Interlaboratory Analytical 

Comparison Study of Total Fatty Acid 

Concentrations in Human Serum:

Results for Exercise 01: 

QA12FASER01

Michele M. Schantz

Carissa D. Powers

Rosemary L. Schleicher

 NISTIR 7953
NISTIR 7953

Interlaboratory Analytical Comparison Study of Total 

Fatty Acid Concentrations in Human Serum:

Results for Exercise 01: QA12FASER01

Michele M. Schantz 

Chemical Sciences Division

Material Measurement Laboratory

Carissa D. Powers and Rosemary L. Schleicher

Centers for Disease Control and Prevention (CDC)

Atlanta, GA

September 2013

U.S. Department of Commerce

Penny Pritzker, Secretary

National Institute of Standards and Technology 

Patrick D. Gallagher, Deputy Secretary of Commerce and Director
ii

ABSTRACT

The National Institute of Standards and Technology (NIST), in conjunction with the Centers for Disease 

Control and Prevention (CDC) and NIH’s Office of Dietary Supplements (ODS), initiated an 

interlaboratory analytical comparison study of total fatty acid concentrations in human serum in May 

2012. The first sample was candidate Standard Reference Material (SRM) 2378. SRM 2378 consists of 

three levels of fatty acids in serum collected from: (1) donors who have not taken fish or flaxseed oil 

supplements for one month prior to collection; (2) donors who have taken flaxseed oil supplements for 

a minimum of one month prior to collection; and (3) donors who have taken fish oil supplements for a 

minimum of one month prior to collection. In addition, participants were requested to run SRM 1950 

Metabolites in Human Plasma as a control sample. This is a performance-based program so 

participating laboratories were requested to use the analytical procedures that they typically use in their 

laboratories for these analyses and report data for those fatty acids that they typically quantify. The 

results from the first exercise are reported along with a summary of the analytical methods used.
1

Overview of Exercise 01: QA12FASER01

For the first exercise of Interlaboratory Analytical Comparison Study of Total Fatty Acid Concentrations 

in Human Serum, candidate Standard Reference Material (SRM) 2378 was distributed as the study 

sample along with SRM 1950 Metabolites in Human Plasma which was distributed as the control sample. 

Candidate SRM 2378 consists of three serum materials collected from: donors who have not taken fish 

or flaxseed oil supplements for one month prior to collection; donors who have taken flaxseed oil 

supplements for a minimum of one month prior to collection; and donors who have taken fish oil 

supplements for a minimum of one month prior to collection. SRM 1950 is designed to represent 

“normal” human plasma. Plasma was obtained from 100 individuals (equal number of men and women 

in a narrow age range (40 to 50 years) who had undergone an overnight fast prior to blood draw. 

Certified and reference values are provided for selected fatty acids on the Certificate of Analysis for SRM 

1950(https://www-s.nist.gov/srmors/view_detail.cfm?srm=1950).

The laboratories were provided with four vials of each material and were requested to do triplicate 

measurements for each material (SRM 1950 and three levels of SRM 2378) using their laboratory's 

and/or program's analytical protocols, for the concentrations of the fatty acids currently being 

determined in their laboratory. A target list of compounds is presented in Table 1; however, 

participants did not need to quantify all of these compounds and could add additional compounds when 

reporting data. They were requested to report results, using three significant figures, in triplicate per 

analyte for each level of candidate SRM 2378 and for SRM 1950 in units of either μg/g or μmol/L and to 

provide brief descriptions of their cleanup and analytical procedures. 

REPORTED RESULTS

Laboratories were assigned numerical identification codes in order of receipt of data with the exception 

of the NIST laboratory which is Lab 1 in this exercise. The laboratory mean replicate data are shown in 

Tables 2 to 5 for SRM 2378-1, SRM 2378-2, SRM 2378-3, and SRM 1950, respectively. Included in these 

tables are the laboratory mean values, standard deviations, and relative standard deviations both in the 

units that were reported and in a common unit, μmol/L. Summaries of the methods used by each 

laboratory are in Appendix A as well as charts of the mean numerical results reported by each laboratory 

for each analyte in the exercise materials.

DISCUSSION

NIST, CDC, and NIH’s ODS contacted over 30 laboratories in early 2012 regarding participation in an 

interlaboratory analytical comparison study of total fatty acid concentrations in human serum. Of the 

laboratories contacted, 13 replied with interest in participating in this study and received samples in 

May 2012. The deadline for receipt of data was July 31, 2102. At that point, however, only seven 

laboratories had returned data so the deadline was extended with four additional laboratories 

submitting data. An alphabetical list of laboratories who returned data is given in Appendix B. Please 

note that the numerical codes in the following tables and figures are based on the receipt of the data 

and therefore do not follow the order in Appendix B.
2

Laboratories were requested to submit data for the fatty acids that they typically monitor in similar 

samples (suggested list in Table 1) for three subsamples of each sample received (SRM 1950 and three 

levels of candidate SRM 2378) in units of either μg/g or μmol/L along with the density of each sample. 

Summaries of the results submitted by each laboratory are presented in Tables 2 through 5 for SRM 

2378-1, SRM 2378-2, SRM 2378-3, and SRM 1950, respectively. In Table 5, the certified and reference 

values for SRM 1950 are also summarized for comparison. The data from each laboratory are presented

in the tables as the means, standard deviations, and relative standard deviations for the three values 

submitted. Four laboratories reported the data in μg/g (shown in red in Tables 2 through 5). These data 

were converted to μmol/L using the densities provided by each laboratory except for lab 9. Lab 9 did 

not report a density so the density was assumed to be 1 g/mL for the conversion to μmol/L. 

The relative standard deviations (RSDs) for the laboratory data are generally < 20 %. Lab 3 had a few 

analytes with higher RSDs (up to 41 %) as did lab 4 (up to 36 %), lab 7 (up to 60 %), lab 8 (up to 31 %), 

and lab 9 (up to 27 %), but these were generally based on a limited number of analytes in each sample. 

Labs 3 and 4 reported data for three fatty acids not included in Table 1, Lab 7 reported data for nine 

additional fatty acids, and Lab 11 reported data for 10 additional fatty acids. The additional analytes and 

data are summarized in Table 6.

The data are plotted by analyte in material in the chart section of Appendix A. Each data point is the 

mean and associated standard deviation of the data reported by the laboratory of the indicated analyte

in the indicated sample. The medians calculated from the data received for the fatty acid in each 

sample are summarized in Table 7 and included for each chart in a text box. Note that after an initial 

look at the data, Lab 8’s data were not included in the median calculations since their data were 

substantially lower (in many cases an order of magnitude lower) than the consensus data from the other 

laboratories. The charts for SRM 1950 also include the reference or certified values in the chart title for 

comparison purposes. 

In addition to nomenclature, Table 1 summarizes the number of laboratories reporting data for each 

fatty acid in each material, the range of mean values reported, and the lab number reporting the highest 

and lowest values for each fatty acid in each material. Note that lab 8 was included in the summation of 

the number of laboratories reporting data (including LOD and LOQ) but not in the range of values or 

identification of lab number reporting the lowest value. Excluding lab 8’s data, there are no trends in 

the data across all fatty acids. There are a few trends with individual fatty acid as can be noted in Table 

1. For example, lab 10 reported the lowest mean value for myristic acid and myristoleic acid, and lab 2 

reported the highest mean value for cis-vaccenic acid and arachidic acid. 

This information is also presented in Table 8 as the percent difference for each laboratory mean (n=3) 

from the median data for the particular analyte in the indicated sample. There do appear to be certain

trends for individual fatty acids as noted above with data from one or more laboratories being high or 

low for a particular fatty acid in all of the materials studied. These results could be obtained by 

incomplete extraction (low), coelution with other fatty acids or matrix components on the analytical 

column used (high), or from some other method variations such as inaccurate calibration. The methods 

used by each laboratory are summarized in Appendix A with the procedures in Table A-1 and the 
3

calibration information in Table A-2. The hydrolysis and extraction methods were generally different 

among the laboratories with the exception being labs 3 and 4 reporting the use of very similar 

procedures. All laboratories used gas chromatography (GC) with either flame ionization detection (FID) 

or mass spectrometry (MS) following a derivatization. For the calibration information (Table A-2), most 

laboratories used a linear regression with the exception of lab 6 which used a quadratic regression, lab 9 

which reported only a weighting factor, lab 10 which did a one point calibration, and lab 11 used a mix 

of linear and quadratic regression tailored by analyte. Labs 5 and 7 did not report calibration 

information. 

Intercomparison exercises provide an important mechanism for assessing the comparability, 

repeatability, and trueness of data being produced by the participating laboratories. Exercise materials 

similar in matrix, form, and analyte concentration to typical samples routinely analyzed by the 

laboratories are most useful for demonstrating the level of comparability and for revealing potential 

measurement and method problems. The data from this exercise can be used to assess the 

comparability across a limited number of international laboratories (see Appendix B). The data do not 

indicate specific method biases but do indicate the need for such intercomparisons and for the use of 

control materials to increase the comparability of data across laboratories. 

Acknowledgments

The time and effort of the analysts and management of the participating laboratories are gratefully 

acknowledged. 

Disclaimer

Certain commercial equipment, instruments, or materials are identified in this report to specify 

adequately the experimental procedure. Such identification does not imply recommendation or 

endorsement by the National Institute of Standards and Technology, nor does it imply that the materials 

or equipment identified are the best available for the purpose.
4

Table 1. Nomenclature used in this study and summary of values reported

mol wt # Labs range reported lab reporting lab reporting

g/mol material reporting (μmol/L) highest valuea lowest valuea

C14:0 Myristic acid 228.38 SRM 2378-1 11 128-286 7 10

SRM 2378-2 11 86.4-175 5 10

SRM 2378-3 11 88.2-224 7 10

SRM 1950 11 61.4-162 7 10

C14:1n5 Myristoleic acid 226.38 SRM 2378-1 8 7.31-28.5 7 10

SRM 2378-2 8 5.49-19.3 2 10

SRM 2378-3 8 5.69-24.5 7 10

SRM 1950 8 4.31-16.5 7 10

C16:0 Palmitic acid 256.43 SRM 2378-1 11 1473-3884 7 2

SRM 2378-2 11 1523-3680 5 2

SRM 2378-3 11 1307-3186 7 2

SRM 1950 11 1273-3058 7 2

C16:1n7 Palmitoleic acid 254.43 SRM 2378-1 11 55.1-252 5 7

SRM 2378-2 11 63.9-333 5 7

SRM 2378-3 11 58.6-228 5 7

SRM 1950 11 61.6-338 5 7

C18:0 Stearic acid 284.48 SRM 2378-1 11 694-1154 7 2

SRM 2378-2 11 696-1122 5 10

SRM 2378-3 11 587-1069 7 10

SRM 1950 11 526-932 7 10

C18:1n7 cis- Vaccenic acid 282.48 SRM 2378-1 9 118-234 2 3

SRM 2378-2 9 109-264 2 3

SRM 2378-3 9 92.5-212 2 3

SRM 1950 9 129-295 2 1

C18:1n9 Oleic acid 282.48 SRM 2378-1 11 1113-2788 7 2

SRM 2378-2 11 1303-3285 5 2

SRM 2378-3 11 1077-2603 7 2

SRM 1950 11 1113-2513 7 2

C18:2n6 Linoleic acid 280.48 SRM 2378-1 11 2631-4228 7 10

SRM 2378-2 11 3180-5328 5 7

SRM 2378-3 11 2331-4024 5 10

SRM 1950 11 2081-3776 7 10

C18:3n3 alpha- Linolenic acid 278.48 SRM 2378-1 11 79.2-147 7 4

SRM 2378-2 11 78.7-130 5 10

SRM 2378-3 11 40.8-84.6 7 4

SRM 1950 11 36.6-76.2 7 10

C18:3n6 gamma- Linolenic acid 278.48 SRM 2378-1 11 30.8-57.5 7 10

SRM 2378-2 11 54.4-93.9 5 10

SRM 2378-3 11 34.0-66.2 7 10

SRM 1950 11 36.6-72.9 7 10

C20:0 Arachidic acid 312.54 SRM 2378-1 10 15.8-68.0 2 4

SRM 2378-2 10 15.2-78.3 2 4

SRM 2378-3 10 12.5-59.0 2 4

SRM 1950 10 11.5-51.7 2 4

C20:1n9 11-Eicosenoic acid 310.54 SRM 2378-1 9 11.8-48.1 7 4

SRM 2378-2 9 12.2-37.3 7 10

SRM 2378-3 9 13.1-29.1 7 10

SRM 1950 9 10.3-27.4 7 4
5

Table 1 (cont). Nomenclature used in this study and summary of values reported

mol wt # Labs range reported reported reported

g/mol material reporting (μmol/L) highest valuea lowest valuea

C20:2n6 11,14-Eicosadienoic acid 308.53 SRM 2378-1 8 8.50-19.8 3 7

SRM 2378-2 8 5.98-25.8 5 7

SRM 2378-3 8 6.59-22.5 5 7

SRM 1950 8 7.78-22.4 5 7

C20:3n6 homo-gamma- Linolenic acid 306.53 SRM 2378-1 10 59.3-158 7 2

SRM 2378-2 10 89.3-231 5 2

SRM 2378-3 10 62.0-176 7 2

SRM 1950 10 68.0-204 7 2

C20:4n6 Arachidonic acid 304.52 SRM 2378-1 11 493-987 2 4

SRM 2378-2 11 646-1330 2 10

SRM 2378-3 11 569-1127 2 10

SRM 1950 11 540-1067 2 10

C20:5n3 Eicosapentaenoic acid (EPA) 302.52 SRM 2378-1 11 228-370 2 10

SRM 2378-2 11 62.6-115 2 10

SRM 2378-3 11 50.8-87.0 2 and 7 10

SRM 1950 11 35.8-61.7 7 10

C22:0 Docosanoic acid 340.59 SRM 2378-1 11 5.02-67.1 10 7

SRM 2378-2 11 2.37-92.7 11 7

SRM 2378-3 11 3.53-61.0 11 7

SRM 1950 11 2.95-52.1 11 7

C22:1n9 Docosenoic acid 338.59 SRM 2378-1 7 3.24-19 9 10

SRM 2378-2 7 2.20-16.3 9 10

SRM 2378-3 7 2.09-19.9 9 10

SRM 1950 7 2.12-20.7 9 10

C22:4n6 Docosatetraenoic acid 332.57 SRM 2378-1 10 9.00-29.9 9 2

SRM 2378-2 10 17.7-41.8 9 2

SRM 2378-3 10 12.3-32.6 9 2

SRM 1950 10 12.7-41.2 7 5

C22:5n3 Docosapentaenoic acid 330.57 SRM 2378-1 11 52.0-99.8 7 2 and 4

SRM 2378-2 11 39.0-55.9 5 4

SRM 2378-3 11 27.0-50.6 7 4

SRM 1950 11 32.7-59.5 7 2

C22:5n6 Docosapentaenoic acid 330.57 SRM 2378-1 9 6.00-12.8 7 2

SRM 2378-2 9 9.67-16.7 7 2

SRM 2378-3 9 8.67-21.1 7 2

SRM 1950 9 11.7-27.3 7 2

C22:6n3 Docosahexaenoic acid (DHA) 328.57 SRM 2378-1 11 241-450 7 4

SRM 2378-2 11 136-195 7 10

SRM 2378-3 11 124-251 7 4

SRM 1950 11 82.3-170 7 10

C24:0 Lignoceric acid 368.64 SRM 2378-1 11 3.77-70.0 10 7

SRM 2378-2 11 2.61-81.9 10 7

SRM 2378-3 11 4.10-56.5 10 7

SRM 1950 11 2.47-47.3 10 7

C24:1n9 Nervonic acid 366.63 SRM 2378-1 10 18.2-104 10 3

SRM 2378-2 10 20.4-86.4 11 4

SRM 2378-3 10 13.1-63.8 10 4

SRM 1950 10 16.0-72.1 10 3

a

 Excluding data submitted by Lab 8
6

Table 2. Interlaboratory data received for SRM 2378-1

units reported μg/g μmol/L μmol/L μmol/L μg/g

Lab 1 Lab 2 Lab 3 Lab 4 Lab 5

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

C14:0 47.2 1.7 3.68% 196 4 2.04% 188 5 2.81% 155 5 2.94% 50.5 0.8 1.49%

C14:1n5 4.12 0.20 4.77% 21.7 1.2 5.33% NA 3.22 0.09 2.80%

C16:0 918 26 2.83% 1473 15 1.04% 2650 62 2.35% 2822 59 2.10% 936 18 1.92%

C16:1n7 53.1 3.8 7.16% 207 12 5.59% 187 8 4.21% 196 6 3.26% 65.3 1.5 2.30%

C18:0 240 5 1.91% 694 14 2.05% 737 20 2.69% 761 18 2.39% 280 4.2 1.50%

C18:1n7 46.7 4.0 8.51% 234 12 5.00% 118 5 4.41% NA 57.9 1.0 1.73%

C18:1n9 550 14 2.59% 1113 25 2.26% 1776 34 1.90% 1830 67 3.66% 691 11 1.59%

C18:2n6 1099 6 0.51% 3403 68 2.00% 3092 55 1.79% 2738 163 5.95% 1150 20 1.74%

C18:3n3 35.1 3.1 8.93% 104 1 0.55% 89.0 2.7 3.04% 79.2 1.2 1.50% 34.5 0.7 1.88%

C18:3n6 13.3 0.7 5.12% 42.0 1.0 2.38% 38.7 1.5 3.91% 37.7 1.9 5.00% 14.7 0.3 1.97%

C20:0 6.65 0.34 5.07% 68.0 2.0 2.94% 27.2 1.2 4.29% 15.8 0.9 5.79% 6.56 0.15 2.29%

C20:1n9 5.48 0.35 6.43% NA 18.0 3.9 21.85% 11.8 0.7 5.57% 5.35 0.05 0.93%

C20:2n6 NA NA 19.8 0.9 4.55% 19.7 1.3 6.58% 6.19 0.28 4.52%

C20:3n6 NA 59.3 1.5 2.57% 101 5 5.36% 87.8 3.4 3.85% 37.7 0.3 0.66%

C20:4n6 209 8 3.98% 987 10 1.03% 528 15 2.91% 493 29 5.84% 223 2 0.94%

C20:5n3 93.8 1.7 1.84% 370 7 1.99% 244 11 4.57% 236 10 4.33% 94.3 0.7 0.70%

C22:0 14.9 0.1 0.91% 42.3 0.6 1.30% 16.0 1.0 6.52% 21.5 4.3 20.27% 14.6 1.0 7.12%

C22:1n9 2.51 0.24 9.66% 7.33 0.58 7.87% NA NA 2.52 0.49 19.44%

C22:4n6 NA 9.00 0.00 0.00% 11.5 1.1 9.56% 10.3 0.7 6.83% 5.53 0.70 12.66%

C22:5n3 20.4 0.8 3.90% 52.0 7.5 14.52% 65.0 4.2 6.44% 52.0 1.8 3.44% 24.4 0.5 2.05%

