U.S. ENVIRONMENTAL RESEARCH LABORATORY 

DUI.ff~'kl, MN 
PB93-754672 

DRAFT 

3-3-93 

COMPARISON OF PROPOSED HHBAFs FOR THE GLI 

Charles E. Stephan 

Environmental Research Laboratory 

Office of Research and Development 

U.S. Environmental Protection Agency 

Duluth, MN 

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Standard Form 298 Back (Rev. 2-89) -I, 
DERIVATION OF PROPOSED HUMAN HEALTH AND WILDLIFE 

BIOACCUMULATION FACTORS FOR THE 

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Environmental Research Laboratory 

Office of Research and Development 

U.S. Environmental Protection Agency 

Duluth, MN 
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DRAFT 

3-3-93 

DERIVATION OF PROPOSED HUMAN HEALTH AND WILDLIFE 

BIOACCUMtlLATION FACTORS FOR THE GREAT LAKES INITIATIVE 

by 

Charles E. Stephan 

Environmental Research Laboratory 

Office of Research and Development 

U.S. Environmental Protection Agency 

Duluth, MN 
The proposed Human Health Bioaccmulation Factors (HHBAFs) and 

Wildlife Bioaccumulation Factors (WLBAFs) were derived using a 

draft of the 'Procedure to Determine a Bioaccumulation Factor' that 

was developed under the Great Lakes Initiative (GLI). (Although 

proposed HHBAFs were derived for many chemicals, proposed WLBAFs 

were derived only for DDT, mercury, PCBs, and 2,3,7,8-TCDD.) The 

proposed HHBAFs were derived to apply to fish that contain 5 .O% 

lipids and are at trophic level 4 in the Great Lakes. The proposed 

WLBAFs were derived to apply to fish that contain 7.9% lipids and 

are at trophic levels 3 and 4 in ,the Great Lakes. The number of 

digits given implies nothing about how many are significant. ~ For 

organic chemicals, predicted BCFs were calculated from Log P using 

the equation 'Log BCF = 0.79(Log P) - 0.40. (Veith and Kosian 

1983). The predicted BCFs are assumed to apply to fish that 

contain 7.6% lipids. Because chemicals.with very high values for 

Log P'do not accumulate according to the equation, the predicted 

BCF was not allowed to be greater than 100,000 at 7;6% lipids. All 

logarithms are to base 10. The 'MedChem'Star' and 'MedChem Calc. 

values for Log P were obtained from the QSAR system at ERG-Duluth 

and are based on version 3.53 of MedChem, which was obtained from 

Pomona College. Professional judgement was used when selecting a 

'typical Log P' on the basis of the various measured and predicted 

values that had been found for the chemical. Values for the Food 

Chain Multiplier (FCM) were obtained by rounding the 'typical Log 

P' to two significant digits and then using the value specified in 

the FCM table for the trophic level of concern; interpolation 

between values in the FCM table was not used. When Log P is 

greater than 6.5 and no chemical specific information concerning 

BAFs is available, a value of. 1 .O was used for the FCM as specified 

in the GLI BAF methodology. Data for freshwater species were given 

preference over data for saltwater species, especially for metals, 

metalloids, andsuch ionizable organic chemicals as 

pentachlorophenol. HHBAFs for metals and metalloids are to apply 

to the wet weight of the edible portion, e.g., muscle, of 

freshwater fish; WLBAFs for metals and metalloids are to apply to 

the wet wei.ght of the whole body of freshwater fish. If a BAF 

could be calculated for a chemical from a field study in the Great 

Lakes, BAFs from other bodies of water were not considered for tha,t 

chemical. For organic chemicals, a measured BCF or BAF was used 

only if an acceptable measurement of percent lipids was available 

so that the BCF or BAF could be normalized to 1% lipids. No 

adjustment was made to account for the different solvents that were 

used in the measurement of percent lipids. 

/ 

1 
I 

ACENAPHTHENE [CAS#: 83-32-91 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

3.92 MedChem Star 

4.07 MedChen Calc 

3.92 Miller et al. 1985 

3.97 Isnard and Lambert 1989 

A Log P of 3.84 has been proposed for use in the derivation 

of sediment quality criteria for acenaphthene (U.S. EPA 

1991a). A value of 3.98 was selected as a 'typical Log P', 

from which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 554.9' 

Normalized BCF (at 1.0% lipids) = 73.0 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHEAF that 

is calculated from the 'typical Log P' is (73.0) (5.0) (1.0) = 

365. 

> Predicted BAF based on Log P and measured BCF: 

BCF % L Norm BCF' Reference 

.(1.0% L) 

3 87 4.8 80.6 Barrows et al. 1980 

Geometric mean normalized BCF = 80.6 

Note: This BCF was based on uptake of radioactivity with no , verification of the parent chemical. Thus this BCF and 

the resulting predicted HHBAF might be too high. 

> No measured BAF was found. 

ACENAPHTHYLENE [CAS#: 208-96-81 

> Predicted BAF based on Log P: 

The following value was found for Log P: 

A value of 3.62 was selected-as a ' typical Log P., from which 

the following were obtained.: - 

3.62 MedChem Calc 

Predicted BCF (at 7.6% lipids) = 288.2 

Normalized BCF (at 1.0% lipids) = 37.9 

Food Chain Multiplier = 1.0 

2 
> No measured BCF or BAF was found. 

ACROLEIN [CAS#: 107-02-81 . 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

-0.01 MedChem Star , 0.101 MedChem Calc 

0.90 Isnard and Lambert 1989 

A value of 0.5 was selected as a 'typical Log P', from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) ='0.989 

Normalized BCF (at 1.0% lipids) = 0.130 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (0.130) (5.0) (1.0) 

= 0.65. 

> Predicted BAF based on Log P and measured BCF: 

BCF %L Norm BCF Reference 

(1.0% L) 

344 4 -8 71.7 Barrows et al. 1980 

Geometric mean normalized BCF = 71.7 

For -5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the geometric mean normalized .BCF is 

(71.7) (5.0) (1.0) = 358. 

.. 

Note: This BCF was based on uptake of radioactivity with no 

verification of the parent chemical. Thus this BCF and 

the resulting predicted HHBAF might be too high.. 

> No measured BAF was found. 

ACRYLONITRILE [CAS#: 107-13-1J. 

=- Predicted BAF based on Log P: 

" 

The following values were found for Log P: 

3 
0.25 MedChem Star 

0.231 MedChem Calc 

A value of 0.24 was selected as a 'typical Log P', from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 0.616 

Normalized BCF (at 1.0% lipids) = 0.081 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (0.081) (5.0) (1.0) 

= 0.40. 

> Predicted BAF based on Log P and measured BCF: 

BCF %L Nom BCF Reference 

(1.0% L) 

48 4.8 10.0 Barrows et al. 1980 

Geometric mean normalized BCF = 10.0 

For 5.4% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the geometric mean normalized 'BCF is 

(10.0) (5.0) (1.0) = 50. 

Note: This BCF was based on uptake of radioactivity with no 

verification of the.parent chemical.' Thus this BCF and 

the resulting predicted HHBAF might be too high. 

> No measured BAF was found. 

ALDRIN [CAS#:' 309-00-21 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

5 -09 MedChem Calc 

5.66 Isnard and Lambert 1989 

6.496 de Bruijn et al. 1989 

P 

A value of 6.0 was selected as a 'typical Log P', from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 21,878 

Normalized BCF (at 1.0% lipids) = 2,879 

Food Chain Multiplier = 67 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the "typic61 Log P" is (2,879) (5.0) (671 = 

964,465. -. - 

4 
> No BCF'with a measured value of percent lipids was found. 

> No measured BAF was . found. 

Note: Because of metabolism of aldrin to dieldrin, a 

predicted BAF for aldrin that does ,not take into 

account. the effect of metabolism on both the BCF and 

the FCM is expected to be higher than a measured BAF. . A BAF for 'aldrin plus toxic persistent metabolites' 

should be higher than a BAF for 'aldrin'. 

ALUMINUM (CAS#: 7429-90-51 

No usable data were found concerning bioconcentration or 

bioaccumulation of aluminum by freshwater fish. 

ANTHRACENE [CAS#: 120-12-71 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

4.45 MedChem Star 

4.49 MedChem Calc 

4.45 Veith et al. 1979a 

4.59 Hammers et al. 1982 

4.54 Miller et al. 1985 

4 -54 Isnard and Lambert 1989 

A value of 4.5 was selected as a 'typical Log P', from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 1,429' 

Normalized BCF (at 1.0% lipids) = 188 

Food Chain Multiplier = 1.2 

For"5.O$ lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (188) (5.0) (1.2) = 

1,128. 

> No measured BCF was found. 

> A geometric mean BAF of 192.5 was obtained with fish that 

averaged 4.23 % lipids (Burkhard, unpublished) , which results in 

a BAF of 228 at 5.0 % lipids. 

ANTIMONY [CAS#: 7440-36-01 

Barrows et al. (1980) did not detect any increase above controls 

5 
in whole-body measurements on bluegills exposed to antimony. 

Thus it seems reasonable to assume that bioconcentration and 

bioaccumulation of antimony by freshwater fish are negligible 

and the HHBAF will be set at 0.0. 

ARSENIC (CAS#: 7440-38-21 

Spehar et al. (1980) obtained BCFs of zero for various inorganic 

and organic forms of arsenic based on whole-body measurements on 

rainbow trout. Barrows et al. (1980) obtained a BCF of 4 using 

whole-body measurements on bluegills, and DeFoe (1982) found a 

BCF of 3 for whole body of fathead minnows. BCFs for muscle 

should not be higher than those for whole body; thus it seems 

reasonable to use a HHBAF of 1.0 for arsenic. 

ASBESTOS [CAS#: 1332-21-41 

No usable data were found concerning bioconcentration or 

bioaccumulation of asbestos by freshwater fish. 

1,2 -BENZANTHRACENE [CAS#: 56-55-31 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

5.664 MedChem Calc 

5.91 Miller et al. 1985 

5.91 Isnard and Lambert 1989 

A value of 5.8 was selected as a 'typical Log Pm, from which 

the fo'llowing were obtained: 

.'Predicted BCF (at 7.6% lipids) = 15,205 

Normalized BCF (at 1 .O% l'ipids) = 2,001 

Food Chain Multiplier = 33 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log Pm is (2,001) (5.0) (33) = 

330,165. 

=- No measured BCF was found. 

> Field data 

BAF of 40 

lipids. 

(Burkhard, unpublished) resulted in a geometric mean 

for two PAHs with ... four * rings for fish with 5.0 % 

- -. . 

6 
, 1 

BENZENE [CAS#: 71-43-21 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

2.13 MedChem Star 

2.142 MedChem Calc 

2.13 Veith et al. 1979a 

2.20 Harmners et al. 1982 

2.13 Miller et al. 1985 

2.12 Isnard and Lambert 1989 

2.186 de Bruijn et al. 1989 

A value of 2.14 was selected as a 'typical Log P', from which 

the following were ,obtained: 

Predicted BCF (at 7.6% lipids) = 19.5 

Normalized BCF (at 1.0% lipids) = 2.57 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log Po is (2.57) (5.0) (1.0) = 

13. 

> No acceptable measured BCF'was found. 

A BCF of over 5,000 was reported for fish with about 4% 

lipids by Brooke and Call (manuscript) . . The results were 

based on uptake of radioactivity. Results of this test were 

not used because metabolites were detected and probably 

contributed to the BCF. . 

> No measured BAF was found. 

BENZIDINE [CAS#: 92-87-51 

> Predict-e'd BAF based on Log P: 

The following values were found for Log P: 

1.34 MedChem Star 

1.576 MedChem Calc 

A value of 1.46 was selected as a 'typical Log P', from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 5.67 

Normalized BCF (at 1.0% lipids) = 0.75 . 

Food Chain Multiplier = 1.0 

For 5.0% lisids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (0.75) (5.0) (1.0) = 

3.8. " - -. 

> No acceptable measured BCF was found. 

7 
Barrows et al. (1975) conducted a bioconcentration test on 

benzidine and measured uptake of radioactivity. Results of 

this. test were not used because the tissue was air-dried 

before analysis. Also, metabolites might have caused the BCF 

to be too high. 

> No measured BAF was found.. 

3,4 -BENZOFLUORANTHENE [CAS#: 205-99-2 J 

> Predicted BAF based on Log P: 

The following value was found for Log P: 

A value of 6.12 was selected as a 'typical Log P', from which 

the following were obtained: 

6.12 MedChem Calc 

Predicted BCF (at 7.6% lipids) ='27,214 

Normalized BCF (at 1.0% lipids) = 3,581 

Food Chain Multiplier = 75 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (3,581) (5.0)(75) = 

1,342,075. 

Note: Field data (Burkhard, unpublished) resulted in 

predicted BAFs from 17 to 228 for four PAHs with three 

and four rings for fish with 5.0 % lipids. It seems 

unlikely that PAHs with five rings will have EAFs 

greater than 1000. 

> No measured BCF .or.BAF was found. 

11,12-BENZOFLUORAN"HENE [CAS#: 207-08-91 

7 Predicted BAF based on Log P: 

The following value was found for Log P: 

A value of 6.12 was selected as a 'typical Log P', from which 

the following were obtained: 

6.12 MedChern Calc 

Predicted BCF (at 7.6% lipids) = 27,214 

Normalized BCF (at 1.0% lipids) = 3,581 

Food Chain Multiplier = 75 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the "typic31 Log P' is (3,581) (5.0) (75) = 

1,382,875. -. - 

Note: Field data (Burkhard, unpublished) resulted in 

predicted BAFs from 17 to 228 for four PAHs with three 

8 
and four rings for fish with 5.0 % lipids. It seems 

unlikely that PAHs with five rings will have BAFs 

greater than 1000. 

> No measured BCF or BAF was found. 

1,12 - BENZOPERYLENE , [CAS#: 191-24-21 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

6.58 MedChern Calc 

7.10 .Miller et al. 1985 

7.10 Isnard and Lambert 1989 

A value of 6.84 was selected as .a 'typical Log P'', from which 

the following were obtained: . 

Predicted BCF (at 7.6% lipids) = 100,000 

Normalized BCF (at 1.0% lipids) = 13,158 

Food Chain Multiplier = 1.0 ' 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (13,158) (5.,0) (1.0) 

= 65,790. 

Note: Field data (Burkhard, unpublished) resulted in 

predicted BAFs from 17 to 228 for four PAHs with three 

and four rings for fish with 5.0 % lipids. It seems 

unlikely that PAHs with five rings will have BAFs 

greater than 1000. 

> No measured BCF or BAF was found. 

.- 

BEmO fa I PYRENE [CAS#: 50-32-81 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

5.97 MedChern Star 

6.12 MedChem Calc 

5 .9.8 Miller et al. 1985 

6.00 Isnard and Lambert 1989 

A value of 6.02 was selected as a ' typical Log P' , from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 22,688 

Normalized BCF (at 1.0.% lipids) = 12,985 

Food Chain Multiplier, = 67 - 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the "typical Log P' is (2,985) (5.0) (67) = 

999,975. 

9 
Note : Field data (Burlchard, unpublished) resulted in 

predicted BAFs from 17 to 228 for four PAHS with three 

and four rings for fish with 5.0 % lipids. It seems 

unlikely .that PAHs with five rings will have BAFs 

greater than 1000. 

> No measured BCF with a measured value of percent lipids was 

> No measured BAF was found. 

found . 

BERYLLIUM (CAS#: 7440-41-71 

Barrows et al. (1980) obtained a .BCF of 19 based on wholebody measurements on bluegills. A BCF based on edible-. 

portion might be lower, but the predicted H?iBAF will be set 

at 19 for lack of additional information. 

BIS(2-CHLOROETHOXY)ME"HANE [CAS#: 111-91-11 

> Predicted BAF based on Log P: 

The following value was found for Log P: 

A value of 0.75 was selected as a 'typical Log Pa, from which 

the following were obtained: 

0.75 MedChem Calc 

Predicted BCF (at '7.6% lipids) = 1.56 

Normalized BCF (at 1.0% lipids) = 0.205. 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (0.205) (5.0)(1.0) 

= 1.0. 

> No measured BCF or BAF was found. 

BI S ( 2 -CHLOROETHYL 1 ETHER [CAS#: 111-44-41 

> Predicted BAF based on Log P: 

The followina values were found for Log P: 

1.59 

0.996 

1.12 

A value of 1.14 was 

the following were 

MedChp Star 

MedChBn Calc 

Isnard and Lambert 1989 

selected as a "typical Log P', from which 

obtained: 

10 
Predicted BCF (at 7.6% lipids) = 3.17 

Normalized BCF (at 1.0% lipids) = 0.417 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (0.417)(5.0)(1.0) 

= 2.1. 

> Predicted BAF based on Log P and measured BCF: 

BCF %L Norm BCF Reference 

(1.0% L) 

11 4.8 2.29 Barrows et al. 1980 

Geometric mean normalized BCF = 2.29 

For 5.0% lipids at trophic level 4.the predicted HHBAF that 

is .calculated from the geometric mean normalized BCF is 

(2.29) (5.0) (1.0) = 11. 

