# Patent

## Title
Propeptide-luciferase fusion proteins and methods of use thereof

## Abstract

The present invention provides nucleic acid constructs that encode fusion peptides comprising a bioluminescent protein and a precursor of a secreted peptide or protein expressed at the cell surface and high throughput screening assays using same.

## Background
RELATED APPLICATION
This application is a U.S. National Phase application of International application No. PCT/US2012/063982 filed on Nov. 7, 2012, which claims the benefit of, priority to U.S. provisional application No. 61/556,619 filed on Nov. 7, 2011 and U.S. provisional application No. 61/576,530 filed on Dec. 16, 2011, the contents of each of which are incorporated herein by references in its entireties.


INCORPORATION-BY-REFERENCE OF SEQUENCE LISTING
The contents of the text file named “BRDI-016_N01US_ST25.txt”, which was created on Oct. 3, 2014 and is 717 KB in size, are hereby incorporated by reference in their entireties.
FIELD OF THE INVENTION
The present invention provides methods and compositions relating to detecting peptide secretion or cell surface expression of a peptide by a cell.
BACKGROUND OF THE INVENTION
Peptide hormones, cytokines and neuropeptides are signaling molecules that play key roles in normal physiology and disease states. Traditional immunoassays for these secreted proteins, such as the enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay, have enabled limited investigation into the pathways regulating their secretion, yet these assays are too expensive and time-consuming to be useful for large-scale chemical and genetic screening. Thus, a need exists for a high-throughput method of tracking peptide secretion or cell surface expression of a peptide.
A particular secreted peptide of interest is insulin. Failure to maintain adequate insulin secretion is central to the pathogenesis of both type 1 and type 2 diabetes. Determining the genetic pathways that regulate insulin secretion and finding small molecule probes of these pathways would greatly advance our understanding of the beta cell and bring us closer to a cure for both forms of diabetes. However, high throughput screens of insulin secretion using genetic (e.g., RNAi) or chemical perturbations are currently impracticable due to the lack of an amenable assay for measuring secreted insulin. Insulin ELISA kits and radioimmunoassays are not well suited to this application due to their expense, complicated handling requirements and restriction to 96-well format. Thus, a need exists for a high-throughput method of tracking peptide secretion, in particular, insulin hormone secretion.
SUMMARY OF THE INVENTION
The invention is based upon the discovery of methods and compositions relating to detecting peptide secretion from a cell or peptide expression at the cell surface, including but not limited to peptide hormones, cytokines and neuropeptides.
In one aspect the invention provides a nucleic acid construct encoding a fusion protein comprising a peptide hormone or peptide precursor linked to a bioluminescent protein, wherein the bioluminescent protein is flanked by two cleavage sites such that wherein the fusion protein is expressed by a cell the bioluminescent protein is cleaved from the peptide precursor and the bioluminescent protein is secreted simultaneously with the secretion or cell surface expression of the mature form of the propeptide. The propeptide is a prohormone, a preprohormone, a cytokine precursor or a neuropeptide precursor.
Exemplary propeptides that can be used in the present invention include, but are not limited to, the precursor proteins of amylin, insulin, glucagon (includes GRPP, glucagon, GLP-1, GLP-2), peptide YY, neuropeptide Y, pancreatic polypeptide, somatostatin, growth hormone-releasing hormone (GHRH), proopiomelanocortin (POMC, including ACTH, MSH), oxytocin, vasopressin-neurophysin-2, gonadotropin-releasing hormone (GnRH), thyroid-stimulating hormone, beta subunit (TSHB), cortisol-releasing factor (CRF), atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), renin, galanin, orexin, ghrelin-obestatin, cholecystokinin, gastrin, protachykinin-1 (substance P, neurokinin A, neuropeptide K, neuropeptide gamma), proenkephalin-A, proenkephalin-B, insulin-like growth hormone 1 (IGF-1), insulin-like growth hormone 2 (IGF-2), parathyroid hormone (PTH), parathyroid hormone-related protein (PTHrP), osteocalcin, urocortin-3, urocortin-2, urocortin-1, fibroblast growth factor 23 (FGF23), interleukin-1-beta (IL1B), tumor necrosis factor (TNF), interferon-gamma (IFNG), sortilin (SORT1), neuropeptide W, cocaine and amphetamine-related transcript (CART), transforming growth factor-beta-1 (TGFB1), transforming growth factor-beta-2 (TGFB2), transforming growth factor-beta-3 (TGFB3), platelet-derived growth factor-alpha (PDGFA), brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), albumin, calcitonin, cortistatin (CORT), ADAM-10, -11, -12, -15, -17, -22, -23, -28, -33, ADAMTS-1, -3-10, -12 through -20, BMP-1 through -6, -10, MMP-11, -14 through -17, -21, -23, -24, -25, -28, beta-defensins and mesothelin.
Preferably, the peptide precursor is encoded by any one of the nucleotide sequences of SEQ ID NOs: 5-100. Optionally the nucleic acid construct contains a promoter.
In some embodiments the peptide precursor is preproinsulin and the bioluminescent protein is within the C-peptide component of preproinsulin. In other embodiments, the peptide precursor is preproamylin and the N-terminal end of the bioluminescent protein is inserted between the regions encoding the signal peptide and the mature amylin hormone or between the mature amylin hormone and the C-terminus of the molecule. In another embodiment, the peptide precursor is preproglucagon and the bioluminescent protein is inserted between the regions encoding the GRPP and glucagon, glucagon and GLP-1, or GLP-1 and GLP-2. The nucleic acid construct further comprises an additional cleavage site at the N-terminal and C-terminal ends of the bioluminescent protein. Optionally, there is an additional cleavage site at the C-terminal end of the bioluminescent protein. Optionally, there are at least 3 additional nucleotides flanking at least one cleavage site to enhance cleavage.
Exemplary cleavage sites that can be used for the constructs of the present invention include, but are not limited to, aagagg (SEQ ID NO: 101); aagcgt (SEQ ID NO: 102); aagcgc (SEQ ID NO: 103); aaaaga (SEQ ID NO: 104); aagaggagg (SEQ ID NO: 105); aagaga (SEQ ID NO: 106); cgcaaa (SEQ ID NO: 107); cggcgg (SEQ ID NO: 108); tatctg (SEQ ID NO: 109); aggcgg (SEQ ID NO: 110); cggagc (SEQ ID NO: 111); cggtct (SEQ ID NO: 112); cgaagc (SEQ ID NO: 113); aaacgg (SEQ ID NO: 114); aagagaggt (SEQ ID NO: 115); aaacgaggc (SEQ ID NO: 116); gggccgccgc (SEQ ID NO: 117); ccacgagct (SEQ ID NO: 118); ccccgagct (SEQ ID NO: 119); cgaaggcagctgcgggct (SEQ ID NO: 120); cgtaggcagctgagggta (SEQ ID NO: 121); cgccgcagt (SEQ ID NO: 122); cgaaga (SEQ ID NO: 123); cggaga (SEQ ID NO: 124); agaagg (SEQ ID NO: 125); aaacgc (SEQ ID NO: 126); tccagcattcggagg (SEQ ID NO: 127); agcagtcggagg (SEQ ID NO: 128); gagagggac (SEQ ID NO: 129); cggcgc (SEQ ID NO: 130); aggcgc (SEQ ID NO: 131); cggggcaccaag (SEQ ID NO: 132); caccgc (SEQ ID NO: 133); acccgtgtc (SEQ ID NO: 134); tcgcgcatt (SEQ ID NO: 135); cgacgg (SEQ ID NO: 136); aagagaaga (SEQ ID NO: 137); and ggccgccgc (SEQ ID NO: 138).
The bioluminescent protein is luciferase, such as for example, Gaussia luciferase or Cypridina luciferase. In some embodiments, the bioluminescent protein is truncated, for example, the Gaussia or the Cypridina luciferase lacks a native signal sequence. The invention also includes a cell comprising a nucleic acid construct of the invention and a fusion protein encoded by the nucleic acid construct of the invention. The cell is capable of expressing the encoded fusion protein. The cell is, for example, a pancreatic cell, an immune cell, a neuron, a hepatocyte, a myocyte, a kidney cell, an adipocyte, an osteocyte or a cell line derived therefrom. The cell is for example, a peptide-secreting cell such as a beta cell, an alpha cell, an L-cell, a K-cell, a neuron or a cell line derived therefrom. The cell is for example, an immune cell such as a B cell, a T cell, a monocyte, a macrophage, a dendritic cell, a mast cell, a neutrophil or a cell line derived therefrom. Alternatively, the cell is an embryonic stem cell or an iPS cell. The invention also includes a cell comprising at least two nucleic acid constructs of the invention, wherein the cell is capable of expressing the encoded fusion proteins and wherein the bioluminescent proteins of the nucleic acid constructs are different. For example, the first nucleic acid construct comprises Gaussia luciferase and the second nucleic acid construct comprises Cypridina luciferase. Optionally, the cell also comprises a control nucleic acid construct encoding a control luciferase different from the luciferase of the fusion proteins to be used as an internal reference.
The invention further provides an assay for screening for compounds that modulate peptide secretion or cell surface expression of a peptide by a cell by contacting the cell culture comprising the cell of the invention with a test compound and determining bioluminescence in the cell culture. A difference in bioluminescence from the cell culture that has been contacted with the test compound compared to bioluminescence from a control cell culture indicates that the test compound modulates peptide secretion or expression at the cell surface.
The invention further provides an assay for screening for compounds that modulate peptide secretion or cell surface expression of the peptide bu a cell by contacting the cell culture comprising the cell of the invention with a test compound, separating the population of cells into single cells, and determining bioluminescence in the cell culture. A difference in bioluminescence from the cell culture that has been contacted with the test compound compared to bioluminescence from a control cell culture indicates that the test compound modulates peptide secretion or cell surface expression. The separation of the population of cells into single cells can be performed by, for example, a microfluidic device. In some embodiments, the test compound is a test nucleic acid and further comprising determining the identity of the test nucleic acid using single cell nucleic acid amplification methods.
The invention also provides a method of screening for compounds that differentiate embryonic stem cells or induced pluripotent stem (iPS) cells into mature cell types of interest, such as pancreatic beta cells, by contacting a cell culture comprising the cells of the invention with a test compound and determining bioluminescence in the cell culture. An increase in bioluminescence from the cell culture that has been contacted with the test compound compared to bioluminescence from a control cell culture indicates that the test compound induced differentiation of embryonic stem cells or iPS cells into the cell type of interest.
The invention also provides a method of screening for compounds that differentiate embryonic stem cells or iPS cells into glucose responsive beta cells by contacting a cell culture comprising the cells of the invention with a test compound and determining bioluminescence in the cell culture. An increase of bioluminescence from the cell culture that has been contacted with the test compound compared to bioluminescence from a control cell culture indicates that the test compound differentiates embryonic stem cells or iPS cells into glucose responsive beta cells.
The test compound is for example a nucleic acid or a small molecule. The nucleic acid is for example an RNAi or gene overexpression sequence.
The present invention also provides a nucleic acid expression vector comprising a nucleic acid sequence encoding a bioluminescent protein, wherein the bioluminescent protein lacks a native signal peptide; a nucleic acid sequence encoding two cleavage sites, wherein the cleavage sites flank the bioluminescent protein such that when the vector is expressed by a cell, the bioluminescent protein is cleaved from the remaining peptide; and at least one insertion site for insertion of a nucleic acid sequence encoding a propeptide such that the inserted nucleic acid sequence is in-frame with the bioluminescent protein. The insertion site is a restriction enzyme site, multiple cloning site containing multiple restriction enzyme sites, or a site recognized by a recombinase. Optionally, the nucleic acid expression vector comprises a promoter, wherein the promoter is operatively linked to the nucleic acid sequence encoding the bioluminescent protein. Optionally, the nucleic acid expression vector comprises a selective marker operatively linked to a second promoter. The selective marker can be an antibiotic resistance gene, drug resistance gene, toxin resistance gene or a cell surface marker.
Alternatively, a nucleic acid expression vector may comprise any nucleic acid construct described herein operatively linked to a promoter and a selective marker operatively linked to a second promoter.
The present invention further provides a kit. The kit includes any or at least one of the nucleic acid expression vectors described herein, at least one luciferase substrate and instructions for use. The luciferase substrate will be selected according to the nucleic acid expression vector of the kit, such that the luciferase of the expression vector will dictate the luciferase substrate included in the kit. The present invention further provides a kit that contains any one of the cells that express a propeptide-luciferase fusion protein as described herein. Any kit of the present invention further comprises a control nucleic acid construct that encodes a control luciferase or cells expressing the control luciferase for use as an internal control.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Although methods and materials similar or equivalent to those described herein can be used in the practice of the present invention, suitable methods and materials are described below. All publications, patent applications, patents, and other references mentioned herein are expressly incorporated by reference in their entirety. In cases of conflict, the present specification, including definitions, will control. In addition, the materials, methods, and examples described herein are illustrative only and are not intended to be limiting.
Other features and advantages of the invention will be apparent from and encompassed by the following detailed description and claims.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 depicts the structure of the preproamylin-Gaussia luciferase (PAG) construct and its expression in MIN6 cells. (A) The luciferase was placed near the C-terminal end of the proamylin peptide, adjacent to an existing “prohormone convertase 2” (PC2) cleavage site. An additional PC2 cleavage site was inserted to the 3′-end of the luciferase sequence. s.p. stands for signal peptide. (B) Immunohistochemistry of MIN6 cells expressing preproamylin-luciferase reporter showing co-localization of luciferase and insulin.
FIG. 2 is two graphs showing the validation of proamylin-luciferase reporter in MIN6 cells. Luciferase activity tracks closely with insulin concentration (as measured by ELISA) during glucose stimulation (R2=0.96).
FIG. 3 is two schematics illustrating (A) wildtype MIN6 beta cells and (B) “PAG” containing MIN6 beta cells. Luciferase travels within proamylin from ER, through the Golgi and to the secretory vesicles, where it is cleaved out and co-secreted with endogenous insulin and amylin.
FIG. 4 is a graph showing the pre-proamylin-luciferase reporter in MIN6 cells responds as predicted to known insulin secretagogues. Cells were treated for 1 hour at the indicated glucose concentrations with either DMSO, 25 uM glipizide, 2.5 uM glucokinase activator (GKA), 50 uM 3-isobutyl-1-methylxanthine (IBMX), 20 uM forskolin, 40 mM potassium chloride (KCl), or 20 uM phorbol myristate acetate (PMA).
FIG. 5 depicts the structure of proglucagon-Gaussia luciferase (G2) construct. The luciferase was placed between the Glp-1 and the Glp-2 peptides; flanked by PC1 and PC2 cleavage sites on the N and C-terminal ends. s.p. stands for signal peptide.
FIG. 6 is a graph establishing that the secreted luciferase serves as a close proxy for secreted glucagon. The G2 construct was transfected into mouse alpha cells. Luciferase secretion was measured by luciferase activity in response to stimulation with arginine or KCl and varying dosages of glucose (DMSO was used as control).
FIG. 7 is two graphs establishing that the secreted luciferase serves as a close proxy for secreted Glp-1. The G2 construct was transfected into mouse L cells. A) Luciferase secretion was measured by luciferase activity in response to stimulation with forskolin/IBMX, KCl, glipizide, PMA or L-glutamine. B) The correlation of luciferase secretion (signal) with secreted Glp-1 concentration was very high.
FIG. 8 shows characterization of the proinsulin-luciferase fusion protein. (A) Diagram of proinsulin-luciferase fusion construct, showing Gaussia luciferase within the C-peptide portion of proinsulin. The natural secretory signal peptide of Gaussia luciferase was removed to prevent unregulated secretion; the luciferase sequence was flanked with recognition sites for prohormone convertase 2 (PC2) to minimize carryover of extraneous amino acids after processing within the secretory vesicles. (B) Immunohistochemistry of INS-1E cells expressing the proinsulin-luciferase reporter, stained for luciferase (green, left), insulin (red, middle), or both (merged, right). (C) Glucose-stimulated secretion of luciferase (red) and insulin (blue) from MIN6 cells expressing the fusion construct (left). Correlation between insulin concentration and luciferase activity (right). (D) Assessment of luciferase secretion induced by known insulin secretagogues. Cells were treated for 1 hour at the indicated glucose concentrations with either DMSO, 25 uM glipizide, 2.5 uM glucokinase activator (GKA), 20 mM arginine, 1 nM glucagon-like peptide-1 (GLP-1), 50 uM 3-isobutyl-1-methylxanthine (IBMX), 20 uM forskolin, 40 mM potassium chloride (KCl), or 20 uM phorbol myristate acetate (PMA). Correlation between insulin and luciferase secretion for all samples (right).
FIG. 9 shows the application of proinsulin-luciferase reporter to high-throughput chemical screen. (A) Scatter plot of the results of high-throughput screening of 1600 compounds for luciferase secretion using MIN6 cells expressing proinsulin-luciferase construct. The screen was performed in duplicate in the presence of sub-stimulatory 100 mg/dL glucose, and Z-scores were calculated based on the DMSO control distribution. DMSO controls shown in red, glipizide in green, KCl in yellow, test compounds in blue. (B) Insulin secretion induced by the top-scoring compounds in MIN6 cells in the presence of sub-stimulatory 100 mg/dL glucose, as measured using a standard insulin ELISA. (C) Effect of same eight compounds on insulin secretion from dissociated human islets, in the presence of sub-stimulatory 50 mg/dL glucose. (D) Scatter plot comparing results of high-throughput screening for luciferase secretion in presence and absence of glucose. DMSO controls shown in red, glipizide in green, glucokinase activator in yellow, test compounds in blue. (E) Measurement of insulin secretion induced by hit compounds in MIN6 cells, in presence (100 mg/dL, in red) and absence (0 mg/dL, in blue) of glucose. Data represent the average±standard deviation of 2 replicates.
FIG. 10 is a schematic depicting an assay to screen for beta cell differentiation from iPS cells using luciferase secretion as the readout as a marker for beta cell maturation.
FIG. 11 is a graph depicting the correlation between secreted luciferase and secreted insulin from proinsulin reporter-expressing human beta-like cells.



DETAILED DESCRIPTION OF THE INVENTION
The invention is based in part upon methods and compositions relating to detecting peptide secretion or cell surface expression of the peptide by a cell, including but not limited to that of peptide hormones, cytokines, neuropeptides and transmembrane proteins. Examples of such peptides include but are not limited to, insulin, amylin, glucagon, glucagon-like peptide-1 (GLP-1), glucagon-like peptide-2 (GLP-2), gastric inhibitory peptide (GIP), growth hormone (GH), proopiomelanocortin (POMC)-derived hormones, growth hormone releasing hormone (GHRH), parathyroid hormone (PTH), gonadotropin releasing hormone (GnRH), peptide YY, neuropeptide Y, protachykinin-1 (Substance P, Neurokinin A, Neuropeptide K, Neuropeptide gamma), proenkephalin-A, proenkephalin-B, oxytocin, vasopressin-neurophysin-2, thyroid-stimulating hormone, beta subunit (TSHB), cortisol-releasing factor (CRF), atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), renin, galanin, orexin, ghrelin-obestatin, cholecystokinin, gastrin, insulin-like growth hormone 1 (IGF-1), insulin-like growth hormone 2 (IGF-2), parathyroid hormone (PTH), parathyroid hormone-related protein (PTHrP), osteocalcin, urocortin-3, urocortin-2, urocortin-1, fibroblast growth factor 23 (FGF23), interleukin-1-beta (IL1B), tumor necrosis factor (TNF), interferon-gamma (IFNG), sortilin (SORT1), neuropeptide W, cocaine and amphetamine-related transcript (CART), transforming growth factor-beta-1 (TGFB1), transforming growth factor-beta-2 (TGFB2), transforming growth factor-beta-3 (TGFB3), platelet-derived growth factor-alpha (PDGFA), brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), albumin, calcitonin, cortistatin (CORT), ADAM-10, -11, -12, -15, -17, -22, -23, -28, -33, ADAMTS-1, -3-10, -12 through -20, BMP-1 through -6, -10, MMP-11, -14 through -17, -21, -23, -24, -25, -28, beta-defensins and mesothelin. The methods are useful in screening for compounds or genes that modulate peptide secretion or cell surface expression. Specifically, the invention provides for the high throughput measurement of peptide secretion or cell surface expression in the setting of genetic and chemical perturbations, and as such is well suited to screens for genes and compounds impacting physiologic processes. Additionally, the methods are useful in screening for compounds that are capable of differentiating one cell type (e.g., embryonic stem cells or iPS cells) into mature cell type, such as a peptide-secreting cell type (e.g., glucose-responsive pancreatic beta cell).
The present invention provides novel tools and methods for measuring peptide secretion or expression at the cell surface. In some embodiments, the peptide is a hormone peptide, prohormone, or precursor thereof. In some embodiments, the peptide is a neuropeptide or precursor thereof. In other embodiments, the peptide is a cytokine or precursor thereof. Specifically, the present invention provides a fusion protein comprising a propeptide and a bioluminescent protein, nucleic acid constructs encoding the fusion proteins, vectors and host cells containing the nucleic acid constructs. The fusion gene constructs may also contain promoters and other transcriptional and/or translational regulatory sequences to drive expression of the fusion gene.
Propeptide Fusion Protein Constructs
Fusion proteins comprise a single continuous linear polymer of amino acids which comprise the full or partial sequence of two or more distinct proteins. The construction of fusion proteins is well-known in the art. Two or more amino acids sequences may be joined chemically, for instance, through the intermediacy of a crosslinking agent. In a preferred embodiment, a fusion protein is generated by expression of a fusion gene construct in a cell. A fusion gene construct comprises a single continuous linear polymer of nucleotides which encodes the full or partial sequences of two or more distinct proteins. Fusion gene constructs of the present invention contain a reporter gene in addition to other genes or fragments thereof that encode at least one propeptide. Fusion gene constructs generally also contain replication origins active in eukaryotic and/or prokaryotic cells and one or more selectable markers encoding, for example, drug resistance. They may also contain viral packaging signals as well as transcriptional and/or translational regulatory sequences and RNA processing signals.
Reporter genes for use in the invention encode detectable proteins, include, but are by no means limited to, Luciferase, Thalassicolin, Aequorin, Mitrocomin, Clytin (synonomous with Phialidin, Obelin, Mnemiopsin) and Berovin. For example, the detectable protein is a bioluminescent protein. An exemplary bioluminescent protein is the Gaussia luciferase, which is utilized for its unique properties of being small in size and with increased luminescence in comparison to other more commonly used luciferases (1000 times brighter than Firefly or Renilla luciferase). Another exemplary bioluminescent protein is the Cypridina luciferase. In some embodiments, the bioluminescent protein is truncated or a fragment thereof such that the bioluminescent protein retains its luminescent characteristics. The bioluminescent protein may have a native signal peptide that controls its secretion. Preferably, the bioluminescent protein lacks the native signal peptide. An exemplary modified luciferase is the Gaussia luciferase wherein the luciferase lacks the N-terminal signal peptide that controls the secretion of the luciferase (e.g., SEQ ID NO: 1). An exemplary modified luciferase is the Cypridina luciferase wherein the luciferase lacks the N-terminal signal peptide (e.g., SEQ ID NO: 3).
The configuration of the propeptide fusion proteins of the present invention is unique. Early studies and attempts for hormone-reporter fusion proteins have had limited success. Early hormone-reporter constructs caused varying levels of toxicity to the host cells. Other constructs were improperly processed, for example the fusion proteins were sequestered in the endoplasmic reticulum or other secretory pathway organelles rather than appropriately secreted. Other prohormone reporters were constructed such that the mature hormone was fused to the reporter protein, which could potentially result in misfolding and retention of the fusion proteins, as well as alteration of the function and activity of the mature hormone. As described above, these early hormone-reporter fusion proteins had limitations in their use for accurately tracking hormone secretion and amenability for high throughput screening assays. The fusion proteins of the present invention were specifically designed to address the difficulties and limitations of the previous generation of hormone-receptor fusion proteins.
Three modifications were made to an early preproinsulin fusion protein to reduce toxicity to host cells and increase accuracy of secretion for peptide tracking. (1) The bioluminescent protein utilized is small, comprised of less than 200 amino acids. For example, the bioluminescent protein is a Gaussia luciferase. The Gaussia luciferase is additionally modified such that it is lacking its native secretion signal peptide. (2) The prohormone gene of the fusion protein and the host cell are of the same species. The early preproinsulin fusion protein utilized a human preproinsulin gene and was expressed in a mouse cell line. It would be well within the knowledge of the skilled artisan to use the present disclosure to construct propeptide fusion proteins for expression in mouse, rat, or hamster pancreatic cell lines (i.e., beta-cell lines) with mouse, rat or hamster prohormone genes, which are known in the art. Similarly, the skilled artisan would be able to construct a human prohormone fusion protein using the present disclosure for expression in a human pancreatic cell line (i.e., beta-cell line) upon availability of said cell line. (3) Cleavage sites were introduced into the fusion protein such that there is minimal intervening sequence between the cleavage site and the bioluminescent protein that may interfere with proper processing. For example, for the proinsulin-luciferase fusion construct, no extra amino acids are present between the cleavage site and the bioluminescent protein, such that the luciferase will have no extra amino acids after it is cleaved out of proinsulin. Together, these modifications produced prohormone fusion proteins useful for tracking peptide hormone secretion, as described herein.
An important distinction between the fusion proteins of the present invention and other hormone reporter proteins is that the fusion protein of the present invention is processed normally to produce a mature hormone peptide that is fully intact, unmodified, does not contain additional amino acids, and therefore, should be fully functional. Furthermore, the mature hormone peptide is predicted to be fully competent for endogenous interactions, signaling, and function. The bioluminescent protein is processed and secreted simultaneously and in parallel to secretion of the mature hormone, thereby providing an accurate readout of secretion or expression at the cell surface of the mature hormone. In contrast, other hormone-reporter constructs irreversibly join the mature hormone with the bioluminescent or reporter protein, thereby significantly altering the processing, secretion and downstream function of both the mature hormone and the luciferase protein.
The inventors also discovered that this strategy is useful for other propeptides that are processed prior to secretion or cell surface expression. The nucleic acid constructs and fusion proteins described herein are useful for the tracking peptide secretion and expression at the cell surface.
The present invention provides fusion proteins that comprise a propeptide and a bioluminescent protein. The bioluminescent protein is inserted into the propeptide such that there is at least one cleavage site at the N-terminal end and at least one cleavage site at the C-terminal end of the bioluminescent protein. Any one of the cleavage sites can be native to the propeptide or can be introduced through recombinant DNA techniques known in the art. Exemplary cleavage sites for prohormone fusion proteins, include but are not limited to, prohormone convertase 1⁄3 (PC1⁄3), prohormone convertase 2 (PC2), and caspase-1 cleavage sites. Preferably, the cleavage sites are the same cleavages sites that are normally used to process the endogenous propeptide. Utilizing the same cleavage sites at the N and C-terminal ends of the bioluminescent protein that are normally used to process the propeptide allows the cleavage of the bioluminescent protein upon processing of the propeptide for secretion of the mature protein. Importantly, the cleaved bioluminescent protein is free (e.g., separated or non-covalently linked) from the mature peptide(s), and therefore, serves as an accurate readout of peptide secretion or expression of the peptide at the cell surface. In some embodiments, the cleavage sites can be selected and introduced by the skilled artisan such that the bioluminescent protein is cleaved only under specific circumstances determined by the skilled artisan (e.g., cellular context). For example, the skilled artisan could control fusion protein processing and bioluminescent protein secretion by introducing PC2 cleavage sites at the N and C-terminal ends of the bioluminescent protein and expressing the fusion protein in cells that only express PC2 at a certain time or under a specific condition.
The nucleic acid sequences listed in SEQ ID NO: 139 through SEQ ID NO: 330 encode fusion proteins in which a bioluminescent protein is incorporated into a propeptide, flanked by cleavage sites required for the maturation of the propeptide into a mature protein. Whereas the original propeptide cleavage site is maintained on the 5′ end of the bioluminescent protein in each fusion protein, an additional cleavage site is added at the 3′ end of the bioluminescent protein in each fusion protein to allow for release of the bioluminescent protein after proper cleavage. Both the native and added cleavage sites are underlined in the nucleic acid sequences. While most constructs were designed with the minimal required sequence (i.e., beginning of proprotein—cleavage site #1—bioluminescent protein—cleavage site #2—end of proprotein), in some cases extra flanking nucleotides were included around each cleavage site to create the optimal context for cleavage at these locations, as predicted by the available scientific literature on each protein.
These additional nucleotides encode additional amino acids that enhance cleavage at the cleavage site are referred to herein as “additional cleavage sequence” or ACS. Cleavage sites in which cleavage can be enhanced through additional amino acids can be readily determined by one of ordinary skill in the art through available scientific literature. Some cleavage sites do not require presence of an ACS. ACS can comprise 3 or more nucleotides, wherein the total number of nucleotides is a multiple of 3. For example, the ACS may be 3, 6, 9, 12, or more nucleotides. The ACS encodes amino acids that do not interfere with the function of the fusion protein. For example, the ACS encodes 1, 2, 3, 4, or more additional amino acids.
Optionally, restriction enzyme sites can be added on either side of the bioluminescent protein to allow for construction of the fusion protein. These restriction enzyme sites do not alter the function or expression of the bioluminescent protein and the propeptide.
The present invention provides a nucleic acid construct encoding a fusion protein comprising a peptide precursor linked to a bioluminescent protein, wherein the bioluminescent protein is flanked by two cleavage sites such that wherein the fusion protein is expressed by a cell the bioluminescent protein is cleaved from the peptide precursor and secreted simultaneously with the mature protein.
The present invention provides a nucleic acid construct encoding a fusion protein comprising a peptide precursor linked to a bioluminescent protein, wherein the bioluminescent protein is flanked by two cleavage sites such that wherein the fusion protein is expressed by a cell the bioluminescent protein is cleaved from the peptide precursor simultaneously upon expression of the mature peptide at the cell surface.
The present invention encompasses fusion proteins encoded by the nucleic acid constructs described herein. The fusion proteins have at least 70%, 75%, 80%, 85%, 90%, or 95% sequence identity to the fusion proteins encoded by the nucleic acid constructs of the present invention, such that the resulting fusion protein retains the ability to be cleaved to separate the bioluminescent protein and the mature peptide such that the bioluminescent protein is secreted and the mature form of the propeptide is either secreted or expressed on the cell surface. In some embodiments, the fusion proteins have at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to the fusion proteins encoded by the nucleic acid constructs of the present invention, such that the resulting fusion protein retains the ability to be cleaved to separate the bioluminescent protein and the mature peptide such that the bioluminescent protein is secreted and the mature form of the propeptide is either secreted or expressed on the cell surface. Additionally, the mature peptide produced by the fusion protein having 70%, 75%, 80%, 85%, 90%, or 95% sequence identity to the fusion proteins encoded by the nucleic acid constructs of the present invention has activities substantially similar to the native (e.g., endogenous) mature peptide.
The present invention provides a preproinsulin fusion protein. Preproinsulin consists of a B-peptide, a C-peptide, and an A-peptide (from N to C-terminus). The bioluminescent protein is inserted within the C-peptide component of preproinsulin. A native PC1⁄3 cleavage sites exists between the B-peptide and the C-peptide, and a native PC2 cleavage site exists between the C-peptide and the A-peptide. In some embodiments, the bioluminescent protein replaces all or some portion of the C-peptide. In some embodiments, the bioluminescent protein is flanked at the N and C terminus by the native cleavage cites. Optionally, an additional cleavage site is added at the N or C-terminal end of the bioluminescent protein. In some embodiments, the bioluminescent protein two cleavage sites are added at the N and C terminus of the bioluminescent protein, i.e., PC2 cleavage sites.
The present invention provides a preproamylin fusion protein. Native PC2 cleavage sites exist at the N and C-terminal ends of the mature amylin peptide. The bioluminescent protein is located at the C-terminus of the preproamylin, adjacent to the native prohormone convertase enzyme 2 (PC2) cleavage site at the C-terminus of the mature amylin peptide. Optionally, an additional cleavage site is added at the C-terminal end of the bioluminescent protein, i.e. PC2. Optionally, an additional cleavage site is inserted at the N and C-terminal end of the bioluminescent protein. Optionally, restriction enzyme sites can be added on either side of the bioluminescent protein to allow for construction of the fusion protein (e.g., aatgca in SEQ ID NO: 139 and SEQ ID NO: 140). These restriction sites are dispensable for the function of the fusion protein.
The present invention provides a preproglucagon fusion protein. The preproglucagon comprises four distinct peptides GRPP, glucagon, GLP-1 (glucagon-like protein 1), and GLP-2 (glucagon-like protein 2), in order from N to C-terminus. Native PC2 sites exist between GRPP and glucagon, two PC2 sites exist between glucagon and Glp-1, a PC1 site exists between glucagon and Glp-1 (in between the existing PC2 sites), and a PC1 site exists between Glp-1 and Glp-2. The bioluminescent protein is inserted between the GLP-1 and GLP-2 encoding regions. Optionally, an additional cleavage site is added at the C-terminal end of the bioluminescent protein, i.e., PC2. In some embodiments, the cellular context in which the preproglucagon fusion protein is expressed determines which peptide is tracked by the bioluminescent protein. In one embodiment, the preproglucagon fusion protein is expressed in L cells which express PC1 and therefore would cause cleavage of the Glp-1 peptide and the bioluminescent protein such that the luminescence would accurately reflect Glp-1 secretion. In one embodiment, the preproglucagon fusion protein is expressed in alpha cells which express PC2 and therefore would cause cleavage of the glucagon peptide and the bioluminescent protein such that the luminescence would accurately reflect glucagon secretion.
Additionally, the present invention provides a fusion protein comprising one propeptide selected from the group consisting of: peptide YY, neuropeptide Y, pancreatic polypeptide, somatostatin, growth hormone-releasing hormone (GHRH), proopiomelanocortin (POMC, including ACTH, MSH), oxytocin, vasopressin-neurophysin-2, gonadotropin-releasing hormone (GnRH), thyroid-stimulating hormone, beta subunit (TSHB), cortisol-releasing factor (CRF), atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), renin, galanin, orexin, ghrelin-obestatin, cholecystokinin, gastrin, protachykinin-1 (substance P, neurokinin A, neuropeptide K, neuropeptide gamma), proenkephalin-A, proenkephalin-B, insulin-like growth hormone 1 (IGF-1), insulin-like growth hormone 2 (IGF-2), parathyroid hormone (PTH), parathyroid hormone-related protein (PTHrP), osteocalcin, urocortin-3, urocortin-2, urocortin-1, fibroblast growth factor 23 (FGF23), interleukin-1-beta (IL1B), tumor necrosis factor (TNF), interferon-gamma (IFNG), sortilin (SORT1), neuropeptide W, cocaine and amphetamine-related transcript (CART), transforming growth factor-beta-1 (TGFB1), transforming growth factor-beta-2 (TGFB2), transforming growth factor-beta-3 (TGFB3), platelet-derived growth factor-alpha (PDGFA), brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), albumin, calcitonin, cortistatin (CORT), ADAM-10, -11, -12, -15, -17, -22, -23, -28, -33, ADAMTS-1, -3-10, -12 through -20, BMP-1 through -6, -10, MMP-11, -14 through -17, -21, -23, -24, -25, -28, beta-defensins and mesothelin.
The fusion gene constructs of the invention are introduced into cells to assay for peptide secretion. The fusion gene constructs may also contain promoters and other transcriptional and/or translational regulatory sequences that are normally associated with the gene encoding propeptide. The fusion gene constructs may be introduced into cells by any method of nucleic acid transfer known in the art, including, but not limited to, viral vectors, transformation, co-precipitation, electroporation, neutral or cationic liposome-mediated transfer, microinjection or gene gun. Viral vectors include retroviruses, poxviruses, herpes viruses, adenoviruses, and adeno-associated viruses. Particularly preferred in the present invention are retroviral vectors, which are capable of stable integration into the genome of the host cell. For example, retroviral constructs encoding integration and packaging signals, drug resistance markers and one or more fusion genes of interest are useful in the practice of the invention.
The fusion gene constructs or fusion proteins of the invention may be introduced into cultured cells, animal cells in vivo, animal cells ex vivo, or any other type of cell in which it is desired to study peptide secretion. Preferably, the fusion gene construct is introduced into a cell in which the peptide is secreted or a precursor cell thereof. For example, the fusion gene construct comprising preproinsulin or preproamylin is introduced into beta cells to measure insulin secretion. In some embodiments the beta cell is a beta cell line, such as the murine MIN6 beta cell line or the rat INS-1E beta cell line. The beta cell may be a mouse, rat or hamster beta cell. To measure glucagon or GLP-1 secretion, the fusion construct comprising preproglucagon is introduced into alpha cells or L-cells, respectively. Although a human beta cell line is not yet available in the art, the skilled artisan would be able to use and apply the methods described herein to expressing the fusion proteins of the invention in such a human beta cell line upon its availability.
The recombinant cells of the invention that express the fusion constructs of the invention provide for development of screening assays, particularly for high throughput screening of molecules that up- or down-regulate the activity of peptide secretion.
Exemplary luciferase nucleic acid sequences include, but are not limited to:








Gaussia luciferase without signal peptide or stop codon (nucleic acid sequence):



(SEQ ID NO: 1)



Aagcccaccgagaacaacgaagacttcaacatcgtggccgtggccagcaacttcgcgaccacggatctcgatgctgaccgcgggaa



 


gttgcccggcaagaagctgccgctggaggtgctcaaagagatggaagccaatgcccggaaagctggctgcaccaggggctgtctga


 


tctgcctgtcccacatcaagtgcacgcccaagatgaagaagttcatcccaggacgctgccacacctacgaaggcgacaaagagtccg


 


cacagggcggcataggcgaggcgatcgtcgacattcctgagattcctgggttcaaggacttggagcccatggagcagttcatcgcaca


 


ggtcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgccaacgtgcagtgttctgacctgctcaagaagtggctgccgc


 


aacgctgtgcgacctttgccagcaagatccagggccaggtggacaagatcaagggggccggtggtgac


 


Gaussia luciferase with signal peptide and stop codon (nucleic acid sequence):


(SEQ ID NO: 2)



Atgggagtcaaagttctgtttgccctgatctgcatcgctgtggccgaggccaagcccaccgagaacaacgaagacttcaacatcgtgg



 


ccgtggccagcaacttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaagaagctgccgctggaggtgctcaaa


 


gagatggaagccaatgcccggaaagctggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacgcccaagatgaag


 


aagttcatcccaggacgctgccacacctacgaaggcgacaaagagtccgcacagggcggcataggcgaggcgatcgtcgacattcc


 


tgagattcctgggttcaaggacttggagcccatggagcagttcatcgcacaggtcgatctgtgtgtggactgcacaactggctgcctcaa


 


agggcttgccaacgtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcgacctttgccagcaagatccagggccag


 


gtggacaagatcaagggggccggtggtgactaa


 


Cypridina luciferase without signal peptide or stop codon (nucleic acid sequence):


(SEQ ID NO: 3)



Tactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaa



 


tgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatg


 


tgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacat


 


acgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctg


 


accaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgacc


 


ctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgctgttgagatgccaggcttcaacatcaccgtcattgagttcttcaa


 


actgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtgga


 


gatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtc


 


cactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctacta


 


ctacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgct


 


gctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgca


 


aggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaa


 


agtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgta


 


cagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttc


 


aagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattct


 


tttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaata


 


gtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcct


 


gaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccaga


 


tgaatgcaaa


 


Cypridina luciferase with signal peptide and stop codon (nucleic acid sequence):


(SEQ ID NO: 4)



atgaagaccttaattcttgccgttgcattagtctactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagtt



 


ccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtga


 


aaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatgg


 


aaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctgga


 


gaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactga


 


gaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgc


 


caggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacag


 


caaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattca


 


gcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaag


 


gacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcaca


 


cgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgac


 


aaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacac


 


aggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattc


 


tggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcc


 


tacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcg


 


gtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaac


 


agaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgacc


 


gtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacata


 


aagcatggagacaccctagaagtaccagatgaatgcaaatag






Exemplary nucleic acid sequences of the propeptides of the present invention include, but are not limited to, those listed below.








Proamylin Mouse (nucleic acid sequence):



(SEQ ID NO: 5)



atgatgtgcatctccaaactgccagctgtcctcctcatcctctctgtggcactgaaccacttgagagctacacctgtcagaagtggtagca



 


accctcagatggacaaacggaagtgcaacacggccacgtgtgccacacaacgcctggcaaactttttggttcgttccagcaacaacctt


 


ggtccagtcctcccaccaaccaacgtgggatcgaatacatatggcaagaggaatgcggcaggggatccaaatagggaatccaggatt


 


tcttactcgtttaa


 


Proamylin Human (nucleic acid sequence):


(SEQ ID NO: 6)



atgggcatcctgaagctgcaagtatttctcattgtgctctctgttgcattgaaccatctgaaagctacacccattgaaagtcatcaggtggaa



 


aagcggaaatgcaacactgccacatgtgcaacgcagcgcctggcaaattttttagttcattccagcaacaactttggtgccattctctcatc


 


taccaacgtgggatccaatacatatggcaagaggaatgcagtagaggttttaaagagagagccactgaattacttgcccctttag


 


Proinsulin 2 Mouse (nucleic acid sequence):


(SEQ ID NO: 7)



atggccctgtggatgcgcttcctgcccctgctggccctgctcttcctctgggagtcccaccccacccaggcttttgtcaagcagcaccttt



 


gtggttcccacctggtggaggctctctacctggtgtgtggggagcgtggcttcttctacacacccatgtcccgccgtgaagtggaggac


 


ccacaagtggcacaactggagctgggtggaggcccgggagcaggtgaccttcttgaccttggcactggaggtggcccagcagaagc


 


gtggcattgtagatcagtgctgcaccagcatctgctccctctaccagctggagaactactgcaactag


 


Proinsulin Human (nucleic acid sequence):


(SEQ ID NO: 8)



atggccctgtggatgcgcctcctgcccctgctggcgctgctggccctctggggacctgacccagccgcagcctttgtgaaccaacacct



 


gtgcggctcacacctggtggaagctctctacctagtgtgcggggaacgaggcttcttctacacacccaagacccgccgggaggcaga


 


ggacctgcaggtggggcaggtggagctgggcgggggccctggtgcaggcagcctgcagcccttggccctggaggggtccctgca


 


gaagcgtggcattgtggaacaatgctgtaccagcatctgctccctctaccagctggagaactactgcaactag


 


Proglucagon (includes GRPP, glucagon, GLP-1, GLP-2) Mouse (nucleic acid sequence):


(SEQ ID NO: 9)



atgaagaccatttactttgtggctggattgcttataatgctggtgcaaggcagctggcagcacgcccttcaagacacagaggagaaccc



 


cagatcattcccagcttcccagacagaagcgcatgaggaccctgatgagatgaatgaagacaaacgccactcacagggcacattcac


 


cagcgactacagcaaatacctggactcccgccgtgcccaagattttgtgcagtggttgatgaacaccaagaggaaccggaacaacatt


 


gccaaacgtcatgatgaatttgagaggcatgctgaagggacctttaccagtgatgtgagttcttacttggagggccaggcagcaaagga


 


attcattgcttggctggtgaaaggccgaggaaggcgagacttcccagaagaagtcgccattgccgaggaactcggccgcaggcacgc


 


tgatggctccttctctgacgagatgagcaccattctggataatcttgccaccagggacttcatcaactggctgattcaaaccaagatcactg


 


acaagaaatag


 


Proglucagon (includes GRPP, glucagon, GLP-1, GLP-2) Human (nucleic acid sequence):


(SEQ ID NO: 10)



atgaaaagcatttactttgtggctggattatttgtaatgctggtacaaggcagctggcaacgttcccttcaagacacagaggagaaatcca



 


gatcattctcagcttcccaggcagacccactcagtgatcctgatcagatgaacgaggacaagcgccattcacagggcacattcaccagt


 


gactacagcaagtatctggactccaggcgtgcccaagattttgtgcagtggttgatgaataccaagaggaacaggaataacattgccaa


 


acgtcacgatgaatttgagagacatgctgaagggacctttaccagtgatgtaagttcttatttggaaggccaagctgccaaggaattcatt


 


gcttggctggtgaaaggccgaggaaggcgagatttcccagaagaggtcgccattgttgaagaacttggccgcagacatgctgatggtt


 


ctttctctgatgagatgaacaccattcttgataatcttgccgccagggactttataaactggttgattcagaccaaaatcactgacaggaaat


 


aa


 


Peptide YY Mouse (nucleic acid sequence):


(SEQ ID NO: 11)



atggtggcggtgcgcaggccttggcccgtcacggtcgcaatgctgctaatcctgctcgcctgtctgggagccctggtggacgcctacc



 


ctgccaaaccagaggctcccggcgaagacgcctccccggaggagctgagccgctactacgcctccctgcgccactacctcaacctg


 


gtcacccggcagcggtatggaaaaagagatgtccccgcagctctgttctccaaactgctcttcacagacgacagcgacagcgagaac


 


ctccccttcaggccagaaggtttggaccagtggtga


 


Peptide YY Human (nucleic acid sequence)


(SEQ ID NO: 12)



atggtgttcgtgcgcaggccgtggcccgccttgaccacagtgcttctggccctgctcgtctgcctaggggcgctggtcgacgcctaccc



 


catcaaacccgaggctcccggcgaagacgcctcgccggaggagctgaaccgctactacgcctccctgcgccactacctcaacctggt


 


caccggcagcggtatgggaaaagagacggcccggacacgcttctttccaaaacgttcttccccgacggcgaggaccgccccgtca


 


ggtcgcggtcggagggcccagacctgtggtga


 


Neuropeptide Y Mouse (nucleic acid sequence)


(SEQ ID NO: 13)



atgctaggtaacaagcgaatggggctgtgtggactgaccctcgctctatctctgctcgtgtgtttgggcattctggctgaggggtacccct



 


ccaagccggacaatccgggcgaggacgcgccagcagaggacatggccagatactactccgctctgcgacactacatcaatctcatca


 


ccagacagagatatggcaagagatccagccctgagacactgatttcagacctcttaatgaaggaaagcacagaaaacgcccccagaa


 


caaggcttgaagacccttccatgtggtga


 


Neuropeptide Y Human (nucleic acid sequence):


(SEQ ID NO: 14)



atgctaggtaacaagcgactggggctgtccggactgaccctcgccctgtccctgctcgtgtgcctgggtgcgctggccgaggcgtacc



 


cctccaagccggacaacccgggcgaggacgcaccagcggaggacatggccagatactactcggcgctgcgacactacatcaacct


 


catcaccaggcagagatatggaaaacgatccagcccagagacactgatttcagacctcttgatgagagaaagcacagaaaatgttccc


 


agaactcggcttgaagaccctgcaatgtggtga


 


Pancreatic polypeptide Mouse (nucleic acid sequence):


(SEQ ID NO: 15)



atggccgtcgcatactgctgcctctccctgtttctcgtatccacttgggtggctctgctgctgcagcccctgcaggggacctggggagcc



 


cccctggagccaatgtacccaggcgactatgcgacacctgagcagatggcacaatatgaaactcagctccgcagatacatcaacacac


 


tgaccaggcctaggtatgggaagagagccgaggaggagaacacaggtggacttcctggagtgcagctctccccctgcaccagcccc


 


ccagttggcttgattccctgctctgcgccctggagctga


 


Pancreatic polypeptide Human (nucleic acid sequence):


(SEQ ID NO: 16)



atggctgccgcacgcctctgcctctccctgctgctcctgtccacctgcgtggctctgttactacagccactgctgggtgcccagggagcc



 


ccactggagccagtgtacccaggggacaatgccacaccagagcagatggcccagtatgcagctgatctccgtagatacatcaacatg


 


ctgaccaggcctaggtatgggaaaagacacaaagaggacacgctggccttctcggagtgggggtccccgcatgctgctgtccccagg


 


gagctcagcccgctggacttataa


 


Somatostatin Mouse (nucleic acid sequence):


(SEQ ID NO: 17)



atgctgtcctgccgtctccagtgcgccctggctgcgctctgcatcgtcctggctttgggcggtgtcaccggcgcgccctcggaccccag



 


actccgtcagtttctgcagaagtctctggcggctgccaccgggaaacaggaactggccaagtacttcttggcagagctgctgtccgagc


 


ccaaccagacagagaatgatgccctggagcccgaggatttgccccaggcagctgagcaggacgagatgaggctggagctgcagag


 


gtctgccaactcgaacccagcaatggcaccccgggaacgcaaagctggctgcaagaacttcttctggaagacattcacatcctgttag


 


Somatostatin Human (nucleic acid sequence):


(SEQ ID NO: 18)



atgctgtcctgccgcctccagtgcgcgctggctgcgctgtccatcgtcctggccctgggctgtgtcaccggcgctccctcggaccccag



 


actcgtcagtttctgcagaagtccctggctgctgccgcggggaagcaggaactggccaagtacttcttggcagagctgctgtctgaac


 


ccaaccagacggagaatgatgccctggaacctgaagatctgtcccaggctgctgagcaggatgaaatgaggcttgagctgcagagat


 


ctgctaactcaaacccggctatggcaccccgagaacgcaaagctggctgcaagaatttcttctggaagactttcacatcctgttag


 


GHRH Mouse (nucleic acid sequence):


(SEQ ID NO: 19)



atgctgctctgggtgctctttgtgatcctcatcctcaccagtggctcccactgctcactgcccccctcacctcccttcaggatgcagcgaca



 


cgtagatgccatcttcaccaccaactacaggaaactcctgagccagctgtatgcccggaaagtgatccaggacatcatgaacaagcaa


 


ggggagaggatccaggaacaaagggccaggctcagccgccaggaagacagcatgtggacagaggacaagcagatgaccctgga


 


gagcatcttgcagggattcccaaggatgaagccttcagcggacgcttga


 


GHRH Human (nucleic acid sequence):


(SEQ ID NO: 20)



atgccactctgggtgttcttctttgtgatcctcaccctcagcaacagctcccactgctccccacctccccctttgaccctcaggatgcggcg



 


gtatgcagatgccatcttcaccaacagctaccggaaggtgctgggccagctgtccgcccgcaagctgctccaggacatcatgagcag


 


gcagcagggagagagcaaccaagagcgaggagcaagggcacggcttggtcgtcaggtagacagcatgtgggcagaacaaaagca


 


aatggaattggagagcatcctggtggccctgctgcagaagcacaggaactcccagggatga


 


POMC (ACTH, MSH) Mouse (nucleic acid sequence):


(SEQ ID NO: 21)



atgccgagattctgctacagtcgctcaggggccctgttgctggccctcctgcttcagacctccatagatgtgtggagctggtgcctggag



 


agcagccagtgccaggacctcaccacggagagcaacctgctggcttgcatccgggcttgcaaactcgacctctcgctggagacgccc


 


gtgtttcctggcaacggagatgaacagcccctgactgaaaacccccggaagtacgtcatgggtcacttccgctgggaccgcttcggcc


 


ccaggaacagcagcagtgctggcagcgcggcgcagaggcgtgcggaggaagaggcggtgtggggagatggcagtccagagccg


 


agtccacgcgagggcaagcgctcctactccatggagcacttccgctggggcaagccggtgggcaagaaacggcgcccggtgaagg


 


tgtaccccaacgttgctgagaacgagtcggcggaggcctttcccctagagttcaagagggagctggaaggcgagcggccattaggctt


 


ggagcaggtcctggagtccgacgcggagaaggacgacgggccctaccgggtggagcacttccgctggagcaacccgcccaagga


 


caagcgttacggtggcttcatgacctccgagaagagccagacgcccctggtgacgctcttcaagaacgccatcatcaagaacgcgca


 


caagaagggccagtga


 


POMC (ACTH, MSH) Human (nucleic acid sequence):


(SEQ ID NO: 22)



atgccgagatcgtgctgcagccgctcgggggccctgttgctggccttgctgcttcaggcctccatggaagtgcgtggctggtgcctgga



 


gagcagccagtgtcaggacctcaccacggaaagcaacctgctggagtgcatccgggcctgcaagcccgacctctcggccgagactc


 


ccatgttcccgggaaatggcgacgagcagcctctgaccgagaacccccggaagtacgtcatgggccacttccgctgggaccgattcg


 


gccgccgcaacagcagcagcagcggcagcagcggcgcagggcagaagcgcgaggacgtctcagcgggcgaagactgcggccc


 


gctgcctgagggcggccccgagccccgcagcgatggtgccaagccgggcccgcgcgagggcaagcgctcctactccatggagca


 


cttccgctggggcaagccggtgggcaagaagcggcgcccagtgaaggtgtaccctaacggcgccgaggacgagtcggccgaggc


 


cttccccctggagttcaagagggagctgactggccagcgactccgggagggagatggccccgacggccctgccgatgacggcgca


 


ggggcccaggccgacctggagcacagcctgctggtggcggccgagaagaaggacgagggcccctacaggatggagcacttccgc


 


tggggcagcccgcccaaggacaagcgctacggcggtttcatgacctccgagaagagccagacgcccctggtgacgctgttcaaaaa


 


cgccatcatcaagaacgcctacaagaagggcgagtga


 


Oxytocin Mouse (nucleic acid sequence):


(SEQ ID NO: 23)



atggcctgccccagtctcgcttgctgcctgcttggcttactggctctgacctcggcctgctacatccagaactgccccctgggcggcaag



 


agggctgtgctggacctggatatgcgcaagtgtctcccctgcggcccgggcggcaaaggacgctgcttcggaccaagcatctgctgc


 


gcggacgagctgggctgcttcgtgggcaccgccgaggcgctgcgctgccaggaggagaactacctgccttcgccctgccagtctgg


 


ccagaagccctgcgggagcggaggccgctgcgccgccacaggcatctgctgcagcccggatggctgccgcacagaccccgcctg


 


cgaccctgagtctgccttctcggagcgctga


 


Oxytocin Human (nucleic acid sequence):


(SEQ ID NO: 24)



atggccggccccagcctcgcttgctgtctgctcggcctcctggcgctgacctccgcctgctacatccagaactgccccctgggaggca



 


agagggccgcgccggacctcgacgtgcgcaagtgcctcccctgcggccccgggggcaaaggccgctgcttcgggcccaatatctg


 


ctgcgcggaagagctgggctgcttcgtgggcaccgccgaagcgctgcgctgccaggaggagaactacctgccgtcgccctgccagt


 


ccggccagaaggcgtgcgggagcgggggccgctgcgcggtcttgggcctctgctgcagcccggacggctgccacgccgaccctg


 


cctgcgacgcggaagccaccttctcccagcgctga


 


Vasopressin-Neurophysin-2 Mouse (nucleic acid sequence):


(SEQ ID NO: 25)



atgctcgccaggatgctcaacactacgctctccgcttgtttcctgagcctgctggccttctcctccgcctgctacttccagaactgcccaag



 


aggcggcaagagggccatctctgacatggagctgagacagtgtctcccctgcggcccgggcggcaaaggacgctgcttcggaccaa


 


gcatctgctgcgcggacgagctgggctgcttcgtgggcaccgccgaggcgctgcgctgccaggaggagaactacctgccctcgccc


 


tgccagtccggccagaagccctgcgggagcgggggccgctgcgccgccgtgggcatctgctgcagcgacgagagctgcgtggcc


 


gagcccgagtgccacgacggttttttccgcctcacccgcgctcgggagccaagcaacgccacacagctggacggccctgctcgggc


 


gctgctgctaaggctggtacagctggctgggacacgggagtccgtggattctgccaagccccgggtctactga


 


Vasopressin-Neurophysin-2 Human (nucleic acid sequence):


(SEQ ID NO: 26)



atgcctgacaccatgctgcccgcctgcttcctcggcctactggccttctcctccgcgtgctacttccagaactgcccgaggggcggcaa



 


gagggccatgtccgacctggagctgagacagtgcctcccctgcggccccgggggcaaaggccgctgcttcgggcccagcatctgct


 


gcgcggacgagctgggctgcttcgtgggcacggctgaggcgctgcgctgccaggaggagaactacctgccgtcgccctgccagtcc


 


ggccagaaggcgtgcgggagcgggggccgctgcgccgccttcggcgtttgctgcaacgacgagagctgcgtgaccgagcccgagt


 


gccgcgagggctttcaccgccgcgcccgcgccagcgaccggagcaacgccacgcagctggacgggccggccggggccttgctgc


 


tgcggctggtgcagctggccggggcgcccgagcccttcgagcccgcccagcccgacgcctactga


 


Gonadotropin-releasing hormone (GnRH) Mouse (nucleic acid sequence):


(SEQ ID NO: 27)



atgatcctcaaactgatggccggcattctactgctgactgtgtgtttggaaggctgctccagccagcactggtcctatgggttgcgccctg



 


ggggaaagagaaacactgaacacttggttgagtctttccaagagatgggcaaggaggtggatcaaatggcagaaccccagcacttcg


 


aatgtactgtccactggccccgttcacccctcagggatctgcgaggagctctggaaagtctgattgaagaggaagccaggcagaagaa


 


gatgtag


 


Gonadotropin-releasing hormone (GnRH) Human (nucleic acid sequence):


(SEQ ID NO: 28)



atgaagccaattcaaaaactcctagctggccttattctactgacttggtgcgtggaaggctgctccagccagcactggtcctatggactgc



 


gccctggaggaaagagagatgccgaaaatttgattgattctttccaagagatagtcaaagaggttggtcaactggcagaaacccaacgc


 


ttcgaatgcaccacgcaccagccacgttctcccctccgagacctgaaaggagctctggaaagtctgattgaagaggaaactgggcaga


 


agaagatttaa


 


Thyroid-stimulating hormone, beta subunit (TSHB) Mouse (nucleic acid sequence):


(SEQ ID NO: 29)



atgagtgctgccgtcctcctctccgtgctttttgctcttgcttgtgggcaagcagcatccttttgtattcccactgagtatacaatgtacgtgga



 


taggagagagtgtgcctactgcctgaccatcaacaccaccatctgtgctgggtattgtatgacacgggatatcaatggcaaactgtttcttc


 


ccaaatatgcactctctcaggatgtctgtacatacagagacttcatctacagaacggtggaaataccaggatgcccgcaccatgttactcc


 


ttatttctccttccctgtcgccataagctgcaagtgtggcaagtgtaatactgacaacagtgactgcatacacgaggctgtcagaaccaact


 


actgcaccaagccgcagtctttctatctggggggattttctgtttaa


 


Thyroid-stimulating hormone, beta subunit (TSHB) Human (nucleic acid sequence):


(SEQ ID NO: 30)



atgactgctctctttctgatgtccatgctttttggccttacatgtgggcaagcgatgtctttttgtattccaactgagtatacaatgcacatcgaa



 


aggagagagtgtgcttattgcctaaccatcaacaccaccatctgtgctggatattgtatgacacgggatatcaatggcaaactgtttcttcc


 


caaatatgctctgtcccaggatgtttgcacatatagagacttcatctacaggactgtagaaataccaggatgcccactccatgttgctccct


 


atttttcctatcctgttgctttaagctgtaagtgtggcaagtgcaatactgactatagtgactgcatacatgaagccatcaagacaaactactg


 


taccaaacctcagaagtcttatctggtaggattttctgtctaa


 


Cortisol-releasing factor (CRF) Mouse (nucleic acid sequence):


(SEQ ID NO: 31)



atgcggctgcggctgctggtgtccgcgggcatgctgctggtggctctgtcgtcctgcctgccttgcagggccctgctcagcaggggatc



 


cgtcccccgagcgccgcgggccccgcagcccttgaatttcttgcagccggagcagccccagcaacctcagccggttctgatccgcat


 


gggtgaagaatacttcctccgcctggggaatctcaacagaagtcccgctgctcggctgtcccccaactccacgcccctcaccgcgggt


 


cgcggcagccgcccctcgcacgaccaggctgcggctaactttttccgcgtgttgctgcagcagctgcagatgcctcagcgctcgctcg


 


acagccgcgcggagccggccgaacgcggcgccgaggatgccctcggtggccaccagggggcgctggagagggagaaggcggtc


 


ggaggagccgcccatctctctggatctcaccttccaccttctgcgggaagtcttggaaatggcccgggcagagcagttagctcagcaa


 


gctcacagcaacaggaaactgatggagattatcgggaaatga


 


Cortisol-releasing factor (CRF) Human (nucleic acid sequence):


(SEQ ID NO: 32)



atgcggctgccgctgcttgtgtccgcgggagtcctgctggtggctctcctgccctgcccgccatgcagggcgctcctgagccgcgggc



 


cggtcccgggagctcggcaggcgccgcagcaccctcagcccttggatttcttccagccgccgccgcagtccgagcagccccagcag


 


ccgcaggctcggccggtcctgctccgcatgggagaggagtacttcctccgcctggggaacctcaacaagagcccggccgctcccctt


 


tcgcccgcctcctcgctcctcgccggaggcagcggcagccgcccttcgccggaacaggcgaccgccaactttttccgcgtgttgctgc


 


agcagctgctgctgcctcggcgctcgctcgacagccccgcggctctcgcggagcgcggcgctaggaatgccctcggcggccacca


 


ggaggcaccggagagagaaaggcggtccgaggagcctcccatctccctggatctcaccttccacctcctccgggaagtcttggaaat


 


ggccagggccgagcagttagcacagcaagctcacagcaacaggaaactcatggagattattgggaaataa


 


Atrial natriuretic peptide (ANP) Mouse (nucleic acid sequence):


(SEQ ID NO: 33)



atgggctccttctccatcaccctgggcttcttcctcgtcttggccttttggcttccaggccatattggagcaaatcctgtgtacagtgcggtgt



 


ccaacacagatctgatggatttcaagaacctgctagaccacctggaggagaagatgccggtagaagatgaggtcatgcccccgcagg


 


ccctgagtgagcagactgaggaagcaggggccgcacttagctccctccccgaggtgcctccctggactggggaggtcaacccacct


 


ctgagagacggcagtgctctagggcgcagcccctgggacccctccgatagatctgccctcttgaaaagcaaactgagggctctgctcg


 


ctggccctcggagcctacgaagatccagctgcttcgggggtaggattgacaggattggagcccagagtggactaggctgcaacagctt


 


ccggtaccgaagataa


 


Atrial natriuretic peptide (ANP) Human (nucleic acid sequence):


(SEQ ID NO: 34)



atgagctccttctccaccaccaccgtgagcttcctccttttactggcattccagctcctaggtcagaccagagctaatcccatgtacaatgc



 


cgtgtccaacgcagacctgatggatttcaagaatttgctggaccatttggaagaaaagatgcctttagaagatgaggtcgtgcccccaca


 


agtgctcagtgagccgaatgaagaagcgggggctgctctcagccccctccctgaggtgcctccctggaccggggaagtcagcccag


 


cccagagagatggaggtgccctcgggcggggcccctgggactcctctgatcgatctgccctcctaaaaagcaagctgagggcgctgc


 


tcactgcccctcggagcctgcggagatccagctgcttcgggggcaggatggacaggattggagcccagagcggactgggctgtaac


 


agcttccggtactga


 


Brain natriuretic peptide (BNP) Mouse (nucleic acid sequence):


(SEQ ID NO: 35)



atggatctcctgaaggtgctgtcccagatgattctgtttctgcttttcctttatctgtcaccgctgggaggtcactcctatcctctgggaagtcc



 


tagccagtctccagagcaattcaagatgcagaagctgctggagctgataagagaaaagtcggaggaaatggcccagagacagctctt


 


gaaggaccaaggcctcacaaaagaacacccaaaaagagtccttcggtctcaaggcagcaccctccgggtccagcagagacctcaaa


 


attccaaggtgacacatatctcaagctgctttgggcacaagatagaccggatcggatccgtcagtcgtttgggctgtaacgcactgaagtt


 


gttgtag


 


Brain natriuretic peptide (BNP) Human (nucleic acid sequence):


(SEQ ID NO: 36)



atggatccccagacagcaccttcccgggcgctcctgctcctgctcttcttgcatctggctttcctgggaggtcgttcccacccgctgggca



 


gccccggttcagcctcggacttggaaacgtccgggttacaggagcagcgcaaccatttgcagggcaaactgtcggagctgcaggtgg


 


agcagacatccctggagcccctccaggagagcccccgtcccacaggtgtctggaagtcccgggaggtagccaccgagggcatccgt


 


gggcaccgcaaaatggtcctctacaccctgcgggcaccacgaagccccaagatggtgcaagggtctggctgctttgggaggaagatg


 


gaccggatcagctcctccagtggcctgggctgcaaagtgctgaggcggcattaa


 


Renin Mouse (nucleic acid sequence):


(SEQ ID NO: 37)



atggacagaaggaggatgcctctctgggcactcttgttgctctggagtccttgcaccttcagtctcccaacacgcaccgctacctttgaac



 


gaatcccgctcaagaaaatgccttctgtccgggaaatcctggaggagcggggagtggacatgaccaggctcagtgctgaatggggcg


 


tattcacaaagaggccttccttgaccaatcttacctcccccgtggtcctcaccaactacctgaatacccagtactacggcgagattggcat


 


cggtaccccaccccagaccttcaaagtcatctttgacacgggttcagccaacctctgggtgccctccaccaagtgcagccgcctctacct


 


tgcttgtgggattcacagcctctatgagtcctctgactcctccagctacatggagaacgggtccgacttcaccatccactacggatcagg


 


gagagtcaaaggtttcctcagccaggactcggtgactgtgggtggaatcactgtgacacagacctttggagaggtcaccgagctgccc


 


ctgatccctttcatgctggccaagtttgacggtgttctaggcatgggctttcccgctcaggccgttggcggggttacccctgtctttgacca


 


cattctctcccagggggtgctaaaggaggaagtgttctctgtctactacaacaggggttcccacctgctggggggcgaggtggtgctag


 


gaggtagcgacccgcagcattatcaaggcaattttcactatgtgagcatcagcaagactgactcctggcagatcacgatgaagggggt


 


gtctgtggggtcttccaccctgctatgtgaagaaggctgtgcggtagtggtggacactggttcatcctttatctcggctcctacgagctccc


 


tgaagttgatcatgcaagccctgggagccaaggagaagagaatagaagaatatgttgtgaactgtagccaggtgcccaccctccccga


 


catttcctttgacctgggaggcagggcctacacactcagcagtacggactacgtgctacagtatcccaacaggagagacaagctgtgc


 


acactggctctccatgccatggacatcccaccacccactgggcctgtctgggtcctgggtgccaccttcatccgcaagttctatacagag


 


tttgatcggcataacaatcgcattggattcgccttggcccgctaa


 


Renin Human (nucleic acid sequence):


(SEQ ID NO: 38)



atggatggatggagaaggatgcctcgctggggactgctgctgctgctctggggctcctgtacctttggtctcccgacagacaccaccac



 


ctttaaacggatcttcctcaagagaatgccctcaatccgagaaagcctgaaggaacgaggtgtggacatggccaggcttggtcccgagt


 


ggagccaacccatgaagaggctgacacttggcaacaccacctcctccgtgatcctcaccaactacatggacacccagtactatggcga


 


gattggcatcggcaccccaccccagaccttcaaagtcgtctttgacactggttcgtccaatgtttgggtgccctcctccaagtgcagccgt


 


ctctacactgcctgtgtgtatcacaagctcttcgatgcttcggattcctccagctacaagcacaatggaacagaactcaccctccgctattc


 


aacagggacagtcagtggctttctcagccaggacatcatcaccgtgggtggaatcacggtgacacagatgtttggagaggtcacggag


 


atgcccgccttacccttcatgctggccgagtttgatggggttgtgggcatgggcttcattgaacaggccattggcagggtcacccctatct


 


tcgacaacatcatctcccaaggggtgctaaaagaggacgtcttctctttctactacaacagagattccgagaattcccaatcgctgggag


 


gacagattgtgctgggaggcagcgacccccagcattacgaagggaatttccactatatcaacctcatcaagactggtgtctggcagattc


 


aaatgaagggggtgtctgtggggtcatccaccttgctctgtgaagacggctgcctggcattggtagacaccggtgcatcctacatctcag


 


gttctaccagctccatagagaagctcatggaggccttgggagccaagaagaggctgtttgattatgtcgtgaagtgtaacgagggccct


 


acactccccgacatctctttccacctgggaggcaaagaatacacgctcaccagcgcggactatgtatttcaggaatcctacagtagtaaa


 


aagctgtgcacactggccatccacgccatggatatcccgccacccactggacccacctgggccctgggggccaccttcatccgaaagt


 


tctacacagagtttgatcggcgtaacaaccgcattggcttcgccttggcccgctga


 


Galanin Mouse (nucleic acid sequence):


(SEQ ID NO: 39)



atggccagaggcagcgttatcctgctaggctggctcctgttggttgtgaccctgtcagccactctgggacttgggatgcctgcaaagga



 


gaagagaggttggaccctgaacagcgctggctaccttctgggcccacatgccattgacaaccacagatcatttagcgacaagcatggc


 


ctcacaggcaagagggagttacaactggaggtggaggaaaggagaccaggaagtgttgatgtgcccctgcctgagagcaacattgtc


 


cgcactataatggagtttctcagtttcttgcaccttaaagaggccggggccctcgacagcctgcctggcatccccttggccacctcctcag


 


aagacctagagaagtcctga


 


Galanin Human (nucleic acid sequence):


(SEQ ID NO: 40)



atggcccgaggcagcgccctcctgctcgcctccctcctcctcgccgcggccctttctgcctctgcggggctctggtcgccggccaagg



 


aaaaacgaggctggaccctgaacagcgcgggctacctgctgggcccacatgccgttggcaaccacaggtcattcagcgacaagaat


 


ggcctcaccagcaagcgggagctgcggcccgaagatgacatgaaaccaggaagctttgacaggtccatacctgaaaacaatatcatg


 


cgcacaatcattgagtttctgtctttcttgcatctcaaagaggccggtgccctcgaccgcctcctggatctccccgccgcagcctcctcag


 


aagacatcgagcggtcctga


 


Orexin Mouse (nucleic acid sequence):


(SEQ ID NO: 41)



atgaactttccttctacaaaggttccctgggccgccgtgacgctgctgctgctgctactgctgccgccggcgctgctgtcgcttggggtg



 


gacgcacagcctctgcccgactgctgtcgccagaagacgtgttcctgccgtctctacgaactgttgcacggagctggcaaccacgctg


 


cgggtatcctgactctgggaaagcggcggcctggacctccaggcctccagggacggctgcagcgcctccttcaggccaacggtaac


 


cacgcagctggcatcctgaccatgggccgccgcgcaggcgcagagctagagccacatccctgctctggtcgcggctgtccgaccgt


