# Sampling and Analysis Plan (SAP)

Quality Assurance Project Plan (QAPP)

For

Jacksonville Ocean Dredged Material Disposal Site (ODMDS) Site Designation Studies

Contract # W912EP

- 09

- R

- 0054

Submitted to

US Army Corps of Engineers

— Jacksonville District

701 San Marco Boulevard Jacksonville, Florida 32207

And

US Environmental Protection Agency, Region 4

Sam Nunn Atlanta Federal Center 61 Forsyth Street, SW Atlanta, Georgia 30303

Submitted by

ANAMAR Environmental Consulting, Inc.

2106 NW 67 th 

### Place, Suite 5 Gainesville, Florida 32653

- 1658

March 2010Sampling an d Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 1.0, Element A1: Title and Approval Sheet



### 2 

## GROUP A. PROJECT MANAGEMENT

1.0 

### ELEMENT A1

- TITLE AND APPROVAL SHEET

Title: 

### Jacksonville Ocean Dredged Material Disposal Site (ODMDS) Site Designation

Studies

Organization/Applicant: U.S. Army Corps of Engineers

— Jacksonville District

Project Officer: Glenn Schuster

Signature: Date:

Regulatory Agency: USEPA, Region 4

Designated Approving Official: Chris McArthur

Signature: Date:

Technical Review: Chris McArthur

Signature: Date:

Contractor: ANAMAR Environmental Consulting, Inc.

Project Manager: Michelle Rau

Signature: Date:

QA/QC Officer: Paul Berman

Signature: Date:Sampling an d Analysis Plan for Jacksonville ODMDS Site Designation Studies

Appendix

3 

### 2.0 ELEMENT A2

- TABLE OF CONTENTS

TABLE OF CONTENTS

1.0 ELEMENT A1

- TITLE AND APPROVAL SHEET

................................ ................................ .................. 2

2.0 ELEMENT A2

- TABLE OF CONTENTS

................................ ................................ ................................ .... 3

3.0 E LEMENT A3

- DISTRIBUTION LIST

................................ ................................ ................................ .... 8

4.0 ELEMENT A4

- PROJECT/TASK ORGANIZATION

................................ ................................ ............ 9

4.1 Survey Project Proponent

................................ ................................ ................................ ............ 9

4.2 Survey Project Team and Responsibilities

................................ ................................ ............ 9

5.0 ELEMENT A5

- PROBLEM DEFINITION/BACKGROUND

................................ ............................. 12

5.1 Purpose and Need/Site History

................................ ................................ ............................... 14

5.2 Identification of Principal Data Users and Decision Makers

................................ ....... 18

6.0 ELEMENT A6

- TASK DESCRIPTION

................................ ................................ ................................ ... 19

6.1 Purpose/Background

................................ ................................ ................................ .................. 19

6.2 Description of the Sampling and Analysis

................................ ................................ .......... 22

6.2.1. Measurements That Are Expected During the Course of the Sampling

Survey

................................ ................................ ................................ ............................ 22

6.2.2. Applicable Technical Quality Standards or Criteria

................................ ..... 24

6.2.3. Special Personnel or Equipment Requirements That May Indicate

the Complexity of the Dredging Project

................................ ........................... 25

6.2.4. Assessment Techniques Ne eded for the Dredging Project

....................... 25

6.2.5. Schedule for the Work Performed

................................ ................................ ...... 26

6.2.6. Required Deliverables/Reports

................................ ................................ ........... 26

7.0 ELEMENT A7

- QUALITY OBJECTIVES AND CRITERIA FOR MEASUREMENT DATA

......... 28

8.0 ELEMENT A8

- SPECIAL TRAINING REQUIREMENTS/CERTIFICATION

.............................. 31

9.0 ELEMENT A9

- DOCUMENTATION AND RECORDS

................................ ................................ ......... 32

9.1 Reporting of Results

................................ ................................ ................................ ..................... 32

9.2 Report Format

................................ ................................ ................................ ................................ . 33 Data Reporting Package Archiving and Retrieval

................................ ........................... 35

10.0 ELEMENT B1

- SAMPLING PROCESS DESIGN

................................ ................................ ................ 36

10.1 Scheduled Project Activities, Including Measurement Activities

.............................. 36

10.2 Rationale for the Design

................................ ................................ ................................ ............ 36

10.3 Design Assumptions

................................ ................................ ................................ ..................... 36

10.4 Procedures for Locating and Selecting Environmental Samples

............................... 36

10.5 Classification of Measurements as Critical or Noncritical

................................ ............ 41

10.6 Validation of Any Nonstandard Methods

................................ ................................ ............. 41

11.0 ELEMENT B2

– SAMPLING AND METHODS REQUIREMENTS

................................ ................... 42

11.1 Describe the Sample Collection, Preparation, and Decontamination Procedures

42

11.2 Identify Support Facilities for Sampling Methods

................................ ........................... 46

11.3 Describe Sampling/Measurement System Failure Response and

Corrective Action Process

................................ ................................ ................................ .......... 47

11.4 Describe Sampling Equipment, Sample Preservation, and Holding Times

............ 47

12.0 ELEMENT B3

– SAMPLE HANDLING AND CUSTODY REQUIREMENTS

................................ .. 52

13.0 ELEMENT B4

– ANALYTICAL METHODS REQUIREMENTS

................................ ......................... 54

13.1 Subsampling

................................ ................................ ................................ ................................ ... 54

13.2 Preparation of the Samp les

................................ ................................ ................................ ...... 54Sampling an d Analysis Plan for Jacksonville ODMDS Site Designation Studies

Appendix

4 13.3 Analytical Methods

................................ ................................ ................................ ....................... 54

13.3.1 Physical and Chemical Analysis

................................ ................................ ........... 54

13.3.2 Taxonomic Analysis

................................ ................................ ................................ .. 62

14.0 ELEMENT B5

- QUALITY CONTROL REQUIREMENTS

................................ ................................ .. 63

15.0 ELEMENT B6

– INSTRUMENT/EQUIPMENT TESTING, INSPECTION, AND MAINTENANCE REQUIREMENTS

................................ ................................ ................................ ........ 66

16.0 ELEMENT B7

– INSTRUMENT CALIBRATION AND FREQUENCY

................................ ............. 67

17.0 ELEMENT B8

– INSPECTION/ACCEPTANCE REQUIREMENTS FOR SUPPLIES AND

CONSUMABLES

................................ ................................ ................................ ................................ .......... 68

18.0 ELEMENT B9

– DATA ACQUISITION REQUIREMENTS (NON

- DIRECT MEASUREMENTS)

69

19.0 ELEMENT B10

– DATA MANAGEMENT, INTERPRETATION, AND REDUCTION

.................. 70

20.0 ELEMENT C1

– ASSESSMENTS AND RESPONSE ACTIONS

................................ ........................ 72

21.0 ELEMENT C2

– REPORTS TO MANAGEMENT

................................ ................................ .................. 73

22.0 ELEMENT D1

– DATA REVIEW, VALIDATION, AND VERIFICATION REQUIREMENTS

... 75

23.0 ELEMENT D2

– VALIDATION AND VERIFICATIONS METHODS

................................ .............. 76

24.0 ELEMENT D3

– RECONCILIATION WITH DATA QUALITY OBJECTIVES

............................... 77

2 5.0 REFERENCES

................................ ................................ ................................ ................................ .............. 78Sampling an d Analysis Plan for Jacksonville ODMDS Site Designation Studies

Appendix

5 

### List of Figures Figure 4

- 1 Project Organization Chart

Figure 5

- 1 Dredging Areas and ODMDS

Figure 5

- 2 Jacksonville Harbor Navigation Project

Figure 6

- 1 a Proposed Sampling Stations in North Candidate Site

Figure 6

- 1b Proposed Sampling Stations in South Candidate Site

List of Tables

Table 5

- 1 Five General and Eleven Specific Site Selection Criteria

Table 5

- 2 Project 10

- Year Status of the Jacksonville ODMDS

Table 5

- 3 Areas of Responsibility for Principle Dat a Users

Table 6

- 2 Estimated Schedule of Work Performed

Table 7

- 1 Data Quality Objectives for Sediment, Water, and Tissue Analyses

Table 7

- 2 Data Quality Objectives for Enumeration and Taxonomy of Invertebrates and

Fishes

Table 9

- 1 Laboratories Submitting D ata for this Project

Table 10

- 1 Sediment Sample Identification and Station Coordinates

Table 10

- 2 Macroinfaunal Sample Station Identification and Station Coordinates

Table 10

- 3 Macroepifaunal Sample Station Identification and Station Coordinates

Table 10

- 4 Physico chemical and Water Quality Station Identification and Station Coordinates

Table 10

- 5 Sample Analysis

Table 11

- 1 Standard Operating Procedures Used During Sampling

Table 11

- 2 Minimum Volume Requirements per Sample for Physical and Chemical Analysis

Table 11

- 3 Container Requirements for Sediment Samples for Chemical and Physical Analysis

Table 11

- 4 Container Requirements for Water Samples for Chemical Analysis

Table 11

- 5 Container Requirements for Tissue Samples for Chemical Analysis

Table 11

- 6 Contai ner Requirements for Macroinfaunal Samples for Taxonomic Analysis

Table 11

- 7 Container Requirements for Macroepifaunal Samples for Vouchering and

Verification of Identity

Table 12

- 1 Field Activity Forms Used to Document Sample Collection and Support Activi ties

Table 13

- 1 Analytes, Methods, and Target Reporting Limits: Sediment Physical Analysis

Table 13

- 2 Analytes, Methods, and Target Reporting Limits: Sediment Chemistry

Table 13

- 3 Analytes, Methods, and Target Reporting Limits: Water Chemistry

Table 13

- 4 A nalytes, Methods, and Target Reporting Limits: Tissue Chemistry

Table 14

- 1 Quality Control Activities Performed for Field, Physical, and Chemical Analyses

Table 21

- 1 Minimum Reporting Requirements

Table 21

- 2 Electronic Raw Data File FormatSampling an d Analysis Plan for Jacksonville ODMDS Site Designation Studies

Appendix

6 

### Appendices

Se diment Sampling Field Sheet

Water Quality Sampling Field Sheet

Macroinfaunal Sampling Log

Macroepifaunal (Trawl) Sampling Log

Chain

- of

- Custody Form

Daily Quality Control Report

Temperature LogSampling an d Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 2.0, Element A2: Table of Contents



### 7 List of Acronyms

CCL Contaminant Cleanup Target Levels

CQAR Ch emical Quality Assurance Report

DQCR Daily Quality Control Report

EPA U.S. Environmental Protection Agency

FDEP Florida Department of Environmental Protection

MDL Method Detection Limit

RL Reporting Limit

NELAC National Environmental Laboratory Association Conference

PAH Polynuclear Aromatic Hydrocarbons (also known as Polycyclic Aromatic

Hydrocarbons)

PCB Polychlorinated Biphenyls

QA/QC Quality Assurance/Quality Control

QAM Quality Assurance Manual

QAPP Quality Assurance Project Plan

SAP Sampling and Analy sis Plan

TBD To Be Determined

USACE U.S. Army Corps of EngineersSampling an d Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 3.0, Element A3: Distribution List Project /Task Organization



### 8 3.0 ELEMENT A3

- DISTRIBUTION LIST

This document is to be distributed to the following individuals for review and approval prior to

comm encement of sampling activities. Additional electronic co pies are available by request from ANAMAR Environmental Consulting, Inc.

1. USACE Project Officer: Glenn Schuster

2. USEPA Designated Approving Official: Chris McArthur

3. USEPA Technical Review: Chris McArthur

4. ANAMAR Project Manager: Michelle Rau

5. ANAMAR QA/Q C Officer: Paul BermanSampling an d Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 4.0, Element A4: Project/Task Organization

9 

### 4.0 ELEMENT A4

- PROJECT/TASK ORGANIZATION

4.1 Survey Project Proponent

Proponent: US Army Corps of Engineers

— Jacksonville District

Regulatory: US Environmental Protection Agency, Region 4

4.2 Survey Project Team and Responsibilities

O rganization: US Army Corps of Engineers

— Jacksonville District Project Officer : Glenn Schuster

701 San Marco Boulevard Jacksonville, Florida 32207 Phone: (904) 232

- 3691 Fax: (904) 232

- 1904

E

- mail: glenn.r.schuster@saj02.usace.army.mil Responsibilities: Project Officer for the Jacksonville ODMDS Site Designation Studies. Also responsible for co

- management of the Jacksonville ODMDS once approved for use by USEPA,.

Organization: US Environmental Protection Agency, Region 4 Designated Approving Official: Chris McArthur Wetlands and Marine Regulatory Section 61 Forsyth Street, SW Atlanta, Georgia 30303 Phone: (404) 562

- 9391 Fax: (404) 562

- 9343

E

- mail: mcarthur.christopher@epamail.epa.gov Responsibilities: Conduct designation survey of the proposed Jac ksonville ODMDS in accordance with 40 CFR 228.6(b) and approve or deny use based on survey results. Also responsible for co

- management of the Jacksonville ODMDS with USACE.

Organization: US Environmental Protection Agency, Region 4

Technical Review: Chr is McArthur

Coastal Section 61 Forsyth Street, SW Atlanta, Georgia 30303 Phone: (404) 562

- 9391 Fax: (404) 562

- 9343

E

- mail: mcarthur.christopher@epamail.epa.gov Responsibilities: Conduct designation survey of the proposed Jacksonville ODMDS in

accorda nce with 40 CFR 228.6(b) and approve or deny use based on survey results. Additionally, Chris will co

- manage the Jacksonville ODMDS with USACE.

Contractor 1: ANAMAR Environmental Consulting, Inc. Project Manager: Michelle Rau

2106 NW 67 th 

### Place, Suite 5 Gainesville, Florida 32653

- 1658 Phone: (352) 377

- 5770 ext. 107 Fax: (352) 378

- 7620Sampling an d Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 4.0, Element A4: Project/Task Organization

10 

### E

- mail: mrau@anamarinc.com Responsibilities: Project management, conduct designation survey, sample handling and transport, chains of custody, quality assurance manag ement, final data reporting Contracted by: USACE

— Jacksonville District under Contract # W912EP

- 09

- R

- 0054

Chemistry Laboratory 1: Columbia Analytical Services, Inc. (CAS) Project Manager: Jeff Christian

1317 S. 13th Avenue Kelso, Washington 98626

Ph one: (360) 501

- 3316 Fax: (360) 636

- 1068

E

- mail: jchristian@kelso.caslab.com Responsibilities: Sample holding and archiving, laboratory preparation and analysis for

sediment and site water. Contracted by: ANAMAR Environmental Consulting, Inc.

Geotechni cal Laboratory: MACTEC/Law Engineering Project Manager: Mark Coleman

3901 Carmichael Avenue Jacksonville, Florida 32207 Phone: (904) 396

- 5173 Fax: (904) 396

- 5703

E

- mail: macoleman@mactec.com Responsibilities: Sample holding and archiving, laborator y preparation and analysis for physical analyses. Contracted by: ANAMAR Environmental Consulting, Inc.

Taxonomic Laboratory: Barry A. Vittor & Associates, Inc. Project Manager: Linda W. Sierke 8060 Cottage Hill Rd. Mobile, Alabama 36695 Phone: (251) 6 33

- 6100

E

- mail: lsierke@bvaenviro.com Responsibilities: Macroin fauna identification and analysis. Contracted by: ANAMAR Environmental Consulting, Inc.

The successful completion of this project relies on open lines of communication between the client, contractor, regulatory agencies, laboratories, and subcontractors. This communication and successful completion of the project must be the contractor’s utmost goal. Contact information will be readily available throughout the life of this project, from pre

- planning to field work, data analysis, data reduction, and reporting. Any questions, clarifications, suggestions, and/or problems will be addressed in a timely manner.

See Project Organization Chart on next page.Sampling an d Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 4.0, Element A4: Project/Task Organization



### 11 

### Figure 4

- 1: Project Organization Chart

USACE Project Officer: Glenn Schuster (904) 232

- 3691

Geotechnical Lab:

MACTEC Project Manage r: Mark Coleman (904) 396

- 5173 Chemistry Lab:

CAS Project Manager:
Jeff Christian (360) 501

- 3316 USEPA Designated Approving Official:
Chris McArthur (404) 562

- 9391

Technical Review:
Chr is McArthur (404) 562

- 9391

Contractor: ANAMAR QA/QC Manager: Paul Berman (352) 377

- 5770 x106 Contractor: ANAMAR Project Manager:

Michelle Rau (352) 377

- 5770 x107

Infaunal Taxonomic Lab: Barry Vittor & Assoc. Project Manager:

Linda W. Sierke ( 251) 633

- 6100Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 5.0, Ele ment A5: Problem Definition/Background



### 12 5.0 ELEMENT A5

- PROBLEM DEFINITION/BACKGROUND

The U.S. Environmental Protection Agency, Region 4 (herein referred to as EPA) and U.S. Army Corps of Engineers

— Jacksonville District ( herein referred to as USACE) share responsibility for the control and management of ocean disposal of dredged material under the Marine Protection, Research, and Sanctuaries Act of 1972 (33 U.S.C. 1401 et seq., MPRSA). There are

three distinct but interre lated activities for which EPA and/or USACE have responsibilities with respect to ocean disposal of dredged material (USEPA and USACE 2007):

1) Designation or selection of sites for ocean disposal of dredged material,

2) Evaluation of the suitability of dr edged material for ocean disposal, and

3) Management and monitoring of Ocean Dredged Material Disposal Sites (ODMDSs) to ensure compliance with the MPRSA.

The MPRSA assigns basic responsibility to EPA and USACE for ensuring that ocean dredged

material d isposal activities will not unreasonably degrade or endanger human health, welfare, or amenities, or the marine environment (MRPSA Sections 102 and 103). Section 102 of the MPRSA authorizes EPA to designate sites or times at which dumping may occur and to establish criteria for reviewing and evaluating permit applications, including those for dredged material. It also authorized EPA, in conjunction with USACE, to develop site management plans for dredged material disposal sites. Section 103 of the MPRSA authorizes USACE to issue permits subject to compliance with the EPA environmental criteria (Ocean Dumping Criteria at 40 CFR Part 227) and EPA concurrence with USACE’s finding of compliance. Section 103(b) authorizes USACE, with EPA concurrence, to selec t alternative project sites of limited duration for disposal of dredged material in ocean waters when the use of a site designated by EPA is not feasible.

Site designation has two primary purposes. Sites are selected that minimize adverse

environmental e ffects and minimize the interference of dumping activities with other activities in the marine environment (USEPA 1986). Also, the site designation process is designed to ensure that temporary perturbations in water quality are reduced to normal ambient s eawater levels before reaching any beach, shoreline, marine sanctuary, or geographically limited fishery or shellfishery [40 CFR § 228.5 (a, b)].

A site designation requires a formal evaluation of alternative sites in which the potential environmental imp acts associated with disposal of dredged material at various locations are examined. Before the site designation process can begin, USACE must first demonstrate the need for the proposed ODMDS designation action [40 CFR 6.203(a) and 40 CFR 1502.13].

