NISTIR 7269 

Description and Results of the 2003 NIST/NOAA Interlaboratory 

Comparison Exercise Program for Organic Contaminants in 

Marine Mammal Tissues 

John R. Kucklick 

Rebecca S. Pugh 

Paul R. Becker 

Michele M. Schantz 

Stephen A. Wise 

Teresa K. Rowles 

 
NISTIR 7269 

Description and Results of the 2003 NIST/NOAA Interlaboratory 

Comparison Exercise Program for Organic Contaminants in 

Marine Mammal Tissues 

John R. Kucklick 

Rebecca S. Pugh 

Paul R. Becker 

National Institute of Standards and Technology 

Chemical Science and Technology Laboratory 

Analytical Chemistry Division 

Charleston Laboratory 

Michele M. Schantz 

Stephen A. Wise 

National Institute of Standards and Technology 

Chemical Science and Technology Laboratory 

Analytical Chemistry Division 

Teresa K. Rowles 

National Oceanic and Atmospheric Administration 

National Marine Fisheries Service 

November 2005

U.S. Department of Commerce 

Carlos M. Gutierrez, Secretary 

National Institute of Standards and Technology 

William A. Jeffrey, Director
ii

TABLE OF CONTENTS 

LIST OF APPENDICES .............................................................................................................iii

LIST OF TABLES ....................................................................................................................... iv

ABSTRACT.................................................................................................................................. vi

INTRODUCTION......................................................................................................................... 1

MATERIALS USED IN THE 2003 EXERCISE ....................................................................... 1

EVALUATION OF THE EXERCISE RESULTS..................................................................... 2

RESULTS AND DISCUSSION ................................................................................................... 6

CONCLUSIONS ........................................................................................................................... 7

LITERATURE CITED ................................................................................................................ 8
iii

LIST OF APPENDICES 

A. Tabular results of PCB congener, pesticide, and lipid data reported by all 

laboratories ..........................................................................................................................A1-A52 

B. Graphical results of PCB congener and lipid data reported by all laboratories. The 

Z-scores for Homogenate VI represent 25% of the assigned value so that z = +1 is 

the assigned value plus 25 %, z = -1 is the assigned value minus 25 % and so forth. 

Error bars are ± 1 standard deviation. ..............................................................................B1-B30 

C. Graphical results of pesticide and lipid data reported by all laboratories. The Zscores for Homogenate VI represent 25% of the assigned value so that z = +1 is the 

assigned value plus 25 %, z = -1 is the assigned value minus 25 % and so forth. 

Error bars are ± 1 standard deviation ...............................................................................C1-C19 

D. Tabular summary of methods used for analysis by each laboratory..............................D1-D11 

E. Additional analyte data and notes reported by individual laboratories.........................E1-E10 
iv

LIST OF TABLES 

Table 1: Laboratories Participating in the NIST/NOAA Interlaboratory 

Comparison Exercise Program for Organic Contaminants .......................................................3 

Table 2: Target Analytes for the NIST/NOAA Interlaboratory Comparison 

Exercise Program for Organic Contaminants in Marine Mammal Tissues ..............................4 

Table 3: PCB Congeners in Homogenate VI (ng/g wet mass) Reported by the 

Participants the NIST/NOAA Interlaboratory Comparison Exercise 

Program for Organic Contaminants in Marine Mammal Tissues..............................................10 

Table 4: Organochlorine Pesticides in Homogenate VI (ng/g wet mass) 

Reported by the Participants in the NIST/NOAA Interlaboratory 

Comparison Exercise Program for Organic Contaminants in Marine 

Mammal Tissues...............................................................................................................................11 

Table 5: PCB Congeners in SRM 1945 (ng/g wet mass) Reported by the 

Participants in the NIST/NOAA Interlaboratory Comparison Exercise 

Program for Organic Contaminants in Marine Mammal Tissues..............................................12 

Table 6: Organochlorine Pesticides in SRM 1945 (ng/g wet mass) Reported 

by the Participants in the NIST/NOAA Interlaboratory Comparison Exercise 

Program for Organic Contaminants in Marine Mammal Tissues..............................................13 

Table 7: Table 8: Values (ng/g wet mass) for Polybrominated Diphenyl 

Ether (BDE) Congeners in the Exercise Materials Compared to Published 

Values on SRM 1945. ......................................................................................................................14 

Table 8: Results from the Analysis of SRM 1945 for Fatty Acids..............................................15 

Table 9: Results from the Analysis of Homogenate VI for Fatty Acids.....................................16 
v

DISCLAIMER 

Certain commercial equipment or instruments are identified in this report to specify adequately 

the experimental procedures. Such identification does not imply recommendations or 

endorsement by the National Institute of Standards and Technology nor does it imply that the 

equipment or instruments are the best available for the purpose. 
vi

ABSTRACT 

The National Institute of Standards and Technology (NIST), in support of the National Oceanic 

and Atmospheric Administration’s Marine Mammal Health and Stranding Response Program 

(NOAA/MMHSRP), conducts annual interlaboratory comparison exercises for the determination 

of chlorinated pesticides, polychlorinated biphenyl congeners (PCBs), and trace elements in 

marine mammal tissues. These exercises provide one mechanism for laboratories to evaluate 

their measurement quality and comparability for these constituents in marine mammal tissues. In 

the 2003 exercise, 24 laboratories participated in determining the concentrations of selected 

PCBs and organochlorine pesticides in a homogenized blubber control material “Marine 

Mammal Quality Assurance Exercise Homogenate VI” (Homogenate VI) and Standard 

Reference Material (SRM) 1945 Organics in Whale Blubber. This report includes the results 

reported by the participating laboratories, combined consensus data results, and summary 

statistics for each analyte in the samples. The numerical indices used to assess laboratory 

performance are also discussed. 
1

INTRODUCTION 

Laboratories measuring organic contaminants in the marine environment must assess the 

accuracy and precision of their measurements. Quality control of measurements made on marine 

environmental samples is vital to the accurate assessment of marine pollution and its effects on 

wildlife and human health. NIST aims to improve the quality of analytical measurements of 

organic contaminants in marine and environmental matrices by developing improved analytical 

methods, producing NIST Standard Reference Materials (SRMs) and other control materials, and 

conducting annual interlaboratory comparison exercises. 

Through the NIST National Marine Analytical Quality Assurance Program and with support 

from the NOAA Marine Mammal Health and Stranding Response Program (MMHSRP), NIST 

conducts interlaboratory comparison activities to include analyses of marine mammal tissues. 

The 2003 NIST/NOAA Interlaboratory Comparison Exercise Program for Organic Contaminants 

in Marine Mammal Tissues was modeled after previous exercises (Schantz et al., 1996; Schantz 

et al., 2002; Kucklick et al., 2002). The current exercise was designed to help laboratories assess 

data comparability and quality relative to other groups providing measurements of 

organochlorine contaminants in marine mammal tissues and to link these important 

measurements to a national metrology laboratory. The results of the exercises presented in this 

report should be useful for both assessing current methodology and reducing the variability of 

contaminant data reported on marine mammals. Future exercises will allow for the assessment 

of analytical data quality over time. This report summarizes the 2003 organic contaminant 

exercise including methods used for analysis, data reported by the laboratories on the 

intercomparison materials, and numerical indices used to assess laboratory performance. A 

report describing the 2003 trace element results of this exercise is in preparation. 

MATERIALS USED IN THE 2003 EXERCISE 

The 2003 NIST/NOAA Interlaboratory Comparison Exercise for Organochlorines in Marine 

Mammal Tissues (2003 MMQA) used two materials sent out to 30 laboratories. Twenty-four 

laboratories submitted data for this exercise (Table 1). Participants were asked to make three 

measurements each on two materials: SRM 1945 “Organics in Whale Blubber” and MMQA-VI 

(Homogenate VI), the “unknown.” The unknown material for the 2003 Organic Intercomparison 

Exercise was fat from two polar bears (Ursus maritimus). Four approximately 150 g samples, 2 

samples from each animal, were shipped to the NMMTB as extra material. Both animals were 

adult males and were collected from Barrow, Alaska. The tissues were homogenized and 

blended (Zeisler et al., 1983) on February 7, 2002 and partitioned into sixty-three 8 g to 10 g 

samples to be used as the Unknown QA Material VI. At NIST-Charleston, the blubber tissue 

was stored in a liquid nitrogen (LN2) vapor phase freezer at –150 °C until prepared for this 

exercise. One bottle of this material along with one bottle of SRM 1945 were sent either on dry 

ice or using a liquid nitrogen cooled biological dry shipper via overnight express to each 

participating laboratory. 

Exercise Requirements and Target Analytes 

A suite of analytes was chosen for the exercise based on those used in prior exercises (Schantz et 

al., 1996; Schantz et al., 2002; Kucklick et al., 2002) and several additional analytes were 
2

included to broaden this list (Table 2). In addition the compounds listed in Table 2, participants 

were requested to provide, if possible, values for brominated diphenyl ether congeners, coplanar 

PCB congeners, total toxaphene and toxaphene congeners, chlorinated dioxins and furans and 

fatty acids. Laboratories were requested to make triplicate measurements of these compounds in 

each of the materials and report their data using a data template provided by NIST. Results from 

the exercise were discussed during a workshop held in conjunction with the 2003 Society of 

Marine Mammology Biennial meeting held in Greensboro, North Carolina on December 16, 

2003. 

EVALUATION OF THE EXERCISE RESULTS 

Determination of Laboratory Means for Compounds in Table 3 

Each laboratory reported the results of their analyses (Sample 1, Sample 2, and Sample 3) and 

the mean for each laboratory was calculated. Non-numerical results were reported as “NA” (not 

available). None of the target analytes were below the limit of detection for the participating 

laboratories (Tables 4 - 7 and Appendix A). 

Establishment of Consensus Values 

The following guidelines were used by NIST for establishing the “consensus values” for the 

exercise. The consensus values for Homogenate VI were the mean of all the reported laboratory 

means for a compound after the data were first screened for the presence of outliers by 

examining box plots. Individual means for the majority of data were log normally distributed, 

hence the geometric mean was used. Outliers were not included in the mean. Generally, most or 

all the values were included in the determination of the consensus values (outliers are shown in 

bold in Tables 4 and 5). The target values for the SRM were the certified values, reference 

values, or the consensus value determined from data pooled from prior exercises where SRM 

1945 was also analyzed (Tables 6 and 7). 

Reported Results 

Laboratories were assigned a numerical identification code or (alphabetical code for fatty acids) 

based on the order in which data were received with the exception of NIST Charleston, which 

was Laboratory 1 and NIST Gaithersburg, which was Laboratory 2. The same code was used for 

both materials. The results from the analysis of Homogenate VI and SRM 1945 are summarized 

in Tables 4 through 7. Appendix A shows the tabulated results from the individual laboratories 

for both materials and the results are shown graphically in Appendix B. Appendix C gives the 

methods used for analysis by each laboratory and Appendix D shows data for additional 

analytes. 
3

Table 1: Laboratories Participating in the 2003 NIST/NOAA Interlaboratory Comparison 

Exercise Program for Organic Contaminants. 

Mississippi State Chemical Lab 

Mississippi State 

USA 

Geochemical and Environmental Research Group 

Texas A&M University 

USA 

213 National Food Safety and Toxicology Ctr 

Michigan State University 

USA 

Fisheries and Oceans Canada 

Institute of Ocean Sciences 

Sidney, B.C. 

Canada 

Environmental Research Institute 

University of Connecticut 

USA 

University of Pennsylvania 

Toxicology Laboratory 

USA 

National Laboratory for Environmental Testing 

National Water Research Institute 

Environment Canada 

Canada 

Skidaway Institute of Oceanography 

Savannah, Georgia 

USA 

Wadsworth Center, New York State Dept of Health 

Dept of Environmental Health and Toxicology, SUNY 

at Albany 

USA 

Center for Marine Environmental studies, 

Ehime University 

Japan 

Oklahoma Disease Diagnostic Laboratory and 

Veterinary Pathobiology Laboratory 

Oklahoma State University 

USA 

University of Barcelona 

Barcelona 

Spain 

NOAA/National Marine Fisheries Service 

Highlands, New Jersey 

USA 

NOAA/National Marine Fisheries Service 

Northwest Fisheries Science Center 

Seattle, Washington 

USA 

Laboratory of Environmental Toxicology 

The Norwegian School of Veterinary Science 

OSLO 

Norway 

NIST 

Hollings Marine Laboratory 

Charleston, South Carolina 

USA

NOAA/National Ocean Service 

Center for Coastal Environmental Health and 

Biomolecular Research 

Charleston, South Carolina 

USA 

NIST 

Gaithersburg, Maryland 

USA

Toxicological Center 

University of Antwerp (UA) 

Wilrijk 

Belgium 

Separtment of I. A. and Environmental Chemistry 

General Organic Chemistry Institute, CSIC 

Madrid 

Spain 

Freshwater Institute 

Fisheries and Oceans Canada 

Winnipeg, Manitoba 

Canada 

Department of Biology 

University of Ottawa 

Canada 

Veterinary Medical Center 

Michigan State University 

USA 

Mote Marine Laboratory 

Sarasota, Florida 

USA 

NOAA 

Auke Bay Laboratory 

Juneau, Alaska 

USA 
4

Table 2: Target Analytes for the NIST/NOAA Interlaboratory Comparison Exercise Program 

for Organic Contaminants in Marine Mammal Tissues. 

Pesticides PCB Congeners Congener Substitution 

2,4’-DDT 18 2,2’,5-trichlorobiphenyl 

4,4’-DDT 28 2,4,4’-trichlorobiphenyl 

2,4’-DDE 31 2,4’,5-trichlorbiphenyl 

4,4’-DDE 44 2,2’,3,5’-tetrachlorobiphenyl 

2,4’-DDD 49 2,2’,4,5’-tetrachlorobiphenyl 

4,4’-DDD 52 2,2’,5,5’-tetrachlorobiphenyl 

HCB 66 2,3’,4,4’-tetrachlorobiphenyl 

α-HCH 87 2,2’,3,4,5’-tetrachlorobiphenyl 

γ-HCH 95 2,2’,3,5’,6-tetrachlorobiphenyl 

β-HCH 99 2,2’,4,4’,5-tetrachlorobiphenyl 

heptachlor epoxide 101 2,2’,4,5,5’-tetrachlorobiphenyl 

cis-chlordane 105 2,3,3’,4,4’-tetrachlorobiphenyl 

trans-chlordane 118 2,3’,4,4’,5-tetrachlorobiphenyl 

oxychlordane 128 2,2’,3,3’,4,4’-hexachlorbiphenyl 

cis-nonachlor 132 2,2’,3,3’,4,6’-hexachlorbiphenyl 

trans-nonachlor 138 2,2’,3,4,4’,5’-hexachlorbiphenyl 

dieldrin 149 2,2’,3,4’,5’,6-hexachlorobiphenyl 

mirex 151 2,2’,3,5,5’,6-hexachlorbiphenyl 

 153 2,2’,4,4’,5,5’-hexachlorbiphenyl 

 156 2,3,3’,4,4’,5-hexachlorbiphenyl 

 170 2,2’,3,3’,4,4’,5-heptachlorobiphenyl 

 180 2,2’,3,4,4’,5,5’-heptachlorobiphenyl 

 183 2,2’,3,4,4’,5’,6-heptachlorobiphenyl 

 187 2,2’,3,4’,5,5’,6-heptachlorobiphenyl 

 194 2,2’,3,3’,4,4’,5,5’-octachlorobiphenyl 

 195 2,2’,3,3’,4,4’,5,6-octachlorobiphenyl 

 201 2,2’,3,3’,4,5,5’,6’-octachlorobiphenyl 

 206 2,2’,3,3’,4,4’,5,5’,6-nonachlorobiphenyl 

 209 2,2’,3,3’,4,4’,5,5’,6,6’-decachlorobiphenyl 
5

Assignment of z-and p-scores 

Performance Scores: Different programs have different data quality needs. The acceptability of 

the results submitted by a laboratory will be decided by the individual program(s) for which the 

laboratory provides data. Typically, the program will use these exercise results in conjunction 

with the laboratory’s performance in the analysis of certified reference materials and/or control 

materials, and of other quality assurance samples. These exercise results are shown in a number 

of ways in this report to aid in the evaluation of data quality. 

IUPAC guidelines (IUPAC 1993) describe the use of “z-scores” and “p-scores” for assessment 

of accuracy and precision in interlaboratory comparison exercises, such as described in this 

report. These indices assess the difference between the result of the laboratory and the exercise 

assigned value, and can be used, with caution, to compare performance on different analytes and 

on different materials. 

Accuracy Assessment (z-score):

z = bias estimate / performance criterion 

 

or 

 

z = (x - X)/σ

 

where x is the individual laboratory result, X is the “Exercise Assigned Value,” and σ is the 

target value for the standard deviation. As described in the IUPAC guidelines, the choice of σ is 

dependent upon the data quality objective of a particular program. It can be fixed or determined 

by reference to validated methodology (e.g., the calculated σ from the exercise data, see Tables 4 

through 7). The fixed performance criterion is more useful in the comparison of a laboratory’s 

performance on different materials, while the use of the actual variation may be more useful 

within a given exercise, for example, if the determination of a particular analyte is more 

problematic than usual. 

The z-scores calculated using both approaches and applied to each laboratory’s data are given in 

Appendix A. The same criterion was adopted for use in this exercise as was used in the former 

NIST/NOAA/NS&T program, where the target standard deviation was set to 25 % of the 

exercise assigned value. The z-scores for the Homogenatel VI represent 25 % of the assigned 

value so that z = +1 is the assigned value plus 25 %, z = -1 is the assigned value minus 25 % and 

so forth. z-scores are also calculated based on the standard deviation of an analyte in the control 

material such that z = +1 is one “exercise standard deviation” higher than the assigned value and 

z = -1 is one “exercise standard deviation” lower than the assigned value and so forth. From a 

scientific point of view, IUPAC does not recommend the classification of z-scores, but does 

allow for such classification, e.g.,

⎪z⎪ ≤ 2 Satisfactory 

2 ≤ ⎪z⎪ ≤ 3 Questionable 
6

⎪z⎪ ≥ 3 Unsatisfactory 

The tables in Appendix A summarize the results and performance indices including the number 

of analytes that fall within each category for each laboratory. 

Precision Assessment (p-score):

 p = σlab / σtarget ≈ CVlab/ CVtarget

 

where σlab and σtarget are variance estimates for the individual laboratory and the target variance, 

respectively. The CVlab is the coefficient of variance (or ratio of standard deviation to the mean), 

while the CVtarget is a target value chosen by the participants. During the workshop that 

accompanied this exercise, a target CV of 15% was agreed upon, which is the same value used 

by other NIST run exercise programs (Schantz et al., 1996; Schantz et al., 2002; Kucklick et al., 

2002). Note that the precision that p describes is that which occurs within a batch of analyses. 

Between-batch variance is likely larger and was not assessed in this exercise. 

RESULTS AND DISCUSSION 

Summarized results are shown in Tables 4 through 7 for the compounds listed in Table 3 and 

data for brominated diphenyl ether (BDE) congeners and fatty acids are shown in Tables 8 and 9, 

respectively. The concentration of certain organochlorine compounds in Homogenate VI was 

considerably higher than in SRM 1945, especially for PCB 153 and beta-HCH. The relative 

concentrations of organochlorine compounds in the polar bear were very different than SRM 

1945, which is pilot whale blubber, and consistent with observations from other studies of polar 

bears (e.g. Letcher et al., 1998). The consensus value for the sum of PCB congeners in 

Homogenate VI was 3,580 ng/g wet mass versus 1,350 ng/g wet mass in SRM 1945 (sum of the 

certified and reference values, Table 6). Likewise the sum of the organochlorine pesticides in 

Homogenate VI was 2,610 ng/g wet mass versus 1,500 ng/g wet mass in SRM 1945 (sum of the 

reference values, Table 7). PCB 153 was present in the highest concentration in Homogenate VI 

samples with a consensus value of 1610 ng/g (522 ng/g (1 SD)) compared to a certified value of 

213 ng/g ± 19 ng/g in SRM 1945. Beta-HCH was the compound present in the highest 

concentration in Homogenate VI with a concentration of 1100 ng/g (375 ng/g) versus a reference 

value of 8.0 ng/g ± 1.4 ng/g in SRM 1945. The mean “total extractable organics” determined in 

Homogenate VI was 73.1 % (4.3 %) and the average value determined by the participants in 

SRM 1945 was 73.4 % (7.3 %) relative to the certified value of 74.3 % ± 0.45 % (Table 7). 

The relative scatter among the laboratories appeared similar for many of the compounds with 

some exceptions (Appendix B). As in previous years, this was especially noticeable for PCB 

congener 201 for both the control material and the SRM. This may be a result of the two 

nomenclature systems used for this PCB congener (Guitart et al., 1993). High biases (most 

values exceeding the certified value) were observed among the values determined in the SRM 

relative to the certified value for the following compounds: PCB 87, PCB 99, PCB 180, PCB 

194, PCB 206, PCB 209, and cis-nonachlor. Low biases (most values below the certified or 
7

reference value) were observed for PCB 18, PCB 28, PCB 49, PCB 52, PCB 105, PCB 149, PCB 

195, 4,4’-DDT, 4,4’-DDD, HCB, and trans-nonachlor. 

The data reported on the BDE congeners are given in Table 8. Five laboratories submitted data 

for the BDE congeners for SRM 1945 and four submitted data for Homogenate VI. Data for 19 

BDE congeners were reported along with data for brominated biphenyl 153. A consensus mean 

was derived using the geometric mean of the reported values after screening for outliers using 

box plots. 

The relative proportions and the concentrations of BDE congeners differed between the two 

materials. The concentrations of BDE congeners were at least ten-fold less in Homogenate VI 

than in SRM 1945. The lower concentrations in Homogenate VI are not unexpected based on 

other published BDE measurements in Arctic marine mammals (e.g., Ikonomou et al., 2002). 

Based on the consensus means, BDE 47 comprised 44 % and 73 % of the total BDEs (sum of 

congeners 47, 99, 100, 153, and 154) in SRM 1945 and Homogenate VI, respectively. 

Table 8 also contains literature values for the concentrations of BDE congeners 47, 99, 100, 153, 

and 154 in SRM 1945. In general, consensus values agreed with literature values for SRM 1945 

within a factor of two; however the literature values were all greater than the consensus values 

for unknown reasons. 

Data for a large number (≈ 100) of individual fatty acids were reported by four laboratories 

(Tables 9 and 10). However, only the fatty acids listed in Table 9 and 10 were requested; 

additional fatty acids are listed in Appendix D. A mean value was calculated for laboratories A 

through C as these results are in mg/g wet mass. Laboratory D’s data were not included in this 

as it appeared their data were in units of fraction (%) of fatty acid to the total fatty acids. 

Laboratories E and F’s data are in fraction (%) of fatty acid to the total fatty acids. 

A number of laboratories reported results for analytes in addition to the target compounds 

(Appendix D). Additional analytes that were reported included other PCB congeners (Labs 1, 2, 

4, 6, 7, 8, 19, 22), coplanar PCBs (Labs 5, 6, 8, 13), and other organochlorine pesticides (Labs 1, 

2, 4, 6, 14). 

The participants used a variety of different methods to analyze the materials in this exercise 

(Appendix E). Nine laboratories used Soxhlet extraction to extract the materials, eight used 

pressurized fluid extraction, and the remaining laboratories used other techniques including 

maceration with solvent and column elution. Of the laboratories that responded, eight 

laboratories performed a pre-separation on the sample extracts prior to gas chromatographic 

(GC) analysis (i.e., fractionation), while nine laboratories did not. About half of the laboratories 

used GC/electron capture detection (ECD) to quantify the compounds and the other half used 

GC/mass spectrometry detection (MS) or a combination of GC/ECD and GC/MS. Eight 

laboratories performed a preseparation prior to analysis by GC/MS. Other details are 

summarized in Appendix E. 
8

CONCLUSIONS 

Twenty-four laboratories submitted data for the two exercise materials on a wide variety of 

organic constituents including PCBs, pesticides, fatty acids, and BDE congeners. The increasing 

participation in the exercise indicates the value of the exercise to those labs submitting data. A 

new exercise will be conducted in late 2004 or early 2005 using SRM 1945 and a new unknown 

material. The exercise coordinators plan to vary the type of control material used to include 

other species of interest such as a delphinid. NIST is somewhat constrained on the choice of the 

material as a fairly large quantity (≈ 1 kg) is needed, and this amount of material is not available 

on a routine basis. SRM 1945 will continue to be the SRM used in this exercise, because it is the 

only marine mammal tissue available with certified and reference values for organochlorine 

compounds. The core suite of analytes (Table 3) will remain the same, however data from 

BDEs, toxaphene and fatty acids will be requested. 

LITERATURE CITED 

Guitart, R., Puig, P., and Gomez-Catalan, J. Requirement for a Standardized Nomenclature 

Criterion for PCBs: Computer-Assisted Assignment of Correct Congener Denomination and 

Numbering. Chemosphere 27:1451-1459 (1993). 

Ikonomou M.G., Rayne S., Addison R.F. Exponential Increases of the Brominated Flame 

Retardants, Polybrominated Diphenyl Ethers, in the Canadian Arctic from 1981 to 2000. 

Environ. Sci. Technol. 36:1886-1892 (2002). 

IUPAC. The International Harmonized Protocol for the Proficiency Testing of (Chemical) 

Analytical Laboratories; Pure & Appl. Chem. 65:123-2144 (1993). 

Kucklick, J.R., Christopher, S.J., Becker, P.R., Pugh, R.S., Porter, B.J., Schantz, M.M., Mackey, 

E.A., Wise, S.A., Rowles, T.K. Description and Results of the 2000 NIST/NOAA 

Interlaboratory Comparison Exercise Program for Organic Contaminants and Trace Elements 

in Marine Mammal Tissues. National Institute of Standards and Technology, NISTIR 6849 

(2002). 

Kucklick J.R., Tuerk K.J.S., Vander Pol S.S, Schantz M.M., Wise SA. Polybrominated diphenyl 

ether congeners and toxaphene in selected marine standard reference materials. Anal. 

Bioanal. Chem. 378:1147-1151 (2004). 

Letcher RJ, Norstrom RJ, Muir DCG. Biotransformation versus bioaccumulation: Sources of 

methyl sulfone PCB and 4,4'-DDE metabolites in the polar bear food chain. Environ. Sci. 

Technol. 32:1656-1661 (1998). 

Schantz, M.M., Wise, S.A., Segstro, M., Muir, D.C.G., and Becker, P.R. Interlaboratory 

Comparison Study for PCB Congeners and Chlorinated Pesticides in Beluga Whale Blubber. 

Chemosphere 33:1369-1390 (1996). 
9

Schantz, M.M., Kucklick, J.R., Parris, R.M., and Wise, S.A. NIST Intercomparison Exercise 

Program for Organic Contaminants in the Marine Environment. Description and Results of 

2000 Organic Intercomparison Exercise. National Institute of Standards and Technology 

NISTIR 6837 (2002). 

Zeisler, R., Langland, J.K., and Harrison, J.K. Cryogenic Homogenization Procedure for 

Biological Tissues. Anal. Chem. 60: 2760-2765 (1983). 

Zhu L.Y., Hites R.A.. Determination of Polybrominated Diphenyl Ethers in Environmental 

Standard Reference Materials. Anal. Chem. 75(23):6696-6700 (2003). 
Table 3: Mean polychlorinated biphenyl congener concentrations in Homogenate VI (ng/g wet mass) reported by each laboratory. The values in bold are outliers and were not used to determine the geometric consensus mean. N

Laboratory Reported C

Compound 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Mean 1 SD n 18 <2 <5 <8.20 <1.55 NA 6.64 DL DL NA <9.69 6.83 NA 0.204 NA NA NA NA <0.5 NA NA NA NA NA 2.10 3.8 3 28 <2 <6 <11.3 <1.56 NA 3.49 8.50 0.280 NA <9.69 11.5 NA 1.01 NA 0.282 <0.11 13.1 0.433 NA 1.79 NA NA 3.33 1.98 4.9 10 31 w/28 <5 NA <1.17 NA DL DL 0.143 NA NA NA NA 0.273 NA 1.93 <0.16 NA <0.5 NA NA NA NA NA 0.422 1.0 3 44 <2 <5 <10.5 <1.55 NA 6.91 5.64 0.235 1.09 26.5 7.33 NA <0.100 NA 2.10 NA NA <0.5 34.1 NA NA NA 7.50 4.66 12 9 

49 <2 22.1 13.3 <1.55 NA 6.93 9.13 0.712 ND NA NA NA 0.321 NA 2.29 NA NA 0.697 45.9 NA NA NA 3.62 4.03 14 10 

52 <2 8.29 <10.8 1.81 NA 10.6 10.2 1.73 4.19 22.3 61.7 NA 0.293 NA 2.89 4.78 4.65 1.63 105 7.82 NA NA 1.92 5.54 28 16 66/95 below below below below NA 8.14 13.8 NA below NA 3.03 NA below NA 2.88 <0.13 NA below NA 1.97 NA NA NA 4.54 5.0 5 

87 <2 <10 <9.4 7.82 NA see notes DL 6.09 NA <=105 NA NA <0.1 NA 6.92 <0.10 NA 5.67 30.7 NA NA NA 4.24 7.90 10 6 

99 347 312 312 319 NA 372 466 397 262 NA NA NA 359 NA 335 377 NA 342 74.9 NA NA NA 435 316 92 14 

101 (+90) <10 4.96 17.9 4.86 NA 12.5 27.9 4.29 176 11.5 10.9 NA 1.51 NA 4.65 21.5 16.6 4.91 113 6.76 NA NA 12.8 11.8 46 17 

105 16.3 NA <15.4 16.7 25.0 18.6 35.5 15.7 14.6 32.6 12.5 NA 19.5 NA 12.1 21.5 18.6 15.9 42.3 8.05 NA NA 22.9 18.9 8.9 17 

118 60.0 73.4 47.7 60.4 68.9 69.8 101 57.9 61.9 50.6 41.0 NA 77.7 NA 45.3 58.5 139 57.9 135 38.9 NA NA 49.7 63.9 28 19 128 9.29 11.2 <55 11.9 NA 12.8 16.8 11.3 11.0 16.0 8.80 NA 12.9 NA 11.6 13.8 13.9 11.3 NA NA NA NA 9.65 12.0 2.3 15 132 w/153 <8 NA NA NA see notes DL NA NA NA NA NA <1.0 NA NA NA NA NA NA NA NA NA NA NA NA 0 138 (+163+164) 213 see notes 85.7 286 214 322 540 346 237 276 111 NA 205 NA 173 292 241 235 412 250 NA NA NA 240 107 17 149 2.57 <8 <11.3 2.59 NA 4.55 261 2.47 NA NA NA NA 3.91 NA 2.64 1.72 1.93 2.58 196 NA NA NA NA 5.97 92 11 151 <2 <8 <8.90 <1.56 NA DL ND 0.820 0.970 NA NA NA 0.215 NA NA 0.557 3.33 0.927 58.4 NA NA NA 7.65 1.84 20 8 

153 2055 1991 1907 1887 2317 1797 3944 NA 1303 1997 936 NA 2029 NA 1007 2250 1345 1808 548 2173 NA NA NA 1695 741 17 

156 58.7 65.5 48.4 63.9 76.8 78.9 79.2 60.1 70.7 NA NA NA 79.0 NA NA 76.3 63.7 69.1 30.4 49.8 NA NA 27.9 59.9 16 16 170 (+190) 440 345 172 410 526 505 479 373 363 278 231 NA 347 NA 264 427 249 364 72.5 202 NA NA 340 309 119 19 180 670 see notes 561 734 802 843 1484 869 596 606 457 NA 943 NA 440 958 492 752 318 791 NA NA 723 684 261 18 

183 20.8 22.0 <18.9 23.4 NA 33.0 50.3 26.5 31.8 NA NA NA 21.2 NA 17.7 22.2 23.6 23.4 87.0 12.8 NA NA 28.0 26.4 18 15 

187 7.40 <10 <14.6 8.75 NA 14.6 8.95 9.49 NA 13.5 5.70 NA 9.60 NA 7.84 5.75 19.6 8.87 295 NA NA NA 21.4 12.7 76 14 194 167 142 124 156 NA 227 301 170 142 228 NA NA 167 NA 103 219 120 163 198 136 NA NA 120 163 51 17 195 3.27 <15 <7.3 2.59 NA 6.29 7.29 3.37 3.41 4.65 8.93 NA 3.94 NA 2.42 NA 4.53 3.51 34.2 NA NA NA NA 4.91 8.4 13 

201 <2 NA <5.6 NA NA 13.3 86.9 4.92 5.39 <2.40 NA NA 5.76 NA 5.04 NA 7.03 NA 81.0 NA NA NA NA 12.3 36 8 

206 37.2 35.1 26.2 38.7 NA see notes 68.8 36.2 34.3 45.4 25.8 NA 34.2 NA 26.0 35.9 31.6 NA NA NA NA NA 22.5 34.2 11 14 

209 12.3 12.3 8.97 12.9 NA 44.9 27.8 13.3 11.8 15.6 16.4 NA 13.0 NA 9.92 12.1 11.9 NA NA NA NA NA 10.3 14.1 9.2 15 

66 3.12 <10 <11.7 <1.55 NA NA NA 1.06 1.97 <9.69 NA NA 0.297 NA NA NA NA 0.863 79.3 NA NA NA 4.49 2.49 29 7 95 <2 <10 <9.70 0.985 NA NA NA 0.887 1.32 NA NA NA <0.125 NA NA NA 87.6 1.47 53.7 NA NA NA 0.96 3.59 35 7 

NA=Not Available below=see below for results prestented individually.DL=Below Detection Limit
Table 4: Mean organochlorine pesticide and lipid concentrations in Homogenate VI determined by each laboratory (ng/g wet mass and percent (mass fraction), respectively). Notes are given in App

The values in bold are outliers and were not used to determine the geometric consensus mean.

Laboratory Reported 

Compound 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Mean 1 SD n 2,4’-DDT <2 <5 39.5 1.80 <2.2 18.9 5.56 2.92 NA <2.60 2.13 <5 <2.0 NA NA <0.15 34.0 <1.0 NA NA NA NA 2.17 6.56 16 8 4,4’-DDT 4.09 5.40 <137 7.02 NA 16.0 8.35 6.79 6.13 <=516 9.90 <5 10.6 75.4 15.7 <0.83 12.7 12.0 6.83 16.6 NA NA 6.29 10.1 17 16 2,4’-DDE <2 <5 <7.40 <1.54 NA 3.20 ND 0.144 NA 23.1 5.70 <5 <2.0 NA NA NA 5.64 NA NA NA NA NA 6.45 5.70 31 7 4,4’-DDE 71.0 59.9 85.2 83.8 87.9 82.2 159 86.6 88.9 7.60 42.6 67.3 96.0 56.6 77.7 66.5 107 96.4 45.8 55.2 NA NA 78.7 68.3 29 21 

2,4’-DDD 1.58 <5 <15.3 <1.54 <3.5 5.93 ND 0.249 NA 10.9 <1 <5 <2.0 NA NA <0.14 NA <1.0 NA NA NA NA 2.2 2.23 4.3 5 4,4’-DDD 2.51 4.38 <44.5 2.63 <3.5 see notes 3.62 2.13 3.89 3.87 1.15 <5 3.36 13.6 7.65 1.23 4.34 5.86 1.90 10.5 NA NA 5.65 4.75 73 18 

HCB 251 277 197 270 321 182 432 295 218 324 180 NA 174 NA 245 244 179 248 136 NA NA NA 358 242 75 18 alpha-HCH 63.5 67.2 54.8 72.4 NA see notes 109 120 70.2 9.17 44.3 <5 60.9 44.6 79.0 52.4 62.5 57.6 48.0 NA NA NA 84.2 58.3 25 17 

beta-HCH 1738 869 1197 1190 NA 1092 1964 2918 964 1150 688 1065 1081 630 NA 980 675 1650 1283 NA NA NA 1465 1164 552 18 

gamma-HCH <2 <15 <1 2.65 NA 4.67 2.86 1.81 2.34 2.17 2.53 <5 2.84 NA 3.14 1.97 5.60 1.44 NA NA NA NA 4.46 2.75 1.2 13 Heptachlor Epoxide 139 77.5 101 99.9 NA 81.4 143 146.3 71.9 107 49.4 92.6 118 66.9 NA <0.074 NA NA NA NA NA NA 103 95.5 29 14 Cis-Chlordane <2 25.6 <10.6 <1.54 NA DL ND 96.3 1.56 22.9 8.77 <5 <2.0 10.1 NA 81.3 NA NA DL NA NA NA 89.1 22.1 40 8 Trans-Chlordane 27.2 <5 <41.4 2.27 NA 10.2 6.24 9.30 43.7 142 1.43 NA <2.0 NA NA 3.41 NA NA DL NA NA NA 9.61 10.1 43 10 

Oxychlordane 631 402 NA 475 NA 443 656 720 450 706 308 429 467 642 NA 305 NA 508 1172 NA NA NA 750 534 215 16 Cis-Nonachlor 4.03 6.09 <7.20 6.31 NA see notes 7.65 6.98 6.84 3.43 4.03 <5 7.56 NA NA 5.19 NA NA 3.93 NA NA NA 5.26 5.42 1.5 12 Trans-Nonachlor 155 156 179 182 NA 150 318 214 168 192 97.2 162 193 NA NA 160 NA NA 88.9 NA NA NA 166 165 52 15 

Dieldrin 267 151 220 227 NA see notes 291 177 223 275 122 209 198 65.0 219 NA NA NA NA NA NA NA 280 196 64 14 

Mirex 6.81 15.0 <10.4 11.8 NA 15.5 155 8.79 11.2 66.1 8.23 <5 14.4 NA 19.8 10.3 NA NA NA NA NA NA 6.44 15.5 42 13 

Lipid 68.8 72.6 71.0 71.1 57.9 74.9 71.6 72.2 67.2 73.9 74.5 72.2 66.8 NA 72.5 75.8 72.6 82.3 78.7 80.0 NA 78.2 66.9 72.3 5.3 21 

NA=Not AvailableDL=Below Detection Limit
Table 5: Mean polychlorinated biphenyl congener concentrations in SRM 1945 determined by each laboratory (ng/g we mass). Notes are given in Appendix E.

