## Importation of **Fresh Fruit of Pomegranate**  **, Punica granatum**  **L. ,**  **from India into the Continental United States**  **A Qualitative, Pathway -**  **initiated Pest**  **Risk Assessment** 

**August 17 , 2010**  **Revision 0 4** 

**Agency Contact:** Plant Epidemiology and Risk Analysis Laboratory Center for Plant Health Science and Technology United States Department of Agriculture Animal Plant Health Inspection Services Plant Protection and Quarantine 1730 Varsity Drive, Suite 300 Raleigh, NC 27606Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 ii 

### Executive Summary India has requested approval to export fresh pomegranate fruit , Punica granatum L. **,** into the continental United States , including Alaska. Because this commodity has not been imported from India before, the United States Department of Agriculture (USDA) , Animal and Plant Health Inspection Service (APHIS) conducted a pathway
- initiated risk assessment to determine the unmitigated risks associated with importing fresh pomegranate fruit from India. APHI S anal ys ts first prepared a list of pests in India that are known to be associated with Punica granatum. We compiled the pest list from standard sources , including documents submitted by India, records of intercepted pests, and scientific literature. We determined which quarantine pests were likel y to follow the pomegranate pathway , and qualitatively analy zed them to determine the unmitigated risk each poses to the United States. We identified the following pests with **H igh**  pest risk potential: Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) Bactrocera zonata (Saunders) (Diptera: Tephritidae) Conogethes punctiferalis (Guenée) (Lepidoptera: Crambidae) Cryptoblabes gnidiella Millière (Lepidoptera: Pyralidae) Deudorix epijarbas (Moore) (Lepidoptera: Lycaenidae) Deudorix isocrates Fabricius (Lepidoptera: Lycaenidae) Drosicha dalbergiae (Green) (Hemiptera: Margarodidae) Euzophera bigella Zeller (Lepidoptera: P yr alidae) Icerya seychellarum Westwood (Hemiptera: Margarodidae) Nipaecoccus viridis (Newstead) (Hemiptera: Pseudococcidae) Planococcus lilacinus (Cockerell) (Hemiptera: Pseudococcidae) Pseudococcus cryptus Hempel (Hemiptera: Pseudococcidae) We identified the following pests w ith **M edium**  risk potential: Bactrocera carambolae (Drew & Hancock) (Diptera: Tephritidae) Ceroplastes rubens (Maskell) (Hemiptera: Coccidae) Ceroplastes rusci (Linnaeus) (Hemiptera : Coccidae) Dichocrocis megillalis Walker (Lepidoptera: Pyralidae) Dysmicoccus neobrevipes Beardsley (Hemiptera: Pseudococcidae) Icerya aegyptiaca (Douglas) (Hemiptera: Margarodidae) Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae) Planococcus angkorensis (Takahashi) (Hemiptera: Pseudococcidae) Rhipiphorothrips cruentatus Hood (Thy sanoptera: Thripidae) Scirtothrips oligochaetus Kary ny (Thys anoptera: Thripidae) Tenuipalpus granati Sayed (Acari: Tenuipalpidae) Tenuipalpus punicae Pritchard & Baker (Acari: Tenuipalpidae) Xanthomonas axonopodis pv. Punicae (Hingorani & Singh) (Xanthomonads)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 iii 

### Table of Contents **Executive Summary ................................**  **................................ ................................**  **...................... ii**  **1. Introduction ................................**  **................................ ................................**  **................................ 1**  **2. Risk Assessment ................................**  **................................ ................................**  **......................... 2**  2.1. Initiating Event: Proposed Action ................................ ................................ ...................... 2 2.2. Assessment of Weed Potential ................................ ................................ ........................... 2 2.3. Previous Risk Assessment, Current Status, Pest Interceptions and Decision History Potential ................................ ................................ ................................ ................................ .... 3 2.4. Pest Categorization: Identification of Quarantine Pests and Quarantine Pests Li kel y to Follow the Pathway ................................ ................................ ................................ ................... 4 2.5. Quarantine Pests Likely to Follow the Pathway ................................ .............................. 29 2.6. Consequences of Introduction ................................ ................................ .......................... 31 2.7. Likelihood of Introduction ................................ ................................ ............................... 64 **3. Conclusion ................................**  **................................ ................................**  **................................ 69**  **4. References ................................**  **................................ ................................**  **................................ .**  **70 5. Authors and Reviewers**  **................................ ................................**  **................................ ............**  **88 Appendix: Risk Mitigation Options for Importation of Fresh Pomegranates (**  **Punica granatum**  **) Fruit from India into the Continental United States including Alaska ..........**  **89** 1. Introduction ................................ ................................ ................................ ......................... 89 2. Risk Mitigati on Options ................................ ................................ ................................ ...... 90 3. Conclusions ................................ ................................ ................................ ......................... 95 4. Literature Cited ................................ ................................ ................................ ................... 97Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 1 

### 1 **. Introduction**  This pest risk assessment was written and reviewed by staff at the Pest Epidemiology and Risk Analy sis Laboratory

(PERAL) of the United States Department of Agriculture (USDA). We examined the unmitigated risks associated with the importation of fresh pomegranate fruit, Punica granatum L. , int o the continental United States from India. This document is a qualitative risk assessment , and we expressed risk in terms of “high, ” “medium, ” and “ low ,” rather than in numerical terms (e.g., probabilities or frequencies ) . The details of the methodology and rating criteria can be found in Pathway
-Initiated Pest Risk Assessments: Guidelines for Qualitative Assessments, Version 5.02 (USDA, 2000) International plant protection organizations, such as the North American Plant P rotection Organization (NAPPO) and the International Plant Protection Convention (IPPC) provide standards for conducting pest risk anal ys es. The methods used to initiate, conduct, and report this assessment, as well as the use of biological and phy tosanitary terms are based on these standards ( IP PC, 2007 : I SPM #s 2, 4, and 11 ; NAPPO, 2004 ). The IPPC standards describe three stages of pest risk anal ys is: Stage 1, Initiation; Stage 2, Risk Assessment; and Stage 3, Risk Management. This document satisfies the requirements of Stages 1 and 2, and provides mitigation options for Stage 3. Pom egranate is a small tree or shrub that is 20
- 30 feet high ( Morton, 1987 ). The fruit are approximately

6 - 12 cm in diameter. It has a thick, leathery rind that protect 5
- 6 compartments filled with tightly

packed sacks filled with a red or white fleshy fruit , called arils, surrounding a 2 - 3 cm oblong, hard seed ( Morton, 1987 ). S eeds are ty picall y about 52 percent of the total weight of a pomegranate ( Morton, 1987 ). Insect pests are frequentl y found under the sepals surrounding the area where the stem joins the fruit . Diseases are usuall y not visible unless they directly affect the rind, because the thick rind prevents observ ation of internal damage . Pomegranate fruit are harvested by hand and the peduncle is removed to avoid fruit damage (USEP, 2007 ). Pomegranates are produced in India November through June. India expects to initially ship approximately

1500 tons of pomegranates per ye ar to the United States. Shipments will be made primarily by sea in 4 kg corrugated open tray boxes. Temperature will be maintained at 5 to 7 degrees Celcius (India market access request). Other than minimal post
- harvest cleaning and culling, we assumed for the purposes of this risk assessment, that po megranate fruit from India would not undergo any s pecial post
- harvest treatment or cleaning. Strategies for pest management of pomegranates in India include cultural controls such as field sanitation and the removal of weeds that may serve as an alternat e host for many pests; mechanical controls such as the hand removal of caterpillars and other pests, traps for monitoring of Lepidopteran pests, and phy sical barriers; biological control agents including predators and pathogens; and chemical controls (Indi a market access request).Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 2 

### 2 **. Risk**  **Assessment 2.1**  **. Initiating Event:**  **Proposed Action** India requested export of fresh pomegranates to the continental United States. Entry of this commodity

into the United States presents a potential pathway for the introduction of plant pests. Title 7, Part 319, Section 56 of the United States Code of Federal Regulations (7 CFR §319.56) grants regulatory

authority to the United States Department of Agriculture for the importation of fruits and vegetables from foreign sources. Importation of fruits and vegetables is regulated because plant pests have the potential to follow the pathway into the United States and become established pests that impact U.S. agricultural and natural res ources. **2.2 .**  **Assessment of Weed Potential** We assessed the potential of pomegranates to become weeds after they enter the United States ( Table 1 ) . A formal weed risk assessment was not conducted because the baseline assessments did not indicate that the commodity had a significant weed potential above existing levels. **Table 1.** The Weed Potential of Pomegranate **Scientific name:** Punica granatum L. (Ly thraceae) (Graham et al
. , 2005) **Common Names:** Pomegranate, grenadier, Granatapfelbaum, Granatapfelstrauch, zakuro, romanzeiro, romã, romã - de
- flor
- dobrada, romeira, romeira
- da
- granada, granado, mangrano (ARS, 2007). **Synonyms:** None (ARS, 2007). **Phase 1:** Distribution in the United States: P
. granatum is native to western Asia

(Afghanistan, Iran, Iraq, Turkey , Armenia, Azerbaijan, Georgia, Russian Federation, Tajikistan, Turkmenistan, and Pakistan) (ARS, 2007). It is under cultivation in the United States, at least in California and Arizona (CRFG, 2007), and has escaped or naturalized in AL , CA, FL, LA, NC, UT (NRCS , 2007). This species is hardy to USDA Plant Hardiness Zones 7
- 11 (Page and Olds, 2001). **Phase 2:** Invasive / Weed Status: Listing as weed No 7 CFR § 360, 2007; Gunn and Ritchie, 1988; Holm et al
. , 1997; Holm et al
. , 1979; Holm et al
. , 1977; Reed, 1977; Skinner et al
. , 2005; Weber, 2003; WSSA, 2006 Yes Alien plant invaders of natural areas (Swearingen, 2005). Yes A Global Compendium of W eeds (Randall, 2007). Yes Other: (Liogier and Martorell, 2000; LR, 2005; NRCS , 2007) . **Phase 3:** Summary

and Conclusions: Punica granatum is a warm temperate species that has escaped from cultivation in the United States, Australia, New Zealand and Puerto Rico (Liogier and Martorell, 2000 ; Randall, 2007; Swearingen, 2005); however, the literature does not indicate that it is invasive (i.e., with self
sustaining populations that are expanding, spreading, and causing environmental harm). Because P. granatum is widely distributed in the conti nental United States and not under official control, it does not meet the IPPC definition of a quarantine pest (IPPC, 2007: I SPM #5). Thus, this pest risk assessment continues.Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 3 **2.3 .**  **Previous Risk Assessment, Current Status, Pest Interceptions and Decision History**  **Potential 2.3.1**  **. Decision History and Current Status**  Several countries have requested permission to export pomegranates to the United States (USDA, 2007a). Most of these were approved, but Iran, Ital y, and Greece were d enied for various reasons ( Table 2 ) . **Table 2.** P rior decisions by the United S tates about requests to import Punica granatum . **Year Country**  **Decision Reasons / Conditions**  1924 Mexico Approved From fruit fl y- free areas of Mexico only ; with inspection and a phyt osanitary certificate. 1925 Ital y Denied Several major pests may be associated with pomegranate 1925 Greece Approved Inspection; T107(a) treatment mandatory for fruit fly . 1969 Argentina Approved Entry into or through California 1971 Haiti Approved North Atlantic & Pacific ports; cold treatment 1987 Greece Approved Inspection and treatment 1988 Turkey Approved Inspection; T107(a) treatment mandatory for fruit fly . 1988 Iran Denied No acceptable treatment for two species of fruit fly . 1988 Colombia Approved Inspection and treatment (T107
- c) for Anastrepha fraterculus & A. ludens . 1990 Mexico Approved Inspection and treatment if necessary 1997 Is rael Approved Treat for Medfl y **2.3.2 .**  **Previous Risk Assessments** The Animal and Plant Health Inspection Service (APHI S) conducted a pest risk assessment on P
. granatum once before: “ Importation of Pomegranate Fruits, Punica granatum from Israel into the United States ” ( PPQ, 1997 ). This risk assessment found that Ceratitis capitata , Deudorix isocrates , and Planococcus lilacinus posed a high risk to the United States, while Sphaceloma punic ae and Cryptoblabes gnidiella posed a medium risk . **2.3.3 .**  **Current Import Status** According to Plant Protection and Quarantine’s Fresh Fruit and Vegetable Import Manu al ( PPQ, 2007 ), pomegranates are currentl y enterable from Argentina, Colombia, Greece, Haiti, and Israel after irradiation (T105
- d) or cold treatment (T107
- a - 1, T107 - a, or T107 - c) . Pomegranates are enterable from Mexico from fruit fly free areas. **2.3.4 .**  **United States Pest Interceptions**  APHI S - PPQ records all interceptions of pests and pathogens on commodities entering the United States ( PestID , 2007) . The database includes interceptions since 1985 on permit cargo, general cargo, mail , passenger baggage , ships ’ quarters , stores and holds, and other locations . WePest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 4 searched this database for pests and pathogens intercepted on pomegranate fruit from the world . O ver 99 percent of the interceptions were on baggage and cargo, with the vast majority of interceptions on passenger baggage (6927 , vs. 37 in cargo ). Of the 345 taxa that have been intercepted, 26 have been intercepted 20 times or more ( Table 3 ). **Table 3.** Pest taxa most frequentl y intercepted on Punica granatum at U.S. Ports of Entry , from 1985 to 2007. **Pest Baggage**  **Cargo** Sphaceloma punicae Bitanc. & Jenkins (Coelomycetes) 4175 4 Pseudococcidae, species of 452 5 Cryptoblabes gnidiella (Milliere) (Py ralidae) 311 2 Anastrepha sp. (Tephritidae) 246 1 Ectomyelois ceratoniae (Zeller) (P yr alidae) 114 2 Cryptoblabes sp. (Py

ralidae) 89 1 Ceratitis capitata (Wiedemann) (Tephritidae) 84 Planococcus citri (Risso) (Pseudococcidae) 65 Oxycarenus hyalinipennis (Costa) (Ox yc arenidae) 60 Planococcus sp. (Pseudococcidae) 59 1 Pseudocercospora punicae (Henn.) Deighton (Hyphomycetes) 57 Pseudococcus sp. (Pseudococcidae) 54 2 Tortricidae, species of 50 1 Py ralidae, species of 44 2 Phycitinae, species of (Py ralidae) 39 Planococcus ficus (Signoret) (Pseudococcidae) 39 Pseudaonidia trilobitiformis (Green) (Diaspididae) 35 Planococcus minor (Maskell) (Pseudococcidae) 32 Lycaenidae, species of 29 Olethreutinae, species of (Tortricidae) 27 Tephritidae, species of 24 Ectomyelois sp. (P yralidae) 22 1 Crematogaster sp. (Formicidae) 21 Cryptophlebia sp. (Tortricidae) 21 2 **2.4 .**  **Pest Categorization: Identification of Quarantine Pests and Quarantine Pests Likely to**  **Follow the Pathway** We developed a list of pests potentially associated with pomegranate in India (Table 4) . The list identifies: (1) the presence or absence of the pests in the United States; (2) the references supporting an association between the pest and the host; (3) the plant organs where the pest can be found; (4) the U.S. quarantine status of the pests; and (5) the likelihood the pest would follow the pathway

into the continental United States through commercial shipments of pomegranate.Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 5 A quarantine pest is defined as “a pest of potential economic importance to the area endangered thereb y, but not yet present, or present, but not widely distributed and being officiall y controlled” ( IPPC, 200 7: ISPM #5 ). A pest is likely to follow the pathway

on p omegranate fruit if the pest is present in India, if the pest is associated with the fruit at the time of harvest, and if the pest remains with the fruit in viable form during harvesting, packing, and shipping procedures. The possibility that a quarantine pest following the pathway could establish and spread throughout the United States depends on the presence of appropriate climatic and ecological conditions, including protected areas (such as greenhouses), where the presence of primary or alternative host s and vectors are influential. **Table 4.** Pests associated with pomegranate , Punica granatum, anywhere in the world and present in India **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Arthropods**  **Acari :**  **Acaridae Tyrophagus putrescentiae**  (Schrank) IN , U.S. (CABI, 2007) PestID , 2007 N/A No N/A **Acari :**  **Eriophyidae Eriophyes granati**  (Canestrini & Massalongo) Sy n: Aceria granati IN(Prasad,

1971); U.S. (CA, FL) (Baker et al
. , 1996) Davis et al
. , 1982;

Dhooria and Bhullar, 2003 L (Baker et al
. ,

1996; Jeppson et al
. , 1975) No No
**Acari :**  **Tenuipalpidae Brevipalpus phoenicis**  Geijskes Sy n: B. papayensis IN(Prasad,

1971); U.S. (FL) (CABI, 2007) Maes, 2004 N/A No N/A **Tenuipalpus granati** Sayed IN(Meena et al
. , 2004) Jeppson et al
. ,

1975 St , L , F (Jeppson

et al
. , 1975) Yes Yes **Tenuipalpus punicae** Pritchard & Baker IN(Prasad, 1971) Dhooria, 2003;

Jeppson et al
. , 1975 St , L , F (del

Rivero, 1991; Jeppson et al
. , 1975) Yes Yes

1 Plant parts: Bk = Bark, F = fruit, I = inflorescence, L = leaf, Sh = Shoot, S t = Stem , R = root , Tr = Trunk 2 N/A= Not Applicable. Even if non
- quarantine pests are able to follow the pathway, phytosanitary measures against these pests would not be justified considering the pest already occurs in the United States. Therefore, information on plant part association and whether the pest is “likely to follow the pathway” is not needed for non
- quarantine pests.Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 6 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Acari**  **: Tetranychidae**  **Eutetranychus orientalis** (Klein) Sy n: E
. monodi ; E
. sudanicus ; E
. anneckei Anychus orientalis ; A
. ricini IN(Bolland et al
. , 1998) Bolland et al
. ,

1998; Kumawat and Singh, 2002 L (Hill, 1983) Yes No

**Oligonychus coffeae** Nietner Sy n: Acarus coffeae ; Paratetranychus bioculatus IN , U.S. (Bolland et al
. , 1998) Bolland et al
. ,

1998 N/A No N/A
**Oligonychus mangiferus** (Rahman & Sapra) IN(Bolland et al
. , 1998) Bolland et al
. ,

1998 L (Jeppson et al
. ,

1975; Waite, 2002) Yes No **Oligonychus punicae** (Hirst) Sy n: Paratetranychus punicae IN , U.S. (Bolland et al
. , 1998) Bolland et al
. ,

1998; Jeppson et al
. , 1975 N/A No N/A **Tetranychus kanzawai**  Kishida IN(Bolland et al
. , 1998); U.S. (Bolland et al
. , 1998) Bolland et al
. ,

1998 N/A No N/A **Tetranychus urticae** Koch Sy n: T. aduncus, T. arabicus, T. cinnabarinus, T. ricinus, T. telarius IN , U.S. (Bolland et al
. , 1998) Bolland et al
. ,

1998 N/A No N/A
**Coleoptera :**  **Cerambycidae Celosterna spinator**  Fabricius IN(Sheikh, 2006) Sheikh, 2006 St, Tr (Sheikh, 2006) Yes No **Cerosterna scabrator** Fabr. IN(Biradar et al
. , 2005) Biradar et al
. ,

2005 St (Biradar et al
. ,

2005) Yes No **Olenecamptus bilobus** F. IN(Hill, 1983) Hill, 1983; Patil et al
. , 2002 Tr, St (Hill, 1983;

Patil et al
. , 2002) Yes No **Coleoptera :**  **Curculionidae Hypera postica**  (G yl lenhal) Sy n: H. brunnipennis IN , U.S. (CABI,

2007) PestID , 2007 N/A No N/A **Myllocerus discolor** Boheman IN(Srivastava, 1997) Srivastava, 1997 Sh , L, I (Srivastava, 1997) Yes NoPest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 7 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Myllocerus lactivirens**  Marshall IN(Sheikh, 2006) Sheikh, 2006 R , L (Sheikh, 2006) Yes No **Myllocerus maculosus** (Pesbrocher) IN(Butani and Verma, 1981) Nair, 1975 L (Nair, 1975) Yes No **Myllocerus sp.**  IN(Hill, 1983) Hill, 1983 L (Hill, 1983) Yes 3 No **Myllocerus undecimpustulatus**  Desbrocher IN(Sheikh, 2006) Sheikh, 2006 R , L (Sheikh, 2006) Yes No **Coleoptera :**  **Dryophthoridae Sitophilus oryzae**  (Linneaus) IN(CABI, 2007); U.S. (Arnett Jr., 1997) PestID , 2007 N/A No N/A **Coleoptera :**  **Scarabaeidae Anomala dimidiata**  Hope IN(Sheikh, 2006) Sheikh, 2006 R , L (Sheikh, 2006) Yes No **Anomala sp.**  IN(Hill, 1983) 4 ; U.S. (Arnett Jr. et al
. , 2002; Krauth, 2002) Hill, 1983 N/A No N/A
**Holotrichia insularis** Brenske IN(Butani and Verma, 1981) Nair, 1975 L (Butani and Verma, 1981; Nair, 1975) Yes No **Hoplasoma sexmaculata** Hope IN(Sheikh, 2006) Sheikh, 2006 R , L (Sheikh, 2006) Yes No **Mimastra cyanura** Hope IN (Sheikh, 2006) Sheikh, 2006 R , L (Sheikh, 2006) Yes No **Coleoptera :**  **Scolytidae Euwallacea fornicatus**  Sy n: Xyleborus fornicatus IN , U.S. (CA, FL) (CABI, 2007) CABI, 2007; Mote

and Tambe, 2000 St (CABI, 2007) [Yes] 5 No **Xyleborus dispar** Fabricius IN , U.S. (Cognato, 2007) Cognato, 2007 N/A No N/A

3 The only species of Myllocerus in the United States is M. hilleri . Because M. hilleri does not appear to occur in India, the Myllocerus sp. taxon that attacks pomegranate in India is most likely a different species from that in the U.S. Thus this taxon is a quarantine pest. 4 At least 33 species of Anomala occur in the United States (Arnett Jr., 1997). It is unknown if this particular taxon on pomegranate occurs in the United States. 5 This pest has a limited distribution in the United States and is considered a quarantine pest (Courneya, 2006).Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 8 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Xyleborus perforans**  (Wollaston) Sy n: X. kraatzi IN(Jagginavar

and Naik, 2005) Jagginavar and

Naik, 2002, 2005) St (Wood) (CABI,

2007); R (Jagginavar and Naik, 2002) Yes No **Diptera : Cecidomyiidae**  **Aphidoletes aphidimyza** (Rondani) IN , U.S. (CABI, 2007) PestID, 2007 N/A 6 No N/A **Diptera : Tephritidae**  **Bactrocera carambolae** IN(CABI, 2007) CABI, 2007 F (Waite, 2002) Yes Yes **Bactrocera dorsalis** (Hendel) Sy n: Dacus dorsalis IN (Srivastava,

1997) CABI, 2007;

Sheikh, 2006; PestID, 2007 F (CABI, 2007) Yes Yes **Bactrocera zonata** Sy n: Dacus zonatus IN(CABI, 2007;

Srivastava, 1997) CABI, 2007;

Sheikh, 2006 F (Waite, 2002) Yes Yes **Dacus sp.**  **7** IN(Hill, 1983) Hill, 1983 F (Hill, 1983) Yes Yes **Hemiptera : Aleyrodidae**  **Acaudaleyrodes rachipora** (Singh) Sy n: A. citri IN(Evans, 2007) CABI, 2007; Meeta et al, 1999 L (Sundararaj and

Murugesan, 1996; Verma, 2006) Yes No **Aleurocanthus woglumi** Ashby Sy n: Aleurodes woglumi IN(CABI, 2007)

U.S. (AZ, CA, FL, MS, TX) (Evans, 2007) Hill, 1983; Mound

and Halsey , 1978 L (Mound and

Halsey , 1978) [Yes] 5 No **Aleurodicus dispersus** Russell IN(Evans, 2007); U.S. (FL) (CABI, 2007) Mani et al
. , 2003 L, F (CABI, 2007); L (Mani et al, 2003) [Yes] 5 No 8 **Aleurothrixus floccosus** (Maskell) IN(CABI, 2007); U.S. (CA FL LA TX) (Evans, 2007) Ulusoy et al
. , 2003 N/A No N/A
**Dialeurodes citri** (Ashmead) IN , U.S.(CABI, 2007) CABI, 2007;

Mound and Halsey , 1978 N/A No N/A

6 This species is not a true plant pest, because it feeds on aphids and other species (CABI, 2007). 7 In 1989, R.A.I. Drew

revised the Dacinae of Australasian and Oceanian regions and placed most Dacus into the genus Bactrocera . Post 1989, references to Dacus spp. would be species that are highly unlikely to attack pomegranate. References to Dacus spp. before 1989 would include all of the specie s that are now placed in the genus Bactrocera (many of which might attack pomegranate) . 8 primarily a foliage pest and that fruit for consumption is unlikely to be a good pathway for movement into the United StatesPest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 9 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Siphoninus**  **phillyreae** (Haliday) Sy n: S. finitimus, S. granati IN , U.S. (CA) (CABI, 2007) CABI, 2007;

Klein Koch and Waterhouse, 2000; Mound and Halsey , 1978 Sh (Sheikh,

2006); L (CABI, 2007) [Yes] 5 No

**Trialeurodes vaporariorum**  Westwood IN , U.S. (CABI, 2007) Vergara and

Castro, 2006 N/A No N/A **Hemiptera : Aphididae**  **Aphis craccivora** Koch IN(Anjaneya Murthy and Regupathy, 1989); U.S. (CABI, 2007) Blackman and

Eastop, 2000 N/A No N/A
**Aphis fabae** Scopoli Sy n: A. rumicis IN , U.S.(CABI,

2007 Blackman and

Eastop, 2000 N/A No N/A **Aphis gossypii** Glover IN , U.S. (CABI, 2007) Blackman and

Eastop, 2000; CABI, 2007 N/A No N/A **Aphis punicae** Passerini IN(Hill, 1983) Blackman and Eastop, 2000; Millar, 1994 L (Hill, 1987) Yes No **Aphis spiraecola** Patch Sy n: A. citricola IN , U.S. (CABI,

2007) Blackman and

Eastop, 2000; Maes, 2004 N/A No N/A **Aulacorthum circumflexum**  Kaltenbach Sy n: Neomyzus circumflexus IN(CABI, 2007); U.S. (USDA, 2007b) Blackman and

Eastop, 2000 N/A No N/A
**Brachycaudus helichrysi** Kaltenbach IN , U.S. (CABI, 2007) Blackman and

Eastop, 2000 N/A No N/A **Myzus ornatus** Laing IN(Blackman and Eastop, 2000); U.S. (Leonard et al
. , 1971) Blackman and

Eastop, 2000 N/A No N/A
**Myzus persicae** Sulzer Sy n: Aphis persicae IN , U.S. (CABI,

2007) Blackman and

Eastop, 2000; CABI, 2007 N/A No N/APest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 10 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Shivaphis celti**  Das (Aphididae) IN(Bhagat, 1982; Raychaudhuri et al
. , 1981); U.S. (FL & GA) (Halbert and Choate, 2003) PestID , 2007 N/A No N/A

**Toxoptera aurantii** Boyer de Fonscolombe IN , U.S. (CABI, 2007) Blackman and

Eastop, 2000 N/A No N/A **Hemiptera : Coccidae**  **Ceroplastes floridensis** Comstock Sy n: Cerostegia floridensis ; Paracerostegia floridensis IN(CABI, 2007); U.S. (Ben
- Dov et al
. , 2007) Ben - Dov et al
. ,

2007; CABI, 2007 N/A No N/A

**Ceroplastes pseudoceriferus**  Green IN(Ben
- Dov et al
. , 2007) Ben - Dov et al
. ,

2007 Sh , L (Ali, 1978) Yes No **Ceroplastes rubens** Maskell IN , U.S. (FL) (Ben
- Dov et al
. , 2007) Ben - Dov et al
. ,

2007 St , L , F (CABI,

2007) [Yes] 5 Yes 9 **Ceroplastes rusci** (Linnaeus) IN(Butani, 1993); US (FL) (Ben
- Dov et al
. , 2007) Ben - Dov et al
. ,

2007 St , L , F (CABI,

2007) [Yes] 5 Yes **Coccus hesperidum** Linnaeus IN(Butani, 1993); U.S. (CABI, 2007) Ben - Dov et al
. ,

2007 N/A No N/A **Parasaissetia nigra** Nietner Sy n: Lecanium signatum ; Saissetia nigra ; S. persea e IN(Ben
- Dov et al
. , 2007); U.S. (CABI, 2007) Shevale and

Kaulgud, 1998 N/A No N/A

**Parthenolecanium corni** (Bouché) IN , U.S. (Ben
Dov et al
. , 2007) Ben - Dov et al
. ,

2007 N/A No N/A **Parthenolecanium persicae**  (Fabricius) Sy n: Coccus elongatus IN , U.S. (Ben
Dov et al
. , 2007) CABI, 2007 N/A No N/A

9 This pest has been intercepted 4527 times, 22 of which were on fruit (PestID, 2007).Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 11 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Pulvinaria psidii**  Maskell Sy n: Chloropulvinaria psidii IN , U.S. (Ben
Dov et al
. , 2007) Ben - Dov et al
. ,