Once the need for an ocean disposal site is established, potential sites are screened for feasibility through the Zone of Siting Feasibility (ZSF) process. Once the ZSF process is completed, candidate sites are selected for further evaluation. S ites are eval uated using EPA’s ocean disposal criteria at 40 CFR Part 228. The regulations outline five general criteria and 11 specific factors upon which to base site selection (40 CRF 228.5

- 228.6; Table 5

- 1 ). Of the sites that satisfy these criteria, the site whic h best complies with the criteria is selected as the preferred alternative for formal designation through rulemaking published in the Federal Register.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 5.0, Ele ment A5: Problem Definition/Background



### 13 

### Table 5

- 1 Five General and 11 Specific Site Selection Criteria

General Site Selection Criteria

– 40 CFR 228.5

(a) The dumping of materials into the ocean will be permitted only at sites or in areas selected to minimize the interference of disposal activities with other activities in the marine environment, particularly avoiding areas of existing fisheries or shellfisheries, and regions of heavy commercial or recreational navigation.

(b) Locations and boundaries of disposal sites will be so chosen that temporary perturbances in water quality or other environmental conditions during initial mixing caused by disposal operations anywhere within the site can be expected to be reduced to normal ambient seawater levels or to undetectable contaminant concentrations or effects before reaching any beach, shoreline, marine sanctuary, or known geographically limited fi shery or shellfishery.

(c) If at any time during or after disposal site evaluation studies, it is determined that existing disposal sites presently approved on an interim basis for ocean dumping do not meet the criteria for site

selection set forth in Sec tions 228.5 through 228.6, the use of such sites will be terminated as soon as suitable alternate disposal sites can be designated.

(d) The sizes of the ocean disposal sites will be limited in order to localize for identification and control any immediate adverse impacts and permit the implementation of effective monitoring and surveillance programs to prevent adverse long

- range impacts. The size, configuration, and location of any disposal site will be determined as a part of the disposal site evaluation or designation study.

(e) EPA will, wherever feasible, designate ocean dumping sites beyond the edge of the continental

shelf and other such sites that have been historically used.

Specific Site Selection Criteria

– 40 CFR 228.6(a)

(1) Geographical pos ition, depth of water, bottom topography, and distance from the coast.

(2) Location in relation to breeding, spawning, nursery, feeding, or passage areas of living resources in adult or juvenile phases.

(3) Location in relation to beaches and other ameni ty areas.

(4) Types and quantities of wastes proposed to be disposed of, and proposed methods of release, including methods of packaging the waste, if any.

(5) Feasibility of surveillance and monitoring.

(6) Dispersal, horizontal transport, and vertical mixing characteristics of the area, including prevailing current direction and velocity, if any.

(7) Existence and effects of current and previous discharges and dumping in the area (including cumulative effects).

(8) Interference with shipping, fishing , recreation, mineral extraction, desalination, fish and shellfish culture, areas of special scientific importance, and other legitimate uses of the ocean.

(9) Existing water quality and ecology of the site as determined by available data or by trend asse ssment or baseline surveys.

(10) Potentiality for the development or recruitment of nuisance species in the disposal site.

(11) Existence at, or in close proximity to, the site of any significant natural or cultural features of historical importance.

So urce: USEPA 1986Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 5.0, Ele ment A5: Problem Definition/Background



### 14 5.1 Purpose and Need/Site History

The Jacksonville ODMDS is located on the continental shelf off Jacksonville, Florida, approximately 5 nmi southeast of the St. Johns River mouth ( Figure 5

- 1 ). It is 1 nmi by 1 nmi (1

nmi 2 

### ) in size with an average depth of 14 m (46 ft). Since 1952, the Jacksonville ODMDS and vicinity has been used for disposal of sand, silt, and clay dredge material from the Jacksonville Harbor Navigation Project, Naval Station Mayport entrance channel, and Naval Station Ma yport turning basin. The Jacksonville ODMDS received interim site designation status in 1977 and final designation in 1983.

Due to potential capacity issues at this site, the USACE

— Jacksonville District and EPA, Region

4 have identified a need to eithe r expand the Jacksonville ODMDS or designate a new ODMDS in the vicinity. The need for expanding current ocean disposal capacity is based on observed mounding, future capacity modeling, historical dredging volumes ( Table 5

- 2 ), and estimates of future prop osed projects. The purpose of the proposed action is to ensure that adequate environmentally acceptable and economically and logistically feasible ocean disposal site capacity is available for the next 50 years for suitable dredged material generated from new projects and maintenance dredging. The availability of suitable ocean disposal sites to support ongoing maintenance and capital improvement projects is essential for the continued use and economic growth of vital commercial and recreational areas in the region. As part of the site designation process, candidate sites are being evaluated based on environmental, operational, and economic criteria to identify a viable dredged material disposal site.

Disposal History and Future Needs

Prior to 1970 and i n the early 1970s, material was disposed in an area 0.5 nautical miles east of the Jacksonville ODMDS. In the late 1970s material was disposed south of the site (USEPA and USACE 2007). Naval Station Mayport has used the Jacksonville ODMDS regularly since 1994 and has been taking all of its maintenance dredged material to the Jacksonville ODMDS since two upland dredged material placement sites on Naval Station Mayport reached capacity in 1993. Since 1995, Naval Station Mayport has used the Jacksonville OD MDS on a biannual basis for disposal of most of its 900,000 cubic yards (cy) of maintenance material (annual average 450,000 cy) (NAVFAC 2008). Between 1996 and 2006, a total of approximately 4.8 million cy of dredged material was placed at the Jacksonvil le ODMDS, of which 82% was generated from Naval Station Mayport maintenance dredging ( Table 5

- 2 ) (USEPA and USACE 2007). Naval Station Mayport’s need for maintenance material disposal is expected to continue at increasing levels with deepening of the turn ing basin and entrance channel, and the Jacksonville ODMDS is the closest and preferred disposal site.

In addition, the Jacksonville Harbor Federal Navigation Project has used the Jacksonville ODMDS for disposal of coarse material not suitable for beach placement from the entrance channel. In 2007, approximately 510,000 cy of material was placed at the Jacksonville ODMDS for the federal navigation project ( Table 5

- 2 ). Currently, USACE removes approximately 300,000 cy from the outer portion of the feder al navigation channel during maintenance dredging every three years (annual average 100,000 cy), which has been disposed of in the Jacksonville ODMDS since 2003 (NAVFAC 2008). It is expected that Jacksonville Harbor Federal Navigation Project will continu e to utilize the ODMDS for non

- beach

- compatible material in the entrance channel. As upland disposal alternatives become limited, the volumes from the Jacksonville Harbor Federal Navigation Project may increase.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 5.0, Ele ment A5: Problem Definition/Background



### 15Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 5.0, Ele ment A5: Problem Definition/Background



### 16 

### Table 5

- 2 Projected 10

- Year St atus of the Jacksonville ODMDS

Maintenance Volume 460,000 cy (historical annual average) + 30,000 cy 1 (projected increase from proposed project) = 490,000 cy annual average

10

- Year Maintenance Volume 10 x 490,000 cy (average annual maintenance projects) = 4.9 million cy

Proposed NAVSTA Mayport Deepening 5.2 million cy

10

- Year Impact on Low

Volume Estimate: 4.9 million cy + 5.2 million cy = 10.1 million cy, which is 109% of available 9.3 million cy capacity of Jacksonville ODMDS

cy = cubic yards

1 The p rojected increase of 27,500 cy annual maintenance dredge increase resulting from the proposed deepening is conservatively rounded to 30,000 cy.

Source: NAVFAC 2008



### Recent sediment transport modeling indicated a sedimentation increase of about 2% within t he Naval Station Mayport turning basin, 7% within the Naval Station Mayport entrance channel, and 2% within the federal navigation channel (NAVFAC 2008). This equates to a ca. 5% increase of overall maintenance volume, or an annual increase of approximate ly 27,500 cy of maintenance material. In addition, it is estimated that the Jacksonville Harbor Federal Navigation Project will need to dispose of approximately 472,000 cy every three years for maintenance dredging due to shoaling at various sites along t he project reach.

New work is also being proposed, including the Department of the Navy homeporting of additional surface ships at Naval Station Mayport, which would require deepening of the turning basin and entrance channel at Naval Station Mayport an d deepening of the Jacksonville Harbor Federal Navigation Project, Bar Cut 3 ( Figure 5

- 1 ). Total estimated volume for dredging is approximately 5.2 million cy with subsequent disposals of maintenance dredged material of 0.8 million cy annually (NAVFAC 200 8). The proposed Jacksonville Harbor deepening project will likely increase annual maintenance volumes as well ( Figure 5

- 2 ). It is estimated that up to 38 million cy of material will be generated for potential new construction. There are also non

federa l projects that have dredged material disposal needs. In general, ODMDS use is expected to increase as upland disposal sites become limited.

Currently, use of the Jacksonville ODMDS is temporarily restricted to 2 million cy per year as

established in the 2007 Site Management and Monitoring Plan (SMMP) (USEPA and USACE 2007). This restriction is based on maximum historical uses of the ODMDS. Priority for ODMDS use within the capacity constraints will be given to maintenance projects from the Naval Statio n Mayport and the Jacksonville Harbor Federal Navigation Project. Approval for new work projects or maintenance material from non

- federal projects will be dependent on a consideration of site capacity and long

- term (i.e., 10 years) maintenance needs from the

aforementioned maintenance projects.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 5.0, Ele ment A5: Problem Definition/Background



### 17Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 5.0, Ele ment A5: Problem Definition/Background



### 18 It is recognized that the Jacksonville ODMDS requires expansion or a new site designated by EPA to enable continued disposal of maintenance dredging from Naval Station Mayport, the federal navigation channel, and others that use Jacksonville ODMDS; therefore, 10 years of maintenance capacity are used to determine viability. Table 5

- 2 provides an estimate of the

status of the Jacksonville ODMDS over the next 10

- year period based on historic use of the site,

the es timated remaining capacity using the low estimate of 9.3 million cy, and the 5.2 million cy of dredged material that would be generated from the proposed Naval Station Mayport deepening project. Under such a scenario, the 10

- year impact using the low volu me capacity estimate would result in 10.1 million cy being placed in the Jacksonville ODMDS, which represents 109% of its capacity.

5.2 Identification of Principal Data Users and Decision Makers

Table 5

- 3: Areas of Responsibility for Principle Data Users

Age ncy Organization Location Area(s) of Responsibility

USACE

— Jacksonville Jacksonville,

Florida Responsible for maintenance and co

- management of

the Jacksonville ODMDS with EPA, Region 4. Responsible for preparation of NEPA documents supporting new site desi gnation or expansion of the Jacksonville ODMDS pursuant to Section 103 of the

MPRSA.

USEPA,

Region 4 Atlanta, Georgia Responsible for new site designation ODMDS or expansion of the Jacksonville ODMDS. Gives concurrence to environmental requirements of dr edged sediment for approval for offshore disposal per the Green Book (USEPA 1991), SERIM (USEPA/USACE 1993), and the ITM (USEPA 1998), and co

- management
of the Jacksonville ODMDS with USACE

— Jacksonville.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 6.0, Element A6: Dredging Project/Task Description



### 19 6.0 ELEMENT A6

- TASK DESCRIPTION

6.1 Purpose/Background

The designation of an ODMDS will be executed by the EPA and USACE and will be based on:

Environmental studies of each site, regions adjacent to the site, and on historical knowledge of the impact of dredged material disposal on areas similar to such sit es in physical, chemical, and biological characteristics (Regulations and Criteria, Part 228.4)

Before final designation can be conferred upon a site, full environmental documentation must be prepared and approved by the EPA for each site (Pequegnat et al . 1990). All studies for the evaluation and potential selection of a new ODMDS will be conducted in accordance with five general criteria (40 CFR 22.5) and eleven specific criteria (40 CFR 228.6). The surveys described in this document are part of the re quired environmental documentation for the ODMDS site designation process.

Based on the ZSF analysis and available data, several study areas were selected for more in

depth evaluation to determine the suitability for dredged material disposal. In October 2009

and March 2010 , sidescan sonar survey s, video transects, and dives w ere conducted from the

EPA OSV Bold to assess the existence of hard or live bottom resources within and adjacent to these study areas . Based on results from this survey, EPA and US ACE selected two candidate

sites in which additional surveys described in this document will be performed ( Figure 6

- 1 )

.

The purpose of the site designation surveys is to further describe the environment of the candidate sites including sediment characteri stics (physical and chemical ), macroinfauna, macroepifauna , and water quality characteristics. The objective of these studies is to narrow down candidate sites to one preferred alternative based on the results. The goal of these surveys is to select the least environmentally damaging and most economically feasible and logistically practical site possible.

The information obtained during these surveys will be considered baseline data. Survey results will be used to determine whether future dredged mate rial placed at the new ODMDS has the potential to adversely impact benthic infaunal resources, benthic epifaunal resources, sediment quality, or water quality. Information gleaned from this study and future studies will be used to guide management decisio ns relative to future disposal at the selected site.

ANAMAR has been contracted by USACE to provide field and project management support to

USACE and EPA . ANAMAR will work as a team with USACE and EPA personnel to collect sediment, benthic, fish, and wat er quality samples for analysis of physical, chemical, and taxonomic parameters as outlined in this document. ANAMAR will manage subcontracting with laboratories and be responsible for transferring custody of samples to the appropriate

laboratories. ANAM AR will also receive the laboratory reports, review all results and quality control, compile data, and submit a complete report that summarizes results and conclusions.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 6.0, Element A6: Dredging Project/Task Description



### 20 

### FIGURE 6

- 1 a

. Proposed Sampling Stations in North Candidate Site.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 6.0, Element A6: Dredging Project/Task Description



### 21 

### FIGURE 6

- 1b. Pr oposed Sampling Stations in South Candidate Site.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 6.0, Element A6: Dredging Project/Task Description



### 22 6.2 Description of the Sampling and Analysis

Two site designation surveys are planned for this project. The first survey is planned for s pring 2010 (March 28

–

April 2)

, and the second is planned for f all 2010 ( September 13

– 18 ). The purpose of conducting two surveys is to account for seasonal variability of the parameters of interest. The two surveys vary slightly and will be described in more detail in the following sections. Objectives of the surveys focus on the characterization of selected areas of seafloor from a sedimentological, chemical, and biological pers pective, but also include physico chemical

studies of the water column in the selected areas.

6.2.1. Measurements That Are Expected During the Course of t he Sampling Survey

See Section 13.3 for proposed analytical methods and target detection limits.

Table 6

- 1 : Spring 2010 Field Sampling and Analysis Scheme for Jacksonville ODMDS

Site Designation Sampling Events

Sample Type Candidate

Site Number of Sample s Parameters / Analyses Collection

Method 1

Sediment North 7 + 1 field split = 8

Total = 8 samples Grain Size

TOC

Metals

PCBs

PAHs

Pesticides Organotins Grab

Sediment South 7 New Sediment Samples (3 additional stations have sediment analysis from prior s ampling) + 1 Field Split

Total = 7 + 1 = 8 Grain Size

TOC

Metals

PCBs

PAHs

Pesticides Organotins Grab

Sediment

Macroinfauna North 8 x 3 replicates = 24

Total = 24 samples Taxonomic ID

Various I ndices

Biomass Grab

Sediment

Macroinfauna South 10 Stations (4 previous + 6 New) to Match Sediment Stations x 3 Replicates

Total = 3 0 Samples Taxonomic ID

Various I ndices

Biomass Grab

Macroepifauna North 2 Stations x 2 Replicate Tows Inside

Site = 4 Tows

2 Stations x 2 Replicate Tows Outside

Site = 4 tows

Total = 8 samples Total B iomass

Taxonomic ID Species Richness

Other Indices Otter trawl

Macroepifauna South 2 Stations x 2 Replicate Tows Inside

Site = 4 Tows

2 Stations x 2 Replicate Tows Outside Total Biomass

Taxonomic ID Spec ies Richness

Other Indices Otter trawlSampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 6.0, Element A6: Dredging Project/Task Description



### 23 Sample Type Candidate

Site Number of Sample s Parameters / Analyses Collection

Method 1

Site = 4 tows

Total = 8 samples

Macroepifauna South Site Additional Tows if Time Permits Total Biomass

Taxonomic ID Species Richness

Other Indices Otter trawl

In Situ Water

Parameters North and

South Sites 1 S tation per S ite (within boundaries)

+ 2 Stations Outside Each Site

Total = 6 Profiles CTD Data 2

Temperature Salinity

Dissolved Oxygen Turbidity

PAR CTD

profiler

1 Details on collection methods are provided in Section 11

. 2 ANAMAR/Paul Berman processing data.



### Table 6

- 2 : Fall 2010 Field Samp ling and Analysis Scheme for Jacksonville ODMDS

Site Designation Sampling Event

Sample Type Candidate

Site Number of Samples Parameters / Analyses Collection

Method 1

Sediment

Macroinfauna North 8 x 3 Replicates = 24

Total = 24 samples Taxonomic ID

Variou s Indices

Biomass Grab

Sediment

Macroinfauna South 10 Stations (4 previous + 6 New) to Match Sediment Stations x 3 Replicates

Total = 30 Samples Taxonomic ID

Various Indices

Biomass Grab

Macroepifauna North 2 Stations x 2 Replicate Tows Inside

Site = 4 Tows

2 Stations x 2 Replicate Tows Outside

Site = 4 tows

Total = 8 samples Total Biomass

Taxonomic ID Species Richness

Other Indices Otter trawl

Macroepifauna South 2 Stations x 2 Replicate Tows Inside

Site = 4 Tows

2 Stations x 2 Replicate Tows Outside

Site = 4 tows

Total = 8 samples Total Biomass

Taxonomic ID Species Richness

Other Indices Otter trawl

Macroepifauna South Additional Tows if Time Permits Total Biomass

Taxonomic ID Species Richness

Other Indices Otter trawl

Macroepifaunal Tissue Analys es North and

South Sites 4 samples x 2 species + 1 field dup = 9 per site Metals

PCBs

PAHs Samples

processed

atSampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 6.0, Element A6: Dredging Project/Task Description



### 24 Sample Type Candidate

Site Number of Samples Parameters / Analyses Collection

Method 1
Total = 18 samples between the 2 sites

TBD or Same as Above Pesticides laboratory

In Situ Water

Parameters North and

South Sites 1 Station per Site (within boundaries)

+ 2 Stations Outside Each Site

Total = 6 Profiles CTD Data 2

Temperature Salinity

Dissolved Oxygen Turbidity

PAR CTD

profiler

Water Quality North and

South Sites 2 Stations (flood and ebb) per Site x 2

Samples per Station (1 sampl e above thermocline and 1 sample near

bottom)

+ 1 field duplicate per site

Total = 9 samples Metals

PAHs

Pesticides

PCBs

TSS Niskin

Bottles

1 Details on collection methods are provided in Section 11

. 2 ANAMAR/Paul Berman processing data.



### 6.2.2. Applicable Techni cal Quality Standards or Criteria

Physicochemical Sediment Analysis

— Sediment results will be compared to published sediment screening values where appropriate. These levels are the threshold effect level (TEL) and the effects range

- low (ERL). The TEL r epresents the concentration below which adverse effects are expected to occur only rarely. The ERL is the value at which toxicity may begin to be observed in sensitive species (Buchman 2008). Comparisons will be used for reference only

and are not intend ed for any regulatory decisions. The main objective of the sediment physical and chemical analyses is to help provide characteristics from which to compare the two
candidate sites.

