1oo J JO.Ouuo J.6056 \J.UI)(J(JQ 2.Uit17 21~0\100 J ... u0\10 

Fl .. e:ISO 3 2c. .. 6o07 1 .. 510o D. HJ.B 1 .. 1530 24.0000 ;t':J.OOOO 

FUU::/'25 3 -;zs ... ll.ll 3.7659 l•'hJ.U34 2.lli:,b 21 .. UOIJO 2d.V000 

f!HOO/lOUO 3 22.0000 3.0000 9.\)0IJOO 1. .. 7J:a "19 .. 0000 2~ .. oooo 

F1HD0/SOO J 25. JJ]J ).0551 9.3J3Jit 1.7b38 22.0QOO 2B.OOQO 

F~1001100 3 18.6607 0.5774 o .. 33.B" o .. JJJ3 lii .. OOOO u.oooo f~lOU/50 J 21 .. CIOOO 1 .. 0000 1 .. 00000 0 .. 5774 20 .. 0000 22.00UO 

---------~---------------------··---------,..------------------~----...... --~·-------·----

.. -----------~-.. -------·----·~----------.. ----··--... --.,;, ________ ----~---·-··---------·----

SCUFIC:!:: OF 

VAHUTltJ1i SUM Ol• S\OUAI'IC:S 

DEGiU:::s 

Of fBF.:~QO.""~ 

I..C:'i E:l. 

V"'H.U:tCE: CR11'1C.\L Of 

FIATlO F-VAt.UC: SlGU!F'I.C:.\r.C~ -----·-----------'*'·------"---------·--------------... -----------~--~----------- ..... ._ ___ _ .2-H!Si75t:+OS .225952JE ... o7 3.10 .01 

.3737063( ... 03 

------------------------------------~------~------·---~---~--------------------------- TOTAL 35 

---------------------·----· -·-----.. --................................................................................................................ ____ _ 

----------------~-..... ·------------~----- .. ----... --------~----------------------.. -~- .......... .. COM:CON POPULo1.tlONS COHMUfl PUI:JULATIOtiS 

TREU~r;NT 1i 1-\I::;U.. (5 P!::RCEilT t.ZVI::L) ( t P£NC£NT LI:::Yt;L) -----------------------.. ------------... --------------.; ________ !" ___________________ .,..,_ 

F'!-1100/100 

f1'1100/SO 

Fl~t::/2.50 

FM100/1U00 

FlftE/'.l5 

E'l-'lU0/500 

•• 

fIRE/50 

DfASU 

rt.REI LOO 

2•AHIMJ/S 

2.:.A;-o ltd.i/1 0 

l 

J 

J 

3 

3 

3 

3 

3 

3 

J 

J 

J 

\'-' 



... 

-« 

z 

-Ll. 

Ill 

a: -« 

fl) 

Ill 

... 

= ... 

... 

-« 

• Reproduced by N liS 

National Technical Information Service 

Springfield, VA 2216'1 

This report was printed specifically for your order 

from nearly 3 million titles available in our collection. 

For economy and efficiency, NTIS does not maintain stock of its 

vast collection of technical reports. Rather, most documents are 

custom reproduced for each order. Documents that are not in 

electronic format are reproduced from master archival copies 

and are the best possible reproductions available. 

If you have questions concerning this document or any order 

you have placed with NTIS, please call our Customer Service 

Department at 1-888-584-8332 or (703) 605-6050. 

About NTIS 

NTIS collects scientific, technical, engineering, and related 

business information- then organizes, maintains, and 

disseminates that information in a variety of formats- including 

electronic download, online access, DVD, CD-ROM, magnetic 

tape, diskette, multimedia, microfiche and paper. 

The NTIS collection of nearly 3 million titles includes reports 

describing research conducted or sponsored by federal 

agencies and their contractors; statistical and business 

information; U.S. military publications; multimedia training 

products; computer software and electronic databases 

developed by federal agencies; and technical reports prepared 

by research organizations worldwide. 

For more information about NTIS, visit our Web site 

at http://www.ntis.gov. 

• NTIS 

Ensuring Permanent, Easy Access to 

U.S. Government Information Assets 
¢OF "o 

l~"' .... , 

: 1Af ; '\4~/ '">47ES di 

US. DEPARTMENT OF COMMERCE 

Technology Administronon 

Nononol Technicollnformonon Service 

Springfield, VA 22161 (703) 605·6000 

111111111111111111111111111111111111111111111111111111111111 

1111111111111111111111111 

BIN: M30 06-27-07 

INVOICE: 1661132 

SHIPTO: 1*979351 

PAYMENT: CSH*V