Note: This BCF was based on uptake of radioactivity with no 

verification of the parent chemical. Thus this BCF and 

the resulting predicted HHBAF might be too high. 

> No measured BAF was found. 

BIS (2-CHLOROISOPROPYL) ETHER [CAS#: 108-60-11 

> Predicted BAF based on Log P: 

The following value was found for Log P: 

.A value of 1.61 was selected as a 'typical Log P', from which . the'-following were obtained: 

1.61 MedChem Calc . 

Predicted BCF (at 7.6% lipids) = ,7.45 

Normalized BCF (at 1.0% lipids) = 0.980 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (0.980) (5.0) (1.0) 

= 4.9. 

> No measured BCF or BAF was found. 

BROMOFORM [CAS#: 75-25-21 

> Predicted BAF based on Log P: 

11 
The. following value was found for Log P: 

A value of 2.37 was selected as a 'typical Log P', from which 

the following'were obtained: 

2.372 MedChan Calc 

Predicted BCF (at 7.6% lipids) = 29.7 . 

Normalized BCF (at 1 .O$ lipids) = 3.91 

Food Chain Mcltiplier E 1.0 

For 5.0% lipids at trophic level 4 the.predicted HHBAF that 

is calculated from the 'typical Log Pm is 43.91) (5.0) (1.0) = 

20. 

I 

> No measured BCF or BAF was found. 

4-BROMOPHENYL PHENYL ETHER [CAS#: 101-55-31 

. > Predicted BAF based on Log P: 

The following value was found for Log P: 

5.24 MedChem Calc . . A value of 5.24 was selected as a 'typical Log P', from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 5,490 

Normalized BCF (at 1.0% lipids) = 722.4 

Food Chain Multiplier = 4.3 

For 5 .O% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log PL is (722.4) (5.0) (4.3) 

= 15,532. 

> No measured' BCF or BAF .was found. 

> Predicted BAF based on Log P: 

The following values.were found for Log P: 

4.91 MedChem Star 

4.05 Isnard and Lambert 1989 

4.87 MedChem Calc 

A value of 4.7 was selected as a 'typical Log Pm, from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 2,056 

Normalized BCF (at 1.0% lipids) = 270.5 

Food Chain Multiplier = 1.4 

For 5.0% lipids at trophic .level 4 the predicted mBAF that 

is calculated from the 'typicZ1 Log P' is (270.5) (5.0) (1.4) E 1,894. 

12 
> Predicted BAF based on Log P 

BCF %L Norm BCF 

(1.0% L) 

6 63 4.8 138.1 

Geometric mean normalized 

and measured 

Reference 

~ ~~ 

BCF : 

Barrows et al. 1980 

BCF = 138.1 

Note: This BCF was based on uptake of radioactivity with no 

verification of the parent chemical. Thus this BCF and 

the resulting predicted KHBAF might be too high. 

> No measured BAF was found. 

CADMIUM [CAS#: 7440-43-9 J 

Based on dry-weight measurements on adu1.t brook trout, Benoit et 

al. (1976) obtained BCFs of 15 for muscle, 300 to 600 for whole 

bcdy, and 19,000 for kidney. Using a conversion factor of 0.2 

(Stephan et al. 19851, the wet-weight BCF for muscle is 

estimated to be 3. Sangalang and Freeman (1979) obtained a BCF 

of 17 for muscle of brook trout. Calamari et al. (19821 

obtained a BCF of 2.3 for muscle of rainbow trout. Data 

presented by Murphy et al. (19781 can be used to calculate BAFs 

of 0.3 and 1.6 based. on increases in the concentrations of 

cadmium in water and in muscle of bluegills and bass in a 

contaminated pond. The geometric mean of 0.3 and 1.6 is 0.69, I 

which will be used as the predicted HHBAF. .. 

CARBON TETRACHLORIDE [CAS#: 56-23-51 

> Predicted BAF based on Log P: 

me following values were found for Log P: 

2.83 MedChern Star 

2.875 MedChem Calc 

2.64 Isnard and Lambert 1989 

A value of 2.8 was selected as a 'typical Log P', from which 

the following were obtained: 

Predicted BCF (at 7.6's Pipias) = 64.9 

Normalized BCF (at 1.23% lipids) = 8.54 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

13 
> Predicted BAF based on Log P and measured BCF: 

BCF %L Norm BCF Reference 

(1.0% L) 

7,097 3.2 2,218 Goodman et al. 1978 

15,687 3.6 4,357 Parrish et .al. 1978 

37,800 7.6 4,974 Veith et al. 1979b . 

17,321 7.28 2,379 Oliver and Niimi 1985 

18,762 7.28 2,577 Oliver and Niimi 1985 

Geometric mean normalized BCF = 3,118 

The BCFs of 18,"762 and 17,321' from Oliver and Niimi (19'85) 

are for the alpha and ganaM isomers, respectively; both of . these values and the value for % lipids are the geometric 

means from two exposures. The BCFs might not be at steady . 

HHBAF that is calculated from the geometric mean normaliz

BCF is (3,118)(5.0)(33) = 514,470. 

" state. For 5.0% lipids at trophic ' level 4 the predicted 

> Measured Bioaccumulation Factors: 

(based on field data for fish at trophic level 4) 

BAF % L Norm BAF Species Reference 

(1.0% L) 

1,400,000 8 175,000 R. trout Oliver and Niimi 1985 

76,000 8 9,500 R. trout Oliver and Niimi 1985 

558, a24 11 50 , 802 Salmon Oliver and Niimi 1988 

Geometric' mean normalized BAF = 43,875. 

The BAF of 1,400,000 is for the alpha isomer, whereas the 

other two are for the gamma isomer. The range of the three 

normalized BAFs is a factor of 18, but there is no basis for 

choosing a subset of these' three values. For. 5 .O% lipids at 

trophic level 4 the predicted HHBAF is (43,875) (5.0) = 

219 , 375. 

CHLOROBENZENE [CAS#: 108-90-71 

> Predicted BAF based on, Log P: 

The following values were found for Log P: 

2.84 MedChem Star 

2.855 MedChkn Calc 

2.83 Hammers et al. 1982 

2.98 Miller et al. 1985 

2.80 Bobra et al. 1985 

15 
is calculated fromthe 'typical Log P' is (8.54)(5.0)(1.0) = 

43. 

> Predicted BAF based on Log P and measured BCF: 

BCF . % .L Nom BCF Reference 

(1.0% L) 

30 4 .a 6.25 Barrows et al. 1980 

Geometric mean normalized BCF = 6.25 

For 5.0% lipids at trophic level 4 the predicted H?IBAF that 

'is calculated from the' geometric mean normalized BCF is 

(6.25) (5.0) (1.0) = 31. 

Note: This BCF was based on uptake of radioactivity with no. 

verification of the parent chemical. Thus this BCF and 

the resulting predicted HHBAF might be too high. 

> No measured BAF was found. 

CHLORDANE [CAS#: see below] 

There are several isomers of chlordane and so the following were 

all considered to be of interest: 

CAS# : 57-74-9 Chlordane, mixture of cis and trans 

CAS#: 5103-71-9 alpha-chlordane; cis-chlordane 

CAS#: 5103-74-2 beta-chlordane; trans-chlordane 

CAS#: 5566-34-7 gannna-chlordane. CAS#,: 127.89-03-6 Chlordane, technical 

All of these are expected to have similar values for Log P, BCF, 

and BAF. 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

5.54 MedChem Calc 

6.0 Isnard and Lambert 1989 

A value of 5.77 was selected as a 'typical Log P', from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 14,398 

Normalized BCF (at 1.03 lipids) = 1,894 

Food Chain Multiplier = 33 

For 5 -0% lipids at trophic. lecel 4 the predicted HHBAF that 

is calculated from the "typical Log P'is (1,894) (5.0) (33) = 

312,510. 

14 
2.98 Isnard and Lambert 1989 

2.898 de Bruijn et al. 1989 

Values ranging from 2.18 to 2.98 were cited by Brooke et al. 

(1990). A value of 2.88 was selected as a 'typical Log P', 

from which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 75.0 

Food Chain Multiplier = 1.0 

' Normalized 3CF (at 1.0% lipids) = 9.87 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

'is calculated frornthe 'typical Log P' is (9.87) (5.0)(1.0) = 

49. 

> No acceptable measured BCF was'found. 

A BCF of over 400 was reported for fish with about 4% lipids 

by Brooke and Call (manuscript). The results were based on 

uptake of radioactivity. Results of this test were not used 

because metabolites were detected and. probably contributed to 

the. BCF. 

> No measured BAF was found. 

p-CHLORO-m-CRESOL . [CAS#: 59-50-71 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

3.10 MedChem Star 

3.134 MedChem Calc 

3.10 Veith et al. 1979a 

A value of 3.11 was selected as a 'typical Log P', from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 114.0 

Normalized BCF (at 1.0% lipids) = 15.0 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (15.0) (5.0) (1.0) = 

75. 

> No measured BCF or BAF was found. 

CHLORODIBROMOMETHANE [CAS#: 124-48-11 

> Predicted BAF based on Log P:. " - 

- 

The following value was found for Log P: 

A value of 2.23 was selected as a "typical Log Pm, from which 

2 -232 MedChem Calc 

16 
the following were obtained: 

Predicted BCF (at 7.6% lipids) = 23.0 

Normalized BCF (at 1.0% lipids) = 3.03 

Food Chain Multiplier = 1.0 

For 5 .O% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (3.03) (5.0) (1.0) = 

15. 

> No measured BCF or BAF was found. 

CHLOROETHANE [CAS#: 75-00-31 

> Predicted BAF based on Log P:, 

The following values were found for Log P: 

1.43 MedChem Star 

1.465 MedChem Calc 

A value of 1.44 was selected as a 'typical Log P', from which 

the following were obtained: 

Predicted BCF (at .7.6% lipids) = 5.47 

Normalized BCF (at 1.0% lipids) = 0.720 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the-predicted HHBAF that 

is calculated from the 'typical Log P' is (0.720) (5.0) (1.0) 

= 3.6. .. 

> No measured BCF or BAF was found. 

2-CHLOROETHYL VINYL ETHER [CAS#: 110-75-83 

> Predicted BAF based on Log P: . The following value was found for Log P: 

A value of 0.99 was selected as a 'typical Log Pa, from which 

the following were obtained: 

0.994 MedChem Calc .. 

Predicted BCF (at 7.6% lipids) = 2.41 

Normalized BCF (at 1.0% lipids) = 0.317 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HKBAF that 

is calculated from the "typical Log P' is (0.317)(5.0) (1.0) = 1.6. . .. 

> No measured BCF or BAF was foGd'. 

17 
CHLOROFORM [CAS#: 67-66-31 

> Predicted BAF based on Log P: 

me following values were found for Log P: 

1.97 MedChem Star 

1.952 MedChem Calc 

1-90 Isnard and Lambert 1989 

A value of 1.94 was selected as 'typical Log P', from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 13 -6 

Normalized BCF (at 1.0% lipids) = 1.79 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (l.??) (5.0) (1.01 E 

9.0 

> Predicted BAF based on Log P and measured BCF:' 

BCF %L Nom BCF Reference 

(1.0% L) 

6 4.8 . 1.25 Barrows etal. 1980 

Geometric mean normalized BCF = 1.25 

Note: This BCF was based on uptake of radioactivity with no 

verification of the parent chemical. Thus this BCF and 

the resulting predicted HHBAF might be too high. 

> No measured BAF was found. 

2 -CHLORONAPHTHALENE [CAS#: 91-58-71 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

4.03 MedChem Calc 

4.07 Isnard- and Lambert 1989 

A value of 4 -05 was selected as a 'typical Log P', from which. 

the following were obtained: 

Predicted BCF (at 7.63 lipids) = 630.2 

Normalized BCF (at 1.0% lipids) = 82.9 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (82.9) (5.0) (1.0) = 

18 
414. 

> No measured BCF or BAF was found. 

2-CHLOROPHENOL [CAS#: 95-57-81 

> Predicted BAF based on Log P: 

me following values were found for' Log P: 

2.15 MedChem Star 

2.205 MedChem Calc 

2.16 'Isnard and Lambert 1989 

2.12 Hammers et al. 1982 

A value of 2.16 was selected as a 'typical Log P', from'which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 20.25 

Normalized BCF (at 1.0% lipids) = 2.664 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 fhe.predicted HHBAF that 

is calculated from the 'typical Log P' is (2.664)(5.0)(1.0) 

= 13. 

> Predicted BAF based on Log P and measured BCF: 

BCF %L Norm BCF Reference 

(1.0% L) 

214 4.8 44.6 Barrows et al. 1980 

Geometric mean normalized BCF = 44.6 

Note: This BCF was based on uptake of radioactivity with no 

verification of the parent chemical. Thus this BCF and 

the resulting predicted HHBAF might be too high- 

> No measured BAF was found. 

4-CHLOROPHENYL PHENYL ETHER [Cas#: 7005-72-31 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

5.09 MedChem Calc 

19 
4.08 Isnard and Lambert 1989 

A value of 4.6 was selected as a 'typical Log P'8 from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 1,714 

Normalized BCF (at 1.03 lipids) = 225.5 

Food Chain Multiplier = 1.3 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated fyom the "typical Log P' i's (225.5) (5.0) (1.3) 

= 1,466. 

! 

Note: Metabolism is likely to reduce the BCF and FCM enough 

to cause the actual BAF to be less than 1000. 

>' No measured BAF was found. 

CHLORPYRIFOS [CAS#: 2921-88-2) 

> Predicted BAF .based on Log P: 

The following values were found for Log P: 

4.96 MedChem Star 

4.685 MedChem Calc 

4.99 Isnard and Lambert 1989 

5.267 de Bruijn et al. 1989 

A value of 4.97 was selected as a 'typical Log P', from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 3,360 

Normalized BCF (at 1 .O% lipids) = 442:l 

Food Chain Multiplier = 2.6 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the "typical Log PL is (442.1.) (5..0) (2.61 

= 5,747. 

> NO measured BCF with a measured value of percent lipids was 

found . 

> Eaton et al. (1985) found normalized BAFs of 209 for the fathead 

minnow and 120 for the bluegill. The geometric mean of 209 and 

120 is 158.4. For 5.0% lipids at trophic level 4 the predicted 

HHBAF is (158.4) (5.0) = 792. 

CHROMIUM [CAS#: 7440-47-31 

20 
BCFs of 0.13 and 2.8 were obtained for muscle of rainbow trout 

by Buhler et al. (1977) and Calamari et al. (1982) , respectively. The geometric mean of 0.13 and 2.8 is 0.60, which 

will be used as the predicted HHBAF. 

CHRYSENE [CAS# : 218-01-9 J 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

5.664 MedChem Calc 

5.79 , Miller et al. 1985 

5.79 Isnard and Lambert 1989 

A value of -3 was selected as a 'typical-Log P., from which 

.the following were obtained: 

Predicted BCF (at 7.6% lipids) = 13,388 

Normalized BCF (at 1.03 lipids). = 1,762 

Food Chain Multiplier = 23' - 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (1,762) C&P_l (23) = 

202,630. 

> No measured BCF was found. 

> Field data (Burkhard, unpublished) resulted in a geometric mean 

BAF of 40 for two PAHs with four rings for fish with 5.0 % 

lipids. 

COPPER [CAS#: 7440-50-81 

Benoit' (1975) reported a BCF of zero for copper in muscle of the 

bluegill. Thus the predicted HHBAF will be Set at 0.0. 

.- 

CYANIDE [CAS#: 57-12-51 

No usable data were found concerning bioconcentration or 

bioaccumulation of cyanide by freshwater fish. 

2,4-13 [CAS#: 94-75-71 .. 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

21 
2.81 MedChem Star 

2.682 MedChem Calc 

3.00 Isnard and Lambert 1989 

A value of 2.83 was selected as a ’typical Log P’, from which 

the following were obtained: 

Predicted BCF .(at 7.6% lipids) = 68.5 

Normalized BCF (at 1.0% lipids) = 9.01 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated fromthe ’typical Log PD is (9.01) (5.0) (1.0) = 

45. 

> No measured BCF or BAF was found. 

- DDD (CAS#: see below] 

There are several isomers of DDD; the common ones are: 

p,p‘-DDD; 4,4‘-DDD CASU: 72-54-8 

O*P “DDD; 2,4’-DDD ‘CAS#: 53-19-0 m8 P “DDD; 3,4’-DDD CAS#: 4329-12-8 

All of the isomers,. and all mixtures of them, are expected to 

have similar values for Log P8 BCF, and BAF. 

> Predicted BAF based on Log P: 

The followipg values were found for Log P: 

6.02 MedChem Star 

6.21 MedChem Calc 

6.02 Veith et al. 1979a 

6.02 Isnard and Lambert 1989 

6.227 de Bruijn et al. 1989 

Values ranging from 6.02 to 6.21 were cited by de Bruijn et 

al. (1989). A value of ‘6.1. was selected as a ’typical Log 

PL, from which the fol were obtained: ”J Predicted BCF (a 7.6% ipids) = 26,242 

Normalized BCF (.at lipids) = 3,453 

Food Chain Multiplier 

- 

= 75 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the ’typical Log P’ is (3,453) (5.0) (75) = 

1,294,875. 

