NISTIR 7340 

NIST Intercomparison Exercise 

Program for Organic Contaminants in 

the Marine Environment: 

Description and Results of 2005 Organic 

Intercomparison Exercises 

Michele M. Schantz 

John R. Kucklick 

Reenie M. Parris 

Dianne L. Poster 

Stephen A. Wise 
6 

NISTIR 7340 

NIST Intercomparison Program for 

Organic Contaminants in the 

Marine Environment: 

Description and Results of 2005 

Organic Intercomparison Exercises 

Michele M. Schantz, John R. Kucklick,1

 Reenie M. Parris, 

Dianne L. Poster, and Stephen A. Wise 

Analytical Chemistry Division 

National Institute of Standards and Technology 

Gaithersburg, MD 20899-8392 and 

1

Charleston, SC 29412 

 

September 2006 

U.S. DEPARTMENT OF COMMERCE

Carlos M. Gutierrez, Secretary

TECHNOLOGY ADMINISTRATION

Robert Cresanti, Under Secretary of Commerce for Technology

NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY

William A. Jeffrey, Director
6 

 Table of Contents

Abstract............................................................................................................................................7 

Introduction......................................................................................................................................8 

Sources and Preparation of Materials Used in 2005 Intercomparison Exercises ............................9 

Storage and Distribution of Materials..............................................................................................9 

Evaluation of Exercise Results ......................................................................................................10 

Establishment of the Assigned Values...............................................................................10 

Reported Results ............................................................................................................................11 

Performance Scores .......................................................................................................................12 

Accuracy Assessment (z-score) .........................................................................................12 

Precision Assessment (p-score) .........................................................................................13 

Discussion......................................................................................................................................13 

Acknowledgments..........................................................................................................................16 

Disclaimer and References ............................................................................................................16 

Tables.............................................................................................................................................18 

Table 1. Target analytes in NIST Intercomparison Exercise Program for 

Organic Contaminants in the Marine Environment ...............................................20 

Table 2. Mussel Tissue XII (QA05TIS12): Laboratory means of three replicates and 

exercise assigned values - TEO, and PAHs ...........................................................22 

Table 3. Mussel Tissue XII (QA05TIS12): Laboratory means of three replicates and 

exercise assigned values - Pesticides......................................................................23 

Table 4. Mussel Tissue XII (QA05TIS12): Laboratory means of three replicates 

and exercise assigned values - PCBs......................................................................24 

Table 5. Mussel Tissue XII (QA05TIS12): Laboratory means of three replicates 

and exercise assigned values - PBDEs...................................................................25 
6 

Table 6. SRM 2977: Laboratory means of three replicates 

and target values - TEO and PAHs ........................................................................26 

Table 7. SRM 2977: Laboratory means of three replicates 

and target values - Pesticides..................................................................................27 

Table 8. SRM 2977: Laboratory means of three replicates 

and target values - PCBs ........................................................................................28 

Table 9. SRM 2977: Laboratory means of three replicates 

and target values - PBDEs......................................................................................29 

Table 10. Marine Sediment XIII (QA05SED13): Laboratory means of three replicates and 

exercise assigned values - Water, TOC, and PAHs ...............................................30 

Table 11. Marine Sediment XIII (QA05SED13): Laboratory means of three replicates and 

exercise assigned values - Pesticides......................................................................31 

Table 12. Marine Sediment XIII (QA05SED13): Laboratory means of three replicates 

and exercise assigned values - PCBs......................................................................32 

Table 13. Marine Sediment XIII (QA05SED13): Laboratory means of three replicates 

and exercise assigned values - PBDEs...................................................................33 

Table 14. SRM 1941b: Laboratory means of three replicates 

and target values - Water, TOC, and PAHs ...........................................................34 

Table 15. SRM 1941b: Laboratory means of three replicates 

and target values - Pesticides..................................................................................35 

Table 16. SRM 1941b: Laboratory means of three replicates 

and target values - PCBs ........................................................................................36 

Table 17. SRM 1941b: Laboratory means of three replicates 

and target values - PBDEs......................................................................................37 

Table 18. Mussel Tissue XII (QA05TIS12): z-scores (25 %) by laboratory -TEO 

and PAHs................................................................................................................38 

Table 19. Mussel Tissue XII (QA05TIS12): z-scores (25 %) by laboratory - Pesticides......39 

Table 20. Mussel Tissue XII (QA05TIS12): z-scores (25 %) by laboratory - PCBs.............40 

Table 21. Mussel Tissue XII (QA05TIS12): z-scores (25 %) by laboratory - PBDEs ..........41 
6 

Table 22. Marine Sediment XIII (QA05SED13): z-scores (25 %) by laboratory - PAHs, 

Water, and TOC .....................................................................................................42 

Table 23. Marine Sediment XIII (QA05SED13): z-scores (25 %) by laboratory - 

 Pesticides................................................................................................................43 

Table 24. Marine Sediment XIII (QA05SED13): z-scores (25 %) by laboratory - PCBs .....44 

Table 25. Marine Sediment XIII (QA05SED13): z-scores (25 %) by laboratory - PBDEs...45 

Table 26. Mussel Tissue XII (QA05TIS12) and SRM 2977: RSDs for three replicates - 

TEO and PAHs.......................................................................................................46 

Table 27. Mussel Tissue XII (QA05TIS12) and SRM 2977: RSDs for three replicates - 

Pesticides................................................................................................................48 

Table 28. Mussel Tissue XII (QA05TIS12) and SRM 2977: RSDs for three replicates - 

PCBs.......................................................................................................................50 

Table 29. Mussel Tissue XII (QA05TIS12) and SRM 2977: RSDs for three replicates - 

PBDEs ....................................................................................................................52 

Table 30. Marine Sediment XIII (QA05SED13) and SRM 1941b: RSDs for three 

replicates - TEO and PAHs ....................................................................................54 

Table 31. Marine Sediment XIII (QA05SED13) and SRM 1941b: RSDs for three 

replicates - Pesticides .............................................................................................56 

Table 32. Marine Sediment XIII (QA05SED13) and SRM 1941b: RSDs for three 

replicates - PCBs ....................................................................................................58 

Table 33. Marine Sediment XIII (QA05SED13) and SRM 1941b: RSDs for three 

replicates - PBDEs..................................................................................................60 

Table 34. Comparison of Concentrations for Marine Sediment XIII (QA05SED13) 

and SRM 1941b......................................................................................................62 

Figure .............................................................................................................................................65 

Figure 1. Comparison of Concentrations for Mussel Tissue XII as determined in this study 

and in a CCQM pilot study for selected analytes...................................................65 
6 

Appendices

Appendix A: Description, Storage, Use, and Reporting Instructions 

 for Mussel Tissue XII (QA05TIS12)..........................................................................................66 

Appendix B: Description, Storage, Use, and Reporting Instructions 

 for Marine Sediment XIII (QA05SED13) ..................................................................................76 

Appendix C: Laboratory Notes Accompanying Data, Mussel Tissue XII ...................................86 

Appendix D: Laboratory Notes Accompanying Data, Marine Sediment XIII .............................89 

Appendix E: Laboratory Methods Used, Mussel Tissue XII........................................................93 

Appendix F: Laboratory Methods Used, Marine Sediment XIII................................................101 

Appendix G: Charts of Mussel Tissue XII and SRM 2977, Results by Analyte........................109 

Appendix H: Charts of Marine Sediment XIII and SRM 1941b, Results by Analyte................193 

Appendix I: Data Received after the first draft of this Report was Distributed .........................270 

Appendix J: List of Laboratories Participating in 2005 Intercomparison Exercises ...................281 
6 

Abstract

In support of marine monitoring measurement programs, the National Institute of Standards and 

Technology (NIST) conducts interlaboratory comparison exercises. The intercomparibility of 

data after participation in these exercises provides one mechanism for participating 

laboratories/monitoring programs to evaluate the quality and comparability of their performance 

in measuring selected organic contaminants in environmental samples. In this report, results of 

the 2005 exercises of the NIST Intercomparison Exercise Program for Organic Contaminants in 

the Marine Environment are described in which selected polycyclic aromatic hydrocarbons 

(PAHs), polychlorinated biphenyl (PCB) congeners, chlorinated pesticides, and polybrominated 

diphenyl ethers (PBDE) congeners were determined in Mussel Tissue XII and Marine Sediment 

XIII exercise materials. The analytical methods used by each participating laboratory in this 

performance-based program are also summarized. 
6 

Introduction

The preparation and distribution of two materials, Mussel Tissue XII (QA05TIS12) and Marine 

Sediment XIII (QA05SED13), used in interlaboratory comparison exercises in 2005 for the 

National Institute of Standards and Technology (NIST) Intercomparison Exercise Program for 

Organic Contaminants in the Marine Environment, and the results of these exercises are 

described in this report. The analytical methods used by each participating laboratory are also 

summarized. 

Tools and mechanisms for the assessment of data produced by laboratories providing 

environmental analyses are critical because decision-making based on inaccurate results or data 

of unknown quality can have significant economic and health consequences. NIST provides a 

variety of activities in support of environmental monitoring programs for organic contaminants. 

The largest of these programs was initiated and funded in part for 12 years (until 1999) by the 

National Oceanic and Atmospheric Administration (NOAA) National Status and Trends (NS&T) 

Marine Monitoring Program [1,2,3]. The Environmental Protection Agency (EPA) 

Environmental Monitoring and Assessment Program (EMAP) also participated in the 

NIST/NOAA NS&T effort for a number of years. Private sector and other laboratories that 

could not be accommodated under the NOAA, EPA, and NIST funding have reimbursed NIST 

for participation costs and have participated in these exercises and workshops as part of the NIST 

Intercomparison Exercise Program for Organic Contaminants in the Marine Environment. NIST 

is now continuing this program on a pay-to-participate basis. Through this program, NIST 

provides mechanisms for assessing the interlaboratory and temporal comparability of data with 

the goal of improving measurements for the monitoring of organic contaminants such as 

polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyl (PCB) congeners, 

chlorinated pesticides, and, as of the 2005 exercise, polybrominated diphenyl ethers (PBDE) 

congeners in bivalve, sediment, and fish samples. This program includes the development of 

improved analytical methods, production of needed NIST Standard Reference Materials (SRMs) 

and other control materials, conduct of annual interlaboratory comparison exercises, and the 

coordination of workshops to discuss the results of these exercises and to provide a forum for 

cooperative problem-solving efforts by participants. Current participants represent multilaboratory monitoring programs as well as a number of individual programs, and include federal, 

state/municipal, university/college, private sector, and international laboratories. In this 

performance-based program, each participating laboratory uses its current methods for analysis 

of similar materials for its program customers. 