2007 N/A No N/A **Saissetia coffeae** Walker Sy n: S. hemisphaerica (Targioni) IN; U.S. (CABI, 2007) Ben - Dov et al
. ,

2007 N/A No N/A **Saissetia miranda** (Cockerell & Parrott in Cockerell) IN , U.S. (Ben
Dov et al
. , 2007) Ben - Dov et al
. ,

2007 N/A No N/A **Saissetia oleae** (Olivier) Sy n: Coccus oleae IN , U.S. (Ben
Dov et al
. , 2007) Ben - Dov et al
. ,

2007; CABI, 2007 N/A No N/A **Hemiptera : Coreidae**  **Leptoglossus gonagra** Fabricius Sy n: L. australis ; Theognis gonagra IN(CABI, 2007; Jhala et al
. , 2003); U.S. (Froeschner, 1998) DEWR, 2007;

Jhala et al
. , 2003; Maes, 2004 N/A No N/A
**Leptoglossus me**  **mbranaceus** (Fabricius) IN(Jadhav et al
. , 1976) Patil et al
. , 2002 F (Patil et al
. , 2002) 19 Yes No 10 **Hemiptera : Diaspididae**  **Abgrallaspis cyanophylli** (Signoret) IN(Ben
- Dov et al
. , 2007); U.S. (BMESD, 2003; Kessing and Mau, 1993) Maes, 2004 N/A No N/A
**Andaspis hawaiiensis** (Maskell) Sy n: Lepidosaphes hawaiiensis IN(Sheikh, 2006); U.S. (FL) (Ben
- Dov et al
. , 2007) Sheikh, 2006 N/A No N/A **Andaspis leucophleae** Rao in Rao & Ferris IN(Ben
- Dov et al
. , 2007) Ben - Dov et al
. ,

2007 St epidermis

(Ben
- Dov et al
. , 2007); L (Sheikh, 2006) 11 Yes No
**Aonidiella aurantii** Maskell IN , U.S. (Ben
Dov et al
. , 2007) Maes, 2004 N/A No N/A

10 Because coreids in general are large and nymphs are gregarious (Borror et al
. , 1989), these pests should be readily seen during harvest and culling, and eliminated from the pathway. Furthermore, Leptoglossus membranaceus can cause pomegranate fruit to drop prematurely (Patil et al
. , 2002), making it unlikely that this pest will follow the pathway on fruit. 11 Due to limited information available on this taxon, we based the plant part association on the congen er A. hawaiiensis , which also attacks pomegranate.Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 12 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Aonidiella**  **orientalis** (Newstead) Sy n: Aspidiotus orientalis IN(CABI, 2007); U.S. (Ben
- Dov et al
. , 2007) Ben - Dov et al
. ,

2007 N/A No N/A **Aspidiotus destructor** Signoret Sy n: A. transparens ; A. watanabei ; Temnaspidiotus destructor IN; U.S. (CABI, 2007) Bruner et al
. , 1945 N/A No N/A

**Aspidiotus nerii** Bouche Sy n: A. unipectinatus, A
. hederae IN(Butani, 1993); U.S. (Ben
- Dov et al
. , 2007; CABI, 2007) Ben - Dov et al
. ,

2007; Hill, 1987 N/A No N/A
**Chrysomphalus aonidum** Linnaeus Sy n: C
. ficus, Aonidiella ficorum , Aspidiotus aonidum IN(Ben
- Dov et al
. , 2007); U.S. (Ben
- Dov et al
. , 2007; CABI, 2007) Ben - Dov et al
. ,

2007 N/A No N/A
**Diaspidiotus perniciosus** (Comstock) Cockerell; Danzig. Sy n: Quadraspidiotus pernicious (Comstock) IN , U.S.(Ben
Dov et al
. , 2007; CABI, 2007) Vergara and

Castro, 2006 N/A No N/A
**Duplaspidiotus tesseratus** (de Charmo y) IN(Ben
- Dov et al
. , 2007); U.S. (FL) (Dekle and Kuitert, 1975) Sheikh, 2006 N/A No N/A **Hemaspidoproctus cinereus**  (Green) Sy n: Aspidoproctus cinerea IN(Sheikh, 2006) Nair, 1975;

Sheikh, 2006 L (Sheikh, 2006) Yes NoPest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 13 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Hemiberlesia lataniae**  Signoret Sy n: Aspidiotus lataniae, Diaspidiotus lataniae , Euaspidiotus lataniae IN(Ben
- Dov et al
. , 2007); U.S. (Ben
- Dov et al
. , 2007; CABI, 2007) Ben - Dov et al
. ,

2007 N/A No N/A
**Hemiberlesia punicae** (Signoret) 12 IN(Sheikh, 2006) Sheikh, 2006 L (Sheikh, 2006) Yes No **Hemiberlesia rapax** (Comstock) IN(Butani, 1993); U.S. (Ben
- Dov et al
. , 2007) Johnson and Lyon,

1991 N/A No N/A **Lepidosaphes ulmi** (Linnaeus) IN , U.S. (Ben
Dov et al
. , 2007) Ben - Dov et al
. ,

2007 N/A No N/A **Lindingaspis greeni** (Brain & Kell y) IN(Sheikh, 2006) Sheikh, 2006 L (Sheikh, 2006) Yes No **Lindingaspis rossi** (Maskell) Sy n: Aspidiotus rossi , Chrysomphalus rossi IN(Hill, 1983; Sheikh, 2006); U.S. (CA) (Ben
Dov et al
. , 2007) Hill, 1983 N/A No N/A
**Parlatoria oleae** (Colvée) IN(Hill, 1983; Sheikh, 2006); U.S. (Ben
- Dov et al
. , 2007) Ben - Dov et al
. ,

2007 N/A No N/A **Pinnaspis buxi**  (Bouche) IN , U.S. (Ben
Dov et al
. , 2007) Ben - Dov et al
. ,

2007 N/A No N/A **Pinnaspis strachani** Cooley Sy n: P. gossypii ; P. marchali; P. temporaria IN , (Ben
- Dov et al
. , 2007); U.S. (Ben
- Dov et al
. , 2007; CABI, 2007) Ben - Dov et al
. ,

2007 N/A No N/A
**Pinnaspis theae** (Maskell) Sy n: Hemichionaspis theae IN(Ben
- Dov et al
. , 2007) Sheikh, 2006 L (Ben
- Dov et al
. , 2007; Sheikh, 2006) Yes No **Pseudaonidia trilobitiformis**  Green Sy n: Aspidiotus trilobitiformis IN(Ben
- Dov et al
. , 2007); U.S.FL (Ben
Dov et al
. , 2007; Coile, 2000) Ben - Dov et al
. ,

2007 L (Gallego and

Velez, 1992; Hill, 1983); F PestID , 2007) [Yes] 5 Yes

12 This pest name does not appear anywhere else in the literature, including ScaleNet (Ben
- Dov et al
. , 2007), abstracting databases (e.g., Cab Abstracts, Agricola), Google, or other texts of pests in India (Butani, 1993; Nair, 1975; Srivastava, 1997). Thus, this pest was likely a misidentification of H. rapax or another diaspid scale.Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 14 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Selenaspidus articulatus**  Morgan IN(Ben
- Dov et al
. , 2007); U.S. (CA & FL) (Ben
- Dov et al
. , 2007; CABI, 2007) Maes, 2004 N/A No N/A

**Howardia biclavis** (Comstock) IN(Ben
- Dov and Hodgson, 1997); U.S. (Ben
- Dov et al
. , 2007) Ben - Dov et al
. ,

2007 N/A No N/A **Hemiptera : Kerridae**  **Kerria lacca (Kerr)** IN(Ben
- Dov et al
. , 2007) Ben - Dov et al
. ,

2007 St (Chen et al
. ,

2004) Yes No **Hemiptera : Margarodidae**  **Drosicha dalbergiae** IN(Ben
- Dov et al
. , 2007) CABI, 2007 R (Masoodi et al
. , 1988); Twigs (Vijay et al
. , 2000a); F (Rawat et al
. , 1989) Yes Yes
**Drosicha mangiferae** (Stebbing) IN(Ben
- Dov et al
. , 2007) Hill, 1983;

Srivastava, 1997 Sh , L, I

(Srivastava, 1997) Yes No **Icerya aegyptiaca** (Douglas) IN(CABI, 2007) Ben Dov et al, 2007; CABI, 2007 St , L , F (CABI,

2007) Yes Yes **Icerya purchasi** Maskell IN; U.S. (CABI, 2007) CABI, 2007;

Johnson and Lyon, 1991 N/A No N/A **Icerya seychellarum**  Westwood Sy n: Dorthesia seychellarum ; I. okadae IN(CABI, 2007) Ben - Dov et al
. , 2007; CABI, 2007 St , L (CABI,

2007); I (USDA, 1982); F (USDA, 1982; PestID , 2007) Yes Yes
**Hemiptera : Pentatomidae**  **Jurtina indica** D. IN(Nair, 1975) Nair, 1975 F (Nair, 1975) Yes No 13 **Halyomorpha picus** Fabricius IN(Nair, 1975) Nair, 1975 F (Nair, 1975) Yes No 1 2 **Hemiptera : Pseudococcidae**  **Dysmicoccus brevipes** (Cockerell) IN , U.S. (Ben
Dov et al
. , 2007) PestID , 2007 N/A No N/A

13 Pentatomids are medium
- sized insects, sometimos gregarious, that should be readily seen and elminated from the pathway during harvest and post
- harvest.Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 15 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Dysmicoccus neobrevipes**  Beardsley IN(Ben
- Dov et al
. , 2007) Ben - Dov et al
. ,

2007 R, St, L , I , F

(PestID, 2007) Yes Yes **Ferrisia malvastra** (McDaniel) Sy n: F. consobrina IN(Ben
- Dov et

al
. , 2007) ; US Ben - Dov et al
. ,

2007 F (Shevale, 1994);

[ St , and L (CABI, 2007)] 14 No Yes **Ferrisia virgata** (Cockerell) Sy n: Pseudococcus virgatus IN(Hill, 1983); U.S. (CABI, 2007) Ben - Dov et al
. ,

2007; Hill, 1983 N/A No N/A
**Maconellicoccus hirsutus** Greene Sy n: Paracoccus pasaniae; M. perforatus ; M. pasaniae IN , U.S. (CA, FL) (CABI, 2007) Ben - Dov et al
. ,

2007 St , L , I , F (CABI,

2007) [Yes] 5 Yes
**Nipaecoccus viridis** (Newstead) IN(Ben
- Dov et al
. , 2007) Kulkarni et al
. ,

2003; Shevale, 1994 R , St , L , F (Ben
Dov et al
. , 2007); F (Shevale, 1994 **)** Yes Yes **Planococcus angkorensis** (Takahashi) Sy n: P. dorsospinus Ezzat & Mcconnell IN(Ben
- Dov et al
. , 2007) Ben - Dov et al
. ,

2007 [ L (Hill, 1983), I

(Williams and Watson, 1988), and F (Murray , 1976; PestID , 2007)] 15 Yes Yes

**Planococcus citri** Risso IN , U.S. (Ben
Dov et al
. , 2007) Ben - Dov et al
. ,

2007; Klein Koch and Waterhouse, 2000 N/A No N/A **Planococcus ficus** (Signoret) IN , U.S. (Ben
Dov et al
. , 2007) Ben - Dov et al
. ,

2007; CABI, 2007 N/A No N/A

14 Ferrisia malvastra attacks host fruit (Shevale, 1994), but we assumed that it could also attack stem s and leaves, like its congener F. virgata , with which it is often confused (CABI, 2007). 15 Due to limited information on this pest, we assumed its association with host plant organs was similar to other Planococcus included in this pest list.Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 16 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Planococcus lilacinus**  (Cockerell) Sy n: Pseudococcus crotonis IN(CABI, 2007) Ben - Dov et al
. , 2007; Hill, 1983 L (Hill, 1983), F

(Nair, 1975) Yes Yes
**Planococcus minor** Maskell Sy n: P. pacificus IN(Ben
- Dov et

al
. , 2007) PestID , 2007 16 L (Aguiar
Menezes et al
. , 2002; PestID , 2007); I (Williams and Watson, 1988); F ( PestID , 2007) Yes No 17

**Pseudococcus cryptus** Hempel Sy n: P. citriculus IN(Ben
- Dov,

1994) Ben - Dov et al
. ,

2007 F (Williams and

Watson, 1988) ; L (Avidov and Harpaz, 1969); R (Ben
- Dov et al
. , 2007); Sh (Ben
Dov et al
. , 2007; Williams and Watson, 1988) Yes Yes

**Pseudococcus longispinus** (Targioni Tozzetti) Sy n: P. adonidum IN(CABI,

2007); U.S. (McKenzie, 1967) Ben - Dov et al
. ,

2007 N/A No N/A **Isoptera : Termitidae**  **Odontotermes obesus** (Rambur) IN(Sheikh, 2006) Sheikh, 2006 Bk (Paul et al
. , 1993) Yes No **Trinervitermes biformis** (Wasmann) IN(Patel and Patel, 1952) Patel and Patel,

1952 R (Butani and

Varma, 1976) No Yes **Lepidoptera : Arctiidae**  **Creatonotus gangis** (Linnaeus) IN(Sheikh, 2006) Robinson et al
. ,

2001; Sheikh, 2006 L (Sheikh, 2006) Yes No **Lepidoptera :**  **Cosmopterigidae Anatrachyntis simplex**  Walsingham Sy n: Pyroderces simplex IN(Heckford,

2004; Robinson et al
. , 2007) Heckford, 2004;

Robinson et al
. , 2001 F (Heckford,

2004) Yes No 18

16 All interceptions on passenger baggage; no other evidence of host association could be found in the scientific literature. 17 Given the only weak evidence for host association (e.g., interceptions on passenger baggage), we conclude that pomegranate fruit for consumption is unlikely to be a good pathway for movement into the United States. 18 Anatrachyntis simplex is unlikely to follow the pathway on fresh, commercial quality pomegranate fruit because it feeds on already damaged or rotten plant tissue (Heckford, 200 4).Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 17 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Lepidoptera**  **: Cossidae**  **Zeuzera coffeae** Neitn. IN(Hill, 1983) Hill, 1983; Robinson et al
. , 2007 St (Hill, 1983;

Sheikh, 2006) Yes No **Lepidoptera :**  **Crambidae Conogethes punctiferalis**  (Guenée) Sy n: Dichocrocis punctiferalis (CABI, 2007) IN(CABI, 2007; Hill, 1983) CABI, 2007; Hill,

1983 L (Ramakrishna

Ayyar, 1936); St (CABI, 2007), and F (CABI, 2007; Hill, 1983) Yes Yes
**Lepidoptera :**  **Gelechiidae Phthorimaea operculella**  (Zeller) IN , U.S. (CABI, 2007) USDA, 2007a N/A No N/A **Lepidoptera :**  **Lasiocampidae Streblote siva**  (Lef.) IN(Robinson et al
. , 2007) Robinson et al
. ,

2007 L (Parui, 1971) Yes No **Suana concolor** (Walker) IN(Robinson et al
. , 2007) Robinson et al
. ,

2001; Robinson et al
. , 2007 L (Hutson and

Jardine, 1923) Yes No **Trabala vishnou** (Lefevere) IN(Mani et al
. , 2001) Mani et al
. , 2001;

Robinson et al
. , 2001 L (Hill, 1987) Yes No **Lepidoptera :**  **Limacodidae Latoia lepida**  (Cram.) IN(Hill, 1983) Hill, 1983 L (Hill, 1987) Yes No **Parasa lepida**  Cramer IN(CABI, 2007) Robinson et al
. , 2007 L (Nair, 1975;

Robinson et al
. , 2001) Yes No **Thosea sinensis** IN(Robinson et al
. , 2007) CABI, 2007;

Robinson et al
. , 2007 L (Nair, 1975) Yes No **Thosea unifascia** IN(Robinson et al
. , 2007) Robinson et al
. ,

2007 L (I saac, 1925;

Robinson et al
. , 2001) Yes NoPest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 18 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Lepidoptera**  **: Lycaenidae**  **Deudorix epijarbas** (Moore) IN(Hill, 1983) CABI, 2007; Hill, 1983 F (Hill, 1983) Yes Yes **Deudorix isocrates** Fabricius Sy n: Virachola isocrates IN(CABI, 2007;

Hill, 1983) Morton, 1987;

Patil et al
. , 2002 I , F (Morton,

1987), F (Patil et al
. , 2002) Yes Yes 19 **Iraota timoleon** Stoll IN(Savela, 2007) Savela, 2007 St , L , I Yes No **Lepidoptera :**  **Lymantriidae Euproctis flava**  (Bremer) IN(Sheikh, 2006) Sheikh, 2006 L (Sheikh, 2006) Yes No **Euproctis fraterna** (Moore) IN(Robinson et al
. , 2007; Sheikh, 2006) Sheikh, 2006 L (Cantelo et al
. , 1965; Robinson et al
. , 2001) Yes No **Euproctis lunata** Walker IN(Subramanian and Krishnamurthy, 2002) Sheikh, 2006 L (Sheikh, 2006) Yes No **Euproctis scintillans** Walker Sy n : Porthesia scintillans , Somena scintillans IN(Robinson et al
. , 2007; Sheikh, 2006) Sheikh, 2006 L, I (Robinson et al
. , 2001) Yes No
**Euproctis sp.**  IN(Hill, 1983) Hill, 1983 L (Hill, 1987) Yes No **Euproctis subflava** Bremer IN(Robinson et al
. , 2007) Robinson et al
. ,

2007 L (Ahn et al
. ,

1989; Robinson et al
. , 2001) Yes No **Lymantria ampla** (Walker) IN(Srivastava, 1997) Srivastava, 1997 L (Srivastava, 1997) Yes No **Lymantria beatrix** Stoll IN(Robinson et al
. , 2007) Robinson et al
. ,

2001; Robinson et al
. , 2007 L (Srivastava,

1997) Yes No **Olene mendosa** Hubner Sy n: Dasychira mendosa IN(Robinson et

al
. , 2007) Cantelo et al
. ,

1965; Robinson et al
. , 2001 L (Cantelo et al
. ,

1965 Yes No **Lepidoptera :**  **Metarbelidae Indarbela dea**  Swinhoe IN(CABI, 2007) CABI, 2007 St , Bk (CABI, 2007) Yes No

19 Deudorix isocrates is one of the most important and destructive pests of pomegranate in India (Nair, 1975 ). It is an internal pest. This pest may only sometimes be noticed during harvest and post
- harvest processing (due to entry holes and excreta) (Patil et al
. , 2002), particularly if it enters the fruit from the calyx end. This pest pupates inside of the fruit (Nair, 1975) .Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 19 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Indarbela quadrinotata**  (Walker, 1856) IN(NHM, 2006) Patil et al
. , 2002; Robinson et al
. , 2001 Bk (Sen
- Sarma,

1987); Yes No **Indarbela sp.**  IN(Hill, 1983) Hill, 1983 Bk (Hill, 1983) Yes No **Indarbela tetraonis** (Moore) IN(Butani and Verma, 1981; NHM, 2006) Patil et al
. , 2002;

Robinson et al
. , 2001 St , Bk (Butani

and Verma, 1981) Yes No **Lepidoptera :**  **Noctuidae Achaea janata**  (L .) IN (CABI, 2007) Robinson et al
. , 2001 L , F (Hill, 1994;

Robinson et al
. , 2001; Sheikh, 2006) Yes No 20 **Arcyophora dentula** (Lederer) IN(Sheikh, 2006) Sheikh, 2006 L (Sheikh, 2006) Yes No **Dysgonia algira** (Linnaeus) IN(Robinson et al
. , 2007) CABI, 2007;

Robinson et al
. , 2007 L (Robinson et

al
. , 2007) Yes No **Ercheia diversipennis** Walker IN(Robinson et al
. , 2001; Robinson et al
. , 2007) Robinson et al
. ,

2007 F (Robinson et al
. ,

2001; Susainathan, 1924) Yes No 21 **Eudocima fullonia** Clerck Sy n: Ophideres fullonia ; O. fullonica ; O threis fullonia ; O. fullonica IN(Hill, 1983) CABI, 2007 L (CABI, 2007); F (CABI, 2007; Fors yth, 1966; Hill, 1983) Yes No 22

**Eudocima homaena** Hubner Sy n: Othreis homaena IN(CABI, 2007;

Robinson et al
. , 2007) Bhumannavar and

Viraktamath, 2001; CABI, 2007; Robinson et al
. , 2007 F (Bhumannavar

and Viraktamath, 2001) Yes No 23

**Eudocima materna** Linnaeus Sy n: Othreis materna IN(CABI, 2007;

Robinson et al
. , 2007) CABI, 2007;

Robinson et al
. , 2007 L (Mohite et al
. ,

2004); F (Fay

and Halfpapp, 2006; Fors yth, 1966; Zhang, 1994) Yes No 24

20 Adults of A. janata feed on the fruit of pomegranate, while larvae feed on the leaves (Sheikh, 2006). Thus, A. janata is not expected to follow

the pathway because adults are relatively large and mobile. 21 Because only adults feed on fruit (Susainathan, 1924), this pest is not expected to follow the pathway on fresh pomegranates. 22 Only adults are associated with fruit (CABI, 2007; Hill, 1983) and consequently are not expected to follow the pathway. 23 Because only adults are associated with fruit (Bhumannavar and Viraktamath, 2001), this pest is not expected to follow the pathway.Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 20 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Helicoverpa armigera**  (Hübner) Sy n: Heliothis armigera IN(Teggelli et

al
. , 2002) Teggelli et al
. ,

2002 Sh , L , I (CABI,

2007); F (Teggelli et al
. , 2002; CABI, 2007) Yes No 25 **Ophiusa tirhaca** Cramer IN(Robinson et al
. , 2007) Robinson et al
. ,

2007 L (Robinson et

al
. , 2001) Yes No **Lepidoptera :**  **Nolidae Arcyophora dentula**  Led. IN(Anonym ous, 1941) Robinson et al
. ,

2001; Robinson et al
. , 2007) L (Anony mous, 1941) Yes No **Lepidoptera :**  **Oecophoridae Stathmopoda auriferella**  Walker IN(Robinson et al
. , 2007) Robinson et al
. ,

2007 F (Park et al
. ,

1994) Yes No 26 **Lepidoptera :**  **Psychidae Acanthopsyche cana**  Hampson IN(Robinson et al
. , 2007) Robinson et al
. ,

2007 L (Robinson et

al
. , 2007) Yes No **Acanthopsyche sp**  **.** 27 IN(Robinson et al
. , 2001; Sheikh, 2006) Sheikh, 2006 L (Sheikh, 2006) Yes No **Eumeta crameri** Westwood IN(Hill, 1983; Robinson et al
. , 2007) Hill, 1983; Patil et

al
. , 2002 Bk (Robinson et

al
. , 2001); L (Hill, 1983; Patil et al
. , 2002; Robinson et al
. , 2001) Yes No

**Pteroma sp**  . IN(Nair, 1975) Nair, 1975 L (Nair, 1975) Yes No **Lepidoptera :**  **Py ralidae**  **Apomyelois ceratoniae** Zellar Sy n: Ectomyelois ceratoniae IN(Singh, 1991); U.S. (Robinson et al
. , 2007) CABI, 2007; Patil

et al
. , 2002; Robinson et al
. , 2007 N/A No N/A
**Cadra cautella** (Walker) Sy n: Ephestia cautella IN(CABI,

2007); U.S. (Arnett Jr., 2000) Carter, 1984;

Robinson et al
. , 2007 N/A No N/A

24 Because only the adult life form is a ssociated with fruits (Fay and Halfpapp, 2006), this moth is not expected to follow the pathway. 25 On pomegranate, H. armigera inserts its head and anterior parts into the fruit (Teggelli et al
. , 2002). Therefore, this pest should be readily noticed and el iminated from the pathway during harvest and post
- harvest processing. 26 Stathmopoda auriferilla, like many members of the family Oecophoridae **,** generally only feeds on decaying plant material. It appears to only be associated with rotten fruit of pomegranat e (Robinson et al
. , 2001) and would not be associated with fresh fruit. 27 Sheikh (2006) reports that the bag worm “ Acantho psyche ” attacks pomegranate in India. We considered the extra space to be a typographical error and that the true name was Acanthops yche , which is a genus of bagworms.Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 21 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Cryptoblabes gnidiella**  Millière Sy n: Albinia casazzar ; A. gnidiella ; Ephestia gnidiella IN(CABI, 2007) CABI, 2007; Robinson et al
. , 2007 St , L , F (CABI,

2007); F (Carter, 1984) Yes Yes 28
**Dichocrocis megillalis** Walker IN(CABI, 2007) Robinson et al
. , 2007 F (Cantelo et al
. ,

1965; Robinson et al
. , 2001) Yes Yes 29 **Ephestia elutella** Hubner IN(Robinson et al
. , 2007); U.S. (NC) (CABI, 2007) Robinson et al
. ,

2007 N/A No N/A **Euzophera bigella** Zeller Sy n: E. punicaeella (Pasuki, 1975) IN (Robinson et al
. , 2007) Robinson et al
. ,

2007 L , F (Nazeer

Ahmad, 1942; Robinson et al
. , 2001); F (Robinson et al
. , 2001) Yes Yes 30

**Paralipsa gularis** (Zeller) Sy n: Aphomia gularis IN(Butani,

1978; Joseph and Oommen, 1961; Robinson et al
. , 2001); U.S. (Arnett Jr., 1997 **)** Robinson et al
. ,

2007 N/A No N/A

**Plodia interpunctella** (Hubner) IN , U.S. (CABI, 2007) Robinson et al
. ,

2007 N/A No N/A **Pyralis farinalis** Li nnaeus IN(Robinson et al
. , 2007); U.S. (Arnett Jr., 2000) Robinson et al
. ,

2007 N/A No N/A **Lepidoptera :**  **Saturniidae Actias selene**  Hubner IN(NHM, 2006) Robinson et al
. , 2001 L (Robinson et

al
. , 2001; Viqar et al
. , 2005) Yes No **Attacus atlas** Linnaeus IN(Robinson et al
. , 2007) Robinson et al
. ,

2001 L (CABI, 2007;

Robinson et al
. , 2001) Yes No

28 An internal feeder of fruit and stems (CABI, 2007) 29 This species bores into fruit (Robinson et al
. , 2001). 30 This species sometimes enters the fruit from the flower/calyx end, making it very difficult to be noticed (Nazeer Ahmad, 1942). Furthermore, it pupates in the fruit (Nazeer Ahmad, 1942).Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 22 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Lepidoptera**  **: Tortricidae**  **Archips micaceana** Walker IN(Manjunatha et al
. , 2003) Robinson et al
. ,

2001 L (Manjunatha et

al
. , 2003; Robinson et al
. , 2001) Yes No **Cydia pomonella** Li nnaeus IN , U.S. (CABI, 2007) Robinson et al
. ,

2007 N/A No N/A **Thysanoptera :**  **Thripidae Megalurothrips distalis**  (Karny) IN(CABI, 2007; Robinson et al
. , 2001) CABI, 2007 L , I , F (CABI, 2007) Yes No 31 **Retithrips syriacus** (Mayet) IN(Hill, 1983; Sheikh, 2006); U.S. (FL) (CABI, 2007) Hill, 1983 L (Hill, 1987; Sheikh, 2006) [Yes] 5 No **Rhipiphorothrips cruentatus**  (grapevine thrips) IN(Sheikh, 2006) CABI, 2007;

Sheikh, 2006 L (Sheikh, 2006);

L , F (CABI, 2007) Yes Yes **Scirtothrips oligochaetus** Kary ny Sy n: Anaphothrips oligochaetus Kary

ny IN(Karny, 1926; Sheikh, 2006) Sheikh, 2006 L (Palmer et al
. , 1990); I (Sheikh, 2006); F (Nair, 1975) Yes Yes **Thrips subnudula** Karny Sy n : Ramaswamiahiella subnudula (Mound, 2007) IN(Karny, 1926; Sheikh, 2006) Sheikh, 2006 L (Morton, 1987); I (Sheikh, 2006) Yes No
**WEEDS Loranthaceae**  **Loranthus spp.**  IN(Kumar, 1944) Kumar, 1944 St (Mabberley , 1987) Yes No **PATHOGENS Alternaria alternate**  (Fr.) Kiesl. (Dothideomycetes, Pleosporales) IN(Farr et al
. , 2007); U.S. (CABI, 2007) Farr et al
. , 2007 N/A No N/A

31 Although CABI (2007) lists M. distalis as a “minor” host of pomegranate, we found no other reference to substantiate this. Megalurothrips distalis appears to be mainly a pest of legumes, and only occasionally attacks fruit trees such as pome and stone fruit trees. Furthermore, M. distalis generally feeds on the flowers and leaves. These factors led us to conclude that this pest is unlikely to be in the pathway.Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 23 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Aspergillus flavus**  Li nk Teleomorph: Emericella ( Eurotiomycetes, Eurotiales) IN(Farr et al
. , 2007); U.S. (CABI, 2007) Farr et al
. , 2007 N/A No N/A **Aspergillus nidulans** (Eidam) G. Winter Teleomorph: Emericella ( Eurotiomycetes, Eurotiales) IN(Farr et al
. , 2007); U.S. (Farr et al
. , 2007) Farr et al
. , 2007 N/A No N/A
**Aspergillus niger** Tiegh Teleomroph: Emericella ( Eurotiomycetes, Eurotiales) IN(Farr et al
. , 2007); U.S. (CABI, 2007) Farr et al
. , 2007 N/A No N/A **Aspergillus niveus** Blochwitz Teleomorph: Emericella ( Eurotiomycetes, Eurotiales) IN( Sheikh, 2006); U.S. (Schering, 1990 ) Sheikh, 2006 N/A No N/A
**Aspergillus versicolor** (Vuill.) Tirabosch Teleomorph: Emericella ( Eurotiomycetes , Eurotiales) IN( Sheikh, 2006 ), U.S. (Farr et al
. , 2007) Sheikh, 2006 N/A No N/A