Sediment Macroinfauna Survey

— In this document, the term ‘macroinfauna’ r efers to those organisms greater than 0.5 mm in length which live in sediment. Major taxonomic groups which fit the macroinfauna description include polychaete worms (e.g., lugworms, sandworms), bivalve molluscs (e.g., scallops, clams, oysters, mussels), amphipod crustaceans (also known as scuds), and sipunculid worms (also known as peanut worms).

Data derived from the benthic macroinvertebrate (>0.5 mm size) infaunal community study is intended to help determine the biological characteristics of each pro posed ODMDS site. Results of the study will be included in the preparation of an Environmental Impact Statement (EIS) where potential impacts w ill be assessed and discussed.

Macroepifauna Survey

— In this document, the term ‘macroepifauna’ or ‘epifauna’ re fers to those organisms which live and/or feed primarily on or near the seafloor and which are of a size which allows capture and retention in a 7.3

- meter otter trawl . Organisms fitting this description

include echinoderms, shrimps, lobsters, crabs, and d emersal (both benthic and benthopelagic)

fishes.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 6.0, Element A6: Dredging Project/Task Description



### 25 Results of the trawl

- based macroepifaunal survey are intended to assess the invertebrate community and fish assemblages associated with the seafloor. Similar to the macroinfauna survey, these data are inte nded to characterize each site and be used to compare the two candidate sites. These data will additionally be included in an EIS

. Results of tis sue analysis of

selected fish and invertebrate species are intended to assist with the comparison of the two
candidate sites.

Physi c ochemical Water Studies

— In situ parameters and water quality results will be used

to characterize each site. These data will be included in comparisons of the two candidate

sites. Additionally, these data will be used in future A u tomated D redging and D isposal

A lternatives Modeling System S imulations for dredged material disposal events to document compliance with EPA marine water quality criteria

.

6.2.3. Special Personnel or Equipment Requirements That May Indicate

the Complexity of the Dredging Project

Use of the OSV Bold will be required for performing surveys. The sampling equipment will be operated by Bold personnel. In addition to personnel provided by EPA and USACE, two sen ior

scientists and one fish biologist will be provided by ANAMAR for the s pring 2010 survey. One

se nior scientist and one fish biologist will be provided by ANAMAR for the fall 2010 survey. ANAMAR may will als o provide two collection technicians from the Florida Museum of Natural History having suitable special ized profession training which qualifies them to help sort macroepifaunal samples , and will be directly supervised by an ANAMAR scientist.

6.2.4. Assessment Techniques Needed for Site Designation Studies

Sediment Physicochemical Analysis

— Sediment collected will be analyzed for physical and chemical parameters in the laboratory and then validated against criteria established in this document. The assessment techniques stated in sections 20 and 23 are adequate to provide sufficient assurance that the quality objec tives of the project will be met.

Sediment Macroinfauna Survey

— Benthic infaunal invertebrates greater than 0.5 mm collected in benthic grab samples will be sorted and identified to lower taxonomic group in the laboratory at Barry A. Vittor & Associates, Inc. and will be assessed using techniques stated in

sections 20 and 23 of this document. The information contained within these sections is designed to provide adequate assurance that quality objectives will be met.

Macroepifauna Survey

— Epibenthic inv ertebrates and demersal fishes collected during the

otter trawl survey will be sorted and determined to lowest taxonomic group by ANAMAR

scientists . The assessment techniques discussed in sections 20 and 23 of this document are designed to meet quality ob jectives of the project.

In Situ Water Parameters

— In situ water parameters will be recorded using a CTD profiler during field operations aboard the OSV Bold

. Data recorded with the CTD will be post

processed back in the office. Assessment techniques wil l include those stated in sections 20 and 23 of this document and are designed to provide sufficient assurance that quality objectivesSampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 6.0, Element A6: Dredging Project/Task Description



### 26 of the project will be met.

Water Chemistry Analysis

— W ater samples from each candidate site will be collected and

sent t o Columbia Analytical Services, Inc. for laboratory analysis of selected chemical components using analytical methods specified in Section 13 of this document. Assessment techniques used in water analyses are stated in sections 20 and 23 of this document and are designed to provide sufficient assurance that quality objectives of the project will be met.

6.2.5. Schedule for the Work Performed

The spring 2010 survey is planned for the period March 28

– April 2 , 2010. The fall 2010 survey is planned for the period S eptember 13

– 18 , 2010. The following table provides an estimated schedule for the completion of the work.

Table 6

- 3 : Estimated Schedule of Work Performed

Responsibility Estimated Schedule for Sampling/Testing/Reporting Estimated Date

ANAMAR Submit draf t SAP/QAPP to EPA and USACE for approval. Allow FDEP to comment March 20 10

ANAMAR Address comments and finalize SAP/QAPP March 20 10 USACE/EPA/ANAMAR Conduct spring 2010 site designation study March 28

– April 2 , 2010

ANAMAR Submit sediment, water, and ma croinfaunal samples to laboratories for analysis April 2010

Laboratories (see section 4.3) Submit spring 2010 analytical reports to

ANAMAR July 2010

ANAMAR Review data from spring sampling event: analytical reports, begin compiling data tables,

maps, an d figures

. July

– August 2010

USACE/EPA/ANAMAR Conduct fall 2010 site designation study September 13

– 18 , 2010

ANAMAR Submit macroinfauna and tissue samples to laboratories for analysis September 2010

Laboratories (see section 4.3) Submit fall 2010 analyt ical reports to ANAMAR December 2010

ANAMAR Review analytical reports, compile data tables, maps, and figures, and produce draft report for submission to USACE/EPA Early January 201 1

USACE and EPA Review draft report and submit comments to

ANAMAR J anuary 2011

ANAMAR Respond to comments by USACE and EPA January

– February 2011

ANAMAR Submit final report containing results of both studies to USACE/EPA February 2011



### 6.2.6. Required Deliverables/Reports

The following documents must be submitted:

1. Draft Sampling and Analysis Plan/Quality Assurance Project Plan (SAP/QAPP) submitted

for review and comment.

2. Final SAP/QAPP following update from comments for final approval prior to sampling.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 6.0, Element A6: Dredging Project/Task Description



### 27 3. Field paperwork includes field sheets, Daily Quality Control Report, calib ration sheets,

etc. These sheets are described in Table 12

- 1 and are provided in Appendix B of this document. They are also provided as an electronic attachment on a compact disk.

4. Chemical Quality Assurance Report (CQAR). The CQAR is used to evaluate al l of the representative data from the project field sampling and laboratory analyses. For each group of data, a data review checklist is completed that assesses daily field QC reports and specific QC chemical data quality indicators, and it enables the re viewer to identify potential data problem areas that may require additional data validation. The Quality Assurance Report identifies non

- conformances, QC deficiencies, or other problems that would impact the data quality objectives as specified in the wor k plan and the QAPP. The CQAR summarizes the overall usability of the data for the intended purposes. This report will be an appendix to the Final Report.

5. Draft Report submitted for review and comment

— this report must include all supporting

documentation , including field paperwork, laboratory reports, the chemical quality assurance report, and all correspondences among ANAMAR, USACE, and EPA which address any modifications to the project.

6. Once approved by USACE and EPA, the Final Report will be submitted which will address

all comments.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 7.0, Element A7: Quality Objectives and Criteria for Measurement Data



### 28 7.0 ELEMENT A7

- QUALITY OBJECTIVES AND CRITERIA FOR MEASUREMENT

DATA

Table 7

- 1: Data Quality Objectives for Sediment, Water, and Tissue Analyses

Parameter QC

Measurement Frequency Acceptance Criteria Storage/Holding

Times

All organic analyses

PAHs, PCBs,

and pesticides (including

tissue analysis) MB 1 per 20 samples or 1 per batch up to 20 samples No analyte should be

detected > RL 14 days until extraction, 40 days thereafter

MS/MSD 1 set per 20 samples

or 1 set per bat ch up to 20 samples 70

– 130% for spike limits 30% RSD for precision

Duplicate 1 per 20 samples or 1

per batch up to 20 samples 30% RSD for precision (Evaluated for analytes >300% RL)

SRM** 1 per 20 samples or 1

per batch up to 20 samples Within limi ts specified by provider (Evaluated for analytes >300% RL)

ICV Immediately following

calibration curve 80

– 120% Recovery

CCV Minimum

— one per 10

samples and at the

end of each batch

whenever batch is greater than 10 or for

GC/MS at the beginning of e very 12

hours RRF or RF ≤25% for

GC/MS methods and

≤15% for all other

methods

Surrogates Every sample 30

– 150% Recovery

Internal

Standard Every sample, where

applicable 50

– 200% Recovery

IC Verify after each initial

calibration <20% RSD for each analyte or RF ≤30% for

GC/MS

MDL Verify MDL study

once per year for each analyte of

interest Updated annually

Metals (including

tissue analysis) MB 1 per 20 samples or 1 per batch up to 20 samples No analyte should be

detected > RL 180 days

MS/MSD 1 set per 2 0 samples

or 1 set per batch, up to 20 samples 70

– 130% for spike limitsSampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 7.0, Element A7: Quality Objectives and Criteria for Measurement Data



### 29 Parameter QC

Measurement Frequency Acceptance Criteria Storage/Holding

Times

Duplicate 1 per 20 samples or 1

per batch up to 20 samples 30% RSD (Evaluated for analytes >300% RL)

SRM 1 per 20 samples or 1

per batch up to 20 samples 70

– 130% Recovery (Eval uated for analytes >3x RL)

LCS/LFB 1 per 20 samples or 1

per batch up to 20 samples 70

– 130% Recovery

ICV Immediately following

calibration curve 90

– 110% Recovery

CCV Minimum

— one per 10

samples and at the

end of each batch

whenever batch is

grea ter than 10 90

– 110% Recovery

LDR Verify LDR once per

quarter for ICP analysis and one time for mercury analysis Refer to frequency

Initial Calibration for AA, Hg Performed daily Correlation coefficient ≥

0.995

MDL Verify MDL study

once per ye ar for each analyte of

interest Updated annually

ICB Immediately after

initial calibration No analyte should be

detected > RL

TOC MB 1 per 20 samples or 1 per batch up to 20 samples No analyte should be

detected > RL 28 days

MS/MSD 1 set per 20 sa mples

or 1 set per batch up to 20 samples 75

– 125% for spike limits

20% RSD for precision (Evaluated for analytes >300% RL)

Triplicate 1 per 20 samples or 1

per batch up to 20 samples 20% RSD for precision (Evaluated for analytes >300% RL)Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 7.0, Element A7: Quality Objectives and Criteria for Measurement Data



### 30 Parameter QC

Measurement Frequency Acceptance Criteria Storage/Holding

Times

SRM* 1 p er 20 samples or 1

per batch up to 20 samples Within limits specified by provider (Evaluated for analytes >300% RL)

ICV Immediately following

calibration curve 80

– 120% Recovery



### CCV Minimum

– one per

10 samples and at the

end of each batch

whenever batch is greater than 10 90

– 110% Recovery



### IC Verify after each initial

calibration CC > 0.9950 for all

calibrations



### MDL Verify MDL study

once per year for each analyte of

interest Updated annually



### Grain Size Triplicate 1 set per 20 samples

or pe r batch <20% RSD Undetermined

* If SRMs are not available, use laboratory control samples

.



### Table 7

- 2: Data Quality Objectives for Enumeration and Taxonomy of

Invertebrates and Fishes

Parameter QC

Measurement Frequency Acceptance Criteria Storage/Holding

Times

Verification of

Taxonomic

Identification

and

Enumeration Macroinfauna

(invertebrates) > 10% of samples

sorted by each person

will be re

- sorted and

re

- identified by a qualified taxonomist > 95% accuracy of sorting

and identification Undetermined (not critical)

Macroepifauna (Invertebrates) 10% of

specimens/station re

identified by qualified biologist Zero (0) misidentifications Undetermined (not critical)

Macroepifauna (Fishes) 10% of

specimens/station re

identified by qualified biologist Zero (0 ) misidentifications Undetermined (not critical)Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 8.0, Element A8: Special Trai ning Requirements/Certification



### 31 8.0 ELEMENT A8

- SPECIAL TRAINING REQUIREMENTS/CERTIFICATION

All sampling and field work will conform to the USACE Safety Manual EM 385

- 1

- 1 (USACE 2003).

All members of the sampling crew shall be trained i n the correct procedures to ensure the samples collected are representative of the area and are not contaminated during handling. This training shall occur prior to any sampling. EPA will address all sampling procedures, while ANAMAR will address procedu res for sample handling directly related to homogenizing and compositing the sediment, and placing in sample containers. Taxonomic identification and enumeration of benthic macroepifauna will be performed by a staff biologist specifically qualified throug h a combination of formal training and professional experience to execute such

tasks.

All ANAMAR field personnel are trained in cardiopulmonary resuscitation (CPR) and first aid. Certification must be updated annually for CPR and every three years for fi rst aid. This training is required for anyone participating in field work. Training records are maintained at ANAMAR headquarters and each employee is provided with a card to carry indicating their training.

All laboratory personnel are required by NEL AC to have a signed, dated initial demonstration of capability for any analysis they perform. Each initial demonstration of capability is required to be updated annually. A document signed by the lab director, QA officer, and analyst is stored

in the ana lyst’s records to verify both initial and continuing demonstration of capability.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 9.0, Element A9: Documentation and Records



### 32 9.0 ELEMENT A9

- DOCUMENTATION AND RECORDS

9.1 Reporting of Results

Table 9

- 1: Laboratories Submitting Data for this Project

Laboratory Analyses to be

Performed Data Package Desc ription

ANAMAR In situ field

parameters, epifaunal

invertebrates and

fishes ANAMAR will compile in situ data into data tables and

submit as part of the report

Invertebrate and f ish identification, enumeration, lengths, indices, etc. results summarized and discussed in report

Columbia Analytical

Services Metals, PAHs,

PCBs, pesticides, TOC (sediment, water, tissue samples) Data summary, initial calibration and verification, quality

control including blanks, LCSs, duplicates, spikes, control limits, sample case narrative, and raw data package from instruments

MACTEC Physical

parameters of

Sediment Data summary, quality control including duplicates and

triplicates, and sample case narrative if applicable

Barry Vittor and Associates, Inc. Macroinfaunal

surve y Taxonomic identification and enumeration data

summary, quality control, and narrative if applicable

ANAMAR will provide a summary of each laboratory’s results as an appendix to the report. The tabular formatted summary will include sample identificat ion, analyte, results, limits for comparison, and qualifiers. Due to their large size, all original laboratory results will be submitted only as electronic files.

The data obtained will be presented in graphical, tabular, and/or written text as appropr iate. The draft and final testing reports will undergo internal technical review and quality assurance review by persons with appropriate technical qualifications to ensure that the report meets the project requirements specified in the technical work pl an and the QA goals.

The report(s) will consist of standard size, 81⁄2

- inch by 11

- inch, pages with oversize figures or tables folded, if necessary, to this size. The report margins shall be suitable for use in a durable

3

- ring binder. A decimal numberin g system will be used, with each section having a unique decimal designation. Reports that require extensive editing, have extensive errors, or are not in the required formats will be rejected and re

- submittal will be required. All submittals shall be

s ent to USACE

— Jacksonville District and EPA, Region 4. Any maps, drawings, figures, sketches, databases, spreadsheets, or text files prepared for this report shall be provided in both hard copy and digital form.

The digital copies of reports and other t ext documents shall be provided in Microsoft Word 2000 (or higher version). Spreadsheet files and data files shall be provided in Microsoft Excel 2000 (or higher version) format. All text, spreadsheet, and database files shall be delivered in compact dis k read

- only memory (CD

- ROM) with ISO

- 9660 format. Level IV laboratory dataSampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 9.0, Element A9: Documentation and Records



### 33 shall be provided as Adobe Acrobat portable document format (PDF) files.

Geographic data , including coordinates of all sampling stations, shall be provided in feet and projecte d into the Florida State Plane coordinate syste m

.

One hardcopy and one electronic copy (provided on compact disk) o f the final report shall be

submitted to USACE

— Jacksonville District and EPA, Region 4. Other interested agencies or
persons may request a copy of the final report. This report will also be included as an appendix to the EIS that will be completed for the Jacksonville ODMDS site designation project.

9.2 Report Format

The following paragraphs represent the format for electronic files being deliv ered as part of any contract. These paragraphs do not specify content or what the electronic files should contain. The content or data represented should be specified in the scope of work.

1. Specifications for Digital Data . Any maps, drawings, figures, sketches, databases, spreadsheets, or text files prepared under the terms of this contract shall be provided in both hard copy and digital form, unless otherwise specified in the scope of work. The hard copy deliverables shall be defined in the body of t he scope of work.

2. Text, Spreadsheet, and Database Files : The USACE

— Jacksonville District standard computing software is Microsoft Office. Reports and other text documents shall be provided in Microsoft Word 2000 (or higher version) format and Adobe Acr obat Portable Document Format (PDF). Spreadsheet files shall be provided in Microsoft Excel 2000 (or higher version) format. Databases shall be provided in Microsoft Access format, unless otherwise specified in the scope of work. Prior to database devel opment, the contractor shall provide the Government with a Technical Approach Document and Entity Relationship Diagram for approval which describes the contractor's technical approach to designing and developing the database. All text, spreadsheet, and da tabase files shall be delivered on compact disk read

- only memory (CD

- ROM) with ISO

- 9660 format.

3. Digital Mapping and Data Standards : The USACE

— Jacksonville District utilizes MicroStation for computer

- aided design and drafting (CADD). Data provided must be readable by MicroStation SE or higher to provide design drawings, sketches, or figures. All digital files provided in MicroStation shall be provided in feet and projected into the Florida State Plane coordinate system. The maps shall use the GRS 1980 spheroid and the North American Datum of 1983 (NAD 83), and shall be provided on CD

- ROMs.

4. Geographic Information System (GIS) Data Delivery Format

a. Digital geographic maps and the related digital information shall be developed using double precision and delivered in uncompressed ArcInfo export file format (.e00) using ArcInfo Version 8.0 or higher. USACE

— Jacksonville District will also accept ArcView shapefiles (e.g., .shp, .shx, .dbf). These file formats are geographic information system software a pplications produced by the Environmental Systems Research Institute and are in the GIS software suite used by USACE

— Jacksonville

District and EPA , Region 4

. b. Digital geographic maps and the related digital information shall be usable on an

IBM

- compatibl e personal computer system using the Windows NT 4.0 or WindowsSampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 9.0, Element A9: Documentation and Records



### 34 2000 operating systems. This data shall be provided on compact disk read

- only memory (CD

- ROM) with ISO

- 9660 format.