For the annual intercomparison exercises, samples of two natural-matrix, homogeneous materials 

that are derived from the marine environment and that have not been fortified with any of the 

target analytes are analyzed by the participating laboratories. Typical materials, such as mussel 

or fish tissue homogenates or wetted marine sediment, have levels of target analytes in the 1 ng/g 

to 15000 ng/g range. The target analytes are listed in Table 1. 

Numerical indices, z- and p-scores, are used to assess and track laboratory performance for 

accuracy and precision, respectively, and to provide a mechanism for assessing the comparability 
6 

of data being produced by the participating laboratories for over 75 target analytes, total organic 

carbon (TOC), percent total extractable organics (TEO), and percent moisture. 

Sources and Preparation of Materials Used in 2005 Intercomparison Exercises 

Mussel Tissue XII. Mussel Tissue XII was prepared by freeze-drying approximately 5.5 kg of 

SRM 1974b Organics in Mussel Tissue (Mytilus edulis) [4]. Following freeze-drying, the bulk 

material (580 g) was radiation sterilized and then sieved through 25, 45, and 60 mesh sieves. 

The material that passed through the 60 mesh sieve (<250 μm) was then homogenized and 

bottled with approximately 8 g of freeze-dried tissue per bottle. As the Mussel Tissue XII 

material was a different sieved subset of the original SRM, it would not be expected to have the 

same concentrations of the analytes of interest as the original SRM. Each participant received 

one bottle. This freeze-dried mussel tissue homogenate material had not been enriched or 

spiked. 

Marine Sediment XIII. Marine Sediment XIII was prepared from SRM 2702 Inorganics in 

Marine Sediment. SRM 2702 was prepared from bulk dried sediment remaining from the 

preparation of SRM 1941b Organics in Marine Sediment [6]. However, the sediment bottled for 

SRM 2702 was sieved at 70 μm while the sediment bottled for SRM 1941b was sieved at 150 

μm. The Marine Sediment XIII material was issued as a wet sediment to more closely match the 

matrix of wet sediments routinely analyzed by the laboratories. A calibrated toploader balance 

(resolution of 0.01 g) was used for weighing the SRM 2702 sediment and water. For each 

sample, 11.00 g of SRM 2702 sediment (as received) was weighed into a tared 2-oz, wide-mouth 

bottle. The bottle was then capped and stored in the dark at room temperature. Approximately 

four days before samples were to be shipped to laboratories participating in the intercomparison 

exercise, 9.0 g of HPLC-grade water were added by pipet to each tared bottle of sediment. 

(Preliminary trials had shown that a minimum of 9 g of water would moisten 11 g of this 

sediment.) The mass of sediment and water in each bottle were recorded. Each sample was 

tilted by hand until no dry sediment was visible. Only a very small amount of water was 

observed on the top of the wet sediment. After being held 24 h at room temperature (in the 

dark), followed by approximately 4 h at -20 °C, each bottle of material was stored at -80 °C until 

shipped. The bottles were never inverted until the wet samples had been frozen in the bottom of 

the bottles. The material was not enriched or spiked with any of the analytes of interest in this 

intercomparison exercise. 

Storage and Distribution of Materials

Mussel Tissue XII material was stored at room temperature, and Marine Sediment XIII material 

was stored at -80 °C until shipped via overnight delivery to participating laboratories. 

Instructions for the storage and use of the exercise material and a diskette with files for electronic 

submission of data were included with each set of material shipped. These instructions are 

reproduced in Appendices A and B. 

Each laboratory participating in these intercomparison exercises was sent the following by 

overnight delivery: 
6 

Exercise 1: Mussel Tissue XII (QA05TIS12) 

One bottle of Mussel Tissue XII material (shipped on dry ice) 

Description of the materials and storage/use/reporting instructions for 

 the exercise (see Appendix A.) 

Files for the reporting of results were sent as an e-mail attachment. 

Exercise 2: Marine Sediment XIII (QA05SED13) 

Three bottles of Marine Sediment XIII material (shipped on dry ice) 

Description of the materials and storage/use/reporting instructions for 

 the exercise (see Appendix B.) 

Files for the reporting of results were sent as an e-mail attachment. 

In the an e-mail message sent notifying the participants of the sample shipment, each participant 

was asked to analyze each of three replicate samples (three from one bottle for the mussel tissue 

and one from each jar for the sediment) to provide a more realistic assessment of laboratory 

precision and, if possible, to concurrently analyze the NIST SRM 2977 Mussel Tissue [7] with 

Mussel Tissue XII and NIST SRM 1941b Organics in Marine Sediment [6] with Marine 

Sediment XIII. 

Evaluation of Exercise Results

Establishment of the Assigned Values

The following guidelines were used by the NIST exercise coordinators for the establishment of 

the exercise "Assigned Values" for these two exercises. Each laboratory’s performance on 

concurrent Standard Reference Material (SRM) analyses was used to determine if that 

laboratory’s results would be eligible for inclusion in the calculation of the exercise assigned 

value for the unknown material for a particular analyte. The results reported for the unknown 

materials from laboratories that did not report results for the SRMs were not used in these 

calculations. After the exercise assigned values, standard deviations, and 95% confidence limits 

had been calculated, all reported results for the Mussel Tissue XII and Marine Sediment XIII 

materials were evaluated relative to the exercise assigned values. 

Laboratory data submission: Each participating laboratory was to submit data from three 

replicate determinations of the “unknown” materials (Mussel Tissue XII and Marine Sediment 

XIII) and was requested to report results of concurrent analyses of NIST SRM 2977, a freezedried mussel tissue SRM, and SRM 1941b, a marine sediment SRM. Laboratories were 

requested to report these results to three significant figures and to provide brief descriptions of 

their extraction, cleanup, and analytical procedures. 

Determination of laboratory analyte means: For each laboratory, the laboratory analyte mean of 

the three sample results (S1, S2, and S3) was calculated for each analyte. Non-numerical data 

were treated as follows: A mean "<value" was used when three "<values" were reported; NA 

(not analyzed/determined) was used for three reported NA's; and, if the reported results were of 

mixed type, e.g., S1 and S2 were numerical values and S3 was reported as “<value”, the two 

similar "types" were used to either determine the mean or to set a non-numerical descriptor. 
6 

Determination of assigned values: The assigned values are the means of the acceptable data as 

defined here. For a particular analyte, the performance on the reference material was deemed 

acceptable for the purpose of this exercise if the laboratory result was within 30 % of the upper 

and lower limits of the confidence interval for analytes listed in the Certificates of Analysis for 

SRM 2977 and SRM 1941b. For each analyte of interest for which a certified value is not 

provided in these materials, a “target” concentration and the associated uncertainty were 

calculated. The targets for SRM 2977 were based on reference concentrations for SRM 2977. 

The targets for SRM 1941b were based on results of the 1999 exercise in which SRM 1941b was 

used as the unknown material and for the PBDEs on an interlaboratory study coordinated in 2004 

specifically for the determination of PBDE congeners in sediment [8]. Laboratory results within 

target upper and lower limits, typically 30 % to 40 %, of these concentrations were deemed 

acceptable for this exercise. If a laboratory demonstrated acceptable performance on a particular 

analyte in the reference material, that laboratory’s results for that analyte in the corresponding 

“unknown” exercise material was then used in the calculation of the analyte’s exercise assigned 

value, unless it was deemed an outlier. For evaluation of potential outliers, statistical tests and 

expert analyst judgement were used after viewing both normal and log normal plots of the data. 

This judgement utilized knowledge of potential coeluters based on the laboratory's reported 

methods. In instances in which the analyte concentration was below the detection limit of most 

participating laboratories, no exercise assigned value was calculated. In data sets where a 

number of laboratories report results as "not detected" at various detection limits, there is no 

consensus as to what numerical value should be assigned to these results in the computation of 

grand means, etc.; e.g., "0,” half Detection Limit (DL), and the DL value itself have all been 

used and the choice is influenced by the particular data set. 

Reported Results

Laboratories were assigned numerical identification codes in order of receipt of data with the 

exception of NIST, which is Laboratory 1 in these exercises. A laboratory was assigned the 

same code for each material. There are two results from NIST reported: 1a generated in the 

NIST Gaithersburg laboratory and 1c generated in the NIST Charleston laboratory. The 

laboratory mean replicate data are shown in Tables 2 to 5 and Tables 6 to 9 for the Mussel Tissue 

XII and SRM 2977, respectively, and in Tables 10 to 13 and 14 to 17 for Marine Sediment XIII 

and SRM 1941b, respectively. Included in the means tables for Mussel Tissue XII and Marine 

Sediment XIII are the exercise assigned values, the standard deviation of the assigned value, the 

percent relative standard deviation (% RSD), and the calculated 95 % confidence limit of the 

assigned value for the percent water (sediment), percent total extractable organics, TEO (mussel 

tissue), total organic carbon, TOC (sediment), PAHs, chlorinated pesticides, PCB congeners, and 

PBDE congeners. Notes included by a laboratory with its data are listed in Appendices C 

(Mussel Tissue XII) and D (Marine Sediment XIII). Summaries of the methods used by each 

laboratory are in Appendices E (Mussel Tissue XII) and F (Marine Sediment XIII). Tables 6 

through 9 and 14 through 17 summarize the data received from the participating laboratories for 

SRM 2977 and 1941b, respectively. The certified and target values for the analytes of interest 

are also shown in these tables. 
6 

In Appendices G (Mussel Tissue XII) and H (Marine Sediment XIII), charts of the mean 

numerical results reported by each laboratory for each analyte are shown for the exercise 

material and the corresponding reference material. 

Three laboratories reported data after the first draft of this report was distributed to the 

participants. The data from these laboratories are summarized in Appendix I but are not 

presented in the charts (Appendices G and H) and are not included in the calculation of the 

assigned values. 

Performance Scores

The exercise coordinators recognize that different programs have different data quality needs. 