Laboratory Geometric Refere

Congener 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Mean 1 SD n Valu18 4.53 <5 <8.20 3.09 NA 9.17 3.09 1.89 NA <9.69 2.23 NA 3.82 NA 2.69 NA NA 2.29 NA NA NA NA NA NA 2.2 9 4.428 16.3 14.5 12.4 9.85 NA 23.0 15.1 9.12 NA 8.95 6.03 NA 10.3 NA 9.57 9.84 9.66 11.6 NA 24.1 NA NA 15.2 12.1 5.0 16 14.131 w/28 3.60 nd 4.30 NA 15.1 NA 2.48 NA NA NA NA 4.83 NA 3.92 <0.16 NA 2.85 NA NA NA NA 2.19 4.02 4.2 8 3.144 10.7 14.1 <10.5 11.7 NA 20.5 11.3 12.2 9.42 13.7 6.23 NA 11.3 NA 10.2 NA NA 12.0 NA NA NA NA 13.8 11.7 3.3 13 12.49 17.5 18.9 14.1 16.9 NA 13.9 35.8 16.3 14.5 NA NA NA 15.5 NA 13.9 NA NA 16.7 9.22 NA NA NA 20.9 16.4 6.3 13 20.52 36.8 44.6 35.1 38.1 NA 44.2 55.8 37.3 30.6 46.9 96.8 NA 41.7 NA 26.1 36.6 28.3 38.1 25.4 72.2 NA NA 31.9 40.1 18 18 43.66/95* below below below below NA 70.5 87.0 NA below NA 21.2 NA below NA 37.4 21.1 NA below NA 46.5 NA NA NA 41.0 27 6 57.87 21.4 19.6 19.6 25.3 NA see notes 33.8 23.6 NA <=443 NA NA 24.4 NA 24.3 16.8 NA 23.8 6.91 NA NA NA 17.5 20.2 6.4 12 16.99 57.6 51.9 48.2 54.8 NA 66.8 68.0 61.3 54.1 NA NA NA 123 NA 94.3 72.2 NA 54.8 204 NA NA NA 48.0 68.8 42 14 45.101 (+90) 80.8 71.4 52.0 77.5 NA 120 95.2 88.5 96.3 59.6 72.5 NA 160 NA 54.3 83.2 54.1 77.5 14.1 80.9 NA NA 42.6 69.5 31 18 65.105 26.9 NA 19.5 27.1 34.9 59.3 29.3 26.3 21.7 26.7 27.1 NA 49.1 NA 18.5 34.3 30.8 26.1 13.3 11.9 NA NA 15.4 25.6 12 18 30.118 80.7 76.5 67.5 83.8 120 101 157 76.9 71.2 98.9 50.0 NA 103 NA 59.3 72.9 88.7 74.1 52.6 47.5 NA NA 56.3 77.3 27 19 74.128 20.4 21.9 <55 24.5 NA 41.8 23.8 21.5 18.3 62.0 18.0 NA 30.5 NA 76.6 29.1 26.4 20.5 NA NA NA NA 24.2 27.7 17 15 23.132 w/153 20.0 NA NA NA see notes 27.4 296 13.9 NA NA NA 2.94 NA 14.6 NA NA 17.8 30.6 7.5 NA NA NA 19.4 94 9 22.138 (+163+164) 137 see notes 59.4 183 134 197 273 214 136 209 94.9 NA 161 NA 112 175 154 134 330 138 NA NA NA 156 65 17 131149 71.5 92.1 71.0 85.2 NA 83.8 137 101 66.9 NA NA NA 93.6 NA 59.8 55.0 72.3 77.2 15.1 52.1 NA NA NA 69.4 27 15 107 151 24.0 27.9 30.2 27.5 NA 29.5 62.4 31.4 21.4 NA NA NA 24.3 NA NA 22.8 34.6 22.6 DL NA NA NA 20.9 27.9 11 13 28.153 244 238 209 243 297 245 342 NA 171 275 134 NA 240 NA 141 233 216 192 2442 179 NA NA NA 252 540 17 213156 10.1 10.2 <11.1 12.6 7.9 19.1 16.5 10.0 9.75 NA NA NA 23.4 NA NA 12.7 25.0 10.7 85.4 3.9 NA NA 6.08 13.0 20 15 10.170 (+190) 47.2 40.6 31.0 50.3 99.4 58.3 73.0 39.2 40.7 32.6 23.2 NA 42.6 NA 34.5 42.9 33.9 37.0 345 20.2 NA NA 22.0 44.2 72 19 40.180 133 see notes 109 144 139 200 226.3 153 118 129 86.8 NA 162 NA 94.8 164 127 130 854 110 NA NA 105 147 173 18 106183 36.6 36.0 31.2 39.6 NA 46.1 81.3 47.5 21.3 NA NA NA 53.6 NA 28.0 37.4 35.2 34.8 27.6 20.8 NA NA 20.1 34.9 15 16 36.187 128 112 94 114 NA 199 118 156 NA 158 82.7 NA 132 NA 81.1 81.9 106 117 13.3 NA NA NA 91.7 99.9 41 16 105 194 59.1 46.3 42.4 60.8 NA 84.3 85.5 60.1 45.6 76.0 NA NA 54.6 NA 46.3 87.3 63.2 53.6 263 42.2 NA NA 29.7 61.5 52 17 39.195 10.6 <15 <7.3 11.6 NA 41.1 17.4 15.2 24.9 14.0 12.8 NA 11.9 NA 5.78 NA 15.1 9.03 5.98 NA NA NA NA 13.1 9.3 13 17.201 11.8 NA 10.2 NA NA 87.9 24.8 88.9 65.9 16.0 NA NA 126 NA 55.0 NA 69.4 NA NA NA NA NA NA 40.5 39 10 16.9206 49.8 43.5 34.3 50.6 NA see notes 79.9 45.8 38.5 68.7 31.4 NA 51.5 NA 34.4 52.3 40.7 NA NA NA NA NA 19.7 43.4 15 14 31.209 17.8 14.2 12.7 18.5 NA 23.0 31.5 17.8 14.2 21.5 15.0 NA 14.6 NA 15.5 18.6 20.1 NA NA NA NA NA 10.3 17.1 5.1 15 10.66 22.5 22.5 17.6 22.3 NA NA NA 23.4 15.5 41.7 NA NA 29.4 NA NA NA NA 20.8 10.7 NA NA NA 23.0 21.5 7.9 11 23.95 34.4 33.8 30.2 39.9 NA NA NA 38.7 24.7 NA NA NA 41.4 NA NA NA 39.5 36.5 10.8 NA NA NA 36.7 31.6 8.9 11 33.NA=Not Available below=see below for results prestented individually.DL=Below Detection Limit
Table 6: Mean organochlorine pesticide and lipid concentrations in SRM 1945 determined by each laboratory (ng/g wet mass and percent (mass fraction), respectively). Notes are given in Appendix 

Laboratory Geometric Reference

Compound 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Mean 1 SD n Value 2,4’-DDT 61.8 94.8 69.1 88.7 38.6 116 148 92.6 NA 138 60.6 <5 107 NA 120 77.5 98.8 96.4 NA NA NA NA 55.1 86.4 30 16 106 4,4’-DDT 210 226 141 257 191 402 415 216 243 <=506 110 138 245 196 309 254 209 297 150 40.4 NA NA 132 198 92 20 245 2,4’-DDE 11.7 12.5 9.40 14.7 NA 26.9 13.3 13.1 NA 14.4 8.97 <5 16.1 NA NA NA 8.5 NA NA NA NA NA 7.97 12.4 5.1 12 12.30 4,4’-DDE 501 465 442 488 543 514 778 530 395 459 348 359 432 306 525 479 462 551 340 247 NA NA 464 446 110 21 445.0 2,4’-DDD 16.8 18.5 <15.3 21.7 25.3 31.0 30.7 15.8 NA 23.7 40.8 <5 17.9 NA NA 30.6 NA 7.20 NA NA NA NA 12.0 20.6 9.2 13 18.1 4,4’-DDD 115 137 64.4 134 112 see notes 198 85.2 104 135 71.2 756 140 134 119 96.1 130 142 126 6.17 NA NA 78.7 108 149 20 133 HCB 26.1 32.4 17.9 27.2 30.7 34.4 44.1 26.3 21.6 32.4 21.1 NA 23.5 NA 28.9 23.9 26.0 28.6 14.8 NA NA NA 35.1 26.7 6.9 18 32.9 alpha-HCH 13.1 16.6 16.9 16.2 NA see notes 20.1 23.0 13.6 16.9 9.27 <5 16.2 10.2 11.3 13.9 15.9 14.2 7.39 NA NA NA 9.34 13.8 4.1 17 16.2 beta-HCH <10 6.2 <59.4 NA NA 18.2 2.23 3.23 1.73 <9.99 2.00 <10 8.29 9.00 NA 2.66 6.28 1.81 4.8 NA NA NA 9.95 4.49 4.7 13 8.0 gamma-HCH 3.6 <10 <1 3.12 NA 17.5 3.48 3.68 2.50 2.6 2.03 <5 3.26 NA 2.19 2.24 3.61 3.37 NA NA NA NA 3.82 3.37 3.9 14 3.3 Heptachlor Epoxide 10.5 <10 9.70 12.4 NA 16.7 15.1 16.9 8.32 12.2 8.20 <5 15.7 10.6 8.53 <0.074 NA NA NA NA NA NA 9.08 11.4 3.2 13 10.8 Cis-Chlordane 44.2 46.7 40.4 56.9 NA 60.5 76.7 106.1 43.9 66.5 31.3 37.9 55.3 43.9 29.8 61.4 NA NA 25.6 NA NA NA 35.6 47.6 20 17 46.9 Trans-Chlordane 10.1 11.0 <41.4 12.9 NA 27.3 20.7 17.3 14.5 50.2 <1 NA 10.2 NA 17.8 13.1 NA NA 6.6 NA NA NA 2.24 13.2 12 13 12.1 Oxychlordane 22.7 20.1 NA 23.0 NA 22.9 24.0 28.0 16.6 22.9 14.4 24.3 21.5 26.5 NA 12.3 NA 22.2 44.2 NA NA NA 23.2 22.2 7.0 16 19.8 Cis-Nonachlor 49.3 46.1 32.5 58.4 NA see notes 54.7 68.7 44.8 42.3 35.1 36.3 46.6 NA NA 45.8 NA NA 25.7 NA NA NA 34.9 43.0 11 14 48.7 Trans-Nonachlor 157 196 170 182 NA 186 297 250 142 215 105 171 186 NA NA 166 NA NA 82.9 NA NA NA 129 168 53 15 231 Dieldrin 53.3 38.5 <61.9 55.4 NA see notes 52.6 39.4 46.7 51.0 29.2 519 43.6 37.6 35.0 NA NA NA NA NA NA NA 41.0 51.9 132 13 37.5 Mirex 29.7 28.1 21.3 38.3 NA 50.6 30.9 29.9 28.4 36.7 20.9 <5 28.5 NA 46.7 35.0 NA NA NA NA NA NA 15.9 30.2 10 14 28.9 Lipid 70.9 74.9 71.0 73.5 59.8 73.6 70.1 71.6 66.1 81.0 73.8 73.4 70.3 NA 71.1 74.2 75.0 78.1 74.6 91.0 NA 90.7 63.7 73.4 7.3 21 74.3 NA=Not AvailableDL=Below Detection Limit
SRM 1945

Laboratory

Compound 2 7 16 18 22 Mean 1 SD n Mean 1SD Mean 1 SD

BDE 15 NA NA NA NA 0.0252 1

BDE 17 DL NA NA NA NA 0

BDE 28 20.2 NA NA 1.243 1.018 3

BDE 71 DL NA NA NA NA 1

BDE 47 44.4 39.2 33.0 35.6 33.5 36.9 4.7 5 46.6 2.0 52.9 3.8

BDE 66 DL NA NA NA NA 1

BDE 85 4.57 NA NA NA NA 1

BDE 99 17.9 17.9 16.4 16.9 16.0 17.0 0.9 5 23.0 1.9 32.0 2.3

BDE 100 13.5 7.17 8.57 8.73 8.92 9.17 2.4 5 11.8 0.9 14.0 0.6

BDE 153 8.47 6.39 7.54 7.42 8.04 7.54 0.78 5 10.1 1.0 22.4 1.5

BDE 154 23.1 11.1 10.8 10.7 12.1 12.9 5.4 5 20.8 3.0 28.2 2.0

BDE 138 DL NA NA NA NA 1

BDE 183 6.16 NA NA 1.55 1.66 3

BDE 190 DL NA NA NA NA 1

BDE 196 NA NA NA NA 0.0939 1

BDE 197 NA NA NA NA 0.422 1

BDE 206 NA NA NA NA DL 1

BDE 207 NA NA NA NA DL 1

BDE 209 NA NA NA NA DL 1

BB 153 NA NA NA 3.18 NA 1

Homogenate VI

Laboratory

Compound 2 7 16 18 22 Mean 1 SD n

BDE 15 NA NA NA NA DL 0

BDE 17 NA NA NA NA NA 0

BDE 28 NA NA NA NA NA 0

BDE 71 NA NA NA NA NA 0

BDE 47 NA 6.10 4.10 4.48 4.38 4.76 0.90 4

BDE 66 NA NA NA NA NA 0

BDE 85 NA NA NA NA NA 0

BDE 99 NA 0.973 0.363 0.413 0.374 0.531 0.30 4

BDE 100 NA 0.294 0.329 0.383 0.263 0.317 0.05 4

BDE 153 NA 0.655 0.703 1.06 0.774 0.799 0.18 4

BDE 154 NA 0.011 <0.15 0.18 0.044 3

BDE 138 NA NA NA NA NA 0

BDE 183 NA NA NA 0.273 NA 1

BDE 190 NA NA NA NA NA 0

BDE 196 NA NA NA NA NA 0

BDE 197 NA NA NA NA NA 0

BDE 206 NA NA NA NA DL 1

BDE 207 NA NA NA NA DL 1

BDE 209 NA NA NA NA 0.834 1

BB 153 NA NA NA 2.98 NA 1

NA=Not Available

DL=Below Detection Limit

Table 7: Values (ng/g wet mass) for polybrominated diphenyl ether (BDE) congeners in the exercise materials compared to 

published values on SRM 1945. BB is bromobiphenyl. 

(2003) (2004)

Consensus

Consensus Zhu and Hites Kucklick et al.
Table 8: Results from the analysis of SRM 1945 for fatty acids. Fatty acids not requested for the exercise 

are given in Appendix E.

Compound Mean 1 SD

A B C D E F A-C only

mg/g wet mg/g wet mg/g wet mg/g wet % of total % of total mg/g wet mg/g wet

C12:0 1.83 1.21 2.91 0.464 0.257 0.421 1.98 0.9

C14:0 35.1 21.7 46.3 4.91 5.11 5.79 34.4 12.3

C15:0 3.42 2.96 4.56 0.511 0.487 0.600 3.65 0.8

C16:0 79.0 33.5 101 10.5 11.8 11.5 71.3 35

C17:0 2.48 1.62 3.47 0.368 0.32 0.37 2.52 0.9

C18:0 14.1 9.6 17.1 1.98 1.92 2.44 13.6 3.8

C20:0 1.29 0.87 1.08 0.257 0.130 NA 1.08 0.2

C16:1(n-7) 64.1 46.6 83.5 8.30 9.19 9.42 64.7 18

C18:1(n-7) 19.8 14.1 27.4 NA 2.74 4.25 20.4 6.7

C18:1(n-9) 152 153 195 17.1 22.2 21.3 167 25

C18:1(n-9) 2.60 2.44 1.18 0.15 NA NA 2.07 0.8

C20:1(n-7) NA NA NA NA 0.35 NA NA NA

C20:1(n-9) 51.9 30.5 68.7 5.47 6.97 8.16 50.4 19

C:20:1(n-11) 2.68 25.6 NA NA 4.49 5.32 14.1 16.2

C22:1(n-9) 7.57 4.79 8.70 0.54 0.77 1.41 7.02 2.0

C22:1(n-11) NA 41.17 NA NA 6.82 7.67 41.2 NA

C24:1(n-9) NA NA 3.05 0.270 0.30 NA 3.05 NA

C18:2(n-6) 8.87 6.21 12.3 1.044 1.18 NA 9.12 3.0

C18:3(n-3) 3.92 6.54 8.08 0.503 0.42 NA 6.18 2.1

C18:3(n-6) 0.211 0.097 0.220 0.044 NA NA 0.18 0.1

C18:4(n-3) 1.84 NA NA NA 0.329 NA 1.84 NA

C20:2(n-6) 2.66 1.50 3.16 NA 0.262 NA 2.44 0.9

C20:3(n-3) 1.81 NA NA 0.228 0.190 NA 1.81 NA

C20:4(n-6) 2.67 NA 3.391 0.607 0.329 NA 3.03 NA

C20:5(n-3) 12.5 12.5 18.1 2.04 1.67 1.62 14.4 3.2

C22:2(n-6) 0.227 0.138 NA 0.072 NA NA 0.183 0.06

C22:5(n-3) 9.27 8.10 14.2 1.33 1.28 1.74 10.5 3.3

C22:6(n-3) 41.0 30.9 60.6 5.51 5.19 3.77 44.2 15

Laboratory
Table 9: Results from the analysis of Homogenate VI for fatty acids. Fatty acids not requested for the exercise 

are given in Appendix E.

Compound Mean 1 SD

A B C D E F A-C only

mg/g wet mg/g wet mg/g wet mg/g wet % of total % of total mg/g wet mg/g wet

C12:0 0.485 0.295 0.767 0.520 0.066 0.061 0.516 0.2

C14:0 18.4 13.6 24.5 3.45 2.63 3.05 18.9 5.5

C15:0 2.24 1.73 3.23 0.482 0.314 0.342 2.40 0.8

C16:0 64.6 37.5 85.4 10.2 9.36 9.47 62.5 24

C17:0 2.47 1.65 3.14 0.275 0.305 0.38 2.42 0.7

C18:0 21.5 15.2 28.5 2.39 3.11 3.87 21.7 6.7

C20:0 0.940 0.497 1.08 0.235 0.09 NA 0.840 0.3

C16:1(n-7) 93.3 70.5 125 9.68 13.3 12.4 96.4 28

C18:1(n-7) 55.7 40.9 80.8 NA 7.86 9.83 59.1 20

C18:1(n-9) 166 180 223 21.8 23.2 24.1 190 29

C18:1(n-9) 20.5 5.99 3.14 0.121 NA NA 9.86 9.3

C20:1(n-7) NA NA NA NA 2.99 NA NA NA

C20:1(n-9) 32.2 21.4 46.6 3.47 4.40 6.11 33.4 13

C:20:1(n-11) 0.688 8.94 NA NA 2.96 2.80 4.81 5.8

C22:1(n-9) 3.525 2.24 4.69 0.586 0.376 0.389 3.48 1.2

C22:1(n-11) NA 5.095 NA NA 0.781 0.823 5.10 NA

C24:1(n-9) NA NA 0.800 0.370 0.068 NA 0.800 NA

C18:2(n-6) 7.60 4.96 11.1 0.904 0.942 NA 7.89 3.1

C18:3(n-3) 1.71 1.938 30.8 0.445 0.104 NA 11.48 17

C18:3(n-6) 0.640 0.353 0.709 0.091 NA NA 0.57 0.2

C18:4(n-3) 1.80 NA NA NA 0.190 NA 1.80 NA

C20:2(n-6) 3.10 1.69 2.77 NA 0.324 NA 2.52 0.7

C20:3(n-3) 0.530 NA NA 0.203 0.039 NA 0.530 NA

C20:4(n-6) 3.87 NA 4.91 0.760 0.45 NA 4.39 NA

C20:5(n-3) 16.7 16.0 24.7 2.23 2.39 2.22 19.1 4.8

C22:2(n-6) 0.130 0.082 NA 0.010 NA NA 0.106 0.03

C22:5(n-3) 48.3 34.1 78.4 0.799 6.86 6.17 53.6 23

C22:6(n-3) 46.2 34.1 68.4 5.53 6.00 3.94 49.6 17

Laboratory
Tabular results of PCB congener, pesticide, fatty acid, and lipid data 

reported by all laboratories

Appendix A
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES La

SAMPLES: HOMOGENATE VI AND SRM 1945

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

2,4’-DDT <2 <2 <2 60.4 63.9 61.1 <2 62 3.0 <5 -- 106 14

4,4’-DDT 4.0 3.9 4.3 209 210 211 4.09 5.3 210 0.4 8.83 2.2 245 15 

2,4’-DDE <2 <2 <2 11.6 11.7 11.8 <2 11.7 0.9 <5 -- 12.3 0.87

4,4’-DDE 69.9 70.7 72.4 503 507 495 71.0 1.8 501 1.2 72.3 8.0 445 37 

2,4’-DDD 1.53 1.55 1.67 17.8 15.7 16.9 1.58 4.8 17 6.3 <5 -- 18.1 2.8

4,4’-DDD 2.51 2.56 2.45 115 113 116 2.51 2.2 115 0.9 3.71 1.6 133 10 

HCB 254 254 246 26.5 26.1 25.9 251 1.8 26.1 1.1 242 36 32.9 1.7 

α-HCH 61.7 69.4 59.4 13.3 12.8 13.3 63.5 8.3 13.1 2.1 65.4 11 16.2 3.4 

β-HCH 1653 1909 1653 <2 <2 <2 1738 8.5 <2 1102 184 8.00 1.4 

γ-HCH <2 <2 <2 3.41 4.78 2.7 <2 3.61 30 <5 -- 3.30 0.81

Heptachlor Epoxide 131 151 136 9.51 11.3 10.6 139 7.5 10.5 8.8 95.5 15 10.8 1.3 

Cis-Chlordane <2 <2 <2 45.2 42.8 44.6 <2 44.2 2.9 22.1 29 46.9 2.8

Trans-Chlordane 26.7 27.0 27.8 9.9 10.0 10.4 27.2 1.9 10.1 2.2 6.02 10 12.1 1.4 

Oxychlordane 589 665 640 21.2 24.9 22.1 631 6.1 22.7 8.5 534 113 19.8 1.9 

Cis-Nonachlor 4.1 3.8 4.2 44.4 55.7 47.8 4.03 5.3 49.3 12 5.25 0.8 48.7 7.6 

Trans-Nonachlor 154 159 151 155 162 155 155 2.5 157 2.4 157 19 231 11 

Dieldrin 265 289 248 52.8 51.2 55.8 267 7.7 53.3 4.4 214 27 37.5 3.9 

Mirex 6.87 6.82 6.74 29.1 29.9 30.1 6.81 1.0 29.7 1.8 11.0 2.5 18.9 2.8 

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) 66.5 69.7 70.2 70.2 71.7 70.9 68.8 2.92 70.9 1.06 6.4 2.08 74.3 0.45 

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES LaSAMPLES: HOMOGENATE VI AND SRM 1945

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 <2 <2 <2 4.7 4.8 4.1 <2 4.53 7.5 <5 -- 4.48 0.88

28 <2 <2 <2 16.7 16.5 15.8 <2 16.3 2.9 <5 -- 14.10 1.4

31 w/28 w/28 w/28 w/28 w/28 w/28 w/28 w/28 <5 -- 3.12 0.69

44 <2 <2 <2 10.6 10.9 10.6 <2 10.7 1.8 <5 -- 12.2 1.4

49 <2 <2 <2 17.4 17.8 17.3 <2 17.5 1.7 <5 -- 20.8 2.852 <2 <2 <2 36.4 37.3 36.8 <2 36.8 1.3 3.78 3.0 43.6 2.566/95* below below below below below below below below 3.44 2.7 57.4 2.5

87 <2 <2 <2 20.7 21.8 21.6 <2 21.4 2.8 6.02 1.2 16.7 1.4

99 344 345 351 56.7 57.7 58.5 347 1.1 57.6 1.5 353 30 45.4 5.4 

101 (+90) <10 <10 <10 80.2 80.5 81.8 <10 80.8 1.1 8.49 3.8 65.2 5.6

105 16.5 16.1 16.5 26.8 27.4 26.4 16.3 1.4 26.9 1.8 18.0 3.6 30.1 2.3 

118 60.1 59.6 60.2 80.3 81.7 80.0 60.0 0.6 80.7 1.1 58.4 7.5 74.6 5.1 128 9.44 9.19 9.25 20.0 21.1 20.1 9.29 1.4 20.4 3.0 12.0 1.2 23.7 1.7 132 w/153 w/153 w/153 w/153 w/153 w/153 w/153 w/153 <8 -- 22.5 4.6

138 (+163+164) 218 207 213 136 136 140 213 2.4 137 1.6 229 39.0 131.5 7.4 149 2.6 2.5 2.6 71.2 72.1 71.3 2.57 3.1 71.5 0.7 2.66 0.6 107 8.4 

151 <2 <2 <2 23.6 24.1 24.3 <2 24.0 1.5 <8 -- 28.7 5.2

153 2058 2038 2068 242 246 243 2055 0.7 244 1.1 1607 256 213.0 19 

156 58.0 58.6 59.6 10.0 10.5 9.9 58.7 1.4 10.1 3.0 59.9 7.9 10.3 1.1 

170 (+190) 435 439 444 46.9 47.7 47.1 440 1.0 47.2 0.8 309 53 40.6 2.6 

180 670 668 673 132.2 134.7 132.9 670 0.4 133 1.0 653 88 107 5.3 

183 20.9 20.3 21.1 36.4 36.8 36.6 20.8 2.0 36.6 0.6 24.3 4.8 36.6 4.1 

187 7.76 7.22 7.21 127 129 128 7.40 4.3 128 0.7 10.1 2.7 105 9.1 194 167 165 168 58.6 59.5 59.0 167 0.8 59.1 0.8 163 24 39.6 2.5 

195 3.46 2.99 3.37 10.2 10.8 10.9 3.27 7.6 10.6 3.7 4.18 1.1 17.7 4.3 

201 <2 <2 <2 11.7 11.8 11.9 <2 11.8 0.9 6.45 2.6 17.0 0.89

206 37.3 37.0 37.3 49.4 49.8 50.3 37.2 0.4 49.8 1.0 32.4 3.5 31.1 2.7 

209 12.5 12.3 12.2 17.7 18.4 17.2 12.3 1 17.8 3.5 12.3 1.1 10.6 1.1 66 3.44 2.78 3.13 22.6 23.5 21.5 3.12 11 22.5 4.6 <10 -- 23.6 1.695 <2 <2 <2 34.1 34.1 35.1 <2 34.4 1.6 <10 -- 33.8 1.7

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945 Homog. VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES La

SAMPLES: HOMOGENATE VI AND SRM 1945

data are reported as if three figures are significant

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

2,4’-DDT <5 <5 <5 95.1 92.6 96.7 <5 94.8 2.2 <5 -- 106 14

4,4’-DDT 5.44 5.36 5.41 222 230 227 5 0.7 226 1.8 8.83 2.2 245 15 

2,4’-DDE <5 <5 <5 12.7 12.3 12.4 <5 12.5 1.7 <5 -- 12.3 0.87

4,4’-DDE 58.2 61.1 60.4 475 457 463 59.9 2.5 465 2.0 72.3 8.0 445 37 

2,4’-DDD <5 <5 <5 18.6 18.3 18.7 <5 18.5 1.1 <5 -- 18.1 2.8

4,4’-DDD 4.26 4.55 4.32 136 142 133 4.38 3.5 137 3.3 3.71 1.6 133 10 

HCB 278 280 272 31.8 32.6 32.9 277 1.5 32.4 1.8 242 36 32.9 1.7 

α-HCH 68.3 64.6 68.8 16.9 16.1 16.7 67.2 3.4 16.6 2.5 65.4 11 16.2 3.4 

β-HCH 862 888 857 6.53 6.12 5.88 869 1.9 6.18 5.3 1102 184 8.00 1.4 

γ-HCH <15 <15 <15 <10 <10 <10 <15 <10 <5 -- 3.30 0.81

Heptachlor Epoxide 78.6 78.4 75.6 <10 <10 <10 77.5 2.2 <10 95.5 15 10.8 1.3 

Cis-Chlordane 24.8 26.7 25.2 49.6 43.7 46.8 25.6 3.9 46.7 6.3 22.1 29 46.9 2.8 Trans-Chlordane <5 <5 <5 11.0 11.1 10.9 <5 11.0 0.9 6.02 10 12.1 1.4

Oxychlordane 407 393 407 19.6 20.1 20.6 402 2.0 20.1 2.5 534 113 19.8 1.9 

Cis-Nonachlor 6.01 6.12 6.14 45.2 47.9 45.1 6.09 1.1 46.1 3.4 5.25 0.8 48.7 7.6 

Trans-Nonachlor 148 163 156 198 189 201 156 4.8 196 3.2 157 19 231 11 

Dieldrin 147 156 151 36.4 41.1 38.0 151 3.0 38.5 6.2 214 27 37.5 3.9 

Mirex 15.6 14.1 15.4 29.7 26.8 27.9 15.0 5.4 28.1 5.2 11.0 2.5 18.9 2.8 

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) 73.0 73.1 71.7 76.3 73.0 75.5 72.6 1.11 74.9 2.31 73.1 1.87 74.3 0.45 

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES LaSAMPLES: HOMOGENATE VI AND SRM 1945

data are reported as if three figures are significant

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 <5 <5 <5 <5 <5 <5 <5 <5 <5 -- 4.48 0.88

28 <6 <6 <6 14.9 14.8 13.9 <6 14.5 3.8 <5 -- 14.10 1.4

31 <5 <5 <5 3.50 3.64 3.65 <5 3.60 2.3 <5 -- 3.12 0.6944 <5 <5 <5 14.0 13.8 14.4 <5 14.1 2.2 <5 -- 12.2 1.449 21.8 21.7 22.7 18.7 18.7 19.3 22.1 2.5 18.9 1.8 <5 -- 20.8 2.8 

52 8.24 8.53 8.11 43.1 45.1 45.6 8.29 2.6 44.6 3.0 3.78 3.0 43.6 2.5 66/95* below below below below below below below below 3.44 2.7 57.4 2.5

87 <10 <10 <10 19.0 19.7 20.1 <10 19.6 2.8 6.02 1.2 16.7 1.4

99 321 309 307 51.9 51.5 52.4 312 2.4 51.9 0.9 353 30 45.4 5.4 

101 (+90) 4.97 5.01 4.89 72.1 70.7 71.4 4.96 1.2 71.4 1.0 8.49 3.8 65.2 5.6 

105 NA NA NA NA NA NA NA NA 18.0 3.6 30.1 2.3

118 73.7 71.3 75.3 74.0 75.7 79.8 73.4 2.7 76.5 3.9 58.4 7.5 74.6 5.1 

128 11.3 10.8 11.5 21.6 22.3 21.7 11.2 3.2 21.9 1.7 12.0 1.2 23.7 1.7 132 <8 <8 <8 19.1 20.3 20.7 <8 20.0 4.2 <8 -- 22.5 4.6

138 (+163+164) see notes see notes see notes see notes see notes see notes see notes see notes 229 39.0 131.5 7.4

149 <8 <8 <8 89.4 93.4 93.6 <8 92.1 2.6 2.66 0.6 107 8.4

151 <8 <8 <8 29.3 26.5 27.8 <8 27.9 5.0 <8 -- 28.7 5.2

153 2011 1955 2008 224 256 235 1991 1.6 238 6.8 1607 256 213.0 19 

156 66.3 63.8 66.5 9.92 10.4 10.2 65.5 2.3 10.2 2.4 59.9 7.9 10.3 1.1 

170 (+190) 354 344 338 40.5 40.9 40.3 345 2.3 40.6 0.8 309 53 40.6 2.6 

180 see notes see notes see notes see notes see notes see notes see notes see notes 653 88 107 5.3

183 21.6 22.0 22.3 35.5 36.2 36.3 22.0 1.6 36.0 1.2 24.3 4.8 36.6 4.1 

187 <10 <10 <10 112 115 109 <10 112 2.7 10.1 2.7 105 9.1

194 147 139 140 49.9 42.1 47.0 142 3.1 46.3 8.5 163 24 39.6 2.5 

195 <15 <15 <15 <15 <15 <15 <15 <15 4.18 1.1 17.7 4.3

201 NA NA NA NA NA NA NA NA 6.45 2.6 17.0 0.89

206 36.7 33.9 34.8 45.7 44.1 40.7 35.1 4.1 43.5 5.9 32.4 3.5 31.1 2.7 

209 12.5 11.9 12.6 14.4 14.4 13.8 12.3 3.1 14.2 2.4 12.3 1.1 10.6 1.1 66 <10 <10 <10 21.5 22.5 23.5 <10 22.5 4.4 <10 -- 23.6 1.6

95 <10 <10 <10 33.1 33.8 34.6 <10 33.8 2.2 <10 -- 33.8 1.7

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945 Homog. VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES La

SAMPLES: HOMOGENATE VI AND SRM 1945

data are reported as if three figures are significant

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

2,4’-DDT 43 36.8 38.8 70.4 70.3 66.6 40 8.0 69.1 3.1 <5 -- 106 14

4,4’-DDT <137 <137 <137 141 143 139 <137 141 1.4 8.83 2.2 245 15

2,4’-DDE <7.40 <7.40 <7.40 9.3 9.6 9.3 <7.40 9.40 1.8 <5 -- 12.3 0.87

4,4’-DDE 87.6 82.3 85.7 445 442 438 85.2 3.2 442 0.8 72 8.0 445 37 

2,4’-DDD <15.3 <15.3 <15.3 <15.3 <15.3 <15.3 <15.3 <15.3 <5 -- 18.1 2.8

4,4’-DDD <44.5 <44.5 <44.5 64.1 63.3 65.9 <44.5 64.4 2.1 3.71 1.6 133 10

HCB 207 200 184 17.6 18.4 17.6 197 6.0 17.9 2.6 242 36 32.9 1.7 

α-HCH 58.4 54.7 51.3 17.9 15.2 17.7 54.8 6.5 16.9 8.9 65.4 11 16.2 3.4 

β-HCH 1200 1190 1200 <59.4 <59.4 <59.4 1197 0.5 <59.4 1102 184 8.00 1.4 

γ-HCH <1 <1 <1 <1 <1 <1 <1 <1 <5 -- 3.30 0.81

Heptachlor Epoxide 102 102 98.7 10.4 9.1 9.6 101 1.9 9.70 6.8 95.5 15 10.8 1.3 

Cis-Chlordane <10.6 <10.6 <10.6 40.3 40.5 40.3 <10.6 40.4 0.3 22.1 29 46.9 2.8

Trans-Chlordane <41.4 <41.4 <41.4 <41.4 <41.4 <41.4 <41.4 <41.4 6.02 10 12.1 4.6

Oxychlordane NA NA NA NA NA NA NA NA 534 113 19.8 1.9

Cis-Nonachlor <7.20 <7.20 <7.20 31.4 32.9 33.1 <7.20 32.5 2.9 5.25 0.8 48.7 7.6

Trans-Nonachlor 182 175 180 170 170 169 179 2.0 170 0.3 157 19 231 11 

Dieldrin 217 222 220 <61.9 <61.9 <61.9 220 1.1 <61.9 214 27 37.5 3.9 

Mirex <10.4 <10.4 <10.4 21.4 21 21.5 <10.4 21.3 1.2 11.0 2.5 18.9 2.8

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) 71.0 71.0 71.0 71.0 71.0 71.0 71.0 0.00 71.0 0.00 73.1 1.87 74.3 0.45 

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES LaSAMPLES: HOMOGENATE VI AND SRM 1945

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 <8.20 <8.20 <8.20 <8.20 <8.20 <8.20 <8.20 <8.20 <5 -- 4.48 0.88

28 <11.3 <11.3 <11.3 11.8 12.6 12.9 <11.3 12.4 4.6 <5 -- 14.10 1.4

31 nd nd nd nd nd nd nd nd <5 -- 3.12 0.69

44 <10.5 <10.5 <10.5 <10.5 <10.5 <10.5 <10.5 <10.5 <5 -- 12.2 1.4

49 13.5 13.4 13.0 14.5 13.7 14.1 13.3 2.0 14.1 2.8 <5 -- 20.8 2.8 

52 <10.8 <10.8 <10.8 35.0 34.7 35.6 <10.8 35.1 1.3 3.78 3.0 43.6 2.5

66/95* below below below below below below below below 3.44 2.7 57.4 2.5

87 <9.4 <9.4 <9.4 19.9 19.3 19.5 <9.4 19.6 1.6 6.02 1.2 16.7 1.4

99 311 314 310 48.1 48.2 48.3 312 0.7 48.2 0.2 353 30 45.4 5.4 

101 (+90) 19.4 16.8 17.5 53.6 52.2 50.3 17.9 7.5 52.0 3.2 8.49 3.8 65.2 5.6 

105 <15.4 <15.4 <15.4 20.4 18.7 19.4 <15.4 19.5 4.4 18.0 3.6 30.1 2.3

118 49.6 48.4 45.0 69.7 66.4 66.3 47.7 5.0 67.5 2.9 58.4 7.5 74.6 5.1 

128 <55 <55 <55 <55 <55 <55 <55 <55 12.0 1.2 23.7 1.7

132 nd nd nd nd nd nd nd nd <8 -- 22.2 4.6

138 (+163+164) 89.2 84.8 83.2 61.7 59.3 57.2 85.7 3.6 59.4 3.8 229 39.0 131.5 7.4 

