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                            Funding & Awards
 Rapid and selective extraction of male DNA from rape kits and other forensic evidence using pressure cycling
Award Information
Awardee
              Florida International University
Award #
              2011-NE-BX-K550
Funding Category
              Competitive
Location
                    Miami, FL
Awardee County
              Dade
Congressional District
              26
Status
              Closed
Funding First Awarded
              2011
Total funding (to date)
              $349,130
Original Solicitation
              NIJ FY 11 Applied Research and Development in Forensic Science for Criminal Justice Purposes
Description of original award (Fiscal Year 2011, $349,130)
The goal of this research is to reduce one bottleneck in the processing of samples: the long and complex process of separation and differential extraction of sperm cells in the presence of female DNA. This will be accomplished by the development of a novel and highly selective pressure-based DNA extraction procedure that is designed to burst open and extract DNA from male sperm cells while leaving the excess female cells in rape kits or other mixed forensic stains unaffected.

The pressure processing will be coupled with specific reducing agents such as TCEP and DTT which selectively attack sperm cells without damaging female epithelial cells. Using this technique, researchers believe that they can greatly simplify the long and complex processing of rape kits and other mixed forensic samples by selectively extracting the assailant's sperm cells, while leaving the victim's DNA behind.

ca/ncf



  
Grant-Funded Publications
        
                      Rapid and Selective Extraction of Male DNA from Rape Kits and Other Forensic Evidence Using Pressure Cycling
                  Genotyping Horse Epithelial Cells From Fecal Matter by Isolation of Polymerase Chain Reaction Products
                  Pressure-Based Alkaline Lysis With Immunocapture, a Method for Enhanced Recovery in Differential Extraction
                  The application of alkaline lysis and pressure cycling technology in the differential extraction of DNA from sperm and epithelial cells recovered from cotton swabs

  
Date Created: September 19, 2011
    
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