The acceptability of the results submitted by a particular laboratory will be decided by the 

individual program(s) for which the laboratory provides data. Typically, the program will use 

these exercise results in conjunction with the laboratory's performance in the analysis of certified 

reference materials and/or control materials, and of other quality assurance samples. These 

exercise results are exhibited in a number of ways in this report to facilitate their use by these 

programs in their acceptability assessments. 

IUPAC guidelines [9] describe the use of z-scores and p-scores for assessment of accuracy and 

precision in intercomparison exercises such as those described in this report. These indices 

assess the difference between the result of the laboratory and the exercise assigned value and can 

be used, with caution, to compare performance on different analytes and on different materials. 

Accuracy Assessment (z-score)

z-score = (bias estimate)/(performance criterion) = (x - X)/ σ

where x is the individual laboratory result, X is the "Exercise Assigned Value,” and σ is the 

target value for standard deviation. 

As described in the IUPAC guidelines, the choice of σ is dependent upon data quality objectives 

of a particular program. It can be "fixed" and arrived at by perception, prescription, or reference 

to validated methodology (e.g., σ = 0.025 X; X is the exercise assigned value,), or it can be an 

estimate of the actual variation (e.g., the calculated sample standard deviation, s, from the 

exercise data). The "fixed" performance criterion is more useful in the comparison of a 

laboratory's performance on different materials while the use of the actual variation may be more 

useful within a given exercise, for example, if the determination of a particular analyte is 

exceptionally problematic. 

 

We have calculated and reported z-scores using the fixed performance criterion for each analyte 

for each laboratory. At a previous workshop, it was decided to use "25 % of the exercise 

assigned value" as the fixed target value for standard deviation for this program. The z-scores 

calculated for these exercises can thus be interpreted as shown in the following examples: 

z-score (25 % X): 

+1 ⇒ laboratory result is 25 % higher than the assigned value 
6 

-2 ⇒ laboratory result is 50 % lower than the assigned value. 

From a scientific point of view, IUPAC does not recommend the classification of z-scores but 

allows that a common classification is: 

z ≤ 2 Satisfactory 

2 < z < 3 Questionable 

z ≥ 3 Unsatisfactory. 

Tables 18 through 21 summarize the z-scores (25 %) for each laboratory for each reported 

analyte in Mussel Tissue XII while Tables 22 through 25 summarize the z-scores (25 %) for each 

laboratory for each reported analyte in Marine Sediment XIII. 

Precision Assessment (p-score)

 p-score = σlab / σtarget

Prior to the 1994 exercises, participating laboratories typically analyzed the three replicate 

samples for an exercise with the same sample set, i.e., one set of samples with the same blank, 

calibration curve, etc. applicable for each. Since the repeatability for replicates within a set 

generally shows better reproducibility than for replicates across different sets, this does not result 

in data that are very useful for realistic uncertainty assessment. Since 1994, laboratories have 

been requested to process each replicate in a different sample set for uncertainty assessment. 

For the calculation of p-scores for this program, the σ values used are coefficients of variation 

(CV calculated as relative standard deviations) with the current target σ (CV) for the three 

replicates being 15 %. 

Tables 26 through 29 summarize the relative standard deviations (RSDs) calculated from the 

three concentrations reported by the laboratory for each analyte quantified in Mussel Tissue XII 

while Tables 30 through 33 summarize the RSDs calculated for each reported analyte by 

laboratory in Marine Sediment XIII. To calculate the p-scores (15 %), divide the RSDs reported 

in the tables by 15%. If a different criterion is chosen, follow the same procedure, and divide the 

RSD by that criterion. 

 

Discussion

Laboratories were requested to quantify 26 PAHs, 25 chlorinated pesticides, 25 PCB congeners, 

and 34 PBDE congeners in this year’s exercise. A total of 12 sets of results were submitted for 

Mussel Tissue XII, and 11 sets of results were submitted for Marine Sediment XIII. In the 

mussel tissue exercise, one laboratory (12) reported data for SRM 2978 Mussel Tissue as the 

control material for the pesticides, and in the sediment exercise, one laboratory (2) reported data 

for SRM 1944 New York/New Jersey Waterway Sediment as the control material. Their data 

were evaluated based on the certified and target values for these SRMs (see Evaluation of 

Exercise Results above). 
6 

The concentrations of the PAHs of interest in Mussel Tissue XII range from 2 ng/g dry-mass 

basis to 200 ng/g dry-mass basis, the concentrations of the pesticides of interest range from < 1 

ng/g dry-mass basis to 35 ng/g dry-mass basis, and the concentrations of the PCB congeners 

range from < 1 ng/g dry-mass basis to 90 ng/g dry-mass basis. For the chlorinated pesticides, 9 

of the 25 compounds were above the detection limits for the majority of the laboratories 

reporting, while 22 of the 25 PCB congeners were above the detection limits for the majority of 

the laboratories. There was poor agreement among the laboratories for total extractable organics 

(TEO), ranging from 0.3 % to 15.0 % even though the laboratories are reporting using similar 

methods for determining the TEOs (Appendix E). TEO is sometimes referred to as percent lipid 

but is typically determined by taking a know portion of the extract and evaporating to dryness 

and then weighing the dried residue. As one can imagine, the TEO value is then dependent on 

the extraction method and solvent used and the drying method used. It is, therefore typical to see 

the TEO values vary greatly from lab to lab particularly for relatively lean (non-fatty) materials. 

Some of the bottles prepared as Mussel Tissue XII were labeled for use in a separate 

interlaboratory study as part of the Organic Working Group of the Comité Consultative pour la 

Quantité de Matière (CCQM). Seven National Metrology Institutes (NMIs) or designated NMIs 

participated in this study for a limited number of analytes. The exercise means and standard 

deviations are shown in Figure 1 and compared to the exercise assigned means and standard 

deviations from this study. The means from the two studies agreed within the uncertainties of 

the data for this limited analyte set. 

The z-scores for the PAHs, pesticides, PCB congeners, and PBDE congeners in Mussel Tissue 

XII based on 25 % of the exercise assigned value are summarized in Tables 18 to 21, 

respectively. The majority of the z-scores based on 25 % are within ± 2 (± 50 % of the exercise 

assigned value). The RSDs for Mussel Tissue XII are summarized in Tables 26 to 29 for the 

PAHs, pesticides, PCB congeners, and PBDE congeners, respectively. Only five laboratories 

reported results for a limited number of PBDE congeners; however, for some of the congeners 

the agreement among the laboratories reporting is good particularly considering the low 

concentrations. 

The PAH concentrations in Marine Sediment XIII range from 25 ng/g dry-mass basis to 800 ng/g 

dry-mass basis. The pesticide concentrations range from below the detection limits of the 

methods used to 4.5 ng/g dry-mass basis, while the PCB concentrations range from <1 ng/g drymass basis to 5 ng/g dry-mass basis. There was good agreement among the laboratories for 

percent water in the wet sediment. Only four laboratories returned data for the TOC with the 

values ranging from 1.8 % to 3.0 %. 

As discussed above, the material used for Marine Sediment XIII, SRM 2702, was prepared from 

the same bulk sediment as SRM 1941b with the difference in the sieve fraction used. SRM 2702 

used the material <70 μm while SRM 1941b used the material <150 μm. The concentrations 

determined in this study for Marine Sediment XIII are compared to the certified and reference 

concentrations for SRM 1941b in Table 34. For the majority of the analytes, the concentrations 

in Marine Sediment XIII (SRM 2702) are lower than those in SRM 1941b although the 

difference between the two materials is not consistent across all of the analytes even within the 

PAHs, PCBs, and pesticides. 
6 

The z-scores for the PAHs, pesticides, PCB congeners, and PBDE congeners based on 25 % of 

the exercise assigned value are summarized for Marine Sediment XIII in Tables 22 to 25, 

respectively. In general, the z-scores based on 25 % were within ± 2 (± 50 % of the exercise 

assigned value) for Marine Sediment XIII. The RSDs for the Marine Sediment XIII are 

summarized in Tables 30 to 33 for the PAHs, pesticides, PCB congeners, and PBDE congeners, 

respectively. As for the Mussel Tissue XI, only five laboratories reported data for the PBDE 

congeners. 

As in the past exercises, a variety of methods were used for extraction, extract cleanup, and 

analysis. These are summarized in Appendix E for the mussel tissue and Appendix F for the 

marine sediment. For the PAHs in the mussel tissue and marine sediment, all of the laboratories 

used gas chromatography with mass spectrometry (GC/MS). For the chlorinated analytes in the 

mussel tissue, laboratories 8, 11, and 12 specified the use of high-resolution MS, and laboratories 

5, 9, and 10 used GC-ECD for the PCB congeners and 5, 6, 9, and 10 for the pesticides. For the 

PBDE congeners, laboratories 7, 8, and 12 used GC with high-resolution MS while laboratory 1c 

used GC with low-resolution MS in the negative chemical ionization mode and laboratory 4 used 

GC with low-resolution MS in the electron ionization mode. There was no obvious correlation 

between z-scores and method used. 

For the 2005 exercises, the data provided in the various figures and tables of this report can be 

used for assessing the comparability of results of over 100 analytes of interest in this program 

and the performance of individual laboratories. In these exercises, interlaboratory variability is a 

greater contributor to measurement incomparability than intralaboratory variability. 

Subgroups of the exercise participants have demonstrated comparability of results for many 

analytes within the 0 to 2 z-range based on use of 25 % of the exercise assigned concentration as 

the performance criterion. This implies that this subgroup can distinguish between two samples 

that have an analyte concentration difference of 100 %. The reported accuracy and 

reproducibility indices (z- and p-scores, respectively) can be easily converted to conform to the 

acceptability requirements of a particular program. For example, a z-score based on 25 % can be 

multiplied by two to convert to a z-score based on 12.5 % of the analyte concentration. 

It is important to evaluate the non-quantitative results reported by each laboratory as well. 

Although these results are not easily presented or numerically evaluated, they are included in the 

various tables of this report that list the mean and individual results of the laboratories. The 

laboratory and its data users should closely examine these non-quantitative results. Decisions 

based on false negative or false positive results from a laboratory can lead to significant 

environmental and/or economic consequences. Some laboratories reported detection limits in 

these “real” matrix materials that may be too high for the data quality needs of their program(s), 

and these issues should be assessed as well. 

Intercomparison exercises provide an important mechanism for assessing the comparability, 

accuracy, precision, and reproducibility of data being produced by the participating laboratories. 