**Beltraniella humicola** P. Rama Rao Anamorph: Leiosphaerella (Sordariomycetes, Xylariales) IN(Farr et al
. , 2007) Farr et al
. , 2007 L (Farr et al
. , 2007) Yes No
**Botryodiplodia theobromae**  Pat (Incertae sedis) Lasiodiplodia theobromae (Pat.) Griffon & Maubl. Fungi Imperfecti: Coelomycetes IN(Farr et al
. , 2007); U.S. (CABI, 2007) Farr et al
. , 2007 N/A No N/APest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 24 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Botryotinia fuckeliina**  (de Bary) Whetzel Sy n : Ascom yc etes: Helotiales Anamorph: Botrytis fuckeliana cinerea Pers.: Fr. (Leotiomycetes, Helotiales) IN; U.S. (CABI, 2007) CABI, 2007 N/A No N/A

**Ceratocystis fimbriata** Ellis & Halst. Ceratocy stidaceae (Sardariomyc etes, Microascales) Anamorph: Chalara sp. IN(Farr et al
. , 2007); U.S. (CABI, 2007) Farr et al
. , 2007 St , F , L , wood (Harrington, 2004 #11) Yes 32 No 33

**Cercospora granati** Rawla Sy n: Pseudocercosporella granati (Rawla) Teleomorph; Mycosphaerella (Dothideomycetes, Capnodiales) IN(Farr et al
. , 2007) Farr et al
. , 2007 L (Rawla, 1971) Yes No

32 Ceratocystis fimbriata cause s a highly destructive wilt on pomegranate (Somasehara, 1999). The taxonomy of C
. fimbriata is very complex (Harrington and Baker, 2004). Its host range is very broad and recent research indicates that C. fimbria ta may contain more than one species (Baker et al
. , 2003). We found no indication of which genotype(s) of C. fimbriata causes pomegranate wilt. Ceratocystis fimbriata is already present in the United S tates, where it infects Populus, Platanus , and Ipomea sp (Harrington and Baker, 2004). However, molecular evidence indicates that the North American clade of C. fimbrata is genetically distinct from the clades in Latin America and Asia ( Baker et al
. , 2003; Harrington and Baker, 2004; Johnson et al
. , 2005 ). In addition, pomegranate is cultivated in the same county and even same field as infected almonds in California (Harrington, 2004) and the pomegranate has not become infected ( Edwards and Davis, 1988 ). Th at may mean that C. fimbriata in North America diff er s from the other clades. Thus, an infective strain of C. fimbriata could still be introduced from abroad . Based on similar reasoning, a U . S . Forest Service risk analysis of Eucalyp tus wood chips and untreated logs im

ported from South America rated C. fim briata as H igh risk , and requiring some form of management or risk mediation (Kleijunus et al
. , 2001). 33 We found no evidence that C. fimbriata is present in pomegranate fruit. C. fimbriata is typically a pathogen of wood or fiberous fruit, such as cacao (Harrington, 2004). Additionally, C. fimbriata causes a wilt of the bush and there has has been no description of a fruit rot (Huang et al, 2003). Based on the lack of observations of fruit rot and the description of the disease as a wilt rather than a fruit rot, it seems that fruit is not infected by the fungus and makes a poor pathway . If additional observations contradict this, a reanalysis can be done.Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 25 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Cercospora punicae**  Henn . Sy n: Pseudocercospora punicae (Henn.) Deighton Teleomorph: Mycosphaerella lythracearum F. A. Wolf (Dothideomycetes, Capnodiales) IN(Farr et al
. , 2007); U.S. (Farr et al
. , 2007) Farr et al
. , 2007 N/A No N/A

**Cladosporium oxysporum** Berk. & M. A. Curtis Fungi Imperfecti: Hy phom yc etes IN( Sheikh,

2006 ); U.S. (Farr et al
. , 2007) Sheikh, 2006 N/A No N/A
**Cochliobolus lunatus** R.R. Nelson & Haasis Ascomycetes: Dothideales Anamorph: Pleosporaceae Curvularia lunata (Wakk.) Boedijn (Dothideomycetes, Pleosporales) IN(CABI, 2007); U.S. (Farr et al
. , 2007) Farr et al
. , 2007 N/A No N/A

**Coleophoma empetri** (Rostr.) Petr. Fungi Imperfecti: Coelomycetes IN( Sheikh, 2006 ); U.S. (Farr et al
. , 2007) Sheikh, 2006 N/A No N/A
**Colletotrichum gloeosporioides**  (Penz.) Penz. & Sacc. in Penz. Fungi Imperfecti: Coelomycetes IN( Sheikh, 2006 ); U.S. (Farr et al
. , 2007) Sheikh, 2006 N/A No N/A
**Coniella granati** (Sacc.) Petr. & S yd. Anamorph: Schizoparme (Sordariomycetes, Diaporthales) IN(CABI, 2007); U.S. (Farr et al
. , 2007) Farr et al
. , 2007 N/A No 34 N/A
**Coniothyrium sp.**  Anamorph: Leptosphaeria (Dothideomycetes, Pleosporales) IN(Farr et al
. , 2007) Farr et al
. , 2007 L , St (Farr et al
. , 2007) Yes No

34 Coniella granati is described alt ernately as a saprophyte ( PestID , 2007) and as a pathogen ( Weber, 1973 ) . Consequently , we included it on the pest list, but its presence in the United S tates prevents its designation as a quarantine pest.Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 26 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Curvularia pallescens**  Boedijn (Dothideomycetes, Pleosporales) Teleomorph: Cochliobolus pallescens (Tsuda & Ueyama) Sivan. IN , U.S.(Farr et al
. , 2007) Farr et al
. , 2007 N/A No N/A

**Discosia punicae** Schreem . & M. Reddy (Sordariomycetes, Xylariales) IN(Farr et al
. , 2007) Farr et al
. , 2007 L (Madhukar and Reddy , 1988) Yes No **Fusarium equiseti** (Corda) Sacc. Fungi Imperfecti: Hy phom yc etes IN( Sheikh,

2006 ); U.S. (Farr et al
. , 2007) Sheikh, 2006 N/A No N/A **Fusarium fusarioides** (Gonz. Frag. & Cif.) C. Booth Teliomorph: Gibberalla (Sordariomycetes, Hy pocreales) IN(Farr et al
. ,

2007); U.S. (Farr et al
. , 2007) Farr et al
. , 2007 N/A No N/A

**Gloeosporium sp.**  (Leotiomycetes, Helotiales) IN(Farr et al
. , 2007) Farr et al
. , 2007 L (Farr et al
. , 2007) Yes No **Leptosphaeria punicae** A.K. Kar & Maity Teleomorph: (Dothideomycetes, Pleosporales) Leptosphaeriaceae Loculoascomyc etes: Dothideales IN(Farr et al
. , 2007) Farr et al
. , 2007 R , L Yes No

**Nigrospora oryzae** (Berk. & Broome) Petch (Sordariomycetes In certae sedis) IN(Farr et al
. , 2007); U.S. (Farr et al
. , 2007) Farr et al
. , 2007 N/A No N/A
**Oidium** sp. IN(Farr et al
. , 2007) Farr et al
. , 2007 L , F (Farr et al
. , 2007) Yes Yes **Penicillium chrysogenum** Thom Fungi Imperfecti: Hy phom yc etes IN( Sheikh,

2006 ), U.S. (Farr et al
. , 2007) Sheikh, 2006 N/A No N/APest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 27 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Penicillium expansum**  Link Teleomorph: Eupenicillium(Eurotiom yc etes, Eurotiales) IN(Farr et al
. , 2007), U.S. (CABI, 2007) Farr et al
. , 2007 N/A No N/A
**Penicillium sp**  **.** Teleomorph: Eupenicillium (Eurotiomycetes, Eurotiales) IN(Farr et al
. , 2007) Farr et al
. , 2007 F (Farr et al
. , 2007) Yes Yes
**Pestalotia jodhpurensis** Bilgrami & Purohit Teleomorph: Broomella (Sordariomycetes, Xylariales) IN(Farr et al
. , 2007) Farr et al
. , 2007 L (Misra, 2004) Yes No
**Pestalotiopsis versicolor** ( Speg.) Steyaert Teleomorph: Pestalosphaeria (Sordariomycetes, Xylariales) IN(Farr et al
. , 2007) Farr et al
. , 2007 L (Madhukar and Reddy , 1988) Yes No

**Phaeoramularia legrellei** V.G. Rao & B.R.D. Yadav (Dothideomycetes, Capnodiales) IN(Farr et al
. , 2007) Farr et al
. , 2007 L (Rao and Yadav, 1991) Yes No

**Phoma** sp. IN(Farr et al
. , 2007) Farr et al
. , 2007 N/A Yes Yes **Phomopsis aucubicola** (Brunaud) Grove Sy n : Phoma lirelliformis Sacc. var. aucubicola Brunaud Teleomorph: Diaporthe (Sordariomycetes, Diaporthales) IN(Farr et al
. , 2007), U.S.( Ny vall and Hu, 1997) Farr et al
. , 2007 N/A No N/A

**Phomopsis** sp**.** IN(Farr et al
. , 2007) Farr et al
. , 2007 Twigs (Farr et al
. , 2007) Yes No **Phomopsis versoniana** Sacc. Teleomorph: Diaporthe (Sordariomycetes, Diaporthales) IN(Farr et al
. , 2007); U.S. (Dodge, 1936) Farr et al
. , 2007 N/A No N/APest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 28 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Phyllosticta sp.**  IN(Farr et al
. , 2007) Farr et al
. , 2007 L (Farr et al
. , 2007) Yes No **Rhizopus arrhizus** A. Fisch A. Fischer Sy n : Rhizopus oryzae Went & Prins. Geerl. Prinsen Geerligs (Zygom yc etes, Mucorales ) IN(Farr et al
. , 2007); U.S. (Farr et al
. , 2007) Farr et al
. , 2007 N/A No N/A

**Rhizopus stolonifer** (Ehrenb.) Vuill. (Ehrenb.: Fr.) Vuill. (Zygom yc etes, Mucorales) IN(Farr et al
. , 2007); U.S. (Farr et al
. , 2007) Farr et al
. , 2007 N/A No N/A
**Sclerotium rolfsii** Sacc. Sy n : Teleomorph: Athelia rolfsii (Curzi) C.C. Tu & Kimbrough (Agaricomyc etes, Agaricales) IN(Farr et al
. , 2007); U.S. (Farr et al
. , 2007) Farr et al
. , 2007 N/A No N/A

**Setosphaeria rostrata** K. J. Leonard (Dothideomycetes, Pleosporales) IN(Bose et al
. , 2002); U.S. (Farr et al
. , 2007) Farr et al
. , 2007 N/A No N/A **Sphaceloma punicae** Bitanc. & JenkinsTeleomorph Elsinöe (Dothideomycetes, My riangiales) IN(Farr et al
. ,

2007); U.S. (Farr et al
. , 2007) Farr et al
. , 2007 N/A No N/A

**Sphaeropsis punicae** Unknown taxonomy IN(Farr et al
. , 2007) Farr et al
. , 2007 L (Mathur, 1979) Yes No **Spicaria sp**  . Unknown taxonomy IN(Farr et al
. , 2007) Farr et al
. , 2007 N/A Yes Yes **Syncephalastrum racemosum**  Cohn ex. J. Schrot. Zygom yc etes: Mucorales IN( Sheikh, 2006 ); U.S. (Farr et al
. , 2007) Sheikh, 2006 N/A No N/APest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 29 **Pest Distribution**  **Host Association Plant p**  **arts affected**  **1 Q**  ****

**Status Follows**  **p athway** **2 Chromistidae**  **Phytophthora nicotianae** Breda de Haan (Oomycetes, Peronosporales) IN(Farr et al
. , 2007); U.S. (Farr et al
. , 2007) Farr et al
. , 2007 N/A No N/A **Phytophthora palmivora var.**  **palmivora** (E .J. Butler) E.J. Butler (Oomycetes, Peronosporales) IN( Farr et al
. , 2007); U.S. (Farr et al
. , 2007) Farr et al
. , 2007 N/A No N/A

**BACTERIA Xanthomonas axonopodis**  **pv. Punicae**  (Hingorani & Singh 1959) Xanthomonads IN(Upasana and Verma, 2002) Upasana and

Verma, 2002 L , F , St (Upasana

and Verma, 2002) Yes Yes
**NEMATODES Heliocotylenchus**  **pseudorobustus** IN(CABI, 2007) CABI, 2007 R (CABI, 2007) Yes No **Heliocotylenchus spp**  **.** (Steiner) Golden ( Hoplolaiminae ) ( Hoplolaiminae ) IN(Darekar et al
. , 1989) Darekar et al
. ,

1989 R (Darekar et al
. ,

1989) Yes No **Meloidogyne incognita** Kofoid & White ( Heteroderidae) IN(Darekar et al
. , 1989); U.S. (Maggenti, 1981) Darekar et al
. ,

1989 R (Darekar et al
. ,

1989) No No **Xiphinema insigne** Lo os ( Lo ngidoridae) IN(Darekar et al
. , 1989) Darekar et al
. ,

1989 R (Darekar et al
. ,

1989) Yes No **VIROIDS Hop Stunt Viroid**  Pospiviroidae IN(Roy et al
. , 2005); U.S. (Sano et al
. , 1986) Astruc et al
. , 1996 N/A No N/A
**VIRUSES Cucumber mosaic virus**  Bromoviridae IN(Verma et al
. , 2004); U.S. (CABI, 2007) Verma et al
. , 2004 N/A No N/A

**2.5 .**  **Quarantine Pests Likely to Follow the Pathway**  Most of the pests identified in Table 4 are found in the conterminous United States, so are not quarantine pests . Furthermore, not all of the quarantine pests identified are likely to follow thePest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 30 pathway of fresh pomegranate fruit. Quarantine pests not expected to follow the pathway were not considered for further anal ys is . S hould any of these pests be intercepted in shipments of the commodity , however, quarantine action may be taken and additional risk analy ses may be done . The quarantine pes ts that were considered likely to follow the pathway

are listed below (Table 5). The armored scale, Pseudaonidia trilobitiformis (Hemiptera: Diaspididae) was not anal yz ed because although armored scales may enter on commercial fruit for consumption, they are not expected to establish via this pathway (Miller, 1985; Zlotina et al
. , 2007). In a recent review, Zlotina and Hennessey

(2 007) concluded that even if high quantities of imported fruit are infested with armored scale species, there is a very low risk that an armored scale would establish along a commercial fruit pathway 35
. This low risk is explained by the poor ability

of armored scales to disperse to new host plants from fruits for consumption (Zlotina et al
. , 2007). The following characteristics of armored scales contribute to their poor dispersal capabilities:  Legs and wings are absent in females and in feeding immature form s ; only short
- lived m ales possess wings, and they do not feed and tend to mate with nearby females.  Self
- dispersal of armored scales occurs b y immature forms, or “crawlers”. They are the most vulnerable life stage, survival of which decreases with long distance wind dispersal. Crawlers passivel y disperse b y wind from one plant to another for onl y about 24 hours. After crawlers start feeding, they do not disperse further because they soon lose their legs and are anchored firml y t o the host by their mouthp arts.  Dispersal from fruit discarded in the environment is considered very unlikely because of low wind speeds at ground level , and low survival rate of crawlers , either on the ground , on decaying fruit , or on fruit peel s . Crawlers are highl y unlikely to w alk away from their natal host because they cannot move rapidly over bare soil or rough surfaces. **Table 5** . Quarantine pests likely to follow the pathway

on fresh pomegranate f

ruit from India. **Group: Family Pest name(s)**  Acari: Eriophyi dae Tenuipalpus granati, T. punicae Diptera: Tephritidae Bactrocera carambolae, B. dorsalis, B. zonata Hemiptera: Coccidae Ceroplastes rubens, C. rusci Hemiptera: Margarodidae Drosicha dalbergiae, Icerya aegyptiaca, I. seychellarum Hemiptera: Pseudococcidae Dysmicoccus neobrevipes, Maconellicoccus hirsutus, Nipaecoccus viridis, Planococcus angkorensis, P. lilacinus, Pseudococcus cryptus Lepidoptera: Crambidae Conogethes punctiferalis Lepidoptera: Lycaenidae Deudorix epijarbas, D. isocrates Lepidoptera: P yralidae Cryptoblabes gnidiella, Dichocrocis megillalis, Euzophera bigella Thy sanoptera: Thripidae Rhipiphorothrips cruentatus, Scirtothrips oligochaetus Bacteria Xanthomonas axonopodis pv. punicae

35 Their conclusions apply to commercial fruit shipp ed without leaves, stems, or contaminants.Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 31 **2.6 .**  **Consequences of Introduction** The undesirable consequences that may occur from the introduction of quarantine pests are assessed in this section. For each quarantine pest, the potential consequences of introduction were rated in five areas called “Risk Elements.” The Risk Elements include: Climate/Host Interaction, Host Range, Dispersal Potential, Economic Impact and Environmental Impact. These Risk Elements reflect the biology , host range and climatic/geographic distribution of each pest and are supported b y biological information on e ach of the analyzed pests summarized in this section. For each risk element, pests were assigned a rating of Low (1 point), Medium (2 points) or High (3 points). The ratings were determined using the criteria in the Risk Assessment Guidelines, Version 5.02 (USDA, 2000). A cumulative risk value was then calculated by summing the ratings. The following scale was used to interpret this total: 5
- 8 points = low risk, 9 - 12 points = medium risk, and 13
- 15 points = high risk. The ratings are summarized in Table 6 . The major sources of uncertainty present in this risk assessment are similar to those in other risk assessments. They

include the use of a developing or evolving process (Orr et al
. , 1993; USDA, 2000), the approach used to combine risk elements (Bier, 19 99; Morgan and Henrion, 1990), and the evaluation of risk b y comparisons to lists of factors within the guidelines (Kaplan, 1992; Orr et al
. , 1993). To address this last source of uncertainty , the lists of factors were interpreted as illustrative and not e xhaustive. This implies that additional biological information, even if not explicitly part of the criteria, can be used when it is relevant to a rating. Other sources of uncertainty are the quality of the biological information and the amount of informati on available on the regional flora and fauna. Inherent biological variation within a population of organisms introduces uncertainty

as well (Morgan and Henrion, 1990).
**Bactrocera carambolae (Hendel) (Diptera: Tephritidae)**  **Risk r**  **ating s**  **Risk Element #1: Risk Element #1: Climate/Host Interaction** Bactrocera carambolae is found in Brunei Darussalam, tropical India, Indonesia, Malay sia, Singapore, and Thailand . It also ha s restricted distributions in Brazil, French Guiana, and Suriname . It was apparentl y eradicated from Guyana (CABI, 2007). Based on this distribution we estimate that B. carambolae could only become established in the areas of the United States corresponding to Plant Hardiness Zone 10 (USDA, 1990). One or more of its potential hosts occurs in this zone ( NRCS , 2007). Low (1)

**Risk Element #2: Host Range** This pest attack s many species in multiple families including: **Anacardiaceae** ( Anacardium occidentale, Mangifera spp. , Spondias purpurea ), **Annonaceae** ( Annona spp., Rollinia pulchrinervis ), **Apocynaceae** ( Thevetia peruviana ), **Arecaceae** ( Arenga spp.), **Caricaceae** ( Carica papaya ), **Clusiaceae** ( Garcinia mangostana ), **Combretaceae** ( Terminalia catappa ), **Lauraceae** (Persea americana ), **Moraceae** ( Artocarpus spp), **Myrtaceae** (

Eugenia uniflora, Psidium spp. , Syzygium spp.), **Oxalidaceae** ( Averrhoa spp.), **Punicaceae** (

Punica granatum ), **Rhamnaceae** (Ziziphus spp.), **Rhizophoraceae** ( Rhizophora spp.), **Rutaceae** ( Citrofortunella mitis, Citrus spp, Fortunella margarita ), **Sapotaceae** ( Chrysophyllum cainito, Mimusops elengi, Pouteria campechiana ), High (3)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 32 **Bactrocera carambolae (Hendel) (Diptera: Tephritidae)**  **Risk r**  **ating s**  **Solanaceae** ( Capsicum annuum, Solanum lycopersicum ) (CABI, 2007). **Risk Element #3: Dispersal Potential** Tephritid fruit flies have a relativel y high biotic potential. For example, in laboratory studies, the f

ecundity of female B. tryoni and B. dorsalis fruit flies was over 1000 eggs (Fletcher, 1989a). Like other Bactrocera, B. carambolae disperses readily by flight (Hely et al
. , 1982); many Bactrocera species can fl y 50 - 100 km (Fletcher, 1989b). Bactrocera f ruit flies can also disperse via infested plant materials, such as fruits and flowers (Fletcher, 1989b; CABI, 2007). For example, since 1985, species of Bactrocera have been intercepted at U.S. ports of entry over 12,000 times, in a variety of commodities ( PestID , 2007). High (3)

**Risk Element #4: Economic Impact** B. carambolae attacks several economicall y important crops , including avocado, tomato, pepper and Citrus spp. , and would likely be a major pest on these crops in the United States (Drew et al
. , 1982; White and Elson
- Harris, 1992). Up to 100 percent of unprotected crops may

be damaged (CABI, 2007). The introduction of B. carambolae in the continental United States would likely stimulate eradication or control programs, similar to those that have been implemented in the past for other Tephritid fruit flies (CABI, 2007; Clausen, 1978). Additionally , its presence in the United States, even as a temporary adventive population, could lead to severe export restrictions of host commodities to markets out side of this pest’s known distribution (Drew et al
. , 1982). High (3)

**Risk Element #5: Environmental Impact** B. carambolae may

have direct impacts on Federal listed endangered species such as Ziziphus celata (in Florida), which is closel y related to other hosts known to be attacked by B. carambolae (USFWS, 2007). Because it represents a potential threat to the Citrus and other crop industries, the establishment of B. dorsalis in the United States would likely initiat e eradication or chemical/biological control programs, similar to those that have occurred in Hawaii and California, and Florida for other Tephritid fruit flies (CABI, 2007; Clausen, 1978). High (3)

**Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)**  **Risk r**  **ating s**  **Risk Element #1: Climate/Host Interaction** Bactrocera dorsalis is found in Bhutan, southern China, northern India, Myanmar, northern Thailand, Guam, and the Hawaiian Islands (White and Elson
- Harris, 1992). Based on this distribution we estimate that B. dor salis could become established in the areas of the United States corresponding to Plant Hardiness Zone 9 - 10 (USDA, 1990). One or more of its potential hosts occurs in this zone ( NRCS , 2007). Medium (2)

**Risk Element #2: Host Range** B. dorsalis attack s multiple species in multiple families including the following : **Anacardiaceae** (

Anacardium occidentale, Mangifera foetida, Spondias purpurea, High (3)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 33 **Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)**  **Risk r**  **ating s**  Mangifera indica ), **Annonaceae** ( Annona spp.), **Arecaceae** ( Areca catechu ), **Caricaceae** ( Carica papaya ), **Combretaceae** ( T erminalia catappa ), **Cucurbitaceae** ( Cucumis melo, Cucumis sativus, Momordica charantia ), **Ebenaceae** ( Diospyros kaki), **Flacourtiaceae** (

Flacourtia indica ), **Lauraceae** (Persea americana ), **Malpighiaceae** ( Malpighia glabra ), **Moraceae** ( Artocarpus spp. , Ficus racemos a ), **Musaceae** ( Musa ), **Myrtaceae** (Syzygium spp. , Psidium guajava ), **Oxalidaceae** ( Averrhoa carambola ), **Punicaceae** (

Punica granatum ), **Rhamnaceae** (Ziziphus spp.), **Rosaceae** (Prunus spp., Malus pumila, Pyrus communis ), **Rubiaceae** ( Coffea Arabica ), **Rutaceae** ( Aegle m armelos, Citrus spp.), **Sapindaceae** ( Dimocarpus longan , Nephelium lappaceum, Litchi chinensis ), **Sapotaceae** ( Chrysophyllum cainito, Mimusops elengi, Manilkara zapota ), **Solanaceae** ( Capsicum annuum ), and **Tiliaceae** ( Muntingia calabura ) (CABI, 2007; White and Elson
- Harris, 1992). **Risk Element #3: Dispersal Potential** Tephritid fruit flies have a relativel y high biotic potential. Depending on climate, B. dorsalis may

have five or more overlapping generations per year (Shi et al
. , 2005). In laboratory

studies, the fecundity

of female B. tryoni and B. dorsalis fruit flies was estimated to be over 1000 eggs (Fletcher, 1989a). Many Bactrocera species can fl y 50 - 100 km (Fletcher, 1989b), and B. dorsalis also disperses readily

by flight (Hely et al
. , 1982) . Additionally, Bactrocera can be dispersed by infested plant materials, such as fruits and flowers (Fletcher, 1989b; CABI, 2007). For example, since 1985, Bactrocera species have been intercepted at U.S. ports of entry in a variety

of commodities over 12,00 0 times ( PestID , 2007). High (3)

**Risk Element #4: Economic Impact** Bactrocera dorsalis has been reported on several economicall y important crops including apple, peach, pear, strawberry , and Citrus spp. , and could be a major pest on these crops in the United States (Drew et al
. , 1982; White and Elson
- Harris, 1992). It may damage u

p to 100 percent of unprotected crops (CABI, 2007). The introduction of B. dorsalis in the continental United States would likely stimulate eradication or control programs, as has been done in the recent past for other Tephritid fruit flies (CABI, 2007; Clausen, 1978). Its presence in the United States, even as a temporary

adventive population, could lead to severe export restrictions of host commodities to markets outside of this pest’s known distribution (Drew et al
. , 1982). High (3)

**Risk Element #5: Environmental Impact** Bactrocera dorsalis may

directly affect Federal listed endangered species such as Prunus geniculata and Ziziphus celata (in Florida), which are closel y related to other hosts known to be attacked by B. dorsalis (USFWS, 2007). Because it threat ens Citrus, stone fruit, and other crop industries, the establishment of B. dorsalis in the United States could trigger the initiation of eradi cation or chemical and biological control programs, similar to those that have occurred in Hawaii, Florida and California (CABI, 2007; Clausen, 1978). High (3)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 34 **Bactrocera zonata (Hendel) (Diptera: Tephritidae)**  **Risk r**  **ating s**  **Risk Element #1: Climate/Host Interaction** Bactrocera zonata is found in India, Indonesia (Sumatra), Laos, Sri Lanka, Thailand, and Vietnam (White and Elson
- Harris, 1992). Based on this distribution we estimate that B. zonata could become established in United States Plant Hardiness Zone s 9 - 10 (USDA, 1990). One or more of its potential hosts occurs in this zone ( NRCS , 2007). Medium

(2) **Risk Element #2: Host Range** B actrocera zonata attack s multiple species in multiple families including: **Annonaceae** ( Annona spp.), **Arecaceae** ( Phoenix dactylifera ), **Caricaceae** ( Carica papaya ), **Combretaceae** ( Terminalia catappa ), **Cucurbitaceae** ( Cucumis melo, Citrullus lanatus, Momordica spp.), **Lythraceae** (Punica granatum ), **Malvaceae** ( Abelmoschus esculentus, Grewia asiatica ), **Moraceae** ( Ficus spp.), **Myrtaceae** (Syzygium spp. , Psidium guajava ), **Rosaceae** ( Cydonia oblonga , Malus spp., Prunus spp.), **Rhamnaceae** (Ziziphus spp.), **Rutaceae** ( Aegle marmelos, Citrus spp.), **Solanaceae** (Solanum melongena, Solanum lycopersicum ) (CABI, 2007; White and Elson
- Harris, 1992). High (3)

**Risk Element #3: Dispersal Potential** The reproductive potential, active dispersal potential, and the potential for being dispersed on infested plant materials for B. zonata is no different from B. dorsalis above. High (3) **Risk Element #4: Economic Impact** B actrocera zonata has been reported on several economically important crops including stonefruit, Citrus spp. and Solanum spp. and could be a major pest on these crops in the United States (Drew et al
. , 1982; White and Elson
- Harris, 1992). The introduction of B. zonata in the continental United States would likely stimulate eradication or control programs, similar to those that have been implemented in the past for other Tephritid fruit flies (CABI, 2007; Clausen, 1978). Its presence in the United States, even as a temporary adventive population, could lead to severe export restrictions of host commodities to markets outside of this pest’s known distribution (Drew et al
. , 1982). High (3)