 


aactaccaccgctttagcaccccggggagggtccggagtctga


 


Orexin Human (nucleic acid sequence):


(SEQ ID NO: 42)



atgaaccttccttccacaaaggtctcctgggccgccgtgacgctactgctgctgctgctgctgtgccgcccgcgctgttgtcgtccggg



 


gcggctgcacagcccctgcccgactgctgtcgtcaaaagacttgctcttgccgcctctacgagctgctgcacggcgcgggcaatcacg


 


cggccggcatcctcacgctgggcaagcggaggtccgggcccccgggcctccagggtcggctgcagcgcctcctgcaggccagcg


 


gcaaccacgccgcgggcatcctgaccatgggccgccgcgcaggcgcagagccagcgccgcgcccctgcctcgggcgccgctgtt


 


ccgccccggccgccgcctccgtcgcgcccggaggacagtccgggatctga


 


Ghrelin-Obestatin Mouse (nucleic acid sequence):


(SEQ ID NO: 43)



atgctgtcttcaggcaccatctgcagtttgctgctactcagcatgctctggatggacatggccatggcaggctccagcttcctgagcccag



 


agcaccagaaagcccagcagagaaaggaatccaagaagccaccagctaaactgcagccacgagctctggaaggctggctccaccc


 


agaggacagaggacaagcagaagagacagaggaggagctggagatcaggttcaatgctcccttcgatgttggcatcaagctgtcag


 


gagctcagtatcagcagcatggccgggccctggggaagtttcttcaggatatcctctgggaagaggtcaaagaggcgccagctgaca


 


agtaa


 


Ghrelin-Obestatin Human (nucleic acid sequence):


(SEQ ID NO: 44)



atgccctccccagggaccgtctgcagcctcctgctcctcggcatgctctggctggacttggccatggcaggctccagcttcctgagccc



 


tgaacaccagagagtccagagaaaggagtcgaagaagccaccagccaagctgcagccccgagctctagcaggctggctccgcccg


 


gaagatggaggtcaagcagaaggggcagaggatgaactggaagtccggttcaacgccccctttgatgttggatcaagctgtcaggg


 


gttcagtaccagcagcacagccaggccctggggaagtttcttcaggacatcctctgggaagaggccaaagaggccccagccgacaa


 


gtga


 


Cholecystokinin Mouse (nucleic acid sequence):


(SEQ ID NO: 45)



atgaagagcggcgtatgtctgtgcgtggtgatggcagtcctagctgctggcgccctggcgcagccggtagtccctgcagaagctacgg



 


accccgtggagcagcgggcgcaagaggcgccccgaaggcagctgcgggctgtgctccggacggacggcgagccccgagcgcg


 


cctgggcgcactgctagcgcgatacatccagcaggtccgcaaagctccttctggccgcatgtccgttcttaagaacctgcagagcctgg


 


accccagccatagaataagtgaccgggactacatgggctggatggattttggccggcgcagtgccgaggactacgaatacccatcgta


 


g


 


Cholecystokinin Human (nucleic acid sequence):


(SEQ ID NO: 46)



atgaacagcggcgtgtgcctgtgcgtgctgatggcggtactggcggctggcgccctgacgcagccggtgcctcccgcagatcccgc



 


gggctccgggctgcagcgggcagaggaggcgccccgtaggcagctgagggtatcgcagagaacggatggcgagtcccgagcgc


 


acctgggcgccctgctggcaagatacatccagcaggcccggaaagctccttctggacgaatgtccatcgttaagaacctgcagaacct


 


ggaccccagccacaggataagtgaccgggactacatgggctggatggattttggccgtcgcagtgccgaggagtatgagtacccctc


 


ctag


 


Gastrin Mouse (nucleic acid sequence):


(SEQ ID NO: 47)



atgcctcgactgtgtgtgtacatgctggtcttagtgctggctctagctaccttctcggaagcttcttggaagccccgctcccagctacagga



 


tgcatcatctggaccagggaccaatgaggacctggaacagcgccagttcaacaagctgggctcagcctctcaccatcgaaggcagct


 


ggggccccagggtcctcaacacttcatagcagacctgtccaagaagcagaggccacgaatggaggaagaagaagaggcctacgga


 


tggatggactttggccgccgcagtgctgaggaagaccagtag


 


Gastrin Human (nucleic acid sequence):


(SEQ ID NO: 48)



atgcagcgactatgtgtgtatgtgctgatctttgcactggctctggccgccttctctgaagcttcttggaagccccgctcccagcagccag



 


atgcacccttaggtacaggggccaacagggacctggagctaccctggctggagcagcagggcccagcctctcatcatcgaaggcag


 


ctgggaccccagggtcccccacacctcgtggcagacccgtccaagaagcagggaccatggctggaggaagaagaagaagcctatg


 


gatggatggacttcggccgccgcagtgctgaggatgagaactaa


 


Protachykinin-1 (Substance P, Neurokinin A, Neuropeptide K, Neuropeptide gamma) Mouse


(nucleic acid sequence):


(SEQ ID NO: 49)



atgaaaatcctcgtggccgtggcggtcttttttctcgtttccactcaactgtttgcagaggaaatcgatccaacgatgatctaaattattggt



 


ccgactggtccgacagtgaccagatcaaggaggcaatgccggagccctttgagcatcttctgcagagaatcgcccgaagacccaagc


 


ctcagcagttctttggattaatgggcaagcgggatgctgattcctcagttgaaaaacaagtggccctgttaaaggctctttatggacatggc


 


cagatctctcacaaaaggcataaaacagattcctttgttggactaatgggcaaaagagctttaaattctgtggcttatgaaagaagcgcga


 


tgcagaactacgaaagaagacgtaaataa


 


Protachykinin-1 (Substance P, Neurokinin A, Neuropeptide K, Neuropeptide gamma) Human


(nucleic acid sequence):


(SEQ ID NO: 50)



atgaaaatcctcgtggccttggcagtcttttttcttgtctccactcagctgtttgcagaagaaataggagccaatgatgatctgaattactggt



 


ccgactggtacgacagcgaccagatcaaggaggaactgccggagccctttgagcatcttctgcagagaatcgcccggagacccaag


 


cctcagcagttctttggattaatgggcaaacgggatgctgattcctcaattgaaaaacaagtggccctgttaaaggctctttatggacatgg


 


ccagatctctcacaaaagacataaaacagattcctttgttggactaatgggcaaaagagctttaaattctgtggcttatgaaaggagtgcaa


 


tgcagaattatgaaagaagacgttaa


 


Proenkephalin-A Mouse (nucleic acid sequence):


(SEQ ID NO: 51)



atggcgcggttcctgaggctttgcacctggctgctggcgcttgggtcctgcctcctggctacagtgcaggcggaatgcagccaggact



 


gcgctaaatgcagctaccgcctggttcgcccaggcgacatcaatttcctggcgtgcacactggaatgtgaaggacagctgccttctttca


 


aaatctgggagacctgcaaggatctcctgcaggtgtccaggcccgagttcccttgggataacatcgacatgtacaaagacagcagcaa


 


acaggatgagagccacttgctagccaagaagtacggaggcttcatgaaacggtacggaggcttcatgaagaagatggacgagctatat


 


cccatggagccagaagaagaagcgaacggaggagagatccttgccaagaggtatggcggcttcatgaagaaggatgcagatgagg


 


gagacaccttggccaactcctccgatctgctgaaagagctactgggaacgggagacaaccgtgcgaaagacagccaccaacaagag


 


agcaccaacaatgacgaagacatgagcaagaggtatgggggcttcatgagaagcctcaaaagaagcccccaactggaagatgaagc


 


aaaagagctgcagaagcgctacgggggcttcatgagaagggtgggacgccccgagtggtggatggactaccagaagaggtatggg


 


ggcttcctgaagcgctttgctgagtctctgccctccgatgaagaaggcgaaaattactcgaaagaagttcctgagatagagaaaagatac


 


gggggctttatgcggttctga


 


Proenkephalin-A Human (nucleic acid sequence):


(SEQ ID NO: 52)



atggcgcggttcctgacactttgcacttggctgctgttgctcggccccgggctcctggcgaccgtgcgggccgaatgcagccaggattg



 


cgcgacgtgcagctaccgcctagtgcgcccggccgacatcaacttcctggcttgcgtaatggaatgtgaaggtaaactgccttctctga


 


aaattgggaaacctgcaaggagctcctgcagctgtccaaaccagagcttcctcaagatggcaccagcaccctcagagaaaatagcaa


 


accggaagaaagccatttgctagccaaaaggtatgggggcttcatgaaaaggtatggaggcttcatgaagaaaatggatgagctttatc


 


ccatggagccagaagaagaggccaatggaagtgagatcctcgccaagcggtatgggggcttcatgaagaaggatgcagaggagga


 


cgactcgctggccaattcctcagacctgctaaaagagcttctggaaacaggggacaaccgagagcgtagccaccaccaggatggca


 


gtgataatgaggaagaagtgagcaagagatatgggggcttcatgagaggcttaaagagaagcccccaactggaagatgaagccaaa


 


gagctgcagaagcgatatgggggcttcatgagaagagtaggtcgcccagagtggtggatggactaccagaaacggtatggaggtttc


 


ctgaagcgctttgccgaggctctgccctccgacgaagaaggcgaaagttactccaaagaagttcctgaaatggaaaaaagatacggag


 


gatttatgagattttaa


 


Proenkephalin-B Mouse (nucleic acid sequence):


(SEQ ID NO: 53)



atggcgtggtccaggctgatgctggcagcttgcctcctcgtgatgccctctaatgttatggcggactgcctgtccctgtgctccctgtgtgc



 


agtgaggattcaggatgggccccgtcccatcaaccccctgatttgctccctggagtgccaggacctggtgccgccctcagaggagtgg


 


gagacatgccgggcttctcatcttttctcaccctgacggtctctgggctccgtggcaaggatgacttggaagatgaggttgctttggaag


 


aaggctacagtgcactagccaagctcttggaacccgtcctgaaggagctggagaaaagccgactccttaccagcgtcccagaggaaa


 


agttcaggggtctctccagcagctttggcaacggaaaagaatctgagctggcgggtgctgaccggatgaatgatgaagccgcacagg


 


cgggcacgctccattttaatgaggaggacttgagaaaacaggccaaacgctatggcggctttttgcgcaaataccccaagaggagttcc


 


gagatggcccgggatgaggacgggggccaggatggggatcaggtagggcatgaggacctgtacaaacgctatgggggcttcctgc


 


ggcgcattcgccccaagctgaagtgggacaaccagaagcgctatggtggtttcctgcggcgtcagttcaaggtggtgacgcggtccca


 


ggagaaccccaatacctattctgaagatttagatgtttga


 


Proenkephalin-B Human (nucleic acid sequence):


(SEQ ID NO: 54)



atggcctggcaggggctggtcctggctgcctgcctcctcatgttcccctccaccacagcggactgcctgtcgcggtgctccttgtgtgct



 


gtaaagacccaggatggtcccaaacctatcaatcccctgatttgctccctgcaatgccaggctgccctgctgccctctgaggaatggga


 


gagatgccagagctttctgtcttttttcaccccctccacccttgggctcaatgacaaggaggacttggggagcaagtcggttggggaagg


 


gccctacagtgagctggccaagctctctgggtcattcctgaaggagctggagaaaagcaagtttctcccaagtatctcaacaaaggaga


 


acactctgagcaagagcctggaggagaagctcaggggtctctctgacgggtttagggagggagcagagtctgagctgatgagggatg


 


cccagctgaacgatggtgccatggagactggcacactctatctcgctgaggaggaccccaaggagcaggtcaaacgctatgggggct


 


ttttgcgcaaataccccaagaggagctcagaggtggctggggagggggacggggatagcatgggccatgaggacctgtacaaacgc


 


tatgggggcttcttgcggcgcattcgtcccaagctcaagtgggacaaccagaagcgctatggcggttttctccggcgccagttcaaggt


 


ggtgactcggtctcaggaagatccgaatgcttactctggagagctttttgatgcataa


 


Insulin-like growth hormone 1 (IGF-1) Mouse (nucleic acid sequence):


(SEQ ID NO: 55)



atggggaaaatcagcagccttccaactcaattatttaagatctgcctctgtgacttcttgaagataaagatacacatcatgtcgtcttcacac



 


ctcttctacctggcgctctgcttgctcaccttcaccagctccaccacagctggaccagagaccctttgcggggctgagctggtggatgct


 


cttcagttcgtgtgtggaccgaggggcttttacttcaacaagcccacaggctatggctccagcattcggagggcacctcagacaggcatt


 


gtggatgagtgttgcttccggagctgtgatctgaggagactggagatgtactgtgccccactgaagcctacaaaagcagcccgctctat


 


ccgtgcccagcgccacactgacatgcccaagactcagaagtccccgtccctatcgacaaacaagaaaacgaagctgcaaaggagaa


 


ggaaaggaagtacatttgaagaacacaagtag


 


Insulin-like growth hormone 1 (IGF-1) Human (nucleic acid sequence):


(SEQ ID NO: 56)



atgggaaaaatcagcagtcttccaacccaattatttaagtgctgcttttgtgatttcttgaaggtgaagatgcacaccatgtcctcctcgcatc



 


tcttctacctggcgctgtgcctgctcaccttcaccagctctgccacggctggaccggagacgctgctgcggggctgagctggtggatgct


 


cttcagttcgtgtgtggagacaggggcttttatttcaacaagcccacagggtatggctccagcagtcggagggcgcctcagacaggcat


 


cgtggatgagtgctgcttccggagctgtgatctaaggaggctggagatgtattgcgcacccctcaagcctgccaagtcagctcgctctgt


 


ccgtgcccagcgccacaccgacatgcccaagacccagaagtatcagcccccatctaccaacaagaacacgaagtctcagagaagga


 


aaggaagtacatttgaagaacgcaagtag


 


Insulin-like growth hormone 2 (IGF-2) Mouse (nucleic acid sequense):


(SEQ ID NO: 57)



atgggcggcagcgtcgccggcttccaggtaccaatggggatcccagtggggaagtcgatgttggtgcttctcatctctttggccttcgcc



 


ttgtgctgcatcgctgcttacggccccggagagactctgtgcggaggggagcttgttgacacgcttcagtttgtctgttcggaccgcggc


 


ttctacttcagcaggccttcaagccgtgccaaccgtcgcagccgtggcatcgtggaagagtgctgcttccgcagctgcgacctggccct


 


cctggagacatactgtgccacccccgccaagtccgagagggacgtgtctacctctcaggccgtacttccggacgacttccccagatac


 


cccgtgggcaagttcttccaatatgacacctggagacagtccgcgggacgcctgcgcagaggcctgcctgccctcctgcgtgcccgc


 


cggggtcgcatgcttgccaaagagctcaaagagttcagagaggccaaacgtcatcgtcccctgatcgtgttaccacccaaagaccccg


 


cccacgggggagcctcttcggagatgtccagcaaccatcagtga


 


Insulin-like growth hormone 2 (IGF-2) Human (nucleic acid sequence):


(SEQ ID NO: 58)



atgggaatcccaatggggaagtcgatgctggtgcttctcaccttcttggccttcgcctcgtgctgcattgctgcttaccgccccagtgaga



 


ccctgtgcggcggggagctggtggacaccctccagttcgtctgtggggaccgcggcttctacttcagcaggcccgcaagccgtgtga


 


gccgtcgcagccgtggcatcgttgaggagtgctgtttccgcagctgtgacctggccctcctggagacgtactgtgctacccccgccaag


 


tccgagagggacgtgtcgacccctccgaccgtgcttccggacaacttccccagataccccgtgggcaagttcttccaatatgacacctg


 


gaagcagtccacccagcgcctgcgcaggggcctgcctgccctcctgcgtgcccgccggggtcacgtgctcgccaaggagctcgag


 


gcgttcagggaggccaaacgtcaccgtcccctgattgctctacccacccaagaccccgcccacgggggcgcccccccagagatggc


 


cagcaatcggaagtga


 


Parathyroid hormone (PTH) Mouse (nucleic acid sequence):


(SEQ ID NO: 59)



atgatgtctgcaaacaccgtggctaaagtgatgatcatcatgctggcagtctgtcttcttacccaaacggatgggaaacccgtgaggaag



 


agagctgtcagtgaaatacagcttatgcacaacctgggcaaacacctggcctccatggagaggatgcaatggctgagaaggaagctg


 


caagatatgcacaattttgttagtcttggagtccaaatggctgccagagatggcagtcaccagaagcccaccaagaaggaggaaaatgt


 


ccttgttgatggcaatccaaaaagtcttggtgagggagacaaagctgatgtggatgtattagttaaatcaaaatctcagtaa


 


Parathyroid hormone (PTH) Human (nucleic acid sequence):


(SEQ ID NO: 60)



atgatacctgcaaaagacatggctaaagttatgattgtcatgttggcaatttgttttcttacaaaatcggatgggaaatctgttaagaagagat



 


ctgtgagtgaaatacagcttatgcataacctgggaaaacatctgaactcgatggagagagtagaatggctgcgtaagaagctgcaggat


 


gtgcacaattttgttgcccttggagctcctctagctcccagagatgctggttcccagaggccccgaaaaaaggaagacaatgtcttggttg


 


agagccatgaaaaaagtcttggagaggcagacaaagctgatgtgaatgtattaactaaagctaaatcccagtga


 


Parathyroid hormone-related protein (PTHrP) Mouse (nucleic acid sequence):


(SEQ ID NO: 61)



atgctgcggaggctggttcagcagtggagtgtcctggtattcctgctcagctactccgtgccctcccgcgggcgttcggtggaggggct



 


tggccgcaggctcaaacgcgctgtgtctgaacatcagctactgcatgacaagggcaagtccatccaagacttgcgccgccgtttcttcct


 


ccaccatctgatcgcggagatccacacagccgaaatcagagctacctcggaggtgtcccccaactccaaacctgctcccaacaccaaa


 


aaccaccccgtgcggtttgggtcagacgatgagggcagatacctaactcaggaaaccaacaaggtggagacgtacaaagaacagcc


 


actcaagacacccgggaagaagaagaaaggcaagcctgggaaacgcagagaacaggagaaaaagaagcgaaggactcggtctg


 


cctggccaagcacagctgcgagtggcctgcttgaggaccccctgccccacacctccaggccctcgctggagcccagcttaaggacg


 


cattga


 


Parathyroid hormone-related protein (PTHrP) Human (nucleic acid sequence):


(SEQ ID NO: 62)



atgcagcggagactggttcagcagtggagcgtcgcggtgttcctgctgagctacgcggtgccctcctgcgggcgctcggtggagggt



 


ctcagccgccgcctcaaaagagctgtgtctgaacatcagctcctccatgacaaggggaagtccatccaagatttacggcgacgattcttc


 


cttcaccatctgatcgcagaaatccacacagctgaaatcagagctacctcggaggtgtcccctaactccaagccctctcccaacacaaa


 


gaaccaccccgtccgatttgggtctgatgatgagggcagatacctaactcaggaaactaacaaggtggagacgtacaaagagcagcc


 


gctcaagacacctgggaagaaaaagaaaggcaagcccgggaaacgcaaggagcaggaaaagaaaaaacggcgaactcgctctgc


 


ctggttagactctggagtgactgggagtgggctagaaggggaccacctgtctgacacctccacaacgtcgctggagctcgattcacgg


 


aggcattga


 


Osteocalcin Mouse (nucleic acid sequence):


(SEQ ID NO: 63)



atgaggaccatctttctgctcactctgctgaccctggctgcgctctgtctctctgacctcacagatgccaagcccagcggccctgagtctg



 


acaaagccttcatgtccaagcaggagggcaataaggtagtgaacagactccggcgctaccttggagcctcagtccccagcccagatc


 


ccctggagcccacccgggagcagtgtgagcttaaccctgcttgtgacgagctatcagaccagtatggcttgaagaccgcctacaaacg


 


catctatggtatcactatttag


 


Osteocalcin Human (nucleic acid sequence):


(SEQ ID NO: 64)



atgagagccctcacactcctcgccctattggccctggccgcactttgcatcgctggccaggcaggtgcgaagcccagcggtgcagagt



 


ccagcaaaggtgcagcctttgtgtccaagcaggagggcagcgaggtagtgaagagacccaggcgctacctgtatcaatggctggga


 


gccccagtcccctacccggatcccctggagcccaggagggaggtgtgtgagctcaatccggactgtgacgagttggctgaccacatc


 


ggctttcaggaggcctatcggcgcttctacggcccggtctag


 


Urocortin-3 Mouse (nucleic acid sequence):


(SEQ ID NO: 65)



atgctgatgcccacctacttcctgctgccacttctgctgctcctaggaggtccaaggacaagcctctcccacaagttctacaacactggac



 


cagtcttcagctgcctcaacacagccctatctgaggtcaagaagaacaagctggaagatgtgcccttgctgagcaagaagagctttggc


 


cacctgcccacacaagacccctcaggggaagaagatgacaaccaaacgcacctccagatcaaaagaactttctcaggtgccgcgggt


 


gggaatggagctgggagcacccggtacagataccaatcccaggcacagcacaaggggaagctgtacccagacaagcccaaaagc


 


gaccggggcaccaagttcaccctttcccttgatgttcccactaacatcatgaacatcctcttcaacatcgacaaggccaagaatttgcgag


 


ccaaggcagctgccaatgctcagctcatggcacagattgggaagaagaagtaa


 


Urocortin-3 Human (nucleic acid sequence):


(SEQ ID NO: 66)



atgctgatgccggtccacttcctgctgctcctgctgctgctcctggggggccccaggacaggcctcccccacaagttctacaaagccaa



 


gcccatcttcagctgcctcaacaccgccctgtctgaggctgagaagggccagtgggaggatgcatccctgctgagcaagaggagcttc


 


cactacctgcgcagcagagacgcctcttcgggagaggaggaggagggcaaagagaaaaagactttccccatctctggggccaggg


 


gtggagccagaggcacccggtacagatacgtgtcccaagcacagcccaggggaaagccacgccaggacacggccaagagtcccc


 


accgcaccaagttcaccctgtccctcgacgtccccaccaacatcatgaacctcctcttcaacatcgccaaggccaagaacctgcgtgcc


 


caggcggccgccaatgcccacctgatggcgcaaattgggaggaagaagtag


 


Urocortin-2 Mouse (nucleic acid sequence):


(SEQ ID NO: 67)



atgatgaccaggtgggcactggtggtgttcgtggtcctgatgttggataggatcctatttgtcccaggaactcctatccccaccttccagct



 


cctccctcagaactctctggagacaactcctagctctgtgacctcagagagctcctcaggtaccaccacaggaccctcagcttcctgga


 


gcaactctaaagccagcccttacctagacacccgtgtcatactctccctggatgttcccattggcctcctacggatcttactggaacaggc


 


tcgttacaaggctgccaggaatcaggctgccactaatgctcaaatactagcccatgttggccgccgctga


 


Urocortin-2 Human (nucleic acid sequence):


(SEQ ID NO: 68)



atgaccaggtgtgctctgctgttgctgatggtcctgatgttgggcagagtcctggttgtcccagtgacccctatcccaaccttccagctccg



 


ccctcagaattctccccagaccactccccgacctgcggcctcagagagcccctcagctgctcccacatggccgtgggctgcccagag


 


ccactgcagccccacccgccaccctggctcgcgcattgtcctatcgctggatgtccccatcggcctcttgcagatcttactggagcaagc


 


ccgggccagggctgccagggagcaggccaccaccaacgcccgcatcctggcccgtgtcggccactgctga


 


Urocortin-1 Mouse (nucleic acid sequence):


(SEQ ID NO: 69)



atgatacagaggggacgcgctacgctcctggtggcgttgctgctcttggcacagcttcgcccggagagcagccagtggagcccagcg



 


gctgcggcggcaactggggtccaggatccgaatctgcgatggagccctggagtgcggaatcagggcggcggcgtccgcgcgctcc


 


tcttgctgttagcggagcgcttcccgcgccgcgcaggatctgagcctgcgggcgagcggcagcgacgggacgaccctccactgtcc


 


atcgacctcaccttccacctgctgcggaccctgctggagctagctcggacacagagccagcgcgagcgcgcagagcagaaccgcat


 


catattcgattcggtgggcaagtga


 


Urocortin-1 Human (nucleic acid sequence):


(SEQ ID NO: 70)



atgaggcaggcgggacgcgcagcgctgctggccgcgctgctgctcctggtacagctgtgccctgggagcagccagaggagccccg



 


aggcggccggggtccaggacccgagtctgcgctggagccccggggcacggaaccagggtggcggggcccgcgcgctcctcttgc


 


tgctggcggagcgcttcccgcgccgcgcggggcccggccgattgggactcgggacggcaggcgagcggccgcggcgggacaac


 


ccttctctgtccattgacctcacctttcacctgctgcggaccctgctggagctggcgcggacgcagagccagcgggagcgcgccgagc


 


agaaccgcatcatattcgactcggtgggcaagtga


 


FGF23 Mouse (nucleic acid sequence):


(SEQ ID NO: 71)



Atgctagggacctgccttagactcctggtgggcgtgctctgcactgtctgcagcttgggcactgctagagcctatccggacacttcccc



 


attgcttggctccaactggggaagcctgacccacctgtacacggctacagccaggaccagctatcacctacagatccatagggatggtc


 


atgtagatggcaccccccatcagaccatctacagtgccctgatgattacatcagaggacgccggctctgtggtgataacaggagccatg


 


actcgaaggttcctttgtatggatctccacggcaacatttttggatcgcttcacttcagcccagagaattgcaagttccgccagtggacgct


 


ggagaatggctatgacgtctacttgtcgcagaagcatcactacctggtgagcctgggccgcgccaagcgcatcttccagccgggcacc


 


aacccgccgcccttctcccagttcctggctcgcaggaacgaggtcccgctgctgcatttctacactgttcgcccacggcgccacacgcg


 


cagcgccgaggacccaccggagcgcgacccactgaacgtgctcaagccgcggccccgcgccacgcctgtgcctgtatcctgctctc


 


gcgagctgccgagcgcagaggaaggtggccccgcagccagcgatcctctgggggtgctgcgcagaggccgtggagatgctcgcg


 


ggggcgcgggaggcgcggataggtgtcgcccctttcccaggttcgtctag


 


FGF23 Human (nucleic acid sequence):


(SEQ ID NO: 72)



Atgagggggcccgcctcaggctctgggtctgtgccttgtgcagcgtctgcagcatgagcgtcctcagagcctatcccaatgcctcccc



 


actgctcggctccagctggggtggcctgatccacctgtacacagccacagccaggaacagctaccacctgcagatccacaagaatgg


 


ccatgtggatggcgcaccccatcagaccatctacagtgccctgatgatcagatcagaggatgctggctttgtggtgattacaggtgtgat


 


gagcagaagatacctctgcatggatttcagaggcaacatttttggatcacactatttcgacccggagaactgcaggttccaacaccagac


 


gctggaaaacgggtacgacgtctaccactctcctcagtatcacttcctggtcagtctgggccgggcgaagagagccttcctgccaggca


 


tgaacccacccccgtactcccagttcctgtcccggaggaacgagatccccctaattcacttcaacaccccataccacggcggcacac


 


ccggagcgccgaggacgactcggagcgggaccccctgaacgtgctgaagccccgggcccggatgaccccggccccggcctcctg


 


ttcacaggagctcccgagcgccgaggacaacagcccgatggccagtgacccattaggggtggtcaggggcggtcgagtgaacacg


 


cacgctgggggaacgggcccggaaggctgccgccccttcgccaagttcatctag


 


IL1B Mouse (nucleic acid sequence):


(SEQ ID NO: 73)



Atggcaactgttcctgaactcaactgtgaaatgccaccttttgacagtgatgagaatgacctgttctttgaagttgacggaccccaaaaga



 


tgaagggctgcttccaaacctttgacctgggctgtcctgatgagagcatccagcttcaaatctcgcagcagcacatcaacaagagcttca


 


ggcaggcagtatcactcattgtggctgtggagaagctgtggcagctacctgtgctttcccgtggaccttccaggatgaggacatgagca


 


ccttcttttccttcatctttgaagaagagcccatcctctgtgactcatgggatgatgatgataacctgctggtgtgtgacgttcccattagaca


 


actgcactacaggctccgagatgaacaacaaaaaagcctcgtgctgtcggacccatatgagctgaaagctctccacctcaatggacag


 


aatatcaaccaacaagtgatattctccatgagctttgtacaaggagaaccaagcaacgacaaaatacctgtggccttgggcctcaaagg


 


aaagaatctatacctgtcctgtgtaatgaaagacggcacacccaccctgcagctggagagtgtggatcccaagcaatacccaaagaag


 


aagatggaaaaacggtttgtcttcaacaagatagaagtcaagagcaagtggagtttgagtctgcagagttccccaactggtacatcagc


 


acctcacaagcagagcacaagcctgtcttcctgggaaacaacagtggtcaggacataattgacttcaccatggaatccgtgtcttcctaa


 


IL1B Human (nucleic acid sequence):


(SEQ ID NO: 74)



Atggcagaagtacctgagctcgccagtgaaatgatggcttattacagtggcaatgaggatgacttgttctttgaagctgatggccctaaa



 


cagatgaagtgctccttccaggacctggacctctgccctctggatggcggcatccagctacgaatctccgaccaccactacagcaagg


 


gcttcaggcaggccgcgtcagttgttgtggccatggacaagctgaggaagatgctggttccctgcccacagaccttccaggagaatga


 


cctgagcaccttctttcccttcatctttgaagaagaacctatcttcttcgacacatgggataacgaggcttatgtgcacgatgcacctgtacg


 


atcactgaactgcacgctccgggactcacagcaaaaaagcttggtgatgtctggtccatatgaactgaaagctctccacctccagggac


 


aggatatggagcaacaagtggtgttctccatgtcctttgtacaaggagaagaaagtaatgacaaaatacctgtggccttgggcctcaagg


 


aaaagaatctgtacctgtcctgcgtgttgaaagatgataagcccactctacagctggagagtgtagatcccaaaaattacccaaagaaga


 


agatggaaaagcgatttgtcttcaacaagatagaaatcaataacaagctggaatttgagtctgcccagttccccaactggtacatcagcac


 


ctctcaagcagaaaacatgcccgtcttcctgggagggaccaaaggcggccaggatataactgacttcaccatgcaatttgtgtcttccta


 


a


 


TNFA Mouse (nucleic acid sequence):


(SEQ ID NO: 75)



Atgagcacagaaagcatgatccgcgacgtggaactggcagaagaggcactcccccaaaagatggggggcttccagaactccaggc



 


ggtgcctatgtctcagcctcttctcattcctgcttgtggcaggggccaccacgctcttctgctactgaacttcggggtgatcggtccccaa


 


agggatgagaagttcccaaatggcctccctctcatcagttctatggcccagaccctcacactcagatcatcttctcaaaattcgagtgaca


 


agcctgtagcccacgtcgtagcaaaccaccaagtggaggagcagctggagtggctgagccagcgcgccaacgccctcctggccaa


 


cggcatggatctcaaagacaaccaactagtggtgccagccgatgggttgtaccttgtctactcccaggttctcttcaagggacaaggctg


 


ccccgactacgtgctcctcacccacaccgtcagccgatttgctatctcataccaggagaaagtcaacctcctctctgccgtcaagagccc


 


ctgccccaaggacacccctgagggggctgagctcaaaccctggtatgagcccatatacctgggaggagtcttccagctggagaaggg


 


ggaccaactcagcgctgaggtcaatctgcccaagtacttagactttgcggagtccgggcaggtctactttggagtcattgctctgtga


 


TNFA Human (nucleic acid sequence):


(SEQ ID NO: 76)



Atgagcactgaaagcatgatccgggacgtggagctggccgaggaggcgctccccaagaagacaggggggccccagggctccag



 


gcggtgcttgttcctcagcctcttctccttcctgatcgtggcaggcgccaccacgctcttctgcctgctgcactttggagtgatcggccccc


 


agagggaagagttccccagggacctctctctaatcagccctctggcccaggcagtcagatcatcttctcgaaccccgagtgacaagcct


 


gtagcccatgttgtagcaaaccctcaagctgaggggcagctccagtggctgaaccgccgggcaatgccctcctggccatggcgtg


 


gagctgagagataaccagctggtggtgccatcagagggcctgtacctcatctactcccaggtcctcttcaagggccaaggctgcccctc


 


cacccatgtgctcctcacccacaccatcagccgcatcgccgtctcctaccagaccaaggtcaacctcctctctgccatcaagagcccct


 


gccagagggagaccccagagggggctgagccaagccctggtatgagcccatctatctgggaggggtcttccagctggagaagggt


 


gaccgactcagcgctgagatcaatcggcccgactatctcgactttgccgagtctgggcaggtctactttgggatcattgccctgtga


 


IFNG Mouse (nucleic acid sequence):


(SEQ ID NO: 77)



Atgaacgctacacactgcatcttggctttgcagctcttcctcatggctgtttctggctgttactgccacggcacagtcattgaaagcctaga



 


aagtctgaataactattttaactcaagtggcatagatgtggaagaaaagagtctcttcttggatatctggaggaactggcaaaaggatggt


 


gacatgaaaatcctgcagagccagattatctctttctacctcagactctttgaagtcttgaaagacaatcaggccatcagcaacaacataa


 


gcgtcattgaatcacacctgattactaccttcttcagcaacagcaaggcgaaaaaggatgcattcatgagtattgccaagtttgaggtcaa


 


caacccacaggtccagcgccaagcattcaatgagctcatccgagtggtccaccagctgttgccggaatccagcctcaggaagcggaa


 


aaggagtcgctgctga


 


IFNG Human (nucleic acid sequence):


(SEQ ID NO: 78)



Atgaaatatacaagttatatcttggcttttcagctctgcatcgttttgggttctcttggctgttactgccaggacccatatgtaaaagaagcag



 


aaaaccttaagaaatattttaatgcaggtcattcagatgtagcggataatggaactcttttcttaggcattttgaagaattggaaagaggaga


 


gtgacagaaaaataatgcagagccaaattgtctccttttacttcaaactttttaaaaactttaaagatgaccagagcatccaaaagagtgtg


 


gagaccatcaaggaagacatgaatgtcaagtttttcaatagcaacaaaaagaaacgagatgacttcgaaaagctgactaattattcggta


 


actgacttgaatgtccaacgcaaagcaatacatgaactcatccaagtgatggctgaactgtcgccagcagctaaaacagggaagcgaa


 


aaaggagtcagatgctgtttcgaggtcgaagagcatcccagtaa


 


Sortilin Mouse (nucleic acid sequence):


(SEQ ID NO: 79)



Atggagcggccccggggagctgcggacggccttttgcgctggcccctcggcctcctcctgctccttcaactgctgcctcctgccgccg



 


tcggccaggaccggctggacgcgccgccgccgcccgcgcctcctctgctgcgctgggccggtccggtcggggtgagctgggggct


 


gcgcgccgccgcgcccgggggccccgtcccccgcgctggccgttggcgccgcggcgcgcccgccgaggaccaagactgcggcc


 


gcctcccggacttcatcgccaagctgaccaacaatacgcaccagcatgtctttgatgacctcagtggctcagtgtccttgtcctgggttgg


 


agacagcactggggttattctcgtcctgaccactttccaagtgcctctggtaattgtgagctttggacagtccaagttgtatcgaagtgagg


 


attatggaaagaactttaaggatattacaaatctcatcaataacaccttcattcggacggaatttggcatggctattggtcctgagaactctg


 


gaaaggtgatactaacagcggaggtgtccgggggaagccgaggcggaagagtgttcaggtcatcagactttgccaagaactttgtgc


 


aaacagatctcccctttcatcctctgacgcagatgatgtacagccctcagaattctgattacctgttagctctcagcaccgaaaatggcctg


 


tgggtgtccaagaattttggggaaaaatgggaagaaatccacaaagcagtatgtttggccaaatggggaccaaacaacatcatcttcttt


 


accacccatgtgaatggctcctgcaaagctgatcttggtgccctggaattatggagaacatccgacttgggaaaaaccttcaaaaccatt


 


ggtgtgaaaatctactcctttggtcttgggggccgtttcctttttgcctctgtgatggctgataaggacacaacaagaaggatccatgtgtca


 


acagaccagggggacacatggagcatggcacaacttccttctgtgggacaggaacagttctactccatcctggcagccaatgaggaca


 


tggtcttcatgcatgtagatgaacctggagataccgggtttggcaccatctttacctctgatgatcgaggcattgtctactccaagtctctgg


 


acagacatctctataccaccacaggcggggagacggactttaccaacgtgacttccctccgtggggtctatataacaagcacgctctca


 


gaagataactctattcagagcatgatcacttttgaccagggaggacggtgggagcacctgcggaagccggagaacagcaagtgcgac


 


gctaccgcaaagaacaagaacgagtgcagccttcatatccatgcttcttatagcatctcccagaagctaaacgttccaatggccccacttt


 


ccgagcccaatgctgtgggcatagtcatcgctcacggtagtgtgggagatgccatctcggtgatggtcccagatgtgtacatctcagatg


 


atgggggttactcctgggcgaagatgctagaaggaccacattactataccatcctggactctggaggcatcattgtggccattgagcaca


 


gcaaccgtcctatcaatgtgattaagttctccacagatgaaggccagtgctggcagagctatgtgttcacacaggagcccatctacttcac


 


tgggcttgcttccgagcctggagccaggtccatgaacatcagcatctggggattcacagagtctttcattacccgccagtgggtctcctac


 


acagtcgatttcaaagacatccttgagcggaattgtgaagaggatgactataccacgtggctggcacactccacagaccctggagatta


 


caaagacggctgcattttgggctataaagaacagttcctacggctacggaagtcatccgtctgtcagaatggtcgagactatgttgtggc


 


caagcagccatccgtctgtccgtgttccctggaggacttcctctgtgactttggctacttccgtccggagaacgcctcagagtgcgtgga


 


gcagcctgaactgaaggggcatgagttagagttctgtctgtacggcaaggaggagcacctgacaacaaatgggtaccggaaaatccc


 


aggagacaaatgccaaggtgggatgaatcccgccagagaagtaaaagacttgaaaaagaaatgcacaagcaacttcttgaaccccac


 


aaagcaggactcccgcccacagggacacagcttgtcccagaatccagctccgcctcctcttggatacactgaaaacacacacttcctat


 


ctcctacccagaagcagaattccaagtcaaattctgtccctattatcctggccatcgtgggactgatgcttgtcacagtcgtagcaggagt


 


cctcattgtgaagaaatatgtctgtggcggaaggttcctggtgcaccggtactcggtgctacagcagcacgcagaggctgacggcgta


 


gaggctttggattcaacctcccacgctaaaagcggatatcacgacgactcagatgaggacctcctggaatag


 


Sortilin Human (nucleic acid sequence):


(SEQ ID NO: 80)



Atggagcggccctggggagctgcggacggcctctcgcgctggccccatggcctcggcctcctcctcctcctgcagctgctgccgcc



 


gtcgaccctcagccaggaccggctggacgcgccgccgccgcccgctgcgccgctgccgcgctggtctggccccatcggggtgagc


 


tgggggctgcgggcggccgcagccgggggcgcgtttccccgcggcggccgttggcgtcgcagcgcgccgggcgaggacgagga


 


gtgcggccgggtccgggacttcgtcgccaagctggccaacaacacgcaccagcatgtgtttgatgatctcagaggctcagtatccttgt


 


cctgggttggagatagcactggggtcattctagtcttgactaccttccatgtaccactggtaattatgacttttggacagtccaagctatatc


 


gaagtgaggattatgggaagaactttaaggatattacagatctcatcaataacacctttattcggactgaatttggcatggctattggtcctg


 


agaactctggaaaggtggtgttaacagcagaggtgtctggaggaagtcgtggaggaagaatctttagatcatcagattttgcgaagaatt


 


ttgtgcaaacagatctcccttttcatcctctcactcagatgatgtatagccctcagaattctgattatcttttagctctcagcactgaaaatggc


 


ctgtgggtgtccaagaattttgggggaaaatgggaagaaatccacaaagcagtatgtttggccaaatggggatcagacaacaccatctt


 


ctttacaacctatgcaaatggctcctgcaaagctgaccttggggctctggaattatggagaacttcagacttgggaaaaagcttcaaaact


 


attggtgtgaaaatctactcatttggtcttgggggacgtttcctttttgcctctgtgatggctgataaggatacaacaagaaggatccacgttt


 


caacagatcaaggggacacatggagcatggcccagctcccctccgtgggacaggaacagttctattctattctggcagcaaatgatga


 


catggtattcatgcatgtagatgaacctggagacactgggtttggcacaatctttacctcagatgatcgaggcattgtctattccaagtcttt


 


ggaccgacatctctacactaccacaggcggagagacggactttaccaacgtgacctccctccgcggcgtctacataacaagcgtgctc


 


tccgaagataattctatccagaccatgatcacttttgaccaaggaggaaggtggacgcacctgaggaagcctgaaaacagtgaatgtga


 


tgctacagcaaaaaacaagaatgagtgcagccttcatattcatgcttcctacagcatctcccagaaactgaatgttccaatggccccactc


 


tcagagccgaatgccgtaggcattgtcattgctcatggtagcgtgggggatgccatctcagtgatggttccagatgtgtacatctcagatg


 


atgggggttactcctggacaaagatgctggaaggaccccactattacaccatcctggattctggaggcatcattgtggccattgagcaca


 


gcagccgtcctatcaatgtgattaagttctccacagacgaaggtcaatgctggcaaacctacacgttcaccagggaccccatctatttcac


 


tggcctagcttcagaacctggagctaggtccatgaatatcagcatttggggcttcacagaatctttcctgaccagccagtgggtctcctac


 


accattgattttaaagatatccttgaaaggaactgtgaagagaaggactataccatatggctggcacactccacagaccctgaagattatg


 


aagatggctgcattttgggctacaaagaacagtttctgcggctacgcaagtcatccgtgtgtcagaatggtcgagactatgttgtgaccaa


 


gcagccctccatctgcctctgttccctggaggactttctctgtgattttggctactaccgtccagaaaatgactccaagtgtgtggaacagc


 


cagaactgaagggccacgacctggagttttgtctgtacggaagagaagaacacctaacaacaaatgggtaccggaaaattccagggg


 


acaaatgccagggtggggtaaatccagttcgagaagtaaaagacttgaaaaagaaatgcacaagcaactttttgagtccggaaaaaca


 


gaattccaagtcaaattctgttccaattatcctggccatcgtgggattgatgctggtcacagtcgtagcaggagtgctcattgtgaagaaat


 


atgtctgtgggggaaggttcctggtgcatcgatactctgtgctgcagcagcatgcagaggccaatggtgtggatggtgtggatgctttgg


 


acacagcctcccacactaataaaagtggttatcatgatgactcagatgaggacctcttggaatag


 


Neuropeptide W Mouse (nucleic acid sequence):


(SEQ ID NO: 81)



Ctggcgtctaacagagaagtgcggggccctgggcccgggactcccaggaaccggcccctgctgcccctgctgctgcactgctcttg



 


ctaccgctgcccgccagcgcctggtataagcacgtggcgagtccccgctatcacacagtgggtcgtgcctccgggctgctcatgggg


 


ctgcgccgctcgccctaccagtggcgccgtgccctgggcggggctgctggacccctctcccggctcccaggaccggtcgcccgcgg


 


cgctctcctgcttccttcctcagggcaggagctgtgggaggtacgaagcaggagctcacctgcagggcttcccgtccatgcaccctgg


 


agtccgcgggacctggagggagtccgccaaccggagcagtcgctaagccttcactcctggatctcagaggagcccgctgctagagc


 


cttcggagagacgcttcgtgcccagccatggttcctgcagcaagtcatctttgccgatcctgtcaggcccaagaaccgatggcgccccc


 


atgcttga


 


Neuropeptide W Human (nucleic acid sequence):


(SEQ ID NO: 82)



Ctggcgtggcgcccaggggagcggggggctcccgcgagccggccgcggctggcactgctgctgcttctgctcctgctgccgctgc



 


cctccggcgcgtggtacaagcacgtggcgagtccccgctaccacacggtgggccgcgccgctggcctgctcatggggctgcgtcgc


 


tcaccctatctgtggcgccgcgcgctgcgcgcggccgccgggcccctggccagggacaccctctcccccgaacccgcagcccgcg


 


aggctcctctcctgctgccctcgtgggttcaggagctgtgggagacgcgacgcaggagctcccaggcagggatccccgtccgtgcgc


 


cccggagcccgcgcgccccagagcctgcgctggaaccggagtccctggacttcagcggagctggccagagacttcggagagacgt


 


ctcccgcccagcggtggaccccgcagcaaaccgccttggcctgccctgcctggcccccggaccgttctga


 


CART Mouse (nucleic acid sequence):


(SEQ ID NO: 83)



Atggagagctcccgcctgcggctgctacccctcctgggcgccgccctgctgctactgctacctttgctgggtgcccgtgcccaggagg



 


acgccgagctgcagccccgagccctggacatctactctgccgtggatgatgcgtcccacgagaaggagctgatcgaagcgttgcaag


 


aagtcctgaagaagctcaagagtaaacgcattccgatctacgagaagaagtacggccaagtccccatgtgtgacgctggagagcagt


 


gcgcagtgaggaaaggggccaggatcgggaagctgtgtgactgtccccgaggaacttcctgcaattctttcctcttgaagtgcttgtga


 


CART Human (nucleic acid sequence):


(SEQ ID NO: 84)



Atggagagctcccgcgtgaggctgctgcccctcctgggcgccgccctgctgctgatgctacctctgttgggtacccgtgcccaggag



 


gacgccgagctccagccccgagccctggacatctactctgccgtggatgatgcctcccacgagaaggagctgatcgaagcgctgcaa


 


gaagtcttgaagaagctcaagagtaaacgtgttcccatctatgagaagaagtatggccaagtccccatgtgtgacgccggtgagcagtg


 


tgcagtgaggaaaggggcaaggatcgggaagctgtgtgactgtccccgaggaacctcctgcaattccttcctcctgaagtgcttatga


 


TGFB1 Mouse (nucleic acid sequence):


(SEQ ID NO: 85)



Atgccgccctcggggctgcggctactgccgcttctgctcccactcccgtggcttctagtgctgacgcccgggaggccagccgcggga



 


ctctccacctgcaagaccatcgacatggagctggtgaaacggaagcgcatcgaagccatccgtggccagatcctgtccaaactaagg


 


ctcgccagtcccccaagccagggggaggtaccgcccggcccgctgcccgaggcggtgctcgctttgtacaacagcacccgcgacc


 


gggtggcaggcgagagcgccgacccagagccggagcccgaagcggactactatgctaaagaggtcacccgcgtgctaatggtgga


 


ccgcaacaacgccatctatgagaaaaccaaagacatctcacacagtatatatatgttcttcaatacgtcagacattcgggaagcagtgcc


 


cgaacccccattgctgtcccgtgcagagctgcgcttgcagagattaaaatcaagtgtggagcaacatgtggaactctaccagaaatata


 


gcaacaattcctggcgttaccttggtaaccggctgctgacccccactgatacgcctgagtggctgtcttttgacgtcactggagttgtacg


 


gcagtggctgaaccaaggagacggaatacagggctttcgattcagcgctcactgctcttgtgacagcaaagataacaaactccacgtg


 


gaaatcaacgggatcagccccaaacgtcggggcgacctgggcaccatccatgacatgaaccggcccttcctgctcctcatggccacc


 


cccctggaaagggcccagcacctgcacagctcacggcaccggagagccctggataccaactattgcttcagctccacagagaagaa


 


ctgctgtgtgcggcagctgtacattgactttaggaaggacctgggttggaagtggatccacgagcccaagggctaccatgccaacttct


 


gtctgggaccctgcccctatatttggagcctggacacacagtacagcaaggtccttgccctctacaaccaacacaacccgggcgcttcg


 


gcgtcaccgtgctgcgtgccgcaggctttggagccactgcccatcgtctactacgtgggtcgcaagcccaaggtggagcagttgtcca


 


acatgattgtgcgctcctgcaagtgcagctga


 


TGFB1 Human (nucleic acid sequence):


(SEQ ID NO: 86)



Atgccgccctccgggctgcggctgctgccgctgctgctaccgctgctgtggctactggtgctgacgcctggccggccggccgcggg



 


actatccacctgcaagactatcgacatggagctggtgaagcggaagcgcatcgaggccatccgcggccagatcctgtccaagctgcg


 


gctcgccagccccccgagccagggggaggtgccgcccggcccgctgcccgaggccgtgctcgccctgtacaacagcacccgcga


 


ccgggtggccggggagagtgcagaaccggagcccgagcctgaggccgactactacgccaaggaggtcacccgcgtgctaatggtg


 


gaaacccacaacgaaatctatgacaagttcaagcagagtacacacagcatatatatgttcttcaacacatcagagctccgagaagcggt


 


acctgaacccgtgttgctctcccgggcagagctgcgtctgctgaggctcaagttaaaagtggagcagcacgtggagctgtaccagaaa


 


tacagcaacaattcctggcgatacctcagcaaccggctgctggcacccagcgactcgccagagtggttatcttttgatgtcaccggagtt


 


gtgcggcagtggttgagccgtggaggggaaattgagggctttcgccttagcgcccactgctcctgtgacagcagggataacacactgc


 


aagtggacatcaacgggttcactaccggccgccgaggtgacctggccaccattcatggcatgaaccggcctttcctgcttctcatggcc


 


accccgctggagagggcccagcatctgcaaagctcccggcaccgccgagccctggacaccaactattgcttcagctccacggagaa


 


gaactgctgcgtgcggcagctgtacattgacttccgcaaggacctcggctggaagtggatccacgagcccaagggctaccatgccaa


 


cttctgcctcgggccctgcccctacatttggagcctggacacgcagtacagcaaggtcctggccctgtacaaccagcataacccgggc


 


gcctcggcggcgccgtgctgcgtgccgcaggcgctggagccgctgcccatcgtgtactacgtgggccgcaagcccaaggtggagc


 


agctgtccaacatgatcgtgcgctcctgcaagtgcagctga


 


TGFB2 Mouse (nucleic acid sequence):


(SEQ ID NO: 87)



Atgcactactgtgtgctgagcacctttttgctcctgcatctggtcccggtggcgctcagtctgtctacctgcagcaccctcgacatggatc



 


agtttatgcgcaagaggatcgaggccatccgcgggcagatcctgagcaagctgaagctcaccagccccccggaagactatccggag


 


ccggatgaggtccccccggaggtgatttccatctacaacagtaccagggacttactgcaggagaaggcaagccggagggcagccgc


 


ctgcgagcgcgagcggagcgacgaggagtactacgccaaggaggtttataaaatcgacatgccgtcccacctcccctccgaaaatgc


 


catcccgcccactttctacagaccctacttcagaatcgtccgctttgatgtctcaacaatggagaaaaatgcttcgaatctggtgaaggca


 


gagttcagggtcttccgcttgcaaaaccccaaagccagagtggccgagcagcggattgaactgtatcagatccttaaatccaaagactt


 


aacatctcccacccagcgctacatcgatagcaaggttgtgaaaaccagagcggagggtgaatggctctccttcgacgtgacagacgct


 


gtgcaggagtggcttcaccacaaagacaggaacctggggtttaaaataagtttacactgcccctgctgtaccttcgtgccgtctaataatt


 


acatcatcccgaataaaagcgaagagctcgaggcgagatttgcaggtattgatggcacctctacatatgccagtggtgatcagaaaact


 


ataaagtccactaggaaaaaaaccagtgggaagaccccacatctcctgctaatgttgttgccctcctacagactggagtcacaacagtcc


 


agccggcggaagaagcgcgctttggatgctgcctactgctttagaaatgtgcaggataattgctgccttcgccctctttacattgattttaa


 


gagggatcttggatggaaatggatccatgaacccaaagggtacaatgctaacttctgtgctggggcatgcccatatctatggagttcaga


 


cactcaacacaccaaagtcctcagcctgtacaacaccataaatcccgaagcttccgcttccccttgctgtgtgtcccaggatctggaacc


 


actgaccattctctattacattggaaatacgcccaagatcgaacagctttccaatatgattgtcaagtcttgtaaatgcagctaa


 


TGFB2 Human (nucleic acid sequence):


(SEQ ID NO: 88)



Atgcactactgtgtgctgagcgcttttctgatcctgcatctggtcacggtcgcgctcagcctgtctacctgcagcacactcgatatggacc



 


agttcatgcgcaagaggatcgaggcgatccgcgggcagatcctgagcaagctgaagctcaccagtcccccagaagactatcctgagc


 


ccgaggaagtccccccggaggtgatttccatctacaacagcaccagggacttgctccaggagaaggcgagccggagggcggccgc


 


ctgcgagcgcgagaggagcgacgaagagtactacgccaaggaggtttacaaaatagacatgccgcccttcttcccctccgaaactgtc


 


tgcccagttgttacaacaccctctggctcagtgggcagcttgtgctccagacagtcccaggtgctctgtgggtaccttgatgccatcccgc


 


ccactttctacagaccctacttcagaattgttcgatttgacgtctcagcaatggagaagaatgcttccaatttggtgaaagcagagttcaga


 


gtctttcgtttgcagaacccaaaagccagagtgcctgaacaacggattgagctatatcagattctcaagtccaaagatttaacatctccaac


 


ccagcgctacatcgacagcaaagttgtgaaaacaagagcagaaggcgaatggctctccttcgatgtaactgatgctgttcatgaatggct


 


tcaccataagacaggaacctgggatttaaaataagcttacactgtccctgctgcacttttgtaccatctaataattacatcatcccaaataa


 


aagtgaagaactagaagcaagatttgcaggtattgatggcacctccacatataccagtggtgatcagaaaactataaagtccactaggaa


 


aaaaaacagtgggaagaccccacatctcctgctaatgttattgccctcctacagacttgagtcacaacagaccaaccggcggaagaag


 


cgtgctttggatgcggcctattgctttagaaatgtgcaggataattgctgcctacgtccactttacattgatttcaagagggatctagggtgg


 


aaatggatacacgaacccaaagggtacaatgccaacttctgtgctggagcatgcccgtatttatggagttcagacactcagcacagcag


 


ggtcctgagcttatataataccataaatccagaagcatctgcttctccttgctgcgtgtcccaagatttagaacctctaaccattctctactac


 


attggcaaaacacccaagattgaacagctttctaatatgattgtaaagtcttgcaaatgcagctaa


 


TGFB3 Mouse (nucleic acid sequence):


(SEQ ID NO: 89)



Atgaagatgcacttgcaaagggctctggtagtcctggccctgctgaacttggccacaatcagcctctctctgtccacttgcaccacgttg



 


gacttcggccacatcaagaagaagagggtggaagccattaggggacagatcttgagcaagctcaggctcaccagcccccctgagcc


 


atcggtgatgacccacgtcccctatcaggtcctggcactttacaacagcacccgggagttgctggaagagatgcacggggagaggga


 


ggaaggctgcactcaggagacctcggagtctgagtactatgccaaagagatccataaattcgacatgatccagggactggcggagca


 


caatgaactggccgtctgccccaaaggaattacctctaaggtttttcgtttcaatgtgtcctcagtggagaaaaatggaaccaatctgttcc


 


gggcagagttccgggtcttgcgggtgcccaaccccagctccaagcgcacagagcagagaattgagctcttccagatacttcgaccgg


 


atgagcacatagccaagcagcgctacataggtggcaagaatctgcccacaaggggcaccgctgaatggctgtctttcgatgtcactga


 


cactgtgcgcgagtggctgttgaggagagagtccaacttgggtctggaaatcagcatccactgtccatgtcacacctttcagcccaatgg


 


agacatactggaaaatgttcatgaggtgatggaaatcaaattcaaaggagtggacaatgaagatgaccatggccgtggagacctgggg


 


cgtctcaagaagcaaaaggatcaccacaacccacacctgatcctcatgatgatccccccacaccgactggacagcccaggccagggc


 


agtcagaggaagaagagggccctggacaccaattactgcttccgcaacctggaggagaactgctgtgtacgcccctttatattgacttc


 


cggcaggatctaggctggaaatgggtccacgaacctaagggttactatgccaacttctgctcaggcccttgcccatacctccgcagcgc


 


agacacaacccatagcacggtgcttggactatacaacaccctgaacccagaggcgtctgcctcgccatgctgcgtcccccaggacctg


 


gagcccctgaccatcttgtactatgtgggcagaacccccaaggtggagcagctgtccaacatggtggtgaagtcgtgtaagtgcagctg


 


a


 


TGFB3 Human (nucleic acid sequence):


(SEQ ID NO: 90)



Atgaagatgcacttgcaaagggctctggtggtcctggccctgctgaactttgccacggtcagcctctctctgtccacttgcaccaccttg



 


gacttcggccacatcaagaagaagagggtggaagccattaggggacagatcttgagcaagctcaggctcaccagcccccctgagcc


 


aacggtgatgacccacgtcccctatcaggtcctggccctttacaacagcacccgggagctgctggaggagatgcatggggagaggga


 


ggaagcctgcacccaggaaaacaccgagtcggaatactatgccaaagaaatccataaattcgacatgatccaggggctggcggagc


 


acaacgaactggctgtctgccctaaaggaattacctccaaggttttccgcttcaatgtgtcctcagtggagaaaaatagaaccaacctatt


 


ccgagcagaattccgggtcttgcgggtgcccaaccccagctctaagcggaatgagcagaggatcgagctcttccagatccttcggcca


 


gatgagcacattgccaaacagcgctatatcggtggcaagaatctgcccacacggggcactgccgagtggctgtcctttgatgtcactga


 


cactgtgcgtgagtggctgttgagaagagagtccaacttaggtctagaaatcagcattcactgtccatgtcacacctttcagcccaatgga


 


gatatcctggaaaacattcacgaggtgatggaaatcaaattcaaaggcgtggacaatgaggatgaccatggccgtggagatctggggc


 


gcctcaagaagcagaaggatcaccacaaccctcatctaatcctcatgatgattcccccacaccggctcgacaacccgggccaggggg


 


gtcagaggaagaagcgggctttggacaccaattactgcttccgcaacttggaggagaactgctgtgtgcgccccctctacattgacttcc


 


gacaggatctgggctggaagtgggtccatgaacctaagggctactatgccaacttctgctcaggcccttgcccatacctccgcagtgca


 


gacacaacccacagcacggtgctgggactgtacaacactctgaaccctgaagcatctgcctcgccttgctgcgtgccccaggacctgg


 


agcccctgaccatcctgtactatgttgggaggacccccaaagtggagcagctctccaacatggtggtgaagtcttgtaaatgtagctga


 


PDGF alpha Mouse (nucleic acid sequence):


(SEQ ID NO: 91)



Atgaggacctgggcttgcctgctgctcctcggctgcggatacctcgcccatgccctggccgaggaagccgagataccccgggagttg



 


atcgagcggctggctcgaagtcagatccacagcatccgggacctccagcgactcttggagatagactccgtaggggctgaggatgcc


 


ttggagacaagtctgagagcccatgggtcccatgccattaaccatgtgcccgagaagcggcctgtgcccattcgcaggaagagaagta


 


ttgaggaagccattcctgcagtttgcaagaccaggacggtcatttacgagatacctcggagccaggtggaccccacatcggccaacttc


 


ctgatctggcccccatgtgtggaggtgaagcgctgcactggctgttgtaacaccagcagcgtcaagtgccaagccttcacgggtccacc


 


accgcagtgtcaaggtggccaaagtggagtatgtcaggaagaagccaaaattgaaagaggtccaggtgaggttagaggaacacctg


 


gagtgtgcatgtgcgacctccaacctgaacccagaccatcgggaggaggagacagatgtgaggtga


 


PDGF alpha Human (nucleic acid sequence):


(SEQ ID NO: 92)



Atgaggaccttggcttgcctgctgctcctcggctgcggatacctcgcccatgttctggccgaggaagccgagatcccccgcgaggtga



 


tcgagaggctggcccgcagtcagatcccagcatccgggacctccagcgactcctggagatagactccgtagggagtgaggattcttt


 


ggacaccagcctgagagctcacggggtccatgccactaagcatgtgcccgagaagcggcccctgcccattcggaggaagagaagc


 


atcgaggaagctgtccccgctgtctgcaagaccaggacggtcatttacgagattcctcggagtcaggtcgaccccacgtccgccaactt


 


cctgatctggcccccgtgcgtggaggtgaaacgctgcaccggctgctgcaacacgagcagtgtcaagtgccagccctcccgcgtcca


 


ccaccgcagcgtcaaggtggccaaggtggaatacgtcaggaagaagccaaaattaaaagaagtccaggtgaggttagaggagcattt


 


ggagtgcgcctgcgcgaccacaagcctgaatccggattatcgggaagaggacacgggaaggcctagggagtcaggtaaaaaacgg


 


aaaagaaaaaggttaaaacccacctaa


 


Brain derived neurotrophic factor (BDNF) Mouse (nucleic acid sequence):


(SEQ ID NO: 93)



Atgttccaccaggtgagaagagtgatgaccatccttttccttactatggttatttcatacttcggttgcatgaaggcggcgcccatgaaaga



 


agtaaacgtccacggacaaggcaacttggcctacccaggtgtgcggacccatgggactctggagagcgtgaatgggcccagggcag


 


gttcgagaggtctgacgacgacatcactggctgacacttttgagcacgtcatcgaagagctgctggatgaggaccagaaggttcggcc


 


caacgaagaaaaccataaggacgcggacttgtacacttcccgggtgatgctcagcagtcaagtgcctttggagcctcctctactctttctg


 


ctggaggaatacaaaaattacctggatgccgcaaacatgtctatgagggttcggcgccactccgaccctgcccgccgtggggagctga


 


gcgtgtgtgacagtattagcgagtgggtcacagcggcagataaaaagactgcagtggacatgtctggcgggacggtcacagtcctag


 


agaaagtcccggtatccaaaggccaactgaagcagtatttctacgagaccaagtgtaatcccatgggttacaccaaggaaggctgcag


 


gggcatagacaaaaggcactggaactcgcaatgccgaactacccaatcgtatgttcgggcccttactatggatagcaaaaagagaattg


 


gctggcgattcataaggatagacacttcctgtgtatgtacactgaccattaaaaggggaagatag


 


Brain derived neurotrophic factor (BDNF) Human (nucleic acid sequence):


(SEQ ID NO: 94)



Atgaccatccttttccttactatggttatttcatactttggttgcatgaaggctgcccccatgaaagaagcaaacatccgaggacaaggtgg



 


cttggcctacccaggtgtgcggacccatgggactctggagagcgtgaatgggcccaaggcaggttcaagaggcttgacatcattggct


 


gacactttcgaacacgtgatagaagagctgttggatgaggaccagaaagttcggcccaatgaagaaaacaataaggacgcagacttgt


 


acacgtccagggtgatgctcagtagtcaagtgcctttggagcctcctcttctctttctgctggaggaatacaaaaattacctagatgctgca


 


aacatgtccatgagggtccggcgccactctgaccctgcccgccgagggggagctgagcgtgtgtgacagtattagtgagtgggtaacg


 


gcggcagacaaaaagactgcagtggacatgtcgggcgggacggtcacagtccttgaaaaggtccctgtatcaaaaggccaactgaa


 


gcaatacttctacgagaccaagtgcaatcccatgggttacacaaaagaaggctgcaggggcatagacaaaaggcattggaactccca


 


gtgccgaactacccagtcgtacgtgcgggcccttaccatggatagcaaaaagagaattggctggcgattcataaggatagacacttctt


 


gtgtatgtacattgaccattaaaaggggaagatag


 


Beta nerve growth factor Mouse (nucleic acid sequence):


(SEQ ID NO: 95)



Atgtccatgttgttctacactctgatcactgcgtttttgatcggcgtacaggcagaaccgtacacagatagcaatgtcccagaaggagact



 


ctgtccctgaagcccactggactaaacttcagcattcccttgacacagccctccgcagagcccgcagtgcccctactgcaccaatagct


 


gcccgagtgacagggcagacccgcaacatcactgtagaccccagactgtttaagaaacggagactccactcaccccgtgtgctgttca


 


gcacccagcctccacccacctcttcagacactctggatctagacttccaggcccatggtacaatccctttcaacaggactcaccggagc


 


aagcgctcatccacccacccagtcttccacatgggggagttctcagtgtgtgacagtgtcagtgtgtgggttggagataagaccacagc


 


cacagacatcaagggcaaggaggtgacagtgctggccgaggtgaacattaacaacagtgtattcagacagtacttttttgagaccaagt


 


gccgagcctccaatcctgttgagagtgggtgccggggcatcgactccaaacactggaactcatactgcaccacgactcacaccttcgtc


 


aaggcgttgacaacagatgagaagcaggctgcctggaggttcatccggatagacacagcctgtgtgtgtgtgctcagcaggaaggcta


 


caagaagaggctga


 


Beta nerve growth factor Human (nucleic acid sequence):


(SEQ ID NO: 96)



Atgtccatgttgttctacactctgatcacagcttttctgatcggcatacaggcggaaccacactcagagagcaatgtccctgcaggacac



 


accatcccccaagcccactggactaaacttcagcattcccttgacactgcccttcgcagagcccgcagcgccccggcagcggcgata


 


gctgcacgcgtggcggggcagacccgcaacattactgtggaccccaggctgtttaaaaagcggcgactccgttcaccccgtgtgctgt


 


ttagcacccagcctccccgtgaagctgcagacactcaggatctggacttcgaggtcggtggtgctgcccccttcaacaggactcacag


 


gagcaagcggtcatcatcccatcccatcttccacaggggcgaattctcggtgtgtgacagtgtcgcgtgtgggttggggataagacca


 


ccgccacagacatcaagggcaaggaggtgatggtgttgggagaggtgaacattaacaacagtgtattcaaacagtacttttttgagacc


 


aagtgccgggacccaaatcccgttgacagcgggtgccggggcattgactcaaagcactggaactcatattgtaccacgactcacacctt


 


tgtcaaggcgctgaccatggatggcaagcaggctgcctggcggtttatccggatagatacggcctgtgtgtgtgtgctcagcaggaag


 


gctgtgagaagagcctga


 


Albumin Mouse (nucleic acid sequence):


(SEQ ID NO: 97)



Atgaagtgggtaacctttctcctcctcctcttcgtctccggctctgctttttccaggggtgtgtttcgccgagaagcacacaagagtgagat



 


cgcccatcggtataatgatttgggagaacaacatttcaaaggcctagtcctgattgccttttcccagtatctccagaaatgctcatacgatg


 


agcatgccaaattagtgcaggaagtaacagactttgcaaagacgtgtgttgccgatgagtctgccgccaactgtgacaaatcccttcaca


 


ctctttttggagataagttgtgtgccattccaaacctccgtgaaaactatggtgaactggctgactgctgtacaaaacaagagcccgaaag


 


aaacgaatgtttcctgcaacacaaagatgacaaccccagcctgccaccatttgaaaggccagaggctgaggccatgtgcacctccttta


 


aggaaaacccaaccacctttatgggacactatttgcatgaagttgccagaagacatccttatttctatgccccagaacttctttactatgctg


 


agcagtacaatgagattctgacccagtgttgtgcagaggctgacaaggaaagctgcctgaccccgaagcttgatggtgtgaaggagaa


 


agcattggtctcatctgtccgtcagagaatgaagtgctccagtatgcagaagtttggagagagagcttttaaagcatgggcagtagctcgt


 


ctgagccagacattccccaatgctgactttgcagaaatcaccaaattggcaacagacctgaccaaagtcaacaaggagtgctgccatgg


 


tgacctgctggaatgcgcagatgacagggcggaacttgccaagtacatgtgtgaaaaccaggcgactatctccagcaaactgcagact


 


tgctgcgataaaccactgttgaagaaagcccactgtcttagtgaggtggagcatgacaccatgcctgctgatctgcctgccattgctgctg


 


attttgttgaggaccaggaagtgtgcaagaactatgctgaggccaaggatgtcttcctgggcacgttcttgtatgaatattcaagaagaca


 


ccctgattactctgtatccctgttgctgagacttgctaagaaatatgaagccactctggaaaagtgctgcgctgaagccaatcctcccgcat


 


gctacggcacagtgcttgctgaatttcagcctcttgtagaagagcctaagaacttggtcaaaaccaactgtgatctttacgagaagcttgg


 


agaatatggattccaaaatgccattctagttcgctacacccagaaagcacctcaggtgtcaaccccaactctcgtggaggctgcaagaa


 


acctaggaagagtgggcaccaagtgttgtacacttcctgaagatcagagactgccttgtgtggaagactatctgtctgcaatcctgaacc


 


gtgtgtgtctgctgcatgagaagaccccagtgagtgagcatgttaccaagtgctgtagtggatccctggtggaaaggcggccatgcttct


 


ctgctctgacagttgatgaaacatatgtccccaaagagtttaaagctgagaccttcaccttccactctgatatctgcacacttccagagaag


 


gagaagcagattaagaaacaaacggctcttgctgagctggtgaagcacaagcccaaggctacagcggagcaactgaagactgtcatg


 


gatgactttgcacagttcctggatacatgttgcaaggctgctgacaaggacacctgcttctcgactgagggtccaaaccttgtcactagat


 


gcaaagacgccttagcctaa


 


Albumin Human (nucleic acid sequence):


(SEQ ID NO: 98)



atgaagtgggtaacctttatttcccttctttttctctttagctcggcttattccaggggtgtgtttcgtcgagatgcacacaagagtgaggttgc



 


tcatcggtttaaagatttgggagaagaaaatttcaaagccttggtgttgattgcctttgctcagtatcttcagcagtgtccatttgaagatcatg


 


taaaattagtgaatgaagtaactgaatttgcaaaaacatgtgttgctgatgagtcagctgaaaattgtgacaaatcacttcataccctttttgg


 


agacaaattatgcacagttgcaactcttcgtgaaacctatggtgaaatggctgactgctgtgcaaaacaagaacctgagagaaatgaatg


 


cttcttgcaacacaaagatgacaacccaaacctcccccgattggtgagaccagaggttgatgtgatgtgcactgcttttcatgacaatgaa


 


gagacatttttgaaaaaatacttatatgaaattgccagaagacatccttacttttatgccccggaactccttttctttgctaaaaggtataaagc


 


tgcttttacagaatgttgccaagctgctgataaagctgcctgcctgttgccaaagctcgatgaacttcgggatgaagggaaggcttcgtct


 


gccaaacagagactcaagtgtgccagtctccaaaaatttggagaaagagctttcaaagcatgggcagtagctcgcctgagccagag at


 


ttcccaaagctgagtttgcagaagtttccaagttagtgacagatcttaccaaagtccacacggaatgctgccatggagatctgcttgaatgt


 


gctgatgacagggcggaccttgccaagtatatctgtgaaaatcaagattcgatctccagtaaactgaaggaatgctgtgaaaaacctctg


 


ttggaaaaatcccactgcattgccgaagtggaaaatgatgagatgcctgctgacttgccttcattagctgctgattttgttgaaagtaaggat


 


gtttgcaaaaactatgctgaggcaaaggatgtcttcctgggcatgtttttgtatgaatatgcaagaaggcatcctgattactctgtcgtgctg


 


ctgctgagacttgccaagacatatgaaaccactctagagaagtgctgtgccgctgcagatcctcatgaatgctatgccaaagtgttcgat


 


gaatttaaacctcttgtggaagagcctcagaatttaatcaaacaaaattgtgagctttttgagcagcttggagagtacaaattccagaatgc


 


gctattagttcgttacaccaagaaagtaccccaagtgtcaactccaactcttgtagaggtctcaagaaacctaggaaaagtgggcagcaa


 


atgttgtaaacatcctgaagcaaaaagaatgccctgtgcagaagactatctatccgtggtcctgaaccagttatgtgtgttgcatgagaaa


 


acgccagtaagtgacagagtcaccaaatgctgcacagaatccttggtgaacaggcgaccatgcttttcagctctggaagtcgatgaaac


 


atacgttcccaaagagtttaatgctgaaacattcaccttccatgcagatatatgcacactttctgagaaggagagacaaatcaagaaacaa


 


actgcacttgttgagctcgtgaaacacaagcccaaggcaacaaaagagcaactgaaagctgttatggatgatttcgcagcttttgtagag


 


aagtgctgcaaggctgacgataaggagacctgctttgccgaggagggtaaaaaacttgttgctgcaagtcaagctgccttaggcttataa


 


Calcitonin Mouse (nucleic acid sequence):


(SEQ ID NO: 99)



Atgggcttcctgaagttctcccctttcctggttgtcagcatcttgctcctgtaccaggcatgcagcctccaggcagtgcctttgaggtcaat



 


cttggaaagcagcccaggcatggccactctcagtgaagaagaagttcgcctgctggctgcactggtgcaggactatatgcagatgaaa


 


gccagggagctggagcaggaggaagagcaggaggctgagggctctagcttggacagccccagatctaagcggtgtgggaatctga


 


gtacctgcatgctgggcacgtacacacaagacctcaacaagtttcacaccttcccccaaacttcaattggggttgaagcacctggcaag


 


aaaagggatgtggccaaggacttggagacaaaccaccaatcccattttggcaactaa


 


Calcitonin Human (nucleic acid sequence):


(SEQ ID NO: 100)



Atgggcttccaaaagttctcccccttcctggctctcagcatcttggtcctgttgcaggcaggcagcctccatgcagcaccattcaggtctg



 


ccctggagagcagcccagcagacccggccacgctcagtgaggacgaagcgcgcctcctgctggctgcactggtgcaggactatgtg


 


cagatgaaggccagtgagctggagcaggagcaagagagagagggctccagcctggacagccccagatctaagcggtgcggtaatc


 


tgagtacttgcatgctgggcacatacacgcaggacttcaacaagtttcacacgttcccccaaactgcaattggggttggagcacctggaa


 


agaaaagggatatgtccagcgacttggagagagaccatcgccctcatgttagcatgccccagaatgccaactaa






Exemplary cleavage sites that can be used for the constructs of the present invention include, but are not limited to, aagagg (SEQ ID NO: 101); aagcgt (SEQ ID NO: 102); aagcgc (SEQ ID NO: 103); aaaaga (SEQ ID NO: 104); aagaggagg (SEQ ID NO: 105); aagaga (SEQ ID NO: 106); cgcaaa (SEQ ID NO: 107); cggcgg (SEQ ID NO: 108); tatctg (SEQ ID NO: 109); aggcgg (SEQ ID NO: 110); cggagc (SEQ ID NO: 111); cggtct (SEQ ID NO: 112); cgaagc (SEQ ID NO: 113); aaacgg (SEQ ID NO: 114); aagagaggt (SEQ ID NO: 115); aaacgaggc (SEQ ID NO: 116); gggccgccgc (SEQ ID NO: 117); ccacgagct (SEQ ID NO: 118); ccccgagct (SEQ ID NO: 119); cgaaggcagctgcgggct (SEQ ID NO: 120); cgtaggcagctgagggta (SEQ ID NO: 121); cgccgcagt (SEQ ID NO: 122); cgaaga (SEQ ID NO: 123); cggaga (SEQ ID NO: 124); agaagg (SEQ ID NO: 125); aaacgc (SEQ ID NO: 126); tccagcattcggagg (SEQ ID NO: 127); agcagtcggagg (SEQ ID NO: 128); gagagggac (SEQ ID NO: 129); cggcgc (SEQ ID NO: 130); aggcgc (SEQ ID NO: 131); cggggcaccaag (SEQ ID NO: 132); caccgc (SEQ ID NO: 133); acccgtgtc (SEQ ID NO: 134); tcgcgcatt (SEQ ID NO: 135); cgacgg (SEQ ID NO: 136); aagagaaga (SEQ ID NO: 137); and ggccgccgc (SEQ ID NO: 138).
One skilled in the art would know how to design a cleavage site, so the propeptide and bioluminescent protein will separate and function normally.
Any luciferase known in the art, any propeptide known in the art and any cleavage site known in the art can be used to make a fusion protein according to the method of the present invention.
Exemplary luciferase fusion proteins (their nucleic acid sequences) include, but are not limited to, the sequences below. Underline indicates the putative cleavage sites. Italics indicates Gaussia luciferase without its signal peptide.












