Endpoint study record: Ready Biodegradability (ISO 

10707, 1994) - Vertellus 2012 

Administrative Data 

Data source 

Reference 

Data access 

Data protection claimed 

Materials and methods 

Test type 

Test guideline 

GLP compliance 

Test materials 

Identity of test material same as for substance defined in section 1 (if not read-across) 

Test material identity 

UUID IUC5-0753c985-8190-417b-bf30-1b45f92f71ba 

Dossier UUID 0 

Author mbogle / Vertellus Specialties Inc.

Date 2012-05-31 11:03:14 EDT 

Remarks 

 

Purpose flag key study; robust study summary; used for classification; used for MSDS 

Study result type experimental result Study period 29 September 2011 to 27 October 2011 

Reliability 1 (reliable without restriction) 

Reference 

type 

Author Year Title Bibliographic 

source 

Testing 

laboratory 

Report 

no. 

Owner 

company 

Company 

study no. 

Report 

date 

study 

report 

Falk, 

Silke 

2012 Assessment of 

the Ready 

Biodegradability 

of Oxidized 

Castor Oil with 

the Closed 

Bottle Test 

 Eurofins 

Agroscience 

Services 

S11-

03332 

Vertellus 

Specialties 

Inc. 

 

data submitter is data owner 

yes, but willing to share 

ready biodegradability 

Qualifier Guideline Deviations 

according 

to 

other guideline: ISO 10707 

(1994) 

yes (Temperature decreased during test period to 19.8°C; no impact on 

study results.) 

yes 

yes 

Endpoint study record: Ready Biodegradability (ISO 10707, 1994) - Vertellus 2012 Page 1 of 4

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Details on test material 

Study design 

Oxygen conditions 

Inoculum or test system 

Duration of test (contact time) 

Initial test substance concentration 

Any other information on materials and methods incl. tables 

Results and discussions 

% Degradation of test substance 

BOD5 / COD results 

Any other information on results incl. tables 

Identifier Identity 

CAS name Castor Oil, Oxidized 

CAS number 68187-84-8 

- Name of test material (as cited in study report): Oxidized Castor Oil

- Purity test date: 15 July 2011 (date of Certificate of Analysis) 

- Lot/batch No.: 0000113649 

- Expiration date of the lot/batch: 30 June 2012 

- Storage condition of test material: Ambient 

- Other: Chemical Oxygen Demand (COD): 2220 mg O2/g 

aerobic 

activated sludge, industrial, non-adapted 

28 d 

Initial conc. Based on 

2 mg/L COD 

Source of inoculum: municipal activated sludge plant of Pforzheim, Germany

Incubation Temperature: 19.8 - 21.4°C

Analysis: Oxygen concentrations measures with a microprocessor oximeter and a calibrated electrode.

%Degr. St. dev. Parameter Sampling time Remarks 

8.56 O2 consumption 4 d 

17.72 O2 consumption 7 d 

36.64 O2 consumption 14 d 

36.79 O2 consumption 21 d 

38.74 O2 consumption 28 d 

Percent degradation of test item, reference item and toxicity control:

Endpoint study record: Ready Biodegradability (ISO 10707, 1994) - Vertellus 2012 Page 2 of 4

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Overall remarks, attachments 

Overall remarks 

Attached full study report 

Applicant's summary and conclusion 

Validity criteria fulfilled 

 

Time (days)

% degradation based on solvent control

Test item (Oxidized

Castor Oil)

Reference item

(Sodium Benzoate)

Toxicity control (OCO

and Sodium Benzoate)

4 8.56 63.48 20.06

7 17.72 75.10 33.90

11 21.17 81.74 36.25

14 36.64 84.20 42.00

21 36.79 85.20 52.40

28 38.74 85.00 57.80

Conclusions:

The biodegradation of Oxidized Castor Oil was determined with the Closed Bottle Test according to ISO Guideline 10707 

(1994). Oxidized Castor Oil was tested at a nominal concentration of 2 mg/L. The evaluation was based on the solvent 

control.

The following biodegradation was determined at the end of the 28 -d period:

- Oxidized Castor Oil 2 mg/L: 38.74%

- Sodium Benzoate 2 mg/L: 85.00%

- Toxicity control: 57.80%

The criterion for biodegradability of 60% removal of the COD/ThOD within a 14 -day window has not been reached. 

Therefore, Oxidized Castor Oil cannot be considered to be readily biodegradable under the conditions of this closed 

bottle test.

The results of the test can be regarded as valid, as

- The percent degradation of the reference item reached > 60% (84.20%) by day 14.

- The residual O2 content did not fall below 0.5 mg/L at any time.

- In the control, there was < 1.5 mg/L O2 consumption after 28 days (mg O2/L on average).

The degradation of the toxicity control was > 25% (42.00%) after 14 days (calculated for the mixture "Sodium Benzoate, 

2 mg/L + Oxidized Castor Oil, 2 mg/L"). Toxic effects of Oxidized Castor Oil, therefore, can be excluded.

Attached full study report 

Oxidized Castor Oil - Biodegradation (Eurofins 2012).pdf / 1.58 MB (application/pdf) 

yes 

Endpoint study record: Ready Biodegradability (ISO 10707, 1994) - Vertellus 2012 Page 3 of 4

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Interpretation of results 

Conclusions 

other: Not ready biodegradable 

The following biodegradation was determined at the end of the 28 -d period: 

- Oxidized Castor Oil 2 mg/L: 38.74% 

- Sodium Benzoate 2 mg/L: 85.00% 

- Toxicity control: 57.80% 

The criterion for biodegradability of 60% removal of the COD/ThOD within a 14 -day window has not been reached. 

Therefore, Oxidized Castor Oil cannot be considered to be readily biodegradable under the conditions of this closed 

bottle test. 

Endpoint study record: Ready Biodegradability (ISO 10707, 1994) - Vertellus 2012 Page 4 of 4

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