C22:5n6 NA 6.00 0.00 0.00% 7.32 2.54 34.66% 8.00 0.73 9.15% 3.10 0.08 2.58%

C22:6n3 108 3 2.66% 356 3 0.74% 287 7 2.40% 241 8 3.47% 111 2 1.35%

C24:0 14.3 0.7 5.16% 46.5 0.3 0.62% 12.4 2.3 18.36% 9.42 1.16 12.28% 14.3 0.9 6.29%

C24:1n9 21.3 1.2 5.45% 63.3 1.5 2.41% 18.2 2.9 15.90% 21.7 6.4 29.69% 21.0 1.6 7.62%

Table 2 (Continued) Interlaboratory data received for SRM 2378-1

units reported μmol/L μg/g μmol/L μg/g μmol/L μmol/L

Lab 6 Lab 7 Lab 8 Lab 9 Lab 10 Lab 11

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

C14:0 189 4 2.20% 65.6 2.4 3.67% 17.3 0.3 1.93% 44.8 0.493821 1.10% 128 3 2.72% 198 1 0.54%

C14:1n5 NA 6.48 0.66 10.14% <LOD 3.51 0.07 1.91% 7.31 0.66 8.98% 12.3 0.3 2.58%

C16:0 2977 74 2.48% 1000 13 1.30% 387 29 7.61% 774 7 0.84% 2290 37 1.62% 3113 20 0.64%

C16:1n7 210 4 1.98% 14.1 1.4 9.94% 6.04 0.93 15.39% 53.1 0.5 1.00% 150 3 2.26% 207 2 0.74%

C18:0 806 4 0.45% 330 8 2.30% 102 5 5.25% 240 2 0.71% 696 15 2.18% 714 9 1.26%

C18:1n7 143 3 1.85% 42.6 11.3 26.54% 43.3 7.4 17.12% ND 138 3 2.19% 136 3 1.97%

C18:1n9 2017 55 2.73% 791 25 3.12% 71.7 5.6 7.80% 636 5 0.71% 1602 23 1.42% 2076 19 0.92%

C18:2n6 3387 112 3.32% 1191 2 0.13% 130 41 31.08% 955 7 0.77% 2631 29 1.10% 3422 32 0.94%

C18:3n3 104 2 2.01% 41.1 0.5 1.14% <LOD 30.5 0.2 0.78% 80.6 0.4 0.51% 112 1 0.46%

C18:3n6 40.8 0.9 2.23% 16.1 0.8 4.94% 5.18 0.96 18.45% 12.1 0.0 0.11% 30.8 0.3 1.03% 40.3 0.2 0.48%

C20:0 NA 8.76 0.46 5.25% <LOD 8.59 0.66 7.64% 25.9 0.6 2.48% 24.9 0.5 1.89%

C20:1n9 NA 15.0 8.7 57.67% <LOD 4.96 0.27 5.36% 16.2 0.3 1.71% 16.3 0.2 1.21%

C20:2n6 NA 2.63 0.58 22.14% <LOD 6.04 0.25 4.19% 14.0 0.6 4.04% 17.4 0.4 2.41%

C20:3n6 104 2 1.46% 48.8 0.2 0.39% 6.53 0.96 14.68% 32.8 0.2 0.54% 94.8 2.5 2.61% 102 1 1.37%

C20:4n6 625 13 2.06% 277 3 0.99% 29.9 2.3 7.55% 194 1 0.41% 533 9 1.68% 634 3 0.53%

C20:5n3 257 5 2.06% 112 0 0.13% 9.83 1.09 11.13% 81.4 0.6 0.71% 228 2 0.96% 272 7 2.41%

C22:0 44.5 1.1 2.47% 1.72 0.35 20.38% <LOD 15.7 1.3 8.08% 67.1 0.8 1.24% 58.7 0.8 1.29%

C22:1n9 NA <LOD 6.42 0.71 10.99% 3.24 0.26 7.91% 4.21 0.62 14.70%

C22:4n6 12.4 0.2 1.40% 7.18 0.66 9.19% <LOD 9.93 0.91 9.19% 13.1 0.1 0.97% 12.4 0.11 0.85%

C22:5n3 66.2 1.4 2.12% 33.1 1.0 3.14% <LOQ 21.8 0.5 2.30% 65.7 2.3 3.50% 70.5 0.5 0.64%

C22:5n6 NA 4.26 0.23 5.40% <LOD 3.01 0.09 3.12% 8.00 0.32 4.00% 8.37 0.26 3.06%

C22:6n3 283 7 2.47% 149 2 1.31% 13.0 0.8 6.34% 98.2 2.5 2.56% 276 7 2.45% 320 6 1.96%

C24:0 42.2 0.5 1.09% 1.39 0.24 17.00% <LOD 15.7 1.5 9.78% 70.0 1.1 1.55% 56.2 0.4 0.39%

C24:1n9 62.5 2.7 4.28% <LOD 21.0 1.6 7.66% 104 1 1.43% 87.1 0.8 0.80%

NOTE for Lab 9:

C18:1n9 Oleic acid Total 18:1 determined & entered

C20:1n9 11-Eicosenoic acid Total 20:1 determined & entered

C22:1n9 Docosenoic acid Total 22:1 determined & entered
7

Table 2 (Continued) Interlaboratory data received for SRM 2378-1

Lab 1 Lab 2 Lab 3 Lab 4 Lab 5

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

density (g/mL) 1.02 0.00 0.01% not reported 0.989 0.030 3.06% not reported 0.983 0.035 3.56%

CONVERT ALL TO μmol/L

Lab 1 Lab 2 Lab 3 Lab 4 Lab 5

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

C14:0 211 8 3.68% 196 4 2.04% 188 5 2.81% 155 5 2.94% 217 3 1.49%

C14:1n5 18.6 0.9 4.77% 21.7 1.2 5.33% NA 14.0 0.4 2.80%

C16:0 3661 103 2.83% 1473 15 1.04% 2650 62 2.35% 2822 59 2.10% 3588 69 1.92%

C16:1n7 213 15 7.16% 207 12 5.59% 187 8 4.21% 196 6 3.26% 252 6 2.30%

C18:0 863 16 1.91% 694 14 2.05% 737 20 2.69% 761 18 2.39% 968 15 1.50%

C18:1n7 169 14 8.51% 234 12 5.00% 118 5 4.41% NA 201 3 1.73%

C18:1n9 1990 51 2.59% 1113 25 2.26% 1776 34 1.90% 1830 67 3.66% 2405 38 1.59%

C18:2n6 4007 20 0.51% 3403 68 2.00% 3092 55 1.79% 2738 163 5.95% 4030 70 1.74%

C18:3n3 129 12 8.93% 104 1 0.55% 89.0 2.7 3.04% 79.2 1.2 1.50% 122 2 1.88%

C18:3n6 48.9 2.5 5.12% 42.0 1.0 2.38% 38.7 1.5 3.91% 37.7 1.9 5.00% 51.9 1.0 1.97%

C20:0 21.7 1.1 5.07% 68.0 2.0 2.94% 27.2 1.2 4.29% 15.8 0.9 5.79% 20.6 0.5 2.29%

C20:1n9 18.1 1.2 6.43% NA 18.0 3.9 21.85% 11.8 0.7 5.57% 16.9 0.2 0.93%

C20:2n6 NA NA 19.8 0.9 4.55% 19.7 1.3 6.58% 19.7 0.9 4.52%

C20:3n6 NA 59.3 1.5 2.57% 101 5 5.36% 87.8 3.4 3.85% 121 1 0.66%

C20:4n6 703 28 3.98% 987 10 1.03% 528 15 2.91% 493 29 5.84% 720 7 0.94%

C20:5n3 317 6 1.84% 370 7 1.99% 244 11 4.57% 236 10 4.33% 306 2 0.70%

C22:0 44.8 0.4 0.91% 42.3 0.6 1.30% 16.0 1.0 6.52% 21.5 4.3 20.27% 42.1 3.0 7.12%

C22:1n9 7.57 0.73 9.66% 7.33 0.58 7.87% NA NA 7.32 1.42 19.44%

C22:4n6 NA 9.00 0.00 0.00% 11.5 1.1 9.56% 10.3 0.7 6.83% 16.3 2.1 12.66%

C22:5n3 63.2 2.5 3.90% 52.0 7.5 14.52% 65.0 4.2 6.44% 52.0 1.8 3.44% 72.6 1.5 2.05%

C22:5n6 NA 6.00 0.00 0.00% 7.32 2.54 34.66% 8.00 0.73 9.15% 9.22 0.24 2.58%

C22:6n3 337 9 2.66% 356 3 0.74% 287 7 2.40% 241 8 3.47% 332 4 1.35%

C24:0 39.6 2.0 5.16% 46.5 0.3 0.62% 12.4 2.3 18.36% 9.42 1.16 12.28% 38.1 2.4 6.29%

C24:1n9 59.3 3.2 5.45% 63.3 1.5 2.41% 18.2 2.9 15.90% 21.7 6.4 29.69% 56.3 4.3 7.62%

Table 2 (Continued) Interlaboratory data received for SRM 2378-1

Lab 6 Lab 7 Lab 8 Lab 9 Lab 10 Lab 11

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

density (g/mL) 1.04 0.00 0.41% 0.996 0.010 0.95% 1.00 0.00 0.01% not reported use 1 0.967 0.005 0.52%

CONVERT ALL TO μmol/L

Lab 6 Lab 7 Lab 8 Lab 9 Lab 10 Lab 11

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

C14:0 189 4 2.20% 286 11 3.67% 17.3 0.3 1.93% 196 2 1.10% 128 3 2.72% 198 1 0.54%

C14:1n5 NA 28.5 2.9 10.14% <LOD 15.5 0.3 1.91% 7.31 0.66 8.98% 12.3 0.3 2.58%

C16:0 2977 74 2.48% 3884 50 1.30% 387 29 7.61% 3019 25 0.84% 2290 37 1.62% 3113 20 0.64%

C16:1n7 210 4 1.98% 55.1 5.5 9.94% 6.04 0.93 15.39% 209 2 1.00% 150 3 2.26% 207 2 0.74%

C18:0 806 4 0.45% 1154 27 2.30% 102 5 5.25% 842 6 0.71% 696 15 2.18% 714 9 1.26%

C18:1n7 143 3 1.85% 150 40 26.54% 43.3 7.4 17.12% NA 138 3 2.19% 136 3 1.97%

C18:1n9 2017 55 2.73% 2788 87 3.12% 71.7 5.6 7.80% 2250 16 0.71% 1602 23 1.42% 2076 19 0.92%

C18:2n6 3387 112 3.32% 4228 6 0.13% 130 41 31.08% 3403 26 0.77% 2631 29 1.10% 3422 32 0.94%

C18:3n3 104 2 2.01% 147 2 1.14% <LOD 110 1 0.78% 80.6 0.4 0.51% 112 1 0.46%

C18:3n6 40.8 0.9 2.23% 57.5 2.8 4.94% 5.18 0.96 18.45% 43.5 0.0 0.11% 30.8 0.3 1.03% 40.3 0.2 0.48%

C20:0 NA 27.9 1.5 5.25% <LOD 27.5 2.1 7.64% 25.9 0.6 2.48% 24.9 0.5 1.89%

C20:1n9 NA 48.1 27.7 57.67% <LOD 16.0 0.9 5.36% 16.2 0.3 1.71% 16.3 0.2 1.21%

C20:2n6 NA 8.50 1.88 22.14% <LOD 19.6 0.8 4.19% 14.0 0.6 4.04% 17.4 0.4 2.41%

C20:3n6 104 2 1.46% 158 1 0.39% 6.53 0.96 14.68% 107 1 0.54% 94.8 2.5 2.61% 102 1 1.37%

C20:4n6 625 13 2.06% 908 9 0.99% 29.9 2.3 7.55% 637 3 0.41% 533 9 1.68% 634 3 0.53%

C20:5n3 257 5 2.06% 368 0 0.13% 9.83 1.09 11.13% 269 2 0.71% 228 2 0.96% 272 7 2.41%

C22:0 44.5 1.1 2.47% 5.02 1.02 20.38% <LOD 46.2 3.7 8.08% 67.1 0.8 1.24% 58.7 0.8 1.29%

C22:1n9 NA <LOD 19.0 2.1 10.99% 3.24 0.26 7.91% 4.21 0.62 14.70%

C22:4n6 12.4 0.2 1.40% 21.5 2.0 9.19% <LOD 29.9 2.7 9.19% 13.1 0.1 0.97% 12.4 0.11 0.85%

C22:5n3 66.2 1.4 2.12% 99.8 3.1 3.14% <LOQ 66.1 1.5 2.30% 65.7 2.3 3.50% 70.5 0.5 0.64%

C22:5n6 NA 12.8 0.7 5.40% <LOD 9.12 0.28 3.12% 8.00 0.32 4.00% 8.37 0.26 3.06%

C22:6n3 283 7 2.47% 450 6 1.31% 13.0 0.8 6.34% 299 8 2.56% 276 7 2.45% 320 6 1.96%

C24:0 42.2 0.5 1.09% 3.77 0.64 17.00% <LOD 42.5 4.2 9.78% 70.0 1.1 1.55% 56.2 0.4 0.39%

C24:1n9 62.5 2.7 4.28% <LOD 57.3 4.4 7.66% 104 1 1.43% 87.1 0.8 0.80%

NOTE for Lab 9:

C18:1n9 Oleic acid Total 18:1 determined & entered

C20:1n9 11-Eicosenoic acid Total 20:1 determined & entered

C22:1n9 Docosenoic acid Total 22:1 determined & entered
8

Table 3. Interlaboratory data received for SRM 2378-2

units reported μg/g μmol/L μmol/L μmol/L μg/g

Lab 1 Lab 2 Lab 3 Lab 4 Lab 5

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

C14:0 35.4 2.4 6.82% 158 1 0.45% 153 8 5.41% 126 8 6.13% 39.6 2.0 5.16%

C14:1n5 3.38 0.28 8.18% 19.3 0.6 2.99% NA 2.55 0.12 4.71%

C16:0 719 22 3.13% 1523 32 2.11% 2811 122 4.35% 2762 142 5.15% 935 26 2.78%

C16:1n7 71.5 1.9 2.72% 264 13 4.74% 262 16 5.99% 242 11 4.57% 84.0 2.1 2.46%

C18:0 246 7 2.88% 785 22 2.80% 864 29 3.41% 848 55 6.49% 316 10 3.01%

C18:1n7 31.5 1.4 4.48% 264 4 1.33% 109 7 6.46% NA 62.0 0.8 1.32%

C18:1n9 707 10 1.36% 1303 64 4.93% 2611 152 5.81% 2461 214 8.68% 919 13 1.41%

C18:2n6 1194 51 4.24% 4067 64 1.58% 4378 255 5.83% 3615 415 11.48% 1480 20 1.35%

C18:3n3 31.0 1.2 3.77% 116 5 3.88% 96.6 6.8 7.04% 86.6 9.5 10.99% 36.0 0.5 1.25%

C18:3n6 20.4 1.0 4.82% 82.0 4.6 5.59% 71.3 7.7 10.81% 65.0 5.6 8.58% 25.9 0.3 1.24%

C20:0 7.05 0.37 5.20% 78.3 2.3 2.95% 28.7 3.8 13.15% 15.2 3.5 23.05% 7.39 0.66 8.93%

C20:1n9 4.92 0.23 4.63% NA 20.7 2.4 11.59% 12.3 1.2 9.45% 5.24 0.20 3.72%

C20:2n6 NA NA 24.5 1.8 7.40% 17.4 0.8 4.82% 7.90 0.15 1.90%

C20:3n6 NA 89.3 1.2 1.29% 200 11 5.42% 167 14 8.36% 70.1 0.6 0.84%

C20:4n6 225 8 3.61% 1330 56 4.19% 746 36 4.88% 668 72 10.74% 293 3 0.86%

C20:5n3 21.9 0.6 2.67% 115 3 2.79% 64.4 9.1 14.10% 76.2 6.3 8.28% 24.7 0.2 0.81%

C22:0 21.1 1.3 5.99% 59.2 0.3 0.54% 27.8 6.2 22.35% 33.9 5.2 15.32% 21.2 2.0 9.42%

C22:1n9 2.50 0.06 2.54% 7.00 0.00 0.00% NA NA 3.15 0.12 3.81%

C22:4n6 NA 17.7 0.6 3.27% 21.0 3.2 15.04% 17.8 2.3 12.94% 7.66 1.03 13.45%

C22:5n3 17.8 0.2 0.87% 45.3 4.0 8.91% 49.3 3.8 7.81% 39.0 1.7 4.30% 18.3 0.5 2.73%

C22:5n6 NA 9.67 1.53 15.80% 12.0 1.5 12.18% 13.4 0.7 4.84% 5.13 0.20 3.90%

C22:6n3 58.0 3.0 5.19% 184 13 7.11% 163 10 6.28% 145 2 1.43% 59.3 0.4 0.61%

C24:0 16.1 1.9 11.57% 60.2 1.5 2.45% 18.4 7.2 39.06% 21.2 0.9 4.42% 17.1 2.2 13.01%

C24:1n9 17.6 1.0 5.83% 67.3 2.1 3.09% 21.9 9.0 41.35% 20.4 6.0 29.67% 18.8 1.9 9.88%

Table 3 (continued) Interlaboratory data received for SRM 2378-2

units reported μmol/L μg/g μmol/L μg/g μmol/L μmol/L

Lab 6 Lab 7 Lab 8 Lab 9 Lab 10 Lab 11

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

C14:0 142 3 2.11% 35.0 3.4 9.60% 19.9 1.0 4.80% 34.3 1.1 3.20% 86.4 0.8 0.88% 152 1 0.63%

C14:1n5 NA 3.42 0.31 8.95% <LOD 2.75 0.19 6.86% 5.49 0.62 11.35% 9.24 0.16 1.68%

C16:0 2963 87 2.95% 717 85 11.81% 299 16 5.24% 768 31 4.07% 2057 15 0.72% 3151 20 0.62%

C16:1n7 266 7 2.56% 16.3 1.7 10.47% 8.08 0.81 10.07% 65.7 2.8 4.20% 182 3 1.80% 264 4 1.48%

C18:0 905 12 1.33% 285 27 9.47% 102 10 9.86% 264 12 4.69% 696 4 0.64% 808 3 0.38%

C18:1n7 146 0 0.00% 33.5 3.1 9.13% 36.2 2.4 6.55% ND 130 8 6.06% 140 1 0.90%

C18:1n9 2680 85 3.19% 702 87 12.45% 127 7 5.79% 827 29 3.51% 1984 23 1.17% 2764 23 0.84%