Proamylin Mouse (nucleic acid sequence):


(SEQ ID NO: 139)


Atgatgtgcatctccaaactgccagctgtcctcctcatcctctctgtggc


actgaaccacttgagagctacacctgtcagaagtggtagcaaccctcaga


tggacaaacggaagtgcaacacggccacgtgtgccacacaacgcctggca


aactttttggttcgttccagcaacaaccttggtccagtcctcccaccaac


caacgtgggatcgaatacatatggcaagaggaatgcgaagcccaccgaga


acaacgaagacttcaacatcgtggccgtggccagcaacttcgcgaccacg


gatctcgatgctgaccgcgggaagttgcccggcaagaagctgccgctgga


ggtgctcaaagagatggaagccaatgcccggaaagctggctgcaccaggg


gctgtctgatctgcctgtcccacatcaagtgcacgcccaagatgaagaag


ttcatcccaggacgctgccacacctacgaaggcgacaaagagtccgcaca


gggcggcataggcgaggcgatcgtcgacattcctgagattcctgggttca


aggacttggagcccatggagcagttcatcgcacaggtcgatctgtgtgtg


gactgcacaactggctgcctcaaagggcttgccaacgtgcagtgttctga


cctgctcaagaagtggctgccgcaacgctgtgcgacctttgccagcaaga


tccagggccaggtggacaagatcaagggggccggtggtgacaagaggaat


gcggcaggggatccaaatagggaatccttggatttcttactcgtttaa


 


Proamylin Human (nucleic acid sequence):


(SEQ ID NO: 140)


Atgggcatcctgaagctgcaagtatttctcattgtgctctctgttgcatt


gaaccatctgaaagctacacccattgaaagtcatcaggtggaaaagcgga


aatgcaacactgccacatgtgcaacgcagcgcctggcaaattttttagtt


cattccagcaacaactttggtgccattctctcatctaccaacgtgggatc


caatacatatggcaagaggaatgcaaagcccaccgagaacaacgaagact


tcaacatcgtggccgtggccagcaacttcgcgaccacggatctcgatgct


gaccgcgggaagttgcccggcaagaagctgccgctggaggtgctcaaaga


gatggaagccaatgcccggaaagctggctgcaccaggggctgtctgatct


gcctgtcccacatcaagtgcacgcccaagatgaagaagttcatcccagga


cgctgccacacctacgaaggcgacaaagagtccgcacagggcggcatagg


cgaggcgatcgtcgacattcctgagattcctgggttcaaggacttggagc


ccatggagcagttcatcgcacaggtcgatctgtgtgtggactgcacaact


ggctgcctcaaagggcttgccaacgtgcagtgttctgacctgctcaagaa


gtggctgccgcaacgctgtgcgacctttgccagcaagatccagggccagg


tggacaagatcaagggggccggtggtgacaagaggaatgcggtagaggtt


ttaaagagagagccactgaattacttgcccctttag


 


Proinsulin Mouse (nucleic acid sequence):


(SEQ ID NO: 141)


Atggccctgtggatgcgcttcctgcccctgctggccctgctcttcctctg


ggagtcccaccccacccaggcttttgtcaagcagcacctttgtggttccc


acctggtggaggctctctacctggtgtgtggggagcgtggcttcttctac


acacccatgtcccgccgtgaagtggaggacccacaagtggcacaactgga


gctgggtggaggcccgggagcaggtgaccttcagaccttggcactggagg


tggcccagcagaagaggaagcccaccgagaacaacgaagacttcaacatc


gtggccgtggccagcaacttcgcgaccacggatctcgatgctgaccgcgg


gaagttgcccggcaagaagctgccgctggaggtgctcaaagagatggaag


ccaatgcccggaaagctggctgcaccaggggctgtctgatctgcctgtcc


cacatcaagtgcacgcccaagatgaagaagttcatcccaggacgctgcca


cacctacgaaggcgacaaagagtccgcacagggcggcataggcgaggcga


tcgtcgacattcctgagattcctgggttcaaggacttggagcccatggag


cagttcatcgcacaggtcgatctgtgtgtggactgcacaactggctgcct


caaagggcttgccaacgtgcagtgttctgacctgctcaagaagtggctgc


cgcaacgctgtgcgacctttgccagcaagatccagggccaggtggacaag


atcaagggggccggtggtgacaagcgtggcattgtagatcagtgctgcac


cagcatctgctccctctaccagctggagaactactgcaactag


 


Proinsulin Human (nucleic acid sequence):


(SEQ ID NO: 142)


Atggccctgtggatgcgcctcctgcccctgctggcgctgctggccctctg


gggacctgacccagccgcagcctttgtgaaccaacacctgtgcggctcac


acctggtggaagctctctacctagtgtgcggggaacgaggcttcttctac


acacccaagacccgccgggaggcagaggacctgcaggtggggcaggtgga


gctgggcgggggccctggtgcaggcagcctgcagcccttggccctggagg


ggtccctgcagaagaggaagcccaccgagaacaacgaagacttcaacatc


gtggccgtggccagcaacttcgcgaccacggatctcgatgctgaccgcgg


gaagttgcccggcaagaagctgccgctggaggtgctcaaagagatggaag


ccaatgcccggaaagctggctgcaccaggggctgtctgatctgcctgtcc


cacatcaagtgcacgcccaagatgaagaagttcatcccaggacgctgcca


cacctacgaaggcgacaaagagtccgcacagggcggcataggcgaggcga


tcgtcgacattcctgagattcctgggttcaaggacttggagcccatggag


cagttcatcgcacaggtcgatctgtgtgtggactgcacaactggctgcct


caaagggcttgccaacgtgcagtgttctgacctgctcaagaagtggctgc


cgcaacgctgtgcgacctttgccagcaagatccagggccaggtggacaag


atcaagggggccggtggtgacaagcgtggcattgtggaacaatgctgtac


cagcatctgctccctctaccagctggagaactactgcaactag


 


Proglucagon (includes GRPP, glucagon. GLP-1,


GLP-2) Mouse (nucleic acid sequence):


(SEQ ID NO: 143)


Atgaagaccatttactttgtggctggattgcttataatgctggtgcaagg


cagctggcagcacgcccttcaagacacagaggagaaccccagatcattcc


cagcttcccagacagaagcgcatgaggaccctgatgagatgaatgaagac


aaacgccactcacagggcacattcaccagcgactacagcaaatacctgga


ctcccgccgtgcccaagattttgtgcagtggttgatgaacaccaagagga


accggaacaacattgccaaacgtcatgatgaatttgagaggcatgctgaa


gggaccatttaccagtgatgtgagttcttacttggagggccaggcagcaa


aggaattcattgcttggctggtgaaaggccgaggaaggcgagacttccca


gaagaagtcgccattgccgaggaactcggccgaaagagaagaaagcccac


cgagaacaacgaagacttcaacatcgtggccgtggccagcaacttcgcga


ccacggatctcgatgctgaccgcgggaagttgcccggcaagaagctgccg


ctggaggtgctcaaagagatggaagccaatgcccggaaagctggctgcac


caggggctgtctgatctgcctgtcccacatcaagtgcacgcccaagatga


agaagttcatcccaggacgctgccacacctacgaaggcgacaaagagtcc


gcacagggcggcataggcgaggcgatcgtcgacattcctgagattcctgg


gttcaaggacttggagcccatggagcagttcatcgcacaggtcgatctgt


gtgtggactgcacaactggctgcctcaaagggcttgccaacgtgcagtgt


tctgacctgctcaagaagtggctgccgcaacgctgtgcgacctttgccag


caagatccagggccaggtggacaagatcaagggggccggtggtgacaagc


gcggccgcaggcacgctgatggctccttctctgacgagatgagcaccatt


ctggataatcttgccaccagggacttcatcaactggctgattcaaaccaa


gatcactgacaagaaatag


 


Proglucagon (includes GRPP, glucagon, GLP-1,


GLP-2) Human (nucleic acid sequence):


(SEQ ID NO: 144)


atgaaaagcatttactttgtggctggattatttgtaatgctggtacaagg


cagctggcaacgttcccttcaagacacagaggagaaatccagatcattct


cagcttcccaggcagacccactcagtgatcctgatcagatgaacgaggac


aagcgccattcacagggcacattcaccagtgactacagcaagtatctgga


ctccaggcgtgcccaagattttgtgcagtggttgatgaataccaagagga


acaggaataacattgccaaacgtcacgatgaatttgagagacatgctgaa


gggacctttaccagtgatgtaagttcttatttggaaggccaagctgccaa


ggaattcattgcttggctggtgaaaggccgaggaaggcgagatttcccag


aagaggtcgccattgttgaagaacttggccgaaagagaagaaagcccacc


gagaacaacgaagacttcaacatcgtggccgtggccagcaacttcgcgac


cacggatctcgatgctgaccgcgggaagttgcccggcaagaagctgccgc


tggaggtgctcaaagagatggaagccaatgcccggaaagctggctgcacc


aggggctgtctgatctgcctgtcccacatcaagtgcacgcccaagatgaa


gaagttcatcccaggacgctgccacacctacgaaggcgacaaagagtccg


cacagggcggcataggcgaggcgatcgtcgacattcctgagattcctggg


ttcaaggacttggagcccatggagcagttcatcgcacaggtcgatctgtg


tgtggactgcacaactggctgcctcaaagggcttgccaacgtgcagtgtt


ctgacctgctcaagaagtggctgccgcaacgctgtgcgacctttgccagc


aagatccagggccaggtggacaagatcaagggggccggtggtgacaagcg


cggccgcagacatgctgatggttctttctctgatgagatgaacaccattc


ttgataatcttgccgccagggactttataaactggttgattcagaccaaa


atcactgacaggaaataa


 


Peptide YY Mouse (nucleic acid sequence):


(SEQ ID NO: 145)


atggtggcggtgcgcaggccttggcccgtcacggtcgcaatgctgctaat


cctgctcgcctgtctgggagccctggtggacgcctaccctgccaaaccag


aggctcccggcgaagacgcctccccggaggagctgagccgctactacgcc


tccctgcgccactacctcaacctggtcacccggcagcggtatggaaaaag


aaagcccaccgagaacaacgaagacttcaacatcgtggccgtggccagca


acttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaag


aagctgccgctggaggtgctcaaagagatggaagccaatgcccggaaagc


tggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacgc


ccaagatgaagaagttcatcccaggacgctgccacacctacgaaggcgac


aaagagtccgcacagggcggcataggcgaggcgatcgtcgacattcctga


gattcctgggttcaaggacttggagcccatggagcagttcatcgcacagg


tcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgccaac


gtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcgac


ctttgccagcaagatccagggccaggtggacaagatcaagggggccggtg


gtgacaagaggagggatgtccccgcagctctgttctccaaactgctcttc


acagacgacagcgacagcgagaacctccccttcaggccagaaggtttgga


ccagtggtga


 


Peptide YY Human (nucleic acid sequence):


(SEQ ID NO: 146)


Atggtgttcgtgcgcaggccgtggcccgccttgaccacagtgcttctggc


cctgctcgtctgcctaggggcgctggtcgacgcctaccccatcaaacccg


aggctcccggcgaagacgcctcgccggaggagctgaaccgctactacgcc


tccctgcgccactacctcaacctggtcacccggcagcggtatgggaaaag


aaagcccaccgagaacaacgaagacttcaacatcgtggccgtggccagca


acttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaag


aagctgccgctggaggtgctcaaagagatggaagccaatgcccggaaagc


tggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacgc


ccaagatgaagaagttcatcccaggacgctgccacacctacgaaggcgac


aaagagtccgcacagggcggcataggcgaggcgatcgtcgacattcctga


gattcctgggttcaaggacttggagcccatggagcagttcatcgcacagg


tcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgccaac


gtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcgac


ctttgccagcaagatccagggccaggtggacaagatcaagggggccggtg


gtgacaagaggagggacggcccggacacgcttctttccaaaacgttcttc


cccgacggcgaggaccgccccgtcaggtcgcggtcggagggcccagacct


gtggtga


 


Neuropeptide Y Mouse (nucleic acid sequence):


(SEQ ID NO: 147)


Atgctaggtaacaagcgaatggggctgtgtggactgaccctcgctctatc


tctgctcgtgtgtttgggcattctggctgaggggtacccctccaagccgg


acaatccgggcgaggacgcgccagcagaggacatggccagatactactcc


gctctgcgacactacatcaatctcatcaccagacagagatatggcaagag


aaagcccaccgagaacaacgaagacttcaacatcgtggccgtggccagca


acttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaag


aagctgccgctggaggtgctcaaagagatggaagccaatgcccggaaagc


tggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacgc


ccaagatgaagaagttcatcccaggacgctgccacacctacgaaggcgac


aaagagtccgcacagggcggcataggcgaggcgatcgtcgacattcctga


gattcctgggttcaaggacttggagcccatggagcagttcatcgcacagg


tcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgccaac


gtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcgac


ctttgccagcaagatccagggccaggtggacaagatcaagggggccggtg


gtgacaagaggaggtccagccctgagacactgatttcagacctcttaatg


aaggaaagcacagaaaacgcccccagaacaaggcttgaagacccttccat


gtggtga


 


Neuropeptide Y Human (nucleic acid sequence):


(SEQ ID NO: 148)


Atgctaggtaacaagcgactggggctgtccggactgaccctcgccctgtc


cctgctcgtgtgcctgggtgcgctggccgaggcgtacccctccaagccgg


acaacccgggcgaggacgcaccagcggaggacatggccagatactactcg


gcgctgcgacactacatcaacctcatcaccaggcagagatatggaaaacg


aaagcccaccgagaacaacgaagacttcaacatcgtggccgtggccagca


acttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaag


aagctgccgctggaggtgctcaaagagatggaagccaatgcccggaaagc


tggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacgc


ccaagatgaagaagttcatcccaggacgctgccacacctacgaaggcgac


aaagagtccgcacagggcggcataggcgaggcgatcgtcgacattcctga


gattcctgggttcaaggacttggagcccatggagcagttcatcgcacagg


tcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgccaac


gtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcgac


ctttgccagcaagatccagggccaggtggacaagatcaagggggccggtg


gtgacaagaggaggtccagcccagagacactgatttcagacctcttgatg


agagaaagcacagaaaatgttcccagaactcggcttgaagaccctgcaat


gtggtga


 


Pancreatic polypeptide Mouse (nucleic acid


sequence):


(SEQ ID NO: 149)


Atggccgtcgcatactgctgcctctccctgtttctcgtatccacttgggt


ggctctgctgctgcagcccctgcaggggacctggggagcccccctggagc


caatgtacccaggcgactatgcgacacctgagcagatggcacaatatgaa


actcagctccgcagatacatcaacacactgaccaggcctaggtatgggaa


gagaaagcccaccgagaacaacgaagacttcaacatcgtggccgtggcca


gcaacttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggc


aagaagctgccgctggaggtgctcaaagagatggaagccaatgcccggaa


agctggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgca


cgcccaagatgaagaagttcatcccaggacgctgccacacctacgaaggc


gacaaagagtccgcacagggcggcataggcgaggcgatcgtcgacattcc


tgagattcctgggttcaaggacttggagcccatggagcagttcatcgcac


aggtcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgcc


aacgtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgc


gacctttgccagcaagatccagggccaggtggacaagatcaagggggccg


gtggtgacaagaggagggccgaggaggagaacacaggtggacttcctgga


gtgcagctctccccctgcaccagccccccagttggcttgattccctgctc


tgcgccctggagctga


 


Pancreatic polypeptide Human (nucleic acid


sequence):


(SEQ ID NO: 150)


Atggctgccgcacgcctctgcctctccctgctgctcctgtccacctgcgt


ggctctgttactacagccactgctgggtgcccagggagccccactggagc


cagtgtacccaggggacaatgccacaccagagcagatggcccagtatgca


gctgatctccgtagatacatcaacatgctgaccaggcctaggtatgggaa


aagaaagcccaccgagaacaacgaagacttcaacatcgtggccgtggcca


gcaacttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggc


aagaagctgccgctggaggtgctcaaagagatggaagccaatgcccggaa


agctggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgca


cgcccaagatgaagaagttcatcccaggacgctgccacacctacgaaggc


gacaaagagtccgcacagggcggcataggcgaggcgatcgtcgacattcc


tgagattcctgggttcaaggacttggagcccatggagcagttcatcgcac


aggtcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgcc


aacgtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgc


gacctttgccagcaagatccagggccaggtggacaagatcaagggggccg


gtggtgacaagaggaggcacaaagaggacacgctggccttctcggagtgg


gggtccccgcatgctgctgtccccagggagctcagcccgctggacttata


a


 


Somatostatin Mouse (nucleic acid sequence):


(SEQ ID NO: 151)


Atgctgtcctgccgtctccagtgcgccctggctgcgctctgcatcgtcct


ggctttgggcggtgtcaccggcgcgccctcggaccccagactccgtcagt


ttctgcagaagtctctggcggctgccaccgggaaacaggaactggccaag


tacttcttggcagagctgcgcaaaaagcccaccgagaacaacgaagactt


caacatcgtggccgtggccagcaacttcgcgaccacggatctcgatgctg


accgcgggaagttgcccggcaagaagctgccgctggaggtgctcaaagag


atggaagccaatgcccggaaagctggctgcaccaggggctgtctgatctg


cctgtcccacatcaagtgcacgcccaagatgaagaagttcatcccaggac


gctgccacacctacgaaggcgacaaagagtccgcacagggcggcataggc


gaggcgatcgtcgacattcctgagattcctgggttcaaggacttggagcc


catggagcagttcatcgcacaggtcgatctgtgtgtggactgcacaactg


gctgcctcaaagggcttgccaacgtgcagtgttctgacctgctcaagaag


tggctgccgcaacgctgtgcgacctttgccagcaagatccagggccaggt


ggacaagatcaagggggccggtggtgaccgcaaactgtccgagcccaacc


agacagagaatgatgccctggagcccgaggatttgccccaggcagctgag


caggacgagatgaggctggagctgcagaggtctgccaactcgaacccagc


aatggcaccccgggaacgcaaagctggctgcaagaacttcttctggaaga


cattcacatcctgttag


 


Somatostatin Human (nucleic acid sequence):


(SEQ ID NO: 152)


Atgctgtcctgccgcctccagtgcgcgctggctgcgctgtccatcgtcct


ggccctgggctgtgtcaccggcgctccctcggaccccagactccgtcagt


ttctgcagaagtccctggctgctgccgcggggaagcaggaactggccaag


tacttcttggcagagctgcgcaaaaagcccaccgagaacaacgaagactt


caacatcgtggccgtggccagcaacttcgcgaccacggatctcgatgctg


accgcgggaagttgcccggcaagaagctgccgctggaggtgctcaaagag


atggaagccaatgcccggaaagctggctgcaccaggggctgtctgatctg


cctgtcccacatcaagtgcacgcccaagatgaagaagttcatcccaggac


gctgccacacctacgaaggcgacaaagagtccgcacagggcggcataggc


gaggcgatcgtcgacattcctgagattcctgggttcaaggacttggagcc


catggagcagttcatcgcacaggtcgatctgtgtgtggactgcacaactg


gctgcctcaaagggcttgccaacgtgcagtgttctgacctgctcaagaag


tggctgccgcaacgctgtgcgacctttgccagcaagatccagggccaggt


ggacaagatcaagggggccggtggtgaccgcaaactgtctgaacccaacc


agacggagaatgatgccctggaacctgaagatctgtcccaggctgctgag


caggatgaaatgaggcttgagctgcagagatctgctaactcaaacccggc


tatggcaccccgagaacgcaaagctggctgcaagaatttcttctggaaga


ctttcacatcctgttag


 


GHRH Mouse (nucleic acid sequence):


(SEQ ID NO: 153)


Atgctgctctgggtgctctttgtgatcctcatcctcaccagtggctccca


ctgctcactgcccccctcacctcccttcaggatgcagcgacacgtagatg


ccatcttcaccaccaactacaggaaactcctgagccagctgtatgcccgg


aaagtgatccaggacatcatgaacaagcaaggggagaggatccaggaaca


aagggccaggctcagccgccaggaagacagcatgtggacagaggacaagc


agatgaccctggagagcatccggcggaagcccaccgagaacaacgaagac


ttcaacatcgtggccgtggccagcaacttcgcgaccacggatctcgatgc


tgaccgcgggaagttgcccggcaagaagctgccgctggaggtgctcaaag


agatggaagccaatgcccggaaagctggctgcaccaggggctgtctgatc


tgcctgtcccacatcaagtgcacgcccaagatgaagaagttcatcccagg


acgctgccacacctacgaaggcgacaaagagtccgcacagggcggcatag


gcgaggcgatcgtcgacattcctgagattcctgggttcaaggacttggag


cccatggagcagttcatcgcacaggtcgatctgtgtgtggactgcacaac


tggctgcctcaaagggcttgccaacgtgcagtgttctgacctgctcaaga


agtggctgccgcaacgctgtgcgacctttgccagcaagatccagggccag


gtggacaagatcaagggggccggtggtgaccggcggttgcagggattccc


aaggatgaagccttcagcggacgcttga


 


GHRH Human (nucleic acid sequence):


(SEQ ID NO: 154)


Atgccactctgggtgttcttctttgtgatcctcaccctcagcaacagctc


ccactgctccccacctccccctttgaccctcaggatgcggcggtatgcag


atgccatcttcaccaacagctaccggaaggtgctgggccagctgtccgcc


cgcaagctgctccaggacatcatgagcaggcagcagggagagagcaacca


agagcgaggagcaagggcacggcttggtcgtcaggtagacagcatgtggg


cagaacaaaagcaaatggaattggagagcatcctggtggccctgcggcgg


aagcccaccgagaacaacgaagacttcaacatcgtggccgtggccagcaa


cttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaaga


agctgccgctggaggtgctcaaagagatggaagccaatgcccggaaagct


ggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacgcc


caagatgaagaagttcatcccaggacgctgccacacctacgaaggcgaca


aagagtccgcacagggcggcataggcgaggcgatcgtcgacattcctgag


attcctgggttcaaggacttggagcccatggagcagttcatcgcacaggt


cgatctgtgtgtggactgcacaactggctgcctcaaagggcttgccaacg


tgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcgacc


tttgccagcaagatccagggccaggtggacaagatcaagggggccggtgg


tgaccggcggctgcagaagcacaggaactcccagggatga


 


POMC (ACTH, MSH) Mouse (nucleic acid sequence):


(SEQ ID NO: 155)


Atgccgagattctgctacagtcgctcaggggccctgttgctggccctcct


gcttcagacctccatagatgtgtggagctggtgcctggagagcagccagt


gccaggacctcaccacggagagcaacctgctggcttgcatccgggcttgc


aaactcgacctctcgctggagacgcccgtgtttcctggcaacggagatga


acagcccctgactgaaaacccccggaagtacgtcatgggtcacttccgct


gggaccgcttcggccccaggaacagcagcagtgctggcagcgcggcgcag


aggcgtgcggaggaagaggcggtgtggggagatggcagtccagagccgag


tccacgcgagggcaagcgcaagcccaccgagaacaacgaagacttcaaca


tcgtggccgtggccagcaacttcgcgaccacggatctcgatgctgaccgc


gggaagttgcccggcaagaagctgccgctggaggtgctcaaagagatgga


agccaatgcccggaaagctggctgcaccaggggctgtctgatctgcctgt


cccacatcaagtgcacgcccaagatgaagaagttcatcccaggacgctgc


cacacctacgaaggcgacaaagagtccgcacagggcggcataggcgaggc


gatcgtcgacattcctgagattcctgggttcaaggacttggagcccatgg


agcagttcatcgcacaggtcgatctgtgtgtggactgcacaactggctgc


ctcaaagggcttgccaacgtgcagtgttctgacctgctcaagaagtggct


gccgcaacgctgtgcgacctttgccagcaagatccagggccaggtggaca


agatcaagggggccggtggtgacaagcgctcctactccatggagcacttc


cgctggggcaagccggtgggcaagaaacggcgcccggtgaaggtgtaccc


caacgttgctgagaacgagtcggcggaggcctttcccctagagttcaaga


gggagctggaaggcgagcggccattaggcttggagcaggtcctggagtcc


gacgcggagaaggacgacgggccctaccgggtggagcacttccgctggag


caacccgcccaaggacaagcgttacggtggcttcatgacctccgagaaga


gccagacgcccctggtgacgctcttcaagaacgccatcatcaagaacgcg


cacaagaagggccagtga


 


POMC (ACTH, MSH) Human (nucleic acid sequence):


(SEQ ID NO: 156)


Atgccgagatcgtgctgcagccgctcgggggccctgttgctggccttgct


gcttcaggcctccatggaagtgcgtggctggtgcctggagagcagccagt


gtcaggacctcaccacggaaagcaacctgctggagtgcatccgggcctgc


aagcccgacctctcggccgagactcccatgttcccgggaaatggcgacga


gcagcctctgaccgagaacccccggaagtacgtcatgggccacttccgct


gggaccgattcggccgccgcaacagcagcagcagcggcagcagcggcgca


gggcagaagcgcgaggacgtctcagcgggcgaagactgcggcccgctgcc


tgagggcggccccgagccccgcagcgatggtgccaagccgggcccgcgcg


agggcaagcgcaagcccaccgagaacaacgaagacttcaacatcgtggcc


gtggccagcaacttcgcgaccacggatctcgatgctgaccgcgggaagtt


gcccggcaagaagctgccgctggaggtgctcaaagagatggaagccaatg


cccggaaagctggctgcaccaggggctgtctgatctgcctgtcccacatc


aagtgcacgcccaagatgaagaagttcatcccaggacgctgccacaccta


cgaaggcgacaaagagtccgcacagggcggcataggcgaggcgatcgtcg


acattcctgagattcctgggttcaaggacttggagcccatggagcagttc


atcgcacaggtcgatctgtgtgtggactgcacaactggctgcctcaaagg


gcttgccaacgtgcagtgttctgacctgctcaagaagtggctgccgcaac


gctgtgcgacctttgccagcaagatccagggccaggtggacaagatcaag


ggggccggtggtgacaagcgctcctactccatggagcacttccgctgggg


caagccggtgggcaagaagcggcgcccagtgaaggtgtaccctaacggcg


ccgaggacgagtcggccgaggccttccccctggagttcaagagggagctg


actggccagcgactccgggagggagatggccccgacggccctgccgatga


cggcgcaggggcccaggccgacctggagcacagcctgctggtggcggccg


agaagaaggacgagggcccctacaggatggagcacttccgctggggcagc


ccgcccaaggacaagcgctacggcggtttcatgacctccgagaagagcca


gacgcccctggtgacgctgttcaaaaacgccatcatcaagaacgcctaca


agaagggcgagtga


 


Oxytocin Mouse (nucleic acid sequence):


(SEQ ID NO: 157)


Atggcctgccccagtctcgcttgctgcctgcttggcttactggctctgac


ctcggcctgctacatccagaactgccccctgggcggcaagaggaagccca


ccgagaacaacgaagacttcaacatcgtggccgtggccagcaacttcgcg


accacggatctcgatgctgaccgcgggaagttgcccggcaagaagctgcc


gctggaggtgctcaaagagatggaagccaatgcccggaaagctggctgca


ccaggggctgtctgatctgcctgtcccacatcaagtgcacgcccaagatg


aagaagttcatcccaggacgctgccacacctacgaaggcgacaaagagtc


cgcacagggcggcataggcgaggcgatcgtcgacattcctgagattcctg


ggttcaaggacttggagcccatggagcagttcatcgcacaggtcgatctg


tgtgtggactgcacaactggctgcctcaaagggcttgccaacgtgcagtg


ttctgacctgctcaagaagtggctgccgcaacgctgtgcgacctttgcca


gcaagatccagggccaggtggacaagatcaagggggccggtggtgacaag


cgcgctgtgctggacctggatatgcgcaagtgtctcccctgcggcccggg


cggcaaaggacgctgcttcggaccaagcatctgctgcgcggacgagctgg


gctgcttcgtgggcaccgccgaggcgctgcgctgccaggaggagaactac


ctgccttcgccctgccagtctggccagaagccctgcgggagcggaggccg


ctgcgccgccacaggcatctgctgcagcccggatggctgccgcacagacc


ccgcctgcgaccctgagtctgccttctcggagcgctga


 


Oxytocin Human (nucleic acid sequence):


(SEQ ID NO: 158)


Atggccggccccagcctcgcttgctgtctgctcggcctcctggcgctgac


ctccgcctgctacatccagaactgccccctgggaggcaagaggaagccca


ccgagaacaacgaagacttcaacatcgtggccgtggccagcaacttcgcg


accacggatctcgatgctgaccgcgggaagttgcccggcaagaagctgcc


gctggaggtgctcaaagagatggaagccaatgcccggaaagctggctgca


ccaggggctgtctgatctgcctgtcccacatcaagtgcacgcccaagatg


aagaagttcatcccaggacgctgccacacctacgaaggcgacaaagagtc


cgcacagggcggcataggcgaggcgatcgtcgacattcctgagattcctg


ggttcaaggacttggagcccatggagcagttcatcgcacaggtcgatctg


tgtgtggactgcacaactggctgcctcaaagggcttgccaacgtgcagtg


ttctgacctgctcaagaagtggctgccgcaacgctgtgcgacctttgcca


gcaagatccagggccaggtggacaagatcaagggggccggtggtgacaag


cgcgccgcgccggacctcgacgtgcgcaagtgcctcccctgcggccccgg


gggcaaaggccgctgcttcgggcccaatatctgctgcgcggaagagctgg


gctgcttcgtgggcaccgccgaagcgctgcgctgccaggaggagaactac


ctgccgtcgccctgccagtccggccagaaggcgtgcgggagcgggggccg


ctgcgcggtcttgggcctctgctgcagcccggacggctgccacgccgacc


ctgcctgcgacgcggaagccaccttctcccagcgctga


 


Vasopressin-Neurophysin-2 Mouse (nucleic acid


sequence):


(SEQ ID NO: 159)


atgctcgccaggatgctcaacactacgctctccgcttgtttcctgagcct


gctggccttctcctccgcctgctacttccagaactgcccaagaggcggca


agaggaagcccaccgagaacaacgaagacttcaacatcgtggccgtggcc


agcaacttcgcgaccacggatctcgatgctgaccgcgggaagttgcccgg


caagaagctgccgctggaggtgctcaaagagatggaagccaatgcccgga


aagctggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgc


acgcccaagatgaagaagttcatcccaggacgctgccacacctacgaagg


cgacaaagagtccgcacagggcggcataggcgaggcgatcgtcgacattc


ctgagattcctgggttcaaggacttggagcccatggagcagttcatcgca


caggtcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgc


caacgtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtg


cgacctttgccagcaagatccagggccaggtggacaagatcaagggggcc


ggtggtgacaagcgcgccatctctgacatggagctgagacagtgtctccc


ctgcggcccgggcggcaaaggacgctgcttcggaccaagcatctgctgcg


cggacgagctgggctgcttcgtgggcaccgccgaggcgctgcgctgccag


gaggagaactacctgccctcgccctgccagtccggccagaagccctgcgg


gagcgggggccgctgcgccgccgtgggcatctgctgcagcgacgagagct


gcgtggccgagcccgagtgccacgacggttttttccgcctcacccgcgct


cgggagccaagcaacgccacacagctggacggccctgctcgggcgctgct


gctaaggctggtacagctggctgggacacgggagtccgtggattctgcca


agccccgggtctactga


 


Vasopressin-Neurophysin-2 Human (nucleic acid


sequence):


(SEQ ID NO: 160)


Atgcctgacaccatgctgcccgcctgcttcctcggcctactggccttctc


ctccgcgtgctacttccagaactgcccgaggggcggcaagaggaagccca


ccgagaacaacgaagacttcaacatcgtggccgtggccagcaacttcgcg


accacggatctcgatgctgaccgcgggaagttgcccggcaagaagctgcc


gctggaggtgctcaaagagatggaagccaatgcccggaaagctggctgca


ccaggggctgtctgatctgcctgtcccacatcaagtgcacgcccaagatg


aagaagttcatcccaggacgctgccacacctacgaaggcgacaaagagtc


cgcacagggcggcataggcgaggcgatcgtcgacattcctgagattcctg


ggttcaaggacttggagcccatggagcagttcatcgcacaggtcgatctg


tgtgtggactgcacaactggctgcctcaaagggcttgccaacgtgcagtg


ttctgacctgctcaagaagtggctgccgcaacgctgtgcgacctttgcca


gcaagatccagggccaggtggacaagatcaagggggccggtggtgacaag


cgcgccatgtccgacctggagctgagacagtgcctcccctgcggccccgg


gggcaaaggccgctgcttcgggcccagcatctgctgcgcggacgagctgg


gctgcttcgtgggcacggctgaggcgctgcgctgccaggaggagaactac


ctgccgtcgccctgccagtccggccagaaggcgtgcgggagcgggggccg


ctgcgccgccttcggcgtttgctgcaacgacgagagctgcgtgaccgagc


ccgagtgccgcgagggctttcaccgccgcgcccgcgccagcgaccggagc


aacgccacgcagctggacgggccggccggggccttgctgctgcggctggt


gcagctggccggggcgcccgagcccttcgagcccgcccagcccgacgcct


actga


 


Gonadotropin-releasing hormone (GnRH) Mouse


(nucleic acid sequence):


(SEQ ID NO: 161)


Atgatcctcaaactgatggccggcattctactgctgactgtgtgtttgga


aggctgctccagccagcactggtcctatgggttgcgccctgggggaaaga


gaaagcccaccgagaacaacgaagacttcaacatcgtggccgtggccagc


aacttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaa


gaagctgccgctggaggtgctcaaagagatggaagccaatgcccggaaag


ctggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacg


cccaagatgaagaagttcatcccaggacgctgccacacctacgaaggcga


caaagagtccgcacagggcggcataggcgaggcgatcgtcgacattcctg


agattcctgggttcaaggacttggagcccatggagcagttcatcgcacag


gtcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgccaa


cgtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcga


cctttgccagcaagatccagggccaggtggacaagatcaagggggccggt


ggtgacaagcgcaacactgaacacttggttgagtctttccaagagatggg


caaggaggtggatcaaatggcagaaccccagcacttcgaatgtactgtcc


actggccccgttcacccctcagggatctgcgaggagctctggaaagtctg


attgaagaggaagccaggcagaagaagatgtag


 


Gonadotropin-releasing hormone (GnRH) Human


(nucleic acid sequence):


(SEQ ID NO: 162)


Atgaagccaattcaaaaactcctagctggccttattctactgacttggtg


cgtggaaggctgctccagccagcactggtcctatggactgcgccctggag


gaaagagaaagcccaccgagaacaacgaagacttcaacatcgtggccgtg


gccagcaacttcgcgaccacggatctcgatgctgaccgcgggaagttgcc


cggcaagaagctgccgctggaggtgctcaaagagatggaagccaatgccc


ggaaagctggctgcaccaggggctgtctgatctgcctgtcccacatcaag


tgcacgcccaagatgaagaagttcatcccaggacgctgccacacctacga


aggcgacaaagagtccgcacagggcggcataggcgaggcgatcgtcgaca


ttcctgagattcctgggttcaaggacttggagcccatggagcagttcatc


gcacaggtcgatctgtgtgtggactgcacaactggctgcctcaaagggct


tgccaacgtgcagtgttctgacctgctcaagaagtggctgccgcaacgct


gtgcgacctttgccagcaagatccagggccaggtggacaagatcaagggg


gccggtggtgacaagcgcgatgccgaaaatttgattgattctttccaaga


gatagtcaaagaggttggtcaactggcagaaacccaacgcttcgaatgca


ccacgcaccagccacgttctcccctccgagacctgaaaggagctctggaa


agtctgattgaagaggaaactgggcagaagaagatttaa


 


Thyroid-stimulating hormone, beta subunit (TSHB)


Mouse (nucleic acid sequence):


(SEQ ID NO: 163)


Atgagtgctgccgtcctcctctccgtgctttttgctcttgcttgtgggca


agcagcatccttttgtattcccactgagtatacaatgtacgtggatagga


gagagtgtgcctactgcctgaccatcaacaccaccatctgtgctgggtat


tgtatgacacgggatatcaatggcaaactgtttcttcccaaatatgcact


ctctcaggatgtctgtacatacagagacttcatctacagaacggtggaaa


taccaggatgcccgcaccatgttactccttatttctccttccctgtcgcc


ataagctgcaagtgtggcaagtgtaatactgacaacagtgactgcataca


cgaggctgtcagaaccaactactgcaccaagccgcagtctttctatctga


agcccaccgagaacaacgaagacttcaacatcgtggccgtggccagcaac


ttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaagaa


gctgccgctggaggtgctcaaagagatggaagccaatgcccggaaagctg


gctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacgccc


aagatgaagaagttcatcccaggacgctgccacacctacgaaggcgacaa


agagtccgcacagggcggcataggcgaggcgatcgtcgacattcctgaga


ttcctgggttcaaggacttggagcccatggagcagttcatcgcacaggtc


gatctgtgtgtggactgcacaactggctgcctcaaagggcttgccaacgt


gcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcgacct


ttgccagcaagatccagggccaggtggacaagatcaagggggccggtggt


gactatctggggggattttctgtttaa


 


Thyroid-stimulating hormone, beta subunit (TSHB)


Human (nucleic acid sequence):


(SEQ ID NO: 164)


Atgactgctctctttctgatgtccatgctttttggccttacatgtgggca


agcgatgtctttttgtattccaactgagtatacaatgcacatcgaaagga


gagagtgtgcttattgcctaaccatcaacaccaccatctgtgctggatat


tgtatgacacgggatatcaatggcaaactgtttcttcccaaatatgctct


gtcccaggatgtttgcacatatagagacttcatctacaggactgtagaaa


taccaggatgcccactccatgttgctccctatttttcctatcctgttgct


ttaagctgtaagtgtggcaagtgcaatactgactatagtgactgcataca


tgaagccatcaagacaaactactgtaccaaacctcagaagtcttatctga


agcccaccgagaacaacgaagacttcaacatcgtggccgtggccagcaac


ttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaagaa


gctgccgctggaggtgctcaaagagatggaagccaatgcccggaaagctg


gctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacgccc


aagatgaagaagttcatcccaggacgctgccacacctacgaaggcgacaa


agagtccgcacagggcggcataggcgaggcgatcgtcgacattcctgaga


ttcctgggttcaaggacttggagcccatggagcagttcatcgcacaggtc


gatctgtgtgtggactgcacaactggctgcctcaaagggcttgccaacgt


gcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcgacct


ttgccagcaagatccagggccaggtggacaagatcaagggggccggtggt


gactatctggtaggattttctgtctaa


 


Cortisol-releasing factor (CRF) Mouse (nucleic


acid sequence):


(SEQ ID NO: 165)


Atgcggctgcggctgctggtgtccgcgggcatgctgctggtggctctgtc


gtcctgcctgccttgcagggccctgctcagcaggggatccgtcccccgag


cgccgcgggccccgcagcccttgaatttcttgcagccggagcagccccag


caacctcagccggttctgatccgcatgggtgaagaatacttcctccgcct


ggggaatctcaacagaagtcccgctgctcggctgtcccccaactccacgc


ccctcaccgcgggtcgcggcagccgcccctcgcacgaccaggctgcggct


aactttttccgcgtgttgctgcagcagctgcagatgcctcagcgctcgct


cgacagccgcgcggagccggccgaacgcggcgccgaggatgccctcggtg


gccaccagggggcgctggagagggagaggcggaagcccaccgagaacaac


gaagacttcaacatcgtggccgtggccagcaacttcgcgaccacggatct


cgatgctgaccgcgggaagttgcccggcaagaagctgccgctggaggtgc


tcaaagagatggaagccaatgcccggaaagctggctgcaccaggggctgt


ctgatctgcctgtcccacatcaagtgcacgcccaagatgaagaagttcat


cccaggacgctgccacacctacgaaggcgacaaagagtccgcacagggcg


gcataggcgaggcgatcgtcgacattcctgagattcctgggttcaaggac


ttggagcccatggagcagttcatcgcacaggtcgatctgtgtgtggactg


cacaactggctgcctcaaagggcttgccaacgtgcagtgttctgacctgc


tcaagaagtggctgccgcaacgctgtgcgacctttgccagcaagatccag








gccgcccatctctctggatctcaccttccaccttctgcgggaagtcttgg


aaatggcccgggcagagcagttagctcagcaagctcacagcaacaggaaa


ctgatggagattatcgggaaatga


 


Cortisol-releasing factor (CRF) Human (nucleic


acid sequence):


(SEQ ID NO: 166)


Atgcggctgccgctgcttgtgtccgcgggagtcctgctggtggctctcct


gccctgcccgccatgcagggcgctcctgagccgcgggccggtcccgggag


ctcggcaggcgccgcagcaccctcagcccttggatttcttccagccgccg


ccgcagtccgagcagccccagcagccgcaggctcggccggtcctgctccg


catgggagaggagtacttcctccgcctggggaacctcaacaagagcccgg


ccgctcccctttcgcccgcctcctcgctcctcgccggaggcagcggcagc


cgcccttcgccggaacaggcgaccgccaactttttccgcgtgttgctgca


gcagctgctgctgcctcggcgctcgctcgacagccccgcggctctcgcgg


agcgcggcgctaggaatgccctcggcggccaccaggaggcaccggagaga


gaaaggcggaagcccaccgagaacaacgaagacttcaacatcgtggccgt


ggccagcaacttcgcgaccacggatctcgatgctgaccgcgggaagttgc


ccggcaagaagctgccgctggaggtgctcaaagagatggaagccaatgcc


cggaaagctggctgcaccaggggctgtctgatctgcctgtcccacatcaa


gtgcacgcccaagatgaagaagttcatcccaggacgctgccacacctacg


aaggcgacaaagagtccgcacagggcggcataggcgaggcgatcgtcgac


attcctgagattcctgggttcaaggacttggagcccatggagcagttcat


cgcacaggtcgatctgtgtgtggactgcacaactggctgcctcaaagggc


ttgccaacgtgcagtgttctgacctgctcaagaagtggctgccgcaacgc


tgtgcgacctttgccagcaagatccagggccaggtggacaagatcaaggg


ggccggtggtgacaggcggtccgaggagcctcccatctccctggatctca


ccttccacctcctccgggaagtcttggaaatggccagggccgagcagtta


gcacagcaagctcacagcaacaggaaactcatggagattattgggaaata


a


 


Atrial natriuretic peptide (ANP) Mouse (nucleic


acid sequence):


(SEQ ID NO: 167)


Atgggctccttctccatcaccctgggcttcttcctcgtcttggccttttg


gcttccaggccatattggagcaaatcctgtgtacagtgcggtgtccaaca


cagatctgatggatttcaagaacctgctagaccacctggaggagaagatg


ccggtagaagatgaggtcatgcccccgcaggccctgagtgagcagactga


ggaagcaggggccgcacttagctccctccccgaggtgcctccctggactg


gggaggtcaacccacctctgagagacggcagtgctctagggcgcagcccc


tgggacccctccgatagatctgccctcttgaaaagcaaactgagggctct


gctcgctggccctcggagcaagcccaccgagaacaacgaagacttcaaca


tcgtggccgtggccagcaacttcgcgaccacggatctcgatgctgaccgc


gggaagttgcccggcaagaagctgccgctggaggtgctcaaagagatgga


agccaatgcccggaaagctggctgcaccaggggctgtctgatctgcctgt


cccacatcaagtgcacgcccaagatgaagaagttcatcccaggacgctgc


cacacctacgaaggcgacaaagagtccgcacagggcggcataggcgaggc


gatcgtcgacattcctgagattcctgggttcaaggacttggagcccatgg


agcagttcatcgcacaggtcgatctgtgtgtggactgcacaactggctgc


ctcaaagggcttgccaacgtgcagtgttctgacctgctcaagaagtggct


gccgcaacgctgtgcgacctttgccagcaagatccagggccaggtggaca


agatcaagggggccggtggtgaccggagcctacgaagatccagctgcttc


gggggtaggattgacaggattggagcccagagtggactaggctgcaacag


cttccggtaccgaagataa


 


Atrial natriuretic peptide (ANP) Human (nucleic


acid sequence):


(SEQ ID NO: 168)


atgagctccttctccaccaccaccgtgagcttcctccttttactggcatt


ccagctcctaggtcagaccagagctaatcccatgtacaatgccgtgtcca


acgcagacctgatggatttcaagaatttgctggaccatttggaagaaaag


atgcctttagaagatgaggtcgtgcccccacaagtgctcagtgagccgaa


tgaagaagcgggggctgctctcagccccctccctgaggtgcctccctgga


ccggggaagtcagcccagcccagagagatggaggtgccctcgggcggggc


ccctgggactcctctgatcgatctgccctcctaaaaagcaagctgagggc


gctgctcactgcccctcggagcaagcccaccgagaacaacgaagacttca


acatcgtggccgtggccagcaacttcgcgaccacggatctcgatgctgac


cgcgggaagttgcccggcaagaagctgccgctggaggtgctcaaagagat


ggaagccaatgcccggaaagctggctgcaccaggggctgtctgatctgcc


tgtcccacatcaagtgcacgcccaagatgaagaagttcatcccaggacgc


tgccacacctacgaaggcgacaaagagtccgcacagggcggcataggcga


ggcgatcgtcgacattcctgagattcctgggttcaaggacttggagccca


tggagcagttcatcgcacaggtcgatctgtgtgtggactgcacaactggc


tgcctcaaagggcttgccaacgtgcagtgttctgacctgctcaagaagtg


gctgccgcaacgctgtgcgacctttgccagcaagatccagggccaggtgg


acaagatcaagggggccggtggtgaccggagcctgcggagatccagctgc


ttcgggggcaggatggacaggattggagcccagagcggactgggctgtaa


cagcttccggtactga


 


Brain natriuretic peptide (BNP) Mouse (nucleic


acid sequence):


(SEQ ID NO: 169)


atggatctcctgaaggtgctgtcccagatgattctgtttctgcttttcct


ttatctgtcaccgctgggaggtcactcctatcctctgggaagtcctagcc


agtctccagagcaaccaagatgcagaagctgctggagctgataagagaaa


agtcggaggaaatggcccagagacagctcttgaaggaccaaggcctcaca


aaagaacacccaaaaagagtccttcggtctaagcccaccgagaacaacga


agacttcaacatcgtggccgtggccagcaacttcgcgaccacggatctcg


atgctgaccgcgggaagttgcccggcaagaagctgccgctggaggtgctc


aaagagatggaagccaatgcccggaaagctggctgcaccaggggctgtct


gatctgcctgtcccacatcaagtgcacgcccaagatgaagaagttcatcc


caggacgctgccacacctacgaaggcgacaaagagtccgcacagggcggc


ataggcgaggcgatcgtcgacattcctgagattcctgggttcaaggactt


ggagcccatggagcagttcatcgcacaggtcgatctgtgtgtggactgca


caactggctgcctcaaagggcttgccaacgtgcagtgttctgacctgctc


aagaagtggctgccgcaacgctgtgcgacctttgccagcaagatccaggg


ccaggtggacaagatcaagggggccggtggtgaccggtctcaaggcagca


ccctccgggtccagcagagacctcagttgttgtag


 


Brain natriuretic peptide (BNP) Human (nucleic


acid sequence):


(SEQ ID NO: 170)


Atggatccccagacagcaccttcccgggcgctcctgctcctgctcttctt


gcatctggctttcctgggaggtcgttcccacccgctgggcagccccggtt


cagcctcggacttggaaacgtccgggttacaggagcagcgcaaccatttg


cagggcaaactgtcggagctgcaggtggagcagacatccctggagcccct


ccaggagagcccccgtcccacaggtgtctggaagtcccgggaggtagcca


ccgagggcatccgtgggcaccgcaaaatggtcctctacaccctgcgggca


ccacgaagcaagcccaccgagaacaacgaagacttcaacatcgtggccgt


ggccagcaacttcgcgaccacggatctcgatgctgaccgcgggaagttgc


ccggcaagaagctgccgctggaggtgctcaaagagatggaagccaatgcc


cggaaagctggctgcaccaggggctgtctgatctgcctgtcccacatcaa


gtgcacgcccaagatgaagaagttcctcccaggacgctgccacacctacg


aaggcgacaaagagtccgcacagggcggcataggcgcggcgatcgtcgac


attcctgagattcctgggttcaaggacttggagcccatggagcagttcat


cgcacaggtcgatctgtgtgtggactgcacaactggctgcctcaaagggc


ttgccaacgtgcagtgttctgacctgctcaagaagtggccgccgcaacgc


tgtgcgaccttcgccagcaagatccagggccaggtggacaagatcaaggg


ggccggtggtgaccgaagccgaagccccaagatggtgcaagggtctggct


gctttgggaggaagatggaccggatcagctcctccagtggcctgggctgc


aacgtgctgaggcggcattaa


 


Renin Mouse (nucleic acid sequence):


(SEQ ID NO: 171)


Atggacagaaggaggatgcctctctgggcactcttgttgctctggagtcc


ttgcaccttcagtctcccaacacgcaccgctacctttgaacgaatcccgc


tcaagaaaatgccttctgtccgggaaatcctggaggagcggggagtggac


atgaccaggctcagtgctgaatggggcgtattcacaaagaggaagcccac


cgagaacaacgaagacttcaacatcgtggccgtggccagcaacttcgcga


ccacggatctcgatgctgaccgcgggaagttgcccggcaagaagctgccg


ctggaggtgctcaaagagatggaagccaatgcccggaaagctggctgcac


caggggctgtctgatctgcctgtcccacatcaagtgcacgcccaagatga


agaagttcatcccaggacgctgccacacctacgaaggcgacaaagagtcc


gcacagggcggcataggcgaggcgatcgtcgacattcctgagattcctgg


gttcaaggacttggagcccatggagcagttcatcgcacaggtcgatctgt


gtgtggactgcacaactggctgcctcaaagggcttgccaacgtgcagtgt


tctgacctgctcacgaagtggctgccgccacgctgtgcgacctttgccag


caagatccagggccaggtggacaagatcaagggggccggtggtgacaaga


ggccttccttgaccaatcttacctcccccgtggtcctcaccaactacctg


aatacccagtactacggcgagattggcatcggtaccccaccccagacctt


caaagtcatctttgacacgggttcagccaacctctgggtgccctccacca


agtgcagccgcctctaccttgcttgtgggattcacagcctctatgagtcc


tctgactcctccagctacatggagaacgggtccgacttcaccatccacta


cggatcagggagagtcaaaggtttcctcagccaggactcggtgactgtgg


gtggaatcactgtgacacagacctttggagaggtcaccgagctgcccctg


atccctttcatgctggccaagtttgacggtgttctaggcatgggctttcc


cgctcaggccgttggcggggttacccctgtctttgaccacattctctccc


agggggtgctaaaggaggaagtgttctctgtctactacaacaggggttcc


cacctgctggggggcgaggtggtgctaggaggtagcgacccgcagcatta


tcaaggcaattttcactatgtgagcatcagcaagactgactcctggcaga


tcacgatgaagggggtgtctgtggggtcttccaccctgctatgtgaagaa


ggctgtgcggtagtggtggacactggttcatcctttatctcggctcctac


gagctccctgaagttgatcatgcaagccctgggagccaaggagaagagaa


tagaagaatatgttgtgaactgtagccaggtgcccaccctccccgacatt


tcctttgacctgggaggcagggcctacacactcagcagtacggactacgt


gctacagtatcccaacaggagagacaagctgtgcacactggctctccatg


ccatggacatcccaccacccactgggcctgtctgggtcctgggtgccacc


ttcatccgcaagttctatacagagtttgatcggcataacaatcgcattgg


attcgccttggcccgctaa


 


Renin Human (nucleic acid sequence):


(SEQ ID NO: 172)


Atggatggatggagaaggatgcctcgctggggactgctgctgctgctctg


gggctcctgtacctttggtctcccgacagacaccaccacctttaaacgga


agcccaccgagaacaacgaagacttcaacatcgtggccgtggccagcaac


ttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaagaa


gctgccgctggaggtgctcaaagagatggaagccaatgcccggaaagctg


gctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacgccc


aagatgaagaagttcatcccaggacgctgccacacctacgaaggcgacaa


agagtccgcacagggcggcataggcgaggcgatcgtcgacattcctgaga


ttcctgggttcaaggacttggagcccatggagcagttcatcgcacaggtc


gatctgtgtgtggactgcacaactggctgcctcaaagggcttgccaacgt


gcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcgacct


ttgccagcaagatccagggccaggtggacaagatcaagggggccggtggt


gacaaacggatcttcctcaagagaatgccctcaatccgagaaagcctgaa


ggaacgaggtgtggacatggccaggcttggtcccgagtggagccaaccca


tgaagaggctgacacttggcaacaccacctcctccgtgatcctcaccaac


tacatggacacccagtactatggcgagattggcatcggcaccccacccca


gaccttcaaagtcgtctttgacactggttcgtccaatgtttgggtgccct


cctccaagtgcagccgtctctacactgcctgtgtgtatcacaagctcttc


gatgcttcggattcctccagctacaagcacaatggaacagaactcaccct


ccgctattcaacagggacagtcagtggctttctcagccaggacatcatca


ccgtgggtggaatcacggtgacacagatgtttggagaggtcacggagatg


cccgccttacccttcatgctggccgagtttgatggggttgtgggcatggg


cttcattgaacaggccattggcagggtcacccctatcttcgacaacatca


tctcccaaggggtgctaaaagaggacgtcttctctttctactacaacaga


gattccgagaattcccaatcgctgggaggacagattgtgctgggaggcag


cgacccccagcattacgaagggaatttccactatatcaacctcatcaaga


ctggtgtctggcagattcaaatgaagggggtgtctgtggggtcatccacc


ttgctctgtgaagacggctgcctggcattggtagacaccggtgcatccta


catctcaggttctaccagctccatagagaagctcatggaggccttgggag


ccaagaagaggctgtttgattatgtcgtgaagtgtaacgagggccctaca


ctccccgacatctctttccacctgggaggcaaagaatacacgctcaccag


cgcggactatgtatttcaggaatcctacagtagtaaaaagctgtgcacac


tggccatccacgccatggatatcccgccacccactggacccacctgggcc


ctgggggccaccttcatccgaaagttctacacagagtttgatcggcgtaa


caaccgcattggcttcgccttggcccgctga


 


Galanin Mouse (nucleic acid sequence):


(SEQ ID NO: 173)


Atggccagaggcagcgttatcctgctaggctggctcctgttggttgtgac


cctgtcagccactctgggacttgggatgcctgcaaaggagaagagaggta


agcccaccgagaacaacgaagacttcaacatcgtggccgtggccagcaac


ttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaagaa


gctgccgctggaggtgctcaaagagatggaagccaatgcccggaaagctg


gctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacgccc


aagatgaagaagttcatcccaggacgctgccacacctacgaaggcgacaa


agagtccgcacagggcggcataggcgaggcgatcgtcgacattcctgaga


ttcctgggttcaaggacttggagcccatggagcagttcatcgcacaggtc


gatctgtgtgtggactgcacaactggctgcctcaaagggcttgccaacgt


gcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcgacct


ttgccagcaagatccagggccaggtggacaagatcaagggggccggtggt


gacaagagaggttggaccctgaacagcgctggctaccttctgggcccaca


tgccattgacaaccacagatcatttagcgacaagcatggcctcacaggca


agagggagttacaactggaggtggaggaaaggagaccaggaagtgttgat


gtgcccctgcctgagagcaacattgtccgcactataatggagtttctcag


tttcttgcaccttaaagaggccggggccctcgacagcctgcctggcatcc


ccttggccacctcctcagaagacctagagaagtcctga


 


Galanin Human (nucleic acid sequence):


(SEQ ID NO: 174)


Atggcccgaggcagcgccctcctgctcgcctccctcctcctcgccgcggc


cctttctgcctctgcggggctctggtcgccggccaaggaaaaacgaggca


agcccaccgagaacaacgaagacttcaacatcgtggccgtggccagcaac


ttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaagaa


gctgccgctggaggtgctcaaagagatggaagccaatgcccggaaagctg


gctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacgccc


aagatgaagaagttcatcccaggacgctgccacacctacgaaggcgacaa


agagtccgcacagggcggcataggcgaggcgatcgtcgacattcctgaga


ttcctgggttcaaggacttggagcccatggagcagttcatcgcacaggtc


gatctgtgtgtggactgcacaactggctgcctcaaagggcttgccaacgt


gcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcgacct


ttgccagcaagatccagggccaggtggacaagatcaagggggccggtggt


gacaaacgaggctggaccctgaacagcgcgggctacctgctgggcccaca


tgccgttggcaaccacaggtcattcagcgacaagaatggcctcaccagca


agcgggagctgcggcccgaagatgacatgaaaccaggaagctttgacagg


tccatacctgaaaacaatatcatgcgcacaatcattgagtttctgtcttt


cttgcatctcaaagaggccggtgccctcgaccgcctcctggatctccccg


ccgcagcctcctcagaagacatcgagcggtcctga


 


Orexin Mouse (nucleic acid sequence):


(SEQ ID NO: 175)


Atgaactttccttctacaaaggttccctgggccgccgtgacgctgctgct


gctgctactgctgccgccggcgctgctgtcgcttggggtggacgcacagc


ctctgcccgactgctgtcgccagaagacgtgttcctgccgtctctacgaa


ctgttgcacggagctggcaaccacgctgcgggtatcctgactctgggaaa


gcggcggcctggacctccaggcctccagggacggctgcagcgcctccttc


aggccaacggtaaccacgcagctggcatcctgaccatgggccgccgcaag


cccaccgagaacaacgaagacttcaacatcgtggccgtggccagcaactt


cgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaagaagc


tgccgctggaggtgctcaaagagatggaagccaatgcccggaaagctggc


tgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacgcccaa


gatgaagaagttcatcccaggacgctgccacacctacgaaggcgacaaag


agtccgcacagggcggcataggcgaggcgatcgtcgacattcctgagatt


cctgggttcaaggacttggagcccatggagcagttcatcgcacaggtcga


tctgtgtgtggactgcacaactggctgcctcaaagggcttgccaacgtgc


agtgttctgacctgctcaagaagtggctgccgcaacgctgtgcgaccttt


gccagcaagatccagggccaggtggacaagatcaagggggccggtggtga


cggccgccgcgcaggcgcagagctagagccacatccctgctctggtcgcg


gctgtccgaccgtaactaccaccgctttagcaccccggggagggtccgga


gtctga


 


Orexin Human (nucleic acid sequence):


(SEQ ID NO: 176)


Atgaaccttccttccacaaaggtctcctgggccgccgtgacgctactgct


gctgctgctgctgctgccgcccgcgctgttgtcgtccggggcggctgcac


agcccctgcccgactgctgtcgtcaaaagacttgctcttgccgcctctac


gagctgctgcacggcgcgggcaatcacgcggccggcatcctcacgctggg


caagcggaggtccgggcccccgggcctccagggtcggctgcagcgcctcc


tgcaggccagcggcaaccacgccgcgggcatcctgaccatgggccgccgc


aagcccaccgagaacaacgaagacttcaacatcgtggccgtggccagcaa


cttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaaga


agctgccgctggaggtgctcaaagagatggaagccaatgcccggaaagct


ggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacgcc


caagatgaagaagttcatcccaggacgctgccacacctacgaaggcgaca


aagagtccgcacagggcggcataggcgaggcgatcgtcgacattcctgag


attcctgggttcaaggacttggagcccatggagcagttcatcgcacaggt


cgatctgtgtgtggactgcacaactggctgcctcaaagggcttgccaacg


tgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcgacc


tttgccagcaagatccagggccaggtggacaagatcaagggggccggtgg


tgacggccgccgcgcaggcgcagagccagcgccgcgcccctgcctcgggc


gccgctgttccgccccggccgccgcctccgtcgcgcccggaggacagtcc


gggatctga


 


Ghrelin-Obestatin Mouse (nucleic acid sequence):


(SEQ ID NO: 177)


Atgctgtcttcaggcaccatctgcagtttgctgctactcagcatgctctg


gatggacatggccatggcaggctccagcttcctgagcccagagcaccaga


aagcccagcagagaaaggaatccaagaagccaccagctaaactgcagcca


cgagctaagcccaccgagaacaacgaagacttcaacatcgtggccgtggc


cagcaacttcgcgaccacggatctcgatgctgaccgcgggaagttgcccg


gcaagaagctgccgctggaggtgctcaaagagatggaagccaatgcccgg


aaagctggctgcaccaggggctgtctgatctgcctgtcccacatcaagtg


cacgcccaagatgaagaagttcatcccaggacgctgccacacctacgaag


gcgacaaagagtccgcacagggcggcataggcgaggcgatcgtcgacatt


cctgagattcctgggttcaaggacttggagcccatggagcagttcatcgc


acaggtcgatctgtgtgtggactgcacaactggctgcctcaaagggcttg


ccaacgtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgt


gcgacctttgccagcaagatccagggccaggtggacaagatcaagggggc


cggtggtgacccacgagctctggaaggctggctccacccagaggacagag


gacaagcagaagagacagaggaggagctggagatcaggttcaatgctccc


ttcgatgttggcatcaagctgtcaggagctcagtatcagcagcatggccg


ggccctggggaagtttcttcaggatatcctctgggaagaggtcaaagagg


cgccagctgacaagtaa


 


Ghrelin-Obestatin Human (nucleic acid sequence):


(SEQ ID NO: 178)


Atgccctccccagggaccgtctgcagcctcctgctcctcggcatgctctg


gctggacttggccatggcaggctccagcttcctgagccctgaacaccaga


gagtccagagaaaggagtcgaagaagccaccagccaagctgcagccccga


gctaagcccaccgagaacaacgaagacttcaacatcgtggccgtggccag


caacttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggca


agaagctgccgctggaggtgctcaaagagatggaagccaatgcccggaaa


gctggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcac


gcccaagatgaagaagttcatcccaggacgctgccacacctacgaaggcg


acaaagagtccgcacagggcggcataggcgaggcgatcgtcgacattcct


gagattcctgggttcaaggacttggagcccatggagcagttcatcgcaca


ggtcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgcca


acgtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcg


acctttgccagcaagatccagggccaggtggacaagatcaagggggccgg


tggtgacccccgagctctagcaggctggctccgcccggaagatggaggtc


aagcagaaggggcagaggatgaactggaagtccggttcaacgcccccttt


gatgttggaatcaagctgtcaggggttcagtaccagcagcacagccaggc


cctggggaagtttcttcaggacatcctctgggaagaggccaaagaggccc


cagccgacaagtga


 


Cholecystokinin Mouse (nucleic acid sequence):


(SEQ ID NO: 179)


Atgaagagcggcgtatgtctgtgcgtggtgatggcagtcctagctgctgg


cgccctggcgcagccggtagtccctgcagaagctacggaccccgtggagc


agcgggcgcaagaggcgccccgaaggcagctgcgggctaagcccaccgag


aacaacgaagacttcaacatcgtggccgtggccagcaacttcgcgaccac


ggatctcgatgctgaccgcgggaagttgcccggcaagaagctgccgctgg


aggtgctcaaagagatggaagccaatgcccggaaagctggctgcaccagg


ggctgtctgatctgcctgtcccacatcaagtgcacgcccaagatgaagaa


gttcatcccaggacgctgccacacctacgaaggcgacaaagagtccgcac


agggcggcataggcgaggcgatcgtcgacattcctgagattcctgggttc


aaggacttggagcccatggagcagttcatcgcacaggtcgatctgtgtgt


ggactgcacaactggctgcctcaaagggcttgccaacgtgcagtgttctg


acctgctcaagaagtggctgccgcaacgctgtgcgacctttgccagcaag


atccagggccaggtggacaagatcaagggggccggtggtgaccgaaggca


gctgcgggctgtgctccggacggacggcgagccccgagcgcgcctgggcg


cactgctagcgcgatacatccagcaggtccgcaaagctccttctggccgc


atgtccgttcttaagaacctgcagagcctggaccccagccatagaataag


tgaccgggactacatgggctggatggattttggccggcgcagtgccgagg


actacgaatacccatcgtag


 


Cholecystokinin Human (nucleic acid sequence):


(SEQ ID NO: 180)


atgaacagcggcgtgtgcctgtgcgtgctgatggcggtactggcggctgg


cgccctgacgcagccggtgcctcccgcagatcccgcgggctccgggctgc


agcgggcagaggaggcgccccgtaggcagctgagggtaaagcccaccgag


aacaacgaagacttcaacatcgtggccgtggccagcaacttcgcgaccac


ggatctcgatgctgaccgcgggaagttgcccggcaagaagctgccgctgg


aggtgctcaaagagatggaagccaatgcccggaaagctggctgcaccagg


ggctgtctgatctgcctgtcccacatcaagtgcacgcccaagatgaagaa


gttcatcccaggacgctgccacacctacgaaggcgacaaagagtccgcac


agggcggcataggcgaggcgatcgtcgacattcctgagattcctgggttc


aaggacttggagcccatggagcagttcatcgcacaggtcgatctgtgtgt


ggactgcacaactggctgcctcaaagggcttgccaacgtgcagtgttctg


acctgctcaagaagtggctgccgcaacgctgtgcgacctttgccagcaag


atccagggccaggtggacaagatcaagggggccggtggtgaccgtaggca


gctgagggtatcgcagagaacggatggcgagtcccgagcgcacctgggcg


ccctgctggcaagatacatccagcaggcccggaaagctccttctggacga


atgtccatcgttaagaacctgcagaacctggaccccagccacaggataag


tgaccgggactacatgggctggatggattttggccgtcgcagtgccgagg


agtatgagtacccctcctag


 