Exercise materials similar in matrix, form, and analyte concentration to typical samples routinely 
6 

analyzed by the laboratories are most useful for demonstrating the level of comparability and for 

revealing potential problem areas. 

For the determination of the target compounds in these complex marine matrices with relatively 

low concentrations of these analytes, the levels of bias and reproducibility of many of the 

participating laboratories meet their current acceptability requirements; however, there is 

certainly room for improvement. Minimizing the among-laboratory biases so that the analytical 

variability is significantly less than the field sampling variability should be an achievable goal. 

Acknowledgments

The time and effort of the analysts and management of the participating laboratories and the 

assistance of the NIST Standard Reference Materials Program with the procurement and 

preparation of the exercise materials are gratefully acknowledged. 

Disclaimer 

Certain commercial equipment, instruments, or materials are identified in this report to specify 

adequately the experimental procedure. Such identification does not imply recommendation or 

endorsement by the National Institute of Standards and Technology, nor does it imply that the 

materials or equipment identified are the best available for the purpose. 

References

1. A. Y. Cantillo and R. M. Parris, “Evaluation of Trace Organic NOAA Status and Trends 

Quality Assurance Project Performance,” in Quality Assurance for Analytical 

Laboratories, M. Parkany (ed.), Royal Society of Chemistry, Spec. Publ. No. 130 (1993). 

2. A. Y. Cantillo and R. M. Parris, National Status and Trends Program Quality Assurance 

Project: Trace Organic Intercomparison Exercise Results 1986-1990, NOAA Tech. 

Memo. NOS/ORCA 69, Silver Spring, MD (1994). 

3. A. Y. Cantillo, NS&T Quality Assurance Project Intercomparison Exercise Results 1991-

1993, NOAA Tech. Memo. NOS/ORCA 79, Silver Spring, MD (1995). 

4. Certificate of Analysis for Standard Reference Material (SRM) 1974b Organics in 

Mussel Tissue (Mytilus edulis), National Institute of Standards and Technology (NIST), 

Gaithersburg, MD, 2003. https://srmors.nist.gov/view_detail.cfm?srm=1974B

5. Certificate of Analysis for Standard Reference Material (SRM) 2702 Inorganics in 

Marine Sediment, National Institute of Standards and Technology (NIST), Gaithersburg, 

MD, 2004. https://srmors.nist.gov/view_detail.cfm?srm=2702
6 

6. Certificate of Analysis for Standard Reference Material (SRM) 1941b Organics in 

Marine Sediment, National Institute of Standards and Technology (NIST), Gaithersburg, 

MD, 2002. https://srmors.nist.gov/view_detail.cfm?srm=1941B

7. Certificate of Analysis for Standard Reference Material (SRM) 2977 Mussel Tissue 

(Organic Contaminants and Trace Elements), National Institute of Standards and 

Technology (NIST), Gaithersburg, MD, 2000. 

https://srmors.nist.gov/view_detail.cfm?srm=2977

8. Stapleton, H.M., Keller, J.M., Schantz, M.M., Kucklick, J.R., and Wise, S.A., NIST 

Intercomparison Exercise Program for Polybrominated Diphenyl Ethers (PBDEs) in 

Marine Sediment: Description and Results of the 2004 Intercomparison Exercise, 

NISTIR 7278, Gaithersburg, MD (2005). 

9. IUPAC “The International Harmonized Protocol for the Proficiency Testing of 

(Chemical) Analytical Laboratories,” Pure Appl. Chem. 65 (9), 2123-2144 (1993). 
6 

List of Tables

Table 1. Target Analytes in NIST Intercomparison Exercise Program for Organic 

Contaminants in the Marine Environment. 

Table 2. Mussel Tissue XII (QA05TIS12): Laboratory means of three replicates and exercise 

assigned values – TEO and PAHs 

Table 3. Mussel Tissue XII (QA05TIS12): Laboratory means of three replicates and exercise 

assigned values – Pesticides 

Table 4. Mussel Tissue XII (QA05TIS12): Laboratory means of three replicates and exercise 

assigned values – PCBs. 

Table 5. Mussel Tissue XII (QA05TIS12): Laboratory means of three replicates and exercise 

assigned values – PBDEs. 

Table 6. SRM 2977: Laboratory means of three replicates and target values – TEO and PAHs. 

Table 7. SRM 2977: Laboratory means of three replicates and target values – Pesticides. 

Table 8. SRM 2977: Laboratory means of three replicates and target values – PCBs. 

Table 9. SRM 2977: Laboratory means of three replicates and target values – PBDEs 

Table 10. Marine Sediment XIII (QA05SED13): Laboratory means of three replicates and 

exercise assigned values –Water, TOC, and PAHs 

Table 11. Marine Sediment XIII (QA05SED13): Laboratory means of three replicates and 

exercise assigned values – Pesticides 

Table 12. Marine Sediment XIII (QA05SED13): Laboratory means of three replicates and 

exercise assigned values – PCBs 

Table 13. Marine Sediment XIII (QA05SED13): Laboratory means of three replicates and 

exercise assigned values – PBDEs 

Table 14. SRM 1941b: Laboratory means of three replicates and target values – Water, TOC, 

and PAHs. 

Table 15. SRM 1941b: Laboratory means of three replicates and target values – Pesticides. 

Table 16. SRM 1941b: Laboratory means of three replicates and target values – PCBs. 

Table 17. SRM 1941b: Laboratory means of three replicates and target values – PBDEs. 
6 

Table 18. Mussel Tissue XII (QA05TIS12): z-scores (25 %) by laboratory – TEO and PAHs 

Table 19. Mussel Tissue XII (QA05TIS12): z-scores (25 %) by laboratory – Pesticides 

Table 20. Mussel Tissue XII (QA05TIS12): z-scores (25 %) by laboratory – PCBs 

Table 21. Mussel Tissue XII (QA05TIS12): z-scores (25 %) by laboratory – PBDEs 

Table 22. Marine Sediment XIII (QA05SED13): z-scores (25 %) by laboratory – PAHs, Water, 

and TOC 

Table 23. Marine Sediment XIII (QA05SED13): z-scores (25 %) by laboratory – Pesticides 

Table 24. Marine Sediment XIII (QA05SED13): z-scores (25 %) by laboratory – PCBs 

Table 25. Marine Sediment XIII (QA05SED13): z-scores (25 %) by laboratory – PBDEs 

Table 26. Mussel Tissue XII and SRM 2977: RSDs for three replicates – TEO and PAHs 

Table 27. Mussel Tissue XII and SRM 2977: RSDs for three replicates – Pesticides 

Table 28. Mussel Tissue XII and SRM 2977: RSDs for three replicates – PCBs 

Table 29. Mussel Tissue XII and SRM 2977: RSDs for three replicates – PBDEs 

Table 30. Marine Sediment XIII and SRM 1941b: RSDs for three replicates – Water, TOC, and 

PAHs 

Table 31. Marine Sediment XIII and SRM 1941b: RSDs for three replicates – Pesticides 

Table 32. Marine Sediment XIII and SRM 1941b: RSDs for three replicates – PCBs 

Table 33. Marine Sediment XIII and SRM 1941b: RSDs for three replicates – PBDEs 

Table 34. Comparison of concentrations for Marine Sediment XIII and SRM 1941b 
6 

Table 1. Target Analytes in NIST Intercomparison Exercise Program for Organic 

Contaminants in the Marine Environment

Chlorinated Pesticides 

hexachlorobenzene 2,4'-DDE 

alpha-HCH (alpha-BHC) 4,4'-DDE 

gamma-HCH (gamma-BHC, Lindane) 2,4'-DDD 

beta-HCH 4,4'-DDD 

heptachlor 2,4'-DDT 

heptachlor epoxide 4,4'-DDT 

cis-chlordane (alpha-chlordane) aldrin 

trans-chlordane (gamma-chlordane) dieldrin 

oxychlordane endrin 

cis-nonachlor endosulfan sulfate 

trans-nonachlor endosulfan I 

mirex endosulfan II 

chlorpyrifos 

Polychlorinated Biphenyl Congeners

 PCB No. Compound Name

 8 2,4'-dichlorobiphenyl 

 18 2,2',5-trichlorobiphenyl 

 28 2,4,4'-trichlorobiphenyl 

 31 2,4',5-trichlorobiphenyl 

 44 2,2',3,5'-tetrachlorobiphenyl 

 49 2,2',4,5'-tetrachlorobiphenyl 

 52 2,2',5,5'-tetrachlorobiphenyl 

 66 2,3',4,4'-tetrachlorobiphenyl 

 95 2,2',3,5',6-pentachlorobiphenyl 

 99 2,2',4,4',5-pentachlorobiphenyl 

 101 2,2',4,5,5'-pentachlorobiphenyl 

 105 2,3,3',4,4'-pentachlorobiphenyl 

 118 2,3',4,4',5-pentachlorobiphenyl 

 128 2,2',3,3',4,4'-hexachlorobiphenyl 

 138 2,2',3,4,4',5'-hexachlorobiphenyl 

 149 2,2',3,4',5',6-hexachlorobiphenyl 

 153 2,2',4,4',5,5'-hexachlorobiphenyl 

 156 2,3,3',4,4',5-hexachlorobiphenyl 

 170 2,2',3,3',4,4',5-heptachlorobiphenyl 

 180 2,2',3,4,4',5,5'-heptachlorobiphenyl 

 187 2,2',3,4',5,5',6-heptachlorobiphenyl 

 194 2,2',3,3',4,4',5,5'-octachlorobiphenyl 

 195 2,2',3,3',4,4',5,6-octachlorobiphenyl 

 206 2,2',3,3',4,4',5,5',6-nonachlorobiphenyl 

 209 decachlorobiphenyl 

Table 1. (continued) 
6 

Polycyclic aromatic hydrocarbons (PAH)

naphthalene benz[a]anthracene 

2-methylnaphthalene chrysene 

1-methylnaphthalene triphenylene 

biphenyl benzo[b]fluoranthene 

2,6-dimethylnaphthalene benzo[j]fluoranthene 

acenaphthylene benzo[k]fluoranthene 

acenaphthene benzo[e]pyrene 

1,6,7-trimethylnaphthalene benzo[a]pyrene 

fluorene perylene 

phenanthrene indeno[1,2,3-cd]pyrene 

anthracene dibenz[a,h]anthracene 

1-methylphenanthrene benzo[ghi]perylene 

fluoranthene 

pyrene

Polybrominated diphenyl ethers (PBDEs)