149 <11.3 <11.3 <11.3 73.9 67.8 71.2 <11.3 71.0 4.3 2.66 0.6 107 8.4

151 <8.90 <8.90 <8.90 32.0 29.5 29.1 <8.90 30.2 5.2 <8 -- 28.7 5.2153 1940 1920 1860 216 203 208 1907 2.2 209 3.1 1607 256 213.0 19 

156 51.5 50.2 45.3 <11.1 <11.1 <11.1 49.0 6.7 <11.1 59.9 7.9 10.3 1.1 

170 (+190) 174 171 171 31.9 31.5 29.5 172 1.0 31.0 4.2 309 53 40.6 2.6 

180 579 562 542 116 107 105 561 3.3 109 5.4 653 88 107 5.3 

183 <18.9 <18.9 <18.9 33.1 29.6 30.9 <18.9 31.2 5.7 24.3 4.8 36.6 4.1

187 <14.6 <14.6 <14.6 96.5 93.8 92.2 <14.6 94.2 2.3 10.1 2.7 105 9.1194 125 124 122 42.9 42.4 41.8 124 1.2 42.4 1.3 163 24 39.6 2.5 

195 <7.3 <7.3 <7.3 <7.3 <7.3 <7.3 <7.3 <7.3 4.18 1.1 17.7 4.3

201 <5.6 <5.6 <5.6 10.9 9.70 10.0 <5.6 10.2 6.1 6.45 2.6 17.0 0.89

206 27.4 26.1 25.1 35.3 34.1 33.4 26.2 4.4 34.3 2.8 32.4 3.5 31.1 2.7 

209 9.40 8.80 8.70 12.4 12.8 12.9 8.97 4.2 12.7 2.1 12.3 1.1 10.6 1.1 66 <11.7 <11.7 <11.7 18.4 17.2 17.3 <11.7 17.6 3.8 <10 -- 23.6 1.6

95 <9.70 <9.70 <9.70 30.7 28.9 30.9 <9.70 30.2 3.7 <10 -- 33.8 1.7

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945 Homog. VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES La

SAMPLES: HOMOGENATE VI AND SRM 1945

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

2,4’-DDT 1.74 1.85 1.81 89.1 88.4 88.6 1.80 3.1 89 0.4 <5 -- 106 14 

4,4’-DDT 6.87 7.29 6.91 259 257 256 7.02 3.3 257 0.6 8.83 2.2 245 15 2,4’-DDE <1.54 <1.58 <1.32 14.6 14.4 15.0 <1.54 14.7 2.1 <5 -- 12.3 0.87

4,4’-DDE 84.3 84.4 82.7 487 485 492 84 1.1 488 0.7 72 8.0 445 37 

2,4’-DDD <1.54 <1.58 <1.32 21.6 21.6 22.0 <1.54 21.7 1.1 <5 -- 18.1 2.8

4,4’-DDD 2.62 2.68 2.59 135 133 133 2.63 1.7 134 0.9 3.71 1.6 133 10 

HCB 273 271 265 27.2 26.9 27.5 270 1.5 27.2 1.1 242 36 32.9 1.7 

α-HCH 72.7 73.8 70.8 16.3 15.9 16.4 72.4 2.1 16.2 1.6 65.4 11 16.2 3.4 

β-HCH 1190 1230 1150 <1.81 <1.73 <1.66 1190 3.4 <1.81 1102 184 8.00 1.4 

γ-HCH 2.60 2.26 3.09 3.14 3.28 2.94 2.65 15.7 3.12 5.5 <5 -- 3.30 0.81 

Heptachlor Epoxide 97.4 103 99.2 12.4 12.3 12.4 100 2.9 12.4 0.5 95.5 15 10.8 1.3 

Cis-Chlordane <1.54 <1.58 <1.32 56.8 56.4 57.5 <1.54 56.9 1.0 22.1 29 46.9 2.8 

Trans-Chlordane 2.25 2.32 2.24 13.0 12.8 13.0 2.27 1.9 12.9 0.9 6.02 10 12.1 1.4 

Oxychlordane 483 476 467 24.0 22.2 22.9 475 1.7 23.0 3.9 534 113 19.8 1.9 

Cis-Nonachlor 6.43 6.35 6.16 58.7 58.1 58.4 6.31 2.2 58.4 0.5 5.25 0.8 48.7 7.6 

Trans-Nonachlor 184 184 179 182 181 183 182 1.6 182 0.5 157 19 231 11 

Dieldrin 228 233 221 56.6 55.0 54.7 227 2.7 55.4 1.8 214 27 37.5 3.9 

Mirex 11.8 12.2 11.5 38.4 38.1 38.5 11.8 3.0 38.3 0.5 11.0 2.5 18.9 2.8 

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) 72.8 68.6 72.0 73.6 73.2 73.8 71.1 3.14 73.5 0.42 73.1 1.87 74.3 0.45 

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES LaSAMPLES: HOMOGENATE VI AND SRM 1945

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 <1.55 <1.59 <1.33 3.14 3.08 3.04 <1.55 3.1 2 <5 -- 4.48 0.88

28 <1.56 <1.60 <1.34 9.84 9.74 9.96 <1.56 9.8 1 <5 -- 14.10 1.4

31 <1.17 <1.20 <1.00 4.38 4.26 4.25 <1.17 4.3 2 <5 -- 3.12 0.6944 <1.55 <1.59 <1.33 11.8 11.6 11.7 <1.55 11.7 1 <5 -- 12.2 1.4

49 <1.55 <1.59 <1.33 16.9 16.8 17.0 <1.55 16.9 1 <5 -- 20.8 2.852 1.78 1.80 1.84 38.2 38.0 38.2 1.8 1.7 38.1 0 3.78 3.0 43.6 2.5 

66/95* below below below below below below below below #DIV/0! 3.44 2.7 57.4 2.5

87 7.87 7.84 7.75 25.5 25.0 25.5 7.82 0.8 25.3 1 6.02 1.2 16.7 1.4 

99 320 319 317 54.9 54.5 55.1 319 0.5 54.8 1 353 30 45.4 5.4 

101 (+90) 4.81 4.86 4.92 77.5 76.9 78.1 4.86 1.1 77.5 1 8.49 3.8 65.2 5.6 

105 16.7 16.8 16.5 27.2 26.9 27.2 16.7 0.9 27.1 1 18.0 3.6 30.1 2.3 118 62.0 59.7 59.6 84.3 83.5 83.5 60.4 2.2 83.8 1 58.4 7.5 74.6 5.1 128 11.9 12.0 11.7 24.4 24.5 24.6 11.9 1.3 24.5 0 12.0 1.2 23.7 1.7 132 NA NA NA NA NA NA NA NA <8 -- 22.5 4.6

138 (+163+164) 288 288 282 183 182 185 286 1.2 183 1 229 39.0 131.5 7.4 

149 2.59 2.53 2.65 85.6 84.5 85.5 2.59 2.3 85.2 1 2.66 0.6 107 8.4 

151 <1.56 <1.60 <1.34 27.4 27.2 27.8 <1.56 27.5 1 <8 -- 28.7 5.2

153 1890 1890 1880 244 242 243 1887 0.3 243 0 1607 256 213.0 19 

156 64.1 64.7 63.0 12.6 12.5 12.7 63.9 1.3 12.6 1 59.9 7.9 10.3 1.1 

170 (+190) 411 414 404 50.1 50 50.7 409.7 1.3 50.3 1 309 53 40.6 2.6 180 733 739 731 144 143 144 734.3 0.6 144 0 653 88 107 5.3 183 23.4 23.7 23.1 39.8 39.3 39.8 23.4 1.3 39.6 1 24.3 4.8 36.6 4.1 187 8.72 8.87 8.65 115 113 115 8.75 1.3 114 1 10.1 2.7 105 9.1 194 155 158 155 60.7 60.6 61.1 156 1.1 60.8 0 163 24 39.6 2.5 

195 2.57 2.67 2.54 11.7 11.6 11.6 2.59 2.6 11.6 0 4.18 1.1 17.7 4.3 

201 NA NA NA NA NA NA NA NA 6.45 2.6 17.0 0.89

206 38.8 39.2 38.1 50.3 50.6 50.8 38.7 1.4 50.6 0 32.4 3.5 31.1 2.7 

209 12.9 12.9 12.8 18.4 18.4 18.6 12.9 0.4 18.5 1 12.3 1.1 10.6 1.1 66 <1.55 <1.59 <1.33 21.9 22.1 22.8 <1.55 22.3 2 <10 -- 23.6 1.6

95 0.960 0.984 1.01 40.0 39.7 40.1 0.98 2.5 39.9 1 <10 -- 33.8 1.7 

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945 Homog. VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES La

SAMPLES: HOMOGENATE VI AND SRM 1945

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

2,4’-DDT NA NA NA 39.7 36.7 39.5 NA 38.6 4.3 <5 -- 106 14

4,4’-DDT <2.2 <2.3 <2.0 209 183 180 <2.2 191 8.4 8.83 2.2 245 15

2,4’-DDE NA NA NA NA NA NA NA NA <5 -- 12.3 0.87

4,4’-DDE 88.2 89.6 85.9 574 548 507 87.9 2.1 543 6.2 72.3 8.0 445 37 

2,4’-DDD <1.7 <1.9 <1.6 24.9 27.1 23.8 <1.7 25.3 6.7 <5 -- 18.1 2.8

4,4’-DDD <3.5 <3.8 <3.3 119 106 112 <3.5 112 5.8 3.71 1.6 133 10

HCB 313 327 322 32.0 31.0 29.0 321 2.2 30.7 5.0 242 36 32.9 1.7 

α-HCH NA NA NA NA NA NA NA NA 65.4 11 16.2 3.4

β-HCH NA NA NA NA NA NA NA NA 1102 184 8.00 1.4

γ-HCH NA NA NA NA NA NA NA NA <5 -- 3.30 0.81

Heptachlor Epoxide NA NA NA NA NA NA NA NA 95.5 15 10.8 1.3

Cis-Chlordane NA NA NA NA NA NA NA NA 22.1 29 46.9 2.8Trans-Chlordane NA NA NA NA NA NA NA NA 6.02 10 12.2 1.4

Oxychlordane NA NA NA NA NA NA NA NA 534 113 19.8 1.9

Cis-Nonachlor NA NA NA NA NA NA NA NA 5.25 0.8 48.7 7.6Trans-Nonachlor NA NA NA NA NA NA NA NA 157 19 231 11

Dieldrin NA NA NA NA NA NA NA NA 214 27 37.5 3.9

Mirex NA NA NA NA NA NA NA NA 11.0 2.5 18.9 2.8

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) 54.3 57.2 62.2 67.4 56.3 55.8 57.9 6.90 59.8 10.96 73.1 1.87 74.3 0.45 

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES LaSAMPLES: HOMOGENATE VI AND SRM 1945

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 NA NA NA NA NA NA NA NA <5 -- 4.48 0.88

28 NA NA NA NA NA NA NA NA <5 -- 14.10 1.4

31 NA NA NA NA NA NA NA NA <5 -- 3.12 0.6944 NA NA NA NA NA NA NA NA <5 -- 12.2 1.4

49 NA NA NA NA NA NA NA NA <5 -- 20.8 2.852 NA NA NA NA NA NA NA NA 3.78 3.0 43.6 2.566/95* NA NA NA NA NA NA NA NA 3.44 2.7 57.4 2.587 NA NA NA NA NA NA NA NA 6.02 1.2 16.7 1.499 NA NA NA NA NA NA NA NA 353 30 45.4 5.4101 (+90) NA NA NA NA NA NA NA NA 8.49 3.8 65.2 5.6105 24.4 25.8 24.8 33.3 36.6 34.7 25.0 2.9 34.9 5 18.0 3.6 30.1 2.3 

118 71.0 69.2 66.6 125 121 115 68.9 3.2 120.3 4 58.4 7.5 74.6 5.1 128 NA NA NA NA NA NA NA NA 12.0 1.2 23.7 1.7

132 NA NA NA NA NA NA NA NA <8 -- 22.5 4.6

138 (+163+164) 211 217 215 130 135 138 214 1.4 134.3 3 229 39.0 131.5 7.4 

149 NA NA NA NA NA NA NA NA 2.66 0.6 107 8.4

151 NA NA NA NA NA NA NA NA <8 -- 28.7 5.2153 2270 2350 2330 298 300 292 2317 1.8 297 1 1607 256 213.0 19 156 74.2 79.1 77.1 6.90 7.40 9.32 76.8 3.2 7.9 16 59.9 7.9 10.3 1.1 

170 (+190) 513 536 529 101 103 94.1 526 2.2 99.4 5 309 53 40.6 2.6 

180 777 820 809 140 141 136 802 2.8 139 2 653 88 107 5.3 

183 NA NA NA NA NA NA NA NA 24.3 4.8 36.6 4.1

187 NA NA NA NA NA NA NA NA 10.1 2.7 105 9.1194 NA NA NA NA NA NA NA NA 163 24 39.6 2.5195 NA NA NA NA NA NA NA NA 4.18 1.1 17.7 4.3201 NA NA NA NA NA NA NA NA 6.45 2.6 17.0 0.89

206 NA NA NA NA NA NA NA NA 32.4 3.5 31.1 2.7

209 NA NA NA NA NA NA NA NA 12.3 1.1 10.6 1.166 NA NA NA NA NA NA NA NA <10 -- 23.6 1.6

95 NA NA NA NA NA NA NA NA <10 -- 33.8 1.7

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945 Homog. VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES La

SAMPLES: HOMOGENATE VI AND SRM 1945

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

Date(s) of measurements ng/g wet %RSD ng/g wet %RSD Value Value 

2,4’-DDT 14.7 24.9 16.9 150 96.6 102 18.9 29 116 25 <5 -- 106 14 

4,4’-DDT 14.7 26.8 6.53 353 434 418 16.0 64 402 11 8.83 2.2 245 15 2,4’-DDE 3.20 DL DL 22.1 28.3 30.4 3.20 26.9 16 <5 -- 12.3 0.87

4,4’-DDE 85.4 106 55.7 565 488 489 82.2 31 514 8.6 72 8.0 445 37 

2,4’-DDD 5.93 DL DL 35.5 28.6 28.8 5.93 31.0 13 <5 -- 18.1 2.8

4,4’-DDD see notes see notes see notes see notes see notes see notes see notes see notes 3.71 1.6 133 10

HCB 150 199 196 38.1 38.8 26.4 182 15 34.4 20 242 36 32.9 1.7 

α-HCH see notes see notes see notes see notes see notes see notes see notes see notes 65.4 11 16.2 3.4

β-HCH 1060 1450 765 25.6 24.7 4.35 1092 31 18.2 66 1102 184 8.00 1.4 

γ-HCH 4.11 6.85 3.06 24.4 21.7 6.31 4.67 42 17.5 56 <5 -- 3.30 0.81 

Heptachlor Epoxide 83.4 119 42.3 7.11 19.1 23.9 81.4 47 16.7 52 95.5 15 10.8 1.3 

Cis-Chlordane DL DL DL 62.5 69.0 50.2 60.5 16 22.1 29 46.9 2.8 

Trans-Chlordane 8.43 15.9 6.22 19.0 30.8 31.9 10.2 50 27.3 26 6.02 10 12.1 1.4 

Oxychlordane 438 618 272 23.7 25.7 19.5 443 39 22.9 14 534 113 19.8 1.9 

Cis-Nonachlor see notes see notes see notes see notes see notes see notes see notes see notes 5.25 0.8 48.7 7.6 

Trans-Nonachlor 158 219 72.4 197 201 160 150 49 186 12 157 19 231 11 

Dieldrin see notes see notes see notes see notes see notes see notes see notes see notes 214 27 37.5 3.9

Mirex 16.3 23.6 6.71 62.4 52.0 37.4 15.5 55 50.6 25 11.0 2.5 18.9 2.8 

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) 65.4 79.8 79.5 79.6 73.6 67.8 74.9 11.04 73.6 8.03 73.1 1.87 74.3 0.45 

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES LaSAMPLES: HOMOGENATE VI AND SRM 1945

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 6.76 9.81 3.35 DL DL 9.17 6.64 49 9.2 <5 -- 4.48 0.88 

28 2.74 5.27 2.47 24.1 16.3 28.6 3.49 44 23.0 27 <5 -- 14.10 1.4 

31 DL DL DL 13.8 14.6 16.9 DL 15.1 11 <5 -- 3.12 0.69 

44 8.51 9.33 2.89 20.6 22.2 18.9 6.91 51 20.5 8.0 <5 -- 12.2 1.4 

49 7.48 6.83 6.48 5.56 11.6 24.5 6.93 7 13.9 70 <5 -- 20.8 2.8 52 11.3 14.3 6.19 51.1 44.0 37.5 10.6 39 44.2 15 3.78 3.0 43.6 2.5 66/95* 7.81 9.93 6.67 67.8 74.0 69.7 8.14 20 70.5 4.5 3.44 2.7 57.4 2.5 87 see notes see notes see notes see notes see notes see notes see notes see notes 6.02 1.2 16.7 1.4

99 311 427 377 63.8 72.0 64.6 372 16 66.8 6.7 353 30 45.4 5.4 

101 (+90) 10.1 17.1 10.3 112 130 119 12.5 32 120 7.7 8.49 3.8 65.2 5.6 

105 16.5 22.1 17.3 46.0 66.3 65.6 18.6 16 59.3 19 18.0 3.6 30.1 2.3 118 62.8 87.4 59.3 99.8 106 98.0 69.8 22 101 4 58.4 7.5 74.6 5.1 128 11.9 15.0 11.5 37.7 52.1 35.6 12.8 15 41.8 22 12.0 1.2 23.7 1.7 132 see notes see notes see notes see notes see notes see notes see notes see notes <8 -- 22.5 4.6

138 (+163+164) 266 356 342 195 208 187 322 15 197 5.4 229 39.0 131.5 7.4 

149 3.03 5.03 5.60 91.3 83.1 76.9 4.6 30 83.8 8.7 2.66 0.6 107 8.4 

151 DL DL DL 33.4 29.8 25.3 DL 29.5 14 <8 -- 28.7 5.2

153 1380 1880 2130 246 255 232 1797 21 245 4.7 1607 256 213.0 19 

156 72.7 99.4 64.6 17.8 19.5 20.2 78.9 23 19.1 6.4 59.9 7.9 10.3 1.1 

170 (+190) 524 463 528 56.6 67.0 51.4 505 7 58.3 14 309 53 40.6 2.6 

180 664 894 972 192 222 186 843 19 200 10 653 88 107 5.3 

183 27.0 36.5 35.3 50.4 47.1 40.8 33.0 16 46.1 11 24.3 4.8 36.6 4.1 

187 12.0 16.2 15.6 309 155 132 14.6 16 199 48 10.1 2.7 105 9.1 194 190 259 232 81.2 86.8 84.9 227 15 84.3 3.4 163 24 39.6 2.5 

195 7.27 6.50 5.09 54.1 36.2 32.9 6.29 18 41.1 28 4.18 1.1 17.7 4.3 

201 10.1 14.5 15.3 101 83.8 79.2 13.3 21 87.9 13 6.45 2.6 17.0 0.89 

206 see notes see notes see notes see notes see notes see notes see notes see notes 32.4 3.5 31.1 2.7 

209 37.4 51.7 45.6 25.8 21.4 21.7 44.9 16 23.0 11 12.3 1.1 10.6 1.1 

66 NA NA NA NA NA NA NA NA <10 -- 23.6 1.6

95 NA NA NA NA NA NA NA NA <10 -- 33.8 1.7

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945 Homog. VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES La

SAMPLES: HOMOGENATE VI AND SRM 1945

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

2,4’-DDT 5.19 5.90 5.60 153 156 136 5.56 6.4 148 7.0 <5 -- 106 14 

4,4’-DDT 7.72 8.61 8.72 449 460 337 8.35 6.6 415 16.4 8.83 2.2 245 15 2,4’-DDE ND ND ND 14.7 13.0 12.3 ND 13.3 9.5 <5 -- 12.3 0.87

4,4’-DDE 152 167 159 829 792 712 159 4.8 778 7.7 72.3 8.0 445 37 

2,4’-DDD ND ND ND 33.7 30.3 28.0 ND 30.7 9.4 <5 -- 18.1 2.8

4,4’-DDD 3.52 3.60 3.75 177 226 190 3.62 3.2 198 13 3.71 1.6 133 10 

HCB 400 418 478 44.6 45.9 41.8 432 9.4 44.1 4.8 242 36 32.9 1.7 

a-HCH 105 97 124 21.8 20.4 18.1 109 13 20.1 9.3 65.4 11 16.2 3.4 b-HCH 1865 2019 2007 2.01 3.26 1.42 1964 4.4 2.23 42 1102 184 8.00 1.4 

g-HCH 2.72 2.83 3.01 3.655 3.639 3.149 2.86 5.2 3.48 8.3 <5 -- 3.30 0.81 

Heptachlor Epoxide 144 143 143 15.2 15.8 14.2 143 0.4 15.1 5.2 95.5 15 10.8 1.3 

Cis-Chlordane ND ND ND 81.4 79.8 69.0 ND 76.7 8.8 22.1 29 46.9 2.8

Trans-Chlordane 5.19 6.52 7.00 20.7 21.9 19.5 6.24 15.0 20.7 5.9 6.02 10 12.2 1.4 

Oxychlordane 618 675 676 25.4 24.8 21.6 656 5.1 24.0 8.6 534 113 19.8 1.9 

Cis-Nonachlor 7.40 7.14 8.40 57.6 57.8 48.7 7.65 8.7 54.7 9.5 5.25 0.8 48.7 7.6 

Trans-Nonachlor 298 309 346 307 316 268 318 8.0 297 8.7 157 19 231 11 

Dieldrin 271 295 307 52.9 55.7 49.3 291 6.3 52.6 6.0 214 27 37.5 3.9 

Mirex 152 146 168 34.7 34.1 24.0 155 7.5 30.9 19.5 11.0 2.5 18.9 2.8 

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) 71.7 69.3 73.9 74.0 66.9 69.3 71.6 3.21 70.1 5.15 73.1 1.87 74.3 0.45 

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES LaSAMPLES: HOMOGENATE VI AND SRM 1945

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 ND ND ND 4.30 3.25 1.71 ND 3.09 42 <5 -- 4.48 0.88 

28 7.15 10.6 7.71 15.9 16.3 13.2 8.50 22 15.1 11 <5 -- 14.10 1.4 

31 ND ND ND ND ND ND ND ND <5 -- 3.12 0.69

44 5.93 4.91 6.08 13.4 9.62 10.8 5.64 11 11.3 17 <5 -- 12.2 1.4 

49 11.6 10.3 5.41 35.1 41.0 31.3 9.13 36 35.8 14 <5 -- 20.8 2.8 52 9.18 11.9 9.39 55.8 58.9 52.6 10.2 15 55.8 5.6 3.78 3.0 43.6 2.5 66/95* 13.6 16.6 11.1 91.2 88.1 81.7 13.8 20 87.0 5.6 3.44 2.7 57.4 2.5 87 ND ND ND 34.9 35.8 30.7 ND 33.8 8.0 6.02 1.2 16.7 1.4 

99 434 489 475 70.2 69.7 64.0 466 6.1 68.0 5.1 353 30 45.4 5.4 

101 (+90) 26.0 29.3 28.5 98.3 97.5 89.6 27.9 6.1 95.2 5.1 8.49 3.8 65.2 5.6 

105 33.5 39.7 33.3 32.7 37.9 17.5 35.5 10 29.3 36 18.0 3.6 30.1 2.3 118 96.0 103 104 179 186 105 101 4.4 157 29 58.4 7.5 74.6 5.1 

128 16.0 18.6 15.7 28.7 32.1 10.7 16.8 10 23.8 48 12.0 1.2 23.7 1.7 

132 ND ND ND 31.5 34.2 16.5 ND 27.4 35 <8 -- 22.5 4.6 

138 (+163+164) 503 579 537 290 311 219 540 7.0 273 18 229 39.0 131.5 7.4 

149 245 273 264 156 155 101 261 5.6 137 23 2.66 0.6 107 8.4 

151 ND ND ND 59.0 80.8 47.3 ND 62.4 27 <8 -- 28.7 5.2 

153 3603 3897 4332 370 378 278 3944 9.3 342 16 1607 256 213.0 19 156 73.3 86.3 78.1 17.5 18.3 13.7 79.2 8.3 16.5 15 59.9 7.9 10.3 1.1 

170 (+190) 445 476 515 71.8 80.5 66.9 479 7.3 73.0 9.4 309 53 40.6 2.6 

180 1380 1476 1597 222 249 207 1484 7.3 226 9.4 653 88 107 5.3 

183 46.9 52.2 51.7 81.9 85.9 76.2 50.3 5.9 81.3 6.0 24.3 4.8 36.6 4.1 

187 8.50 9.51 8.84 118 127 108 8.95 5.7 117.7 7.8 10.1 2.7 105 9.1 194 279 323 301 87.1 101 68.6 301 7.2 85.5 19 163 24 39.6 2.5 

195 6.72 8.65 6.49 19.6 21.9 10.8 7.29 16 17.4 33 4.18 1.1 17.7 4.3 

201 81.4 94.6 84.7 24.1 26.8 23.5 86.9 7.9 24.8 7 6.45 2.6 17.0 0.89 

206 65.0 74.0 67.5 85.0 92.1 62.6 68.8 6.8 79.9 19 32.4 3.5 31.1 2.7 

209 25.8 28.9 28.5 32.4 35.1 26.9 27.8 6.2 31.5 13 12.3 1.1 10.6 1.1 66 see above see above see above see above see above see above see above see above <10 -- 23.6 1.695 see above see above see above see above see above see above see above see above <10 -- 33.8 1.7

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945 Homog. VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES La

SAMPLES: HOMOGENATE VI AND SRM 1945

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

Date(s) of measurements ng/g wet %RSD ng/g wet %RSD Value Value 

2,4’-DDT 2.44 4.17 2.15 91.9 92.6 93.5 2.92 37 92.6 0.9 <5 -- 106 14 

4,4’-DDT 5.80 8.82 5.73 219 216 213 6.79 26 216 1.4 8.83 2.2 245 15 2,4’-DDE 0.165 0.151 0.115 13.6 12.5 13.4 0.144 18 13.1 4.5 <5 -- 12.3 0.87 

4,4’-DDE 86.4 95.4 78.2 527 525 537 86.6 9.9 530 1.1 72.3 8.0 445 37 

2,4’-DDD 0.233 0.230 0.285 17.1 14.0 16.4 0.249 12 15.8 10 <5 -- 18.1 2.8 

4,4’-DDD 1.72 2.35 2.31 82.6 81.3 91.6 2.13 16 85.2 6.6 3.71 1.6 133 10 

HCB 297 316 273 26.2 26.7 25.9 295 7.3 26.3 1.5 242 36 32.9 1.7 

a-HCH 112 129 119 21.8 22.7 24.6 120 6.9 23.0 6.4 65.4 11 16.2 3.4 b-HCH 2900 3238 2615 4.36 2.81 2.51 2918 11 3.23 31 1102 184 8.00 1.4 

g-HCH 1.90 NA 1.72 3.11 3.79 4.15 1.81 7.1 3.68 14 <5 -- 3.30 0.81 

Heptachlor Epoxide 134 138 167 20.3 16.6 13.7 146 12 16.9 20 95.5 15 10.8 1.3 

Cis-Chlordane 92.0 101 95.4 125 106 87.2 96.3 4.9 106 18 22.1 29 46.9 2.8 

Trans-Chlordane 9.60 9.76 8.54 20.9 16.7 14.2 9.30 7.1 17.3 20 6.02 10 12.1 1.4 Oxychlordane 665 708 786 34.6 26.9 22.4 720 8.5 28.0 22 534 113 19.8 1.9 

Cis-Nonachlor 5.17 8.08 7.68 78.9 70.3 56.7 6.98 23 68.7 16 5.25 0.8 48.7 7.6 

Trans-Nonachlor 212 223 208 292 245 213 214 3.6 250 16 157 19 231 11 

Dieldrin 165 183 183 47.8 38.6 32.0 177 5.8 39.4 20 214 27 37.5 3.9 

Mirex 6.34 9.48 10.5 33.7 30.6 25.5 8.79 25 29.9 14 11.0 2.5 18.9 2.8 

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) 72.5 71.9 72.3 71.4 72.1 71.4 72.2 0.46 71.6 0.59 73.1 1.87 74.3 0.45 

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES LaSAMPLES: HOMOGENATE VI AND SRM 1945

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 DL DL 0.1 2.30 1.64 1.72 DL 1.89 19 <5 -- 4.48 0.88

28 0.336 0.248 0.257 9.33 8.78 9.24 0.280 17 9.1 3.3 <5 -- 14.10 1.4 

31 0.186 0.158 0.084 2.82 2.37 2.26 0.143 37 2.5 12 <5 -- 3.12 0.69 44 0.279 0.216 0.209 12.2 12.45 12.1 0.235 16 12.2 1.5 <5 -- 12.2 1.4 49 0.763 0.725 0.647 17.0 16.0 15.8 0.712 8.3 16.3 3.8 <5 -- 20.8 2.8 52 1.77 1.76 1.65 38.6 36.9 36.5 1.73 4.1 37.3 3.0 3.78 3.0 43.6 2.5 66/95* NA NA NA NA NA NA NA NA 3.44 2.7 57.4 2.5 

87 5.40 6.83 6.05 23.0 24.8 23.2 6.09 12 23.6 4.3 6.02 1.2 16.7 1.4 

99 386 410 395 60.8 63.6 59.4 397 3.0 61.3 3.5 353 30 45.4 5.4 

101 (+90) 4.33 4.23 4.32 88.4 91.1 85.9 4.29 1.3 88.5 2.9 8.49 3.8 65.2 5.6 

105 16.0 15.8 15.3 27.0 26.0 26.0 15.7 2.3 26.3 2.1 18.0 3.6 30.1 2.3 118 58.2 57.0 58.6 79.1 75.1 76.6 57.9 1.4 77 2.7 58.4 7.5 74.6 5.1 128 12.1 10.8 11.2 21.9 21.5 21.0 11.3 5.9 21.5 2.2 12.0 1.2 23.7 1.7 132 2951 2708 3071 305 286 297 2910 6.4 296.3 3.2 <8 -- 22.5 4.6 

138 (+163+164) 350 331 357 219 213 209 346 4.0 214 2.3 229 39.0 131.5 7.4 

149 2.51 2.44 2.46 107 95 101 2.47 1.3 101 6.1 2.66 0.6 107 8.4 

151 0.835 0.782 0.844 33.1 29.4 31.6 0.82 4.1 31.4 6.0 <8 -- 28.7 5.2 153 NA NA NA NA NA NA NA NA 1607 256 213.0 19

156 58.5 62.1 59.7 9.95 10.2 9.84 60.1 3.1 10.0 1.6 59.9 7.9 10.3 1.1 

170 (+190) 338 392 388 38.7 41.1 37.8 373 8.0 39.2 4.4 309 53 40.6 2.6 

180 882 862 862 150 162 147 869 1.4 153 5.0 653 88 107 5.3 

183 26.8 28.0 24.8 51.1 46.1 45.3 26.5 6.0 47.5 6.7 24.3 4.8 36.6 4.1 

187 9.63 10.00 8.84 159 154 156 9.49 6.3 156.5 1.5 10.1 2.7 105 9.1 194 180 165 165 61.2 63.1 56.1 170 5.1 60.1 6.0 163 24 39.6 2.5 

195 3.52 3.64 2.97 16.0 15.2 14.4 3.37 11 15.2 5.2 4.18 1.1 17.7 4.3 

201 5.01 4.94 4.82 91.6 89.7 85.3 4.92 2.0 88.9 3.6 6.45 2.6 17.0 0.89 

206 36.1 35.6 36.9 44.9 45.4 47.1 36.2 1.7 45.8 2.6 32.4 3.5 31.1 2.7 

209 14.0 13.1 12.8 17.8 17.7 17.7 13.3 4.8 17.8 0.3 12.3 1.1 10.6 1.1 66 1.07 1.06 1.06 24.2 22.8 23.2 1.06 0.5 23.4 3.1 <10 -- 23.6 1.6 95 0.878 0.946 0.836 39.7 38.4 38.1 0.887 6.3 38.7 2.2 <10 -- 33.8 1.7 

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945 Homog. VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES La

SAMPLES: HOMOGENATE VI AND SRM 1945

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

Date(s) of measurements ng/g wet %RSD ng/g wet %RSD Value Value 

2,4’-DDT NA NA NA NA NA NA NA NA <5 -- 106 14 

4,4’-DDT 6.78 6.53 5.07 183 241 305 6.13 15 243 25 8.83 2.2 245 15 

2,4’-DDE NA NA NA NA NA NA NA NA <5 -- 12.3 0.87 

4,4’-DDE 92.6 95.8 78.4 329 376 479 88.9 10 395 19 72.3 8.0 445 37 

2,4’-DDD NA NA NA NA NA NA NA NA <5 -- 18.1 2.8 

4,4’-DDD 4.37 3.91 3.39 69.8 113.0 130.0 3.89 13 104 30 3.71 1.6 133 10 

HCB 212 236 205 18.3 21.4 25.2 218 7.5 21.6 16 242 36 32.9 1.7 

a-HCH 73.3 76.6 60.7 11.1 13.4 16.3 70 12.0 13.6 19 65.4 11 16.2 3.4 b-HCH 1000 1000 892 1.45 1.73 2.02 964 6 1.73 16 1102 184 8.00 1.4 

g-HCH 2.43 2.51 2.08 2.12 2.49 2.90 2.34 9.8 2.50 16 <5 -- 3.30 0.81 

Heptachlor Epoxide 78.0 67.7 70.1 7.29 7.16 10.5 71.9 7.5 8.3 23 95.5 15 10.8 1.3 

Cis-Chlordane 1.98 1.50 1.20 34.0 43.7 54.1 1.56 25 43.9 23 22.1 29 46.9 2.8 Trans-Chlordane 45.4 47.0 38.8 10.8 14.0 18.6 43.7 9.9 14.5 27 6.02 10 12.2 1.4 Oxychlordane 517 491 342 14.2 12.3 23.3 450 21 16.6 35 534 113 19.8 1.9 

Cis-Nonachlor 7.26 7.10 6.17 28.4 51.0 55.1 6.84 9 44.8 32 5.25 0.8 48.7 7.6 

Trans-Nonachlor 181 181 141 114 130 182 168 14 142 25 157 19 231 11 

Dieldrin 240 233 195 36.0 46.4 57.6 223 11 46.7 23 214 27 37.5 3.9 

Mirex 11.1 12.3 10.3 23.2 27.9 34.2 11.2 9 28.4 19 11.0 2.5 18.9 2.8 

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) 65.2 69.4 67.0 59.2 68.6 70.5 67.2 3.14 66.1 9.10 73.1 1.87 74.3 0.45 

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES LaSAMPLES: HOMOGENATE VI AND SRM 1945

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 NA NA NA NA NA NA NA NA <5 -- 4.48 0.88

28 NA NA NA NA NA NA NA NA <5 -- 14.10 1.4

31 NA NA NA NA NA NA NA NA <5 -- 3.12 0.6944 0.750 1.40 1.13 8.17 9.10 11.0 1.09 30 9.4 15 <5 -- 12.2 1.4 

49 ND ND ND 12.4 13.8 17.2 ND 14.5 17 <5 -- 20.8 2.8 

52 4.09 4.62 3.86 25.8 29.4 36.5 4.19 9.3 30.6 18 3.78 3.0 43.6 2.5 

66/95* below below below below below below below below 3.44 2.7 57.4 2.5

87 NA NA NA NA NA NA NA NA 6.02 1.2 16.7 1.4

99 259 284 243 46.3 51.1 65.0 262 7.9 54.1 18 353 30 45.4 5.4 

101 (+90) 187 201 141 82.5 87.5 119 176 18 96.3 21 8.49 3.8 65.2 5.6 105 13.5 16.7 13.7 17.0 21.1 26.9 14.6 12 21.7 23 18.0 3.6 30.1 2.3 

118 60.4 67.9 57.3 58.3 71.3 84.1 61.9 8.8 71.2 18 58.4 7.5 74.6 5.1 128 10.4 12.4 10.3 14.6 18.4 21.8 11.0 10.7 18.3 20 12.0 1.2 23.7 1.7 132 NA NA NA 11.3 14.1 16.3 NA 13.9 18 <8 -- 22.5 4.6