Gastrin Mouse (nucleic acid sequence):


(SEQ ID NO: 181)


Atgcctcgactgtgtgtgtacatgctggtcttagtgctggctctagctac


cttctcggaagcttcttggaagccccgctcccagctacaggatgcatcat


ctggaccagggaccaatgaggacctggaacagcgccagttcaacaagctg


ggctcagcctctcaccatcgaaggcagctggggccccagggtcctcaaca


cttcatagcagacctgtccaagaagcagaggccacgaatggaggaagaag


aagaggcctacggatggatggactttggccgccgcagtaagcccaccgag


aacaacgaagacttcaacatcgtggccgtggccagcaacttcgcgaccac


ggatctcgatgctgaccgcgggaagttgcccggcaagaagctgccgctgg


aggtgctcaaagagatggaagccaatgcccggaaagctggctgcaccagg


ggctgtctgatctgcctgtcccacatcaagtgcacgcccaagatgaagaa


gttcatcccaggacgctgccacacctacgaaggcgacaaagagtccgcac


agggcggcataggcgaggcgatcgtcgacattcctgagattcctgggttc


aaggacttggagcccatggagcagttcatcgcacaggtcgatctgtgtgt


ggactgcacaactggctgcctcaaagggcttgccaacgtgcagtgttctg


acctgctcaagaagtggctgccgcaacgctgtgcgacctttgccagcaag


atccagggccaggtggacaagatcaagggggccggtggtgaccgccgcag


tgctgaggaagaccagtag


 


Gastrin Human (nucleic acid sequence):


(SEQ ID NO: 182)


Atgcagcgactatgtgtgtatgtgctgatctttgcactggctctggccgc


cttctctgaagcttcttggaagccccgctcccagcagccagatgcaccct


taggtacaggggccaacagggacctggagctaccctggctggagcagcag


ggcccagcctctcatcatcgaaggcagctgggaccccagggtcccccaca


cctcgtggcagacccgtccaagaagcagggaccatggctggaggaagaag


aagaagcctatggatggatggacttcggccgccgcagtaagcccaccgag


aacaacgaagacttcaacatcgtggccgtggccagcaacttcgcgaccac


ggatctcgatgctgaccgcgggaagttgcccggcaagaagctgccgctgg


aggtgctcaaagagatggaagccaatgcccggaaagctggctgcaccagg


ggctgtctgatctgcctgtcccacatcaagtgcacgcccaagatgaagaa


gttcatcccaggacgctgccacacctacgaaggcgacaaagagtccgcac


agggcggcataggcgaggcgatcgtcgacattcctgagattcctgggttc


aaggacttggagcccatggagcagttcatcgcacaggtcgatctgtgtgt


ggactgcacaactggctgcctcaaagggcttgccaacgtgcagtgttctg


acctgctcaagaagtggctgccgcaacgctgtgcgacctttgccagcaag


atccagggccaggtggacaagatcaagggggccggtggtgaccgccgcag


tgctgaggatgagaactaa


 


Protachykinin-1 (Substance P, Neurokinin A,


Neuropeptide K, Neuropeptide gamma) Mouse (nucleic


acid sequence):


(SEQ ID NO: 183)


atgaaaatcctcgtggccgtggcggtcttttttctcgtttccactcaact


gtttgcagaggaaatcgatgccaacgatgatctaaattattggtccgact


ggtccgacagtgaccagatcaaggaggcaatgccggagccctttgagcat


cttctgcagagaatcgcccgaagaaagcccaccgagaacaacgaagactt


caacatcgtggccgtggccagcaacttcgcgaccacggatctcgatgctg


accgcgggaagttgcccggcaagaagctgccgctggaggtgctcaaagag


atggaagccaatgcccggaaagctggctgcaccaggggctgtctgatctg


cctgtcccacatcaagtgcacgcccaagatgaagaagttcatcccaggac


gctgccacacctacgaaggcgacaaagagtccgcacagggcggcataggc


gaggcgatcgtcgacattcctgagattcctgggttcaaggacttggagcc


catggagcagttcatcgcacaggtcgatctgtgtgtggactgcacaactg


gctgcctcaaagggcttgccaacgtgcagtgttctgacctgctcaagaag


tggctgccgcaacgctgtgcgacctttgccagcaagatccagggccaggt


ggacaagatcaagggggccggtggtgaccgaagacccaagcctcagcagt


tctttggattaatgggcaagcgggatgctgattcctcagttgaaaaacaa


gtggccctgttaaaggctctttatggacatggccagatctctcacaaaag


gcataaaacagattcctttgttggactaatgggcaaaagagctttaaatt


ctgtggcttatgaaagaagcgcgatgcagaactacgaaagaagacgtaaa


taa


 


Protachykinin-1 (Substance P, Neurokinin A,


Neuropeptide K, Neuropeptide gamma) Human (nucleic


acid sequence):


(SEQ ID NO: 184)


atgaaaatcctcgtggccttggcagtcttttttcttgtctccactcagct


gtttgcagaagaaataggagccaatgatgatctgaattactggtccgact


ggtacgacagcgaccagatcaaggaggaactgccggagccctttgagcat


cttctgcagagaatcgcccggagaaagcccaccgagaacaacgaagactt


caacatcgtggccgtggccagcaacttcgcgaccacggatctcgatgctg


accgcgggaagttgcccggcaagaagctgccgctggaggtgctcaaagag


atggaagccaatgcccggaaagctggctgcaccaggggctgtctgatctg


cctgtcccacatcaagtgcacgcccaagatgaagaagttcatcccaggac


gctgccacacctacgaaggcgacaaagagtccgcacagggcggcataggc


gaggcgatcgtcgacattcctgagattcctgggttcaaggacttggagcc


catggagcagttcatcgcacaggtcgatctgtgtgtggactgcacaactg


gctgcctcaaagggcttgccaacgtgcagtgttctgacctgctcaagaag


tggctgccgcaacgctgtgcgacctttgccagcaagatccagggccaggt


ggacaagatcaagggggccggtggtgaccggagacccaagcctcagcagt


tctttggattaatgggcaaacgggatgctgattcctcaattgaaaaacaa


gtggccctgttaaaggctctttatggacatggccagatctctcacaaaag


acataaaacagattcctttgttggactaatgggcaaaagagctttaaatt


ctgtggcttatgaaaggagtgcaatgcagaattatgaaagaagacgttaa


 


Proenkephalin-A Mouse (nucleic acid sequence):


(SEQ ID NO: 185)


atggcgcggttcctgaggctttgcacctggctgctggcgcttgggtcctg


cctcctggctacagtgcaggcggaatgcagccaggactgcgctaaatgca


gctaccgcctggttcgcccaggcgacatcaatttcctggcgtgcacactg


gaatgtgaaggacagctgccttctttcaaaatctgggagacctgcaagga


tctcctgcaggtgtccaggcccgagttcccttgggataacatcgacatgt


acaaagacagcagcaaacaggatgagagccacttgctagccaagaagtac


ggaggcttcatgaaacggtacggaggcttcatgaagaagatggacgagct


atatcccatggagccagaagaagaagcgaacggaggagagatccttgcca


agaggtatggcggcttcatgaagaaggatgcagatgagggagacaccttg


gccaactcctccgatctgctgaaagagctactgggaacgggagacaaccg


tgcgaaagacagccaccaacaagagagcaccaacaatgacgaagacatga


gcaagaggtatgggggcttcatgagaagcctcaaaagaagcccccaactg


gaagatgaagcaaaagagctgcagaagcgctacgggggcttcatgagaag


gaagcccaccgagaacaacgaagacttcaacatcgtggccgtggccagca


acttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaag


aagctgccgctggaggtgctcaaagagatggaagccaatgcccggaaagc


tggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacgc


ccaagatgaagaagttcatcccaggacgctgccacacctacgaaggcgac


aaagagtccgcacagggcggcataggcgaggcgatcgtcgacattcctga


gattcctgggttcaaggacttggagcccatggagcagttcatcgcacagg


tcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgccaac


gtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcgac


ctttgccagcaagatccagggccaggtggacaagatcaagggggccggtg


gtgacagaagggtgggacgccccgagtggtggatggactaccagaagagg


tatgggggcttcctgaagcgctttgctgagtctctgccctccgatgaaga


aggcgaaaattactcgaaagaagttcctgagatagagaaaagatacgggg


gctttatgcggttctga


 


Proenkephalin-A Human (nucleic acid sequence):


(SEQ ID NO: 186)


atggcgcggttcctgacactttgcacttggctgctgttgctcggccccgg


gctcctggcgaccgtgcgggccgaatgcagccaggattgcgcgacgtgca


gctaccgcctagtgcgcccggccgacatcaacttcctggcttgcgtaatg


gaatgtgaaggtaaactgccttctctgaaaatttgggaaacctgcaagga


gctcctgcagctgtccaaaccagagcttcctcaagatggcaccagcaccc


tcagagaaaatagcaaaccggaagaaagccatttgctagccaaaaggtat


gggggcttcatgaaaaggtatggaggcttcatgaagaaaatggatgagct


ttatcccatggagccagaagaagaggccaatggaagtgagatcctcgcca


agcggtatgggggcttcatgaagaaggatgcagaggaggacgactcgctg


gccaattcctcagacctgctaaaagagcttctggaaacaggggacaaccg


agagcgtagccaccaccaggatggcagtgataatgaggaagaagtgagca


agagatatgggggcttcatgagaggcttaaagagaagcccccaactggaa


gatgaagccaaagagctgcagaagcgatatgggggcttcatgagaagaaa


gcccaccgagaacaacgaagacttcaacatcgtggccgtggccagcaact


tcgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaagaag


ctgccgctggaggtgctcaaagagatggaagccaatgcccggaaagctgg


ctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacgccca


agatgaagaagttcatcccaggacgctgccacacctacgaaggcgacaaa


gagtccgcacagggcggcataggcgaggcgatcgtcgacattcctgagat


tcctgggttcaaggacttggagcccatggagcagttcatcgcacaggtcg


atctgtgtgtggactgcacaactggctgcctcaaagggcttgccaacgtg


cagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcgacctt


tgccagcaagatccagggccaggtggacaagatcaagggggccggtggtg


acagaagagtaggtcgcccagagtggtggatggactaccagaaacggtat


ggaggtttcctgaagcgctttgccgaggctctgccctccgacgaagaagg


cgaaagttactccaaagaagttcctgaaatggaaaaaagatacggaggat


ttatgagattttaa


 


Proenkephalin-B Mouse (nucleic acid sequence):


(SEQ ID NO: 187)


atggcgtggtccaggctgatgctggcagcttgcctcctcgtgatgccctc


taatgttatggcggactgcctgtccctgtgctccctgtgtgcagtgagga


ttcaggatgggccccgtcccatcaaccccctgatttgctccctggagtgc


caggacctggtgccgccctcagaggagtgggagacatgccggggcttctc


atcttttctcaccctgacggtctctgggctccgtggcaaggatgacttgg


aagatgaggttgctttggaagaaggctacagtgcactagccaagctcttg


gaacccgtcctgaaggagctggagaaaagccgactccttaccagcgtccc


agaggaaaagttcaggggtctctccagcagctttggcaacggaaaagaat


ctgagctggcgggtgctgaccggatgaatgatgaagccgcacaggcgggc


acgctccattttaatgaggaggacttgagaaaacaggccaaacgctatgg


cggctttttgcgcaaataccccaagaggaagcccaccgagaacaacgaag


acttcaacatcgtggccgtggccagcaacttcgcgaccacggatctcgat


gctgaccgcgggaagttgcccggcaagaagctgccgctggaggtgctcaa


agagatggaagccaatgcccggaaagctggctgcaccaggggctgtctga


tctgcctgtcccacatcaagtgcacgcccaagatgaagaagttcatccca


ggacgctgccacacctacgaaggcgacaaagagtccgcacagggcggcat


aggcgaggcgatcgtcgacattcctgagattcctgggttcaaggacttgg


agcccatggagcagttcatcgcacaggtcgatctgtgtgtggactgcaca


actggctgcctcaaagggcttgccaacgtgcagtgttctgacctgctcaa


gaagtggctgccgcaacgctgtgcgacctttgccagcaagatccagggcc


aggtggacaagatcaagggggccggtggtgacaagaggagttccgagatg


gcccgggatgaggacgggggccaggatggggatcaggtagggcatgagga


cctgtacaaacgctatgggggcttcctgcggcgcattcgccccaagctga


agtgggacaaccagaagcgctatggtggtttcctgcggcgtcagttcaag


gtggtgacgcggtcccaggagaaccccaatacctattctgaagatttaga


tgtttga


 


Proenkephalin-B Human (nucleic acid sequence):


(SEQ ID NO: 188)


atggcctggcaggggctggtcctggctgcctgcctcctcatgttcccctc


caccacagcggactgcctgtcgcggtgctccttgtgtgctgtaaagaccc


aggatggtcccaaacctatcaatcccctgatttgctccctgcaatgccag


gctgccctgctgccctctgaggaatgggagagatgccagagctttctgtc


ttttttcaccccctccacccttgggctcaatgacaaggaggacttgggga


gcaagtcggttggggaagggccctacagtgagctggccaagctctctggg


tcattcctgaaggagctggagaaaagcaagtttctcccaagtatctcaac


aaaggagaacactctgagcaagagcctggaggagaagctcaggggtctct


ctgacgggtttagggagggagcagagtctgagctgatgagggatgcccag


ctgaacgatggtgccatggagactggcacactctatctcgctgaggagga


ccccaaggagcaggtcaaacgcaagcccaccgagaacaacgaagacttca


acatcgtggccgtggccagcaacttcgcgaccacggatctcgatgctgac


cgcgggaagttgcccggcaagaagctgccgctggaggtgctcaaagagat


ggaagccaatgcccggaaagctggctgcaccaggggctgtctgatctgcc


tgtcccacatcaagtgcacgcccaagatgaagaagttcatcccaggacgc


tgccacacctacgaaggcgacaaagagtccgcacagggcggcataggcga


ggcgatcgtcgacattcctgagattcctgggttcaaggacttggagccca


tggagcagttcatcgcacaggtcgatctgtgtgtggactgcacaactggc


tgcctcaaagggcttgccaacgtgcagtgttctgacctgctcaagaagtg


gctgccgcaacgctgtgcgacctttgccagcaagatccagggccaggtgg


acaagatcaagggggccggtggtgacaaacgctatgggggctttttgcgc


aaataccccaagaggagctcagaggtggctggggagggggacggggatag


catgggccatgaggacctgtacaaacgctatgggggcttcttgcggcgca


ttcgtcccaagctcaagtgggacaaccagaagcgctatggcggttttctc


cggcgccagttcaaggtggtgactcggtctcaggaagatccgaatgctta


ctctggagagctttttgatgcataa


 


Insulin-like growth hormone 1 (IGF-1) Mouse


(nucleic acid sequence):


(SEQ ID NO: 189)


atggggaaaatcagcagccttccaactcaattatttaagatctgcctctg


tgacttcttgaagataaagatacacatcatgtcgtcttcacacctcttct


acctggcgctctgcttgctcaccttcaccagctccaccacagctggacca


gagaccctttgcggggctgagctggtggatgctcttcagttcgtgtgtgg


accgaggggcttttacttcaacaagcccacaggctatggctccagcattc


ggaggaagcccaccgagaacaacgaagacttcaacatcgtggccgtggcc


agcaacttcgcgaccacggatctcgatgctgaccgcgggaagttgcccgg


caagaagctgccgctggaggtgctcaaagagatggaagccaatgcccgga


aagctggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgc


acgcccaagatgaagaagttcatcccaggacgctgccacacctacgaagg


cgacaaagagtccgcacagggcggcataggcgaggcgatcgtcgacattc


ctgagattcctgggttcaaggacttggagcccatggagcagttcatcgca


caggtcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgc


caacgtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtg


cgacctttgccagcaagatccagggccaggtggacaagatcaagggggcc


ggtggtgactccagcattcggagggcacctcagacaggcattgtggatga


gtgttgcttccggagctgtgatctgaggagactggagatgtactgtgccc


cactgaagcctacaaaagcagcccgctctatccgtgcccagcgccacact


gacatgcccaagactcagaagtccccgtccctatcgacaaacaagaaaac


gaagctgcaaaggagaaggaaaggaagtacatttgaagaacacaagtag


 


Insulin-like growth hormone 1 (IGF-1) Human


(nucleic acid sequence):


(SEQ ID NO: 190)


atgggaaaaatcagcagtcttccaacccaattatttaagtgctgcttttg


tgatttcttgaaggtgaagatgcacaccatgtctcctcgcatctcttcta


cctggcgctgtgcctgctcaccttcaccagctctgccacggctggaccgg


agacgctctgcggggctgagctggtggatgctcttcagttcgtgtgtgga


gacaggggcttttatttcaacaagcccacagggtatggctccagcagtcg


gaggaagcccaccgagaacaacgaagacttcaacatcgtggccgtggcca


gcaacttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggc


aagaagctgccgctggaggtgctcaaagagatggaagccaatgcccggaa


agctggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgca


cgcccaagatgaagaagttcatcccaggacgctgccacacctacgaaggc


gacaaagagtccgcacagggcggcataggcgaggcgatcgtcgacattcc


tgagattcctgggttcaaggacttggagcccatggagcagttcatcgcac


aggtcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgcc


aacgtgcagtgttctgatctgctcaagaagtggctgccgcaacgctgtgc


gacctttgccagcaagatccagggccaggtggacaagatcaagggggccg


gtggtgacagcagtcggagggcgcctcagacaggcatcgtggatgagtgc


tgcttccggagctgtgatctaaggaggctggagatgtattgcgcacccct


caagcctgccaagtcagctcgctctgtccgtgcccagcgccacaccgaca


tgcccaagacccagaagtatcagcccccatctaccaacaagaacacgaag


tctcagagaaggaaaggaagtacatttgaagaacgcaagtag


 


Insulin-like growth hormone 2 (IGF-2) Mouse


(nucleic acid sequence):


(SEQ ID NO: 191)


atgggcggcagcgtcgccggcttccaggtaccaatggggatcccagtggg


gaagtcgatgttggtgcttctcatctctttggccttcgccttgtgctgca


tcgctgcttacggccccggagagactctgtgcggaggggagcttgttgac


acgcttcagtttgtctgttcggaccgcggcttctacttcagcaggccttc


aagccgtgccaaccgtcgcagccgtggcatcgtggaagagtgctgcttcc


gcagctgcgacctggccctcctggagacatactgtgccacccccgccaag


tccgagagggacaagcccaccgagaacaacgaagacttcaacatcgtggc


cgtggccagcaacttcgcgaccacggatctcgatgctgaccgcgggaagt


tgcccggcaagaagctgccgctggaggtgctcaaagagatggaagccaat


gcccggaaagctggctgcaccaggggctgtctgatctgcctgtcccacat


caagtgcacgcccaagatgaagaagttcatcccaggacgctgccacacct


acgaaggcgacaaagagtccgcacagggcggcataggcgaggcgatcgtc


gacattcctgagattcctgggttcaaggacttggagcccatggagcagtt


catcgcacaggtcgatctgtgtgtggactgcacaactggctgcctcaaag


ggcttgccaacgtgcagtgttctgacctgctcaagaagtggctgccgcaa


cgctgtgcgacctttgccagcaagatccagggccaggtggacaagatcaa


gggggccggtggtgacgagagggacgtgtctacctctcaggccgtacttc


cggacgacttccccagataccccgtgggcaagttcttccaatatgacacc


tggagacagtccgcgggacgcctgcgcagaggcctgcctgccctcctgcg


tgcccgccggggtcgcatgcttgccaaagagctcaaagagttcagagagg


ccaaacgtcatcgtcccctgatcgtgttaccacccaaagaccccgcccac


gggggagcctcttcggagatgtccagcaaccatcagtga


 


Insulin-like growth hormone 2 (IGF-2) Human


(nucleic acid sequence):


(SEQ ID NO: 192)


atgggaatcccaatggggaagtcgatgctggtgcttctcaccttcttggc


cttcgcctcgtgctgcattgctgcttaccgccccagtgagaccctgtgcg


gcggggagctggtggacaccctccagttcgtctgtggggaccgcggcttc


tacttcagcaggcccgcaagccgtgtgagccgtcgcagccgtggcatcgt


tgaggagtgctgtttccgcagctgtgacctggccctcctggagacgtact


gtgctacccccgccaagtccgagagggacaagcccaccgagaacaacgaa


gacttcaacatcgtggccgtggccagcaacttcgcgaccacggatctcga


tgctgaccgcgggaagttgcccggcaagaagctgccgctggaggtgctca


aagagatggaagccaatgcccggaaagctggctgcaccaggggctgtctg


atctgcctgtcccacatcaagtgcacgcccaagatgaagaagttcatccc


aggacgctgccacacctacgaaggcgacaaagagtccgcacagggcggca


taggcgaggcgatcgtcgacattcctgagattcctgggttcaaggacttg


gagcccatggagcagttcatcgcacaggtcgatctgtgtgtggactgcac


aactggctgcctcaaagggcttgccaacgtgcagtgttctgacctgctca


agaagtggctgccgcaacgctgtgcgacctttgccagcaagatccagggc


caggtggacaagatcaagggggccggtggtgacgagagggacgtgtcgac


ccctccgaccgtgcttccggacaacttccccagataccccgtgggcaagt


tcttccaatatgacacctggaagcagtccacccagcgcctgcgcaggggc


ctgcctgccctcctgcgtgcccgccggggtcacgtgctcgccaaggagct


cgaggcgttcagggaggccaaacgtcaccgtcccctgattgctctaccca


cccaagaccccgcccacgggggcgcccccccagagatggccagcaatcgg


aagtga


 


Parathyroid hormone (PTH) Mouse (nucleic acid


sequence):


(SEQ ID NO: 193)


atgatgtctgcaaacaccgtggctaaagtgatgatcatcatgctggcagt


ctgtcttcttacccaaacggatgggaaacccgtgaggaagagaaagccca


ccgagaacaacgaagacttcaacatcgtggccgtggccagcaacttcgcg


accacggatctcgatgctgaccgcgggaagttgcccggcaagaagctgcc


gctggaggtgctcaaagagatggaagccaatgcccggaaagctggctgca


ccaggggctgtctgatctgcctgtcccacatcaagtgcacgcccaagatg


aagaagttcatcccaggacgctgccacacctacgaaggcgacaaagagtc


cgcacagggcggcataggcgaggcgatcgtcgacattcctgagattcctg


ggttcaaggacttggagcccatggagcagttcatcgcacaggtcgatctg


tgtgtggactgcacaactggctgcctcaaagggcttgccaacgtgcagtg


ttctgacctgctcaagaagtggctgccgcaacgctgtgcgacctttgcca


gcaagatccagggccaggtggacaagatcaagggggccggtggtgacaag


agagctgtcagtgaaatacagcttatgcacaacctgggcaaacacctggc


ctccatggagaggatgcaatggctgagaaggaagctgcaagatatgcaca


attttgttagtcttggagtccaaatggctgccagagatggcagtcaccag


aagcccaccaagaaggaggaaaatgtccttgttgatggcaatccaaaaag


tcttggtgagggagacaaagctgatgtggatgtattagttaaatcaaaat


ctcagtaa


 


Parathyroid hormone (PTH) Human (nucleic acid


sequence):


(SEQ ID NO: 194)


atgatacctgcaaaagacatggctaaagttatgattgtcatgttggcaat


ttgttttcttacaaaatcggatgggaaatctgttaagaagagaaagccca


ccgagaacaacgaagacttcaacatcgtggccgtggccagcaacttcgcg


accacggatctcgatgctgaccgcgggaagttgcccggcaagaagctgcc


gctggaggtgctcaaagagatggaagccaatgcccggaaagctggctgca


ccaggggctgtctgatctgcctgtcccacatcaagtgcacgcccaagatg


aagaagttcatcccaggacgctgccacacctacgaaggcgacaaagagtc


cgcacagggcggcataggcgaggcgatcgtcgacattcctgagattcctg


ggttcaaggacttggagcccatggagcagttcatcgcacaggtcgatctg


tgtgtggactgcacaactggctgcctcaaagggcttgccaacgtgcagtg


ttctgacctgctcaagaagtggctgccgcaacgctgtgcgacctttgcca


gcaagatccagggccaggtggacaagatcaagggggccggtggtgacaag


agatctgtgagtgaaatacagcttatgcataacctgggaaaacatctgaa


ctcgatggagagagtagaatggctgcgtaagaagctgcaggatgtgcaca


attttgttgcccttggagctcctctagctcccagagatgctggttcccag


aggccccgaaaaaaggaagacaatgtcttggttgagagccatgaaaaaag


tcttggagaggcagacaaagctgatgtgaatgtattaactaaagctaaat


cccagtga


 


Parathyroid hormone-related protein (FTHrP) Mouse


(nucleic acid sequence):


(SEQ ID NO: 195)


atgctgcggaggctggttcagcagtggagtgtcctggtattcctgctcag


ctactccgtgccctcccgcgggcgttcggtggaggggcttggccgcaggc


tcaaacgcaagcccaccgagaacaacgaagacttcaacatcgtggccgtg


gccagcaacttcgcgaccacggatctcgatgctgaccgcgggaagttgcc


cggcaagaagctgccgctggaggtgctcaaagagatggaagccaatgccc


ggaaagctggctgcaccaggggctgtctgatctgcctgtcccacatcaag


tgcacgcccaagatgaagaagttcatcccaggacgctgccacacctacga


aggcgacaaagagtccgcacagggcggcataggcgaggcgatcgtcgaca


ttcctgagattcctgggttcaaggacttggagcccatggagcagttcatc


gcacaggtcgatctgtgtgtggactgcacaactggctgcctcaaagggct


tgccaacgtgcagtgttctgacctgctcaagaagtggctgccgcaacgct


gtgcgacctttgccagcaagatccagggccaggtggacaagatcaagggg


gccggtggtgacaaacgcgctgtgtctgaacatcagctactgcatgacaa


gggcaagtccatccaagacttgcgccgccgtttcttcctccaccatctga


tcgcggagatccacacagccgaaatcagagctacctcggaggtgtccccc


aactccaaacctgctcccaacaccaaaaaccaccccgtgcggtttgggtc


agacgatgagggcagatacctaactcaggaaaccaacaaggtggagacgt


acaaagaacagccactcaagacacccgggaagaagaagaaaggcaagcct


gggaaacgcagagaacaggagaaaaagaagcgaaggactcggtctgcctg


gccaagcacagctgcgagtggcctgcttgaggaccccctgccccacacct


ccaggccctcgctggagcccagcttaaggacgcattga


 


Parathyroid hormone-related protein (FTHrP) Human


(nucleic acid sequence):


(SEQ ID NO: 196)


atgcagcggagactggttcagcagtggagcgtcgcggtgttcctgctgag


ctacgcggtgccctcctgcgggcgctcggtggagggtctcagccgccgcc


tcaaaagaaagcccaccgagaacaacgaagacttcaacatcgtggccgtg


gccagcaacttcgcgaccacggatctcgatgctgaccgcgggaagttgcc


cggcaagaagctgccgctggaggtgctcaaagagatggaagccaatgccc


ggaaagctggctgcaccaggggctgtctgatctgcctgtcccacatcaag


tgcacgcccaagatgaagaagttcatcccaggacgctgccacacctacga


aggcgacaaagagtccgcacagggcggcataggcgaggcgatcgtcgaca


ttcctgagattcctgggttcaaggacttggagcccatggagcagttcatc


gcacaggtcgatctgtgtgtggactgcacaactggctgcctcaaagggct


tgccaacgtgcagtgttctgacctgctcaagaagtggctgccgcaacgct


gtgcgacctttgccagcaagatccagggccaggtggacaagatcaagggg


gccggtggtgacaaaagagctgtgtctgaacatcagctcctccatgacaa


ggggaagtccatccaagatttacggcgacgattcttccttcaccatctga


tcgcagaaatccacacagctgaaatcagagctacctcggaggtgtcccct


aactccaagccctctcccaacacaaagaaccaccccgtccgatttgggtc


tgatgatgagggcagatacctaactcaggaaactaacaaggtggagacgt


acaaagagcagccgctcaagacacctgggaagaaaaagaaaggcaagccc


gggaaacgcaaggagcaggaaaagaaaaaacggcgaactcgctctgcctg


gttagactctggagtgactgggagtgggctagaaggggaccacctgtctg


acacctccacaacgtcgctggagctcgattcacggaggcattga


 


Osteocalcin Mouse (nucleic acid sequence):


(SEQ ID NO: 197)


atgaggaccatctttctgctcactctgctgaccctggctgcgctctgtct


ctctgacctcacagatgccaagcccagcggccctgagtctgacaaagcct


tcatgtccaagcaggagggcaataaggtagtgaacagactccggcgcaag


cccaccgagaacaacgaagacttcaacatcgtggccgtggccagcaactt


cgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaagaagc


tgccgctggaggtgctcaaagagatggaagccaatgcccggaaagctggc


tgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacgcccaa


gatgaagaagttcatcccaggacgctgccacacctacgaaggcgacaaag


agtccgcacagggcggcataggcgaggcgatcgtcgacattcctgagatt


cctgggttcaaggacttggagcccatggagcagttcatcgcacaggtcga


tctgtgtgtggactgcacaactggctgcctcaaagggcttgccaacgtgc


agtgttctgacctgctcaagaagtggctgccgcaacgctgtgcgaccttt


gccagcaagatccagggccaggtggacaagatcaagggggccggtggtga


ccggcgctaccttggagcctcagtccccagcccagatcccctggagccca


cccgggagcagtgtgagcttaaccctgcttgtgacgagctatcagaccag


tatggcttgaagaccgcctacaaacgcatctatggtatcactatttag


 


Osteocalcin Human (nucleic acid sequence):


(SEQ ID NO: 198)


atgagagccctcacactcctcgccctattggccctggccgcactttgcat


cgctggccaggcaggtgcgaagcccagcggtgcagagtccagcaaaggtg


cagcctttgtgtccaagcaggagggcagcgaggtagtgaagagacccagg


cgcaagcccaccgagaacaacgaagacttcaacatcgtggccgtggccag


caacttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggca


agaagctgccgctggaggtgctcaaagagatggaagccaatgcccggaaa


gctggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcac


gcccaagatgaagaagttcatcccaggacgctgccacacctacgaaggcg


acaaagagtccgcacagggcggcataggcgaggcgatcgtcgacattcct


gagattcctgggttcaaggacttggagcccatggagcagttcatcgcaca


ggtcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgcca


acgtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcg


acctttgccagcaagatccagggccaggtggacaagatcaagggggccgg


tggtgacaggcgctacctgtatcaatggctgggagccccagtcccctacc


cggatcccctggagcccaggagggaggtgtgtgagctcaatccggactgt


gacgagttggctgaccacatcggctttcaggaggcctatcggcgcttcta


cggcccggtctag


 


Urocortin-3 Mouse (nucleic acid sequence):


(SEQ ID NO: 199)


atgctgatgcccacctacttcctgctgccacttctgctgctcctaggagg


tccaaggacaagcctctcccacaagttctacaacactggaccagtcttca


gctgcctcaacacagccctatctgaggtcaagaagaacaagctggaagat


gtgcccttgctgagcaagaagagctttggccacctgcccacacaagaccc


ctcaggggaagaagatgacaaccaaacgcacctccagatcaaaagaactt


tctcaggtgccgcgggtgggaatggagctgggagcacccggtacagatac


caatcccaggcacagcacaaggggaagctgtacccagacaagcccaaaag


cgaccggggcaccaagaagcccaccgagaacaacgaagacttcaacatcg


tggccgtggccagcaacttcgcgaccacggatctcgatgctgaccgcggg


aagttgcccggcaagaagctgccgctggaggtgctcaaagagatggaagc


caatgcccggaaagctggctgcaccaggggctgtctgatctgcctgtccc


acatcaagtgcacgcccaagatgaagaagttcatcccaggacgctgccac


acctacgaaggcgacaaagagtccgcacagggcggcataggcgaggcgat


cgtcgacattcctgagattcctgggttcaaggacttggagcccatggagc


agttcatcgcacaggtcgatctgtgtgtggactgcacaactggctgcctc


aaagggcttgccaacgtgcagtgttctgacctgctcaagaagtggctgcc


gcaacgctgtgcgacctttgccagcaagatccagggccaggtggacaaga


tcaagggggccggtggtgaccggggcaccaagttcaccctttcccttgat


gttcccactaacatcatgaacatcctcttcaacatcgacaaggccaagaa


tttgcgagccaaggcagctgccaatgctcagctcatggcacagattggga


agaagaagtaa


 


Urocortin-3 Human (nucleic acid sequence):


(SEQ ID NO: 200)


Atgctgatgccggtccacttcctgctgctcctgctgctgctcctgggggg


ccccaggacaggcctcccccacaagttctacaaagccaagcccatcttca


gctgcctcaacaccgccctgtctgaggctgagaagggccagtgggaggat


gcatccctgctgagcaagaggagcttccactacctgcgcagcagagacgc


ctcttcgggagaggaggaggagggcaaagagaaaaagactttccccatct


ctggggccaggggtggagccagaggcacccggtacagatacgtgtcccaa


gcacagcccaggggaaagccacgccaggacacggccaagagtccccaccg


caagcccaccgagaacaacgaagacttcaacatcgtggccgtggccagca


acttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaag


aagctgccgctggaggtgctcaaagagatggaagccaatgcccggaaagc


tggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacgc


ccaagatgaagaagttcatcccaggacgctgccacacctacgaaggcgac


aaagagtccgcacagggcggcataggcgaggcgatcgtcgacattcctga


gattcctgggttcaaggacttggagcccatggagcagttcatcgcacagg


tcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgccaac


gtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcgac


ctttgccagcaagatccagggccaggtggacaagatcaagggggccggtg


gtgaccaccgcaccaagttcaccctgtccctcgacgtccccaccaacatc


atgaacctcctcttcaacatcgccaaggccaagaacctgcgtgcccaggc


ggccgccaatgcccacctgatggcgcaaattgggaggaagaagtag


 


Urocortin-2 Mouse (nucleic acid sequence):


(SEQ ID NO: 201)


Atgatgaccaggtgggcactggtggtgttcgtggtcctgatgttggatag


gatcctatttgtcccaggaactcctatccccaccttccagctcctccctc


agaactctctggagacaactcctagctctgtgacctcagagagctcctca


ggtaccaccacaggaccctcagcttcctggagcaactctaaagccagccc


ttacctagacacccgtgtcaagcccaccgagaacaacgaagacttcaaca


tcgtggccgtggccagcaacttcgcgaccacggatctcgatgctgaccgc


gggaagttgcccggcaagaagctgccgctggaggtgctcaaagagatgga


agccaatgcccggaaagctggctgcaccaggggctgtctgatctgcctgt


cccacatcaagtgcacgcccaagatgaagaagttcatcccaggacgctgc


cacacctacgaaggcgacaaagagtccgcacagggcggcataggcgaggc


gatcgtcgacattcctgagattcctgggttcaaggacttggagcccatgg


agcagttcatcgcacttggtcgatctgtgtgtggactgcacaactggctg


cctcaaagggcttgccaacgtgcagtgttctgacctgctcaagaagtggc


tgccgcaacgctgtgcgacctttgccagcaagatccagggccaggtggac


aagatcaagggggccggtggtgacacccgtgtcatactctccctggatgt


tcccattggcctcctacggatcttactggaacaggctcgttacaaggctg


ccaggaatcaggctgccactaatgctcaaatactagcccatgttggccgc


cgctga


 


Urocortin-2 Human (nucleic acid sequence):


(SEQ ID NO: 202)


atgaccaggtgtgctctgctgttgctgatggtcctgatgttgggcagagt


cctggttgtcccagtgacccctatcccaaccttccagctccgccctcaga


attctccccagaccactccccgacctgcggcctcagagagcccctcagct


gctcccacatggccgtgggctgcccagagccactgcagccccacccgcca


ccctggctcgcgcattaagcccaccgagaacaacgaagacttcaacatcg


tggccgtggccagcaacttcgcgaccacggatctcgatgctgaccgcggg


aagttgcccggcaagaagctgccgctggaggtgctcaaagagatggaagc


caatgcccggaaagctggctgcaccaggggctgtctgatctgcctgtccc


acatcaagtgcacgcccaagatgaagaagttcatcccaggacgctgccac


acctacgaaggcgacaaagagtccgcacagggcggcataggcgaggcgat


cgtcgacattcctgagattcctgggttcaaggacttggagcccatggagc


agttcatcgcacaggtcgatctgtgtgtggactgcacaactggctgcctc


aaagggcttgccactcgtgcagtgttctgacctgctcaagaagtggctgc


cgcaacgctgtgcgacctttgccagcaagatccagggccaggtggacaag


atcaagggggccggtggtgactcgcgcattgtcctatcgctggatgtccc


catcggcctcttgcagatcttactggagcaagcccgggccagggctgcca


gggagcaggccaccaccaacgcccgcatcctggcccgtgtcggccactgc


tga


 


Urocortin-1 Mouse (nucleic acid sequence):


(SEQ ID NO: 203)


Atgatacagaggggacgcgctacgctcctggtggcgttgctgctcttggc


acagcttcgcccggagagcagccagtggagcccagcggctgcggcggcaa


ctggggtccaggatccgaatctgcgatggagccctggagtgcggaatcag


ggcggcggcgtccgcgcgctcctcttgctgttagcggagcgcttcccgcg


ccgcgcaggatctgagcctgcgggcgagcggcagcgacggaagcccaccg


agaacaacgaagacttcaacatcgtggccgtggccagcaacttcgcgacc


acggatctcgatgctgaccgcgggaagttgcccggcaagaagctgccgct


ggaggtgctcaaagagatggaagccaatgcccggaaagctggctgcacca


ggggctgtctgatctgcctgtcccacatcaagtgcacgcccaagatgaag


aagttcatcccaggacgctgccacacctacgaaggcgacaaagagtccgc


acagggcggcataggcgaggcgatcgtcgacattcctgagattcctgggt


tcaaggacttggagcccatggagcagttcatcgcacaggtcgatctgtgt


gtggactgcacaactggctgcctcaaagggcttgccaacgtgcagtgttc


tgacctgctcaagaagtggctgccgcaacgctgtgcgacctttgccagca


agatccagggccaggtggacaagatcaagggggccggtggtgaccgacgg


gacgaccctccactgtccatcgacctcaccttccacctgctgcggaccct


gctggagctagctcggacacagagccagcgcgagcgcgcagagcagaacc


gcatcatattcgattcggtgggcaagtga


 


Urocortin-1 Human (nucleic acid sequence):


(SEQ ID NO: 204)


atgaggcaggcgggacgcgcagcgctgctggccgcgctgctgctcctggt


acagctgtgccctgggagcagccagaggagccccgaggcggccggggtcc


aggacccgagtctgcgctggagccccggggcacggaaccagggtggcggg


gcccgcgcgctcctcttgctgctggcggagcgcttcccgcgccgcgcggg


gcccggccgattgggactcgggacggcaggcgagcggccgcggcggaagc


ccaccgagaacaacgaagacttcaacatcgtggccgtggccagcaacttc


gcgaccacggatctcgatgctgaccgcgggaagttgcccggcaagaagct


gccgctggaggtgctcaaagagatggaagccaatgcccggaaagctggct


gcaccaggggctgtctgatctgcctgtcccacatcaagtgcacgcccaag


atgaagaagttcatcccaggacgctgccacacctacgaaggcgacaaaga


gtccgcacagggcggcataggcgaggcgatcgtcgacattcctgagattc


ctgggttcaaggacttggagcccatggagcagttcatcgcacaggtcgat


ctgtgtgtggactgcacaactggctgcctcaaagggcttgccaacgtgca


gtgttctgacctgctcaagaagtggctgccgcaacgctgtgcgacctttg


ccagcaagatccagggccaggtggacaagatcaagggggccggtggtgac


cggcgggacaacccttctctgtccattgacctcacctttcacctgctgcg


gaccctgctggagctggcgcggacgcagagccagcgggagcgcgccgagc


agaaccgcatcatattcgactcggtgggcaagtga


 


FGF23 Mouse (nucleic acid sequence):


(SEQ ID NO: 205)


Atgctagggacctgccttagactcctggtgggcgtgctctgcactgtctg


cagcttgggcactgctagagcctatccggacacttccccattgcttggct


ccaactggggaagcctgacccacctgtacacggctacagccaggaccagc


tatcacctacagatccatagggatggtcatgtagatggcaccccccatca


gaccatctacagtgccctgatgattacatcagaggacgccggctctgtgg


tgataacaggagccatgactcgaaggttcctttgtatggatctccacggc


aacatttttggatcgcttcacttcagcccagagaattgcaagttccgcca


gtggacgctggagaatggctatgacgtctacttgtcgcagaagcatcact


acctggtgagcctgggccgcgccaagcgcatcttccagccgggcaccaac


ccgccgcccttctcccagttcctggctcgcaggaacgaggtcccgctgct


gcatttctacactgttcgcccacggcgccacacgcgcagcaagcccaccg


agaacaacgaagacttcaacatcgtggccgtggccagcaacttcgcgacc


acggatctcgatgctgaccgcgggaagttgcccggcaagaagctgccgct


ggaggtgctcaaagagatggaagccaatgcccggaaagctggctgcacca


ggggctgtctgatctgcctgtcccacatcaagtgcacgcccaagatgaag


aagttcatcccaggacgctgccacacctacgaaggcgacaaagagtccgc


acagggcggcataggcgaggcgatcgtcgacattcctgagattcctgggt


tcaaggacttggagcccatggagcagttcatcgcacaggtcgatctgtgt


gtggactgcacaactggctgcctcaaagggcttgccaacgtgcagtgttc


tgacctgctcaagaagtggctgccgcaacgctgtgcgacctttgccagca


agatccagggccaggtggacaagatcaagggggccggtggtgaccgcagc


gccgaggacccaccggagcgcgacccactgaacgtgctcaagccgcggcc


ccgcgccacgcctgtgcctgtatcctgctctcgcgagctgccgagcgcag


aggaaggtggccccgcagccagcgatcctctgggggtgctgcgcagaggc


cgtggagatgctcgcgggggcgcgggaggcgcggataggtgtcgcccctt


tcccaggttcgtctag


 


Human (nucleic acid sequence):


(SEQ ID NO: 206)


Atgttgggggcccgcctcaggctctgggtctgtgccttgtgcagcgtctg


cagcatgagcgtcctcagagcctatcccaatgcctccccactgctcggct


ccagctggggtggcctgatccacctgtacacagccacagccaggaacagc


taccacctgcagatccacaagaatggccatgtggatggcgcaccccatca


gaccatctacagtgccctgatgatcagatcagaggatgctggctttgtgg


tgattacaggtgtgatgagcagaagatacctctgcatggatttcagaggc


aacatttttggatcacactatttcgacccggagaactgcaggttccaaca


ccagacgctggaaaacgggtacgacgtctaccactctcctcagtatcact


tcctggtcagtctgggccgggcgaagagagccttcctgccaggcatgaac


ccacccccgtactcccagttcctgtcccggaggaacgagatccccctaat


tcacttcaacacccccataccacggcggcacacccggagcaagcccaccg


agaacaacgaagacttcaacatcgtggccgtggccagcaacttcgcgacc


acggatctcgatgctgaccgcgggaagttgcccggcaagaagctgccgct


ggaggtgctcaaagagatggaagccaatgcccggaaagctggctgcacca


ggggctgtctgatctgcctgtcccacatcaagtgcacgcccaagatgaag


aagttcatcccaggacgctgccacacctacgaaggcgacaaagagtccgc


acagggcggcataggcgaggcgatcgtcgacattcctgagattcctgggt


tcaaggacttggagcccatggagcagttcatcgcacaggtcgatctgtgt


gtggactgcacaactggctgcctcaaagggcttgccaacgtgcagtgttc


tgacctgctcaagaagtggctgccgcaacgctgtgcgacctttgccagca


agatccagggccaggtggacaagatcaagggggccggtggtgaccggagc


gccgaggacgactcggagcgggaccccctgaacgtgctgaagccccgggc


ccggatgaccccggccccggcctcctgttcacaggagctcccgagcgccg


aggacaacagcccgatggccagtgacccattaggggtggtcaggggcggt


cgagtgaacacgcacgctgggggaacgggcccggaaggctgccgcccctt


cgccaagttcatctag


 


IL1B Mouse (nucleic acid sequence):


(SEQ ID NO: 207)


Atggcaactgttcctgaactcaactgtgaaatgccaccttttgacagtga


tgagaatgacctgttctttgaagttgacggaccccaaaagatgaagggct


gcttccaaacctttgacctgggctgtcctgatgagagcatccagcttcaa


atctcgcagcagcacatcaacaagagcttcaggcaggcagtatcactcat


tgtggctgtggagaagctgtggcagctacctgtgtctttcccgtggacct


tccaggatgaggacatgagcaccttcttttccttcatctttgaagaagag


cccatcctctgtgactcatgggatgatgatgataacctgctggtgtgtga


cgttcccaagcccaccgagaacaacgaagacttcaacatcgtggccgtgg


ccagcaacttcgcgaccacggatctcgatgctgaccgcgggaagttgccc


ggcaagaagctgccgctggaggtgctcaaagagattggaagccaatgccc


ggaaagctggctgcaccaggggctgtctgatctgcctgtcccacatcaag


tgcacgcccaagatgaagaagttcatcccaggacgctgccacacctacga


aggcgacaaagagtccgcacagggcggcataggcgaggcgatcgtcgaca


ttcctgagattcctgggttcaaggacttggagcccatggagcagttcatc


gcacaggtcgatctgtgtgtggactgcacaactggctgcctcaaagggct


tgccaacgtgcagtgttctgacctgctcaagaagtggctgccgcaacgct


gtgcgacctttgccagcaagatccagggccaggtggacaagatcaagggg


gccggtggtgacctggtgtgtgacgttcccgttcccattagacaactgca


ctacaggctccgagatgaacaacaaaaaagcctcgtgctgtcggacccat


atgagctgaaagctctccacctcaatggacagaatatcaaccaacaagtg


atattctccatgagctttgtacaaggagaaccaagcaacgacaaaatacc


tgtggccttgggcctcaaaggaaagaatctatacctgtcctgtgtaatga


aagacggcacacccaccctgcagctggagagtgtggatcccaagcaatac


ccaaagaagaagatggaaaaacggtttgtcttcaacaagatagaagtcaa


gagcaaagtggagtttgagtctgcagagttccccaactggtacatcagca


cctcacaagcagagcacaagcctgtcttcctgggaaacaacagtggtcag


gacataattgacttcaccatggaatccgtgtcttcctaa


 


IL1B Human (nucleic acid sequence):


(SEQ ID NO: 208)


Atggcagaagtacctgagctcgccagtgaaatgatggcttattacagtgg


caatgaggatgacttgttctttgaagctgatggccctaaacagatgaagt


gctccttccaggacctggacctctgccctctggatggcggcatccagcta


cgaatctccgaccaccactacagcaagggcttcaggcaggccgcgtcagt


tgttgtggccatggacaagctgaggaagatgctggttccctgcccacaga


ccttccaggagaatgacctgagcaccttctttcccttcatctttgaagaa


gaacctatcttcttcgacacatgggataacgaggcttatgtgcacgatgc


acctaagcccaccgagaacaacgaagacttcaacatcgtggccgtggcca


gcaacttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggc


aagaagctgccgctggaggtgctcaaagagatggaagccaatgcccggaa


agctggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgca


cgcccaagatgaagaagttcatcccaggacgctgccacacctacgaaggc


gacaaagagtccgcacagggcggcataggcgaggcgatcgtcgacattcc


tgagattcctgggttcaaggacttggagcccatggagcagttcatcgcac


aggtcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgcc


aacgtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgc


gacctttgccagcaagatccagggccaggtggacaagatcaagggggccg


gtggtgactatgtgcacgatgcacctgtacgatcactgaactgcacgctc


cgggactcacagcaaaaaagcttggtgatgtctggtccatatgaactgaa


agctctccacctccagggacaggatatggagcaacaagtggtgttctcca


tgtcctttgtacaaggagaagaaagtaatgacaaaatacctgtggccttg


ggcctcaaggaaaagaatctgtacctgtcctgcgtgttgaaagatgataa


gcccactctacagctggagagtgtagatcccaaaaattacccaaagaaga


agatggaaaagcgatttgtcttcaacaagatagaaatcaataacaagctg


gaatttgagtctgcccagttccccaactggtacatcagcacctctcaagc


agaaaacatgcccgtcttcctgggagggaccaaaggcggccaggatataa


ctgacttcaccatgcaatttgtgtcttcctaa


 


TNFA Mouse (nucleic acid sequence):


(SEQ ID NO: 209)


Atgagcacagaaagcatgatccgcgacgtggaactggcagaagaggcact


cccccaaaagatggggggcttccagaactccaggcggtgcctatgtctca


gcctcttctcattcctgcttgtggcaggggccaccacgctcttctgtcta


ctgaacttcggggtgatcggtccccaaagggatgagaagttcccaaatgg


cctccctctcatcagttctatggcccagaccctcacactcagaaagccca


ccgagaacaacgaagacttcaacatcgtggccgtggccagcaacttcgcg


accacggatctcgatgctgaccgcgggaagttgcccggcaagaagctgcc


gctggaggtgctcaaagagatggaagccaatgcccggaaagctggctgca


ccaggggctgtctgatctgcctgtcccacatcaagtgcacgcccaagatg


aagaagttcatcccaggacgctgccacacctacgaaggcgacaaagagtc


cgcacagggcggcataggcgaggcgatcgtcgacattcctgagattcctg


ggttcaaggacttggagcccatggagcagttcatcgcacaggtcgatctg


tgtgtggactgcacaactggctgcctcaaagggcttgccaacgtgcagtg


ttctgacctgctcaagaagtggctgccgcaacgctgtgcgacctttgcca


gcaagatccagggccaggtggacaagatcaagggggccggtggtgacctc


agatcatcttctcaaaattcgagtgacaagcctgtagcccacgtcgtagc


aaaccaccaagtggaggagcagctggagtggctgagccagcgcgccaacg


ccctcctggccaacggcatggatctcaaagacaaccaactagtggtgcca


gccgatgggttgtaccttgtctactcccaggttctcttcaagggacaagg


ctgccccgactacgtgctcctcacccacaccgtcagccgatttgctatct


cataccaggagaaagtcaacctcctctctgccgtcaagagcccctgcccc


aaggacacccctgagggggctgagctcaaaccctggtatgagcccatata


cctgggaggagtcttccagctggagaagggggaccaactcagcgctgagg


tcaatctgcccaagtacttagactttgcggagtccgggcaggtctacttt


ggagtcattgctctgtga


 


TNFA Human (nucleic acid sequence):


(SEQ ID NO: 210)


Atgagcactgaaagcatgatccgggacgtggagctggccgaggaggcgct


ccccaagaagacaggggggccccagggctccaggcggtgcttgttcctca


gcctcttctccttcctgatcgtggcaggcgccaccacgctcttctgcctg


ctgcactttggagtgatcggcccccagagggaagagttccccagggacct


ctctctaatcagccctctggcccaggcagtcagaaagcccaccgagaaca


acgaagacttcaacatcgtggccgtggccagcaacttcgcgaccacggat


ctcgatgctgaccgcgggaagttgcccggcaagaagctgccgctggaggt


gctcaaagagatggaagccaatgcccggaaagctggctgcaccaggggct


gtctgatctgcctgtcccacatcaagtgcacgcccaagatgaagaagttc


atcccaggacgctgccacacctacgaaggcgacaaagagtccgcacaggg


cggcataggcgaggcgatcgtcgacattcctgagattcctgggttcaagg


acttggagcccatggagcagttcatcgcacaggtcgatctgtgtgtggac


tgcacaactggctgcctcaaagggcttgccaacgtgcagtgttctgacct


gctcaagaagtggctgccgcaacgctgtgcgacctttgccagcaagatcc


agggccaggtggacaagatcaagggggccggtggtgacgtcagatcatct


tctcgaaccccgagtgacaagcctgtagcccatgttgtagcaaaccctca


agctgaggggcagctccagtggctgaaccgccgggccaatgccctcctgg


ccaatggcgtggagctgagagataaccagctggtggtgccatcagagggc


ctgtacctcatctactcccaggtcctcttcaagggccaaggctgcccctc


cacccatgtgctcctcacccacaccatcagccgcatcgccgtctcctacc


agaccaaggtcaacctcctctctgccatcaagagcccctgccagagggag


accccagagggggctgaggccaagccctggtatgagcccatctatctggg


aggggtcttccagctggagaagggtgaccgactcagcgctgagatcaatc


ggcccgactatctcgactttgccgagtctgggcaggtctactttgggatc


attgccctgtga


 


IFNG Mouse (nucleic acid sequence):


(SEQ ID NO: 211)


Atgaacgctacacactgcatcttggctttgcagctcttcctcatggctgt


ttctggctgttactgccacggcacagtcattgaaagcctagaaagtctga


ataactattttaactcaagtggcatagatgtggaagaaaagagtctcttc


ttggatatctggaggaactggcaaaaggatggtgacatgaaaatcctgca


gagccagattatctctttctacctcagactctttgaagtcttgaaagaca


atcaggccatcagcaacaacataagcgtcattgaatcacacctgattact


accttcttcagcaacagcaaggcgaaaaaggatgcattcatgagtattgc


caagtttgaggtcaacaacccacaggtccagcgccaagcattcaatgagc


tcatccgagtggtccaccagctgttgccggaatccagcctcaggaagaag


cccaccgagaacaacgaagacttcaacatcgtggccgtggccagcaactt


cgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaagaagc


tgccgctggaggtgctcaaagagatggaagccaatgcccggaaagctggc


tgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacgcccaa


gatgaagaagttcatcccaggacgctgccacacctacgaaggcgacaaag


agtccgcacagggcggcataggcgaggcgatcgtcgacattcctgagatt


cctgggttcaaggacttggagcccatggagcagttcatcgcacaggtcga


tctgtgtgtggactgcacaactggctgcctcaaagggcttgccaacgtgc


agtgttctgacctgctcaagaagtggctgccgcaacgctgtgcgaccttt


gccagcaagatccagggccaggtggacaagatcaagggggccggtggtga


ccggaaaaggagtcgctgctga


 


IFNG Human (nucleic acid sequence):


(SEQ ID NO: 212)


Atgaaatatacaagttatatcttggcttttcagctctgcatcgttttggg


ttctcttggctgttactgccaggacccatatgtaaaagaagcagaaaacc


ttaagaaatattttaatgcaggtcattcagatgtagcggataatggaact


cttttcttaggcattttgaagaattggaaagaggagagtgacagaaaaat


aatgcagagccaaattgtctccttttacttcaaactttttaaaaacttta


aagatgaccagagcatccaaaagagtgtggagaccatcaaggaagacatg


aatgtcaagtttttcaatagcaacaaaaagaaacgagatgacttcgaaaa


gctgactaattattcggtaactgacttgaatgtccaacgcaaagcaatac


atgaactcatccaagtgatggctgaactgtcgccagcagctaaaacaggg


aagcgaaaaaggagtcagatgctgtttcgaggtcgaagaaagcccaccga


gaacaacgaagacttcaacatcgtggccgtggccagcaacttcgcgacca


cggatctcgatgctgaccgcgggaagttgcccggcaagaagctgccgctg


gaggtgctcaaagagatggaagccaatgcccggaaagctggctgcaccag


gggctgtctgatctgcctgtcccacatcaagtgcacgcccaagatgaaga


agttcatcccaggacgctgccacacctacgaaggcgacaaagagtccgca


cagggcggcataggcgaggcgatcgtcgacattcctgagattcctgggtt


caaggacttggagcccatggagcagttcatcgcacaggtcgatctgtgtg


tggactgcacaactggctgcctcaaagggcttgccaacgtgcagtgttct


gacctgctcaagaagtggctgccgcaacgctgtgcgacctttgccagcaa


gatccagggccaggtggacaagatcaagggggccggtggtgacggtcgaa


gagcatcccagtaa


 


Sortilin Mouse (nucleic acid sequence):


(SEQ ID NO: 213)


Atggagcggccccggggagctgcggacggccttttgcgctggcccctcgg


cctcctcctgctccttcaactgctgcctcctgccgccgtcggccaggacc


ggctggacgcgccgccgccgcccgcgcctcctctgctgcgctgggccggt


ccggtcggggtgagctgggggctgcgcgccgccgcgcccgggggccccgt


cccccgcgctggccgttggcgccgcaagcccaccgagaacaacgaagact


tcaacatcgtggccgtggccagcaacttcgcgaccacggatctcgatgct


gaccgcgggaagttgcccggcaagaagctgccgctggaggtgctcaaaga


gatggaagccaatgcccggaaagctggctgcaccaggggctgtctgatct


gcctgtcccacatcaagtgcacgcccaagatgaagaagttcatcccagga


cgctgccacacctacgaaggcgacaaagagtccgcacagggcggcatagg


cgaggcgatcgtcgacattcctgagattcctgggttcaaggacttggagc


ccatggagcagttcatcgcacaggtcgatctgtgtgtggactgcacaact


ggctgcctcaaagggcttgccaacgtgcagtgttctgacctgctcaagaa


gtggctgccgcaacgctgtgcgacctttgccagcaagatccagggccagg


tggacaagatcaagggggccggtggtgaccgccgcggcgcgcccgccgag


gaccaagactgcggccgcctcccggacttcatcgccaagctgaccaacaa


tacgcaccagcatgtctttgatgacctcagtggctcagtgtccttgtcct


gggttggagacagcactggggttattctcgtcctgaccactttccaagtg


cctctggtaattgtgagctttggacagtccaagttgtatcgaagtgagga


ttatggaaagaactttaaggatattacaaatctcatcaataacaccttca


ttcggacggaatttggcatggctattggtcctgagaactctggaaaggtg


atactaacagcggaggtgtccgggggaagccgaggcggaagagtgttcag


gtcatcagactttgccaagaactttgtgcaaacagatctcccctttcatc


ctctgacgcagatgatgtacagccctcagaattctgattacctgttagct


ctcagcaccgaaaatggcctgtgggtgtccaagaattttggggaaaaatg


ggaagaaatccacaaagcagtatgtttggccaaatggggaccaaacaaca


tcatcttctttaccacccatgtgaatggctcctgcaaagctgatcttggt


gccctggaattatggagaacatccgacttgggaaaaaccttcaaaaccat


tggtgtgaaaatctactcctttggtcttgggggccgtttcctttttgcct


ctgtgatggctgataaggacacaacaagaaggatccatgtgtcaacagac


cagggggacacatggagcatggcacaacttccttctgtgggacaggaaca


gttctactccatcctggcagccaatgaggacatggtcttcatgcatgtag


atgaacctggagataccgggtttggcaccatctttacctctgatgatcga


ggcattgtctactccaagtctctggacagacatctctataccaccacagg


cggggagacggactttaccaacgtgacttccctccgtggggtctatataa


caagcacgctctcagaagataactctattcagagcatgatcacttttgac


cagggaggacggtgggagcacctgcggaagccggagaacagcaagtgcga


cgctaccgcaaagaacaagaacgagtgcagccttcatatccatgcttctt


atagcatctcccagaagctaaacgttccaatggccccactttccgagccc


aatgctgtgggcatagtcatcgctcacggtagtgtgggagatgccatctc


ggtgatggtcccagatgtgtacatctcagatgatgggggttactcctggg


cgaagatgctagaaggaccacattactataccatcctggactctggaggc


atcattgtggccattgagcacagcaaccgtcctatcaatgtgattaagtt


ctccacagatgaaggccagtgctggcagagctatgtgttcacacaggagc


ccatctacttcactgggcttgcttccgagcctggagccaggtccatgaac


atcagcatctggggattcacagagtctttcattacccgccagtgggtctc


ctacacagtcgatttcaaagacatccttgagcggaattgtgaagaggatg


actataccacgtggctggcacactccacagaccctggagattacaaagac


ggctgcattttgggctataaagaacagttcctacggctacggaagtcatc


cgtctgtcagaatggtcgagactatgttgtggccaagcagccatccgtct


gtccgtgttccctggaggacttcctctgtgactttggctacttccgtccg


gagaacgcctcagagtgcgtggagcagcctgaactgaaggggcatgagtt


agagttctgtctgtacggcaaggaggagcacctgacaacaaatgggtacc


ggaaaatcccaggagacaaatgccaaggtgggatgaatcccgccagagaa


gtaaaagacttgaaaaagaaatgcacaagcaacttcttgaaccccacaaa


gcaggactcccgcccacagggacacagcttgtcccagaatccagctccgc


ctcctcttggatacactgaaaacacacacttcctatctcctacccagaag


cagaattccaagtcaaattctgtccctattatcctggccatcgtgggact


gatgcttgtcacagtcgtagcaggagtcctcattgtgaagaaatatgtct


gtggcggaaggttcctggtgcaccggtactcggtgctacagcagcacgca


gaggctgacggcgtagaggctttggattcaacctcccacgctaaaagcgg


atatcacgacgactcagatgaggacctcctggaatag


 


Sortilin Human (nucleic acid sequence):


(SEQ ID NO: 214)


Atggagcggccctggggagctgcggacggcctctcgcgctggccccatgg


cctcggcctcctcctcctcctgcagctgctgccgccgtcgaccctcagcc


aggaccggctggacgcgccgccgccgcccgctgcgccgctgccgcgctgg


tctggccccatcggggtgagctgggggctgcgggcggccgcagccggggg


cgcgtttccccgcggcggccgttggcgtcgcaagcccaccgagaacaacg


aagacttcaacatcgtggccgtggccagcaacttcgcgaccacggatctc


gatgctgaccgcgggaagttgcccggcaagaagctgccgctggaggtgct


caaagagatggaagccaatgcccggaaagctggctgcaccaggggctgtc


tgatctgcctgtcccacatcaagtgcacgcccaagatgaagaagttcatc


ccaggacgctgccacacctacgaaggcgacaaagagtccgcacagggcgg


cataggcgaggcgatcgtcgacattcctgagattcctgggttcaaggact


tggagcccatggagcagttcatcgcacaggtcgatctgtgtgtggactgc


acaactggctgcctcaaagggcttgccaacgtgcagtgttctgacctgct


caagaagtggctgccgcaacgctgtgcgacctttgccagcaagatccagg


gccaggtggacaagatcaagggggccggtggtgaccgtcgcagcgcgccg


ggcgaggacgaggagtgcggccgggtccgggacttcgtcgccaagctggc


caacaacacgcaccagcatgtgtttgatgatctcagaggctcagtatcct


tgtcctgggttggagatagcactggggtcattctagtcttgactaccttc


catgtaccactggtaattatgacttttggacagtccaagctatatcgaag


tgaggattatgggaagaactttaaggatattacagatctcatcaataaca


cctttattcggactgaatttggcatggctattggtcctgagaactctgga


aaggtggtgttaacagcagaggtgtctggaggaagtcgtggaggaagaat


ctttagatcatcagattttgcgaagaattttgtgcaaacagatctccctt


ttcatcctctcactcagatgatgtatagccctcagaattctgattatctt


ttagctctcagcactgaaaatggcctgtgggtgtccaagaattttggggg


aaaatgggaagaaatccacaaagcagtatgtttggccaaatggggatcag


acaacaccatcttctttacaacctatgcaaatggctcctgcaaagctgac


cttggggctctggaattatggagaacttcagacttgggaaaaagcttcaa


aactattggtgtgaaaatctactcatttggtcttgggggacgtttccttt


ttgcctctgtgatggctgataaggatacaacaagaaggatccacgtttca


acagatcaaggggacacatggagcatggcccagctcccctccgtgggaca


ggaacagttctattctattctggcagcaaatgatgacatggtattcatgc


atgtagatgaacctggagacactgggtttggcacaatctttacctcagat


gatcgaggcattgtctattccaagtctttggaccgacatctctacactac


cacaggcggagagacggactttaccaacgtgacctccctccgcggcgtct


acataacaagcgtgctctccgaagataattctatccagaccatgatcact


tttgaccaaggaggaaggtggacgcacctgaggaagcctgaaaacagtga


atgtgatgctacagcaaaaaacaagaatgagtgcagccttcatattcatg


cttcctacagcatctcccagaaactgaatgttccaatggccccactctca


gagccgaatgccgtaggcattgtcattgctcatggtagcgtgggggatgc


catctcagtgatggttccagatgtgtacatctcagatgatgggggttact


cctggacaaagatgctggaaggaccccactattacaccatcctggattct


ggaggcatcattgtggccattgagcacagcagccgtcctatcaatgtgat


taagttctccacagacgaaggtcaatgctggcaaacctacacgttcacca


gggaccccatctatttcactggcctagcttcagaacctggagctaggtcc


atgaatatcagcatttggggcttcacagaatctttcctgaccagccagtg


ggtctcctacaccattgattttaaagatatccttgaaaggaactgtgaag


agaaggactataccatatggctggcacactccacagaccctgaagattat


gaagatggctgcattttgggctacaaagaacagtttctgcggctacgcaa


gtcatccgtgtgtcagaatggtcgagactatgttgtgaccaagcagccct


ccatctgcctctgttccctggaggactttctctgtgattttggctactac


cgtccagaaaatgactccaagtgtgtggaacagccagaactgaagggcca


cgacctggagttttgtctgtacggaagagaagaacacctaacaacaaatg


ggtaccggaaaattccaggggacaaatgccagggtggggtaaatccagtt


cgagaagtaaaagacttgaaaaagaaatgcacaagcaactttttgagtcc


ggaaaaacagaattccaagtcaaattctgttccaattatcctggccatcg


tgggattgatgctggtcacagtcgtagcaggagtgctcattgtgaagaaa


tatgtctgtgggggaaggttcctggtgcatcgatactctgtgctgcagca


gcatgcagaggccaatggtgtggatggtgtggatgctttggacacagcct


cccacactaataaaagtggttatcatgatgactcagatgaggacctcttg


gaatag


 


Neuropeptide W Mouse (nucleic acid sequence):


(SEQ ID NO: 215)


Ctggcgtctaacagagaagtgcggggccctgggcccgggactcccaggaa


ccggcccctgctgcccctgctgctgcttctgctcttgctaccgctgcccg


ccagcgcctggtataagcacgtggcgagtccccgctatcacacagtgggt


cgtgcctccgggctgctcatggggctgcgccgctcgccctaccagtggcg


ccgtaagcccaccgagaacaacgaagacttcaacatcgtggccgtggcca


gcaacttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggc


aagaagctgccgctggaggtgctcaaagagatggaagccaatgcccggaa


agctggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgca


cgcccaagatgaagaagttcatcccaggacgctgccacacctacgaaggc


gacaaagagtccgcacagggcggcataggcgaggcgatcgtcgacattcc


tgagattcctgggttcaaggacttggagcccatggagcagttcatcgcac


aggtcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgcc


aacgtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgc


gacctttgccagcaagatccagggccaggtggacaagatcaagggggccg


gtggtgaccgccgtgccctgggcggggctgctggacccctctcccggctc


ccaggaccggtcgcccgcggcgctctcctgcttccttcctcagggcagga


gctgtgggaggtacgaagcaggagctcacctgcagggcttcccgtccatg


caccctggagtccgcgggacctggagggagtccgccaaccggagcagtcg


ctaagccttcactcctggatctcagaggagcccgctgctagagccttcgg


agagacgcttcgtgcccagccatggttcctgcagcaagtcatctttgccg


atcctgtcaggcccaagaaccgatggcgcccccatgcttga


 


Neuropeptide W Human (nucleic acid sequence):


(SEQ ID NO: 216)


Ctggcgtggcgcccaggggagcggggggctcccgcgagccggccgcggct


ggcactgctgctgcttctgctcctgctgccgctgccctccggcgcgtggt


acaagcacgtggcgagtccccgctaccacacggtgggccgcgccgctggc


ctgctcatggggctgcgtcgctcaccctatctgtggcgccgcaagcccac


cgagaacaacgaagacttcaacatcgtggccgtggccagcaacttcgcga


ccacggatctcgatgctgaccgcgggaagttgcccggcaagaagctgccg


ctggaggtgctcaaagagatggaagccaatgcccggaaagctggctgcac


caggggctgtctgatctgcctgtcccacatcaagtgcacgcccaagatga


agaagttcatcccaggacgctgccacacctacgaaggcgacaaagagtcc


gcacagggcggcataggcgaggcgatcgtcgacattcctgagattcctgg


gttcaaggacttggagcccatggagcagttcatcgcacaggtcgatctgt


gtgtggactgcacaactggctgcctcaaagggcttgccaacgtgcagtgt


tctgacctgctcaagaagtggctgccgcaacgctgtgcgacctttgccag


caagatccagggccaggtggacaagatcaagggggccggtggtgaccgcc


gcgcgctgcgcgcggccgccgggcccctggccagggacaccctctccccc


gaacccgcagcccgcgaggctcctctcctgctgccctcgtgggttcagga


gctgtgggagacgcgacgcaggagctcccaggcagggatccccgtccgtg


cgccccggagcccgcgcgccccagagcctgcgctggaaccggagtccctg


gacttcagcggagctggccagagacttcggagagacgtctcccgcccagc


ggtggaccccgcagcaaaccgccttggcctgccctgcctggcccccggac


cgttctga


 


CART Mouse (nucleic acid sequence):


(SEQ ID NO: 217)


Atggagagctcccgcctgcggctgctacccctcctgggcgccgccctgct


gctactgctacctttgctgggtgcccgtgcccaggaggacgccgagctgc


agccccgagccctggacatctactctgccgtggatgatgcgtcccacgag


aaggagctgatcgaagcgttgcaagaagtcctgaagaagctcaagagtaa


acgcaagcccaccgagaacaacgaagacttcaacatcgtggccgtggcca


gcaacttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggc


aagaagctgccgctggaggtgctcaaagagatggaagccaatgcccggaa


agctggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgca


cgcccaagatgaagaagttcatcccaggacgctgccacacctacgaaggc


gacaaagagtccgcacagggcggcataggcgaggcgatcgtcgacattcc


tgagattcctgggttcaaggacttggagcccatggagcagttcatcgcac


aggtcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgcc


aacgtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgc


gacctttgccagcaagatccagggccaggtggacaagatcaagggggccg


gtggtgacaaacgcattccgatctacgagaagaagtacggccaagtcccc


atgtgtgacgctggagagcagtgcgcagtgaggaaaggggccaggatcgg


gaagctgtgtgactgtccccgaggaacttcctgcaattctttcctcttga


agtgcttgtga


 