 BDE 15 (4,4’-dibromo-) BDE 138 (2,2’,3,4,4’,5’-hexabromo-) 

 BDE 17 (2,2’,4-tribromo-) BDE 153 (2,2’,4,4’,5,5’-hexabromo-) 

 BDE 25 (2,3’,4-tribromo-) BDE 154 (2,2’,4,4’,5,6’-hexabromo-) 

 BDE 28 (2,4,4’-tribromo-) BDE 155 (2,2’,4,4’,6,6’-hexabromo-) 

 BDE 30 (2,4,6-tribromo-) BDE 156 (2,3,3’,4,4’,5-hexabromo-) 

 BDE 33 (2’,3,4-tribromo-) BDE 181 (2,2’,3,4,4’,5,6-heptabromo-) 

 BDE 47 (2,2’,4,4’-tetrabromo-) BDE 183 (2,2’,3,4,4’,5’,6-heptabromo-) 

 BDE 49 (2,2’,4,5’-tetrabromo-) BDE 190 (2,3,3’,4,4’,5,6-heptabromo-) 

 BDE 66 (2,3’,4,4’-tetrabromo-) BDE 191 (2,3,3’,4,4’,5,6’-heptabromo-) 

 BDE 71 (2,3’,4’,6-tetrabromo-) BDE 196 (2,2’,3,3’,4,4’,5,6’-octabromo-) 

 BDE 75 (2,4,4’,6-tetrabromo-) BDE 197 (2,2’,3,3’,4,4’,6,6’-octabbromo-) 

 BDE 85 (2,2’,3,4,4’-pentabromo-) BDE 203 (2,2’,3,4,4’,5,5’,6-octabromo-) 

 BDE 99 (2,2’,4,4’,5-pentabromo-) BDE 205 (2,3,3’,4,4’,5,5’,6-octabromo-) 

 BDE 100 (2,2’,4,4’,6-pentabromo-) BDE 206 (2,2’,3,3’,4,4’,5,6,6’-nonabromo-) 

 BDE 116 (2,3,4,5,6-pentabromo-) BDE 207 (2,2’3,3’,4,4’,5,6,6’-nonabromo-) 

 BDE 118 (2,3’,4,4’,5-pentabromo-) BDE 208 (2,2’,3,3’,4,5,5’,6,6’-nonabromo-) 

 BDE 119 (2,3’,4,4’,6-pentabromo-) BDE 209 (decabromo-)
Table 2. Mussel TissueXII (QA05TIS12): Laboratory means of three replicates and exercise assigned values - TEO and PAHs 

 (reported as if three figures were significant)

Laboratory No. 1a 1c 3 4 5 6 7 8 9 10 11 12 Value s %RSD

TEO (percent) NA 4.13 NA NA 1.93 15.0 0.295 1.68 4.30 1.69 1.45 NA 3.81 4.73 124.1PAHs (ng/g dry mass) Exercise Assigned

Laboratory No. 1a 1c 3 4 5 6 7 8 9 10 11 12 Value s %RSD

naphthalene 9.87 8.18 8.76 5.61 12.5 43.5 14.2 21.6 2.52 103 NA NA 9.86 6.07 61.5

2-methylnaphthalene 4.77 2.05 3.19 3.82 <10 14.1 13.3 9.88 1.10 NA NA NA 8.00 5.16 64.5

1-methylnaphthalene 4.47 0.763 4.28 1.81 <8 6.96 4.15 NA 0.692 NA NA NA 3.66 2.44 66.6

biphenyl 2.87 2.02 2.11 1.81 <7 4.40 <0.7 NA 0.745 38.0 NA NA 1.91 0.77 40.0

2,6-dimethylnaphthalene coelution <5 5.55 1.78 <8 8.24 <0.8 NA 3.10 NA NA <3 4.67 2.85 61.0acenaphthylene <2 8.45 2.93 0.491 <11 3.64 3.75 <12.5 1.94 3.02 NA 5.50 3.72 2.40 64.6acenaphthene 2.39 1.44 6.48 0.896 <8 <0.78 3.42 <12.5 3.67 43.5 NA <2 2.93 2.21 75.41,6,7-trimethylnaphthalene coelution 6.44 NA 4.21 NA 5.72 NA NA 2.74 NA NA coelution 4.78 1.65 34.5fluorene 3.90 5.57 3.54 2.44 <7 4.46 6.50 <12.5 1.25 5.95 NA 3.40 3.64 1.64 45.1

phenanthrene 101 105 78.5 107 88.5 82.8 92.1 93.9 48.3 115 NA 96.5 88.7 17.9 20.2anthracene 4.75 9.72 7.78 2.87 <10 4.32 2.60 <12.5 3.39 39.0 NA 10.9 5.79 3.24 55.9

1-methylphenanthrene 111 113 78.5 122 104 73.9 79.6 NA 54.0 43.4 NA 118 89.7 27.7 30.9fluoranthene 165 150 119 172 129 127 119 146 69.7 253 NA 144 133 30 22.9

pyrene 213 210 177 241 179 209 172 210 100 352 NA 207 190 40 21.1benz[a]anthracene 35.9 29.6 19.8 31.5 23.8 21.4 22.6 25.9 11.9 NA NA 26.1 24.7 7.1 28.6chrysene coelution 48.0 81.3 coelution 94.5 106 46.9 110 48.9 coelution NA coelution 63.9 22.4 35.1

triphenylene coelution 49.2 NA NA NA NA NA NA NA coelution NA coelution no target

benzo[b ]fluoranthene 53.5 coelution 56.7 52.0 52.9 39.0 56.9 64.6 22.5 162 NA coelution 47.6 12.6 26.5benzo[j]fluoranthene 13.4 coelution NA NA NA NA NA NA 16.8 NA NA coelution no targetbenzo[k ]fluoranthene 17.3 12.7 17.8 coelution 31.3 33.1 17.5 37.3 NA 43.1 NA 30.7 16.3 2.4 15.0benzo[e]pyrene 82.5 85.8 68.9 86.6 77.1 75.3 68.7 66.6 41.7 166 NA 94.0 74.7 14.6 19.6

benzo[a]pyrene 8.66 12.2 4.66 7.26 8.86 7.72 6.55 <12.5 2.94 144 NA 6.36 7.25 2.65 36.6

perylene 4.26 4.74 <14.0 3.57 <5 17.1 NA NA 1.47 26.2 NA NA 3.51 1.44 41.2

indeno[1,2,3-cd]pyrene 12.2 coelution 11.0 15.7 19.5 12.0 15.7 26.4 5.15 23.3 NA 14.8 15.1 6.4 42.4

dibenz[a,h]anthracene coelution coelution 3.35 coelution <11 <0.78 <0.7 <12.5 2.08 5.31 NA coelution no target

benzo[ghi]perylene 23.3 25.9 19.3 28.5 23.9 23.1 34.9 43.1 11.5 21.9 NA 28.8 24.6 6.4 25.8Note: Bolded values were not used in the calculation of the exercise assigned value; NA = not analyzed
6 

Table 3. Mussel Tissue XII (QA05TIS12): Laboratory means of three replicates and exercise assigned values - Pesticides (reported as if three figures were significant)ng/g dry mass Exercise Assigned

Laboratory No. 1a 1c 3 4 5 6 7 8 9 10 11 12 Value s %RSD

alpha-HCH (a-BHC) <2 <1 NA <1.05 <2.0 <1.8 <2.5 NA <0.565 1.87 NA 0.247 no targethexachlorobenzene <2 <1 1.51 <0.996 <2.5 <1.8 <2.5 NA <0.355 NA NA 0.131 no targetgamma-HCH (g-BHC,lindane) <2 <1 <2.72 4.08 <1.5 <1.8 <2.5 NA <0.355 0.079 NA 0.094 no target

beta-HCH (b-BHC) <2 <1 NA 1.37 NA 2.50 <2.5 NA <0.301 0.351 NA <0.04 no targetheptachlor <2 <5 <2.72 <1.00 <2.0 <1.8 <2.5 NA <0.419 2.36 NA 0.226 no targetaldrin <2 <1 <2.72 <1.00 1.63 <1.8 <4 NA <0.428 0.658 NA <0.03 no targetheptachlor epoxide <2 <1 <2.72 <1.00 <2.0 <1.8 <5 NA <0.465 0.774 NA 0.297 no targetoxychlordane <2 <1 40.8 4.15 NA <1.8 <5 NA <0.52 2.89 NA 0.41057 no targetgamma-chlordane 8.47 <1 7.22 8.43 5.80 8.30 6.59 NA 7.97 6.32 NA 7.99 7.45 1.00 13.52,4'-DDE <2 <1 <2.72 1.43 <1.0 <1.8 <0.5 NA <1.28 8.42 NA 1.15 no targetendosulfan I <2 <1 <2.72 <2.63 <1.5 24.3 <5 NA <1.35 0.112 NA <0.2 no targetcis-chlordane (alpha-chlordane 16.8 17.8 9.39 11.0 8.43 11.0 12.1 NA 5.98 5.40 NA 10.5 12.1 3.4 27.9

trans-nonachlor 9.43 9.22 7.94 9.92 9.17 10.0 6.40 NA 5.47 5.10 NA 9.84 9.00 1.24 13.8dieldrin <5 <5 <2.72 3.51 7.23 14.2 9.09 NA 3.28 1.37 NA 2.94 6.70 4.42 65.9

4,4'-DDE 41.1 46.9 27.6 34.7 23.0 30.5 25.9 NA 18.4 29.6 NA 30.8 33.9 7.6 22.42,4'-DDD 5.61 10.8 7.11 14.4 7.70 8.12 6.05 NA 5.69 coelution NA 6.93 8.04 2.86 35.5endrin <2 <1 <2.72 NA <2.0 <1.8 <4 NA <0.502 coelution NA <0.07 no targetendosulfan II <2 <1 <2.72 NA <3.4 <1.8 <5 NA <0.392 0.130 NA <0.4 no target4,4'-DDD 16.4 27.6 20.6 42.8 18.3 <1.8 14.8 NA 14.5 15.1 NA 30.0 21.7 10.8 49.6