5. General Digital Standard for CADD and GIS Files

a. Geographic data shall be provided in feet and projected into the Florida State Plane coordinate system. The maps shall use the GRS 1980 spheroid and NAD 83. Vertical upland topographic surveys shall use the North American Vertical Datum of 1988 (NAVD 88). Hydrographic survey will reference the local dredging datum which will be provided in the project scope of services. No offsets shall be used. Each map layer or coverage shall have a projection file. Map or drawing scales will be determined by the Contracting Officer's Re presentative for the contract. Mapping accuracy for the agreed scales will conform to the American Society for Photogrammetry and Remote Sensing (ASPRS) Accuracy Standards for Large

- Scale Maps and Interim Accuracy Standards for Large

- Scale Maps (ASPRS 1991 ). Copies of the ASPRS Accuracy Standards can be obtained by contacting:

American Society for Photogrammetry and Remote Sensing

5410 Grosvenor Lane, Suite 210 Bethesda, Maryland 20814

- 2160

b. Geographic data must be provided in a form that does not requir e translation, preprocessing, or post processing before being used in the USACE’s system. However, the Contractor shall consult with the Government (specifically the Geographic Information Systems Coordinator) concerning the use of alternative delivery for mats such as MicroStation SE or higher to provide design drawings, sketches, or figures. All digital files provided in MicroStation shall be in the same projection and use the same coordinate system, datum, and units as stated above, and shall be provided on CD

- ROMs.

c. Geographic Data Structure: All geographic information shall be developed in a structure consistent with the Spatial Data Standards (SDS), Version 1.9or a higher version if available at the time of this project. The Contractor shall consul t with the Government concerning modifications or additions to the SDS. The Government may approve modifications to the Standard if it is determined that SDS does not adequately address subject data sets. Copies of the SDS may be obtained by contacting:

Director, US Army Engineer Waterways Experiment Station

Tri

- Service CADD/GIS Technology Center

Attn: CEWES

- IM

- DA/Smith 3909 Halls Ferry Road Vicksburg, Mississippi 39180

- 6199

d. Geographic Data Documentation: For each digital file delivered containing

ge ographic information (regardless of format), the Contractor shall provide

documentation consistent with the Content Standards for Digital Geospatial Metadata, June 1998 published by the Federal Geographic Data Committee. The

documentation shall include bu t is not limited to the following: the name and description of the map layer or coverage, the source of the data and any related data quality information such as accuracy and time period of content, the type ofSampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 9.0, Element A9: Documentation and Records



### 35 data coverage (point, line, polygon, etc.), the field names of all attribute data and a description of each field name, the definition of all codes used in the data fields, the ranges of numeric fields and the meaning of these numeric ranges, the creation date of the map layer and the name of the pe rson who created it. A point of contact shall be provided to answer technical questions. Metadata generation software, referred to as Document.aml, is available from ESRI for use with ArcInfo to help in the production of the required metadata. Corpsmet9 5 metadata software is available from the US Army Geospatial Clearinghouse at http://corpsgeo1.usace.army.mil/

. Copies of the FGDC metadata standard can be obtained by contacting:

FGDC Secretariat c/o U.S. Geological Survey

590 National Center Reston, Virginia 22092 (703) 648

- 5514

FGDC metadata standards can also be found on the Internet at:

http://www.fgdc.gov

e. Geographic Data Review: The digital geographic maps, rel ated data, and text

documents shall be included for review in the draft and final contract submittals. The reviews may include a visual demonstration of the geographic data on the Windows NT computer system in the Environmental Resources Section GIS Unit' s. Actual installation of the digital data from the CD

- ROM onto the computer will be conducted by GIS Unit personnel. However, the Contractor shall have a technical consultant available at each review to assist with any digital data discrepancies. The

d ata will be analyzed for subject content and system compatibility. Review comments to data and text shall be incorporated by the Contractor prior to approval

of the final submittal.

f. Ownership: All digital files, final hard

- copy products, source data a cquired for this project, and related materials, including that furnished by the Government, shall become the property of USACE

— Jacksonville and will not be issued, distributed, or published by the Contractor.

9.3 Data Reporting Package Archiving and Retr ieval

All reports, data, field sheets, correspondence, notes, field books, and any other documents associated with this project will be archived by the contractor for a minimum of five years from the date of the final report. Prior to disposal of any reco rds, the contractor must contact the client (USACE

— Jacksonville District) for authorization and direction in the disposal of said

documents.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 11.0, Element B2: Sampling and Methods Requirements



### 36 

## GROUP B. PROJECT MANAGEMENT

10.0 ELEMENT B1

- SAMPLING PROCESS DESIGN

10.1 Scheduled Project Activities, Including Meas urement Activities

The spring 2010 survey is scheduled for the period March 28

– April 2 , 2010. The fall 2010 survey is sch eduled for the period September 13

– 18 , 2010. Each survey is estimated to take

four da ys plus one day of mobilization and one day of d emobilization per survey

.

Samples will be packed and transported to the respective laboratories immediately after return

from the field.

10.2 Rationale for the Design

Sections 5.0 and 6.0 of this document describe the rationale for conducting these site desig nation studies. Sample sites and parameters we re determined in accordance with guidance from Section 102 and 103 of the MPRSA and 40 CFR 228

, Revised Procedural Guide for Designation Surveys of Ocean Dredged Material Disposal Sites (Pequegnat, 1990) , and

SERIM (USEPA and USACE 2008)

.

10.3 Design Assumptions

All sampling will be conducted under the direct supervision of EPA , Region 4 and ANAMAR . EPA and USACE will address any concerns with field sampling and the possible relocation of sample sites, including, but not limited to, the foreseen problems discussed below.

Possible foreseen problems and solutions include the following:

Problem: Rock at a depth not allowing sediment sample collection. Solution: Relocate sample location.

Problem: Heavy traffic in the sampling area limiting sample collection. Solution: Relocate sample location(s), postpone sampling, or sample around traffic (safety dependent).

Problem: Adverse weather (e.g., hurricane, lightning, heavy rain) and/or rough seas. Solution: Postp one sampling until the situation clears.

All changes to the sampling protocol specified by this QAPP and the EPA QAPP will be logged on

the field sheets.

10.4 Procedures for Locating and Selecting Environmental Samples

Sample stations for the collection of sediment, water, faunal tissue, macroinfauna, and macroepifauna samples are listed below. Sample station locations within the study areas were determined by EPA and USACE

— Jacksonville District. The sample identifications and coordinates are organized be low in Tables 10

- 1 through 10

- 4.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 11.0, Element B2: Sampling and Methods Requirements



### 37 Table 10

- 1: Sediment Sample Identifications and Station Coordinates

Sample ID Sample Type & Description Latitude ( Dec. Degrees) Longitude

( Dec. Degrees)

JAX

- Sp10

- N02

- Chem

North Candidate Site Sediment Samples 

### 30.50840910 81.3429191

JAX

- Sp10

- N03

- Chem 

### 30.49242385 81.3353077

JAX

- Sp10

- N04

- Chem 

### 30.49248986 81.3085738

JAX

- Sp10

- N06

- Chem 

### 30.47249195 81.3357043

JAX

- Sp10

- N07

- Chem 

### 30.47688254 81.3106165

JAX

- Sp10

- N08

- Chem 

### 30.46626911 81.2858312

JAX

- Sp10

- N09

- Chem 

### 30.4453156 5 81.2929732

JAX

- Sp10

- N 10

- Chem North Candidate Site Field Split TBD in Field TBD in Field

JAX

- Sp10

- S02

- Chem

South Candidate Site Sediment Samples 

### 30.36311619 81.2873387

JAX

- Sp10

- S04

- Chem 

### 30.32153037 81.2966211

JAX

- Sp10

- S05

- Chem 

### 30.32230372 81.2841042

JAX

- Sp10

- S06

- Chem 

### 30.31529371 81.2933219

JAX

- Sp10

- S07

- Chem 

### 30.28450908 81.2723900

JAX

- Sp10

- S08

- Chem 

### 30.46626911 81.2858312

JAX

- Sp10

- S13

- Chem 

### 30.35000853 81.28421537

JAX

- Sp10

- S 14

- Chem South Candidate Site Field Split TBD in Field TBD in Field



### Ta ble 10

- 2: Macroinfaunal Sample Identification s and Station Coordinates

Sample ID 1 Replicate 2

ID (3/Station) Sample Type & Description Latitude (Deg.,

Dec. Min.) Longitude

(Deg., Dec. Min.)

JAX

- [season]10

- N01

- INF A

B

C

North Candidate Site:

8 Macroinfaunal Stations 3 Replicate Samples per Station (24 total samples for this site) 

### 30.53332466 81.3283426

JAX

- [season]10

- N02

- INF A

B

C 

### 30.50840910 81.3429191

JAX

- [season]10

- N03

- INF A

B

C 

### 30.49242385 81.3353077

JAX

- [season]10

- N04

- INF A

B

C 

### 30.49248986 81.308 5738

JAX

- [season]10

- N06

- INF A

B

C 

### 30.47249195 81.3357043

JAX

- [season]10

- N07

- INF A

B

C 

### 30.47688254 81.3106165

JAX

- [season]10

- N08

- INF A

B

C 

### 30.46626911 81.2858312Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 11.0, Element B2: Sampling and Methods Requirements



### 38 Sample ID 1 Replicate 2

ID (3/Station) Sample Type & Description Latitude (Deg.,

Dec. Min.) Longitude

(Deg., Dec. Min.)

JAX

- [season]10

- N09

- INF A

B

C 

### 30.44531565 81.2929732

JAX

- [season]10

- S01

- INF A

B

C

South Candidate Site:

10 Macroinfaunal Stations 3 Replicate Samples per Station (30 total samples for this site) 

### 30.36370728 81.30309265

JAX

- [season]10

- S02

- INF A

B

C 

### 30.36311619 81.2873387

JAX

- [season]10

- S04

- INF A

B

C 

### 30.32153037 81.2966211

JAX

- [season]10

- S05

- INF A

B

C 

### 30.32230372 81.2841042

JAX

- [season]10

- S06

- INF A

B

C 

### 30.31529371 81.2933219

JAX

- [season]10

- S07

- INF A

B

C 

### 30.28450908 81.2723900

JAX

- [season]10

- S08

- INF A

B

C 

### 30.46626911 81.2858312

JAX

- [season]10

- S12

- INF A

B

C 

### 30.33336315 81.29842003

JAX

- [season]10

- S13

- INF A

B

C 

### 30.35000853 81.28421537

1 The ‘[season]’ portion of the sample ID will be filled in as ‘Sp’ for samples taken during the spring sampling event, and filled in as ‘Fa’ for samples taken during the fall sampling event. In this w ay, the samples can be easily distinguished between the two sampling events based on sample ID.

2 The replicate sample suffix wi ll be added to each sample ID . For example, the first sample collected at Station 1 of

the North Candidate Site during the sprin g sampling event will have the code ‘ JAX

- Sp10

- N01

- INF

- A ’ written or typed

on the label . This code system is intended to allow easy differentiation between samples, including those taken in spring from those taken in fall

.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 11.0, Element B2: Sampling and Methods Requirements



### 39 Table 10

- 3: Macroepifaunal Sampl e Identifications and Station Coordinates

Sample ID 1 Replicate 2

ID (2/Station) Sample Type & Description Latitude (Deg., Dec. Min.) Longitude (Deg., Dec. Min.)

JAX

- [season]10

- N02

- EPI A

B

North Candidate Site:

4 Macroepifaunal Stations (2

inside site and 2 outside site) 2 Replicate Tows per Station (8 total samples for this site) 

### 30.50840910 81.3429191

JAX

- [season]10

- N04

- EPI A

B 

### 30.49248986 81.3085738

JAX

- [season]10

- N06

- EPI A

B 

### 30.47249195 81.3357043

JAX

- [season]10

- N08

- EPI A

B 

### 30.46626911 81.2858312

JAX

- [season]10

- S02

- EPI A

B

South Candidate Site:

4 Macroepifaunal Stations (2 inside site and 2 outside site) 2 Replicate Tows per Station (8 total samples for this site) 

### 30.36311619 81.2873387

JAX

- [season]10

- S04

- EPI A

B 

### 30.32153037 81.2966211

JAX

- [se ason]10

- S05

- EPI A

B 

### 30.32230372 81.2841042

JAX

- [season]10

- S08

- EPI A

B 

### 30.46626911 81.2858312

JAX

- [season]10

- S12

- EPI A

B South Candidate Site:

1 or 2 Additional Stations to be

Trawled if Time Permits 

### 30.33336315 81.29842003

JAX

- [season]10

- S13

- EPI A

B 

### 30.35000853 81.28421537

1 The ‘[season]’ portion of the sample ID will be filled in as ‘Sp’ for samples taken during the spring sampling event, and filled in as ‘Fa’ for samples taken during the fall sampling event. In this way, the samples can be easily distinguished between the two sampling events based on sample ID.

2 The replicate sample suffix will be added to each sample ID. For example, the first sample collected at Station 1 of the North Candidate Site during the spring sampling event will have th e code ‘JAX

- Sp10

- N02

- EPI

- A ’ written or typed on the label. This code system is intended to allow easy differentiation between samples, including those taken in spring from those taken in fall.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 11.0, Element B2: Sampling and Methods Requirements



### 40 Table 10

- 4: In Situ (CTD

- profiler) and Water Quality Station Identifications and

Station Coordinates

Station ID 1 Sample Type Longitude (Deg

- Dec Min) Longitude

(Deg

- Dec Min)

JAX

- [season]10

- N02

- CTD North Candidate Site: In Situ Water Column (CTD

- profiler) has 1 Station within the Site and

2 Stations Outside the Site Boundaries 

### 30.50840910 81.3429191

JAX

- [season]10

- N05

- CTD 

### 30.48289339 81.3224904

JAX

- [season]10

- N09

- CTD 

### 30.44531565 81.2929732

JAX

- [season]10

- S02

- CTD

South Candidate Site: In Situ Water Column (CTD

- profiler) has 1 Station Within the Site and

4 Stations Outside the Site Boundaries 

### 30.36311619 81.2873387

JAX

- [season]10

- S04

- CTD 

### 30.32153037 81.2966211

JAX

- [season]10

- S08

- CTD 

### 30.27672924 81.2879808

JAX

- [season]10

- S09

- CTD 

### 30.31821932 81.2641344

JAX

- [season]10

- S10

- CTD 

### 30.31717247 81.2649542

JAX

- Fa10

- N[ xx]

- WQ AT

BT North Candidate Site: Water Quality 2 stations per site x 2 samples per station [1 sample above thermocline (‘AT’) and 1 sample below thermocline (‘BT’)] + 1

field duplicate 2 per site TBD TBD

JAX

- Fa10

- N[xx]

- WQ AT

BT TBD TBD

JAX

- Fa10

- N[xx]

- W Q (field duplicate 2 ) AT TBD in Field TBD in Field

JAX

- Fa10

- N[xx]

- WQ AT

BT North Candidate Site: Water Quality 2 stations per site x 2 samples per station [1 sample above thermocline (‘AT’) and 1 sample below thermocline (‘BT’)] + 1

field duplicate 2 per s ite TBD TBD

JAX

- Fa10

- N[xx]

- WQ AT

BT TBD TBD

JAX

- Fa10

- N[xx]

- WQ (field duplicate 2 ) AT TBD in Field TBD in Field

1 The ‘[season ]’ within each sample ID will be replaced with a two

- digit code for the season during which the sampling event took place. For example, a March cruise collecting in situ data from the North Candidate Site, station 1 will have the sample ID ‘ JAX

- Sp10

- N02

- CTD ’ . This code system is intended to allow easy differentiation between samples, including those taken in spring from those ta ken in fall.

2 A field duplicate sample will be taken from each of the two candidate sites, and will be coded in a way that disallows the laboratory from learning which sample it duplicates.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 11.0, Element B2: Sampling and Methods Requirements



### 41 Sample Analysis

Table 10

- 5 summarizes the analyte groups to be t ested for this project: a complete list of analyses and methods is shown in Section 13.3.1.

Table 10

- 5 Sample Analysis Table

Parameters Sediment Samples Water

Quality Samples Tissue

Samples Macroinfauna

Samples Macroepifauna

Samples Physical Analyses Gra in Size Y

— — — —

TOC Y

— — — — Chemistry Analyses Metals Y Y Y

— —

Pesticides Y Y Y

— —

PCB Congeners Y Y? Y

— —

PAHs Y Y Y

— — Taxonomic Analyses Gross Wt. Biomass

— — — Y Y

Taxonomic

Identification* — — — Y Y

Species Richness

— — — Y Y

S pecies Diversity

— — — Y Y

Species Abundance

— — — Y Y

Y = analysis will be performed.

— = analysis will not be performed/not required or not applicable.

* = Determination of organisms will be to the lowest practical taxonomic level, which in most cases will be to the species level.



### Field Parameters All field measurements (e.g., temperature, dissolved oxygen, salinity, turbidity, photosynthetically active radiation) will be made using a CTD

- profiler aboard the OSV Bold

. These data will be provided to A NAMAR for processing and submission in the draft and final
reports.

10.5 Classification of Measurements as Critical or Noncritical

Not applicable.

10.6 Validation of Any Nonstandard Methods

No modifications to methods are expected in this project. Any modificatio ns will be coordinated and approved by the USACE

— Jacksonville District and EPA, Region 4 prior to implementation.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 11.0, Element B2: Sampling and Methods Requirements



### 42 11.0 ELEMENT B2

– SAMPLING AND METHODS REQUIREMENTS

11.1 Describe the Sample Collection, Preparation, and Decontamination

Procedures

All sampling will be done according to published procedures. General field methodologies and procedures follow those outlined in the Green Book (USACE/EPA 1991) and procedures documented in the Florida Department of Environmental Protection Standard Operating

Procedures fo r Field Activities ( DEP

- SOP

- 001/01 ). Quality assurance and quality control procedures are based on those described in US EPA (1995). Sediment collection and storage procedures are based on those described in US EPA (2001). Epifaunal and infaunal sampling

materials and methods are based on those describ ed in Pequegnat et al. (1990).

Field Sampling Schedule It is expected that sampling wi ll be performed in a span of four days at sea during the spr ing

2010 sampling event and a similar time span during the fall 2010 sampling event . This

schedule is dependent on several factors including but not limited to weather conditions, equipment functionality, and accessibility. Contact with facilities in the immediate area and local security forces (e.g., USGC) will be coordinated prior to mobilizing to the field by EPA and USACE. Contact information for all parties involved as well as local facilities and security forces will be distributed to all parties a nd will be on

- hand in the field.

Sample Position Accuracy GPS station coordinates and sample depths will be recorded at the time of sampling by equipment and personnel aboard the OSV Bold . All parametric measurements will be provided by EPA to ANAMAR for inclusion in the draft and final reports. For sediment and macroinfaunal sampling, actual sampling locations will be within a 50 m range of the sampling stations. Water quality samples will be taken within 200 m of the sampling station. Macroepi faunal transect starting points (i.e., when the trawl first reaches the seafloor) will be within 200 m of the sampling station , and the direct ion of travel will be away from the station

.

Sediment Sampling Site conditions such as prevailing weather, wind directio n, air temperature, and tidal cycle will be documented at each sampling site. In situ measurements, water depth, date and time, coordinates, current conditions, sediment descriptions, number of containers, and team members will be recorded on project

- spec ific field sheets. Sediment samples will be photo

documented directly after collecting. A Young

- modified Van Veen grab sampler will be used to

facilitate the extraction of sediment from the seafloor. The grab sampler, and all utensils used during the se diment sampling and bagging process, will be decontaminated between each sampling station. Decontamination procedures will follow those specified in Florida Department of Environmental Protection Cleaning/Decontamination Procedures (FC 1000).