CART Human (nucleic acid sequence):


(SEQ ID NO: 218)


Atggagagctcccgcgtgaggctgctgcccctcctgggcgccgccctgct


gctgatgctacctctgttgggtacccgtgcccaggaggacgccgagctcc


agccccgagccctggacatctactctgccgtggatgatgcctcccacgag


aaggagctgatcgaagcgctgcaagaagtcttgaagaagctcaagagtaa


acgtaagcccaccgagaacaacgaagacttcaacatcgtggccgtggcca


gcaacttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggc


aagaagctgccgctggaggtgctcaaagagatggaagccaatgcccggaa


agctggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgca


cgcccaagatgaagaagttcatcccaggacgctgccacacctacgaaggc


gacaaagagtccgcacagggcggcataggcgaggcgatcgtcgacattcc


tgagattcctgggttcaaggacttggagcccatggagcagttcatcgcac


aggtcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgcc


aacgtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgc


gacctttgccagcaagatccagggccaggtggacaagatcaagggggccg


gtggtgacaaacgtgttcccatctatgagaagaagtatggccaagtcccc


atgtgtgacgccggtgagcagtgtgcagtgaggaaaggggcaaggatcgg


gaagctgtgtgactgtccccgaggaacctcctgcaattccttcctcctga


agtgcttatga


 


TGFB1 Mouse (nucleic acid sequence):


(SEQ ID NO: 219)


Atgccgccctcggggctgcggctactgccgcttctgctcccactcccgtg


gcttctagtgctgacgcccgggaggccagccgcgggactctccacctgca


agaccatcgacatggagctggtgaaacggaagcgcatcgaagccatccgt


ggccagatcctgtccaaactaaggctcgccagtcccccaagccaggggga


ggtaccgcccggcccgctgcccgaggcggtgctcgctttgtacaacagca


cccgcgaccgggtggcaggcgagagcgccgacccagagccggagcccgaa


gcggactactatgctaaagaggtcacccgcgtgctaatggtggaccgcaa


caacgccatctatgagaaaaccaaagacatctcacacagtatatatatgt


tcttcaatacgtcagacattcgggaagcagtgcccgaacccccattgctg


tcccgtgcagagctgcgcttgcagagattaaaatcaagtgtggagcaaca


tgtggaactctaccagaaatatagcaacaattcctggcgttaccttggta


accggctgctgacccccactgatacgcctgagtggctgtcttttgacgtc


actggagttgtacggcagtggctgaaccaaggagacggaatacagggctt


tcgattcagcgctcactgctcttgtgacagcaaagataacaaactccacg


tggaaatcaacgggatcagccccaaacgtcggggcgacctgggcaccatc


catgacatgaaccggcccttcctgctcctcatggccacccccctggaaag


ggcccagcacctgcacagctcacggcaccggagaaagcccaccgagaaca


acgaagacttcaacatcgtggccgtggccagcaacttcgcgaccacggat


ctcgatgctgaccgcgggaagttgcccggcaagaagctgccgctggaggt


gctcaaagagatggaagccaatgcccggaaagctggctgcaccaggggct


gtctgatctgcctgtcccacatcaagtgcacgcccaagatgaagaagttc


atcccaggacgctgccacacctacgaaggcgacaaagagtccgcacaggg


cggcataggcgaggcgatcgtcgacattcctgagattcctgggttcaagg


acttggagcccatggagcagttcatcgcacaggtcgatctgtgtgtggac


tgcacaactggctgcctcaaagggcttgccaacgtgcagtgttctgacct


gctcaagaagtggctgccgcaacgctgtgcgacctttgccagcaagatcc


agggccaggtggacaagatcaagggggccggtggtgaccggagagccctg


gataccaactattgcttcagctccacagagaagaactgctgtgtgcggca


gctgtacattgactttaggaaggacctgggttggaagtggatccacgagc


ccaagggctaccatgccaacttctgtctgggaccctgcccctatatttgg


agcctggacacacagtacagcaaggtccttgccctctacaaccaacacaa


cccgggcgcttcggcgtcaccgtgctgcgtgccgcaggctttggagccac


tgcccatcgtctactacgtgggtcgcaagcccaaggtggagcagttgtcc


aacatgattgtgcgctcctgcaagtgcagctga


 


TGFBl Human (nucleic acid sequence):


(SEQ ID NO: 220)


Atgccgccctccgggctgcggctgctgccgctgctgctaccgctgctgtg


gctactggtgctgacgcctggccggccggccgcgggactatccacctgca


agactatcgacatggagctggtgaagcggaagcgcatcgaggccatccgc


ggccagatcctgtccaagctgcggctcgccagccccccgagccaggggga


ggtgccgcccggcccgctgcccgaggccgtgctcgccctgtacaacagca


cccgcgaccgggtggccggggagagtgcagaaccggagcccgagcctgag


gccgactactacgccaaggaggtcacccgcgtgctaatggtggaaaccca


caacgaaatctatgacaagttcaagcagagtacacacagcatatatatgt


tcttcaacacatcagagctccgagaagcggtacctgaacccgtgttgctc


tcccgggcagagctgcgtctgctgaggctcaagttaaaagtggagcagca


cgtggagctgtaccagaaatacagcaacaattcctggcgatacctcagca


accggctgctggcacccagcgactcgccagagtggttatcttttgatgtc


accggagttgtgcggcagtggttgagccgtggaggggaaattgagggctt


tcgccttagcgcccactgctcctgtgacagcagggataacacactgcaag


tggacatcaacgggttcactaccggccgccgaggtgacctggccaccatt


catggcatgaaccggcctttcctgcttctcatggccaccccgctggagag


ggcccagcatctgcaaagctcccggcaccgccgaaagcccaccgagaaca


acgaagacttcaacatcgtggccgtggccagcaacttcgcgaccacggat


ctcgatgctgaccgcgggaagttgcccggcaagaagctgccgctggaggt


gctcaaagagatggaagccaatgcccggaaagctggctgcaccaggggct


gtctgatctgcctgtcccacatcaagtgcacgcccaagatgaagaagttc


atcccaggacgctgccacacctacgaaggcgacaaagagtccgcacaggg


cggcataggcgaggcgatcgtcgacattcctgagattcctgggttcaagg


acttggagcccatggagcagttcatcgcacaggtcgatctgtgtgtggac


tgcacaactggctgcctcaaagggcttgccaacgtgcagtgttctgacct


gctcaagaagtggctgccgcaacgctgtgcgacctttgccagcaagatcc


agggccaggtggacaagatcaagggggccggtggtgaccgccgagccctg


gacaccaactattgcttcagctccacggagaagaactgctgcgtgcggca


gctgtacattgacttccgcaaggacctcggctggaagtggatccacgagc


ccaagggctaccatgccaacttctgcctcgggccctgcccctacatttgg


agcctggacacgcagtacagcaaggtcctggccctgtacaaccagcataa


cccgggcgcctcggcggcgccgtgctgcgtgccgcaggcgctggagccgc


tgcccatcgtgtactacgtgggccgcaagcccaaggtggagcagctgtcc


aacatgatcgtgcgctcctgcaagtgcagctga


 


TGFB2 Mouse (nucleic acid sequence):


(SEQ ID NO: 221)


Atgcactactgtgtgctgagcacctttttgctcctgcatctggtcccggt


ggcgctcagtctgtctacctgcagcaccctcgacatggatcagtttatgc


gcaagaggatcgaggccatccgcgggcagatcctgagcaagctgaagctc


accagccccccggaagactatccggagccggatgaggtccccccggaggt


gatttccatctacaacagtaccagggacttactgcaggagaaggcaagcc


ggagggcagccgcctgcgagcgcgagcggagcgacgaggagtactacgcc


aaggaggtttataaaatcgacatgccgtcccacctcccctccgaaaatgc


catcccgcccactttctacagaccctacttcagaatcgtccgctttgatg


tctcaacaatggagaaaaatgcttcgaatctggtgaaggcagagttcagg


gtcttccgcttgcaaaaccccaaagccagagtggccgagcagcggattga


actgtatcagatccttaaatccaaagacttaacatctcccacccagcgct


acatcgatagcaaggttgtgaaaaccagagcggagggtgaatggctctcc


ttcgacgtgacagacgctgtgcaggagtggcttcaccacaaagacaggaa


cctggggtttaaaataagtttacactgcccctgctgtaccttcgtgccgt


ctaataattacatcatcccgaataaaagcgaagagctcgaggcgagattt


gcaggtattgatggcacctctacatatgccagtggtgatcagaaaactat


aaagtccactaggaaaaaaaccagtgggaagaccccacatctcctgctaa


tgttgttgccctcctacagactggagtcacaacagtccagccggcggaag


cccaccgagaacaacgaagacttcaacatcgtggccgtggccagcaactt


cgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaagaagc


tgccgctggaggtgctcaaagagatggaagccaatgcccggaaagctggc


tgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacgcccaa


gatgaagaagttcatcccaggacgctgccacacctacgaaggcgacaaag


agtccgcacagggcggcataggcgaggcgatcgtcgacattcctgagatt


cctgggttcaaggacttggagcccatggagcagttcatcgcacaggtcga


tctgtgtgtggactgcacaactggctgcctcaaagggcttgccaacgtgc


agtgttctgacctgctcaagaagtggctgccgcaacgctgtgcgaccttt


gccagcaagatccagggccaggtggacaagatcaagggggccggtggtga


ccggcggaagaagcgcgctttggatgctgcctactgctttagaaatgtgc


aggataattgctgccttcgccctctttacattgattttaagagggatctt


ggatggaaatggatccatgaacccaaagggtacaatgctaacttctgtgc


tggggcatgcccatatctatggagttcagacactcaacacaccaaagtcc


tcagcctgtacaacaccataaatcccgaagcttccgcttccccttgctgt


gtgtcccaggatctggaaccactgaccattctctattacattggaaatac


gcccaagatcgaacagctttccaatatgattgtcaagtcttgtaaatgca


gctaa


 


TGFB2 Human (nucleic acid sequence):


(SEQ ID NO: 222)


Atgcactactgtgtgctgagcgcttttctgatcctgcatctggtcacggt


cgcgctcagcctgtctacctgcagcacactcgatatggaccagttcatgc


gcaagaggatcgaggcgatccgcgggcagatcctgagcaagctgaagctc


accagtcccccagaagactatcctgagcccgaggaagtccccccggaggt


gatttccatctacaacagcaccagggacttgctccaggagaaggcgagcc


ggagggcggccgcctgcgagcgcgagaggagcgacgaagagtactacgcc


aaggaggtttacaaaatagacatgccgcccttcttcccctccgaaactgt


ctgcccagttgttacaacaccctctggctcagtgggcagcttgtgctcca


gacagtcccaggtgctctgtgggtaccttgatgccatcccgcccactttc


tacagaccctacttcagaattgttcgatttgacgtctcagcaatggagaa


gaatgcttccaatttggtgaaagcagagttcagagtctttcgtttgcaga


acccaaaagccagagtgcctgaacaacggattgagctatatcagattctc


aagtccaaagatttaacatctccaacccagcgctacatcgacagcaaagt


tgtgaaaacaagagcagaaggcgaatggctctccttcgatgtaactgatg


ctgttcatgaatggcttcaccataaagacaggaacctgggatttaaaata


agcttacactgtccctgctgcacttttgtaccatctaataattacatcat


cccaaataaaagtgaagaactagaagcaagatttgcaggtattgatggca


cctccacatataccagtggtgatcagaaaactataaagtccactaggaaa


aaaaacagtgggaagaccccacatctcctgctaatgttattgccctccta


cagacttgagtcacaacagaccaaccggcggaagcccaccgagaacaacg


aagacttcaacatcgtggccgtggccagcaacttcgcgaccacggatctc


gatgctgaccgcgggaagttgcccggcaagaagctgccgctggaggtgct


caaagagatggaagccaatgcccggaaagctggctgcaccaggggctgtc


tgatctgcctgtcccacatcaagtgcacgcccaagatgaagaagttcatc


ccaggacgctgccacacctacgaaggcgacaaagagtccgcacagggcgg


cataggcgaggcgatcgtcgacattcctgagattcctgggttcaaggact


tggagcccatggagcagttcatcgcacaggtcgatctgtgtgtggactgc


acaactggctgcctcaaagggcttgccaacgtgcagtgttctgacctgct


caagaagtggctgccgcaacgctgtgcgacctttgccagcaagatccagg


gccaggtggacaagatcaagggggccggtggtgaccggcggaagaagcgt


gctttggatgcggcctattgctttagaaatgtgcaggataattgctgcct


acgtccactttacattgatttcaagagggatctagggtggaaatggatac


acgaacccaaagggtacaatgccaacttctgtgctggagcatgcccgtat


ttatggagttcagacactcagcacagcagggtcctgagcttatataatac


cataaatccagaagcatctgcttctccttgctgcgtgtcccaagatttag


aacctctaaccattctctactacattggcaaaacacccaagattgaacag


ctttctaatatgattgtaaagtcttgcaaatgcagctaa


 


TGFB3 Mouse (nucleic acid sequence):


(SEQ ID NO: 223)


Atgaagatgcacttgcaaagggctctggtagtcctggccctgctgaactt


ggccacaatcagcctctctctgtccacttgcaccacgttggacttcggcc


acatcaagaagaagagggtggaagccattaggggacagatcttgagcaag


ctcaggctcaccagcccccctgagccatcggtgatgacccacgtccccta


tcaggtcctggcactttacaacagcacccgggagttgctggaagagatgc


acggggagagggaggaaggctgcactcaggagacctcggagtctgagtac


tatgccaaagagatccataaattcgacatgatccagggactggcggagca


caatgaactggccgtctgccccaaaggaattacctctaaggtttttcgtt


tcaatgtgtcctcagtggagaaaaatggaaccaatctgttccgggcagag


ttccgggtcttgcgggtgcccaaccccagctccaagcgcacagagcagag


aattgagctcttccagatacttcgaccggatgagcacatagccaagcagc


gctacataggtggcaagaatctgcccacaaggggcaccgctgaatggctg


tctttcgatgtcactgacactgtgcgcgagtggctgttgaggagagagtc


caacttgggtctggaaatcagcatccactgtccatgtcacacctttcagc


ccaatggagacatactggaaaatgttcatgaggtgatggaaatcaaattc


aaaggagtggacaatgaagatgaccatggccgtggagacctggggcgtct


caagaagcaaaaggatcaccacaacccacacctgatcctcatgatgatcc


ccccacaccgactggacagcccaggccagggcagtcagaggaagaagccc


accgagaacaacgaagacttcaacatcgtggccgtggccagcaacttcgc


gaccacggatctcgatgctgaccgcgggaagttgcccggcaagaagctgc


cgctggaggtgctcaaagagatggaagccaatgcccggaaagctggctgc


accaggggctgtctgatctgcctgtcccacatcaagtgcacgcccaagat


gaagaagttcatcccaggacgctgccacacctacgaaggcgacaaagagt


ccgcacagggcggcataggcgaggcgatcgtcgacattcctgagattcct


gggttcaaggacttggagcccatggagcagttcatcgcacaggtcgatct


gtgtgtggactgcacaactggctgcctcaaagggcttgccaacgtgcagt


gttctgacctgctcaagaagtggctgccgcaacgctgtgcgacctttgcc


agcaagatccagggccaggtggacaagatcaagggggccggtggtgacag


gaagaagagggccctggacaccaattactgcttccgcaacctggaggaga


actgctgtgtacgccccctttatattgacttccggcaggatctaggctgg


aaatgggtccacgaacctaagggttactatgccaacttctgctcaggccc


ttgcccatacctccgcagcgcagacacaacccatagcacggtgcttggac


tatacaacaccctgaacccagaggcgtctgcctcgccatgctgcgtcccc


caggacctggagcccctgaccatcttgtactatgtgggcagaacccccaa


ggtggagcagctgtccaacatggtggtgaagtcgtgtaagtgcagctga


 


TGFB3 Human (nucleic acid sequence):


(SEQ ID NO: 224)


Atgaagatgcacttgcaaagggctctggtggtcctggccctgctgaactt


tgccacggtcagcctctctctgtccacttgcaccaccttggacttcggcc


acatcaagaagaagagggtggaagccattaggggacagatcttgagcaag


ctcaggctcaccagcccccctgagccaacggtgatgacccacgtccccta


tcaggtcctggccctttacaacagcacccgggagctgctggaggagatgc


atggggagagggaggaaggctgcacccaggaaaacaccgagtcggaatac


tatgccaaagaaatccataaattcgacatgatccaggggctggcggagca


caacgaactggctgtctgccctaaaggaattacctccaaggttttccgct


tcaatgtgtcctcagtggagaaaaatagaaccaacctattccgagcagaa


ttccgggtcttgcgggtgcccaaccccagctctaagcggaatgagcagag


gatcgagctcttccagatccttcggccagatgagcacattgccaaacagc


gctatatcggtggcaagaatctgcccacacggggcactgccgagtggctg


tcctttgatgtcactgacactgtgcgtgagtggctgttgagaagagagtc


caacttaggtctagaaatcagcattcactgtccatgtcacacctttcagc


ccaatggagatatcctggaaaacattcacgaggtgatggaaatcaaattc


aaaggcgtggacaatgaggatgaccatggccgtggagatctggggcgcct


caagaagcagaaggatcaccacaaccctcatctaatcctcatgatgattc


ccccacaccggctcgacaacccgggccaggggggtcagaggaagaagccc


accgagaacaacgaagacttcaacatcgtggccgtggccagcaacttcgc


gaccacggatctcgatgctgaccgcgggaagttgcccggcaagaagctgc


cgctggaggtgctcaaagagatggaagccaatgcccggaaagctggctgc


accaggggctgtctgatctgcctgtcccacatcaagtgcacgcccaagat


gaagaagttcatcccaggacgctgccacacctacgaaggcgacaaagagt


ccgcacagggcggcataggcgaggcgatcgtcgacattcctgagattcct


gggttcaaggacttggagcccatggagcagttcatcgcacaggtcgatct


gtgtgtggactgcacaactggctgcctcaaagggcttgccaacgtgcagt


gttctgacctgctcaagaagtggctgccgcaacgctgtgcgacctttgcc


agcaagatccagggccaggtggacaagatcaagggggccggtggtgacag


gaagaagcgggctttggacaccaattactgcttccgcaacttggaggaga


actgctgtgtgcgccccctctacattgacttccgacaggatctgggctgg


aagtgggtccatgaacctaagggctactatgccaacttctgctcaggccc


ttgcccatacctccgcagtgcagacacaacccacagcacggtgctgggac


tgtacaacactctgaaccctgaagcatctgcctcgccttgctgcgtgccc


caggacctggagcccctgaccatcctgtactatgttgggaggacccccaa


agtggagcagctctccaacatggtggtgaagtcttgtaaatgtagctga


 


PDGFA Mouse (nucleic acid sequence):


(SEQ ID NO: 225)


Atgaggacctgggcttgcctgctgctcctcggctgcggatacctcgccca


tgccctggccgaggaagccgagataccccgggagttgatcgagcggctgg


ctcgaagtcagatccacagcatccgggacctccagcgactcttggagata


gactccgtaggggctgaggatgccttggagacaagtctgagagcccatgg


gtcccatgccattaaccatgtgcccgagaagcggcctgtgcccattcgca


ggaagcccaccgagaacaacgaagacttcaacatcgtggccgtggccagc


aacttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaa


gaagctgccgctggaggtgctcaaagagatggaagccaatgcccggaaag


ctggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacg


cccaagatgaagaagttcatcccaggacgctgccacacctacgaaggcga


caaagagtccgcacagggcggcataggcgaggcgatcgtcgacattcctg


agattcctgggttcaaggacttggagcccatggagcagttcatcgcacag


gtcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgccaa


cgtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcga


cctttgccagcaagatccagggccaggtggacaagatcaagggggccggt


ggtgaccgcaggaagagaagtattgaggaagccattcctgcagtttgcaa


gaccaggacggtcatttacgagatacctcggagccaggtggaccccacat


cggccaacttcctgatctggcccccatgtgtggaggtgaagcgctgcact


ggctgttgtaacaccagcagcgtcaagtgccagccttcacgggtccacca


ccgcagtgtcaaggtggccaaagtggagtatgtcaggaagaagccaaaat


tgaaagaggtccaggtgaggttagaggaacacctggagtgtgcatgtgcg


acctccaacctgaacccagaccatcgggaggaggagacagatgtgaggtg


a


 


PDGFA Human (nucleic acid sequence):


(SEQ ID NO: 226)


Atgaggaccttggcttgcctgctgctcctcggctgcggatacctcgccca


tgttctggccgaggaagccgagatcccccgcgaggtgatcgagaggctgg


cccgcagtcagatccacagcatccgggacctccagcgactcctggagata


gactccgtagggagtgaggattctttggacaccagcctgagagctcacgg


ggtccatgccactaagcatgtgcccgagaagcggcccctgcccattcgga


ggaagcccaccgagaacaacgaagacttcaacatcgtggccgtggccagc


aacttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaa


gaagctgccgctggaggtgctcaaagagatggaagccaatgcccggaaag


ctggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacg


cccaagatgaagaagttcatcccaggacgctgccacacctacgaaggcga


caaagagtccgcacagggcggcataggcgaggcgatcgtcgacattcctg


agattcctgggttcaaggacttggagcccatggagcagttcatcgcacag


gtcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgccaa


cgtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcga


cctttgccagcaagatccagggccaggtggacaagatcaagggggccggt


ggtgaccggaggaagagaagcatcgaggaagctgtccccgctgtctgcaa


gaccaggacggtcatttacgagattcctcggagtcaggtcgaccccacgt


ccgccaacttcctgatctggcccccgtgcgtggaggtgaaacgctgcacc


ggctgctgcaacacgagcagtgtcaagtgccagccctcccgcgtccacca


ccgcagcgtcaaggtggccaaggtggaatacgtcaggaagaagccaaaat


taaaagaagtccaggtgaggttagaggagcatttggagtgcgcctgcgcg


accacaagcctgaatccggattatcgggaagaggacacgggaaggcctag


ggagtcaggtaaaaaacggaaaagaaaaaggttaaaacccacctaa


 


BDNF Mouse (nucleic acid sequence):


(SEQ ID NO: 227)


Atgttccaccaggtgagaagagtgatgaccatccttttccttactatggt


tatttcatacttcggttgcatgaaggcggcgcccatgaaagaagtaaacg


tccacggacaaggcaacttggcctacccaggtgtgcggacccatgggact


ctggagagcgtgaatgggcccagggcaggttcgagaggtctgacgacgac


atcactggctgacacttttgagcacgtcatcgaagagctgctggatgagg


accagaaggttcggcccaacgaagaaaaccataaggacgcggacttgtac


acttcccgggtgatgctcagcagtcaagtgcctttggagcctcctctact


ctttctgctggaggaatacaaaaattacctggatgccgcaaacatgtcta


tgagggttcggcgcaagcccaccgagaacaacgaagacttcaacatcgtg


gccgtggccagcaacttcgcgaccacggatctcgatgctgaccgcgggaa


gttgcccggcaagaagctgccgctggaggtgctcaaagagatggaagcca


atgcccggaaagctggctgcaccaggggctgtctgatctgcctgtcccac


atcaagtgcacgcccaagatgaagaagttcatcccaggacgctgccacac


ctacgaaggcgacaaagagtccgcacagggcggcataggcgaggcgatcg


tcgacattcctgagattcctgggttcaaggacttggagcccatggagcag


ttcatcgcacaggtcgatctgtgtgtggactgcacaactggctgcctcaa


agggcttgccaacgtgcagtgttctgacctgctcaagaagtggctgccgc


aacgctgtgcgacctttgccagcaagatccagggccaggtggacaagatc


aagggggccggtggtgaccggcgccactccgaccctgcccgccgtgggga


gctgagcgtgtgtgacagtattagcgagtgggtcacagcggcagataaaa


agactgcagtggacatgtctggcgggacggtcacagtcctagagaaagtc


ccggtatccaaaggccaactgaagcagtatttctacgagaccaagtgtaa


tcccatgggttacaccaaggaaggctgcaggggcatagacaaaaggcact


ggaactcgcaatgccgaactacccaatcgtatgttcgggcccttactatg


gatagcaaaaagagaattggctggcgattcataaggatagacacttcctg


tgtatgtacactgaccattaaaaggggaagatag


 


BDNF Human (nucleic acid sequence):


(SEQ ID NO: 228)


Atgaccatccttttccttactatggttatttcatactttggttgcatgaa


ggctgcccccatgaaagaagcaaacatccgaggacaaggtggcttggcct


acccaggtgtgcggacccatgggactctggagagcgtgaatgggcccaag


gcaggttcaagaggcttgacatcattggctgacactttcgaacacgtgat


agaagagctgttggatgaggaccagaaagttcggcccaatgaagaaaaca


ataaggacgcagacttgtacacgtccagggtgatgctcagtagtcaagtg


cctttggagcctcctcttctctttctgctggaggaatacaaaaattacct


agatgctgcaaacatgtccatgagggtccggcgccactctgaccctgccc


gccgaaagcccaccgagaacaacgaagacttcaacatcgtggccgtggcc


agcaacttcgcgaccacggatctcgatgctgaccgcgggaagttgcccgg


caagaagctgccgctggaggtgctcaaagagatggaagccaatgcccgga


aagctggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgc


acgcccaagatgaagaagttcatcccaggacgctgccacacctacgaagg


cgacaaagagtccgcacagggcggcataggcgaggcgatcgtcgacattc


ctgagattcctgggttcaaggacttggagcccatggagcagttcatcgca


caggtcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgc


caacgtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtg


cgacctttgccagcaagatccagggccaggtggacaagatcaagggggcc


ggtggtgaccgccgaggggagctgagcgtgtgtgacagtattagtgagtg


ggtaacggcggcagacaaaaagactgcagtggacatgtcgggcgggacgg


tcacagtccttgaaaaggtccctgtatcaaaaggccaactgaagcaatac


ttctacgagaccaagtgcaatcccatgggttacacaaaagaaggctgcag


gggcatagacaaaaggcattggaactcccagtgccgaactacccagtcgt


acgtgcgggcccttaccatggatagcaaaaagagaattggctggcgattc


ataaggatagacacttcttgtgtatgtacattgaccattaaaaggggaag


atag


 


NGF Mouse (nucleic acid sequence):


(SEQ ID NO: 229)


Atgtccatgttgttctacactctgatcactgcgtttttgatcggcgtaca


ggcagaaccgtacacagatagcaatgtcccagaaggagactctgtccctg


aagcccactggactaaacttcagcattcccttgacacagccctccgcaga


gcccgcagtgcccctactgcaccaatagctgcccgagtgacagggcagac


ccgcaacatcactgtagaccccagactgtttaagaaacggagactccact


caccccgtgtgctgttcagcacccagcctccacccacctcttcagacact


ctggatctagacttccaggcccatggtacaatccctttcaacaggactca


ccggagcaagcgcaagcccaccgagaacaacgaagacttcaacatcgtgg


ccgtggccagcaacttcgcgaccacggatctcgatgctgaccgcgggaag


ttgcccggcaagaagctgccgctggaggtgctcaaagagatggaagccaa


tgcccggaaagctggctgcaccaggggctgtctgatctgcctgtcccaca


tcaagtgcacgcccaagatgaagaagttcatcccaggacgctgccacacc


tacgaaggcgacaaagagtccgcacagggcggcataggcgaggcgatcgt


cgacattcctgagattcctgggttcaaggacttggagcccatggagcagt


tcatcgcacaggtcgatctgtgtgtggactgcacaactggctgcctcaaa


gggcttgccaacgtgcagtgttctgacctgctcaagaagtggctgccgca


acgctgtgcgacctttgccagcaagatccagggccaggtggacaagatca


agggggccggtggtgacaagcgctcatccacccacccagtcttccacatg


ggggagttctcagtgtgtgacagtgtcagtgtgtgggttggagataagac


cacagccacagacatcaagggcaaggaggtgacagtgctggccgaggtga


acattaacaacagtgtattcagacagtacttttttgagaccaagtgccga


gcctccaatcctgttgagagtgggtgccggggcatcgactccaaacactg


gaactcatactgcaccacgactcacaccttcgtcaaggcgttgacaacag


atgagaagcaggctgcctggaggttcatccggatagacacagcctgtgtg


tgtgtgctcagcaggaaggctacaagaagaggctga


 


NGF Human (nucleic acid sequence);


(SEQ ID NO: 230)


Atgtccatgttgttctacactctgatcacagcttttctgatcggcataca


ggcggaaccacactcagagagcaatgtccctgcaggacacaccatccccc


aagcccactggactaaacttcagcattcccttgacactgcccttcgcaga


gcccgcagcgccccggcagcggcgatagctgcacgcgtggcggggcagac


ccgcaacattactgtggaccccaggctgtttaaaaagcggcgactccgtt


caccccgtgtgctgtttagcacccagcctccccgtgaagctgcagacact


caggatctggacttcgaggtcggtggtgctgcccccttcaacaggactca


caggagcaagcggaagcccaccgagaacaacgaagacttcaacatcgtgg


ccgtggccagcaacttcgcgaccacggatctcgatgctgaccgcgggaag


ttgcccggcaagaagctgccgctggaggtgctcaaagagatggaagccaa


tgcccggaaagctggctgcaccaggggctgtctgatctgcctgtcccaca


tcaagtgcacgcccaagatgaagaagttcatcccaggacgctgccacacc


tacgaaggcgacaaagagtccgcacagggcggcataggcgaggcgatcgt


cgacattcctgagattcctgggttcaaggacttggagcccatggagcagt


tcatcgcacaggtcgatctgtgtgtggactgcacaactggctgcctcaaa


gggcttgccaacgtgcagtgttctgacctgctcaagaagtggctgccgca


acgctgtgcgacctttgccagcaagatccagggccaggtggacaagatca


agggggccggtggtgacaagcggtcatcatcccatcccatcttccacagg


ggcgaattctcggtgtgtgacagtgtcagcgtgtgggttggggataagac


caccgccacagacatcaagggcaaggaggtgatggtgttgggagaggtga


acattaacaacagtgtattcaaacagtacttttttgagaccaagtgccgg


gacccaaatcccgttgacagcgggtgccggggcattgactcaaagcactg


gaactcatattgtaccacgactcacacctttgtcaaggcgctgaccatgg


atggcaagcaggctgcctggcggtttatccggatagatacggcctgtgtg


tgtgtgctcagcaggaaggctgtgagaagagcctga


 


Albumin Mouse (nucleic acid sequence):


(SEQ ID NO: 231)


Atgaagtgggtaacctttctcctcctcctcttcgtctccggctctgcttt


ttccaggggtgtgtttcgccgaaagcccaccgagaacaacgaagacttca


acatcgtggccgtggccagcaacttcgcgaccacggatctcgatgctgac


cgcgggaagttgcccggcaagaagctgccgctggaggtgctcaaagagat


ggaagccaatgcccggaaagctggctgcaccaggggctgtctgatctgcc


tgtcccacatcaagtgcacgcccaagatgaagaagttcatcccaggacgc


tgccacacctacgaaggcgacaaagagtccgcacagggcggcataggcga


ggcgatcgtcgacattcctgagattcctgggttcaaggacttggagccca


tggagcagttcatcgcacaggtcgatctgtgtgtggactgcacaactggc


tgcctcaaagggcttgccaacgtgcagtgttctgacctgctcaagaagtg


gctgccgcaacgctgtgcgacctttgccagcaagatccagggccaggtgg


acaagatcaagggggccggtggtgaccgccgagaagcacacaagagtgag


atcgcccatcggtataatgatttgggagaacaacatttcaaaggcctagt


cctgattgccttttcccagtatctccagaaatgctcatacgatgagcatg


ccaaattagtgcaggaagtaacagactttgcaaagacgtgtgttgccgat


gagtctgccgccaactgtgacaaatcccttcacactctttttggagataa


gttgtgtgccattccaaacctccgtgaaaactatggtgaactggctgact


gctgtacaaaacaagagcccgaaagaaacgaatgtttcctgcaacacaaa


gatgacaaccccagcctgccaccatttgaaaggccagaggctgaggccat


gtgcacctcctttaaggaaaacccaaccacctttatgggacactatttgc


atgaagttgccagaagacatccttatttctatgccccagaacttctttac


tatgctgagcagtacaatgagattctgacccagtgttgtgcagaggctga


caaggaaagctgcctgaccccgaagcttgatggtgtgaaggagaaagcat


tggtctcatctgtccgtcagagaatgaagtgctccagtatgcagaagttt


ggagagagagcttttaaagcatgggcagtagctcgtctgagccagacatt


ccccaatgctgactttgcagaaatcaccaaattggcaacagacctgacca


aagtcaacaaggagtgctgccatggtgacctgctggaatgcgcagatgac


agggcggaacttgccaagtacatgtgtgaaaaccaggcgactatctccag


caaactgcagacttgctgcgataaaccactgttgaagaaagcccactgtc


ttagtgaggtggagcatgacaccatgcctgctgatctgcctgccattgct


gctgattttgttgaggaccaggaagtgtgcaagaactatgctgaggccaa


ggatgtcttcctgggcacgttcttgtatgaatattcaagaagacaccctg


attactctgtatccctgttgctgagacttgctaagaaatatgaagccact


ctggaaaagtgctgcgctgaagccaatcctcccgcatgctacggcacagt


gcttgctgaatttcagcctcttgtagaagagcctaagaacttggtcaaaa


ccaactgtgatctttacgagaagcttggagaatatggattccaaaatgcc


attctagttcgctacacccagaaagcacctcaggtgtcaaccccaactct


cgtggaggctgcaagaaacctaggaagagtgggcaccaagtgttgtacac


ttcctgaagatcagagactgccttgtgtggaagactatctgtctgcaatc


ctgaaccgtgtgtgtctgctgcatgagaagaccccagtgagtgagcatgt


taccaagtgctgtagtggatccctggtggaaaggcggccatgcttctctg


ctctgacagttgatgaaacatatgtccccaaagagtttaaagctgagacc


ttcaccttccactctgatatctgcacacttccagagaaggagaagcagat


taagaaacaaacggctcttgctgagctggtgaagcacaagcccaaggcta


cagcggagcaactgaagactgtcatggatgactttgcacagttcctggat


acatgttgcaaggctgctgacaaggacacctgcttctcgactgagggtcc


aaaccttgtcactagatgcaaagacgccttagcctaa


 


Albumin Human (nucleic acid sequence):


(SEQ ID NO: 232)


atgaagtgggtaacctttatttcccttctttttctctttagctcggctta


ttccaggggtgtgtttcgtcgaaagcccaccgagaacaacgaagacttca


acatcgtggccgtggccagcaacttcgcgaccacggatctcgatgctgac


cgcgggaagttgcccggcaagaagctgccgctggaggtgctcaaagagat


ggaagccaatgcccggaaagctggctgcaccaggggctgtctgatctgcc


tgtcccacatcaagtgcacgcccaagatgaagaagttcatcccaggacgc


tgccacacctacgaaggcgacaaagagtccgcacagggcggcataggcga


ggcgatcgtcgacattcctgagattcctgggttcaaggacttggagccca


tggagcagttcatcgcacaggtcgatctgtgtgtggactgcacaactggc


tgcctcaaagggcttgccaacgtgcagtgttctgacctgctcaagaagtg


gctgccgcaacgctgtgcgacctttgccagcaagatccagggccaggtgg


acaagatcaagggggccggtggtgaccgtcgagatgcacacaagagtgag


gttgctcatcggtttaaagatttgggagaagaaaatttcaaagccttggt


gttgattgcctttgctcagtatcttcagcagtgtccatttgaagatcatg


taaaattagtgaatgaagtaactgaatttgcaaaaacatgtgttgctgat


gagtcagctgaaaattgtgacaaatcacttcataccctttttggagacaa


attatgcacagttgcaactcttcgtgaaacctatggtgaaatggctgact


gctgtgcaaaacaagaacctgagagaaatgaatgcttcttgcaacacaaa


gatgacaacccaaacctcccccgattggtgagaccagaggttgatgtgat


gtgcactgcttttcatgacaatgaagagacatttttgaaaaaatacttat


atgaaattgccagaagacatccttacttttatgccccggaactccttttc


tttgctaaaaggtataaagctgcttttacagaatgttgccaagctgctga


taaagctgcctgcctgttgccaaagctcgatgaacttcgggatgaaggga


aggcttcgtctgccaaacagagactcaagtgtgccagtctccaaaaattt


ggagaaagagctttcaaagcatgggcagtagctcgcctgagccagagatt


tcccaaagctgagtttgcagaagtttccaagttagtgacagatcttacca


aagtccacacggaatgctgccatggagatctgcttgaatgtgctgatgac


agggcggaccttgccaagtatatctgtgaaaatcaagattcgatctccag


taaactgaaggaatgctgtgaaaaacctctgttggaaaaatcccactgca


ttgccgaagtggaaaatgatgagatgcctgctgacttgccttcattagct


gctgattttgttgaaagtaaggatgtttgcaaaaactatgctgaggcaaa


ggatgtcttcctgggcatgtttttgtatgaatatgcaagaaggcatcctg


attactctgtcgtgctgctgctgagacttgccaagacatatgaaaccact


ctagagaagtgctgtgccgctgcagatcctcatgaatgctatgccaaagt


gttcgatgaatttaaacctcttgtggaagagcctcagaatttaatcaaac


aaaattgtgagctttttgagcagcttggagagtacaaattccagaatgcg


ctattagttcgttacaccaagaaagtaccccaagtgtcaactccaactct


tgtagaggtctcaagaaacctaggaaaagtgggcagcaaatgttgtaaac


atcctgaagcaaaaagaatgccctgtgcagaagactatctatccgtggtc


ctgaaccagttatgtgtgttgcatgagaaaacgccagtaagtgacagagt


caccaaatgctgcacagaatccttggtgaacaggcgaccatgcttttcag


ctctggaagtcgatgaaacatacgttcccaaagagtttaatgctgaaaca


ttcaccttccatgcagatatatgcacactttctgagaaggagagacaaat


caagaaacaaactgcacttgttgagctcgtgaaacacaagcccaaggcaa


caaaagagcaactgaaagctgttatggatgatttcgcagcttttgtagag


aagtgctgcaaggctgacgataaggagacctgctttgccgaggagggtaa


aaaacttgttgctgcaagtcaagctgccttaggcttataa


 


Calcitonin Mouse (nucleic acid sequence):


(SEQ ID NO: 233)


Atgggcttcctgaagttctcccctttcctggttgtcagcatcttgctcct


gtaccaggcatgcagcctccaggcagtgcctttgaggtcaatcttggaaa


gcagcccaggcatggccactctcagtgaagaagaagttcgcctgctggct


gcactggtgcaggactatatgcagatgaaagccagggagctggagcagga


ggaagagcaggaggctgagggctctagcttggacagccccagatctaagc


ggaagcccaccgagaacaacgaagacttcaacatcgtggccgtggccagc


aacttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaa


gaagctgccgctggaggtgctcaaagagatggaagccaatgcccggaaag


ctggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacg


cccaagatgaagaagttcatcccaggacgctgccacacctacgaaggcga


caaagagtccgcacagggcggcataggcgaggcgatcgtcgacattcctg


agattcctgggttcaaggacttggagcccatggagcagttcatcgcacag


gtcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgccaa


cgtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcga


cctttgccagcaagatccagggccaggtggacaagatcaagggggccggt


ggtgacaagcggtgtgggaatctgagtacctgcatgctgggcacgtacac


acaagacctcaacaagtttcacaccttcccccaaacttcaattggggttg


aagcacctggcaagaaaagggatgtggccaaggacttggagacaaaccac


caatcccattttggcaactaa


 


Calcitonin Human (nucleic acid sequence):


(SEQ ID NO: 234)


Atgggcttccaaaagttctcccccttcctggctctcagcatcttggtcct


gttgcaggcaggcagcctccatgcagcaccattcaggtctgccctggaga


gcagcccagcagacccggccacgctcagtgaggacgaagcgcgcctcctg


ctggctgcactggtgcaggactatgtgcagatgaaggccagtgagctgga


gcaggagcaagagagagagggctccagcctggacagccccagatctaagc


ggaagcccaccgagaacaacgaagacttcaacatcgtggccgtggccagc


aacttcgcgaccacggatctcgatgctgaccgcgggaagttgcccggcaa


gaagctgccgctggaggtgctcaaagagatggaagccaatgcccggaaag


ctggctgcaccaggggctgtctgatctgcctgtcccacatcaagtgcacg


cccaagatgaagaagttcatcccaggacgctgccacacctacgaaggcga


caaagagtccgcacagggcggcataggcgaggcgatcgtcgacattcctg


agattcctgggttcaaggacttggagcccatggagcagttcatcgcacag


gtcgatctgtgtgtggactgcacaactggctgcctcaaagggcttgccaa


cgtgcagtgttctgacctgctcaagaagtggctgccgcaacgctgtgcga


cctttgccagcaagatccagggccaggtggacaagatcaagggggccggt


ggtgacaagcggtgcggtaatctgagtacttgcatgctgggcacatacac


gcaggacttcaacaagtttcacacgttcccccaaactgcaattggggttg


gagcacctggaaagaaaagggatatgtccagcgacttggagagagaccat


cgccctcatgttagcatgccccagaatgccaactaa









Further exemplary luciferase fusion proteins (their nucleic acid sequences) include, but are not limited to, the sequences below. The portions italicized correspond to inserted nucleotides encoding Cypridina luciferase without its signal peptide. Underline indicates the putative cleavage sites.








Proamylin-luciferase Mouse (nucleic acid sequence):



(SEQ ID NO: 235)



Atgatgtgcatctccaaactgccagctgtcctcctcatcctctctgtggcactgaaccacttgagagctacacctgtcagaagtggtagca



 


acccctcagatggacaaacggaagtgcaacacggccacgtgtgccacacaacgcctggcaaactttttggttcgttccagcaacaacctt


 


ggtccagtcctcccaccaaccaacgtgggatcgaatacatatggcaagaggaatgcgTactgcgccactgttcattgccaggactgtc


 


cttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgc


 


acgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcag


 


agtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaa


 


ccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactg


 


gaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaa


 


cccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtga


 


tcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaa


 


aatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccact


 


ctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctacta


 


ctacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcg


 


ctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtcc


 


ctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaa


 


gtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaacagattctggttggaggagaagccg


 


tgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggt


 


ggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaacc


 


aggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacct


 


gaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtc


 


gaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataa


 


agcatggagacaccctagaagtaccagatgaatgcaaaaagaggaatgcggcaggggatccaaatagggaatccttggatttctta


 


ctcgtttaa


 


Proamylin-luciferase Human (nucleic acid sequence):


(SEQ ID NO: 236)



Atgggcatcctgaagctgcaagtatttctcattgtgctctctgttgcattgaaccatctgaaagctacacccattgaaagtcatcaggt



 


ggaaaagcggaaatgcaacactgccacatgtgcaacgcagcgcctggcaaatttttagttcattccagcaacaactttggtgccat


 


tctctcatctaccaacgtgggatccaatacatggcaagaggaatgcaTactgcgccactgttcattgccaggactgtccttacga


 


acctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgaga


 


gacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtaga


 


ggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagg


 


gcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtg


 


gctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgta


 


caccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgac


 


atcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatga


 


tggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatgg


 


aaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacac


 


catctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcc


 


cgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaa


 


ggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagag


 


aaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgt


 


cccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaa


 


gttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatt


 


tcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagct


 


gaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacga


 


tgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatg


 


gagacaccctagaagtaccagatgaatgcaaaaagaggaatgcggtagaggttttaaagagagagccactgaattacttgccccttt


 


ag


 


Proinsulin-luciferase Mouse (nucleic acid sequence):


(SEQ ID NO: 237)



Atggccctgtggatgcgcttcctgcccctgctggccctgctcttcctctgggagtcccaccccacccaggcttttgtcaagcagcaccttt



 


gtggttcccacctggtggaggctctctacctggtgtgtggggagcgtggcttcttctacacacccatgtcccgccgtgaagtggaggac


 


ccacaagtggcacaactggagctgggtggaggcccgggagcaggtgaccttcagaccttggcactggaggtggcccagcagaaga


 


ggTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaag


 


gagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacat


 


gttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttcc


 


aggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaa


 


ccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatca


 


ctgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttca


 


acatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaac


 


aaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcag


 


cctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtaca


 


aggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcct


 


cacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatac


 


atttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaa


 


ggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttga


 


tggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacat


 


actgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatg


 


gtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaac


 


ccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcaga


 


aatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccac


 


gcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaagcgtggcattgta


 


gatcagtgctgcaccagcatctgctccctctaccagctggagaactactgcaactag


 


Proinsulin-luciferase Human (nucleic acid sequence) (nucleic acid sequence):


(SEQ ID NO: 238)



Atggccctgtggatgcgcctcctgcccctgctggcgctgctggccctctggggacctgacccagccgcagcctttgtgaaccaacacc



 


tgtgcggctcacacctggtggaagctctctacctagtgtgcggggaacgaggcttcttctacacacccaagacccgccgggaggcaga


 


ggacctgcaggtggggcaggtggagctgggcgggggccctggtgcaggcagcctgcagcccttggccctggaggggtccctgca


 


gaagaggTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaa


 


agaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaa


 


aacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattcca


 


gttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggat


 


ggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatccc


 


atcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccagg


 


cttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagc


 


aaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctat


 


tcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccg


 


tacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcg


 


agcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctac


 


gatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggat


 


gtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcat


 


tgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatgg


 


tgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccat


 


tcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgaccc


 


ccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttga


 


tcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtg


 


accacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaagcgtggc


 


attgtggaacaatgctgtaccagcatctgctccctctaccagctggagaactactgcaactag


 


Proglucagon (includes GRPP, glucagon, GLP-1, GLP-2) Mouse (nucleic acid sequence):


(SEQ ID NO: 239)



Atgaagaccatttactttgtggctggattgcttataatgctggtgcaaggcagctggcagcacgcccttcaagacacagaggagaaccc



 


cagatcattcccagcttcccagacagaagcgcatgaggaccctgatgagatgaatgaagacaaacgccactcacagggcacattcac


 


cagcgactacagcaaatacctggactcccgccgtgcccaagattttgtgcagtggttgatgaacaccaagaggaaccggaacaacatt


 


gccaaacgtcatgatgaatttgagaggcatgctgaagggacctttaccagtgatgtgagttcttacttggagggccaggcagcaaagga


 


attcattgcttggctggtgaaaggccgaggaaggcgagacttcccagaagaagtcgccattgccgaggaactcggccgaaagagaag


 


aTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaagg


 


agaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgt


 


tgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccag


 


gaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaacc


 


aagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactg


 


taaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaac


 


atcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaa


 


aggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcct


 


aagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaag


 


gacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctca


 


cacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatt


 


tgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggt


 


ttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatgg


 


aaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatact


 


gactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggta


 


agacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaaccca


 


ccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaat


 


gtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgca


 


tgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaagcgcggccgcaggca


 


cgctgatggctccttctctgacgagatgagcaccattctggataatcttgccaccagggacttcatcaactggctgattcaaaccaagatc


 


actgacaagaaatag


 


Proglucagon (includes GRPP, glucagon, GLP-1, GLP-2) Human (nucleic acid sequence)


(nucleic acid sequence):


(SEQ ID NO: 240)



atgaaaagcatttactttgtggctggattatttgtaatgctggtacaaggcagctggcaacgttcccttcaagacacagaggagaaatcca



 


gatcattctcagcttcccaggcagacccactcagtgatcctgatcagatgaacgaggacaagcgccattcacagggcacattcaccagt


 


gactacagcaagtatctggactccaggcgtgcccaagattttgtgcagtggttgatgaataccaagaggaacaggaataacattgccaa


 


acgtcacgatgaatttgagagacatgctgaagggacctttaccagtgatgtaagttcttatttggaaggccaagctgccaaggaattcatt


 


gcttggctggtgaaaggccgaggaaggcgagatttcccagaagaggtcgccattgttgaagaacttggccgaaagagaagaTactg


 


cgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatg


 


tattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccga


 


atgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacct


 


ggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaaggg


 


ggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaac


 


ggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcacc


 


gtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaat


 


gatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatc


 


aaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagc


 


tgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtg


 


ctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgaca


 


aagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcac


 


acaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaa


 


cagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgact


 


acagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaaga


 


cttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccg


 


ggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgta


 


acgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgg


 


gagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaagcgcggccgcagacatgc


 


tgatggttctttctctgatgagatgaacaccattcttgataatcttgccgccagggactttataaactggttgattcagaccaaaatcactgac


 


aggaaataa


 


Peptide YY Mouse (nucleic acid sequence):


(SEQ ID NO: 241)



atggtggcggtgcgcaggccttggcccgtcacggtcgcaatgctgctaatcctgctcgcctgtctgggagccctggtggacgcctac



 


cctgccaaaccagaggctcccggcgaagacgcctccccggaggagctgagccgctactacgcctccctgcgccactacctcaac


 


ctggtcacccggcagcggtatggaaaaagaTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaaca


 


cagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatgga


 


ctgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttag


 


aacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtg


 


tccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgac


 


atcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcac


 


catcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctg


 


taagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcac


 


ttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgca


 


tactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgccc


 


gctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacct


 


gcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgcc


 


gactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaa


 


caatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaac


 


acttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgc


 


tcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgc


 


tgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtct


 


cttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcct


 


gaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtac


 


cagatgaatgcaaaaagaggagggatgtccccgcagctctgttctccaaactgctcttcacagacgacagcgacagcgagaacctcc


 


ccttcaggccagaaggtttggaccagtggtga


 


Peptide YY Human (nucleic acid sequence):


(SEQ ID NO: 242)



Atggtgttcgtgcgcaggccgtggcccgccttgaccacagtgcttctggccctgctcgtctgcctaggggcgctggtcgacgcctacc



 


ccatcaaacccgaggctcccggcgaagacgcctcgccggaggagctgaaccgctactacgcctccctgcgccactacctcaacctg


 


gtcacccggcagcggtatgggaaaagaTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagtt


 


ccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtg


 


tgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaaca


 


ttctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccat


 


caccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcg


 


ctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatc


 


gctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaag


 


aatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcag


 


atccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactg


 


caaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgt


 


atgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttt


 


tgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactg


 


tttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatc


 


gactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttc


 


catctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtc


 


gtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaa


 


ggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcg


 


ccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgagg


 


ggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagat


 


gaatgcaaaaagaggagggacggcccggacacgcttctttccaaaacgttcttccccgacggcgaggaccgccccgtcaggtcgcg


 


gtcggagggcccagacctgtggtga


 


Neuropeptide Y Mouse (nucleic acid sequence):


(SEQ ID NO: 243)



Atgctaggtaacaagcgaatggggctgtgtggactgaccctcgctctatctctgctcgtgtgtttgggcattctggctgaggggtaccc



 


ctccaagccggacaatccgggcgaggacgcgccagcagaggacatggccagatactactccgctctgcgacactacatcaatct


 


catcaccagacagagatatggcaagagaTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacaca


 


gttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggact


 


gtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttaga


 


acattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgt


 


ccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgac


 


atcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcac


 


catcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctg


 


taagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcac


 


ttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgca


 


tactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgccc


 


gctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacct


 


gcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgcc


 


gactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaa


 


caatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaac


 


acttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgc


 


tcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgc


 


tgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtct


 


cttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcct


 


gaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtac


 


cagatgaatgcaaaaagaggaggtccagccctgagacactgatttcagacctcttaatgaaggaaagcacagaaaacgcccccaga


 


acaaggcttgaagacccttccatgtggtga


 


Neuropeptide Y Human (nucleic acid sequence):


(SEQ ID NO: 244)



Atgctaggtaacaagcgactggggctgtccggactgaccctcgccctgtccctgctcgtgtgcctgggtgcgctggccgaggcgtac



 


ccctccaagccggacaacccgggcgaggacgcaccagcggaggacatggccagatactactcggcgctgcgacactacatcaacc


 


tcatcaccaggcagagatatggaaaacgaTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacag


 


ttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgt


 


gtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaac


 


attctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtcc


 


atcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatc


 


gctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccat


 


cgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaa


 


gaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttca


 


gatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatact


 


gcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgct


 


gtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcg


 


ttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgac


 


tgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaa


 


tcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacactt


 


ccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgt


 


cgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctga


 


aggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttc


 


gccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgag


 


gggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccag


 


atgaatgcaaaaagaggaggtccagcccagagacactgatttcagacctcttgatgagagaaagcacagaaaatgttcccagaactc


 


ggcttgaagaccctgcaatgtggtga


 


Pancreatic polypeptide Mouse (nucleic acid sequence):


(SEQ ID NO: 245)



Atggccgtcgcatactgctgcctctccctgtttctcgtatccacttgggtggctctgctgctgcagcccctgcaggggacctggggagcc



 


cccctggagccaatgtacccaggcgactatgcgacacctgagcagatggcacaatatgaaactcagctccgcagatacatcaacacac


 


tgaccaggcctaggtatgggaagagaTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttc


 


caacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgt


 


gaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacat


 


tctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccat


 


caccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcg


 


ctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatc


 


gctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaag


 


aatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcag


 


atccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactg


 


caaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgt


 


atgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttt


 


tgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactg


 


tttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatc


 


gactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttc


 


catctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtc


 


gtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaa


 


ggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcg


 


ccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgagg


 


ggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagat


 


gaatgcaaaaagaggagggccgaggaggagaacacaggtggacttcctggagtgcagctctccccctgcaccagccccccagttg


 


gcttgattccctgctctgcgccctggagctga


 


Pancreatic polypeptide Human (nucleic acid sequence):


(SEQ ID NO: 246)



Atggctgccgcacgcctctgcctctccctgctgctcctgtccacctgcgtggctctgttactacagccactgctgggtgcccagggag



 


ccccactggagccagtgtacccaggggacaatgccacaccagagcagatggcccagtatgcagctgatctccgtagatacatca


 


acatgctgaccaggcctaggtatgggaaaagaTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaac


 


acagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatgg


 


actgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggttta


 


gaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggt


 


gtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattga


 


catcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtca


 


ccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatct


 


gtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttca


 


cttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgc


 


atactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcc


 


cgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacct


 


gcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgcc


 


gactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaa


 


caatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaac


 


acttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgc


 


tcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgc


 


tgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtct


 


cttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcct


 


gaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtac


 


cagatgaatgcaaaaagaggaggcacaaagaggacacgctggccttctcggagtgggggtccccgcatgctgctgtccccaggga


 


gctcagcccgctggacttataa


 


Somatostatin Mouse (nucleic acid sequence):


(SEQ ID NO: 247)



Atgctgtcctgccgtctccagtgcgccctggctgcgctctgcatcgtcctggctttgggcggtgtcaccggcgcgccctcggacccca



 


gactccgtcagtttctgcagaagtctctggcggctgccaccgggaaacaggaactggccaagtacttcttggcagagctgcgcaaaTa


 


ctgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggaga


 


atgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgcc


 


gaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaa


 


cctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaag


 


ggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaa


 


acggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatca


 


ccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaagg


 


aatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaag


 


atcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggac


 


agctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacac


 


gtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttga


 


caaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttc


 


acacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaa


 


aacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactga


 


ctacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaag


 


acttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccacc


 


gggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgt


 


aacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatg


 


ggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaacgcaaactgtccgagcccaa


 


ccagacagagaatgatgccctggagcccgaggatttgccccaggcagctgagcaggacgagatgaggctggagctgcagaggtct


 


gccaactcgaacccagcaatggcaccccgggaacgcaaagctggctgcaagaacttcttctggaagacattcacatcctgttag


 


Somatostatin Human (nucleic acid sequence):


(SEQ ID NO: 248)



Atgctgtcctgccgcctccagtgcgcgctggctgcgctgtccatcgtcctggccctgggctgtgtcaccggcgctccctcggacccca



 


gactccgtcagtttctgcagaagtccctggctgctgccgcggggaagcaggaactggccaagtacttcttggcagagctgcgcaaaTa


 


ctgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggaga


 


atgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgcc


 


gaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaa


 


cctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaag


 


ggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaa


 


acggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatca


 


ccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaagg


 


aatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaag


 


atcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggac


 


agctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacac


 


gtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttga


 


caaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttc


 


acacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaa


 


aacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactga


 


ctacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaag


 


acttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccacc


 


gggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgt


 


aacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatg


 


ggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaacgcaaactgtctgaacccaa


 


ccagacggagaatgatgccctggaacctgaagatctgtcccaggctgctgagcaggatgaaatgaggcttgagctgcagagatctgct


 


aactcaaacccggctatggcaccccgagaacgcaaagctggctgcaagaatttcttctggaagactttcacatcctgttag


 


GHRH Mouse (nucleic acid sequence):


(SEQ ID NO: 249)



Atgctgctctgggtgctctttgtgatcctcatcctcaccagtggctcccactgctcactgcccccctcacctcccttcaggatgcagcgac



 


acgtagatgccatcttcaccaccaactacaggaaactcctgagccagctgtatgcccggaaagtgatccaggacatcatgaacaagca


 


aggggagaggatccaggaacaaagggccaggctcagccgccaggaagacagcatgtggacagaggacaagcagatgaccctgg


 


agagcatccggcggTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtg


 


aagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaac


 


caggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaag


 


agattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggaga


 


acctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactg


 


agaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgag


 


atgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgcccca


 


gacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaac


 


aactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtctt


 


ctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtgga


 


gacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggaca


 


tactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaac


 


acttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagt


 


agaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactg


 


gcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatatt


 


agagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctg


 


tgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaa


 


agtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaaca


 


gggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaa


 


acggcggttgcagggattcccaaggatgaagccttcagcggacgcttga


 


GHRH Human (nucleic acid sequence):


(SEQ ID NO: 250)



Atgccactctgggtgttcttctttgtgatcctcaccctcagcaacagctcccactgctccccacctccccctttgaccctcaggatgcggc



 


ggtatgcagatgccatcttcaccaacagctaccggaaggtgctgggccagctgtccgcccgcaagctgctccaggacatcatgagca


 


ggcagcagggagagagcaaccaagagcgaggagcaagggcacggcttggtcgtcaggtagacagcatgtgggcagaacaaaag


 


caaatggaattggagagcatcctggtggccctgcggcggTactgcgccactgttcattgccaggactgtccttacgaacctgatccac


 


caaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatc


 


agatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcagga


 


tggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactg


 


gaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagaca


 


tcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcg


 


aggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggagga


 


agatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacag


 


acttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatga


 


cgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgc


 


cttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactaga


 


ggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttat


 


ggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaat


 


caggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagct


 


ctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaa


 


gcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgatt


 


cttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctg


 


caatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacga


 


gtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacacccta


 


gaagtaccagatgaatgcaaacggcggctgcagaagcacaggaactcccagggatga


 


POMC (ACTH, MSH) Mouse (nucleic acid sequence):


(SEQ ID NO: 251)



Atgccgagattctgctacagtcgctcaggggccctgttgctggccctcctgcttcagacctccatagatgtgtggagctggtgcctgga



 


gagcagccagtgccaggacctcaccacggagagcaacctgctggcttgcatccgggcttgcaaactcgacctctcgctggagacgcc


 


cgtgtttcctggcaacggagatgaacagcccctgactgaaaacccccggaagtacgtcatgggtcacttccgctgggaccgcttcggc


 


cccaggaacagcagcagtgctggcagcgcggcgcagaggcgtgcggaggaagaggcggtgtggggagatggcagtccagagcc


 


gagtccacgcgagggcaagcgcTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaa


 


cttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaa


 


aataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattcta


 


tggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcacc


 


ctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaa


 


gctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgtt


 


gttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcg


 


ccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatcca


 


gaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaa


 


ggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgg


 


gtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtct


 


ggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttct


 


ggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgac


 


tgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatc


 


tactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtac


 


atattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggag


 


cctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccgg


 


tcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggac


 


aacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaat


 


gcaaaaagcgctcctactccatggagcacttccgctggggcaagccggtgggcaagaaacggcgcccggtgaaggtgtaccccaa


 


cgttgctgagaacgagtcggcggaggcctttcccctagagttcaagagggagctggaaggcgagcggccattaggcttggagcaggt


 


cctggagtccgacgcggagaaggacgacgggccctaccgggtggagcacttccgctggagcaacccgcccaaggacaagcgttac


 


ggtggcttcatgacctccgagaagagccagacgcccctggtgacgctcttcaagaacgccatcatcaagaacgcgcacaagaaggg


 


ccagtga


 


POMC (ACTH, MSH) Human (nucleic acid sequence):


(SEQ ID NO: 252)



Atgccgagatcgtgctgcagccgctcgggggccctgttgctggccttgctgcttcaggcctccatggaagtgcgtggctggtgcctgg



 


agagcagccagtgtcaggacctcaccacggaaagcaacctgctggagtgcatccgggcctgcaagcccgacctctcggccgagact


 


cccatgttcccgggaaatggcgacgagcagcctctgaccgagaacccccggaagtacgtcatgggccacttccgctgggaccgattc


 


ggccgccgcaacagcagcagcagcggcagcagcggcgcagggcagaagcgcgaggacgtctcagcgggcgaagactgcggcc


 


cgctgcctgagggcggccccgagccccgcagcgatggtgccaagccgggcccgcgcgagggcaagcgcTactgcgccactgttc


 


attgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagca


 


gctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtat


 


gtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgt


 


gttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctga


 


ccaagacaagactggagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctga


 


ccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttct


 


tcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcct


 


ctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtt


 


tgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccc


 


catcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactac


 


agggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagatac


 


caattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttg


 


actcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttg


 


gaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatccta


 


cctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgc


 


ggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaa


 


gaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaa


 


gcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaa


 


gaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaagcgctcctactccatggagcacttccgctggg


 


gcaagccggtgggcaagaagcggcgcccagtgaaggtgtaccctaacggcgccgaggacgagtcggccgaggccttccccctgg


 


agttcaagagggagctgactggccagcgactccgggagggagatggccccgacggccctgccgatgacggcgcaggggcccagg


 


ccgacctggagcacagcctgctggtggcggccgagaagaaggacgagggcccctacaggatggagcacttccgctggggcagcc


 


cgcccaaggacaagcgctacggcggtttcatgacctccgagaagagccagacgcccctggtgacgctgttcaaaaacgccatcatca


 


agaacgcctacaagaagggcgagtga


 


Oxytocin Mouse (nucleic acid sequence):


(SEQ ID NO: 253)



Atggcctgccccagtctcgcttgctgcctgcttggcttactggctctgacctcggcctgctacatccagaactgccccctgggcggcaa



 


gaggTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaaga


 


aggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaac


 


atgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttc


 


caggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatgga


 


accaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatc


 


actgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttc


 


aacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaa


 


caaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattca


 


gcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtac


 


aaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagc


 


ctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgat


 


acatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtg


 


aaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgtt


 


gatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtga


 


catactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcg


 


atggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgaccccc


 


aacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatc


 


agaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgac


 


cacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaagcgcgctgt


 


gctggacctggatatgcgcaagtgtctcccctgcggcccgggcggcaaaggacgctgcttcggaccaagcatctgctgcgcggacg


 


agctgggctgcttcgtgggcaccgccgaggcgctgcgctgccaggaggagaactacctgccttcgccctgccagtctggccagaag


 


ccctgcgggagcggaggccgctgcgccgccacaggcatctgctgcagcccggatggctgccgcacagaccccgcctgcgaccctg


 


agtctgccttctcggagcgctga


 


Oxytocin Human (nucleic acid sequence):


(SEQ ID NO: 254)



Atggccggccccagcctcgcttgctgtctgctcggcctcctggcgctgacctccgcctgctacatccagaactgccccctgggaggca



 


agaggTactgcgccactgttcattgccaggactgtccttacgaacctgatccacaccaaacacagttccaacttcctgtgaagctaaag


 


aaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaa


 


catgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagtt


 


ccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatgg


 


aaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccat


 


cactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggctt


 


caacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaa


 


acaaaggaatgatctctggcctgtgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattc


 


agcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgta


 


caaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgag


 


cctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacga


 


tacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtg


 


aaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgtt


 


gatggaaaacagattctggttggaggagaagcctgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtga


 


catactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcg


 


atggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgaccccc


 


aacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatc


 


agaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgac


 


cacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaagcgcgccgc


 


gccggacctcgacgtgcgcaagtgcctcccctgcggccccgggggcaaaggccgctgcttcgggcccaatatctgctgcgcggaag


 


agctgggctgcttcgtgggcaccgccgaagcgctgcgctgccaggaggagaactacctgccgtcgccctgccagtccggccagaag


 


gcgtgcgggagcgggggccgctgcgcggtcttgggcctctgctgcagcccggacggctgccacgccgaccctgcctgcgacgcgg


 


aagccaccttctcccagcgctga


 


Vasopressin-Neurophysin-2 Mouse (nucleic acid sequence):


(SEQ ID NO: 255)



atgctcgccaggatgctcaacactacgctctccgcttgtttcctgagcctgctggccttctcctccgcctgctacttccagaactgcccaag



 


aggcggcaagaggTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtga


 


agctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaacc


 


aggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaaga


 


gattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaa


 


cctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactga


 


gaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagat


 


gccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccaga


 


cacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaa


 


ctcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttct


 


ggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggag


 


acgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacat


 


actttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaaca


 


cttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagta


 


gaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggc


 


aagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattag


 


agatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtg


 


atctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaa


 


gtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacag


 


ggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaa


 


aagcgcgccatctctgacatggagctgagacagtgtctcccctgcggcccgggcggcaaaggacgctgcttcggaccaagcatctgc


 


tgcgcggacgagctgggctgcttcgtgggcaccgccgaggcgctgcgctgccaggaggagaactacctgccctcgccctgccagtc


 


cggccagaagccctgcgggagcgggggccgctgcgccgccgtgggcatctgctgcagcgacgagagctgcgtggccgagcccg


 


agtgccacgacggttttttccgcctcacccgcgctcgggagccaagcaacgccacacagctggacggccctgctcgggcgctgctgc


 


taaggctggtacagctggctgggacacgggagtccgtggattctgccaagccccgggtctactga


 


Vasopressin-Neurophysin-2 Human (nucleic acid sequence):


(SEQ ID NO: 256)



Atgcctgacaccatgctgcccgcctgcttcctcggcctactggccttctcctccgcgtgctacttccagaactgcccgaggggcggcaa



 


gaggTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaaga


 


aggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaac


 


atgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttc


 


caggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatgga


 


accaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatc


 


actgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttc


 


aacataccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaa


 


caaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattca


 


gcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtac


 


aaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagc


 


ctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgat


 


acatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtg


 


aaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgtt


 


gatggaaaacagattctggttggaggagaagccgtgtccgtcccgttacagctctcagaacacttccatctactggcaagatggtga


 


catactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcg


 


atggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgaccccc


 


aacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatc


 


agaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgac


 


cacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaagcgcgccat


 


gtccgacctggagctgagacagtgcctcccctgcggccccgggggcaaaggccgctgcttcgggcccagcatctgctgcgcggacg


 


agctgggctgcttcgtgggcacggctgaggcgctgcgctgccaggaggagaactacctgccgtcgccctgccagtccggccagaag


 


gcgtgcgggagcgggggccgctgcgccgccttcggcgtttgctgcaacgacgagagctgcgtgaccgagcccgagtgccgcgag


 


ggctttcaccgccgcgcccgcgccagcgaccggagcaacgccacgcagctggacgggccggccggggccttgctgctgcggctg


 


gtgcagctggccggggcgcccgagcccttcgagcccgcccagcccgacgcctactga


 


Gonadotropin-releasing hormone (GnRH) Mouse (nucleic acid sequence):


(SEQ ID NO: 257)



Atgatcctcaaactgatggccggcattctactgctgactgtgtgtttggaaggctgctccagccagcactggtcctatgggttgcgccc



 


tgggggaaagagaTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtga


 


agctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaacc


 


aggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaaga


 


gattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaa


 


cctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactga


 


gaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagat


 


gccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccaga


 


cacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaa


 


ctcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttct


 


ggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggag


 


acgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacat


 


actttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaaca


 


cttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagta


 


gaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggc


 


aagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattag


 


agatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtg


 


atctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaa


 


gtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacag


 


ggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaa


 


aagcgcaacactgaacacttggttgagtctttccaagagatgggcaaggaggtggatcaaatggcagaaccccagcacttcgaatgta


 


ctgtccactggccccgttcacccctcagggatctgcgaggagctctggaaagtctgattgaagaggaagccaggcagaagaagatgta


 


g


 


Gonadotropin-releasing hormone (GnRH) Human (nucleic acid sequence):


(SEQ ID NO: 258)



Atgaagccaattcaaaaactcctagctggccttattctactgacttggtgcgtggaaggctgctccagccagcactggtcctatggac



 


tgcgccctggaggaaagagaTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaact


 


tcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaa


 


ataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctat


 


ggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcacc


 


ctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaa


 


gctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgtt


 


gttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcg


 


ccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatcca


 


gaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaa


 


ggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgg


 


gtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtct


 


ggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttct


 


ggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgac


 


tgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatc


 


tactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtac


 


atattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggag


 


cctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccgg


 


tcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggac


 


aacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaat


 


gcaaaaagcgcgatgccgaaaatttgattgattctttccaagagatagtcaaagaggttggtcaactggcagaaacccaacgcttcgaa


 


tgcaccacgcaccagccacgttctcccctccgagacctgaaaggagctctggaaagtctgattgaagaggaaactgggcagaagaag


 


atttaa


 


Thyroid-stimulating hormone, beta subunit (TSHB) Mouse (nucleic acid sequence):


(SEQ ID NO: 259)



Atgagtgctgccgtcctcctctccgtgctttttgctcttgcttgtgggcaagcagcatccttttgtattcccactgagtatacaatgtacgtg



 


gataggagagagtgtgcctactgcctgaccatcaacaccaccatctgtgctgggtattgtatgacacgggatatcaatggcaaact