**Risk Element #5: Environmental Impact** This risk element is the same for B. zonata as it was for B. dorsalis above. High (3) **Ceroplastes rubens Maskell (Hemiptera: Coccidae)**  **Risk r**  **ating s**  **Risk Element #1: Climate/Host Interaction**  Ceroplastes rubens is distributed from warm temperate zones to the tropics. It is found in east and south Asia, Oceania (including Australia), eastern and southern parts of Africa, and sporadically

through the Caribbean (CABI , 2007 ; Dekle, 2001) . In the United States, it occurs in Florida and Hawaii. This corresponds to Plant Hardiness Zones 8
- 12 (Anony mous, 2007; Maxwell, 2007) , or Zones 8 - 10 in the continental United States. One or more of its potential hosts occurs in these zones in the United States ( NRCS , 2007). Medium (2)

**Risk Element #2: Host Range** Ceroplastes rubens is highly poly phagous, attacking numerous host families and genera, including: **Acanthaceae** (Strobilanthes ), **Aceraceae** ( Acer), **Am aranthaceae**  ( Celosia ), High (3)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 35 **Ceroplastes rubens Maskell (Hemiptera: Coccidae)**  **Risk r**  **ating s**  **Anacardiaceae** (

Anacardium , Mangifera , Rhus , Schinus ), **Annonaceae** ( Annona ), **Apocynaceae** (

Allamanda , Alyxia , Melodinus , Nerium , Plumeria , Thevetia ) **Aquifoliaceae** (

Ilex ), **Araceae** (

Aglaonema , Anthurium , Dieffenbachia , Epipremnum , Philodendron , Syngonium , Monstera , Zantedeschia ), **Araliaceae** ( Aralia , Brassaia , Dizygotheca , Fatsia , Hedera , Meryta , Polyscias , Schefflera ), **Arecaceae** ( Cocos ), **Aspleniaceae** (

Asplenium ), **Asteraceae** ( Artemisia , Chrysanthemum , Fitchia , Gerbera , Helianthus ), **Barringtoniaceae** ( Barringtonia ), **Berberidaceae** ( Nandina ), **Bischofiaceae** (

Bischofia ), **Bixaceae** (

Bixa ), **Blechnaceae** (

Blechnum ), **Buxaceae** ( Buxus ), **Campanulaceae** (Siphonodon ), **Caprifoliaceae** ( Viburnum ), **Celastraceae** ( Celastrus , Elaeodendron , Euonymus ), **Cephalotaxaceae** ( Cephalotaxus ), **Clusi aceae**  ( Calophyllum , Garcinia , Montrouziera ), **Cunoniaceae** ( Weinmannia ), **Cycadaceae** ( Cycas ), **Davalliaceae** (

Arthropteris , Davallia ), **Dicksoniaceae** ( Cibotium ), **Ebenaceae** ( Diospyros ), **Elaeocarpaceae** (Elaeocarpus ), **Ericaceae** (Rhododendron ), **Euphorbiaceae** (

Euphorbia ), **Fabaceae** (

Acacia , Cytisus , Dioclea , Inocarpus , Palaquium , Spartium ), **Gleicheniaceae** ( Dicranopteris , Gleichenia ), **Heliconiaceae** ( Heliconia ), **Hernandiaceae** ( Hernandia ), **Hypoxidaceae** ( Molineria ), **Lauraceae** ( Cinnamomum , Cryptocarya , Laurus , Lindera , Machilus , Persea ), **Lomariopsidaceae** ( Elaphoglossum ), **Loranthaceae** ( Loranthus ), **Magnoliaceae** ( Illicium , Magnolia ), **Malvaceae** (

Hibiscus ), **Melastomataceae** ( Astronidium ), **Monimiaceae** ( Wilkiea ), **Moraceae** (

Artocarpus , Cudrania , Ficus , Morus ), **Musaceae** ( Musa ), **Myristicaceae** ( Myristica ), **Myrsinaceae** ( Ardisia , Myrsine , Rapanea ), **Myrtaceae** ( Agonis , Callistemon , Eucalyptus , Eugenia , Feijoa , Melaleuca , Metrosideros , Pimenta , Psidium , Rhodomyrtus , Syzygium ), **Nyctaginaceae** ( Bougainvillea ), **Oleaceae** ( Ligustrum , Olea ), **Oleandraceae,** Nephrolepis ), **Orchidaceae** ( Grammatophyllum , Stanhopea ), **Pinaceae** ( Pinus ), **Piperaceae** (

Peperomia Macropiper , Piper), **Pittosporaceae** ( Pittosporum ), **Poaceae** ( Tristania ), **Polygonaceae** ( Coccoloba ), **Polypodiaceae** ( Belvisia , Phymatodes , Platycerium , Polypodium ), **Pteridaceae** ( Acrostichum ), **Punicaceae** ( Punica ), **Rhizophoraceae** ( Rhizophora ), **Rosaceae** ( Chaenomeles , Eriobotrya , Malus , Photinia , Prunus , Pyrus , Spiraea ), **Rubiaceae** ( Coffea , Gardenia , Gouldia , Ixora , Paederia , Psychotria , Randia , Straussia ), **Rutaceae** ( Citrus , Eremocitrus , Evodia , Flindersia , Pelea , Poncirus ), **Santalaceae** ( Exocarpos ), **Sapindaceae** ( Cupaniopsis , Dimocarpus , Euphoria , Litchi , Nephelium ), **Sapotaceae** ( Calocarpum , Pouteria ), **Schisandraceae** (

Kadsura ), **Sinopteridaceae** ( Aspidotis , Pellaea ), **Stilaginaceae** ( Antidesma ), **Symplocaceae** (Symplocos ), **Tamaricaceae** ( Tamarix), **Taxaceae** ( Podocarpus ), **Theaceae** ( Camellia , Cleyera , Eurya , Ternstroemia ), **Thymelaeaceae** ( Daphne), **Ulmaceae** ( Celtis ), **Verbenaceae** ( Premna ), **Zingiberaceae** ( Alpinia , Zingiber) (Ben
- Dov et al
. , 2007). **Risk Element #3: Dispersal Potential** With a mean fecundity

of approximately

300 eggs per female and two generations per y ear (CABI , 2007), C. rubens has high reproductive potential. InAustralian studies approximately

50 percent of the hatchings were lost (CABI, 2003). S oft scales (Coccoidea) in general (Greathead, 1997) and C. rubens in particular (I tioka and I

noue, 1991; Loch and Zalucki, 1996), have a limited ability for natural dispers al , their potential may neverthe less be high due to transport of infested plant material (CABI , 2007). This is indicated b y the fact that it has been introduced to many different parts of the world High (3)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 36 **Ceroplastes rubens Maskell (Hemiptera: Coccidae)**  **Risk r**  **ating s**  outside its native Africa and India (CABI, 2007; EPPO, 2004). Since 1985, C. rubens has be en intercepted at U.S. ports
- of
- entry over 4,700 times on plants and other plant materials (PestID , 2007). **Risk Element #4: Economic Impact** Ceroplastes rubens is a pest of citrus, coffee, tea, cinnamon, mango, avocado, and litchi. I t damages its hosts by

piercing and sucking plant tissues (leaves, shoots, and fruit) (CABI , 2007). Under severe infestations, leaves and fruit will prematurely drop resulting in y

ield loss (van den Berg et al
. , 2001). Ceroplastes rubens also indirectly damages plant tissues by produc ing honey

dew , which promotes the growth of sooty mold. Sooty

mold reduces photosy nthetic rates and reduces the market value of fruit (van den Berg et al
. , 2001). Ceroplastes rubens has a limited distribution in Flo rida but is not currentl y under any apparent official control; further introductions and spread are unlikel y to result in the loss of additional foreign markets for domestically produced commodities. Medium (2)

**Risk Element #5: Environmental Impact** It is unknown if establishment of C. rubens in natural areas would reduce community biodiversity

or disrupt ecological processes; at least one other scale insect has shown the potential to be particularly invasive and damaging in natural areas (Howard et al
. , 2004). Because of its broad host range, C. rubens is likely to harm numerous federally Threatened and Endangered (T&E) species in the United States, particularly congeners of current hosts. Additional T& E species it may impact, beyond those already present in Florida, include Asplenium scolopendrium var. americanum (AL, M I, NY, TN), Helianthus paradoxus (NM, TX), H. schweinitzii (NC, SC), Rhus michauxii (GA, NC, SC, VA), Spiraea virginiana (GA, KY, NC, OH , PA, TN, VA, WV) (USFWS, 2007). Because C. ruben s is a major pest of citrus (van den Berg et al
. , 2001) , its wider establishment in the United States, particularl y California, may result in the initiation of chemical and/or biological control programs as it has in other places where this pest has invaded (CABI , 2007). High (3)

**Ceroplastes rusci Maskell (Hemiptera: Coccidae)**  **Risk r**  **ating s**  **Risk Element #1: Climate/Host Interaction** Ceroplastes rusci is present in many countries of Africa, the Middle East, southern Europe, and the Caribbean; parts of Asia (Indonesia, India); and parts of South America (Argentina, Brazil, Guyana, and Uruguay) (CABI, 2007). In 1994, it was discovered in Florida; it is currentl y present in Broward, Dade, Manatee, Palm Beach, Pa sco, and Pinellas counties (Hamon and Mason, 2001). Based on its distribution, we estimate it could survive in Plant Hardiness Zones 8
- 10 in the continental United States (USDA, 1990). One or more of its potential hosts occurs in these Zones (NRCS , 200 7 ). Medium (2)

**Risk Element #2: Host Range** Ceroplastes rusci is highly pol yphagous, with host plants belonging to 45 genera in 42 different plant families (CABI, 2007) including **Annonaceae** ( Annona , Annona cherimola , Annona muricata , Annona reticulata , Annona squamosa ), **Rutaceae** ( Citrus , Citrus aurantium , Citrus limon , Citrus x paradise ), **Moraceae** ( Ficus carica, Ficus, Morus ), **Actinidiaceae** (

Actinidia chinensis ), **Rosaceae** ( Crataegus , Cydonia , Mespilus High (3)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 37 **Ceroplastes rusci Maskell (Hemiptera: Coccidae)**  **Risk r**  **ating s**  germanica , Prunus , Pyrus communis ), **Euphorbiaceae** (Euphorbia longana ), **Malvaceae** ( Gossypium ), **Araliaceae** ( Hedera helix), **Aquifoliaceae** ( Ilex aquifolium ), **Convolvulaceae** (

Ipomoea batatas ), **Juncaceae** ( Juncus ), **Sapindaceae** (

Litchi chinensis , Nephelium lappaceum ), **Anacardiaceae** ( Mangifera indica , Pistacia ), **Mu saceae**  (

Musa ), **Myrtaceae** (

Myrtus communis , Psidium guajava ), **Lauraceae** ( Persea americana ), **Piperaceae** ( Piper nigrum ), **Platanaceae** ( Platanus ), **Salicaceae** ( Populus ), **Liliaceae** (

Ruscus ), **Vitaceae** ( Vitis vinifera ), and **Apocynaceae** ( Nerium oleander) (CABI, 2007). **Risk Element #3: Dispersal Potential** The biotic potential of C
. rusci is relatively high, with one or two generations per year, depending on the climate (Ben
- Dov, 1993; CABI, 2007). Females usually lay between 800 and 1500 eggs (CABI, 200 7). S oft scales (Coccoidea) in general (Greathead, 1990), and C. rusci in particular, have limited natural mobility (Hamon and Mason, 2001; Vu et al
. , 2006) . Adult males can fly , but females can only crawl to suitable feeding sites as first instar nymphs (“crawlers”) (CABI, 2007). Dispersal of the crawlers can be aided by wind and other animals (CABI, 2007; Gullan and Kosztarab, 1997). Scale insects, in general, can be transferred b y wind a distance of a few meters up to several km from the original plant (Gullan and Kosztarab, 1997). High (3)

**Risk Element #4: Economic Impact** Attack s by C. rusci weaken and kill leaves and shoots; its excreted honeydew provides a medium for the development of a sooty fungal film, which impairs photosy nthesis. Ceroplastes rusci frequently injures crops and ornamental plants, particularly when introduced into new areas lacking natural enemies (CABI, 2007). It is a pest of cultivated citrus and figs (Hamon and Mason, 2001) , and while it is usually only a minor pest of citrus, it can sometimes be serious (Ben
- Dov, 1993). Infrequent major local outbreaks in citrus have been reported in Ital y and Greece, but have been controlled by the application of oils (Hamon and Mason, 2001). In addition, C. rusci attacks other economicall y important U.S. crops, such as cotton, sweet potato, banana, Prunus species, and grapes (CABI, 2007). In Florida , it is a pest of ornamental plants in the genus Ixora , but is found infrequentl y on other host plants (Hamon and Mason, 2001). Ceroplastes rusci has a limited distribution in Florida but is apparentl y not under official control; further introductions an d spread are unlikely to result in the loss of additional foreign markets for domestically produced commodities. Medium (2)

**Risk Element #5: Environmental Impact** As a poly phagous species, C. rusci can attack species listed as Threatened or Endangered (50 CFR §17.12) and present in the continental United States (outside Florida, where it already occurs) that are climatically suitable for this scale species, including Allium munzii, Brodiaea filifolia, B
. pallida, Callirhoe scabriuscula, Calochortus tiburonensis, Calystegia stebbinsii, Cercocarpus traskiae, Chlorogalum purpureum, and Eremalche kernensis (USFWS, 2007). These plants occur in plant families containing host genera for C. rusci ; as preference tests with these plants and C. rusci are unknown, we assume C. rusci could use one or more of these plants as hosts. High (3)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 38 **Ceroplastes rusci Maskell (Hemiptera: Coccidae)**  **Risk r**  **ating s**  Considering it is an important economic pest, biological or chemical control may be impl emented if introduced into the United States. **Conogethes punctiferalis (Guenée) (Lepidoptera: Crambidae)**  **Risk r**  **ating s**  **Risk Element #1: Climate/Host Interaction** Conogethes punctiferalis is found in Australia (Northern Territory , Queensland, northern New South Wales) India; China (Anhui, Fujian, Guangdong, Guangxi, Hebei, Henan, Hubei, Jiangsu, Jiangxi, Liaoning, Shaanxi, Shandong, Shanxi, Sichuan, Xizhang (Tibet), Yunnan, Zhejiang); Japan; and Indonesia (CABI, 2007; Common, 1990). Based on this , we estimate that C. punctiferalis could bec ome established in Plant Hardiness Zone s 5 - 10 (USDA, 1990). One or more of its potential hosts occurs in these zones ( NRCS , 2007). High (3)

**Risk Element #2: Host Range** Conogethes rubens attack s multiple species in multiple families including: **Anacardiaceae** (

Magniera spp.); **Bixaceae** ( Bixa orellana ); **Bombacaceae** ( Ceiba pentandra, Durio zibethinus ); **Burseraceae** ( Garuga pinnata ); **Compositae** ( Helianthus annuus ); **Euphorbiaceae** ( Endospermum diadenum, Mallotus philippensis, Ricinus spp.); **Fagaceae** ( Castanea sativa ); **Gramineae** ( Andropogon sorghum, Sorghum spp., Zea mays ); **Lecythidaceae** ( Planchonia careya ); **Loranthaceae** ( Dendropthoe glabrescens ); **Malvaceae** ( Gossypium spp.); **Moraceae** (Ficus carica, Morus spp.); **Myrtaceae** (

Psidium spp.); **Pinaceae** ( Abies spp., Cedrus deodara, Pinus spp.); **Proteaceae** (

Macadamia spp.); **Rosaceae** ( Eriobotrya japonica , Malus pumila, Prunus persica, Pyrus communis ); **Rubiaceae** ( Mitragyna diversifolia, Morinda citrifolia, Nauclea orientalis ); **Rutaceae** ( Citrus spp.); **Sapindaceae** ( Dimocarpus longan, Lepisanthes rubiginosa, Nephelium spp.); **Sterculiaceae** ( Theobroma cacao ); **Verbenaceae** ( Tectona grandis ); **Zingiberaceae** ( Alpinia spp., Curcuma spp.) (Robinson et al
. , 2001.) High (3)

**Risk Element #3: Dispersal Potential** Conogethes punctiferalis has high biotic potential , with up to five generations per year (CABI, 2007). . F emale s lay 20 - 30 eggs on the surface of fruits . Eggs hatch 5
- 8 day s after oviposition. The hatching rate may be 100 percent. Like other species of Crambidae, C. punctiferalis is presumed to be capable of long distance flight (Showers et al
. , 2001), although like other Crambidae we assume it would not disperse out of favorable habitats (Cohen et al
. , 2000; Qureshi et al
. , 2005). Additionally , C. puncterferalis c an be dispersed by infested plant materials, such as fruits and seeds. S ince 1985, species of Conogethes have been intercepted at U.S. ports in a variety of commodities , including Prunus , over 700 times ( PestID , 2007). High (3)

**Risk Element #4: Economic Im** **pact** Conogethes punctiferalis is one of the most important insect pests on peaches in southern China , and an important pest on apples in northern China. Infestations cause stunting, scorching and falling of fruit. Conogethes punctiferalis excretions, which cover the fruit surface and have a high sugar content, attract other insect pests and diseases High (3)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 39 **Conogethes punctiferalis (Guenée) (Lepidoptera: Crambidae)**  **Risk r**  **ating s**  which damage fruit (CABI, 2007). In Andhra Pradesh, India, it is a major pest of castor. In Gujarat, India, Kapadia (1996) estimated the percentage weight loss of damaged seeds of castor was 63 percent . In maize and sorghum, pollination is reduced because of damage from C. punctiferalis feeding on tassels and maize ear silk. Stems bored b y C. punctiferalis are easil y broken by the wind and farming practices, resulting in a decrease in yield. O ver 5 percent maize loss was caused b y C. punctiferalis and Ostrinia furnacalis in Zhejiang, China (CABI, 2007). C. punctiferalis is listed as a regulated/quarantine pest by the European Plant Protection Organization (EPPO) and the Canadian Food Inspection Agency (CFIA) (CFIA, 2007; EPPO, 2000b). Its presence in the United States, even as a temporary adventive population, could lead to severe export restrictions of host commodities to these and other markets outside of this pest’s known distribution. **Risk Element #5: Environmental Impact** Conogethes punctiferalis could damage Federall y listed threatened and endangered species, such as Prunus genuclata (FL). This species is closely related to other hosts known to be attacked b y C. punctiferalis (USFWS, 2007). Because it represents a potential threat to the Citrus, stone fruit, and other important crop industries, the establishment of C. punctiferalis in the United States coul d trigger the initiation of eradication or chemical/biological control programs, similar to those that have occurred elsewhere (CABI, 2007). High (3)

**Cryptoblabes gnidiella (Lepidoptera: Pyralidae)**  **Risk r**  **ating s**  **Risk Element #1: Climate/Host Interaction** This is a cosmopolitan species in warm climates, unable to survive winters in cooler temperate areas (CABI, 2007). It is distributed south of Western Europe (France, Austria), the Middle East (Turkey , Israel), South Asia, Northern Africa, Uruguay , and New Zealand (CABI, 2007). Establishment is possible in Plant Hardiness Zones 7
- 11 (USDA, 1990). High (3)

**Risk Element #2: Host Range** Cryptoblabes gnidiella is poly phagous , and often encountered on commercial crops (CABI, 2007). Hosts include citrus ( Citrus spp.), avocado ( Persea americana ), grapes ( Vitis spp.), pomegranate ( Punica granatum ), rice ( Oryza sativa ), cotton ( Gossypium spp.), garlic (

Allium sativum ), beans (

Phase olus spp.), coffee ( Coffea spp.), maize (Zea mays ), mango (

Mangifera indica ), and sugarcane (Saccharum officinarum ) (CABI, 2007). High (3)

**Risk Element #3: Dispersal Potential** About 100 eggs per female are laid on the fruit or foliage and hatch in 4
- 7 day s. There are three or four generations per year in Southern Europe and up to five in North Africa (CABI, 2007). Larvae are internal feeders of fruits or stems (CABI, 2007), therefore, their dispersal is possible with the commodities and plant material. Lik e other Py ralids, adults are capable of flight and may disperse long distances. High (3)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 40 **Cryptoblabes gnidiella (Lepidoptera: Pyralidae)**  **Risk r**  **ating s**  **Risk Element #4: Economic Impact** Cryptoblabes gnidiella is an important pest of citrus ( Citrus spp.), grapes ( Vitis spp.) and pomegranates (

Punica granatum ) in the M editerranean (CABI, 2007). It is most noted as a pest of avocado ( Persea americana ) in Israel, of azolla ( Azolla sp.), sorghum (Sorghum spp.) and rice ( Oryza sativa ) in India, and sporadicall y of corn (Zea mays ) or other crops in any warm part of the world (CABI, 2007). The losses caused by this pest have not been quantified. Because C. gnidiella affects some important export crops (including citrus, cotton, beans, grapes, etc.) its presence in the United States, even as a temporary adventive population, could lead to severe export restrictions of host commodities to markets outside of this pest’s known distribution. High (3)

**Risk Element #5: Environmental Impact** Chemical and biological control are likel y to be implemented after introduction of this pest. For example, s ynthetic pyrethroids as well as biological control agents such as Bacillus thuringiensis var. kurstaki are useful against this pest (CABI, 2007). As a poly phagous insect, it is potentially capable of attacking Endangered and Threatened speci es from the genus Allium (USFWS, 2007). High (3)

**Deudorix epijarbas (Moore)**  **(Lepidoptera: Lycaenidae** **) Risk**  **r ating**  **s Risk Element #1: Climate/Host Interaction**  Deudorix epijarbas is found in several countries in southeast Asia and Oceania, including: China (Guangdong, Hainan, Hong Kong), Fiji, India (Himachal Pradesh, Jammu & Kashmir, Uttar, Uttaranchal), Indonesia, Laos, Malay sia, the Philippines, Sri L anka, Taiwan, Thailand, Vietnam, Australia (Queensland), New Guinea, Solomon I slands, and Samoa (CAB I, 2007; Common and Waterhouse, 1972; DAFF, 2004; Marsh, 1960; Waite and Hwang, 2002; Zaka
- ur - Rab, 1980). Based on this distribution we estimate that D. epijarbas could establish in Plant Hardiness Zone s 7 - 10 (USDA, 1990) . Several hosts and potential hosts of D. epijarbas are present in these zones ( NRCS , 2007). High (3)

**Risk Element #2: Host Range** Deudorix epijarba attack s multiple species in multiple families including: **Arecaceae** ( Caryota ), **Celastraceae** (Salacia ), **Connaraceae** ( Connarus ), **Elaeocarpaceae** ( Elaeocarpus ), **Proteaceae** ( Macadamia ), **Punicaceae** (Punica ), **Sapindaceae** ( Dimocarpus, Harpullia, Litchi, Nephelium, Pometia, Sapindus, Sarcopteryx) (CABI, 2007; Common and Waterhouse, 1972; Herbison
- Evans and Crossley , 2002; Savela, 2007; Waite and Hwang, 2002). High (3)

**Risk Element #3: Dispersal Potential** Very little is known about the biology of this pest. One report indicates that females can lay up to four eggs inside the cal yxcup of pomegranate fruit (Zaka
- ur- Rab, 1980). As a congener of D. isocrates , we expect that D. epijarbas females can lay several dozen eggs during their lifetime (Karuppuchamy et al
. , 1998). Because this pest, has 3
- 4 g enerations per year (Verma, 1985), we consider that its reproductive potential is high. High (3)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 41 **Deudorix epijarbas (Moore)**  **(Lepidoptera: Lycaenidae** **) Risk**  **r ating**  **s** Lycaenidae are strong, rapid fliers (Borror et al
. , 1989), and in some cases, dispersal may be aided by

wind (Robbins and Small Jr., 1981). Deudorix spp. can also be artificially

spread in infested fruit (Waite and Hwang, 2002; W ynter- Bl yth, 1957). **Risk Element #4: Economic Impact** Deudorix epijarbas is a serious pest of pomegranates (Zaka
- ur- Rab, 1980). Larvae bore into fruit, feeding on the seeds and their arils (Verma, 1985; Zaka
- ur - Rab, 1980). The accumulation of opportunistic fungi on the resulting wounds causes the fruit to rot (Zaka
- ur- Rab, 1980). In the Jammu region of India, D. epijarbas caused immature pomegranate fruit to drop and damaged an average of 25 percent of the fruit that remained on the tree (Thakur et al
. , 1995). In the Himachal Pradesh region, pomegranate infestation varied from 8 to 96 percent during the active months of May to October (Prasad et al
. , 1987). Similar levels of damage occur red elsewhere in India (e.g., Zaka
ur- Rab, 1980). In addition, D. epijarbas infestations indirectly impact when growers use chemical and biological control programs ( e.g . , Dubey et al
. , 1993; Mani et al
. , 2005). Consequently , this pest poses a significant economic threat to producers of pomegranates and other large
- seeded fruit, particularly in arid regions. With the exception of macadamia nuts, the United States is not a major producer or exporter of any commodities known to be hosts of this pest. P roduction of macadamia nuts in the United States occurs almost exclusively in Hawaii . Thus , the establishment of this pest in the conterminous United States is unlikely would lead to a loss of foreign markets or increased trade restrictions. Medium (2)

**R isk Element #5: Environmental Impact**  Compared to other pests, D . epijarbas has a restricted host range and feeds on few plants found in the United States. This relative oligophagy suggests that this species does not constitute a significant threat to threatened or endangered plants in the United States ; n o Federal Threatened or Endangered species are congeners of reported hosts (USFWS, 2007) . The introduction of Deudorix epijarbas into the United States may lead to the initiation of chemical or biological c ontrol programs, which could have indirect environmental impacts. Medium (2)

**Deudorix isocrates (Fabricius)**  **(Lepidoptera: Lycaenidae** **) Risk**  **r ating**  **s Risk Element #1: Climate/Host Interaction**  Deudorix isocrates has been reported from Bangladesh and I ndia , below 8000 feet elevation (CABI , 2007; Wy nter- Bly th, 1957). W e estimate that D. isocrates could become established in Plant Hardiness Zone s 8 - 10 (USDA, 1990) , where s everal hosts and potential hosts of D. isocrates are present ( NRCS , 2007). Medium (2)
**Risk Element #2: Host Range** D. isocrates attack s multiple species in multiple families including: **Anacardiaceae** ( Mangifera ); **Euphorbiaceae** (Emblica ); **Fabaceae** ( Tamarindus ); **Loganiaceae** (Strychnos ); **Myrtaceae** ( Psidium ); **Punicaceae** (Punica ); **Rhamnaceae** (Ziziphus ); **Rosaceae** (

Eriobotrya , Malus , Prunus, Pyrus ); **Rubiaceae** ( Gardenia, Randia ); **Rutaceae** ( Citrus ); **Sapindaceae** ( Litchi); and **Sapotaceae** (Sapota ) (CABI, 2007; High (3)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 42 **Deudorix isocrates (Fabricius)**  **(Lepidoptera: Lycaenidae** **) Risk**  **r ating**  **s** Srivastava, 1997; Wy

nter - Bl yth, 1957). **Risk Element #3: Dispersal Potential** Deudo rix isocrates has a relativel y high biotic potential, producing up to four generations per year on pomegranate in India (Karuppuchamy et al
. , 1998). Depending on climate and host conditions, females can lay 6 - 46 eggs with a mean generation time of 45 day s. Females lay

eggs singly

or in groups of 2 to 7 eggs, on the flowers, fruit, leaves, stem, and stalk (Karabhantanal, 2005; Karuppuchamy et al
. , 1998; Srivastava, 1997 ). P opulations can increase b y a factor of 1.5 each week, and double every 11 day s (Sheval e, 2003). Deudorix isocrates is also assumed to have a moderate to high dispersal potential. Lycaenidae are strong, rapid fliers (Borror et al
. , 1989; Wy nter- Bly th, 1957), and dispersal may sometimes be aided b y wind (Robbins and Small Jr., 1981). Deudorix spp. can also spread in infested fruit (Waite and Hwang, 2002; Wy nter - Bl yth, 1957). High (3)

**Risk Element #4: Economic Impact** Deudorix isocrates is a serious pest of some fruits, especiall y pomegranate, guava, and aonla (Haseeb, 2007; Srivastava and Jain, 1973; Wy nter- Bl yth, 1957). It is unknown how serious it is on citrus. Larvae bore into fruit, feeding on the flesh and seeds. This reduce s crop yi eld (Haseeb, 2007) and also attracts bacteria and fungi whose growth produces a bad smell in the fruit , making it unmarketable (Karabhantanal, 2005). Larval feeding deforms and weakens fruit, promotes decay , and leads to fruit drop (Haseeb, 2007). In Tamil Nadu, India, D. isocrates damaged 38.5 percent of the pomegranate fruit at harvest (Murugan and Thirumurugan, 2001). On aonla in MadhyaPradesh, India, D. isocrates damaged more than 70 percent of the fruit (Srivastava and Jain, 1973). On guava in Uttar Pradesh, D
. isocrates attacked 2.5
- 23 percent of the crops, resulting in 5
- 35 percent yi eld loss (Haseeb , 2007). Finally , on orange in India, it damaged 4.5 percent of the fruit (Verma et al
. , 1995). Establishment of D. isocrates in the United States could lead to a loss of foreign and domestic markets in countries that regulate this pest. One important U. S. export that could be affected is citrus. High (3)

**Risk Element #5: Environmental Impact** Deudorix isocrates may

directly impact Federal listed endangered species such as Prunus geniculata (FL) and Ziziphus celata (FL) , which are closel y related to other hosts known to be attacked by D. isocrates (USFWS, 2007). Because it represents a potential threat to the Citrus, stone fruit, and other crop industries, the establishment of D. isocrates in the United States could tri gger the initiation of eradication or chemical/biological control programs, similar to those that have occurred in Hawaii and California, and Florida for other Tephritid fruit flies (CABI, 2007; Clausen, 1978). High (3)