2,4'-DDT <2 <1 <2.72 1.85 <3.0 <1.8 <2.5 NA <0.547 7.59 NA 0.664 no target

cis-nonachlor 5.31 4.59 3.11 4.33 NA 3.84 3.86 NA 3.72 5.47 NA 4.22 4.27 0.76 17.84,4'-DDT <2 <1 <2.72 1.22 2.50 10.2 <2 NA 1.15 4.58 NA 1.85 1.68 0.63 37.6mirex <2 <1 <2.72 3.89 <1.5 <1.8 <1.5 NA <0.31 NA NA 0.421 no targetendosulfan sulfate <2 <1 NA NA NA <1.8 <4 NA <0.775 NA NA <0.2 no targetchlorpyrifos <2 NA NA NA NA <1.8 NA NA <0.401 NA NA NA no targetNote: Bolded values were not used in the calculation of the exercise assigned value; NA = not analyzed
6 

Table 4. Mussel Tissue XII (QA05TIS12): Laboratory means of three replicates and exercise assigned values - PCBs (reported as if three figures were significant)ng/g dry mass Exercise Assigned

Laboratory No. 1a 1c 3 4 5 6 7 8 9 10 11 12 Value s %RSD

PCB 8 2.22 2.76 1.74 NA <2.8 0.300 2.15 3.54 1.53 11.9 1.81 2.14 2.56 0.60 23.5

PCB 18 8.38 8.78 4.29 7.84 4.00 0.897 5.46 4.40 4.05 6.76 4.93 5.03 5.71 1.87 32.8PCB 28 30.0 27.6 19.8 24.3 20.4 2.81 21.0 24.8 14.2 coelution 23.1 26.9 23.2 4.6 19.7

PCB 31 22.1 26.2 NA 21.0 NA 2.52 16.7 21.3 NA coelution 16.6 22.9 21.7 3.1 14.2

PCB 44 38.8 37.3 21.9 33.8 20.2 3.73 24.1 23.4 16.8 19.1 45.3 50.4 31.4 11.2 35.7

PCB 49 49.7 52.3 NA 47.1 NA 5.53 27.9 41.5 23.9 21.4 34.4 36.5 37.2 11.3 30.4

PCB 52 57.0 66.6 38.7 55.1 37.7 6.32 42.0 40.7 31.6 22.3 45.0 51.5 46.6 10.7 22.9

PCB 66 52.8 57.4 43.9 51.5 74.0 6.82 33.3 39.1 30.7 50.7 42.0 57.1 48.4 12.4 25.6

PCB 95 59.4 64.7 NA 54.4 NA 4.68 36.9 47.0 NA 16.4 46.4 51.4 51.5 9.2 17.8

PCB 99 47.6 43.7 NA 48.0 NA 5.51 32.9 49.1 NA 14.9 55.5 52.1 47.0 7.2 15.4

PCB 101 97.7 113 82.9 85.5 98.5 13.1 70.0 66.3 52.2 26.1 89.1 90.4 88.1 14.4 16.3PCB 105 34.9 36.5 25.6 34.3 37.1 4.39 25.6 31.8 21.3 9.80 31.6 32.7 31.1 5.3 16.9

PCB 118 86.3 79.7 84.7 93.3 101 12.0 51.1 79.7 54.1 28.5 79.6 89.4 79.9 15.9 19.9

PCB 128 14.5 14.7 15.0 15.8 13.3 2.24 9.21 11.0 8.36 4.57 11.5 12.0 13.0 2.2 17.0PCB 138 coelution 61.7 68.8 coelution 91.8 11.3 57.8 coelution 50.8 54.0 coelution coelution 64.1 14.9 23.3

PCB 149 66.4 80.7 NA 57.6 NA 6.28 47.6 57.5 NA 26.9 50.4 59.3 61.5 11.2 18.2

PCB 153 coelution 147 72.3 123 86.3 11.4 52.1 60.6 63.9 71.9 82.6 97.3 85.7 29.6 34.5

PCB 156 6.80 5.19 NA 6.17 NA 0.713 4.07 4.03 NA NA 6.04 7.17 5.64 1.25 22.2PCB 170 1.82 1.46 1.38 1.69 2.20 0.390 <2 1.98 <0.729 2.53 1.63 1.50 1.80 0.38 21.0PCB 180 8.23 7.10 6.88 10.3 6.90 1.00 9.28 6.64 15.7 12.7 coelution coelution 9.29 3.11 33.5

PCB 187 19.9 19.3 15.9 21.9 17.9 2.84 15.2 14.0 12.6 14.2 24.9 22.0 18.0 4.0 22.0

PCB 194 <2 0.593 NA 0.535 NA <0.5 <2 0.406 NA 0.553 0.307 0.417 0.501 0.084 16.8

PCB 195 <2 <1 <2.72 <1.01 <1.8 <0.5 <2 <0.400 <0.265 0.687 0.050 0.0686 no targetPCB 206 <2 <1 <2.72 <1.02 <1.7 <0.5 <2.2 <0.400 <0.256 0.220 0.040 0.050 no targetPCB 209 <2 <1 <2.72 <1.01 <1.7 <0.5 <2.2 <0.400 <0.419 0.111 0.054 0.060 no targetNote: Bolded values were not used in the calculation of the exercise assigned value; NA = not analyzed
6 

Table 5. Mussel Tissue XII (QA05TIS12): Laboratory means of three replicates and exercise assigned values - PBDEs (reported as if three figures were significant)ng/g dry mass Exercise Assigned

1a 1c 3 4 5 6 7 8 9 10 11 12 Value s %RSD

BDE 15 NA <1 NA NA NA NA NA <2.00 NA NA NA 0.160 no targetBDE 17 NA 2.98 NA NA NA NA NA 4.26 NA NA NA 3.92 3.72 0.66 17.8BDE 25 NA <1 NA NA NA NA NA 4.26 NA NA NA other no targetBDE 28 NA 5.47 NA 3.76 NA NA 1.38 2.39 NA NA NA 2.40 3.08 1.58 51.4BDE 30 NA <1 NA NA NA NA NA <2.00 NA NA NA <0.03 no targetBDE 33 NA <1 NA NA NA NA NA 2.39 NA NA NA other no targetBDE 47 NA 23.5 NA 28.9 NA NA 14.4 24.5 NA NA NA 25.2 23.3 5.4 23.1BDE 49 NA <1 NA 8.99 NA NA NA 6.11 NA NA NA 5.15 6.75 2.00 29.6BDE 66 NA <1 NA 1.54 NA NA 0.566 0.895 NA NA NA 0.941 0.984 0.403 41.0BDE 71 NA 3.23 NA NA NA NA NA <2.00 NA NA NA NA no targetBDE 75 NA <1 NA NA NA NA NA <2.00 NA NA NA 0.102 no targetBDE 85 NA <1 NA <2.40 NA NA 0.333 0.473 NA NA NA 0.447 0.418 0.074 17.8BDE 99 NA inf NA 16.2 NA NA 8.377 11.0 NA NA NA 10.230 11.5 3.4 29.3BDE 100 NA 7.26 NA 9.55 NA NA 4.128 6.55 NA NA NA 6.749 6.85 1.93 28.3BDE 116 NA <1 NA NA NA NA NA <2.00 NA NA NA <0.04 no targetBDE 118 NA <1 NA NA NA NA NA <2.00 NA NA NA NA no targetBDE 119 NA <1 NA NA NA NA NA <2.00 NA NA NA 0.123 no targetBDE 138 NA <1 NA NA NA NA 0.049 <2.00 NA NA NA 0.101 no targetBDE 153 NA 0.382 NA <2.39 NA NA 0.601 0.5705 NA NA NA 0.508 0.515 0.097 18.8BDE 154 NA 0.399 NA <2.41 NA NA 0.621 0.610 NA NA NA 0.570 0.550 0.103 18.7BDE 155 NA 1.37 NA NA NA NA NA <2.00 NA NA NA 0.232 no targetBDE 156 NA <1 NA NA NA NA NA NA NA NA NA NA no targetBDE 181 NA <10 NA NA NA NA NA <2.00 NA NA NA <0.024 no targetBDE 183 NA <10 NA <2.42 NA NA NA <0.200 NA NA NA 0.062 no targetBDE 190 NA <10 NA NA NA NA NA <2.00 NA NA NA <0.035 no targetBDE 191 NA NA NA NA NA NA NA NA NA NA NA NA no targetBDE 196 NA NA NA NA NA NA NA NA NA NA NA NA no targetBDE 197 NA NA NA NA NA NA NA NA NA NA NA NA no targetBDE 203 NA NA NA NA NA NA NA <2.00 NA NA NA 0.065 no targetBDE 205 NA NA NA NA NA NA NA <2.00 NA NA NA NA no targetBDE 206 NA NA NA NA NA NA NA <2.00 NA NA NA <0.3 no targetBDE 207 NA NA NA NA NA NA NA <2.00 NA NA NA <0.5 no targetBDE 208 NA NA NA NA NA NA NA <2.00 NA NA NA <0.4 no targetBDE 209 NA NA NA NA NA NA NA <100 NA NA NA <5 no target

Note: Bolded values were not used in the calculation of the exercise assigned value; NA = not analyzed
6 

Table 6. SRM 2977: Laboratory means of three replicates and target values - TEO and PAHs  (reported as if three figures were significant)

Laboratory No. 1a 1c 3 4 5 6 7 8 9 10 11 12

TEO (percent) NA 6.30 NA NA 7.23 40.40 3.47 2.78 7.50 NA 1.31 NAPAHs (ng/g dry mass) Certificate Values