> NO measured BCF with fish and with ameasured value of percent 

lipids was found. .- . - 

- 

3r Measured Bioaccumulation Factors: 

(based on field data for fish at trophic level 4) 

22 
BAF % L NO^ BAF Species Reference 

(1.0% L) 

892,473 11 81,134 Salmon Oliver and Niimi 1988 

Geometric mean normalized BAF = 81,134. 

For 5.0% lipids at trophic level 4 the predicted HHBAF is 

(81,134) (5.0) = 405,670. 

Note: This value is probably too high because the fish were 

also exposed to DDT. Uptake and metabolism of DDT 

probably contributed to the concentration of DDD in the 

fish. 

- DDE [CAS#: see below] 

There are several isomers of DDE; the coxrunon ones are: 

p,p"DDE; 4,4'-DDE CAS#: 72-55-9 

0,p"DDE; 2,4'-DDE CAS#: 3424-82-6 

All of the isomers, and all mixtures of them, are expected to 

have similar values for Log P, BCF, and BAF. 

> Predicted BAF based on Log P: \ 

The following values were found for Log P: 

6.51 MedChem Star 

6.936 MedChem Calc 

5.89 Burkhard et al. 1985 

5.69 Isnard and Lambert 1989 

6.956 . de Bruijn et al. 1989 

Values ranging from 5.69 to 6.94 were cited by'de Bmijn et 

al. (1989). A value of 6.4 was selected as a 'typical Log ' 

P',..from which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 45,290 

Normalized BCF (at 1.0% lipids) = 5,959 

Food Chain Multiplier = 98 

For 5.0% lipids at trophic level 4 the .predicted HHBAF that 

is calculated from the 'typical Log P' is (5,959) (5.0) (98) = 

2,919,910. 

> Predicted BAF based on Log P and measured BCF: 

BCF % L Nom BCF Reference 

(1.0% L) .- 

52 , 000 7.6 6,842 - Veikh et al. 1979b 

180,000* 9.12 19,737* Kosian et al. 1981 

11,773- 7.28 1,617** Oliver and Niimi 1985 

23 
t = extrapolated from 28 days to steady state. 

*+ = below steady state; not used in the calculation of the 

geometric mean normalized BCF. 

Geometric mean normalized BCF = 11,621 

For 

is CaLcu. 

(11, --- - 

> Measured Bioaccumulation Factors: 

(based on field data for fish at trophic level.4) 

BAF % L Norm BAF Species Reference 

(1.0% L) 

18,000,ooo 8 2,250,000 Trout Oliver and Niid 1985 

11,315,789 11 1,028,708 Salmon Oliver and Niimi 1988 

Geometric mean normalized BAF = 1,521,379 

- DDT [CASU: see below] 

. There are several isomers of DDT and several pertinent CAS 

numbers; the comn ones are: 

p,p"DDT; 4,4'-DDT CAS# : 50-29-3 

o,p."DDT; 2,4'-DDT CAS#: 789-02-6 

DDT CAS#: 33086-18-9 

All of the isomers, and all mixtures of them, are expected to 

have similar values for Log P, BCF, and BAF. The values uaed 

for Log P8 BCF, and BAF herein were based only on DDT and not on 

'DDT plus metabolites. because the GLI human health criterion is 

based on a study in which the test organism were fed 

commercial-grade DDT, which consisted of 81% p,p'-DDT and 17.9% 

O,P' -DDT. ." - - 

> Predicted BAF based on Log P: 

The following values were found 

6.36 MedChem 

24 

for Log P: 

Star 
6.913 MedChern Calc 

6 -19 Veith et al. 1979a 

6.38 Hammers et al. 1982 

5.44 Burkhard et al. 1985 

5.75 Isnard and Lambert 1989 

5.98 Isnard and -Lambert 198'9 

6.914 de Bruijn et al. 1989 

Values ranging from 3.98 to 7.48 were cited by Brooke et al. 

(19901, de Bruijn et al. (19891, and Eadsforth and Moser . (19831. A value of 6.2 was selected as a 'typical Log P., 

from which the following were obtahed: 

Predicted BCF (at 7.6% lipids) = 31,477 Normalized BCF (at 1.0% lipids) = 4,142 . Food Chain Multiplier = 84 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

'is calculated from the 'typical Log P' is (4,142)(5.0)(84) = 

1,739,640. 

> Predicted BAF based on Log P' and measured BCF: 

BCF %L Nom BCF Reference 

(1.0% L) 

29,400 7.6 3 , 868 Veith et al. 1979b 

37,000 7.6 4 , 868 Veith et al. 1979b 

Geometric mean nonnalized BCF = 4,339 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the geometric mean normalized BCF is 

(4,339) (5.0) (84) = 1,822,380. . 

> Measured Bioaccumulation Factors: 

(based on field data for fish at trophic level 4) .. -. 

BAF % L Norm BAF Species Reference 

(1.0% L) 

4,210,526 11 382,775 Salmon Oliver and Niimi 1988 

For 5.0% lipids at trophic level 4 the predicted HHBAF is 

(382,775) (5.0) = 1,913,875; for 7.9% lipids at trophic level 

4 the predicted WLBAF is (382,775) (7.9) = 31023,922. 

A normalized BAF of 382,775 for trophic level 4 corresponds 

to a Log P of 6.2525. The validity of this back calculation 

can be demonstrated as follows: - 

For a Log P of 6.2525, the BCF that is predicted for fish 

containing 7.6% lipids is (antilog[ (0.79) (6.2525)-0.4]} = 

34,631.79. The BAF that is predicted for fish containing 

25 
5.0% lipids at trophic level 4 is (34,631.79/7.6) (5.0) (84) 

= 1,913,862; the BAF that is predicted for'Yish containing 

7.9% lipids at trophic l'evel 4 is /'34,631.79/-7.6) (7.9) (84) 

= 3,023,902. Both of these BAFs..-..agree.""well with the 

values given above, as they should. 

Thus for a Log P of 6.2525, by using the FCM of 29 for 

trophic level 3 instead of the FCM of 84 for trophic level 4, 

the. WLBAF that is prediaed--for.,.-fish containing 7.9% lipids 

at trophic level 3 ,is -27.6) (7.9) (29). = 1,043,966. 

Note: Because of metabolism of DDT to DDD Ad DDE, a ' 

predicted BAF for' DDT that does not, take into account 

the effect of metaboli'sm on both the BCF and the FCM is 

likely. to be higher than a measured. BAF. A BAF for 

'DDT plus toxic ,persistent metabolites' should be 

higher than a BAF for "DDT.. 

- DEHP [CAS#: 117-81-7 J 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

8.66 MedChem Star 

8.707 . MedChem Calc 

4.20 Isnard and Lambert 1989 

7.453 de Bruijn et al. 1989 

Values ranging from 4.20 to 9.64 were cited by de Bruijn et 

al. (1989) and Brooke et al. (1990). A value of 8.0 was 

selected as a 'typical Log P., from which the following were 

obtained: 

Predicted BCF (at 7.6% lipids) = lOO,OOO 

Normalized BCF (at 1.0% lipids) = 13,158 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is cakulated from the 'typical Log P' is (13,158) (5.0) (1.0) 

'= 65,790. 

> Predicted BAF based on Log P and measured BCF: 

BCF %L Norm BCF Reference 

(1.0% L) 

114 4.8 23 -75 Macek et al. 1979; Barrows et al. 

1980 

Geometric mean normalized BCF = 23.75 

For 5 -0% lipids at trophic- iekl 4 the predicted HHBAF that 

is calculated from the "geometric mean normalized BCF" is 

(23.75) (5.0) (1.0) = 119. 

26 
Note: This BCF was based on uptake of radioactivity with no 

verification of the parent chemical. Thus this BCF and 

the resulting predicted HHBAF might be too high. 

> No measured BAF was found. 

DIAZINON [CAS#: 333-41-51 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

3.81 MedChem Star 

3.707 MedChem Calc 

3.31 Isnard and Lambert 1989 

A value of 3.6 was selected as a 'typical Log P', from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 278.0 

Normalized BCF (at 1.0% lipids) = 36.6 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'tmical Log P' is (36.6) (5.0) (1.0) = 

183. 

> 'Predicted BAF based on Log P and measured BCF: 

BCF %L Nom BCF Reference 

(1.0% L) 

182 3.6' 50.56 Goodman et al. 1979 

247.9 8.6 28.83 Tsuda et al. 1989 

Geometric mean normalized BCF =' 38.18 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calcul.ated from the geometric mean normalized BCF is 

(38.18) (5.0) (1.0) = 191. 

> No measured BAF' was found. 

1,2:5,6-DIBENZAENE [CAS#: 53-70-31 

> Predicted BAF based on Log P: -. - 

The following values were fodd for Log P: 

6.50 MedChem Star 

6.838 MedChem Calc 

6.88 Burkhard et al. 1985 

27 
7.19 Miller et al. 1985 

7.19 Isnard and Lambert 1989 

A value of 6.84 was selected as a 'typical Log P',. from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 100,000 

Normalized BCF (at 1.0% lipids) = 13,158 

Food Chair, Multiplier = 1.0 ' 

For 5.0% lipids at trophic level 4 the predicted HHBA?? that 

is calculated from the 'typical Log P' is (13,158) (5.0) (1.0) 

= 65,790. 

Note: Field data (Burkhard, unpublished) resulted in 

predicted BAFs, from 17 to 228 for four PAHs with three 

and four rings for fish with 5.0 % lipids. It seems 

unliliely that PAHs with five rings will have BAFs 

greater than 1000. 

> No measured BCF or BAF was found. 

DIBUTYL PHTHALATE (CAS#: 84-74-21 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

4.72 MedChem Star 

4.689 MedChem Calc 

5 .I5 Veith et al. 1979a 

A value of 4 -8 was selected as a 'typical Log P', from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 2,466 

Normalized BCF (at 1.0% lipids) = 324.5 

Food Chain Multiplier = 1.6 

For 5:0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (324.5) (5.0) (1.6) 

= 2,596. 

Note: Metabolism is likely to reduce the BCF and FCM enough 

to cause the actual BAF to be less than 1000, 

> No measured 

found . 

> No measured 

BCF with a measured value of percent lipids was 

BAF was found. 

- 

1,2iDICHLOROBENZENE [CAS#: 95'-50-1] 

> Predicted BAF based on Log P: 
The following values were found for Log P: 

3.38 MedChem Star 

3.568 MedChem Calc 

3.34 Haxruners etal. 1982 

3.38 Miller et al. 1985 

3.40 Bobra et al.1985 

3.39 Isnard and Lambert 1989 

3.433 de Bruijn et al. 1989 

Values ranging from 3.34 to 3.65 were cited by de Bruijn et 

al. (1989). A value of 3.4 was selected as a 'typical Log 

Pm, from which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 193.2 

Normalized BCF (at 1.0% lipids) = 25.4 

Food Chain Multiplier = 1.0 

For 5 .O% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log Pg is (25.4) (5.0) (i.0) = 

127. 

> Predicted BAF based on Log P and measured BCF: 

BCF %L Nom BCF Reference 

(1.0% L) 

89 4.8 18.5 Barrows et al. 1980 

389 8.45 46.0 Oliver and Niimi 1983 

Geometric mean normalized BCF = 29.2 

Note: The BCF reported by Barrows et al. (1980) was based on ._ uptake of radioactivity with no verification of the - parent chemical and therefore might be too high. 

> Data presented by Oliver and Nicol (1982) for lake trout in Lake 

Ontario and Lake Huron indicate that the normalized BAF for 1,2- 

dichlorobenzene is greater than 21; the predicted HHBAF for 5.0% 

lipids would be greater than 105. 

1,3-DICHLOROBENZENE [CAS#: 541-73-11 

> Predicted BAF based on Log P: .- - 

The following values were foukd for Log P: 

3.60 MedChem Star 

3.568 MedChem Calc 

3.46 Hammers et al. 1982 

29 
3.48 Miller et al. 1985 

3.40 Bobra et al. 1985 

3.48 Isnard and Lambert 1989 

3.525 de Bruijn et al. 1989 

Values ranging from 3.38 to 3.95 were cited by Eadsforth and 

Moser (1983) and de Bruijn et al. (1989). Avalue of 3.5 was 

selected as a 'typical Log PD, from which the following were 

obtained: 

Predicted BCF (at 7.6% lipids) = 231.7 . Normalized BCF (at 1.0% lipids) = 30.5 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log PD is (30.5)(5.0)(1.0) = 

152. 

> Predicted BAF based on Log P and measured BCF: 

BCF %L Nom BCF Reference 

(1.0% L) 

66 4.8 13.75 Barrows et al. 1980 

557 8.45 65.9 Oliver and Niimi 1983 

97 3.79 25.6 Carlson and Kosian 1987 

Geometric mean normalized BCF = 28.52 

Note: The BCF reported by Barrows et al. (1980) was based on 

uptake of radioactivity with no verification of the 

* parent chemical and therefore might be too high. 

> Data presented by Oliver and Nicol (1982) for lake trout .in Lake 

Ontario and Lake Huron indicate that the normalized BAF for 1,3- 

dichlorobenzene is greater than 57.9; the predicted HHBAF for 

5.0% lipids would be greater than'290. 

1,4-DICHLOROBENZENE [CAS#: 106-46-71 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

3.52 MedCh-em Star 

3.568 MedCh'em Calc 

3.41 Hammers et al. 1982 

3.38 Miller et al.1985 

3.40 Bobra et al. 1985 

30 
3.38 Isnard and Lambert 1989 

3 -444 de Bruijn et al. 1989 

Values ranging from 3.24 to 3.65 were cited by de Bruijn et 

al. (1989) and Brooke et al. (1990). A value of 3.46 was 

selected as a 'typical.Log P', from which the following were 

obtained: 

Predicted BCF (at 7.6% lipids) = 215.5 

Normalized BCF (at 1.0% lipids) = 28.4 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 .the predicted HHBAF that 

is calculated from the 'typical Log P' is (28.4) (5.0) (1.0) = 

142. 

> Predicted BAF based on Log P and measured BCF: 

BCF 

97 

60 

50 

516 

674 

110 

296 

%L 

5.4 

4.8 

2.3 

8.45 

7.28 

3.79 

8.5 

Norm BCF 

(1.0% L) 

18 .O 

12.5 

21.7 

61.1 

92.6 

29 .O 

34.8 

Reference 

Konemann and van Leeuwen 1979 

Barrows et al. 1980 

Galassi et al. 1982 

Oliver and Niimi 1983 

Oliver and Niimi 1985 

Carlson and Kosian 1987 

Smith et al. 1990 

Geometric mean normalized BCF = 31.1 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the geometric mean normalized BCF is 

(31.1) (5.0) (1.01 = 156. 

Note: The BCF reported by Barrows et al, (1980) was based on 

'uptake of radioactivity with no verification of the 

parent chemical and therefore might be too high. 

> Data presented by Oliver and Nicol (1982) for lake trout in Lake 

Ontario and Lake Huron indicate that the normalized BAF for 1,4- 

dichlorobenzene is 5.96; the predicted HHBAF for 5 .O% lipids is 

30. 

3,3'-DICHLOROBENZIDINE [CAS#: 91-94-11 

> Predicted BAF based on Log P: _' 

The following values were fouhd for Log P: 

3.51 MedChem Star 

3.637 MedChem Calc 

A value of 3.57 was selected as a 'typical Log P", from which 

31 
the following were obtained: 

Predicted BCF (at 7.6% lipids) = 263.2 

Normalized BCF (at 1.0% lipids) = 34.63 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (34.63) (5.0) (1.0 1 

= 173; 

> No measured BAF was found. 

DICHLOROBROMOMETHANE [CAS#: 75-27-41 

> Predicted BAF based on Log P: 

The following value was found for Log P:- 

A value of 2.09 was selected as a 'typical Log P', from which 

the following were obtairied: 

2.092 MedChem Calc 

Predicted BCF (at 7.6% lipi.ds) = 17.8 

Normalized BCF (at 1.0% lipids) = 2.35 

Food Chain MultipJier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (2.35)(5.0) (1.0) = 

12. 

> No measured BCF or BAF was found. 

1.1-DICHLOROETHANE (CAS#: 75-34-31 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

1.79 MedChem Star 

1.778 , MedChem Calc 

1.79 Isnard and Lambert 1989 

A value of 1.79 was selected as a 'typical Log P., from which 

the following were Obtained: 

Predicted BCF (at 7.6% lipids) = 10.3 

Normalized BCF (at 1.0% lipids) = 1.36 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic leyel 4 the predicted HHBAF that 

is calculated from the 'typical Log P" is (1.36) (5.0) (1.01 = 

6.8. 

32 
> No measured BCF or BAF was found. 