**Dichocrocis megillalis (Lepidoptera: Pyralidae)**  **Risk r**  **ating s** Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 43 **Dichocrocis megillalis (Lepidoptera: Pyralidae)**  **Risk r**  **ating s**  **Risk Element #1: Climate/Host Interaction** Dichocrocis megillalis is in several countries in tropical Asia , including Malaysia, West Malay sia, Thailand, Sri Lanka, Indonesia, and India (CABI, 2007; Robinson et al
. , 2007). Consequently , we estimate that it could only establish in Plant Hardiness Zone 10 in the United States (USDA, 1990). One or more of its potential hosts occurs in this zone ( NRCS , 2007). Low (1)

**Risk Element #2: Host Range** Dichocrocis megillalis attack s species in several different families including: **Lauraceae** (

Litsea castanea ); **Lythraceae** ( Punica granatum ); **Solanaceae** (Solanum melongena ); **Verbenaceae** ( Tectona grandis ); and **Rubiaceae** ( Uncaria gambier) (CABI, 2007; Robinson et al
. , 2007). High (3 )

**Risk Element #3: Dispersal Potential**  We found v ery little information on the biology of this species. In general , Py ralid moths have a medium to high reproductive potential. We presumed that D. megillalis is capable of long distance flight (Showers et al
. , 2001), although like other Py ralidae we assume it would tend not to disperse out of favorable habitats (Cohen et al
. , 2000; Qureshi et al
. , 2005). Larvae are internal feeders of fruits or stems (Robinson et al
. , 2001) and may

be able to disperse long distances inside infested commodities. High (3)

**Risk Element #4: Economic Impact** Due to the lack of information about this pest, its potential economic impact is very uncertain. Some py

ralid moths have cause d severe economic damage on certain crops. This species seems to have a narrow host range, and is not known to attack any major U.S. export crop. Based on its current geographical distribution, very few areas of the United Sates would be suitable for its establishment. Medium (2)

**Risk Element #5: Environmental Impact**  Dichocrocis megillalis has a restricted host range , which indicates that this species likely i s not a significant threat to threatened or endangered plants in the United States . N o Federal Threatened or Endangered species are congeners of reported hosts (USFWS, 2007) . Still , introduction of this pest into pomegranate
- or date
- producing areas of the United States could initiate chemical or biological control programs. Medium (2)

**Drosicha dalbergiae (Green) (Hemiptera: Margarodidae** **) Risk**  **r ating**  **s Risk Element #1: Climate/Host Interaction**  Drosicha dalbergiae has only been reported from India and Taiwan (Ben
- Dov et al
. , 2007). In India, it occurs in the states of Jammu & Kashmir, and Himachal Pradesh where almonds and pomegranates are grown (Gaffar, 1989; Rawat et al
. , 1989). Based on this , we estimate that this pest could only become established in Plant Hardiness Zones 8 - 10 of the United States (USDA, 1990). One or more of its potential hosts occurs in these Zones (

NRCS , 2007). Medium (2)

**Risk Element #2: Host Range** We found little information about D. dalbergiae . Currently , onl y a few hosts are known: **Anacardiaceae** (

Mangifera ); **Caricaceae** ( Carica ); **Bromeliaceae** ( Ananas ), **Fabaceae** ( Dalbergia ), **Junglandaceae** ( Junglans ), **Myrtaceae** (

Psidium, Syzygium ), **Punicaceae** High (3)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 44 **Drosicha dalbergiae (Green) (Hemiptera: Margarodidae** **) Risk**  **r ating**  **s** ( Punica ), **Rosaceae** (

Malus , Prunus ), **Rutaceae** ( Citrus ), **Sapindaceae** ( Litchi), and **Sapotaceae** (

Achras , Manilkara ) (Ali, 1968; Ben
- Dov et al
. , 2007; Butani, 1993; CABI, 2007; Masoodi et al
. , 1988). **Risk Element #3: Dispersal Potential**  Aside from one report of the biology of D. dalbergiae on almonds (Koul et al
. , 2000), we found no information on the reproductive and dispersal potential of D. dalbergiae . Gravid females lay

approximately 100
- 150 eggs in a cluster; occa sionally, they may produce 2
- 3 egg clusters (Koul et al
. , 2000). After eclosing, the first instar ny mphs feed on their host’s R s, and then overwinter in the soil. Once their host becomes phenologically active in the spring, ny mphs emerge, continue feeding, and complete their development (Koul et al
. , 2000). Other Drosicha species feed on their hosts, lay eggs in the soil, overwinter in the soil, and return to their hosts when conditions become favorable again (Arkhangel'Skaya, 1931; Prasad and Singh, 1976; Rahman, 1939). Because it apparently produces only one generation per year, we estimate D . dalbergiae ’s reproductive potential to be medium . Drosicha dalbergiae is somewhat mobile; all life stages are able to move within their host, and between their host and the soil. However, because the first instars emerge underground, feed underground or near the surface where they will not be exposed to high winds, we do not expect them to readily undergo long
- distance dispersal by wind. It s restricted global distribution indicates this species probably has a relatively

limited dispersal potential. Medium (2)

**Risk Element #4: Economic Impact** We found little information about the economic impact of D
. dalbergiae. It attacked 25 percent of pomegranate fruit (Rawat et al
. , 1989). On almonds, it reduces nut yield and quality (Koul et al
. , 2000). Because of its impact, researchers are try ing to identify

suitable pesticides to control this pest (Gaffar, 1989). In general, Drosicha species attack R s, stems, shoots, leave s, and fruit of hosts (Masoodi et al
. , 1988; Rawat et al
. , 1989; Vijay et al
. , 2000b). Drosicha corpulenta causes serious damage in China (Qu et al
. , 1996) , a nd D. mangiferae causes considerable damage to several economically

important fruit trees, including mangoes (Lakra et al
. , 1980; Prasad and Singh, 1976). Establishment of D. dalbergiae in the United States may result in a loss of some foreign and domestic markets, or at the very least, in trade restrictions. High (3)

**Risk Element #5: Environmental Impact** Because we know so little about D. dalbergiae , including its host range, we do not know which federall y Threatened and Endangered (T&E) species it may harm. Based on it s currently known host range, it may affect one T&E species in Florida, Prunus geniculata (FL) (USFWS, 2007). Because the genus Drosicha does not occur in the United States (Arnett Jr., 2000), its introduction may necessitate the use of pesticides to control it, as elsewhere (Gaffar, 1989; Patyal and Nath, 1993). Because of its potential impact on Prunus geniculata and the uncertainty described above, we gave a risk rating of High. High (3)

**Dysmicoccus neobrevipes Beardsley (Hemiptera: Pseudococcidae)**  **Risk r**  **ating s** Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 45 **Dysmicoccus neobrevipes Beardsley (Hemiptera: Pseudococcidae)**  **Risk r**  **ating s**  **Risk Element #1: Climate/Host Interaction** Dysmicoccus neobrevipes occurs throughout Central America, northern South America, the Caribbean, Indo
- China, the Philippines, and Oceania (Ben
- Dov et al
. , 2007). Within the United States , it occurs in Florida, Puerto Rico, American Samoa, and Hawaii (Miller, 2005; Miller and Miller, 2002). These regions roughl y correspond to Plant Hardiness Zones 9
- 10 (Maxwell, 2007; Widrlechner, 1997). Thus, o utside of greenhouse or other artificial situ ations, this species should be able to survive in the warmer, southern United States. One or more hosts of D. neobrevipes are present in these zones ( NRCS , 2007). Medium (2)

**Risk Element #2: Host Range** Host species of D. neobrevipes include the following plant families and genera: **Agavaceae** (

Agave, Polianthes, Yucca ), **Anacardiaceae** ( Mangifera ), **Annonaceae** ( Annona ), **Apocynaceae** ( Plumeria ), **Araceae** (

Aglaonema, Colocasia, Philodendron, **Araliaceae** (

Nothopanax ), **Arecaceae** ( Chrysalidocarpus, Cocos ), **Asteraceae** ( Helianthus ), **Balsaminaceae** ( Impatiens ), **Barringtoniaceae** ( Barringtonia ), **Bignoniaceae** ( Crescentia, Millingtonia, Spathodea ), **Boraginaceae** ( Cordia , Messerschmidia, Tournefortia, **Bromeliaceae** ( Ananas ), **Brassicaceae** (

Brassica ), **Cactaceae** ( Opuntia ), **Clusiaceae** ( Garcinia ), **Cucurbitaceae** ( Cucurbita ), **Euphorbiaceae** ( Codiaeum ), **Heliconiaceae** ( Heliconia ), **Fabaceae** (

Acacia, Albizia, Arachis, Cajanus, Machaerium, Phaseolus, Piscidia, Samanea, Tamarindus, Vigna ), **Lauraceae** (

Persea ), **Liliaceae** (

Allium ), **Malvaceae** ( Gossypium, Thespesia ), **Meliaceae** (

Lansium ), **Moraceae** (

Artocarpus, Ficus ), **Musaceae** ( Musa ), **Myrtaceae** ( Psidium, Syzygium ), **Orchidaceae** ( Brassavola ), **Pandanaceae** (Pandanus ), **Pinaceae** ( Pinus ), **Poaceae** (Zea ), **Polygonaceae** ( Coccoloba ), **Punicaceae** ( Pu nica ), **Rubiaceae** ( Coffea, Guettarda, Mussaenda ), **Rutaceae** ( Citrus ), **Sapindaceae** ( Nephelium ), **Sapotaceae** (

Manilkara ), **Solanaceae** ( Lycopersicon, Solanum ), **Sterculiaceae** ( Theobroma ), **Urticaceae** ( Pipturus ), **Verbenaceae** ( Clerodendrum, Tectona ), **Zingiberaceae** (

Alpinia ) (Ben
- Dov et al
. , 2007; Chia and Evans, 1997). High (3)

**Risk Element #3: Dispersal Potential** The life span of D. neobrevipes ranges from 59 to 117 day s, averaging 90 day s (Martin
Kessing and Mau, 2007). This mealy bug is ovoviviparous, meaning the eggs hatch within the female; females produce about 350 larvae over 30 day s, but some produce up to 1000 larvae (Martin
- Kessing and Mau, 2007). There are three instars for females, and four for males. The total larval period for females varies from 26 to 5 2 day s, averaging 35 day s; the larval period for males lasts from 22 to 53 day s. The primary dispersal stage is the larval stage. Dysmicoccus neobrevipes can be dispersed b y wind (Martin
Kessing and Mau, 2007). Lo ng distance dispersal is accomplished via the movement of infested plant materials. S ince 1985, inspectors at U.S. ports
- of
- entry have intercepted D. neobrevipes 3,100 times on fruit, 100 of which were in permit cargo ( PestID , 2007). High (3)

**Risk Element #4: Economic Impact** Dysmicoccus neobrevip es attacks numerous commercial crops, such as pineapple, citrus, banana, coffee, and coconut (Williams, 2004) . Itis one of the most economically important mealy

bug pests in Hawaii because it vector s meal ybug wilt on pineapples (Sether et al
. , 1998) . This is the most serious ty pe of damage, and is the principal cause of crop failure in Hawaii (Martin
- Kessing and Mau, 2007). Feeding b y mealybugs may Medium (2)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 46 **Dysmicoccus neobrevipes Beardsley (Hemiptera: Pseudococcidae)**  **Risk r**  **ating s**  cause a loss of host plant vigor or die
- back of plant organs (Martin
- Kessing and Mau, 2007; Williams, 2004 ). Consequently, any country that grows pineapples would most likely consider D. neobrevipes to be a quarantine pest. Our literature review showed that D. neobrevipes is primarily known for damaging pineapples and may not be an economicall y important pest of other fruits and vegetables. **Risk Element #5: Environmental Impact** It is unknown if establishment of D. neobrevipes would reduce community biodiversity

or disrupt ecological processes in natural areas. Pest interception data suggest that Pinus may be host for this pest (p. 206; Williams, 2004). If Pinus is indeed a good host for this pest, then D. neobrevipes may

have a significant environmental impact in the continental United S tates because pine trees are an important component of many p lant communities, particularly

in the southeast (Myers, 1990). Dysmicoccus neobrevipes may affect several F ederal Threatened and Endangered (T&E) species (ouside of Floirda and Hawaii, where it is already present) that are congeners of current hosts, including: Allium munzii (CA), Helianthus paradoxus (NM, TX), H
. schweinitzii (NC, SC), and Opuntia treleasei (CA) (USFWS, 2007). Because this pest only seems to be economically important on pineapple, the wider establishment of this species in the continental United States would not likely stimulate the initiation of chemical or biological control programs or increase the use of chemical pesticides already in place to control other scale species. Medium (2)

**Euzophera bigella (Lepidoptera: Pyralidae)**  **Risk r**  **ating s**  **Risk Element #1: Climate/Host Interaction** Euzophera bigella is currently distributed through out the Mediterranean, Europe from UK to Republic of Georgia, Turkmenistan, Uzbekistan, Iran, and Central Asia (Zhang, 1994). Based on this distribution we estimate that E. bigella could only establish in Plant Hardiness Zone s 8 - 10 in the United States (USDA, 1990). One or more of its hosts occurs in this zone (

NRCS , 2007). Medium (2)

**Risk Element #2: Climate/Host Interaction**  Euzophera bigella has been reported on several different species in several different families. Reported hosts include: **Juglandaceae** ( Juglans regia ), **Lythraceae** ( Punica granatum ), **Rosaceae** (

Malus , Osteomeles , Prunus ), **Vitaceae** ( Vitis ) (Robinson et al
. , 2001; Robinson et al
. , 2007) High (3)
**Risk Element #3: Dispersal Potential** We found little information on the biology of this species. Py

ralid moths generall y have a medium to high reproductive potential and a high dispersal potential . We presume that E
. bigella , l ike other Py

ralidae, is capable of long distance flight (Showers et al
. , 2001), but would tend not to disperse out of favorable habitats (Cohen et al
. , 2000; Qureshi et al
. , 2005). Larvae are internal feeders of fruits or stems (Nazeer Ahmad, 1942) and therefore may dispers e via commodities and plant material. High (3)

**Risk Element #4: Economic Impact** Due to the lack of information about this pest, its potential economic impact is very uncertain. Euzophera bigella attacks several important U.S. crops, including the major export crops, peache s, apples, and grapes . Some py ralid moths have cause d severe High (3)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 47 **Euzophera bigella (Lepidoptera: Pyralidae)**  **Risk r**  **ating s**  economic damage on certain crops. Establishment of this pest in the United States may result in a loss of some foreign and domestic markets, or at the very least, in trade restrictions. **Risk Element #5: Environmental Impact** Euzophera bigella may

have direct impacts on Federal listed endangered species such as Prunus geniculata (in Florida), which is closel y related to other hosts known to be attacked b y B. zonata (USFWS, 2007). Because it is a potential threat to grapes, stone fruit, and other crop industries, the establishment of E. bigella in the United States could initiat e eradication or chemical/biological control programs, similar to those that have occurred for other moths in California. High (3)

**Icerya aegyptiaca (Douglass) (Hemiptera: Margarodidae** **) Risk**  **r ating**  **s Risk Element #1: Climate/Host**  **Interaction** Icerya aegyptiaca is distributed in western and eastern Africa, tropical Asia, Australia, and numerous South Pacific islands (CABI, 2007). These regions roughly correspond to Plant Hardiness Zones 10
- 11 (Maxwell, 2007; Widrlechner, 1997). We e s timate I. aegyptiaca could only

survive in the continental United States in Zone 10. One or more of its potential hosts occurs in these zones ( NRCS , 2007). Low (1)

**Risk Element #2: Host Range** Icerya aegyptiaca has a wide variety of hosts, especiall y woody plants. The host list includes, but is not limited to , the following families and genera: **Acanthaceae** ( Asystasia , Barleria , Barleria , Eranthemum 36**,** Justicia **,** Pseuderanthemum , Sanchezia, Strobilanthes, Thunbergia ), **Alangiaceae** ( Alangium ), **Am aranthaceae**  (Bragantia ), **Anacardiaceae** (

Mangifera , Schinus ), **Annonaceae** ( Annona , Polyalthia ), **Apocynaceae** ( Carissa , Holarrhena , Nerium , Ochrosia ), **Araceae** ( Colocasia , Cyrtosperma , Monstera ), **Arecaceae** ( Caryota , Cocos , Hyophorbe , Latania , Phoenix , Rhapis ), **Aristolochiaceae** (Aristolochia) , **Asclepiadaceae** ( Cryptostegia ), **Asteraceae** ( Aster , Bidens , Pluchea , Synedrella , Vernonia ), **Blechnaceae** ( Blechnum ), **Bombacaceae** ( Ceiba , Cullenia ), **Boraginaceae** ( Cordia ), **Caricaceae** ( Carica ), **Casuarinaceae** ( Casuarina ), **Clusiaceae** ( Calophyllum , Hypericum ), **Convolvulaceae** ( Ipomoea ), **Cupressaceae** ( Thuja ), **Cyatheaceae** ( Cyathea ), **Euphorbiaceae** ( Acalypha , Aleurites , Codiaeum , Croton , Drypetes , Euphorbia , Glochidion , Jatropha , Macaranga , Vemicia ), **Fabaceae** (

Acacia , Alhagi, Caesalpinia, Cajanus , Cassia , Dalbergia , Genista , Indigofera , Jacaranda , Parkinsonia , Pithecolobium , Poinciana , Vigna ), **Flacourtiaceae** ( Oncoba ), **Flagellariaceae** ( Flagellaria ), **Goodeniaceae** (Scaevola ) , **Lamiaceae** ( Coleus , Lavandula , Leucas , Ocimum , Rosmarinus ), **Lauraceae** ( Cinnamomum , Laurus , Persea ), **Liliaceae** (

Asparagus ), **Lythraceae** ( Heimia , Lawsonia , Nesaea , Pemphis ), **Malpighiaceae** (

Malpighia ), **Malvaceae** ( Hibiscus ), **Meliaceae** (

Melia ), High (3)

36 Current name of the genus DaedalacanthusPest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 48 **Icerya aegyptiaca (Douglass) (Hemiptera: Margarodidae** **) Risk**  **r ating**  **s Melostomataceae**  (

Memecylon ), **Montiniaceae** ( Montinia ), **Moraceae** (

Artocarpus , Ficus , Morus ), **Musaceae** ( Musa ), **Myrtaceae** (

Eugenia , Myrtus , Psidium , Syzygium ), **Oleaceae** (

Jasminum ), **Onagraceae** ( Ludwigia ), **Pandanaceae** ( Pandanus ), **Piperaceae** ( Piper) **Pittosporaceae** (Pittosporum ), **Plumbaginaceae** ( Plumbago ), **Poaceae** (Zea ), **Polemoniaceae** (Pterospermum ), **Punicaceae** ( Punica ), **Rhamnaceae** (

Rhamnus , Ziziphus ), **Rosaceae** (

Eriobotrya , Malus , Osteomeles , Prunus , Pyrus , Raphiolepis , Rosa ), **Rubiaceae** ( Coffea , Psychotria , Rubia ), **Rutaceae** ( Bonin ia , Citrus , Glycosmis ), **Salicaceae** (Salix), **Sapindaceae** ( Dodonaea ), **Sapotaceae** ( Achras , Manilkara , Pouteria , Sapota , Sideroxylon ), **Solanaceae** ( Capsicum , Lycopersicon , Solanum ), **Theaceae** ( Camellia , Thea ), **Tiliaceae** ( Corchorus ), **Ulmaceae** ( Trema ), **Verbenaceae** ( Carypoteris , Lantana , Petrea , Tectona ), **Vitaceae** ( Vitis ) (Azab et al
. , 1968; Ben - Dov et al
. , 2007; CABI, 2007). **Risk Element #3: Dispersal Potential** Icerya aegyptiaca has high reproductive potential. Females are parthenogenetic and produce from 70 to 143 eggs per female (Azab et al
. , 1968; CABI, 2007; Waterhouse, 1991). With an average life cycle of 105 day s, there are two to three generations per ye ar (Azab et al
. , 1968). Very little information is available on the dispersal potenti al of I. aegyptiaca . One source indicates that ny mphs are very

active (INKTO/PINKTO, 2002). Li ke its congener, I. seychellarum , we assum e that crawlers move locally within a host or can be transported by wind or other animals to more distant hosts (Hill, 1 980). The most likely

means of long
- distance dispersal in I. aegyptiaca is on nursery plants in trade or resale. Accordingl y, it has spread to many of the tropical regions of the world. Since 1985, this pest has been intercepted 9 times at U.S. ports
- of
- entry on a variety of hosts ( PestID , 2007). High (3)

**Risk Element #4: Economic Impact** Because of its broad host range, I
. aegyptiaca could impact many economically important tropical tree species. It is a major pest of guava ( Psidium guajava ), citrus ( Citrus spp.), breadfruit (

Artocarpus altilis ), jackfruit ( Artocarpus heterophyllus ), mango ( Mangifera indica ), sapodilla ( Manilkara zapota ), and mora ( Morus alba ) (CABI, 2007). This species primarily attacks leaves, but can also infest stems and fruit ( Pes tID , 2007; CABI, 2007). Feeding b y large populations of the meal ybug severe ly deplet e sap, defoliat e , and desiccat e younger stems (Azab et al
. , 1968). On breadfruit, heav y infestations can kill mature trees, but typically reduce yi elds b y 50 percent (Water house, 1991). Honeydew excretions produced by I. aegyptiaca support sooty molds that can interfere with photosy nthesis (Azab et al
. , 1968). D espite these impacts, we do not expect this pest to have a high economic impact in the United States. Inits native range in Indo
- China, it is seldom a pest (Waterhouse, 1991). In other places ( e.g ., Australia), it is not regarded as a serious pest, presumably because natural enemies control it (CABI, 2007; Hill, 1983; Waterhouse, 1991). Icerya aegyptiaca onl y appears to become problematic when populations of its natural enemies perish ( e.g ., Pacific atolls; Waterhouse, 1991). Because several natural enemies of Icerya have already

been introduced into the United States to control I. purchasi , it seems unlikely

that establishment of I. aegyptiaca would have a major economic impact. Medium (2)

**Risk Element #5: Environmental Impact** Medium (2)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 49 **Icerya aegyptiaca (Douglass) (Hemiptera: Margarodidae** **) Risk**  **r ating**  **s** It is unknown if establishment of I. aegyptiaca would reduce community biodiversity

or disrupt ecological processes in natural areas. As a highly poly phagous pest, I. aegyptiaca may affect F ederal Threatened and Endangered (T&E) species that are congeners of current hosts. These include Euphorbia telephioides (FL), Hypericum cumulicola (FL), Justicia cooleyi (FL), Prunus genicu lata (FL), and Ziziphus celata (FL) (USFWS, 2007). Because effective biocontrol agents such as Rodolia cardinalis and Cryptochaetum iceryae are already in the United States, to control populations of I
. purchasi (CABI, 2007; Waterhouse, 1991), it is unlikely that establishment of I. aegyptiaca would stimulate use of additional pesticides and biological control agents. We rated this risk element Medium because of the potential impact on T&E species. **Icerya seychellarum (Westwood) (Hemiptera: Margarodidae** **) Risk**  **r ating**  **s Risk Element #1: Climate**  **- Host Interaction**  Icerya seychellarum is distributed throughout southeast Asia, eastern and southern Africa, Australia, and Oceania (CABI, 2007). These regions roughly correspond to Plant Hardiness Zones 9
- 11 (Maxwell, 2007; Widrlechner, 1997). We e stimate I. seychellarum could survive in the continental United States in Zones 9
- 10. One or more of its potential hosts occurs in these zones in the United States ( NRCS , 2007). Medium (2)

**Risk Element #2: Host Range** Icerya seychellarum has a wide variety of hosts, especially

woody plants. The extensive host list includes, but is not limited to , the following families and genera: **Am aranthaceae**  (

Achyranthes ), **Anacardiaceae** ( Mangifera , Rhus , Semecarpus , Spondias ), **Annonaceae** ( Annona, Monodora ), **Apiaceae** (Schefflera ), **Apocynaceae** ( Alyxia , Plumeria ), **Araceae** ( Alocasia , Anthurium , Epipremnum , Monstera , Scindapsus , Xanthosoma ), **Arecaceae** ( Areca , Cocos , Elaeis , Livistona , Phoenix), **Aspleniaceae** ( Asplenium ), **Asteraceae** (Bidens, Gerbera , Lactuca , Zinnia ), **Bixaceae** ( Bixa ), **Bombacaceae** ( Ceiba ), **Boraginaceae** ( Cordia ), **Brassicaceae** ( Raphanus ), **Burseraceae** ( Canarium ), **Canellaceae** ( Cinnamomum ), **Caricaceae** ( Carica ), **Casua rinaceae**  ( Casuarina ), **Celastraceae** ( Maytenus , Mystroxylon ), **Clusiaceae** ( Calophyllum , Garcinia, Mammea ), **Combretaceae** ( Lumnitzera , Terminalia ), **Convolvulaceae** ( Convolvulus , Ipomoea, Merremia ), **Cycadaceae** ( Cycas ), **Dioscoreaceae** (

Dioscorea ), **Dracaenaceae** ( Cordyline, Dracaena ), **Elaeocarpaceae** ( Elaeocarpus ), **Euphorbiaceae** ( Acalypha , Croton , Euphorbia, Excoecaria, Glochidion, Macaranga, Margaritaria, Phyllanthus ), **Fabaceae** ( Acacia , Albizia , Bauhinia , Caesalpinia , Cajanus , Cassia , Crotalaria , Derris , Dichrostac hys , Erythrina , Gliricidia , Inocarpus , Mimosa , Phaseolus , Samanea, Vigna ), **Fagaceae** ( Quercus ), **Fl acourtiaceae**  ( Flacourtia , Hydnocarpus ), **Gesneriaceae** ( Cyrtandra ), **Goodeniaceae** (Scaevola ), **Heliconiaceae** (

Heliconia ), **Icacinaceae** ( Apodytes ), **Lamiaceae** ( Ocimum ), **Lauraceae** ( Cinnamomum , Litsea , Persea ), **Lecythidaceae** (Barringtonia ), **Loganiaceae** ( Fagraea ), **Lythraceae** (

Lagerstroemia , Pemphis, Sonneratia ), **Magnoliaceae** ( Magnolia ), **Malvaceae** ( Gossypium , Hibiscus, Thespesia ), **Meliaceae** ( Dysoxylum ), **Menispermaceae** ( Coc culus ), **Moraceae** ( Artocarpus , Broussonetia , Ficus , Morus , Streblus ), **Musaceae** (

Musa ), **Myricaceae** (

Myrica ), **Myrtaceae** (

Eugenia , Feijoa , Psidium , Syzygium ), **Ochnaceae** ( Ochna ), **Oleaceae** ( Jasminum ), **Oleandraceae** High (3)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 50 **Icerya seychellarum (Westwood) (Hemiptera: Margarodidae** **) Risk**  **r ating**  **s** ( Nephrolepis ), **Onagraceae** ( Ludwigia ), **Oxalidaceae** ( Averrhoa ), **Pandanaceae** ( Pandanus ), **Passifloraceae** ( Passiflora ), **Pinaceae** (

Pinus ), **Piperaceae** (Piper), **Poaceae** ( Cymbopogon , Saccharum ), **Po rtulacaceae**  ( Portulacaria ), **Proteaceae** ( Grevillea, Macadamia ), **Punicaceae** ( Punica ), **Rhamnaceae** (

Alphitonia, Scutia ), **Rhizophoraceae** (

Bruguiera , Ceriops, Rhizophora ), **Rosaceae** ( Crataegus , Eriobotrya , Fragaria , Malus , Prunus , Pyrus , Rosa , Rubus ), **Rubiaceae** ( Canthium , Coffea , Coprosma, Guettarda, Ixora, Polysphaeria, Tarenna ), **Rutaceae** ( Citrus , Poncirus ), **Sapinda ceae**  (

Allophylus , Dodonaea , Litchi), **Salvadoraceae** ( Azima ), **Sapotaceae** ( Chrysophyllum , Citharexylum , Sideroxylon , Manilkara ), **Solanaceae** ( Capsicum , Lycopersicon , Solanum ), **Taccaceae** ( Tacca ), **Theaceae** ( Camellia, Thea ), **Verbenaceae** ( Avicennia , Clerodendrum , Premna , Tectona ), **Vitaceae** ( Vitis ), **Zingiberaceae** ( Alpinia, Zingiber) (Ben
- Dov et al
. , 2007; CABI, 2007; Hill and Newberry, 1980; Newbery , 1980a). **Risk Element #3: Dispersal Potential** Development of I. seychellarum usually takes three months (CAB I, 2007). In Japan and South Africa, one generation per year occurs (CABI, 2007; INKTO/PINKTO, 2002) . E lsewhere ( e.g., Aldabra Island) , breeding may be continuous and multiple generations occur per year (Hill and Newberry , 1980). Data on fecundity of this species is not available (CABI, 2007), but the closely related I. aegyptiaca produces 70
- 140 eggs and completes 2
- 3 generations per year in Northern Africa (Azab et al
. , 1968). Males are rare and are not necessary for reproduction (CABI, 2007). All life stages of I. seychellarum retain their legs, and consequently , are mobile to some degree (Hill and Newberry , 1980). The first instar is the most mobile (Hill, 1980). Icerya seychellarum crawlers can move locally within its host or b e transported by wind or other animals to more distant hosts (Hill, 1980). Crawlers have been trapped over water, 3.5 km downwind from an infestation (Hill, 1980). The most likely means of long
- distance dispersal is on nursery

plants in trade or resale. Si nce 1985, this pest has been intercepted 52 times at U.S. ports on a variety of hosts ( PestID , 2007). High (3)