Laboratory No. 1a 1c 3 4 5 6 7 8 9 10 11 12 conc. 95%CL type

naphthalene 22.2 20.1 11.8 9.23 15.1 44.2 7.68 25.5 9.90 112 NA NA 19 5 Reference2-methylnaphthalene 16.3 17.6 6.43 11.5 11.0 14.2 8.59 17.4 6.76 NA NA NA 18 5 Reference1-methylnaphthalene 16.0 14.9 7.82 7.05 <8 9.15 4.23 NA 5.81 NA NA NA 16 5 Referencebiphenyl 6.81 4.48 3.39 3.81 <7 0.630 <0.2 NA 3.27 163 NA NA 6.8 0.6 Reference2,6-dimethylnaphthalene coelution 17.6 11.1 17.8 14.6 17.1 12.8 NA 14.3 NA NA 18.4 no target Targetacenaphthylene <2 <5 1.58 <0.765 <11 4.44 1.38 <6.25 3.00 3.39 NA 2.06 no target Targetacenaphthene 4.06 6.30 6.65 3.06 <8 <0.78 4.29 10.1 11.8 181 NA 3.76 4.2 0.4 Reference1,6,7-trimethylnaphthalene coelution 43.5 NA 33.1 NA 32.0 NA NA 26.5 NA NA 81.6 no target Targetfluorene 10.2 22.7 7.02 10.5 8.43 8.37 7.15 16.9 7.86 18.4 NA 10.1 10.24 0.43 Certified

phenanthrene 34.4 39.1 29.6 44.0 31.6 33.6 34.9 70.8 26.3 59.1 NA 39.5 35.1 3.8 Certified

anthracene 6.18 9.91 3.46 1.79 <10 9.14 2.32 7.77 4.74 26.7 NA 5.18 8 4 Reference1-methylphenanthrene 39.8 37.4 27.7 57.8 42.1 33.2 31.6 NA 34.0 39.9 NA 47.9 44 2 Referencefluoranthene 38.7 39.3 25.2 46.3 30.3 35.4 38.0 70.2 27.2 95.3 NA 38.2 38.7 1.0 Certified

pyrene 78.7 75.3 52.5 95.8 57.5 82.6 59.5 143 53.5 170 NA 77.4 78.9 3.5 Certified

benz[a]anthracene 21.0 19.2 14.3 25.4 17.8 16.1 17.1 38.1 13.0 NA NA 22.7 20.34 0.78 Certifiedchrysene coelution 38.9 58.7 86.6 67.0 78.6 41.4 172 54.2 coelution NA 95.6 49 2 Referencetriphenylene coelution 35.1 NA NA NA NA NA NA NA coelution NA other 39 1 Reference

benzo[b ]fluoranthene 11.4 coelution 37.4 14.1 14.1 10.9 14.7 39.4 10.2 88.7 NA 13.2 11.01 0.28 Certifiedbenzo[j]fluoranthene 4.53 coelution NA NA NA NA NA NA 8.33 NA NA other 4.6 0.2 Referencebenzo[k ]fluoranthene 4.77 3.32 3.36 12.4 7.30 10.0 4.49 24.7 NA 27.4 NA 9.39 4 1 Reference

benzo[e]pyrene 13.0 13.9 12.2 18.5 15.0 16.0 13.4 <6.25 14.3 61.6 NA 19.5 13.1 1.1 Certified

benzo[a]pyrene 8.71 10.8 5.04 6.61 7.13 6.06 5.01 <6.25 4.83 37.9 NA 10.5 8.35 0.72 Certified

perylene 3.56 4.02 <12.7 3.01 <5 3.47 NA NA 2.02 44.0 NA NA 3.50 0.76 Certified

indeno[1,2,3-cd]pyrene 4.83 coelution 3.34 4.38 8.90 4.44 4.73 <6.25 4.22 7.01 NA 5.27 4.84 0.81 Certified

dibenz[a,h]anthracene coelution coelution 1.15 1.93 <11 4.45 <0.2 <6.25 1.95 3.28 NA 1.84 1.41 0.19 Certified

benzo[ghi]perylene 9.68 9.14 5.44 10.9 <15 9.67 12.1 25.1 9.59 5.99 NA 10.1 9.53 0.43 Certified
6 

Table 7. SRM 2977: Laboratory means of three replicates and target values - Pesticides  (reported as if three figures were significant)ng/g dry mass Certificate Values

Laboratory No. 1a 1c 3 4 5 6 7 8 9 10 11 12 conc. 95%CL type

alpha-HCH (a-BHC) <2 <1 NA <0.719 <2.0 <1.8 <1 NA <0.565 0.203 NA SRM 2978 no target Target

hexachlorobenzene <2 <1 1.72 <0.684 <2.5 <1.8 <1 NA <0.355 NA NA SRM 2978 no target Targetgamma-HCH (g-BHC,lindane) <2 <1 <2.49 1.68 <1.5 <1.8 <1 NA <0.355 0.381 NA SRM 2978 no target Targetbeta-HCH (b-BHC) <2 <1 NA 7.97 NA 9.94 <1 NA <0.301 7.22 NA SRM 2978 no target Targetheptachlor <2 <5 <2.49 <0.687 <2.0 <1.8 <1 NA <0.419 1.10 NA SRM 2978 no target Targetaldrin <2 <1 <2.49 <0.689 <1.5 <1.8 <1.5 NA <0.428 0.872 NA SRM 2978 no target Targetheptachlor epoxide <2 <1 <2.49 <0.687 <2.0 <1.8 <2 NA <0.465 0.168 NA SRM 2978 no target Targetoxychlordane <2 <1 <2.49 4.90 NA <1.8 <2 NA <0.52 0.789 NA SRM 2978 no target Targetgamma-chlordane 2.20 <1 1.32 1.54 2.29 <1.8 <0.5 NA <0.427 2.88 NA SRM 2978 no target Target2,4'-DDE <2 <1 <2.49 0.723 <1.0 <1.8 <0.2 NA <1.3 1.04 NA SRM 2978 no target Targetendosulfan I <2 <1 <2.49 <1.81 <1.5 2.45 <2 NA <1.38 0.121 NA SRM 2978 no target Targetcis-chlordane (alpha-chlordane 1.34 <2 0.648 0.673 1.10 <1.8 1.34 NA <0.4 0.313 NA SRM 2978 1.42 0.13 Certified

trans-nonachlor 1.23 <2 0.563 <0.690 <1.5 2.89 1.07 NA 0.744 0.365 NA SRM 2978 1.43 0.10 Certifieddieldrin <5 <5 <2.49 5.82 4.87 8.11 5.39 NA 4.20 2.05 NA SRM 2978 6.04 0.52 Certified4,4'-DDE 12.2 12.1 6.71 10.7 4.97 9.17 10.8 NA 6.73 6.66 NA SRM 2978 12.5 1.6 Certified2,4'-DDD 3.18 4.30 2.47 Interference <4.0 3.70 2.94 NA 2.48 coelution NA SRM 2978 3.32 0.29 Certifiedendrin <2 <1 <2.49 NA <2.0 <1.8 <1.5 NA <0.512 w/ 2,4'-DDD NA SRM 2978 no target Target

endosulfan II <2 <1 <2.49 NA <3.4 <1.8 <2 NA <0.4 0.163 NA SRM 2978 no target Target4,4'-DDD 4.22 11.8 1.25 Interference 2.43 3.25 4.56 NA 2.61 2.53 NA SRM 2978 4.30 0.38 Certified

2,4'-DDT <2 <1 <2.49 <0.681 <3.0 <1.8 <1 NA <0.558 13.8 NA SRM 2978 no target Target

cis-nonachlor 0.422 <2 <2.49 <0.703 NA <1.8 <0.8 NA <0.362 1.04 NA SRM 2978 no target Target4,4'-DDT <2 <1 <2.49 <0.683 <2.5 3.74 <0.7 NA 1.18 13.8 NA SRM 2978 1.28 0.18 Certified

mirex <2 <1 <2.49 2.08 <1.5 <1.8 <0.5 NA <0.316 NA NA SRM 2978 no target Target

endosulfan sulfate <2 <1 NA NA NA <1.8 <1.5 NA <0.79 NA NA SRM 2978 no target Targetchlorpyrifos <2 NA NA NA NA <1.8 NA NA <0.408 NA NA SRM 2978 no target TargetNA = not analyzed
6 

Table 8. SRM 2977: Laboratory means of three replicates and target values - PCBs (reported as if three figures were significant)ng/g dry mass Certificate Values

Laboratory No. 1a 1c 3 4 5 6 7 8 9 10 11 12 conc. 95%CL type

PCB 8 2.05 2.05 0.961 NA <2.8 1.33 0.844 1.83 3.38 3.29 0.536 0.973 2.10 0.15 Certified

PCB 18 2.74 2.43 1.16 2.64 <2.7 5.16 1.70 1.25 2.30 1.35 0.731 1.21 2.65 0.30 CertifiedPCB 28 5.28 5.53 4.75 6.82 4.00 5.60 4.24 6.76 4.85 coelution 4.36 6.30 5.37 0.44 CertifiedPCB 31 4.05 3.77 NA 4.30 NA 3.61 3.30 3.97 NA w/ PCB 28 1.83 3.39 3.92 0.24 Certified

PCB 44 3.26 3.15 1.95 2.44 <2.4 2.70 3.35 2.05 1.37 1.68 3.17 4.57 3.25 0.63 CertifiedPCB 49 2.59 1.16 NA 2.13 NA 2.90 1.97 2.62 <0.371 3.01 0.889 1.41 no target TargetPCB 52 8.33 8.67 4.98 8.86 4.50 8.10 7.79 8.20 7.27 3.76 4.60 6.99 8.37 0.54 CertifiedPCB 66 3.57 3.44 5.87 4.15 2.65 4.86 3.51 3.79 3.64 4.26 2.32 3.73 3.64 0.32 CertifiedPCB 95 5.61 6.01 NA 6.01 NA 4.03 4.58 6.69 NA 1.87 3.81 4.53 5.39 0.59 CertifiedPCB 99 1.66 3.09 NA 4.81 NA 4.89 1.53 6.47 NA 1.73 4.66 4.18 no target TargetPCB 101 11.7 11.3 7.09 10.2 6.27 11.8 9.46 9.46 <0.4 3.50 8.60 8.77 11.2 1.2 CertifiedPCB 105 3.42 3.33 2.19 4.68 2.53 3.73 3.03 4.10 2.37 1.57 2.78 3.17 3.76 0.49 CertifiedPCB 118 10.8 9.04 13.7 12.7 6.27 11.3 10.3 11.1 9.97 4.34 8.08 9.57 10.5 1.0 CertifiedPCB 128 2.60 2.37 2.06 2.45 <1.9 3.52 2.38 2.11 1.60 0.755 1.35 1.63 2.49 0.28 CertifiedPCB 138 coelution 7.50 8.40 coelution 7.83 14.3 9.42 coelution 15.5 8.98 coelution coelution no target TargetPCB 149 10.2 9.49 NA 8.11 NA 6.48 8.22 10.0 NA 3.71 5.17 6.47 9.23 0.12 CertifiedPCB 153 coelution 15.6 7.40 18.1 6.83 12.8 13.4 10.4 9.71 11.5 10.0 11.5 14.1 1.0 CertifiedPCB 156 0.972 0.945 NA 0.772 NA 0.967 0.956 0.591 NA NA 0.597 0.847 0.960 0.085 Certified