C18:2n6 4353 172 3.96% 896 100 11.12% 100 6 6.44% 1221 41 3.38% 3200 40 1.24% 4479 37 0.81%

C18:3n3 108 2 1.85% 29.9 4.7 15.80% <LOD 31.2 1.7 5.36% 78.7 0.7 0.85% 118 1 1.25%

C18:3n6 74.3 1.2 1.62% 15.3 2.3 14.92% 5.47 0.29 5.26% 21.2 0.7 3.54% 54.4 1.1 2.07% 76.3 0.7 0.87%

C20:0 NA 7.84 1.12 14.25% <LOD 10.6 1.6 15.29% 26.1 0.4 1.64% 29.4 0.3 0.84%

C20:1n9 NA 11.6 3.82 32.85% <LOD 4.46 0.53 11.95% 12.2 0.4 3.13% 16.0 0.3 1.96%

C20:2n6 NA 1.86 0.51 27.55% <LOD 7.66 0.45 5.92% 19.4 0.5 2.82% 23.1 0.2 0.87%

C20:3n6 189 3 1.52% 64.5 7.1 11.06% 7.94 0.50 6.32% 58.1 3.3 5.62% 156 2 1.25% 196 7 3.35%

C20:4n6 823 17 2.07% 239 26 10.89% 33.9 1.8 5.20% 247 15 6.03% 646 9 1.42% 856 8 0.94%

C20:5n3 68.5 1.1 1.55% 20.7 1.9 8.95% <LOD 20.4 1.5 7.59% 62.6 0.2 0.30% 71.9 0.5 0.73%

C22:0 69.7 1.9 2.66% 0.810 0.072 8.89% <LOD 22.7 3.8 16.55% 88.6 1.6 1.85% 92.7 1.2 1.30%

C22:1n9 NA <LOD 5.52 1.11 20.05% 2.20 0.31 14.02% 4.46 0.32 7.19%

C22:4n6 21.3 0.8 3.79% 9.14 0.85 9.24% <LOD 13.9 0.7 5.23% 20.2 0.5 2.55% 23.0 1.3 5.81%

C22:5n3 48.3 1.1 2.28% 17.8 2.7 15.16% <LOD 15.3 0.9 6.03% 44.3 0.3 0.69% 52.1 1.2 2.35%

C22:5n6 NA 5.54 1.17 21.13% <LOD 4.39 1.17 26.73% 14.5 0.3 2.35% 16.1 0.5 3.14%

C22:6n3 156 3 1.85% 64.3 5.4 8.45% 4.53 0.61 13.44% 52.5 3.3 6.27% 136 2 1.31% 179 1 0.33%

C24:0 58.3 2.5 4.23% 0.966 0.065 6.73% <LOD 20.0 3.6 17.87% 81.9 2.7 3.29% 78.0 1.0 1.33%

C24:1n9 61.6 3.4 5.44% <LOD 19.4 3.7 19.12% 84.4 1.7 1.99% 86.4 2.6 2.95%

NOTE for Lab 9:

C18:1n9 Oleic acid Total 18:1 determined & entered

C20:1n9 11-Eicosenoic acid Total 20:1 determined & entered

C22:1n9 Docosenoic acid Total 22:1 determined & entered
9

Table 3 (continued) Interlaboratory data received for SRM 2378-2

Lab 1 Lab 2 Lab 3 Lab 4 Lab 5

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

density (g/mL) 1.02 0.00 0.01% not reported 1.06 0.04 3.45% not reported 1.01 0.02 2.03%

CONVERT ALL TO μmol/L

Lab 1 Lab 2 Lab 3 Lab 4 Lab 5

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

C14:0 158 11 6.82% 158 1 0.45% 153 8 5.41% 126 8 6.13% 175 9 5.16%

C14:1n5 15.3 1.2 8.18% 19.3 0.6 2.99% NA 11.4 0.5 4.71%

C16:0 2870 90 3.13% 1523 32 2.11% 2811 122 4.35% 2762 142 5.15% 3680 102 2.78%

C16:1n7 288 8 2.72% 264 13 4.74% 262 16 5.99% 242 11 4.57% 333 8 2.46%

C18:0 886 26 2.88% 785 22 2.80% 864 29 3.41% 848 55 6.49% 1122 34 3.01%

C18:1n7 114 5 4.48% 264 4 1.33% 109 7 6.46% NA 222 3 1.32%

C18:1n9 2561 35 1.36% 1303 64 4.93% 2611 152 5.81% 2461 214 8.68% 3285 46 1.41%

C18:2n6 4355 185 4.24% 4067 64 1.58% 4378 255 5.83% 3615 415 11.48% 5328 72 1.35%

C18:3n3 114 4 3.77% 116 5 3.88% 96.6 6.8 7.04% 86.6 9.5 10.99% 130 2 1.25%

C18:3n6 74.8 3.6 4.82% 82.0 4.6 5.59% 71.3 7.7 10.81% 65.0 5.6 8.58% 93.9 1.2 1.24%

C20:0 23.1 1.2 5.20% 78.3 2.3 2.95% 28.7 3.8 13.15% 15.2 3.5 23.05% 23.9 2.1 8.93%

C20:1n9 16.2 0.8 4.63% NA 20.7 2.4 11.59% 12.3 1.2 9.45% 17.0 0.6 3.72%

C20:2n6 NA NA 24.5 1.8 7.40% 17.4 0.8 4.82% 25.8 0.5 1.90%

C20:3n6 NA 89.3 1.2 1.29% 200 11 5.42% 167 14 8.36% 231 2 0.84%

C20:4n6 757 27 3.61% 1330 56 4.19% 746 36 4.88% 668 72 10.74% 972 8 0.86%

C20:5n3 74.0 2.0 2.67% 115 3 2.79% 64.4 9.1 14.10% 76.2 6.3 8.28% 82.4 0.7 0.81%

C22:0 63.5 3.8 5.99% 59.2 0.3 0.54% 27.8 6.2 22.35% 33.9 5.2 15.32% 62.9 5.9 9.42%

C22:1n9 7.54 0.19 2.54% 7.00 0.00 0.00% NA NA 9.39 0.36 3.81%

C22:4n6 NA 17.7 0.6 3.27% 21.0 3.2 15.04% 17.8 2.3 12.94% 23.3 3.1 13.45%

C22:5n3 55.0 0.5 0.87% 45.3 4.0 8.91% 49.3 3.8 7.81% 39.0 1.7 4.30% 55.9 1.5 2.73%

C22:5n6 NA 9.67 1.53 15.80% 12.0 1.5 12.18% 13.4 0.7 4.84% 15.7 0.6 3.90%

C22:6n3 181 9 5.19% 184 13 7.11% 163 10 6.28% 145 2 1.43% 182 1 0.61%

C24:0 44.7 5.2 11.57% 60.2 1.5 2.45% 18.4 7.2 39.06% 21.2 0.9 4.42% 46.8 6.1 13.01%

C24:1n9 49.2 2.9 5.83% 67.3 2.1 3.09% 21.9 9.0 41.35% 20.4 6.0 29.67% 51.9 5.1 9.88%

Table 3 (continued) Interlaboratory data received for SRM 2378-2

Lab 6 Lab 7 Lab 8 Lab 9 Lab 10 Lab 11

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

density (g/mL) 1.04 0.00 0.14% 0.995 0.016 1.60% 1.00 0.00 0.01% not reported use 1 0.983 0.006 0.64%

CONVERT ALL TO μmol/L

Lab 6 Lab 7 Lab 8 Lab 9 Lab 10 Lab 11

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

C14:0 142 3 2.11% 152 15 9.60% 19.9 1.0 4.80% 150 5 3.20% 86.4 0.8 0.88% 152 1 0.63%

C14:1n5 NA 15.0 1.3 8.95% <LOD 12.1 0.8 6.86% 5.49 0.62 11.35% 9.24 0.16 1.68%

C16:0 2963 87 2.95% 2782 329 11.81% 299 16 5.24% 2995 122 4.07% 2057 15 0.72% 3151 20 0.62%

C16:1n7 266 7 2.56% 63.9 6.7 10.47% 8.08 0.81 10.07% 258 11 4.20% 182 3 1.80% 264 4 1.48%

C18:0 905 12 1.33% 997 94 9.47% 102 10 9.86% 928 44 4.69% 696 4 0.64% 808 3 0.38%

C18:1n7 146 0 0.00% 118 11 9.13% 36.2 2.4 6.55% NA 130 8 6.06% 140 1 0.90%

C18:1n9 2680 85 3.19% 2473 308 12.45% 127 7 5.79% 2927 103 3.51% 1984 23 1.17% 2764 23 0.84%

C18:2n6 4353 172 3.96% 3180 354 11.12% 100 6 6.44% 4354 147 3.38% 3200 40 1.24% 4479 37 0.81%

C18:3n3 108 2 1.85% 107 17 15.80% <LOD 112 6 5.36% 78.7 0.7 0.85% 118 1 1.25%

C18:3n6 74.3 1.2 1.62% 54.5 8.1 14.92% 5.47 0.29 5.26% 76.1 2.7 3.54% 54.4 1.1 2.07% 76.3 0.7 0.87%

C20:0 NA 25.0 3.6 14.25% <LOD 34.0 5.2 15.29% 26.1 0.4 1.64% 29.4 0.3 0.84%

C20:1n9 NA 37.3 12.2 32.85% <LOD 14.4 1.7 11.95% 12.2 0.4 3.13% 16.0 0.3 1.96%

C20:2n6 NA 5.98 1.65 27.55% <LOD 24.8 1.5 5.92% 19.4 0.5 2.82% 23.1 0.2 0.87%

C20:3n6 189 3 1.52% 209 23 11.06% 7.94 0.50 6.32% 190 11 5.62% 156 2 1.25% 196 7 3.35%

C20:4n6 823 17 2.07% 780 85 10.89% 33.9 1.8 5.20% 813 49 6.03% 646 9 1.42% 856 8 0.94%

C20:5n3 68.5 1.1 1.55% 68.0 6.1 8.95% <LOD 67.4 5.1 7.59% 62.6 0.2 0.30% 71.9 0.5 0.73%

C22:0 69.7 1.9 2.66% 2.37 0.21 8.89% <LOD 66.7 11.0 16.55% 88.6 1.6 1.85% 92.7 1.2 1.30%

C22:1n9 NA <LOD 16.3 3.3 20.05% 2.20 0.31 14.02% 4.46 0.32 7.19%

C22:4n6 21.3 0.8 3.79% 27.3 2.5 9.24% <LOD 41.8 2.2 5.23% 20.2 0.5 2.55% 23.0 1.3 5.81%

C22:5n3 48.3 1.1 2.28% 53.5 8.1 15.16% <LOD 46.4 2.8 6.03% 44.3 0.3 0.69% 52.1 1.2 2.35%

C22:5n6 NA 16.7 3.5 21.13% <LOD 13.3 3.6 26.73% 14.5 0.3 2.35% 16.1 0.5 3.14%

C22:6n3 156 3 1.85% 195 16 8.45% 4.53 0.61 13.44% 160 10 6.27% 136 2 1.31% 179 1 0.33%

C24:0 58.3 2.5 4.23% 2.61 0.18 6.73% <LOD 54.3 9.7 17.87% 81.9 2.7 3.29% 78.0 1.0 1.33%

C24:1n9 61.6 3.4 5.44% <LOD 52.9 10.1 19.12% 84.4 1.7 1.99% 86.4 2.6 2.95%

NOTE for Lab 9:

C18:1n9 Oleic acid Total 18:1 determined & entered

C20:1n9 11-Eicosenoic acid Total 20:1 determined & entered

C22:1n9 Docosenoic acid Total 22:1 determined & entered
10

Table 4. Interlaboratory data received for SRM 2378-3

units reported μg/g μmol/L μmol/L μmol/L μg/g

Lab 1 Lab 2 Lab 3 Lab 4 Lab 5

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

C14:0 34.1 2.0 5.75% 158 3 1.60% 163 2 1.52% 129 6 4.58% 40.1 0.7 1.84%

C14:1n5 4.02 0.29 7.31% 19.3 0.6 2.99% NA 2.81 0.18 6.41%

C16:0 774 11 1.47% 1307 29 2.21% 2401 28 1.16% 2495 141 5.66% 780 7 0.83%

C16:1n7 49.4 1.7 3.51% 174 8 4.83% 180 2 1.13% 175 5 3.02% 56.7 0.5 0.79%

C18:0 207 10 4.88% 663 10 1.44% 742 8 1.08% 736 31 4.26% 265 2 0.87%

C18:1n7 36.3 3.2 8.94% 212 5 2.33% 92.5 2.4 2.59% NA 51.3 0.7 1.36%

C18:1n9 603 16 2.57% 1077 61 5.68% 1841 26 1.43% 1769 108 6.12% 649 7 1.11%

C18:2n6 931 27 2.91% 3327 107 3.21% 3282 53 1.63% 2689 200 7.44% 1103 6 0.53%

C18:3n3 16.9 0.2 1.36% 61.0 1.7 2.84% 51.6 1.1 2.20% 40.8 2.4 5.89% 19.3 0.4 1.87%

C18:3n6 15.1 0.4 2.41% 50.3 0.6 1.15% 46.6 1.8 3.86% 41.9 1.9 4.44% 17.8 0.4 2.24%

C20:0 10.1 0.2 1.57% 59.0 1.0 1.69% 30.6 4.1 13.29% 12.5 1.8 14.17% 6.06 0.27 4.41%

C20:1n9 5.49 0.09 1.64% NA 20.7 1.4 6.73% 14.4 1.9 13.24% 5.62 0.30 5.34%

C20:2n6 NA NA 20.5 0.4 2.11% 16.6 1.8 10.98% 6.79 0.12 1.77%

C20:3n6 NA 62.0 1.0 1.61% 126 2 1.31% 102 7 6.42% 43.5 0.6 1.47%

C20:4n6 245 12 5.10% 1127 12 1.02% 691 13 1.89% 596 36 5.97% 264 2 0.76%

C20:5n3 21.0 0.5 2.61% 87.0 7.2 8.29% 59.8 3.2 5.40% 61.4 2.2 3.63% 21.5 0.5 2.10%

C22:0 14.8 0.3 2.02% 42.2 1.0 2.34% 20.7 5.0 24.11% 28.0 2.2 7.97% 14.6 0.9 5.83%

C22:1n9 2.83 0.07 2.36% 6.00 0.00 0.00% NA NA 3.07 0.07 2.28%

C22:4n6 NA 12.3 0.6 4.68% 19.7 2.7 13.82% 13.1 0.5 3.62% 4.51 0.46 10.08%

C22:5n3 10.5 0.1 0.63% 28.7 0.6 2.01% 36.7 1.3 3.67% 27.0 2.1 7.96% 12.7 1.0 8.19%

C22:5n6 NA 8.67 0.58 6.66% 12.7 2.6 20.27% 11.5 2.4 20.79% 4.84 0.05 1.05%

C22:6n3 51.1 0.6 1.13% 157 7 4.18% 173 2 1.03% 124 3 2.04% 59.9 0.4 0.70%

C24:0 12.5 0.1 0.70% 43.4 1.0 2.32% 16.5 3.4 20.60% 16.3 1.2 7.33% 11.0 0.6 5.55%

C24:1n9 13.6 0.9 6.60% 48.3 2.1 4.31% 15.9 1.0 6.39% 13.1 1.9 14.58% 14.2 0.4 2.82%

Table 4 (continued) Interlaboratory data received for SRM 2378-3

units reported μmol/L μg/g μmol/L μg/g μmol/L μmol/L

Lab 6 Lab 7 Lab 8 Lab 9 Lab 10 Lab 11

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

C14:0 149 3 1.69% 51.7 1.5 2.98% 22.9 1.9 8.24% 35.8 2.3 6.36% 88.2 1.2 1.40% 157 2 1.12%

C14:1n5 NA 5.62 0.72 12.73% <LOD 2.82 0.09 3.14% 5.69 0.28 4.97% 10.4 0.1 0.79%

C16:0 2473 67 2.69% 827 18 2.22% 300 19 6.22% 644 27 4.27% 1719 15 0.85% 2592 23 0.88%

C16:1n7 181 5 2.49% 15.1 0.5 3.44% 8.23 1.02 12.35% 44.7 1.9 4.36% 120 3 2.30% 177 0 0.27%

C18:0 781 10 1.26% 308 5 1.48% 93.6 12.5 13.32% 221 11 4.75% 587 7 1.24% 657 12 1.81%

C18:1n7 118 2 1.69% 34.9 12.6 36.00% 39.6 2.7 6.81% ND 107 4 3.77% 110 1 0.95%

C18:1n9 1927 40 2.10% 744 21 2.85% 101 7 7.13% 585 31 5.31% 1386 4 0.31% 1938 16 0.82%

C18:2n6 3343 121 3.61% 1128 50 4.43% 115 9 7.94% 906 47 5.16% 2331 16 0.68% 3328 21 0.64%

C18:3n3 57.8 1.4 2.40% 23.9 1.6 6.88% <LOD 17.4 0.6 3.49% 41.3 0.2 0.59% 62.6 0.6 0.93%

C18:3n6 50.3 1.6 3.08% 18.7 0.8 4.16% <LOD 14.4 0.9 6.25% 34.0 0.1 0.38% 51.3 0.7 1.35%

C20:0 NA 10.3 0.5 4.93% <LOD 8.64 1.80 20.81% 19.2 0.4 1.88% 21.2 0.4 1.87%

C20:1n9 NA 9.16 3.54 38.65% <LOD 4.10 0.07 1.59% 13.1 0.3 2.02% 16.8 0.1 0.57%

C20:2n6 NA 2.06 0.43 20.84% <LOD 6.62 0.31 4.72% 16.0 0.3 2.00% 19.6 0.1 0.61%

C20:3n6 117 2 1.31% 54.6 1.5 2.67% 5.04 0.29 5.73% 36 1 3.78% 95.4 1.2 1.31% 121 0 0.29%

C20:4n6 799 17 2.07% 322 11 3.45% 32.1 2.0 6.10% 222 11 4.81% 569 3 0.60% 768 7 0.92%

C20:5n3 62.9 1.0 1.66% 26.6 0.6 2.06% <LOD 18.1 0.7 4.05% 50.8 0.7 1.35% 62.2 1.0 1.65%