**Risk Element #4: Economic Impact** Because of its broad host range, I
. seychellarum could impact many economically important tropical tree specie s. For example, I. seychellarum has killed trees in the Pacific Islands (CABI, 2007). Itis a pest of guava ( Psidium guajava ), citrus ( Citrus spp.), breadfruit (

Artocarpus altilis ), avocado ( Persea americana ), jackfruit ( Artocarpus heterophyllus ), various genera of palms, and rose ( Rosa spp.) (CABI, 2007). This species primaril y attacks leaves, twigs, and small branches (INKTO/PINKTO, 2002), but it can also be found on fruits, and flowers (PestID , 2007). Feeding decreases plant vigor, reducing L production by as much as 64 percent (Newbery , 1980b). Honeydew excreted b y the scale provides a growth medium for molds, reducing photosy nthesis (INKTO/PINKTO, 2002), and disfiguring fruit. The establishment of this pest in the United States could cause a loss of for eign or domestic markets. High (3)

**Risk Element #5: Environmental Impact** It is unknown if establishment of I. seychellarum would reduce community biodiversity

or disrupt ecological processes in natural areas. Because heavy infestations of I. seychellarum can reduce L growth rates by 52 percent (Newbery, 1980b), widespread infestations of this pest might affect community productivity . F or example, f ollowing establishment on a High (3)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 51 **Icerya seychellarum (Westwood) (Hemiptera: Margarodidae** **) Risk**  **r ating**  **s** small tropical island, populations of I. seychellarum increased to infestation levels, and caused the death of some trees and shrubs (Hill and Newberry, 1980). A few years later, however , pest populations declined for no apparent reason (Hill and Newberry, 1980). As a highly poly phagous pest, I. seychellarum may affect federal Threatened and Endangered (T&E) species that are congeners of current hosts. These include Asplenium scolopendrium var. americanum (AL, MI, NY, TN), Crotalaria avonensis (FL), Euphorbia telephioides (FL), Prunus geniculata (FL), Quercus hinckleyi (TX), and Rhus michauxii (GA, NC, SC, VA) (USFWS, 2007). E stablishment of I. seychellarum in the continental United S tates would likely

result in chemical or biological control, as has happened elsewhere (I NKTO/PI NKTO, 2002; Negm et al
. , 2001) . These programs can have indirect impacts on the environment. Although one natural enemy of I. seychellarum is already present in the United S tates , Rodolia cardinalis , it is unknown how effective this enemy would be in the long run (CABI, 2007). **Maconellicoccus hirsutus (Green) (Hemiptera**  **- Pseudococcidae)**  **Risk r**  **ating s**  **Risk Element #1: Climate -**  **Host Interaction** Maconellicoccus hirsutus is a tropical
- subtropical pest reported in Northern Africa, parts of sub
- Saharan Africa, the Middle East, South and Southeast Asia, the Far East, the Caribbean, Central America, Australia, and Oceania (CABI, 2007). It currently has a limited distribution in the United States, occurring only in Hawaii, California (CABI, 2007), and Florida (Ho y et al
. , 2003). These region s roughl y match Plant Hardiness Zones 9

– 1 3. We estimate that M. hirsutus could establish in Zones 9

– 10 of the continental United S tates , where o ne or more of its potential hosts occurs (NRCS, 2007). Medium (2)

**Risk Element #2: Host Range** Maconellicoccus hirsutus is highly pol yphagous, although it shows some preference for hosts in the families Malvaceae, Fabaceae and Moraceae (CABI, 2007). It feed s on plants from dozens of plant families and genera, including: **Acanthaceae** ( Acanthus, Eranthemum, Pachystachys, Thunbergia ), **Aceraceae** (Saccharum ), **Am aryllidaceae**  ( Calostemma ), **Anacardiaceae** ( Mangifera , Schinus , Spondias ), **Annonaceae** ( Annona, Cananga ), **Apiaceae** (

Angelica , Aralia, Brassaia, Daucus, Eryngium, Schefflera, Sciadophyllum ), **Apocynaceae** ( Acok anthera , Allamanda , Carissa , Nerium, Tabernaemontana, Vinca ), **Araceae** ( Aglaonema , Alocasia , Anthurium , Colocasia , Dieffenbachia , Philodendron , Scindapsus , Syngonium , Xanthosoma ), **Arecaceae** ( Cocos , Phoenix), **Asteraceae** (Bidens , Chrysanthemum , Cosmos , Dahlia , Emilia, Gerbera, Helianthus , Lactuca , Mikania , Parthenium, Symedrella, Tithonia ), **Basellaceae** (

Basella ), **Begoniaceae** (

Begonia ), **Bignoniaceae** ( Bignonia , Crescentia , Jacaranda, Kigelia, Tabebuia, Tecoma ), **Bombacaceae** ( Ceiba ), **Boraginaceae** ( Cordia ), **Brassic aceae**  (Brassica ), **Cactaceae** ( Opuntia, Pereskia ), **Caricaceae** ( Carica ), **Casuarinaceae** ( Casuarina ), **Chenopodiaceae** ( Beta , Chenopodium ), **Combretaceae** ( Quisqualis , Rhoeo , Terminalia ), **Convolvulaceae** ( Ipomoea ), **Crassulaceae** (

Kalanchoe), **Cucurbitaceae** ( Cucumis , C ucurbita ), **Cyperaceae** ( Cyperus ), **Dilleniaceae** ( Tetracera ), **Dioscoreaceae** ( Dioscorea ), **Ebenaceae** ( Diospyros ), **Elaeagnaceae** ( Elaeagnus ), **Ericaceae** (

Rhododendron ), **Euphorbiaceae** ( Acalypha , Codiaeum , Croton , Euphorbia , Hevea , Macaranga , Manihot , Ohyllanthus , High (3)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 52 **Maconellicoccus hirsutus (Green) (Hemiptera**  **- Pseudococcidae)**  **Risk r**  **ating s**  Phyllanthus, Ricinus ), **Fa baceae**  ( Acacia , Albizia , Arachis , Bauhinia , Caesalpinia , Cajanus , Calliandra , Cassia , Ceratonia , Clitoria , Crotalaria , Delonix, Erythrina , Gliricidia , Gliricidium , Glycine , Grewia , Inga , Leucaena , Medicago , Mimosa , Parkinsonia , Phaseolus , Poinciana , Robinia , Samanea , Senna , Sesbania , Tamarindus, Templetonia , Tephrosia, Vigna ), **Fagaceae** ( Pasania, Quercus ), **Flacourtiaceae** ( Flacourtis ), **Gesneriaceae** ( Chrysothemis ), **Heliconiaceae** ( Heliconia ), **Lamiaceae** ( Leonotis ), **Lauraceae** (Persea ), **Lecythidaceae** ( Courouptia ), **Liliaceae** ( Asparagus, Cordyline, Dracaena ), **Lythraceae** ( Lagerstroemia, Lawsonia ), **Malpighiaceae** ( Malpighia ), **Malvaceae** ( Abelmoschus , Abutilon , Althaea , Gossypium , Hibiscus , Holmskia , Malvaviscus, Paritium, Pavonia , Sida , Thespes ia , **Meliaceae** ( Azadirachta, Melia ), **Moraceae** (

Artocarpus , Ficus , Morus ), **Musaceae** ( Musa ), **Myrtaceae** ( Callistemon, Eugenia , Myrtus, Psidium, Syzygium ), **Myrtaceae** ( Psidium , Syzygium ), **Nyctaginaceae** (

Bougainvillea ), **Oleaceae** ( Jasminum ), **Orchidaceae** ( Dendrobium ), **Oxalidaceae** (

Averrhoa ), **Passifloraceae** ( Passiflora ), **Phytolaccaceae** ( Petiveria , Rivina ), **Piperaceae** (

Peperomia, Piper), **Plumbaginaceae** ( Plumbago ), **Poaceae** (Saccharum , Zea ), **Polygonaceae** ( Coccoloba ), **Polypodiaceae** ( Nephrolepis ), **Portulacaceae** (

Portulaca ), **Proteaceae** ( Grevillea ), **Punicaceae** ( Punica ), **Rhamnaceae** ( Colubrina , Ziziphus ), **Rosaceae** ( Crataegus, Cydonia, Eriobotrya , Malus, Prunus, Pyrus, Rosa ), **Rubiaceae** ( Adina , Coffea , Haldina , Hamelia , Ixora , Mussaenda ), **Rutaceae** (Aegle, Citrus, Murraya ), **Salicaceae** (Salix), **Sapindaceae** ( Blighia , Dodonaea, Melicocca ), **Sapotaceae** ( Manilkara ), **Scrophulariaceae** ( Russelia, Scoparia ), **Solanaceae** ( Capsicum , Cestrum , Datura , Lycopersicon, Solanum ), **Sterculiaceae** ( Theobroma ), **Tiliaceae** ( Corchorus , Grewia ), **Urticaceae** ( Boehmeria, Laportea ), **Verbenaceae** ( Clerodendrum , Duranta , Lantana, Petrea, Stachytarpheta, Tectona ), **Vitaceae** ( Cissus , Vitis ), **Zingiberaceae** ( Alpinia ) (Ben
- Dov et al
. , 2007; CABI, 2007). **Risk Element #3: Dispersal Potential** Maconellicoccus hirsutus reproduces via parthenogenesis or biparental mating (CABI, 2007). Each adult female lay s 150 - 600 eggs (CABI, 2007). O ne generation can complete its lifecycle in five weeks under warm conditions, giving multiple generations per year , up to 15 (CABI, 2007). This species moves l ocal ly while in the first instar (crawler) stage (CABI, 2007). Crawlers are very small and light, and are able to survive a day or so without feeding. They are unable to walk far , but are ideally suited for tran sport by

water, wind, and animal agents, including domestic animals and humans (Gullan and Kosztarab, 1997). Accidental introductions into new countries can occur via infested plant material (CABI, 2007). Since 1985, this pest ha s been intercepted on fruit 978 times at U.S. ports - of
- entry , 40 of which were on permit cargo ( PestID , 2007). High (3)

**Risk Element #4: Economic Impact** Direct feeding on young growth by M. hirsutus causes severe stunting and distortion of plant parts , due to its toxic saliva (CABI, 2007; Hill, 1983). Production of honeydew promotes the growth of sooty mold , which reduces plant photosy nthesis and contaminates fruit (CABI, 2007). Itcan stop the growth of cotton in Egy pt, reduce yi eld of tree cotton in India, reduce fiber yield of roselle by 21

–40 percent in Bangladesh, and destroy or contaminate up to 90 percent of grapes in India (CABI, 2007). InGrenada, this pest causes an estimated $3.5 million in annual losses (CABI, 2007). Medium (2)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 53 **Maconellicoccus hirsutus (Green) (Hemiptera**  **- Pseudococcidae)**  **Risk r**  **ating s**  This pest is already present in Florida and not under any apparent official control; it is unlikely that further spread and establishment of this pest in the continental United States would result in additional export restrictions. **Risk Elemen t #5: Environmental Impact**  If M. hirsutus is introduced into the continental United States (beyond Florida and California) it may

affect other Threatened and Endangered plants, particularly those taxa that found in Texas (

Helianthus paradoxus, Manihot walk erae, and Quercus hinckleyi) and the Carolinas ( e . g. , Helianthus schweinitzii) (USFWS, 2007). Continued spread of M. hirsutus in Florida and California, and its establishment in other states such as Texas, could result in additional control measures such as pesticides and biocontrol that could have indirect environmental impacts (Ho y et al
. , 2003). High (3)

**Nipaecoccus viridis (Newstead) (Homoptera: Pseudococcidae)**  **Risk r**  **ating s**  **Risk Element #1: Climate -**  **Host Interaction** Nipaecoccus viridis is widespread in tropical and subtropical Asia, Africa, and in parts of Oceania (Ben
- Dov et al
. , 2007; CABI , 2007). These regions roughly correspond to Plant Hardiness Zones 9
- 12 (e.g., Widrlechner, 1997). Based on its distribution, we estimated that N. v iridis would only survive in the warmer, southern parts of the continental United S tates, or Zones 9 - 10. One or more of its potential hosts occurs in these regions ( NRCS , 2007). Medium (2)

**Risk Element #2: Host Range** Nipaecoccus viridis is polyphagous with hosts across a few dozen plant families and genera, including: **Acanthaceae** ( Acanthus, Hygrophila , Peristrophe, Sanchezia), **Agavaceae** (

Agave), **Anacardiaceae** ( Mangifera ), **Annonaceae** ( Annona ), **Apiaceae** ( Apium ), **Apocynaceae** (Beaumontia, Holarrhena, Landolphia, Nerium, Odontadenia, Plumeria, Trachelospermum ), **Araceae** ( Arum, Cocos ), **Arecaceae** ( Cocos, Phoenix), **Asteraceae** ( Chrysanthemum **,** Helianthus **,** Parthenium, Tagetes ), **Bignoniaceae** ( Jacaranda, Spathodea, Tabebuia, Tecoma ), **Bombacaceae** ( Adansonia, Ceiba ), **Cactaceae** ( Opuntia ), **Caricaceae** ( Carica ), **Casuarinaceae** ( Casuarina ), **Clusiaceae** ( Calophyllum ), **Cucurbitaceae** ( Cucumis, Luffa ), **Ebenaceae** ( Diospyros ), **Euphorbiaceae** (

Acalypha, Breynia, Emblica, Chamaesyce, Glochidion, Jatropha, Manihot, Phyllanthus ), **Fabaceae** ( Abrus, Acacia, Albizia, Arachis, Caesalpinia, Cajanus, Ceratonia, Cicer, Dalbergia, Desmodium, Erythrina, Glycine, Intsia, Leucaena, Phaseolus , Pithecellobium, Psophocarpus, Tamarindus, Tephrosia ), **Flacourtiaceae** (

Flacourtia ), **Geraniaceae** ( Pelargonium ), **Lamiaceae** ( Leucas, Ocimum, Salvia ), **Lauraceae** (Persea ), **Liliaceae** ( Asparagus ), **Malvaceae** ( Abelmoschus, Alcea, Gossypium, Hibiscus, Sida, Thespesia ), **Meliaceae** (Khaya, Melia, Swietenia ), **Moraceae** (

Artocarpus, Ficus, Morus, Streblus ), **Musaceae** ( Musa ), **Myrtaceae** (

Eugenia, Myrthus, Psidium ), **Oleaceae** ( Ligustrum, Nyctanthes ), **Oxalidaceae** (

Averrhoa ), **Poaceae** (

Bambusa, Cynodon ), **Po rtulacaceae**  ( Portulaca ), **Proteaceae** ( Grevillea, Macadamia ), **Punicaceae** ( Punica ), **Rhamnaceae** (Ziziphus ), **Rosaceae** (

Eriobotrya, Pyrus, Rosa ), **Rubiaceae** ( Coffea, Ixora ), **Rutaceae** ( Citrus, Feronia, Glycosmis, Murraya ), **Sapindaceae** ( Gelonium, Nephelium, Sapindus ), High (3)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 54 **Nipaecoccus viridis (Newstead) (Homoptera: Pseudococcidae)**  **Risk r**  **ating s**  **Solanaceae** (

Datura, Solanum ), **Sterculiaceae** ( Melochia, Pterospermum ) , **Tamaricaceae** ( Tamarix ), **Theace ae**  ( Camellia ), **Tiliaceae** ( Corchorus ), **Verbenaceae** ( Clerodendron, Tectona, Verbena, Vitex), **Vitaceae** (Serianthus, Vitis ) (Ben
- Dov et al
. , 2007; CABI, 2007; Keanzlin, 1913; van den Berg, 1995). **Risk Element #3: Dispersal Potential** F ecundity ranges from 90 to 138 eggs per female, and up to three generations per year occur (CABI , 2007). Lo cal dispersal is accomplished by crawlers, which often settle in protected areas ( i. e. , under the sepals of fruitlets) , which predisposes them to dis semination on exported plants or plant products (CABI , 2007). High (3)
**Risk Element #4: Economic Impact** Nipaecoccus viridis attacks many crops that are important to the conterminous United Sates. This insect causes bulbous outgrowths on y oung twigs, which under heavy infestations stunt the growth of y oung trees (CABI, 2007). Heavy infestations on fruit lead to discoloration, disfiguration and fruit
- drop. Copious quantities of honey dew may contaminate fruit and other plant parts, which can serve as a medium for the growth of sooty molds (CABI , 2007). This mealy bug was responsible for losses of up to 5 percent in vineyards in I

ndia (CABI , 2007). Lo sses in citrus orchards

—for example, about 50 percent or more of the navel orange crop in South Africa

—are due primarily to fruit
- drop caused by large infestations (CABI , 2007). High (3)

**Risk Element #5: Environmental Impact** This pest poses a potential threat to F ederal Threatened and Endangered plants. Within the continental United States, these include Chamaesyce deltoidea ssp. deltoidea (FL), C
. garberi (FL), C
. hooveri (CA), Helianthus paradoxus (NM, TX), H
. schweinitzii (NC, SC), Manihot walkerae (TX), Opuntia treleasei (CA), Verbena californica (CA), and Ziziphus celata (FL) (USFWS, 2007). Because of its potential economic impacts (above), t he establishment of N

. viridis in the continental United States is likely to stimulate chemical and/or biological control programs (CABI, 2007; NASS , 2007). High (3)

**Planococcus angkorensis**  **( Takahashi** **) (Pseudococcidae)**  **Risk r**  **ating s**  **Risk Element #1: Climate -**  **Host Interaction** Planococcus angkorensis occurs in India, Indonesia, Cambodia, Malay sia, Philippines, Taiwan, Thailand, Vietnam, and China (Ben
- Dov et al
. , 2007). This broad region corresponds to Plant Hardiness Zones 9
- 11 (Widrlechner, 1997). Thus, w e estimate that P. angkorensis could survive in Zones 9 and 10 in the continental United States (USDA, 1990). One or more of its potential hosts occurs in these zones (NRCS , 2007). Medium (2)

**Risk Element #2: Host Range** Planococcus angkorensis is poly phagous. Known hosts include: **Aceraceae** ( Acer) **Anacardiaceae** (

Rhus ), **Araceae** ( Colocasia ), **Buddlejaceae** ( Buddleja ), **Daphniphyllaceae** (

Daphniphyllum ), **Dioscoreaceae** ( Dioscorea ), **Euphorbiaceae** ( Codae um , Euphorbia ), **Fabaceae** ( Pueraria ), **Loganiaceae** (Strychnos ), **Meliaceae** ( Lansium ), **Moraceae** (Ficus , Morus ), **Myrsinaceae** ( Myrsine ), **Myrtaceae** (

Eugenia , Psidium ), **Piperaceae** (

Piper), **Punicaceae** (

Punica ), **Rubiaceae** ( Coffea , Neonaucklea ), **Sapindaceae** (

Litchi), and **Ur ticaceae**  ( Boehmeria , Pipturus ) (Ben
- Dov et al
. , 2007). High (3)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 55 **Planococcus angkorensis**  **( Takahashi** **) (Pseudococcidae)**  **Risk r**  **ating s**  **Risk Element #3: Dispersal Potential** We found very

little published information on this pest , including no information on reproductive and dispersal potential. Therefore, w e assumed that the biology of P. angkorensis wa s similar to that of its congener P. minor . Thus, P. angkorensis probably is parthenogenetic, produces several hundred eggs per female, and has several generations per year , which gives it high reproductive potential. Its dispersal potential is probably also high , because scale insect crawlers are often transported over long distances on wind or animals (Greathead, 1990, 1997). Furthermore, scales can move great distances on infested commodities (e.g., P. minor, PestID , 2007; Sugimoto, 1994). High (3)

**Risk Element #4: Economic Impact** In our review , we did not find any information on the biological and economic impacts of this species. S ome mealybugs are important pests with significant economic impacts (e.g., P. minor ; Cox, 1989; Reddy et al
. , 1997), but Miller et al
. (2002) do not list P. angkorensis in their review of invasive mealy bugs that are a threat to the United States. De spite its exposure to a wide range of habitats and potential hosts (above) , P. angkorensis has not shown any

evidence to date of being an important economic pest. Although uncertain, we judged from this review that P. angkorensis is likely not an economically important pest. Medium (2)

**Risk Element #5: Environmental Impact** Based on the host range reported above, P. angkorensis may affect the following F ederal Threatened and Endangered (T&E) species that are congeners of current hosts: Euphorbia telephioides (FL) and Rhus michauxii (GA, NC, SC, VA) (USFWS, 2007). Because P. a ngkorensis is most likely not an economically important pest ( above ), its introduction to the United S tates will most likely not result in the initiation of control programs. Should it become economically

important, control measures already used on Planoco ccus citri in the United States (CABI , 2007) may likely be sufficient. Medium (2)

**Planococcus lilacinus (Cock) (Pseudococcidae)**  **Risk r**  **ating s**  **Risk Element #1: Climate -**  **Host Interaction** Planococcus lilacinus is reported throughout southern Asia (Andaman Is lands, Bangladesh, Bhutan, Brunei, Cambodia, I ndia, I ndonesia, Japan, Laos, Malaysia, Maldives, Myanmar, Ry

uky u Islands, Sri Lanka, Thailand, Taiwan, Vietnam and Yemen), islands o f the South Pacific (Cocos I slands, I ndonesia, Northern Marianas, the Philippines, Papua New Guinea), Africa (Comoros, Kenya, Madagascar, Mauritius, Mozambique, Reunion, Rodriques I sland, and Sey chelles), Central America & the Caribbean (Dominican Republic, El Salvador, Haiti), and northern South America (Colombia, Guy ana) (Ben
- D ov et al
. , 2007). Based on this distribution, we think this species could establish in Plant Hardiness Zones 9
- 11 (Maxwell, 2007; Widrlechner, 1997). We estimate that P. lilacinus could surviv e in Zones 9 and 10 in the continental United States (USDA, 1990). One or more of its potential hosts occurs in these zones (NRCS , 2007). Medium (2)

**Risk Element #2: Host Range** Planococcus lilacinus is very poly phagous, having been recorded on hosts in many plant families and genera, including: **Anacardiaceae** ( Mangifera, Spondias ), **Agavaceae** ( Cordyline), **Annonaceae** ( Annona, Cananga, **Apiaceae** ( Apium ), **Apocynaceae** High (3)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 56 **Planococcus lilacinus (Cock) (Pseudococcidae)**  **Risk r**  **ating s**  ( Carissa ), **Arecaceae** ( Cocos, Phoenix), **Asteraceae** ( Adenophyllum ), **Bombacaceae** ( Ceiba, Durio, Ochroma ) , **Boraginaceae** ( Cordia ) , **Brassicaceae** ( Brassica ), **Clusiaceae** ( Garcinia ), **Combretaceae** ( Quisqualis , Terminalia ), **Crypteroniaceae** ( Crypteronia ), **Cucurbitaceae** (Sechium ), **Dioscoreaceae** ( Dioscorea ), **Dipterocarpaceae** (

Dipterocarpus ), **Ebenaceae** (Diospyros ), **Ericaceae** ( Rhododendron ), **Eup horbiaceae**  ( Antidesma, Bridelia, Codiaeum, Croton, Euphorbia, Macaranga, Mallotus, Trewia ), **Fabaceae** ( Acacia, Albizia, Arachis, Bauhinia, Cajanus, Crotalaria, Derris, Erythrina, Gliricidia, Hymenaea, Mucuna, Prosopis, Saraca, Schizolobium, Sesbania, Tamarindus, Tephrosia, Vigna ), **Iridaceae** ( Gladiolus ), **Lecythidaceae** ( Couroupita ), **Lythraceae** ( Lagerstroemia ), **Malpighiaceae** ( Malpighia ), **Meliaceae** (

Lansium ), **Mimosaceae** (Prosopis ), **Moraceae** ( Artocarpus, Castilloa, Ficus, Streblus ), **Moringaceae** ( Moringa ), **Myrtaceae** (

Eugenia, Psidium, Syzigium ), **Orchidaceae** ( Dendrobium , Vanda ), **Oxalidaceae** ( Averrhoa ), **Pandanaceae** ( Pandanus ), **Poaceae** (

Bambusa ), **Punicaceae** (Punica ), **Rhamnaceae** ( Alphitonia, Ziziphus ), **Rhizophoraceae** ( Rhizophora ), **Rubiaceae** ( Coffea, Gardenia, Ne onauclea ), **Rutaceae** ( Citrus ), **Santalaceae** (Santalum ), **Sapindaceae** ( Aphania, Litchi, Nephelium ), **Sapotaceae** ( Manilkara ), **Sim aroubaceae**  ( Ailanthus ), **Solanaceae** ( Nicotiana, Solanum ), **Sterculiaceae** ( Heritiera, Theobroma ), **Verbenaceae** ( Callicarpa, Premna, Tectona ), **Vitaceae** ( Vitis ), and **Zingiberaceae** ( Elettaria ) (Ben
Dov et al
. , 2007; CABI, 2007; Krishnamoorthy and Mani, 1998; L oganathan and Suresh, 2001; Rostaman, 1997; Williams, 2004). **Risk Element #3: Dispersal Potential** Fecundity per female on Brassica oleracea is 55
- 152 eggs (Loganathan and Suresh, 2001) , and it has t

wo or three generations per year ( IP M DANIDA , 2007). The complete life cycle of P
. lilacinus takes about 40 days in Java (CABI, 2007). This insect is capabl e of limited dispersal on its own. Like other scales, it can passively disperse by

wind (Gullan and Kosztarab, 1997). Long - distance dispersal can be accomplished via human
- assisted movement of infested plant materials. Since 1985, P. lilacinus has been intercepted 1,920 times on fruit at U.S. ports
- of
- entry on a variety

of plant species (PestID , 2007). High (3)

**Risk Element #4: Economic Impact** Planococcus lilacinus is a major pest of tamarind and coffee in I ndia (Hill, 1983; Reddy et al
. , 1997), and damages a wide range of other economically important crops, including custard apple (

Annona reticulata ), coconut, citrus, grape, guava, and mango (CABI , 2007). Planococcus lilacinus feeds on R s, stems, leaves, inflorescences, and fruits, stunting plant growth, covering foliage with sooty mold, and lowering the quality of fruit (CABI, 2007). It feeds at the base of fruit on citrus , causing premature fruit drop (Williams, 2004). On coffee, it feeds on the R s , making the leaves chlorotic and eventually causing defoliation (Williams, 2004). On cauliflower, severe infestations lead to stunted plant growth, withering, and reduced flower size (Loganathan and Suresh, 2001). Due to honey dew production, sooty

mold may develop on plant surfaces, reducing photosy nthesis and market value of fruit (Williams, 2004). In I ndia, chemical and biological control programs are necessary to control this pest on a variety of crops (CABI , 2007; Mani and Krishnamoorthy, 2000; Reddy et al
. , 1997). High (3)

**Risk Element #5: Environmental Impact**  As a highly

poly phagous pest, P. lilacinus may attack F ederal Threatened and Endangered High (3)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 57 **Planococcus lilacinus (Cock) (Pseudococcidae)**  **Risk r**  **ating s**  (T&E) species that are congeners of current hosts, including Crotalaria avonensis (FL), Euphorbia telephioides (FL), Rhododendron chapmanii (FL), and Ziziphus celata (FL) (USFWS, 2007). Furthermore, because it threatens economically valuable crops such as avocado, citrus, and cucurbits (Miller et al
. , 2002), its introduction would likely stimulate chemical or biological control programs, bey ond those already

used . This is necessary

in India, for ex ample (CABI, 2007; Krishnamoorthy and Mani, 1998; Mani and Krishnamoorthy, 2000; Reddy et al
. , 1997). **Pseudococcus cryptus Hempel (Pseudococcidae)**  **Risk r**  **ating s**  **Risk Element #1: Climate**  **- Host Interaction**  Pseudococcus cryptus is primarily a subtropical to tropical species in several countries in southern Asia37 , Africa (Kenya, Mauritius, Tanzania), South America (Argentina, Brazil, Colombia, Paraguay), Central America (Costa Rica, El Salvador), the Caribbean (U.S. Virgin I slands), and Oceania (American Samoa, Federated States of Micronesia, Hawaii, Palau, Western Samoa) (Ben
- Dov et al
. , 2007) . It is also present in Japan and South Korea (Ben
- Dov et al
. , 2007). Other than the U.S. Virg in Islands, Hawaii, and American Samoa, it is not present in any other U.S. territory or protectorate. This distribution corresponds to Plant Hardiness Zones 8
- 13 (Maxwell, 2007; Widrlechner, 1997) , so w e estimate that it could survive in Zones 8 - 10 in the continental United States. One or more of its potential hosts occurs in these zones (NRCS , 2007). Medium (2)

**Risk Element #2: Host Range** Pseudococcus cryptus is a poly phagous pest that has been recorded on hosts in more than 30 families, including but not limited to **Anacardiaceae** ( Mangifera ) , **Annonaceae** ( Annona ) , **A pocynaceae**  ( Plumeria ), **Araceae** (