138 (+163+164) 234 258 219 113 134 162 237 8.3 136 18 229 39.0 131.5 7.4 

149 NA NA NA 56.2 64.8 79.8 NA 66.9 18 2.66 0.6 107 8.4

151 0.870 1.110 0.930 18.3 20.6 25.2 0.970 12.9 21.4 16 <8 -- 28.7 5.2 

153 1301 1413 1195 143 167 204 1303 8.4 171.3 18 1607 256 213.0 19 

156 69.2 78.0 64.8 7.44 10.3 11.5 70.7 9.5 9.75 21 59.9 7.9 10.3 1.1 

170 (+190) 358 397 334 33.0 39.6 49.6 363 8.8 40.7 21 309 53 40.6 2.6 

180 592 649 548 95.9 115 142 596 8.5 118 20 653 88 107 5.3 

183 27.6 30.3 37.6 20.7 23.6 19.7 31.8 16 21.3 9.5 24.3 4.8 36.6 4.1 

187 NA NA NA NA NA NA NA NA 10.1 2.7 105 9.1

194 138 156 132 38.7 39 59.2 142 8.8 45.6 26 163 24 39.6 2.5 

195 3.34 3.75 3.15 21.1 21.9 31.6 3.41 9.0 24.9 24 4.18 1.1 17.7 4.3 

201 5.27 5.95 4.96 54.6 62.2 80.9 5.39 9.4 65.9 21 6.45 2.6 17.0 0.89 

206 33.5 37.5 31.8 32.6 33.2 49.8 34.3 8.5 38.5 25 32.4 3.5 31.1 2.7 

209 11.6 12.8 10.9 12.7 10.9 19.0 11.8 8.2 14.2 30 12.3 1.1 10.6 1.1 66 1.99 2.01 1.91 11.7 14.8 20.0 1.97 2.7 15.5 27 <10 -- 23.6 1.6 95 1.09 1.61 1.27 21.8 23.9 28.5 1.32 20 24.7 14 <10 -- 33.8 1.7 

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945 Homog. VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES La

SAMPLES: HOMOGENATE VI AND SRM 1945

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

Date(s) of measurements ng/g wet %RSD ng/g wet %RSD Value Value 

2,4’-DDT <2.60 <2.60 <2.60 120 140 153 <2.60 138 12.1 <5 -- 106 14

4,4’-DDT <=516 <=480 <=468 <=636 <=506 <=555 <=516 <=636 8.83 2.2 245 15

2,4’-DDE 24.4 22.5 22.4 14.5 13.8 15 23 4.9 14.4 4.2 <5 -- 12.3 0.87 

4,4’-DDE 7.85 7.47 7.47 455 444 478 7.6 2.9 459 3.8 72.3 8.0 445 37 

2,4’-DDD 10.7 10.4 11.5 21.1 25.2 24.8 10.9 5.2 23.7 9.5 <5 -- 18.1 2.8 

4,4’-DDD 3.33 4.4 <=2.60 89.8 150.1 163.8 3.87 20 135 29 3.71 1.6 133 10 

HCB 333 326 312 31.3 31.9 34 324 3.3 32.4 4.4 242 36 32.9 1.7 

α-HCH 9.10 12.2 6.22 17.0 16.8 16.8 9.2 33 16.9 0.7 65.4 11 16.2 3.4 

β-HCH 1240 1210 1000 <9.99 <9.99 <9.99 1150 11 <9.99 1102 184 8.00 1.4 

γ-HCH 2.46 2.42 1.63 2.97 2.41 2.37 2.17 22 2.58 13 <5 -- 3.30 0.81 

Heptachlor Epoxide 117 108 95.0 12.7 11.4 12.5 107 10 12.2 5.7 95.5 15 10.8 1.3 

Cis-Chlordane 23.2 23.6 21.8 64.2 68.6 66.6 22.9 4.1 66.5 3.3 22.1 29 46.9 2.8 

Trans-Chlordane 150 147 128 49.6 51.0 50.1 142 8.4 50.2 1.4 6.02 10 12.2 1.4 

Oxychlordane 788 725 606 24.3 20.4 24 706 13 22.9 9.5 534 113 19.8 1.9 

Cis-Nonachlor 2.96 3.65 3.68 30.2 46.3 50.4 3.43 12 42.3 25 5.25 0.8 48.7 7.6 

Trans-Nonachlor 200 186 189 205 213 227 192 3.8 215 5.2 157 19 231 11 

Dieldrin 287 316 221 51.5 51.4 50.0 275 18 51.0 1.6 214 27 37.5 3.9 

Mirex 69.9 63.2 65.2 37.9 39.7 32.5 66.1 5.2 36.7 10 11.0 2.5 18.9 2.8 

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) 76.8 73.8 71.1 81.2 75.3 86.4 73.9 3.88 81.0 6.85 73.1 1.87 74.3 0.45 

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES LaSAMPLES: HOMOGENATE VI AND SRM 1945

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 <9.69 <9.69 <9.69 <9.69 <9.69 <9.69 <9.69 <9.69 <5 -- 4.48 0.88

28 <9.69 <9.69 <9.69 8.36 8.99 9.51 <9.69 8.95 6.4 <5 -- 14.10 1.4

31 NA NA NA NA NA NA NA NA <5 -- 3.12 0.69

44 26.8 22.3 30.5 13.5 15.2 12.3 26.5 15 13.7 11 <5 -- 12.2 1.4 

49 NA NA NA NA NA NA NA NA <5 -- 20.8 2.8

52 19.1 22.7 25.0 49.6 45.7 45.3 22.3 13 46.9 5.1 3.78 3.0 43.6 2.5 

66/95* NA NA NA NA NA NA NA NA 3.44 2.7 57.4 2.5

87 <=105 <=101 <=105 <=455 <=443 <=478 <=105 <=455 6.02 1.2 16.7 1.4

99 NA NA NA NA NA NA NA NA 353 30 45.4 5.4

101 (+90) 12.4 16.0 6.01 60.7 57.6 60.4 11.5 44 59.6 2.9 8.49 3.8 65.2 5.6 

105 32.4 33.4 32.1 25.7 26.8 27.6 32.6 2.1 26.7 3.6 18.0 3.6 30.1 2.3 118 51.9 50.2 49.8 92.2 97.4 107 50.6 2.2 98.9 7.6 58.4 7.5 74.6 5.1 128 13.4 17.4 17.2 60.3 62.1 63.6 16.0 14 62.0 2.7 12.0 1.2 23.7 1.7 132 NA NA NA NA NA NA NA NA <8 -- 22.5 4.6

138 (+163+164) 307 278 243 209 207 211 276 12 209 1.0 229 39.0 131.5 7.4 

149 NA NA NA NA NA NA NA NA 2.66 0.6 107 8.4

151 NA NA NA NA NA NA NA NA <8 -- 28.7 5.2153 2100 2010 1880 258 267 301 1997 5.5 275 8.2 1607 256 213.0 19 156 NA NA NA NA NA NA NA NA 59.9 7.9 10.3 1.1 170 (+190) 296 270 268 32 32.3 33.5 278 5.6 32.6 2.4 309 53 40.6 2.6 

180 655 592 572 127 128 132 606 7.1 129 2.1 653 88 107 5.3 

183 NA NA NA NA NA NA NA NA 24.3 4.8 36.6 4.1

187 16.0 13.1 11.5 151 159 165 13.5 17 158 4.4 10.1 2.7 105 9.1 

194 237 220 226 70 81 77.1 228 3.8 76.0 7.3 163 24 39.6 2.5 

195 4.16 6.07 3.72 13.8 14 14.2 4.65 27 14.0 1.4 4.18 1.1 17.7 4.3 

201 <2.40 <2.40 <2.40 15.6 15.8 16.5 <2.40 16.0 3.0 6.45 2.6 17.0 0.89

206 46.1 45.4 44.6 63.7 71.1 71.2 45.4 1.7 68.7 6.3 32.4 3.5 31.1 2.7 

209 16.6 16.3 13.9 20.4 22.4 21.6 15.6 9.5 21.5 4.7 12.3 1.1 10.6 1.1 66 <9.69 <9.69 <9.69 41.7 39.5 44 <9.69 41.7 5.4 <10 -- 23.6 1.6

95 NA NA NA NA NA NA NA NA <10 -- 33.8 1.7

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945 Homog. VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES La

SAMPLES: HOMOGENATE VI AND SRM 1945

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

Date(s) of measurements ng/g wet %RSD ng/g wet %RSD Value Value 

2,4’-DDT 3.50 1.10 1.80 70.2 63.5 48.1 2.13 58 60.6 19 <5 -- 106 14 

4,4’-DDT 9.7 10.1 9.9 148 94 88 9.90 2.0 110 30 8.83 2.2 245 15 2,4’-DDE 5.30 5.80 6.00 10.4 7.4 9.1 5.70 6 9.0 17 <5 -- 12.3 0.87 

4,4’-DDE 43.4 42.8 41.5 396 351 297 42.6 2.3 348 14 72.3 8.0 445 37 

2,4’-DDD <1 <1 <1 45.5 43.2 33.8 <1 40.8 15 <5 -- 18.1 2.8

4,4’-DDD 1.20 1.10 <1 87.2 71.6 54.9 1.15 6.1 71.2 23 3.71 1.6 133 10 

HCB 179 173 189 23.6 20.0 19.8 180 4.5 21.1 10 242 36 32.9 1.7 

α-HCH 44.4 43.0 45.4 10.4 8.4 9.0 44.3 2.7 9.3 11 65.4 11 16.2 3.4 

β-HCH 672 671 720 2.30 2.00 1.70 688 4.1 2.00 15 1102 184 8.00 1.4 

γ-HCH 2.60 2.90 2.10 2.80 1.90 1.4 2.53 16 2.03 35 <5 -- 3.30 0.81 

Heptachlor Epoxide 49.2 48.0 51.0 8.80 8.4 7.4 49.4 3.1 8.20 8.8 95.5 15 10.8 1.3 

Cis-Chlordane 8.50 9.00 8.80 35.1 31.5 27.2 8.77 3 31.3 13 22.1 29 46.9 2.8 Trans-Chlordane 1.50 1.20 1.60 <1 <1 <1 1.43 15 <1 6.02 10 12.1 1.4 Oxychlordane 309 298 316 15.8 14.4 13.0 308 2.9 14.4 9.7 534 113 19.8 1.9 

Cis-Nonachlor 4.10 4.20 3.80 39.2 37.0 29.2 4.03 5.2 35.1 15 5.25 0.8 48.7 7.6 

Trans-Nonachlor 97.9 95.2 98.6 118 105 92 97.2 1.8 105 12 157 19 231 11 

Dieldrin 122 118 126 34.4 30.1 23.1 122 3.3 29.2 20 214 27 37.5 3.9 

Mirex 8.50 9.00 7.20 20.7 19.7 22.2 8.23 11 20.9 6.0 11.0 2.5 18.9 2.8 

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) 74.0 74.1 75.4 75.6 72.1 73.8 74.5 1.05 73.8 2.37 73.1 1.87 74.3 0.45 

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES LaSAMPLES: HOMOGENATE VI AND SRM 1945

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 5.10 8.50 6.90 2.30 2.60 1.80 6.83 25 2.23 18.1 <5 -- 4.48 0.88 

28 10.4 11.1 13.1 6.90 5.40 5.80 11.5 12 6.0 12.9 <5 -- 14.10 1.4 

31 NA NA NA NA NA NA NA NA <5 -- 3.12 0.69 

44 7.40 8.10 6.50 7.60 5.80 5.30 7.33 11 6.2 19.4 <5 -- 12.2 1.4 

49 NA NA NA NA NA NA NA NA <5 -- 20.8 2.8 

52 57.3 63.8 64.0 90.8 93.5 106.0 61.7 6 96.8 8.4 3.78 3.0 43.6 2.5 

66/95* 3.60 3.20 2.30 22.8 22.4 18.3 3.03 22 21.2 11.8 3.44 2.7 57.4 2.5 87 NA NA NA NA NA NA NA NA 6.02 1.2 16.7 1.4 

99 NA NA NA NA NA NA NA NA 353 30 45.4 5.4 101 (+90) 11.3 10.3 11.0 77.5 64.0 76.1 10.9 4.7 72.5 10 8.49 3.8 65.2 5.6 

105 10.5 12.3 14.7 25.7 29.2 26.4 12.5 17 27.1 6.8 18.0 3.6 30.1 2.3 118 42.1 38.0 43.0 53.7 53.9 42.5 41.0 6.5 50.0 13 58.4 7.5 74.6 5.1 128 8.80 8.60 9.00 19.2 19.1 15.6 8.80 2.3 18.0 11 12.0 1.2 23.7 1.7 132 NA NA NA NA NA NA NA NA <8 -- 22.5 4.6 

138 (+163+164) 110 107 116 124 86 75 111 4.1 94.9 27 229 39.0 131.5 7.4 

149 NA NA NA NA NA NA NA NA 2.66 0.6 107 8.4 

151 NA NA NA NA NA NA NA NA <8 -- 28.7 5.2 153 952 898 958 149 137 117 936 3.5 134 12 1607 256 213.0 19 156 NA NA NA NA NA NA NA NA 59.9 7.9 10.3 1.1 170 (+190) 233 232 229 27.8 23.7 18.2 231 0.9 23.2 21 309 53 40.6 2.6 

180 460 450 460 98.2 86.9 75.4 457 1.3 86.8 13 653 88 107 5.3 

183 NA NA NA NA NA NA NA NA 24.3 4.8 36.6 4.1 

187 5.40 5.60 6.10 92 90 66 5.70 6.3 83 17 10.1 2.7 105 9.1 

194 NA NA NA NA NA NA NA NA 163 24 39.6 2.5 

195 9.50 8.10 9.20 13.9 14.2 10.2 8.93 8.3 12.8 17 4.18 1.1 17.7 4.3 

201 NA NA NA NA NA NA NA NA 6.45 2.6 17.0 0.89 

206 25.9 26.3 25.3 31.9 32.1 30.3 25.8 1.9 31.4 3.1 32.4 3.5 31.1 2.7 

209 21.4 14.8 13.1 14.5 14.8 15.6 16.4 27 15.0 3.8 12.3 1.1 10.6 1.1 66 NA NA NA NA NA NA NA NA <10 -- 23.6 1.6 

95 NA NA NA NA NA NA NA NA <10 -- 33.8 1.7 

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945 Homog. VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES La

SAMPLES: HOMOGENATE VI AND SRM 1945

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

Date(s) of measurements ng/g wet %RSD ng/g wet %RSD Value Value 

2,4’-DDT <5 <5 <5 <5 <5 <5 <5 <5 <5 -- 106 14

4,4’-DDT <5 <5 <5 137 139 139 <5 138 0.83 8.83 2.2 245 152,4’-DDE <5 <5 <5 <5 <5 <5 <5 <5 <5 -- 12.3 0.87

4,4’-DDE 68.8 73.1 60.0 321 366 390 67.3 9.9 359 9.8 72.3 8.0 445 37 

2,4’-DDD <5 <5 <5 <5 <5 <5 <5 <5 <5 -- 18.1 2.8

4,4’-DDD <5 <5 <5 728 803 738 <5 756 5.4 3.71 1.6 133 10

HCB NA NA NA NA NA NA NA NA 242 36 32.9 1.7

α-HCH <5 <5 <5 <5 <5 <5 <5 <5 65.4 11 16.2 3.4

β-HCH 864 1100 1230 <10 <10 <10 1065 17 1230.0 1102 184 8.00 1.4 

γ-HCH <5 <5 <5 <5 <5 <5 <5 <5 <5 -- 3.30 0.81

Heptachlor Epoxide 68.9 98.8 110 <5 <5 <5 93 23 110.0 95.5 15 10.8 1.3 

Cis-Chlordane <5 <5 <5 37.3 35.0 41.4 <5 37.9 8.6 22.1 29 46.9 2.8

Trans-Chlordane NA NA NA NA NA NA NA NA 6.02 10 12.1 1.4

Oxychlordane 358 424 506 <10 18.7 29.8 429 17 24.3 32 534 113 19.8 1.9 

Cis-Nonachlor <5 <5 <5 37.2 35.4 36.4 <5 36.3 2.5 5.25 0.8 48.7 7.6

Trans-Nonachlor 172 185 130 169 191 154 162 18 171 11 157 19 231 11 

Dieldrin 197 199 232 569 434 555 209 9.4 519 14 214 27 37.5 3.9 

Mirex <5 <5 <5 <5 <5 <5 <5 <5 11.0 2.5 18.9 2.8

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) 69.5 73.4 73.6 71.2 73.4 75.6 72.2 3.2 73.4 3.0 73.1 1.87 74.3 0.45 

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES LaSAMPLES: HOMOGENATE VI AND SRM 1945

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 NA NA NA NA NA NA NA NA <5 -- 4.48 0.88

28 NA NA NA NA NA NA NA NA <5 -- 14.10 1.4

31 NA NA NA NA NA NA NA NA <5 -- 3.12 0.6944 NA NA NA NA NA NA NA NA <5 -- 12.2 1.4

49 NA NA NA NA NA NA NA NA <5 -- 20.8 2.852 NA NA NA NA NA NA NA NA 3.78 3.0 43.6 2.566/95* NA NA NA NA NA NA NA NA 3.44 2.7 57.4 2.587 NA NA NA NA NA NA NA NA 6.02 1.2 16.7 1.499 NA NA NA NA NA NA NA NA 353 30 45.4 5.4101 (+90) NA NA NA NA NA NA NA NA 8.49 3.8 65.2 5.6105 NA NA NA NA NA NA NA NA 18.0 3.6 30.1 2.3118 NA NA NA NA NA NA NA NA 58.4 7.5 74.6 5.1128 NA NA NA NA NA NA NA NA 12.0 1.2 23.7 1.7132 NA NA NA NA NA NA NA NA <8 -- 22.5 4.6

138 (+163+164) NA NA NA NA NA NA NA NA 229 39.0 131.5 7.4

149 NA NA NA NA NA NA NA NA 2.66 0.6 107 8.4151 NA NA NA NA NA NA NA NA <8 -- 28.7 5.2153 NA NA NA NA NA NA NA NA 1607 256 213.0 19

156 NA NA NA NA NA NA NA NA 59.9 7.9 10.3 1.1

170 (+190) NA NA NA NA NA NA NA NA 309 53 40.6 2.6180 NA NA NA NA NA NA NA NA 653 88 107 5.3183 NA NA NA NA NA NA NA NA 24.3 4.8 36.6 4.1187 NA NA NA NA NA NA NA NA 10.1 2.7 105 9.1194 NA NA NA NA NA NA NA NA 163 24 39.6 2.5195 NA NA NA NA NA NA NA NA 4.18 1.1 17.7 4.3201 NA NA NA NA NA NA NA NA 6.45 2.6 17.0 0.89

206 NA NA NA NA NA NA NA NA 32.4 3.5 31.1 2.7

209 NA NA NA NA NA NA NA NA 12.3 1.1 10.6 1.166 NA NA NA NA NA NA NA NA <10 -- 23.6 1.6

95 NA NA NA NA NA NA NA NA <10 -- 33.8 1.7

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945 Homog. VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES La

SAMPLES: HOMOGENATE VI AND SRM 1945

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

Date(s) of measurements ng/g wet %RSD ng/g wet %RSD Value Value 

2,4’-DDT <2.0 <2.0 <2.0 105 114 102 <2.0 107 5.8 <5 -- 106 14

4,4’-DDT 8.94 8.12 14.6 249 241 245 10.6 33.4 245 1.6 8.83 2.2 245 15 

2,4’-DDE <2.0 <2.0 <2.0 17.1 15.7 15.5 <2.0 16.1 5.4 <5 -- 12.3 0.87

4,4’-DDE 100 104 83.9 444 432 420 96.0 11 432 2.8 72.3 8.0 445 37 

2,4’-DDD <2.0 <2.0 <2.0 18.8 18.6 16.4 <2.0 17.9 7.4 <5 -- 18.1 2.8

4,4’-DDD 3.21 3.52 3.36 142 142 137 3.36 4.6 140 2.1 3.71 1.6 133 10 

HCB 167 193 161 23.5 24.6 22.5 174 9.8 23.5 4.5 242 36 32.9 1.7 

α-HCH 61.0 65.9 55.7 16.8 16.3 15.5 60.9 8.4 16.2 4.0 65.4 11 16.2 3.4 

β-HCH 1148 1129 966 9.34 8.32 7.22 1081 9.3 8.29 13 1102 184 8.00 1.4 

γ-HCH 2.60 3.15 2.78 3.49 3.32 2.98 2.84 10 3.26 8.0 <5 -- 3.30 0.81 

Heptachlor Epoxide 124 127 103 16.4 16.4 14.2 118 11 15.7 8.1 95.5 15 10.8 1.3 

Cis-Chlordane <2.0 <2.0 <2.0 59.3 54.8 51.8 <2.0 55.3 6.8 22.1 29 46.9 2.8

Trans-Chlordane <2.0 <2.0 <2.0 10.7 10.3 9.5 <2.0 10.2 6.0 6.02 10 12.1 1.4Oxychlordane 461 505 434 23.3 22.1 19.2 467 7.7 21.5 9.8 534 113 19.8 1.9 

Cis-Nonachlor 8.00 7.87 6.82 46.8 50.3 42.6 7.56 8.6 46.6 8 5.25 0.8 48.7 7.6 

Trans-Nonachlor 186 218 174 194 186 178 193 12 186 4.3 157 19 231 11 

Dieldrin Lost 200 195 39.4 44.1 47.4 198 1.8 43.6 9.2 214 27 37.5 3.9 

Mirex 12.0 14.0 17.1 27.4 29.7 28.4 14.37 18 28.5 4.0 11.0 2.5 18.9 2.8 

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) 68.4 68.2 63.9 70.3 70.1 70.4 66.8 3.80 70.3 0.22 73.1 1.87 74.3 0.45 

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES LaSAMPLES: HOMOGENATE VI AND SRM 1945

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 0.230 0.230 0.153 3.78 3.81 3.86 0.204 22 3.82 1.1 <5 -- 4.48 0.88 

28 0.934 1.19 0.920 10.2 10.4 10.3 1.01 15 10.3 1.0 <5 -- 14.10 1.4 

31 0.262 0.278 0.278 4.66 4.97 4.86 0.273 3.4 4.83 3.3 <5 -- 3.12 0.69 44 <0.100 <0.100 <0.100 11.4 10.9 11.7 <0.100 11.3 3.6 <5 -- 12.2 1.4

49 0.296 0.333 0.333 15.8 15.6 15.2 0.321 6.7 15.5 2.0 <5 -- 20.8 2.8 

52 0.324 0.278 0.278 41.7 42.3 41.1 0.293 9.1 41.7 1.4 3.78 3.0 43.6 2.5 66/95* below below below below below below below below 3.44 2.7 57.4 2.5

87 <0.1 <0.1 <0.1 24.0 26.1 23.0 <0.1 24.4 6.5 6.02 1.2 16.7 1.4

99 311 388 379 126 118 126 359 12 123 3.7 353 30 45.4 5.4 

101 (+90) 1.29 1.55 1.68 163 171 145 1.51 13 160 8.3 8.49 3.8 65.2 5.6 

105 20.4 19.9 18.2 49.7 49.5 48.0 19.5 5.9 49.1 1.9 18.0 3.6 30.1 2.3 118 68.4 84.0 80.6 103 101 105 77.7 11 103 1.9 58.4 7.5 74.6 5.1 128 12.4 13.8 12.6 33.1 30.4 27.9 12.9 5.9 30.5 8.5 12.0 1.2 23.7 1.7 132 <1.0 <1.0 <1.0 2.99 2.68 3.16 <1.0 2.9 8.3 <8 -- 22.5 4.6

138 (+163+164) 180 218 216 168 136 178 205 10 161 14 229 39.0 131.5 7.4 

149 3.63 4.44 3.67 94.6 93.1 93.2 3.91 12 93.6 0.9 2.66 0.6 107 8.4 

151 0.182 0.220 0.244 24.4 24.7 23.9 0.215 15 24.3 1.7 <8 -- 28.7 5.2 153 2078 1968 2042 245 234 240 2029 2.8 240 2.3 1607 256 213.0 19 

156 70.5 85.2 81.3 23.8 24.2 22.2 79.0 9.6 23.4 4.5 59.9 7.9 10.3 1.1 

170 (+190) 306 379 357 43.3 48.8 35.7 347 11 42.6 15 309 53 40.6 2.6 

180 964 918 947 163 164 159 943 2.5 162 1.6 653 88 107 5.3 

183 20.6 22.7 20.3 54.8 55.4 50.6 21.2 6.2 53.6 4.9 24.3 4.8 36.6 4.1 

187 9.13 10.30 9.37 134 131 132 9.60 6.4 132 1.2 10.1 2.7 105 9.1 194 154 178 169 54.9 70.8 38.1 167 7.3 54.6 30 163 24 39.6 2.5 

195 3.95 3.92 3.94 12.7 12.5 10.4 3.94 0.39 11.9 11 4.18 1.1 17.7 4.3 

201 6.17 5.86 5.25 125 124 129 5.76 8.1 126.0 2.1 6.45 2.6 17.0 0.89 

206 37.4 29.7 35.4 52.7 65.1 36.7 34.2 12 51.5 28 32.4 3.5 31.1 2.7 

209 14.0 11.3 13.7 14.8 18.2 10.8 13.0 11 14.6 25 12.3 1.1 10.6 1.1 66 0.306 0.306 0.278 29.1 31.1 27.9 0.297 5.4 29.4 5.5 <10 -- 23.6 1.6 95 <0.125 <0.125 <0.125 41.5 40.9 41.7 <0.125 41.4 1.0 <10 -- 33.8 1.7

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945 Homog. VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES La

SAMPLES: HOMOGENATE VI AND SRM 1945

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

Date(s) of measurements ng/g wet %RSD ng/g wet %RSD Value Value 

2,4’-DDT NA NA NA NA NA NA NA NA <5 -- 106 14

4,4’-DDT 76.1 69.2 81.0 178 176 234 75.4 7.9 196 17 8.83 2.2 245 15 

2,4’-DDE NA NA NA NA NA NA NA NA <5 -- 12.3 0.87

4,4’-DDE 58.9 58.1 52.9 342 320 257 56.6 5.8 306 14 72.3 8.0 445 37 

2,4’-DDD NA NA NA NA NA NA NA NA <5 -- 18.1 2.8

4,4’-DDD 13.1 13.1 14.5 138 122 143 13.6 6.0 134 8.2 3.71 1.6 133 10 

HCB NA NA NA NA NA NA NA NA 242 36 32.9 1.7

α-HCH 44.5 44.6 44.8 11.0 10.4 9.3 44.6 0.3 10.2 8.4 65.4 11 16.2 3.4 

β-HCH 606 650 634 10.8 8.90 7.30 630 3.5 9.00 19 1102 184 8.00 1.4 

γ-HCH NA NA NA NA NA NA NA NA <5 -- 3.30 0.81

Heptachlor Epoxide 62.1 57.7 80.9 12.1 10.4 9.4 66.9 18 10.6 13 95.5 15 10.8 1.3 

Cis-Chlordane 9.40 13.2 7.70 44.5 50.7 36.5 10.1 28 43.9 16 22.1 29 46.9 2.8 Trans-Chlordane NA NA NA NA NA NA NA NA 6.02 10 12.1 1.4

Oxychlordane 654 644 627 28.3 25.6 25.5 642 2.1 26.5 6.0 534 113 19.8 1.9 

Cis-Nonachlor NA NA NA NA NA NA NA NA 5.25 0.8 48.7 7.6

Trans-Nonachlor NA NA NA NA NA NA NA NA 157 19 231 11

Dieldrin 63.3 90.3 41.4 37.4 41.8 33.6 65.0 38 37.6 11 214 27 37.5 3.9 

Mirex NA NA NA NA NA NA NA NA 11.0 2.5 18.9 2.8

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) NA NA NA NA NA NA 73.1 1.87 74.3 0.45

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES LaSAMPLES: HOMOGENATE VI AND SRM 1945

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 NA NA NA NA NA NA NA NA <5 -- 4.48 0.88

28 NA NA NA NA NA NA NA NA <5 -- 14.10 1.4

31 NA NA NA NA NA NA NA NA <5 -- 3.12 0.6944 NA NA NA NA NA NA NA NA <5 -- 12.2 1.4

49 NA NA NA NA NA NA NA NA <5 -- 20.8 2.852 NA NA NA NA NA NA NA NA 3.78 3.0 43.6 2.566/95* NA NA NA NA NA NA NA NA 3.44 2.7 57.4 2.587 NA NA NA NA NA NA NA NA 6.02 1.2 16.7 1.499 NA NA NA NA NA NA NA NA 353 30 45.4 5.4101 (+90) NA NA NA NA NA NA NA NA 8.49 3.8 65.2 5.6105 NA NA NA NA NA NA NA NA 18.0 3.6 30.1 2.3118 NA NA NA NA NA NA NA NA 58.4 7.5 74.6 5.1128 NA NA NA NA NA NA NA NA 12.0 1.2 23.7 1.7132 NA NA NA NA NA NA NA NA <8 -- 22.5 4.6

138 (+163+164) NA NA NA NA NA NA NA NA 229 39.0 131.5 7.4

149 NA NA NA NA NA NA NA NA 2.66 0.6 107 8.4151 NA NA NA NA NA NA NA NA <8 -- 28.7 5.2153 NA NA NA NA NA NA NA NA 1607 256 213.0 19

156 NA NA NA NA NA NA NA NA 59.9 7.9 10.3 1.1

170 (+190) NA NA NA NA NA NA NA NA 309 53 40.6 2.6180 NA NA NA NA NA NA NA NA 653 88 107 5.3183 NA NA NA NA NA NA NA NA 24.3 4.8 36.6 4.1187 NA NA NA NA NA NA NA NA 10.1 2.7 105 9.1194 NA NA NA NA NA NA NA NA 163 24 39.6 2.5195 NA NA NA NA NA NA NA NA 4.18 1.1 17.7 4.3201 NA NA NA NA NA NA NA NA 6.45 2.6 17.0 0.89

206 NA NA NA NA NA NA NA NA 32.4 3.5 31.1 2.7

209 NA NA NA NA NA NA NA NA 12.3 1.1 10.6 1.166 NA NA NA NA NA NA NA NA <10 -- 23.6 1.6

95 NA NA NA NA NA NA NA NA <10 -- 33.8 1.7

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945 Homog. VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES La

SAMPLES: HOMOGENATE VI AND SRM 1945

data are reported as if three figures are significant

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

Date(s) of measurements ng/g wet %RSD ng/g wet %RSD Value Value 

2,4’-DDT NA NA NA 117 129 115 NA 120 6.1 <5 -- 106 14

4,4’-DDT 13.7 15.8 17.7 328 305 292 15.73 13 309 5.9 8.83 2.2 245 15 

2,4’-DDE NA NA NA NA NA NA NA NA <5 -- 12.3 0.87 

4,4’-DDE 72.1 81.8 79.1 538 524 513 77.7 6.5 525 2.3 72.3 8.0 445 37 

2,4’-DDD NA NA NA NA NA NA NA NA <5 -- 18.1 2.8

4,4’-DDD 14.0 4.31 4.60 126 130 102 7.65 72 119 13 3.71 1.6 133 10 

HCB 245 239 251 29.7 28.7 28.3 245 2.5 28.9 2.5 242 36 32.9 1.7 

a-HCH 82.1 77.9 77.1 12.0 12.4 9.42 79.0 3.4 11.3 14 65.4 11 16.2 3.4 

b-HCH NA NA NA NA NA NA NA NA 1102 184 8.00 1.4

g-HCH 3.15 3.29 2.98 2.33 2.41 1.83 3.1 5.0 2.19 14 <5 -- 3.30 0.81 

Heptachlor Epoxide NA NA NA 8.91 9.68 6.99 NA 8.5 16 95.5 15 10.8 1.3

Cis-Chlordane NA NA NA 33.5 30.5 25.4 NA 29.8 14 22.1 29 46.9 2.8

Trans-Chlordane NA NA NA 17.4 20.2 16.0 NA 17.8 12 6.02 10 12.1 4.6

Oxychlordane NA NA NA NA NA NA NA NA 534 113 19.8 1.9

Cis-Nonachlor NA NA NA NA NA NA NA NA 5.25 0.8 48.7 7.6Trans-Nonachlor NA NA NA NA NA NA NA NA 157 19 231 11

Dieldrin 233 209 214 32.4 39.1 33.6 219 5.7 35.0 10 214 27 37.5 3.9 

Mirex 15.4 22.5 21.4 52.3 44.5 43.3 19.77 19 46.7 10 11.0 2.5 18.9 2.8 

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) 72.1 71.6 73.7 71.7 71.9 69.9 72.5 1.46 71.1 1.54 73.1 1.87 74.3 0.45 

Data as Submitted by Laboratory (ng/g wet mass)

Homogenate VI SRM 1945

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Material Reference Values (ng/g wet mass)
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES LaSAMPLES: HOMOGENATE VI AND SRM 1945

data are reported as if three figures are significant

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 NA NA NA 2.68 2.73 2.65 NA 2.69 1.5 <5 -- 4.48 0.88

28 0.333 0.284 0.231 9.59 9.76 9.37 0.282 18 9.57 2.0 <5 -- 14.10 1.4 

31 2.11 1.94 1.74 3.83 4.05 3.86 1.93 10 3.92 3.1 <5 -- 3.12 0.69 44 2.16 2.19 1.94 10.3 10.4 10.0 2.10 6.6 10.2 1.7 <5 -- 12.2 1.4 49 2.44 2.22 2.22 14.1 14.1 13.6 2.29 5.6 13.9 2.2 <5 -- 20.8 2.8 52 3.08 2.90 2.70 26.4 26.4 25.4 2.89 6.5 26.1 2.3 3.78 3.0 43.6 2.5 66/95* 3.14 2.91 2.59 37.7 38.1 36.3 2.88 10 37.4 2.5 3.44 2.7 57.4 2.5 87 7.16 6.75 6.84 24.2 25.2 23.6 6.92 3.1 24.3 3.3 6.02 1.2 16.7 1.4 99 353 333 318 93.5 99.1 90.1 335 5.3 94.3 4.8 353 30 45.4 5.4 

101 (+90) 4.78 4.75 4.41 54.9 55.3 52.6 4.65 4.5 54.3 2.6 8.49 3.8 65.2 5.6 

105 12.6 11.8 12.0 18.0 19.2 18.4 12.1 3.3 18.5 3.3 18.0 3.6 30.1 2.3 118 47.5 44.4 44.2 60.3 59.8 57.8 45.3 4.1 59.3 2.2 58.4 7.5 74.6 5.1 128 18.0 16.9 NA 78.0 82.8 69.1 17.5 4.5 76.6 9.0 12.0 1.2 23.7 1.7 132 NA NA NA 15.0 14.9 13.9 NA 14.6 4.1 <8 -- 22.5 4.6

138 (+163+164) 183 169 167 113 113 109 173 5.0 112 1.9 229 39.0 131.5 7.4 

149 2.73 2.42 2.78 60.9 60.7 57.8 2.64 7.4 59.8 2.9 2.66 0.6 107 8.4 

151 NA NA NA NA NA NA NA NA <8 -- 28.7 5.2

153 1064 983 974 144 142 136 1007 4.9 141 2.9 1607 256 213.0 19 

156 NA NA NA NA NA NA NA NA 59.9 7.9 10.3 1.1

170 (+190) 280 256 257 34.5 34.7 34.3 264 5.1 34.5 0.7 309 53 40.6 2.6 

180 465 427 429 96.0 95.6 92.8 440 4.8 94.8 1.9 653 88 107 5.3 

183 18.8 17.3 16.9 29.1 27.9 27.0 17.7 5.5 28.0 3.7 24.3 4.8 36.6 4.1 

187 8.56 7.69 7.28 82.5 81.8 79.0 7.84 8.3 81.1 2.3 10.1 2.7 105 9.1 194 109 100 101 46.4 46.9 45.7 103 5.1 46.3 1.3 163 24 39.6 2.5 

195 2.47 2.36 2.44 6.05 5.43 5.86 2.42 2.3 5.78 5.5 4.18 1.1 17.7 4.3 

201 5.42 4.81 4.90 55.4 55.3 54.3 5.04 6.6 55.0 1.2 6.45 2.6 17.0 0.89 

206 27.1 25.4 25.5 33.8 35.1 34.4 26.0 3.8 34.4 1.9 32.4 3.5 31.1 2.7 

209 10.0 9.74 10.0 14.4 14.7 17.4 9.92 1.6 15.5 10 12.3 1.1 10.6 1.1 66 NA NA NA NA NA NA NA NA <10 -- 23.6 1.6

95 NA NA NA NA NA NA NA NA <10 -- 33.8 1.7

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945 Homog. VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES La

SAMPLES: HOMOGENATE VI AND SRM 1945

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

Date(s) of measurements ng/g wet %RSD ng/g wet %RSD Value Value 

2,4’-DDT <0.15 <0.15 <0.15 NA 79.2 75.8 <0.15 78 3.2 <5 -- 106 14

4,4’-DDT <0.83 <0.83 <0.83 262 253 247 <0.83 254 3.1 8.83 2.2 245 152,4’-DDE NA NA NA NA NA NA NA NA <5 -- 12.3 0.87

4,4’-DDE 63.6 62.2 73.6 469 469 500 66.5 9.4 479 3.8 72 8.0 445 37 

2,4’-DDD <0.14 <0.14 <0.14 NA 33.5 27.6 <0.14 30.6 14 <5 -- 18.1 2.8

4,4’-DDD 1.29 1.10 1.30 96.2 95.5 96.8 1.23 9.2 96.1 0.7 3.71 1.6 133 10 

HCB 241 231 260 23.5 24.1 24.2 244 6.0 23.9 1.6 242 36 32.9 1.7 

α-HCH 51.4 50.0 55.8 13.5 13.9 14.2 52.4 5.7 14 2.7 65.4 11 16.2 3.4 

β-HCH 959 934 1048 3.12 2.6 2.24 980 6.1 2.7 17 1102 184 8.00 1.4 

γ-HCH 2.07 1.92 1.91 2.23 2.29 2.19 1.97 4.7 2.2 2.2 <5 -- 3.30 0.81 

Heptachlor Epoxide <0.074 <0.074 <0.074 <0.074 <0.074 <0.074 <0.074 <0.074 95.5 15 10.8 1.3