Macroinfaun al Sampling EPA, Region 4

, in coordination with A NAMAR personnel

, will collect a total of 54 macroinfaunal samples per sampling event

. Additional samples will be taken if time permits. Samples will be collected with a Young

- modified Van Veen grab with an internal volume of approximately 0.04

m 2



### . Once on

- deck, the sample will be inspected for signs of leakage from the grab sampler. If the grab sampler is determined to be less than full, the sample is discarded and anotherSampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 11.0, Element B2: Sampling and Methods Requirements



### 43 attempt is made to gather a full grab of sediment. If the grab sampler is determined to be full, then the sample will be deposited into a stainless steel bin and carefully transferred into a

standard US # 35 mesh size (0.5 mm mesh size) sieve bucket. Each sample will be gently wet

sieve d through the sieve bucket until all particles smaller than 0.5 mm have passed through the

screen. The remaining sediment and invertebrates are then gently washed into a cloth bag containing the sample label, and the bag is secured at the opening with two cable ties. Sample bags are then immediately placed into a bucket containing a fixative. Eith er undiluted

NOTOXhisto (if provided by EPA), or a 10% buffered formalin solution (supplied by ANAMAR)

will be used as a fixative in infaunal sample buckets

. B uckets will be labeled as to the samples

contained within each. The samples will be shipped to Barry A. Vittor & Associates, Inc. for sorting and analysis fol lowing completion of each sampling event

.

Macroepifaunal Sampling

Invertebrates and fishes will be collected in a 7.3 m wide otter trawl, with a liner of 14 mm stretch mesh in the b ag end, during macroepifaunal sampling in both the spring and fall 2010 sampling events . During each sampling event

, tows will be conducted in each candidate site for

a t otal of 16 tr awl samples collected per event . Each sample will consist of a 10

- minute tow at a speed of between 1.5 knots and 2 knots. This should provide a sampling area of approximately 11 ,110 m 2 

### to 11550 m 2 

### of seafloor coverage per tow, provided the t rawl doors are positioned 7.3 m apart with the lead line touching the seafloor throughout the 10

- minute

tow . Towing times may be modified somewhat if catch rates are deemed too low for adequate data gathering , or if too much biomass is contained within th e bag, hindering analysis

. Efforts will be made to tow after dark as well as during the day at each trawl station in order to more fully represent the benthic community. Spatial coordinates will be recorded at the beginning and at the end of each tow, al ong with the direction of travel, water depth, tide sequence, estimated substrate, and weather parameters. Upon completion of each tow, specimens will be carefully and thoroughly removed from the trawl net and bag , taking care not to overlook any

still co ntained in the net (to avoid cross

- contamination between samples) . Each trawl sample

will the n be weighed (wet weight) using a 5

- gallon plastic bucket with drain holes drilled in it

and either a 5

- kg or 20

- kg hanging Macro Line scale having an accuracy of within 0.3% of maximum weight . The specimens will be taxonomically identified and enumerated

, and the first 10 individual fish per species from each tow will be measured

. Fish m easurements will consist

of standard length for teleosts, precaudal length f or sharks, total length for s kates, and disk width for stingrays . Selected invertebrate species will be measured if time permits, and these measurements may include valve length in selected clam and scallop species, carapace width for selected crab specie s, and carapace length for selected shrimp species. All data will be

recorded on project

- specific epifaunal field sheets.

During the fall sampling survey, a total of 18 invertebrate and fish specimens will be selected for tissue sampling. Using decontam inated stainless steel fillet knifes and forceps, tissue samples w ill be extracted from muscle tissue (e.g., fillet of fish , tail of shrimp, claw of crab). A

10

- gram portion of tissue will be used for metal analysis, a 20

- gram portion for PCBs and

chlorin ated pesticides, and a 10

- gram portion will be taken to test for PAHs [totaling 40 grams (1.4 ounces) of tissue] . If the specimens are too small for all tissue portions, multiple specimens of the same species will be used from the same trawl sample

. A to tal of two fi eld duplicate tissue sample s will be taken for laboratory quality control purposes

. In additional to the field duplicate samples, two of the remaining tissue samples will need to weigh at least 120

-Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 11.0, Element B2: Sampling and Methods Requirements



### 44 grams each and will be used for laboratory q uality control and triplicate runs of various analytes. Tissue samples will be individually wrapped in clean aluminum foil, a label affixed on

the outside of the foil, and then bagged in gallon

- sized Ziploc ® 

### bags and quickly placed in a n onboard freezer f

or th ermal preservation at or below 0°C . The tissue samples will be kept

frozen for later delivery to the CAS laboratory.

Upon completion field sorting and identification of trawl specimens, representative specimens of

each fish species caught will be pl aced in a 5 gallon bucket and a fixative added so that there is

more fixative than biomass in each bucket. A solution of 10% buffered formalin (1 part formalin to 9 parts seawater) will be used as the fixative. Fishes that measure more than 15

- cm in leng th will be injected with 10% buffered formalin prior to being added to the bucket , in an effort to prevent internal decay . Each bucket will then be sealed using a secure

- fitting

, water

- tight lid. A separate bucket will be used per trawl sample, and a lab el will be added to

the solution and another label affixed to the outside of the bucket. Invertebrate specimens will be relaxed (if appropriate) using a chemical supplied by the Florida Museum of Natural History, Invertebrate Zoology C ollection, and place d in 50

– 70% ethanol for long

- term storage.
Invertebrate and fish preserved specimens and associated field data will later be deposited and

archived at the Florida Museum of Natural History I chthyology and I nvertebrate Z oology

collections to serve as vouch ers and to allow further study by interested researchers.

Physi c ochemical Studies of the Water Column During the spring and fall sampling events , in situ water parameters such as temperature, salinity, dissolved oxygen, turbidity, and photosynthetically a ctive radiation will be obtained throughout the water co lumn in each candidate site. Additional sampling will be conducted at stations located around the candidate sites, to be chosen while in the field based on prevailing

current direction and influence of the St. Johns River. Data will be recorded via a conductivity, temperature, and depth profiler (CTD

- profiler ) supplied by EPA. Data obtained using the CTD

profiler will be given to ANAMAR for post

- processing and to be used in a later report. Post

pro ce ssing will be facilitated with Sea

- Bird SBE data processing software. Technicians onboard

the OSV Bold are responsible for maintenance and calibration of the CTD

- profiler.

In addition to the CTD data, water quality samples will be collected from each o f the two candidate sites during the fall 2010 cruise. In each site, samples will be collected from above

the thermocline and below the thermocline near the substrate . One field duplicate sample will

also be taken at each candidate site

. Water sampling bottles, attached in a rosette within a carousel sampling unit, will be supplied by the OSV Bold

, while various pre

- cleaned collection

containers will be supplied by ANAMAR . Collection containers will include var ious pre

- cleaned glass and/or plastic bottl es. All water samples will be stored at less t han 4 ̊C on wet ice inside

coolers and/or in a refrigerated unit for thermal preservation until delivered to the CAS laboratory immediately following completion of the spring sampling event

.

Field Conditions Site conditions such as prevailing weather, wi nd direction, air temperature, and tidal cycle will be documented at each sampling site. In situ measurements, depth, date and time, coordinates, current conditions, sample descriptions, number of containers, and team members will be recorded on project

- s pecific field sheets.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 11.0, Element B2: Sampling and Methods Requirements



### 45 Decontamination All equipment contacting sediment or water samples will be cleaned and decontaminated as

described in Florida Department of Environmental Protection Cleaning/Decontamination procedures (FC 1000) . Below is a summari zed list of procedures. Work surfaces on the sampling vessel will cleaned before the sampling day begins and before leaving each station. Decontamination of all sampling handling equipment , along with the Young

- modified Van Veen grab sampler and water qu ality sampling bottles, will occur before mobilization and between sampling stations in order to prevent any contamination of samples.

Decontamination Procedures

Wash and scrub to remove gross contamination

Wash and scrub with Liquinox ®



### Rinse with tap or site water (depending on availability)

Rinse with deionized water

2X Rinse with pesticide grade isopropanol

2X Rinse with deionized water

Air dry

Storage wrapped in new, clean aluminum foil

Any derived waste will be contained and disposed of in accordance with federal, state, and local

laws.

Field Splits/Duplicates Of the sediment samples, a total of four field split samples will be collected during the spring

2010 sampling event for use in quality control and quality assurance with physical and chemical

l aboratory analyses . These field split sediment samples will be divided into 1 field split for the North Candidate Site, and one for the South Candidate Site. A total of two field duplicate macroepifaunal tissue sample s will be collected during the fall s ampling event . One water quality field duplicate sample will be collected from each candidate site during the fall sa m pling

event . The field split samples and field duplicate samples will be given unique identification

codes from that of the native in or der to ensure the samples are blind to the laboratories conducting the analyses.

Sample Homogenization Sediment samples will be homogenized in the field. Each sample will be thoroughly mixed using stainless steel spoons which have been decontaminated pr ior to use. Samples for physical and chemical testing will be transferred to appropriate sample containers and labeled with the corresponding sample identification code, date, and sampler(s) initials.

Sample Handling Prior to Shipment to Laboratories

Af ter collection, the samples will be immediately placed in properly labeled containers, and samples requiring thermal preservation will be stored in coolers and/or with in one or more cold storage rooms onboard the OSV Bold . The temperature of the cold stor age room(s) will be checked twice daily, once in the morning and once in the late afternoon or early evening

, toSampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 11.0, Element B2: Sampling and Methods Requirements



### 46 ensure compliance with EPA storage requirements. If the temperature in cold storage remains slightly above 4 ̊C (5

– 6 ̊C) for two consecutive readings, or if one reading is above 8 ̊C, wet ice will be placed on and around the samples.

Macroepifaunal tis sue samples will be stored at or below 0 ̊C (frozen) inside a freezer onboard

the OSV Bold

. Macroinfaunal samples will be stored without thermal preservation, within an aqueous solution of NOTOXhisto fixative . Representative specimens from the macroepifau nal samples will be stored in a solution of 10% buffered formalin within 5

- gallon plastic buckets.

During transport from the field to ANAMAR headquarters, all samples requiring thermal preseration will be maintained at less than 4 ̊C , with the exception of the tissue samples, which will be maintained at approximately 0 ̊C (frozen) . ANAMAR will utilize wet ice and/or a refrigerated trailer for thermal preservation of sediment and water samples . Temperatures will be checked twice daily and will follow the gu idelines shown below in Sample Storage and Transport for acceptance criteria and corrective action. Tissue samples will be stored within a freezer while at ANAMAR headquarters and prior to shipment.

Representative m acroepifaunal fish specimens will be s tored in a solution of 10% buffe red

formalin in 5

- gallon plastic buckets with tight

- fitting lids during transport to ANAMAR headquarters. Invertebrates captured during macroepifaunal surveys will be stored and transported in 50

– 70% ethanol inside 5

- gallon plastic buckets with tight

- fitting lids. Macroinfaunal samples will be stored and transported in undiluted NOTOXhisto fixative inside 5

- gallon buckets. Macroinfaunal samples will then be quickly shipped to Barry A. Vittor & Associates, Inc. Macroepifau nal samples will be stored at ANAMAR until final species determinations are made, at which time representative specimens and associated data will be deposited at the I chthyology and I nvertebrate Z oology collections at the Florida Museum of Natural History in Gainesville, Florida.

Sample Storage and Transport

Chain

- of

- custody forms will be filled out and will accompany samples at all times during transport from ANAMAR headquarters to the respective laboratory. The forms will note the sample ID and date and time of collection. Each sample will have a unique alphanumeric code assigned to it which corresponds to those written on the corresponding field sheets. The samples will be identified as specified in Tables 10

- 1 through 10

- 4. After samples will be logged in at the laboratory and assigned a unique laboratory ID number, they will be stored, handled, processed, and analyzed as described in the Quality Assurance Manuals and/or Standard Operating Procedures (SOPs) of the testing laboratories. Transport of samples from the port at Jacksonville to ANAMAR headquarters in Gainesville will be accomplished using vehicles.

11.2 Identify Support Facilities for Sampling Methods

All sampling will be conducted according to published procedures.

All sampling will be co nducted aboard the OSV Bold . A former navy ship (ex

- USNS Bold

, originally the USNS Vigorous ) , the Bold is now equipped for ocean and coastal monitoring and

has been owned an d operated by the EPA since March 2004 . This 224

- foot long vessel has a

43

- foo t b eam and a 15

- foot draft and a hull displacement of 2,300

- tons. This vessel has aSampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 11.0, Element B2: Sampling and Methods Requirements



### 47 sustained speed of 11

- knots, has an operating crew of 19, and can sleep 20 visiting scientists.

The OSV Bold is equipped for all sampling discussed in this document, includi ng the collection of sediment, water, macroinfauna, and macroepifauna samples. Onboard facilities include laboratories designed to allow processing and analyzing of samples, and refrigerated units for thermal protected sample storage (EPA 2010).

ANAMAR w ill use sampling equipment aboard the Bold for collecting samples of sediment, macroinfauna, macroepifauna, and water quality along with recording in situ water column readings. ANAMAR will provide stainless steel pans, mixers, sample containers, taxonomi c literature, cameras, scales, measuring devices, certain fixatives, labels, field sheets, and coolers for use with various samples.

Table 11

- 1: Standard Operating Procedures Used During Sampling

Standard Operating

Procedure Description Sections Reference d

FD 1000 Documentation Procedures Sections FD 1100, FD 1200, FD 2000,

FD 3000, FD 4000, FD 4100, FD 5000,

FD 5100, FD 5200

FC 1000 Cleaning/Decontamination Procedure The applicable information from this

SOP is shown in Section 11.1 of this QAPP

FS 2000 General Aqueous Sampling Sections FS 2000, FS 2001, FS 2002,

FS 2003

FS 4000 Sediment Sampling Sections 1

– 3, Table FS 4000

- 1

11.3 Describe Sampling/Measurement System Failure Response and

Corrective Action Process

Any event that does not conform to the QAPP , SOPs, or SAP is considered a nonconformance event. Any such event will be identified as quickly as possible and reported to the project manager (PM) as soon as practical. If a nonconformance event happens in the fieldwork portion of this project, it wi ll be documented in the DQCR. The project director and/or project manager will confer with EPA and USACE

— Jacksonville to outline a procedure for accomplishing the task so the quality of the project is not compromised. Every effort will be taken to contac t the USACE representative prior to any deviation from the above

- mentioned procedures.

11.4 Describe Sampling Equipment, Sample Preservation, and Holding Times

Sediment Sediment samples will be collected using a stainless steel grab sampler supplied by EPA. Once each sample is collected, it will first be placed into a decontaminated stainless steel bin. This will allow for the sediment to be categorized and photo

- documented , and allow the Contractor

to determine if there is excess water or material that shou ld be removed, such as live organisms, debris, or material which is not consistent with the overall nature of the sediment. Once the material is properly categorized and all unusable material has been removed and documented, the sediment will be placed in Teflon ® 

### bags, decontaminated glass jars, or Ziploc ®


### bags for transport.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 11.0, Element B2: Sampling and Methods Requirements



### 48 Any sampling device or material coming into contact, at any time, with a sample will first be decontaminated as described in Section 11.1 and made of an approved material (e.g., Te flon ®



### , stainless steel, glass).

Sediment samples for physical analysis shall be placed in 1

- gallon Ziploc ® 

### bags. Each sample

will be double

- bagged, and the zippered opening further secured with duct tape, to decrease the chance of loss of sample.

Macr oinfauna Samples will be collected with a Young

- modified Van Veen grab sampler with a sampling area of approxima tely 0.04 m 2 

### . The samples will be gently wet

- sieved through a sieve bucket (0.5

- mm mesh), photo

- documented, and decanted into a cloth bag conta ining the sample label. Each

cloth

- bagged sample will then be placed into a 5

- gallon plastic bucket containing u ndiluted NOTOXhisto (if provided by EPA) , or 10% buffered formalin solution (provided by ANAMAR)

. The buffered formalin solution, if used inst ead of NOTOXhisto, will consist of 9 parts seawater

and 1 part full

- strength formalin . There is no thermal requirement for these sa mples once submerged in fixative , and samples are planned to be stored at ambient temperature during the sampling event and subsequent transport to ANAMAR headquarters. The samples will be shipped to the Barry A. Vittor & Associates, Inc. for analysis following completion of each cruise. There is no holding time for these samples but ANAMAR intends to ship them to the laborat ory soon after completion of each cruise.

Macroepifauna Large epifaunal invertebrates and demersal fishes will be collected using an otter trawl having a head rope measuring 7.3

- meters. This trawl will be supplied by EPA. After each tow, the trawl

net will be brought aboard the Bold and specimens will be transferred into one or more clean

bin . Scientists will inspect the net and pick out any remaining fauna with stainless steel forceps

or nitrile

- gloved hands. Every effort will be made to remove all c aptured fauna and debris fro m the net so as to greatly reduce the chance of cross

- contamination between tow samples. Each trawl sample will then be weighed (wet weight) using a 5

- gallon plastic bucket with drain holes

and either a 5

- kg or 20

- kg hanging Ma cro Line scale with a accuracy of within 0.3% of maximum weight . In the event that significant amounts of non

- benthic invertebrates (i.e., cannonball jellyfish

, Stomolophus meleagris or ; Atlantic brief squid

, Lolliguncula brevis ) or fishes (i.e., bay ancho vy, Anchoa mitchil li ) are found within the net , the decision may be made to weigh th ese non

- benthic animals separately from the remaining sample to facilitate a better representation of the benthic community . Literature on identification and dichotomous k eys will be used and will be supplied by ANAMAR. Field sorting and identification will be conducted

on board the Bold as time allows. The first 10 individual fish per species from each tow will be measured. Fish measurements will consist of standard leng th for teleosts, precaudal length for sharks, total length for skates, and disk width for stingrays. Selected invertebrate species will be measured if time permits, and these measurements may include valve length in selected clam and scallop species, cara pace width for selected crab species, and carapace length for selected shrimp species. All data will be recorded on project

- specific epifaunal field sheets.

Upon completion of field sorting and identification, representative invertebrate and fish speci mens (at least one specimen per species) will be retained for later verification of identitySampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 11.0, Element B2: Sampling and Methods Requirements



### 49 and to provide a representative voucher series and to ensure taxonomic consistency

. Invertebrates will be preserved in a solution of ethanol. Certain invertebrat e taxa will f irst be immersed in a relaxing agent prior to preservation. Representative fish specimens will be placed in a 5

- gallon plastic bucket containing 10% buffered formalin solution (1 part full strength formalin to 9 parts seawater) as a fixative. Enough of the fixative will be added so that there is more solution than biomass. Fishes measuring more than 15

- cm standard length will be injected with 10% formalin prior to being added to the bucket in an effort to reduce the chance of internal decay. A labe l will be added to the solution, and another label will be affixed

to the outside of the bucket.

During the fall sampling event, muscle tissue samples will be obtained from selected invertebrate and fish specimens (e.g., fillet of fish, tail of sh rimp, claw of crab) using decontaminated stainless steel fillet knifes and forceps . A 10

- gram portion of tissue will be used for metal analysis, a 20

- gram portion for PCBs an d chlorinated pesticides, and 10

- gram portion for PAHs, thus a total of 40

- grams (1.4 ounces) will taken per specimen . If the specimens are t oo small to provide 40

- grams of tissue

, then multiple specimens will be used

from the same species. A total of two field duplicate tissue sample s will be taken during the fall sampling event to be used for laboratory quality control/quality assurance purposes

. In additional to the field split samples, two of the remaining tissue samples will need to weigh at

least 120

- grams each and will be used for laboratory quality control and triplicate runs of various analytes. Each tissue sample will be individually wrapped in aluminum foil, a label will

be affixed on the outside of the foil, and then bagged in a Ziploc ® 

### bag. The tissue samples will then be quickly frozen in an onboard freezer for thermal preservation at 0°C for later delivery to

the CAS laborator

y upon completion of the fall sampling event

.