Amorphophallus, Aglaonema ), **Arecaceae** (

Areca , Cocos , Cyrtostachys , Elaeis, Metroxylon, Phoenix), **Asclepiadaceae** ( Finlaysonia ), **Asteraceae** ( Dahlia ), **Bromeliaceae** (

Ananas ), **Clusiaceae** ( Calophyllum, Garcinia ), **Dilleniaceae** (Dillenia, Tetracera ), **Euphorbiaceae** ( Croton , Hevea, Manihot), **Fabaceae** (

Bauhinia , Erythrina, Gliricidia, Glycine), **Flacourtiaceae** ( Ryparosa ), **Heliconiaceae** ( Heliconia ), **Lauraceae** ( Ocotea , Persea ), **Liliaceae** ( Crinum ), **Loganiaceae** ( Strychnos ), **Lythraceae** (Punica ), **Malvaceae** ( Hibiscus ), **Melastomataceae** (

Melastoma ), **Meliaceae** ( Lansium ), **Moraceae** (

Artocarpus ), **Moringaceae** (

Moringa ), **Musaceae** (

Musa ), **Myristicaceae** (

Myristica ), **Myrtaceae** ( Eugenia , Osbornia , Psidium ), **Oleaceae** ( Jasminum ), **Orchidaceae** ( Coelogyne, Dendrobium, Phalaenopsis, Raphioperdalum, Spathoglottis, Vanda ), **Pandanaceae** ( Pandanus ), **Passiflorace ae**  ( Passiflora ), **Piperaceae** ( Piper), **Poaceae** (

Bambusa ), **Rhizophoraceae** (

Rhizophora ), **Rubiaceae** ( Coffea , Gardenia , Ixora, Neonauclea ), **Rutaceae** ( Citrus ), **Selaginellaceae** (Selaginella ), **Verbenaceae** ( Avicennia ), and **Vitaceae** ( Vitis ), (Ben
- Dov et al
. , 2007; CABI, 2007; Santos, 1988). High (3)

**Risk Element #3: Dispersal Potential** Pseudococcus cryptus is a multivoltine species that can have up to six generations per y ear High (3)

37 Afghanistan, Andaman Islands, Bangladesh, Bhutan, British Indian Ocean Territory, Brunei, China (Hunan), India, Indonesia, Iran, Cambodia, Laos, Malaysia, Maldives, Nepal, Philippines, Singapore, Sri Lanka, Taiwan, Thailand, and VietnamPest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 58 **Pseudococcus cryptus Hempel (Pseudococcidae)**  **Risk r**  **ating s**  (Avidov and Harpaz, 1969). Females lay from 200 to 500 eggs, which then take 2
- 14 days to incubate. Although mealy bugs in general are somewhat limited in their dispersal ability, P. cryptus can be transported long distances by wind (Gullan and Kosztarab, 1997) or in shipments of fruit and other plant material (PestID , 2007). For example, after P. cryptus was first detected in a citrus grove in I srael, it rapidly spread to over 9,000 acres in just three years (Avidov and Harpaz, 1969). Since 1985, P
. cryptus has been intercepted over 52 0 times on plant materials at U.S. ports - of
- entry ; most of these interceptions were on fruit (PestID , 2007). **Risk Element #4: Economic Impact** Pseudococcus cryptus is a significant pest of citrus (Franco et al
. , 2004; Hill, 1983; Kwon et al
. , 2002). Itis reported as a “serious pe st” in Israel (Blumberg et al
. , 1999) and in Korea (Kwon et al
. , 2002). On citrus, P. cryptus can attack any part of the tree ( R to fruit), and produces enormous quantities of honeydew, on which sooty mold can form 5 - 8 mm thick layers (Avidov and Harpaz, 1969). InI srael, citrus infestations can be entirely covered by

sooty mold and waxen flakes , be coming so heavy that whole branches die, or leaves and fruit drop premature ly (Avidov and Harpaz, 1969). In south China, this species is considered onl y a minor pest, but it is widespread and similarly causes a large amount of sooty mold on the fruits and leaves (Hill, 1983). Pseudococcus cryptus also attacks coconut palms (Williams and Watson, 1988). In I srael, biological and chemical controls have maintained populations below economically damaging densities (Avidov and Harpaz, 1969; Blumberg et al
. , 1999), but with increase d production costs. I ntroduction of P. cryptus into the United States could negatively affect international and domestic trade for a number of commodities, but particularly for citrus, which is a multi
-billion dollar industry in the United States ( NASS , 2007). High (3)

**Risk Element #5: Environmental Impact** It is unknown if establishment of P
. cryptus would reduce community biodiversity

or disrupt ecological processes in natural areas . It may affect one F ederal Threatened and Endangered (T&E) species that is a congener of a current host: Manihot walkerae (TX) (USFWS, 2007). Because P. cryptus represents a major threat to U.S. citrus (Miller et al
. , 2002), its establishment in the United S tates would initiate a variety of control measures (e.g., as in I srael; Avidov and Harpaz, 1969; Blumberg et al
. , 1999) that may have indirect environmental impacts. High (3)

**Rhipiphorothrips cruentatus (Thysanoptera: Thripidae)**  **Risk r**  **ating s**  **Risk Element #1: Climate -**  **Host Interaction** Rhipiphorothriops cruentatus has been reported from Afghanistan, Bangladesh, the tropical/subtropical regions of China (including Taiwan), tropical/subtropical India, Myanmar, Oman, Pakistan, Sri L anka, and Thailand (CABI, 2007). Based on this distribution we estimate that R. cruentatus could become established in Plant Hardiness Zone s 8 - 10 in the United States (USDA, 1990). One or more of its potential hosts occurs in this zone (NRCS, 2007). Medium (2)

**Risk Element #2: Host Range** R. cruentatus has been reported on several hosts in several families including: High (3)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 59 **Rhipiphorothrips cruentatus (Thysanoptera: Thripidae)**  **Risk r**  **ating s**  **Anacardiaceae** (

Anacardium occidentale, Mangifera indica ), **Annonaceae** ( Annona squamosa ), **Combretaceae** ( Terminalia catappa ), **Myrtaceae** ( Psidium guajava, Syzygium spp.), **Lythraceae** ( Punica granatum ), **Rosaceae** ( Rosa rugosa ), and **Vitaceae** ( Vitis vinifera ) (CABI, 2007). **Risk Element #3: Dispersal Potential**  Thrips generally

have high biotic potential. Sexual reproduction is normal in R. cruentatus , but parthenogenesis is apparently common in India (CABI, 2007). Thrips are weak fl ye rs, but their fringed wings enable them to remain airborn long enough to travel easily

between neighboring fields , and frequently over greater distances (Lewis, 1997). In international trade, R. cruentatus could be carried on plant materials , but interceptions of this pest at U.S. ports of entry have been relatively

rare (PestID , 2007). Additionally, th e restricted distribution of this pest in Asia support s the idea that it has only moderate dispersal potential. Medium (2)

**Risk Element #4: Economic Impact** In India, R. cruentatus is one of the most important insect pests of grapevines (CABI, 2007) . Attacked leaves turn brown and fall prematurely , and the grape berries develop a corky surface. Also severely attacked in India are roses; again the leaves turn brown and fall premature ly . In Taiwan, wax apple and several other crops , including mango and guava , have been severely attacked , with resulting yi eld reductions and loss of market value (CABI, 2007). S ome thrips are vectors of plant pathogens but this species is not a known vect or of any

pathogen. Because R. cruentatus attacks grapes, which are an economically

important export crop in the United States, its presence in the United States, even as a temporary

adventive population, could lead to severe export restrictions of host co mmodities to markets outside of this pest’s known distribution. High (3)

**Risk Element #5: Environmental Impact** R. cruentatus is not known to attack any F ederal Threatened and Endangered (T&E) species or their congeners (USFWS, 2007). However, because R. cruentatus attacks grapes (above), its introduction to might initiate new control programs. Medium (2)

**Scirtothrips oligochaetus (Thysanoptera: Thripidae)**  **Risk r**  **ating s**  **Risk Element #1: Climate -**  **Host Interaction** Scirtothrips oligochaetus appears to be restricted geographically to India. Without more specific information, we assume it is found throughout the country , and estimate that S. oligochaetus could become established in Plant Hardiness Zones 9
- 10 in the United States (USDA, 1990). Medium (2)
**Risk Element #2: Host Range** Little is known about the host range of this pest. It probably attacks multiple genera in multiple families because it has been recorded on peanuts ( **Fabaceae:** Arachis hypogaea ) (CABI, 2007); cotton ( **Malvaceae:** Gossypium spp.) (Nair, 1975); and pomegranates ( **Lythraceae:** Punica granatum ) (Nair, 1975; Sheikh, 2006). High (3)
**Risk Element #3: Dispersal Potential** We found little information about the biology of this pest. Based on the biology of S. Medium (2)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 60 **Scirtothrips oligochaetus (Thysanoptera: Thripidae)**  **Risk r**  **ating s**  dorsalis, we assume that S. oligochaetus would have a high biotic potential. The potential of Scirtothrips spp. for natural spread appears to be limited (EPPO, 1997). Additionally, this species is limited to I ndia, which indicates limited dispersal potential. **Risk E lement #4: Economic Impact**  V ery little information on this pest ex ists in the scientific literature. It does not appear to be a major pest any

where in India. It

reportedly

attacks peanuts and cotton, however, which are important commodities in the United States . Thus, its presence in the United States, even as a temporary

adventive population, could lead to severe export restrictions of host commodities to markets outside of this pest’s known distribution. Medium (2)

**Risk Element #5: Environmental Impact** Compared to other thrips S. oligochaetus appears to have a very restricted host range. This relative oligophagy

suggests that this species is not a significant threat to Federal Threatened or Endangered (T&E) plants in the United States and no T&E species (listed in 50 CFR §17.12) are congeners of reported hosts. Medium (2)

**Tenuipalpus granati (Acari: Eriophyidae)**  **Risk r**  **ating s**  **Risk Element #1: Climate -**  **Host Interaction** Tenuipalpus granati has been reported in Eygpt, Iran, Greece, Georgia, Kazakhstan , and Azerbaijan (Jeppeson et al
. , 1975). Based on this distribution we estimate that T. granati could establish in Plant Hardiness Zones 7
- 10 in the United States (USDA, 1990). One or more of its potential hosts occurs in this zone ( NRCS , 2007). High (3) **Risk Element #2: Host Range** The host range of T. granati appears to be fairl y narrow: It is mainly a pest of grapevines ( **Vitaceae** : Vitis vinifera ) (CABI, 2007; Jeppeson et al
. , 1975), but has occasionall y been associated with pomegranates ( **Lythraceae** : Punica granatum ) (Jeppeson et. al, 1975). Medium (2) **Risk Element #3: Dispersal Potential** Relatively

little is known about the biology of false spider mites. Compared to other pests and other mites in particular, false spider mites have medium biotic po tential. Females may

live as long as two months, but they have much lower reproductive rates than spider mites, and fecundity rarel y reaches 50 offspring per female (Zhang, 2003). False spider mites are very slow moving mites. Their movement and dispersal has not been studied but long distance dispersal probabl y occurs passivel y through air currents or though the transportation of infected plants or other materials (Zhang, 2003). Medium (2)

**Risk Element #4: Economic Impact** T. granati is reported to be an occasional pest in vineyards in Egy pt. It also infests leaves, small branches, and sometimes fruit of pomegranate trees. Itdoes not appear, however, to be a significant pest anywhere in the world. In California , grape and pomegranate producers already control for a variety

of mite pests (Carroll et al
. , 2006, U.C. IPM , 2007) . Therefore, establishment of this pest would not likely lead to significant increases in costs of production. Low (1)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 61 **Tenuipalpus granati (Acari: Eriophyidae)**  **Risk r**  **ating s**  The United States does not export a significant amount of pomegranates, but does export a large amount of fresh grapes to the following countries: Australia, Canada, China (including Hong Kong), Indonesia, India, Mexico, Malaysia, Philippines, Trinidad and Tobago, Taiwan, and the UK ( FAS , 2007). Onl y India is known to have this pest, therefore its establishment in the grape producing regions of the United States may lead to loss or restriction of these important markets. **Risk Element #5: Environmental Impact** Compared to other mites T. granati appears to have a very restricted host range. This relative oligophagy

suggests that this species does not constitute a significant threat to threatened or endangered plants in the United States and there are no Federally Threatened or Endangered species (listed in 50 CFR §17.12) that are congeners of reported hosts. As mentioned above , some producers already control for a variety of mite pests (Carroll et al
. , 2006, U.C. IPM , 2007) , and therefore the establishment of this pest is unlikely

to lead to new chemical and biological control programs. Low (1)

**Tenuipalpus punicae (Acari: Eriophyidae)**  **Risk r**  **ating s**  **Risk Element #1: Climate -**  **Host Interaction** Tenuipalpus punicae has been reported from Eygpt, Iran, Iraq, Spain (Jeppeson et al
. , 1975; CABI, 2007), and India (Dhooria and Bhullar, 2003; Prasad, 1971). Based on this distribution we estimate that T. granati could become established in Plant Hardiness Zones 9 - 10 in the United States (USDA, 1990). One or more of its p otential hosts occurs in this zone (

NRCS , 2007). Medium (2)

**Risk Element #2: Host Range** T. punicae is only

known to attack pomegranate ( **Lythraceae** : Punica granatum ) (CABI, 2007; Jeppeson et al
. , 1975). Low (1) **Risk Element #3: Dispersal Potential** Relatively

little is known about the biology of false spider mites. Compared to other pests and other mites in particular, false spider mites have medium biotic potential. Females may

live as long as two months, but they have much lower reproductive rates than spider mites, and fecundity rarel y reaches 50 offspring per female (Zhang, 2003). False spider mites are very slow moving mites. Their movement and dispersal has not been studied but long distance dispersal probabl y occurs passivel y through air curr ents or though the transportation of infected plants or other materials (Zhang, 2003). Medium (2)

**Risk Element #4: Economic Impact** T. punicae infests leaves, small branches, and sometimes fruit of pomegranate trees , but i t does not appear to be a significant pest anywhere in the world. In California , pomegranate producers already control for a variety of mite pests (Carroll et al
. , 2006) , so the establishment of this pest would likely only minimal ly increase production costs . The United States does not export a significant amount of pomegranates, but it does export a large amount of fresh grapes to the following countries: Australia, Canada, Low (1)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 62 **Tenuipalpus punicae (Acari: Eriophyidae)**  **Risk r**  **ating s**  China (including Hong Kong), Indonesia, India, Mexico, Malay sia, Philippines, Trinidad and Tobago, Taiwan, and the UK. Only India is known to have this pest, therefore its establishment in the grape producing regions of the United States may lead to loss or restriction of these important markets. **Risk Element #5: Environmental Impact** Compared to other mites T. punicae appears to have a very restricted host range. This relative oligophagy

suggests that this species does not constitute a significant threat to threatened or endangered plants in the United States and there are no Federally Threatened or Endangered species (listed in 50 CFR §17.12) that are congeners of reported hosts. As mentioned above , some producers already control for a variety of mite pests (Carroll et al
. , 2006, U.C. IPM , 2007) , and therefor e the establishment of this pest is unlikely

to lead to new chemical and biological control programs. Low (1)

**Xanthomonas axonopodis pv.**  **punicae Risk**  **r ating**  **s Risk Element #1: Climate/Host Interaction**  D isease development of Xanthomonas axonopodis pv. punicae depends on humidity, temperature, and rainfall. The minimum average temperature for disease development is about 28 ° C. (Kumar et al, 2009) Rainfall seems to be required for disease development more than humidity , but the amount needed or timing of rainfall has not been quantified. Humidity of 50 to 60 percent seems to promote disease development (Kumar et al, 2009) . The hardiness zones that correspond to these conditions are approximately zones 10 and 11 which exist only in South Florida (USDA, 1990) . . Since the pathogen could survive in only

two hardiness zones, the risk associated with climate/host interaction is low. Low (1)

**Risk Element #2: Host Range**  The only known host of Xanthomonas axonopodis pv. punicae is pomegranates , Punica granatum (Kumar et al 2009) . For this reason, the risk associated with the pathogen is low. Low (1) **Risk Element #3: Dispersal Potential** We found no information about the natural dispersal of Xanthomonas axonopodis pv. punicae . The association of the disease with rainfall may indicate that the pathogen is spread by rain splash (Kumar et al, 2009) . Depending on the amount of precipitation and the strength of the wind, splash es can dispers e materials from a meter to a kilometer . Although the reproduction rate of the pathogen is likely to be high, the amount of functional inoculum is likely to be much less

—in other words, even large numbers of bacteria yi eld many fewer infective bacteria . Thus, we think the probability of a bacterium infecting a tree or fr uit is very small. Overall, we thought the pathogen has Medium potential for both rapid reproduction and dispersal under the right circumstances. Medium (2)

**Risk Element #4: Economic Impact**  The primary

description of X. axonopodis pv. punicae infection states that it infects “all above ground parts of the plant , ” including a black spot on fruit , and lowers production by damaging fruit (Kumar et al, 2009) . To date , X. axonopodis pv. punicae is only present in Medium (2)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 63 **Xanthomonas axonopodis pv.**  **punicae Risk**  **r ating**  **s** India (Kumar et al, 2009) . If it w ere introduced into the United S tates , other countries may enact quarantine restrictions on U.S. pomegranates to prevent its entry . For these reasons, we rated this element a s Medium. **Risk Element #5: Environmental Impact**  Since the only

known host of X

. axonopodis pv. punicae is pomegranates (Kumar et al, 2009) , it likely

has little ability

to damage other species. Thus, the environmental impact is low. Low (1)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 64 **Table 6.** Summary

of risk ratings for Consequences of Introduction **Pest Risk**  **elements Cumulative**  **Risk Rating Climate/**  **host int er**  **** **action Host** 

**range Dispersal** 

**potential Economic** 

**impact Envir.** 

**impact** Bactrocera carambolae Low (1) High (3) High (3) High (3) High (3) High (13) Bactrocera dorsalis Med (2) High (3) High (3) High (3) High (3) High (14) Bactrocera zonata Med (2) High (3) High (3) High (3) High (3) High (14) Ceroplastes rubens Med (2) High (3) High (3) Med (2) High (3) High (13) Ceroplastes rusci Med (2) High (3) High (3) Med (2) High (3) High (13) Conogethes punctiferalis High (3) High (3) High (3) High (3) High (3) High (15) Cryptoblabes gnidiella High (3) High (3) High (3) High (3) High (3) High (15) Deudorix epijarbas High (3) High (3) High (3) Med (2) Med (2) High (13) Deudorix isocrates Med (2) High (3) High (3) High (3) High (3) High (14) Dichocrocis megillalis Low (1) High (3) Med (2) Med (2) Med (2) Med (11) Drosicha dalbergiae Med (2) High (3) High (3) High (3) High (3) High (14 ) Dysmicoccus neobrevipes Med (2) High (3) High (3) Med (2) Med High (13) Euzophera bigella Med (2) High (3) High (3) High (3) High (3) High (14) Icerya aegyptiaca Low (1) High (3) High (3) Med (2) Med (2) Med (11 ) Icerya seychellarum Med (2) High (3) High (3) High (3) High (3) High (14) Maconellicoccus hirsutus Med (2) High (3) High (3) Med (2) High (3) High (13) Nipaecoccus viridis Med (2) High (3) High (3) High (3) High (3) High (1 4 ) Planococcus angkorensis Med (2) High (3) High (3) Med (2) Med (2) Med (12 ) Planococcus lilacinus Med (2) High (3) High (3) High (3) High (3) High (14 ) Pseudococcus cryptus Med (2) High (3) Med (2) High (3) High (3) High (14 ) Rhipiphorothrips cruentatus Med (2) High (3) Med (2) High (3) Med (2) Med (12 ) Scirtothrips oligochaetus Med (2) High (3) Med (2) Med (2) Med (2) Med (11) Tenuipalpus granati High (3) Med (2) Med (2) Low (1) Low (1) Med (9) Tenuipalpus punicae Med (2) Low (1) Med (2) Low (1) Low (1) Low (7) Xanthomonas axono
podis pv . puniceae Low (1) Low (1) Med (2) Med (2) Low (1) Low (7) **2.7. Likelihood of Introduction** Likelihood of introduction is a function of both the quantity of the commodity

imported annuall y and pest opportunity , which consists of five criteria that consider the potential for pest survival along the pathway

(USDA, 2000). The values determined for the Likelihood of Introduction for eac h pest are summarized in Table 7 . **2.7. 1. Quantity of commodity imported annually** Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 65 The rating for th is subelement is based on the amount reported b y th e exporter, and is converted into standard units of 40
- foot
- long shipping containers . 38 In dia expects initially to ship about 1500 tons of pomegranates per ye ar to the United States , which equals about 50 shipping containers. Therefore, we ranked this element Medium (2) for all pests. **2.7. 2. Survive post** **- harvest treatment**  Standard post - harvest treatments f or pomegranates include culling , washing , and cold storage (Prat and Botti, 2002). Larvae of the fruit flies ( Bactrocera carambolae, B. dorsalis, B. zonata ), are internal feeders and are highl y likel y to surviv e these post - harvest treatments, especiall y if the damage is not obvious. S urface p uncture marks from entry of the female’s ovipositor may be visible but recognizing fruit fly
- infested fruit is difficult , especiall y if the flies enter through the deep caly x (White and Elson
- Harris, 1992) . We rated these pests as H igh risk to surviv e post harvest treatment s . A number of the pests are small external feeders , as follows: the scales Ceroplastes spp. , Drosicha dalbergiae, Dysmicoccus neobrevipes, Icerya spp. , Maconellicoccus hirsutus, Nipaecoccus viridis, Planococcus angkorensis, P. lilacinus, and Psedococcus cryptus; the thrips Rhipiphorothrips cruentatus, Scirtothrips
. oligochaetus; and the mites Tenuipalpus granati and T. punicae . These pests are less likely to survi ve post - harvest treatment s if they are on the surface of the fruit. However, the deep caly x of pomegranates offers con s iderable shelter for all stages of these arthropods . Such cry ptic behavior could protect them well from detection or removal. Therefore, we gave these pests a H igh risk rating for this subelement . Conogethes punctiferalis, Cryptoblabes gnidiella, the three Deudorix spp., Dichocrocis megillalis, and Euzophera bigella may be present as eggs, on or in the fruit (Hel y et al
. , 1982) or as larvae (Hel y et al
. , 1982; Whittle, 1984) . These pests also could survive treatment, particularl y if sheltered at the cal yxor stem ends. Fruit newly invaded by

larvae or that have larvae which entered b y the cal yx , might go undetected. The damage caused by larvae boring into fruit and the frass (excrement) they produce are likel y to be more evident than s ymptoms caused by fruit fl ies (above). Consequently , these moths are more likely to be detected and culled, and we gave them M edium ratings . Washing and culling could wash Xanthomonas bacteria off the surface of the fruit but would not affect population s liv ing just below the surface of the fruit. We gave a risk rating of High for this pathogen to survive post harvest treatment. **2.7. 3. Survive Shipment**  Insect survival at low temperatures is influenced by the duration of exposure ( Chapman, 1998 ). Low temperatures above freezing are not immediately fatal for most insect s; some remain alive for considerable periods even at temperatures around 0°C. As temperatures drop, physiological adjustments , or acclimation , may lengthen the tolerance of insects to chilling ( Salt, 1961 ). Pomegranates are typically shipped at 5oC (41o F) (McGregor, 1989). At this temperature, we expect that all but the most cold sensitive arthropods will survive. Consequently , we rated all

38 The conversión factor is 60,000 lbs (30 tons) per 40
- foot long refrigerated shipping container.Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 66 arthropods High for this element , except Bactrocera carambolae, Dichocrocis megillalis, and Icerya aegyptiaca, which we rated M edium because of their cold sensitivity . The only research on the survivability of X. axonopodis pv. punicae observed that the bacteria survived at a 100 percent recovery rate from fruit and leaves after 120 day s at room temperature (Upasana and Verma, 2002) . This finding would suggest that survival of the bacteria is highl y likely by almost any conveyance. Thus, the chance that bacteria would survive shipment is High . **2.7. 4. Not detected at a port of entry**  The likelihood of pests not be ing detected at a port
- of
- entry depends on pest size, mobility, and degree of concealment. If an infestation occurred relatively recently , internal feeders have a high probability

of escaping detection at a port
- of
- entry , unless fruit is cut open. Consequently , we rated all internal pests High for this risk element. By comparison, the external feeders (see list above) are much more likel y t o be detect ed b y visual inspection. They

may still, however, be concealed beneath the cal yx or in packing materials . They

may also be present in the egg stage . Thus , we rated these species M edium for this risk element. In general pathogens are less likely to be correctly identified at the port level, especiall y if no fruiting body or unambiguous structure associated with the pathogen exists. Detection at port of entry for X. axonopodis pv. punicae is uncertain, and depend s upon the number and size of black spots on fruit (Upasana and Verma, 2002) . Thus, a severe infection could be obvious , while o thers could easil y pass unobserved. Thus, for X. axonopodis pv. punicae we rated this element High . **2.7. 5. Imported or moved subsequently to an area with an environment suitable for**  **survival** Pomegranates from India are likel y to be sold in all 4 9 of the state s within the continental United States . We assume that demand for fruit is proportional to the size of the consumer population in potential markets and that any infestations would be distributed equall y am ong consignments. Based on the latest census data (U . S . C ensus B ureau , 200 6 ) , 4.4 percent of the U.S. population resides within Plant Hardiness Z one 10 (PERAL, 2008) . Therefore, because the region encompassing zone 10 comprises only a small percentage of the likel y markets for Indian pomegranates , we gave Bactrocera carambolae, Dichocrocis megillalis, and Icerya aegyptiaca a L ow risk rating of being moved to habitat suitable for survival. The following pests can establish in Zone 8 and above: Bactrocera dorsalis, B. zonata, Ceroplastes spp. , Deudorix spp ., Drosicha dalbergiae, Dysmicoccus neobrevipes, Euzophera bigella, Icerya seychellarum, Maconellicoccus hirsutus, Nipaecoccus viridis, Planococcus spp., Pseudococcus cryptus, Rhipiphorothrips cruentatus, Scirtothrips oligochaetus, and Tenuipalpus punicae . Those zones in the continental United States have 41.6 percent of the U.S. population (PERAL , 2008). Consequently , we gave these pests a Medium risk rating for this element.Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 67 The remaining arthropods could surviv e in Zones 6
- 10 in the continental United States (above) . Th ese Z ones contain almost 87 percent of the U.S. population (PERAL , 2008) . Moreover, this region includes several of the most populous states ( e.g ., Illinois, New York, Ohio, Pennsylvania) to which a significant percentage of imports likel y would be diverted. Thus, we gave risk ratings of High to these remaining athropods for this subelement . P omegranate is the onl y known host of X. axonopodis pv. punicae , and they are onl y grown commerciall y in C entral California , which is Z one s 8 - 9. Based on the existing data, the disease might survive in such a climate but its spread would be minimal. Inaddition, C entral California is geographicall y separated from the larger urban areas where the fruit will primarily be marketed. Given these factors , we gave a risk rating of Lo w to this pathogen . **2.7. 6. Come into contact with host material suitable for reproduction**  Even if the climate for a given pest is conducive for survival , suitable host material must be available in order for the pest to live and reproduce. Pests such as scales and mites have limited natural dispersal ability and tend to remain with or near their host plants . Thus, they are relativel y unlikely

to contact hosts because infested fruit would have to be disposed in areas where th e larvae could survive (e.g., parks, crop fields, gardens, orchards, roadsides ) . Pests in fruit that are disposed of in trash bins, compactors, or garbage disposals

—even if uneaten

— would not be able to survive, and thus pose no risk. Therefore, we rated these pests L ow for this risk sub element. P ests that have relativel y narrow host ranges, or those that feed onl y on tropical and subtropical species with restricted distributions in the continental United States, are also unlikely to contact suitable hos t material. Therefore, we rated the fruit fly Bactrocera carambolae and the moth Dichocrocis megillalis , Low for this sub element, even though they are highly mobile species. Bactrocera are also highly mobile (above), but its hosts are available throughout the continental United States . Therefore, we rated the risk that Bactrocera dorsalis and B. zonata could contact host material as H igh . Similarly , the moths anal yz ed are capable of long distance flight , and are not monophagic (above) . Therefore, we also gave High risk ratings to Conogethes punctiferalis, Cryptoblabes gnidiella, Deudorix spp., and Euzophera bigella . The whitefl y Aleurodicus disperses and the thrips also fl y, although not as well, and may be aided in dispersal b y wind and human activities ( above ) . In comparison to the fruit flies and moths, then, we gave these pests a M edium risk rating for this subelement . The pathogen must contact pomegranate in order to establish (above). P omegranate s are mainly grown in three counties in the Central Valley in California : Fresno, Kern, and Tulare. These counties have onl y 5 percent of California’s population, and less than 0.6 percent of the national population (U.S. Census Bureau, 2006). Assuming again that demand will be proportional to population size, the probability that an infec ted pomegranate from India would end up in these counties in California is very small. In addition, the acreage of pomegranates in those counties is tiny compared to other crops, so any infected produce is much more likel y t o contact hosts other than pomegranate. For those reasons, we gave a risk rating of Low for this pathogen.Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 68 **Table 7.** Risk Rating s for Likelihood of Introduction . The risk rating for “Quantity imported annually” was Medium (2) for all pests. **Pest Risk elements**  **Cumulative Survive**  **p ost** **** **h arvest**  **t reatment**  **Survive**

