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                            Funding & Awards
 FY 2010 Forensic DNA Unit Efficiency Improvement Program
Award Information
Awardee
              City of Oakland
Award #
              2010-DN-BX-K182
Funding Category
              Competitive
Location
                    Oakland, CA
Awardee County
              Alameda
Congressional District
              13
Status
              Closed
Funding First Awarded
              2010
Total funding (to date)
              $114,600
Original Solicitation
              NIJ FY 10 Forensic DNA Unit Efficiency Improvement Program
Description of original award (Fiscal Year 2010, $114,600)
A new application of a known process in the biotechnology arena, can be modified to meet the laboratory's needs and still maintain the high quality DNA typing results. This application has been described for the differential digestion process under the title "DNA Preparation from Sexual Assault Cases by Selective Degradation of Contaminating DNA from the Victim" by Garvin et al.  It involves the use of DNase I for the complete degradation of non-sperm cell DNA without disrupting the sperm heads.
We propose utilizing a modification of the DNase I procedure by incorporating automation with the use of the laboratory's current robotic liquid handlers and high volume format sample preparation devises.  All experiments will utilize currently recognized DNA typing procedures (Quantifiler Duo, Identifiler, 3130 Genetic Analyzer, and GeneMapper ID software). ca/ncf



  
Grant-Funded Publications
        
                      Automating the Differential Digestion Method in the Analysis of Sexual Assault Cases Using Selective Degradation

  
Date Created: August 23, 2010
    
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