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                            Funding & Awards
 The Behavior of Contamination and Methods for Extraction and Capture of Low Copy Number and Degraded DNA.
Award Information
Awardee
              Washington State University Pullman
Award #
              2011-DN-BX-K549
Funding Category
              Competitive
Location
                    Pullman, WA
Congressional District
              5
Status
              Closed
Funding First Awarded
              2011
Total funding (to date)
              $249,867
Original Solicitation
              NIJ FY 11 Applied Research and Development in Forensic Science for Criminal Justice Purposes
Description of original award (Fiscal Year 2011, $249,867)
This purpose of this research is to test and evaluate three different 'DNA capture' methods for enriching CODIS marker DNA in LCN and degraded samples. Using a novel synthesized standard methodology the researchers will directly evaluate the efficiency of each method by determining the copy number originally entering the process and the number of copies retained at the end of the protocol.  In this way, the performance of the methods will be determined relative to complete recovery of DNA.  In addition, evaluation of the three capture methods will assess their respective abilities in removing PCR inhibitors from DNA extractions.  Lastly, each method will be applied to capturing CODIS markers from approximately 500 year old human remains. The goal of this project is to identify methods that hold promise for more reliably recovering and typing CODIS profiles from LCN and degraded samples. ca/ncf



  
Grant-Funded Publications
        
                      Evaluating the Efficiency of Primer Extension Capture as a Method to Enrich DNA Extractions
                  Are We Fishing or Catching? Evaluating the Efficiency of Bait Capture of CODIS Fragments
                  Bait and Capture: Holding on to Molecules of Interest
                  How Much DNA Is Lost? Measuring DNA Loss of Short-Tandem-Repeat Length Fragments Targeted by the PowerPlex 16 (R) System Using the Qiagen MinElute Purification Kit

  
Date Created: September 19, 2011
    
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