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                            Funding & Awards
 Identification and Analysis of Body Fluid Traces Using ATR FT-IR Spectroscopy
Note:
              This awardee has received supplemental funding. This award detail page includes information about both the original award and supplemental awards.
Award Information
Awardee
              RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK, THE
Award #
              2017-R2-CX-0006
Funding Category
              Competitive Discretionary
Location
                    ALBANY, NY
Congressional District
              20
Status
              Closed
Funding First Awarded
              2017
Total funding (to date)
              $149,983
Original Solicitation
              NIJ FY17 Graduate Research Fellowship Program in Science, Technology, Engineering and Mathematics
Description of original award (Fiscal Year 2017, $49,993)
Biological samples are of an extreme importance on a crime scene. They are the only one type of evidences that can provide information about a direct donor. Most of the presumptive tests, which could specify whether or not a stain is a biological trace, require chemical treatment and therefore are destructive to the sample. Thus, a method which is nondestructive to the stain is desired and could eliminate non-informative traces and allow to focus on most valuable pieces of evidence.
Tests confirming blood human origin are also mostly destructive to the sample and require laboratory settings. The ultimate goal of the proposed research project is to develop a new method for nondestructive, inexpensive, rapid, and on-scene detection, identification and characterization of body fluid traces in a forensic context.
An attenuated total reflection (ATR) Fourier transform-infrared (FT-IR) spectroscopy was already found to be easy-to-use, nondestructive to the sample, requiring no to minimum sample preparation, and highly selective as any vibrational spectroscopic methods. Moreover, portable instruments are commercially available to perform all analysis on-field. The (bio)chemical  composition of different body fluids was found to vary between them, and moreover, the composition of blood was proven to have quantitative changes within the same components between different species and different human donors.
This study will include development of spectroscopic library and statistical models for identification of dry traces of five main body fluids, development and validation of statistical models for differentiating human and animal blood, and development of spectroscopic library and statistical models for determining donors sex and race based on a dry bloodstain.
All samples will be chosen with gender, race, and age diversity for all aims regarding the human donors. Different breeds with mixed gender will be used for study involving discrimination between human and animal blood samples.
After spectra will be collected using FT-IR spectroscopy, they will be loaded into statistical software for preprocessing and modeling for discrimination purposes. To strengthen the reliability and validity of the analysis, validation tests will be performed using internal and external operations of predictions, with new and/or unknown body fluid samples.
ca/ncf
Supplemental Award 1 (Fiscal Year 2018, $49,993)
Biological samples are of an extreme importance on a crime scene. They are the only one type of evidences that can provide information about a direct donor. Most of the presumptive tests, which could specify whether or not a stain is a biological trace, require chemical treatment and therefore are destructive to the sample. Thus, a method which is nondestructive to the stain is desired and could eliminate non-informative traces and allow to focus on most valuable pieces of evidence.
Tests confirming blood human origin are also mostly destructive to the sample and require laboratory settings. The ultimate goal of the proposed research project is to develop a new method for nondestructive, inexpensive, rapid, and on-scene detection, identification and characterization of body fluid traces in a forensic context.
An attenuated total reflection (ATR) Fourier transform-infrared (FT-IR) spectroscopy was already found to be easy-to-use, nondestructive to the sample, requiring no to minimum sample preparation, and highly selective as any vibrational spectroscopic methods. Moreover, portable instruments are commercially available to perform all analysis on-field. The (bio)chemical  composition of different body fluids was found to vary between them, and moreover, the composition of blood was proven to have quantitative changes within the same components between different species and different human donors.
This study will include development of spectroscopic library and statistical models for identification of dry traces of five main body fluids, development and validation of statistical models for differentiating human and animal blood, and development of spectroscopic library and statistical models for determining donors sex and race based on a dry bloodstain.
All samples will be chosen with gender, race, and age diversity for all aims regarding the human donors. Different breeds with mixed gender will be used for study involving discrimination between human and animal blood samples.
After spectra will be collected using FT-IR spectroscopy, they will be loaded into statistical software for preprocessing and modeling for discrimination purposes. To strengthen the reliability and validity of the analysis, validation tests will be performed using internal and external operations of predictions, with new and/or unknown body fluid samples.
ca/ncf
    Supplemental Award 2 (Fiscal Year 2019, $49,997)
Biological samples are of an extreme importance on a crime scene. They are the only one type of evidences that can provide information about a direct donor. Most of the presumptive tests, which could specify whether or not a stain is a biological trace, require chemical treatment and therefore are destructive to the sample. Thus, a method which is nondestructive to the stain is desired and could eliminate non-informative traces and allow to focus on most valuable pieces of evidence.
Tests confirming blood human origin are also mostly destructive to the sample and require laboratory settings. The ultimate goal of the proposed research project is to develop a new method for nondestructive, inexpensive, rapid, and on-scene detection, identification and characterization of body fluid traces in a forensic context.
An attenuated total reflection (ATR) Fourier transform-infrared (FT-IR) spectroscopy was already found to be easy-to-use, nondestructive to the sample, requiring no to minimum sample preparation, and highly selective as any vibrational spectroscopic methods. Moreover, portable instruments are commercially available to perform all analysis on-field. The (bio)chemical  composition of different body fluids was found to vary between them, and moreover, the composition of blood was proven to have quantitative changes within the same components between different species and different human donors.
This study will include development of spectroscopic library and statistical models for identification of dry traces of five main body fluids, development and validation of statistical models for differentiating human and animal blood, and development of spectroscopic library and statistical models for determining donors sex and race based on a dry bloodstain.
All samples will be chosen with gender, race, and age diversity for all aims regarding the human donors. Different breeds with mixed gender will be used for study involving discrimination between human and animal blood samples.
After spectra will be collected using FT-IR spectroscopy, they will be loaded into statistical software for preprocessing and modeling for discrimination purposes. To strengthen the reliability and validity of the analysis, validation tests will be performed using internal and external operations of predictions, with new and/or unknown body fluid samples.
ca/ncf


  
Grant-Funded Publications
        
                      Discrimination between human and animal blood by attenuated total reflection Fourier transform-infrared spectroscopy
                  Differentiating smokers and nonsmokers based on Raman spectroscopy of oral fluid and advanced statistics for forensic applications
                  Forensic Phenotype Profiling Based on the Attenuated Total Reflection Fourier Transform-Infrared Spectroscopy of Blood: Chronological Age of the Donor
                  Raman spectroscopy for forensic semen identification: Method validation vs. environmental interferences
                  Phenotype profiling for forensic purposes: Nondestructive potentially on scene attenuated total reflection Fourier transform-infrared (ATR FT-IR) spectroscopy of bloodstains
                  Phenotype Profiling for Forensic Purposes: Determining Donor Sex Based on Fourier Transform Infrared Spectroscopy of Urine Traces
                  Toward Locard's Exchange Principle: Recent Developments in Forensic Trace Evidence Analysis
                  FT-IR Spectroscopy for Identification of Biological Stains for Forensic Purposes

  
Date Created: September 22, 2017
    
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