C22:0 47.0 1.1 2.37% 1.22 0.64 52.34% <LOD 15.9 0.2 1.25% 59.8 0.9 1.55% 61.0 0.2 0.29%

C22:1n9 NA <LOD 6.74 1.07 15.83% 2.09 0.08 3.79% 5.03 0.51 10.20%

C22:4n6 17.0 0.2 1.36% 9.91 0.10 0.96% <LOD 10.8 1.2 10.78% 16.1 0.2 1.17% 18.6 0.3 1.78%

C22:5n3 33.1 0.7 2.09% 16.9 0.9 5.35% <LOD 10.24 0.36 3.48% 29.3 0.6 2.05% 36.4 1.1 2.98%

C22:5n6 NA 7.06 0.41 5.78% <LOD 4.57 0.55 12.02% 13.8 0.5 3.48% 16.0 0.5 3.28%

C22:6n3 157 3 1.69% 83.6 3.2 3.85% 5.89 0.35 5.89% 51.6 2.7 5.19% 134 0 0.35% 177 2 1.27%

C24:0 39.5 0.4 1.10% 1.53 0.28 18.51% <LOD 15.2 0.8 5.03% 56.5 1.8 3.13% 53.2 0.3 0.58%

C24:1n9 43.6 1.8 4.05% <LOD 15.4 0.3 1.90% 63.8 1.3 2.05% 63.5 1.1 1.68%

NOTE for Lab 9:

C18:1n9 Oleic acid Total 18:1 determined & entered

C20:1n9 11-Eicosenoic acid Total 20:1 determined & entered

C22:1n9 Docosenoic acid Total 22:1 determined & entered
11

Table 4 (continued) Interlaboratory data received for SRM 2378-3

Lab 1 Lab 2 Lab 3 Lab 4 Lab 5

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

density (g/mL) 1.02 0.00 0.00% not reported 1.08 0.02 1.86% not reported 1.02 0.01 0.56%

CONVERT ALL TO μmol/L

Lab 1 Lab 2 Lab 3 Lab 4 Lab 5

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

C14:0 152 9 5.75% 158 3 1.60% 163 2 1.52% 129 6 4.58% 180 3 1.84%

C14:1n5 18.2 1.3 7.31% 19.3 0.6 2.99% NA 12.7 0.8 6.41%

C16:0 3085 45 1.47% 1307 29 2.21% 2401 28 1.16% 2495 141 5.66% 3113 26 0.83%

C16:1n7 199 7 3.51% 174 8 4.83% 180 2 1.13% 175 5 3.02% 228 2 0.79%

C18:0 745 36 4.88% 663 10 1.44% 742 8 1.08% 736 31 4.26% 953 8 0.87%

C18:1n7 131 12 8.94% 212 5 2.33% 92.5 2.4 2.59% NA 186 3 1.36%

C18:1n9 2183 56 2.57% 1077 61 5.68% 1841 26 1.43% 1769 108 6.12% 2351 26 1.11%

C18:2n6 3393 99 2.91% 3327 107 3.21% 3282 53 1.63% 2689 200 7.44% 4024 21 0.53%

C18:3n3 62.2 0.8 1.36% 61.0 1.7 2.84% 51.6 1.1 2.20% 40.8 2.4 5.89% 70.9 1.3 1.87%

C18:3n6 55.5 1.3 2.41% 50.3 0.6 1.15% 46.6 1.8 3.86% 41.9 1.9 4.44% 65.5 1.5 2.24%

C20:0 33.1 0.5 1.57% 59.0 1.0 1.69% 30.6 4.1 13.29% 12.5 1.8 14.17% 19.8 0.9 4.41%

C20:1n9 18.1 0.3 1.64% NA 20.7 1.4 6.73% 14.4 1.9 13.24% 18.5 1.0 5.34%

C20:2n6 NA NA 20.5 0.4 2.11% 16.6 1.8 10.98% 22.5 0.4 1.77%

C20:3n6 NA 62.0 1.0 1.61% 126 2 1.31% 102 7 6.42% 145 2 1.47%

C20:4n6 822 42 5.10% 1127 12 1.02% 691 13 1.89% 596 36 5.97% 887 7 0.76%

C20:5n3 70.9 1.8 2.61% 87.0 7.2 8.29% 59.8 3.2 5.40% 61.4 2.2 3.63% 72.6 1.5 2.10%

C22:0 44.4 0.9 2.02% 42.2 1.0 2.34% 20.7 5.0 24.11% 28.0 2.2 7.97% 43.8 2.6 5.83%

C22:1n9 8.53 0.20 2.36% 6.00 0.00 0.00% NA NA 9.28 0.21 2.28%

C22:4n6 NA 12.3 0.6 4.68% 19.7 2.7 13.82% 13.1 0.5 3.62% 13.9 1.4 10.08%

C22:5n3 32.3 0.2 0.63% 28.7 0.6 2.01% 36.7 1.3 3.67% 27.0 2.1 7.96% 39.3 3.2 8.19%

C22:5n6 NA 8.67 0.58 6.66% 12.7 2.6 20.27% 11.5 2.4 20.79% 15.0 0.2 1.05%

C22:6n3 159 2 1.13% 157 7 4.18% 173 2 1.03% 124 3 2.04% 186 1 0.70%

C24:0 34.7 0.2 0.70% 43.4 1.0 2.32% 16.5 3.4 20.60% 16.3 1.2 7.33% 30.5 1.7 5.55%

C24:1n9 37.9 2.5 6.60% 48.3 2.1 4.31% 15.9 1.0 6.39% 13.1 1.9 14.58% 39.5 1.1 2.82%

Table 4 (continued) Interlaboratory data received for SRM 2378-3

Lab 6 Lab 7 Lab 8 Lab 9 Lab 10 Lab 11

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

density (g/mL) 1.04 0.00 0.47% 0.988 0.010 1.00% 1.02 0.00 0.01% not reported use 1 0.987 0.001 0.06%

CONVERT ALL TO μmol/L

Lab 6 Lab 7 Lab 8 Lab 9 Lab 10 Lab 11

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

C14:0 149 3 1.69% 224 7 2.98% 22.9 1.9 8.24% 157 10 6.36% 88.2 1.2 1.40% 157 2 1.12%

C14:1n5 NA 24.5 3.1 12.73% <LOD 12.4 0.4 3.14% 5.69 0.28 4.97% 10.4 0.1 0.79%

C16:0 2473 67 2.69% 3186 71 2.22% 300 19 6.22% 2511 107 4.27% 1719 15 0.85% 2592 23 0.88%

C16:1n7 181 5 2.49% 58.6 2.0 3.44% 8.23 1.02 12.35% 176 8 4.36% 120 3 2.30% 177 0 0.27%

C18:0 781 10 1.26% 1069 16 1.48% 93.6 12.5 13.32% 777 37 4.75% 587 7 1.24% 657 12 1.81%

C18:1n7 118 2 1.69% 122 44 36.00% 39.6 2.7 6.81% NA 107 4 3.77% 110 1 0.95%

C18:1n9 1927 40 2.10% 2603 74 2.85% 101 7 7.13% 2071 110 5.31% 1386 4 0.31% 1938 16 0.82%

C18:2n6 3343 121 3.61% 3973 176 4.43% 115 9 7.94% 3231 167 5.16% 2331 16 0.68% 3328 21 0.64%

C18:3n3 57.8 1.4 2.40% 84.6 5.8 6.88% <LOD 62.6 2.2 3.49% 41.3 0.2 0.59% 62.6 0.6 0.93%

C18:3n6 50.3 1.6 3.08% 66.2 2.8 4.16% <LOD 51.7 3.2 6.25% 34.0 0.1 0.38% 51.3 0.7 1.35%

C20:0 NA 32.4 1.6 4.93% <LOD 27.6 5.8 20.81% 19.2 0.4 1.88% 21.2 0.4 1.87%

C20:1n9 NA 29.1 11.3 38.65% <LOD 13.2 0.2 1.59% 13.1 0.3 2.02% 16.8 0.1 0.57%

C20:2n6 NA 6.59 1.37 20.84% <LOD 21.5 1.0 4.72% 16.0 0.3 2.00% 19.6 0.1 0.61%

C20:3n6 117 2 1.31% 176 5 2.67% 5.04 0.29 5.73% 116 4 3.78% 95.4 1.2 1.31% 121 0 0.29%

C20:4n6 799 17 2.07% 1045 36 3.45% 32.1 2.0 6.10% 728 35 4.81% 569 3 0.60% 768 7 0.92%

C20:5n3 62.9 1.0 1.66% 87.00 1.80 2.06% <LOD 59.7 2.4 4.05% 50.8 0.7 1.35% 62.2 1.0 1.65%

C22:0 47.0 1.1 2.37% 3.53 1.85 52.34% <LOD 46.7 0.6 1.25% 59.8 0.9 1.55% 61.0 0.2 0.29%

C22:1n9 NA <LOD 19.9 3.1 15.83% 2.09 0.08 3.79% 5.03 0.51 10.20%

C22:4n6 17.0 0.2 1.36% 29.4 0.3 0.96% <LOD 32.6 3.5 10.78% 16.1 0.2 1.17% 18.6 0.3 1.78%

C22:5n3 33.1 0.7 2.09% 50.6 2.7 5.35% <LOD 31.0 1.1 3.48% 29.3 0.6 2.05% 36.4 1.1 2.98%

C22:5n6 NA 21.1 1.2 5.78% <LOD 13.8 1.7 12.02% 13.8 0.5 3.48% 16.0 0.5 3.28%

C22:6n3 157 3 1.69% 251 10 3.85% 5.89 0.35 5.89% 157 8 5.19% 134 0 0.35% 177 2 1.27%

C24:0 39.5 0.4 1.10% 4.10 0.76 18.51% <LOD 41.2 2.1 5.03% 56.5 1.8 3.13% 53.2 0.3 0.58%

C24:1n9 43.6 1.8 4.05% <LOD 41.9 0.8 1.90% 63.8 1.3 2.05% 63.5 1.1 1.68%

NOTE for Lab 9:

C18:1n9 Oleic acid Total 18:1 determined & entered

C20:1n9 11-Eicosenoic acid Total 20:1 determined & entered

C22:1n9 Docosenoic acid Total 22:1 determined & entered
12

Table 5. Interlaboratory data received for SRM 1950

units reported μmol/L μg/g μmol/L μmol/L μmol/L μg/g

COA Lab 1 Lab 2 Lab 3 Lab 4 Lab 5

Cert/ref unc rel unc mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

C14:0 80.1 17 21.22% 26.2 0.3 1.02% 115 4 3.06% 111 4 3.50% 98.7 2.2 2.25% 26.7 0.4 1.35%

C14:1n5 7.1 0.1 1.41% 1.90 0.03 1.69% 16.3 0.6 3.53% NA 1.80 0.08 4.17%

C16:0 2364 77 3.26% 624 14 2.28% 1273 25 1.98% 2210 105 4.73% 2532 70 2.77% 737 7 0.90%

C16:1n7 215 26 12.09% 56.1 0.8 1.43% 252 2 0.69% 271 13 4.98% 271 2 0.58% 84.8 1.0 1.12%

C18:0 644 41 6.37% 174 8 4.36% 612 16 2.59% 652 45 6.91% 707 23 3.20% 237 2 0.88%

C18:1n7 136 3 2.21% 35.6 0.9 2.39% 295 4 1.22% 131 7 5.05% NA 64.8 0.6 0.94%

C18:1n9 1614 154 9.54% 451 12 2.59% 1113 21 1.87% 1717 63 3.70% 1868 40 2.13% 643 5 0.80%

C18:2n6 2838 143 5.04% 879 9 1.08% 3030 98 3.25% 2806 104 3.69% 2607 110 4.22% 991 10 0.98%

C18:3n3 54.6 3.6 6.59% 16.8 0.4 2.63% 52.7 0.6 1.10% 44.7 1.2 2.68% 43.2 0.9 2.07% 17.2 0.3 1.86%

C18:3n6 39.9 8.5 21.30% 13.4 0.1 0.97% 55.3 1.5 2.76% 46.5 2.6 5.49% 47.5 2.8 5.93% 18.1 0.1 0.32%

C20:0 18.0 0.5 2.78% 5.57 0.05 0.81% 51.7 1.2 2.23% 21.1 4.2 19.70% 11.5 1.2 10.42% 4.44 0.50 11.26%

C20:1n9 11.5 0.5 4.35% 3.44 0.19 5.57% NA 15.5 4.4 28.53% 10.3 0.9 8.85% 4.14 0.21 5.07%

C20:2n6 18.8 0.6 3.19% NA NA 19.1 1.3 6.65% 18.8 1.1 6.09% 6.82 0.07 1.03%

C20:3n6 139 4 2.88% NA 68.0 2.0 2.94% 141 4 2.78% 124 2 1.49% 50.3 0.7 1.33%

C20:4n6 984 180 18.29% 274 13 4.71% 1067 15 1.43% 627 23 3.74% 625 10 1.59% 253 3 0.99%

C20:5n3 38.6 0.5 1.30% 12.3 0.5 4.01% 60.7 2.1 3.43% 37.4 2.1 5.56% 53.0 6.1 11.59% 14.3 0.3 1.82%

C22:0 47.8 6.0 12.55% 12.3 0.4 3.01% 37.1 0.6 1.48% 16.6 4.7 28.07% 30.4 3.8 12.56% 11.1 1.2 10.48%

C22:1n9 3.4 1.3 38.24% 1.79 0.08 4.49% 8.00 0.00 0.00% NA NA 2.27 0.36 15.86%

C22:4n6 25.5 0.6 2.35% NA 17.3 0.6 3.33% 25.7 3.5 13.53% 20.7 1.3 6.20% 4.17 0.17 4.08%

C22:5n3 38.5 0.7 1.82% 12.5 0.3 2.78% 32.7 1.2 3.53% 38.7 4.4 11.35% 33.4 2.2 6.72% 15.1 0.2 0.99%

C22:5n6 19.5 0.4 2.05% NA 11.7 0.6 4.95% 16.5 2.1 12.90% 18.4 0.9 4.82% 6.74 0.20 2.97%

C22:6n3 118 21 17.80% 38.1 0.9 2.24% 94.3 2.9 3.06% 109 5 4.58% 89.8 2.0 2.26% 37.3 0.2 0.46%

C24:0 46.6 2.6 5.58% 15.7 0.3 1.63% 38.0 1.1 2.77% 10.9 4.0 36.37% 13.0 1.5 11.21% 10.2 0.7 6.55%

C24:1n9 71.3 3.2 4.49% 17.1 0.3 1.64% 51.7 3.1 5.91% 16.0 3.4 20.96% 19.3 3.8 19.64% 14.7 1.4 9.82%

Table 5 (Continued) Interlaboratory data received for SRM 1950

units reported μmol/L μg/g μmol/L μg/g μmol/L μmol/L

Lab 6 Lab 7 Lab 8 Lab 9 Lab 10 Lab 11

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

C14:0 103 1 0.97% 37.3 3.9 10.45% 11.9 0.5 4.56% 25.0 0.3 1.06% 61.4 5.0 8.14% 112 3 2.36%

C14:1n5 NA 3.77 0.06 1.64% <LOD 2.22 0.12 5.37% 4.31 0.66 15.24% 7.46 0.14 1.85%

C16:0 2343 58 2.46% 793 58 7.31% 341 6 1.87% 612 8 1.33% 1643 53 3.22% 2571 71 2.76%

C16:1n7 272 3 1.10% 15.9 0.7 4.26% 12.4 2.0 16.47% 75.2 1.9 2.58% 184 10 5.26% 277 6 2.17%

C18:0 679 8 1.11% 268 16 5.90% 113 4 3.31% 205 1 0.27% 526 6 1.21% 609 19 3.15%

C18:1n7 133 4 3.12% 54.0 14.0 25.86% 45.4 1.7 3.76% ND 134 10 7.62% 131 3 2.61%

C18:1n9 1837 47 2.57% 718 65 9.03% 116 5 4.50% 608 3 0.46% 1363 80 5.90% 1906 38 2.01%

C18:2n6 2897 110 3.79% 1071 112 10.46% 134 5 3.96% 822 0 0.02% 2081 112 5.39% 3066 66 2.15%

C18:3n3 53.3 1.8 3.31% 21.5 1.9 8.99% <LOD 15.6 0.1 0.82% 36.6 2.1 5.84% 57.6 1.0 1.67%

C18:3n6 52.5 1.8 3.37% 20.5 2.4 11.44% 4.48 0.52 11.62% 15.0 0.7 4.96% 36.6 2.8 7.66% 54.3 0.7 1.36%

C20:0 NA 11.7 0.7 6.11% <LOD 7.97 0.45 5.61% 16.1 0.2 1.41% 18.1 0.5 2.74%

C20:1n9 NA 8.62 4.32 50.13% <LOD 4.02 0.38 9.54% 10.5 1.0 9.56% 12.4 0.2 1.29%

C20:2n6 NA 2.43 0.27 11.00% <LOQ 6.15 0.34 5.53% 15.9 0.2 1.04% 19.4 0.3 1.66%

C20:3n6 139 2 1.25% 63.2 3.7 5.84% 12.0 0.7 6.14% 41.9 0.3 0.83% 111 1 1.27% 146 2 1.45%

C20:4n6 704 14 2.04% 314 24 7.59% 57.4 1.0 1.68% 212 2 0.71% 540 16 2.97% 752 18 2.37%

C20:5n3 42.0 1.3 3.10% 18.9 1.8 9.59% <LOD 12.3 0.5 4.34% 35.8 1.4 3.90% 42.2 2.0 4.72%

C22:0 37.5 0.6 1.61% 1.02 0.14 13.86% <LOD 13.4 1.1 8.31% 49.1 0.5 1.10% 52.1 1.2 2.32%

C22:1n9 NA <LOD 7.01 0.81 11.49% 2.12 0.11 5.03% 4.75 0.47 9.81%

C22:4n6 24.1 0.5 1.87% 13.9 1.0 7.46% 1.67 0.06 3.88% 13.0 0.6 4.39% 22.4 0.6 2.50% 28.1 1.1 4.07%

C22:5n3 38.3 0.8 1.96% 19.9 0.8 4.08% 2.97 0.27 8.94% 12.7 0.1 0.55% 34.6 0.2 0.68% 42.2 1.5 3.54%

C22:5n6 NA 9.14 0.44 4.81% <LOD 5.73 0.60 10.45% 18.0 1.3 7.21% 21.5 0.4 1.73%

C22:6n3 95.8 2.9 3.02% 56.4 4.6 8.14% 8.12 0.66 8.17% 32.8 1.3 4.00% 82.3 0.5 0.57% 113 3 2.66%

C24:0 32.1 0.7 2.08% 0.921 0.168 18.24% <LOD 12.1 1.3 10.38% 47.3 0.2 0.48% 45.5 1.0 2.25%

C24:1n9 48.2 1.2 2.45% <LOD 16.4 1.2 7.44% 72.1 3.1 4.32% 71.4 1.9 2.62%

NOTE for Lab 9:

C18:1n9 Oleic acid Total 18:1 determined & entered

C20:1n9 11-Eicosenoic acid Total 20:1 determined & entered

C22:1n9 Docosenoic acid Total 22:1 determined & entered
13

Table 5 (Continued) Interlaboratory data received for SRM 1950

Lab 1 Lab 2 Lab 3 Lab 4 Lab 5

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

density (g/mL) 1.02 0.00 0.00 not reported 1.04 0.02 1.78% not reported 1.01 0.02 2.11%

CONVERT ALL TO μmol/L

COA Lab 1 Lab 2 Lab 3 Lab 4 Lab 5

Cert/ref unc rel unc mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

C14:0 80.1 17 21.22% 117 1 1.02% 115 4 3.06% 111 4 3.50% 98.7 2.2 2.25% 118 2 1.35%

C14:1n5 7.1 0.1 1.41% 8.55 0.14 1.69% 16.3 0.6 3.53% NA 8.04 0.34 4.17%

C16:0 2364 77 3.26% 2483 56 2.28% 1273 25 1.98% 2210 105 4.73% 2532 70 2.77% 2911 26 0.90%

C16:1n7 215 26 12.09% 225 3 1.43% 252 2 0.69% 271 13 4.98% 271 2 0.58% 338 4 1.12%

C18:0 644 41 6.37% 623 27 4.36% 612 16 2.59% 652 45 6.91% 707 23 3.20% 844 7 0.88%

C18:1n7 136 3 2.21% 129 3 2.39% 295 4 1.22% 131 7 5.05% NA 232 2 0.94%

C18:1n9 1614 154 9.54% 1629 42 2.59% 1113 21 1.87% 1717 63 3.70% 1868 40 2.13% 2305 18 0.80%

C18:2n6 2838 143 5.04% 3198 34 1.08% 3030 98 3.25% 2806 104 3.69% 2607 110 4.22% 3578 35 0.98%

C18:3n3 54.6 3.6 6.59% 61.5 1.6 2.63% 52.7 0.6 1.10% 44.7 1.2 2.68% 43.2 0.9 2.07% 62.5 1.2 1.86%

C18:3n6 39.9 8.5 21.30% 49.2 0.5 0.97% 55.3 1.5 2.76% 46.5 2.6 5.49% 47.5 2.8 5.93% 65.7 0.2 0.32%

C20:0 18.0 0.5 2.78% 18.2 0.1 0.81% 51.7 1.2 2.23% 21.1 4.2 19.70% 11.5 1.2 10.42% 14.4 1.6 11.26%

C20:1n9 11.5 0.5 4.35% 11.3 0.6 5.57% NA 15.5 4.4 28.53% 10.3 0.9 8.85% 13.5 0.7 5.07%

C20:2n6 18.8 0.6 3.19% NA NA 19.1 1.3 6.65% 18.8 1.1 6.09% 22.4 0.2 1.03%

C20:3n6 139 4 2.88% NA 68.0 2.0 2.94% 141 4 2.78% 124 2 1.49% 166 2 1.33%

C20:4n6 984 180 18.29% 917 43 4.71% 1067 15 1.43% 627 23 3.74% 625 10 1.59% 842 8 0.99%

C20:5n3 38.6 0.5 1.30% 41.5 1.7 4.01% 60.7 2.1 3.43% 37.4 2.1 5.56% 53.0 6.1 11.59% 47.9 0.9 1.82%

C22:0 47.8 6.0 12.55% 36.7 1.1 3.01% 37.1 0.6 1.48% 16.6 4.7 28.07% 30.4 3.8 12.56% 32.9 3.5 10.48%

C22:1n9 3.4 1.3 38.24% 5.39 0.24 4.49% 8.00 0.00 0.00% NA NA 6.79 1.08 15.86%

C22:4n6 25.5 0.6 2.35% NA 17.3 0.6 3.33% 25.7 3.5 13.53% 20.7 1.3 6.20% 12.7 0.5 4.08%

C22:5n3 38.5 0.7 1.82% 38.5 1.1 2.78% 32.7 1.2 3.53% 38.7 4.4 11.35% 33.4 2.2 6.72% 46.3 0.5 0.99%

C22:5n6 19.5 0.4 2.05% NA 11.7 0.6 4.95% 16.5 2.1 12.90% 18.4 0.9 4.82% 20.7 0.6 2.97%

C22:6n3 118 21 17.80% 118 3 2.24% 94.3 2.9 3.06% 109 5 4.58% 89.8 2.0 2.26% 115 1 0.46%

C24:0 46.6 2.6 5.58% 43.6 0.7 1.63% 38.0 1.1 2.77% 10.9 4.0 36.37% 13.0 1.5 11.21% 27.9 1.8 6.55%

C24:1n9 71.3 3.2 4.49% 47.7 0.8 1.64% 51.7 3.1 5.91% 16.0 3.4 20.96% 19.3 3.8 19.64% 40.5 4.0 9.82%

Table 5 (Continued) Interlaboratory data received for SRM 1950

Lab 6 Lab 7 Lab 8 Lab 9 Lab 10 Lab 11

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

density (g/mL) 1.04 0.00 0.21% 0.989 0.016 1.59% 0.997 0.000 0.01% not reported use 1 0.973 0.008 0.86%

CONVERT ALL TO μmol/L

Lab 6 Lab 7 Lab 8 Lab 9 Lab 10 Lab 11

mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

C14:0 103 1 0.97% 162 17 10.45% 11.9 0.5 4.56% 109 1 1.06% 61.4 5.0 8.14% 112 3 2.36%

C14:1n5 NA 16.5 0.3 1.64% <LOD 9.8 0.5 5.37% 4.31 0.66 15.24% 7.46 0.14 1.85%

C16:0 2343 58 2.46% 3058 224 7.31% 341 6 1.87% 2388 32 1.33% 1643 53 3.22% 2571 71 2.76%

C16:1n7 272 3 1.10% 61.6 2.6 4.26% 12.4 2.0 16.47% 296 8 2.58% 184 10 5.26% 277 6 2.17%

C18:0 679 8 1.11% 932 55 5.90% 113 4 3.31% 722 2 0.27% 526 6 1.21% 609 19 3.15%

C18:1n7 133 4 3.12% 189 49 25.86% 45.4 1.7 3.76% NA 134 10 7.62% 131 3 2.61%

C18:1n9 1837 47 2.57% 2513 227 9.03% 116 5 4.50% 2152 10 0.46% 1363 80 5.90% 1906 38 2.01%

C18:2n6 2897 110 3.79% 3776 395 10.46% 134 5 3.96% 2932 1 0.02% 2081 112 5.39% 3066 66 2.15%

C18:3n3 53.3 1.8 3.31% 76.2 6.9 8.99% <LOD 56.2 0.5 0.82% 36.6 2.1 5.84% 57.6 1.0 1.67%

C18:3n6 52.5 1.8 3.37% 72.9 8.3 11.44% 4.48 0.52 11.62% 53.9 2.7 4.96% 36.6 2.8 7.66% 54.3 0.7 1.36%

C20:0 NA 36.9 2.3 6.11% <LOD 25.5 1.4 5.61% 16.1 0.2 1.41% 18.1 0.5 2.74%

C20:1n9 NA 27.4 13.8 50.13% <LOD 12.9 1.2 9.54% 10.5 1.0 9.56% 12.4 0.2 1.29%

C20:2n6 NA 7.78 0.86 11.00% <LOQ 19.9 1.1 5.53% 15.9 0.2 1.04% 19.4 0.3 1.66%

C20:3n6 139 2 1.25% 204 12 5.84% 12.0 0.7 6.14% 137 1 0.83% 111 1 1.27% 146 2 1.45%

C20:4n6 704 14 2.04% 1019 77 7.59% 57.4 1.0 1.68% 696 5 0.71% 540 16 2.97% 752 18 2.37%

C20:5n3 42.0 1.3 3.10% 61.7 5.9 9.59% <LOD 40.6 1.8 4.34% 35.8 1.4 3.90% 42.2 2.0 4.72%

C22:0 37.5 0.6 1.61% 2.95 0.41 13.86% <LOD 39.4 3.3 8.31% 49.1 0.5 1.10% 52.1 1.2 2.32%

C22:1n9 NA <LOD 20.7 2.4 11.49% 2.12 0.11 5.03% 4.75 0.47 9.81%

C22:4n6 24.1 0.5 1.87% 41.2 3.1 7.46% 1.67 0.06 3.88% 38.9 1.7 4.39% 22.4 0.6 2.50% 28.1 1.1 4.07%

C22:5n3 38.3 0.8 1.96% 59.5 2.4 4.08% 2.97 0.27 8.94% 38.3 0.2 0.55% 34.6 0.2 0.68% 42.2 1.5 3.54%

C22:5n6 NA 27.3 1.3 4.81% <LOD 17.3 1.8 10.45% 18.0 1.3 7.21% 21.5 0.4 1.73%

C22:6n3 95.8 2.9 3.02% 170 14 8.14% 8.12 0.66 8.17% 100 4 4.00% 82.3 0.5 0.57% 113 3 2.66%

C24:0 32.1 0.7 2.08% 2.47 0.45 18.24% <LOD 33.0 3.4 10.38% 47.3 0.2 0.48% 45.5 1.0 2.25%

C24:1n9 48.2 1.2 2.45% <LOD 44.7 3.3 7.44% 72.1 3.1 4.32% 71.4 1.9 2.62%

NOTE for Lab 9:

C18:1n9 Oleic acid Total 18:1 determined & entered

C20:1n9 11-Eicosenoic acid Total 20:1 determined & entered

C22:1n9 Docosenoic acid Total 22:1 determined & entered
14

Table 6. Additional Data (μmol/L) Reported 

Lab Candidate SRM 2378-1 Candidate SRM 2378-2 Candidate SRM 2378-3 SRM 1950

reporting mean stdev rsd mean stdev rsd mean stdev rsd mean stdev rsd

11 C8:0 Caprylic (octanoic) 7.72 1.30 16.85% 1.51 0.87 57.50% 1.68 1.65 98.42% 2.80 4.53 161.54%

11 C10:0 Capric (decanoic) 21.1 0.1 0.41% 1.89 0.34 17.75% 5.27 0.37 6.96% 2.70 0.30 11.01%

7 C12:0 57.4 2.4 4.15% 22.8 2.2 9.81% 52.3 1.5 2.90% 26.9 4.0 14.76%

11 C12:0 Lauric (dodecanoic) 27.4 0.3 1.24% 11.3 0.4 3.76% 28.1 0.3 1.23% 9.27 0.04 0.47%

7 C15:0 41.4 6.0 14.58% 36.9 7.1 19.13% 41.1 0.8 1.98% 30.2 1.3 4.26%

11 C15:0 Pentadecanoic 21.7 0.3 1.25% 28.5 0.3 0.94% 20.7 0.2 1.02% 16.3 0.5 3.06%

7 iso-C16:0 10.2 1.4 14.10% 7.72 1.43 18.55% 10.3 0.8 8.22% 7.52 0.67 8.93%

3 C16:1n7t Palmitelaidic Acid 12.7 1.7 13.50% 29.3 3.6 12.22% 21.7 1.0 4.55% 23.1 3.9 16.93%

4 C16:1n7t Palmitelaidic Acid 152 7 4.32% 159 6 4.01% 148 4 2.90% 158 4 2.49%

7 C16:1n-9 16.4 3.4 20.63% 21.2 5.1 24.29% 17.2 1.0 5.59% 31.2 2.1 6.59%

7 C16:1n-5 285 6 1.99% 234 27 11.61% 244 9 3.51% 382 38 9.93%

7 iso-C17:0 16.8 2.5 14.92% 15.2 0.7 4.71% 16.7 1.2 7.00% 10.6 1.5 14.53%

11 C17:0 Margaric (heptadecanoic) 26.9 0.7 2.68% 33.4 0.3 0.97% 26.5 0.6 2.13% 25.0 0.4 1.54%

3 C18:1t total 18:1t 56.5 2.4 4.33% 94.1 0.6 0.68% 84.3 1.7 1.98% 132 8 5.79%

4 C18:1t 58.1 4.1 7.05% 98.3 3.7 3.72% 85.9 2.3 2.69% 123 5 4.09%

7 9t,11c 18:2 15.0 1.2 7.76% 6.52 0.66 10.07% 6.57 0.61 9.22% 6.48 0.43 6.59%

3 C18:2n6t total 18:2t 41.1 0.1 0.35% 54.0 7.1 13.22% 46.0 1.6 3.40% 62.0 4.4 7.11%

4 C18:2n6t 31.1 0.7 2.12% 37.1 0.5 1.47% 25.9 5.1 19.72% 44.9 1.5 3.31%

11 C18:4n3 Stearidonic 8.13 0.46 5.70% 4.11 0.22 5.33% 2.83 0.07 2.44% 2.57 0.06 2.37%

7 C20:3n-9 24.2 1.7 7.21% 23.9 4.9 20.34% 21.7 6.5 30.10% 25.5 2.4 9.45%

11 C20:3n9 5Z, 8Z, 11Z-Eicosatrienoic 4.67 0.12 2.49% 7.10 0.09 1.31% 7.54 0.04 0.56% 8.66 0.29 3.32%

11 C20:3n6 homo-gamma-Linolenic 102 1 1.37% 196 7 3.35% 121 0 0.29% 146 2 1.45%

7 C22 4n-3 17.2 0.7 3.84% 13.4 3.6 26.94% 10.7 0.2 2.19% 10.8 0.3 2.49%

11 C23:0 Tricosanoic 23.6 0.2 0.91% 37.9 0.6 1.57% 22.3 0.1 0.34% 21.2 0.4 1.92%

11 C26:0 Hexacosanoic (cerotic) 0.891 0.049 5.54% 0.632 0.045 7.12% 0.863 0.089 10.33% 0.549 0.073 13.25%

fatty acid (nomenclature supplied by lab)
15

Table 7. Consensus Medians (μmol/L) (excluding data from Lab 8) for each sample

Fatty acid

median n median n median n median n

C14:0 193 10 152 10 157 10 112 10

C14:1n5 14.0 7 12.1 7 12.7 7 8.55 7

C16:0 2899 10 2841 10 2503 10 2435 10

C16:1n7 201 10 263 10 176 10 271 10

C18:0 749 10 875 10 743 10 665 10

C18:1n7 141 8 135 8 120 8 134 8

C18:1n9 1910 10 2586 10 1932 10 1852 10

C18:2n6 3395 10 4354 10 3327 10 2981 10

C18:3n3 104 10 110 10 61.6 10 54.7 10

C18:3n6 40.5 10 74.5 10 50.8 10 53.2 10

C20:0 24.9 9 26.1 9 27.6 9 18.2 9

C20:1n9 16.3 8 16.1 8 17.4 8 12.7 8

C20:2n6 17.4 7 23.1 7 19.6 7 19.1 7

C20:3n6 101 9 190 9 117 9 139 9

C20:4n6 630 10 797 10 784 10 728 10

C20:5n3 263 10 70.2 10 62.5 10 42.1 10

C22:0 42.2 10 63.2 10 44.1 10 36.9 10

C22:1n9 5.76 6 7.27 6 7.27 6 6.09 6

C22:4n6 12.4 9 21.3 9 17.0 9 24.1 9

C22:5n3 65.4 10 48.8 10 32.7 10 38.4 10

C22:5n6 8.00 8 14.0 8 13.8 8 18.2 8

C22:6n3 293 10 171 10 158 10 104 10

C24:0 38.9 10 50.5 10 37.1 10 32.5 10

C24:1n9 57.3 9 52.9 9 41.9 9 47.7 9

SRM 2378-1 SRM 2378-2 SRM 2378-3 SRM 1950
16

Table 8. Percent differences of the Laboratory Means (n=3) from Median of Interlaboratory Data (See Table 7)

Laboaratory # Fatty acid SRM 2378-1 SRM 2378-2 SRM 2378-3 SRM 1950

Lab 1 C14:0 9.59% 4.11% -2.91% 4.92%

C14:1n5 33.0% 25.7% 43.0% 0.00%

C16:0 26.3% 1.04% 23.3% 1.95%

C16:1n7 6.02% 9.53% 12.6% -16.9%

C18:0 15.2% 1.27% 0.19% -6.35%

C18:1n7 20.1% -15.5% 9.49% -3.89%

C18:1n9 4.18% -0.97% 13.0% -12.1%

C18:2n6 18.0% 0.02% 1.97% 7.28%

C18:3n3 24.0% 3.58% 0.94% 12.3%

C18:3n6 20.5% 0.37% 9.19% -7.48%

C20:0 -12.7% -11.5% 19.8% 0.00%

C20:1n9 11.1% 0.56% 3.67% -10.7%

C20:2n6

C20:3n6

C20:4n6 11.6% -4.94% 4.94% 26.0%

C20:5n3 20.5% 5.42% 13.3% -1.34%

C22:0 6.24% 0.45% 0.71% -0.56%

C22:1n9 31.4% 3.75% 17.4% -11.5%

C22:4n6

C22:5n3 -3.32% 12.8% -1.21% 0.17%

C22:5n6

C22:6n3 15.0% 5.53% 0.62% 13.5%

C24:0 1.94% -11.5% -6.41% 34.0%

C24:1n9 3.47% -6.97% -9.58% 0.00%

Lab 2 C14:0 1.73% 3.52% 0.44% 2.78%

C14:1n5 55.0% 59.3% 52.2% 91.0%

C16:0 -49.2% -46.4% -47.8% -47.7%

C16:1n7 3.02% 0.41% -1.49% -7.00%

C18:0 -7.38% -10.3% -10.8% -8.04%

C18:1n7 66.6% 95.8% 76.4% 121%

C18:1n9 -41.7% -49.6% -44.3% -39.9%

C18:2n6 0.25% -6.59% -0.02% 1.64%

C18:3n3 0.32% 5.73% -0.94% -3.76%

C18:3n6 3.62% 10.0% -0.97% 3.96%

C20:0 173% 201% 113% 184%

C20:1n9

C20:2n6

C20:3n6 -41.3% -52.9% -46.9% -51.1%

C20:4n6 56.7% 67.0% 43.8% 46.6%

C20:5n3 40.6% 64.3% 39.1% 44.2%

C22:0 0.15% -6.31% -4.36% 0.56%

C22:1n9 27.2% -3.75% -17.4% 31.4%

C22:4n6 -27.4% -17.2% -27.6% -28.0%

C22:5n3 -20.5% -7.04% -12.3% -14.9%

C22:5n6 -25.0% -30.9% -37.3% -35.8%

C22:6n3 21.4% 7.33% -0.62% -9.49%

C24:0 19.7% 19.1% 17.0% 16.9%

C24:1n9 10.6% 27.2% 15.3% 8.28%
17

Table 8 (cont). Percent differences of the Laboratory Means (n=3) from Median of Interlaboratory Data (See Table 7)