Upon completion of tissue sampling, the remaining trawl sample will be pl aced in a 5 gallon pl astic bucket containing 10% buffered formalin solution (1 part full strength formalin to 9 parts seawater) a s a fixative. Enough of the fixative will be added so that there is more solution than biomass. Fishes measuring more than 15

- cm standard length will be injected with a fixative prior to being added to the bucket in an effort to reduce the chance of internal decay. A separate bucket will be used per trawl sample, and a label will be added to the solution along

with another label affixed to the outside of the bucket.

Physi c ochemical Studies of the Wate r Column In situ water parameters such as temperature, salinity, dissolved oxygen, turbidity, and photosynthetically active radiation will be obtained throughout the water column using a conductivity, temperature, and depth profiler (CTD

- profiler ) supplied by EPA. Data obtained using the CTD will be given to ANAMAR for post processing and will be used in a later report.

In addition to the CTD data, water quality samples will be collected using water sampling

bottles attached in a rosette within a carousel sampling unit supplied by the OSV Bold . The water sampling bottles will be decontaminated between each sampling station and during the mobilization process to greatly reduce the chance of contamination of samples. V arious pre

cleaned collection bottle w ill be supplied by ANAMAR, including various pre

- cleaned glass and/or plastic bottles. All water samples will be stored at less than 4 ̊C on wet ice inside coolers and/or in a refrigerated unit for thermal preservation until delivered to the CAS laboratory immediately following the completion of the spring sampling event.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 11.0, Element B2: Sampling and Methods Requirements



### 50 Table 11

- 2: Minimum Volume Requirements per Sample for Physical and Chemical Analysis

*For purposes of quality control in the laboratory, 2 sample s will each consist of a 120

- gram portion of tissue. This is three times the normal amount needed for metal and organic analysis. This is in addition to the 2 field split samples.



### Table 11

- 3: Container Requirements for Sediment Samples for Chemical and

Physical Analysis

MATRIX

- SEDIMENT

Parameters Container Type and Size Analytical Holding Time

Physical Analyses Ziploc ® bag 3.8 L Not critical

TOC Glass or

Teflon ® 120 ml 14 days

Metals Glass or

Teflon ® 120 ml 6 months for all metals except mercury; 30 days for mercury
Orga nics (pesticides, PAHs, PCBs) Glass or

Teflon ® 1 L 14 days for extraction, 40 days after extraction for analysis



### Table 11

- 4: Container Requirements for Water Samples for Chemical Analysis

MATRIX

- WATER

Parameters Container Type and Size Preservation H olding Time

Metals Teflon ® , glass,

or plastic 1.5 L Nitric Acid to pH < 2 6 months for all metals

except mercury; 14 days for mercury

Organics (pesticides and PAHs) Amber glass 4 L Cool to <4 ̊C 7 days for sample extraction, 40 days after

extraction for a nalysis



### Table 11

- 5: Container Requirements for Tissue Samples for Chemical Analysis

MATRIX

- TISSUE

Parameters Container Type and Size Preservation Holding Time

Metals Teflon ®

,

glass, plastic

, or aluminum

foil < 0.5 L Frozen Undetermined

Organics (pe sticides, PAHs, PCBs) Teflon ®

, glass

,

or aluminum < 0.5 L Frozen Undetermined Analytical Group Volume Sediment per Sample Volume Water per Sample Volume Tissue per Sample

Metals 120 ml 1.5 L 10 g wet weight*

Organics (pesticides, PAHs, PCBs) 1 L 4 L 30 g wet weight*

TOC 120 ml NA NA
Physical Analysis 1

– 2 L NA NA

Archive 500

– 1000 ml NA NASampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 11.0, Element B2: Sampling and Methods Requirements



### 51 foil



### Table 11

- 6: Container Requirements for Macroinfaunal Samples for Taxonomic

Analysis

Parameters Container Type and Size Preservation Holding Time

Benthic Macroinfauna (inver tebrates) Cloth bag

contained

within a 5

- gallon bucket Ca. 1 L Undiluted

NOTOXhisto

or 10% buffered

formalin Indefinite



### Table 11

- 7: Container Requirements for Macroepifaunal Samples for Vouchering and

Verification of Identity

Parameters Container Type and Size Preservation Holding Time

Macroepifauna

invertebrates Plastic bucket 19 L (5 gal.) 50

– 70%

E thanol

solution Indefinitely

(suitable for long

- term storage) Macroepifauna

fishes Measuring < 15 cm standard length Plastic bucket 19 L ( 5 gal.) 10% b uffered

formalin or Undiluted

NOTOXhisto Change to 7 0% ethanol

within 1 month

Larger fishes of >15 cm standard length Same as

above 19 L (5 gal.)

bucket, or bagged and placed in

freezer Inject fixative

into body cavity in

addition to

emersion in

solution

, or freeze for later preservation Change to 70% ethanol

within 1 month



### All sediment and water samples, with the exception of physical samples, should be preserved in cold storage or on wet ice to < 4 ̊C. A refrigerated trailer will be used to transport samples from the field to ANAMAR headquarters prior to shipment to laboratories.

All holding times and preservation methods will conform to USEPA guidelines in QA/QC Guidance for Sampling and Analysis of Sediments, Water, and Tissues for Dredged Material Evaluations (USEPA 1995).Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 12.0, Element B3: Sample Handling and Custody Requirements



### 52 12.0 ELEMENT B3

– SAMPLE HANDLING AND CUSTODY REQUIREMENTS

Sample Handling All sample containers will be labeled accurately and filled out entirely. The label information

will be consistent with that provided on the chain

- of

- custody form. Sample labels will include the following information:

1. Project

2. Sample Identification number and station number

3. Matrix

4. Date and time of sample collection

5. Name or initia ls of collector(s)

6. Sample preservation method used, if required

Sediment samples will be homogenized as described in Section 11.1. Section 11.1 also explains procedures pertaining to sample storage and transport. The forms listed in Table 12

- 1 are used in the field to document information regarding the sample collection and handling.

Table 12

- 1: Field Activity Forms Used to Document Sample Collection and Support

Activities

All forms will be provided in the a ppendix of the final draft of this document

.

Chain

- of

- Custody Requirements

Filename Purpose

Sediment Sampling Field Sheet Records the information about how each sample was collected, as

well as in situ measurements and site conditions. It also includes a description of the sample immediately following being brought

onboard the vessel. Water Quality Sampling Field Sheet Records the information abo ut how each sample was collected, as

well as in situ measurements and site conditions. This form also records the numbers of containers used, container type, and amount of water sampled. Infaunal Sampling Log Records information such as field conditions, description of sample, and materials and methods used in procuring it. Epifaunal (Trawl) Sampling Log Describes materials and methods of capture, waypoints, field conditions, and specimen information.

ANAMAR Chain

- of

- Custody Chain

- of

- custody form for sh ipping samples from ANAMAR to the laboratories for analysis Daily Quality Control Report The Daily Quality Control Report which provides any deviations from this QAPP, Region 4 guidelines, or any anomalous behavior of the samples. It is to be filled out o n a daily basis after sample collection or any other field activities.
Temperature Log Daily temperature log for recording sample temperature. Any deviations outside the acceptable limit will necessitate immediate corrective action to bring the samples ba ck within control.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 12.0, Element B3: Sample Handling and Custody Requirements



### 53 Chain

- of

- custody records from the field will have each sample or composite ID listed. Additional

information will include s ample date and time, analyses requested, containers shipped, relinquished by, date, and time, and receiver, date, and time. A copy of the chain of custody will be made and submitted with the final report.

Sample Shipping and Tracking ANAMAR personnel wi ll deliver samples from the field to ANAMAR’s office. Sample shipping from the field or from ANAMAR to the laboratories for analysis will use a common courier (i.e., Federal Express or Air and Surface Logistics). The laboratories will be contacted on the day of shipping to inform them of the shipment, which can then be tracked by computer using the tracking number provided, or by phone. Prior to the end of the day the samples are scheduled for delivery, the recipient will be contacted to determine if the samples arrived. If there is any delay, the courier will be contacted to determine the time the samples will be delivered and any additional corrective action that may be necessary in the event of unexpected delays (e.g.,

weather

- related or other causes) . Should the delay cause the samples to rise above the acceptable temperature for storage, USACE and EPA will be contacted and corrective actions

will then be determined.

All coolers will have custody seals attached prior to shipment. Once received, eac h custody seal will be inspected to determine if it is intact and the information recorded on the chain

- of

custody form. If the custody seal is not received intact, USACE and EPA will be contacted to determine if corrective action is required.

Intra

- an d Inter

- Laboratory Tracking Upon receipt at the laboratories, the samples will be logged into the laboratory computerized information management system and assigned a unique number for tracking through the analytical process in the lab. Any lab transfer o f samples (sediment or water) will have complete chain

- of

- custody records. All chain

- of

- custody records will be included in the final report to USACE.

Storage and Disposal of Samples The laboratories will retain all remaining unused sample volume under appropriate temperature and light conditions at least until the data generated from the samples goes through ANAMAR QA/QC and is approved as acceptable. Preferably, samples will be retained until the final report is submitted to the USACE. The storage/ar chive time will be dependent on space available at the laboratory. Approval by the USACE Project Officer will be obtained prior to disposal of any sediment, water, or tissue sample if disposal is needed before the final report is submitted. Samples will be disposed of properly according to federal, state, and local laws.

Additional information regarding sample storage is presented in Section 11.1 (Sample Storage and Transport).Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 14.0, Element B5: Quality Control Requirements



### 54 13.0 ELEMENT B4

– ANALYTICAL METHODS REQUIREMENTS

13.1 Subsampling

Not applicable

13.2 Preparation of the Samples

13.3 Analytical Methods

13.3.1 Physical and Chemical Analysis

Physical and chemical analyses will be conducted on sediment samples obtained from a marine environment. Sediment samples will be thoroughly homogenized prior to bagging and pri or to obtaining splits for the required analyses. For all sediment analyses, the concentration, reporting limit (RL), and method detection limit (MDL) will be reported on a dry weight basis. Macroepifaunal tissue sample analyses will be reported on both a wet and dry weight basis.

For grain size distributions, in addition to reporting the percentages in each size class, a graph of the cumulative frequency percentages using U.S. Army Engineering (ENG) Form 2087 (Gradation Curves) or similar form will be used.

Columbia Analytical Services, Inc. (CAS) utilizes a reductive precipitation procedure to reduce the interferences from salts for metals analysis. Organic compounds are not affected by salt

interferences.

All a nalyses shall be perform ed in a timel y manner in order to allow for retesting prior to expiration of holding times. If alternative methods or detection limits are used, approval from USACE Jacksonville District and USEPA Region 4 is required.

Tables 13

- 1 through 13

- 4 summarize analyses, m ethods and target detection limits for ph ysical and chemical parameters. Parameters in red in Tables 13

- 1 through 13

- 4 indicate

deviations from SERIM guidance

. These deviations hav e been approved by the USACE

— Jacksonville District and EPA

, Region 4. The rationale is based on available historical data from the region. Some parameters have been omitted and some have been added. Please

see footnotes for detail

.

Table 13

- 1: Analytes, Methods, and Target Reporting Limits: Sediment Physical

Analyses

Pa rameter Test Method Reporting Limit



### Grain Size ASTM

- D422 1.0 % Total Solids/Water Content ASTM

- D2216

- 80 Plumb 1998 1.0 % solids Total Organic Carbon (TOC) 9060 (SW846) 0.1% Specific Gravity 1 

### Plumb 1981 Atterberg Limits 1 

### ASTM 4318D

1 This parameter h as been omitted from analysis (listed in SERIM)

.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 14.0, Element B5: Quality Control Requirements



### 55 Table 13

- 2: Analytes, Methods, and Target Reporting Limits: Sediment

Chemistry

Test Parameters Test Method Reporting Limit (Dry Weights)
METALS/OTHERS

Arsenic 6010b/200.8 0.10 mg/kg Berylliu m 1 6010b/200.8 0.50 mg/kg

Cadmium 6010b/200.8 0.10 mg/kg

Chromium 6010b/200.8 0.10 mg/kg
Copper 6010b/200.8 0.10 mg/kg

Lead 6010b/200.8 0.10 mg/kg
Mercury 7471A 0.05 mg/kg

Nickel 6010b/200.8 0.10 mg/kg

Selenium 6010b/200.8 0.20 mg/kg

Silver 6010b/200.8 0.062 mg/kg

Zinc 6010b/200.8 0.50 mg/kg Total Organic Carbon (TOC) 9060(mod) 100 mg/kg

PESTICIDES

Aldrin 8081 1.7 g/kg

Chlordane & derivatives

Technical Chlordane 8081 8.1 g/kg (cis)

– Chlordane 8081 1.7 g/kg (trans)

– Chlordane 8081 1.7 g/kg Oxychlordane 8081 1.7 g/kg

Cis

- Nonachlor 8081 1.7 g/kg

Trans

- Nonachlor 8081 1.7 g/kg

DDT & derivatives o,p’ (2,4’)

- DDD 1 808 1 1.7 g/kg p,p’ (4,4’)

- DDD 1 80 81 1.7 g/kg o,p’ (2,4’)

- DDE 1 8081 1.7 g/kg p,p’ (4,4’)

- DDE 8081 1.7 g/kg o,p’ (2,4’)

- DDT 8081 1.7 g/kg p,p’ (4,4’)

- DDT 8081 1.7 g/kg

Dieldrin 8081 1.7 g/kg

Endosulfan & derivatives

Endosulfan I 8081 1.7 g /kg

Endosulfan II 8081 1.7 g/kg

Endrin & derivatives

Endrin 8081 1.7 g/kg Endrin aldehyde 8081 1.7 g/kg

Endrin ketone 8081 1.7 g/kg Heptachlor and derivatives Heptachlor 8081 1.7 g/kg Heptachlor epoxide 808 1 1.7 g/kg Hexachlorocyclohexane (BHC)

- BHC 8081 1.7 g/kg

- BHC 8081 1.7 g/kgSampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 14.0, Element B5: Quality Control Requirements



### 56 Table 13

- 2: Analytes, Methods, and Target Reporting Limits: Sediment

Chemistry

Test Parameters Test Method Reporting Limit (Dry Weights)

- BHC 8081 1.7 g/kg

- BHC (Lindane) 8081 1.7 g/kg Methoxychlor 8081 1.7 g/kg

Mirex 1 8081 1.7 g/kg Toxaphene 8081 167 g/kg

Semi

- Volatiles Pentachlorophenol 2 8151 Modified or 8270C SIM 100 g/kg

PCB CONGENERS

PCB

- 8 Mod 8082NOAA 1 g/kg

PCB

- 18 Mod 8082NOAA 1 g/kg

PCB

- 28 Mod 8082NOAA 1 g/kg

PCB

- 44 Mod 8082NOAA 1 g/kg

PCB

- 49 Mod 8082NOAA 1 g/kg

PCB

- 52 Mod 8082NOAA 1 g/kg

PCB

- 66 Mod 8082NOAA 1 g/kg

PCB

- 77 Mod 8082NOAA 1 g/kg

PCB

- 87 Mod 8082NOAA 1 g/kg

PCB

- 101 Mod 8082NOAA 1 g/kg

PCB

- 105 Mod 8082NOAA 1 g/kg

PCB

- 118 Mod 8082NOAA 1 g/kg

PCB

- 126 Mod 8082NOAA 1 g/kg

PCB

- 128 Mo d 8082NOAA 1 g/kg

PCB

- 138 Mod 8082NOAA 1 g/kg

PCB

- 153 Mod 8082NOAA 1 g/kg

PCB

- 156 Mod 8082NOAA 1 g/kg

PCB

- 169 Mod 8082NOAA 1 g/kg

PCB

- 170 Mod 8082NOAA 1 g/kg

PCB

- 180 Mod 8082NOAA 1 g/kg

PCB

- 183 Mod 8082NOAA 1 g/kg

PCB

- 184 Mo d 8082NOAA 1 g/kg

PCB

- 187 Mod 8082NOAA 1 g/kg

PCB

- 195 Mod 8082NOAA 1 g/kg

PCB

- 206 Mod 8082NOAA 1 g/kg

PCB

- 209 Mod 8082NOAA 1 g/kg

POLYNUCLEAR AROMATIC HYDROCARBONS Acenaphthene 8270SIM/8310 20 g/kg Acenaphthylene 8270SIM/8310 20 g/kg

Anthracene 8270SIM/8310 20 g/kg Benzo(a)anthracene 8270SIM/8310 20 g/kg Benzo(b)fluoranthene 8270SIM/8310 20 g/kg Benzo(k)fluoranthene 8270SIM/8310 20 g/kg Benzo(a)pyrene 8270SIM/8310 20 g/kgSampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 14.0, Element B5: Quality Control Requirements



### 57 Table 13

- 2: Analytes, Methods, and Target Reporting Limits: Sediment

Chemistry

Test Parameters Test Method Reporting Limit (Dry Weights) Benzo(g,h,i)perylene 8270SIM/8310 2 0 g/kg Chrysene 8270SIM/8310 20 g/kg Dibenzo(a,h)anthracene 8270SIM/8310 20 g/kg

Fluorene 8270SIM/8310 20 g/kg

Fluoranthene 8270SIM/8310 20 g/kg Indeno(1,2,3

- cd)pyrene 8270SIM/8310 20 g/kg

1

- Methylnaphthalene 8270SIM/8310 20 g/kg

2

- Methylnaphthalene 8270SIM/8310 20 g/kg Naphthalene 8270SIM/8310 20 g/kg

Phenanthrene 8270SIM/8310 20 g/kg
Pyrene 8270SIM/8310 20 g/kg Organotins Monobutyltin Krone et al, 1989 10 Dibutyltin Krone et al 10 Tributyltin Krone et al 10

1 P arameters have been added to analyte list (not listed in SERIM)

. 2 Parameters have been omitted from analysis (listed in SERIM)

.



