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                            Funding & Awards
 DNA Forensics Using Single Molecule Technology:  From DNA Recovery and Extraction to Genotyping Degraded and Trace Evidence without PCR
Award Information
Awardee
              The University of Kansas Center for Research, Inc.
Award #
              2011-DN-BX-K542
Funding Category
              Competitive
Location
                    Lawrence, KS
Awardee County
              Douglas
Congressional District
              2
Status
              Closed
Funding First Awarded
              2011
Total funding (to date)
              $587,597
Original Solicitation
              NIJ FY 11 Basic Scientific Research to Support Forensic Science for Criminal Justice Purposes
Description of original award (Fiscal Year 2011, $587,597)
DNA samples containing only trace amounts of DNA or DNA degraded by age, radiation, or chemical erosion will be studied.  By adapting the sensitivity and specificity of single molecule techniques to forensic analysis, low copy number and degraded samples can be imaged with a minimum of manipulation and little or no amplification. Such technology will make a large impact by providing means of interrogating samples which are currently beyond the reach of existing technology. To apply technologies originally developed for relatively pristine laboratory samples to forensics, specialized protocols will be developed. Issues of sample purity will be addressed by characterizing the effectiveness of current purification protocols and developing specialized protocols for the additional purification that may be necessary for single molecule investigations. Suitable methods of quantifying DNA markers will be determined. Possibilities include using florescence correlation spectroscopy, step-wise photodestruction of fluorophores, Atomic Force Microscopy imaging of lengths, and fluorescence localization experiments. In addition, single molecule techniques may be used to complement existing protocols. The ability to detect and measure small quantities can provide specific information about sample composition that investigators need to properly interpret the data. Finally, the basic science associated with this project can clarify how different contaminants inhibit PCR and will therefore suggest new protocols for sample treatment to make current molecular biology approaches more effective.

ca/ncf



  
Grant-Funded Publications
        
                      DNA Forensics Using Single Molecule Technology: From DNA Recovery and Extraction to Genotyping Degraded and Trace Evidence Without PCR

  
Date Created: September 11, 2011
    
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