 


gtttcttcccaaatatgcactctctcaggatgtctgtacatacagagacttcatctacagaacggtggaaataccaggatgcccgcac


 


catgttactccttatttctccttccctgtcgccataagctgcaagtgtggcaagtgtaatactgacaacagtgactgcatacacgaggct


 


gtcagaaccaactactgcaccaagccgcagtctttctatctgTactgcgccactgttcattgccaggactgtccttacgaacctgatc


 


caccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatact


 


atcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgca


 


ggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcg


 


actggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctgga


 


gacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatc


 


ggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcgg


 


aggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagat


 


acagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctg


 


atgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctg


 


cgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaacta


 


gaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattctt


 


atggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacga


 


atcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacag


 


ctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttca


 


agcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgat


 


tcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactct


 


gcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacg


 


agtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccct


 


agaagtaccagatgaatgcaaatatctggggggattttctgtttaa


 


Thyroid-stimulating hormone, beta subunit (TSHB) Human (nucleic acid sequence):


(SEQ ID NO: 260)



Atgactgctctctttctgatgtccatgctttttggccttacatgtgggcaagcgatgtctttttgtattccaactgagtatacaatgcacatcga



 


aaggagagagtgtgcttattgcctaaccatcaacaccaccatctgtgctggatattgtatgacacgggatatcaatggcaaactgtttcttc


 


ccaaatatgctctgtcccaggatgtttgcacatatagagacttcatctacaggactgtagaaataccaggatgcccactccatgttgctccc


 


tatttttcctatcctgttgctttaagctgtaagtgtggcaagtgcaatactgactatagtgactgcatacatgaagccatcaagacaaactact


 


gtaccaaacctcagaagtcttatctgTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttcca


 


acttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtga


 


aaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattct


 


atggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatca


 


ccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctc


 


aagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgct


 


gttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaat


 


cgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatc


 


cagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaa


 


aggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatg


 


ggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtc


 


tggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttct


 


ggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgac


 


tgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatc


 


tactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtac


 


atattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggag


 


cctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccgg


 


tcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggac


 


aacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaat


 


gcaaatatctggtaggattttctgtctaa


 


Cortisol-releasing factor (CRF) Mouse (nucleic acid sequence):


(SEQ ID NO: 261)



Atgcggctgcggctgctggtgtccgcgggcatgctgctggtggctctgtcgtcctgcctgccttgcagggccctgctcagcaggggat



 


ccgtcccccgagcgccgcgggccccgcagcccttgaatttcttgcagccggagcagccccagcaacctcagccggttctgatccgca


 


tgggtgaagaatacttcctccgcctggggaatctcaacagaagtcccgctgctcggctgtcccccaactccacgcccctcaccgcgggt


 


cgcggcagccgcccctcgcacgaccaggctgcggctaactttttccgcgtgttgctgcagcagctgcagatgcctcagcgctcgctcg


 


acagccgcgcggagccggccgaacgcggcgccgaggatgccctcggtggccaccagggggcgctggagagggagaggcggT


 


actgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggag


 


aatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttg


 


ccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccagga


 


acctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaa


 


gggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgta


 


aacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatc


 


accgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaag


 


gaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaa


 


gatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaagga


 


cagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcaca


 


cgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttg


 


acaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggttt


 


cacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatgga


 


aaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactg


 


actacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaa


 


gacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccac


 


cgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgt


 


aacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatg


 


ggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaggcggtcggaggagccg


 


cccatctctctggatctcaccttccaccttctgcgggaagtcttggaaatggcccgggcagagcagttagctcagcaagctcacagcaa


 


caggaaactgatggagattatcgggaaatga


 


Cortisol-releasing factor (CRF) Human (nucleic acid sequence):


(SEQ ID NO: 262)



Atgcggctgccgctgcttgtgtccgcgggagtcctgctggtggctctcctgccctgcccgccatgcagggcgctcctgagccgcggg



 


ccggtcccgggagctcggcaggcgccgcagcaccctcagcccttggatttcttccagccgccgccgcagtccgagcagccccagca


 


gccgcaggctcggccggtcctgctccgcatgggagaggagtacttcctccgcctggggaacctcaacaagagcccggccgctcccc


 


tttcgcccgcctcctcgctcctcgccggaggcagcggcagccgcccttcgccggaacaggcgaccgccaactttttccgcgtgttgctg


 


cagcagctgctgctgcctcggcgctcgctcgacagccccgcggctctcgcggagcgcggcgctaggaatgccctcggcggccacc


 


aggaggcaccggagagagaaaggcggTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagt


 


tccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgt


 


gtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaac


 


attctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtcc


 


atcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatc


 


gctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccat


 


cgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaa


 


gaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttca


 


gatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatact


 


gcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgct


 


gtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcg


 


ttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgac


 


tgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaa


 


tcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacactt


 


ccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgt


 


cgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctga


 


aggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttc


 


gccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgag


 


gggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccag


 


atgaatgcaaaaggcggtccgaggagcctcccatctccctggatctcaccttccacctcctccgggaagtcttggaaatggccagggc


 


cgagcagttagcacagcaagctccagcaacaggaaactcatggagattattgggaataa


 


Atrial natriuretic peptide (ANP) Mouse (nucleic acid sequence)


(SEQ ID NO: 263)



Atgggctccttctccatcaccctgggcttcttcctcgtcttggccttttggcttccaggccatattggagcaaatcctgtgtacagtgcggtg



 


tccaacacagatctgatggatttcaagaacctgctagaccacctggaggagaagatgccggtagaagatgaggtcatgcccccgcag


 


gccctgagtgagcagactgaggaagcaggggccgcacttagctccctccccgaggtgcctccctggactggggaggtcaacccacc


 


tctgagagacggcagtgctctagggcgcagcccctgggacccctccgatagatctgccctcttgaaaagcaaactgagggctctgctc


 


gctggccctcggagcTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtg


 


aagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaac


 


caggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaag


 


agattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggaga


 


acctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactg


 


agaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgag


 


atgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacqtcctcggaggaagatctgtaagaatcgcccca


 


gacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaac


 


aactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtctt


 


ctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtgga


 


gacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggaca


 


tactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaac


 


acttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagt


 


agaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactg


 


gcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatatt


 


agagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctg


 


tgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaa


 


agtgatcttgatcagaaatgtaacgtgtgccaacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaaca


 


gggtttctgtaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaa


 


acggagcctacgaagatccagctgcttcgggggtaggattgacaggattggagcccagagtggactaggctgcaacagcttccggta


 


ccgaagataa


 


Atrial natriuretic peptide (ANP) Human (nucleic acid sequence):


(SEQ ID NO: 264)



atgagctccttctccaccaccaccgtgagcttcctccttttactggcattccagctcctaggtcagaccagagctaatcccatgtacaatgc



 


cgtgtccaacgcagacctgatggatttcaagaatttgctggaccatttggaagaaaagatgcctttagaagatgaggtcgtgcccccaca


 


agtgctcagtgagccgaatgaagaagcgggggctgctctcagccccctccctgaggtgcctccctggaccggggaagtcagcccag


 


cccagagagatggaggtgccctcgggcggggcccctgggactcctctgatcgatctgccctcctaaaaagcaagctgagggcgctgc


 


tcactgcccctcggagcTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgt


 


gaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaa


 


ccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaa


 


gagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggag


 


aacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctact


 


gagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttga


 


gatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgcccc


 


agacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaa


 


caactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtc


 


ttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtgg


 


agacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggac


 


atactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaa


 


cacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtag


 


tagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactg


 


gcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatatt


 


agagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctg


 


tgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaa


 


agtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaaca


 


gggtttctgtgaccacgcatgggagttcaagaaagatgctacataaagcatggagacaccctagaagtaccagatgaatgcaa


 


acggagcctgcggagatccagctgcttcgggggcaggatggacaggattggagcccagagcggactgggctgtaacagcttccggt


 


actga


 


Brain natriuretic peptide (BNP) Mouse (nucleic acid sequence):


(SEQ ID NO: 265)



atggatctcctgaaggtgctgtcccagatgattctgtttctgcttttcctttatctgtcaccgctgggaggtcactcctatcctctgggaagtcc



 


tagccagtctccagagcaattcaagatgcagaagctgctggagctgataagagaaaagtcggaggaaatggcccagagacagctctt


 


gaaggaccaaggcctcacaaaagaacacccaaaaagagtccttcggtctTactgcgccactgttcattgccaggactgtccttacga


 


acctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgaga


 


gacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtaga


 


ggccgcaggatggtttagaacattctatggaaagagattccagttccaggaactggtacatacgtgttgggtcaaggaaccaagg


 


gcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtg


 


gctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgta


 


caccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgac


 


atcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatga


 


tggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatgg


 


aaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacac


 


catctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcc


 


cgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaa


 


ggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagag


 


aaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgt


 


cccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaa


 


gttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatt


 


tcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagct


 


gaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacga


 


tgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatg


 


gagacaccctagaagtaccagatgaatgcaaacggtctcaaggcagcaccctccgggtccagcagagacctcaaaattccaaggt


 


gacacatatctcaagctgctttgggcacaagatagaccggatcggatccgtcagtcgtttgggctgtaacgcactgaagttgttgtag


 


Brain natriuretic peptide (BNP) Human (nucleic acid sequence):


(SEQ ID NO: 266)



Atggatccccagacagcaccttcccgggcgctcctgctcctgctcttcttgcatctggctttcctgggaggtcgttcccacccgctgggc



 


agccccggttcagcctcggacttggaaacgtccgggttacaggagcagcgcaaccaatttgcagggcaaactgtcggagctgcaggtg


 


gagcagacatccctggagcccctccaggagagcccccgtcccacaggtgtctggaagtcccgggaggtagccaccgagggcatcc


 


gtgggcaccgcaaaatggtcctctacaccctgcgggcaccacgaagcTactgcgccactgttcattgccaggactgtccttacgaac


 


ctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagaga


 


catactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagagg


 


ccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggc


 


ggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggc


 


tggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtaca


 


ccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatc


 


ctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatgg


 


aagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaa


 


atcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacacca


 


tctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccg


 


aaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaagg


 


agattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaa


 


agtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcc


 


cgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagtt


 


caacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttca


 


gtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctga


 


acgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatg


 


catgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatgga


 


gacaccctagaagtaccagatgaatgcaaacgaagccgaagccccaagatggtgcaagggtctggctgctttgggaggaagatgg


 


accggatcagctcctccagtggcctgggctgcaaagtgctgaggcggcattaa


 


Renin Mouse (nucleic acid sequence):


(SEQ ID NO: 267)



Atggacagaaggaggatgcctctctgggcactcttgttgctctggagtccttgcaccttcagtctcccaacacgcaccgctacctttgaa



 


cgaatcccgctcaagaaaatgccttctgtccgggaaatcctggaggagcggggagtggacatgaccaggctcagtgctgaatggggc


 


gtattcacaaagaggTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtg


 


aagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaac


 


caggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaag


 


agattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggaga


 


acctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactg


 


agaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgag


 


atgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgcccca


 


gacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaac


 


aactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtctt


 


ctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtgga


 


gacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggaca


 


tactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaac


 


acttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagt


 


agaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactg


 


gcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatatt


 


agagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctg


 


tgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaa


 


agtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaaca


 


gggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaa


 


aaagaggccttccttgaccaatcttacctcccccgtggtcctcaccaactacctgaatacccagtactacggcgagattggcatcggtac


 


cccaccccagaccttcaaagtcatctttgacacgggttcagccaacctctgggtgccctccaccaagtgcagccgcctctaccttgcttgt


 


gggattcacagcctctatgagtcctctgactcctccagctacatggagaacgggtccgacttcaccatccactacggatcagggagagt


 


caaaggtttcctcagccaggactcggtgactgtgggtggaatcactgtgacacagacctttggagaggtcaccgagctgcccctgatcc


 


ctttcatgctggccaagtttgacggtgttctaggcatgggctttcccgctcaggccgttggcggggttacccctgtctttgaccacattctct


 


cccagggggtgctaaaggaggaagtgttctctgtctactacaacaggggttcccacctgctggggggcgaggtggtgctaggaggta


 


gcgacccgcagcattatcaaggcaattttcactatgtgagcatcagcaagactgactcctggcagatcacgatgaagggggtgtctgtg


 


gggtcttccaccctgctatgtgaagaaggctgtgcggtagtggtggacactggttcatcctttatctcggctcctacgagctccctgaagtt


 


gatcatgcaagccctgggagccaaggagaagagaatagaagaatatgttgtgaactgtagccaggtgcccaccctccccgacatttcc


 


tttgacctgggaggcagggcctacacactcagcagtacggactacgtgctacagtatcccaacaggagagacaagctgtgcacactg


 


gctctccatgccatggacatcccaccacccactgggcctgtctgggtcctgggtgccaccttcatccgcaagttctatacagagtttgatc


 


ggcataacaatcgcattggattcgccttggcccgctaa


 


Renin Human (nucleic acid sequence):


(SEQ ID NO: 268)



Atggatggatggagaaggatgcctcgctggggactgctgctgctgctctggggctcctgtacctttggtctcccgacagacaccacca



 


cctttaaacggTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagct


 


aaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccagga


 


aaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattc


 


cagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctg


 


gatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaat


 


cccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgcca


 


ggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacaca


 


gcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgc


 


tattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagc


 


cgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagc


 


gagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttcta


 


cgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttggga


 


tgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactca


 


ttgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatg


 


gtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatcc


 


attcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgac


 


ccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatctt


 


gatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctg


 


tgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaaacgga


 


tcttcctcaagagaatgccctcaatccgagaaagcctgaaggaacgaggtgtggacatggccaggcttggtcccgagtggagccaac


 


ccatgaagaggctgacacttggcaacaccacctcctccgtgatcctcaccaactacatggacacccagtactatggcgagattggcatc


 


ggcaccccaccccagaccttcaaagtcgtctttgacactggttcgtccaatgtttgggtgccctcctccaagtgcagccgtctctacactg


 


cctgtgtgtatcacaagctcttcgatgcttcggattcctccagctacaagcacaatggaacagaactcaccctccgctattcaacagggac


 


agtcagtggctttctcagccaggacatcatcaccgtgggtggaatcacggtgacacagatgtttggagaggtcacggagatgcccgcct


 


tacccttcatgctggccgagtttgatggggttgtgggcatgggcttcattgaacaggccattggcagggtcacccctatcttcgacaacat


 


catctcccaaggggtgctaaaagaggacgtcttctctttctactacaacagagattccgagaattcccaatcgctgggaggacagattgt


 


gctgggaggcagcgacccccagcattacgaagggaatttccactatatcaacctcatcaagactggtgtctggcagattcaaatgaagg


 


gggtgtctgtggggtcatccttccagctctgtgaagacggctgcctggcattggtagacaccggtgcatcctacatctcaggttctaccag


 


ctccatagagaagctcatggaggccttgggagccaagaagaggctgtttgattatgtcgtgaagtgtaacgagggccctacactccccg


 


acatctctttccacctgggaggcaaagaatacacgctcaccagcgcggactatgtatttcaggaatcctacagtagtaaaaagctgtgca


 


cactggccatccacgccatggatatcccgccacccactggacccacctgggccctgggggccaccttcatccgaaagttctacacaga


 


gtttgatcggcgtaacaaccgcattggcttcgccttggcccgctga


 


Galanin Mouse (nucleic acid sequence):


(SEQ ID NO: 269)



Atggccagaggcagcgttatcctgctaggctggctcctgttggttgtgaccctgtcagccactctgggacttgggatgcctgcaaagga



 


gaagagaggtTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagct


 


aaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccagga


 


aaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattc


 


cagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctg


 


gatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaat


 


cccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgcca


 


ggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacaca


 


gcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgc


 


tattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagc


 


cgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagc


 


gagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttcta


 


cgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttggga


 


tgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactca


 


ttgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatg


 


gtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatcc


 


attcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgac


 


ccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatctt


 


gatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctg


 


tgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaagagag


 


gttggaccctgaacagcgctggctaccttctgggcccacatgccattgacaaccacagatcatttagcgacaagcatggcctcacaggc


 


aagagggagttacaactggaggtggaggaaaggagaccaggaagtgttgatgtgcccctgcctgagagcaacattgtccgcactata


 


atggagtttctcagtttcttgcaccttaaagaggccggggccctcgacagcctgcctggcatccccttggccacctcctcagaagaccta


 


gagaagtcctga


 


Galanin Human (nucleic acid sequence):


(SEQ ID NO: 270)



Atggcccgaggcagcgccctcctgctcgcctccctcctcctcgccgcggccctttctgcctctgcggggctctggtcgccggccaag



 


gaaaaacgaggcTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaa


 


gctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaacca


 


ggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagag


 


attccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaac


 


ctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgag


 


aatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatg


 


ccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagac


 


acagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaact


 


cgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctgg


 


agccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacg


 


agcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactt


 


tctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttg


 


ggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaa


 


ctcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaag


 


atggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagaga


 


tccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatct


 


gacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtga


 


tcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggttt


 


ctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaaac


 


gaggctggaccctgaacagcgcgggctacctgctgggcccacatgccgttggcaaccacaggtcattcagcgacaagaatggcctca


 


ccagcaagcgggagctgcggcccgaagatgacatgaaaccaggaagctttgacaggtccatacctgaaaacaatatcatgcgcacaa


 


tcattgagtttctgtctttcttgcatctcaaagaggccggtgccctcgaccgcctcctggatctccccgccgcagcctcctcagaagacatc


 


gagcggtcctga


 


Orexin Mouse (nucleic acid sequence):


(SEQ ID NO: 271)



Atgaactttccttctacaaaggttccctgggccgccgtgacgctgctgctgctgctactgctgccgccggcgctgctgtcgcttggggt



 


ggacgcacagcctctgcccgactgctgtcgccagaagacgtgttcctgccgtctctacgaactgttgcacggagctggcaaccacg


 


ctgcgggtatcctgactctgggaaagcggcggcctggacctccaggcctccagggacggctgcagcgcctccttcaggccaacgg


 


taaccacgcagctggcatcctgaccatgggccgccgcTactgcgccactgttcattgccaggactgtccttacgaacctgatccacc


 


aaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatca


 


gatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggat


 


ggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactgg


 


aaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacat


 


cattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcg


 


aggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggagga


 


agatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacag


 


acttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatga


 


cgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgc


 


cttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactaga


 


ggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttat


 


ggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaat


 


caggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagct


 


ctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaa


 


gcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgatt


 


cttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctg


 


caatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacga


 


gtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacacccta


 


gaagtaccagatgaatgcaaaggccgccgcgcaggcgcagagctagagccacatccctgctctggtcgcggctgtccgaccgtaa


 


ctaccaccgctttagcaccccggggagggtccggagtctga


 


Orexin Human (nucleic acid sequence):


(SEQ ID NO: 272)



Atgaaccttccttccacaaaggtctcctgggccgccgtgacgctactgctgctgctgctgctgctgccgcccgcgctgttgtcgtccgg



 


ggcggctgcacagcccctgcccgactgctgtcgtcaaaagacttgctcttgccgcctctacgagctgctgcacggcgcgggcaatcac


 


gcggccggcatcctcacgctgggcaagcggaggtccgggcccccgggcctccagggtcggctgcagcgcctcctgcaggccagc


 


ggcaaccacgccgcgggcatcctgaccatgggccgccgcTactgcgccactgttcattgccaggactgtccttacgaacctgatcc


 


accaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatacta


 


tcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcag


 


gatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgac


 


tggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggaga


 


catcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcgg


 


cgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggag


 


gaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatac


 


agacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgat


 


gacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgc


 


gccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaacta


 


gaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattctt


 


atggccgcgcgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacga


 


atcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacag


 


ctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttca


 


agcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgat


 


tcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactct


 


gcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacg


 


agtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccct


 


agaagtaccagatgaatgcaaaggccgccgcgcaggcgcagagccagcgccgcgcccctgcctcgggcgccgctgttccgccc


 


cggccgccgcctccgtcgcgcccggaggacagtccgggatctga


 


Ghrelin-Obestatin Mouse (nucleic acid sequence):


(SEQ ID NO: 273)



Atgctgtcttcaggcaccatctgcagtttgctgctactcagcatgctctggatggacatggccatggcaggctccagcttcctgagccca



 


gagcaccagaaagcccagcagagaaaggaatccaagaagccaccagctaaactgcagccacgagctTactgcgccactgttcattg


 


ccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagct


 


gtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgt


 


aattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgtt


 


gggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgacc


 


aagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgacc


 


ctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttc


 


aaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctct


 


gtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttg


 


acggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaacccca


 


tcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacag


 


ggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagatacca


 


attccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgact


 


cttacactgaagtagagaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttgga


 


ggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacct


 


gaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcgg


 


taactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaaga


 


acagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagc


 


ctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaaga


 


atgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaccacgagctctggaaggctggctccacccagagga


 


cagaggacaagcagaagagacagaggaggagctggagatcaggttcaatgctcccttcgatgttggcatcaagctgtcaggagctca


 


gtatcagcagcatggccgggccctggggaagtttcttcaggatatcctctgggaagaggtcaaagaggcgccagctgacaagtaa


 


Ghrelin-Obestatin Human (nucleic acid sequence):


(SEQ ID NO: 274)



Atgccctccccagggaccgtctgcagcctcctgctcctcggcatgctctggctggacttggccatggcaggctccagcttcctgagcc



 


ctgaacaccagagagtccagagaaaggagtcgaagaagccaccagccaagctgcagccccgagctTactgcgccactgttcattgc


 


caggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgt


 


ggcacctgacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaa


 


ttgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgg


 


gtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaa


 


gacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccct


 


atcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttca


 


aactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctg


 


tggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttga


 


cggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccat


 


caacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagg


 


gagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaa


 


ttccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactc


 


ttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggag


 


gagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctg


 


aagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggt


 


aactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaaga


 


acagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagc


 


ctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaaga


 


atgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaccccgagctctagcaggctggctccgcccggaagat


 


ggaggtcaagcagaaggggcagaggatgaactggaagtccggttcaacgccccctttgatgttggaatcaagctgtcaggggttcagt


 


accagcagcacagccaggccctggggaagtttcttcaggacatcctctgggaagaggccaaagaggccccagccgacaagtga


 


Cholecystokinin Mouse (nucleic acid sequence):


(SEQ ID NO: 275)



Atgaagagcggcgtatgtctgtgcgtggtgatggcagtcctagctgctggccgccctggcgcagccggtagtccctgcagaagctacg



 


gaccccgtggagcagcgggcgcaagaggcgccccgaaggcagctgcgggctTactgcgccactgttcattgccaggactgtcctt


 


acgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcac


 


gagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcaga


 


gtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaac


 


caagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactg


 


gaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaa


 


cccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtga


 


tcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaa


 


aatgatggaagatacagacttcacttcagatccaagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccact


 


ctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctacta


 


ctacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcg


 


ctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtcc


 


ctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaa


 


gtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccg


 


tgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggt


 


ggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaacc


 


aggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacct


 


gaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtc


 


gaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataa


 


agcatggagacaccctagaagtaccagatgaatgcaaacgaaggcagctgcgggctgtgctccggacggacggcgagccccga


 


gcgcgcctgggcgcactgctagcgcgatacatccagcaggtccgcaaagctccttctggccgcatgtccgttcttaagaacctgcaga


 


gcctggaccccagccatagaataagtgaccgggactacatgggctggatggattttggccggcgcagtgccgaggactacgaatacc


 


catcgtag


 


Cholecystokinin Human (nucleic acid sequence):


(SEQ ID NO: 276)



atgaacagcggcgtgtgcctgtgcgtgctgatggcggtactggcggctggcgccctgacgcagccggtgcctcccgcagatcccgc



 


gggctccgggctgcagcgggcagaggaggcgccccgtaggcagctgagggtaTactgcgccactgttcattgccaggactgtcctt


 


acgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcac


 


gagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcaga


 


gtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaac


 


caagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactg


 


gaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaa


 


cccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtga


 


tcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaa


 


aatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccact


 


ctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctacta


 


ctacaccatctcctgcgcccttcgcccgctgtatgggtggagaacgagcgagcctcacacgtgctgcttgactacagggagacgtgcg


 


ctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtcc


 


ctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaa


 


gtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccg


 


tgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggt


 


ggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaacc


 


aggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacct


 


gaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtc


 


gaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataa


 


agcatggagacaccctagaagtaccagtgaatgcaaacgtaggcagctgagggtatcgcagagaacggatggcgagtcccgag


 


cgcacctgggcgccctgctggcaagatacatccagcaggcccggaaagctccttctggacgaatgtccatcgttaagaacctgcagaa


 


cctggaccccagccacaggataagtgaccgggactacatgggctggatggattttggccgtcgcagtgccgaggagtatgagtaccc


 


ctcctag


 


Gastrin Mouse (nucleic acid sequence):


(SEQ ID NO: 277)



Atgcctcgactgtgtgtgtacatgctggtcttagtgctggctctagctaccttctcggaagcttcttggaagccccgctcccagctacagg



 


atgcatcatctggaccagggaccaatgaggacctggaacagcgccagttcaacaagctgggctcagcctctcaccatcgaaggcagc


 


tggggccccagggtcctcaacacttcatagcagacctgtccaagaagcagaggccacgaatggaggaagaagaagaggcctacgg


 


atggatggactttggccgccgcagtTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttcca


 


acttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtga


 


aaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattct


 


atggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatca


 


ccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctc


 


aagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgct


 


gttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaat


 


cgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatc


 


cagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaa


 


aggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatg


 


ggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtc


 


tggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttct


 


ggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgac


 


tgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatc


 


tactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtac


 


atattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggag


 


cctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccgg


 


tcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggac


 


aacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaat


 


gcaaacgccgcagtgctgaggaagaccagtag


 


Gastrin Human (nucleic acid sequence):


(SEQ ID NO: 278)



Atgcagcgactatgtgtgtatgtgctgatctttgcactggctctggccgccttctctgaagcttcttggaagccccgctcccagcagccag



 


atgcacccttaggtacaggggccaacagggacctggagctaccctggctggagcagcagggcccagcctctcatcatcgaaggcag


 


ctgggaccccagggtcccccacacctcgtggcagacccgtccaagaagcagggaccatggctggaggaagaagaagaagcctatg


 


gatggatggacttcggccgccgcagtTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttc


 


caacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgt


 


gaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacat


 


tctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccat


 


caccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcg


 


ctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatc


 


gctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaag


 


aatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcag


 


atccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactg


 


caaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgt


 


atgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttt


 


tgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactg


 


tttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatc


 


gactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttc


 


catctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtc


 


gtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaa


 


ggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcg


 


ccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgagg


 


ggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagat


 


gaatgcaaacgccgcagtgctgaggatgagaactaa


 


Protachykinin-1 (Substance P, Neurokinin A, Neuropeptide K, Neuropeptide gamma) Mouse


(nucleic acid sequence):


(SEQ ID NO: 279)



atgaaaatcctcgtggccgtggcggtcttttttctcgtttccactcaactgtttgcagaggaaatcgatgccaacgatgatctaaattattggt



 


ccgactggtccgacagtgaccagatcaaggaggcaatgccggagccctttgagcatcttctgcagagaatcgcccgaagaTactgcg


 


ccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgta


 


ttgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaat


 


gtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctgg


 


tacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaaggggg


 


ctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggt


 


ggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtc


 


attgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatga


 


tctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaa


 


ccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctg


 


ccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgct


 


gcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaa


 


gcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacac


 


aggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaaca


 


gattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactac


 


agccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagactt


 


gcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccggg


 


atgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacg


 


tgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggag


 


ttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaacgaagacccaagcctcagcagttct


 


ttggattaatgggcaagcgggatgctgattcctcagttgaaaaacaagtggccctgttaaaggctctttatggacatggccagatctctca


 


caaaaggcataaaacagattcctttgttggactaatgggcaaaagagctttaaattctgtggcttatgaaagaagcgcgatgcagaactac


 


gaaagaagacgtaaataa


 


Protachykinin-1 (Substance P, Neurokinin A, Neuropeptide K, Neuropeptide gamma) Human


(nucleic acid sequence):


(SEQ ID NO: 280)



atgaaaatcctcgtggccttggcagtcttttttcttgtctccactcagctgtttgcagaagaaataggagccaatgatgatctgaattactggt



 


ccgactggtacgacagcgaccagatcaaggaggaactgccggagccctttgagcatcactgcagagaatcgcccggagaTactgc


 


gccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgt


 


attgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaa


 


tgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctg


 


gtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggg


 


gctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacg


 


gtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccg


 


tcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatg


 


atctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatca


 


accaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagct


 


gccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgc


 


tgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaa


 


agcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcaca


 


caggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaac


 


agattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgacta


 


cagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagact


 


tgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgg


 


gatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaac


 


gtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatggga


 


gttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaacggagacccaagcctcagcagtt


 


ctttggattaatgggcaaacgggatgctgattcctcaattgaaaaacaagtggccctgttaaaggctctttatggacatggccagatctctc


 


acaaaagacataaaacagattcctttgttggactaatgggcaaaagagctttaaattctgtggcttatgaaaggagtgcaatgcagaattat


 


gaaagaagacgttaa


 


Proenkephalin-A Mouse (nucleic acid sequence):


(SEQ ID NO: 281)



atggcgcggttcctgaggctttgcacctggctgctggcgcttgggtcctgcctcctggctacagtgcaggcggaatgcagccaggact



 


gcgctaaatgcagctaccgcctggttcgcccaggcgacatcaatttcctggcgtgcacactggaatgtgaaggacagctgccttctttca


 


aaatctgggagacctgcaaggatctcctgcaggtgtccaggcccgagttcccttgggataacatcgacatgtacaaagacagcagcaa


 


acaggatgagagccacttgctagccaagaagtacggaggcttcatgaaacggtacggaggcttcatgaagaagatggacgagctatat


 


cccatggagccagaagaagaagcgaacggaggagagatccttgccaagaggtatggcggcttcatgaagaaggatgcagatgagg


 


gagacaccttggccaactcctccgatctgctgaaagagctactgggaacgggagacaaccgtgcgaaagacagccaccaacaagag


 


agcaccaacaatgacgaagacatgagcaagaggtatgggggcttcatgagaagcctcaaaagaagcccccaactggaagatgaagc


 


aaaagagctgcagaagcgctacgggggcttcatgagaaggTactgcgccactgttcattgccaggactgtccttacgaacctgatcc


 


accaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatacta


 


tcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcag


 


gatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgac


 


tggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggaga


 


catcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcgg


 


cgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggag


 


gaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatac


 


agacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgat


 


gacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgc


 


gccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaacta


 


gaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattctt


 


atggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacga


 


atcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacag


 


ctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttca


 


agcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgat


 


tcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactct


 


gcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacg


 


agtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccct


 


agaagtaccagatgaatgcaaaagaagggtgggacgccccgagtggtggatggactaccagaagaggtatgggggcttcctgaa


 


gcgctttgctgagtctctgccctccgatgaagaaggcgaaaattactcgaaagaagttcctgagatagagaaaagatacgggggctttat


 


gcggttctga


 


Proenkephalin-A Human (nucleic acid sequence):


(SEQ ID NO: 282)



atggcgcggttcctgacactttgcacttggctgctgttgctcggccccgggctcctggcgaccgtgcgggccgaatgcagccaggattg



 


cgcgacgtgcagctaccgcctagtgcgcccggccgacatcaacttcctggcttgcgtaatggaatgtgaaggtaaactgccttctctga


 


aaatttgggaaacctgcaaggagctcctgcagctgtccaaaccagagcttcctcaagatggcaccagcaccctcagagaaaatagcaa


 


accggaagaaagccatttgctagccaaaaggtatgggggcttcatgaaaaggtatggaggcttcatgaagaaaatggatgagctttatc


 


ccatggagccagaagaagaggccaatggaagtgagatcctcgccaagcggtatgggggcttcatgaagaaggatgcagaggagga


 


cgactcgctggccaattcctcagacctgctaaaagagcttctggaaacaggggacaaccgagagcgtagccaccaccaggatggca


 


gtgataatgaggaagaagtgagcaagagatatgggggcttcatgagaggcttaaagagaagcccccaactggaagatgaagccaaa


 


gagctgcagaagcgatatgggggcttcatgagaagaTactgcgccactgttcattgccaggactgtccttacgaacctgatccacca


 


aacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcag


 


atggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatg


 


gtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactgga


 


aggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatc


 


attgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcga


 


ggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaa


 


gatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacaga


 


cttacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgac


 


gttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgcct


 


tcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagagg


 


aacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatgg


 


ccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatca


 


ggaacaatcgactgtagtagaactacattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctc


 


agaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagc


 


aactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattctt


 


tgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaa


 


tagtctcttcgccgttcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgtgcatgtacgagtat


 


tgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaa


 


gtaccagatgaatgcaaaagaagagtaggtcgcccagagtggtggatggactaccagaaacggtatggaggtttcctgaagcgcttt


 


gccgaggctctgccctccgacgaagaaggcgaaagttactccaaagaagttcctgaaatggaaaaaagatacggaggatttatgagat


 


tttaa


 


Proenkephalin-B Mouse (nucleic acid sequence):


(SEQ ID NO: 283)



atggcgtggtccaggctgatgctggcagcttgcctcctcgtgatgccctctaatgttatggcggactgcctgtccctgtgctccctgtgt



 


gcagtgaggattcaggatgggccccgtcccatcaaccccctgatttgctccctggagtgccaggacctggtgccgccctcagagga


 


gtgggagacatgccggggcttctcatcttttctcaccctgacggtctctgggctccgtggcaaggatgacttggaagatgaggttgctt


 


tggaagaaggctacagtgcactagccaagctcttggaacccgtcctgaaggagctggagaaaagccgactccttaccagcgtcc


 


cagaggaaaagttcaggggtctctccagcagctttggcaacggaaaagaatctgagctggcgggtgctgaccggatgaatgatg


 


aagccgcacaggcgggcacgctccattttaatgaggaggacttgagaaaacaggccaaacgctatggcggctttttgcgcaaata


 


ccccaagaggTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagc


 


taaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccagg


 


aaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagatt


 


ccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacct


 


ggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgaga


 


atcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgc


 


caggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagaca


 


cagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactc


 


gctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctgga


 


gccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacga


 


gcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttc


 


tacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgg


 


gatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaact


 


cattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaaga


 


tggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatc


 


cattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctga


 


cccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatct


 


tgatcagaaatgtaaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttct


 


gtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaagagg


 


agttccgagatggcccgggatgaggacgggggccaggatggggatcaggtagggcatgaggacctgtacaaacgctatgggggctt


 


cctgcggcgcattcgccccaagctgaagtgggacaaccagaagcgctatggtggtttcctgcggcgtcagttcaaggtggtgacgcg


 


gtcccaggagaaccccaatacctattctgaagatttagatgtttga


 


Proenkephalin-B Human (nucleic acid sequence):


(SEQ ID NO: 284)



atggcctggcaggggctggtcctggctgcctgcctcctcatgttcccctccaccacagcggactgcctgtcgcggtgctccttgtgtgct



 


gtaaagacccaggatggtcccaaacctatcaatcccctgatttgctccctgcaatgccaggctgccctgctgccctctgaggaatggga


 


gagatgccagagctttctgtcttttttcaccccctccacccttgggctcaatgacaaggaggacttggggagcaagtcggttggggaagg


 


gccctacagtgagctggccaagctctctgggtcattcctgaaggagctggagaaaagcaagtttctcccaagtatctcaacaaaggaga


 


acactctgagcaagagcctggaggagaagctcaggggtctctctgacgggtttagggagggagcagagtctgagctgatgagggatg


 


cccagctgaacgatggtgccatggagactggcacactctatctcgctgaggaggaccccaaggagcaggtcaaacgcTactgcgcc


 


actgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattg


 


atagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtg


 


tcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtac


 


atacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgt


 


gctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtgga


 


gctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattg


 


agttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctct


 


ggcctctgtggagatcttaaaatgatggaagtacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccag


 


gagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgc


 


aaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttg


 


actacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaa


 


gataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacagga


 


atgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattc


 


tggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagcca


 


tcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggt


 


atttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgca


 


ccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgc


 


cacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaa


 


gaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaaacgctatgggggctttttgcgcaaatac


 


cccaagaggagctcagaggtggctggggagggggacggggatagcatgggccatgaggacctgtacaaacgctatgggggcttctt


 


gcggcgcattcgtcccaagctcaagtgggacaaccagaagcgctatggcggttttctccggcgccagttcaaggtggtgactcggtctc


 


aggaagatccgaatgcttactctggagagctttttgatgcataa


 


Insulin-like growth hormone 1 (IGF-1) Mouse (nucleic acid sequence):


(SEQ ID NO: 285)



atggggaaaatcagcagccttccaactcaattatttaagatctgcctctgtgacttcttgaagataaagatacacatcatgtcgtcttcacac



 


ctcttctacctggcgctctgcttgctcaccttcaccagctccaccacagctggaccagagaccctttgcggggctgagctggtggatgct


 


cttcagttcgtgtgtggaccgaggggcttttacttcaacaagcccacaggctatggctccagcattcggaggTactgcgccactgttcat


 


tgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcag


 


ctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatg


 


taattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgt


 


tgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgacc


 


aagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgacc


 


ctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttc


 


aaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctct


 


gtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttg


 


acggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaacccca


 


tcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacag


 


ggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagatacca


 


attccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgact


 


cttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttgga


 


ggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacct


 


gaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcgg


 


taactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaaga


 


acagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagc


 


ctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaaga


 


atgctacataaagcatggagacaccctagaagtaccagatgaatgcaaatccagcattcggagggcacctcagacaggcattgtg


 


gatgagtgttgcttccggagctgtgatctgaggagactggagatgtactgtgccccactgaagcctacaaaagcagcccgctctatccgt


 


gcccagcgccacactgacatgcccaagactcagaagtccccgtccctatcgacaaacaagaaaacgaagctgcaaaggagaaggaa


 


aggaagtacatttgaagaacacaagtag


 


Insulin-like growth hormone 1 (IGF-1) Human (nucleic acid sequence):


(SEQ ID NO: 286)



atgggaaaaatcagcagtcttccaacccaattatttaagtgctgcttttgtgatttcttgaaggtgaagatgcacaccatgtcctcctcgcatc



 


tcttctacctggcgctgtgcctgctcaccttcaccagctctgccacggctggaccggagacgctctgcggggctgagctggtggatgct


 


cttcagttcgtgtgtggagacaggggcttttatttcaacaagcccacagggtatggctccagcagtcggaggTactgcgccactgttcat


 


tgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcag


 


ctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatg


 


taattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgt


 


tgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgacc


 


aagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgacc


 


ctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttc


 


aaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctct


 


gtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttg


 


acggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaacccca


 


tcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacag


 


ggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagatacca


 


attccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgact


 


cttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttgga


 


ggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacct


 


gaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcgg


 


taactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaaga


 


acagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagc


 


ctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaaga


 


atgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaagcagtcggagggcgcctcagacaggcatcgtgga


 


tgagtgctgcttccggagctgtgatctaaggaggctggagatgtattgcgcacccctcaagcctgccaagtcagctcgctctgtccgtgc


 


ccagcgccacaccgacatgcccaagacccagaagtatcagcccccatctaccaacaagaacacgaagtctcagagaaggaaagga


 


agtacatttgaagaacgcaagtag


 


Insulin-like growth hormone 2 (IGF-2)Mouse (nucleic acid sequence):


(SEQ ID NO: 287)



atgggcggcagcgtcgccggcttccaggtaccaatggggatcccagtggggaagtcgatgttggtgcttctcatctctttggccttcgcc



 


ttgtgctgcatcgctgcttacggccccggagagactctgtgcggaggggagcttgagacacgcttcagtagtctgacggaccgcggc


 


ttctacttcagcaggccttcaagccgtgccaaccgtcgcagccgtggcatcgtggaagagtgctgcttccgcagctgcgacctggccct


 


cctggagacatactgtgccacccccgccaagtccgagagggacTactgcgccactgttcattgccaggactgtccttacgaacctga


 


tccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacata


 


ctatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgc


 


aggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggc


 


gactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctgg


 


agacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccat


 


cggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcg


 


gaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaag


 


atacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcc


 


tgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcc


 


tgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaac


 


tagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagatt


 


cttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtac


 


gaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtac


 


agctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaact


 


tcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgat


 


gattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgac


 


tctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgta


 


cgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacac


 


cctagaagtaccagatgaatgcaaagagagggacgtgtctacctctcaggccgtacttccggacgacttccccagataccccgtggg


 


caagttcttccaatatgacacctggagacagtccgcgggacgcctgcgcagaggcctgcctgccctcctgcgtgcccgccggggtcg


 


catgcttgccaaagagctcaaagagttcagagaggccaaacgtcatcgtcccctgatcgtgttaccacccaaagaccccgcccacggg


 


ggagcctcttcggagatgtccagcaaccatcagtga


 


Insulin-like growth hormone 2 (IGF-2) Human (nucleic acid sequence):


(SEQ ID NO: 288)



atgggaatcccaatggggaagtcgatgctggtgcttctcaccttcttggccttcgcctcgtgctgcattgctgcttaccgccccagtgag



 


accctgtgcggcggggagctggtggacaccctccagttcgtctgtggggaccgcggcttctacttcagcaggcccgcaagccgtgt


 


gagccgtcgcagccgtggcatcgttgaggagtgctgtttccgcagctgtgacctggccctcctggagacgtactgtgctacccccgc


 


caagtccgagagggacTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctg


 


tgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaa


 


ccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaa


 


gagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggag


 


aacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctact


 


gagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttga


 


gatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgcccc


 


agacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaa


 


caactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtc


 


ttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtgg


 


agacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggac


 


atactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaa


 


cacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtag


 


tagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactg


 


gcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatatt


 


agagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctg


 


tgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaa


 


agtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaaca


 


gggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaa


 


agagagggacgtgtcgacccctccgaccgtgcttccggacaacttccccagataccccgtgggcaagttcttccaatatgacacctgg


 


aagcagtccacccagcgcctgcgcaggggcctgcctgccctcctgcgtgcccgccggggtcacgtgctcgccaaggagctcgagg


 


cgttcagggaggccaaacgtcaccgtcccctgattgctctacccacccaagaccccgcccacgggggcgcccccccagagatggcc


 


agcaatcggaagtga


 


Parathyroid hormone (PTH) Mouse (nucleic acid sequence):


(SEQ ID NO: 289)



atgatgtctgcaaacaccgtggctaaagtgatgatcatcatgctggcagtctgtcttcttacccaaacggatgggaaacccgtgaggaag



 


agaTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaa


 


ggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaaca


 


tgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttcc


 


aggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaa


 


ccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatca


 


ctgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttca


 


acatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaac


 


aaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcag


 


cctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtaca


 


aggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcct


 


cacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatac


 


atttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaa


 


ggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttga


 


tggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacat


 


actgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatg


 


gtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaac


 


ccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcaga


 


aatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccac


 


gcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaagagagctgtcagt


 


gaaatacagcttatgcacaacctgggcaaacacctggcctccatggagaggatgcaatggctgagaaggaagctgcaagatatgcac


 


aattttgttagtcttggagtccaaatggctgccagagatggcagtcaccagaagcccaccaagaaggaggaaaatgtccttgttgatggc


 


aatccaaaaagtcttggtgagggagacaaagctgatgtggatgtattagttaaatcaaaatctcagtaa


 


Parathyroid hormone (PTH) Human (nucleic acid sequence):


(SEQ ID NO: 290)



atgatacctgcaaaagacatggctaaagttatgattgtcatgttggcaatttgttttcttacaaaatcggatgggaaatctgttaagaagaga



 


Tactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaagga


 


gaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgtt


 


gccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccagg


 


aacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaacca


 


agggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgt


 


aaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaaca


 


tcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaa


 


ggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagccta


 


agatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaagg


 


acagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcac


 


acgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacattt


 


gacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtt


 


tcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatgg


 


aaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatact


 


gactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggta


 


agacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaaccca


 


ccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaat


 


gtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgca


 


tgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaagagatctgtgagtgaa


 


atacagcttatgcataacctgggaaaacatctgaactcgatggagagagtagaatggctgcgtaagaagctgcaggatgtgcacaatttt


 


gttgcccttggagctcctctagctcccagagatgctggttcccagaggccccgaaaaaaggaagacaatgtcttggttgagagccatga


 


aaaaagtcttggagaggcagacaaagctgatgtgaatgtattaactaaagctaaatcccagtga


 


Parathyroid hormone-related protein (PTHrP) Mouse (nucleic acid sequence):


(SEQ ID NO: 291)



atgctgcggaggctggttcagcagtggagtgtcctggtattcctgctcagctactccgtgccctcccgcgggcgttcggtggaggggct



 


tggccgcaggctcaaacgcTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttc


 


ctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaat


 


aaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatgg


 


aaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctg


 


gagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagct


 


actgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgtt


 


gagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgcc


 


ccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccag


 


aacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaag


 


gtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatggg


 


tggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctg


 


gacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctg


 


gaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgact


 


gtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatct


 


actggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtac


 


atattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggag


 


cctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccgg


 


tcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggac


 


aacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaat


 


gcaaaaaacgcgctgtgtctgaacatcagctactgcatgacaagggcaagtccatccaagacttgcgccgccgtttcttcctccaccat


 


ctgatcgcggagatccacacagccgaaatcagagctacctcggaggtgtcccccaactccaaacctgctcccaacaccaaaaaccac


 


cccgtgcggtttgggtcagacgatgagggcagatacctaactcaggaaaccaacaaggtggagacgtacaaagaacagccactcaa


 


gacacccgggaagaagaagaaaggcaagcctgggaaacgcagagaacaggagaaaaagaagcgaaggactcggtctgcctggc


 


caagcacagctgcgagtggcctgcttgaggaccccctgccccacacctccaggccctcgctggagcccagcttaaggacgcattga


 


Parathyroid hormone-related protein (PTHrP) Human (nucleic acid sequence):


(SEQ ID NO: 292)



atgcagcggagactggttcagcagtggagcgtcgcggtgttcctgctgagctacgcggtgccctcctgcgggcgctcggtggagggt



 


ctcagccgccgcctcaaaagaTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaactt


 


cctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaa


 


taaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatg


 


gaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccct


 


ggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaag


 


ctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttg


 


ttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgc


 


cccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccag


 


aacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaag


 


gtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatggg


 


tggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctg


 


gacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctg


 


gaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgact


 


gtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatct


 


actggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtac


 


atattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggag


 


cctgttgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatgtctcttcgccgg


 


tcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggac


 


aacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaat


 


gcaaaaaaagagctgtgtctgaacatcagctcctccatgacaaggggaagtccatccaagatttacggcgacgattcttccttcaccatc


 


tgatcgcagaaatccacacagctgaaatcagagctacctcggaggtgtcccctaactccaagccctctcccaacacaaagaaccaccc


 


cgtccgatttgggtctgatgatgagggcagatacctaactcaggaaactaacaaggtggaagacgtacaaagagcagccgctcaagac


 


acctgggaagaaaaagaaaggcaagcccgggaaacgcaaggagcaggaaaagaaaaaacggcgaactcgctctgcctggttaga


 


ctctggagtgactgggagtgggctagaaggggaccacctgtctgacacctccacaacgtcgctggagctcgattcacggaggcattga


 


Osteocalcin Mouse (nucleic acid sequence):


(SEQ ID NO: 293)



atgaggaccatctttctgctcactctgctgaccctggctgcgctctgtctctctgacctcacagatgccaagcccagcggccctgagtctg



 


acaaagccttcatgtccaagcaggagggcaataaggtagtgaacagactccggcgcTactgcgccactgttcattgccaggactgtc


 


cttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgc


 


acgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcag


 


agtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaa


 


ccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactg


 


gaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatctcgccaa


 


cccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtga


 


tcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaa


 


aatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccact


 


ctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctacta


 


ctacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcg


 


ctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtcc


 


ctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaa


 


gtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccg


 


tgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggt


 


ggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaacc


 


aggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacct


 


gaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtc


 


gaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataa


 


agcatggagacaccctagaagtaccagatgaatgcaaacggcgctaccttggagcctcagtccccagcccagatcccctggagcc


 


cacccgggagcagtgtgagcttaaccctgcttgtgacgagctatcagaccagtatggcttgaagaccgcctacaaacgcatctatggtat


 


cactatttag


 


Osteocalcin Human (nucleic acid sequence):


(SEQ ID NO: 294)



atgagagccctcacactcctcgccctattggccctggccgcactttgcatcgctggccaggcaggtgcgaagcccagcggtgcagagt



 


ccagcaaaggtgcagcctttgtgtccaagcaggagggcagcgaggtagtgaagagacccaggcgcTactgcgccactgttcattgc


 


caggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgt


 


ggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaa


 


ttgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgg


 


gtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaa


 


gacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccct


 


atcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttca


 


aactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctg


 


tggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttga


 


cggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccat


 


caacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagg


 


gagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaa


 


ttccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactc


 


ttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggag


 


gagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctg


 


aagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggt


 


aactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaaga


 


acagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagc


 


ctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaaga


 


atgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaggcgctacctgtatcaatggctgggagccccagtc


 


ccctacccggatcccctggagcccaggagggaggtgtgtgagctcaatccggactgtgacgagttggctgaccacatcggctttcagg


 


aggcctatcggcgcttctacggcccggtctag


 


Urocortin-3 Mouse (nucleic acid sequence):


(SEQ ID NO: 295)



atgctgatgcccacctacttcctgctgccacttctgctgctcctaggaggtccaaggacaagcctctcccacaagttctacaacactg



 


gaccagtcttcagctgcctcaacacagccctatctgaggtcaagaagaacaagctggaagatgtgcccttgctgagcaagaaga


 


gctttggccacctgcccacacaagacccctcaggggaagaagatgacaaccaaacgcacctccagatcaaaagaactttctcag


 


gtgccgcgggtgggaatggagctgggagcacccggtacagataccaatcccaggcacagcacaaggggaagctgtacccaga


 


caagcccaaaagcgaccggggcaccaagTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacac


 


agttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggac


 


tgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttaga


 


acattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgt


 


ccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgac


 


atcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcac


 


catcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctg


 


taagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcac


 


ttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgca


 


tactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgccc


 


gctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacct


 


gcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgcc


 


gactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaa


 


caatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaac


 


acttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgc


 


tcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgc


 


tgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtct


 


cttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcct


 


gaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtac


 


cagatgaatgcaaacggggcaccaagttcaccctttcccttgatgttcccactaacatcatgaacatcctcttcaacatcgacaaggcca


 


agaatttgcgagccaaggcagctgccaatgctcagctcatggcacagattgggaagaagaagtaa


 


Urocortin-3 Human (nucleic acid sequence):


(SEQ ID NO: 296)



Atgctgatgccggtccacttcctgctgctcctgctgctgctcctggggggccccaggacaggcctcccccacaagttctacaaagcc



 


aagcccatcttcagctgcctcaacaccgccctgtctgaggctgagaagggccagtgggaggatgcatccctgctgagcaagagg


 


agcttccactacctgcgcagcagagacgcctcttcgggagaggaggaggagggcaaagagaaaaagactttccccatctctggg


 


gccaggggtggagccagaggcacccggtacagatacgtgtcccaagcacagcccaggggaaagccacgccaggacacggcc


 


aagagtccccaccgcTactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgt


 


gaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaa


 


ccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaa


 


gagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggag


 


aacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctact


 


gagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttga


 


gatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgcccc


 


agacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaa


 


caactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtc


 


ttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtgg


 


agacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggac


 


atactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaa


 


cacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtag


 


tagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactg


 


gcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatatt


 


agagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctg


 


tgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaa


 


agtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaaca


 


gggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaa


 


acaccgcaccaagttcaccctgtccctcgacgtccccaccaacatcatgaacctcctcttcaacatcgccaaggccaagaacctgcgtg


 


cccaggcggccgccaatgcccacctgatggcgcaaattgggaggaagaagtag


 


Urocortin-2 Mouse (nucleic acid sequence):


(SEQ ID NO: 297)



Atgatgaccaggtgggcactggtggtgttcgtggtcctgatgttggataggatcctatttgtcccaggaactcctatccccaccttccagc



 


tcctccctcagaactctctggagacaactcctagctctgtgacctcagagagctcctcaggtaccaccacaggaccctcagcttcctgga


 


gcaactctaaagccagcccttacctagacacccgtgtcTactgcgccactgttcattgccaggactgtccttacgaacctgatccacca


 


aacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcag


 


atggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatg


 


gtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactgga


 


aggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatc


 


attgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcga


 


ggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaa


 


gatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacaga


 


cttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgac


 


gttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgcct


 


tcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagagg


 


aacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatgg


 


ccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatca


 


ggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctc


 


agaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagc


 


aactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttt


 


tgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaa


 


tagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtat


 


tgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaa


 


gtaccagatgaatgcaaaacccgtgtcatactctccctggatgttcccattggcctcctacggatcttactggaacaggctcgttacaag


 


gctgccaggaatcaggctgccactaatgctcaaatactagcccatgttggccgccgctga


 


Urocortin-2 Human (nucleic acid sequence):


(SEQ ID NO: 298)



atgaccaggtgtgctctgctgttgctgatggtcctgatgttgggcagagtcctggttgtcccagtgacccctatcccaaccttccagctccg



 


ccctcagaattctccccagaccactccccgacctgcggcctcagagagcccctcagctgctcccacatggccgtgggctgcccagag


 


ccactgcagccccacccgccaccctggctcgcgcattTactgcgccactgttcattgccaggactgtccttacgaacctgatccacca


 


aacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcag


 


atggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatg


 


gtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactgga


 


aggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatc


 


attgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcga


 


ggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaa


 


gatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacaga


 


cttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgac


 


gttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgcct


 


tcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagagg


 


aacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatgg


 


ccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatca


 


ggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctc


 


agaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagc


 


aactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttt


 


tgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaa


 


tagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtat


 


tgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaa


 


gtaccagatgaatgcaaatcgcgcattgtcctatcgctggatgtccccatcggcctcttgcagatcttactggagcaagcccgggccag


 


ggctgccagggagcaggccaccaccaacgcccgcatcctggcccgtgtcggccactgctga


 


Urocortin-1 Mouse (nucleic acid sequence):


(SEQ ID NO: 299)



Atgatacagaggggacgcgctacgctcctggtggcgttgctgctcttggcacagcttcgcccggagagcagccagtggagcccagc



 


ggctgcggcggcaactggggtccaggatccgaatctgcgatggagccctggagtgcggaatcagggcggcggcgtccgcgcgctc


 


ctcttgctgttagcggagcgcttcccgcgccgcgcaggatctgagcctgcgggcgagcggcagcgacggTactgcgccactgttcat


 


tgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcag


 


ctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatg


 


taattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgt


 


tgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgacc


 


aagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgacc


 


ctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttc


 


aaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctct


 


gtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttg


 


acggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaacccca


 


tcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacag


 


ggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagatacca


 


attccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgact


 


cttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttgga


 


ggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacct


 


gaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcgg


 


taactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaaga


 


acagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagc


 


ctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaaga


 


atgctacataaagcatggagacaccctagaagtaccagatgaatgcaaacgacgggacgaccctccactgtccatcgacctcacc


 


ttccacctgctgcggaccctgctggagctagctcggacacagagccagcgcgagcgcgcagagcagaaccgcatcatattcgattcg


 


gtgggcaagtga


 


Urocortin-1 Human (nucleic acid sequence):


(SEQ ID NO: 300)



atgaggcaggcgggacgcgcagcgctgctggccgcgctgctgctcctggtacagctgtgccctgggagcagccagaggagccccg



 


aggcggccggggtccaggacccgagtctgcgctggagccccggggcacggaaccagggtggcggggcccgcgcgctcctcttgc


 


tgctggcggagcgcttcccgcgccgcgcggggcccggccgattgggactcgggacggcaggcgagcggccgcggcggTactgc


 


gccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgt


 


attgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaa


 


tgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctg


 


gtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggg


 


gctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacg


 


gtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccg


 


tcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatg


 


atctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatca


 


accaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagct


 


gccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgc


 


tgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaa


 


agcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcaca


 


caggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaac


 


agattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgacta


 


cagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagact


 


tgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgg


 


gatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaac


 


gtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatggga


 


gttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaacggcgggacaacccttctctgtcc


 


attgacctcacctttcacctgctgcggaccctgctggagctggcgcggacgcagagccagcgggagcgcgccgagcagaaccgcat


 


catattcgactcggtgggcaagtga


 


FGF23 Mouse (nucleic acid sequence):


(SEQ ID NO: 301)



Atgctagggacctgccttagactcctggtgggcgtgctctgcactgtctgcagcttgggcactgctagagcctatccggacacttcccc



 


attgcttggctccaactggggaagcctgacccacctgtacacggctacagccaggaccagctatcacctacagatccatagggatggtc


 


atgtagatggcaccccccatcagaccatctacagtgccctgatgattacatcagaggacgccggctctgtggtgataacaggagccatg


 


actcgaaggttcctttgtatggatctccacggcaacatttttggatcgcttcacttcagcccagagaattgcaagttccgccagtggacgct


 


ggagaatggctatgacgtctacttgtcgcagaagcatcactacctggtgagcctgggccgcgccaagcgcatcttccagccgggcacc


 


aacccgccgcccttctcccagttcctggctcgcaggaacgaggtcccgctgctgcatttctacactgttcgcccacggcgccacacgcg


 


cagctactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaa


 


ggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaaca


 


tgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttcc


 


aggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaa


 


ccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatca


 


ctgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttca


 


acatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaac


 


aaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcag


 


cctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtaca


 


aggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcct


 


cacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatac


 


atttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaa


 


ggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttga


 


tggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacat


 


actgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatg


 


gtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaac


 


ccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcaga


 


aatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccac


 


gcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaacgcagcgccgagg


 


acccaccggagcgcgacccactgaacgtgctcaagccgcggccccgcgccacgcctgtgcctgtatcctgctctcgcgagctgccg


 


agcgcagaggaaggtggccccgcagccagcgatcctctgggggtgctgcgcagaggccgtggagatgctcgcgggggcgcggg


 


aggcgcggataggtgtcgcccctttccaggttcgtctag


 


FGF23 Human (nucleic acid sequence):


(SEQ ID NO: 302)



Atgttgggggcccgcctcaggctctgggtctgtgccttgtgcagcgtctgcagcatgagcgtcctcagagcctatcccaatgcctccc



 


cactgctcggctccagctggggtggcctgatccacctgtacacagccacagccaggaacagctaccacctgcagatccacaaga


 


atggccatgtggatggcgcaccccatcagaccatctacagtgccctgatgatcagatcagaggatgctggctttgtggtgattacag


 


gtgtgatgagcagaagatacctctgcatggatttcagaggcaacatttttggatcacactatttcgacccggagaactgcaggttcca


 


acaccagacgctggaaaacgggtacgacgtctaccactctcctcagtatcacttcctggtcagtctgggccgggcgaagagagcct


 


tcctgccaggcatgaacccacccccgtactcccagttcctgtcccggaggaacgagatccccctaattcacttcaacacccccatac


 


cacggcggcacacccggagctactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaactt


 


cctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaa


 


taaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgatgcagagtagaggccgcaggatggtttagaacattctatg


 


gaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccct


 


ggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaag


 


ctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttg


 


ttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgc


 


cccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccag


 


aacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaag


 


gtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatggg


 


tggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctg


 


gacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctg


 


gaacacttgggatgtgaaggtttcacaaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgact


 


gtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatct


 


actggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtac


 


atattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggag


 


cctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccgg


 


tcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggac


 


aacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaat


 


gcaaacggagcgccgaggacgactcggagcgggaccccctgaacgtgctgaagccccgggcccggatgaccccggccccggcc


 


tcctgttcacaggagctcccgagcgccgaggacaacagcccgatggccagtgacccattaggggtggtcaggggcggtcgagtgaa


 


cacgcacgctggggaacgggcccggaaggctgccgccccttcgccaagttcatctag


 


IL1B Mouse (nucleic acid sequence):


(SEQ ID NO: 303)



Atggcaactgttcctgaactcaactgtgaaatgccaccttttgacagtgatgagaatgacctgttctttgaagttgacggaccccaaaaga



 


tgaagggctgcttccaaacctttgacctgggctgtcctgatgagagcatccagcttcaaatctcgcagcagcacatcaacaagagcttca


 


ggcaggcagtatcactcattgtggctgtggagaagctgtggcagctacctgtgtctttcccgtggaccttccaggatgaggacatgagca


 


ccttcttttccttcatctttgaagaagagcccatcctctgtgactcatgggatgatgatgataacctgctggtgtgtgacgttccctactgcgc


 


cactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtatt


 


gatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgt


 


gtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggta


 


catacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggct


 


gtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaacggtg


 


gagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtca


 


ttgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatc


 


tctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaacc


 


aggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgcc


 


gcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgct


 


tgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagc


 


aagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacag


 


gaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacaga


 


ttctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacag


 


ccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgc


 


ggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggat


 


gcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtg


 


tgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagtt


 


caagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaactggtgtgtgacgttcccgttcccatta


 


gacaactgcactacaggctccgagatgaacaacaaaaaagcctcgtgctgtcggacccatatgagctgaaagctctccacctcaatgg


 


acagaatatcaaccaacaagtgatattctccatgagctttgtacaaggagaaccaagcaacgacaaaatacctgtggccttgggcctcaa


 


aggaaagaatctatacctgtcctgtgtaatgaaagacggcacacccaccctgcagctggagagtgtggatcccaagcaatacccaaag


 


aagaagatggaaaaacggtttgtcttcaacaagatagaagtcaagagcaaagtggagtttgagtctgcagagttccccaactggtacatc


 


agcacctcacaagcagagcacaagcctgtcttcctgggaaacaacagtggtcaggacataattgacttcaccatggaatccgtgtcttcc


 


taa


 


IL1B Human (nucleic acid sequence):


(SEQ ID NO: 304)



Atggcagaagtacctgagctcgccagtgaaatgatggcttattacagtggcaatgaggatgacttgttctttgaagctgatggccctaaa



 


cagatgaagtgctccttccaggacctggacctctgccctctggatggcggcatccagctacgaatctccgaccaccactacagcaagg


 


gcttcaggcaggccgcgtcagttgttgtggccatggacaagctgaggaagatgctggttccctgcccacagaccttccaggagaatga


 


cctgagcaccttctttcccttcatctttgaagaagaacctatcttcttcgacacatgggataacgaggcttatgtgcacgatgcaccttactg


 


cgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatg


 


tattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccga


 


atgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacct


 


ggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaaggg


 


ggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaac


 


ggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcacc


 


gtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaat


 


gatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatc


 


aaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagc


 


tgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtg


 


ctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgaca


 


aagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcac


 


acaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaa


 


cagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgact


 


acagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaaga


 


cttgcggtattttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccg


 


ggatgcaccgaagaacagaaacctgaagctgaacgactcgtcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgta


 


acgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgg


 


gagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaTatgtgcacgatgcacctgta


 


cgatcactgaactgcacgctccgggactcacagcaaaaaagcttggtgatgtctggtccatatgaactgaaagctctccacctccaggg


 


acaggatatggagcaacaagtggtgttctccatgtcctttgtacaaggagaagaaagtaatgacaaaatacctgtggccttgggcctcaa


 


ggaaaagaatctgtacctgtcctgcgtgttgaaagtgataagcccactctacagctggagagtgtagatcccaaaaattacccaaagaa


 


gaagatggaaaagcgatttgtcttcaacaagatagaaatcaataacaagctggaatttgagtctgcccagttccccaactggtacatcag


 


cacctctcaagcagaaaacatgcccgtcttcctgggagggaccaaaggcggccaggatataactgacttcaccatgcaatttgtgtcttc


 


ctaa


 


TNFA Mouse (nucleic acid sequence):


(SEQ ID NO: 305)



Atgagcacagaaagcatgatccgcgacgtggaactggcagaagaggcactcccccaaaagatggggggcttccagaactccaggc



 


ggtgcctatgtctcagcctcttctcattcctgcttgtggcaggggccaccacgctcttctgtctactgaacttcggggtgatcggtccccaa


 


agggatgagaagttcccaaatggcctccctctcatcagttctatggcccagaccctcacactcagatactgcgccactgttcattgccag


 


gactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggc


 


acctgcacgagacatactatcagatggactgtgtgaaaataaacccaggaaaaacatgttgccgaatgtgtcagtatgtaattga


 


atgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtc


 


aaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagac


 


aagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatca


 


tcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaact


 


gatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtgga


 


gatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggt


 


tgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaac


 


ttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggag


 


acgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattcc


 


agggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttac


 


actgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggag


 


aagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaag


 


ctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaact


 


acaaccaggatttcagtgatgattcttttgatgctgaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacag


 


aaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgac


 


cgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgcta


 


cataaagcatggagacaccctagaagtaccagatgaatgcaaactcagatcatcttctcaaaattcgagtgacaagcctgtagccca


 


cgtcgtagcaaaccaccaagtggaggagcagctggagtggctgagccagcgcgccaacgccctcctggccaacggcatggatctca


 


aagacaaccaactagtggtgccagccgatgggttgtaccttgtctactcccaggttctcttcaagggacaaggctgccccgactacgtgc


 


tcctcacccacaccgtcagccgatttgctatctcataccaggagaaagtcaacctcctctctgccgtcaagagcccctgccccaaggac


 


acccctgagggggctgagctcaaaccctggtatgagcccatatacctgggaggagtcttccagctggagaagggggaccaactcagc


 


gctgaggtcaatctgcccaagtacttagactttgcggagtccgggcaggtctactttggagtcattgctctgtga


 


TNFA Human (nucleic acid sequence):


(SEQ ID NO: 306)



Atgagcactgaaagcatgatccgggacgtggagctggccgaggaggcgctccccaagaagacaggggggccccagggctccag



 


gcggtgcttgttcctcagcctcttctccttcctgatcgtggcaggcgccaccacgctcttctgcctgctgcactttggagtgatcggccccc


 


agagggaagagttccccagggacctctctctaatcagccctctggcccaggcagtcagatactgcgccactgttcattgccaggactgt


 


ccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctg


 


cacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgca


 


gagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaagga


 


accaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagact


 


ggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgcca


 


acccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtg


 


atcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatctta


 


aaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccac


 


tctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctacta


 


ctacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcg


 


ctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtcc


 


ctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaa


 


gtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccg


 


tgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggt


 


ggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaacc


 


aggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacct


 


gaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtc


 


gaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataa


 


agcatggagacaccctagaagtaccagatgaatgcaaagtcagatcatcttctcgaaccccgagtgacaagcctgtagcccatgttg


 


tagcaaaccctcaagctgaggggcagctccagtggctgaaccgccgggccaatgccctcctggccaatggcgtggagctgagagat


 


aaccagctggtggtgccatcagagggcctgtacctcatctactcccaggtcctcttcaagggccaaggctgcccctccacccatgtgct


 


cctcacccacaccatcagccgcatcgccgtctcctaccagaccaaggtcaacctcctctctgccatcaagagcccctgccagagggag


 


accccagagggggctgaggccaagccctggtatgagcccatctatctgggaggggtcttccagctggagaagggtgaccgactcag


 


cgctgagatcaatcggcccgactatctcgactttgccgagtctgggcaggtctactttgggatcattgccctgtga


 


IFNG Mouse (nucleic acid sequence):


(SEQ ID NO: 307)



Atgaacgctacacactgcatcttggctttgcagctcttcctcatggctgtttctggctgttactgccacggcacagtcattgaaagcctaga



 


aagtctgaataactattttaactcaagtggcatagatgtggaagaaaagagtctcttcttggatatctggaggaactggcaaaaggatggt


 


gacatgaaaatcctgcagagccagattatctctttctacctcagactctttgaagtcttgaaagacaatcaggccatcagcaacaacataa


 


gcgtcattgaatcacacctgattactaccttcttcagcaacagcaaggcgaaaaaggatgcattcatgagtattgccaagtttgaggtcaa


 


caacccacaggtccagcgccaagcattcaatgagctcatccgagtggtccaccagctgttgccggaatccagcctcaggaagtactgc


 


gccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgt


 


attgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaa


 


tgtgtcagtatgtaattgatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctg


 


gtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggg


 


gcgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacg


 


gtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccg


 


tcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatg


 


atctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatca


 


accaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagct


 


gccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgc


 


tgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaa


 


agcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcaca


 


caggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaac


 


agattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgacta


 


cagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagact


 


tgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgg


 


gatgcaccgaagacagaaacctgaagctgaacgactctgcaatagtctcttgccggtcaaagtgatcttgatcagaaatgtaac


 


gtgtccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatggga


 


gttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaacggaaaaggagtcgctgctga


 


IFNG Human (nucleic acid sequence):


(SEQ ID NO: 308)



Atgaaatatacaagttatatcttggcttttcagctctgcatcgttttgggttctcttggctgttactgccaggacccatatgtaaaagaag



 


cagaaaaaccttaagaaatattttaatgcaggtcattcagatgtagcggataatggaactcttttcttaggcattttgaagaattggaaa


 


gaggagagtgacagaaaaataatgcagagccaaattgtctccttttacttcaaactttttaaaaactttaaagatgaccagagcatc


 


caaagagtgtggagaccatcaaggaagacatgaatgtcaagtttttcaatagcaacaaaagaaacgagatgacttcgaaaa


 


gctgactaattattcggtaactgacttgaatgtccaacgcaaagcaatacatgaactcatccaagtgatggctgaactgtcgccagc


 


agctaaaacagggaagcgaaaaaggagtcagatgctgtttcgaggtcgaagatactgcgccactgttcattgccaggactgtcctt


 


acgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcac


 


gagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcaga


 


gtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaac


 


caagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactg


 


gaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaa


 


cccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacataccgtcattgagttcttcaaactgatcgtga


