# Endpoint study record: Read y Biodegradability (ISO 10707, 1994) - Vertellus 2012

Administrative Data

Data source Reference

Data access
Data protection claimed


## Materials and methods Test type
Test guideline

GLP compliance


### Test materials Identity of test material same as for substance defined in section 1 (if not read-across)
Test material identity UUID IUC5-0753c985-8190-417b-bf30-1b45f92f71ba Dossier UUID 0 Author mbogle / Vertellus Specialties Inc. Date 2012-05-31 11:03:14 EDT Remarks

Purpose flag key study; robust study summary; used for classification; used for MSDS Study result type experimental result Study period 29 September 2011 to 27 October 2011 Reliability 1 (reliable without restriction)

Reference type Author Year Title Bibliographic source Testing laboratory Report no. Owner company Company study no. Report date study report Falk, Silke 2012 Assessment of the Ready Biodegradability of Oxidized Castor Oil with the Closed Bottle Test Eurofins Agroscience Services S11- 03332 Vertellus Specialties Inc.

data submitter is data owner

yes, but willing to share

ready biodegradability

Qualifier Guideline Deviations according to other guideline: ISO 10707 (1994) yes (Temperature decreased during test period to 19.8°C; no impact on study results.)

yes

yes 

### Page 1 of 4 Endpoint study record: Ready Biodegradability (ISO 10707, 1994) - Vertellus 2012

5/31/2012 file://G:\TSCA\Test Rule - Ox idized Castor Oil\Robust Stud y Summaries\Read y Biode g .ht

...Details on test material



### Study design Oxygen conditions
Inoculum or test system
Duration of test (contact time)

Initial test substance concentration

Any other information on materials and methods incl. tables



## Results and discussions % Degradation of test substance



### BOD5 / COD results Any other information on results incl. tables Identifier Identity CAS name Castor Oil, Oxidized CAS number 68187-84-8

- Name of test material (as cited in study report): Oxidized Castor Oil - Purity test date: 15 July 2011 (date of Certificate of Analysis) - Lot/batch No.: 0000113649 - Expiration date of the lot/batch: 30 June 2012 - Storage condition of test material: Ambient - Other: Chemical Oxygen Demand (COD): 2220 mg O2/g

aerobic

activated sludge, industrial, non-adapted

28 d

Initial conc. Based on 2 mg/L COD

Source of inoculum: municipal activated sludge plant of Pforzheim, Germany Incubation Temperature: 19.8 - 21.4°C Analysis: Oxygen concentrations measures with a microprocessor oximeter and a calibrated electrode.

%Degr. St. dev. Parame ter Sampling time Remarks 8.56 O2 consumption 4 d
17.72 O2 consumption 7 d
36.64 O2 consumption 14 d
36.79 O2 consumption 21 d
38.74 O2 consumption 28 d



### Percent degradation of test item, reference item and toxicity control: 

### Page 2 of 4 Endpoint study record: Ready Biodegrad ability (ISO 10707, 1994) - Vertellus 2012

5/31/2012 file://G:\TSCA\Test Rule - Ox idized Castor Oil\Robust Stud y Summaries\Read y Biode g .ht

...

## Overall remarks, attachments Overall remarks

Attached full study report


## Applicant's summary and conclusion Validity criteria fulfilled Time (days) % degradation based on solvent control Test item (Oxidized Castor Oil) Reference item (Sodium Benzoate) Toxicity control (OCO and Sodium Benzoate) 4 8.56 63.48 20.06 7 17.72 75.10 33.90 11 21.17 81.74 36.25 14 36.64 84.20 42.00 21 36.79 85.20 52.40 28 38.74 85.00 57.80

Conclusions: The biodegradation of Oxidized Castor Oil was determined with the Closed Bottle Test according to ISO Guideline 10707 (1994). Oxidized Castor Oil was tested at a nominal concentr ation of 2 mg/L. The evaluation was based on the solvent control. The following biodegradation was determined at the end of the 28 -d period: - Oxidized Castor Oil 2 mg/L: 38.74% - Sodium Benzoate 2 mg/L: 85.00%
- Toxicity control: 57.80% The criterion for biodegradability of 60% removal of the CO D/ThOD within a 14 -day window has not been reached. Therefore, Oxidized Castor Oil cannot be considered to be re adily biodegradable under the conditions of this closed bottle test. The results of the test can be regarded as valid, as - The percent degradation of the reference item reached > 60% (84.20%) by day 14. - The residual O2 content did not fall below 0.5 mg/L at any time. - In the control, there was < 1.5 mg/L O2 consumption after 28 days (mg O2/L on average). The degradation of the toxicity control was > 25% (42.00%) after 14 days (calculated for the mixture "Sodium Benzoate, 2 mg/L + Oxidized Castor Oil, 2 mg/L "). Toxic effects of Oxidized Castor Oil, therefore, can be excluded.

Attached full study report Oxidized Castor Oil - Biodegradation (Eurof ins 2012).pdf / 1.58 MB (application/pdf)

yes 

### Page 3 of 4 Endpoint study record: Ready Biodegrad ability (ISO 10707, 1994) - Vertellus 2012

5/31/2012 file://G:\TSCA\Test Rule - Ox idized Castor Oil\Robust Stud y Summaries\Read y Biode g .ht

...Interpretation of results

Conclusions other: Not ready biodegradable

The following biodegradation was determined at the end of the 28 -d period: - Oxidized Castor Oil 2 mg/L: 38.74% - Sodium Benzoate 2 mg/L: 85.00% - Toxicity control: 57.80% The criterion for biodegradability of 60% removal of the CO D/ThOD within a 14 -day window has not been reached. Therefore, Oxidized Castor Oil cannot be considered to be re adily biodegradable under the conditions of this closed bottle test. 

### Page 4 of 4 Endpoint study record: Ready Biodegrad ability (ISO 10707, 1994) - Vertellus 2012

5/31/2012 file://G:\TSCA\Test Rule - Ox idized Castor Oil\Robust Stud y Summaries\Read y Biode g .ht

...