Cis-Chlordane 73.9 80.4 89.6 60.5 62.2 61.4 81.3 10 61.4 1.5 22.1 29 46.9 2.8 

Trans-Chlordane 3.64 3.2 3.41 12.3 13.4 13.8 3.41 6.6 13.1 6.0 6.02 10 12.1 1.4 Oxychlordane 299 292 325 11.7 12.7 12.4 305 5.6 12.3 4.1 534 113 19.8 1.9 

Cis-Nonachlor 5.26 4.62 5.69 NA 45.2 46.3 5.19 10 46 1.7 5.25 0.8 48.7 7.6 

Trans-Nonachlor 146 154 181.1 162 167 168 160 11 166 2.0 157 19 231 11 

Dieldrin NA NA NA NA NA NA NA NA 214 27 37.5 3.9Mirex 10.2 10.0 10.53 35.8 35.4 33.8 10.3 2.4 35.0 3.2 11.0 2.5 18.9 2.8 

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) 75.2 73.4 78.9 73.5 71.2 78.0 75.8 3.72 74.2 4.68 73.1 1.87 74.3 0.45 

Data as Submitted by Laboratory (ng/g wet mass)

Homogenate VI SRM 1945

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Material Reference Values (ng/g wet mass)
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES LaSAMPLES: HOMOGENATE VI AND SRM 1945

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 NA NA NA NA NA NA NA NA NA <5 -- 4.48 0.88

28 <0.11 <0.11 <0.11 9.4 10.0 10.2 <0.11 <0.11 9.8 4.3 <5 -- 14.10 1.4

31 <0.16 <0.16 <0.16 <0.16 <0.16 <0.16 <0.16 <0.16 <0.16 <5 -- 3.12 0.6944 NA NA NA NA NA NA NA NA NA <5 -- 12.2 1.4

49 NA NA NA NA NA NA NA NA NA <5 -- 20.8 2.852 4.89 4.65 4.81 *** 36.8 36.4 4.8 3 36.6 0.9 3.78 3.0 43.6 2.5 

66/95* <0.13 <0.13 <0.13 20.4 21.7 21.3 <0.13 <0.13 21.1 3.0 3.44 2.7 57.4 2.5

87 <0.10 <0.10 <0.10 17.0 18.2 15.2 <0.10 <0.10 16.8 9.0 6.02 1.2 16.7 1.499 370 358 403 70.6 75.5 70.5 377 6.2 72.2 4.0 353 30 45.4 5.4 

101 (+90) 23.6 20.5 20.4 82 86 82 21.5 8.5 83.2 3.0 8.49 3.8 65.2 5.6 

105 21.2 20.3 22.9 34.0 34.8 34.1 21.5 6.3 34.3 1.3 18.0 3.6 30.1 2.3 118 57.5 55.3 62.6 71.4 74 73.7 58.5 6.4 72.9 1.8 58.4 7.5 74.6 5.1 128 13.6 13.5 14.4 *** 29.2 29.0 13.8 3.5 29.1 0.6 12.0 1.2 23.7 1.7 132 NA NA NA NA NA NA NA NA NA <8 -- 22.5 4.6

138 (+163+164) 273 267 335 174 176 175 292 13 175 0.5 229 39.0 131.5 7.4 

149 1.84 1.65 1.68 *** 55.7 54.3 1.72 5.7 55.0 1.8 2.66 0.6 107 8.4 

151 0.718 0.515 0.439 22.0 23.3 23.1 0.557 26 22.8 3.0 <8 -- 28.7 5.2 153 2165 2150 2434 232 236 230 2250 7.1 233 1.2 1607 256 213.0 19 

156 75.2 71.9 81.7 *** 12.8 12.7 76.3 6.6 12.7 0.2 59.9 7.9 10.3 1.1 

170 (+190) 410 406 466 42.3 43.7 42.7 427 7.8 42.9 1.7 309 53 40.6 2.6 

180 924 901 1049 169 164 158 958 8.3 164 3.2 653 88 107 5.3 

183 22.0 21.0 23.5 37.3 37.6 37.4 22.2 5.7 37.4 0.4 24.3 4.8 36.6 4.1 

187 5.72 5.38 6.16 80.2 83.7 81.9 5.75 6.8 81.9 2.1 10.1 2.7 105 9.1 194 205 207 245 83.2 88.1 90.7 219 10 87.3 4.3 163 24 39.6 2.5 

195 NA NA NA NA NA NA NA NA 4.18 1.1 17.7 4.3

201 NA NA NA NA NA NA NA NA 6.45 2.6 17.0 0.89

206 35.2 33.6 38.7 52.3 49.2 49.7 36 7.3 50.4 3.4 32.4 3.5 31.1 2.7 

209 11.6 11.4 13.1 19.0 18.4 18.6 12.1 7.8 18.6 1.5 12.3 1.1 10.6 1.1 

66 NA NA NA NA NA NA NA NA <10 -- 23.6 1.6

95 NA NA NA NA NA NA NA NA <10 -- 33.8 1.7

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945 Homog. VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES La

SAMPLES: HOMOGENATE VI AND SRM 1945

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

Date(s) of measurements ng/g wet %RSD ng/g wet %RSD Value Value 

2,4’-DDT 32.7 34.9 34.4 93.1 118.8 84.4 34.0 3.3 98.8 18 <5 -- 106 14 

4,4’-DDT 12.3 12.8 12.90 191 257 179 12.7 2.5 209 20 8.83 2.2 245 15 2,4’-DDE 5.30 6.4 5.2 7.44 9.60 8.56 5.64 12 8.53 13 <5 -- 12.3 0.87 

4,4’-DDE 110 112 98.9 418 529 440 107 6.6 462 13 72.3 8.0 445 37 

2,4’-DDD NA NA NA NA NA NA NA NA <5 -- 18.1 2.8

4,4’-DDD 4.75 4.02 4.27 114 158 117 4.34 8.5 129.6 19 3.71 1.6 133 10 

HCB 185 196 158 23.0 27.5 27.4 179 11 26.0 10 242 36 32.9 1.7 

α-HCH 63.8 67.3 56.5 14.2 17.7 15.9 62.5 8.8 15.9 11 65.4 11 16.2 3.4 

β-HCH 657 736 634 2.10 10.6 6.13 675 8.0 6.28 68 1102 184 8.00 1.4 

γ-HCH 5.10 6.50 5.21 3.20 4.02 3.60 5.60 14 3.61 11 <5 -- 3.30 0.81 

Heptachlor Epoxide NA NA NA NA NA NA NA NA 95.5 15 10.8 1.3

Cis-Chlordane NA NA NA NA NA NA NA NA 22.1 29 46.9 2.8Trans-Chlordane NA NA NA NA NA NA NA NA 6.02 10 12.1 1.4

Oxychlordane NA NA NA NA NA NA NA NA 534 113 19.8 1.9

Cis-Nonachlor NA NA NA NA NA NA NA NA 5.25 0.8 48.7 7.6Trans-Nonachlor NA NA NA NA NA NA NA NA 157 19 231 11

Dieldrin NA NA NA NA NA NA NA NA 214 27 37.5 3.9

Mirex NA NA NA NA NA NA NA NA 11.0 2.5 18.9 2.8

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) 70.2 74.7 72.8 75.0 75.6 74.5 72.6 3.08 75.0 0.69 73.1 1.87 74.3 0.45 

Data as Submitted by Laboratory (ng/g wet mass)

Homogenate VI SRM 1945

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Material Reference Values (ng/g wet mass)
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES LaSAMPLES: HOMOGENATE VI AND SRM 1945

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 NA NA NA NA NA NA NA NA <5 -- 4.48 0.88

28 13.5 12.8 12.9 9.27 11.00 8.70 13.1 2.9 9.66 12 <5 -- 14.10 1.4 

31 NA NA NA NA NA NA NA NA <5 -- 3.12 0.69

44 NA NA NA NA NA NA NA NA <5 -- 12.2 1.4

49 NA NA NA NA NA NA NA NA <5 -- 20.8 2.852 4.90 4.16 4.90 26.4 27.7 30.9 4.65 9.2 28.3 8.1 3.78 3.0 43.6 2.5 

66/95* NA NA NA NA NA NA NA NA 3.44 2.7 57.4 2.5

87 NA NA NA NA NA NA NA NA 6.02 1.2 16.7 1.499 NA NA NA NA NA NA NA NA 353 30 45.4 5.4101 (+90) 16.4 17.5 16.0 48.3 59.7 54.4 16.6 4.7 54 11 8.49 3.8 65.2 5.6 

105 19.0 18.8 18.1 29.8 37.4 25.2 18.6 2.5 30.8 20 18.0 3.6 30.1 2.3 118 137 148 134 82.2 102 82.0 139 5.5 88.7 13 58.4 7.5 74.6 5.1 

128 13.8 14.0 13.8 25.7 29.4 24.2 13.9 0.8 26.4 10 12.0 1.2 23.7 1.7 

132 NA NA NA NA NA NA NA NA <8 -- 22.5 4.6

138 (+163+164) 241 246 237 152 169 142 241 2 154 9.0 229 39.0 131.5 7.4 

149 0.600 3.20 2.00 66.9 80.2 69.8 1.93 67 72.3 9.7 2.66 0.6 107 8.4 

151 2.80 3.90 3.30 31.3 39.1 33.3 3.33 17 34.6 12 <8 -- 28.7 5.2 153 1386 1423 1225 206 245 197 1345 7.8 216 12 1607 256 213.0 19 

156 65.3 63.3 62.5 23.8 28.6 22.6 63.7 2.3 25.0 13 59.9 7.9 10.3 1.1 

170 (+190) 251 252 243 30.2 40.0 31.4 249 2.1 33.9 16 309 53 40.6 2.6 

180 511 493 471 124 144 113 492 4.1 127 13 653 88 107 5.3 

183 23.8 23.7 23.2 34.6 38.8 32.3 23.6 1.5 35.2 9 24.3 4.8 36.6 4.1 

187 20.2 19.3 19.4 102 118 98 19.6 2.5 106 10 10.1 2.7 105 9.1 194 123 120 117 50.3 78.8 60.6 120 2.4 63.2 23 163 24 39.6 2.5 

195 4.70 4.30 4.60 22.0 13.2 10.2 4.53 5 15.1 40 4.18 1.1 17.7 4.3 

201 6.30 6.20 8.60 65.9 78.9 63.4 7.03 19 69.4 12 6.45 2.6 17.0 0.89 

206 32.4 32.3 30.2 32.4 52.0 37.8 31.6 3.9 40.7 25 32.4 3.5 31.1 2.7 

209 12.2 11.7 11.7 15.7 26.6 18.0 11.9 2.6 20.1 29 12.3 1.1 10.6 1.1 66 NA NA NA NA NA NA NA NA <10 -- 23.6 1.6

95 94.0 85.2 83.6 37.8 39.8 41.0 87.6 6.4 39.5 4 <10 -- 33.8 1.7 

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945 Homog. VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES La

SAMPLES: HOMOGENATE VI AND SRM 1945

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

Date(s) of measurements ng/g wet %RSD ng/g wet %RSD Value Value 

2,4’-DDT <1.0 <1.0 <1.0 96.2 99.9 93.2 <1.0 96.4 3.5 <5 -- 106 14

4,4’-DDT 11.5 11.9 12.60 276 314 301 12.0 4.6 297 6.5 8.83 2.2 245 15 

2,4’-DDE NA NA NA NA NA NA NA NA <5 -- 12.3 0.87

4,4’-DDE 91 97 101.4 552 556 545 96.4 5.2 551 1.0 72.3 8.0 445 37 

2,4’-DDD <1.0 <1.0 <1.0 7.6 6.9 7.1 <1.0 7.20 5.0 <5 -- 18.1 2.8

4,4’-DDD 5.98 5.72 5.88 133 148 144 5.86 2.2 142 5.6 3.71 1.6 133 10 

HCB 244 243 259 28.3 28.3 29.2 248 4 28.6 1.8 242 36 32.9 1.7 

α-HCH 56.3 58.2 58.3 14.1 14.1 14.5 57.6 2.0 14.2 1.6 65.4 11 16.2 3.4 

β-HCH 1606 1636 1708 1.75 1.9 1.80 1650 3.2 1.81 3.3 1102 184 8.00 1.4 

γ-HCH 1.05 2.17 1.09 3.23 3.95 2.92 1.44 44 3.37 16 <5 -- 3.30 0.81 

Heptachlor Epoxide NA NA NA NA NA NA NA NA 95.5 15 10.8 1.3

Cis-Chlordane NA NA NA NA NA NA NA NA 22.1 29 46.9 2.8Trans-Chlordane NA NA NA NA NA NA NA NA 6.02 10 12.1 1.4

Oxychlordane 503 496 526 23.1 20.5 23.0 508 3.1 22.2 6.6 534 113 19.8 1.9 

Cis-Nonachlor NA NA NA NA NA NA NA NA 5.25 0.8 48.7 7.6

Trans-Nonachlor NA NA NA NA NA NA NA NA 157 19 231 11

Dieldrin NA NA NA NA NA NA NA NA 214 27 37.5 3.9

Mirex NA NA NA NA NA NA NA NA 11.0 2.5 18.9 2.8

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) 82.5 82.1 82.3 77.4 78.1 78.7 82.3 0.24 78.1 0.83 73.1 1.87 74.3 0.45 

Data as Submitted by Laboratory (ng/g wet mass)

Homogenate VI SRM 1945

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Material Reference Values (ng/g wet mass)
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES LaSAMPLES: HOMOGENATE VI AND SRM 1945

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 <0.5 <0.5 <0.5 2.30 2.24 2.33 <0.5 2.29 2.0 <5 -- 4.48 0.88

28 0.260 0.670 0.370 10.3 14.1 10.4 0.433 49 11.6 19 <5 -- 14.10 1.4 

31 <0.5 <0.5 <0.5 3.1 2.1 3.4 <0.5 2.85 23 <5 -- 3.12 0.69

44 <0.5 <0.5 <0.5 11.6 11.8 12.7 <0.5 12.0 4.9 <5 -- 12.2 1.4

49 0.710 0.940 0.440 17.30 16.2 16.7 0.697 36 16.7 3.3 <5 -- 20.8 2.8 

52 1.60 1.73 1.55 37.5 39.4 37.3 1.63 5.7 38.1 3.0 3.78 3.0 43.6 2.5 66/95* below below below below below below below below 3.44 2.7 57.4 2.5

87 6.16 5.32 5.52 24.9 23.0 23.6 5.67 7.7 23.8 4.1 6.02 1.2 16.7 1.4 

99 324 344 358 57.0 53.8 53.5 342 5.0 54.8 3.5 353 30 45.4 5.4 

101 (+90) 4.62 4.78 5.32 76.8 76.8 79.0 4.91 7.5 77.5 1.6 8.49 3.8 65.2 5.6 

105 15.0 16.1 16.6 26.7 25.4 26.1 15.9 5.1 26.1 2.5 18.0 3.6 30.1 2.3 118 55.0 58.1 60.6 76.0 72.2 74.1 57.9 4.8 74.1 2.6 58.4 7.5 74.6 5.1 128 10.7 11.3 11.8 20.7 20.4 20.5 11.3 4.9 20.5 0.7 12.0 1.2 23.7 1.7 132 0.520 0.430 0.420 18.2 17.7 17.5 0.457 12 17.8 2.0 <8 -- 22.5 4.6 138 (+163+164) 237 229 238 136 131 134 235 2.1 134 1.9 229 39.0 131.5 7.4 

149 2.49 2.58 2.66 78.8 75.8 77.1 2.58 3.3 77.2 1.9 2.66 0.6 107 8.4 

151 0.89 0.94 0.95 23.1 22.2 22.6 0.927 3.5 22.6 2.0 <8 -- 28.7 5.2 153 1725 1815 1885 191 190 195 1808 4.4 192 1.4 1607 256 213.0 19 

156 65.5 69.5 72.4 10.8 10.9 10.5 69.1 5.0 10.7 1.9 59.9 7.9 10.3 1.1 

170 (+190) 369 356 367 36.4 37.6 37.0 364 1.9 37.0 1.6 309 53 40.6 2.6 

180 759 737 759 131 131 127 752 1.7 130 1.8 653 88 107 5.3 

183 23.7 22.9 23.6 34.4 35.4 34.6 23.4 1.9 34.8 1.5 24.3 4.8 36.6 4.1 

187 9.00 8.70 8.90 117 118 115 8.87 1.7 117 1.3 10.1 2.7 105 9.1 194 164 159 165 53.1 53.6 54.2 163 2.0 53.6 1.0 163 24 39.6 2.5 

195 2.88 3.18 4.47 8.81 9.43 8.86 3.51 24 9.03 3.8 4.18 1.1 17.7 4.3 

201 NA NA NA NA NA NA NA NA 6.45 2.6 17.0 0.89

206 NA NA NA NA NA NA NA NA 32.4 3.5 31.1 2.7

209 NA NA NA NA NA NA NA NA 12.3 1.1 10.6 1.166 0.610 1.18 0.800 20.6 20.9 20.8 0.863 34 20.8 0.736 <10 -- 23.6 1.6 

95 1.49 1.54 1.39 36.5 36.4 36.6 1.5 5.2 36.5 0.274 <10 -- 33.8 1.7 

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945 Homog. VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES La

SAMPLES: HOMOGENATE VI AND SRM 1945

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

Date(s) of measurements ng/g wet %RSD ng/g wet %RSD Value Value 

2,4’-DDT NA NA NA NA NA NA NA NA <5 -- 106 14

4,4’-DDT 6.83 NA NA 150 NA NA 6.83 150 8.83 2.2 245 15 

2,4’-DDE NA NA NA NA NA NA NA NA <5 -- 12.3 0.87

4,4’-DDE 45.8 NA NA 340 NA NA 45.8 340 72.3 8.0 445 37 

2,4’-DDD NA NA NA NA NA NA NA NA <5 -- 18.1 2.8

4,4’-DDD 1.90 NA NA 126 NA NA 1.90 126 3.71 1.6 133 10 

HCB 136 NA NA 14.8 NA NA 136 14.8 242 36 32.9 1.7 

α-HCH 48.0 NA NA 7.39 NA NA 48.0 7.39 65.4 11 16.2 3.4 

β-HCH 1283 NA NA 4.79 NA NA 1283 4.79 1102 184 8.00 1.4 

γ-HCH NA NA NA NA NA NA NA NA <5 -- 3.30 0.81

Heptachlor Epoxide NA NA NA NA NA NA NA NA 95.5 15 10.8 1.3

Cis-Chlordane DL NA NA 25.6 NA NA DL 25.6 22.1 29 46.9 2.8

Trans-Chlordane DL NA NA 6.64 NA NA DL 6.64 6.02 10 12.1 1.4

Oxychlordane 1172 NA NA 44.2 NA NA 1172 44.2 534 113 19.8 1.9 

Cis-Nonachlor 3.93 NA NA 25.7 NA NA 3.93 25.7 5.25 0.8 48.7 7.6 

Trans-Nonachlor 88.9 NA NA 82.9 NA NA 88.9 82.9 157 19 231 11 

Dieldrin NA NA NA NA NA NA NA NA 214 27 37.5 3.9

Mirex NA NA NA NA NA NA NA NA 11.0 2.5 18.9 2.8

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) 78.7 NA NA 74.6 NA NA 78.7 74.6 73.1 1.87 74.3 0.45 

Data as Submitted by Laboratory (ng/g wet mass)

Homogenate VI SRM 1945

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Material Reference Values (ng/g wet mass)
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES LaSAMPLES: HOMOGENATE VI AND SRM 1945

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 NA NA NA NA NA NA NA NA <5 -- 4.48 0.88

28 NA NA NA NA NA NA NA NA <5 -- 14.10 1.4

31 NA NA NA NA NA NA NA NA <5 -- 3.12 0.6944 34.1 NA NA NA NA NA 34.1 NA <5 -- 12.2 1.4

49 45.9 NA NA 9.22 NA NA 45.9 9.2 <5 -- 20.8 2.852 105 NA NA 25.4 NA NA 105 25.4 3.78 3.0 43.6 2.5 

66/95* NA NA NA NA NA NA NA NA 3.44 2.7 57.4 2.5

87 30.7 NA NA 6.91 NA NA 31 6.9 6.02 1.2 16.7 1.4 

99 75 NA NA 204 NA NA 75 204 353 30 45.4 5.4 101 (+90) 113 NA NA 14.1 NA NA 113 14 8.49 3.8 65.2 5.6 105 42.3 NA NA 13.3 NA NA 42.3 13.3 18.0 3.6 30.1 2.3 

118 135 NA NA 52.6 NA NA 135 53 58.4 7.5 74.6 5.1 

128 NA NA NA NA NA NA NA NA 12.0 1.2 23.7 1.7

132 92.5 NA NA 30.6 NA NA 92.5 30.6 <8 -- 22.5 4.6

138 (+163+164) 412 NA NA 330 NA NA 412 330 229 39.0 131.5 7.4 

149 196 NA NA 15.1 NA NA 196 15.1 2.66 0.6 107 8.4 

151 58.4 NA NA DL NA NA 58.4 NA <8 -- 28.7 5.2

153 548 NA NA 2442 NA NA 548 2442 1607 256 213.0 19 

156 30.4 NA NA 85.4 NA NA 30.4 85.4 59.9 7.9 10.3 1.1 

170 (+190) 72.5 NA NA 345 NA NA 72.5 345 309 53 40.6 2.6 180 318 NA NA 854 NA NA 318 854 653 88 107 5.3 

183 87.0 NA NA 27.6 NA NA 87.0 27.6 24.3 4.8 36.6 4.1 

187 295 NA NA 13 NA NA 295 13 10.1 2.7 105 9.1 

194 198 NA NA 263 NA NA 198 263 163 24 39.6 2.5 

195 34.2 NA NA 5.98 NA NA 34.2 6.0 4.18 1.1 17.7 4.3 

201 81.0 NA NA NA NA NA 81.0 NA 6.45 2.6 17.0 0.89 

206 NA NA NA NA NA NA NA NA 32.4 3.5 31.1 2.7

209 NA NA NA NA NA NA NA NA 12.3 1.1 10.6 1.166 79.3 NA NA 10.7 NA NA 79.3 10.7 <10 -- 23.6 1.6

95 53.7 NA NA 10.8 NA NA 53.7 10.8 <10 -- 33.8 1.7

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945 Homog. VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES La

SAMPLES: HOMOGENATE VI AND SRM 1945

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

Date(s) of measurements ng/g wet %RSD ng/g wet %RSD Value Value 

2,4’-DDT NA NA NA NA NA NA NA NA <5 -- 106 14

4,4’-DDT 16.6 NA NA 40.4 NA NA 16.6 40.4 8.83 2.2 245 15 

2,4’-DDE NA NA NA NA NA NA NA NA <5 -- 12.3 0.87

4,4’-DDE 55.2 NA NA 247 NA NA 55.2 247 72.3 8.0 445 37 

2,4’-DDD NA NA NA NA NA NA NA NA <5 -- 18.1 2.8

4,4’-DDD 10.5 NA NA 6.17 NA NA 10.5 6.17 3.71 1.6 133 10 

HCB NA NA NA NA NA NA NA NA 242 36 32.9 1.7

α-HCH NA NA NA NA NA NA NA NA 65.4 11 16.2 3.4β-HCH NA NA NA NA NA NA NA NA 1102 184 8.00 1.4

γ-HCH NA NA NA NA NA NA NA NA <5 -- 3.30 0.81

Heptachlor Epoxide NA NA NA NA NA NA NA NA 95.5 15 10.8 1.3

Cis-Chlordane NA NA NA NA NA NA NA NA 22.1 29 46.9 2.8Trans-Chlordane NA NA NA NA NA NA NA NA 6.02 10 12.1 1.4

Oxychlordane NA NA NA NA NA NA NA NA 534 113 19.8 1.9

Cis-Nonachlor NA NA NA NA NA NA NA NA 5.25 0.8 48.7 7.6Trans-Nonachlor NA NA NA NA NA NA NA NA 157 19 231 11

Dieldrin NA NA NA NA NA NA NA NA 214 27 37.5 3.9

Mirex NA NA NA NA NA NA NA NA 11.0 2.5 18.9 2.8

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) 80.0 NA NA 91.0 NA NA 80.0 91.0 73.1 1.87 74.3 0.45 

Data as Submitted by Laboratory (ng/g wet mass)

Homogenate VI SRM 1945

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Material Reference Values (ng/g wet mass)
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES LaSAMPLES: HOMOGENATE VI AND SRM 1945

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 NA NA NA NA NA NA NA NA <5 -- 4.48 0.88

28 1.79 NA NA 24.1 NA NA 1.79 24.1 <5 -- 14.10 1.4

31 NA NA NA NA NA NA NA NA <5 -- 3.12 0.69

44 NA NA NA NA NA NA NA NA <5 -- 12.2 1.4

49 NA NA NA NA NA NA NA NA <5 -- 20.8 2.852 7.82 NA NA 72.2 NA NA 7.82 72.2 3.78 3.0 43.6 2.5 

66/95* 1.97 NA NA 46.5 NA NA 1.97 46.5 3.44 2.7 57.4 2.5 87 NA NA NA NA NA NA NA NA 6.02 1.2 16.7 1.4

99 NA NA NA NA NA NA NA NA 353 30 45.4 5.4101 (+90) 6.76 NA NA 80.9 NA NA 6.76 80.9 8.49 3.8 65.2 5.6 

105 8.05 NA NA 11.9 NA NA 8.05 11.9 18.0 3.6 30.1 2.3 118 38.9 NA NA 47.5 NA NA 38.9 47.5 58.4 7.5 74.6 5.1 128 NA NA NA NA NA NA NA NA 12.0 1.2 23.7 1.7

132 ND NA NA 7.47 NA NA ND 7.5 <8 -- 22.5 4.6

138 (+163+164) 250 NA NA 138 NA NA 250 138 229 39.0 131.5 7.4 

149 ND NA NA 52.1 NA NA ND 52.1 2.66 0.6 107 8.4

151 NA NA NA NA NA NA NA NA <8 -- 28.7 5.2153 2173 NA NA 179 NA NA 2173 179 1607 256 213.0 19 156 49.8 NA NA 3.85 NA NA 49.8 3.9 59.9 7.9 10.3 1.1 

170 (+190) 202 NA NA 20.2 NA NA 202 20.2 309 53 40.6 2.6 

180 791 NA NA 110 NA NA 791 110 653 88 107 5.3 

183 12.8 NA NA 20.8 NA NA 12.8 20.8 24.3 4.8 36.6 4.1 

187 NA NA NA NA NA NA NA NA 10.1 2.7 105 9.1

194 136 NA NA 42.2 NA NA 136 42.2 163 24 39.6 2.5 

195 NA NA NA NA NA NA NA NA 4.18 1.1 17.7 4.3

201 NA NA NA NA NA NA NA NA 6.45 2.6 17.0 0.89

206 NA NA NA NA NA NA NA NA 32.4 3.5 31.1 2.7

209 NA NA NA NA NA NA NA NA 12.3 1.1 10.6 1.166 NA NA NA NA NA NA NA NA <10 -- 23.6 1.6

95 NA NA NA NA NA NA NA NA <10 -- 33.8 1.7

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945 Homog. VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES La

SAMPLES: HOMOGENATE VI AND SRM 1945

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

Date(s) of measurements ng/g wet %RSD ng/g wet %RSD Value Value 

2,4’-DDT NA NA NA NA NA NA NA NA <5 -- 106 14

4,4’-DDT NA NA NA NA NA NA NA NA 8.83 2.2 245 15

2,4’-DDE NA NA NA NA NA NA NA NA <5 -- 12.3 0.87

4,4’-DDE NA NA NA NA NA NA NA NA 72.3 8.0 445 37

2,4’-DDD NA NA NA NA NA NA NA NA <5 -- 18.1 2.8

4,4’-DDD NA NA NA NA NA NA NA NA 3.71 1.6 133 10

HCB NA NA NA NA NA NA NA NA 242 36 32.9 1.7

α-HCH NA NA NA NA NA NA NA NA 65.4 11 16.2 3.4

β-HCH NA NA NA NA NA NA NA NA 1102 184 8.00 1.4

γ-HCH NA NA NA NA NA NA NA NA <5 -- 3.30 0.81

Heptachlor Epoxide NA NA NA NA NA NA NA NA 95.5 15 10.8 1.3

Cis-Chlordane NA NA NA NA NA NA NA NA 22.1 29 46.9 2.8Trans-Chlordane NA NA NA NA NA NA NA NA 6.02 10 12.1 1.4

Oxychlordane NA NA NA NA NA NA NA NA 534 113 19.8 1.9

Cis-Nonachlor NA NA NA NA NA NA NA NA 5.25 0.8 48.7 7.6

Trans-Nonachlor NA NA NA NA NA NA NA NA 157 19 231 11

Dieldrin NA NA NA NA NA NA NA NA 214 27 37.5 3.9

Mirex NA NA NA NA NA NA NA NA 11.0 2.5 18.9 2.8

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) ____________________________________________________________ #DIV/0! #DIV/0! #DIV/0! #DIV/0! 73.1 1.87 74.3 0.45 

Data as Submitted by Laboratory (ng/g wet mass)

Homogenate VI SRM 1945

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Material Reference Values (ng/g wet mass)
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES LaSAMPLES: HOMOGENATE VI AND SRM 1945

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 NA NA NA NA NA NA NA NA <5 -- 4.48 0.88

28 NA NA NA NA NA NA NA NA <5 -- 14.10 1.4

31 NA NA NA NA NA NA NA NA <5 -- 3.12 0.6944 NA NA NA NA NA NA NA NA <5 -- 12.2 1.449 NA NA NA NA NA NA NA NA <5 -- 20.8 2.852 NA NA NA NA NA NA NA NA 3.78 3.0 43.6 2.5

66/95* NA NA NA NA NA NA NA NA 3.44 2.7 57.4 2.587 NA NA NA NA NA NA NA NA 6.02 1.2 16.7 1.499 NA NA NA NA NA NA NA NA 353 30 45.4 5.4

101 (+90) NA NA NA NA NA NA NA NA 8.49 3.8 65.2 5.6

105 NA NA NA NA NA NA NA NA 18.0 3.6 30.1 2.3118 NA NA NA NA NA NA NA NA 58.4 7.5 74.6 5.1128 NA NA NA NA NA NA NA NA 12.0 1.2 23.7 1.7132 NA NA NA NA NA NA NA NA <8 -- 22.5 4.6

138 (+163+164) NA NA NA NA NA NA NA NA 229 39.0 131.5 7.4

149 NA NA NA NA NA NA NA NA 2.66 0.6 107 8.4

151 NA NA NA NA NA NA NA NA <8 -- 28.7 5.2

153 NA NA NA NA NA NA NA NA 1607 256 213.0 19

156 NA NA NA NA NA NA NA NA 59.9 7.9 10.3 1.1

170 (+190) NA NA NA NA NA NA NA NA 309 53 40.6 2.6

180 NA NA NA NA NA NA NA NA 653 88 107 5.3183 NA NA NA NA NA NA NA NA 24.3 4.8 36.6 4.1

187 NA NA NA NA NA NA NA NA 10.1 2.7 105 9.1

194 NA NA NA NA NA NA NA NA 163 24 39.6 2.5

195 NA NA NA NA NA NA NA NA 4.18 1.1 17.7 4.3

201 NA NA NA NA NA NA NA NA 6.45 2.6 17.0 0.89

206 NA NA NA NA NA NA NA NA 32.4 3.5 31.1 2.7

209 NA NA NA NA NA NA NA NA 12.3 1.1 10.6 1.166 NA NA NA NA NA NA NA NA <10 -- 23.6 1.6

95 NA NA NA NA NA NA NA NA <10 -- 33.8 1.7

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945 Homog. VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES L

SAMPLES: HOMOGENATE VI AND SRM 1945

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

Date(s) of measurements ng/g wet %RSD ng/g wet %RSD Value Value 2,4’-DDT NA NA NA NA NA NA NA NA <5 -- 106 14

4,4’-DDT NA NA NA NA NA NA NA NA 8.83 2.2 245 15

2,4’-DDE NA NA NA NA NA NA NA NA <5 -- 12.3 0.87

4,4’-DDE NA NA NA NA NA NA NA NA 72.3 8.0 445 37

2,4’-DDD NA NA NA NA NA NA NA NA <5 -- 18.1 2.84,4’-DDD NA NA NA NA NA NA NA NA 3.71 1.6 133 10

HCB NA NA NA NA NA NA NA NA 242 36 32.9 1.7

α-HCH NA NA NA NA NA NA NA NA 65.4 11 16.2 3.4

β-HCH NA NA NA NA NA NA NA NA 1102 184 8.00 1.4

γ-HCH NA NA NA NA NA NA NA NA <5 -- 3.30 0.81

Heptachlor Epoxide NA NA NA NA NA NA NA NA 95.5 15 10.8 1.3Cis-Chlordane NA NA NA NA NA NA NA NA 22.1 29 46.9 2.8Trans-Chlordane NA NA NA NA NA NA NA NA 6.02 10 12.1 1.4Oxychlordane NA NA NA NA NA NA NA NA 534 113 19.8 1.9Cis-Nonachlor NA NA NA NA NA NA NA NA 5.25 0.8 48.7 7.6Trans-Nonachlor NA NA NA NA NA NA NA NA 157 19 231 11

Dieldrin NA NA NA NA NA NA NA NA 214 27 37.5 3.9Mirex NA NA NA NA NA NA NA NA 11.0 2.5 18.9 2.8

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) NA 85.0 71.4 95.0 94.2 82.9 78.2 12.30 90.7 7.46 73.1 1.87 74.3 0.45 

Data as Submitted by Laboratory (ng/g wet mass)

Homogenate VI SRM 1945

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Material Reference Values (ng/g wet mass)
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES L

SAMPLES: HOMOGENATE VI AND SRM 1945

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 NA NA NA NA NA NA NA NA <5 -- 4.48 0.88

28 NA NA NA NA NA NA NA NA <5 -- 14.10 1.431 NA NA NA NA NA NA NA NA <5 -- 3.12 0.6944 NA NA NA NA NA NA NA NA <5 -- 12.2 1.449 NA NA NA NA NA NA NA NA <5 -- 20.8 2.852 NA NA NA NA NA NA NA NA 3.78 3.0 43.6 2.566/95* NA NA NA NA NA NA NA NA 3.44 2.7 57.4 2.587 NA NA NA NA NA NA NA NA 6.02 1.2 16.7 1.499 NA NA NA NA NA NA NA NA 353 30 45.4 5.4101 (+90) NA NA NA NA NA NA NA NA 8.49 3.8 65.2 5.6105 NA NA NA NA NA NA NA NA 18.0 3.6 30.1 2.3118 NA NA NA NA NA NA NA NA 58.4 7.5 74.6 5.1128 NA NA NA NA NA NA NA NA 12.0 1.2 23.7 1.7132 NA NA NA NA NA NA NA NA <8 -- 22.5 4.6138 (+163+164) NA NA NA NA NA NA NA NA 229 39.0 131.5 7.4149 NA NA NA NA NA NA NA NA 2.66 0.6 107 8.4151 NA NA NA NA NA NA NA NA <8 -- 28.7 5.2153 NA NA NA NA NA NA NA NA 1607 256 213.0 19

156 NA NA NA NA NA NA NA NA 59.9 7.9 10.3 1.1170 (+190) NA NA NA NA NA NA NA NA 309 53 40.6 2.6180 NA NA NA NA NA NA NA NA 653 88 107 5.3183 NA NA NA NA NA NA NA NA 24.3 4.8 36.6 4.1187 NA NA NA NA NA NA NA NA 10.1 2.7 105 9.1194 NA NA NA NA NA NA NA NA 163 24 39.6 2.5195 NA NA NA NA NA NA NA NA 4.18 1.1 17.7 4.3201 NA NA NA NA NA NA NA NA 6.45 2.6 17.0 0.89

206 NA NA NA NA NA NA NA NA 32.4 3.5 31.1 2.7

209 NA NA NA NA NA NA NA NA 12.3 1.1 10.6 1.166 NA NA NA NA NA NA NA NA <10 -- 23.6 1.695 NA NA NA NA NA NA NA NA <10 -- 33.8 1.7

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945 Homog. VI SRM 1945
2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES L

SAMPLES: HOMOGENATE VI AND SRM 1945

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

Date(s) of measurements ng/g wet %RSD ng/g wet %RSD Value Value 2,4’-DDT 2.17 2.72 3.14 54.3 55.3 55.8 2.68 18 55.1 1.4 <5 -- 106 14 

4,4’-DDT 7.27 5.93 5.69 142 121 133 6.29 14 132 7.9 8.83 2.2 245 15 

2,4’-DDE 6.45 6.70 8.20 8.04 7.77 8.09 7.12 13 7.97 2.2 <5 -- 12.3 0.87 

4,4’-DDE 84.7 77.7 73.7 481 400 511 78.7 7.1 464 12 72.3 8.0 445 37 

2,4’-DDD 2.03 2.15 2.38 13.3 10.4 12.3 2.19 8.2 12.0 12 <5 -- 18.1 2.8 4,4’-DDD 4.68 4.85 7.43 79.1 76.3 80.8 5.65 27 78.7 2.9 3.71 1.6 133 10 