 


tcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaa


 


aatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccact


 


ctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctacta


 


ctacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcg


 


ctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtcc


 


ctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaa


 


gtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccg


 


tgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggt


 


ggtcaagttcaacttcaagcaactgctcgtcgtacattagagatccattcgatggtaagacttgcggtatttgcggtaactacaacc


 


aggatttcagtgatgattcttttgtgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacct


 


gaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtc


 


gaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtaccacgcatgggagttcaagaaagaatgctacataa


 


agcatggagacaccctagaagtaccagatgaatgcaaaggtcgaagagcatcccagtaa


 


Sortilin Mouse (nucleic acid sequence):


(SEQ ID NO: 309)



Atggagcggccccggggagctgcggacggccttttgcgctggcccctcggcctcctcctgctccttcaactgctgcctcctgccgccg



 


tcggccaggaccggctggacgcgccgccgccgcccgcgcctcctctgctgcgctgggccggtccggtcggggtgagctgggggct


 


gcgcgccgccgcgcccgggggccccgtcccccgcgctggccgttggcgccgctactgcgccactgttcattgccaggactgtcctt


 


acgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcac


 


gagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcaga


 


gtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtactacgtgttgggtcaaggaac


 


caagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactg


 


gaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaa


 


cccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtga


 


tcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaa


 


aatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacgttgtccact


 


ctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctacta


 


ctacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcg


 


ctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtcc


 


ctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaa


 


gtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccg


 


tgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggt


 


ggtcaagttcaacttcagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaacc


 


aggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacct


 


gaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtc


 


gaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataa


 


agcatggagacaccctagaagtaccagatgaatgcaaacgccgcggcgcgcccgccgaggaccaagactgcggccgcctccc


 


ggacttcatcgccaagctgaccaacaatacgcaccagcatgtctttgatgacctcagtggctcagtgtccttgtcctgggttggagacag


 


cactggggttattctcgtcctgaccactttccaagtgcctctggtaattgtgagctttggacagtccaagttgtatcgaagtgaggattatgg


 


aaagaactttaaggatattacaaatctcatcaataacaccttcattcggacggaatttggcatggctattggtcctgagaactctggaaagg


 


tgatactaacagcggaggtgtccgggggaagccgaggcggaagagtgttcaggtcatcagactttgccaagaactttgtgcaaacaga


 


tctcccctttcatcctctgacgcagatgatgtacagccctcagaattctgattacctgttagctctcagcaccgaaaatggcctgtgggtgtc


 


caagaattttggggaaaaatgggaagaaatccacaaagcagtatgtttggccaaatggggaccaaacaacatcatcttctttaccaccca


 


tgtgaatggctcctgcaaagctgatcttggtgccctggaattatggagaacatccgacttgggaaaaaccttcaaaaccattggtgtgaaa


 


atctactcctttggtcttgggggccgtttcctttttgcctctgtgatggctgataaggacacaacaagaaggatccatgtgtcaacagacca


 


gggggacacatggagcatggcacaacttccttctgtgggacaggaacagttctactccatcctggcagccaatgaggacatggtcttca


 


tgcatgtagatgaacctggagataccgggtttggcaccaatctttacctctgatgatcgaggcattgtctactccaagtctctggacagacat


 


ctctataccaccacaggcggggagacggactttaccaacgtgacttccctccgtggggtctatataacaagcacgctctcagaagataa


 


ctctattcagagcatgatcactttgaccagggaggacggtgggagcacctgcggaagccggagaacagcaagtgcgacgctaccgc


 


aaagaacaagaacgagtgcagccttcatatccatgcttcttatagcatctcccagaagctaaacgttccaatggccccactttccgagccc


 


aatgctgtgggcatagtcatcgctcacggtagtgtgggagatgccatctcggtgatggtcccagatgtgtacatctcagatgatgggggt


 


tactcctgggcgaagatgctagaaggaccacattactataccatcctggactctggaggcatcattgtggccattgagcacagcaaccgt


 


cctatcaatgtgattaagttctccacagatgaaggccagtgctggcagagctatgtgttcacacaggagcccatctacttcactgggcttg


 


cttccgagcctggagccaggtccatgaacatcagcatctggggattcacagagtctttcattacccgccagtgggtctcctacacagtcg


 


atttcaaagacatccttgagcggaattgtgaagaggatgactataccacgtggctggcacactccacagaccctggagattacaaagac


 


ggctgcattttgggctataaagaacagttcctacggctacggaagtcatccgtctgtcagaatggtcgagactatgttgtggccaagcag


 


ccatccgtctgtccgtgttccctggaggacttcctctgtgactttggctacttccgtccggagaacgcctcagagtgcgtggagcagcctg


 


aactgaaggggcatgagttagagttctgtctgtacggcaaggaggagcacctgacaacaaatgggtaccggaaaatcccaggagaca


 


aatgccaaggtgggatgaatcccgccagagaagtaaaagacttgaaaaagaaatgcacaagcaacttcttgaaccccacaaagcagg


 


actcccgcccacagggacacagcttgtcccagaatccagctccgcctcctcttggatacactgaaaacacacacttcctatctcctaccc


 


agaagcagaattccaagtcaaattctgtccctattatcctggccatcgtgggactgatgcttgtcacagtcgtagcaggagtcctcattgtg


 


aagaaatatgtctgtggcggaaggttcctggtgcaccggtactcggtgctacagcagcacgcagaggctgacggcgtagaggctttgg


 


attcaacctcccacgctaaaagcggatatcacgacgactcagatgaggacctcctggaatag


 


Sortilin Human (nucleic acid sequence):


(SEQ ID NO: 310)



Atggagcggccctggggagctgcggacggcctctcgcgctggccccatggcctcggcctcctcctcctcctgcagctgctgccgcc



 


gtcgaccctcagccaggaccggctggacgcgccgccgccgcccgctgcgccgctgccgcgctggtctggccccatcggggtgagc


 


tgggggctgcgggcggccgcagccgggggcgcgtttccccgcggcggccgttggcgtcgctactgcgccactgttcattgccagg


 


actgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggca


 


cctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaa


 


tgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtca


 


aggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagaca


 


agactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcat


 


cgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactg


 


atcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggag


 


atcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggtt


 


gtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaact


 


tctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggaga


 


cgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattcca


 


gggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttaca


 


ctgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggaga


 


agccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagct


 


ctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaacta


 


caaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacaga


 


aacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgacc


 


gtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctac


 


ataaagcatggagacaccctagaagtaccagatgaatgcaaacgtcgcagcgcgccgggcgaggacgaggagtgcggccggg


 


tccgggacttcgtcgccaagctggccaacaacacgcaccagcatgtgtttgatgatctcagaggctcagtatccttgtcctgggttggag


 


atagcactggggtcattctagtcttgactaccttccatgtaccactggtaattatgacttttggacagtccaagctatatcgaagtgaggatta


 


tgggaagaactttaaggatattacagatctcatcaataacacctttattcggactgaatttggcatggctattggtcctgagaactctggaaa


 


ggtggtgttaacagcagaggtgtctggaggaagtcgtggaggaagaatctttagatcatcagattttgcgaagaattttgtgcaaacag at


 


ctcccttttcatcctctcactcagatgatgtatagccctcagaattctgattatcttttagctctcagcactgaaaatggcctgtgggtgtccaa


 


gaattttgggggaaaatgggaagaaatccacaaagcagtatgtttggccaaatggggatcagacaacaccatcttctttacaacctatgc


 


aaatggctcctgcaaagctgaccttggggctctggaattatggagaacttcagacttgggaaaaagcttcaaaactattggtgtgaaaatc


 


tactcatttggtcttgggggacgtttcctttttgcctctgtgatggctgataaggatacaacaagaaggatccacgtttcaacagatcaagg


 


ggacacatggagcatggcccagctcccctccgtgggacaggaacagttctattctattctggcagcaaatgatgacatggtattcatgca


 


tgtagatgaacctggagacactgggtttggcacaatctttacctcagatgatcgaggcattgtctattccaagtctttggaccgacatctcta


 


cactaccacaggcggagagacggactttaccaacgtgacctccctccgcggcgtctacataacaagcgtgctctccgaagataattcta


 


tccagaccatgatcacttttgaccaaggaggaaggtggacgcacctgaggaagcctgaaaacagtgaatgtgatgctacagcaaaaaa


 


caagaatgagtgcagccttcatattcatgcttcctacagcatctcccagaaactgaatgttccaatggccccactctcagagccgaatgcc


 


gtaggcattgtcattgctcatggtagcgtgggggatgccatctcagtgatggttccagatgtgtacatctcagatgatgggggttactcctg


 


gacaaagatgctggaaggaccccactattacaccatcctggattctggaggcatcattgtggccattgagcacagcagccgtcctatca


 


atgtgattaagttctccacagacgaaggtcaatgctggcaaacctacacgttcaccagggaccccatctatttcactggcctagcttcaga


 


acctggagctaggtccatgaatatcagcatttggggcttcacagaatctttcctgaccagccagtgggtctcctacaccattgattttaaag


 


atatccttgaaaggaactgtgaagagaaggactataccatatggctggcacactccacagaccctgaagattatgaagatggctgcatttt


 


gggctacaaagaacagtttctgcggctacgcaagtcatccgtgtgtcagaatggtcgagactatgttgtgaccaagcagccctccatctg


 


cctctgttccctggaggactttctctgtgattttggctactaccgtccagaaaatgactccaagtgtgtggaacagccagaactgaagggc


 


cacgacctggagttttgtctgtacggaagagaagaacacctaacaacaaatgggtaccggaaaattccaggggacaaatgccagggt


 


ggggtaaatccagttcgagaagtaaaagacttgaaaaagaaatgcacaagcaactttttgagtccggaaaaacagaattccaagtcaaa


 


ttctgttccaattatcctggccatcgtgggattgatgctggtcacagtcgtagcaggagtgctcattgtgaagaaatatgtctgtgggggaa


 


ggttcctggtgcatcgatactctgtgctgcagcagcatgcagaggccaatggtgtggatggtgtggatgctttggacacagcctcccaca


 


ctaataaaagtggttatcatgatgactcagatgaggacctcttggaatag


 


Neuropeptide W Mouse (nucleic acid sequence):


(SEQ ID NO: 311)



Ctggcgtctaacagagaagtgcggggccctgggcccgggactcccaggaaccggcccctgctgcccctgctgctgcttctgctcttg



 


ctaccgctgcccgccagcgcctggtataagcacgtggcgagtccccgctatcacacagtgggtcgtgcctccgggctgctcatgggg


 


ctgcgccgctcgccctaccagtggcgccgttactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacaca


 


gttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggact


 


gtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttaga


 


acattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgt


 


ccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgac


 


atcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcac


 


catcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctg


 


taagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcac


 


ttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgca


 


tactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgccc


 


gctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacct


 


gcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgcc


 


gactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaa


 


caatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaac


 


acttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgc


 


tcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgc


 


tgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtct


 


cttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcct


 


gaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtac


 


cagatgaatgcaaacgccgtgccctgggcggggctgctggacccctctcccggctcccaggaccggtcgcccgcggcgctctcct


 


gcttccttcctcagggcaggagctgtgggaggtacgaagcaggagctcacctgcagggcttcccgtccatgcaccctggagtccgcg


 


ggacctggagggagtccgccaaccggagcagtcgctaagccttcactcctggatctcagaggagcccgctgctagagccttcggaga


 


gacgcttcgtgcccagccatggttcctgcagcaagtcatctttgccgatcctgtcaggcccaagaaccgatggcgcccccatgcttga


 


Neuropeptide W Human (nucleotide acid sequence):


(SEQ ID NO: 312)



Ctggcgtggcgcccaggggagcggggggctcccgcgagccggccgcggctggcactgctgctgcttctgctcctgctgccgctgc



 


cctccggcgcgtggtacaagcacgtggcgagtccccgctaccacacggtgggccgcgccgctggcctgctcatggggctgcgtcgc


 


tcacctatctgtggcgccgctactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttc


 


ctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaat


 


aaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatgg


 


aaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctg


 


gagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagct


 


actgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgtt


 


gagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgcc


 


ccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccag


 


aacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaag


 


gtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctctgcgccttcgcccgctgtatggg


 


tggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctg


 


gacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctg


 


gaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgact


 


gtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatct


 


actggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtac


 


atattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggag


 


cctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccgg


 


tcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggac


 


aacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaat


 


gcaaacgccgcgcgctgcgcgcggccgccgggcccctggccagggacaccctctcccccgaacccgcagcccgcgaggctcctc


 


tcctgctgccctcgtgggttcaggagctgtgggagacgcgacgcaggagctcccaggcagggatccccgtccgtgcgccccggagc


 


ccgcgcgccccagagcctgcgctggaaccggagtccctggacttcagcggagctggccagagacttcggagagacgtctcccgccc


 


agcggtggaccccgcagcaaaccgccttggcctgccctgcctggcccccggaccgttctga


 


CART Mouse (nucleic acid sequence):


(SEQ ID NO: 313)



Atggagagctcccgcctgcggctgctacccctcctgggcgccgccctgctgctactgctacctttgctgggtgcccgtgcccaggagg



 


acgccgagctgcagccccgagccctggacatctactctgccgtggatgatgcgtcccacgagaaggagctgatcgaagcgttgcaag


 


aagtcctgaagaagctcaagagtaaacgctactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagt


 


tccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgt


 


gtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaac


 


attctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtcc


 


atcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatc


 


gctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccat


 


cgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaa


 


gaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttca


 


gatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatact


 


gcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgct


 


gtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcg


 


ttttgtctggacatactttctaacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgac


 


tgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaa


 


tcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacactt


 


ccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgt


 


cgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggaatttcagtgatgattcttttgatgctga


 


aggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttc


 


gccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgag


 


gggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccag


 


atgaatgcaaaaaacgcattccgatctacgagaagaagtacggccaagtccccatgtgtgacgctggagagcagtgcgcagtgagg


 


aaaggggccaggatcgggaagctgtgtgactgtccccgaggaacttcctgcaattctttcctcttgaagtgcttgtga


 


CART Human (nucleic acid sequence):


(SEQ ID NO: 314)



Atggagagctcccgcgtgaggctgctgcccctcctgggcgccgccctgctgctgatgctacctctgttgggtacccgtgcccaggag



 


gacgccgagctccagccccgagccctggacatctactctgccgtggatgatgcctcccacgagaaggagctgatcgaagcgctgcaa


 


gaagtcttgaagaagctcaagagtaaacgttactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacag


 


ttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgt


 


gtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaac


 


attctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtcc


 


atcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatc


 


gctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccat


 


cgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaa


 


gaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttca


 


gatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatact


 


gcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgct


 


gtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcg


 


ttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgac


 


tgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaa


 


tcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacactt


 


ccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgt


 


cgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctga


 


aggagcctgtgatctgacccccaacccaccgggatgcaccgaagacagaaacctgaagctgaacgactctgcaatagtctcttc


 


gccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgag


 


gggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccag


 


atgaatgcaaaaaacgtgttcccatctatgagaagaagtatggccaagtccccatgtgtgacgccggtgagcagtgtgcagtgaggaa


 


aggggcaaggatcgggaagctgtgtgactgtccccgaggaacctcctgcaattccttcctcctgaagtgcttatga


 


TGFB1 Mouse (nucleic acid sequence):


(SEQ ID NO: 315)



Atgccgccctcggggctgcggctactgccgcttctgctcccactcccgtggcttctagtgctgacgcccgggaggccagccgcggga



 


ctctccacctgcaagaccatcgacatggagctggtgaaacggaagcgcatcgaagccatccgtggccagatcctgtccaaactaagg


 


ctcgccagtcccccaagccagggggaggtaccgcccggcccgctgcccgaggcggtgctcgctttgtacaacagcacccgcgacc


 


gggtggcaggcgagagcgccgacccagagccggagcccgaagcggactactatgctaaagaggtcacccgcgtgctaatggtgga


 


ccgcaacaacgccatctatgagaaaaccaaagacatctcacacagtatatatatgttcttcaatacgtcagacattcgggaagcagtgcc


 


cgaacccccattgctgtcccgtgcagagctgcgcttgcagagattaaaatcaagtgtggagcaacatgtggaactctaccagaaatata


 


gcaacaattcctggcgttaccttggtaaccggctgctgacccccactgatacgcctgagtggctgtcttttgacgtcactggagttgtacg


 


gcagtggctgaaccaaggagacggaatacagggctttcgattcagcgctcactgctcttgtgacagcaaagataacaaactccacgtg


 


gaaatcaacgggatcagccccaaacgtcggggcgacctgggcaccatccatgacatgaaccggcccttcctgctcctcatggccacc


 


cccctggaaagggcccagcacctgcacagctcacggcaccggagatactgcgccactgttcattgccaggactgtccttacgaacct


 


gatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagaca


 


tactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggcc


 


gcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcg


 


gcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggct


 


ggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacac


 


catcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcc


 


tcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatgga


 


agatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaat


 


cctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatct


 


cctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaa


 


actagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggag


 


attcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagt


 


acgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgt


 


acagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaa


 


cttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtg


 


atgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacg


 


actctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatg


 


tacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagaca


 


ccctagaagtaccagatgaatgcaaacggagagccctggataccaactattgcttcagctccacagagaagaactgctgtgtgcggc


 


agctgtacattgactttaggaaggacctgggttggaagtggatccacgagcccaagggctaccatgccaacttctgtctgggaccctgc


 


ccctatatttggagcctggacacacagtacagcaaggtccttgccctctacaaccaacacaacccgggcgcttcggcgtcaccgtgctg


 


cgtgccgcaggctttggagccactgcccatcgtctactacgtgggtcgcaagcccaaggtggagcagttgtccaacatgattgtgcgct


 


cctgcaagtgcagctga


 


TGFB1 Human (nucleic acid sequence):


(SEQ ID NO: 316)



Atgccgccctccgggctgcggctgctgccgctgctgctaccgctgctgtggctactggtgctgacgcctggccggccggccgcggg



 


actatccacctgcaagactatcgacatggagctggtgaagcggaagcgcatcgaggccatccgcggccagatcctgtccaagctgcg


 


gctcgccagccccccgagccagggggaggtgccgcccggcccgctgcccgaggccgtgctcgccctgtacaacagcacccgcga


 


ccgggtggccggggagagtgcagaaccggagcccgagcctgaggccgactactacgccaaggaggtcacccgcgtgctaatggtg


 


gaaacccacaacgaaatctatgacaagttcaagcagagtacacacagcatatatatgttcttcaacacatcagagctccgagaagcggt


 


acctgaacccgtgttgctctcccgggcagagctgcgtctgctgaggctcaagttaaaagtggagcagcacgtggagctgtaccagaaa


 


tacagcaacaattcctggcgatacctcagcaaccggctgctggcacccagcgactcgccagagtggttatcttttgatgtcaccggagtt


 


gtgcggcagtggttgagccgtggaggggaaattgagggctttcgccttagcgcccactgctcctgtgacagcagggataacacactgc


 


aagtggacatcaacgggttcactaccggccgccgaggtgacctggccaccattcatggcatgaaccggcctttcctgcttctcatggcc


 


accccgctggagagggcccagcatctgcaaagctcccggcaccgccgatactgcgccactgttcattgccaggactgtccttacgaa


 


cctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagag


 


acatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagag


 


gccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaaggg


 


cggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtgg


 


ctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtac


 


accatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacat


 


cctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatg


 


gaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatgga


 


aatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacacc


 


atctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctccc


 


gaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaag


 


gagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagaga


 


aagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtc


 


ccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagt


 


tcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttc


 


agtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctg


 


aacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgat


 


gcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatgg


 


agacaccctagaagtaccagatgaatgcaaacgccgagccctggacaccaactattgcttcagctccacggagaagaactgctgcg


 


tgcggcagctgtacattgacttccgcaaggacctcggctggaagtggatccacgagcccaagggctaccatgccaacttctgcctcgg


 


gccctgcccctacatttggagcctggacacgcagtacagcaaggtcctggccctgtacaaccagcataacccgggcgcctcggcggc


 


gccgtgctgcgtgccgcaggcgctggagccgctgcccatcgtgtactacgtgggccgcaagcccaaggtggagcagctgtccaaca


 


tgatcgtgcgctcctgcaagtgcagctga


 


TGFB2 Mouse (nucleic acid sequence):


(SEQ ID NO: 317)



Atgcactactgtgtgctgagcacctttttgctcctgcatctggtcccggtggcgctcagtctgtctacctgcagcaccctcgacatggatc



 


agtttatgcgcaagaggatcgaggccatccgcgggcagatcctgagcaagctgaagctcaccagccccccggaagactatccggag


 


ccggatgaggtccccccggaggtgatttccatctacaacagtaccagggacttactgcaggagaaggcaagccggagggcagccgc


 


ctgcgagcgcgagcggagcgacgaggagtactacgccaaggaggtttataaaatcgacatgccgtcccacctcccctccgaaaatgc


 


catcccgcccactttctacagaccctacttcagaatcgtccgctttgatgtctcaacaatggagaaaaatgcttcgaatctggtgaaggca


 


gagttcagggtcttccgcttgcaaaaccccaaagccagagtggccgagcagcggattgaactgtatcagatccttaaatccaaagactt


 


aacatctcccacccagcgctacatcgatagcaaggttgtgaaaaccagagcggagggtgaatggctctccttcgacgtgacagacgct


 


gtgcaggagtggcttcaccacaaagacaggaacctggggtttaaaataagtttacactgcccctgctgtaccttcgtgccgtctaataatt


 


acatcatcccgaataaaagcgaagagctcgaggcgagatttgcaggtattgatggcacctctacatatgccagtggtgatcagaaaact


 


ataaagtccactaggaaaaaaaccagtgggaagaccccacatctcctgctaatgttgttgccctcctacagactggagtcacaacagtcc


 


agccggcggtactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagcta


 


aagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaa


 


aaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattcc


 


agttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctgg


 


atggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatc


 


ccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccag


 


gcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacag


 


caaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgct


 


attcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagc


 


cgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagc


 


gagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttcta


 


cgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttggga


 


tgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactca


 


ttgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatg


 


gtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatcc


 


attcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgac


 


ccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatctt


 


gatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctg


 


tgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaacggcgga


 


agaagcgcgctttggatgctgcctactgctttagaaatgtgcaggataattgctgccttcgccctctttacattgattttaagagggatcttgg


 


atggaaatggatccatgaacccaaagggtacaatgctaacttctgtgctggggcatgcccatatctatggagttcagacactcaacacac


 


caaagtcctcagcctgtacaacaccataaatcccgaagcttccgcttccccttgctgtgtgtcccaggatctggaaccactgaccattctct


 


attacattggaaatacgcccaagatcgaacagctttccaatatgattgtcaagtcttgtaaatgcagctaa


 


TGFB2 Human (nucleic acid sequence):


(SEQ ID NO: 318)



Atgcactactgtgtgctgagcgcttttctgatcctgcatctggtcacggtcgcgctcagcctgtctacctgcagcacactcgatatggacc



 


agttcatgcgcaagaggatcgaggcgatccgcgggcagatcctgagcaagctgaagctcaccagtcccccagaagactatcctgagc


 


ccgaggaagtccccccggaggtgatttccatctacaacagcaccagggacttgctccaggagaaggcgagccggagggcggccgc


 


ctgcgagcgcgagaggagcgacgaagagtactacgccaaggaggtttacaaaatagacatgccgcccttcttcccctccgaaactgtc


 


tgcccagttgttacaacaccctctggctcagtgggcagcttgtgctccagacagtcccaggtgctctgtgggtaccttgatgccatcccgc


 


ccactttctacagaccctacttcagaattgttcgatttgacgtctcagcaatggagaagaatgcttccaatttggtgaaagcagagttcaga


 


gtctttcgtagcagaacccaaaagccagagtgcctgaacaacggattgagctatatcagattctcaagtccaaagatttaacatctccaac


 


ccagcgctacatcgacagcaaagttgtgaaaacaagagcagaaggcgaatggctctccttcgatgtaactgatgctgttcatgaatggct


 


tcaccataaagacaggaacctgggatttaaaataagcttacactgtccctgctgcacttttgtaccatctaataattacatcatcccaaataa


 


aagtgaagaactagaagcaagatttgcaggtattgatggcacctccacatataccagtggtgatcagaaaactataaagtccactaggaa


 


aaaaaacagtgggaagaccccacatctcctgctaatgttattgccctcctacagacttgagtcacaacagaccaaccggcggtactgcg


 


ccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgta


 


ttgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaat


 


gtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctgg


 


tacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaaggggg


 


ctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggt


 


ggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtc


 


attgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatga


 


tctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaa


 


ccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctg


 


ccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgct


 


gcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaa


 


gcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacac


 


aggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaaca


 


gattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactac


 


agccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagactt


 


gcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccggg


 


atgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacg


 


tgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggag


 


ttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaacggcggaagaagcgtgctttggat


 


gcggcctattgctttagaaatgtgcaggataattgctgcctacgtccactttacattgatttcaagagggatctagggtggaaatggataca


 


cgaacccaaagggtacaatgccaacttctgtgctggagcatgcccgtatttatggagttcagacactcagcacagcagggtcctgagctt


 


atataataccataaatccagaagcatctgcttctccttgctgcgtgtcccaagatttagaacctctaaccattctctactacattggcaaaaca


 


cccaagattgaacagctttctaatatgattgtaaagtcttgcaaatgcagctaa


 


TGFB3 Mouse (nucleic acid sequence):


(SEQ ID NO: 319)



Atgaagatgcacttgcaaagggctctggtagtcctggccctgctgaacttggccacaatcagcctctctctgtccacttgcaccacgttg



 


gacttcggccacatcaagaagaagagggtggaagccattaggggacagatcttgagcaagctcaggctcaccagcccccctgagcc


 


atcggtgatgacccacgtcccctatcaggtcctggcactttacaacagcacccgggagttgctggaagagatgcacggggagaggga


 


ggaaggctgcactcaggagacctcggagtctgagtactatgccaaagagatccataaattcgacatgatccagggactggcggagca


 


caatgaactggccgtctgccccaaaggaattacctctaaggtttttcgtttcaatgtgtcctcagtggagaaaaatggaaccaatctgttcc


 


gggcagagttccgggtcttgcgggtgcccaaccccagctccaagcgcacagagcagagaattgagctcttccagatacttcgaccgg


 


atgagcacatagccaagcagcgctacataggtggcaagaatctgcccacaaggggcaccgctgaatggctgtctttcgatgtcactga


 


cactgtgcgcgagtggctgttgaggagagagtccaacttgggtctggaaatcagcatccactgtccatgtcacacctttcagcccaatgg


 


agacatactggaaaatgttcatgaggtgatggaaatcaaattcaaaggagtggacaatgaagatgaccatggccgtggagacctgggg


 


cgtctcaagaagcaaaaggatcaccacaacccacacctgatcctcatgatgatccccccacaccgactggacagcccaggccagggc


 


agtcagaggaagtactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaag


 


ctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccag


 


gaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagaga


 


ttccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacc


 


tggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgaga


 


atcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgc


 


caggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagaca


 


cagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactc


 


gctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctgga


 


gccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacga


 


gcgagcctcacagtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttc


 


tacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgg


 


gatgtgaaggtttcacacaggaatgttgactcttacaactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaact


 


cattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaaga


 


tggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatc


 


cattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctga


 


cccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatct


 


tgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttct


 


gtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccgatgaatgcaaaaggaag


 


aagagggccctggacaccaattactgcttccgcaacctggaggagaactgctgtgtacgccccctttatattgacttccggcaggatcta


 


ggctggaaaatgggtccacgaacctaagggttactatgccaacttctgctcaggcccttgcccatacctccgcagcgcagacacaaccc


 


atagcacggtgcttggactatacaacaccctgaacccagaggcgtctgcctcgccatgctgcgtcccccaggacctggagcccctgac


 


catcttgtactatgtgggcagaacccccaaggtggagcagctgtccaacatggtggtgaagtcgtgtaagtgcagctga


 


TGFB3 Human (nucleic acid sequence):


(SEQ ID NO: 320)



Atgaagatgcacttgcaaagggctctggtggtcctggccctgctgaactttgccacggtcagcctctctctgtccacttgcaccaccttg



 


gacttcggccacatcaagaagaagagggtggaagccattaggggacagatcttgagcaagctcaggctcaccagcccccctgagcc


 


aacggtgatgacccacgtcccctatcaggtcctggccctttacaacagcacccgggagctgctggaggagatgcatggggagaggga


 


ggaaggctgcacccaggaaaacaccgagtcggaatactatgccaaagaaatccataaattcgacatgatccaggggctggcggagc


 


acaacgaactggctgtctgccctaaaggaattacctccaaggttttccgcttcaatgtgtcctcagtggagaaaaatagaaccaacctatt


 


ccgagcagaattccgggtcttgcgggtgcccaaccccagctctaagcggaatgagcagaggatcgagctcttccagatccttcggcca


 


gatgagcacattgccaaacagcgctatatcggtggcaagaatctgcccacacggggcactgccgagtggctgtcctttgatgtcactga


 


cactgtgcgtgagtggctgttgagaagagagtccaacttaggtctagaaatcagcattcactgtccatgtcacacctttcagcccaatgga


 


gatatcctggaaaacattcacgaggtgatggaaatcaaattcaaaggcgtggacaatgaggatgaccatggccgtggagatctggggc


 


gcctcaagaagcagaaggatcaccacaaccctcatctaatcctcatgatgattcccccacaccggctcgacaacccgggccaggggg


 


gtcagaggaagtactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagc


 


taaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccagg


 


aaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagatt


 


ccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacct


 


ggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgaga


 


atcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgc


 


caggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagaca


 


cagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactc


 


gctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctgga


 


gccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacga


 


gcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttc


 


tacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgg


 


gatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaact


 


cattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaaga


 


tggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatc


 


cattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctga


 


cccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatct


 


tgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttct


 


gtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaggaag


 


aagcgggctttggacaccaattactgcttccgcaacttggaggagaactgctgtgtgcgccccctctacattgacttccgacaggatctg


 


ggctggaagtgggtccatgaacctaagggctactatgccaacttctgctcaggcccttgcccatacctccgcagtgcagacacaaccca


 


cagcacggtgctgggactgtacaacactctgaaccctgaagcatctgcctcgccttgctgcgtgccccaggacctggagcccctgacc


 


atcctgtactatgttgggaggacccccaaagtggagcagctctccaacatggtggtgaagtcttgtaaatgtagctga


 


PDGFA Mouse (nucleic acid sequence):


(SEQ ID NO: 321)



Atgaggacctgggcttgcctgctgctcctcggctgcggatacctcgcccatgccctggccgaggaagccgagataccccgggagttg



 


atcgagcggctggctcgaagtcagatccacagcatccgggacctccagcgactcttggagatagactccgtaggggctgaggatgcc


 


ttggagacaagtctgagagcccatgggtcccatgccattaaccatgtgcccgagaagcggcctgtgcccattcgcaggtactgcgcca


 


ctgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattga


 


tagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgt


 


cagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtac


 


atacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgt


 


gctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtgga


 


gctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattg


 


agttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctct


 


ggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccag


 


gagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgc


 


aaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttg


 


actacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaa


 


gataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacagga


 


atgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattc


 


tggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagcca


 


tcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggt


 


atttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgca


 


ccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgc


 


cacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaa


 


gaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaacgcaggaagagaagtattgaggaagccat


 


tcctgcagtttgcaagaccaggacggtcatttacgagatacctcggagccaggtggaccccacatcggccaacttcctgatctggcccc


 


cagtgtgtggaggtgaagcgctgcactggctgttgtaacaccagcagcgtcaagtgccagccttcacgggtccaccaccgcagtgtcaa


 


ggtggccaaagtggagtatgtcaggaagaagccaaaattgaaagaggtccaggtgaggttagaggaacacctggagtgtgcatgtgc


 


gacctccaacctgaacccagaccatcgggaggaggagacagatgtgaggtga


 


PDGFA Human (nucleic acid sequence):


(SEQ ID NO: 322)



Atgaggaccttggcttgcctgctgctcctcggctgcggatacctcgcccatgttctggccgaggaagccgagatcccccgcgaggtga



 


tcgagaggctggcccgcagtcagatccacagcatccgggacctccagcgactcctggagatagactccgtagggagtgaggattcttt


 


ggacaccagcctgagagctcacggggtccatgccactaagcatgtgcccgagaagcggcccctgcccattcggaggtactgcgcca


 


ctgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattga


 


tagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgt


 


cagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtac


 


atacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgt


 


gctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtgga


 


gctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattg


 


agttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctct


 


ggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccag


 


gagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgc


 


aaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttg


 


actacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaa


 


gataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacagga


 


atgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattc


 


tggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagcca


 


tcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggt


 


atttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgca


 


ccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgc


 


cacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaa


 


gaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaacggaggaagagaagcatcgaggaagctg


 


tccccgctgtctgcaagaccaggacggtcatttacgagattcctcggagtcaggtcgaccccacgtccgccaacttcctgatctggccc


 


ccgtgcgtggaggtgaaacgctgcaccggctgctgcaacacgagcagtgtcaagtgccagccctcccgcgtccaccaccgcagcgt


 


caaggtggccaaggtggaatacgtcaggaagaagccaaaattaaaagaagtccaggtgaggttagaggagcatttggagtgcgcctg


 


cgcgaccacaagcctgaatccggattatcgggaagaggacacgggaaggcctagggagtcaggtaaaaaacggaaaagaaaaag


 


gttaaaacccacctaa


 


BDNF Mouse (nucleic acid sequence):


(SEQ ID NO: 323)



Atgttccaccaggtgagaagagtgatgaccatccttttccttactatggttatttcatacttcggttgcatgaaggcggcgcccatgaaaga



 


agtaaacgtccacggacaaggcaacttggcctacccaggtgtgcggacccatgggactctggagagcgtgaatgggcccagggcag


 


gttcgagaggtctgacgacgacatcactggctgacacttttgagcacgtcatcgaagagctgctggatgaggaccagaaggttcggcc


 


caacgaagaaaaccataaggacgcggacttgtacacttcccgggtgatgctcagcagtcaagtgcctttggagcctcctctactctttctg


 


ctggaggaatacaaaaattacctggatgccgcaaacatgtctatgagggttcggcgctactgcgccactgttcattgccaggactgtcct


 


tacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcac


 


gagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcaga


 


gtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaac


 


caagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactg


 


gaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaa


 


cccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtga


 


tcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaa


 


aatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccact


 


ctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctacta


 


ctacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcg


 


ctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtcc


 


ctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaa


 


gtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccg


 


tgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggt


 


ggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaacc


 


aggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacct


 


gaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtc


 


gaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataa


 


agcatggagacaccctagaagtaccagatgaatgcaaacggcgccactccgaccctgcccgccgtggggagctgagcgtgtgtg


 


acagtattagcgagtgggtcacagcggcagataaaaagactgcagtggacatgtctggcgggacggtcacagtcctagagaaagtcc


 


cggtatccaaaggccaactgaagcagtatttctacgagaccaagtgtaatcccatgggttacaccaaggaaggctgcaggggcataga


 


caaaaggcactggaactcgcaatgccgaactacccaatcgtatgttcgggcccttactatggatagcaaaaagagaattggctggcgat


 


tcataaggatagacacttcctgtgtatgtacactgaccattaaaaggggaagatag


 


BDNF Human (nucleic acid sequence):


(SEQ ID NO: 324)



Atgaccatccttttccttactatggttatttcatactttggttgcatgaaggctgcccccatgaaagaagcaaacatccgaggacaaggtgg



 


cttggcctacccaggtgtgcggacccatgggactctggagagcgtgaatgggcccaaggcaggttcaagaggcttgacatcattggct


 


gacactttcgaacacgtgatagaagagctgttggatgaggaccagaaagttcggcccaatgaagaaaacaataaggacgcagacttgt


 


acacgtccagggtgatgctcagtagtcaagtgcctttggagcctcctcttctctttctgctggaggaatacaaaaattacctagatgctgca


 


aacatgtccatgagggtccggcgccactctgaccctgcccgccgatactgcgccactgttcattgccaggactgtccttacgaacctg


 


atccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacat


 


actatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccg


 


caggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggc


 


gactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagacaagactggaagtggctgg


 


agacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccat


 


cggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcg


 


gaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtggagatcttaaaatgatggaag


 


atacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcc


 


tgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcc


 


tgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaac


 


tagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattccagggtccctgcaaggagatt


 


cttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtac


 


gaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtac


 


agctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaact


 


tcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgat


 


gattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgac


 


tctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgta


 


cgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgctacataaagcatggagacac


 


cctagaagtaccagatgaatgcaaacgccgagggggagctgagcgtgtgtgacagtattagtgagtgggtaacggcggcagacaaa


 


aagactgcagtggacatgtcgggcgggacggtcacagtccttgaaaaggtccctgtatcaaaaggccaactgaagcaatacttctacg


 


agaccaagtgcaatcccatgggttacacaaaagaaggctgcaggggcatagacaaaaggcattggaactcccagtgccgaactaccc


 


agtcgtacgtgcgggcccttaccatggatagcaaaagagaattggctggcgattcataaggatagacacttcttgtgtatgtacattgac


 


cattaaaaggggaagatag


 


NGF Mouse (nucleic acid sequence):


(SEQ ID NO: 325)



Atgtccatgttgttctacactctgatcactgcgtttttgatcggcgtacaggcagaaccgtacacagatagcaatgtcccagaaggagact



 


ctgtccctgaagcccactggactaaacttcagcattcccttgacacagccctccgcagagcccgcagtgcccctactgcaccaatagct


 


gcccgagtgacagggcagacccgcaacatcactgtagaccccagactgtttaagaaacggagactccactcaccccgtgtgctgttca


 


gcacccagcctccacccacctcttcagacactctggatctagacttccaggcccatggtacaatccctttcaacaggactcaccggagc


 


aagcgctactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaag


 


aaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaa


 


catgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagtt


 


ccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatgg


 


aaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatccat


 


cactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggctt


 


caacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaa


 


acaaaggaatgatctctggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattc


 


agcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgta


 


caaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgag


 


cctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacga


 


tacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtg


 


aaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgtt


 


gatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtga


 


catactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcg


 


atggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgaccccc


 


aacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatc


 


agaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgac


 


cacgcatgggagttcaagaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaagcgctcatcc


 


acccacccagtcttccacatgggggagttctcagtgtgtgacagtgtcagtgtgtgggttggagataagaccacagccacagacatcaa


 


gggcaaggaggtgacagtgctggccgaggtgaacattaacaacagtgtattcagacagtactttttgagaccaagtgccgagcctcca


 


atcctgttgagagtgggtgccggggcatcgactccaaacactggaactcatactgcaccacgactcacaccttcgtcaaggcgttgaca


 


acagatgagaagcaggctgcctggaggttcatccggatagacacagcctgtgtgtgtgtgctcagcaggaaggctacaagaagaggc


 


tga


 


NGF Human (nucleic acid sequence):


(SEQ ID NO: 326)



Atgtccatgttgttctacactctgatcacagcttttctgatcggcatacaggcggaaccacactcagagagcaatgtccctgcaggacac



 


aaccatcccccaagcccactggactaaacttcagcattcccttgacactgcccttcgcagagcccgcagcgccccggcagcggcgata


 


gctgcacgcgtggcggggcagacccgcaacattactgtggaccccaggctgtttaaaaagcggcgactccgttcaccccgtgtgctgt


 


ttagcacccagcctccccgtgaagctgcagacactcaggatctggacttcgaggtcggtggtgctgcccccttcaacaggactcacag


 


gagcaagcggtactgcgccactgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagct


 


aaagaaggagaatgtattgatagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccagga


 


aaaacatgttgccgaatgtgtcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattc


 


cagttccaggaacctggtacatacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctg


 


gatggaaccaagggggctgtgctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaat


 


cccatcactgtaaacggtggagctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgcca


 


ggcttcaacatcaccgtcattgagttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacaca


 


gcaaacaaaggaatgatctctggcctctgtggagatcttaaactgatggaagatacagacttcacttcagatccagaacaactcgc


 


tattcagcctaagatcaaccaggagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagc


 


cgtacaaggacagctgccgcaaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagc


 


gagcctcacacgtgctgcttgactacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttcta


 


cgatacatttgacaaagcaagataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttggga


 


tgtgaaggtttcacacaggaatgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactca


 


ttgttgatggaaaacagattctggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatg


 


gtgacatactgactacagccatcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatcc


 


attcgatggtaagacttgcggtatttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgac


 


ccccaacccaccgggatgcaccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatctt


 


gatcagaaatgtaacgtgtgccacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctg


 


tgaccacgcatgggagttcaagaaagaaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaagcggt


 


catcatcccatcccatcttccacaggggcgaattctcggtgtgtgacagtgtcagcgtgtgggttggggataagaccaccgccacagac


 


atcaagggcaaggaggtgatggtgttgggagaggtgaacattaacaacagtgtattcaaacagtactttttgagaccaagtgccggga


 


cccaaatcccgttgacagcgggtgccggggcattgactcaaagcactggaactcatattgtaccacgactcacacctttgtcaaggcgc


 


tgaccatggatggcaagcaggctgcctggcggtttatccggatagatacggcctgtgtgtgtgtgctcagcaggaaggctgtgagaag


 


agcctga


 


Albumin Mouse (nucleic acid sequence):


(SEQ ID NO: 327)



Atgaagtgggtaacctttctcctcctcctcttcgtctccggctctgctttttccaggggtgtgtttcgccgatactgcgccactgttcattgcc



 


aggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgt


 


ggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaa


 


ttgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgg


 


gtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaa


 


gacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccct


 


atcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttca


 


aactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctg


 


tggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttga


 


cggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccat


 


caacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagg


 


gagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaa


 


ttccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactc


 


ttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggag


 


gagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctg


 


aagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtatttgcggt


 


aactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccgaaga


 


acagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagc


 


ctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaaga


 


atgctacataaagcatggagacaccctagacgtaccagatgaatgcaaacgccgagaagcacacaagagtgagatcgcccatcg


 


gtataatgatttgggagaacaacatttcaaaggcctagtcctgattgccttttcccagtatctccagaaatgctcatacgatgagcatgcca


 


aattagtgcaggaagtaacagactttgcaaagacgtgtgttgccgatgagtctgccgccaactgtgacaaatcccttcacactctttttgga


 


gataagttgtgtgccattccaaacctccgtgaaaactatggtgaactggctgactgctgtacaaaacaagagcccgaaagaaacgaatg


 


tttcctgcaacacaaagatgacaaccccagcctgccaccatttgaaaggccagaggctgaggccatgtgcacctcctttaaggaaaacc


 


caaccacctttatgggacactatttgcatgaagttgccagaagacatccttatttctatgccccagaacttctttactatgctgagcagtacaa


 


tgagattctgacccagtgttgtgcagaggctgacaaggaaagctgcctgaccccgaagcttgatggtgtgaaggagaaagcattggtct


 


catctgtccgtcagagaatgaagtgctccagtatgcagaagtttggagagagagcttttaaagcatgggcagtagctcgtctgagccag


 


acattccccaatgctgactttgcagaaatcaccaaattggcaacagacctgaccaaagtcaacaaggagtgctgccatggtgacctgct


 


ggaatgcgcagatgacagggcggaacttgccaagtacatgtgtgaaaaccaggcgactatctccagcaaactgcagacttgctgcgat


 


aaaccactgttgaagaaagcccactgtcttagtgaggtggagcatgacaccatgcctgctgatctgcctgccattgctgctgattttgttga


 


ggaccaggaagtgtgcaagaactatgctgaggccaaggatgtcttcctgggcacgttcttgtatgaatattcaagaagacaccctgatta


 


ctctgtatccctgttgctgagacttgctaagaaatatgaagccactctggaaaagtgctgcgctgaagccaatcctcccgcatgctacggc


 


acagtgcttgctgaatttcagcctcttgtagaagagcctaagaacttggtcaaaaccaactgtgatctttacgagaagcttggagaatatgg


 


attccaaaatgccattctagttcgctacacccagaaagcacctcaggtgtcaaccccaactctcgtggaggctgcaagaaacctaggaa


 


gagtgggcaccaagtgttgtacacttcctgaagatcagagactgccttgtgtggaagactatctgtctgcaatcctgaaccgtgtgtgtct


 


gctgcatgagaagaccccagtgagtgagcatgttaccaagtgctgtagtggatccctggtggaaaggcggccatgcttctctgctctga


 


cagttgatgaaacatatgtccccaaagagtttaaagctgagaccttcaccttccactctgatatctgcacacttccagagaaggagaagca


 


gattaagaaacaaacggctcttgctgagctggtgaagcacaagcccaaggctacagcggagcaactgaagactgtcatggatgacttt


 


gcacagttcctggatacatgttgcaaggctgctgacaaggacacctgcttctcgactgagggtccaaaccttgtcactagatgcaaagac


 


gccttagcctaa


 


Albumin Human (nucleic acid sequence):


(SEQ ID NO: 328)



atgaagtgggtaacctttatttcccttctttttctctttagctcggcttattccagggggtgtgtttcgtcgatactgcgccactgttcattgccag



 


gactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgatagcagctgtggc


 


acctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtcagtatgtaattga


 


atgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacatacgtgttgggtc


 


aaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgctgaccaagac


 


aagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtggagctgaccctatca


 


tcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagttcttcaaact


 


gatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctggcctctgtgga


 


gatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccaggagtttgacggt


 


tgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaaccccatcaac


 


ttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgactacagggag


 


acgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagataccaattcc


 


agggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgttgactcttac


 


actgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggttggaggag


 


aagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcctacctgaag


 


ctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatcfcattcgatggtaagacttgcggtatttgcggtaact


 


acaaccaggatttcagtgatgattcttttgatgctgaaaggagcctgtgatctgacccccaacccaccgggatgcaccgaagaacag


 


aaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccacaagcctgac


 


cgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaagaaagaatgcta


 


cataaagcatggagacaccctagaagtaccagatgaatgcaaacgtcgagatgcacacaagagtgaggttgctcatcggtttaaag


 


atttgggagaagaaaatttcaaagccttggtgttgattgcctttgctcagtatcttcagcagtgtccatttgaagatcatgtaaaattagtgaat


 


gaagtaactgaatttgcaaaaacatgtgttgctgatgagtcagctgaaaattgtgacaaatcacttcataccctttttggagacaaattatgc


 


acagttgcaactcttcgtgaaacctatggtgaaatggctgactgctgtgcaaaacaagaacctgagagaaatgaatgcttcttgcaacac


 


aaagatgacaacccaaacctcccccgattggtgagaccagaggttgatgtgatgtgcactgcttttcatgacaatgaagagacatttttga


 


aaaaatacttatatgaaattgccagaagacatccttacttttatgccccggaactccttttctttgctaaaaggtataaagctgcttttacagaa


 


tgttgccaagctgctgataaagctgcctgcctgttgccaaagctcgatgaacttcgggatgaagggaaggcttcgtctgccaaacagag


 


actcaagtgtgccagtctccaaaaatttggagaaagagctttcaaagcatgggcagtagctcgcctgagccagagatttcccaaagctg


 


agtttgcagaagtttccaagttagtgacagatcttaccaaagtccacacggaatgctgccatggagatctgcttgaatgtgctgatgacag


 


ggcggaccttgccaagtatatctgtgaaaatcaagattcgatctccagtaaactgaaggaatgctgtgaaaaacctctgttggaaaaatcc


 


cactgcattgccgaagtggaaaatgatgagatgcctgctgacttgccttcattagctgctgattttgttgaaagtaaggatgtttgcaaaaac


 


tatgctgaggcaaaggatgtcttcctgggcatgtttttgtatgaatatgcaagaaggcatcctgattactctgtcgtgctgctgctgagactt


 


gccaagacatatgaaaccactctagagaagtgctgtgccgctgcagatcctcatgaatgctatgccaaagtgttcgatgaatttaaacctc


 


ttgtggaagagcctcagaatttaatcaaacaaaattgtgagctttttgagcagcttggagagtacaaattccagaatgcgctattagttcgtt


 


acaccaagaaagtaccccaagtgtcaactccaactcttgtagaggtctcaagaaacctaggaaaagtgggcagcaaatgttgtaaacat


 


cctgaagcaaaaagatgccctgtgcagaagactatctatccgtggtcctgaaccagttatgtgtgttgcatgagaaaacgccagtaagt


 


gacagagtcaccaaatgctgcacagaatccttggtgaacaggcgaccatgctttcagctctggaagtcgatgaaacatacgttcccaaa


 


gagtttaatgctgaaacattcaccttccatgcagatatatgcacactttctgagaaggagagacaaatcaagaaacaaactgcacttgttg


 


agctcgtgaaacacaagcccaaggcaacaaaagagcaactgaaagctgttatggatgatttcgcagcttttgtagagaagtgctgcaag


 


gctgacgataaggagacctgctttgccgaggagggtaaaaaacttgttgctgcaagtcaagctgccttaggcttataa


 


Calcitonin Mouse (nucleic acid sequence):


(SEQ ID NO: 329)



Atgggcttcctgaagttctcccctttcctggttgtcagcatcttgctcctgtaccaggcatgcagcctccaggcagtgcctttgaggtcaat



 


cttggaaagcagcccaggcatggccactctcagtgaagaagaagttcgcctgctggctgcactggtgcaggactatatgcagatgaaa


 


gccagggagctggagcaggaggaagagcaggaggctgagggctctagcttgagacagccccagatctaagcggtactgcgccactg


 


ttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattgata


 


gcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtgtca


 


gtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtacata


 


cgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgtgct


 


gaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgaggaatcccatcactgtaaacggtggagct


 


gaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattgagt


 


tcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctctgg


 


cctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccagga


 


gtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgcaac


 


cccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttgact


 


acagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaagat


 


accaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacaggaatgt


 


tgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattctggt


 


tggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagccatcct


 


acctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggtattt


 


gcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgcaccg


 


aagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgccac


 


aagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaaga


 


aagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaagcggtgtgggaatctgagtacctgcatgct


 


gggcacgtacacacaagacctcaacaagtttcacaccttcccccaaacttcaattggggttgaagcacctggcaagaaaagggatgtg


 


gccaaggacttggagacaaaccaccaatcccattttggcaactaa


 


Calcitonin Human (nucleic acid sequence):


(SEQ ID NO: 330)



Atgggcttccaaaagttctcccccttcctggctctcagcatcttggtcctgttgcaggcaggcagcctccatgcagcaccattcaggtctg



 


ccctggagagcagcccagcagacccggccacgctcagtgaggacgaagcgcgcctcctgctggctgcactggtgcaggactatgtg


 


cagatgaaggccagtgagctggagcaggagcaagagagagagggctccagcctggacagccccagatctaagcggtactgcgcc


 


actgttcattgccaggactgtccttacgaacctgatccaccaaacacagttccaacttcctgtgaagctaaagaaggagaatgtattg


 


atagcagctgtggcacctgcacgagagacatactatcagatggactgtgtgaaaataaaccaggaaaaacatgttgccgaatgtg


 


tcagtatgtaattgaatgcagagtagaggccgcaggatggtttagaacattctatggaaagagattccagttccaggaacctggtac


 


atacgtgttgggtcaaggaaccaagggcggcgactggaaggtgtccatcaccctggagaacctggatggaaccaagggggctgt


 


gctgaccaagacaagactggaagtggctggagacatcattgacatcgctcaagctactgagaatcccatcactgtaaacggtgga


 


gctgaccctatcatcgccaacccgtacaccatcggcgaggtcaccatcgctgttgttgagatgccaggcttcaacatcaccgtcattg


 


agttcttcaaactgatcgtgatcgacatcctcggaggaagatctgtaagaatcgccccagacacagcaaacaaaggaatgatctct


 


ggcctctgtggagatcttaaaatgatggaagatacagacttcacttcagatccagaacaactcgctattcagcctaagatcaaccag


 


gagtttgacggttgtccactctatggaaatcctgatgacgttgcatactgcaaaggtcttctggagccgtacaaggacagctgccgc


 


aaccccatcaacttctactactacaccatctcctgcgccttcgcccgctgtatgggtggagacgagcgagcctcacacgtgctgcttg


 


actacagggagacgtgcgctgctcccgaaactagaggaacctgcgttttgtctggacatactttctacgatacatttgacaaagcaa


 


gataccaattccagggtccctgcaaggagattcttatggccgccgactgtttctggaacacttgggatgtgaaggtttcacacagga


 


atgttgactcttacactgaagtagagaaagtacgaatcaggaaacaatcgactgtagtagaactcattgttgatggaaaacagattc


 


tggttggaggagaagccgtgtccgtcccgtacagctctcagaacacttccatctactggcaagatggtgacatactgactacagcca


 


tcctacctgaagctctggtggtcaagttcaacttcaagcaactgctcgtcgtacatattagagatccattcgatggtaagacttgcggt


 


atttgcggtaactacaaccaggatttcagtgatgattcttttgatgctgaaggagcctgtgatctgacccccaacccaccgggatgca


 


ccgaagaacagaaacctgaagctgaacgactctgcaatagtctcttcgccggtcaaagtgatcttgatcagaaatgtaacgtgtgc


 


cacaagcctgaccgtgtcgaacgatgcatgtacgagtattgcctgaggggacaacagggtttctgtgaccacgcatgggagttcaa


 


gaaagaatgctacataaagcatggagacaccctagaagtaccagatgaatgcaaaaagcggtgcggtaatctgagtacttgcatg


 


ctgggcacatacacgcaggacttcaacaagtttcacacgttcccccaaactgcaattggggttggagcacctggaaagaaaagggatat


 


gtccagcgacttggagagagaccatcgccctcatgttagcatgccccagaatgccaactaa






Due to the redundancy of the genetic code, any fusion protein of the present invention could be specified by any number of nucleic acid sequences in which synonymous base changes have been incorporated. Therefore, the nucleic acid sequences listed should be taken as case examples of one such instance for each propeptide-luciferase fusion protein, rather than the only tolerated nucleic acid sequence. The amino acid sequence of each construct ultimately determines the function of the fusion protein, though many possible nucleic acid sequences can specify each the sequence of each peptide.
The nucleic acid sequences of SEQ ID NOs: 139-330 can be introduced into cells for expression. After transcription of these sequences into RNA and subsequent translation into a peptide, the peptide is processed. For example, for those propeptides of the present invention in which the mature peptide is secreted, cleavage will occur at the cleavage sites by the appropriate protease, thereby freeing the bioluminescent protein from the mature peptide. Then, both the bioluminescent protein and the mature peptide are secreted from the cell simultaneously. In other embodiments, for those propeptides of the present invention in which the mature peptide is a transmembrane protein expressed at the cell surface, the cleavage occurs at the cleavage sites before or after the mature peptide is expressed on the cell surface. The sequences outlined in SEQ ID NO: 139 through SEQ ID NO: 330 encode an amino acid sequence, or a propeptide-luciferase fusion protein. Exemplary fusion proteins encoded by SEQ ID NO: 139-204 are listed below by example, and are not to be construed as limiting the present invention to the fusion peptides disclosed below. One of ordinary skill in the art would readily be able to determine the amino acid sequences of any of SEQ ID NO: 139-330.








Proamylin-luciferase Mouse (amino acid sequence)



(SEQ ID NO: 331)



MMCISKLPAVLLILSVALNHLRATPVRSGSNPQMDKRKCNTATCATQRLANFLVRSSNN



 


LGPVLPPTNVGSNTYGKRNAKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEV


 


LKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIP


 


EIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQ


 


VDKIKGAGGDKRNAAGDPNRESLDFLLV


 


Proamylin-luciferase Human (amino acid sequence)


(SEQ ID NO: 332)



MGILKLQVFLIVLSVALNHLKATPIESHQVEKRKCNTATCATQRLANFLVHSSNNFGAIL



 


SSTNVGSNTYGKRNAKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEME


 


ANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFK


 


DLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKG


 


AGGDKRNAVEVLKREPLNYLPL


 


Proinsulin-luciferase Mouse (amino acid sequence)


(SEQ ID NO: 333)



MALWMRFLPLLALLFLWESHPTQAFVKQHLCGSHLVEALYLVCGERGFFYTPMSRREV



 


EDPQVAQLELGGGPGAGDLQTLALEVAQQKRKPTENNEDFNIVAVASNFATTDLDADR


 


GKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKES


 


AQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ


 


RCATFASKIQGQVDKIKGAGGDKRGIVDQCCTSICSLYQLENYCN


 


Proinsulin-luciferase Human (amino acid sequence)


(SEQ ID NO: 334)



MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREA



 


EDLQVGQVELGGGPGAGSLQPLALEGSLQKRKPTENNEDFNIVAVASNFATTDLDADRG


 


KLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA


 


QGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQR


 


CATFASKIQGQVDKIKGAGGDKRGIVEQCCTSICSLYQLENYCN


 


Proglucagon (includes GRPP, glucagon, GLP-1, GLP-2) Mouse (amino


acid sequence)


(SEQ ID NO: 335)



MKTIYFVAGLLIMLVQGSWQHALQDTEENPRSFPASQTEAHEDPDEMNEDKRHSQGTFT



 


SDYSKYLDSRRAQDFVQWLMNTKRNRNNIAKRHDEFERHAEGTFTSDVSSYLEGQAAK


 


EFIAWLVKGRGRRDFPEEVAIAEELGRKRRKPTENNEDFNIVAVASNFATTDLDADRGK


 


LPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQ


 


GGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRC


 


ATFASKIQGQVDKIKGAGGDKRGRRHADGSFSDEMSTILDNLATRDFINWLIQTKITDKK


 


Proglucagon (includes GRPP, glucagon, GLP-1, GLP-2) Human (amino


acid sequence)


(SEQ ID NO: 336)



MKSIYFVAGLFVMLVQGSWQRSLQDTEEKSRSFSASQADPLSDPDQMNEDKRHSQGTFT



 


SDYSKYLDSRRAQDFVQWLMNTKRNRNNIAKRHDEFERHAEGTFTSDVSSYLEGQAAK


 


EFIAWLVKGRGRRDFPEEVAIVEELGRKRRKPTENNEDFNIVAVASNFATTDLDADRGK


 


LPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQ


 


GGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRC


 


ATFASKIQGQVDKIKGAGGDKRGRRHADGSFSDEMNTILDNLAARDFINWLIQTKITDR


 


K


 


Peptide YY Mouse (amino acid sequence)


(SEQ ID NO: 337)



MVAVRRPWPVTVAMLLILLACLGALVDAYPAKPEAPGEDASPEELSRYYASLRHYLNL



 


VTRQRYGKRKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARK


 


AGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPME


 


QFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDK


 


RRDVPAALFSKLLFTDDSDSENLPFRPEGLDQW


 


Peptide YY Human (amino acid sequence)


(SEQ ID NO: 338)



MVFVRRPWPALTTVLLALLVCLGALVDAYPIKPEAPGEDASPEELNRYYASLRHYLNLV



 


TRQRYGKRKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKA


 


GCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQ


 


FIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDKR


 


RDGPDTLLSKTFFPDGEDRPVRSRSEGPDLW


 


Neuropeptide Y Mouse (amino acid sequence)


(SEQ ID NO: 339)



MLGNKRMGLCGLTLALSLLVCLGILAEGYPSKPDNPGEDAPAEDMARYYSALRHYINLI



 


TRQRYGKRKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKA


 


GCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQ


 


FIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDKR


 


RSSPETLISDLLMKESTENAPRTRLEDPSMW


 


Neuropeptide Y Human (amino acid sequence)


(SEQ ID NO: 340)



MLGNKRLGLSGLTLALSLLVCLGALAEAYPSKPDNPGEDAPAEDMARYYSALRHYINLI



 


TRQRYGKRKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKA


 


GCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQ


 


FIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDKR


 


RSSPETLISDLLMRESTENVPRTRLEDPAMW


 


Pancreatic polypeptide Mouse (amino acid sequence)


(SEQ ID NO: 341)



MAVAYCCLSLFLVSTWVALLLQPLQGTWGAPLEPMYPGDYATPEQMAQYETQLRRYIN



 


TLTRPRYGKRKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARK


 


AGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPME


 


QFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDK


 


RRAEEENTGGLPGVQLSPCTSPPVGLIPCSAPWS


 


Pancreatic polypeptide Human (amino acid sequence)


(SEQ ID NO: 342)



MAAARLCLSLLLLSTCVALLLQPLLGAQGAPLEPVYPGDNATPEQMAQYAADLRRYIN



 


MLTRPRYGKRKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANAR


 


KAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEP


 


MEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGG


 


DKRRHKEDTLAFSEWGSPHAAVPRELSPLDL


 


Somatostatin Mouse (amino acid sequence)


(SEQ ID NO: 343)



MLSCRLQCALAALCIVLALGGVTGAPSDPRLRQFLQKSLAAATGKQELAKYFLAELRKK



 


PTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICL


 


SHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCV


 


DCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDRKLSEPNQTEN


 


DALEPEDLPQAAEQDEMRLELQRSANSNPAMAPRERKAGCKNFFWKTFTSC


 


Somatostatin Human (amino acid sequence)


(SEQ ID NO: 345)



MLSCRLQCALAALSIVLALGCVTGAPSDPRLRQFLQKSLAAAAGKQELAKYFLAELRKK



 


PTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICL


 


SHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCV


 


DCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDRKLSEPNQTEN


 


DALEPEDLSQAAEQDEMRLELQRSANSNPAMAPRERKAGCKNFFWKTFTSC


 


GHRH Mouse (amino acid sequence)


(SEQ ID NO: 346)



MLLWVLFVILILTSGSHCSLPPSPPFRMQRHVDAIFTTNYRKLLSQLYARKVIQDIMNKQ



 


GERIQEQRARLSRQEDSMWTEDKQMTLESIRRKPTENNEDFNIVAVASNFATTDLDADR


 


GKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKES


 


AQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ


 


RCATFASKIQGQVDKIKGAGGDRRLQGFPRMKPSADA


 


GHRH Human (amino acid sequence)


(SEQ ID NO: 347)



MPLWVFFFVILTLSNSSHCSPPPPLTLRMRRYADAIFTNSYRKVLGQLSARKLLQDIMSR



 


QQGESNQERGARARLGRQVDSMWAEQKQMELESILVALRRKPTENNEDFNIVAVASNF


 


ATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRC


 


HTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQPIAQVDLCVDCTTGCLKGLANVQCS


 


DLLKKWLPQRCATFASKIQGQVDKIKGAGGDRRLQKHRNSQG


 


POMC (ACTH, MSH) Mouse (amino acid sequence)


(SEQ ID NO: 348)



MPRFCYSRSGALLLALLLQTSIDVWSWCLESSQCQDLTTESNLLACIRACKLDLSLETPV



 


FPGNGDEQPLTENPRKYVMGHFRWDRFGPRNSSSAGSAAQRRAEEEAVWGDGSPEPSP


 


REGKRKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCT


 


RGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIA


 


QVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDKRSYS


 


MEHFRWGKPVGKKRRPVKVYPNVAENESAEAFPLEFKRELEGERPLGLEQVLESDAEK


 


DDGPYRVEHFRWSNPPKDKRYGGFMTSEKSQTPLVTLFKNAIIKNAHKKGQ


 


POMC (ACTH, MSH) Human (amino acid sequence)


(SEQ ID NO: 349)



MPRSCCSRSGALLLALLLQASMEVRGWCLESSQCQDLTTESNLLECIRACKPDLSAETPM



 


FPGNGDEQPLTENPRKYVMGHFRWDRFGRRNSSSSGSSGAGQKREDVSAGEDCGPLPE


 


GGPEPRSDGAKPGPREGKRKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVL


 


KEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEI


 


PGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQV


 


DKIKGAGGDKRSYSMEHFRWGKPVGKKRRPVKVYPNGAEDESAEAFPLEFKRELTGQR


 


LREGDGPDGPADDGAGAQADLEHSLLVAAEKKDEGPYRMEHFRWGSPPKDKRYGGFM


 


TSEKSQTPLVTLFKNAIIKNAYKKGE


 


Oxytocin Mouse (amino acid sequence)


(SEQ ID NO: 350)



MACPSLACCLLGLLALTSACYIQNCPLGGKRKPTENNEDFNIVAVASNFATTDLDADRG



 


KLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA


 


QGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQR


 


CATFASKIQGQVDKIKGAGGDKRAVLDLDMRKCLPCGPGGKGRCFGPSICCADELGCFV


 


GTAEALRCQEENYLPSPCQSGQKPCGSGGRCAATGICCSPDGCRTDPACDPESAFSER


 


Oxytocin Human (amino acid sequence)


(SEQ ID NO: 351)



MAGPSLACCLLGLLALTSACYIQNCPLGGKRKPTENNEDFNIVAVASNFATTDLDADRG



 


KLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA


 


QGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQR


 


CATFASKIQGQVDKIKGAGGDKRAAPDLDVRKCLPCGPGGKGRCFGPNICCAEELGCFV


 


GTAEALRCQEENYLPSPCQSGQKACGSGGRCAVLGLCCSPDGCHADPACDAEATFSQR


 


Vasopressin-Neurophysin-2 Mouse (amino acid sequence)


(SEQ ID NO: 352)



MLARMLNTTLSACFLSLLAFSSACYFQNCPRGGKRKPTENNEDFNIVAVASNFATTDLD



 


ADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGD


 


KESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKW


 


LPQRCATFASKIQGQVDKIKGAGGDKRAISDMELRQCLPCGPGGKGRCFGPSICCADELG


 


CFVGTAEALRCQEENYLPSPCQSGQKPCGSGGRCAAVGICCSDESCVAEPECHDGFFRLT


 


RAREPSNATQLDGPARALLLRLVQLAGTRESVDSAKPRVY


 


Vasopressin-Neurophysin-2 Human (amino acid sequence)


(SEQ ID NO: 353)



MPDTMLPACFLGLLAFSSACYFQNCPRGGKRKPTENNEDFNIVAVASNFATTDLDADRG



 


KLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA


 


QGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQR


 


CATFASKIQGQVDKIKGAGGDKRAMSDLELRQCLPCGPGGKGRCFGPSICCADELGCFV


 


GTAEALRCQEENYLPSPCQSGQKACGSGGRCAAFGVCCNDESCVTEPECREGFHRRARA


 


SDRSNATQLDGPAGALLLRLVQLAGAPEPFEPAQPDAY


 


Gonadotropin-releasing hormone (GnRH) Mouse (amino acid sequence)


(SEQ ID NO: 354)



MILKLMAGILLLTVCLEGCSSQHWSYGLRPGGKRKPTENNEDFNIVAVASNFATTDLDA



 


DRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDK


 


ESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWL


 


PQRCATFASKIQGQVDKIKGAGGDKRNTEHLVESFQEMGKEVDQMAEPQHFECTVHWP


 


RSPLRDLRGALESLIEEEARQKKM


 


Gonadotropin-releasing hormone (GnRH) Human (amino acid sequence)


(SEQ ID NO: 355)



MKPIQKLLAGLILLTWCVEGCSSQHWSYGLRPGGKRKPTENNEDFNIVAVASNFATTDL



 


DADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEG


 


DKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKK


 


WLPQRCATFASKIQGQVDKIKGAGGDKRDAENLIDSFQEIVKEVGQLAETQRFECTTHQ


 


PRSPLRDLKGALESLIEEETGQKKI


 


Thyroid-stimulating hormone, beta subunit (TSHB) Mouse (amino


acid sequence)


(SEQ ID NO: 356)



MSAAVLLSVLFALACGQAASFCIPTEYTMYVDRRECAYCLTINTTICAGYCMTRDINGK



 


LFLPKYALSQDVCTYRDFIYRTVEIPGCPHHVTPYFSFPVAISCKCGKCNTDNSDCIHEAV


 


RTNYCTKPQSFYLKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEAN


 


ARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDL


 


EPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGA


 


GGDYLGGFSV


 


Thyroid-stimulating hormone, beta subunit (TSHB) Human (amino


acid sequence)


(SEQ ID NO: 334)



MTALFLMSMLFGLTCGQAMSFCIPTEYTMHIERRECAYCLTINTTICAGYCMTRDINGKL



 


FLPKYALSQDVCTYRDFIYRTVEIPGCPLHVAPYFSYPVALSCKCGKCNTDYSDCIHEAIK


 


TNYCTKPQKSYLKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANA


 


RKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEP


 


MEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGG


 


DYLVGFSV


 


Cortisol-releasing factor (CRF) Mouse (amino acid sequence)


(SEQ ID NO: 357)



MRLRLLVSAGMLLVALSSCLPCRALLSRGSVPRAPRAPQPLNFLQPEQPQQPQPVLIRMG



 


EEYFLRLGNLNRSPAARLSPNSTPLTAGRGSRPSHDQAAANFFRVLLQQLQMPQRSLDSR


 


AEPAERGAEDALGGHQGALERERRKPTENNEDFNIVAVASNFATTDLDADRGKLPGKK


 


LPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEA


 


IVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASK


 


IQGQVDKIKGAGGDRRSEEPPISLDLTFHLLREVLEMARAEQLAQQAHSNRKLMEIIGK


 


Cortisol-releasing factor (CRF) Human (amino acid sequence)


(SEQ ID NO: 358)



MRLPLLVSAGVLLVALLPCPPCRALLSRGPVPGARQAPQHPQPLDFFQPPPQ



 


SEQPQQPQARPVLLRMGEEYFLRLGNLNKSPAAPLSPASSLLAGGSGSRPSPEQATANFF


 


RVLLQQLLLPRRSLDSPAALAERGARNALGGHQEAPERERRKPTENNEDFNIVAVASNF


 


ATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRC


 


HTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQPIAQVDLCVDCTTGCLKGLANVQCS


 


DLLKKWLPQRCATFASKIQGQVDKIKGAGGDRRSEEPPISLDLTFHLLREVLEMARAEQL


 


AQQAHSNRKLMEIIGK


 


Atrial natriuretic peptide (ANP) Mouse (amino acid sequence)


(SEQ ID NO: 359)



MGSFSITLGFFLVLAFWLPGHIGANPVYSAV SNTDLMDFKNLLDHLEEKMPVEDEVMPP



 


QALSEQTEEAGAALSSLPEVPPWTGEVNPPLRDGSALGRSPWDPSDRSALLKSKLRALLA


 


GPRSKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTR


 


GCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQ


 


VDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDRSLRRS


 


SCFGGRIDRIGAQSGLGCNSFRYRR


 


Atrial natriuretic peptide (ANP) Human (amino acid sequence)


(SEQ ID NO: 360)