### Table 13

- 3: Analytes, Methods, and Target Reporting Limits: Water Chemistry

Test Parameter Test Method Reporting Limit

M ETALS/OTHERS

Arsenic 6010b/200.8 1 g/L Beryllium 6010b/200.8 1 g/L

Cadmium 6010b/200.8 1 g/L

Chromium 6010b/200.8 1 g/L
Copper 6010b/200.8 1 g/L

Lead 6010b/200.8 1 g/L
Mercury 7470 0.2 g/L

Nickel 6010b/200.8 1 g/L

Se lenium 6010b/200.8 2 g/L

Silver 6010b/200.8 1 g/L

Zinc 6010b/200.8 1 g/L Total Suspended Solids 160.1 5 mg/L

PESTICIDES

Aldrin 8081 0.5 g/L

Chlordane & derivatives

Technical Chlordane 8081 0.5 g/L (cis)

– Chlordane 8 081 0.5 g/L (trans)

– Chlordane 8081 0.5 g/L Oxychlordane 8081 0.5 g/L

Cis

- Nonachlor 8081 0.5 g/L

Trans

- Nonachlor 8081 0.5 g/L

DDT & derivativesSampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 14.0, Element B5: Quality Control Requirements



### 58 Table 13

- 3: Analytes, Methods, and Target Reporting Limits: Water Chemistry

Test Parameter Test Method Reporting Limit o,p’ (2,4’)

- DDD 8081 0.05 g/L p,p’ (4,4’)

- DDD 8081 0 .05 g/L o,p’ (2,4’)

- DDE 8081 0.05 g/L p,p’ (4,4’)

- DDE 8081 0.05 g/L o,p’ (2,4’)

- DDT 8081 0.05 g/L p,p’ (4,4’)

- DDT 8081 0.05 g/L

Dieldrin 8081 0.01 g/L

Endosulfan & derivatives

Endosulfan I 8081 0.01 g/L

Endosulfan II 8081 0.01 g/L

Endrin & derivatives

Endrin 8081 0.01 g/L Endrin aldehyde 8081 0.01 g/L

Endrin ketone 8081 0.01 g/L Heptachlor & derivatives Heptachlor 8081 0.01 g/L Heptachlor epoxide 8081 0.01 g/L Hexachlorocyclohexane (BHC)

- BHC 8081 0.01 g/L

- BHC 8081 0.01 g/L

- BHC 8081 0.01 g/L

- BHC (Lindane) 8081 0.01 g/L Methoxychlor 8081 0.01 g/L

Mirex 8081 0.01 g/L Toxaphene 8081 0.2 g/L

PCB CONGENERS

PCB

- 8 Mod 8082NOAA 1 g/kg

PCB

- 18 Mod 8082NOAA 1 g/kg

PCB

- 28 Mod 8082NOAA 1 g/kg

PCB

- 44 Mod 8082NOAA 1 g/kg

PCB

- 49 Mod 8082NOAA 1 g/kg

PCB

- 52 Mod 8082NOAA 1 g/kg

PCB

- 66 Mod 8082NOAA 1 g/kg

PCB

- 77 Mod 8082NOAA 1 g/kg

PCB

- 87 Mod 8082NOAA 1 g/kg

PCB

- 101 Mod 8082NOAA 1 g/kg

PCB

- 105 Mod 8082NOAA 1 g/kg

PCB

- 118 Mod 8082NOAA 1 g/kg

PCB

- 126 Mod 8082NOAA 1 g/kg

PCB

- 128 Mod 8082NOAA 1 g/kg

PCB

- 138 Mod 8082NOAA 1 g/kg

PCB

- 153 Mod 8082NOAA 1 g/kg

PCB

- 156 Mod 8082NOAA 1 g/kg

PCB

- 169 Mod 8082NOAA 1 g/kg

PCB

- 170 Mod 8082NOAA 1 g/kgSampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 14.0, Element B5: Quality Control Requirements



### 59 Table 13

- 3: Analytes, Methods, and Target Reporting Limits: Water Chemistry

Test Parameter Test Method Reporting Limit

PCB

- 180 Mod 8082NOAA 1 g/kg

PCB

- 183 Mod 8082NOAA 1 g/kg

PCB

- 184 Mod 8082NOAA 1 g/kg

PCB

- 187 Mod 8082NOAA 1 g/kg

PCB

- 195 Mod 8082NOAA 1 g/kg

PCB

- 206 Mod 8082NOAA 1 g/kg

PCB

- 209 Mod 8082NOAA 1 g/kg

POLYNUCLEAR AROMATIC HYDROCARBONS Acenaphthene 8270SIM/8310 0.005 g/L Acenaphthylene 8270SIM/8310 0.005 g/L

Anthracene 8270SIM/8310 0.005 g/L Benzo(a)anthracene 8270SIM/8310 0.005 g/L Benzo(b)fluoranthene 8270SIM/8310 0.005 g/L Benzo(k)fluoranthene 8270SIM/8310 0.005 g/L Benzo(a)pyrene 8270SIM/8310 0.005 g/L Benzo(g,h,i)perylene 8270SIM/8310 0.005 g/L Chrysene 8270SIM/8310 0.005 g/L Dibenzo(a,h)anthracene 8270SIM/ 8310 0.005 g/L

Fluorene 8270SIM/8310 0.005 g/L

Fluoranthene 8270SIM/8310 0.005 g/L Indeno(1,2,3

- cd)pyrene 8270SIM/8310 0.005 g/L

1

- Methylnaphthalene 8270SIM/8310 0.005 g/L

2

- Methylnaphthalene 8270SIM/8310 0.005 g/L Naphthalene 8270S IM/8310 0.005 g/L

Phenanthrene 8270SIM/8310 0.005 g/L
Pyrene 8270SIM/8310 0.005 g/LSampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 14.0, Element B5: Quality Control Requirements



### 60 Table 13

- 4: Analytes, Methods, and Target Reporting Limits: Tissue Chemistry

Test Parameter Test Method Reporting Limit (Wet Weights)
METALS/OTHERS
Antimony 1 6010b/200.8 0.5 mg/kg

Arsenic 6010b/200.8 0.2 mg/kg Beryllium 1 6010b/200.8 0.5 mg/kg

Cadmium 6010b/200.8 0.1 mg/kg

Chromium 6010b/200.8 0.5 mg/kg
Copper 6010b/200.8 1 mg/kg

Lead 6010b/200.8 0.2 mg/kg
Mercury 7471 0.02 mg/ kg

Nickel 6010b/200.8 1 mg/kg

Selenium 1 6010b/200.8 0.20 mg/kg

Silver 6010b/200.8 0.062 mg/kg

Zinc 6010b/200.8 1 mg/kg

POLYNUCLEAR AROMATIC HYDROCARBONS Acenaphthene 8310/8270 SIM 20 g/kg Acenaphthylene 8310/8270 SIM 20 g/kg

Anthra cene 8310/8270 SIM 20 g/kg Benzo(a) anthracene 8310/8270 SIM 20 g/kg Benzo(b)fluoranthene 8310/8270 SIM 20 g/kg Benzo(k)fluoranthene 8310/8270 SIM 20 g/kg Benzo(a)pyrene 8310/8270 SIM 20 g/kg Benzo(g,h,i)perylene 8310/8270 SIM 20 g/kg Chrysene 8310/8270 SIM 20 g/kg Dibenzo(a,h)anthracene 8310/8270 SIM 20 g/kg

Fluorene 8310/8270 SIM 20 g/kg

Fluoranthene 8310/8270 SIM 20 g/kg Indeno(1,2,3

- cd)pyrene 8310/8270 SIM 20 g/kg

1

- Methylnaphthalene 8310/8270 SIM 20 g/kg

2

- Methylnaphthalene 8310/8270 SIM 20 g/kg Naphthalene 8310/8270 SIM 20 g/kg

Phenanthrene 8310/8270 SIM 20 g/kg
Pyrene 8310/8270 SIM 20 g/kg

PESTICIDES

Aldrin 8081 1.7 g/kg

Chlordane & derivatives

Technical Chlordane 8081 4.1 g/kg (cis)

– Chlordane 8081 1.7 g/kg (trans)

– Chlordane 8081 1.7 g/kg Oxychlordane 8081 1.7 g/kg

Cis

- Nonachlor 8081 1.7 g/kg

Trans

- Nonachlor 8081 1.7 g/kg

DDT & derivatives o,p’ (2,4’)

- DDD 1 8081 1.7 g/kgSampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 14.0, Element B5: Quality Control Requirements



### 61 Table 13

- 4: Analytes, Methods, and Target Reporting Limits: Tissue Chemistry

Test Parameter Test Method Reporting Limit (Wet Weights) p,p’ (4,4’)

- DDD 8081 1.7 g/kg o,p’ (2,4’)

- DDE 1 8081 1.7 g/kg p,p’ (4,4’)

- DDE 8081 1.7 g/kg o,p’ (2,4’)

- DDT 1 8081 1.7 g/kg p,p’ (4,4’)

- DDT 8081 1.7 g/kg

Dieldrin 8081 1.7 g/kg

Endosulfan & deriva tives

Endosulfan I 8081 1.7 g/kg

Endosulfan II 8081 1.7 g/kg

Endrin & derivatives

Endrin 808 1 1.7 g/kg Endrin aldehyde 8081 1.7 g/kg

Endrin ketone 8081 1.7 g/kg Heptachlor and derivatives Heptachlor 8081 1 .7 g/kg Heptachlor epoxide 8081 1.7 g/kg Hexachlorocyclohexane (BHC)

- BHC 8081 1.7 g/kg

- BHC 8081 1.7 g/kg

- BHC 8081 1.7 g/kg

- BHC (Lindane) 8081 1.7 g/kg Methoxychlor 8081 1.7 g/kg

Mirex 8081 1.7 g/kg Toxaphene 8081 167 g/kg

Total Chlorinated Pesticides 8081 10 g/kg

Semi

- Volatiles

Pentachlorophenol 2 8151 Modified or

8270C SIM 100 g/kg

PCB CONGENERS

PCB

- 8 8082 1 g/kg

PCB

- 18 8082 1 g/kg

PCB

- 28 8082 1 g/kg

PCB

- 44 808 2 1 g/kg

PCB

- 49 8082 1 g/kg

PCB

- 52 8082 1 g/kg

PCB

- 66 8082 1 g/kg

PCB

- 77 8082 1 g/kg

PCB

- 87 8082 1 g/kg

PCB

- 101 8082 1 g/kg

PCB

- 105 8082 1 g/kg

PCB

- 118 8082 1 g/kg

PCB

- 126 8082 1 g/kg

PCB

- 128 8082 1 g/kg

PCB

- 138 8082 1 g/kgSampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 14.0, Element B5: Quality Control Requirements



### 62 Table 13

- 4: Analytes, Methods, and Target Reporting Limits: Tissue Chemistry

Test Parameter Test Method Reporting Limit (Wet Weights)

PCB

- 153 8082 1 g/kg

PCB

- 156 8082 1 g/kg

PCB

- 169 8082 1 g/kg

PCB

- 170 8082 1 g/kg

PCB

- 180 8082 1 g/kg

PCB

- 183 8082 1 g/kg

PCB

- 184 8082 1 g/kg

PCB

- 187 8082 1 g/kg

PCB

- 195 8082 1 g/kg

PCB

- 206 8082 1 g/kg

PCB

- 209 8082 1 g/kg

1 This parameter has been added to the analyte list (not listed in SERIM)

. 2 This parameter has been omitted from the analysis (listed in SERIM)

.



### 13.3.2 Taxonomic Analysis of Benthic Macroinfauna

Biological characteriza tion of benthic macroinfauna is to be performed by Barry A. Vittor & Associates, Inc. The tasks inclu de sorting, identification, enumeration

, and wet weight

biomass of macroinvertebrate organisms collected at each station (see Barry A. Vittor &

Associates , Inc. 2004 for further details)

. The contractor shall sort and identify all infaunal invertebrates to the lowest possible identification level, which in most cases will be to the species level. The contractor will also provide analysis and interpretatio n of the results for comparison between candidate sites

.

13.3.3 Taxonomic Analysis of Benthic Macroepifauna

Characterization of trawl

- caught invertebrates and fishes will be performed by an ANAMAR biologist. Total biomass (fishes and invertebrates together) wil l be weighed (wet weight) immediately upon removal from the otter trawl. Invertebrates and fishes will be enumerated and determined to the lowest possible taxonomic level (usually to species level, unless badly damaged during collection). Crustaceans wil l be measured in carapace length or width, as appropriate, while other invertebrates will be measured based on the most widely used method for that taxa. Teleost fishes will be measured in standard length, while elasmobranch metrics will be taken using th e most widely used and accepted methods (i.e., precaudal length for sharks, disk width for non

- rajid batoids, total length for rajids). For species represented by more than 10 specimens per trawl sample, only the first 10 specimens

encountered will be mea sured, while all additional specimens will be counted and the number recorded on a field sheet. During the fall sampling event, select species of invertebrates and

fishes will be tissue sampled for analysis of certain metals, PCBs, chlorinated pesticides, and hydrocarbons. These tissue samples shall consist of muscle tissue from each taxa in question (e.g., fillet of fish, tail of shrimp, claw of crab). Tissue collection materials and methods shall

be based on those outlined in the Revised Procedural Gui de for Designation Surveys of Ocean Dredged Material Disposal Sites (April, 1990). Prior agreement by the USACE project officer must be obtained before project methods are allowed to diverge from that of the proceduralSampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 14.0, Element B5: Quality Control Requirements



### 63 guide. Tissue samples will be store d frozen and shall be shipped to laboratories soon after completion of the fall sampling event

. Representative fishes not used in tissue sampling shall be placed in a 5 gal. plastic bucket containing 10% buffered formalin solution , or undiluted

NOTOXhisto for fixation. Large fishes more than 15

- cm standard length or width shall be injected with 10 % formalin into the body cavity prior to emersion in the fixative. A label containing pertinent information shall be placed inside the bucket and another label w ill be

affixed to the outside of the bucket. All or most invertebrates collected in the trawl survey will be relaxed in a chemical supplied by the Florida Museum of Natural History Ichthyology Collection and placed in 50

– 70% ethanol for long

- term storage. All field data will be recorded on field sheets. Representative invertebrate and fish specimens will be deposited at the Invertebrate Zoology and Ichthyology collections at the Florida Museum of Natural History in Gainesville, Florida, to be used as vou chers and to allow for possible future v erification of

identification.

ANAMAR will provide, in the resulting report, the following analyses and data based on the trawl samples: Estimation of area sampled per trawl, and all trawls combined GIS figure showi ng trawl sampling locations and directions of travel Wet weight biomass per tow sample Estimation of local population density of major taxa Numbers of principle species of each taxa (e.g., gastropods, shrimps, crabs, fishes) Community composition

Taxonomi c diversity and richness of benthic macroepifauna Results and discussion of tissue analyses

14.0 ELEMENT B5

- QUALITY CONTROL REQUIREMENTS

All chemical analyses of sediment, water, and tissues will be performed by Columbia Analytical Services, Inc. (CAS). CAS is a NELAC

- certified laboratory; all analyses will be performed according to NELAC standards. The Quality Assurance Manual (QAM) and SOPs for CAS clearly specify quantitative and qualitative objectives for each analysis such as MDLs, precision,

accuracy, completeness, representativeness, and comparability. The QAM and SOPs will be strictly adhered to for all analyses completed under the project. Standard quality

- control checks including sample splits and replicates, blanks, spiked blanks, matrix spikes and duplicates, surrogate and internal standards, and calibration standards will be incorporated into all laboratory activities and described in the laboratory’s QAM and SOPs.

All physical analyses will be performed by MACTEC in Jacksonville, Florida. MA CTEC maintains laboratory SOPs and a Quality Manual as part of their overall quality assurance program. MACTEC adheres to a quality assurance program including routine analyses of proficiency tests and laboratory duplicates. Project

- specific quality contr ol may be requested if not part of the routine quality control performed.

All macroinfaunal samples analyzed by Barry A. Vittor & Associates, Inc. undergo a quality control program as part of their standard operating procedures. At least 10% of all sorte d specimens from a given sample will be re

- sorted by another qualified taxonomist. If more than

5% of the re

- sorted organisms are found to be in error in terms of identification, a qualitySampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 14.0, Element B5: Quality Control Requirements



### 64 control failure is logged and an additional sample is re

- sorted. If the second re

- sorting is found to be in error of greater than 5%, all samples previously sorted by that person are then re

sorted by another taxonomist. In addition, voucher collections are prepared for each project, composed of representative specimen s from each species encountered in that project. These voucher specimens are labeled and archived for long

- term storage and possible verification of identifications with outside specialists. Senior taxonomists (having at least 5 years experience with a p articular taxonomic group) check completed data sheets for questionable appearances of some taxa. Quality control is further accomplished by the laboratory manager, who scrutinizes each data sheet prior to submittal to the data clerk for coding. With eac h new project, the laboratory manager completes a written laboratory quality control report, with summarizes all phases of involvement. This quality control report is made available to the

client.

The QAM and SOPs for all laboratories performing analyses for this project list the analytical equipment used for testing, along with relevant calibration and standard reference materials used, maintenance schedules, and recordkeeping methods. The accuracy and precision limits included in the QAM and SOPs of th e analytical laboratory will meet the criteria established for this evaluation. The laboratory managers/directors listed in Section 4.3 will be responsible for assigning appropriately

- trained analysts to perform the specific tests. As part of the NELAC

c ertification, corrective procedures have been established if QA objectives are not met. All laboratory QAMs are provided electronically on the attached CD.

F ield analyses will be performed by ANAMAR personnel . Macroepifaunal field sorting may be

assist ed by trained individuals associated with the University of Florida under the close supervision of ANAMAR scientists . ANAMAR maintains strict quality assurance protocols in the field, including instrument calibration, decontamination, field custody sheets and corrective action, if required. All quality control is completely documented and included in the final report

to the client.

USACE

- Specific Data Quality Objectives

All physical and chemical data must conform to the data quality objectives listed in Table 7

- 1 of this document. For all data quality objectives that are not achieved, the laboratory will provide documentation for the failed criteria and any corrective action taken and/or reanalyze the samples. All analytical anomalies will be described in detail in the final report.

Data Quality Objectives for Chemical Analyses

Achieving the desired reporting limits (RLs) and method detection limits (MDLs) is critical to providing a suitable evaluation of the COCs and the suitability of this site for d isposal of

sediment. The laboratory must perform yearly MDL verification studies on the matrices tested under this project.

The final report will include detailed explanations when the actual reporting limits exceed those listed in this table and/or wh en an alternative test method is used. Any deviation from the proposed methods will receive prior approval from USACE

— Jacksonville and EPA.

Table 14

- 1 shows a list of common quality control activities that will be performed on projectSampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 14.0, Element B5: Quality Control Requirements



### 65 samples.

Table 14

- 1: Quality Control Activities Performed for Field, Physical and Chemical

Analysis

Quality Control Check Information Provided

In Situ Measurements Provides onsite conditions at the time of sample collection

Initial Calibration Ensures instrument is opera ting correctly

Initial Calibration Verification

Initial Calibration Blank Ensures instrument calibration will yield correct responses

at a known concentration. This increases confidence that sample readings within the calibration range also are read

corre ctly.

Method Blank Ensure that the preparation and analysis of the samples is not introducing potential contaminants through either the reagents used or other sources found through the laboratory. Standard Reference Material (SRM) Provides a known concen tration of contaminant that can be used to verify the preparation and analysis of samples. The SRM should be as closely matched to the sample matrix as possible. Laboratory Control Sample/Laboratory

Fortified Blank Provides a clean matrix sample which ma y be used to

determine the instrument’s calibration is within acceptance limits. Continuing Calibration Standard Continuing Calibration Blank Verifies that the initial calibration is within control, and that any drift is within acceptable limits. Surrog ates Verifies the analysis the individual contaminants performed by the analytical method. Duplicates/Triplicates Measures the precision of the analytical technique, and can be used to determine the relative homogeneity of the sample. Matrix Spike/Matrix Spike Duplicate (MS/MSD) Used to measure the analytical bias of the test, and as an indication of matrix interferences in the sample.



