### SAT Report PMN Number: P-09-0451 SAT Date: 7/7/2009 Print Date: 3/17/2010

Related cases : CBI : Yes Health related cases: Ecotox related cases:

Concern levels : Type of Concern: Health Eco Comments Level of Concern: 1-2 3

Persistence Bioaccum Toxicity Comments 2 1 1
Exposure Based Review : Health: N o Ecotox: N o
Routes of exposure : Health: Inhalation only Ecotox: All releases to water Fate: ;
Keywords : Keywords: MUTA, IRR-E, AQUATOX-A,C
Summary of Assessment :

Fate : Fate Summary: P-09-0451 FATE: Solid with MP = Dec. > 198 EC (M) log Kow = 2.90 (M); S = 0.0198 mg/L at 25 EC (M) VP < 1.0E-6 torr at 25 EC (E) BP > 400 EC (E) H < 1.00E-8 (E) log Koc = 2.79 (E) log Fish BCF = 1.00 (E) log Fish BAF = 0.847 (E) POTW removal (%) = 0-90 via sorption and possible partial biodeg; OECD301B(Mod Sturm CO2 ev): 0%/28d. OECD111(Hydrolysis):t1/2( p H4,7):>1yr. Time for complete ultimate aerobic biodeg = moTime for primary anaerobic biodeg = da-wk Sorption to soils/sediments = moderate - strong PBT Potential: P2B1 *CEB FATE: Migration to ground water = negl due to and sorption

Health : Health Summary: Absorption is nil all routes based on physical/chemical p roperties. Based on the submitted studies, there is concern for mutagenicity and eye irritation, The PMN material is a moderate eye irritant and is positive in Salmonella and E coli with and without activation (no Prival modification). Low moderate concern. Test Data: p ositive in Salmonella with and without activation p ositive for chromosome aberrations in CHL cells negative in an oral mouse micronucleus assay not a dermal irritant, yellow staining at site of application moderate eye irritant, all effects cleared by day 8 acute oral study in rats - no deaths at 2000 or 5000 mg/kg, hunched posture and piloerection acute dermal study in rats - no deaths at 5000 mg/kg not a dermal sensitizer in the mouse local lymph node assay

Ecotox :

Test Organism Test Type Test End Point Predicted Measured Comments

fish 96-h LC50 7.5 >0.20 study data: N OEC=0.2; p redictions: or * daphnid 48-h LC50 2.2 >0.17 green algal 96-h EC50 0.52 0.0085 fish

_ chronic value 0.044 daphnid

_ chronic value 0.047 algal

_ chronic value 0.41 Sewage Sludge 3-h EC50

_ Sewage Sludge

_ Chronic Value

_ Ecotox Values Comments: Predictions are based on SARs for ; SAR chemical class = MW 521; solid with mp = 198 °C with decomposition (M); log Kow = 2.90 (M), 3.20 (EPI); S = 0.02 mg/L at 20 °C (P); p H7; effective concentrations based on 100% active ingredients and mean measured concentrations; hardness <150.0 mg/L as CaCO3; and TOC <2.0 mg/L;
This PMN was accompanied by three acute ecotoxicity studies. Analysis details are provided in the attached spreadsheet. Conclusions are repeated below.
Conclusion: The guidance document on aquatic toxicity testing of difficult to test substances (OECD series on testing and assessment number 23) was consulted for these tests. The stock solution was prepared by sonication and then stirred for 47 hours for the algal test and 45 hoursfor the Daphnia and fish tests. Some undissolved test material still remained and was thus filtered with a 0.45 μm membrane filter. The test concentrations for the algal test were measured at the start of the test and after 24 hours of exposure and decreased significantly (from 70-90 %) during this time frame but remained stable until 72 hours. Measured concentrations decreased from 0.29 mg/L to 0.22 mg/L after 24-hr exposure and then decreased again to 0.15 mg/L after 96 hours for the fish test. Time Weight Average concentrations were calculated to correspond to the concentrations in the test chambers for both the algal and fish tests. Measured values were used to determine the 48-hr EC50 for the Daphnia test because recovery of the test concentrations was between 70 and 105% of nominal. All three tests are considered valid. From the most sensitive species, algae, the chronic CC is derived from the EyC50 of 0.0085 mg/L for yield. Dividing this by an acute to chronic ratio of 4 to derive a simulated chronic value, gives 0.0021 mg/L. Dividing this value by an assessment (uncertainty) factor of 10 results in a CC of 0.0002, which rounds up to 0.001 mg/L or 1 μ g/L ( p pb).

Factors Values Comments Assessment Factor 10 Concentration of Concern ( p pb) 1

SARs SAR Class

Ecotox Factors Comments:

SAT Chair: Becky Jones