1,2-DICHLOROEm [CAS#: 107-06-21 

> Pr ,edicted BAF based on Log P: 

The following values were found for Log P: 

1.48 MedChem Star 

1.458 MedChem Calc 

1.45 Isnard and Lainbert 1989 

A value of 1.46 was selected as a 'typical Log P', 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 5..67 

Normalized BCF (at 1 .O% lipids) = 0.75 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted 

is calculated from 

" 

the 'typical Log P' 

3.6. 

> Predicted BAF based on Log P and measured 

from which 

HHBM that 

BCF : 

BCF %L Norm BCF Reference 

(1.0% L) 

2 4.8 0.417 Barrows et al. 1980 

Geometric mean normalized BCF = 0.417 

Note: This BCF was based on uptake of radioactivity with no 

verification of the parent chemical. Thus this BCF and 

"'the resulting predicted HHBAF might be too high. * 

> No measured BAF was found. 

1,l-DICHLOROETHYLENE [CAS#: 75-35-,4] 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

2.13 MedChq Star 

2.114 MedChW Calc 

A value of 2.12 was selected as a "typical Log Pa, from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 18.8 

33 
Normalized BCF (at 1.0% lipids) = 2.47 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated fromthe 'typical Log P' is (2.47)(5.0)(1.0) = 

12. 

> No measured BCF or BAF was found. 

1,2-trans-DICHLOROETHYLENE [CAS#: 156-60-51 

> Predicted BAF based on L0g.P: 

The following values were found for Log P: 

2.09 MedChem Star 

1.514 MedCheqn Calc 

A value of 1-80 was selected as a 'typical Log P', from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 10.5 

Normalized BCF (at 1.0% lipids) = 1.38 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (1.38)(5.0) (1.0) = 

6.9 

> No measured BCF or BAF was found. 

2,4-DICHLOROPHENOL [CAS#: 120-83-21 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

3.06 MedChem Star 

2.92 MedChem Star 

3.066 MedChm Calc 

3.08 Veith et al. 1979a 

2.92 Hammers et al. 1982 

3.08 Isnard and Lambert 1989 ' 

A value of 3.0 was selected as a 'typical Log P', from which 

the. following were obtained: 

Predicted BCF (at 7 -6% lipids) = 93.3 

Normalized BCF (at 1.0% lipids) = 12.3 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic-level 4 the predicted HHBAF that 

is calculated from the 'typita_l Log Pa is (12.3) (5.0) (1.0) = 

62. 

> No measured BCF or BAF was found. 

34 
. 1.2 -DICHLOROPROPANE [CAS#: 78-87-51 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

1.99 MedChem Calc 

2.00 Isnard and Lambert 1989. 

A value of 2.0 was selected as a 'typical Log P., from which . 

the. following were obtained: 

Predicted BCF (at 7.6% lipids) = 15.1 

Normalized BCF (at 1.0% lipids) = 1.99 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (1.99) (5.0)(1.0) = 

. 10. 

> No measured BCF or BAF was found. 

1,3 -DICHLOROPROPENE [CAS#: 542-75-61 

> Predicted BAF based on Log P: 

The following value was found for Log P: 

A value of 1.60 was selected as a 'typical Log P', from which 

the following were obtained: 

1.60 MedChem Calc 

Predicted BCF (at 7.6% lipids) = 7.31 

Normalized BCF (at 1.0% lipids) = 0.96 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated fromthe 'typical Log P' is (0.96) (5.0) (1.0) = 

4.8. 

.* 

=. No measured BCF or BAF' was found. 

DIELDRIN [CAS#: 60-57-11 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

4.32 MedChem Star 

2.916 MedChem Calc 

5 .ll Hmers et al. 1982 

5.16 Isnara and Lambert 1989 

5.40 de Bruijn et al. 1989 

Values ranging from 5.16 to 5.30 were cited by de Bruijn et 

al. (1989) . A Log P of 5.25 has been proposed for use in the 

35 
derivation of sediment quality criteria for' dieldrin (U.S. 

EPA lS91b). A value of 5.0 was selected as a 'typical Log 

P', from which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 3,548 

Normalized BCF (at 1.0% lipids) = 466.8 

Food Chain Multiplier = 2.6 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (466.8)(5.0) (2.6) 

= 6,068. 

> 'Predicted BAF based on Log P and measured BCF: 

BCF %L Norm BCF Reference 

(1.0% L) 

2,300 1.1 2,091 Parrish et al. 1974 

4,804 2.14 2,245 Shubat and Curtis 1986 

Geometric mean normalized BCF = 2,167 

For 5-08 lipids at trophic level 4 the predicted HHBAF that 

is calculated from the geometric mean normalized BCF is 

(2,167) (5.0) (2.6) = 28,171. 

> No measured BAF was found. 

DIETHYL PHTHALATE [CAS#: 84-66-21 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

2.47 MedChern Star 

2.57 MedChem Calc 

3.15 Veith et al. 1979a. 

3 .OO Isnard and Lambert 1989 

A value of 2.8 was selected as a 'typical Log P', from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 64.9 

Normalized BCF (at 1.0% lipids) = 8.54 

Food Chain Multiplier = 1.0 

For 5.0% lipids at tropnic level 4 the predicted HHBAF that 

is calculated from the "typical Log P' is (8.54) (5.0) (1.0) = 

43. 

> Predicted BAF based on Log P and'measured BCF: 

BCF %L Norm BCF Reference 

(1.0% L) 

36 
117 4 -8 24.38 Barrows et al. 1980 

Geometric mean normalized BCF = 24.38 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the geometric mean normalized BCF is 

Note: This BCF was based on uptake of radioactivity with no 

verification of the parent chemical. Thus this BCF and 

the resulting predicted HHBAF might be too high. 

> No measured BAF was found. 

2,4-DIMETHYLPHENOL [CAS#: 105-67-91 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

2.30 MedChem Star 

2.77 MedChem Calc 

2.36 Veith et al. 1979a 

2.42 Isnard and Lambert 1989 

A value of 2.48 was selected as a 'typical Log P', from which 

the following were obtained: 

Predicted BCF (at 7..6% lipids) = 36.2 

Food Chain Multiplier = 1.0 ~ Normalized BCF (at 1.0% lipids) = .4.76 

For 5:0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (4.76) (5.0) (1.0) = 

24. 

> Predict'ed BAF based on Log P and measured BCF: 

BCF %L Nom BCF Reference 

(1.08 L), 

150 4.8 31.25 Barrows et al. 1980 

Geometric mean normalized BCF = 31.25 

For 5.0% lipids at trophic level 4 the predicted MBAF that 

is calculated from the geometric mean normalized BCF is 

(31.25) (5.0) (1.0) = 156. 

Note: This BCF was based on. uptake .of radioactivity with no 

verification of the parent chemical. Thus this BCF and 

the resulting predicted HHBAF might be. too high. 

" 

37 
> No measured BAF was found. 

DIMETHYL P"I''E [CAS#: 131-11-31 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

1.56 MedChem Star 

1.52 MedChem Calc 

2.11 Veith et al. 1979a 

2.00 Isnard and Lambert 1989 

A value of 1.8 was selected as a 'typical Log P', from which 

the following were obtained: 

. Predicted BCF (at 7.6% lipids) = 10.52 

Normalized BCF (at 1.0% lipids) = 1.38 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHW that 

is calculated fromthe 'typical Log P' is (1.38) (5.0)(1.0) = 

6.9. 

> Predicted BAF based on Log P and measured BCF: 

BCF % L Norm BCF Reference 

(1.0% L) 

57 4.8 11.9 Barrows et al. 1980 

Geometric mean normalized BCF = 11.9 

Note: This BCF was based on uptake of radioactivity with no 

verification of the parent chemical. Thus this BCF and 

the resulting predicted HHBAF might be too high. 

> No measured BAF was found. 

4,6-DINITRO-o-CRESOL [CAS#: 534-52-11 

> Predicted BAF based on Log P: 

The following value was fowd.for Log P: 

A value of 2.56 was selected as a 'typical Log P', from which 

the following were obtained: 

2.56 MedChh Calc 

Predicted BCF (at 7.6% lipids) = 41.9 

38 
Normalized BCF '(at 1.0% lipids) = 5.51 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated fromthe 'typical Log P' is (5.51) (5.0) (1.0) = 

28. 

> No measured BCF or BAF was found, 

2,I-DINITROPHENOL [CAS#: 51-28-51 

> Predicted BAF based on Log P: 

.The following values were found for Log P: 

1.54 MedChem Star 

1.915 . MedChem' Calc 

1.51 Isnard 'and Lambert 1989' 

A value of 1.6 was selected as a 'typical Log P', fromwhich 

the following were.obtained: 

Predicted BCF (at 7.6% lipids)' =.7.31 

Normalized BCF (at 1.0% lipids) = 0.962 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (0,962) (5.0) (1.0) 

= 4.8. 

> No measured BCF or BAF was found. 

2.4-DINITROTOLUENE .[CAS#: 121-14-21. 

-> Predicted BAF. bas-ed on Log P: 

'. The-following values were found for Log P: 

1.98 MedChem Star 

1.997 MedChem Calc 

2.04 Deneer et al. 1987 

A value of 2.01 was selected as a 'typical Log P', from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 15.4 

Normalized BCF (at 1.0% lipids) = 2.03 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (2.03) (5.0) (1.0) = 10. " 

- 

> Predicted BAF based on Log P and measured BCF: 

BCF %L Nom BCF Reference 

39 
P 

(1.0% L) 

16.2 8 .O 2.025 Deneer et al. 1987 

Geometric mean normalized BCF = 2.025 

For 5 .O% lipids ar: trophic level 4 the predicted HHBAF that 

is calculated from 'the geometric mean normalized BCF is 

(2.025) (5.0) (1.0) = 10. 

> No measured BAF was found. 

2,6-DINITROTOLUENE [CAS#: 606-20-21 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

1.72 MedChem Calc 

2.02 Deneer et al. 1987 

A value of 1-87 was selected as a 'typical Log P', from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 11.9 

Normalized BCF (at 1.0% lipids) = 1.57 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (1.57) (5.0) (1.0) = 

7.8'. . . 

=. Predicted BAF based on Log P and measured BCF: 

BCF %' L Norm BCF Reference 

(1.0% L) 

21.9 802.74 Deneer et al. 1987 

Geometric mean normalized BCF = 2.74 

For 5.01 lipids at trophic level 4 the predicted HHBAF that 

is calculated from the geometric mean normalized BCF is 

(2.74) (5.0) (1.0) = 14. 

> No measured BAF was found. 

.- . 

-. - 

DIOCTYL PHTHALATE [CAS#: 117-84-01 

For lack of other information and because of the structural 

40 
similarity of the two chemicals, the predicted HHBAF derived for 

DEHP will also be used for dioctyl phthalate. 

1,2-DIPHENYLHYDRAZIN [CAS#: 122-66-71 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

2.94 MedChem Star 

2.97 MedChem Calc 

A value of 2.96 was selected as a ' typical Log P', from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 86.8 

Normalized BCF (at 1.0% lipids) = 11.4 

Food Chain Multiplier = 1.0 

For 5-0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical L0g.P' is (11.4)(5.0)(1.0) = 

57. . 

> No measured BCF or BAF was found. 

TNDOSULFAN [CAS#: 115-29-71 

The HHBAF derived below for the alpha and beta isomers of 

endosulfan will be also be used for endosulfan. 

alDha-ENDOSULFAN [CAS#: 959-98-81 

beta-ENDOSULFAN [CAS#.: 33213-65-91 

> Predicted BAF based on Log P: 

The following value was found .for Log P: 

A value of 3.83 was selected as a typical Log P., from which 

the following were obtained: 

3.83 MedChern Star 

Predicted BCF (at 7.6% lipids) = 422.4 

Normalized BCF (at 1.0% lipids) = 55.6 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the "typical Log P" is (55.6) (5.0) (1.0) t 

278. 

> No measured BCF with fish and- with a measured value of percent 

lipids was found. 

> No measured BAF was found. 

41 
a 

ENDOSULFAN SULFATE [CAS#: 1031-07-81 

> Predicted BAF based on Log P: 

The following value was found for Log P: 

A value of 3.07 was selected as a 'typical Log P',' from which 

the following were obtained: 

3.067 MedChem Calc 

Predicted BCF (at 7.6% lipids) = 106 

Normalized BCF (at 1.0% lipids1 = 13.9 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (13.9) (5.0)(1.0) = 

70. 

> No measured BCF or BAF was found. 

ENDRIN [CAS#: 72-20-81 

> Predicted BAF based.on Log P: 

The following values were found for Log P: 

4.32 MedChem Star 

2.916 MedChem Calc 

4.56 Veith et al. 1979a 

4.56 Isnard and Lambert 1989 

A Log P of 4.90 has been proposed for: use in the derivation 

of sediment quality criteria for endrin (U.S. EPA 1991~). A 

value of 4.44 was selected as a "typical Log P', from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) '= 1,281 

.Normalized BCF (at 1.0% lipids) = 168.6 

Food Chain Multiplier. = 1.1 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (168.6) (5.0) (1.1) 

= 927. 

> Predicted BAF based on Log P and measured BCF: 

BCF %L Norm BCF Reference 

(1.0% L) 

4,214 3.73 1,130 Jarvinen and Tyo 1978 

Geometric mean normalized BCF-= l;130 C. 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the geometric mean normalized BCF is 

42 
t 'I 

(1,130) (5.0) (1.1) = 6,215. 

> No measured BAF was found. 

ENDRIN ALDEHYDE [CAS#: 7421-93-41 

> Predicted BAF based on Log P: 

The following value was found for Log P: 

A value of 2.38 was selected as a 'typical Log P', from which 

the following were obtained: 

2.38 MedChem Calc 

Predicted BCF (at 7.6% lipids) = 30.2 

Normalized BCF (at 1.0% lipids) = 3.97 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated.from the 'typical Log P' is (3.'97) (5.0) (1.0) = 

20. 

> No measured BCF or BAF was found. 

ETHYLBENZENE ' [CAS#: 100-41-41 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

3.15 MedChem Star 

3.32 MedChem Calc 

3.26 Hammers et al. 1982 

3.13 Miller et al. 1985 

3 -13 Isnard and Lambert 1989 

A value of 3.22 was selected as a 'typical Log P', from which 

the following were obtained: 

Predicted BCF (at 7.6% lipids) = 139.3 

-Normalized BCF (at 1.0% lipids) = 18.33 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (18.33) (5.0) (3.0) . 

=. 92. 

> No measured BCF or BAF was found. 

43 
FLUOWWRENE [CAS#: 206-44-01 

> Predicted.W based on Log P: 

The following values were found for Log P: 

4 -95 MedChem Calc 

5 -22 Miller et al. 1985 

5.22 Isnard and Lambert 1989 

5.155 de Bruijn et al. 1989 

Values ranging from 5.148 to 5.29 were cited by de Bruijn 

et al. (1989) and Brooke et al. (1990). A Log P of 5.19 

was recommended for use in the derivation of. sediment 

quality criteria for fluoranthene (U.S. EPA 1991d). A 

value of. 5.11 was selected as a 'typical Log P', from which 

the following were obtained: 

Predicted BCF (at '7.6% lipids) = 4,334 

Food Chain Multiplier = 3:2 ' Normalized BCF (at 1.0% l'ipids) = 570.3 

For 5.0% lipids at trophic level 4 the predicted HHBAF that. 

is calculated from the 'typical Log E'. is (570.3) (5.0),(3.2) 

= 9,125. 

> No measured BCF was found. 

> A geometric mean BAF of 81 was obtained with fish that 

averaged 4.23 % lipids (Burkhard, unpublished), which results 

in a BAF of 96 at 5.0 % lipids. 

FLUORENE [CAS#: 86-73-71 

> Predicted BAF based on Log P: 

The following values were found for Log. P: . 4-18 MedChem Star 

4.225 MedChem Calc 

4.17 Hammers et al. 1982 

4.18 Miller et al. 1985 

4.38 .' Isnard and Lambert 1989 

A value of 4.24 was selected as a 'typical Log P.,. from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 890.4 

Normalized BCF (at 1.0% lipids) = 117.2 

Food Chain Multiplier = 1.1 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the "typical Log P' is (117.2) (5.0) (1.1) 

= 645. .- . .. 

> Predicted BAF based on Log P and measured BCF: 
BCF %L Norm BCF Reference 

(1.0% L) 

1,344 4.6 292.2 Carlson et al. 1979 

Geometric mean normalized BCF = 292.2 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the geometric mean normalized BCF is 

(292.2) (5.0) (1.1) = 1,607. 

> Field data (Burlchard, unpublished) resulted in a geometric 

mean BAF of 89 for fish with 5.0 % lipids for two PAHs with 

three rings .each. 

FLUORIDE [CAS#: 16984-48-81 

No usable data were found concerning bioconcentration or . bioaccumulation of fluoride.into muscle -of freshwater fish. 

