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                            Funding & Awards
 Detection and remediation of PCR inhibition using real time PCR melt curves as a diagnostic tool.
Award Information
Awardee
              Florida International University
Award #
              2010-DN-BX-K204
Funding Category
              Competitive
Location
                    Miami, FL
Awardee County
              Dade
Congressional District
              26
Status
              Closed
Funding First Awarded
              2010
Total funding (to date)
              $294,800
Original Solicitation
              NIJ FY 10 Forensic DNA Research and Development
Description of original award (Fiscal Year 2010, $294,800)
This project builds on previously NIJ funded work to examine and characterize mechanisms for degradation and inhibition of DNA samples. The goal of this proposal to examine the capability of a commercially available real-time PCR system (Plexor HY) to assess and identify classes of inhibitors a priori through the use of melt curve effects. Researches propose to look at ways to enhance the capability of Plexor to detect inhibition by modifying the chemistry of the reaction mixture. Based on data generated from previous work, researchers also propose to develop better internal positive control sequence (IPC) probes that are more efficient at detecting inhibitors that bind DNA. Using this data researchers will categorize various inhibitors based on their effect on amplification of Identifiler and Powerplex 16, and ultimate plan to assess various methods to relieve inhibition including sample dilution, spin filtration, and magnetic bead based extraction.
ca/ncf



  
Grant-Funded Publications
        
                      Understanding Metal Inhibition: The Effect of Copper (Cu2+) on DNA Containing Samples
                  Use of Direct Analysis in Real Time (DART) To Assess the Levels of Inhibitors Co-extracted With DNA and the Associated Impact in Quantification and Amplification
                  The Use of Direct Analysis in Real Time (DART) To Assess the Levels of Inhibitors Co-extracted With DNA and the Associated Impact in Quantification and Amplification
                  Investigation of PCR Inhibition Using Plexor (R)-Based Quantitative PCR and Short Tandem Repeat Amplification
                  Effect of Internal Control Sequence and Length on the Response to PCR Inhibition in Real-Time PCR Quantitation
                  The Application of Magnetic Bead Hybridization for the Recovery and STR Amplification of Degraded and Inhibited Forensic DNA

  
Date Created: September 16, 2010
    
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