Lab 3 C14:0 -2.50% 0.25% 3.86% -0.39%

C14:1n5

C16:0 -8.62% -1.04% -4.07% -9.27%

C16:1n7 -7.04% -0.41% 1.92% -0.16%

C18:0 -1.66% -1.27% -0.19% -2.03%

C18:1n7 -16.0% -19.5% -23.0% -2.12%

C18:1n9 -7.01% 0.97% -4.75% -7.30%

C18:2n6 -8.91% 0.56% -1.37% -5.87%

C18:3n3 -14.4% -12.2% -16.2% -18.3%

C18:3n6 -4.53% -4.31% -8.39% -12.6%

C20:0 9.34% 10.2% 10.7% 16.2%

C20:1n9 10.8% 28.1% 19.1% 22.3%

C20:2n6 13.7% 5.84% 4.63% 0.00%

C20:3n6 0.00% 5.73% 7.58% 1.35%

C20:4n6 -16.1% -6.32% -11.8% -13.8%

C20:5n3 -7.37% -8.29% -4.31% -11.1%

C22:0 -62.2% -56.0% -53.2% -55.1%

C22:1n9

C22:4n6 -7.09% -1.79% 15.7% 6.98%

C22:5n3 -0.53% 1.02% 12.1% 0.89%

C22:5n6 -8.47% -14.1% -7.80% -9.23%

C22:6n3 -1.97% -4.60% 9.76% 4.14%

C24:0 -68.0% -63.6% -55.7% -66.4%

C24:1n9 -68.2% -58.7% -62.2% -66.5%

Lab 4 C14:0 -19.6% -17.5% -17.8% -11.6%

C14:1n5

C16:0 -2.67% -2.78% -0.33% 3.98%

C16:1n7 -2.85% -7.95% -1.00% 0.16%

C18:0 1.66% -3.07% -1.03% 6.24%

C18:1n7

C18:1n9 -4.18% -4.82% -8.47% 0.85%

C18:2n6 -19.4% -17.0% -19.2% -12.6%

C18:3n3 -23.8% -21.3% -33.8% -21.1%

C18:3n6 -6.90% -12.9% -17.5% -10.7%

C20:0 -36.7% -41.8% -54.8% -37.0%

C20:1n9 -27.5% -23.9% -17.5% -18.5%

C20:2n6 13.0% -24.7% -15.6% -1.56%

C20:3n6 -13.2% -11.8% -12.9% -10.6%

C20:4n6 -21.7% -16.2% -23.9% -14.1%

C20:5n3 -10.3% 8.54% -1.81% 25.9%

C22:0 -49.2% -46.4% -36.5% -17.7%

C22:1n9

C22:4n6 -16.8% -16.4% -23.2% -13.9%

C22:5n3 -20.5% -20.0% -17.5% -13.1%

C22:5n6 0.00% -3.84% -16.9% 1.03%

C22:6n3 -17.8% -15.3% -21.6% -13.9%

C24:0 -75.8% -58.0% -56.1% -60.0%

C24:1n9 -62.1% -61.5% -68.8% -59.6%
18

Table 8 (cont). Percent differences of the Laboratory Means (n=3) from Median of Interlaboratory Data (See Table 7)

Lab 6 C14:0 -1.73% -6.67% -4.86% -7.67%

C14:1n5

C16:0 2.67% 4.32% -1.19% -3.78%

C16:1n7 4.51% 1.17% 2.86% 0.38%

C18:0 7.62% 3.44% 5.09% 2.03%

C18:1n7 1.68% 8.15% -1.68% -0.30%

C18:1n9 5.59% 3.64% -0.29% -0.85%

C18:2n6 -0.25% -0.01% 0.48% -2.83%

C18:3n3 -0.32% -1.84% -6.20% -2.67%

C18:3n6 0.58% -0.37% -1.10% -1.36%

C20:0

C20:1n9

C20:2n6

C20:3n6 3.18% -0.14% 0.00% 0.00%

C20:4n6 -0.69% 3.28% 2.00% -3.32%

C20:5n3 -2.27% -2.44% 0.55% -0.28%

C22:0 5.44% 10.2% 6.60% 1.65%

C22:1n9

C22:4n6 0.00% 0.00% 0.00% 0.00%

C22:5n3 1.20% -1.02% 1.21% -0.19%

C22:5n6

C22:6n3 -3.47% -8.64% -0.62% -8.11%

C24:0 8.52% 15.4% 6.41% -1.36%

C24:1n9 9.05% 16.4% 4.09% 1.01%

Lab 7 C14:0 48.5% 0.10% 42.5% 44.8%

C14:1n5 104% 23.8% 93.2% 92.6%

C16:0 34.0% -2.08% 27.3% 25.6%

C16:1n7 -72.6% -75.7% -66.8% -77.3%

C18:0 54.1% 13.9% 43.7% 40.0%

C18:1n7 6.73% -12.5% 1.68% 41.4%

C18:1n9 46.0% -4.35% 34.7% 35.7%

C18:2n6 24.5% -27.0% 19.4% 26.7%

C18:3n3 41.2% -2.77% 37.4% 39.3%

C18:3n6 41.9% -26.9% 30.3% 37.1%

C20:0 12.0% -4.23% 17.3% 103%

C20:1n9 196% 131% 67.3% 117%

C20:2n6 -51.2% -74.1% -66.4% -59.2%

C20:3n6 56.7% 10.4% 50.9% 46.7%

C20:4n6 44.1% -2.04% 33.3% 40.0%

C20:5n3 39.9% -3.14% 39.2% 46.6%

C22:0 -88.1% -96.3% -92.0% -92.0%

C22:1n9

C22:4n6 73.4% 28.2% 72.8% 71.2%

C22:5n3 52.7% 9.66% 54.9% 54.9%

C22:5n6 60.5% 19.2% 52.7% 50.5%

C22:6n3 53.5% 13.8% 59.0% 62.7%

C24:0 -90.3% -94.8% -89.0% -92.4%

C24:1n9
19

Table 8 (cont). Percent differences of the Laboratory Means (n=3) from Median of Interlaboratory Data (See Table 7)

Lab 9 C14:0 1.76% -1.41% -0.02% -1.86%

C14:1n5 10.7% 0.00% -2.09% 14.8%

C16:0 4.12% 5.42% 0.33% -1.95%

C16:1n7 3.61% -1.73% -0.40% 9.12%

C18:0 12.4% 6.11% 4.49% 8.45%

C18:1n7

C18:1n9 17.8% 13.2% 7.19% 16.2%

C18:2n6 0.25% 0.01% -2.90% -1.64%

C18:3n3 5.47% 1.84% 1.71% 2.67%

C18:3n6 7.43% 2.02% 1.73% 1.36%

C20:0 10.4% 30.3% 0.00% 40.3%

C20:1n9 -1.76% -11.0% -24.2% 2.17%

C20:2n6 12.5% 7.33% 9.44% 4.65%

C20:3n6 5.93% 0.00% -0.22% -1.75%

C20:4n6 1.22% 2.04% -7.14% -4.31%

C20:5n3 2.27% -3.99% -4.46% -3.63%

C22:0 9.37% 5.53% 5.93% 6.78%

C22:1n9 229% 124% 174% 240%

C22:4n6 141% 96.2% 91.3% 61.8%

C22:5n3 1.08% -4.82% -5.32% -0.17%

C22:5n6 13.9% -4.99% 0.09% -4.61%

C22:6n3 1.97% -6.64% -0.66% -4.14%

C24:0 9.31% 7.47% 11.1% 1.36%

C24:1n9 0.00% 0.00% 0.00% -6.23%

Lab 10 C14:0 -33.6% -43.2% -43.8% -44.9%

C14:1n5 -47.8% -54.8% -55.2% -49.6%

C16:0 -21.0% -27.6% -31.3% -32.5%

C16:1n7 -25.3% -30.6% -31.7% -32.1%

C18:0 -7.04% -20.4% -21.0% -20.9%

C18:1n7 -1.68% -3.71% -10.9% 0.30%

C18:1n9 -16.1% -23.3% -28.3% -26.4%

C18:2n6 -22.5% -26.5% -29.9% -30.2%

C18:3n3 -22.5% -28.5% -33.0% -33.1%

C18:3n6 -23.9% -27.0% -33.2% -31.3%

C20:0 3.85% 0.00% -30.7% -11.4%

C20:1n9 -0.31% -24.3% -24.7% -17.2%

C20:2n6 -19.4% -16.2% -18.6% -16.6%

C20:3n6 -6.25% -17.8% -18.3% -20.0%

C20:4n6 -15.3% -18.9% -27.4% -25.8%

C20:5n3 -13.4% -10.8% -18.8% -14.9%

C22:0 58.9% 40.1% 35.5% 32.9%

C22:1n9 -43.7% -69.7% -71.2% -65.2%

C22:4n6 5.90% -5.49% -5.69% -6.80%

C22:5n3 0.53% -9.11% -10.4% -9.83%

C22:5n6 0.00% 3.84% -0.09% -1.03%

C22:6n3 -5.75% -20.8% -15.2% -21.0%

C24:0 79.9% 62.1% 52.2% 45.5%

C24:1n9 80.7% 59.4% 52.1% 51.1%
20

Table 8 (cont). Percent differences of the Laboratory Means (n=3) from Median of Interlaboratory Data (See Table 7)

Lab 11 C14:0 2.77% -0.10% 0.02% 0.39%

C14:1n5 -12.0% -23.9% -18.0% -12.8%

C16:0 7.37% 10.9% 3.55% 5.57%

C16:1n7 2.85% 0.53% 0.40% 2.22%

C18:0 -4.67% -7.65% -11.6% -8.49%

C18:1n7 -3.29% 3.71% -8.35% -2.05%

C18:1n9 8.70% 6.89% 0.29% 2.89%

C18:2n6 0.80% 2.88% 0.02% 2.85%

C18:3n3 7.69% 7.25% 1.57% 5.18%

C18:3n6 -0.58% 2.36% 0.97% 1.94%

C20:0 0.00% 12.7% -23.2% -0.23%

C20:1n9 0.31% -0.56% -3.67% -2.17%

C20:2n6 0.00% 0.00% 0.00% 1.80%

C20:3n6 0.87% 3.38% 3.60% 5.04%

C20:4n6 0.69% 7.47% -2.00% 3.32%

C20:5n3 3.43% 2.44% -0.55% 0.28%

C22:0 39.1% 46.7% 38.4% 41.1%

C22:1n9 -26.9% -38.7% -30.8% -22.0%

C22:4n6 0.00% 8.00% 9.02% 16.9%

C22:5n3 7.83% 6.76% 11.4% 9.88%

C22:5n6 4.62% 15.0% 15.5% 18.0%

C22:6n3 9.15% 4.60% 12.2% 8.42%

C24:0 44.5% 54.4% 43.4% 39.9%

C24:1n9 52.1% 63.2% 51.5% 49.7%
21

Appendix A. CHARTS by FATTY ACID and MATERIAL

0

50

100

150

200

250

300

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 Myristic acid

0

20

40

60

80

100

120

140

160

180

200

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 Myristic acid 

Median (excluding lab 8)

193 μmol/L

Median (excluding lab 8)

152 μmol/L
22

0

50

100

150

200

250

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 Myristic acid 

0

20

40

60

80

100

120

140

160

180

200

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 Myristic acid ref value (80.1 ± 17.0) μmol/L 

Median (excluding lab 8)

157 μmol/L

Median (excluding lab 8)

112 μmol/L
23

0

5

10

15

20

25

30

35

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 Myristoleic acid

0

2

4

6

8

10

12

14

16

18

20

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 Myristoleic acid 

Median 14.0 μmol/L

Median 12.1 μmol/L
24

0

5

10

15

20

25

30

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 Myristoleic acid 

0

2

4

6

8

10

12

14

16

18

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 Myristoleic acid ref value (7.1 ± 0.1) μmol/L 

Median 12.7 μmol/L

Median 8.55 μmol/L
25

0

500

1000

1500

2000

2500

3000

3500

4000

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 Palmitic acid

0

500

1000

1500

2000

2500

3000

3500

4000

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 Palmitic acid 

Median (excluding lab 8)

2900 μmol/L

Median (excluding lab 8)

2840 μmol/L
26

0

500

1000

1500

2000

2500

3000

3500

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 Palmitic acid 

0

500

1000

1500

2000

2500

3000

3500

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 Palmitic acid cert value (2364 ± 77) μmol/L 

Median (excluding lab 8)

2500 μmol/L

Median (excluding lab 8)

2440 μmol/L
27

0

50

100

150

200

250

300

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 Palmitoleic acid

0

50

100

150

200

250

300

350

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 Palmitoleic acid 

Median (excluding lab 8) 

201 μmol/L

Median (excluding lab 8)

263 μmol/L
28

0

50

100

150

200

250

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 Palmitoleic acid 

0

50

100

150

200

250

300

350

400

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 Palmitoleic acid cert value (215 ± 26) μmol/L 

Median (excluding lab 8)

176 μmol/L

Median (excluding lab 8)

271 μmol/L
29

0

200

400

600

800

1000

1200

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 Stearic acid

0

200

400

600

800

1000

1200

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 Stearic acid 

Median (excluding lab 8)

749 μmol/L

Median (excluding lab 8)

875 μmol/L
30

0

200

400

600

800

1000

1200

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 Stearic acid 

0

200

400

600

800

1000

1200

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 Stearic acid cert value (644 ± 41) μmol/L 

Median (excluding lab 8)

743 μmol/L

Median (excluding lab 8)

665 μmol/L
31

0

50

100

150

200

250

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 cis-Vaccenic acid

0

50

100

150

200

250

300

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 cis-Vaccenic acid 

Median (excluding lab 8)

141 μmol/L

Median (excluding lab 8)

135 μmol/L
32

0

50

100

150

200

250

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 cis-Vaccenic acid 

0

50

100

150

200

250

300

350

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 cis-Vaccenic acid ref value (136 ± 3) μmol/L 

Median (excluding lab 8)

120 μmol/L

Median (excluding lab 8)

134 μmol/L
33

0

500

1000

1500

2000

2500

3000

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 Oleic acid

0

500

1000

1500

2000

2500

3000

3500

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 Oleic acid 

Median (excluding lab 8)

1910 μmol/L

Median (excluding lab 8)

2590 μmol/L
34

0

500

1000

1500

2000

2500

3000

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 Oleic acid 

0

500

1000

1500

2000

2500

3000

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 Oleic acid cert value (1614 ± 154) μmol/L 

Median (excluding lab 8)

1930 μmol/L

Median (excluding lab 8)

1850 μmol/L
35

0

500

1000

1500

2000

2500

3000

3500

4000

4500

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 Linoleic acid

0

1000

2000

3000

4000

5000

6000

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 Linoleic acid 

Median (excluding lab 8)

3400 μmol/L

Median (excluding lab 8)

4350 μmol/L
36

0

500

1000

1500

2000

2500

3000

3500

4000

4500

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 Linoleic acid 

0

500

1000

1500

2000

2500

3000

3500

4000

4500

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 Linoleic acid cert value (2838 ± 143) μmol/L 

Median (excluding lab 8)

3330 μmol/L

Median (excluding lab 8)

2980 μmol/L
37

0

20

40

60

80

100

120

140

160

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 alpha-Linolenic acid

0

20

40

60

80

100

120

140

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 alpha-Linolenic acid 

Median 104 μmol/L

Median 110 μmol/L
38

0

10

20

30

40

50

60

70

80

90

100

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 alpha-Linolenic acid 

0

10

20

30

40

50

60

70

80

90

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 alpha-Linolenic acid cert value (54.6 ± 3.6) μmol/L 

Median 61.6 μmol/L

Median 54.7 μmol/L
39

0

10

20

30

40

50

60

70

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 gamma-Linolenic acid

0

10

20

30

40

50

60

70

80

90

100

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 gamma-Linolenic acid 

Median (excluding lab 8)

40.5 μmol/L

Median (excluding lab 8)

74.5 μmol/L
40

0

10

20

30

40

50

60

70

80

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 gamma-Linolenic acid 

0

10

20

30

40

50

60

70

80

90

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 gamma-Linolenic acid ref value (39.9 ± 8.5) μmol/L 

Median (excluding lab 8)

50.8 μmol/L

Median (excluding lab 8)

53.2 μmol/L
41

0

10

20

30

40

50

60

70

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 Arachidic acid

0

10

20

30

40

50

60

70

80

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 Arachidic acid 

Median 24.9 μmol/L

Median 26.1 μmol/L
42

0

10

20

30

40

50

60

70

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 Arachidic acid 

0

10

20

30

40

50

60

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 Arachidic acid ref value (18.0 ± 0.5) μmol/L 

Median 27.6 μmol/L

Median 18.2 μmol/L
43

0

10

20

30

40

50

60

70

80

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 11-Eicosenoic acid

0

10

20

30

40

50

60

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 11-Eicosenoic acid 

Median 16.3 μmol/L

Median 16.1 μmol/L
44

0

5

10

15

20

25

30

35

40

45

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 11-Eicosenoic acid 

0

5

10

15

20

25

30

35

40

45

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 11-Eicosenoic acid ref value (11.5 ± 0.5) μmol/L 

Median 17.4 μmol/L

Median 12.7 μmol/L
45

0

5

10

15

20

25

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 11,14-Eicosadienoic acid

0

5

10

15

20

25

30

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 11,14-Eicosadienoic acid 

Median 17.4 μmol/L

Median 23.1 μmol/L
46

0

5

10

15

20

25

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 11,14-Eicosadienoic acid 

0

5

10

15

20

25

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 11,14-Eicosadienoic Acid 

ref value (18.8 ± 0.6) μmol/L 

Median 19.6 μmol/L

Median 19.1 μmol/L
47

0

20

40

60

80

100

120

140

160

180

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 homo-gamma-Linolenic acid

0

50

100

150

200

250

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 homo-gamma-Linolenic acid 

Median (excluding lab 8)

190 μmol/L

Median (excluding lab 8)

101 μmol/L
48

0

20

40

60

80

100

120

140

160

180

200

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 homo-gamma-Linolenic acid 

0

50

100

150

200

250

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 homo-gamma-Linolenic Acid 

ref value (139 ± 4) μmol/L 

Median (excluding lab 8)

117 μmol/L

Median (excluding lab 8)

139 μmol/L
49

0

200

400

600

800

1000

1200

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 Arachidonic acid 

0

200

400

600

800

1000

1200

1400

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 Arachidonic acid 

Median (excluding lab 8)

630 μmol/L

Median (excluding lab 8)