**ship**   ** ment** **Not**

**d etected**  **at p**  **ort of e**  **ntry Moved** 

**to s**  **uitable h**  **abitat Contact** 

**with h**  **ost m**  **aterial r** **isk r**  **ating**
Bactrocera carambolae High (3) Med (2) High (3) Low (1) Low (1) Med (12) B. dorsalis High (3) High (3) High (3) Med (2) High (3) High (16) B
. zonata High (3) High (3) High (3) Med (2) High (3) High (16) Ceroplastes rubens High (3) High (3) Med (2) Med (2) Low (1) Med (13) C
. rusci High (3) High (3) Med (2) Med (2) Low (1) Med (13) Conogethes punctiferalis Med (2) High (3) High (3) High (3) High (3) High (16) Cryptoblabes gnidiella Med (2) High (3) High (3) High (3) High (3) High (16) Deudorix epijarbas Med (2) High (3) High (3) Med (2) High (3) High (15) D
. isocrates Med (2) High (3) High (3) Med (2) High (3) High (15) Dichocrocis megillalis Med (2) Med (2) High (3) Low (1) Low (1) Med (11) Drosicha dalbergiae High (3) High (3) Med (2) Med (2) Low (1) Med (13) Dysmicoccus neobrevipes High (3) High (3) Med (2) Med (2) Low (1) Med (13) Euzophera bigella Med (2) High (3) High (3) Med (2) High (3) High (15) Icerya aegyptiaca High (3) Med (2) Med (2) Low (1) Low (1) Med (11) I
. seychellarum High (3) High (3) Med (2) Med (2) Low (1) Med (13) Maconellicoccus hirsutus High (3) High (3) Med (2) Med (2) Low (1) Med (13) Nipaecoccus viridis High (3) High (3) Med (2) Med (2) Low (1) Med (13) Planococcus angkorensis High (3) High (3) Med (2) Med (2) Low (1) Med (13) P
. lilacinus High (3) High (3) Med (2) Med (2) Low (1) Med (13) Pseudococcus cryptus High (3) High (3) Med (2) Med (2) Low (1) Med (13) Rhipiphorothrips cruentatus High (3) High (3) Med (2) Med (2) Med (2) Med (14) S cirtothrips oligochaetus High (3) High (3) Med (2) Med (2) Med (2) Med (14) Tenuipalpus granati High (3) High (3) Med (2) High (3) Low (1) Med (14) T
. punicae High (3) High (3) Med (2) Med (2) Low (1) Med (13) Xanthomonas axonopodis pv. punicae High (3) High (3) High (3) Low (1) Low (1) Med (13)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 69 

### 3 **. Conclusion**  We summ ed the values for the Consequences of Introduction and the Likelihood of introduction to give Pest Risk Potentials (USDA, 2000) (Table 8). Th ese estimate the overall risk associated with the importation of pomegranates from India for pe sts that may be associated with the fruit. Pests with a Pest Risk Potential value of Low do not require specific mitigation measures, whereas a value within the Medium range indicates that specific phy tosanitary measures may

be necessary . The PPQ Guidelines state that a High Pest Risk Potential means that specific phy tosanitary measures are strongl y recommended, and that port
- of
- entry inspection is not considered sufficient to provide phy tosanitary security . The choice of appropriate measur es to mitigate risk is undertaken as part of Risk Management and is not addressed in this document.Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 70 **Table 8.** Pest Risk Potential s for pomegranate from India **Pest Cumulative Risk Ratings**  **Pest Risk Potential**  **Consequences of Introduction**  **Likelihood of**

**Introduction** Conogethes punctiferalis High (15) High (16) High (31) Cryptoblabes gnidiella High (15) High (16) High (31) Bactrocera dorsalis High (14) High (16) High (30) Bactrocera zonata High (14) High (16) High (30) Deudorix isocrates High (14) High (15) High (29) Euzophera bigella High (14) High (15) High (29) Deudorix epijarbas High (13) High (15) High (28) Drosicha dalbergiae High (14) Medium (13) High (27) Icerya seychellarum High (14) Medium (13) High (27) Nipaecoccus viridis High (14) Medium (13) High (27) Planococcus lilacinus High (14) Medium (13) High (27) Pseudococcus cryptus High (14) Medium (13) High (27) Ceroplastes rubens High (13) Medium (13) Medium (26) Ceroplastes rusci High (13) Medium (13) Medium (26) Dysmicoccus neobrevipes High (13) Medium (13) Medium (26) Maconellicoccus hirsutus High (13) Medium (13) Medium (26) Rhipiphorothrips cruentatus Medium (12) Medium (14) Medium (26) Bactrocera carambolae High (13) Medium (12) Medium (25) Planococcus angkorensis Medium (12) Medium (13) Medium (25) Scirtothrips oligochaetus Medium (11) Medium (14) Medium (25) Tenuipalpus granati Medium (9) Medium (14) Medium (23) Dichocrocis megillalis Medium (11) Medium (11) Medium (22) Icerya aegyptiaca Medium (11) Medium (11) Medium (22) Tenuipalpus punicae Low (7) Medium (13) Medium (20) Xanthomonas axonopodis pv. punicae Low (7) Medium (13) Medium (20) 

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- 10. Williams, D. J. 2004. Mealy bugs of Southern Asia. The Natural History Museum & Southdene SDN. BHD., Kuala Lumpur, Malay sia. 896 pp. Williams, D. J., and G. W. Watson. 1988. The scale insects of the tropical South Pacific region: Part 2: The meal ybugs (Pseudococcidae). CAB International Institute of Entomology , London, UK. 259 pp. Wisam, O. and A. Mazen. 2002. Bionomics and control of pomegranate butterfl y Virachola ( Deudorix) livia (Klug) (Lepidoptera: Lycanidae) in Northern Jordan. Dirasat. Agricultural Sciences, 29 (1): 1 - 12 WSSA. 2006. Weed List. Weed Science Society of America (WSSA). Last accessed November 28, 2006, http://www.wssa.net/weednames/common.asp . Wy nter- Bl yth, M. A. 1957. Butterflies of the Indian Region. The Bombay Natural History Society , Bombay , India. 523 pp. Zaka
- ur - Rab, M. 1980. The cornelian, Deudorix epijarbas Moore (Lepidoptera: Lycaenidae) as a serious pest of pomegranate fruits in Kashmir. Journal of Entomological Research 4:233
- 235. Zhang, B. C. 1994. Index of economicall y important L epidoptera. CAB International, Wallingfo rd, UK. 599 pp. Zhang, Z. - Q. 2003. Mites of Greenhouses: Id entification, Biology and Control. CABIPublishing, Wallingford, UK. 244 pp. Zlotina, M., M.K. Hennessey , R. Sequeira, and T.W. Culliney . 2007. Phy tosanitary Risks Associated with Armored Scales in Commercial Shipments of Fruit for Consumption to the United States . USDA APHI S Center for Plant Health Science and Technology , Raleigh, NC. 

### 5 **. Authors and Reviewers Authors:**  A. D. Neeley , Entomologist, PestID PPQ / CPHST
- PERAL A.L . Koop, Botanist, PestID PPQ / CPHST
- PERAL J. S. Rogers, Plant Pathologist, PestID PPQ / CPHST
- PERAL **Reviewer s**  **:** K.R. L akin, Entomologist , PestID PPQ / CPHST
- PERAL S.C. Redlin , Plant Pathologist, PestID P PQ / CPHST
- PERALPest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 89 

### Appendix: Risk Mitigation Options for Importation of Fresh Pomegranates **( Punica granatum**  **) Fruit from India into the Continental United States including Alask** **a 1**  **. Introduction** This section outlines possible phy tosanitary meas ures that should be considered in order to prevent the introduction of the q uarantine pests that likely associated with fresh pomegranate fruit from India . We identified the following pests with **Hi gh**  unmitigated pest risk potential: Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) Bactrocera zonata (Saunders) (Diptera: Tephritidae) Conogethes punctiferalis (Guenée) (Lepidoptera: Crambidae) Cryptoblabes gnidiella Millière (Lepidoptera: Pyralidae) Deudorix epijarbas (Moore) (Lepidoptera: Lycaenidae) Deu dorix isocrates Fabricius (Lepidoptera: Lycaenidae) Drosicha dalbergiae (Green) (Hemiptera: Margarodidae) Euzophera bigella Zeller (Lepidoptera: P yr alidae) Icerya seychellarum Westwood (Hemiptera: Margarodidae) Nipaecoccus viridis (Newstead) (Hemiptera: Pseudococcidae) Planococcus lilacinus (Cockerell) (Hemiptera: Pseudococcidae) Pseudococcus cryptus Hempel (Hemiptera: Pseudococcidae) We identified the following pests with **M edium**  risk potential: Bactrocera carambolae (Drew & Hancock) (Diptera: Tephritidae) Ceroplastes rubens (Maskell) (Hemiptera: Coccidae) Ceroplastes rusci (Linnaeus) (Hemiptera : Coccidae) Dichocrocis megillalis Walker (Lepidoptera: Pyralidae) Dysmicoccus neobrevipes Beardsley (Hemiptera: Pseudococcidae) Icerya aegyptiaca (Douglas) (Hemiptera: Margarodidae) Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae) Planococcus angkorensis (Takahashi) (Hemiptera: Pseudococcidae) Rhipiphorothrips cruentatus Hood (Thy sanoptera: Thripidae) Scirtothrips oligochaetus Kary ny (Thys anopter a: Thripidae) Tenuipalpus granati Sayed (Acari: Tenuipalpidae) Tenuipalpus punicae Pritchard & Baker (Acari: Tenuipalpidae) Xanthomonas axonopodis pv. Punicae (Hingorani & Singh) (Xanthomonads)Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 90 **2 .**  **Risk Mitigation Options** Pest risk management is the decision
- making process of reduci ng the likelihood of introduction of a quarantine pest ( IPPC, 200 7: I SPM #s 2 and 11) . The appropriate level of protection for an importing country

can be achieved at times by the application of a single phy tosanitary measure, such as inspection or a quarantine treatment, or a combination of measures. If no single measure is likely to reduce pest risk to an acceptable level, then a combination of measures may be needed. The combination of specific phy tosanitary measures that provides overlapping or redundant safeguards is called a “s ys tems approach .” The Plant Protection Act (7 U.S.C. § 7701
- 7786, 2000) defines “ s ys tems a pproach” as “...a defined set of phyt osanitary procedures, at least two of which hav e an independent effect in mitigating pest risk associated with the movement of commodities. ” Similarly, the FAO Standard for Integrated Measures for Pest Risk Management (June 2002) proposed that a s ys tems a pproach be defined as, “The integration of different pest risk management measures, at least two of which act independently , and which cumulatively achieve the desired level of phy tosanitary protection” ( IPPC, 200 7: I SPM #14) . Sy stems approaches vary in complexity ; however, all require the integration of two or more measures that act independently of each other, with the cumulative effect achieving the desired level of phyt osanitary

protection ( IP PC, 200 7: I SPM #14) . Specific mitigations may be selected from a range of pre
- harvest and post
- harvest opti ons, and may include other safeguarding measures. Measures may be added or the strength of measures increased to compensate for uncertainty. At a minimum, for a measure to be considered for use in a s ys tems approach, it must be: 1) clearl y defined; 2) effi cacious; 3) officially required (mandated); and 4) subject to monitoring and control by the responsible national plant protection organization (IPPC, 2007: ISPM #14) . Plant Protection and Quarantine (PPQ) uses sy stems approaches for the importation of many commodities including Unshu oranges from Japan [ 7 CFR § 319.28 ] , tomatoes from Spain, France, Morocco, and Western Sahara [ 7 CFR § 319.56 28 ] , and peppers from Israel [ 7 CFR § 319.56 24 ] . These programs have been successful for many years. A sy stems approach to mitigate risks involved with pomegranate imports from In dia might combine a variety

of measures: 1) certification of pest free areas, pest free places of production, or areas of low pest prevalence for certain quarantine pests; 2) programs to control pests within fields ( e.g ., mechanical, chemical, cultural) 3) preclearance oversight by USDA - APHIS officials 4) packinghouse procedures to eliminate external pests and pathogens ( e.g ., washing, brushing, inspection of fruit) 5) quarantine treatments to disinfest fruit of internal and external pests and pathogens 6) consignments inspected and certified to be free of quarantine pests and pathogens 7) fruit traceable to origin, packing facility , grower, and field 8) consignments subject to sampling and inspection after arrival in the United States 9) limits on distribution and transit within the United StatesPest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 91 **2.1 .**  **Phytosanitary Measures Prior to Harvesting**  **2.1. 1**  **. Establishment of Pest** **- free Areas**  A pest free area (PFA) is defined as “ an area in which a specific pest does not occur as demonstrated b y scientific evidence and in which, where appropriate, this condition is being officiall y maintained” (IPPC, 200 7: I SPM #s 4 and 5 ). As a sole mitigation measure, the establishment of pest free areas or pest free places of production may be completely

effective in satisfying an importing country ’s appropriate level of phy tosanitary protection (IPPC, 2007: ISPM #s 4 and 10) . This option has been successful in practice, eliminating the need for pos t harvest commodity

treatments to achieve probit
- 9 - level security [e.g., TDOA, 2003]. Establishment and maintenance of pest free areas or production sites are to be in compliance with international standards [e.g., IPPC, 200 7: I SPM #s 4, 5, and 10 ; NAPPO, 2004]. These standards specify

the appropriate steps for establishment, maintenance, verification, changes in status, emergency action plan, reinstatement of status, documentation, and bilateral workplans for fruit fl y PFA. Trapping for all fruit flies ( Bactrocera carambolae, B. dorsalis, B. zonata ) should be conducted in production areas throughout the ye ar b y In dia’s Ministry of Agriculture, with appropriate lures and traps. In addition, routine inspection and fruit sampling should be carried out in the PFA. The capture of a single fruit fly (of any species) within the pest free area should lead to an immediate emergency action and may

result in the cancellation of export from the PFA. Pomegranates would not be exported until the source of the infestatio n is delimited, trap density is increased, pesticide sprays are applied or other measures are undertaken. Freedom from the Lepidopteran pests and the pathogens may be achieved through controlled and effective integrated pest management (IPM) programs. Area freedom should be verified b y conducting an official survey s. P heromones and attractants are available to monitor Conogethes punctiferalis, Cryptoblabes gnidiella, and Euzophera bigella (El
- Sayed, 2008) ; work on the isolation and identification of the p heromone s ys tem of Deudorix isocrates is apparentl y currentl y being conducted (IICT, 2005). In addition, black
- light traps have been used successfull y to monitor other Lepidopterans (e.g., Cameron et al
. , 2002; Cherry and Wilson, 2005; Ngollo et al
. , 2000). **2.1. 2**  **. Establishment of Areas of Low Pest Prevalence**  According to the IPPC (200 7: I SPM # 22 ), an area of low pest prevalence may comprise all of a country , part of a country, or parts of several countries, in which a particular pest species occurs at low population densities and which is or are subject to effective surveillance and control or eradication measures (IPPC, 2007: I SPM #10 ). Procedures for the establishment and maintenance of areas of low pest prevalence are to comply with international standards [e.g., IPPC, 200 7: I SPM #22 ; NAPPO, 2003]. For example, elements of an operational plan for establishment and maintenance of such areas might include a geographic description to delimit the area; specification of an upper limit to pest densities ; means to document and verify all necessary procedures and maintain records; specification of phy tosanitary procedures (e.g., survey , pest control); and movement controls to prevent pest entry or re - entry

into the area. The international standards recommend that the exporting country consult with the importing country in the earl y stages of implementation to ensure that importing country requirements are met. In particular, target or threshold population densities defining an area of low pest prevalence are toPest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 92 be established in consultation with the importing country. Any protocol for establishing and maintaining a pest free area or area of low pest prevalence is also to include a pest
- reporting procedure and emergency action plan to address target pest d etections in the pest free or low
- prevalence zones (IPPC, 200 7: I SPM #s 10 and 22 ; NAPPO, 2003, 2004). Combined with inspection, effective IPM programs achieving low pest prevalence may be a possible mitigation option for the Lepidopteran, scales, thrips and mites but should be verified by conducting an official survey or lot freedom based on inspection of shipping lot. **2.1. 3**  **. Control Program**  Cultural, chemical, biological, or mechanical controls (e.g., grove sanitation, biocontrol, pre
harvest applicati on of pesticides/fungicides, traps, fruit bagging) may be used to suppress or eliminate pests from groves or prevent fruit infestation/infection. This is not a stand
- alone mitigation option, but should be used in combination with other measures as part of a s ys tems approach. **2.1. 4**  **. Phytosanitary Certification Inspections**  Sampling and inspection of pomegranate should occur periodicall y during the growing season and after harvest for quality control and as phy

tosanitary precautionary

measures. Production areas also should be subject to scheduled audits and periodic, unannoun ced, inspections by certified inspectors from PPQ and India’s Ministry of Agriculture; these inspections would insure that production areas meet stipulated requirements for the issuance of a phyt osanitary certificate required for each consignment. This mea sure is useful for detecting pests present during the growing season that may be more difficult to detect post
- harvest. Detection methods need to be combined with other measures to ensure the absence of pests of concern. Statistical procedures are availabl e to verify , to a specified confidence level, the pest free status of an area, given negative survey

or trapping results ( Barclay and Hargrove, 2005). Results of survey s must be negative for fruit fl y l arvae, Lepidopteran, and external pests. Each shipment of pomegranate is to be accompanied b y a phy tosanitary certificate of inspection issued by the NPPO of India. Additionally , production areas should be subject to periodic, unannounced inspections by certified inspectors from PPQ and India’s Ministry of Agriculture to ensure that they meet stipulated requirements. **2.2 .**  **Mitigation Options Post -**  **harvest and Prior to Shipping 2.2.**  **1 .**  **Post -**  **harvest Safeguards and Packinghouse Procedures** According to a risk assessment produced b y the Government of India’s Ministry of Agriculture, pomegranates are processed at a packinghouse immediately after harvest. At the packinghouse, the fruits are cleaned and washed with potable water sanitized with sodium hyphochlorite (200 ppm) and then given a fungicidal treatment to protect against post
- harvest storage fungal rots. They then undergo sorting and grading. Pomegranates for export are packed in boxes; clean paper shredding is kept below, in between, and on top of fruit to prevent bruising during transport and are further safeguarded with plastic bubble wrap on the top, sides, and bottom of the fruit. Further, they

state, “Packed product and packaging is to be protected from pestPest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 93 contamination during and after packing, after treatment, during storage and during movement b etween locations (e.g., packing house to cool storage/depot, to inspection point, to export point).” In the packinghouse, fruit should undergo mechanical brushing or other treatments to remove external pests. Immersion of the fruit in a water bath contain ing surfactant and, perhaps, a surface sterilant, such as chlorine bleach, could remove external pests . The liquid in the bath should be able to penetrate the residual floral material at the stem end, and contact and kill any arthropods that may

be conceal ed around the stem. Detergent - water solutions of about 1 ml/Lare effective in killing meal ybugs and other Coccoidea on plants ( Townsend, 1993 ) . Surfactants, such as common dishwashing detergent, may show a high degree of insecticidal activity with minimal likelihood of phytotoxicity . Additionally , leaves and stems should be removed from the fruit to eliminate pests associated with these plant parts, and to be consistent with U . S . regulations that imported fruit must be free of leaves and soil [ 7 CFR § 319. 56 , 2003] . All fruit should be inspected prior to packing . A random sample of fruit per lot should be inspected for external pests and cut to reveal internal pests; each sample is to be of a sufficient size in order to detect pest infestations . Because the deep cal yc es on pomegranates provide excellent shelter and hiding places for external pests, particular attention must be paid to them. Additionally , some fruit should be cut apart in order to detect internal infestations of fruit fly larvae and other internal feeders (CABI, 2006). During the grading process, any damaged, diseased, or infested fruit needs to be removed and separated from the commodity destined for export. It should be noted that visual inspection and culling are not considered to be appropriate risk management options by

themselves and must be used in combination with other measures. **2.2. 2**  **. Quarantine treatments**  There are currently

no specific quarantine treatments available for any of the above pests on pomegranate ( PPQ, 2008 ) . Approved pomegranate quarantine treatments (specificall y, cold treatment) exist only

for Ceratitis capitata, C. rosa, and Anastrepha spp., although treatments do exists for Bactroc era spp., and Conogethes punctiferalis, as well as the external pests (the soft scales, meal ybugs, thrips, and mites) on other commodities. There are currently no treatments available for the following pests: Cryptoblabes gnidiella, Deudorix spp., Dichocro cis megillalis, or the pathogens. An approved treatment to c ontrol hitchhiker surface pests (including Ceroplastes rubens , Drosicha dalbergiae, Icerya spp. , Rhipiphorothrips cruentatus, Scirtothrips spp. , and Tenuipalpus spp.) on various commodities (but not currentl y including pomegranate), is T104 - a 1 **fumigation** with Methyl
- Bromide at NAP

—tarpaulin or chamber. An approved treatment to control meal ybugs (Pseudococcidae) on various commodities (but not currently including pomegranate ) is T104 - a - 2 **fumig ation**  with Methyl
- Bromide at NAP

—tarpaulin or chamber. This treatment would supersede T104 - a - 1 because higher temperatures must be used during the same duration of the treatment. Recently , APHI S published a final rule establishing “a minimum generic dose of 150 Gy for allPest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 94 fruit flies of the family

Tephritidae,” and “a minimum generic dose of 400 Gy for all plant pests of the class Insecta other than pupae and adults of the order Lepidoptera” for “all regulated articles (i.e., fruits, vegetabl es, cut flowers, and foliage)” (Shea, 2006) . There are two exceptions to these generic doses: 1) “lower minimum doses for certain fruit flies,” and 2) “new approved minimum doses for 10 p lant pe sts” (Shea, 2006) . If irradiation is used, measures are to be implemented to ensure the phy tosanitary security of the consignments after treatment to prevent re - infestation. Irradiation may not kill all internal insects; the endpoint of treatment may be in activation, sterilization , or preventing maturation of larvae. Therefore , the presence of live larvae in a treated consignment would not necessarily result in its rejection, provided appropriate safeguarding measures had been used (IPPC, 200 7: I SPM #18 ) . The probit
- 9 - level security afforded by

a quarantine treatment may be overwhelmed by

a large volume of infested fruit (Powell, 2003). For this reason, adoption of a particular quarantine treatment is to be in conjunction with efforts to maintain pest populations in production zones below specified densities, such as those that would satisfy the requirements for the establishment of areas of low pest prevalence (IPPC, 200 7: I SPM #22 ). Lastly , fruit may be damaged or quality of the fruit reduced as a result of some of these treatments (e.g. irradiation may damage some fruits). **2.2. 3**  **. Limits on distribution and transit within the United States**  In some instances, the importation of commodities that might be harboring exotic pests may be authorized for shipment to specific locations ( e.g ., Alaska or North Atlantic ports) or during a specific season (usuall y the one with the coldest temperatures). These additional measures limit the likelihood of establishment for many exotic pests. In India, pomegranates are harvested from December through March and July through September. The majority of the quarantine pests are limited to tropical or subtropical distributions and/or have host ranges restricted to tropical/subtropical crops; however , the pests Conogethes punctiferalis, Cryptoblabes gnidiella, Deudorix epijarbas are capable of establishing in at least Plant Hardiness Zones 6
- 10, which encompass the majority of the United States. Limiting the distribution of pomegranates shipped July through September would not likely increase the level of protection to U.S. Agriculture, since these months are warm throughout the United States. However, it is possible that limiting the distribution of pomegranates shipped December through March to the northern states could p rovide some measure of protection and might be worth exploring. **2.3 .**  **Oversight 2.3.**  **1 .**  **Pre -**  **shipment programs** Inspection, treatment, or other risk mitigation measures performed in the grove and packinghouse are to be under the direct supervision of qualified APHIS and In dian Ministry of Agriculture personnel, and in accordance with specified phy tosanitary procedures. Such programs require monitoring all aspects of the application of any required phy

tosanitary measures and also aim to identify shortcomings or opportunities for program modifications (IPPC, 200 7: I SPM #14 ) . Provision needs to be made for the formal recognition of approved areas, sites, or producers, as well as the specification of conditions for revoking approvals or refusing certification for export to the United States.Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 95 **2.3. 2**  **. Point** **- of** 

**- entry sampling and inspection**  : If a preclearance program is not implemented, then inspection at port of entry is necessary . Upon arrival in the United States, consignm ents should be inspected, with particular attention given to paperwork and seals on vehicles, to ascertain that the chain of custody has remained intact. Each consignment should be inspected to detect a pest infestation/infection rate of 10 percent or greater. In the case of internal feeding insects such as fruit flies inspection should also include fruit cutting ( PPQ, 2008 ) . **2.3. 3**  **. Field Survey and Trapping**  : Survey procedures include visual inspection, fruit cutting, and trapping within and outside areas of production. Survey s are to be conducted at regular intervals during the growing season to determine the presence or absence of pests. Growers need to receive or be denied certification for export on the basis of survey or trapping results. **2.3. 4**  **. Ship**  **ments traceable to place of origin in India** : A requirement that pomegranate be packed in containers with identification labels indicating the specific place of origin may be necessary to ensure traceability

to each production site. **3 . Conclusions**  The number and diversity of pests potentially

infesting pomegranate imports make it unlikely that a single risk mitigation measure will be suff icient to adequatel y reduce likelihood of their introduction into the United States. For this reason, a combination of measures may be feasible including: grove monitoring ; management programs to achieve and maintain area pest freedom or low pest prevalence ; packinghouse inspection and treatments ; quarantine treatments ; and maintenance of consignment security and traceabil ity

in transit. These o ptions for risk mitigation are summarized below (Table A1) . The specific implementation of measures, such as those that would be included in an operational workplan, is beyond the scope of this document. This document does not purport to establish specific work plans or to evaluate the quality of a specific program or s ys tems approach. It identifies risks and provides information regarding known mitigative measures. The specification and implementation of measures, as would be described in an operational work plan, is beyond the scope of this document. **Table A1.** Summary

of risk mitigation options for pomegranates from India into the continental United States , including Alaska **Measures Pests**  **Efficacy** Pest - free areas or areas of low pest prevalence All (including pathogen) When conducted in compliance with international standards (e.g., IPPC, 2007: I SPM #s 4 and 10 ; NAPPO, 2004) this method satisfies requirements for appropriate level of protection Suppression /Control program Most of the pests of concern could be at least partly managed, with a control program, whether Research required on effectiveness of methods proposed.Pest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 96 **Measures Pests**  **Efficacy** cultural, chemical, or mechanical means, but monitoring and inspection are needed to verify program effectiveness. Control must be u sed in combination with other measures. Phy tosanitary Certification Inspection and Monitoring All (including pathogen), but must be used in combination with other measures. A phy tosanitary certifi cate guides inspections in India and alerts U.S. inspectors to pests of concern with this commodity . Packinghouse procedures, including visual culling External pests and pathogens Research required on efficacy . Point
- of
- entry sampling and inspection inc luding fruit cuttings Most external pests; most internal pests when fruit is cut For cut fruit, sample size needs to be adequate to detect infestation Irradiation combined T105 - b with low pest prevalence Bactrocera spp. Approved b y APHIS to treat fruit flies in apricot, peach, nectarine, and plum fruit. Irradiation, generic dose 400 Gy , combined with low pest prevalence All arthropods pests (with the exception of Lepidopteran pupae and adults) Approved b y APHIS Irradi ation 150 Gy

for fruit flies combined with low prevalence of other pests plus inspection All arthropod pests (fruit flies with irradiation, and other arthropods through areas of low pest prevalence) Research required to demonstrate efficacy Methyl bromid e fumigation T104 - a - 1 combined with low pest prevalence Ceroplastes rubens, Drosicha dalbergiae, Icerya spp ., Rhipiphorothrips cruentatus, Scirtothrips spp ., and Tenuipalpus spp Approved b y APHIS on various commodities for hitchhiker surface pests, such as thrips, scale insects, and spider mites. Research required to demonstrate efficacy for internal feeders (e.g., fruit fly larvae ) . Methyl bromide fumigation T104 - a - 2 combined with low pest prevalence Pseudococcidae Approved b y APHIS to treat mealy bugs on various commodities. Research required to demonstrat e efficacy

for external feeders. Port - of
- entry sampling and inspection All (including pathogen), but must be used in combination with other measuresPest Risk Assessment for Pomegranate from India

Rev. 04 August 17, 2010 97 **4 . Litera**  **ture Cited** 7 CFR § 319.28. U.S. Code of Federal Regulations, Title 7, Part 319 (7 CFR § 319.28

– Unshu oranges from Japan). 7 CFR § 319.56 . U.S. Code of Federal Regulations, Title 7, Part 319 (7 CFR § 319.56

– Notice of Quarantine). 7 CFR 319.56 - 24 . U.S. Code of Federal Regulations, Title 7, Part 319 (7 CFR § 319.56
- 2u

– Conditions governing the entry of lettuce and peppers from Israel). 7 CFR 319.56 - 28 . U.S. Code of Federal Regulations, Title 7, Part 319 (7 CFR § 319.56
- 2dd

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