HCB 255 405 414 33.1 34.3 37.8 358 25 35.1 7.0 242 36 32.9 1.7 a-HCH 71.4 88.0 93.3 8.96 9.22 9.83 84.2 14 9.34 4.8 65.4 11 16.2 3.4 b-HCH 1113 1648 1635 9.95 9.15 6.63 1465 21 8.58 20 1102 184 8.00 1.4 g-HCH 4.46 8.83 6.90 2.88 4.25 4.31 6.73 33 3.82 21 <5 -- 3.30 0.81 

Heptachlor Epoxide 101 106 101 8.73 8.86 9.64 103 2.9 9.08 5.4 95.5 15 10.8 1.3 Cis-Chlordane 89.1 87.4 83.7 31.4 40.7 34.7 86.7 3.2 35.6 13 22.1 29 46.9 2.8 Trans-Chlordane 10.5 9.64 8.70 3.07 1.38 2.28 9.61 9.3 2.24 38 6.02 10 12.1 1.4 Oxychlordane 564 854 833 23.0 23.0 23.6 750 22 23.2 1.4 534 113 19.8 1.9 Cis-Nonachlor 5.73 4.66 5.40 35.5 32.4 36.7 5.26 10 34.9 6.3 5.25 0.8 48.7 7.6 Trans-Nonachlor 179 162 157 133 117 139 166 7.0 129 8.9 157 19 231 11 

Dieldrin 216 312 311 43.0 37.3 42.6 280 20 41.0 7.8 214 27 37.5 3.9 Mirex 8.20 5.90 5.23 17.9 14.2 15.6 6.44 24 15.9 12 11.0 2.5 18.9 2.8 

Category 

≤ 2 

2 to 3 

≥ 3 

Lipid (mass fraction (%)) 64.8 67.7 68.2 60.6 64.8 65.6 66.9 2.74 63.7 4.22 73.1 1.87 74.3 0.45 

Data as Submitted by Laboratory (ng/g wet mass)

Homogenate VI SRM 1945

Homogenate VI SRM 1945 SRM 1945 Homog. VI

Material Reference Values (ng/g wet mass)
aCertified values are in bold bSee text for explaination

2003 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES L

SAMPLES: HOMOGENATE VI AND SRM 1945

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Targetb 95% CL 

ng/g wet %RSD ng/g wet %RSD Value Value 

18 NA NA NA NA NA NA NA NA <5 -- 4.48 0.88

28 3.33 6.41 5.90 12.4 16.7 16.4 5.21 32 15.2 16 <5 -- 14.10 1.4 31 NA NA NA 2.19 2.40 3.26 NA 2.6 22 <5 -- 3.12 0.6944 7.50 7.92 6.25 13.6 13.10 14.7 7.22 12 13.8 5.9 <5 -- 12.2 1.4 49 3.62 10.6 14.3 17.0 20.2 25.5 9.52 57.1 20.9 21 <5 -- 20.8 2.8 52 1.92 2.35 2.81 28.8 32.8 34.0 2.4 19 31.9 8.4 3.78 3.0 43.6 2.5 66/95* below below below below below below below below 3.44 2.7 57.4 2.587 4.24 3.96 3.67 17.8 15.1 19.5 4 7 17.5 13 6.02 1.2 16.7 1.4 99 338 471 496 52.5 41.5 50.1 435 20 48.0 12 353 30 45.4 5.4 101 (+90) 12.8 19.9 25.3 40.5 42.1 45.1 19.3 33 42.6 5.5 8.49 3.8 65.2 5.6 105 26.3 24.3 18.1 14.9 16.1 15.2 22.9 19 15.4 4.1 18.0 3.6 30.1 2.3 118 54.7 49.0 45.6 57.4 53.1 58.3 50 9.3 56 4.9 58.4 7.5 74.6 5.1 128 10.5 9.4 9.0 26.7 21.1 24.7 9.6 7.8 24.2 12 12.0 1.2 23.7 1.7 132 NA NA NA NA NA NA NA NA <8 -- 22.5 4.6138 (+163+164) NA NA NA NA NA NA NA NA 229 39.0 131.5 7.4149 NA NA NA NA NA NA NA NA 2.66 0.6 107 8.4151 7.65 6.94 6.12 20.0 19.6 23.2 7 11 20.9 9.4 <8 -- 28.7 5.2 153 NA NA NA NA NA NA NA NA 1607 256 213.0 19

156 33.6 26.9 23.1 6.1 6.6 5.6 27.9 19 6.1 8.5 59.9 7.9 10.3 1.1 170 (+190) 278 374 366 24.7 21.1 20.3 340 16 22.0 11 309 53 40.6 2.6 180 593 797 779 122 91 103 723 16 105 15 653 88 107 5.3 183 31.9 26.9 25.2 23.9 18.6 17.9 28.0 13 20.1 16 24.3 4.8 36.6 4.1 187 24.6 20.5 19.2 100 81.3 94 21.4 13 91.7 11 10.1 2.7 105 9.1 194 155 107 98 34.5 26 29.0 120 26 29.7 15 163 24 39.6 2.5 195 NA NA NA NA NA NA NA NA 4.18 1.1 17.7 4.3201 NA NA NA NA NA NA NA NA 6.45 2.6 17.0 0.89

206 28.8 20.2 18.5 22.4 17.4 19.1 22.5 24 19.7 13 32.4 3.5 31.1 2.7 

209 13.0 9.4 8.4 11.8 8.8 10.2 10.3 24 10.3 15 12.3 1.1 10.6 1.1 66 4.83 4.11 4.52 27.1 17.2 24.7 4.49 8.1 23.0 22 <10 -- 23.6 1.6 95 0.965 1.61 2.14 35.5 38.0 36.5 1.572 37 36.7 3.5 <10 -- 33.8 1.7 

aCertified values are in bold bSee text for explaination Category 

≤ 2 

2 to 3 

≥ 3 

Homogenate VI SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Material Reference Values (ng/g wet mass)

Homogenate VI SRM 1945 Homog. VI SRM 1945
Lab A

FATTY ACID 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab 

%RSD %RSD

C12:0 0.492 0.478 NA 1.83 1.84 1.83 0.485 2.0 1.83 0.3

C14:0 18.4 18.4 NA 34.8 35.0 35.5 18.4 0.0 35.1 1.0

C15:0 2.25 2.23 NA 3.37 3.40 3.49 2.24 0.6 3.42 1.8

C16:0 64.3 64.9 NA 77.9 78.3 80.9 64.6 0.7 79.0 2.1

C17:0 2.47 2.46 NA 2.45 2.45 2.53 2.47 0.3 2.48 1.9

C18:0 21.4 21.6 NA 13.8 13.90 14.50 21.5 0.7 14.1 2.7

C20:0 0.958 0.921 NA 1.29 1.27 1.32 0.940 2.8 1.29 1.9

C16:1(n-7) 93.0 93.5 NA 63.5 63.5 65.2 93.3 0.4 64.1 1.5

C18:1(n-7) 55.7 55.6 NA 19.4 19.7 20.3 55.65 0.1 19.8 2.3

C18:1(n-9) 165 167 NA 150 150 155 166 0.9 152 1.9

C18:1(n-9) 20.4 20.5 NA 2.56 2.54 2.70 20.5 0.3 2.60 3.4

C20:1(n-7) NA NA NA NA NA NA NA NA NA NA

C20:1(n-9) 31.9 32.4 NA 50.7 51.4 53.5 32.2 1.1 51.9 2.8

C:20:1(n-11) 0.696 0.680 NA 2.60 2.68 2.75 0.688 1.6 2.68 2.8

C22:1(n-9) 3.55 3.50 NA 7.37 7.52 7.81 3.53 1.0 7.57 3.0

C22:1(n-11) NA NA NA NA NA NA NA NA NA NA

C24:1(n-9) NA NA NA NA NA NA NA NA NA NA

C18:2(n-6) 7.59 7.60 NA 8.80 8.84 8.97 7.60 0.1 8.87 1.0

C18:3(n-3) 1.72 1.70 NA 3.88 3.93 3.95 1.71 0.8 3.92 0.9

C18:3(n-6) 0.651 0.629 NA 0.212 0.215 0.206 0.64 2.4 0.211 2.2

C18:4(n-3) 1.81 1.78 NA 1.87 1.87 1.79 1.80 1.2 1.84 2.5

C20:2(n-6) 3.10 3.09 NA 2.58 2.67 2.72 3.10 0.2 2.66 2.7

C20:3(n-3) 0.536 0.523 NA 1.84 1.75 1.84 0.530 1.7 1.81 2.9

C20:4(n-6) 3.90 3.84 NA 2.69 2.66 2.66 3.87 1.1 2.67 0.6

C20:5(n-3) 16.6 16.8 NA 12.4 12.6 12.6 16.70 0.8 12.5 0.9

C22:2(n-6) 0.122 0.138 NA 0.223 0.256 0.203 0.130 8.7 0.227 11.8

C22:5(n-3) 48.0 48.5 NA 8.64 10.2 8.97 48.3 0.7 9.27 8.9

C22:6(n-3) 45.8 46.5 NA 40.5 41.6 40.9 46.2 1.1 41.0 1.4

Lipid (mass fraction (%)) 72.3 72.3 NA 72.8 74.1 73.4 72.3 0.00 73.4 0.89

Data as Submitted by Laboratory (mg/g wet mass)

Homogenate VI SRM 1945 Homog. VI SRM 1945
Lab B

FATTY ACID 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab 

%RSD %RSD

C12:0 0.295 0.279 0.297 1.55 1.07 1.02 0.295 3.4 1.21 24

C14:0 13.6 12.4 11.9 24.8 21.4 18.8 13.6 6.6 21.7 14

C15:0 1.73 1.55 1.57 2.96 2.29 2.25 1.73 5.7 2.96 13

C16:0 37.5 33.6 32.6 35.9 36.8 27.8 37.5 6.9 33.5 15

C17:0 1.65 1.52 1.45 1.93 1.46 1.46 1.65 6.0 1.62 17

C18:0 15.2 14.2 14.1 11.4 8.67 8.67 15.2 3.8 9.57 16

C20:0 0.497 0.493 0.501 0.870 0.657 0.671 0.497 0.8 0.870 14

C16:1(n-7) 70.5 63.6 62.4 56.7 42.0 41.1 70.5 6.2 46.6 19

C18:1(n-7) 43.2 39.5 40.1 16.4 12.9 12.9 40.9 4.9 14.1 14

C18:1(n-9) 180 169 165 181 140 138 180 4.4 153 16

C18:1(n-9) 6.12 6.06 5.80 2.69 2.28 2.36 5.99 2.8 2.4 9

C20:1(n-7) NA NA NA NA NA NA NA NA NA NA

C20:1(n-9) 22.6 21.2 20.5 37.8 27.1 26.6 21.4 4.8 30.5 21

C:20:1(n-11) 8.94 8.46 9.01 25.6 22.1 21.7 8.94 3.3 25.6 8

C22:1(n-9) 2.39 2.24 2.09 5.19 4.56 4.63 2.24 6.6 4.79 7.2

C22:1(n-11) 5.14 5.26 4.89 48.0 38.4 37.0 5.10 3.7 41.2 15

C24:1(n-9) NA NA NA NA NA NA NA NA NA NA

C18:2(n-6) 5.13 4.83 4.91 7.38 5.59 5.64 4.96 3.0 6.21 16

C18:3(n-3) 1.98 1.92 1.91 7.86 5.92 5.85 1.94 2.0 6.54 17

C18:3(n-6) 0.371 0.338 0.349 0.111 0.090 0.090 0.353 4.8 0.097 12

C18:4(n-3) NA NA NA NA NA NA NA NA NA NA

C20:2(n-6) 1.77 1.69 1.63 1.84 1.25 1.41 1.69 4.1 1.50 20

C20:3(n-3) NA NA NA NA NA NA NA NA NA NA

C20:4(n-6) NA NA NA NA NA NA NA NA NA NA

C20:5(n-3) 15.6 16.7 15.6 15.3 10.7 11.5 16.0 4.2 12.5 20

C22:2(n-6) 0.082 0.065 0.091 0.139 0.135 0.140 0.082 16 0.14 1.9

C22:5(n-3) 34.3 34.0 34.0 9.75 7.30 7.26 34.1 0.5 8.10 18

C22:6(n-3) 34.8 33.5 33.9 35.4 30.1 27.2 34.1 2 30.9 13

Lipid (mass fraction (%)) 69.1 69.5 68.8 74.5 72.10 77.4 69.1 0.49 74.6 3.53

Data as Submitted by Laboratory (mg/g wet mass)

Homogenate VI SRM 1945 Homog. VI SRM 1945
Lab C

FATTY ACID 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab 

%RSD %RSD

C12:0 0.767 0.760 0.81 2.86 2.93 2.95 0.767 3.4 2.91 1.6

C14:0 24.5 24.5 25.0 45.4 46.7 46.9 24.5 1.0 46.3 1.8

C15:0 3.23 3.23 3.27 4.56 4.68 4.72 3.23 0.7 4.56 1.8

C16:0 85.4 85.3 86.7 99.1 102.2 102.5 85.4 1.0 101 1.9

C17:0 3.14 3.13 3.21 3.47 3.46 3.49 3.14 1.4 3.47 0.5

C18:0 28.5 28.5 29.1 17.0 17.12 17.29 28.5 1.2 17.1 1.0

C20:0 1.08 0.89 0.89 1.08 1.15 1.11 1.08 10 1.08 3.4

C16:1(n-7) 125 125 128 82.5 83.8 84.3 125 1.1 83.5 1.1

C18:1(n-7) 80.4 80.3 81.7 26.9 27.6 27.7 80.8 1.0 27.4 1.6

C18:1(n-9) 223 222 227 191 196 197 223 1.3 195 1.6

C18:1(n-9) 3.09 3.08 3.25 1.14 1.24 1.14 3.14 3.2 1.18 5.0

C20:1(n-7) NA NA NA NA NA NA NA NA NA NA

C20:1(n-9) 46.3 46.3 47.2 67.2 69.2 69.6 46.6 1.0 68.7 1.9

C:20:1(n-11) NA NA NA NA NA NA NA NA NA NA

C22:1(n-9) 4.54 4.71 4.81 8.49 8.78 8.84 4.69 2.9 8.70 2.2

C22:1(n-11) NA NA NA NA NA NA NA NA NA NA

C24:1(n-9) 0.800 0.748 0.742 3.02 3.08 3.05 0.80 4.0 3.05 1.0

C18:2(n-6) 11.0 11.0 11.3 12.0 12.4 12.5 11.1 1.1 12.3 2.2

C18:3(n-3) 30.6 30.7 31.1 7.91 8.16 8.17 30.8 0.9 8.08 1.8

C18:3(n-6) 0.692 0.713 0.722 0.223 0.231 0.205 0.71 2.2 0.220 6.0

C18:4(n-3) NA NA NA NA NA NA NA NA NA NA

C20:2(n-6) 2.78 2.78 2.76 3.21 3.15 3.14 2.77 0.4 3.16 1.2

C20:3(n-3) NA NA NA NA NA NA NA NA NA NA

C20:4(n-6) 4.87 4.91 4.97 3.32 3.42 3.44 4.91 1.0 3.39 1.8

C20:5(n-3) 22.7 25.3 25.9 17.8 18.2 18.2 24.7 6.9 18.1 1.5

C22:2(n-6) NA NA NA NA NA NA NA NA NA NA

C22:5(n-3) 78.0 78.0 79.3 14.4 14.1 14.3 78.4 0.9 14.2 1.1

C22:6(n-3) 68.4 68.1 69.9 59.9 60.8 61.2 68.8 1.4 60.6 1.1

Data as Submitted by Laboratory (mg/g wet mass)

Homogenate VI SRM 1945 Homog. VI SRM 1945
Lab D

FATTY ACID 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab 

%RSD %RSD

C12:0 0.520 NA NA 0.464 NA NA 0.520 NA 0.464 NA

C14:0 3.45 NA NA 4.91 NA NA 3.45 NA 4.91 NA

C15:0 0.482 NA NA 0.511 NA NA 0.482 NA 0.511 NA

C16:0 10.2 NA NA 10.5 NA NA 10.2 NA 10.5 NA

C17:0 0.27 NA NA 0.37 NA NA 0.275 NA 0.368 NA

C18:0 2.39 NA NA 1.98 NA NA 2.39 NA 1.98 NA

C20:0 0.235 NA NA 0.257 NA NA 0.23 NA 0.257 NA

C16:1(n-7) 9.68 NA NA 8.30 NA NA 9.68 NA 8.30 NA

C18:1(n-7) ND NA NA ND NA NA NA NA NA

C18:1(n-9) 21.8 NA NA 17.1 NA NA 21.8 NA 17.1 NA

C18:1(n-9) 0.121 NA NA 0.15 NA NA 0.12 NA 0.154 NA

C20:1(n-7) NA NA NA NA NA NA NA NA NA

C20:1(n-9) 3.47 NA NA 5.47 NA NA 3.47 NA 5.47 NA

C:20:1(n-11) NA NA NA NA NA NA NA NA NA NA

C22:1(n-9) 0.586 NA NA 0.544 NA NA 0.586 NA 0.544 NA

C22:1(n-11) NA NA NA NA NA NA NA NA NA

C24:1(n-9) 0.370 NA NA 0.27 NA NA 0.370 NA 0.270 NA

C18:2(n-6) 0.904 NA NA 1.04 NA NA 0.904 NA 1.04 NA

C18:3(n-3) 0.445 NA NA 0.503 NA NA 0.445 NA 0.503 NA

C18:3(n-6) 0.091 NA NA 0.044 NA NA 0.091 NA 0.044 NA

C18:4(n-3) NA NA NA NA NA NA NA NA NA

C20:2(n-6) NA NA NA NA NA NA NA NA NA

C20:3(n-3) 0.203 NA NA 0.228 NA NA 0.203 NA 0.228 NA

C20:4(n-6) 0.760 NA NA 0.607 NA NA 0.760 NA 0.607 NA

C20:5(n-3) 2.23 NA NA 2.04 NA NA 2.23 NA 2.04 NA

C22:2(n-6) 0.010 NA NA 0.072 NA NA 0.010 NA 0.072 NA

C22:5(n-3) 0.799 NA NA 1.33 NA NA 0.799 NA 1.33 NA

C22:6(n-3) 5.53 NA NA 5.51 NA NA 5.53 NA 5.51 NA

Lipid (mass fraction (%)) 76.0 NA NA 73.0 NA NA 76.0 73.0

Data as Submitted by Laboratory (ng/g wet mass)

Homogenate VI SRM 1945 Homog. VI SRM 1945
Lab E

FATTY ACID 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab 

%RSD %RSD

C12:0 0.066 0.066 0.066 0.271 0.262 0.238 0.07 0.0 0.26 6.6

C14:0 2.63 2.62 2.62 5.23 5.13 4.97 2.63 0.2 5.11 2.6

C15:0 0.314 0.313 0.313 0.487 0.478 0.474 0.31 0.2 0.49 1.4

C16:0 9.36 9.25 9.24 11.7 12.0 11.6 9.4 0.7 11.8 1.8

C17:0 0.305 0.304 0.306 0.317 0.314 0.319 0.31 0.3 0.32 0.8

C18:0 3.11 3.09 3.10 1.92 1.92 1.92 3.11 0.3 1.92 0.0

C20:0 0.090 0.086 0.093 0.13 0.131 0.132 0.09 3.9 0.13 0.8

C16:1(n-7) 13.3 13.3 13.3 9.31 9.19 9.06 13.3 0.0 9.19 1.4

C18:1(n-7) 7.87 7.85 7.85 2.75 2.75 2.72 7.86 0.1 2.74 0.6

C18:1(n-9) 23.2 23.5 23.8 22.5 22.8 21.3 23.2 1.3 22.2 3.6

C18:1(n-9) <0.01 <0.01 <0.01 <0.01 <0.01 <0.01 NA NA NA NA

C20:1(n-7) 2.94 3.04 3.00 0.341 0.346 0.359 2.99 1.7 0.35 2.7

C20:1(n-9) 4.41 4.38 4.42 6.93 6.93 7.06 4.40 0.5 6.97 1.1

C:20:1(n-11) 2.96 2.94 2.98 4.49 4.55 4.51 2.96 0.7 4.49 0.7

C22:1(n-9) 0.379 0.372 0.376 0.764 0.758 0.782 0.38 0.9 0.77 1.6

C22:1(n-11) 0.782 0.779 0.781 6.68 6.77 7.02 0.78 0.2 6.82 2.6

C24:1(n-9) 0.0676 0.0635 0.064 0.289 0.292 0.318 0.07 3.3 0.30 5.3

C18:2(n-6) 0.946 0.939 0.942 1.18 1.17 1.20 0.94 0.4 1.18 1.3

C18:3(n-3) 0.105 0.103 0.104 0.421 0.424 0.429 0.10 1.0 0.42 1.0

C18:3(n-6) <0.01 <0.01 <0.01 <0.01 <0.01 <0.01 NA NA NA NA

C18:4(n-3) 0.190 0.190 0.190 0.324 0.331 0.333 0.19 0.0 0.33 1.4

C20:2(n-6) 0.330 0.351 0.291 0.261 0.259 0.267 0.32 9.4 0.26 1.6

C20:3(n-3) 0.036 0.042 0.04 0.194 0.187 0.188 0.04 7.8 0.19 2.0

C20:4(n-6) 0.454 0.448 0.451 0.322 0.332 0.333 0.45 0.7 0.33 1.8

C20:5(n-3) 2.38 2.42 2.38 1.64 1.70 1.68 2.39 1.0 1.67 1.8

C22:2(n-6) <0.01 <0.01 <0.01 <0.01 <0.01 <0.01 NA NA NA NA

C22:5(n-3) 6.89 6.84 6.86 1.25 1.25 1.34 6.86 0.4 1.28 4.1

C22:6(n-3) 6.02 5.99 6.00 4.94 5.05 5.58 6.00 0.3 5.19 6.6

Data as Submitted by Laboratory (fraction of total fatty acids (%))

Homogenate VI SRM 1945 Homog. VI SRM 1945
Lab F

FATTY ACID 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab 

%RSD %RSD

C12:0 0.061 0.101 0.091 0.410 0.444 0.410 0.06 34.2 0.42 4.6

C14:0 3.05 2.91 3.14 5.94 5.46 5.98 3.05 3.8 5.79 5.0

C15:0 0.342 0.385 0.337 0.600 0.537 0.565 0.34 7.8 0.60 5.2

C16:0 9.47 10.6 9.70 10.8 12.5 11.2 9.47 6.6 11.5 7.5

C17:0 0.376 0.283 0.344 0.417 0.303 0.387 0.38 12.6 0.37 16.0

C18:0 3.87 3.35 4.22 2.60 2.32 2.39 3.87 11.4 2.44 6.1

C20:0 NA NA NA NA NA NA NA NA NA NA

C16:1(n-7) 12.4 13.7 13.4 8.89 10.3 9.05 12.4 5.5 9.42 8.3

C18:1(n-7) 10.5 9.95 9.09 4.50 4.23 4.03 9.83 7.0 4.25 5.6

C18:1(n-9) 24.1 24.2 26.1 21.3 20.6 21.8 24.1 4.6 21.3 2.8

C18:1(n-9) NA NA NA NA NA NA NA NA NA NA

C20:1(n-7) NA NA NA NA NA NA NA NA NA NA

C20:1(n-9) 6.19 6.48 5.65 8.17 8.27 8.04 6.11 6.9 8.16 1.4

C:20:1(n-11) 2.80 2.58 2.57 5.32 5.16 5.40 2.80 4.7 5.32 2.3

C22:1(n-9) 0.451 0.366 0.350 1.40 1.42 1.41 0.39 13.9 1.41 0.8

C22:1(n-11) 0.904 0.778 0.787 7.26 8.16 7.59 0.82 8.5 7.67 5.9

C24:1(n-9) NA NA NA NA NA NA NA NA NA NA

C18:2(n-6) NA NA NA NA NA NA NA NA NA NA

C18:3(n-3) NA NA NA NA NA NA NA NA NA NA

C18:3(n-6) NA NA NA NA NA NA NA NA NA NA

C18:4(n-3) NA NA NA NA NA NA NA NA NA NA

C20:2(n-6) NA NA NA NA NA NA NA NA NA NA

C20:3(n-3) NA NA NA NA NA NA NA NA NA NA

C20:4(n-6) NA NA NA NA NA NA NA NA NA NA

C20:5(n-3) 2.20 2.17 2.30 1.69 1.41 1.76 2.22 3.0 1.62 11.6

C22:2(n-6) NA NA NA NA NA NA NA NA NA NA

C22:5(n-3) 6.78 6.55 5.17 1.81 1.53 1.88 6.17 14.1 1.74 10.6

C22:6(n-3) 4.15 3.77 3.89 3.92 3.49 3.90 3.94 4.9 3.77 6.5

Lipid (mass fraction (%)) 72.0 78.0 54.3 73.6 62.84 74.1 68.1 18.06 70.2 9.06

Data as Submitted by Laboratory (fraction of total fatty acids (%))

Homogenate VI SRM 1945 Homog. VI SRM 1945
Graphical results of PCB congener and lipid data reported by all 

laboratories. The z-scores for Homogenate VI represent 25% of the assigned 

value so that z = +1 is the assigned value plus 25 %, z = -1 is the assigned 

value minus 25 % and so forth. Error bars are ± 1 standard deviation.

Appendix B
0

2

4

6

8

10

12

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

0

1

2

3

4

5

6

7

8

9

10

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 18

Assigned value = <5 ng/g (wet basis)

Reported Results: 3 Quantitative Results: 3

Homogenate VI (MMQAVI)

PCB 18

Value = 4.48 ng/g ± 0.88 ng/g (wet basis)

Reported Results: 9 

SRM 1945
0

2

4

6

8

10

12

14

16

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

0

5

10

15

20

25

30

35

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 28

Assigned value = <5 ng/g (wet basis)

Reported Results: 10 Quantitative Results: 10

Homogenate VI (MMQAVI)

PCB 28

Value = 14.1 ng/g ± 1.4 ng/g (wet basis)

Reported Results: 16 

SRM 1945
0

0.5

1

1.5

2

2.5

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

0

2

4

6

8

10

12

14

16

18

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 31

Assigned value = <5 ng/g (wet basis)

Reported Results: 3 Quantitative Results: 3

Homogenate VI (MMQAVI)

PCB 31

Value = 3.12 ng/g ± 0.69 ng/g (wet basis)

Reported Results: 8 

SRM 1945
0

5

10

15

20

25

30

35

40

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

0

5

10

15

20

25

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 44

Assigned value = <5 ng/g (wet basis)

Reported Results: 9 Quantitative Results: 7

Homogenate VI (MMQAVI)

PCB 44

Value = 12.2 ng/g ± 1.4 ng/g (wet basis)

Reported Results: 13 

SRM 1945
0

5

10

15

20

25

30

35

40

45

50

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

0

5

10

15

20

25

30

35

40

45

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 49

Assigned value = <5 ng/g (wet basis)

Reported Results: 10 Quantitative Results: 9

Homogenate VI (MMQAVI)

PCB 49

Value = 20.8 ng/g ± 2.8 ng/g (wet basis)

Reported Results: 13 

SRM 1945
0

5

10

15

20

25

30

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

20

40

60

80

100

120

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 52

Assigned value = 3.78 ng/g s = 5.8 ng/g 95% CL = ± 3.0 ng/g (wet basis)

Reported Results: 16 Quantitative Results: 14

Homogenate VI (MMQAVI)

PCB 52

Value = 43.6 ng/g ± 2.5 ng/g (wet basis)

Reported Results: 18 

SRM 1945
0

2

4

6

8

10

12

14

16

18

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

10

20

30

40

50

60

70

80

90

100

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 66/95

Assigned value = 3.44 ng/g s = 2.8 ng/g 95% CL = ± 2.7 ng/g (wet basis)

Reported Results: 5 Quantitative Results: 4

Homogenate VI (MMQAVI)

PCB 66/95

Value = 57.4 ng/g (wet basis)

Reported Results: 6 

SRM 1945
0

5

10

15

20

25

30

35

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

5

10

15

20

25

30

35

40

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 87

Assigned value = 6.02 ng/g s = 1.3 ng/g 95% CL = 1.2 ± ng/g (wet basis)

Reported Results: 6 Quantitative Results: 5

Homogenate VI (MMQAVI)

PCB 87

Value = 16.7 ng/g ± 1.4 ng/g (wet basis)

Reported Results: 12 

SRM 1945
0

100

200

300

400

500

600

700

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

50

100

150

200

250

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 99

Assigned value = 353 ng/g s = 54 ng/g 95% CL = ± 30 ng/g (wet basis)

Reported Results: 14 Quantitative Results: 13

Homogenate VI (MMQAVI)

PCB 99

Value = 45.4 ng/g ± 5.4 ng/g (wet basis)

Reported Results: 14 

SRM 1945
0

5

10

15

20

25

30

35

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

20

40

60

80

100

120

140

160

180

200

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 101 (+90)

Assigned value = 8.49 ng/g s = 7.5 ng/g 95% CL = ± 3.8 ng/g (wet basis)

Reported Results: 17 Quantitative Results: 15

Homogenate VI (MMQAVI)

PCB 101 (+90)

Value = 65.2 ng/g ± 5.6 ng/g (wet basis)

Reported Results: 18 

SRM 1945
0

5

10

15

20

25

30

35

40

45

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

10

20

30

40

50

60

70

80

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 105

Assigned value = 18.0 ng/g s = 7.4 ng/g 95% CL = ± 3.6 ng/g (wet basis)

Reported Results: 17 Quantitative Results: 16

Homogenate VI (MMQAVI)

PCB 105

Value = 30.1 ng/g ± 2.3 ng/g (wet basis)

Reported Results: 18 

SRM 1945
0

20

40

60

80

100

120

140

160

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

50

100

150

200

250

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 118

Assigned value = 58.8 ng/g s = 16 ng/g 95% CL = ± 7.5 ng/g (wet basis)

Reported Results: 19 Quantitative Results: 17

Homogenate VI (MMQAVI)

PCB 118

Value = 74.6 ng/g ± 5.1 ng/g (wet basis)

Reported Results: 19 

SRM 1945
0

5

10

15

20

25

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

10

20

30

40

50

60

70

80

90

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 128

Assigned value = 12.0 ng/g s = 2.3 ng/g 95% CL = ± 1.2 ng/g (wet basis)

Reported Results: 15 Quantitative Results: 15

Homogenate VI (MMQAVI)

PCB 128

Value = 23.7 ng/g ± 1.7 ng/g (wet basis)

Reported Results: 15 

SRM 1945
0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

5

10

15

20

25

30

35

40

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 132

Assigned value = <8 ng/g (wet basis)

Reported Results: 0 Quantitative Results: 0

Homogenate VI (MMQAVI)

PCB 132

Value = 22.5 ng/g ± 4.6 ng/g (wet basis)

Reported Results: 9 

SRM 1945
0

100

200

300

400

500

600

700

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

50

100

150

200

250

300

350

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 138 (+163+164)

Assigned value = 229 ng/g s = 82 ng/g 95% CL = ± 39 ng/g (wet basis)

Reported Results: 17 Quantitative Results: 17

Homogenate VI (MMQAVI)

PCB 138 (+163+164)

Value = 132 ng/g ± 7.4 ng/g (wet basis)

Reported Results: 17 

SRM 1945
0

1

2

3

4

5

6

7

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

20

40

60

80

100

120

140

160

180

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 149

Assigned value = 2.66 ng/g s = 0.90 ng/g 95% CL = ± 0.59 ng/g (wet basis)

Reported Results: 11 Quantitative Results: 9

Homogenate VI (MMQAVI)

PCB 149

Value = 107 ng/g ± 8.4 ng/g (wet basis)

Reported Results: 15 

SRM 1945
0

10

20

30

40

50

60

70

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

10

20

30

40

50

60

70

80

90

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 151

Assigned value = <8 ng/g (wet basis)

Reported Results: 8 Quantitative Results: 7

Homogenate VI (MMQAVI)

PCB 151

Value = 28.7 ng/g ± 5.2 ng/g (wet basis)

Reported Results: 13 

SRM 1945
0

500

1000

1500

2000

2500

3000

3500

4000

4500

5000

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

50

100

150

200

250

300

350

400

450

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 153

Assigned value = 1607 ng/g s = 522 ng/g 95% CL = ± 256 ng/g (wet basis)

Reported Results: 17 Quantitative Results: 16

Homogenate VI (MMQAVI)

PCB 153

Value = 213 ng/g ± 19 ng/g (wet basis)

Reported Results: 17 

SRM 1945
0

20

40

60

80

100

120

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

10

20

30

40

50

60

70

80

90

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 156

Assigned value = 60 ng/g s = 16 ng/g 95% CL = ± 8 ng/g (wet basis)

Reported Results: 16 Quantitative Results: 16

Homogenate VI (MMQAVI)

PCB 156

Value = 10 ng/g ± 1 ng/g (wet basis)

Reported Results: 15 

SRM 1945
0

100

200

300

400

500

600

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

20

40

60

80

100

120

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 170 (+190)

Assigned value = 309 ng/g s = 119 ng/g 95% CL = ± 53 ng/g (wet basis)

Reported Results: 19 Quantitative Results: 19

Homogenate VI (MMQAVI)

PCB 170 (+190)

Value = 40.6 ± 2.6 ng/g (wet basis)

Reported Results: 19 

SRM 1945
0

200

400

600

800

1000

1200

1400

1600

1800

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

50

100

150

200

250

300

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 180

Assigned value = 653 ng/g s = 185 ng/g 95% CL = ± 88 ng/g (wet basis)

Reported Results: 18 Quantitative Results: 17

Homogenate VI (MMQAVI)

PCB 180

Value = 107 ng/g ± 5.3 ng/g (wet basis)

Reported Results: 18 

SRM 1945
0

10

20

30

40

50

60

70

80

90

100

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

10

20

30

40

50

60

70

80

90

100

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 183

Assigned value = 24.3 ng/g s = 8.8 ng/g 95% CL = ± 4.8 ng/g (wet basis)

Reported Results: 15 Quantitative Results: 13

Homogenate VI (MMQAVI)

PCB 183

Value = 36.6 ng/g ± 4.1 ng/g (wet basis)

Reported Results: 16 

SRM 1945
0

5

10

15

20

25

30

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

50

100

150

200

250

300

350

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 187

Assigned value = 10.1 ng/g s = 5.0 ng/g 95% CL = ± 2.7 ng/g (wet basis)

Reported Results: 14 Quantitative Results: 13

Homogenate VI (MMQAVI)

PCB 187

Value = 105 ng/g ± 9.1 ng/g (wet basis)

Reported Results: 16 

SRM 1945
0

50

100

150

200

250

300

350

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

50

100

150

200

250

300

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 194

Assigned value = 163 ng/g s = 51 ng/g 95% CL = ± 24 ng/g (wet basis)

Reported Results: 17 Quantitative Results: 18

Homogenate VI (MMQAVI)

PCB 194

Value = 39.6 ng/g ± 2.5 ng/g (wet basis)

Reported Results: 17 

SRM 1945
0

2

4

6

8

10

12

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

10

20

30

40

50

60

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 195

Assigned value = 4.18 ng/g s = 2.0 ng/g 95% CL = ± 1.1 ng/g (wet basis)

Reported Results: 13 Quantitative Results: 12

Homogenate VI (MMQAVI)

PCB 195

Value = 17.7 ng/g ± 4.3 ng/g (wet basis)

Reported Results: 13 

SRM 1945
0

10

20

30

40

50

60

70

80

90

100

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

20

40

60

80

100

120

140

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 201

Assigned value = 6.45 ng/g s = 3.5 ng/g 95% CL = ± 2.6 ng/g (wet basis)

Reported Results: 8 Quantitative Results: 7

Homogenate VI (MMQAVI)

PCB 201

Value = 17.0 ng/g ± 0.89 ng/g (wet basis)

Reported Results: 10 

SRM 1945
0

10

20

30

40

50

60

70

80

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

20

40

60

80

100

120

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 206

Assigned value = 32.4 ng/g s = 6.4 ng/g 95% CL = ± 3.5 ng/g (wet basis)

Reported Results: 14 Quantitative Results: 13

Homogenate VI (MMQAVI)

PCB 206

Value = 31.1 ng/g ± 2.7 ng/g (wet basis)

Reported Results: 14 

SRM 1945
0

10

20

30

40

50

60

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

5

10

15

20

25

30

35

40

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 209

Assigned value = 12.3 ng/g s = 2.1 ng/g 95% CL = ± 1.1 ng/g (wet basis)

Reported Results: 15 Quantitative Results: 13

Homogenate VI (MMQAVI)

PCB 209

Value = 10.6 ng/g ± 1.1 ng/g (wet basis)

Reported Results: 15 

SRM 1945
0

10

20

30

40

50

60

70

80

90

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

0

5

10

15

20

25

30

35

40

45

50

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 66

Assigned value = <10 ng/g (wet basis)

Reported Results: 7 Quantitative Results: 6

Homogenate VI (MMQAVI)

PCB 66

Value = 23.6 ng/g ± 1.6 ng/g (wet basis)

Reported Results: 11 

SRM 1945
0

10

20

30

40

50

60

70

80

90

100

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

0

5

10

15

20

25

30

35

40

45

50

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

PCB 95

Assigned value = <10 ng/g (wet basis)

Reported Results: 7 Quantitative Results: 8

Homogenate VI (MMQAVI)

PCB 95

Value = 33.8 ng/g ± 1.7 ng/g (wet basis)

Reported Results: 11 

SRM 1945
Graphical results of pesticide and lipid data reported by all laboratories. The 

z-scores for Homogenate VI represent 25% of the assigned value so that z = 

+1 is the assigned value plus 25 %, z = -1 is the assigned value minus 25 % 

and so forth. Error bars are ± 1 standard deviation.