GUTHION [CAS#: 86-50-01 

> Predicted BAF based on Log P: 

The following values were'found for Log P: 

2.75 MedChem Star 

2.62 MedChem Calc 

A value of 2 ..68 was selected as a 'typical Log P', from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 52.3 

Normalized BCF (at 1.0% lipids) = 6.86 

Food Chain Multiplier =. 1.0 

For 5.0% lipids at trophic level 4 the predicted' HHBAF that 

is calculated from the 'typical Log P' is (6.86) (5.0) (1.0) 

= 34. 

> No measured BCF or BAF was found. 

HEPTACHLOR [CAS#: 76-44-81 

The values for Log P, BCF, and BAF were based only on 

heptachlor and not 'on 'heptachior plus toxic persistent 

metabolites' because the human health criterion is based on a 

study in which the test organisms were fed heptachlor. 

45 
> predicted BAF based on Log P: 

The following values were .found for Log P: 

4.605 MedChem Calc 

5.44 Isnard 'and Lambert 1989 

A value of 5.02 was selected as a 'typical Log P', from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids) .= 3,680 

Food Chain Multiplier = 2.6 

. Normalized BCF (at 1.0% lipids) = 484.2 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P. is (484.2) (5.0) (2.6) 

= 6,295. 

> Predicted BAF based on Log P and measured BCF: 

BCF %L Norm BCF Reference 

(l.O%.L) . .- ._ 

2,999 i Schimel et al. 1976 

2,980 3.2 Goodman et al. 1978 

9,500 7.6 Veith et al. 1979b 

Geometric mean normalized BCF i_ , 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the geometric mean normalized BCF is 

(1,469) (5.0) (2.6) = 19,097. 

_, 

.. '1 6g;;) 

_-. 

> No measured BAF was. found. 

Note: Because of metabolism of heptachlor to heptachlor 

epoxide, a predicted BAF for heptachlor that does not 

take into account the effect of metabolism on both 

the BCF and the FCM is likely to be higher than a 

measured BAF. A BAF for. 'heptachlor plus toxic - 

persistent metabolites' should be higher than a BAF 

for 'heptachlor'. 

HEPTACHLOR EPOXIDE [CAS#: 1024-57-33 

- > Predicted BAF based on Log P: 

The following values were found for Log P: 

2.785 MedChem Calc 

5.40 Isnard and Lambert 1989 

A value of 4.09 was selected as a 'typical Log P', from 

which the following were obtained': 

Predicted BCF (at 7.6% lipids) = 677.8 

Normalized BCF (at 1.0% lipids) = 89.2 

46 
4 

Food Chain Multiplier = 1.1 

For 5.0% lipids at trophic level 4 the'predicted HHBAF that 

is calculated from the 'typical Log P' is (89.2) (5.0) (1.1) 

= 491. 

> Predicted BAF based on Log P and measured BCF: 

BCF %L ~orm BCF Reference 

(1.0% L) 

14,400 7.6 1,895 Veith et al. 1979b 

Geometric mean normalized BCF = 1,895 

> No measured BAF was found. 

HEXACHLOROBENZENE [CAS#: 118-74-11 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

5.31 MedChem Star 

6.42 MedChem Calc 

6.18 Veith et al. 1979a 

5.66 Hammers et al. 1982 

5.47 Miller et al. 1985 

6.86 Burkhard et al, 1985 

5 .SO Bobra etal. 1985 

5.66 Isnard and Lambert 1989 

Values ranging from 4.13 to 6.48 were cited by Eadsforth 

and Moser (1983) and Hammers et al. (1982) . A value of 5.7 

waa selected as a 'typical Log P', from which the 

following were obtained: 

Predicted BCF (at 7 -6% lipids) = 12,677 

Normalized BCF (at 1.0% lipids) = 1,668 

Food Chain Multiplier = 23 

.. 

5.73 de Bruijn et al. 1989 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from tAe 'typical Log P' is (1,668) (5.0) (23) 

= 191 , 820. .. 

> Predicted BAF based on Log P and measured BCF: 

BCF %L Nom BCF Reference 

47 
(1.0% L) 

18,500 7.6 . 2,434 Veith et al. 1979b 

15,660 5.4 2 , 900 Konemann and van Leeuwen 

1979,1980 

52,000 9.12 5,702 Kosian et al. 1981 

15,492 8.45 1,833 Oliver and Niimi 1983 

14,597 6.90 2,116 Nebeker et al. 1989 

22,000 3.79 5,805 Carlson and Kosian.1987 

27,000 5-00 5,400 Schrap and.Opperhuizen 1990 

Geometric mean normalized BCF = 3,366 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the geometric mean normalized BCF is . (3,366) (5.0) (23) = 387,090. 

> Measured Bioaccumulation Factors: 

(based on field data for fish at trophic level 4) 

BAF % L Norm BAF Species Reference 

(1.0% L) 

- c 45,700 L. trout Oliver and Nicol 1982 

545,000 7.9 68,987 R. trout Oliver and Niimi 1983 . 253,333 11 23 , 030 Salmon Oliver and Niimi 1988 

Geometric mean normalized QAF = 41,718 

For 5.0% lipids at trophic level 4 the predicted HHBAF is 

(41,718) (5.0) = 208,590. 

HEXACHLOROBUTADTENE ' [CAS# 87-68-31 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

4.78 MedChern Star 

4.78 Isnard and Lambert 1989 

4.30 MedChem Calc 

A value of 4.5 was selected as a 'typical Log P', from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 1,429 

Normalized BCF (at 1.0% lipids) = 188 

Food Chain Multiplier = 1.2 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typzcal Log P' is (188) (5.0) (1.2) = 

1,128. 

48 
> Predicted BAF based on'Log P and measured BCF: 

BCP- %L Norm BCF Reference 

(1.0% L) 

9,930 8.45 1,175 Oliver and Niimi 1983 

Geometric mean normalized BCF = 1,175 

> Measured Bioaccumulation Factors: 

(based on field data for fish at trophic level 4) 

BAF % L Nom BAF Species Reference 

(1.0% Ll 

4,000 7.9 506.3 Trout . 'Oliver and Niimi 1983 

Geometric mean normalized BAF = 506.3 

For 5.0% lipids at trophic level 4 the predic,ted HHBAF is 

(506.3) (5.0) = 2,532. 

HEXACHLOROCYCLOHEXANE [CAS#: 608-73-11 

alpha-HEXACHLOROCYCLOHEXANE [CAS#: 319-84-61 

beta-HEXACHLOROCYCLOHEXANE [CASU: 319-85-71 

delta-HEXACHLOROCYCLOHEXANE [CAS #: 319-86-81 

All data that. were found concerning BCFs ahd BAFs for . 

lindane. The same predicted HHBAF will be used for all forms 

of hexachlorocyclohexane; 

.hexachlorocyclohexane and its isomers are presented for 

HEXACHLOROCYCLOPENTADIE2h [CAS#: 77-47-41 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

5.04 MedChem Star 

.\ 4.645 MedChem Calc 

5.51 Isn.qrd and Lambert 1989 

A value of 5.2 was selected aB a "typical Log P', from 

which the following were obtained: 

Predicted BCF .(at 7.6% lipids) = 5,105 

Normalized BCF (at 1.0% lipids) = 671.7 

49 
> Predicted BAF based on Log P and measured BCF: 

BCF % L Norm BCF Reference . , 

(1 .O% L) 

29 7.6 3.82 Veith et al. 1979b 

Oliver and Niimi 11985) reported that 

hexachlorocyclopentadiene is not taken up by fish. 

Podowski et al. (1991) found that hexachlorocyclopentadiene 

is metabolized by fish. 

Geometric mean normalized BCF = 3.82 

For 5.0% lipids at trophic level 4 the predicted' HHBAF that 

is calculated from the geometric mean normalized BCF is 

(3.82) (5.0) (4.3') = 82. 

> No measured BAF was found. 

HEXACHLOROETHANE [CAS# '67-72-1 J 

> Predicted BAF based on Log P: 

The following values were found for Log P:. . 4.14 MedChem Star 

4.61 MedChem Calc . .. 3.93 Isnard and Lambert 1989 

A value of 4.2 was selected as a 'typical Log P', 'from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 827.9 

Normalized BCF (at 1.0% lipids) = 108.9 

Food Chain Multiplier = 1.1 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log Pm is (108.9)(5.0) (1.1) 

= 599. 

> Predicted BAF based on Log P and measured BCF: 

BCF % L Nom BCF . Re'ference 

.- . 

(1.0% L) 

139 4.8 28.96 Barrows et al. 1980 

50 
4 

782.3 8.45 92.58 Oliver and Niimi 1983 . 

Geometric mean normalized BCF = 51.78 

Note: The BCF reported by Barrows et al. (1980) was based 

on uptake of radioactivity with no verification of 

the parent chemical and might .be too high, 

> Measured Bioaccumulation Factors: 

(based on field data for fish at trophic level 4) 

BAF % L Norm BAF Species Reference 

(1.0% L) 

1, SO0 7.. 9 189.9 Trout Oliver and Niimi 1983 

Geometric mean normalized BAF = 189;s 

For 5.0% lipids at trophic level 4 the predicted HHBAF is 

(189.9) (5.0) = 950. 

1NDEN0~1,2,3-~dlP~W [CAS#: 193-39-51 

> Predicted BAF based on Log P: ' The following value was found for Log P: 

6.584 MedChem Calc 

A value of 6.58 was selected as a 'typical Log P', from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 62,835 

Normalized BCF (at 1.0% lipids) = 8,268 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHEAF that 

is calculated from the 'typical Log P' is (8,268)(5.0) (1.0) 

= 41,340. 

Note.: Field data (Burkhard, unpublished) resulted in 

predicted BAFs from 17 to 228 for four PAHS with 

three and four rings ior fish with 5.0 % lipids. It 

seems unlikely that PAHs with five rings will have 

BAFs greater than 1000. 

.. . 

> No measured BCF or BAF was' found. 
" 

IRON [CAS#: 7439-89-63 ' 

NO uoable data were found concerning bioconcentration or 

biouccnmrulation of iron by freshwater fish. 

ISOPHORONE [CAS#: 78-59-11 

-> Predicted BAF based on Log P: 

The following values were found for Log P: 

2.22 MedChem Calc 

1.67 Isnard and Lambert 1989 

A value of 1.8 was selected as a 'typical Log P', from 

which the following were'obtained: 

Predicted BCF (at 7.6% lipids) = 10.5' 

Normalized BCF (at 1.0% lipids) = 1.38 

Food Chain Multiplier = 1.0 . For 5.0% lipids at trophic level 4 the predicted HHBAF.that 

is calculated from the 'typical Log Pm is (1.38)(5.0)(1.0) 

= 6.9.- 

> Predicted BAF based.on Log P and measured BCF: 

BCF %L ~orm BCF Reference 

(1.0% L) 

7 4.8 1.46 Barrows et al. 1980 

Geometric mean normalized BCF = 1.46 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the geometric mean normalized BCF is 

(1.46)'(5.0) (1.0) = 7.3.. 

Note: This BCF was based on uptake of radioactivity with no 

verification of the parent chemical. Thus this BCF 

and the resulting predicted HHBAF might be too high. 

> No measured BAF was found. 

7 LEAD [CAS#: 7439-92-11 

Data presented by Atchison et al, (1977) can be used to 

calculate a BAF of 45.7 based on increased concentrations of 

lead in water and in the whole body of bluegills. Data 

presented by Murphy et al. (1978) can be used to estimate that 

BAFs for cadmium and zinc for muscle of the bluegill are about 

52 
4 

0 .I66 of those for the whole body of the bluegill. Thus the 

BA.F for lead in muscle of the bluegill can be estimated to be , 

(45.7)(0.366) .= 7.6, which will be used as the predicted 

HHBAP. 

LINDANE 

Lindane is the gamma isomer of hexachlorocyclohe&e, which is 

the pesticide 'BHC'. Thus the following were all considered 

to be of interest: 

Hexachlorocyclohexane (HCCH, HCH) CAS#: 608-73-1 

alpha-HCCH CAS#: 319-84-6 

beta-HCCH CAS#: 319-85-7 . ;gauma-HCCH (lindane) CAS#: 58-89-9 

delta-HCCH CAS#: 319-86-8 

All of these, and all of their mixtures, '.are expected to have 

similar values for Log P, BCF, and BAF. Because sufficient 

data are available for lindane, values for the other isomers 

are given for comparison purposes only. 

0 

> Predicted BAF based on Log P: 

The following values were found for Log P for lindane: 

3.61 MedChem Star 

3 -72 MedChem Star 

3.75 MedChem Calc 

3.85 Isnard and Lambert 1989 

3.69 de Bruijn et al. 1989 

MedChem contains Star Log P values of 3.80, 3.78, and 4.14 

for the alpha, beta, and delta isomers, respectively. 

Isnard and Lambert .(1989) and de Bruijn et ala (1989) cited 

Log P values of 3.77 to 3.96 .for the alpha'and beta 

isomers. A value of 3.7 was selected as a 'typical Log P., 

from which the 'following were obtained: 

Predicted BCF (at 7.6% lipids) = 333.4 

Normalized BCF {at 1.0% lipids) = 43.87 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted IWBAF that 

is calculated from the 'typical Log P* for lindane is 

(43.871 (5.0) (1.0) = 219. 

> Predicted BAF based on Log P and measured BCF for lindane: 

BCF %L ~orm BCF Reference 

(1.0% L) ." - 

180 7.6 23.68 -Veith et al. 1979b 

420 2.65 158 -5 Rogers et al.. 1983 

1549 7.28 212. a Oliver 'and Niimi 1985 

53 c 
Geometric mean normaaized BCF = 92.78 

[Oliver. and Niimi (1985) also reported a normalized BCF of 

269.3: for alpha-BHC; this value and the one given above 

frclnr Oliver and Niimi (1985) are means of two exposures. 

canton et al. (1975,1978) reported normalized BCFs of 45 

and 180.1 For 5.0% lipids at trophic level 4 the predicted 

HHBAF that is calculated from the geometric mean normalized 

BCF is (92.78) (5.0) (1.0) = 464. 

> Measured Bioaccumulation Factors for Lindane: 

(based on field data for fish at trophic level 4) 

BAF % L Nom BAF. Species Reference' 

(1.0% L) 

1,000 8 125. R. trout. Oliver and Niimi 1985. 

9,333 11 848.5 Sa llmon ' Oliver and Niimi. 1988 

Geometric mean normalized BAF = 325-7. 

[Oliver and Niimi (1985,1988) also reported normalized BCFs 

of 87 and 487 for alpha-BHC. Both values for alpha-BHC are 

lower than the corresponding values for lindane; in 

addition, both'values reported in 1985 are lower than the 

corresponding values reported in 1988.1 For 5.0% lipids at 

trophic level 4 the predicted HHBAF is (325.7) (5.0) = 

1,628. 

hmION [CAS#: 221-75-51 

> Predicted.BAF based on Log P: .. 

The. f.ollowing'values were found for Log P: 

2.36 MedChem Star 

1.756 MedChem Calc 

2.89 Isnard and Lambert 1989 

A value of 2.4 was selected as a Typical Log lP', from 

which the following were obtained: 

Predicted BCF . (at 7.6% lipids) = 31.3 

Normalized BCF (at 1.0% lipids) = 4.12 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (4.1'2) (5.0) (1:O) 

= 21. 

.C - 

s 

> Predicted BAF based on Log P and measured BCF: 

BCF %L Norm BCF Reference 

54 
(1.0% L) 

45'. 3.6 .' <6.9 Parrish et al. 1977 

3T&;.'-. 0. 6 4.40 Tsuda et al. 1989 

Geumetric mean noxnalized BCF = 4.40 

For 5 .O% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the geometric mean normalized BCF is 

> No measured BAF was found . 

MERCURY [CAS#: 7439-97-61 

1. The GLI human health criterion and the GLI wildlife criterion. 

for 'mercury' are both based on the toxicity of methylmercury 

[CAS#: 2296-92-61, whereas measurements .of mercury in water . usually determine 'total mercury'. Therefore the predicted 

HHBAF and WLBAF for "mercury' will be derived to relate the 

concentration of methylmercury in fish at trophic levels 3 

and 4 in the Great Lakes to the concentration of total 

mercury in .the water column. Although the best definition of 

the term 'total mercury' is probably 'the sum.of inorganic 

mercury and organic mercury., the concentration of 

methylmercury is often assumed to be a good approximation for 

the concentration of 'organic mercury'. In practice, all of 

these terms are operationally defined in terms of the 

analytical methods that are used to measure them in the.water 

column and in fish. 

2. Although methylmercury constituted less than 10% of the total 

mercury in the water column. in various portions of. a Canadian 

river system that had been contaminated with mercury. (Parks 

et al. 1989), Kudo et al. (1982) found that 33% of .the 

mercury in the Ottawa River was methylmercury. Gill and 

Bruland (1990) reported that at least 17%,of the mercury in 

Lake Erie was organo-mercury. Thus 25% would seem to be a 

reasonable estimate of the percent of the total mercury that 

is methylmercury in the water. column in the Great Lakes. 