797 μmol/L
50

0

200

400

600

800

1000

1200

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 Arachidonic acid 

0

200

400

600

800

1000

1200

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 Arachidonic acid 

ref value (984 ± 180) μmol/L 

Median (excluding lab 8)

784 μmol/L

Median (excluding lab 8)

728 μmol/L
51

0

50

100

150

200

250

300

350

400

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 EPA

0

20

40

60

80

100

120

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 EPA

Median (excluding lab 8)

263 μmol/L

Median 70.2 μmol/L
52

0

10

20

30

40

50

60

70

80

90

100

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 EPA

0

10

20

30

40

50

60

70

80

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 EPA ref value (38.6 ± 0.5) μmol/L 

Median 62.5 μmol/L

Median 42.1 μmol/L
53

0

10

20

30

40

50

60

70

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 Docosanoic acid

0

10

20

30

40

50

60

70

80

90

100

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 Docosanoic acid

Median 42.2 μmol/L

Median 63.2 μmol/L
54

0

10

20

30

40

50

60

70

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 Docosanoic acid

0

10

20

30

40

50

60

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 Docosanoic acid cert value (47.8 ± 6.0) μmol/L 

Median 44.1 μmol/L

Median 36.9 μmol/L
55

0

5

10

15

20

25

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 Docosenoic acid 

0

2

4

6

8

10

12

14

16

18

20

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 Docosenoic acid

Median 5.76 μmol/L

Median 7.27 μmol/L
56

0

5

10

15

20

25

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 Docosenoic acid

0

5

10

15

20

25

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 Docosenoic acid ref value (3.4 ± 1.3) μmol/L 

Median 7.27 μmol/L

Median 6.09 μmol/L
57

0

5

10

15

20

25

30

35

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 Docosatetraenoic acid

0

5

10

15

20

25

30

35

40

45

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 Docosatetraenoic acid

Median 12.4 μmol/L

Median 21.3 μmol/L
58

0

5

10

15

20

25

30

35

40

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 Docosatetraenoic acid

0

10

20

30

40

50

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 Docosatetraenoic acid 

ref value (25.5 ± 0.6) μmol/L 

Median 17.0 μmol/L

Median (excluding lab 8)

24.1 μmol/L
59

0

20

40

60

80

100

120

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 Docosapentaenoic (n3) acid 

0

10

20

30

40

50

60

70

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 Docosapentaenoic (n3) acid

Median 65.4 μmol/L

Median 48.8 μmol/L
60

0

10

20

30

40

50

60

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 Docosapentaenoic (n3) acid

0

10

20

30

40

50

60

70

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 Docosapentaenoic (n3) acid 

ref value (38.5 ± 0.7) μmol/L 

Median 32.7 μmol/L

Median (excluding lab 8)

38.4 μmol/L
61

0

2

4

6

8

10

12

14

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 Docosapentaenoic (n6) acid 

0

5

10

15

20

25

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 Docosapentaenoic (n6) acid

Median 8.00 μmol/L

Median 14.0 μmol/L
62

0

5

10

15

20

25

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 Docosapentaenoic (n6) acid

0

5

10

15

20

25

30

35

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 Docosapentaenoic (n6) acid 

ref value (19.5 ± 0.4) μmol/L 

Median 13.8 μmol/L

Median 18.2 μmol/L
63

0

50

100

150

200

250

300

350

400

450

500

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 DHA

0

50

100

150

200

250

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 DHA

Median (excluding lab 8)

293 μmol/L

Median (excluding lab 8)

171 μmol/L
64

0

50

100

150

200

250

300

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 DHA

0

20

40

60

80

100

120

140

160

180

200

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 DHA ref value (118 ± 21) μmol/L 

Median (excluding lab 8)

158 μmol/L

Median (excluding lab 8)

104 μmol/L
65

0

10

20

30

40

50

60

70

80

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 Lignoceric acid

0

10

20

30

40

50

60

70

80

90

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 Lignoceric acid 

Median (excluding lab 8)

38.9 μmol/L

Median (excluding lab 8)

50.5 μmol/L
66

0

10

20

30

40

50

60

70

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 Lignoceric acid 

0

5

10

15

20

25

30

35

40

45

50

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 Lignoceric acid ref value (46.6 ± 2.6) μmol/L 

Median (excluding lab 8)

37.1 μmol/L

Median 32.5 μmol/L
67

0

20

40

60

80

100

120

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-1 Nervonic acid

0

10

20

30

40

50

60

70

80

90

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-2 Nervonic acid 

Median 57.3 μmol/L

Median 52.9 μmol/L
68

0

10

20

30

40

50

60

70

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 2378-3 Nervonic acid 

0

10

20

30

40

50

60

70

80

0 1 2 3 4 5 6 7 8 9 10 11

μmol/L

Lab #

SRM 1950 Nervonic acid ref value (71.3 ± 3.2) μmol/L 

Median 41.9 μmol/L

Median 47.7 μmol/L
69

Table A-1 Procedures used

Lab # 1 2 3 4 5

Reporting date 10/19/2012 6/25/2012 7/23/2012 7/29/2012 7/31/2012

Volume of sample extracted (mL): 0.0125 mL 0.0125 mL

Mass of samples extracted (g) (average of 3):

SRM 2378-1 0.93 g 0.096 g 0.012 g 0.100 g

SRM 2378-2 0.90 g 0.098 g 0.013 g 0.100 g

SRM 2378-3 0.90 g 0.097 g 0.014 g 0.100 g

SRM 1950 0.90 g 0.096 g 0.013 g 0.100 g

Analytical method reference, if available:

Lagerstadt SA, Hinrichs DR, Batt SM, Magera MJ, Rinaldo 

P, McConnell JP. (2001) Quantitative determination of 

plasma C8-C26 total fatty acids for the biochemical 

diagnosis of nutritional and metabolic disorders. Mol 

Genet Metab ,.73:38-45

Analytical method used:

Hydrolysis method 0.4 M potassium hydroxide in methanol Acid (0.6N HCl) and base (1N NaOH) hydrolysis BF3 Methanol BF3 Methanol Folch

Extraction method liquid-liquid Manual extraction

Extraction solvent hexane Hexane 3mL of 2:1 chloroform:methanol

Extraction time

vortex for 30 min and centrifuge for 5 min - repeat 3 x 

with 2 mL of hexane each time 2 minutes 1 minute during vortex in 2:1 chloroform:methanol

Extraction - other details combine hexane phases none

Surrogate standard added to 2:1 chloroform:methanol 

solution

Sample extract cleanup method hexane extraction

add 500uL sodium phosphate buffer, invert twice, 

centrifuge @ 1734g for 5 min; extract bottom chloroform 

layer, dry down under nitrogen, derivitize with 1mL 14% 

BF3 in methanol with 0.3mL hexane; Add 1mL hexane, 

1mL water, vortex for 1 min, centrifuge @ 1734g for 3 

min; Extract top hexane layer, dry down under nitrogen, 

reconstitute in 65uL heptane 

Derivitization reagent

5% (v/v) sulfuric acid in methanol - heat 80 deg C for 30 

min - extract 3 x with 2 mL hexane each time Pentafluorobenzyl Bromide (PFBBr) BF3 Methanol BF3 Methanol

Analytical instrument GC-FID

Agilent 6890 series gas chromatograph with Agilent 5973 

CI source MS GC-FID GC-FID Varian 3900

Column phase SP2560 Crosslinked 5% Diphenyl and 95% Dimethylpolysiloxane SP-2560 SP-2560 DB-FFAP

Column length, m 100 m 25 100 100 15

Column i.d., mm 0.25 0.200 0.25 0.25 0.1

Column film thickness, μm 0.25 0.33 0.2 0.2 0.1

Injection method (split, splitless, etc) split 30:1 split (split ratio 105:1) and splitless Split Split split

Method of quantitation:

ES = external standards (Y/N) Y Yes Y

Number of ES used 10 3 3

 IS = internal standards (Y/N) y Y Yes Y y

Number of IS used 2 13 2 1 1

IS added PRIOR to extraction of sample (Y/N) y Y Yes Y y

Additional data/information 
70

Table A-1 Continued

Lab # 6 7 8 9 10

Reporting date 7/31/2012 7/31/2012 7/31/2012 11/2/2012 11/8/2012

Volume of sample extracted (mL): 0.2 mL 0.2 mL 0.3 mL 0.2 mL 0.100 mL

Mass of samples extracted (g) (average of 3):

SRM 2378-1 0.208 g 0.199 g 0.300 g 0.200 g

SRM 2378-2 0.208 g 0.199 g 0.301 g 0.200 g

SRM 2378-3 0.208 g 0.198 g 0.306 g 0.200 g

SRM 1950 0.207 g 0.198 g 0.299 g 0.200 g

Analytical method reference, if available:

Combined and modified three published methods: 

Masood et al. 2004 for sample prep, David et al. 2005 for 

GC, Dodds et al. 2005 for MS (see below)

1) Lepage G, Roy CC. J Lipid Res 1986; 27:114-120. 

2) Lin YH, et al. Lipids 2012; 47:527-39.

Analytical method used:

Hydrolysis method One-step hydrolysis/derivatization with acetyl chloride None n/a

Extraction method

Manual sequential extraction (2mL extraction solvent 

each time, three times) Bligh and Dyer Solvent extraction Modified Folch n/a

Extraction solvent Hexane Methanol/Chlorform/Water 2/1/0.8 Aqueous methanol Chloroform/Methanol (2:1) n/a

Extraction time

Minimum 5 minutes between the addition of hexane 

and transfer of hexane extract (for each time) 30 mins on a vortexer 1 min, two times_ 20 minutes n/a

Extraction - other details

Vortex-mix twice, plasma and serum samples were 

centrifuged for 5 minutes at 1000 rpm Constant vortexing

One-step, direct transmethylation analysis 

without previous lipids extraction

Sample extract cleanup method centrifuge, 5 min at 10000 rpm 1 N NaCl wash n/a

Derivitization reagent

5mL acetyl chloride added to 86.2mL methanol with 50 

mg/L butylated hydroxytoluene, incubation at 90 C for 45 

min 14% Boron Triflouride in Methanol Methyl chloroformate (MCF) Boron trichloride methanol : hexane : acetyl chloride (4:1:0.5, v/v/v) 

Analytical instrument Agilent GC/MSD (6890N/5973N) with EI ionization

HP 5890 GCFID quantification. Peak ID with Varian 

Saturn 2000 GC-(CACI)MSMS, column SGE BPX70 Thermo TraceGC Ultra ISQ Varian 3400 Agilent 7890A fast gas chromatograph

Column phase

DB-23 which is (50%-Cyanopropyl)-methylpolysiloxane 

70% Cyanopropyl polysilphenylene-siloxane

TG-1701MS, 14% Cyanopropylphenyl 

Methylpolysiloxane DB-23 high efficient DB-FFAP

Column length, m 60 25 15 60.00 15.0

Column i.d., mm 0.25 0.22 0.25 0.32 0.10

Column film thickness, μm 0.15 0.25 0.25 150.00 0.10

Injection method (split, splitless, etc)

Injected same samples twice, at 50:1 and 500:1 split 

ratios, respectively Splitless Splitless Septum Programable Injector (SPI)

split 50 : 1 (hydrogen as carrier gas, nitrogen as 

makeup gas)

Method of quantitation:

ES = external standards (Y/N) Y N N N Y

Number of ES used 18 0 23

 IS = internal standards (Y/N) Y Y Y Y Y

Number of IS used 4 1 IS for each compound 1 one

IS added PRIOR to extraction of sample (Y/N) Y Y Y Y Y

Additional data/information The references mentioned above are:

Signal response of each FAME on the GC-FID was 

corrected using an equal wt standard from Nucheck Prep 

cat# 68a 

(Internal standard / fatty acids) ratio 

calibration used

Masood, A. et al. (2005) J. Lipid. Res. 46:2299-2305.

David, F. et al. (2009) Agilent Technologies Application 

Notes. 5990-4822EN.

Dodds, E.D. et al. (2005) Lipids. 40:419-428.

C16:0, C18:0, C18:1n9, C18:2n6 and C20:4n6 were 

quantified by injecting the samples at 500:1 split ratio, 

the other analytes at 50:1 split ratio
71

Table A-1 Continued

Lab # 11

Reporting date 1/10/2013

Volume of sample extracted (mL): 0.10 mL

Mass of samples extracted (g) (average of 3):

SRM 2378-1 0.0967 g

SRM 2378-2 0.0983 g

SRM 2378-3 0.0987 g

SRM 1950 0.0973 g

Analytical method reference, if available:

Lagertedt S, Hinrichs D, Batt S, Magera M, Rinaldo P, McConnell 

J. Quantitative Determination of Plasma C8-C26 Total Fatty 

Acids for the Biochemical Diagnosis of Nutritional and Metabolic 

Disorders. Mol Genet & Metab 2001; 73: 38-45.

Analytical method used:

Hydrolysis method Acid (0.6N HCl) and base (1N NaOH) hydrolysis

Extraction method Liquid:liquid

Extraction solvent hexane

Extraction time 20 to 30 minutes per extraction set

Extraction - other details triple extraction via automated liquid handler

Sample extract cleanup method Speedvac

Derivitization reagent PFBBr-pentafluorobenzyl bromide

Analytical instrument GC/MS

Column phase cyanopropyl-methylpolysiloxane phase (50-50)

Column length, m 60

Column i.d., mm 0.25

Column film thickness, μm 0.25

Injection method (split, splitless, etc) split

Method of quantitation:

ES = external standards (Y/N) N

Number of ES used

 IS = internal standards (Y/N) Y

Number of IS used 18

IS added PRIOR to extraction of sample (Y/N) Y

Additional data/information 
72

Table A-2 Calibration information 

Lab Fatty Acid 

Code Fatty Acid Name

Lowest 

std 

(μmol/L)

Highest 

std 

(μmol/L)

LOD 

(μmol/L)

LOQ 

(μmol/L)

Curve 

Type Weighting IS used for quantitation

Lab 1 C14:0 Myristic acid 5 500 linear palmitic acid -d 31

C14:1n5 Myristoleic acid 1.5 140 linear palmitic acid -d 31

C16:0 Palmitic acid 5.5 550 linear palmitic acid -d 31

C16:1n7 Palmitoleic acid 3.5 360 linear palmitic acid -d 31

C18:0 Stearic acid 5.5 560 linear palmitic acid -d 31

C18:1n7 cis- Vaccenic acid 1.5 175 linear palmitic acid -d 31

C18:1n9 Oleic acid 5.5 525 linear palmitic acid -d 31

C18:2n6 Linoleic acid 5.5 550 linear palmitic acid -d 31

C18:3n3 alpha- Linolenic acid 2.5 320 linear palmitic acid -d 31

C18:3n6 gamma- Linolenic acid 1 140 linear palmitic acid -d 31

C20:0 Arachidic acid 2.5 275 linear palmitic acid -d 31

C20:1n9 11-Eicosenoic acid 1 150 linear palmitic acid -d 31

C20:2n6 11,14-Eicosadienoic acid 

C20:3n6 homo-gamma- Linolenic acid 

C20:4n6 Arachidonic acid 1 150 linear heneicosanoic acid

C20:5n3 Eicosapentaenoic acid 1 150 linear heneicosanoic acid

C22:0 Docosanoic acid 3 320 linear heneicosanoic acid

C22:1n9 Docosenoic acid 1.5 175 linear heneicosanoic acid

C22:4n6 Docosatetraenoic acid 

C22:5n3 Docosapentaenoic acid 1 150 linear heneicosanoic acid

C22:5n6 Docosapentaenoic acid 

C22:6n3 Docosahexaenoic acid 1 150 linear heneicosanoic acid

C24:0 Lignoceric acid 1 150 linear heneicosanoic acid

C24:1n9 Nervonic acid 1 150 linear heneicosanoic acid

Lab 2 C8:0 Octanoic 12.5 100 0.616 ND linear equal 13C4-octanoic

C10:1 Decenoic 12.5 100 0.193 ND linear equal d3-decanoic

C10:0 Decanoic 12.5 100 0.193 ND linear equal d3-decanoic

C12:1 Lauroleic 12.5 100 0.642 ND linear equal d3-lauric

C12:0 Lauric 12.5 100 0.642 ND linear equal d3-lauric

C14:2 Tetradecenoic 12.5 100 0.048 ND linear equal d3-pentadecanoic

C14:1 Myristoleic 12.5 100 0.048 ND linear equal d3-pentadecanoic

C16:2 Hexadecenoic 12.5 100 0.048 ND linear equal d3-pentadecanoic

C20:0 Arachidic 125 1000 0.282 ND linear equal d3-arachidic

C20:3ω9 Mead 6.25 50 0.023 ND linear equal d4-docosanoic

C20:3ω6 homo -γ-Linoleic 6.25 50 0.023 ND linear equal d4-docosanoic

C22:5ω3 Docosapentaenoic (ω3) 6.25 50 0.021 ND linear equal d4-docosanoic

C22:5ω6 Docosapentaenoic (ω6) 6.25 50 0.021 ND linear equal d4-docosanoic
73

Table A-2 Continued

Lab Fatty Acid 

Code Fatty Acid Name

Lowest 

std 

(μmol/L)

Highest 

std 

(μmol/L)

LOD 

(μmol/L)

LOQ 

(μmol/L)