MSSFSTTTVSFLLLLAFQLLGQTRANPMYNAVSNADLMDFKNLLDHLEEKMPLEDEVVP



 


PQVLSEPNEEAGAALSPLPEVPPWTGEVSPAQRDGGALGRGPWDSSDRSALLKSKLRAL


 


LTAPRSKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCT


 


RGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIA


 


QVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDRSLRR


 


SSCFGGRMDRIGAQSGLGCNSFRY


 


Brain natriuretic peptide (BNP) Human (amino acid sequence)


(SEQ ID NO: 361)



MDPQTAPSRALLLLLFLHLAFLGGRSHPLGSPGSASDLETSGLQEQRNHLQGKLSELQVE



 


QTSLEPLQESPRPTGVWKSREVATEGIRGHRKMVLYTLRAPRSKPTENNEDFNIVAVASN


 


FATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGR


 


CHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQC


 


SDLLKKWLPQRCATFASKIQGQVDKIKGAGGDRSRSPKMVQGSGCFGRKMDRISSSSGL


 


GCKVLRRH


 


Renin Mouse (amino acid sequence)


(SEQ ID NO: 362)



MDRRRMPLWALLLLWSPCTFSLPTRTATFERIPLKKMPSVREILEERGVDMTRLSAEWG



 


VFTKRKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCT


 


RGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIA


 


QVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDKRPSL


 


TNLTSPVVLTNYLNTQYYGEIGIGTPPQTFKVIFDTGSANLWVPSTKCSRLYLACGIHSLY


 


ESSDSSSYMENGSDFTIHYGSGRVKGFLSQDSVTVGGITVTQTFGEVTELPLIPFMLAKFD


 


GVLGMGFPAQAVGGVTPVFDHILSQGVLKEEVFSVYYNRGSHLLGGEVVLGGSDPQHY


 


QGNFHYVSISKTDSWQITMKGVSVGSSTLLCEEGCAVVVDTGSSFISAPTSSLKLIMQAL


 


GAKEKRIEEYVVNCSQVPTLPDISFDLGGRAYTLSSTDYVLQYPNRRDKLCTLALHAMDI


 


PPPTGPVWVLGATFIRKFYTEFDRHNNRIGFALAR


 


Renin Human (amino acid sequence)


(SEQ ID NO: 363)



MDGWRRMPRWGLLLLLWGSCTFGLPTDTTTFKRKPTENNEDFNIVAVASNFATTDLDA



 


DRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDK


 


ESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWL


 


PQRCATFASKIQGQVDKIKGAGGDKRIFLKRMPSIRESLKERGVDMARLGPEWSQPMKR


 


LTLGNTTSSVILTNYMDTQYYGEIGIGTPPQTFKVVFDTGSSNVWVPSSKCSRLYTACVY


 


HKLFDASDSSSYKHNGTELTLRYSTGTVSGFLSQDIITVGGITVTQMFGEVTEMPALPFM


 


LAEFDGVVGMGFIEQAIGRVTPIFDNIISQGVLKEDVFSFYYNRDSENSQSLGGQIVLGGS


 


DPQHYEGNFHYINLIKTGVWQIQMKGVSVGSSTLLCEDGCLALVDTGASYISGSTSSIEK


 


LMEALGAKKRLFDYVVKCNEGPTLPDISFHLGGKEYTLTSADYVFQESYSSKKLCTLAIH


 


AMDIPPPTGPTWALGATFIRKFYTEFDRRNNRIGFALAR


 


Galanin Mouse (amino acid sequence)


(SEQ ID NO: 364)



MARGSVILLGWLLLVVTLSATLGLGMPAKEKRGKPTENNEDFNIVAVASNFATTDLDA



 


DRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDK


 


ESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWL


 


PQRCATFASKIQGQVDKIKGAGGDKRGWTLNSAGYLLGPHAIDNHRSFSDKHGLTGKRE


 


LQLEVEERRPGSVDVPLPESNIVRTIMEFLSFLHLKEAGALDSLPGIPLATSSEDLEKS


 


Galanin Human (amino acid sequence)


(SEQ ID NO: 365)



MARGSALLLASLLLAAALSASAGLWSPAKEKRGKPTENNEDFNIVAVASNFATTDLDAD



 


RGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKE


 


SAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLP


 


QRCATFASKIQGQVDKIKGAGGDKRGWTLNSAGYLLGPHAVGNHRSFSDKNGLTSKRE


 


LRPEDDMKPGSFDRSIPENNIMRTIIEFLSFLHLKEAGALDRLLDLPAAASSEDIERS


 


Orexin Mouse (amino acid sequence)


(SEQ ID NO: 366)



MNFPSTKVPWAAVTLLLLLLLPPALLSLGVDAQPLPDCCRQKTCSCRLYELLHGAGNHA



 


AGILTLGKRRPGPPGLQGRLQRLLQANGNHAAGILTMGRRKPTENNEDFNIVAVASNFA


 


TTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCH


 


TYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSD


 


LLKKWLPQRCATFASKIQGQVDKIKGAGGDGRRAGAELEPHPCSGRGCPTVTTTALAPR


 


GGSGV


 


Orexin Human (amino acid sequence)


(SEQ ID NO: 367)



MNLPSTKVSWAAVTLLLLLLLLPPALLSSGAAAQPLPDCCRQKTCSCRLYELLHGAGNH



 


AAGILTLGKRRSGPPGLQGRLQRLLQASGNHAAGILTMGRRKPTENNEDFNIVAVASNF


 


ATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRC


 


HTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQPIAQVDLCVDCTTGCLKGLANVQCS


 


DLLKKWLPQRCATFASKIQGQVDKIKGAGGDGRRAGAEPAPRPCLGRRCSAPAAASVA


 


PGGQSGI


 


Ghrelin-Obestatin Mouse (amino acid sequence)


(SEQ ID NO: 368)



MLSSGTICSLLLLSMLWMDMAMAGSSFLSPEHQKAQQRKESKKPPAKLQPRAKPTENN



 


EDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKC


 


TPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTG


 


CLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDPRALEGWLHPEDRGQA


 


EETEEELEIRFNAPFDVGIKLSGAQYQQHGRALGKFLQDILWEEVKEAPADK


 


Ghrelin-Obestatin Human (amino acid sequence)


(SEQ ID NO: 369)



MPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQRKESKKPPAKLQPRAKPTENNED



 


FNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTP


 


KMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQHAQVDLCVDCTTGCL


 


KGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDPRALAGWLRPEDGGQAE


 


GAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK


 


Cholecystokinin Mouse (amino acid sequence)


(SEQ ID NO: 370)



MKSGVCLCVVMAVLAAGALAQPVVPAEATDPVEQRAQEAPRRQLRAKPTENNEDFNIV



 


AVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMK


 


KFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGL


 


ANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDRRQLRAVLRTDGEPRARLGAL


 


LARYIQQVRKAPSGRMSVLKNLQSLDPSHRISDRDYMGWMDFGRRSAEDYEYPS


 


Cholecystokinin Human (amino acid sequence)


(SEQ ID NO: 371)



MNSGVCLCVLMAVLAAGALTQPVPPADPAGSGLQRAEEAPRRQLRVKPTENNEDFNIV



 


AVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMK


 


KFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGL


 


ANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDRRQLRVSQRTDGESRAHLGAL


 


LARYIQQARKAPSGRMSIVKNLQNLDPSHRISDRDYMGWMDFGRRSAEEYEYPS


 


Gastrin Mouse (amino acid sequence)


(SEQ ID NO: 372)



MPRLCVYMLVLVLALATFSEASWKPRSQLQDASSGPGTNEDLEQRQFNKLGSASHHRR



 


QLGPQGPQHFIADLSKKQRPRMEEEEEAYGWMDFGRRSKPTENNEDFNIVAVASNFATT


 


DLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTY


 


EGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQPIAQVDLCVDCTTGCLKGLANVQCSDLL


 


KKWLPQRCATFASKIQGQVDKIKGAGGDRRSAEEDQ


 


Gastrin Human (amino acid sequence)


(SEQ ID NO: 373)



MQRLCVYVLIFALALAAFSEASWKPRSQQPDAPLGTGANRDLELPWLEQQGPASHHRR



 


QLGPQGPPHLVADPSKKQGPWLEEEEEAYGWMDFGRRSKPTENNEDFNIVAVASNFAT


 


TDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHT


 


YEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDL


 


LKKWLPQRCATFASKIQGQVDKIKGAGGDRRSAEDEN


 


Protachykinin-1 (Substance P, Neurokinin A, Neuropeptide K,


Neuropeptide gamma) Mouse (amino acid sequence)


(SEQ ID NO: 374)



MKILVAVAVFFLVSTQLFAEEIDANDDLNYWSDWSDSDQIKEAMPEPFEHLLQRIARRK



 


PTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICL


 


SHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCV


 


DCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDRRPKPQQFFGL


 


MGKRDADSSVEKQVALLKALYGHGQISHKRHKTDSFVGLMGKRALNSVAYERSAMQN


 


YERRRK


 


Protachykinin-1 (Substance P, Neurokinin A, Neuropeptide K,


Neuropeptide gamma) Human (amino acid sequence)


(SEQ ID NO: 375)



MKILVALAVFFLVSTQLFAEEIGANDDLNYWSDWYDSDQIKEELPEPFEHLLQRIARRKP



 


TENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLS


 


HIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVD


 


CTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDRRPKPQQFFGLM


 


GKRDADSSIEKQVALLKALYGHGQISHKRHKTDSFVGLMGKRALNSVAYERSAMQNYE


 


RRR


 


Proenkephalin-A Mouse (amino acid sequence)


(SEQ ID NO: 376)



MARFLRLCTWLLALGSCLLATVQAECSQDCAKCSYRLVRPGDINFLACTLECEGQLPSF



 


KIWETCKDLLQVSRPEFPWDNIDMYKDSSKQDESHLLAKKYGGFMKRYGGFMKKMDE


 


LYPMEPEEEANGGEILAKRYGGFMKKDADEGDTLANSSDLLKELLGTGDNRAKDSHQQ


 


ESTNNDEDMSKRYGGFMRSLKRSPQLEDEAKELQKRYGGFMRRKPTENNEDFNIVAVA


 


SNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIP


 


GRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANV


 


QCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDRRVGRPEWWMDYQKRYGGFLKRF


 


AESLPSDEEGENYSKEVPEIEKRYGGFMRF


 


Proenkephalin-A Human (amino acid sequence)


(SEQ ID NO: 377)



MARFLTLCTWLLLLGPGLLATVRAECSQDCATCSYRLVRPADINFLACVMECEGKLPSL



 


KIWETCKELLQLSKPELPQDGTSTLRENSKPEESHLLAKRYGGFMKRYGGFMKKMDEL


 


YPMEPEEEANGSEILAKRYGGFMKKDAEEDDSLANSSDLLKELLETGDNRERSHHQDGS


 


DNEEEVSKRYGGFMRGLKRSPQLEDEAKELQKRYGGFMRRKPTENNEDFNIVAVASNF


 


ATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRC


 


HTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCS


 


DLLKKWLPQRCATFASKIQGQVDKIKGAGGDRRVGRPEWWMDYQKRYGGFLKRFAEA


 


LPSDEEGESYSKEVPEMEKRYGGFMRF


 


Proenkephalin-B Mouse (amino acid sequence)


(SEQ ID NO: 378)



MAWSRLMLAACLLVMPSNVMADCLSLCSLCAVRIQDGPRPINPLICSLECQDLVPPSEE



 


WETCRGFSSFLTLTVSGLRGKDDLEDEVALEEGYSALAKLLEPVLKELEKSRLLTSVPEE


 


KFRGLSSSFGNGKESELAGADRMNDEAAQAGTLHFNEEDLRKQAKRYGGFLRKYPKRK


 


PTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICL


 


SHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCV


 


DCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDKRSSEMARDED


 


GGQDGDQVGHEDLYKRYGGFLRRIRPKLKWDNQKRYGGFLRRQFKVVTRSQENPNTY


 


SEDLDV


 


Proenkephalin-B Human (amino acid sequence)


(SEQ ID NO: 379)



MAWQGLVLAACLLMFPSTTADCLSRCSLCAVKTQDGPKPINPLICSLQCQAALLPSEEW



 


ERCQSFLSFFTPSTLGLNDKEDLGSKSVGEGPYSELAKLSGSFLKELEKSKFLPSISTKENT


 


LSKSLEEKLRGLSDGFREGAESELMRDAQLNDGAMETGTLYLAEEDPKEQVKRKPTEN


 


NEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIK


 


CTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTT


 


GCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDKRYGGFLRKYPKRSS


 


EVAGEGDGDSMGHEDLYKRYGGFLRRIRPKLKWDNQKRYGGFLRRQFKVVTRSQEDP


 


NAYSGELFDA


 


Insulin-like growth hormone 1 (IGF-1) Mouse (amino acid sequence)


(SEQ ID NO: 380)



MGKISSLPTQLFKICLCDFLKIKIHIMSSSHLFYLALCLLTFTSSTTAGPETLCGAELVDAL



 


QFVCGPRGFYFNKPTGYGSSIRRKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPL


 


EVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVD


 


IPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQG


 


QVDKIKGAGGDSSIRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPTKAARSIRAQRHTD


 


MPKTQKSPSLSTNKKTKLQRRRKGSTFEEHK


 


Insulin-like growth hormone 1 (IGF-1) Human (amino acid sequence)


(SEQ ID NO: 381)



MGKISSLPTQLFKCCFCDFLKVKMHTMSSSHLFYLALCLLTFTSSATAGPETLCGAELVD



 


ALQFVCGDRGFYFNKPTGYGSSSRRKPTENNEDFNIVAVASNFATTDLDADRGKLPGKK


 


LPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEA


 


IVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASK


 


IQGQVDKIKGAGGDSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSARSVRAQRH


 


TDMPKTQKYQPPSTNKNTKSQRRKGSTFEERK


 


Insulin-like growth hormone 2 (IGF-2) Mouse (amino acid sequence)


(SEQ ID NO: 382)



MGGSVAGFQVPMGIPVGKSMLVLLISLAFALCCIAAYGPGETLCGGELVDTLQFVCSDR



 


GFYFSRPSSRANRRSRGIVEECCFRSCDLALLETYCATPAKSERDKPTENNEDFNIVAVAS


 


NFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPG


 


RCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQ


 


CSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDERDVSTSQAVLPDDFPRYPVGKFFQY


 


DTWRQSAGRLRRGLPALLRARRGRMLAKELKEFREAKRHRPLIVLPPKDPAHGGASSE


 


MSSNHQ


 


Insulin-like growth hormone 2 (IGF-2) Human (amino acid sequence)


(SEQ ID NO: 383)



MGIPMGKSMLVLLTFLAFASCCIAAYRPSETLCGGELVDTLQFVCGDRGFYFSRPASRVS



 


RRSRGIVEECCFRSCDLALLETYCATPAKSERDKPTENNEDFNIVAVASNFATTDLDADR


 


GKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKES


 


AQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ


 


RCATFASKIQGQVDKIKGAGGDERDVSTPPTVLPDNFPRYPVGKFFQYDTWKQSTQRLR


 


RGLPALLRARRGHVLAKELEAFREAKRHRPLIALPTQDPAHGGAPPEMASNRK


 


Parathyroid hormone (PTH) Mouse (amino acid sequence)


(SEQ ID NO: 384)



MMSANTVAKVMIIMLAVCLLTQTDGKPVRKRKPTENNEDFNIVAVASNFATTDLDADR



 


GKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKES


 


AQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ


 


RCATFASKIQGQVDKIKGAGGDKRAVSEIQLMHNLGKHLASMERMQWLRRKLQDMHN


 


FVSLGVQMAARDGSHQKPTKKEENVLVDGNPKSLGEGDKADVDVLVKSKSQ


 


Parathyroid hormone (PTH) Human (amino acid sequence)


(SEQ ID NO: 385)



MIPAKDMAKVMIVMLAICFLTKSDGKSVKKRKPTENNEDFNIVAVASNFATTDLDADR



 


GKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKES


 


AQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ


 


RCATFASKIQGQVDKIKGAGGDKRSVSEIQLMHNLGKHLNSMERVEWLRKKLQDVHNF


 


VALGAPLAPRDAGSQRPRKKEDNVLVESHEKSLGEADKADVNVLTKAKSQ


 


Parathyroid hormone-related protein (PTHrP) Mouse (amino acid sequence)


(SEQ ID NO: 386)



MLRRLVQQWSVLVFLLSYSVPSRGRSVEGLGRRLKRKPTENNEDFNIVAVASNFATTDL



 


DADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEG


 


DKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKK


 


WLPQRCATFASKIQGQVDKIKGAGGDKRAVSEHQLLHDKGKSIQDLRRRFFLHHLIAEIH


 


TAEIRATSEVSPNSKPAPNTKNHPVRFGSDDEGRYLTQETNKVETYKEQPLKTPGKKKK


 


GKPGKRREQEKKKRRTRSAWPSTAASGLLEDPLPHTSRPSLEPSLRTH


 


Parathyroid hormone-related protein (PTHrP) Human (amino acid sequence)


(SEQ ID NO: 387)



MQRRLVQQWSVAVFLLSYAVPSCGRSVEGLSRRLKRKPTENNEDFNIVAVASNFATTDL



 


DADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEG


 


DKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKK


 


WLPQRCATFASKIQGQVDKIKGAGGDKRAVSEHQLLHDKGKSIQDLRRRFFLHHLIAEIH


 


TAEIRATSEVSPNSKPSPNTKNHPVRFGSDDEGRYLTQETNKVETYKEQPLKTPGKKKKG


 


KPGKRKEQEKKKRRTRSAWLDSGVTGSGLEGDHLSDTSTTSLELDSRRH


 


Osteocalcin Mouse (amino acid sequence)


(SEQ ID NO: 388)



MRTIFLLTLLTLAALCLSDLTDAKPSGPESDKAFMSKQEGNKVVNRLRRKPTENNEDFNI



 


VAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKM


 


KKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKG


 


LANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDRRYLGASVPSPDPLEPTREQC


 


ELNPACDELSDQYGLKTAYKRIYGITI


 


Osteocalcin Human (amino acid sequence)


(SEQ ID NO: 389)



MRALTLLALLALAALCIAGQAGAKPSGAESSKGAAFVSKQEGSEVVKRPRRKPTENNED



 


FNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTP


 


KMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQHAQVDLCVDCTTGCL


 


KGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDRRYLYQWLGAPVPYPDPL


 


EPRREVCELNPDCDELADHIGFQEAYRRFYGPV


 


Urocortin-3 Mouse (amino acid sequence)


(SEQ ID NO: 390)



MLMPTYFLLPLLLLLGGPRTSLSHKFYNTGPVFSCLNTALSEVKKNKLEDVPLLSKKSFG



 


HLPTQDPSGEEDDNQTHLQIKRTFSGAAGGNGAGSTRYRYQSQAQHKGKLYPDKPKSD


 


RGTKKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTR


 


GCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQ


 


VDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDRGTKFT


 


LSLDVPTNIMNILFNIDKAKNLRAKAAANAQLMAQIGKKK


 


Urocortin-3 Human (amino acid sequence)


(SEQ ID NO: 391)



MLMPVHFLLLLLLLLGGPRTGLPHKFYKAKPIFSCLNTALSEAEKGQWEDASLLSKRSFH



 


YLRSRDASSGEEEEGKEKKTFPISGARGGARGTRYRYVSQAQPRGKPRQDTAKSPHRKP


 


TENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANARKAGCTRGCLICLS


 


HIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVD


 


CTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGGDHRTKFTLSLDVPT


 


NIMNLLFNIAKAKNLRAQAAANAHLMAQIGRKK


 


Urocortin-2 Mouse (amino acid sequence)


(SEQ ID NO: 392)



MMTRWALVVFVVLMLDRILFVPGTPIPTFQLLPQNSLETTPSSVTSESSSGTTTGPSASWS



 


NSKASPYLDTRVKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANA


 


RKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEP


 


MEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGG


 


DTRVILSLDVPIGLLRILLEQARYKAARNQAATNAQILAHVGRR


 


Urocortin-2 Human (amino acid sequence)


(SEQ ID NO: 393)



MTRCALLLLMVLMLGRVLVVPVTPIPTFQLRPQNSPQTTPRPAASESPSAAPTWPWAAQ



 


SHCSPTRHPGSRIKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLEVLKEMEANA


 


RKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDIPEIPGFKDLEP


 


MEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQGQVDKIKGAGG


 


DSRIVLSLDVPIGLLQILLEQARARAAREQATTNARILARVGHC


 


Urocortin-1 Mouse (amino acid sequence)


(SEQ ID NO: 394)



MIQRGRATLLVALLLLAQLRPESSQWSPAAAAATGVQDPNLRWSPGVRNQGGGVRALL



 


LLLAERFPRRAGSEPAGERQRRKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKLPLE


 


VLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAIVDI


 


PEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKIQG


 


QVDKIKGAGGDRRDDPPLSIDLTFHLLRTLLELARTQSQRERAEQNRIIFDSVGK


 


Urocortin-1 Human (amino acid sequence)


(SEQ ID NO: 395)



MRQAGRAALLAALLLLVQLCPGSSQRSPEAAGVQDPSLRWSPGARNQGGGARALLLLL



 


AERFPRRAGPGRLGLGTAGERPRRKPTENNEDFNIVAVASNFATTDLDADRGKLPGKKL


 


PLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESAQGGIGEAI


 


VDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQRCATFASKI


 


QGQVDKIKGAGGDRRDNPSLSIDLTFHLLRTLLELARTQSQRERAEQNRIIFDSVGK






Multiplexing Luciferase Reporters for Tracking Multiple Proteins and/or for Normalization
The nucleic acid constructs and propeptide-luciferase fusion proteins described herein can be used either in isolation to track the secretion of a single protein, or in a multiplexed format to track the secretion of multiple proteins simultaneously. The nucleic acid constructs and propeptide-luciferase fusion proteins described herein can be used either in isolation to track the expression of a single protein at the cell surface, or in a multiplexed format to track the expression of multiple proteins at the cell surface simultaneously. As Gaussia luciferase and Cypridina luciferase act on different substrates to create luminescence, one could use a “Propeptide.A—Gaussia luciferase” fusion protein to track secretion of Protein.A, and a “Propeptide.B—Cypridina luciferase” fusion protein to track secretion of Protein.B, if each fusion protein were expressed in the same cells, or if each were expressed separately but in a mixed pool of cells. The ability to multiplex the luciferase reporters has added advantage and utility in chemical and genetic screening assays. Similarly, one could track the cell surface expression of Protein A and Protein B, as described herein.
For example, one skilled in the art could use the multiplex format for screening compounds that affect inflammation. In this example, two nucleic acid constructs encoding propeptide-luciferase fusion proteins can be utilized, the first comprises a propeptide that is an anti-inflammatory cytokine and the Gaussia luciferase and the second comprises a propeptide that is a pro-inflammatory cytokine and the Cypridina luciferase. The nucleic acid constructs are introduced into the same cell, or a population of cells, such that each cell expresses both constructs. The cells are contacted by test compounds and the bioluminescence is detected, for example by luminometer. Luminescence resulting from activation of the Gaussia or Cypridina luciferase indicates that the test compound stimulates anti-inflammatory signaling or pro-inflammatory signaling, respectively. In this manner, more than one biological readout (in this case, mature secreted cytokine) can be detected at one time, granting increased utility to the invention described herein for use in chemical and genetic screens.
In another embodiment, a control luciferase that acts on different substrates to create luminescence from the propeptide-luciferase reporter can be used as an internal reference. For example, as Firefly luciferase acts on a third substrate to produce luminescence, one could use this bioluminescent protein to normalize for expression differences across cells, for example by expressing it constitutively within the cells expressing the propeptide-luciferase fusion reporters described. In such a manner, one could assemble kits to monitor the activity of multiple biologic pathways and/or secreted proteins, with or without normalization to a control luciferase signal, for use in basic science research, as well as chemical and genetic screening projects.
High Throughput Screening Assays
Traditional methods of investigating peptide secretion are time-intensive and expensive. ELISA tests are accurate only within a limited range of detection, and as such, results often need to be verified by using serial dilution assays to verify that the signal detected is within the linear range.
The present invention provides methods that are more efficient, cost-effective, sensitive, accurate, and therefore more amenable to large-scale high throughput chemical and genetic screens than the standard methods known in the art to date. The present invention is highly sensitive and accurate as the detection of the luminescence, for example, by a luminometer, is highly sensitive, requires no dilution, and has a very broad range of signal detection. As an example, up to a thousand-fold dilution of sample still results in a signal within the linear range of the assay.
The present invention contemplates methods for identifying specific modulators, such as chemical compounds or genes, of peptide secretion or cell surface expression using various screening assays known in the art. The present invention also contemplates methods for identifying specific compounds that differentiate a embryonic or iPS stem cell to a mature, or differentiated cell using various screening assays known in the art. For example, the mature or differentiated cell is a hormone-secreting cell, a neuropeptide-secreting cell, a cytokine-secreting cell, or a cell that can no longer differentiate into more than one cell type.
Any screening technique known in the art can be used to screen modulators of peptide secretion or cell surface expression. For example, natural products libraries can be screened using assays of the invention. The present invention contemplates screens for synthetic small molecule agents, chemical compounds, chemical complexes, and salts thereof. Other molecules that can be identified using the screens of the invention include proteins and peptide fragments, peptides, nucleic acids and oligonucleotides, carbohydrates, phospholipids and other lipid derivatives. Other modulators of peptide secretion can also include genes that are involved in regulating the pathways that control hormone secretion. Other modulators of cell surface expression can also include genes that are involved in regulating the pathways that control cell surface expression.
In another aspect, synthetic libraries (Needels et al., Proc. Natl. Acad. Sci. USA 90:10700-4, 1993; Ohlmeyer et al., Proc. Natl. Acad. Sci. USA 90:10922-10926, 1993; Lam et al., International Patent Publication No. WO 92/00252; Kocis et al., International Patent Publication No. WO 9428028) and the like can be used to screen for compounds that modulate peptide secretion.
Test compounds are screened from large libraries of synthetic or natural compounds. Numerous means are currently used for random and directed synthesis of saccharide, peptide, and nucleic acid based compounds. Synthetic compound libraries are commercially available from Maybridge Chemical Co. (Trevillet, Cornwall, UK), Comgenex (Princeton, N.J.), Brandon Associates (Merrimack, N.H.), and Microsource (New Milford, Conn.). A rare chemical library is available from Aldrich (Milwaukee, Wis.). Alternatively, libraries of natural compounds in the form of bacterial, fungal, plant and animal extracts are available from e.g. Pan Laboratories (Bothell, Wash.) or MycoSearch (N.C.), or are readily producible. Additionally, natural and synthetically produced libraries and compounds are readily modified through conventional chemical, physical, and biochemical means (Blondelle et al., Tib Tech, 14:60, 1996).
RNAi and open reading frame (ORF) libraries, such as those of the RNAi Consortium at the Broad Institute, can be used to screen for genes that increase or decrease peptide secretion, or increase or decrease cell surface expression.
The sensitivity and accuracy of the luminescence detection allows for screening at the level of single cell detection. Single cell analysis may be particularly useful in RNAi screens, where pools of RNAi molecules can be tested. For example, a pool of unique short hairpins targeting different genes that each contain an unique “barcode” that designates the target gene, would be introduced to a population of cells that express a prohormone fusion protein of the present invention. The bioluminescence signal and the unique hairpin can be detected using various single cell analysis methods known in the art. For example, the population of cells can be separated and analyzed using various well-, trap-, pattern-, and droplet-based microfluidic devices and platforms. The luminescence signal and the shRNA “barcode” can be determined concurrently or sequentially to identify the genes responsible for modulating hormone secretion.
Once genetic variants that have altered peptide secretion or cell surface expression are identified, additional compound screens can be performed to identify those compounds that reverse or augment the genetic effect.
According to the present invention, a host cell containing a fusion construct between a propeptide and a bioluminescent protein is constructed. Candidate agents are added to in vitro cell cultures of host cells, prepared by known methods in the art, and the activity of the bioluminescent protein is measured within the cellular supernatant. Various in vitro systems can be used to analyze the effects of a new compound on bioluminescent protein expression. For example, light emission is detected by known methods, such as detection with suitable instrumentation (such as a luminometer or CCD camera) in vivo or in vitro, such as in a living cell or intact organism, a cell culture system, a tissue section, or an array. Preferably, the luminescence is detected by luminometer. Preferably, the luminometer is in a plate-reader format.
Vectors and Kits
The present invention also provides a nucleic acid expression vector comprising a nucleic acid sequence encoding a bioluminescent protein, wherein the bioluminescent protein lacks a native signal peptide; a nucleic acid sequence encoding two cleavage sites, wherein the cleavage sites flank the bioluminescent protein such that when the vector is expressed by a cell, the bioluminescent protein is cleaved from the remaining peptide; and at least one insertion site for insertion of a nucleic acid sequence encoding a propeptide such that the inserted nucleic acid sequence is in-frame with the bioluminescent protein. The insertion site is a restriction enzyme site, multiple cloning site containing multiple restriction enzyme sites, or a site recognized by a recombinase. Optionally, the nucleic acid expression vector comprises a promoter, wherein the promoter is operatively linked to the nucleic acid sequence encoding the bioluminescent protein. Optionally, the nucleic acid expression vector comprises a selective marker operatively linked to a second promoter. The selective marker can be an antibiotic resistance gene, drug resistance gene, toxin resistance gene or a cell surface marker.
Alternatively, a nucleic acid expression vector may comprise any nucleic acid construct described herein operatively linked to a promoter and a selective marker operatively linked to a second promoter.
The vectors of the present invention can be expressed in any of the cells described herein.
The present invention further provides a kit. The kit includes any or at least one of the nucleic acid expression vectors described herein, at least one luciferase substrate and instructions for use. The luciferase substrate will be selected according to the nucleic acid expression vector of the kit, such that the luciferase of the expression vector will dictate the luciferase substrate included in the kit. For example, a kit containing a nucleic acid expression vector that encodes Gaussia luciferase will also include the Gaussia luciferase substrate. For those kits that include more than one nucleic acid expression vector, the corresponding luciferase substrates to each nucleic acid expression vector will also be included in the kit.
The present invention further provides a kit that contains any one of the cells that express a propeptide-luciferase fusion protein as described herein.
Any kit of the present invention further comprises a control nucleic acid construct that encodes a control luciferase or cells expressing the control luciferase for use as an internal control. Importantly, the control luciferase is different from the luciferase(s) of the nucleic acid expression vectors; such that the detected luminescence can be easily distinguished from the luminescence signal of the Gaussia and/or Cypridina reporters. For example, the control luciferase is Firefly luciferase.
DEFINITIONS
“Compound” as used herein encompasses all types of organic or inorganic molecules, including but not limited to proteins, peptides, polysaccharides, lipids, nucleic acids, small organic molecules, inorganic compounds, and derivatives thereof.
“Polypeptide,” “Protein,” and “Peptide” are used interchangeably to refer to amino acid chains in which the amino acid residues are linked by covalent peptide bonds. The amino acid chains can be of any length of at least two amino acids, including full-length proteins. Unless otherwise specified, the terms “polypeptide,” “protein,” and “peptide” also encompass various modified forms thereof, including but not limited to glycosylated forms, phosphorylated forms, etc.
“Test agent” or “Test compound” means a chemical compound, preferably an organic compound, to be tested in the present invention to determine its ability to interact with another chemical compound. Test agents may include various forms of organic compounds, or combinations or conjugates thereof. In one embodiment, the test agents preferably are polypeptides, in which case the test agents are termed “test polypeptides” or “test proteins.”
“Fusion construct” refers to a non-naturally occurring hybrid or chimeric construct having two or more distinct portions covalently linked together, each portion being or being derived from a specific molecule. When two or more portions in a fusion construct as defined above are polypeptides and are linked together by peptide bonds, the fusion construct is conveniently referred to as “fusion protein.”
“Preprohormone” is the precursor protein to one or more prohormones, which are in turn precursors to peptide hormones. The protein generally consists of the amino acid chain that is created by the hormone secreting cell, before any changes have been made to it. It contains a signal peptide, the hormone(s) itself (themselves), and intervening amino acids. Before the hormone is released from the cell, the signal peptide and other amino acids are removed.
“Prohormone” is a substance that is a precursor to a hormone.
“Propeptide” is a peptide precursor to a mature peptide. In some embodiments, the propeptide is processed to become a mature peptide, i.e. by cleavage and/or transport through the secretory pathway to be either secreted or expressed at the cell surface.
“Peptide hormones” are a class of peptides that are secreted into the blood stream and have endocrine functions in living animals
“Light-generating” or “luminescent” includes the property of generating light through a chemical reaction or through the absorption of radiation, including phosphorescence, fluorescence, and bioluminescence.
“Bioluminescent proteins” include any light-generating polypeptides, including fluorescent proteins such as green fluorescent protein (GFP) and luminescent proteins such as luciferase.
“Bioluminescent” molecules or moieties include luminescent substances such as proteins that utilize chemical energy to produce light.
“Fluorescent” molecules or moieties include those that are luminescent via a single electronically excited state, which is of very short duration after removal of the source of radiation. The wavelength of the emitted fluorescence light is longer than that of the exciting illumination (Stokes' Law), because part of the exciting light is converted into heat by the fluorescent molecule.
“Light” includes electromagnetic radiation having a wavelength of between about 300 nm and about 1100 nm, but can be of longer or shorter wavelength.
“Light-generating gene product” includes any protein known to those of ordinary skill in the art to provide a readily detectable source of light when present in stable form. Non-limiting examples include light-generating proteins described in U.S. Pat. Nos. 5,683,888, 5,958,713, and 5,650,135, e.g., ferredoxin IV, green fluorescent protein, red fluorescent protein, yellow fluorescent protein, blue fluorescent protein, the luciferase family (see, e.g., W0 03/016839), and the aequorin family. In a preferred embodiment, the light-generating polypeptide moiety is a protein such as green fluorescent protein, red fluorescent protein, yellow fluorescent protein and blue fluorescent protein. Light-generating gene products include light-generating polypeptide moieties.
“Light-generating fusion protein” or “fusion protein’ includes proteins of the invention having a light-generating or luminescent portion, i.e., a light-generating polypeptide such as luciferase and preprohormone.
“Small molecule” includes compositions that have a molecular weight of less than about 5 kD and most preferably less than about 4 kD. Small molecules is, e.g., nucleic acids, peptides, polypeptides, peptidomimetics, carbohydrates, lipids or other organic or inorganic molecules.
“Heterologous gene” includes a gene that has been transfected into a host organism. Typically, a heterologous gene refers to a gene that is not originally derived from the transfected or transformed cells' genomic DNA.
“Recombinant nucleic acid molecules” include nucleic acid sequences not naturally present in the cell, tissue or organism into which they are introduced.
The term “operably linked” relates to the orientation of polynucleotide elements in a functional relationship. Operably linked means that the DNA sequences being linked are generally contiguous and, where necessary to join two protein coding regions, contiguous and in the same reading frame. However, since enhancers generally function when separated from the promoter by several kilobases, some nucleic acids is operably linked but not contiguous.
The terms “polynucleotide” and “nucleic acid molecule” are used interchangeably to refer to polymeric forms of nucleotides of any length. The polynucleotides may contain deoxyribonucleotides, ribonucleotides and/or their analogs. Nucleotides may have any three-dimensional structure, and may perform any function, known or unknown. The term “polynucleotide” includes single-, double-stranded and triple helical molecules. “Oligonucleotide” refers to polynucleotides of between 5 and about 100 nucleotides of single- or double-stranded DNA. Oligonucleotides are also known as oligomers or oligos and are isolated from genes, or chemically synthesized by methods known in the art. A “primer” refers to an oligonucleotide, usually single-stranded, that provides a 3′-hydroxyl end for the initiation of enzyme-mediated nucleic acid synthesis. The following are non-limiting embodiments of polynucleotides: a gene or gene fragment, exons, introns, mRNA, tRNA, rRNA, ribozymes, cDNA, recombinant polynucleotides, branched polynucleotides, plasmids, vectors, isolated DNA of any sequence, isolated RNA of any sequence, nucleic acid probes and primers. A nucleic acid molecule may also comprise modified nucleic acid molecules, such as methylated nucleic acid molecules and nucleic acid molecule analogs. Analogs of purines and pyrimidines are known in the art, and include, but are not limited to, aziridinycytosine, 4-acetylcytosine, 5-fluorouracil, 5-bromouracil, 5-carboxymethylaminomethyl-2-thiouracil, 5-carboxymethyl-aminomethyluracil, inosine, N6-isopentenyladenine, 1-methyladenine, 1-methylpseudouracil, 1-methylguanine, 1-methylinosine, 2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytosine, 5-methylcytosine, pseudouracil, 5-pentylnyluracil and 2,6-diaminopurine. The use of uracil as a substitute for thymine in a deoxyribonucleic acid is also considered an analogous form of pyrimidine.
A “fragment” of a polynucleotide is a polynucleotide comprised of at least 9 contiguous nucleotides, preferably at least 15 contiguous nucleotides and more preferably at least 45 nucleotides, of coding or non-coding sequences.
The term “homologous recombination” refers to the exchange of DNA fragments between two DNA molecules or chromatids at the site of homologous nucleotide sequences.
The term “homologous” as used herein denotes a characteristic of a DNA sequence having at least about 70 percent sequence identity as compared to a reference sequence, typically at least about 85 percent sequence identity, preferably at least about 95 percent sequence identity, and more preferably about 98 percent sequence identity, and most preferably about 100 percent sequence identity as compared to a reference sequence. Homology is determined using, for example, a “BLASTN” algorithm. It is understood that homologous sequences can accommodate insertions, deletions and substitutions in the nucleotide sequence. Thus, linear sequences of nucleotides are essentially identical even if some of the nucleotide residues do not precisely correspond or align. The reference sequence is a subset of a larger sequence, such as a portion of a gene or flanking sequence, or a repetitive portion of a chromosome.
The term “transgenic cell” refers to a cell containing within its genome a nucleic acid encoding a preprohormone operably linked to a nucleic acid encoding a bioluminescent protein introduced by the method of gene targeting.
The term “proliferating cell” includes any cell undergoing cell division.
As used herein, the terms “selectable marker” and “positive selection marker” refer to a gene encoding a product that enables only the cells that carry the gene to survive and/or grow under certain conditions. For example, plant and animal cells that express the introduced neomycin resistance (Neo (r)) gene are resistant to the compound G418. Cells that do not carry the Neo (r) gene marker are killed by G418. Other positive selection markers are known to or are within the purview of those of ordinary skill in the art.
A “host cell” includes an individual cell or cell culture that is or has been a recipient for vector(s) or for incorporation of nucleic acid molecules and/or proteins. Host cells include progeny of a single host cell, and the progeny may not necessarily be completely identical (in morphology or in total DNA complement) to the original parent due to natural, accidental, or deliberate mutation. A host cell includes cells transfected with the constructs of the present invention.
The term “modulates” as used herein refers to the decrease, inhibition, reduction, increase, or enhancement of a gene function, expression, or activity.
EXAMPLES
Example 1: General Methods
Cell Culture
MIN6 cells (Miyazaki, J. et al Endocrinology 127:126-132 (1990)) were maintained in DMEM with 4.5 g/L glucose, supplemented with 10% heat-inactivated fetal bovine serum and 55 μM beta-mercaptoethanol (Sigma, St. Louis, Mo.). INS-1E cells (Merglen, A et al. Endocrinology 145:667-678 (2004)) were maintained in RPMI with 2 g/L glucose, supplemented with 10% heat-inactivated fetal bovine serum, 1 mM sodium pyruvate, 2 mM L-glutamine, 10 mM HEPES and 55 μM beta-mercaptoethanol (Invitrogen, Grand Island, N.Y.). Cells were grown on standard tissue culture-treated plastic (Becton Dickenson, Waltham, Mass.) and all dissociation steps were performed using TrypLE (Invitrogen).
Prohormone-Luciferase Constructs
The proinsulin-luciferase fusion construct was created by Gibson Assembly in the pUC19 vector (NEB, Ipswich, Mass.) using two gBlocks (IDT, Coralville, Iowa) encoding the protein and Gibson Assembly Master Mix (NEB). Mouse proamylin was subcloned from the vector pT7T3D-PacI (clone MmCD00310704, DF/HCC DNA Resource Core, Boston, Mass.) into the pCI-Neo vector (Promega, Madison, Wis.). Gaussia luciferase was amplified from pCMV-GLuc (NEB) using oligonucleotide primers (IDT) designed to exclude the start codon, signal peptide and stop codon, and to add a prohormone convertase 2 site to the c-terminus and flanking BsmI sites. pCI-Neo-proamylin was then digested with BsmI (NEB) and ligated to the BsmI digest of the Gaussia luciferase PCR product. The proinsulin-luciferase and proamylin-luciferase fusion constructs were subsequently PCR-amplified with primers to add attB1 and attB2 sites, inserted by BP Clonase II into the Gateway Entry vector pDONR223 (Invitrogen), and then shuttled by LR Clonase II into the Gateway Destination vector pLX304 (David Root lab, Addgene plasmid 25890) (Yang, X. et al. Nat Methods 8:659-661 (2011)). Other prohormone-luciferase constructs, such as the preproglucagon-luciferase construct, were generated in a similar manner.
Lentivirus Production
Lentivirus expressing each prohormone-luciferase fusion protein was produced as described (Yang, X. et al. Nat Methods 8:659-661 (2011)). Briefly, 2 μg of pLX304 expression plasmid containing the fusion construct, 1.8 μg of psPAX2 packaging plasmid (Didier Trono lab, Addgene plasmid 12260), 200 ng of pMD2.G envelope plasmid (Didier Trono lab, Addgene plasmid 12259) and 12 μL TransIT-LT1 (Minis Bio, Madison, Wis.) were used to transfect a 10 cm dish of HEK293T packaging cells (ATCC, Manassas, Va.). Virus was pooled from harvests at 48 and 72 hours and passed through 0.2 μm cellulose acetate filters (VWR, Radnor, Pa.) prior to use.
Virus Infection
MIN6 and INS-1E cells were plated in their respective growth media with the addition of 8 μg/mL polybrene (Sigma). Virus was then added and the cells were spun at 800 g for 1 hour at 30° C. After 24 hours in the presence of virus, the cells were placed in fresh growth media for 24 hours, then selection was performed using 5 μg/mL blasticidin (Invitrogen). Cells were assessed for luciferase localization and secretion after 1 week of antibiotic selection.
Immunofluorescence
Staining of cells was performed as described (Walpita D, et al. J Biomol Screen 17:509-518 (2012)). Briefly, cultures were fixed for 15 min at room temperature using 3% paraformaldehyde and washed twice with PBS. Cells were permeabilized for 20 min at room temperature in PBS containing 0.1% saponin and blocked overnight at 4° C. with 2% bovine serum albumin (BSA) in PBS. Primary antibodies to Gaussia luciferase (#401P, Nanolight, Pinetop, Ariz.) and insulin (#18510, Sigma) were diluted 1:1000 in antibody dilution buffer (ADB; 1% BSA in PBS) and incubated overnight at 4° C., followed by three washes in ADB. Cultures were then incubated in secondary antibodies (Alexa Fluor-conjugated anti-rabbit and anti-guinea pig; Invitrogen) diluted 1:1000 in ADB for 1 hour at room temperature, followed by five washes with PBS. Images were acquired using a Zeiss Axiovert 200M inverted fluorescent microscope and AxioCam MRm camera (Zeiss, Thornwood, N.Y.).
Secretion Assays
For standard secretion assays, MIN6 and INS-1E cells were plated in 96-well format at a density of 4×104 cells per well in 100 μL of their respective growth media and incubated overnight at 37° C. in 5% CO2 to allow attachment. Cells were washed once with PBS and incubated for 1 hour at 37° C. in sterile, 0.45 μm-filtered Krebs Ringer Buffer (KRB) containing 138 mM NaCl, 5.4 mM KCl, 2.6 mM MgCl2, 2.6 mM CaCl2, 5 mM NaHCO3, 10 mM HEPES and 5 g/L bovine serum albumin (Sigma). The cells were then washed in KRB once and stimulated for 1 hour in 100 μL of fresh KRB containing varying amounts of glucose and compounds. The insulin concentration was determined using an insulin ELISA (ALPCO) with the supplied protocol. Luciferase activity was determined from the same samples by adding the coelenterazine substrate (Nanolight) to the supernatant to a final concentration of 20 μM and reading on a standard plate reader (Biotek, Winooski, Vt.).
High-Throughput Screens
MIN6 and INS-1E cells were expanded to 80% confluence in their respective growth media, washed once in PBS and incubated for 1 hour at 37° C. in KRB without glucose. Cells were then dissociated, spun at 300 g×2 minutes, resuspended in fresh KRB without glucose and filtered through 40 μm mesh (Becton Dickenson). Next, the cells were counted and diluted in KRB to 1×10^6 cells per mL, and glucose was added as required for each experiment. Cells were then seeded in 384-well format, 3×10^4 cells in 30 μL per well, using a Multidrop Combi device (Thermo Scientific, Billerica, Mass.). Compounds from the Pharmakon 1600 Collection (Microsource Discovery Systems, Gaylordsville, Conn.) were pinned into each well using a CyBio Vario (CyBio, Jena, Germany) to a final concentration of 30 μM, and the plates were then incubated for 1 hour at 37° C. The plates were then spun at 300 g×2 minutes and the supernatant transferred to a new 384-well plate. Coelenterazine substrate was then added to a final concentration of 20 μM and luciferase activity determined using a standard plate reader (Biotek).
Data Analysis
For the high-throughput screens, the Z-score of each test compound was calculated using the formula:




  
    Z
    -
    score
  
  =
  
    
      x
      -
      μ
    
    σ
  




where x is the log-transformed, signal decay-adjusted luciferase signal from a compound-treated well, μ is the mean of the log-transformed luciferase signals from the DMSO-treated wells on the same plate, and σ is the standard deviation of the log-transformed luciferase signals of the DMSO-treated wells across all plates (to allow for cross-plate comparison of compounds). Prior to calculating the Z-score, a row-based correction factor was applied to all luciferase readings to adjust for the rapid signal decay occurring during the course of each 384-well plate read. The signal decay was modeled as a logarithmic function (Microsoft Excel) and then used to adjust each well's luciferase reading based on the corresponding row within the plate.
Human Islet Cell Culture
Human islets were obtained through the Integrated Islet Distribution Program (http://iidp.coh.org/). Specific donor information is reported in Supplemental Table S2. Islets were maintained and dissociated in 96-well format as described4. To test compounds for their effects on insulin secretion, the dissociated islets were washed gently in KRB and incubated for 1 hour at 37° C. in KRB without glucose. The cells were then washed twice with KRB and placed in 100 μL of fresh KRB with 50 mg/dL glucose. Compounds were added to a final concentration of 50 μM and the cells were treated for 1 hour at 37° C. Thereafter, the supernatant was removed and the insulin concentration measured using an insulin ELISA (ALPCO) according to the manufacturer's instructions.
Example 2: Construction of Proamylin Fusion Proteins to Measure Amylin Secretion
The key concept of our creation is that luciferase, when appropriately targeted to the secretory granule of a beta cell, can be used as a close proxy for insulin in a secretion assay. To target luciferase to the secretory granule of the beta cell, we created novel fusion proteins in which the sequence encoding Gaussia luciferase was placed within the open reading frame of proamylin, a peptide hormone that are normally co-secreted from the beta cell. Gaussia luciferase was chosen rather than a more commonly used luciferase because of its smaller size and more intense luminescence characteristics (1000 times brighter than Firefly luciferase). The construct showed robust luciferase response without any detrimental effect on beta cell viability or replication.
Amylin comprises about 1% of the protein in each secretory vesicle of the beta cell, and it is derived from a prohormone that undergoes cleavage by the same enzymes that convert proinsulin to mature insulin within these vesicles. Amylin differs from insulin in its smaller size, simplified protein structure and fewer cysteine residues involved in disulfide bridge formation. In our construct, we selected the mouse isoform of proamylin because it does not form toxic amyloid protein aggregates, unlike the human isoform. As shown in FIG. 1A, the luciferase was placed near the C-terminal end of the proamylin peptide, adjacent to an existing “prohormone convertase 2” (PC2) cleavage site. To increase the likelihood that the luciferase would function well after its release from the proamylin peptide, we added 6 bases encoding an additional PC2 cleavage site to the 3′-end of the luciferase sequence. As a result, the cleaved luciferase protein differed from the wildtype luciferase by only 3 additional amino acids located on its N-terminus, with no additional amino acids on its C-terminus.
Example 3: Measurement of Insulin Secretion Using Preproamlyin Fusion Constructs
The preproamlyin constructs was introduced into the MIN6 mouse beta cell line (FIGS. 3A and 3B). High magnification imaging studies by immunohistochemistry shows co-localization the luciferase protein with insulin in the secretory granules, as expected (FIG. 1B). Cells containing the reporter secreted luciferase in response to glucose and other secretagogues in close correlation with insulin, as measured by the standard ELISA (FIG. 2). In studies performed to date, the secreted luciferase appears to serve as a close proxy for secreted insulin (FIG. 4). Specifically, negative controls show treatment with DMSO and glipizide even at high concentrations did not increase luciferase activity. Stimulation of cells with IBMX, forskolin, KCl, and PMA increased luminescence in a dose-dependent manner, indicating that the secreted luciferase can be used as an accurate readout of amylin or insulin secretion.
Example 4: Construction of Proglucagon Fusion Protein to Measure Glucagon and Glp-1 Secretion
The glucagon gene contains sequences encoding 4 peptide hormones: (a) Grpp, (b) glucagon, (c) Glp-1, and (d) Glp-2 (FIG. 5). Preglucagon processing results result in glucagon or Glp-1 secretion. The construct described herein allows for detection of glucagon or Glp-1 secretion. A preproglucagon-luciferase (G2) reporter was constructed using mouse glucagon, which contains endogenous PC1 and PC2 sites that are utilized to produce glucagon or Glp-1. The G2 construct was constructed similarly to the proinsulin and proamylin constructs described supra. The Gaussia luciferase gene was inserted near the C-terminal end of the proglucagon peptide, between the Glp-1 and Glp-2-encoding peptides Importantly, both PC1 and PC2 cleavage sites were added to the N-terminal and C-terminal ends of the Gaussia luciferase. The cellular context determines which cleavage sites are utilized for glucagon processing. For example, introduction of the G2 construct into PC1-expressing L cells would result in cleavage at the PC1 sites for Glp-1 secretion, while introduction of the G2 construct into PC2-expressing alpha cells would result in cleavage at the PC2 sites for glucagon secretion.
Example 5: Measuring Glucagon and Glp-1 Secretion
The preglucagon G2 construct was introduced into the Alpha TC1 mouse alpha cell line. Cells were stimulated with IBMX, arginine, or potassium chloride (KCl) and varying amounts of glucose in the range of 0-300 mg/dL (0-16.7 mM). Luciferase activity of the supernatant was determined (FIG. 6). As expected, the G2-expressing alpha cells secreted luciferase in response to arginine and KCl1 in a glucose-dependent manner. There was no secretion of luciferase in response to IBMX, also as expected. This assay demonstrates that the secreted luciferase appears to serve as a close proxy for secreted glucagon.
The proglucagon G2 construct was also introduced into the GLUTag mouse L-cell line. Cells were stimulated with Forskolin/IBMX, KCl, glipizide, PMA, and L-glutamine. Luciferase activity of the supernatant was determined. As expected, the G2-expressing L-cells secreted luciferase in response to forskolin/IBMX, Kcl and PMA; and also as expected, there was no secretion of luciferase in response to glipizide or L-glutamine (FIG. 7A). Glp-1 secretion was measured by standard ELISA and results show that luciferase secretion is closely correlated with Glp-1 secretion (FIG. 7B). Taken together, these assay results demonstrate that the secreted luciferase serves as a close proxy for secreted Glp-1. The G2 reporter was also tested in L-cells in 384-well format, to demonstrate the amenability of the reporter in a high throughput format.
Example 6: Construction of Proinsulin Fusion Proteins to Measure Insulin Secretion
To enable high-throughput investigation of genes and compounds affecting insulin secretion, a simple, reproducible, and much less expensive assay was needed. We reasoned that luciferase could be used to create such an assay, if the enzyme could be properly targeted to secretory vesicles in the beta cell via the natural proinsulin processing pathway. To test this hypothesis, we constructed a proinsulin-luciferase fusion protein in which we placed the sequence of Gaussia luciferase within the C-peptide portion of proinsulin (FIG. 8A), a fragment that is normally cleaved by prohormone convertases and co-secreted with mature insulin during exocytosis (Oyer, P. E. et al. J Biol Chem 246:1375-1386 (1971)).
As shown in FIG. 8A we placed the luciferase within the c-peptide portion of proinsulin, and near the C-terminal end of the proamylin peptide adjacent to an existing “prohormone convertase 2” (PC2) cleavage site. To increase the likelihood that the luciferase would function well after its release from each prohormone, we added PC2 cleavage sites to both ends of the luciferase using in the proinsulin construct, and to the 3′-end of the luciferase used in the proamylin construct. After cleavage, the luciferase protein will therefore differ only minimally from the wildtype luciferase protein.
We envisioned that luciferase would remain trapped within proinsulin until cleavage of the fusion protein by pH-sensitive prohormone convertases resident in secretory vesicles, enabling its co-secretion with mature insulin after stimulation of the beta cell.
Upon expression of the construct under the control of a constitutive promoter in rodent beta-cell lines (Miyazaki, J. et al. Endocrinology 127:126-132 (1990); Merglen, A. et al. Endocrinology 145:667-678 (2004)), we observed strong co-localization of insulin and luciferase within the secretory granules of most cells (FIG. 8B). Challenging the cells with increasing glucose concentrations induced secretion of both luciferase and insulin, as measured by ELISA, in close correlation (r2=0.996) (FIG. 8C). Furthermore, upon treatment of the cells with established insulin secretagogues, luciferase secretion tracked closely with insulin in each instance (FIG. 8D).
Example 7: High Throughput Screening Using the Proinsulin Fusion Protein
We next sought to determine the potential of the proinsulin-luciferase reporter cell line for high-throughput screening by optimizing the assay for 384-well format, where it exhibited tight reproducibility (CV<5%) and excellent separation between positive and negative controls (Z′ factor 0.6) (Zhang, J. H. et al. J Biomol Screen 4:67-73 (1999)). We performed a pilot chemical screen at a substimulatory glucose concentration (100 mg/dL) in MIN6 cells using a collection of 1600 known bioactive small molecules, with the sulfonylurea glipizide included as a positive control. Each compound was tested in duplicate, and we observed high reproducibility within experimental replicates (R2=0.80) (FIG. 9A). Several commonly used drugs without known links to beta-cell function caused significant increases in luciferase secretion in our assay (compounds summarized in Table 1).







TABLE 1







Top insulin secretagogues from 1600 compound screen, active in


both the presence and absence of glucose.









Compound
Z-score
Annotation





Triamterene 
76.3
Potassium-sparing diuretic


Tyrothricin
52.4
Topical antibiotic


Trihexyphenidyl
50.5
Anticholinergic


Levosimendan
40.7
Calcium sensitizer used in CHF


Tacrine
38.8
Cholinesterase inhibitor


Monobenzone
38.7
Decreases melanin excretion


(S)-chlorpheniramine 
31.8
Antihistamine


Orphenadrine
27.4
Anticholinergic


Nafronyl
25.4
Serotonin-R antagonist


Camylofine
23.8
Anticholinergic









To confirm that the results from the luminescent assay were reflective of insulin secretion, we treated MIN6 cells with the top hits from the primary screen and measured insulin secretion using the standard ELISA. All top-scoring compounds increased insulin secretion, some to a level higher than that of our positive control, glipizide (FIG. 9B). Next, to determine if our assay could identify compounds with relevance to human beta-cell function, we tested the top compounds for effects on insulin secretion at 50 mg/dL glucose using dissociated human pancreatic islet cells (Table 2) (Walpita, D. et al. J Biomol Screen 17:509-518 (2012)).







TABLE 2





Summary of human islet information.


















Age:
54 years-old



Gender:
male



Height:
 77 inches



Weight:
278 pounds



BMI:
33



Islet purity:
85%



Islet viability:
93%



Cause of death:
cerebrovascular accident (stroke)










Six of the eight compounds augmented insulin secretion from human beta cells to a similar extent as glipizide (FIG. 9C).
As there is a well-recognized need for glucose-dependent small-molecule agonists of insulin secretion, we subsequently evaluated whether our assay could be adapted to find such secretagogues. We repeated the 1600-compound screen in the presence and absence of a substimulatory glucose concentration (100 mg/dL). As expected, the sulfonylurea glipizide caused luciferase secretion regardless of the ambient glucose level, while several compounds appeared to have the desired property of increasing luciferase secretion only in the presence of glucose (FIG. 9D). We confirmed that these compounds augment insulin secretion only in the presence of glucose using the standard ELISA in MIN6 cells (FIG. 9E). Notably, none of the top-scoring compounds in this screen have been previously reported to affect insulin secretion (Table 3).







TABLE 3







Top glucose-dependent insulin secretagogues from 1600 compound 


screen, active only in the presence of a permissive glucose environment.










Z-score













With 
Without





Glucose
Glucose
Δ
Annotation














Monobenzone
31.8
5.6
26.2
Decreases melanin 






secretion


Bergapten
20.9
2.9
18
Citrus product, alters K 






currents


Hydroxyprogesterone
18.3
2.7
15.6
Steroid hormone


Nafronyl
17.8
3.4
14.4
Serotonin-R antagonist


Clemizole
12.2
0.4
11.8
Antihistamine


Spiperone
9.0
−2.8
11.8
Serotonin-R, DA-R 






antagonist


Propafenone
10.5
1.5
9
Na-channel, beta blocker


Carvedilol
10.2
1.5
8.7
Alpha-1, beta blocker


Camylofine
4.7
0.2
4.5
Antimuscarinic









As compared to the standard ELISA, our luminescent insulin secretion reporter assay has the advantages of simplicity, at least a 50-fold decrease in cost, and reproducibility in high-throughput screening applications. After stimulation of beta cells expressing the fusion protein, luciferase activity in the supernatant is determined by simply adding the luciferase substrate coelenterazine and measuring the resulting luminescence with a plate reader. No wash steps are required and the procedure takes less than one minute to complete per 384-well plate.
Much like an ELISA, our assay detects changes in the secretion of insulin rather than in its expression. Other groups have described reporters in which a luciferase is placed under the control of the insulin promoter (Olansky, L. et al. J Clin Invest 89:1596-1602 (1992)), and while these are useful for tracking gene transcription, they are not applicable to screens of insulin release as the transcriptional activity of insulin correlates poorly with its secretion.
In summary, we have created a fast and inexpensive luminescent assay to accurately and reproducibly detect insulin secretion in high-throughput screening applications. In addition to chemical screening, this reporter should prove useful for investigating (1) genes impacting beta-cell function, (2) physiologic stressors relevant to diabetes, and (3) culture conditions promoting differentiation of stem cells into insulin-secreting beta cells. More generally, our prohormone-luciferase fusion design may prove useful to monitor the secretion of other disease-relevant peptide hormones for which ELISA-based methods are even more limited, including glucagon, GLP-1, and peptide YY. Such luminescent hormone secretion reporters would represent valuable tools in the search for drugs to treat metabolic and endocrine disorders.
As described supra, high throughput screening of compounds that modulate other hormone secretion can also be performed. For example, screening of compounds that modulate, increase, or decrease secretion of amylin, glucagon, or Glp-1 can be performed using the fusion protein constructs described herein and the methods described supra.
Furthermore, the methods demonstrated supra can also be used for high throughput screening of compounds that modulate peptide secretion. For example, screening of compounds that modulate, increase, or decrease secretion of peptides such as neuropeptides and cytokines can be performed using the fusion protein constructs described herein. The methods demonstrated supra can also be used for high throughput screening of compounds that modulate cell surface expression of transmembrane proteins or peptides. For example, screening of compounds that modulate, increase, or decrease the cell surface expression of transmembrane proteins can be performed using the fusion protein constructs described herein.
Example 8: Screening for Genes that Modulate Peptide Secretion or Cell Surface Expression
The hormone-luciferase constructs of the invention can be used in high throughput screens to find genes that significantly alter the secretion of hormones of interest, for example insulin (in response to glucose), amylin, glucagon or Glp-1.
We are currently using our proinsulin construct in the MIN6 and INS-1E beta cell lines to perform a pilot RNAi screen of genes known to impact insulin secretion. The effect of silencing the expression of these positive control genes will be assessed using the luciferase readout. Ample negative control hairpins will also be included in this pilot study to characterize the baseline variability of luciferase secretion. Once the assay is validated for use with RNAi constructs, a targeted RNAi screen can be performed against genes recently implicated by human genetics in the pathogenesis of type 2 diabetes and intermediate traits, such as fasting glucose.
Genome wide association studies have identified over 40 sequence variants linked to this disease, many of which affect insulin levels after a glucose challenge, yet in most cases the risk polymorphism is non-coding and thought to impart risk for disease through changes in the expression of a nearby gene. We will cast a wide net by testing RNAi constructs against all genes within +/−300 kilobases from each risk variant, or about 120 genes in total. The RNAi Consortium at the Broad has created 5-10 hairpins targeting each of these genes, and we will test each available construct for an effect on insulin secretion using our luciferase-based assay. “Hits” from this primary screen will be confirmed through repeat testing with our assay, and then validated in an independent assay using a commercially available insulin ELISA.
Pending the results from this targeted RNAi screen, we anticipate performing an unbiased genome wide RNAi screen to comprehensively explore the genes regulating insulin secretion in the beta cell. Should a human beta cell line become available, we will validate our construct in this line and then use it to perform the RNAi screen using the Broad's human lentiviral shRNA library.
Other propeptide-luciferase constructs of the present invention can also be utilized in assays to determine genes that modulate peptide secretion or cell surface expression of the peptide.
Example 9: Screening for Compounds that Modulate Peptide Secretion or Cell Surface Expression
Our invention allows for the high throughput measurement of hormone secretion in the setting of genetic and chemical perturbations, and as such is well suited to screens for compounds impacting this physiologic process. The hormone-luciferase constructs of the invention can be used in small molecule cell-based screens to find compounds that significantly alter the secretion of hormones of interest, for example insulin (in response to glucose), amylin, glucagon or Glp-1.
As described in Example 7, we have performed a pilot screen using the proinsulin fusion protein to screen 1600 known bioactive small molecules, which identified several compounds that increase insulin secretion in a glucose-dependent manner. Based on these results, we were selected to participate in the NIH MLPCN program, which will allow us to screen >350,000 compounds for similar effects on insulin secretion.
Similarly, any of the fusion proteins of the present invention can be used in screens for compounds that modulate hormone secretion. Pilot screens will be performed involving 6 plates of compounds enriched for known bioactive molecules. Based on the results from this initial screen, we anticipate testing a larger collection of compounds to fully explore the interaction of small molecules with the pathways regulating insulin secretion. Targets of any compounds identified in these screens will be sought using the tools of the Chemical Biology and Proteomics groups at the Broad, so as to determine the relevant molecular pathways.
As described in Example 8, genes that regulate hormone secretion can be identified using the described invention. Once genetic variants are identified that adversely affect insulin secretion, we will screen small molecules for their ability to reverse or modulate this genetic effect, so as to increase our understanding of the underlying biology.
Furthermore, genes that regulate secretion of other peptides, such as neuropeptides and cytokines can also be identified using the described invention. Similarly, genes that regulated the cell surface expression of transmembrane proteins can also be identified using the described invention. Similarly, once genetic variants are identified that adversely affect peptide secretion or cell surface expression, small molecules can then be screened for their ability to reverse or modulate the genetic effect.
Example 10: Screening for Factors that Differentiate Cells
In addition to using the hormone reporter constructs for screening compounds or genes that modulate and peptide secretion, the present invention provides a method for screening for factors that can differentiate iPS cells or ES cells into pancreatic beta cells competent for hormone secretion (FIG. 10). The present invention also provides a method for screening for factors that can differentiate iPS or ES cells into any differentiated or mature cell that is capable of peptide-secretion or cell surface expression of the particular peptide of interest. ES or iPS cells are infected with any of the reporter constructs of the present invention, and can be differentiated using methods known in the art. Luciferase secretion is used as a marker of cell maturation in screens to identify compounds (such as small molecules) or other factors that induce cell differentiation and maturation. As an initial experiment, human beta-like cells were infected with the proinsulin reporter construct. Luciferase activity was detected as described supra, and insulin secretion was measured by standard ELISA (FIG. 11). Results indicate that the luciferase signal and secreted insulin were closely correlated, therefore demonstrating that the reporter construct can be used as a marker for beta cell maturation.
One of ordinary skill in the art can use this example as guidance to screen factors that differentiate iPS or ES cells into a differentiated or mature cell.

## Claims

1. A nucleic acid construct comprising a nucleic acid molecule comprising a sequence encoding a propeptide-bioluminescent fusion protein for expression in a cell
wherein:
the propeptide is a precursor to a mature peptide and the mature peptide is secreted or expressed at the cell surface,
the propeptide-bioluminescent fusion protein comprises the bioluminescent protein and the propeptide wherein the bioluminescent protein is inserted into the propeptide,
the bioluminescent protein comprises two cleavage sites, the first of which is positioned no more than four amino acids from the N-terminal end of the bioluminescent protein and the second of which is positioned no more than four amino acids from the C-terminal end of the bioluminescent protein, and
when the propeptide-bioluminescent fusion protein is expressed by the cell, the bioluminescent protein is cleaved from the propeptide and the bioluminescent protein is secreted simultaneously with the secretion of the mature peptide, or
when the bioluminescent-fusion protein is expressed by the cell, the bioluminescent protein is cleaved from the propeptide simultaneously upon expression of the mature peptide at the cell surface.

2. The nucleic acid construct of claim 1, wherein the propeptide is a prohormone, a preprohormone, a cytokine precursor or a neuropeptide precursor.

3. The nucleic acid construct of claim 1, wherein the bioluminescent protein comprises less than 200 amino acids.

4. The nucleic acid construct of claim 2, comprising any one of the nucleotide sequences of SEQ ID NO: 5-100.

5. The nucleic acid construct of claim 2, wherein the preprohormone is preproinsulin and the bioluminescent protein is within the C-peptide component of preproinsulin.

6. The nucleic acid construct of claim 2, wherein the preprohormone is preproamylin and the N-terminal end of the bioluminescent protein is inserted between the regions encoding the signal peptide and the mature amylin hormone or between the mature amylin hormone and the C-terminus of the molecule.

7. The nucleic acid construct of claim 2, wherein the preprohormone is preproglucagon and the bioluminescent protein is inserted between the regions encoding the GRPP and glucagon, glucagon and GLP-1, or GLP-1 and GLP-2.

8. The nucleic acid construct of claim 1, wherein the sequence encoding the first or second cleavage site or the first and second cleavage sites has a sequence selected from the group consisting of SEQ ID NOs: 101-138.

9. The nucleic acid construct of claim 2, further comprising a sequence encoding an additional cleavage site in the fusion protein at the N-terminal and C-terminal ends of the bioluminescent protein.

10. The nucleic acid construct of claim 1, further comprising at least 3 additional nucleotides flanking the DNA encoding at least one cleavage site.

11. The nucleic acid construct of claim 1, further comprising a promoter.

12. The nucleic acid construct of claim 1, wherein the bioluminescent protein is a luciferase.

13. The nucleic acid construct of claim 12, wherein the luciferase is a Gaussia luciferase.

14. The nucleic acid of construct of claim 13, wherein the Gaussia luciferase lacks a native signal sequence.

15. The nucleic acid construct of claim 14, wherein the Gaussia luciferase comprises SEQ ID NO: 1.

16. The nucleic acid construct of claim 12, wherein the luciferase is a Cypridina luciferase.

17. The nucleic acid of construct of claim 16, wherein the Cypridina luciferase lacks a native signal sequence.

18. The nucleic acid construct of claim 17, wherein the Cypridina luciferase comprises SEQ ID NO: 3.

19. A cell comprising a nucleic acid construct of claim 1, wherein the cell is capable of expressing the encoded propeptide-bioluminescent fusion protein.

20. The cell of claim 1, wherein the cell is an endocrine cell, an immune cell, a neuron, a hepatocyte, a myocyte, a kidney cell, an adipocyte, an osteocyte or a cell line derived therefrom.

21. The cell of claim 19, wherein the cell is an endocrine cell, and the endocrine cell is a beta cell, an alpha cell, an L cell, a K cell, another endocrine cell, or a cell line derived from any of the foregoing endocrine cells.

22. The cell of claim 20, wherein the immune cell is a B cell, a T cell, a monocyte, a macrophage, a dendritic cell, a mast cell, a neutrophil or a cell line derived from any of the foregoing immune cells.

23. A cell comprising at least two nucleic acid constructs of claim 1, wherein the cell is capable of expressing the encoded fusion protein and wherein the bioluminescent proteins of the at least two nucleic acid constructs are different.

24. The cell of claim 23, wherein the first nucleic acid construct comprises a Gaussia luciferase and wherein the second nucleic acid construct comprises a Cypridina luciferase.

25. The cell of claim 19, wherein the cell further comprises a control nucleic acid construct encoding a control luciferase as an internal reference, wherein the cell is capable of expressing the control luciferase.

26. A nucleic acid expression vector comprising a nucleic acid construct of claim 1 operatively linked to a promoter and a selective marker operatively linked to a second promoter.

27. A kit comprising a cell of claim 19, and instructions for use.

28. The kit of claim 27, further comprising a control nucleic acid construct encoding a control luciferase as an internal reference.

29. The nucleic acid construct of claim 1,
comprising any one of the nucleotide sequences of SEQ ID NO: 139-330, or
encoding any one of the propeptide-bioluminescent fusion protein sequences of SEQ ID NO: 331-395, or
encoding a propeptide-bioluminescent fusion protein having at least 95% sequence identity to any one of the propeptide-bioluminescent fusion proteins encoded by any one of the nucleotide sequences of SEQ ID NO: 139-330, or
encoding a propeptide-bioluminescent fusion protein having at least 95% sequence identity to any one of the propeptide-bioluminescent fusion protein sequences of SEQ ID NO: 331-395,

wherein the propeptide-bioluminescent fusion protein having at least 95% sequence identity retains the ability to be cleaved to a bioluminescent protein and an active mature peptide.

30. The nucleic acid construct of claim 1 wherein the cell is of the same species as that from which the propeptide was derived.

31. A vector comprising the nucleic acid construct of claim 1.