3. A reasonable estimate of the BCF for inorganic mercury for 

freshwater fish is 2,998, which is the geometric mean.of 

1,800 and 4,994; these two BCFs are given on page 46 of the 

U.S. EPA criteria document farmercury (U.S. EPA 1985). Both 

of these BCFs are for whole bo&, but other data discussed .in 

the criteria document 'indicate that BCFs based on whole body 

and edible portion should be similar for mercuxy. This BCF 

of 2,998 relates the concentration of inorganic mercury in 
the water column to the concentration of total mercuxy in 

fish. (The B~P of 4,994 was used in the freshwater 

calculrtiona for inorganic mercury on page 47 in the criteria 

dodbecause the FDA action level is applied on a . 

speciu-by-species basis. 1 

4. 

5. 

6. 

7. 
in fish is methylmercujr, regardless of whether the form in 

the water was inorganic mercury or methylmercury.) 

.. 

8. 

I. I 

9. 

The Bc1 of 15,292 relates the concentration of total mercury 

in the water column (assuming that 25% of the total mercury 

in the water column is methylmercury) to the concentration of 

total mercury in fish. Based on the assumption that 85.3% of 

the total mercury in fish is methylmercury, a BCF of 

(15,292) (0.853) = 13,.044 can be used to relate the 

concentration of total mercury in the water column of the 

Great Lakes to the concentration of methylmercury in fish due 

to bioconcentration. (Using a BCF of 13,044 to relate total 

mercury in the water coluxnn to methylmetcury in fish is 

equivalent-t,o estimating that methylmercury constitutes 25% , 

of the-total.,: mercury in the water column and then using a B& 

5-10 relate methylmercury in the water column to' 

rcury in fish.) 

For predatory fish, accumulation of total mercury from food 

can add to the accumulation of total mercury from the water 

column. The following predator-prey factor increases in 

total mercury have been found for fish: ' 7.7 to 9.2 MacCrimnon et al. 1983 

8 and 15 Mathers and Johansen 1985 

up to 8.4 Wren et al. 1983 

up to 6 and 13 Skurdal et al. 1985 

2.9 Parks. 1988 

6.4 Cope et al. 1990 

It would seem reasonable to use a factor of 10 to estimate 

the importance of the food chain effect for some species. If 

the ratio of total mercury to methylmercury does not change 

substantially from prey to predator, the factor of 10 can be 

applied to methylmercury. 

10. Based on'paragraphs 8 and 9 above, a reasonable estimate of 

the Great Lakes is (13,044) (10) J.-34,440..? Thus the 

predicted HHW is 130,440 and is 'bded 1 to relate the 

concentration of methylmercury in trophic level 4 fish in the 

Great Lakes to the concentration of total mercury in the 

water column, 

. the BAF. .to be applied to total. xnerEi%ftin_.the water column in 

11. As stated in paragraph 3 above, it appears that BCFs and BAFs 

based on whole body and edible portion should be similar for 

mercury. Therefore, the predicted HHBAF that was derived for 

fish at trophic level 4 will also be used as the predicted 

WLBAF for fish at trophic level 4. On the assumption that 

the FCM for mercury increases.-geometrically with trophic 

level, the FCM will be assumed to be 2.154 for trophic level 

2, (2.154)(2.154) = 4.64 for trophic level 3, and 

(2.1541 (2.154) (2.154) = 10 for trophic level 4, Thus for 

mercury the predicted WLBAF for fish at trophic level 3 is 

57 
M€THOXYW& [CAS#: 72-43-51 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

5.08 MedChem Star 

5.325 MedChem Calc 

4.3 Isnard and Lambert 1989 

A value of 5.0 was selected as a 'typical Log P.8 from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 3,548 . Normalized.BCF (at 1.0% lipids) = 467 

Food Chain Multiplier = 2.6 

For 5.0% lipids at trophic level 4 the predicted that . is calculated from the 'typical -Log P' is (467) (S.Ol(2.6) = 

6,071. 

> Predicted BAF based on Log P 

BCF %L Nom BCF 

(1.0% L) 

151 3.6 42 

8,300 7.6 1,092 

-e two BCFs differ 

Geometric mean nonnalized 

and measured BCF: 

Reference 

Parrish et al. 1977 

Veith et al. 1979b 

substantially. 

BCF = 214 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

'is calculated from the geometric mean normalized BCF is 

(214)'(5.0) (2..6) = 2,782. 

> No measured BAF was found. 

METHYL BROMIDE [CAS#: 74-83-91 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

1.19 MedChem Star 

1.076 MedC-hgn Calc 

A value of 1.13 was selected as a"typica1 Log P'8 from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 3.11 

Normalized BCF (at 1.0% lipids) = 0.409 

58 
Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical 

= 2.0. 

> No measured XF or BAF was found. 

METHYL CHLORIDE [CAS#: 74-87-31 

> Predicted BAF based on Log P: . 

The following values were found 

0.91 MedChem 

0.936 MedChem 

A value of 0.92 was selected as 

Log P' is (0.409) (5.0) (1.0; 

for Log P: 

Star 

Calc 

a 'typical Log P., from 

which the following were obtained: . 

Predicted BCF (at 7.6% lipids] = 2.12 

Normalized BCF (at 1.0% lipids) = 0.28 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (0.28)(5.0)(1.0) 

= 1.4. 

> No measured BCF or BAF was found. 

METHYLENE CHLORIDE [CAS#: 75-09-2 J 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

1.25 MedChern Calc 

1.25 Isnard and Lambert 1989 . A value of 1.25 was selected as a 'typical Log P., from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids] = 3.87 

Normalized BCF (at 1.0% lipids) = 0.509 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (0.5091(5.01(1.0) 

= 2.5. 

> No measured BCF or BAF was found. 

59 
> predicted BAF based on Log P: 

The following values were found for Log P: 

5 -28 MedChem Star 

4.65 MedChem Calc 

6.89 Isnard and Lambert 1989 

A value of 5.3 was selected as a 'typical Log P', from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 6,124 

Normalized BCF (at 1.0% lipids) = 805.8 

Food Chain Multiplier = 5.8 

For 5.0% lipids at trophic level 4 the predicted HHBAF that . is calculated from t'he 'typical Log P. is (805.8) (5.0) (5.8). 

= 23;368. 

> predicted BAF based on Log P and measured BCF: . 

BCF % L Norm BCF Reference 

(1.0% L) 

18,100 7.6 2,382 Veith et al. 1979b 

20,417 6.5 3,141 . Gobas et al. 1989 

* = below steady state; not used in the calculation of the 

geometric mean normalized BCF. 

Geometric mean normalized BCF = 2,735 

740+ 7.6 97* . Oliver and Niirni 1985 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the geometric .mean normalized BCF is 

(2,735) (5.0) (5.8) = 79,315. 

> Measured Bioaccumulation Factors: 

(based on field data for fish at trophic level 4) 

BAF % L Norm BAF Species Reference 

(1.0% L) 

15,000,000 8 1,875,000 Trout Oliver and Niimi 1985 

5,806,452 11 527,859 Salmonids Oliver and Niimi 1988 

Geometric mean normalized BAF =' 994,855 

Note: Both of the measured- BA& are much larger than both 

of the predicted BAFs. 

60 
NAPHTHALENE [CAS#: 91-20-31 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

3.30 MedChem Star 

3.316 MedChem Calc 

3.37 Veith et al..l979a 

3 :45 Hammers et al. 1982 

3.35 Miller et al. 1985 

4.70 Isnard and Lambert 1989 

Values ranging from 3.01 to 3.45 are cited by Eadsforth and 

Moser (1983). A value of 3.36 was selected as a 'typical 

Log P', from which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 179.6 

Normalized BCF (at 1 .O% lipids) = 23.6 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF. that 

is calculated from the 'typical Log. P' is (23.6) (5.0) (1.0) 

= 118. 

> Predicted BAF based on Log P and measured BCF: 

BCF %L Nom BCF Reference 

(1.0% L) 

97.5 2.44 39.96 Rogers et al. 1983 

27.6 4.04 6.83 Brooke and Call, ms. 

Geometric mean-normalized BCF = 16.5 

Note: Brooke'and Call measured the BCF using radiolabeled . chemical and the parent chemical was verified. 

> No measured BAF was found. 

NICKEL [CAS#: 7440-02-01 

Calamari et al. (1982) obtained a BCF of 0.80 for muscle of 

rainbow trout. This value of 0.80 will be used as the 

predicted HHBAF. .. - 

NITROBENZENE [CAS#: 98-95-31 

61 
> Predicted BAF.based on Log P: 

The following values were found for Log P: 

1.85 MedChem Star 

1.885 MedChem Calc 

1.70 Hamers et al. 1982 

1.89 Deneer et al. 1987 

1.828 de Bruijn et al. 1989 

1.85 -1snard and Lambert 1989 

Values ranging from 1.70 to 1.88 were cited by de Bruijn et 

al. (1989) and Brooke et al. (1990). A value of 1.84 was 

selected as a 'typical Log Pm, from which the following 

were obtained: 

Predicted BCF (at 7.6% lipids) = 11.31 . 

Normalized BCF (at 1.0% lipids) = 1.45 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF .that 

= 7.2. 

. is calculated from the 'typical Log P9 is (1.45)(5.0)(1.0) 

Note: Brooke and Cail measured the BCF using radiolabeled 

chemical and the parent chemical was verified. 

> Predicted BAF based on Log P and measured BCF: 

BCF %L Norm BCF Reference 

(1.0% L)' 

2.34 8.0 0.293 ' Deneer et al. 1987 

2.49 4.04 0.616 Brooke and Call, ms. 

Geometric mean normalized BCF = 0.425 

For. 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the geometric mean normalized BCF is . (0.425) (5.0) (1.01 = 2.1. * 

> No measured BAF was found. 

2 -NITROPHENOL [CAS#: 88-75-51 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

1.79 MedChem Star 

1.854 MedChem Calc 

1.79 Isnaril and Lambert 1989 

A value of 1.82 was selected as a 'typical Log P', from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 10.91 

62 
Normalized BCF (at 1.0% lipids) = 1.44 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log Pm is (1.44) (5.0) (1.0) 

= 7.2. 

> No measured BCF or BAF was found. 

4 -NITROPHENOL [CASU: 100-02-71 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

1.91 MedChem Star 

1.854 MedChem Calc 

1.91 Veith et' al, S979a * 

2.91 Isnard and Lambert 1989 

A value of 1.9 was selected as a 'typical Log P', from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 12.62 

Normalized BCF (at 1.0% lipids) = 1.66 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (1.66) (5.0).(1.0) 

= 8.3. 

> Predicted BAF based on Log P and measured BCF: 

BCF %L Norm BCF Reference 

(1.0% Ll 

6.194' 4.04 1.533 Call et al. 1980; Brooke and 

Call, ms, 

Geometric mean normalized BCF = 1.533 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the geometric mean normalized BCF is 

(1.533) (5.0) (1.0) = 7.7. 

Note: The BCF was measured using radiolabeled chemical and 

the parent chemical was verified. The two predicted 

BAFs are similar. 

- 

> No measured BAF was found. ~. 

63 
N-NITROSODIMETHYLAMINE [CAS#: 62-75-91 

=. Predicted BAF based on Log P: 

The following values were found for Log P: 

A value of -0.67 was selected as a 'typical Log Pa, from 

which the following were obtained: 

-0.57 MedChem Star 

-0.768 MedChem Calc 

Predicted BCF (at 7.6% lipids) = 0 .ll8 

Normalized BCF (at. 1.0% lipids) = 0.0155 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBM that 

is calculated.from the 'typical Log P' is 

(0.0155) (5.0) (1.01 = 0.08. 

> No measured BCF or BAF was found. 

N-NITROSODIPHENYLAMINE [CAS#: 86-30-61 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

A value of 3.14 was selected as a 'typical Log P', from 

which the following were obtained: 

3.13 MedChem Star 

3.16 MedChem Calc - 

Predicted BCF (at 7.6% lipids) = 120.4 

Normalized BCF (at 1.0% lipids) = 15.8 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (15.8)(5.0) (1.0) 

= 79. 

> .Predicted BAF based on Log P and measured BCF: 

BCF % L Norm BCF Reference 

(1.0% L) 

217 4.8 45.2 Barrows et al. 1980 

Geometric mean normalized BCF = 45.2 

. 64 
> No measured BAF was found. 

I(- . 

N-NITROSODIPROPYLAMINE * [CASU: 621-64-71 

> Predicted BAF based on Log P: 

The following'values were found for Log P: 

1.36 MedChem Star 

1.348 MedChem Calc 

A value of 1.35 was selected as a 'typical Log P., from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 4.64 * 

Normalized BCF (at 1.0% lipids) =, 0.61 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that ' 

is calculated from the 'typical' Log P' is (0.61) (5.0) (1.0) 

= 3.0. 

> . No measured BCF or BAF was found. 

OCTACHLOROSTYRENE [CAS# 29082-74-43 

> ,Predicted BAF based on Log P:. 

The following value was found for Log P: 

A value of 7.94 was selected as a 'typical Log P', from 

which the following were obtained: 

7 -94 MedChem Calc' 

Predicted BCF (at 7 -6% lipids) = loo., 00.0 

Normalized BCF (at 1.0% lipids) = 13,158 

Food Chain Multiplier = 1.0 

For'5.0% lipids at trophic level 4 the predicted.HHBAF that 

is calculated from the 'typical Log P' is 

(13,158) (5.0) (1.0) = 65,790 

> Predicted BAF based.on Log P and measured BCF: 

BCF % L Nom BCF Reference 

(1.0% L) 

33,000 . 7.6 4,342 Veith et al. 1979b 

8,100 7.6 1,066 Oliver and Niimi 1985 

Geometric mean normalized BCF- = 2;151 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the geometric mean normalized BCF is 

65 
(2,151) (5.0) (1.0) = 10,755. 

> Measured Bioaccwrmlation Factors: 

(baaed on field data for fish at trophic level 4) 

BAF % L Nom BAF Species 'Reference 

(1.0% L) 

1,400,000 8 175,000 Trout Oliver and Niimi 1985 

9,361,702 11 851,064 Salmonids Oliver and Niimi 1988 

Geometric mean normalize'd BAF = 385,923 

For 5.0% lipids at trophic level 4 the predicted HHBAF is . (385,923) (5.0) = 1,929,615. 

PARATHION [CAS#: 56-38-21 

> Predicted BAF based on Log P: 

The. following values were found for Log P: 

3.83 MedChem Star 

3.469 MedChem Calc 

3.81 Isnard and Lambert 1989 

A value of 3..8 was selected as a 'typical Log P', from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 399.9 

Normalized BCF (at 1 .O% lipids) = 52.6 

Food Chain Multiplier .= 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (52.6) (5.0) (1.0) 

= 263. 

5 No measured BCF or BAF was found. 

PCBs (POLYCHLORINATED BIPHENYLS) 

The term 'PCBs' refers to a group of 209 chemicals, which are 

known as PCB congeners (for example, see Hawker and Connell 

1988). In addition, several commercial products are mixtures 

of PCBs; the most widely known such products are the ones 

called 'Aroclors'. Thus there are about 220 CAS numbers that 

pertain to PCBs. The GLI human health criterion is based on 

data for Aroclor 1260 (which is mostly hexa- and 

heptachlorobiphenyl), but the- criterion is considered 

representative for all PCB mixtures. 

66 
> Predicted BAF based on Log P: 

Because of uncertainty concerning the value of Log P for 

any one specific congener and because of the large number 

of individual chemicals that are covered under the term 

'PCBs', the following values for Log P are given as 

indicative of the range of values that are available: 

Class of PCBs 

Monochlorobiphenyls 

Lou P 

4.50 

4 -46-4 -69 

4.53 

4,26-4.95 

.Dichlorobiphenyls 

4.93-5.16 

5.14 

4.65-5.30 

4.96-4.98 

4.52-5.58 

Trichlorobiphenyls 

5'.47-5.51 

5.66 

5.02-5.89 

5.71-5.90 

5.47-5.61 

Tetrachlorobiphenyls 

Pentachlorobiphenyls 

Hexachlorobiphenyls 

Heptachlorobiphenyls 

5.72-5.73 

5.72-5.81 

5.21-6.48 

5..94-6.63 

5,.72-6.11 

5.92-6.30 

6.18 

6.04-6.95 

6.75 

5.92-6.30 

6.63-7.55 

5.48-6.68 

6'. 65 

6.22-7.42 

7.12-7.41 

6.63-6.93' 

67 

Reference 

Miller et al. 1985 

Hawker and Connell 1988 

de Bruijn et al. 1989 

Isnard and Lambert 1989 

Miller et al: 1985 

Burkhard and' Kuehl 1986 

Hawker and Connell 1988 

de Bruijn et al. 1989 

Isnard and Lambert 1989 

Miller et al. 1985 

Burkhard and Kuehl 1986 

Hawker and Connell 1988 

de Bruijn et al. 1989 

Isnard and Lambert 1989 

Miller et al. 1985 

Burkhard and Kuehl 1986 

Hawker and Connell 1988 

de Bruijn et al. 1989 

Isnard and Lambert 1989 

Miller et al. 1985 

Burkhard and Kuehl 1986 

Hawker and Connell 1988 

de Bruijn et al. 1989 

Isnard and Lambert 1989 

Miller et al. .1985 

Burkhard et al. 1985 

Burkhard and Kuehl 1986 

Hawker and Connell 1988 

de Bruijn et al. 1989 

Isnard and Lambert 1989 
L 

6.68 Miller et al. 1985 

6.69-7.71 Hawker and Connell 1988 

6.68 Isnard and Lambert 1989 

Octachlorobiphenyls 

7 .ll Miller 'et 'al. 1985 

7.20-8.00 Hawker and Connell 1988 

7 .ll Isnard and Lambert 1989 , 7.73 de Bruijn et al. 1989 

Nonachlorobiphenyls . . 