Curve 

Type Weighting IS used for quantitation

Lab 2 C22:1ω9 Erucic 6.25 50 0.022 ND linear equal d4-docosanoic

C22:0 Docosanoic 6.25 50 0.055 ND linear equal d4-docosanoic

C24:1ω9 Nervonic 6.25 50 0.015 ND linear equal d4-tetracosanoic

C24:0 Tetracosanoic 6.25 50 0.054 ND linear equal d4-tetracosanoic

C26:1 Hexacosenoic 1.25 10 0.018 ND linear equal d4-hexacosanoic

C26:0 Hexacosanoic 1.25 10 0.018 ND linear equal d4-hexacosanoic

PhA Phytanic 2.5 20 0.008 ND linear equal d3-phytanic

PrA Pristanic 0.25 2.0 0.003 ND linear equal d3-pristanic

C14:0 Myristic 125 1000 0.783 ND linear equal d3-myristic

C16:1ω9 7-Hexadecenoic 125 1000 0.057 ND linear equal d3-myristic

C16:1ω7 Palmitoleic 125 1000 0.057 ND linear equal d3-myristic

C16:0 Palmitic 250 2000 5.68 ND linear equal d3-palmitic

C18:2ω6 Linoleic 250 2000 1.13 ND linear equal d3-stearic

C18:1ω9 Oleic 250 2000 1.419 ND linear equal d3-stearic

C18:1ω7 Vaccenic 250 2000 1.419 ND linear equal d3-stearic

C18:0 Stearic 250 2000 8.312 ND linear equal d3-stearic

C18:3ω6 γ-Linolenic 125 1000 0.042 ND linear equal d3-arachidic

C18:3ω3 Linolenic 125 1000 0.042 ND linear equal d3-arachidic

C20:5ω3 Eicosapentaenoic 125 1000 0.241 ND linear equal d3-arachidic

C20:4ω6 Arachidonic 125 1000 0.13 ND linear equal d3-arachidic

C22:6ω3 Docosahexaenoic 6.25 50 0.029 ND linear equal d4-docosanoic

C22:4ω6 Docosatetraenoic 6.25 50 0.13 ND linear equal d4-docosanoic

Lab 3 C14:0 Myristic acid 0.1 2.5 Linear None

C16:0 Palmitic acid 1.6 40 Linear None

C16:1n7t Palmitelaidic Acid 0.05 1.25 Linear None

C16:1n7 Palmitoleic acid 0.1 2.5 Linear None

C17:0 1 25 Linear None

C18:0 Stearic acid 1.2 30 Linear None

C18:1t 0.1 2.5 Linear None

C18:1n9 Oleic acid 1.1 27.5 Linear None

C18:2n6t Linoelaidic Acid 0.05 1.25 Linear None

C18:2n6 Linoleic acid 1 25 Linear None

C20:0 Arachidic acid 0.1 2.5 Linear None

C18:3n6 gamma-Linolenic acid 0.05 1.25 Linear None

C20:1n9 11-Eicosenoic acid 0.15 3.75 Linear None

C18:3n3 alpha-Linolenic acid 0.05 1.25 Linear None

C20:2n6 11,14-Eicosadienoic acid 0.15 3.75 Linear None

C22:0 Docosanoic acid 0.1 2.5 Linear None
74

Table A-2 Continued

Lab Fatty Acid 

Code Fatty Acid Name

Lowest 

std 

(μmol/L)

Highest 

std 

(μmol/L)

LOD 

(μmol/L)

LOQ 

(μmol/L)

Curve 

Type Weighting IS used for quantitation

Lab 3 C20:3n6 homo-gamma-Linolenic acid 0.3 7.5 Linear None

C20:4n6 Arachidonic acid 1.2 30 Linear None

C24:0 Lignoceric acid 0.1 2.5 Linear None

C20:5n3 Eicosapentaenoic acid 0.3 7.5 Linear None

C24:1n9 Nervonic acid 0.1 2.5 Linear None

C22:3n3 25 25 Linear None

C22:4n6 Docosatetraenoic acid 0.2 5 Linear None

C22:5n6 Docosapentaenoic acid 0.1 2.5 Linear None

C22:5n3 Docosapentaenoic acid 0.3 7.5 Linear None

C22:6n3 Docosahexaenoic acid 0.4 10 Linear None

C14:1n5 Myristoleic acid 

C18:1n7 cis-Vaccenic acid 1.1 27.5 Linear None

C22:1n9 Docosenoic acid 

Lab 4 C14:0 Myristic acid 0.1 2.5 Linear None

C16:0 Palmitic acid 1.6 40 Linear None

C16:1n7t Palmitelaidic Acid 0.05 1.25 Linear None

C16:1n7 Palmitoleic acid 0.1 2.5 Linear None

C18:0 Stearic acid 1.2 30 Linear None

C18:1t 0.1 2.5 Linear None

C18:1n9 Oleic acid 1.1 27.5 Linear None

C18:2n6t Linoelaidic Acid 0.05 1.25 Linear None

C18:2n6 Linoleic acid 1 25 Linear None

C20:0 Arachidic acid 0.1 2.5 Linear None

C18:3n6 gamma-Linolenic acid 0.05 1.25 Linear None

C20:1n9 11-Eicosenoic acid 0.15 3.75 Linear None

C18:3n3 alpha-Linolenic acid 0.05 1.25 Linear None

C20:2n6 11,14-Eicosadienoic acid 0.15 3.75 Linear None

C22:0 Docosanoic acid 0.1 2.5 Linear None

C20:3n6 homo-gamma-Linolenic acid 0.3 7.5 Linear None

C20:4n6 Arachidonic acid 1.2 30 Linear None

C24:0 Lignoceric acid 0.1 2.5 Linear None

C20:5n3 Eicosapentaenoic acid 0.3 7.5 Linear None

C24:1n9 Nervonic acid 0.1 2.5 Linear None

C22:3n3 25 25 Linear None

C22:4n6 Docosatetraenoic acid 0.2 5 Linear None

C22:5n6 Docosapentaenoic acid 0.1 2.5 Linear None

C22:5n3 Docosapentaenoic acid 0.3 7.5 Linear None

C22:6n3 Docosahexaenoic acid 0.4 10 Linear None
75

Table A-2 Continued

Lab Fatty Acid 

Code Fatty Acid Name

Lowest 

std 

(μmol/L)

Highest 

std 

(μmol/L)

LOD 

(μmol/L)

LOQ 

(μmol/L)

Curve 

Type Weighting IS used for quantitation

Lab 5 No information

Lab 6 C14:0 Myristic 12.60 440.63 1.09 quadratic 1/x C13:1n1 FAME

C16:0 Palmitic 233.66 7379.21 35.81 quadratic 1/x C17:1n7 trans FAME

C16:1n7 Palmitoleic 23.47 740.53 4.03 quadratic 1/x C13:1n1 FAME

C18:0 Stearic 100.11 3503.59 6.97 quadratic 1/x C17:1n7 trans FAME

C18:1n9 Oleic 214.02 6758.48 36.77 quadratic 1/x C17:1n7 trans FAME

C18:1n7 Vaccenic 21.31 673.38 2.49 quadratic 1/x C21:0 FAME

C18:2n6 Linoleic 214.76 6782.35 26.76 quadratic 1/x C17:1n7 trans FAME

C18:3n6 gamma-Linolenic 10.34 361.85 0.83 quadratic 1/x C21:0 FAME

C18:3n3 alpha-Linolenic 10.24 357.83 1.22 quadratic 1/x C21:0 FAME

C20:3n6 Eicosatrienoic 20.03 632.10 2.90 quadratic 1/x C21:0 FAME

C20:4n6 Arachidonic 92.68 3243.48 5.07 quadratic 1/x C17:1n7 trans FAME

C22:0 Behenic 8.25 288.84 0.66 quadratic 1/x C21:0 FAME

C20:5n3 Eicosapentaenoic 9.59 335.07 1.41 quadratic 1/x C23:1n9 FAME

C22:4n6 Docosatetraenoic 8.60 300.90 0.70 quadratic 1/x C23:1n9 FAME

C24:0 Lignoceric 7.81 272.99 0.55 quadratic 1/x C23:1n9 FAME

C22:5n3 Docosapentaenoic 8.62 301.39 0.67 quadratic 1/x C23:1n9 FAME

C24:1 Nervonic 7.72 270.62 1.14 quadratic 1/x C23:1n9 FAME

C22:6n3 Docosahexaenoic 18.48 583.11 1.76 quadratic 1/x C23:1n9 FAME

Lab 7 No information

Lab 8 C14:0 Myristic 0.66 876.39 1.12 3.39 linear myristic-d3

C14:1n5 Myristoleic 0.33 442.11 0.92 2.79 linear myristoleic-d3

C16:0 Palmitic 0.88 870.78 2.63 7.97 linear palmitic-d3

C16:1n7 Palmitoleic 0.59 393.36 0.84 2.55 linear palmitoleic-d3

C18:0 Stearic 1.06 703.56 2.74 8.30 linear stearic-d3

C18:1n7 cis-Vaccenic 1.06 598.32 1.36 4.12 linear cis-vaccenic-d3

C18:1n9 Oleic 1.07 708.57 1.79 5.42 linear oleic-d3

C18:2n6 Linoleic 1.35 356.84 0.97 2.94 linear linoleic-d3

C18:3n3 alpha-Linolenic 1.16 412.79 1.02 3.09 linear alpha-linolenic-d3

C18:3n6 gamma-Linolenic 1.36 459.43 1.01 3.06 linear gamma-linolenic-d3

C20:0 Arachidic 0.97 640.41 2.54 7.70 linear arachidic-d3

C20:1n9 11-Eicosenoic 0.81 422.28 2.03 6.15 linear 11-eicosenoic-d3

C20:2n6 11,14-Eicosadienoic 0.82 424.39 1.03 3.12 linear 11,14-eicosadienoic -d3

C20:3n6 h-g-Linolenic 0.51 415.94 0.96 2.91 linear h-g-linolenic -d3

C20:4n6 Arachidonic 0.83 428.69 1.06 3.21 linear arachidonic-d3

C20:5n3 Eicosapentaenoic 0.89 487.37 1.23 3.73 linear eicosapentaenoic-d3

C22:0 Docosanoic 1.19 521.24 1.08 3.27 linear docosanoic-d3

C22:1n9 Docosenoic 1.25 654.40 1.23 3.73 linear docosenoic-d3
76

Table A-2 Continued

Lab Fatty Acid 

Code Fatty Acid Name

Lowest 

std 

(μmol/L)

Highest 

std 

(μmol/L)

LOD 

(μmol/L)

LOQ 

(μmol/L)

Curve 

Type Weighting IS used for quantitation

Lab 8 C22:4n6 Docosatetraenoic 1.24 459.37 0.5 1.52 linear docosatetraenoic-d3

C22:5n3 Docosapentaenoic 1.26 423.37 0.48 1.45 linear docosapentaenoic-d3

C22:6n3 Docosahexaenoic 0.77 504.66 0.89 2.70 linear docosahexaenoic-d3

C24:0 Lignoceric 1.02 542.93 2.78 8.42 linear lignoceric -d3

C24:1n9 Nervonic 1.03 472.96 1.25 3.79 linear nervonic -d3

Lab 9 C14:0 1.0097 tritridecanoin

C14:1 1.0012 tritridecanoin

C15:0 1.0207 tritridecanoin

C16:0 1.0214 tritridecanoin

C16:1 1.0337 tritridecanoin

C18:0 1.0370 tritridecanoin

C18:1 total 1.0382 tritridecanoin

C18:2n6 1.0432 tritridecanoin

C18:3n6 1.0492 tritridecanoin

C18:3n3 1.0511 tritridecanoin

C18:4n3 tritridecanoin

C20:0 1.0129 tritridecanoin

C20:1 total 1.0159 tritridecanoin

C20:2n6 1.0266 tritridecanoin

C20:3n6 1.0222 tritridecanoin

 C20:4n6 1.0521 tritridecanoin

C20:3n3 1.0121 tritridecanoin

C20:4n3 tritridecanoin

C20:5n3 1.0341 tritridecanoin

C22:0 0.9930 tritridecanoin

C22:1total 0.9914 tritridecanoin

C22:2n6 1.0039 tritridecanoin

C22:4n6 1.0051 tritridecanoin

C22:5n6 1.0324 tritridecanoin

C22:5n3 0.9949 tritridecanoin

C22:6n3 0.9825 tritridecanoin

C24:0 0.9862 tritridecanoin

C24:1 0.9740 tritridecanoin

Lab 10 C14:0 Myristic acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester

C14:1n5 Myristoleic acid n/a 277 n/a n/a one point n/a 22:3n-3 ethyl ester

C16:0 Palmitic acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester

C16:1n7 Palmitoleic acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester

C18:0 Stearic acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester
77

Table A-2 Continued

Lab Fatty Acid 

Code Fatty Acid Name

Lowest 

std 

(μmol/L)

Highest 

std 

(μmol/L)

LOD 

(μmol/L)

LOQ 

(μmol/L)

Curve 

Type Weighting IS used for quantitation

Lab 10 C18:1n7 cis- Vaccenic acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester

C18:1n9 Oleic acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester

C18:2n6 Linoleic acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester

C18:3n3 alpha- Linolenic acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester

C18:3n6 gamma- Linolenic acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester

C20:0 Arachidic acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester

C20:1n9 11-Eicosenoic acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester

C20:2n6 11,14-Eicosadienoic acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester

C20:3n6 homo-gamma- Linolenic acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester

C20:4n6 Arachidonic acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester

C20:5n3 Eicosapentaenoic acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester

C22:0 Docosanoic acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester

C22:1n9 Docosenoic acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester

C22:4n6 Docosatetraenoic acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester

C22:5n3 Docosapentaenoic acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester

C22:5n6 Docosapentaenoic acid n/a 277 n/a n/a n/a n/a 22:3n-3 ethyl ester

C22:6n3 Docosahexaenoic acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester

C24:0 Lignoceric acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester

C24:1n9 Nervonic acid n/a 277 n/a 2.76 one point n/a 22:3n-3 ethyl ester

Lab 11 C8:0 Caprylic (octanoic) 1.06 91.3 n/a n/a linear 1/x Caprylic-d3

C10:0 Capric (decanoic) 0.51 45.7 n/a n/a quadratic 1/x2 Capric-d3

C12:0 Lauric (dodecanoic) 1.00 84.0 n/a n/a quadratic 1/x2 Lauric-d3

C14:0 Myristic 10.8 1075 n/a n/a quadratic 1/x2 Myristic-d27

C14:1n5 Myristoleic 1.49 149 n/a n/a quadratic 1/x2 Myristic-d27

C15:0 Pentadecanoic 0.50 54.7 n/a n/a linear 1/x2 Pentadecanoic-d3

C16:1n7 Palmitoleic 25.3 2530 n/a n/a quadratic 1/x2 Palmitoleic-d14

C16:0 Palmitic 95.9 9591 n/a n/a quadratic 1/x2 Palmitic-d31

C17:0 Margaric (heptadecanoic) 2.85 52.2 n/a n/a quadratic 1/x Margaric-d3

C18:0 Stearic 27.2 2721 n/a n/a quadratic 1/x2 Stearic-d35

C18:1n9 Oleic 89.5 8949 n/a n/a linear 1/x2 13C-Oleic

C18:1n7 cis-Vaccenic 7.32 732 n/a n/a quadratic 1/x2 13C-Oleic

C18:2n6 Linoleic 86.4 8635 n/a n/a linear 1/x2 13C- Linoleic

C18:3n3 alpha- Linolenic 6.62 662 n/a n/a linear 1/x2 alpha -Linolenic-d14

C18:3n6 gamma- Linolenic 2.99 299 n/a n/a linear 1/x2 alpha -Linolenic-d14

C18:4n3 Stearidonic 0.49 50.3 n/a n/a quadratic 1/x Arachidic-d39

C20:0 Arachidic 0.95 95.1 n/a n/a linear 1/x2 Arachidic-d39

C20:1n9 11-Eicosenoic 1.02 102 n/a n/a quadratic 1/x2 Arachidic-d39

C20:2n6 11,14-Eicosadienoic 0.97 96.8 n/a n/a quadratic 1/x2 Arachidic-d39

C20:3n9 5Z, 8Z, 11Z-Eicosatrienoic 0.91 71.8 n/a n/a quadratic 1/x2 Arachidic-d39

C20:3n6 homo-gamma- Linolenic 4.63 463 n/a n/a quadratic 1/x2 alpha -Linolenic-d14

C20:4n6 Arachidonic 23.0 2300 n/a n/a linear 1/x2 Arachidonic-d8

C20:5n3 Eicosapentaenoic 3.97 397 n/a n/a quadratic 1/x2 Eicosapentaenoic-d5

C22:0 Docosanoic 1.94 194 n/a n/a linear 1/x2 Docosanoic-d4

C22:1n9 Docosenoic 0.56 56.1 n/a n/a quadratic 1/x Docosanoic-d4

C22:2n6 13,16- Docosadienoic 0.50 45.5 n/a n/a linear 1/x2 Docosanoic-d4

C22:4n6 Docosatetraenoic 1.55 155 n/a n/a quadratic 1/x2 Docosahexaenoic-d5

C22:5n3 Docosapentaenoic n-3 2.54 254 n/a n/a linear 1/x2 Docosahexaenoic-d5

C22:5n6 Docosapentaenoic n-6 1.50 150 n/a n/a linear 1/x2 Docosahexaenoic-d5

C22:6n3 Docosahexaenoic 8.06 806 n/a n/a linear 1/x2 Docosahexaenoic-d5

C23:0 Tricosanoic 0.83 47.2 n/a n/a linear 1/x2 Docosanoic-d4

C24:0 Tetracosanoic 1.40 140 n/a n/a linear 1/x2 Tetracosanoic-d4

C24:1n9 Nervonic 1.99 199 n/a n/a quadratic 1/x2 Tetracosanoic-d4

C26:0 Hexacosanoic (cerotic) 0.49 41.1 n/a n/a linear 1/x2 Tetracosanoic-d4
78

Appendix B

Participants in Interlaboratory Analytical Comparison Study of Total Fatty Acid Concentrations in Human 

Serum Exercise 01: QA12FASER01 (in alphabetical order)

Centers for Disease Control and Prevention (CDC)

Nutritional Biomarkers Branch

4770 Buford Hwy, NE

Atlanta, GA 30341

Contact: Rosemary Schleicher and Carissa Powers

Cornell University, 

Division of Nutritional Sciences

Savage Hall B38, 

Ithaca, NY 14850

Contact: Tom Brenna and Peter Lawrence

Hanyang Univeristy

Dept of Food and Nutrition 

College of Human Ecology 

222 Wangsimni-ro 

Seongdong-gu 

Seoul 133-791, Korea

Contact: Yongsoon Park and Sunyoung Jo

Lipid Analytical Labs 

University of Guelph Research Park

150 Research Lane, Room 100

Guelph, ON, Canada N1G 4T2

Contact: Jerry Piekarski and Bruce Holub

Mayo Clinic

Biochemical Genetics Laboratory Hilton 330

200 First Street S.W.

Rochester, MN 55905

Contact: Angela Tauscher
79

Minnesota Department of Health

Public Health Laboratory Division

MDA/MDH Lab Building

601 Robert St. N.

Saint Paul, MN 55155-2531

Contact: Tsutomu Shimotori

National Institutes of Health

LMBB, DICBR, NIAAA 

5625 Fishers Lane, Rm 3N01

Rockville, MD 20852

Contact: Yuhong Lin and Joseph Hibbeln

National Institute of Standards and Technology (NIST)

Chemical Sciences Division

100 Bureau Drive, MS 8392

Gaithersburg, MD 20899

Contact: Michele Schantz

OmegaQuant, LLC

2329 N. Career Ave, Suite 113

Sioux Falls, SD 57107

Contact: Jason Polreis

The University of Auckland 

School of Biological Science 

Room 110-285

3a Symonds Street 

Auckland 1010 NEW ZEALAND

Contact: Dang-Dung Nguyen

University of Waterloo

Dept. Kinesiology

200 University Ave West

Waterloo, ON N2L 5W7 Canada

Contact: Ken Stark