Appendix C
0

5

10

15

20

25

30

35

40

45

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

0

20

40

60

80

100

120

140

160

180

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

2,4’-DDT

Assigned value = <5 ng/g (wet basis)

Reported Results: 8 Quantitative Results: 8

Homogenate VI (MMQAVI)

2,4’-DDT

Value = 106 ng/g ± 14 ng/g (wet basis)

Reported Results: 16 

SRM 1945
0

10

20

30

40

50

60

70

80

90

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

100

200

300

400

500

600

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

4,4’-DDT

Assigned value = 8.83 ng/g s = 4.1 ng/g 95% CL = ± 2.2 ng/g (wet basis)

Reported Results: 16 Quantitative Results: 14

Homogenate VI (MMQAVI)

4,4’-DDT

Value = 245 ng/g ± 15 ng/g (wet basis)

Reported Results: 20 

SRM 1945
0

10

20

30

40

50

60

70

80

90

100

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

0

5

10

15

20

25

30

35

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

2,4’-DDE

Assigned value = <5 ng/g (wet basis)

Reported Results: 7 Quantitative Results: 3

Homogenate VI (MMQAVI)

2,4’-DDE

Value = 12.3 ng/g ± 0.87 ng/g (wet basis)

Reported Results: 12 

SRM 1945
0

20

40

60

80

100

120

140

160

180

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

100

200

300

400

500

600

700

800

900

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

4,4’-DDE

Assigned value = 72.3 ng/g s = 18 ng/g 95% CL = ± 8.0 ng/g (wet basis)

Reported Results: 21 Quantitative Results: 19

Homogenate VI (MMQAVI)

4,4’-DDE

Value = 445 ng/g ± 37 ng/g (wet basis)

Reported Results: 21 

SRM 1945
0

2

4

6

8

10

12

14

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

0

5

10

15

20

25

30

35

40

45

50

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

2,4’-DDD

Assigned value = <5 ng/g (wet basis)

Reported Results: 5 Quantitative Results: 4

Homogenate VI (MMQAVI)

2,4’-DDD

Value = 18.1 ng/g ± 2.8 ng/g (wet basis)

Reported Results: 13 

SRM 1945
0

2

4

6

8

10

12

14

16

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

50

100

150

200

250

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

4,4’-DDD

Assigned value = 3.71 ng/g s = 3.3 ng/g 95% CL = ± 1.6 ng/g (wet basis)

Reported Results: 18 Quantitative Results: 16

Homogenate VI (MMQAVI)

4,4’-DDD

Value = 133 ng/g ± 10 ng/g (wet basis)

Reported Results: 20 

SRM 1945
0

50

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350

400

450

500

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

5

10

15

20

25

30

35

40

45

50

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

HCB

Assigned value = 242 ng/g s = 75 ng/g 95% CL = ± 36 ng/g (wet basis)

s

Homogenate VI (MMQAVI)

HCB

Value = 32.9 ng/g ± 1.7 ng/g (wet basis)

Reported Results: 18 

SRM 1945
0

20

40

60

80

100

120

140

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

5

10

15

20

25

30

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

alpha-HCH

Assigned value = 65.4 ng/g s = 21 ng/g 95% CL = ± 11 ng/g (wet basis)

Reported Results: 17 Quantitative Results: 14

Homogenate VI (MMQAVI)

alpha-HCH

Value = 16.2 ng/g ± 3.4 ng/g (wet basis)

Reported Results: 17 

SRM 1945
0

500

1000

1500

2000

2500

3000

3500

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

5

10

15

20

25

30

35

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

beta-HCH

Assigned value = 1102 ng/g s = 375 ng/g 95% CL = ± 184 ng/g (wet basis)

Reported Results: 18 Quantitative Results: 16

Homogenate VI (MMQAVI)

beta-HCH

Value = 8.0 ng/g ± 1.4 ng/g (wet basis)

Reported Results: 13 

SRM 1945
0

1

2

3

4

5

6

7

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

0

5

10

15

20

25

30

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

gamma-HCH

Assigned value = <5 ng/g (wet basis)

Reported Results: 13 Quantitative Results: 12

Homogenate VI (MMQAVI)

gamma-HCH

Value = 3.3 ng/g ± 0.81 ng/g (wet basis)

Reported Results: 14 

SRM 1945
0

20

40

60

80

100

120

140

160

180

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

5

10

15

20

25

30

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

Heptachlor Epoxide

Assigned value = 95.5 ng/g s = 28 ng/g 95% CL = ± 15 ng/g (wet basis)

Reported Results: 14 Quantitative Results: 13

Homogenate VI (MMQAVI)

Heptachlor Epoxide

Value = 10.8 ng/g ± 1.3 ng/g (wet basis)

Reported Results: 13 

SRM 1945
0

20

40

60

80

100

120

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

20

40

60

80

100

120

140

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

Cis-Chlordane

Assigned value = 22.1 ng/g s = 40 ng/g 95% CL = ± 29 ng/g (wet basis)

Reported Results: 8 Quantitative Results: 7

Homogenate VI (MMQAVI)

Cis-Chlordane

Value = 46.9 ng/g ± 2.8 ng/g (wet basis)

Reported Results: 17 

SRM 1945
0

10

20

30

40

50

60

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

10

20

30

40

50

60

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

Trans-Chlordane

Assigned value = 6.0 ng/g s = 14 ng/g 95% CL = ± 10 ng/g (wet basis)

Reported Results: 10 Quantitative Results: 8

Homogenate VI (MMQAVI)

Trans-Chlordane

Value = 12.1 ng/g ± 1.14 ng/g (wet basis)

Reported Results: 13 

SRM 1945
0

200

400

600

800

1000

1200

1400

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

5

10

15

20

25

30

35

40

45

50

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

Oxychlordane

Assigned value = 534 ng/g s = 215 ng/g 95% CL = ± 113 ng/g (wet basis)

Reported Results: 16 Quantitative Results: 14

Homogenate VI (MMQAVI)

Oxychlordane

Value = 19.8 ng/g ± 1.9 ng/g (wet basis)

Reported Results: 16 

SRM 1945
0

1

2

3

4

5

6

7

8

9

10

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

10

20

30

40

50

60

70

80

90

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

Cis-Nonachlor

Assigned value = 5.25 ng/g s = 1.4 ng/g 95% CL = ± 0.84 ng/g (wet basis)

Reported Results: 12 Quantitative Results: 11

Homogenate VI (MMQAVI)

Cis-Nonachlor

Value = 48.7 ng/g ± 7.6 ng/g (wet basis)

Reported Results: 14 

SRM 1945
0

50

100

150

200

250

300

350

400

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

50

100

150

200

250

300

350

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

Trans-Nonachlor

Assigned value = 157 ng/g s = 34 ng/g 95% CL = ± 19 ng/g (wet basis)

Reported Results: 15 Quantitative Results: 12

Homogenate VI (MMQAVI)

Trans-Nonachlor

Value = 231 ng/g ± 11 ng/g (wet basis)

Reported Results: 15 

SRM 1945
0

50

100

150

200

250

300

350

400

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

10

20

30

40

50

60

70

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

Dieldrin

Assigned value = 214 ng/g s = 49 ng/g 95% CL = ± 27 ng/g (wet basis)

Reported Results: 14 Quantitative Results: 12

Homogenate VI (MMQAVI)

Dieldrin

Value = 37.5 ng/g ± 3.9 ng/g (wet basis)

Reported Results: 13 

SRM 1945
0

10

20

30

40

50

60

70

80

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

10

20

30

40

50

60

70

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

Mirex

Assigned value = 11.0 ng/g s = 4.2 ng/g 95% CL = ± 2.5 ng/g (wet basis)

Reported Results: 13 Quantitative Results: 11

Homogenate VI (MMQAVI)

Mirex

Value = 28.9 ng/g ± 2.8 ng/g (wet basis)

Reported Results: 14 

SRM 1945
0

20

40

60

80

100

120

140

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

0

20

40

60

80

100

120

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value

Lipid

Assigned value = 73.1 % s = 4.3 % 95% CL = ± 1.9 % (mass fraction)

Reported Results: 21 Quantitative Results: 20

Homogenate VI (MMQAVI)

Lipid

Value = 74.3 % ± 0.45 % (mass fraction)

Reported Results: 21 

SRM 1945
Tabular summary of methods used for analysis by each laboratory

Appendix D
Summary of Methods Used

Laboratory Procedure used to measure Lipid

SRM 1945 Homog. VI SRM 1945 Homog. VI

1 1 1

Subsampled the dichloromethane extract, evaporated the

solvent and weighed the remaining residue. wet wet

2 1 1

PFE hexane:acetone 4:1; repeated extraction, combined 

extracts; repeat drying 30 min @ 100-101° C until 

plateau. wet wet

3 1 1

Gravimetric. After extraction, a 5 mL aliquote of a 100

mL volume is dried to determine lipid percent. wet wet

4 1 1

Gravimetric; ASE extraction using dichloromethane,

followed by evaporation of solvent. Lipid determined as

total extractable organics. wet wet

5 0.29 0.31

Pentane/hexane extraction and quantitation by thin-layer

chromatagraphy with flame ionization detection

(TLC/FID). wet wet

6 1.6 to 2.9 4.0 to 5.8

After extraction with dichloromethane, each extract was

concentrated to 10 mL. 2 mL of this extract was added to

a previously weighed and dried aluminum pan. The

contents were allowed to evaporate, and the pan was

reweighed. dry dry

7 1.37 to 1.40 1.31-1.50 Gravimetric, 10% of extract. wet wet

8 0.3 0.2

Determined % lipid in the process of sample extraction,

i.e., did not use separate sample for lipid. Mortar pestle /

sodium sulfate / gravity column extraction with 1:1

DCM:HEX collected extract / evaporated by roto vap and

nitrogen . dry dry

9 1 1 Gravimetric wet wet

10 0.9 to 1 g 0.8 to 1 g

Removed 1mL aliquot from 10 mL sample extract and

placed in small preweighed glass vial. Evaporated DCM

in the hood for a total of 24 hours. Weighed individual

vials after 12 and 24 hours. Averaged the masses and

calculated a percent lipid (n=3 for each sample). wet wet

11 0.9 0.9 An aliquot of total extract was dried and weighed wet wet

12 2 2

Sample extracted with ethyl ether/petroleum ether (1:1).

Evaporate the solvent and record the weight that stays in

container. wet wet

Approximate mass of sample 

extracted (g):

Were "wet" or "dry" samples 

extracted?
Summary of Methods Used

Laboratory Procedure used to measure Lipid

SRM 1945 Homog. VI SRM 1945 Homog. VI

13 2 2 Gravametric on whole sample wet wet

14 1 1 *NA wet wet

15 0.55 0.51

Dionex PFE (ASE) method # 342. Preheat 1 min, heat 6

min, static 3 min, methylene chloride, oven temp 125 °C

2000 psi, 3 cycles, 60 % vol. flush, 120 sec. purge.

Samples extracted for lipids, evaporated and extracts

dried at 70 °C. wet wet

16 1.5 to 2.5 1.5 to 2.5

Gravimetric determination after extraction with

cyclohexsane and acetone. The lipide xtract was dried at

least 24 hours at 40 °C in a sand bath then weighted untill

constant weight. wet wet

17 2.2 3.1

It was determined gravimetrically from an aliquot of the 

Soxhlet extraction. wet wet

18 0.27 0.3

Samples were mixed with sodium sulphate and Soxhlet

extracted. Lipids were determined on an aliquot of the

extract (~1/6) after solvent evaporation at 105 °C for 1h. wet wet

19 3 2.1 Gravimetrc analysis wet wet

20 5.1 5.5

see Smedes, Foppe. Determination of total lipid using

non-chlorinated solvents. Analyst (Cambridge, United

Kingdom) (1999), 124(11), 1711-1718 wet wet

21 2 2 *NA wet wet

22 1.04 to 1.8 1.6 to 2.1

Gravimetric determination from portion of the Soxhlet 

extraction. wet wet

23 0.9 1.0 weigh aliquat of extract wet wet

A 1 1

Gravimetric; PFE extraction using dichloromethane,

followed by evaporation of solvent. Lipid determined as

total extractable organics. wet wet

B NA NA NA NA NA

C 1.2 0.8

ASE hexane:acetone 4:1, eepeated extraction, combined

extracts; Repeat drying 30 min @ 100 ° C to 101 ° C until

plateau. wet wet

D NA NA NA NA NA

E 1 1

Sample extraction: tissue was macerated with anhydrous 

sodium sulfate and extracted with hexane using sonication

at room temperature. wet wet

F 2.6 3.9

NOAA Sampling an Analytical Method of the National

Status and Trends Program NOAA Technical

Memorandum NOS ORCA 130. wet wet

*NA Not applicable or data not reported

Approximate mass of sample 

extracted (g):

Were "wet" or "dry" samples 

extracted?
Summary of Methods Used

Laboratory Extraction Method Extraction Solvent Extraction Time Extraction Other

Sample Extract Cleanup 

Method

1

Pressurized Fluid 

Extraction (Dionex, 

ASE) Dichloromethane 15 min

Sample dried with 45 g

sodium sulfate, then packed

into a 33 mL PFE cell. PFE

Conditions: cell temp 100

°C. equilibration 5 min,

static time 5 min, cell

pressure 2000 psi and there

were three cylces. Samples

were first run through a Gel

Permeation chrom

Samples were first run through a

Gel Permeation

chromatogramphy column (PL

Gel 600 mm) using DCM as the

mobile phase. Eluent containing

orgaonochlorines was cleaned up

by automated SPE using a 1 g

Florisil column then fractionated

on a silicic acid colum

2

Pressurized Fluid 

Extraction (Dionex, 

ASE) Dichloromethane 15 min 100 °C; 2000 psi, 3 cycles; 90 s

SEC - collect 70 mL fraction;

conc with solvent change to

hexane NH2 Sep Pak - condition

and elute with 15 mL of 10%

dichloromethane in hexane

concentrate for GC/MS.

3

Pressurized Fluid 

Extraction (Dionex, 

ASE) Dichloromethane 40 min

A 1 gram sample is extracted

using a Dionex Accelerated

Solvent Extractor at 110 degrees

Celsius and 1300 psi. The solvent

is reduced and the oil is removed

by size exclusion

chromatography using SX-3 Biobeads. The sample is then passed

through a florisil 1 gram

disposable cartridge on a Zymark

Rapid Trace SPE workstation.

4

Pressurized Fluid 

Extraction (Dionex, 

ASE) Dichloromethane 16 min

ASE Conditions: Pressure =

2000 psi, temp = 100 °C

Gravity flow column with silca

and neutral alumina, followed by

HPLC-SEC to elute AH/CH

fraction.

5

See Krahn et al. 1994 

Chemosphere

Pentane/hexane 

(50:50; v/v) 4 min

Acidic/basic/neutral silica gel

column (see Krahn et al. 1994

Chemosphere).

6 Soxhlet extraction dichloromethane 18 h

After lipid determination was

completed, each extract was

eluted through a glass

chromatography column packed

with florisil, alumina, and silica

and was concentrated to 10mL.

Two 1 mL portions of this eluate

were each diluted to 5 mL with

methylene chlo

7 Ball Mill Hexane 2 x 10 min

Florisil (1.2% deactivated)

fractionation and clean up for OC

Pesticides, PCBs F1-hexane, F2-

15/85 DCM/Hexane, F3-50/50

DCM/Hexane.
8

1 : 1 DCM : 

HEXANE 

Sample was weighed,

spiked with internal stds.,

pulverized with 50 g of

anhydrous sodium sulfate

using a mortar/pestle,

transferred quantitatively to

glass column,1:1

DCM:HEX was added 325

mL of solvent percolated

through column (ie gravity

column extract

PCBs: Single column of layers;

from top (neutral silica / acidic

silica / neutral silica / basic silica)-

( sample added in 1:1 DCM :

HEXANE and eluted with same)

Further cleanup on neutral

alumina ( sample added in

hexane, hexane added to column,

discar

9 Soxhlet Dichloromethane 12 h GPC, activated silica

10

Pressurized Fluid 

Extraction (Dionex, 

ASE) Dichloromethane 1 h 45 min

Gel Permeation Chromatography

(GPC) with Biobeads (SX-3)

followed by florisil column

chromatography GPC-- F1 100

ml 50 % DCM in Hexane

(discard); F2 200 ml 50 % DCM

in Hexane. Evaporate to 1 ml for

florisil. Florisil-- 18 g deactivated

florisil (1%) wash

11

Pressurized Fluid 

Extraction (Dionex, 

ASE) Dichloromethane 5 min x 3 NaSO4

Silica gel column followed by

GPC
Summary of Methods Used

Laboratory Extraction Method Extraction Solvent Extraction Time Extraction Other

Sample Extract Cleanup 

Method

12

Sample mixed with

anhydrous sodium

sulfate and extracted

three times with organic

solvent

ethyl 

ether/petroleum 

ether (1:1) 1 h

Silica gel 60 column clean up.

Elute with hexane first followed

by benzene.

13 ASE Dichloromethane 11 minues

1/10 of the extracted fat was 

used to go through cleanup 

and analysis.

Preheat 0; Heat 5 min; Static 2

min; Flush 100%; Purge 30 sec;

Cycles 2; Pressure 1500 psi;

Temp 100C; Cell size 22 ml

14 Liquid/Liquid Pentane 15 min

Florisil SPE, sodium sulfate,

extract with

dichoromethane/hexane.

15

Tissue lightly ground 

with sodium sulfate, wet 

packed in hexane in a 

35cm long x 3.1cm ID 

column with 300ml 

reservoir.

Dichloromethane: 

hexane 1:1 30 min 100 o

C, 2000 psi, 3 cycles

Extract evaporated to 8 mL and

injected on gel permeation

chromatograph to remove lipids,

using 1:1

dichloromethane:hexane. 

Extract evaporated to 1 mL and

separated on a 8 g activated silica

gel column. 30 cm long column x

1 cm ID, with 250 mL reservoir

16 Ultrasonication

Cyclohexane and 

acetone 3 min Sulfuric acid

17 Soxhlet Hexane 5 h Mixed with sulfuric acid Sulfuric acid

18 Hot Soxhlet

Hexane:Acetone=3:

1 (v/v) 2 h

Column chromatography on

silica/acid silica elution with 15

ml hexane and 10 ml

dichloromethane.

19 Soxhlet

dichloromethane & 

hexane (3:1) 16 h

Sulfuric acid treatment followed

by Florisil cleanup.

20 Dialysis 300 mL hexane 60 h

(1) Multilayer: Silica gel // Silica

gel-H2SO4 (44 %, w:w) // Silica

gel-H2SO4 (22 %, w:w); (2)

Multilayer: Silica gel // Silica gelKOH // Silica gel-H2SO4 (44 %,

w.w); and (3) Fractionation:

ENVI-Carb SPE.

21

5% ethanol in ethyl 

acetate, 50 mL

homogenize for 2 

min

25 g anyhydrous sodium sulfate +

50 mL 5 % ethanol in ethyl

acetate were added to each

sample. Following

homogenization, samples were

centrifuged and the supernatant

was pulled off. A silica SPE

cartridge was used for sample

cleanup. Following SPE

extraction, the samples were

evaporated under a stream of

nitrogen, and then reconstituted

with 1mL of toluene.
22 Soxhlet

Mixture of hexane: 

diethylether (1:3) 8 h

Sample was ground with

anhydrous sodium sulfate. 

Silica-gel column

chromatography (activated by 3-

6hours at 130 o

C., 1.5 g, cloumn

i.d.: 10mm.) after gel permeation

chromatography (GPC) (Gel: Biobead S-X3, Column: 2 cm i.d. 50

cm length).

23 grinding with sodium suldichloromethane 3 x 3 min

Slica gel/alumina and phenogel;

aliquot of the extract was cleaned

up further in order to sparate the

planar PCBs from the other

anlytes on a charcoal column.

A

Accelerated solvent 

extraction (ASE) Dichloromethane ~16 min.

ASE Conditions: Pressure =

2000 psi, temp = 100 °C

B NA NA NA NA NA

C

Accelerated solvent 

extraction (ASE) Hexane: acetone 4:1

3 static cycles, 6 

minutes each

1500 psig, 125 C, 100%

flush.

Transesterification via NaOH and 

BF3/MeOH

D NA NA NA NA NA

E

Sonication with sodium 

sulfate at room 

temperature. Hexane

Derivatization: fatty acid methyl

esters (FAMEs) were prepared

using methanolic KOH at room

temperature. (Christopherson, S.

W. and Glass, R. L., 1969.

Preparation of milk fat methyl

esters by alcoholysis in an

essentially nonalcoholic solution.

J. Dairy

F

Modfied NOAA Status 

and Trends for 

Extraction of Biological 

Tissues for Trace 

Organics.

dichloromethane:M

EOH (3:1 v/v)- 0.01 

%BHT added as 

preservative

10 minutes 

sonication

Florisil column used post

derivatization.
Summary of Methods Used

Laboratory Instrument Column Phase

Length 

(m)

Column i.d. 

(mm) Film Thickness (μm) Points Conc. Range

Ana

Cal

1 Pest. GC-MS DB-5ms 60 0.25 0.25 5 2-1000 ng/mL 

PCB GC-MS DB-5ms 60 0.25 0.25 5 2-1000 ng/mL 

2 Pest. GC/MS EI DB-17 60 0.25 0.25 6 10 ng/g to 500 ng/g

PCB DB-17 60 0.25 0.25 6 10 ng/g to 500 ng/g

PCB 1

3 Pest. GC-MS (SIM) DB-5 30 0.25 0.25 5 0.2-15 ng/μL

PCB GC-MS (SIM) DB-5 30 0.25 0.25 5 0.05-15 ng/μL

4 Pest. GC-MS DB-5 60 0.25 0.25 5 to 8 0.003-10 ng/μL 

PCB GC-MS DB-5 60 0.25 0.25 5 to 8 0.003-10 ng/μL

CB 19

5 Pest. HPLC/PDA

2-(5-PYE) 

analytical 

columns, in 

series 0.25 4.6 5 μm particles 1 0.322 ng/μL

PCB HPLC/PDA

2-(5-PYE) 

analytical 

columns, in 

series 0.25 4.6 5 μm particles 1 0.322 ng/μL

6 Pest. GC-ECD DB-5 60 0.25 0.25 1 100 ng/ml

4,4’

1

PCB GC-ECD DB-5 60 0.25 0.25 1 100 ng/ml

PCB 1

analy

170, & 

7 Pest. GC-ECD DB-5 60 0.25 0.25 5-80 ng/μL

PCB GC-ECD DB-5 60 0.25 0.25

8 Pest. VG Autospec DB 5 54.3 0.25 0.1 5 2 pg/ul to 500 pg/μL 

PCB

VG Autospec/ 

Micromass 

Ultima(HRGCHRMS) Electron 

Impact Ionization DB-5 60 0.25 0.1 4 0.5 pg/ul to 200 pg/μL 

9 Pest. GC-ECD HP-5 30 0.25 0.25 4 5-100 pg/«L

PCB GC-ECD HP-5 30 0.25 0.25 4 0.6-120 pg/μL

10 Pest.

GC-ECD/GCNCIMS DB-XLB 30 0.25 0.25 6 2.6-106 ng/ml 

PCB

GC-ECD/GCNCIMS DB-XLB 30 0.25 0.25 6 2.4 - 97 ng/ml 

11 Pest. GC/ECD DB-XLB; DB-5 60; 60 0.25 ;0.25 0.25 ;0.25 5 2.5 - 200 ng/mL 

PCB GC/ECD DB-XLB; DB-5 60; 60 0.25; 0.25 0.25; 0.25 5 2.5 - 200 ng/mL 
Summary of Methods Used

Laboratory Instrument Column Phase

Length 

(m)

Column i.d. 

(mm) Film Thickness (μm) Points Conc. Range

Ana

Cal

12 Pest. Varian 3400 15 0.32 0.25 6 1.00 to 500 ng/mL

PCB

13 Pest. GC-ECD DB-608 30 0.53 0.83 3 2 to 250 pg/μL 

Pest. GC-ECD DB-5 30 0.53 1.5

PCB GC-ECD DB-XLB 60 0.25 0.25 5 1 to 200 pg/μL 

14 Pest. GC-ECD

50% 

PHMESiloxane 30 0.25 0.25 4 10-250 ppb, 250-1000 ppb 

PCB GC-ECD

50% 

PHMESiloxane 30 0.25 0.25 4 50-500 ppb 

15 Pest. GC/uECD

5% Ph Me 

Siloxane 60 0.25 0.25 5 0.9-155 pg/μL

For 

PCB GC/uECD

5% Ph Me 

Siloxane 60 0.25 0.25 5 1.5-203.5 pg/μL

For Ho

99, 1

16 Pest. GC-ECD

SPB-5 and SPB1701 (supelco 

Inc) 60 0.25 0.25 5

0.48-104 ng/mL (varies for the 

different compounds)

PCB GC-ECD

SPB-5 and SPB1701 (supelco 

Inc) 60 0.25 0.25 5

0.63-100 ng/mL (varies for the 

different compounds)

17 Pest. Hewlet Packard 3365 SPB-5 60 0.25 25 2

PCB Hewlet Packard 3365 SPB-5 60 0.25 25 2

18 Pest. GC/MS (ECNI) HT-8 25 0.22 0.25 8 

PCB GC/MS (EI) DB-1 30 0.25 0.25 8 

19 Pest. GC-ECD

Phenomenex 

ZB-5 30 0.25 0.25

PCB GC-MS J&W DB-1 30 0.25 0.25

20 Pest. GC-μECD DB-5 60 0.25 0.25 7 10-1000 pg/μL

PCB

21 Pest. GC-MS VF-5ms 30 0.25 0.25

PCB GC-MS VF-5ms 30 0.25 0.25

22 mono-hepta BDEs GC-EI-MSD DB-1 30 0.25 0.25 8 4-1000 ng/ml 

octa-deca BDEs GC-EI-MSD DB-1 15 0.25 0.10

23 Pest. GC-ECD DB 5 30 0.25 0.25 4 5 to 200 ng/μL 

PCB GC-ECD DB 5 30 0.25 0.25 4 5 to 200 ng/μL 

A Fatty Acid GC-MS (EI; SIM) DB-23 60 0.25 0.25 8 0.005-40 ng/μL (as FAMEs) 

B Fatty Acid

C Fatty Acid GC-FID SP2560 100 0.25 0.2D Fatty Acid

E Fatty Acid GC-MS/GC-FID HP-225 30 0.25 0.25

F Fatty Acid GC/MS-MS DB 5 30 0.25 0.25
Summary of Methods Used

Laboratory

Method of 

Quantitation Added Prior to Extraction 

Indentity of Internal Standards Added 

Prior to Chromatographic Analysis

1 Pest. IS Deuterated 4,4'-DDE, 4,4'-DDD, 4,4'-DDT, endosulfan I; 13C-cis chlordane

PCB IS 13C labeled PCBs 28, 52, 118, 153, 180, 194, 206, and 209

2 Pest. IS

Deuterated 4,4'-DDD and Carbon-13 labeled 4,4'-DDT, 4,4'-DDE, lindane, and transnonachlor

PCB IS

PCB 103, PCB 198, and Carbon-13 labeled PCB 28, PCB 101, PCB 105, PCB 153, and 

PCB 170

3 Pest. IS PCB 103,198 deuterated phenanthrene, chrysene

PCB IS PCB 103,198 deuterated phenanthrene, chrysene

4 Pest. IS CB 103 tetrachloro-o-xylene

PCB IS CB 103 tetrachloro-o-xylene

5 Pest. IS 1,2,3,4-tetrachloro-p -dibenzodioxin 1,7,8-trichloro-p -dibenzodioxin

PCB IS 1,2,3,4-tetrachloro-p -dibenzodioxin 1,7,8-trichloro-p -dibenzodioxin

6 Pest. Ronnel Tetrachloro-m-xylene (TCMX)

PCB IS 4,4' Dibromooctafluorobiphenyl (DOB), PCB 198 PCB 103. 

7 Pest. IS

PCB IS

8 Pest. IS

13C6 Chlorobenzene; 13C6 1, 4 Dichlorobenzene; 13C6 1,2,3 Trichlorobenzene; 13C6 1234 

Tetrachlorobenzene; 13C6 Pentachlorobenzene; 13C6 Hexachlorobenzene; 13C6 Lindane; 13C12 p,p'-DDE; 13C12 p,p'-DDT; d4 alpha Endosulphan 13C PCB 153

PCB IS

Deuterated PCB 38, C-13 labeled PCBs 15, 77, 126, 169, 28, 118, 105, 156, 52, 101, 

128, 180, 194, 208, 209

13C PCB 111( for Diortho PCB analysis), 

13C 101( for Mono Ortho & Non Ortho 

PCB analysis)

9 Pest. ES 1,3-DBB, Endrin Ketone

PCB ES PCB 30 and 204

10 Pest. ES Endrin

PCB ES DBOFB

11 Pest. IS Dibromooctafluorobiphenyl and octachloronaphthalene 1-bromo-2-nitrobenzene

PCB IS the same as the above the same as the above

12 Pest. ES

PCB NA

13 Pest. ES

PCB ES

14 Pest. ES

PCB ES

15 Pest. IS 1,3,5 TBB, 1,2,4,5 TTBB, d-HCH, 1,3 DBB, Endrin Ketone Octachloronapthalene

PCB IS Congener 30, 204 Octachloronapthalene

19 Pest. IS PCB 29 and PCB 112 

PCB IS PCB 29 and PCB 112 

17 Pest. IS Heptachlor

PCB IS PCB 199

18 Pest. IS ε-HCH, PCB 46, PCB 143

PCB IS PCB 46, PCB 143

19 Pest. ES

PCB IS

20 Pest. IS TCN (tetrachloronaphtalene)

PCB IS labeled PCBs 77, 126, 169

TCN, labelled PCBs 70*, 111*, 138*, 

170*

21 Pest. NA*

PCB NA

22 PBDE IS

(All 13C12-PBDEs) BDE-3, BDE-15, BDE-28, BDE-47, BDE-99, BDE-153, BDE-154, 

BDE-183, BDE-197, BDE-207, BDE-209 13C12-BDE-139

23 Pest. IS PCBs 103 and 198, DBOFBP TCMX

PCB IS PCBs 103 and 198, DBOFBP TCMX

A Fatty Acid IS C13:0 Fatty Acid C13:1 Fatty Acid

B Fatty Acid

C Fatty Acid C19:0

D Fatty Acid

E Fatty Acid

F Fatty Acid IS

*NA: Not stated
Summary of Methods Used

Laboratory Any Other Internal Standards? Added When?

IS Surrogate Standards Used for Quantitation 

Were Added:

If the IS/surrogates Added 

After Extraction/Cleanup, 

Were Results Corrected for 

Recovery?

1 Pest. Prior to extraction No

PCB

2 Pest. Prior to extraction No

PCB

3 Pest. Prior to extraction No

PCB

4 Pest. tetrachloro-m-xylene

Just before HPLC-SEC 

separation. Prior to extraction

PCB tetrachloro-m-xylene

5 Pest. Prior to extraction

PCB

6 Pest. 1,2,3-Trichlorobenzene (123-TCB) Before HPLC cleanup After extraction/cleanup prior to GC analysis No

PCB PCB 192

7 Pest.

PCB-30 / OCN prior to extraction, aldrin prior to 

analysis No

PCB

8 Pest. Prior to extraction Yes

PCB Yes

9 Pest. 1,3,5-TBB,1,2,4,5-TTBB,d-HCH, No

PCB

10 Pest. Prior to extraction

PCB

11 Pest. After extraction/cleanup prior to GC analysis No

PCB

12 Pest.

PCB

13 Pest.

PCB

14 Pest.

PCB

15 Pest. After extraction/cleanup prior to GC analysis Yes

PCB

16 Pest. Prior to extraction

PCB

17 Pest.

those added after extraction/cleanup and just prior to 

chromatographic analysis No

PCB

18 Pest. Prior to extraction

PCB

19 Pest.

PCB

20 Pest.

PCB

Prior to extraction for non-ortho PCBs; after 

extraction for the rest of PCBs

21 Pest.

PCB

22 PBDEs After extraction just prior to GPC Yes

23 Pest.

PCB PBB 77 Prior to extraction Yes - PBB 77

A Fatty Acid Fatty Acid C11:1 Prior to Extraction 88-109%

B Fatty Acid

C Fatty Acid

D Fatty Acid

E Fatty Acid

F Fatty Acid
Summary of Methods Used

Laboratory Recovery Range (% )

Were PCBs Separated 

From Pesticides Prior to 

GC?

Does PCB 132 coelute with PCB 

153 or with PCB 105 or is it 

separated from both?

Please Note Any Differences in 

Procedures Used for SRM 1945 

Analyses from Those Used for 

Homog. V 

1 Pest. Yes

132 Coelutes with 153, Resolved 

from 105

PCB

2 Pest. No Separated

PCB

3 Pest. No

PCB

4 Pest. 101-109 No Coelutes with 153

PCB 101-109 

5 Pest. 83-95 No

PCB 153 coelutes with other PCBs 

but not PCB 132

PCB 83-95 

6 Pest.

Ronnel: Homog. V (105 ± 44 ), 

SRM1945 (116 ± 4 ); 123-TCB: Homog. 

V (64 ± 12 ), SRM1945 (78 ± 24 ) No

132 Coelutes with 153, Resolved 

from 105

PCB

DOB: Homog. V (122 ± 60 ), 

SRM1945 (121 ± 4 ); PCB 198: Homog. 

V (169 ± 25 ), SRM1945 (140 ± 6 ); 

PCB 192: Homog. V (124 ± 13 ), 

SRM1945 (124 ± 10 )

7 Pest. Yes Separated

PCB PCB-30 (61-80 )

8 Pest. 60 to 120 No

132 Coelutes with 153, Resolved 

from 105

PCB 40 to 100 

9 Pest. Yes Separated

PCB

10 Pest. 86-140 Yes Coelutes with 105

PCB 81-87

11 Pest. 54-85 No Unknown

PCB 54-85

12 Pest. Yes

PCB

13 Pest. Yes Separated

PCB

14 Pest. No Quantitate only total Arochlor 1260

PCB

15 Pest. 32-80 Yes

PCB 52-80

16 Pest. 85 -111 No Separated

PCB 85 -111 

17 Pest. No Separated

PCB

18 Pest. 70-85 No Separated

PCB 75-90

19 Pest. Yes Separated

PCB 83-128

20 Pest. No Separated

PCB

21 Pest. No Unknown

PCB

22 PBDES 50-120 

23 Pest. 40-120 Only the Planar PCBs Coelutes

PCB 40-120
Additional analyte data and notes reported by individual laboratories

Appendix E
Additional Data and Notes

Lab Additional Data and Notes are Given Below

1 Homog V Homog V Homog V SRM 1945 SRM 1945 SRM 1945

Compound Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

(ng/g wet mass) (ng/g wet mass) (ng/g wet mass) (ng/g wet mass) (ng/g wet mass (ng/g wet mass)

PCB 8 <2 <2 <2 <2 <2 <2

PCB 29 <2 <2 <2 <2 <2 <2

PCB 45 <2 <2 <2 <2 <2 <2

PCB 63 <2 <2 <2 <2 <2 <2

PCB 74 11.6 10.9 11.3 13.5 13.9 12.8

PCB 70 3.2 2.9 3.1 11.1 11.9 10.3

PCB 56 <2 <2 <2 7.6 8.6 7.3

PCB 92 <2 <2 <2 17.4 17.6 17.9

PCB 110 <2 <2 <2 32.0 32.6 32.2

PCB 154 <2 <2 <2 0.8 0.7 1.1

PCB 82 <2 <2 <2 1.2 1.7 2.0

PCB 107 <2 <2 <2 4.9 5.3 5.3

PCB 146 19.7 19.5 19.9 31.9 32.5 32.7

PCB 158 47.3 47.9 48.5 30.2 30.9 30.4

PCB 163 2.4 2.1 2.8 7.1 6.4 7.3

PCB 174 <2 <2 <2 16.6 16.7 16.5

PCB 157 32.6 32.7 33.4 2.6 2.5 2.2

heptachlor <2 <2 <2 <2 <2 <2

2 Homog V Homog V Homog V SRM 1945 SRM 1945 SRM 1945

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

Compound (ng/g wet mass) (ng/g wet mass) (ng/g wet mass) (ng/g wet mass) (ng/g wet mass (ng/g wet mass)

PCB 138 & 158 190 189 182 115 117 116

PCB 180 & 193 691 679 665 140 129 139

4 Homog VI Homog VI Homog VI SRM 1945 SRM 1945 SRM 1945

Compound Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

(ng/g wet mass) (ng/g wet mass) (ng/g wet mass) (ng/g wet mass) (ng/g wet mass (ng/g wet mass)

17 <0.387 <0.397 <0.332 1.85 1.81 1.82

33 <1.55 <1.59 <1.33 <1.76 <1.68 <1.61

70 <1.55 <1.59 <1.33 10.1 10.1 10.1

74 13.0 12.9 12.8 16.9 16.6 16.8

82 <0.388 <0.398 <0.332 3.85 3.89 3.83

110 <1.56 <1.60 <1.34 34.9 34.5 34.9

171 2.96 3.06 3.08 12.2 12.1 12.2

177 <1.56 <1.60 <1.34 30.8 30.7 31.0

191 2.98 3.10 2.98 <1.78 <1.70 <1.63

199 5.51 5.59 5.41 82.8 82.0 82.7

205 9.75 9.94 9.49 1.83 1.8 1.83

208 <1.56 <1.60 <1.34 25.5 25.5 25.8

aldrin <1.55 <1.59 <1.33 2.02 1.98 2.32

endosulfan I <1.56 <1.60 <1.34 <1.78 <1.70 <1.63

endosulfan II 11.9 11.9 11.9 <1.81 <1.73 <1.66

endosulfan sulfate <1.57 <1.61 <1.34 <1.79 <1.71 <1.63

Please see supplemental worksheet for Fatty Acid analysis results.