8.16 Miller et al. 1985 

7.71-8.09 Hawker and Connell 1988 

8.16 Isnard and Lambert 1989 

Decachlorobiphenyl 

8.26 

8.23 

8.18 

0.27 

8.26 

Aroclor 1016 

Aroclor 1242 

Aroclor 1248 

Aroclor 1254 

5.58 

5.58 

6 .ll 

6.72 

Miller et al. 1985 

Burkhard and Kuehl 1986 

Hawker and Connell 1988 

de Bruijn et al. 1989 

Isnard and Lambert 1989 

veith et al. 1979a 

Veith et al. 1979a 

Veith et al. 1979a 

Veith et al. 1979a 

Based on these ranges of values, a 'typical L0g.P. was 

selected for each class and then-a 'predicted HHBAF' was 

calculated for 5.0% lipids at trophic level 4: 

Class of mica1 

PCBs' Lou P 

MonochloroDichloroTrichloroTetrachloroPentachloroHexachloroHeptachloroOctachloroNonachloroDecachloro4.54 

4.99 

5.57 

5.75 

6.40 

6.82 

6.69 

7.45 

7.95 

8.24 

Pred BCF 

(7.6% L) 

1,537 

3,484 

10,007 

13,884 

45,290 

97,230 

76,754 

100,000 

100,000 

100,000 

Norm BCF 

(1.0% L) 

202 

458 

1,317 

1,827 

5,959 

12,793 

10,099 

13 , 158 

13 , 158 

13 , 158 

FCM .Pred HHBAF 

- (5.0% L) 

1.2 i, 2 12 

2.6 s, 954 

16 105,360 

23 210,105 

98 2,919,910 

1.0 63 , 965 

1.0 50,495 

1.0 65,790 

1.0 65, 790 

1.0 65,790 

> Predicted BAF based on Log P and-measured BCF: 

Class BCF % L Norm BCF Reference 

of PCBs - - (1.0% L) 

68 

.. . . 
4 

Dichloro14,100 

18,200 

10,000 

13,000 

6,100 

3, 800* 

Trichloro20,100 

42,000 

17,000* 

6,066 

14,125 

. Tetrachloro48,900 

42,000 

84,000 

18, OOOf 

17,000* 

6,806 

Pentachloro14,OOO* 

Hexachloro210,000 

4,800* 

Octachloro22 , 400. 

Decachloro10,500 

8,374 

Aroclor 1016 

24,299 

42,500 

Aroclor 1248 

70,500 

Aroclor 1254 

37,000 

6,601 

100,000 

Aroclor 1260 

194,000 

3 .O 

3.5 

7.6 

7.6 

11.6 

7.6 

3.0 

3.0 

7.6 

6.5 

7.5 

3.0 

3.0 

3.5 

7.6 

7.6 

6.5 

7.6 

3.5 

7.6 

3.5 

3.5 

6.5 

3.6 

7.6 

7.6 

1.1 

3 -16 

7.6 

7.6 

4,700 

5,200 

1,316 

1,711 

8 03 

328* 

6,700 

14,000 

2,237* 

933 

1, a83 

16,300 

14,000. 

24,000 

2; 368* 

2 , 237* 

1 , 047 

1 , 842* 

60 , 000 

632* 

6,400 

3,000 

1,288 

6,750 

5,592 

9,276 

33 , 636 

2,089 

13 , 159 

- 

Brugg-n et al, 1981 

Brugg-n et al. 1984 

Oliver and Niimi 1984 

Oliver and Niimi 1985 

Oliver and Niimi 1985 

Oliver and Niimi 1985 

Bruggeman et ale 1981 

Bruggeman et al. ' 1981 

Oliver and Niimi 1985 

Gobas et al. .1989 

Gobas and Schrap 1990 

Bruggeman et al. 1981 

Bruggeman eteal. 1981 

Bruggeman et al, 1984 

Oliver and Niimi 1985 

Oliver and .Niimi 1985 

Gobas et al. 1989 

Oliver and Niimi 1985 

Bruggeman et al. 1984 

Oliver and Niimi 1985 

Bruggeman et al, 1984 

Bruggeman et al, 1984 

Gobas et al; 1989 ' 

Hansen et al. 1975 

Veith et al. 1979b 

veith et al, 1979b 

Hansen et al. 1971 

Snarski and Puglisi 1976 

Veith et al. 1979b 

25 , 526 Veith et al. 1979b 

69 
.+ = below steady state. 

For several of these the range of the normalized BCF is

greater than a factor of 10. 

> Measured Bioaccumulation Factors: 

(based on field data for fish at trophic level 4) 

Class BAF % L Norm BAF Species 

Trichloro- 

--- (1.0% L) 

TetrachlorolO,OOO, 000 8 1.~250,OOO Trout . 8,125,000 11 738,636 Salmon 

Heptachloro11,296,296 1,026,936 Salmon 

Octachloro13,000,000 11 1,181,818 Salmon 

3,909,091 11 355,372 Salmon 

Total PCBs 

Reference 

Oliver and Niimi 1985 

Oliver .and Niimi 1988 

Oliver and Niimi i985" 

Oliver and Niimi 1985 

Oliver and Niimi 1988 

Oliver and Niimi 1985 

Oliver and Niimi 1988 

Oliver and Niimi 1985 

Oliver and Niimi 1988 

Oliver and Niimi 1988 

Oliver and Niimi 1.988 

Oliver and Niimi 1988 

The. normalized BAF of 355,372 that was determined for 

The predicted HHBAF of 1,776,860 for 5.0% lipids at trophic 

level 4 seem reasonable because it is similar to the 

predicted HHBAF that was calculated above using the 

"typical Log P' for pentachlorobiphenyl, which is a PCB 

with an intermediate amount of chlorination., 
A normalized BAF of 355,372 for trophic level 4 corresponds 

to a Log P of 6.21167. The validity of this back 

calculation can be demonstrated as follows: 

For a Log P of 6.21167, the BCF that is predicted for 

fish containing 7.6% lipids is (antilog[(0.79) (6.21167)- 

0.401) = 32,152.84. The BAF that is predicted for fish 

containing 5.0% lipids at trophic level 4 is 

(32,152.84/7.6)(5.0) (84) = 1,776,867; the BAF that is 

predicted for fish containing 7.9% lipids at trophic 

level 4 is (32,152.84/7.6) (7.9) (84) = 2,807,451. Both 

of these BAFs agree well with the values given above, as 

they should. 

Thus for a Log P of 6.21167; by using the 'FCM of .29 for 

trophic level 3 instead of the FCM of 84 for trophic level 

4, the WLBAF that is predicted for fish containing 7.9% 

.lipids at trophic level 3 is (32,152.84/7.6) (7.9)(29) = 

969,239. 

PENTACHLOROBENZENE [CAS# 608-93-51 

> Predicted BAF based on Log P: 

The following values we're found for Log P: 

5.17 MedChem Star 

5.707 MedChem Calc 

5.06 Hammers et al. 1982 

5.03 Miller et al., 1985 

5.00 Bobra et al.1985 

5.19 lsnard and Lambert 1989 

5.183 de Bruijn et al. 1989 

Values ranging from 4.88 to 5.92 were cited by de Bruijn et 

al. (1989). A value of 5.1 was selected as a 'typical Log 

p', from which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 4,256 

Normalized BCF (at' 1.0% lipids) = * 560 

Food Chain Multiplier = 3.2 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (5601 (5.0) (3.2) = 

8,960. 

> Predicted BAF based on Log P and measured BCF: 

BCF % L . Norm BCF 

(1.0% L) 

14,040 5.4 2,600 

3,400 4.8 708 

16,125 8.45 1,908 

7,100 1.8 3,944 

5,100 1.5 3,400 

Reference 

Konemann and van Leeuwen 

1979,1980 

Barrows et al. 1980 

Oliver and Niimi 1983 

Banerjee et al. 1984 

Banerjee et al. 1984 

71 
7,300 2.8 2 , 607 Banerjee et al. 1984 

4,700 5 940 Van Hoogen and Opperhuizen 

910 3.5 2 60 Bruggeman et al. 1984 

8,400 3.79 2,216 Carlson and Kosian 1987 

23,000 5 4,600 

1988 ~ --- 

Scrap and Opperhuizen i990 

Geometric mean normalized BCF = 1,772 

> Measured Bioaccumulation Factors: 

(based on field data for fish at trophic level 4) 

BAF % L Norm BAF . Species Reference 

(1.0% L). 

- - .2,538 L. trout Oliver and Nicol 1982 

17,000 7.9 2,152 Trout Oliver and Niimi 1983 

69,444 11 6,313 Salmonids Oliver and Niimi 1988 

Geometric mean normalized BAF = 3,255 

For 5.0% lipids at trophic level 4 the predicted HHBAF is 

(3,255) (5.0) = 16,275. 

PENTACHLOROPHENOL [CAS # : '8 7 - 8 6 "5 ] 

> Predicted BAF based on Log P: 

The following values were found for.Log P: 

5.12 MedChem Star 

5.24 MedChem Star 

5.055 MedChem Calc 

5.01 Veith et al. i979a 

5.01 Isnard and Lambert 1989 

A value of 5.1 was selected as a 'typical Log P', from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 4,256 

Normalized BCF (at 1.0% 3ipids) = 560 

Food Chain Multiplier. = 3.2 

For 5.0% lipids at trophic level 4 the predicted HHBAF 

is calculated from the "typical Log P" is (560) (5.0) (3 

that 

2) = 

72 

-___ 
> Predicted BAF based on Log P and measured BCF: 

BCF %L Norm BCF Reference 

(1.0% L) 

27* 3.6 7.5 Parrish et al. 1978 

770 7.6 101.3 Veith et al. 1979b 

216 13.25 16.30 Smith et al. 1991 

= In salt water. 

Geometric mean normalized BCF in fresh water = 40.63 

For S.O%.lipids at trophic level 4 the predicted HKBAF that 

is calculated from the geometric mean normalized BCF is 

(40.631 (5.0) (3.2) = 650. 

> No useful measured BAF was found. 

PHENANTHRENE [CAS#: 8s-01-81 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

4.46 MedChem Star 

4.49 MedChem Calc 

4.42 Hammers et al. 1982 

4.57 Miller et al. 1985 

4.562 de Bruijn et al. 1989 

4.46 'Isnard and Lambert 1989 

Values ranging from 4.10 to 4.67 were cited by Eadsforth 

and Moser (1983), de Bruijn et al. (1989), and Brooke et 

al. (1990). A Log P of 4.36 was recommended for use in the 

derivation of sediment quality criteria for phenanthrene 

(U.S. EPA 1991e). A value of 4.5 was selected as a 

'typical Log P', from which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 1,429 

Normalized BCF lat 1.0% lipids) = ,188 .O 

Food Chain Multiplier = 1.2 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the "typical Log P' is (188.0) (5.0) (1.2) 

= 1,128. 

" 

c 

> Predicted BAF based on Log P and measured BCF: 

BCF %L Norm BCF Reference 

73 
(1.0% L.) 

2 , 850 4.27 667.4 ' Carlson et al. 1979 

Geometric mean normalized BCF = 667.4 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the geometric mean normalized BCF is 

(667.4) (5.0) (1.2) = 4,004. 

> A geometric mean BAF of 30 was obtained with fish that 

averaged 4.23 % lipids (Burkhard, unpublished), which results 

in a BAF of 35 at 5.0 % lipids. .. 

PHENOL [CAS#: 108-95-21 

> Predicted BAF based on Log P: 

The following values were found for.Log P: 

1.46 MedChem Star 

1.475 MedChem Calc 

1.46 Veith et al. 1979a 

1 .ll Hammers et al. 1982 

1.46 Isnard and Lambert 1.989 

Values ranging from 0.62 to 2.20 were cited by Eadsforth 

and Moser (1983). A value of 1.4 was selected as a 

"typical Log P', from which the fallowing were obtained: 

Predicted BCF (a,t 7.6% lipids) = 5.08 

Normalized BCF (at 1.0% lipids) = 0.688 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the "typical Log Pm is (0.688)(5.0)(1.0) 

= 3.4.. ,. 

> Predicted BAF based on Log P and measured BCF: 

BCF %L Norm BCF Reference 

(1.0% L) 

1,382 4.0 345.5 Call et al. 1980; Brooke and 

Call, ms. 

Geometric mean normalized BCF = 345.5 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the geomettic mean normalized BCF is 

(345.5) (5.0) (1.0) = 1,728.- 

Note: The BCF was measured using radiolabeled chemical and 

74 

.- 

_" 
the parent chemical was verified. 

BAFs are very different. 

> No measured BAF was found. 

PHOTOMIREX -[CAS# 39801-14-41 

> Predicted BAF based on Log P: 

The following values were found for Log 

4.54 MedChein Calc 

The two predicted 

P: 

A value of 4.54 was selected as a 'typical Log PD, from 

.which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 1,537 

Nornralized BCF (at 1.0% lipids) .= 202.2 

Food Chain Multiplier = 1.2 

For 5.0% lipids at trophic level 4 the,predicted HHBAF that 

is calculated from the "typical Log P. is (202.2) (5.01 (1.21 

= 1,213. - 

> No measured BCF was found. 

> Measured Bioaccwnulation Factors: 

(based on field data for fish at trophic level 4) 

BAF % L Norm BAF Species Reference 

(1.0% L) 

5,117,647 11 465,241 Salmonids Oliver and Niimi 1988 

Geometric mean normalized BAF = 465,241 

For 5.0% lipids at trophic level 4 the predicted KHBAF is 

(465,241) (5.0) = 2,326,205. 

Note: The measured BAF is much larger than the predicted 

BAF. 

PYREWE [CAS#: 129-00-01 

> Predicted BAF based on Log P: 

The following values were fowd for Log P: 

4.88 MedChem Star 

4.95 MedChem Calc 

4.93 Hammers et al. 1982 

5.52 Burkhard et al. .1985 

7s 
5.18 Miller et al.1985 

4.88 Isnard and Lambert 1989 

A value of 5.1 was selected as a 'typical Log P', from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 4,256 

Normalized BCF (at 1-08 lipids) = 560 

Food Chain Multiplier = 3.2 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (560) (5.0) (3.2) = 

8,960. 

> Predicted BAF based on Log P and measured BCF: . 

. BCF %L Norm BCF Reference 

(1.0% L) 

2,445 4.4 555.7 Carlson et al. 1979 

Geometric mean normalized BCF = 555.7 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the geometric mean normalized BCF is 

(555.7) (5.0) (3.2) = 8,891. 

> A geometric mean BAF of 14.3 was obtained with fish. that 

averaged 4.23. % 9ipids (Burkhard, unpublished), which results 

in a BAF of 17 at 5.0 % lipids. 

SELENIUM [CAS#: 7782-49-21 

Adams (1976) obtained BCFs of 2 and 12 for muscle of the 

rainbow.trout and fathead minnow, respectively. Barrows et 

al. (1980) reported a whole-body BCF of 20 for the bluegill. 

The geometric mean of 2 and 12 is 4.9, which will be used as 

the predicted HHBAF for selenium. 

SILVER [CAS#: 7440-22-43 

Barrows et al. (1980) found no uptake of silver by the 

bluegill. The value of 0.0 will be used as the predicted 

HHBAF . 

2,3,7,8-TCDD [CAS#: 1746-01-61 
! 

> Predicted BAF based on Lbg P: 

The following values were found for Log P: 

7.02 MedChem Star 

7.695 MedChem Calc 

7.02 Burkhard and Kuehl 1986 

A value of 7.36 was 'selected as a 'typical Log P", from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 100,000 

No'rmalized BCF (at 1.0% lipids) = 13,158 . Food Chain Multiplier = 1.0 

For 5.0% lipids at' trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P. is 

(13,158) (5.0) (1.0) = 65,790. 

