U.S. flag
      An official website of the United States government, Department of Justice.
        Here's how you know
      Here's how you know**Official websites use .gov**
            
            A **.gov** website belongs to an official government organization in the United States.
      **Secure .gov websites use HTTPS**
            
            A **lock** (
            A locked padlock
            ) or **https://** means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites. Menu

      
        close

            
            
                                              About NIJ
        
                    
  
          About NIJ
      
    
          The NIJ Director
          How NIJ Is Organized
          Mission and Guiding Principles
          Research and Development Process
          Strategic Framework
          Assessments of NIJ
          Annual Reports & Reports to Congress
          Staff Directory
          Funding & Awards
        
                    
  
          Funding & Awards
      
    
          Current Funding
          Forthcoming Funding
          Expired Funding
          Awards: Listing of Funded Projects
          Guidance for Applicants and Awardees
          Fellowship & Student Programs
          Travel Scholarships
          NIJ Challenge Program
          Library & Multimedia
        
                    
  
          Library & Multimedia
      
    
          NIJ Journal
          Podcasts
          Publications Listing
          Multimedia Listing
          Rated Practices on CrimeSolutions
          Rated Programs on CrimeSolutions
          Software & Databases
          Datasets
          Patents
          Topics
        
                    
  
          Topics
      
    
          Article Listing
          Corrections
          Courts
          Crime Prevention
          Crimes
          Drugs and Crime
          Equipment and Technology
          Forensic Sciences
          Youth Justice
          Justice System Reform
          Law Enforcement
          Tribal Crime and Justice
          Victims of Crime
          Events
        
                    
  
          Events
      
    
          NIJ 2024 Research Conference
          Scheduled Events
          On-Demand Events
          Research Meetings & Workshops
          NIJ-Hosted Online Training

                            
                                About
          Contact Us
          Subscribe
          Topics A-Z
                  Share
FacebookXLinkedInRedditPinterestEmail Home
                            Funding & Awards
 Development and Testing of a Rapid Multiplex Assay for the Identification of Biological Stains
Award Information
Awardee
              Colorado Seminary, owns & operates the University of Denver
Award #
              2011-CD-BX-0205
Funding Category
              Competitive
Location
                    Denver, CO
Congressional District
              1
Status
              Closed
Funding First Awarded
              2011
Total funding (to date)
              $24,986
Original Solicitation
              NIJ FY 11 Ph.D. Graduate Research Fellowship Program
Description of original award (Fiscal Year 2011, $24,986)
The Principal Investigator, Kevin M. Legg, will develop and rigorously test a faster, more accurate, and more sensitive multiplex assay for the simultaneous identification of saliva, semen, and vaginal secretions.  This assay will be developed using a triple quadruple mass spectrometer (QQQ) utilizing the multiple reaction monitoring (MRM) technique.  This technique uses a two-fold ion-filtration method where target peptides and their subsequent fragmentation ions are accurately detected even against the complex background of non-target molecules present in any body fluid.  The researcher will evaluate and select optimal ions for use in high-specificity MRM biomarker assays from human saliva, semen and vaginal secretions.  He will develop and optimize a multiplex QQQ_MRM mass-spectrometry assay for saliva, semen and vaginal secretions using single source and mixed reference samples.  Finally, he will assess the accuracy, reproducibility, and sensitivity of the assay using developmental validation and casework-type samples.
This work has the potential to significantly improve the accuracy and sensitivity of forensic serological testing.  It will provide practitioners with greatly improved tests for saliva and seminal fluid while also enabling the identification of vaginal secretions for which there are currently no accurate tests.  The multiplex design will eliminate the need to perform separate tests on an unknown stain.
ca/ncf



  
Grant-Funded Publications
        
                      Development and Testing of a Rapid Multiplex Assay for the Identification of Biological Stains

  
Date Created: September 8, 2011
    
          Side navigation
  

        

  
  
              Current Funding
    
      
            
            
            
      Forthcoming Funding
    
      
            
            
            
      Expired Funding
    
      
            
            
            
      Awards: Listing of Funded Projects
    
      
            
            
            
      Guidance for Applicants and Awardees
    
      
            
            
            
      Fellowship & Student Programs
    
      
            
            
            
      Travel Scholarships
    
      
            
            
            
      NIJ Challenge ProgramSimilar Awards
        
                      A Process, Adaptation, and Outcome Evaluation of San Gabriel Valley Crisis Assistance Response & Engagement (SGV CARE)
                  Impact of Relic DNA on Forensic Microbiome Applications in Criminal Investigations
                  The Low-Down on Methamphetamine Isomers: Prevalence and Pharmacology in Humans
 Top

      National Institute of Justice

        
        
                        About NIJ
                    About NIJ
      
    
                  How NIJ Is Organized
      
    
                  NIJ Director
      
    
                  Research Disclaimer
      
    
                  Funding & Awards
                    Funding & Awards Home
      
    
                  Awards Listing
      
    
                  Current Funding
      
    
                  Guidance for Applicants and Awardees
      
    
                  Resources
                    Events
      
    
                  Podcasts
      
    
                  Publications
      
    
                  Topics
      
    
                  Multimedia
      
    
                  Reuse Policy
      
    
                  Contacts
                    Contact NIJ
      
    
                  Staff Directory
      
    
                  Submit a FOIA Request
      
    
                  File a Civil Rights ComplaintFacebook
          
                      X
          
                      YouTube
  

      810 7th St. NW, Washington, D.C. 20531
      
              Secondary Footer link menu
            Accessibility
              Plain Language
              Privacy Policy
              Legal Policies and Disclaimer
              No FEAR Act
              Freedom of Information Act
              USA.gov
              Justice.gov