5 I o,p'-DDT coleutes with PCBs and could not be quantitated in the Homogenate samples.

Only one level of calibration standard is used for quantitation, due to the extended linear response range of the detector (photodiode array).

REPORT ADDITIONAL ANALYT Homog VI Homog VI Homog VI SRM 1945 SRM 1945 SRM 1945

PCB 77 <1.1 <1.4 <1.2 <1.2 <1.3 <1.5

PCB 126 <0.92 <0.98 <0.86 <0.80 <0.90 <1.0

PCB 189: 10.2 9.76 11 < 0.61 < 0.66 < 0.74

∑PCBs 4900 5000 5000 1700 1500 1700

∑DDTs (I) 95 98 93 970 900 860

I o,p'-DDT coleutes with PCBs and could not be quantitated in the Homogenate samples.

Lipid Classes: Homog VI Homog VI Homog VI SRM 1945 SRM 1945 SRM 1945

Wax esters 0 0 0 1.72 2.14 1.66

Triglycerides 100 100 100 98.3 97.9 98.3

Free fatty acids 0 0 0 0 0 0

Cholesterol 0 0 0 0 0 0

Phospholipids 0 0 0 0 0 0

Lipid classes are reported as percent of total lipid fraction.

6 REPORT ADDITIONAL ANALYTICAL RESULTS INCLUDING OPTIONAL ANALYES IN THE SPACE BELOW

Notes:

1. Two other candidate standards were added to each sample: octachloronapthalene (OCN) and 3,3',4,4'-tetrabromobiphenyl (TBB) to assess their utility as

GC internal standards in the final chromatographic analysis. Using our gas chromatographic conditions, PCB 206 elutes very near the OCN peak. Unfortunately, 

too much OCN was added to each sample that made the identification and quantification of PCB 206 very difficult. The responses for TBB in each sample were 

reduced from those observed in our calibraiton solutions, which made it an unreliable standard for chromatographic analysis. We speculate that the reduced 

response of TBB may be related to matrix effects. In summary, we did not find either standard suitable for these particular analyses.

2. To prepare the calibration solutions for this intercomparison exercise, we used these solutions: SRM 2261 and SRM 2274 from NIST as the sources for 

the chlorinated pesticides. From Accustandard, Inc, New Haven, CT., we purchased the 20 PCB mixture C-CCSEC and the individual PCBs: 87, 132, and 201.

We also purchased the supplementary PCB solution SRM 2275 from NIST. 
Additional Data and Notes

3. We used hydrogen gas as the carrier gas at a linear velocity of 40 cm/min. For the analyses of PCBs and pesticides, 1 microliter of sample 

was injected into the GC-ECD.

4. Using our gas chromatographic conditions, PCB 153 and PCB 132 elute very closely to each other when a calibration solution is run. In the samples, 

the peak in this region of the chromatogram is very broad making identification and quantification of PCB 132 very difficult. PCB 105 is separated

from both PCB 153 and PCB 132.

Other: We found the following pairs of analytes coelute from our DB-5 column:

OtherA : 4,4’-DDD and cis-Nonachlor

OtherB : PCB 8 and alpha-HCH 

OtherC : PCB 87 and dieldrin

OtherD : PCBs 132 and 153 elute closely to each other. Conservatively, the concentration of PCB 153 given in the table should be treated as the sum of 

 PCB 153 and PCB 132.

OtherE : PCB 206 and the internal standard octachloronaphthalene

General Comment:

We use a nitrogen generator as the source of the make-up gas for the ECD. This nitrogen supply was interrupted during the analyses of the SRM samples. 

These analyses were resumed after the plumbing for the air supply for the generator was repaired. The ECD baseline for the SRM samples was elevated and

 very noisy compared to the baseline for the Homogenate VI samples.

Report Additional Analyte Results Below

Additional Homog VI Homog VI Homog VI SRM 1945 SRM 1945 SRM 1945

Pesticides and PCBs Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

(ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt)

Heptachlor 0.0 2.47 2.00 DL DL 10.9

Aldrin DL DL DL DL DL 5.91

Endrin DL DL DL 31.9 15.6 30.4

Endosulfan I 3.70 5.63 DL DL DL DL

Endosulfan II 31.0 43.8 13.0 DL 17.3 12.7

Endosulfan Sulfate 8.18 9.37 2.08 21.8 24.3 20.6

PCB 8 OtherB OtherB OtherB OtherB OtherB OtherB

PCB 77 OtherF OtherF OtherF OtherF OtherF OtherF

PCB 110 OtherF OtherF OtherF OtherF OtherF OtherF

PCB 126 DL DL DL DL DL 26.5

PCB 169 DL DL DL 14.4 58.7 19.4

Other: We found the following pairs of analytes coelute from our DB-5 column:

OtherB : PCB 8 and alpha-HCH 

OtherF : Since PCB 77and PCB 110 coelute from our column and the response of each congener vary from each other, the relative ratios

of two congeners can not be determined. We do not report the concentrations of either PCB 77 or PCB 110.

Other comments:

PCB 126 was detected in only one SRM 1945 sample.

7 PBDE CONGENER ANALYSES

Aproximately 1.5 grams extracted with hexane using a ball mill. % lipid determined gravimetrically using 10% of extract. Lipid removed from 

80% of extract by GPC, further cleaned on 1.2% deactivated Florisil. Analysed by GC-HRMS using 30 m DB-5MS (0.25 mm ID, 0.1 um film)

HRMS at RP = 10,000, EI mode. 

Homog VI Homog VI Homog VI SRM 1945 SRM 1945 SRM 1945

Congener Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

(ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt)

47 6.67 5.81 5.81 38.1 40.1 39.5

99 1.86 0.56 0.501 17.3 18.8 17.7

100 0.377 0.247 0.258 6.83 7.24 7.45

153 0.609 0.795 0.561 6.50 6.40 6.27

154 0.0209 0.0098 0.0032 11.3 11.0 11.0

8 * PCB 132 co elutes with PCB 153. PCBs 160/163/164/138 co elute. PCBs 187/182 co elute. PCBs 87/115 co elute

PCB 52 co elutes with PCB 73

Homog VI Homog VI Homog VI SRM 1945 SRM 1945 SRM 1945

Congener Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

(ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt)

PCB 4/10 DL DL DL DL DL DL

PCB 8/5 0.132 0.095 0.095 0.185 0.171 0.201

PCB 6 DL DL DL DL DL DL

PCB 7/9 DL DL DL DL DL DL

PCB 11 0.95 0.115 0.117 0.125 0.144 0.156

PCB 12 DL DL DL DL DL DL

PCB 13 DL DL DL 0.056 DL 0.03

PCB 14 DL DL DL DL DL DL

PCB 15 DL 0.071 0.064 0.15 0.162 0.18

PCB 16/32 ND 0.134 0.13 1.63 1.28 1.15

PCB 17 DL DL DL 1.04 0.641 0.841

PCB 19 DL DL DL 0.221 DL DL
Additional Data and Notes

PCB 33/20 0.109 0.094 0.080 0.846 0.709 0.686

PCB 21 DL DL DL DL DL DL

PCB 22 0.088 0.052 0.04 0.192 0.084 0.093

PCB 23/34 DL DL DL DL DL DL

PCB 27/24 DL DL DL 0.311 0.235 0.223

PCB 25 DL DL DL 0.097 0.054 0.071

PCB 26 DL DL DL 0.426 0.385 0.363

PCB 29 DL DL DL DL DL DL

PCB 30 DL DL DL DL DL DL

PCB 35 DL DL DL DL DL DL

PCB 36 DL DL DL DL DL DL

PCB 37 0.042 0.031 0.027 0.481 0.442 0.516

PCB 38 DL DL DL DL DL DL

PCB 39 0.013 0.006 0.007 0.009 0.011 0.013

PCB 40 DL DL DL 1.218 1.318 1.28

PCB 71/41/64 0.135 0.183 0.211 7.30 7.51 7.09

PCB 59/42 DL DL DL 4.74 4.892 5.01

PCB 43 DL DL DL DL DL DL

PCB 45 DL DL DL 1.02 0.802 0.949

PCB 46 DL DL DL 0.322 0.227 0.314

PCB 47/75/48 11.405 10.776 10.489 11.3 10.5 10.5

PCB 50 DL DL DL DL DL DL

PCB 51 DL DL DL 0.322 0.211 0.242

PCB 73/52 1.773 1.762 1.645 38.6 36.9 36.5

PCB 53 DL DL DL 0.628 0.573 0.611

PCB 54 DL DL DL DL DL DL

PCB 55 DL DL DL DL DL DL

PCB 56/60 0.351 0.352 0.334 5.83 5.77 5.77

PCB 57 DL DL DL DL DL DL

PCB 58 DL DL DL DL DL DL

PCB 61/74 13.72 15.701 15.47 17.8 17.4 17.7

PCB 62 DL DL DL DL DL DL

PCB 63 0.055 0.063 0.062 0.165 0.185 0.16

PCB 65 DL DL DL DL DL DL

PCB 67 DL DL DL DL 0.038 0.028

PCB 68 DL DL DL 0.216 0.13 0.082

PCB 69 DL DL DL DL DL DL

PCB 70/76 0.228 0.316 0.283 10.0 9.49 9.98

PCB 72 DL DL DL 0.478 0.486 0.444

PCB 77 0.02 0.011 0.016 0.4 0.351 0.353

PCB 78 DL DL DL DL DL DL

PCB 79 DL DL DL DL DL DL

PCB 80-see below other other other other other other

PCB 81 DL DL DL 0.035 0.047 0.042

PCB 82 DL DL DL 3.26 3.56 3.22

PCB 109/83 DL DL DL 2.44 2.30 2.19

PCB 92/84 0.581 0.509 0.500 16.4 16.1 15.6

PCB 85 17.8 19.2 17.3 13.3 14.9 13.1

PCB 97/86 DL DL DL 11.5 12.4 11.4

PCB 115/87 5.40 6.83 6.05 23.0 24.8 23.2

PCB 88 ND 0.102 ND 0.414 0.515 0.343

PCB 89 DL DL DL 7.409 7.331 6.387

PCB 101/90 4.33 4.23 4.32 88.4 91.1 85.9

PCB 94 DL DL DL 0.222 0.193 0.22

PCB 96 DL DL DL 0.250 0.247 0.278

PCB 98 DL DL DL DL DL DL

PCB 100 DL DL DL 0.679 0.716 0.696

PCB 103 DL DL DL 0.654 0.581 0.633

PCB 104 DL DL DL DL DL DL

PCB 108/107 0.713 0.764 0.69 3.85 3.31 3.31

PCB 110 0.484 0.848 0.692 24.1 32.0 27.1

PCB 111 ND 0.381 0.285 0.327 0.193 0.216

PCB 112 DL DL DL DL DL DL

PCB 113 DL DL DL DL DL DL

PCB 114 1.78 1.74 1.84 1.77 1.83 1.78

PCB 116/125/117 3.47 3.297 3.265 ND 0.203 0.149

PCB 119 0.618 1.29 1.43 3.87 4.60 4.19

PCB 120 NA 0.629 0.537 NA 0.538 0.711

PCB 121 DL DL DL DL DL DL

PCB 122 DL DL DL DL DL DL

PCB 123 ND ND ND 1.08 0.900 0.981

PCB 124 DL DL DL 0.384 0.338 0.303

PCB 126 0.011 0.021 0.023 0.160 0.145 0.143

PCB 127 DL DL DL DL DL DL

PCB 129 0.213 0.2 ND 1.37 1.27 2.91

PCB 130 4.21 4.17 4.12 21.9 19.9 20.8

PCB 142/131 NA 3.32 3.81 0.449 0.447 0.623

PCB 132/153 2906 2708 3071 305 286 297

PCB 133 DL DL DL DL DL DL

PCB 143/134 DL DL DL 3.10 3.03 3.03

PCB 135/144 0.405 0.397 0.399 18.1 16.9 17.5

PCB 136 0.212 0.17 0.112 10.9 10.6 10.7

PCB 137 27.1 25.2 26.1 8.12 8.02 8.22

PCB 160/163/164/138 350 331 357 219 213 209
Additional Data and Notes

PCB 139/140 DL 0.025 0.029 0.925 0.835 0.964

PCB 141 DL DL 0.358 18.3 17.7 17.8

PCB 145 DL DL DL DL DL DL

PCB 146/161 33.3 32.3 32.0 53.2 50.6 54.4

PCB 147 0.193 0.156 0.168 3.19 3.10 3.30

PCB 148 DL DL DL 0.538 0.351 0.471

PCB 150 DL DL DL 0.348 0.364 0.279

PCB 152 DL DL DL 0.09 0.05 0.081

PCB 154 0.148 0.13 0.143 4.09 3.56 3.91

PCB 155 DL DL DL 0.666 0.708 0.822

PCB 157 37.0 37.8 38.3 2.71 2.92 2.83

PCB 158 3.16 3.74 4.48 8.19 8.06 8.30

PCB 159 DL DL DL 0.141 0.073 0.103

PCB 162 0.369 0.249 0.179 0.866 0.704 0.765

PCB 165 NA 0.766 0.597 DL DL DL

PCB 166 5.18 5.78 5.76 0.659 0.781 0.579

PCB 167 1.389 1.719 1.541 4.53 5.12 5.15

PCB 168 DL DL DL 20.4 18.3 19.7

PCB 169 0.442 0.449 0.441 0.12 0.127 0.133

PCB 171 3.35 3.47 2.98 12.0 12.0 12.2

PCB 192/172 4.04 3.96 3.41 13.5 13.9 12.5

PCB 173 DL DL DL 0.579 0.537 0.334

PCB 174/181 0.608 0.619 0.515 30.5 29.5 28.0

PCB 175 ND 0.102 0.104 3.36 2.83 2.95

PCB 176 DL DL DL 8.12 7.50 8.03

PCB 177 0.767 0.707 0.63 30.9 30.2 30.2

PCB 178 4.13 3.65 3.16 20.0 20.3 19.3

PCB 179 0.282 0.235 0.225 18.0 17.6 17.9

PCB 180 882 862 862 150 162 147

PCB 187/182 9.63 10.00 8.84 159 154 156

PCB 184 DL DL DL 1.1 0.839 0.931

PCB 185 DL DL DL 4.06 3.53 3.87

PCB 186 DL DL DL DL DL DL

PCB 188 DL DL DL 1.07 0.819 0.930

PCB 189 4.25 4.74 2.99 0.919 0.964 1.155

PCB 191 1.87 2.81 2.43 0.881 0.865 0.891

PCB 193 14.4 12.2 13.3 7.81 7.38 6.71

PCB 203/196 12.8 11.6 10.7 104 101 98.5

PCB 197 0.142 0.142 0.163 7.01 5.83 6.20

PCB 198 0.113 0.118 0.089 3.04 2.67 2.85

PCB 199 DL DL DL 5.05 4.63 4.66

PCB 200 0.389 0.427 0.286 34.2 31.4 33.1

PCB 202 3.25 2.902 2.498 41.3 39.5 39.4

PCB 204 DL DL DL DL DL DL

PCB 205 0.689 3.393 1.633 0.441 0.322 0.438

PCB 207 0.270 0.212 0.23 14.6 14.0 15.1

PCB 208 0.420 0.331 0.373 22.2 21.8 22.4

Note PCB 80/66 co elute 

12 We don't do individual PCB congeners. We do screen for Arochlor series. For SRM 1945, we found Arochlor 1260 at 1260, 1640 and 1860 ng/g

respectively for the triplicates. 

13 Homog VI Homog VI Homog VI SRM 1945 SRM 1945 SRM 1945

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

Compound (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt)

PCB 77 <0.100 0.329 0.324 0.776 0.814 0.655

PCB 126 0.478 0.61 0.513 0.847 0.737 0.719

PCB 169 0.682 0.758 0.845 0.340 0.323 0.256

14 Homog VI Homog VI Homog VI SRM 1945 SRM 1945 SRM 1945

Compound Analysis A Analysis B Analysis C Analysis A Analysis B Analysis C

(ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt)

Aldrin 2.90 5.00 3.70 8.00 5.20 4.90

Endrin 10.6 9.50 12.8 40.5 45.5 35.4

Heptachlor 2.60 3.70 2.80 4.80 4.70 4.30

Lindane 2.80 3.20 3.20 3.60 3.00 3.50

Arochlor 1260 (Total) 1779 1583 1493 711 807 669

15

Please note that our lab has a coelution issue with the following congeners: 49-43, 87-81, 128-167, 187-182. The values reported above for these coeluting 

congeners may be overestimated.

16

*** Due computer problems where we not able to calculate this compounds.

Polybrominated Diphenyl Ethers Homog VI Homog VI Homog VI SRM 1945 SRM 1945 SRM 1945

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

Date(s) of measurements (m/d/yr) 10.30.03 10.30.03 10.30.03 10.30.03 10.30.03 10.30.03

PBDE 47 4.34 3.82 4.15 32.19 32.95 34.01

PBDE 99 0.36 0.34 0.40 16.63 16.34 16.12
Additional Data and Notes

PBDE 100 0.32 0.29 0.38 8.52 8.54 8.65

PBDE 153 0.68 0.77 0.66 7.70 7.81 7.10

PBDE 154 <0.15 <0.15 <0.15 11.37 10.77 10.35

Comments:

We are surprised by the patterns of PCB/pesticide in the two samples. We would like to know the lokations from where the samples are taken and/or if they are spiked. 

The pesticides and the PCBs are analyzed on the two different columns listed earlier. The results given are selected from the column with the best recovery and best seperation. 

18

PCB CONGENER ANALYSES Homog VI Homog VI Homog VI SRM 1945 SRM 1945 SRM 1945

Congener Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

(ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt)

47 17.4 18.3 18.8 14.8 14.1 13.8

74 12.5 12.8 13.4 13.9 15.1 14.7

110 0.78 0.63 0.64 32.6 30 31

157 44.3 43 44.2 7.75 8.11 7.76

167 2.21 2.3 2.39 6.77 6.79 6.96

174 0.57 0.69 0.79 23.7 22.9 23.6

177 0.83 0.97 0.94 26.6 25.5 26.1

190 82.7 79.6 83.3 7.97 7.97 7.96

196 10.5 10.5 10.9 76.5 75 76.2

199 5.43 4.98 4.9 75.1 76.5 76.7

Octachlorostyrene 4.01 3.95 5.09 12.7 9.45 8.7

PBDEs-PBBs

Analytical method used (e.g., GC-MS (include ionization type), GC-ECD):

Analyt. Instr. Column Phase Col. Length, m Col. i.d., mm Col. film thickness, μm

PBDEs-PBBs GC/MS (ECNI) HT-8 25 0.22 0.25

Method of quantitation (IS = internal standard, ES = external standard):

PBDEs-PBBs IS

IF internal standard method was used, please complete the following section:

Identity of internal standards/surrogates used that were added PRIOR to extraction of sample:

PBDEs-PBBs BB103 and BB 155

 Added after extraction/cleanup and JUST PRIOR to chromatographic analysis:

PBDEs-PBBs -

 IS/surrogate standards used for quantitation calculations were: 

Yes those added prior to extraction

No those added after extraction/cleanup and just prior to chromatographic analysis

Calibration Curve

Analytes outside of calibration 

Points Conc. Range curve calibration range

PBDEs-PBBs 8 __________________ none

RESULTS:

PERCENT LIPID (List each result if determined more than once. Enter results as a number, for example 90.0. DO NOT change format of cell to percent.)

PBDE-PBB ANALYSES Homog VI Homog VI Homog VI SRM 1945 SRM 1945 SRM 1945

PBDE-PBB Analysis A Analysis B Analysis C Analysis A Analysis B Analysis C

(ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt)

BDE 28 <0.05 <0.05 <0.05 1.23 1.25 1.25

BDE 47 4.51 4.49 4.44 35 35.6 36.1

BDE 99 0.25 0.44 0.55 16.8 16.8 17.2

BDE 100 0.43 0.35 0.37 8.56 8.72 8.9

BDE 153 0.98 1.03 1.18 7.5 7.26 7.5

BDE 154 0.23 0.19 0.12 10.8 10.4 10.9

BDE 183 0.24 0.22 0.36 1.56 1.53 1.55

BB 153 3.13 2.85 2.96 3.24 3.09 3.22

20

Analytical method used (e.g., GCMS (include ionization type), GCECD):

Analyt. Instr. Column Phase Col. Length, m Col. i.d., mm film thickness, μm

non-ortho PCBs GC-MS(ITD) BPX-5 60 0.25 0.25

mono-ortho PCBs + 149+132 MDGC-ECD DB-5 / HT-8 30 / 30 0,25 / 0,25 0,25 / 0,25

"rest" PCBs GC-μECD DB-5 60 0.25 0.25

Calibration Curve

Analytes outside of calibration 

Points Conc. Range curve calibration range

non-ortho PCBs 7 0,1-800 pg/μl __________________________________

mono-ortho PCBs + 149+132 7 1,0-250 pg/μl __________________________________

"rest" PCBs 7 0,5-100 pg/μl __________________________________
Additional Data and Notes

Homog VI Homog VI Homog VI SRM 1945 SRM 1945 SRM 1945

Congener Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

(ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt)

81 <0,000401 __________ __________ 0.0468 __________ __________ 

77 0.0171 __________ __________ 0.279 __________ __________ 

126 0.0323 __________ __________ 0.161 __________ __________ 

169 0.314 __________ __________ 0.0808 __________ __________ 

114 1.75 __________ __________ ND __________ __________ 

157 34.8 __________ __________ 0.465 __________ __________ 

167 ND __________ __________ 0.471 __________ __________ 

189 6.77 __________ __________ 1.39 __________ __________ 
Additional Data and Notes

21 Sample Preparation

2.04 grams of Marine Mammal Blubber Control Material VI and 2.07 grams of Standard Reference Material 1945 “Organics in Whale Blubber”

 were respectively placed in separate 250 mL Erlenmeyer flasks. 25 grams of anyhydrous sodium sulfate were added to each flask.

50 mL of 5% ethanol in ethyl acetate was added to each flask, and then the samples were homogenized for 2 minutes. Following

centrifugation, the supernatant from each sample was collected. The samples were passed through separate silica gel solid phase

extraction columns, and an additional 6 mL of 5% ethanol in ethyl acetate was poured through each column. 

The collected samples were then evaporated to dryness under a stream of nitrogen, and were reconstituted with 1 mL of toluene.

Chromatographic Analysis

Samples were analyzed on a Varian 3800 GC with Saturn 2000 MS/MS detector. Splitless injection of 1 microliter sample was performed. 

Low – High Mass Range: 40 – 450 m/z

Injector Temp: 325

Hold Time: 30.95 min

Pressure: 25.0 psi

Blubber Sample: Marine Mammal Blubber Control Material VI

No organochlorine pesticides detected.

From Table 2, optional compounds, the following fatty acids were detected:

(Z,Z,Z,Z,Z,Z)-4,7,10,13,16,19-Docosahexaenoic Acid (Gadoleic Acid)

Tetradecanoic acid (Myristic Acid)

(Z)-9-Octadecanoic acid (Oleic Acid)

Pentadecanoic acid (Lauric Acid)

Eicosanoic acid (Arachidic Acid)

(Z)-9-Hexadecanoic acid (Palmitoleic Acid)

Hexadecanoic acid (Palmitic Acid)

(E)-9-Octadecenoic acid (Elaidic Acid)

(Z,Z,Z,Z)-5,8,11,14-Eicosatrienoic acid (Arachidonic Acid)

Miscellaneous compounds detected:

Niacinamide

Vitamin E

Cholesterol

Squalene

Blubber Sample: Standard Reference Material 1945 “Organics in Whale Blubber”

From Table 2, optional compounds, the following fatty acids were detected:

Dodecanoic acid (Lauric Acid)

Tetradecanoic acid (Myristic Acid)

Pentadecanoic acid (Lauric Acid)

(Z)-9-Hexadecanoic acid (Palmitoleic Acid)

(Z)-9-Octadecanoic acid (Oleic Acid)

(E)-9-Octadecenoic acid (Elaidic Acid)

Heptadecanoic acid (Margaric Acid)

(Z)-13-Docosenoic acid (Erucic Acid)

(Z,Z,Z,Z)-5,8,11,14-Eicosatetraenoic acid (Arachidonic Acid)

(Z)-11-Eicosenoic acid (Nervonic Acid)

(Z,Z,Z,Z,Z)-5,8,11,14,17-Eicosapentaenoic acid (Gadoleic Acid)

(Z,Z,Z,Z,Z,Z)-4,7,10,13,16,19-Docosahexaenoic Acid (Gadoleic Acid)

Detected the following furans:

2,5-dimethyl-2-(tetrahydrofuryl)tetrahydrofuran

2-propylfuran

Miscellaneous compounds detected:

Vanillin

4-chloro-3-n-hexyltetrahydropyrane

Retinol

Vitamin A aldehyde

Cholesterol

Squalene

Vitamin E

Standard Analysis

Pesticide Standard Mix A was purchased from Restek. A chromatogram yielding the following pesticides was generated and compared to the blubber chromatograms:

2,4,5,6-Tetrachloro-m-xylene

alpha-BHC

gamma-BHC (lindane)

Heptachlor

Endosulfan I

Dieldrin

Endrin

4,4’-DDD

4,4’-DDT

Methoxychlor

Comparative analysis confirmed the absence of pesticides in the blubber samples.
Additional Data and Notes

22 Homog VI Homog VI Homog VI SRM 1945 SRM 1945 SRM 1945

PBDEs Analysis A Analysis B Analysis C Analysis A Analysis B Analysis C

(ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt)

BDE15 DL DL DL 0.0248 0.0232 0.0277

BDE28 DL DL DL 1.02 0.974 1.06

BDE47 4.78 4.09 4.27 33.0 32.7 34.9

BDE99 0.396 0.351 0.376 15.7 15.5 16.7

BDE100 0.310 0.223 0.257 8.77 8.65 9.33

BDE153 0.876 0.684 0.763 7.87 7.91 8.33

BDE154 0.0468 DL 0.0406 11.8 12.2 12.3

BDE183 DL DL DL 1.64 1.61 1.72

BDE196 DL DL DL 0.0917 0.0934 0.0966

BDE197 DL DL DL 0.394 0.410 0.462

BDE206 DL DL DL DL DL DL

BDE207 DL DL DL DL DL DL

BDE209 1.02 0.709 0.772 DL DL DL

Total PBDEs 7.43 6.06 6.47 80.2 80.0 85.0

A C20:1n15 0.277 0.272 0.153 0.139 0.144

C20:3n6 1.46 1.44 0.468 0.459 0.517

C22:0 (behenic acid) 0.117 0.101 0.268 0.288 0.283

C22:4n6 3.05 3.06 0.82 0.932 <0.1

C22:3n3 <0.2 <0.2 <0.2 <0.2 <0.2

C24:0 0.145 0.141 0.166 0.19 0.167

Homog VI Homog VI SRM 1945 SRM 1945 SRM 1945

Analysis A Analysis B Analysis A Analysis B Analysis C

(g/g wet wt x 100) (g/g wet wt x 100) (g/g wet wt x 100) (g/g wet wt x 100) /g wet wt x 100)

C14:1n9 < 0.00927 < 0.00925 0.102 0.102 0.103

4,8,12-trimethyl-C13:0 0.0124 0.0118 0.0291 0.0283 0.0301

C14:1n7 0.0342 0.0334 0.130 0.130 0.131

11-methyl-C14:0 < 0.00502 < 0.00501 0.00533 < 0.00543 < 0.00595

anteiso-C15:0 0.0391 0.0379 0.0648 0.0656 0.0676

anteiso-C16:0 0.00629 0.00692 < 0.00449 < 0.00477 < 0.00522

2,6,10,14-tetramethyl-C15:0 0.0254 0.0248 0.0715 0.0724 0.0712

C16:1n11 0.170 0.168 0.184 0.179 0.182

C16:1n9 0.324 0.321 2.01 2.03 2.08

C16:1n5 0.135 0.135 0.205 0.207 0.214

7-methyl-C16:1n? 0.0209 0.0203 0.0671 0.0666 0.0669

C16:2n6 0.0398 0.0390 0.0275 0.0233 0.0254

anteiso-C17:0 0.125 0.124 0.0709 0.0719 0.0748

7,8-dimethyl-C16:1n? < 0.00804 < 0.00802 < 0.00818 < 0.00870 < 0.00953

C16:2n4 0.278 0.276 0.172 0.171 0.174

C16:3n6 0.0314 0.0355 < 0.0189 < 0.02 < 0.022

C16:3n4 0.0545 0.0593 0.0334 0.0348 0.0314

C17:1n? 0.302 0.298 0.392 0.390 0.403

C16:4n3 0.0497 0.0484 0.155 0.156 0.150

anteiso-C18:0 0.0170 0.0168 0.00442 0.00477 0.00494

C16:4n1 0.0719 0.0699 < 0.0298 < 0.0316 < 0.0346

C18:1n13 0.111 0.116 0.0548 0.0491 0.0513

C18:1n11 1.03 1.02 2.54 2.56 2.66

C18:1n5 0.324 0.323 0.256 0.261 0.267

C18:2n7 0.121 0.120 0.0277 0.0304 0.0338

C18:2n4 0.176 0.170 0.0559 0.0565 0.0585

C18:3n4 0.240 0.242 0.0307 0.0294 0.0269

C18:3n1 < 0.0185 < 0.0185 < 0.0189 < 0.0200 < 0.0220

C18:4n1 0.0950 0.0953 < 0.0298 < 0.0316 < 0.0346

C20:1n5 0.0982 0.0968 0.0491 0.0485 0.0506

C20:1n11 1.19 1.33 3.15 3.14 3.26

C20:2n11 0.178 0.200 0.156 0.180 0.175

C20:2n9 0.0565 0.0576 < 0.00943 < 0.0100 < 0.0110

C20:4n3 0.299 0.296 0.200 0.194 0.196

C22:1n11 0.606 0.604 3.92 3.93 3.98

C22:1n7 0.118 0.119 0.103 0.105 0.108

C22:1n5 < 0.0185 < 0.0185 < 0.0189 < 0.0200 < 0.0220

C21:5n3 0.371 0.367 0.106 < 0.0865 0.104

C22:4n3 0.103 0.0982 < 0.0814 < 0.0865 < 0.0948

B

Fatty Acid Analyses

Co mpound Homog VI Homog VI Homog VI SRM 1945 SRM 1945 SRM 1945

Analysis A Analysis B Analysis C Analysis A Analysis B Analysis C

mg/g wet mg/g wet mg/g wet mg/g wet mg/g wet mg/g wet

C13:0 0.069 0.065 0.065 0.229 0.174 0.171

Iso-C15:0 0.919 0.814 0.794 2.052 1.559 1.540

C14:1n5 2.830 2.611 2.495 4.074 3.056 3.028

Ante-C15:0 0.941 0.834 0.791 1.446 1.081 1.099

C15:1n5 0.000 0.000 0.000 0.000 0.000 0.000

C17:1n7 0.000 0.000 0.000 0.000 0.000 0.000

C18:1n11 9.686 6.645 6.300 20.898 17.330 21.514

These fatty acids are tentative IDs and are quantified using response factors from an equivalent isomer from the list above, reported as a mass fraction percent on a wet weight 

basis (as FAMEs).
Additional Data and Notes

C18:2n6t 0.000 0.000 0.000 0.300 0.242 0.230

C20:3n6 0.969 0.940 0.949 0.335 0.207 0.263

C22:0 0.000 0.000 0.000 0.000 0.000 0.000

C24:0 0.000 0.000 0.000 0.059 0.040 0.069

Sats 0.014 0.014 0.013 0.025 0.017 0.012

Mono 0.068 0.067 0.067 0.060 0.069 0.076

PUFA 0.018 0.019 0.020 0.051 0.014 0.012

w3 0.017 0.018 0.018 0.004 0.012 0.010

w6 0.002 0.002 0.002 0.011 0.002 0.002

w3/w6 0.010 0.011 0.011 0.000 0.007 0.007

trans 0.001 0.001 0.001 0.009 0.001 0.001

C Compound values expressed mg/g wet tissue

MM VI AA4 MM VI AA5 MM VI AA6 1945 AA1 1945 AA2 1945 AA3

C 13:0 <0.17 <0.17 <0.17 0.36 0.36 0.40

C14:1 4.92 4.91 5.04 6.11 6.27 6.37

C20:3 0.36 0.45 0.43 1.96 2.39 2.42

C 22:4 3.73 3.59 3.75 0.94 0.88 0.98

sum 870 829 916 713 730 735

D

values expressed mg/g wet tissue

Compound Homogenate VI SRM 1945

mg/g mg/g

C6:0 0.000 0.000

C8:0 0.000 0.000

C10:0 0.039 0.052

C11:0 0.065 0.186

C13:0 0.186 0.055

C14:1n5 0.645 0.773

C15:1 0.000 0.004

C17:1 0.586 0.441

C18:1 ND ND

C18:2n6t 0.041 0.070

C20:0 0.183 0.185

C21:0 0.000 0.000

C22:0 0.065 0.075

C20:3n6 2.614 6.587

C23:0 0.000 0.000

C24:0 0.012 0.130

C26:0 ND ND

Σω3 9.50 9.61

Σω6 4.41 8.35

ΣSAFA 17.8 19.4

ΣMUFA 37.2 33.1

ΣPUFA 15.7 18.3

E

Compound Expressed as percent of total Fas

iso + antiso C5:0 Homog VI Homog VI Homog VI 1945-1 1945-2 1945-3

4,8,12-trimethyltridecanoic acid 

(TMTD) <0.01 <0.01 <0.01 1.69 1.66 1.48

(Z)-9-tetradecenoic acid (14:1n-5) 0.057 0.055 0.056 0.119 0.116 0.114

iso 15:0 0.501 0.496 0.5 0.663 0.648 0.643

antiso 15:0 0.174 0.173 0.173 0.329 0.325 0.315

(Z)-7-hexadecenoic acid (16:1n-9) 0.0682 0.067 0.0695 0.126 0.121 0.123

(Z,Z)-6,9-Hexadecadieonic acid (16:2n7) 0.445 0.433 0.435 2.83 2.75 2.65

(Z,Z)-9,12-Hexadecadieonic acid (16:2n

4) 0.0571 0.0567 0.0547 0.0565 0.0563 0.0564

Iso17 0.14 0.137 0.138 0.207 0.206 0.212

antiso17:0 0.279 0.279 0.28 0.171 0.169 0.172

7-methyl-7-hexadecenoic acid (7M7H) 0.473 0.47 0.47 0.098 0.097 0.099

phytanate 0.171 0.168 0.168 0.565 0.546 0.574

(Z)-9-heptadecenoic acid (17:1n-8) 0.112 0.107 0.113 0.123 0.108 0.088

(Z)-7-octadecenoic acid (18:1n-11) 0.385 0.404 0.396 0.519 0.502 0.514

(Z)-13-octadecenoic acid (18:1n-5) 1.59 1.57 1.24 3.17 2.92 4.06

(Z,Z)-11,14-Octadecadienoic 

acid (18:2n-4) 0.456 0.451 0.454 0.352 0.353 0.355

(Z,Z,Z)-8,11,14-Eicosatrienoic acid 

(20:3n-6) 0.284 0.293 0.281 0.0926 0.0896 0.0896

F Compound Expressed as percent of total Fas

Homog VI Homog VI Homog VI 1945-1 1945-2 1945-3

13:0 0.17% 0.15% 0.14%

14:1n10 0.14% 0.17% 0.16%

14:1n7 0.55% 0.50% 0.58% 0.21% 0.23% 0.22%

14:1n5 0.78% 0.71% 0.77%

6-me 15:0 0.00% 0.00% 0.00% 0.18% 0.18% 0.20%
Additional Data and Notes

iso 15 0.19% 0.19% 0.18% 0.38% 0.34% 0.40%

anteiso 15 0.06% 0.10% 0.08% 0.13% 0.14% 0.18%

16:1n9 0.44% 0.33% 0.55% 3.29% 3.12% 3.29%

Iso 16 0.20% 0.15% 0.24%

16:2n4 0.48% 0.53% 0.55%

16:1n5 0.31% 0.25% 0.32%

17:1n10 0.21% 0.16% 0.21% 0.80% 0.65% 0.76%

iso 17:0 0.30% 0.26% 0.34% 0.35% 0.24% 0.27%

17:1n8 0.43% 0.36% 0.51% 0.69% 0.58% 0.66%

Anteiso 17 0.25% 0.27% 0.28%

3,7,11, 15-tetra methyl 20:0 0.23% 0.26% 0.23% 0.54% 0.42% 0.46%

18:1n11 2.92% 2.43% 2.58% 5.67% 5.49% 5.38%

18:1n5 0.51% 0.36% 0.63% 0.53% 0.48% 0.28%

20:4n6 0.40% 0.36% 0.40%

20:1n13 1.27% 1.39% 1.53%

20:1n5 3.92% 4.19% 3.98% 0.77% 0.71% 0.70%