> Predicted BAF based on Log P and measured BCF:. A 

Cook et al. (1991) recommended a BCF of 51,OO.O for fish 

that. contain 7% lipids. Cook (1992) recommended use of. a 

BAF of 60,000 for fish that contain 5.0% lipids and are at 

trophic levels 3 and 4 in the Great Lakes. This is 

equivalent to recommending use of a FCM of 1.37255 for both 

trophic level 3 and trophic level 4. This can be 

demonstrated because for 'fish that are at trophic level 4 

and contain 6% lipids, the predicted BAF is 

(51,000/7)(6)(1.37255) = 60,000. Thus for fish that are at 

trophic level 4 and contain 5.08 lipids, the predicted 

HHBAF is (51,000/7) (5.0) (1.37255) = 50,000; similarly .for 

fish that contain 7.9% mBAFs are 

(51,000/7) (7.9) both trophic level 3 

and trophic 

1,2,3,4 -TETRACHLOROBENZENE [CAS#: 634-66-21 ' 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

4.64 MedChem Star 

4.99 MedChem Calc 

4.41 Hammers et al. 1982 

4.55 Miller et al. 1985 

4 .SO Bobra et al. 1985 

4.65 Isnard and Lambert 1989 

4.635 de Bruijn et al. 1989 

Values ranging from 4.41 to 5.16 were cited by Hammers et 

al. (1982) and de Bruijn et al. (1989). A value of 4.64 

was selected as a "typical Log P", from which the following 

were obtained: .. 

Predicted BCF (at 7.6% lipias) = 1,843 

Normalized BCF (at 1.0% lipids) = 242.5 

Food Chain Multiplier = 1.3 

J 

77 

"_ - - ". " 

" " . .. . . . " . . . - 
> Predicted BAF based on Log P and measured 

predicted HHBAF that 

is (242.5) (5.0) (1.3) 

BCF : 

BCF % L Norm BCF Reference 

(1.0% Ll 

7,899 

5,000 

7,144 

2,400 

2,300 

6,600 

8.45 

2.9 

7.28 

3 -79 

5 

5 

934.8 

981.3 

633.2 

1724. 

460. 

1320. 

Oliver and Niimi 1983 

Ban,erjee et al. 1984 

Oliver and Niimi 1985 

Carlson and Kosian 1987 

Van Hoogen and Opperhuiten 1988 

Scrap and Opperhuizen 1990 

Geometric mean normalized BCF = 920.4 

> Measured Bioaccumulation Factors: 

(based on field data for fish at trophic level 4) 

BAF % L Nom BAF Species Reference 

(1.0% L) 

- - 2,513 L. trout Oliver and Nicol 1982 

10,000 7.9 1,266 R. trout Oliver and Niimi 1983 

7,700 8 9 62 Salmon 

12,857 11 1,169 Salmon Oliver and Niimi 1988 . 

Oliver and Niimi 1985 

Geometric mean normalized BAF = 1,375 

(CAS#: 95-94-31 

> Predicted Brh: based on Log P: 

The following values were found for Log P: 

4.82 MedChem Star 

4.52 Hammers et al. 1982 

4.51 Miller et al. 1985 

4 -50 Bobra et al. 1985 

4.67 Isnard and Lambert 1989 

4.99 MedCh-em Calc 

78 
4.604 . de Bruijn et al. 1989 

Values ranging from 4.51 to 5.16 were cited by Hammers et 

al. (1982) and de Bruijn et al. (1989). A value of 4.7 was 

selected as a 'typical Log P', from which the following 

were obtained: 

Predicted BCF (at 7.6% lipids) = 2,056 

Normalized BCF (at 1.0% lipids) = 270.5 

Food Chain Multiplier = 1.4 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical 'Log P' is (270.5) (5.0) (1.4) 

= 1,894. 

> Predicted BAF based on Log P and measured BCF: 

. BCF %L Norm BCF Reference 

(1.0% L) 

8,301 8.45 982.4 Oliver and Niimi 1983 

4,050 8.5 476.5 Smith et al. 1990 

Geometric mean normalized BCF = 684.-2 

For 5.0% lipids at trophic level 4 the predicted HHBw that 

is calculated from the geometric mean normalized BCF is 

(684.2) (5.0) (1.4) = 4,789. 

> Measured Bioaccudation Factors: 

(based on field data for fish at trophic leirel 4) 

BAF % L Norm BAF Species Reference 

(1.0% L) 

- - 1513. L. trout Ol'iver and Nicol 1982 

s., 000 7.9 632.9 R. trout Oliver and Niimi 1983 . 

Geometric mean normal.ized BAF = 978.6 

For 5.0% lipids at trophic level 4 the predicted HHBAF is 

(978.6) (5.0) = 4,893. 

1,1,2,2 -TETRACHLOROETHANE [CAS#: 79-34-51 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

2.39 MedChem Star 

2.644 MedChem Calc 

2 -39 Isnard and Lambert 1989 . 

A value of 2.52 was selected as a 'typical Log P', from 

79 
which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 39.0 

Normalized BCF (at 1.0% lipids) = 5-13 

Food Chain Multiplier = 1.0 

For 5.0% lipids.at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (5.13) (5.0) (1.0) 

= 26. 

> Predicted BAF based on Log P and measured BCF: 

BCF % L Nom BCF Reference 

(1.0% L) 

8 4.8 1.67 Barrows et 'al. 1980 

Geometric mean normalized BCF = 1.67 

For 5.0% lipids at trophic level 4 the predicted HHBAF that. 

is calculated from the geometric mean normalized BCF is 

(1.67) (5.0) (1.0) = 8.4. 

Note: This BCF was based on uptake of radioactivity with no verification of the parent chemical. Thus this BCF 

and the resulting predicted HHBAF might be too high. 

> No measured BAF was found. 

TETRACHLOROETHYLENE. [CAS#: 127-18-41 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

3.40 MedChem Star 

3.02 MedChem Calc 

2.88 Isnard and Lambert 1989 

A value of 3.15 was selected as a 'typical Log P', from 

which the following were obtained: 

Predicted BCF (at 7..6% lipids) = 122.6 

Normalized BCF (at 1.0% lipids) = 16.1 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the "typical Log P' is (16.1) (5.0) (1.0) 

= 80. 

> Predicted BAF based on Log P ,and measured BCF: 

BCF %L Nom BCF Reference 

(1.0% L) 

80 
49 4.8 10.2 Barrows et al. 1980 

Geometric mean normalized BCF = 10.2 

Note: This BCF was based on uptake of radioactivity with no 

verification of the parent chemical. Thus this BCF 

and the resulting predicted HHBAF might be too high. 

> No measured BAF was found. 

THALLIUM [CAS#: 7440-28-01 

Barrows et al. (1980) reported a BCF of 34 based on whole-body 

measurements with the bluegill, whereas Zitko et al. (1975) 

obtain a BCF of 131 for muscle.of Atlant'ic salmon. The 

geometric mean of 34 and 131 is 67, which will be used as the 

predicted HHBAF for thallium. 

TOLUENE [CAS#: 108-88-31 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

2.70 MedChem Star 

2.79 MedChem Calc 

2.78 Hammers t al. .1982 

2.78 Burkhard et al. 1985 

2.786 de Bruijn'et al. 1989 

2.63 Isnard and Lambert 1989 

Values ranging-from 2.11 to 3.06 were cited by Eadsforth 

and Moser (19831, de Bruijn et al. (19891, and Brooke et 

al. (1990). A value of 2.76 was selected as a 'typical Log 

P', from which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 60.3 

Normalized BCF (at 1.,0% lipids) = 7.93 

Food Chain Multiplier = 1.0 

.. 2.65 Miller et al. 1985 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the "typical Log P' is (7.93) (5.0) (1.0) = 40. - 

> Predicted BAF based on Log P and measured BCF: 

81 
BCF % L Norm BCF Reference 

(1.0% L) 

1,250 4.04 309.4 Brooke and Call, ms. 

Geometric mean normalized BCF = 309.4 

Note: This BCF was based on uptake of radioactivity with no 

verification of the parent chemical. Thus this BCF 

and the resulting predicted HHBAF might be too high. 

> No measured BAF was found. 

TOXAPHENE [CAS#: 8001-35-21 

>' Predicted BAF based on Log P: 

The following values were found for Log P: 

4.33 MedChem Calc 

5.50 Isnard and Lambert 1989 

A value of 5.0 was selected as a 'typical Log P'8 from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 38548 

Normalized BCF (at 1.0% lipids) = 466.8 

Food Chain Multiplier = 2.6 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (466.8) (-5.0) (2.6) 

= 6,068. 

> Predicted BAF based on Log P and measured BCF: 

BCF %L 

19,465 4.78 

27,539 1.2 

8,427 4.76 

26,5,52 4.76 

62,462 4.1 

70,743 3.2 

19,532 4.76 

= juveniles ** = adults 

Norm BCF 

(1.0% L) 

4,073** 

2 , 633* 

15,235** 

22,107** 

22, 949* 

5,57a* 

4#103* 

Reference 

Mayer et al. 1977 

Schimmel et al. 1977 

Goodman et al. 1978 

Goodman 1986 

Goodman 1986 

Goodman 1986 

Goodman 1986 

- 

82 
Geometric mean normalized BCF = 4,,073 

.. 

> Measured Bioaccumulation Factors: 

(based on field data for fish at trophic level 4) 

. BV %L Norm BAF Species Reference 

(1.0% L) 

11,000,000 13.1 839,695 L. trout Swain et al. 1986 

1;772,727 8.3 213,582 L. trout Swain et al. 1986 

Geometric mean normalized BAF = 423,490 

For 5.0% lipids at trophic level 4 the predicted HHBAF is 

(423,490) (5.0) = 2,117,450. 

Note: Both of the measured BAFs are much larger than both 

of the predicted BUS. 

1,2,4-"RICHLOROBENZENE [CAS#: 120-82-11 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

3.98 MedChem Star 

4.02 MedChem Star 

4.28 MedChem Calc 

3.96 Hammers et al. 1982 

3.98 Miller et al. 1985 

4.10 Bobra et al. 1985 

4.04 Isnard and Lambert 1989 

4.05 de Bruijn et al. 1989 

Values ranging from 3.93 to 4.40 were cited by Hammers et 

al. (1982), Eadsforth and Moser (19831, and de Bruijn et 

al. (1989) . A value of 4.OCwas selected as a ".typical Log , pa, from which the following here-obtained: 

Predicted BCF (at 7.6% lipids) = 575.4 

Normalized BCF (at 1.0% lipids) = 75.7 

Food Chain Multiplier = 1.0 

83 
For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the 'typical Log P' is (75.7)(5.0)(1.0) 

= 378. 

> Predicted BAF based.on Log P and measured BCF: 

BCF 

2,800 

182 

1,606 

' 85 

349 

39 

2,040 

2,917 

124 

248 

914 

769 

769 

1,127 

1,365 

1,442 

991 

410 

2 , 026 

498 

%L 

7.6 

4.8 

9.12 

2.1 (e) 

3.2 (h) 

0.7 (a) 

8.45 

7.28 

1.8 

2.2 

4.4 

5.0 

5.2 

5.2 ' 

5.7 

5.8 

7.7 

8.2 

3.79 

11.4. 

Norm BCF 

(1.0% Ll 

368.4 

37.9 

.1-75.4 

40.5 . 

109.1 

55.7 

241.4 

400.7 

68.9 

112.7 

113.2 

182.8 

147.9 

147.9 

197.7 

235.3 

187.3 

120.9 

108.2 

177.7 

Reference 

Veith et al. 1979b . 

Macek et al. 1979; Barrows et . al. 1980, 

Kosian et al. 1981 

Galassi and Calamari 1983 

Galassi and Calamari 1983. 

Galassi and Calamari 1983 , 

Oliver and Niimi 1983 

Oliver and Niimi 1985 

Geyer et al. 1985 

Geyer et al. 1985 

Geyer et al. 1985 

Geyer et al. 1985 

Geyer et al. 1985 

Geyer et al. 1985 

Geyer et al. 1985 

Geyer et al. 1985 

Geyer et al. 1985 

Geyer ,et al. 1985 

Carlson and Kosian 1987 

Smith et al. 1990 

Geometric mean normalized BCF = 135.2 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is ca.lculated from the geometric mean normalized BCF'is 

(135.2) (5.0) (1.0) = 676. 

Note: The BCF reported by Macek et al. (1979) and Barrows 

et al. (1980) was based on uptake of radioactivity 

with no verification of the parent chemical and might 

be too high. 

> Measured Bioaccumulation Factors: 

(based on field data for fish at trophic level 4) 

BAF % L Norm BAF Species Reference 

(1.0% L) " 

. 

- - 245 L. trout .Oliver and Nicol 1982 

1,000 7.9 127 R. trout Oliver and Niimi 1983 

84 
1,200 8 150 Salmon Oliver and Niimi 1985 

Geometric mean normalized BAF = 167.1 

1,1, I-TRICHLOROETHANE [CAS#: 71-55-61 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

2.49 MedChem Star 

2.48 MedChem Calc 

'2.47 Isnard and Lambert 1989 

.A value of 2.48 was selected as a 'typical Log*P., from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 36.2 

Normalized BCF (at 1.0% lipids) = 4.76 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the-predicted HHBAF that'. 

is calculated from the "typical Log P' is (4.76)(5.0)(1.0) 

= 24. 

> Predicted BAF based on Log P and measured BCF: 

BCF %L Norm BCF ' Reference 

(1.0% Ll 

9 4.8 . 1.88 Barrows etal. 1980 

Geometric mean normalized BCF = 1.88 

For 5-03 lipids at trophic level 4 the predicted MBAF that 

is calculated from the geometric mean normalized' BCF is

(1.88) (5.0) (1.0) = 9.4. 

Note: This BCF was based on uptake of radioactivity with no 

verification of the parent chemical. Thus this BCF 

and the resulting predicted HHBAF might be too high. 

> No measured BAF was found. 

1,1,2 "I'RICHLOROETHANE [CAS#: 79-00-51 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

a5 
1.89 MedChem Star 

2 .05 MedChem Calc 

2.42 Isnard and Lambert 1989 ' 

Acvalue of 2.1 was selected as a 'typical Log P', from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 18.2 

Normalized BCF (at 1.0% lipids) = 2.39 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBm that 

is calculated from the 'typical Log P' is (2.39) (5.0) (1.0) 

= 12. 

> No measured BCF or BAF was found. 

TRICHLOROETHYLENE [CAS#: 79-01-61 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

2.42 MedChem Star 

2.267 MedChem Calc 

2.53 Miller et al.1985 

2.53 Isnard and Lambert 1989 

A value of 2.43 was selected as a 'typical Log P., from 

which the following were obtained: 

Predicted BCF (at 7.6% lipids) = 33.1 

Normalized BCF (at 1.0% lipids) = 4.36 

Food Chain Multiplier = 1.0 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the "typical Log P" is (4.36) (5.0) (1.0) 

= 22. 

> Predicted BAF based on Log P and measured BCF: 

BCF %L Norm BCF Reference 

(1.0% L) 

17 4.8 3.54 Barrows et al.1980 

Geometric 'mean normalized BCF = 3.54 

For 5.0% lipids at trophic level 4 the predicted HHBAF that 

is calculated from the geometric mean normalized BCF is' 

(3.54) (5.0) (1.0) = 18. 

Note: This BCF was based qn'u'ptake of ,radioactivity with no 

verification of the parent chemical. Thus this BCF 

and the resulting predicted HHBAF might be too high. 

86 
> No measured BAF was found. 

2,4,6"TRICHLOROPHENOL [CAS#: 88-06-21 

> Predicted BAF based on Log P: 

The following values were found for Log P: 

3.69 MedChem Star 

2.97 Isnard and L&,ert 1989 

3.57 MedChqn Calc 

A value of 3.5 was selected as a 'typical Log P', from 

which' the'following were obtained: 

Predicted BCF (at 7.6% lipids) = 231.7 

Normalized BCF (at 1.0% lipids) = 30.5 

Food Chain Multiplier = 1.0 

'For 5.0% lipids at trophic level. 4 the predicted HHBAF that 

is calculated from the 'typical.Log P9 is (30.5)-(5.01(1.0) . 

= 152. 

> Predicted BAF based on Log :P and measured BCF: 

BCF %L Norm BCF Reference 

(1.0% L) 

aa 12.4 7.10 Smith et al. 1990,1991 

Geometric mean normalized BCF = 7.10 

> No measured BAF was found. . 

VINYL CHLORIDE [CAS#: 75-01-41 

> Predicted BAF based on Log P: 

The following value was found for Log P: 

A value of.1.36 was selected as a "typical Log P', from 

which the following were obtained: 

1.36 MedChem Calc 

Predicted BCF (at 7.6% lipids) = 4.72 

Normalized BCF (at 3.0% lipids) = 0.621 

Food Chain Multiplier = l.0 

For 5.0% lipids at trophic' level 4 the predicted HHBAF that 

is calculated from the "typical Log P' is (0.621) (5.0) (1.0) 

= 3.1. 

a7 
> No measured BCF or BAF was found. 

- ZINC [CAS#: 7440-66-61 

Data presented'& Murphy et al. 11978) can.be used to 

calculate BAFs of 2.45 and 8.03 based on increased 

concentrations of zinc in water and in muscle-of bluegills and 

bass. The geometric mean of 2.45 and 8.03 is 4.4, which will 

be used as the predicted HHBAF for zinc. 
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98 
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