### ANAMAR will use the data quality review form from Appendix O of the SERIM for evaluating laboratory quality control. T hese forms correspond to the tables shown in section 7.0 of this

document.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 15.0, Element B6: Instrument/Equipment Testing, Inspection, and Maintenance Requirements



### 66 15.0 ELEMENT B6

– INSTRUMENT/EQUIPMENT TESTING, INSPECTION, AND

MAINTENANCE REQUIREMENTS

Field Instruments: The field instruments to be used for this project will be provided by the

EPA aboard the Bold

. All calibration, operation, and maintenance will be the responsibility of

Bold personnel, EPA.

Laboratory Instruments: The QAM and/or SOPs for each laboratory listed in Section 4.3 list the analytical equipment used for testing, al ong with relevant calibration and standard reference materials used, maintenance schedules, and recordkeeping methods. The accuracy and precision limits included in the QAM and SOPs of the analytical laboratory meet the criteria

established for this evalu ation. The laboratory managers/directors listed in Section 4.3 will be responsible for assigning appropriately

- trained analysts to perform the specific tests. As part of the NELAC certification, corrective procedures have also been established if QA obje ctives are

not met.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 16.0, Element B7: Instrument Calibration and Frequency



### 67 16.0 ELEMENT B7

– INSTRUMENT CALIBRATION AND FREQUENCY

Field Instruments All calibration of field instruments will be performed by EPA personnel aboard the Bold

.

Laboratory Instruments All laboratory instruments used in the analysis of sediment and site water analyses will be calibrated according to the method, laboratory Quality Assurance Manual, SOPs, or any other

NELAC

- approved method. All records of calibration will also be documented and provided in the laboratory reports according to the above procedures. Any failure of the laboratory to achieve acceptable calibration will necessitate corrective action by the laboratory in accordance with their Quality Manual and SOPs.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 17.0, Element B8: Inspection/Acceptance Requirements for Supplies and Consumables



### 68 17.0 ELEMENT B8

– INSPECTION/ACCEPTANCE REQUIREMENTS FOR SUPPL IES

AND CONSUMABLES

Pre

- cleaned glass jars or Teflon ® 

### bags will be used for sample collection of sediments for chemical analysis. Pre

- cleaned glass bottles and Nalgene ® 

### or equivalent bottles will be used

for the collection of water quality samples. Zipl oc ® 

### bags will be used for the collection of sediment samples for physical parameters. Certificates of Analysis will be kept by the laboratory according to their QAM and/or SOPs.

Equipment and supplies to be provided by personnel onboard the O SV Bold is expected to include p ositioning data (navigation and depth feed) in DAC and wet lab, Inner space 448 Depth Sounder (paper recorder not necessary), extra monitor in the wet lab, Young

- modified

Van Veen grab sampler, CTD

- profiler (sensors: conductivity, tem per ature, depth, dissolved

oxygen , turbidity, PAR), with rosette of water sampling bottles, sorti ng trays, temperature

controlled freezer, and refrigerator. Expendable Supplies from the OSV Bold are expected to

include writeable compact disk s, cable ties, ice, paper towels, de

- ionized water.

The following equipment and supplies are expected to be provided by EPA, Region 4. Data logging laptop, navigation laptop, DGPS, DGPS antennae with mounting pole, removable hard drive for data storage , sieve buckets

, cloth infaunal sample bags

, NOTOXhisto (a fixative) , grain size sample scoop, and small Young

- modified grab sampler (0.04 m 2 

### ).

Remaining supplies and equipment necessary for sampling will be supplied by ANAMAR.

All laboratory consumables will be inspecte d, handled, stored, documented, and used according to NELAC requirements and in accordance with each laboratory’s QAM and/or SOPs.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 18.0, Element B9: Data Acquisition Requirements (Non

- Direct Measurements)



### 69 18.0 ELEMENT B9

– DATA ACQUISITION REQUIREMENTS (NON

- DIRECT
MEASUREMENTS)

Various forms of data will be collected during field and laboratory activities for this project: photographs, maps, GIS data, research

- derived data, etc. All data generated during this project will be retained by the contractor. Any data not required to be submitted as described in Section 6 will be suppl ied to USACE or EPA upon request.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 19.0, Element B10: Data Management



### 70 19.0 ELEMENT B10

– DATA MANAGEMENT, INTERPRETATION, AND REDUCTION

19.1 Data Management

Each laboratory has established NELAC

- approved procedures for data management, collection, validation, reduction, and reporting. As such, the analytical results will be extensively reviewed

in

- house by the laboratories submitting the data.

Each laboratory will submit an Electronic Data Deliverable (EDD) and a hard copy data packet to ANAMAR. All data tables will be generated from the EDD an d will be cross

- checked against the hard copy data packet. When a data packet is received by the contractor, it will be reviewed by the contractor’s QA/QC Officer, with emphasis on NELAC standards. All laboratory reports received will include laboratory QC data generated in the analysis of the project samples including results of all method blanks, lab duplicates, matrix spikes, spike duplicates, reference material, surrogate spikes, standards, check standards, and calibration verifications. The analytic al results for of these QC samples will be reviewed and documented in a CQAR for each analytical data packet received. This report will be incorporated into the final data report. The CQAR consists of a checklist and a case narrative of the analytical ru ns. Any nonconformance, QC deficiency, or other problems that would impact data quality will be addressed in the CQAR. In particular, the contractor will compare data to the data quality objectives listed in Section 7.0, as well as confirm that target de tection limits listed in Section 13.3.1 were achieved. If any data quality objective is not achieved, the laboratory will re

- analyze the sample(s) and/or provide documentation for the failed criteria. The CQAR will contain a written record of the

validit y of each data package and its subsequent use in the report.

Field parameters, sample descriptions, site conditions, and additional information pertaining to the sample and sampling process will be recorded on site

- specific field sheets. A DQCR will be

f illed out for each day of sampling and sample processing. Each of these records is integral to the successful completion of this project. As such, they will be reviewed, reported, and retained

as described elsewhere in this document.

The contractor will use the password

- protected Client Login section on the company’s website to upload documents for client access and to keep USACE

— Jacksonville up

- to

- date with all documents and project

- related data. This provides access to current documents and allows

USA CE

— Jacksonville to perform an in

- progress data review.

19.2 Data Interpretation and Reduction

Macroinfaunal Data Analysis and Interpretation The contractor shall analyze the macroinfaunal data using approaches designed to identify any differences in community structure between study areas.

The contractor shall calculate the following numerical indices for each sample:

Infaunal abundance (total number of individuals per station) Infaunal density (total number of individuals per square meter) Species richness ( total number of taxa represented per station) Taxonomic diversity (distribution of individuals over species)Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 19.0, Element B10: Data Management



### 71 The contractor also shall perform data interpretation consisting of habitat characterization and benthic community characterization including faun al composition, abundance, and community structure. The contractor preparing the final report shall also include as an appendix the phylogenetic list of macroinvertebrate taxa for the surveys.

Chemical and Physical Data Reduction of chemical and physical data in the final report will be done as discussed in the

Green Book and the SERIM.

All reports will undergo extensive internal review before submission to USACE

— Jacksonville. A compact disk will accompany the final report and will contain all of the project files including electronic versions of all data reports, maps, figures, tables, text, photos, and any other electronic files used to generate the report.

Macroepifaunal Data Analysis The contractor will provide, in the resulting report, the foll owing analyses and data based on the trawl samples:

Esti mation of area sampled per tow , and all tows combined GIS figure showing trawl sampling locations and directions of travel Numbers of principle species of each taxa (e.g., gastropods, shrimps, crabs, fishes) Wet weight biomass Estimation of local population density of major taxa

An estimation of taxonomic richness An estimation of taxonomic diversity (using an appropriate index of diversity) Community composition

Discussion of species of federal manag ement interest found in sites Results and discussion of tissue analyses

The contractor also shall perform data interpretation consisting of a brief habitat characterization and benthic community characterization. The contractor’s discussion shall

include a comparison of results obtained and reported in the 1986 New Fernandina Harbor ODMDS EIS. The contractor preparing the final report shall also include as an appendix a taxonomic list of all invertebrate and fish species collected during these surveys.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 20.0, Element C1: Assessments and Response Actions



### 72 

## GROUP C. ASSESSMENT AND OVERSIGHT

20.0 ELEMENT C1

– ASSESSMENTS AND RESPONSE ACTIONS

ANAMAR’s QA/QC Officer is responsible for all assessments and response actions throughout the life of this project and is performed in

- part through the review and audit proce ss.

Performance and systems audits are performed to evaluate the capability and performance of a measurement system. Audits are utilized to ensure that field and laboratory activities will provide data reflective of site conditions and within project Q A/QC requirements. A performance audit is used to evaluate the accuracy of a measurement method or component of the method. A systems audit focuses on evaluating the principal components of a sample collection or data collection method to determine prope r selection and use of that method.

ANAMAR’s field sampling teams will be overseen and directed at all times by the Project Manager. Field teams are audited periodically, usually annually, by the Contractor Project Director, company President, or QA/QC Officer. These audits entail an observation and critique of sampling methods, collection, preservation, labeling, handling of sediment and/or water samples to ensure SOPs are being followed, that all equipment is calibrated and used properly,

the Health a nd Safety Plan is being followed, and all aspects of the project are on schedule. Documentation of these audits is retained by Contractor.

Each laboratory used in this project regularly undergoes audits in accordance with their NELAC

certifications. A lthough no project

- specific laboratory audits are scheduled for this project, any deficiencies identified in a recent audit that may adversely affect the data quality for this project will be brought to the attention of the ANAMAR Project Manager.

Any eve nt that does not conform to the SAP/QAP, SOPs, or QAMs is considered a noncon

formance event. These will be identified as quickly as possible and reported to the Project Manager as soon as practical. If the nonconformance event happens in the fieldwork p ortion of this project, it will be documented in the DQCR. The Project Manager will confer with the

USACE

— Jacksonville Project Officer or QA/QC Officer and outline a procedure for accomplishing the task so the quality of the project is not compromised. E very effort will be taken to contact the USACE and/or EPA representative prior to any deviation from the procedures documented in this SAP/QAPP.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 21.0, Element C2: Reports to Management



### 73 21.0 ELEMENT C2

– REPORTS TO MANAGEMENT

Each laboratory shall include as part of its Quality Assurance Program pe riodic reports to management. These reports will include internal audits with corrective actions, findings with corrective actions of any external audits, either by regulatory agencies or clients, results of proficiency testing samples, and any routine is sues directly related to quality assurance or control within the laboratory that has been found since the most recent audit. These reports should be maintained as part of the permanent record of the laboratory for a minimum of five

years.

Any report which may have a direct impact on the samples included in this project should be addressed with the ANAMAR Project Manager or QA/QC Officer in order to determine the appropriate corrective action. All correspondence which may have an impact on the overall qual ity of the results will be included in the final report to the client.

Once received from the laboratories, the results will be incorporated into text, tables, and charts to more readily allow for evaluation by USACE and EPA. These documents will be avai lable to ANAMAR’s management for technical review before delivery to USACE

— Jacksonville District

.

Macroinfauna Report

Table 21

- 1 Minimum Report Requirements

1. Introduction including summary of project background and study purpose

2. Methods includin g sample collection and handling and laboratory macroinfaunal sample analysis

3. Data analysis methods including assemblage analyses and faunal similarities

4. Discussion of habitat characteristics (e.g., grain size, water depth)

5. Faunal composition, abundance, and community structure

6. Numerical classification analysis

7. Tabular summary of macroinfauna by taxonomic groups

8. Comparisons of stations within the ODMDS to those outside the ODMDS

9. Comparisons to previous surveys

10. Brief discussio n of quality assurance/quality control steps

11. Results discussion

12. Summary and conclusions.

In addition, the contractor shall provide raw data in electronic (digital) format. Two raw data files shall be provided by the contractor in accordance wi th Table 21. 1 and shall be provided in Microsoft Excel, Extensible Markup Language (XML), or comma delineated format. The contractor shall also provide statistical worksheet and output files.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 21.0, Element C2: Reports to Management



### 74 Table 21

- 2 Electronic Raw Data File Format

File Description C ontent/format

Abundance Data For each station and taxon collected, provide the following: station,

phylum, class, family, genus, species, Number collected, and abundance

Biomass Data For each station and major taxonomic group, provide the following:

stat ion, major taxonomic group, abundance.

Abundance

Statistical

Worksheet The format should have all sample sites as column headers and all species

as row headers with the number of individuals of that species for that site in the cell (including stations wh ere a given taxon was not observed).

Biomass

Statistical

Worksheet The format should have all sample sites as column headers and all species

as row headers with the biomass of a given species and a given site in the cell (including stations where a given taxon was not observed).Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 22.0, Element D1: Data Review, Validation, and Verification Requirements



### 75 

## GROUP D. DATA VALIDATION AND USABILITY

22.0 ELEMENT D1

– DATA REVIEW, VALIDATION, AND VERIFICATION

REQUIREMENTS

Data Validation Data validation is a process used to accept or reject data and determine if the data are traceable, de fensible, and can be used for a particular project. Each laboratory has established

state

- approved procedures for data collection, validation, reduction, and reporting. As such, the analytical results will be extensively reviewed in

- house by the laborato ries submitting the data.

Section 7.0 contains tables showing the acceptance criteria for the analytical groups to be performed for this project. All physical and chemical results shall be compared to the reporting limits in Section 13.3.1. All QC res ults for physical and chemical analyses will be compared to

the tables in Section 7.0.

ANAMAR’s QA/QC Officer will review all laboratory results in accordance with the procedures described above. Any data which does not meet the acceptance criteria in the data tables will be qualified in bold italics with a shaded background. After review, ANAMAR’s QA/QC Officer, Project Manager and project director will perform an overall project review and recommend whether to accept or reject the data. EPA, Region 4 and USACE

— Jacksonville District will have the final authority to determine the acceptability of the project data.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 23.0, E lement D2: Validation and Verifications Methods



### 76 23.0 ELEMENT D2

– VALIDATION AND VERIFICATIONS METHODS

Laboratory Data Validation When a physical or chemical data packet is received, it is r eviewed by ANAMAR’s QA/QC Officer, with emphasis on NELAC standards and conformance to the data quality objectives set forth in this QAPP. Chemistry data reports include laboratory QC data generated during analysis of the project samples including results of all method blanks, lab duplicates/triplicates, matrix spikes, spike duplicates/triplicates, reference material, surrogate spikes, standards, check standards, and calibration verifications. All physical testing reports will include sample duplicates/tr iplicates. The analytical results for these QC samples are reviewed and documented in a CQAR for each analytical data packet received. This report is then incorporated into the final data report. The CQAR consists of a checklist and a case narrative of the analytical runs. Any non conformance, QC deficiency, or other problem that would impact data quality will be addressed in the CQAR. ANAMAR will compare data to the data quality objectives listed in Section 7.0, as well as confirm that target detectio n limits listed in Section 13.3 were reached. If any data quality objective is not reached, the laboratory will re

- analyze the sample(s) and/or provide documentation for the failed criteria. The CQAR will provide a written record of the validity of each data package and its subsequent use in the report. After all data have been reviewed and validated, an assessment of the impact on the data quality and usability of the results will be made and included in the report to the client.Sampling and Analysis Plan for Jacksonville ODMDS Site Designation Studies

Section 24.0, Element D3: Reconciliation with Data Quality Objectives



### 77 24.0 ELEMENT D3

– RECONC ILIATION WITH DATA QUALITY OBJECTIVES

All chemical and physical data will be reconciled by ANAMAR’s QA/QC Officer with the data quality objectives listed in Section 7.0 and with the target detection limits listed in Section 13.3 by comparison with the lab oratory results. Analytical data that fall outside of the acceptable QC limits will be addressed in a case narrative and any corrective action taken will be described in detail. Quality Control failures which are not adequately addressed will be re

- prepa red or reanalyzed at the laboratory’s expense. All analytical anomalies will be described in detail in the final report. In the case of reruns, the initial and rerun result will be presented in the final
report.

Many analytical methods describe procedur es for analytical anomalies that occur during analysis. These method

- specific procedures must be followed.

### 25.0 REFERENCES

Barry A. Vittor & Associates, Inc. 2004. Draft Quality Assurance Project Plan for Benthic Macroinvertebrate and Sediment Sample Analysis and Data Interpretation for Ocean Dredged Material Disposal Sites

. Report submitted 26 April 2004 to USACE, Region 4.

Buchman, M.F. 2008. NOAA Screening Quick Reference Tables. NOAA OR&R Report 08

- 1, Seattle, WA, Office of Response and Restora tion Division, National Oceanic and Atmospheric Administration. 34 pages.

Pequegnat, W.E., B.J. Gallaway, and T.D. Wright. 1990. Revised Procedural Guide for Designation Surveys of Ocean Dredged Material Disposal Sites . Department of the Army, Waterways Experiment Station, Corps of Engineers, Vicksburg, MS.

USEPA. 1995. QA/QC Guidance for Sampling and Analysis of Sediments, Water, and Tissues for Dredged Material Evaluations

– Chemical Evaluations

. EPA

- 823

- B

- 95

- 001. Accessed

online at: www.epa.gov/waterscienc e/library/sediment/evaluationguide.pdf

.

USEPA. 2001. Methods for Collection, Storage, and Manipulation of Sediments for Chemical and Toxicological Analyses

. EPA

- 823

- B

- 01

- 002. October, 2001. Accessed online at: http://www.epa.gov/waterscience/cs/libr ary/collectionmanual.pdf

.

USEPA. 2002. Guidance for Quality Assurance Project Plans (G

- 5) [G

- 5 publication] (PDF 401KB)

- December 2002, EPA/240/R

- 02/009. (Note: This document replaces EPA/600/R

- 98/018 issued in February 1998.) Accessed online at: www.epa.gov/quality/qs

- docs/g5

- final.pdf

.

USEPA. 2006. National Recommended Water Quality Criteria: 2006. November. EPA

- 822

- R

02

- 047. Accessed online at: www.epa.gov/waterscience/criteria/nrwgc

- 2006.pdf

.

USEPA. 2010. Ocean Surv ey Vessel (OSV) Bold . A cc essed online at: www.epa.gov/bold/brochure.html.

USEPA and USACE. 1991. Evaluation of Dredged Material Proposed for Ocean Disposal

- Testing Manual ( Green Book ). EPA

- 503/8

- 91

- 001. February 1991. Accessed online at: www.epa.gov/owow/oceans/gbook/gbook.pdf

.

USEPA and USACE. 2008. Southeast Regional Implementation Manual (SERIM) for Requirements and Procedures for Evaluation of the Ocean Disposal of Dredged Material

in Southeastern U.S. Atlantic and Gulf Coast Waters . U.S. Environmental Protection Agency Region 4 and U.S. Army Corp s of Engineers, South Atlantic Division, Atlanta, GA.

Accessed o nline at: http://www.epa.gov/region4/water/oceans/documents/SERIM_Final_August 2008.pdf

.Appendix

79 

# Appendix

Sediment Sampling Field Sheet

Water Quality Sampling Field Sheet

Macroinfaunal Sampling Log

Macroepifaunal (Trawl) Sampling Log

Chain

- of

- Custody Form

Daily Quality Control Report

Temperature LogAppendix

80Appendix

81Appendix

82Appendix

83Appendix

84Appendix

85Appendix

86