PCB 170 3.03 2.82 1.99 2.87 <1.8 3.04 2.58 2.44 2.13 3.34 2.56 2.40 2.95 0.23 CertifiedPCB 180 6.74 7.26 3.62 6.83 3.27 5.46 4.49 5.41 8.10 10.2 coelution coelution 6.79 0.67 CertifiedPCB 187 4.59 4.13 2.63 4.41 2.60 4.89 4.75 4.63 3.22 3.06 4.61 4.05 4.76 0.38 CertifiedPCB 194 <2 0.889 NA 0.857 NA 0.990 <0.8 0.884 NA 0.604 0.361 0.680 0.897 0.042 CertifiedPCB 195 <2 <1 <2.49 <0.696 <1.8 <0.5 <0.8 <200 <0.265 0.362 0.096 0.159 no target TargetPCB 206 <2 <1 <2.49 <0.699 <1.7 <0.5 <1 <200 <0.256 0.095 0.033 0.044 no target TargetPCB 209 <2 <1 <2.49 <0.694 <1.7 <0.5 <1 <200 <0.419 0.033 0.005 <0.010 no target TargetNA = not analyzed
6 

Table 9. SRM 2977: Laboratory means of three replicates and target values - PBDEs (reported as if three figures were significant)ng/g dry mass Target Value

Laboratory No. 1a 1c 3 4 5 6 7 8 9 10 11 12 conc. std dev

BDE 15 NA <1 NA NA NA NA NA <1.00 NA NA NA 0.121 no targetBDE 17 NA 0.876 NA NA NA NA NA 1.89 NA NA NA 1.26 no targetBDE 25 NA <1 NA NA NA NA NA 1.89 NA NA NA other no targetBDE 28 NA 5.58 NA 4.42 NA NA 1.42 2.78 NA NA NA 2.79 no targetBDE 30 NA <1 NA NA NA NA NA <1.00 NA NA NA <0.024 no targetBDE 33 NA <1 NA NA NA NA NA 2.78 NA NA NA other no targetBDE 47 NA 28.1 NA 47.8 NA NA 18.9 38.2 NA NA NA 37.9 no targetBDE 49 NA <1 NA 1.95 NA NA NA 1.25 NA NA NA 1.05 1.02 0.03BDE 66 NA <1 NA <1.65 NA NA 0.310 0.552 NA NA NA 0.564 0.375 0.062BDE 71 NA 0.780 NA NA NA NA NA <1.00 NA NA NA 0.135 no targetBDE 75 NA <1 NA NA NA NA NA <1.00 NA NA NA 0.092 0.166 0.013BDE 85 NA <1 NA <1.65 NA NA 0.075 0.473 NA NA NA 0.057 no targetBDE 99 NA inf NA 7.90 NA NA 3.33 5.10 NA NA NA 4.67 4.11 0.4BDE 100 NA 5.30 NA 3.38 NA NA 1.33 2.19 NA NA NA 2.15 1.06 0.18BDE 116 NA <1 NA NA NA NA NA <1.00 NA NA NA < 0.072 no targetBDE 118 NA <1 NA NA NA NA NA <1.00 NA NA NA NA no targetBDE 119 NA <1 NA NA NA NA NA <1.00 NA NA NA 0.054 no targetBDE 138 NA <1 NA NA NA NA 0.030 <1.00 NA NA NA 0.059 no targetBDE 153 NA <LOD NA <1.64 NA NA 0.181 0.143 NA NA NA 0.147 no targetBDE 154 NA <LOD NA <1.66 NA NA 0.161 0.165 NA NA NA 0.163 no targetBDE 155 NA <LOD NA NA NA NA NA <1.00 NA NA NA 0.032 no targetBDE 156 NA <1 NA NA NA NA NA NA NA NA NA NA no targetBDE 181 NA <10 NA NA NA NA NA <1.00 NA NA NA 0.026 no targetBDE 183 NA <10 NA <1.67 NA NA NA <0.100 NA NA NA 0.057 no targetBDE 190 NA <10 NA NA NA NA NA <1.00 NA NA NA 0.028 no targetBDE 191 NA NA NA NA NA NA NA NA NA NA NA NA no targetBDE 196 NA NA NA NA NA NA NA NA NA NA NA NA no targetBDE 197 NA NA NA NA NA NA NA NA NA NA NA NA no targetBDE 203 NA NA NA NA NA NA NA <1.00 NA NA NA 0.036 no targetBDE 205 NA NA NA NA NA NA NA <1.00 NA NA NA NA no targetBDE 206 NA NA NA NA NA NA NA <1.00 NA NA NA <0.3 no targetBDE 207 NA NA NA NA NA NA NA 1.43 NA NA NA <0.5 no targetBDE 208 NA NA NA NA NA NA NA <1.00 NA NA NA <0.4 no targetBDE 209 NA NA NA NA NA NA NA <50.0 NA NA NA <5 no targetNA = not analyzed
6 

Table 10. Marine Sediment XIII (QA05SED13): Laboratory means of three replicates and exercise assigned values - Water, TOC, and PAHs  (reported as if three figures were significant)

Laboratory No. 1a 1c 2 3 4 6 7 8 10 11 12 Value s %RSD

Water (percent) 45.8 46.8 49.6 46.2 46.5 NA 52.1 53.3 46.0 44.8 43.1 47.44 3.23 6.8TOC (percent) NA NA NA 2.83 NA 1.84 NA 3.07 3.04 NA NA 2.70 0.58 21.5PAHs (ng/g dry mass) Exercise Assigned

Laboratory No. 1a 1c 2 3 4 6 7 8 10 11 12 Value s %RSD

naphthalene 864 604 614 174 928 557 994 1253 261 NA 937 785 186 23.7

2-methylnaphthalene 244 136 157 36.3 323 174 255 438 NA NA 244 219 66 30.1

1-methylnaphthalene 106 76.8 80.2 65.2 126 92.2 106 NA NA NA 133 98.2 24.0 24.4biphenyl 77.1 45.1 33.1 24.0 84.2 46.0 83.6 NA 27.6 NA 91.5 65.8 23.6 35.8

2,6-dimethylnaphthalene coelution 79.0 22.0 22.3 131 91.9 114 NA NA NA 109 81.3 43.6 53.7acenaphthylene 55.6 73.2 22.6 28.8 47.7 40.1 57.3 79.1 61.3 NA 47.0 45.1 13.8 30.6acenaphthene 36.6 inf 18.7 16.0 38.5 24.6 29.7 47.7 35.3 NA 32.1 28.9 8.4 29.01,6,7-trimethylnaphthalene coelution 19.7 NA NA 37.3 51.2 NA NA NA NA coelution no targetfluorene 66.0 52.0 46.3 29.0 67.4 52.3 56.6 114 83.4 NA 51.4 56.1 12.2 21.7

phenanthrene 332 289 178 209 452 248 425 509 298 NA 326 306 89 29.1

anthracene 167 99.4 78.4 78.7 204 98.0 178 212 189 NA 140 137 47 34.71-methylphenanthrene 58.0 51.1 28.7 37.2 85.6 79.2 54.0 NA 43.4 NA 54.7 55.4 18.2 32.8

fluoranthene 559 455 273 370 714 389 609 669 605 NA 489 496 140 28.2

pyrene 479 391 225 301 601 259 513 588 601 NA 424 421 142 33.6

benz[a ]anthracene 277 235 126 207 371 155 308 345 NA NA 247 241 80 33.2

chrysene coelution 189 134 248 coelution 225 299 498 coelution NA coelution 219 62 28.4

triphenylene coelution 84.0 NA NA NA NA NA NA w/ chrysene NA other no target

benzo[b ]fluoranthene 441 coelution 197 511 489 210 661 579 630 NA coelution 413 174 42.1benzo[j ]fluoranthene 216 coelution NA NA NA NA NA NA NA NA coelution no targetbenzo[k ]fluoranthene 228 130 91 144 coelution 203 228 443 167 NA 250 180 56 31.1benzo[e ]pyrene 326 234 121 235 353 181 341 327 399 NA 388 286 97 34.0benzo[a ]pyrene 362 451 113 196 317 144 351 344 441 NA 245 282 120 42.7

perylene 354 327 111 171 486 219 NA NA 384 NA 314 311 131 42.2

indeno[1,2,3-cd ]pyrene 332 coelution 94.6 231 372 133 340 331 316 NA 243 258 101 39.3

dibenz[a,h ]anthracene coelution coelution 11.1 49.0 coelution 47.8 59.7 97.2 96.7 NA NA 41.9 21.2 50.6

benzo[ghi ]perylene 316 196 73.5 136 321 154 328 301 329 NA 234 244 96 39.5

Note: Bolded values were not used in the calculation of the exercise assigned values; NA = not analyzed
6 

Table 11. Marine Sediment XIII (QA05SED13): Laboratory means of three replicates and exercise assigned values - Pesticides

 (reported as if three figures were significant)ng/g dry mass Exercise Assigned

Laboratory No. 1a 1c 2 3 4 6 7 8 10 11 12 Value s %RSD

alpha-HCH (a-BHC) <3 <1 NA NA <0.571 <0.15 <1 NA 0.555 NA 0.031 no targethexachlorobenzene 5.16 4.86 NA 4.12 7.01 2.40 7.48 NA NA NA 6.11 5.38 1.90 35.4

gamma-HCH (g-BHC,lindane) <3 <1 NA <0.736 16.4 <0.15 <1 NA 0.775 NA 0.030 no target

beta-HCH (b-BHC) <3 <1 NA NA <0.566 <0.15 <1 NA 0.911 NA 0.019 no targetheptachlor <3 <1 NA <0.736 <0.545 <0.15 <1 NA 4.26 NA <0.006 no targetaldrin <3 <1 NA <0.736 <0.547 <0.15 <1.5 NA 0.247 NA 0.013 no targetheptachlor epoxide <3 <1 NA <0.736 <0.545 <0.15 <2 NA 0.671 NA 0.036 no targetoxychlordane <3 <1 NA <0.736 3.99 <0.15 <2 NA 0.137 NA <0.01 no targetgamma-chlordane 0.589 <1 NA 0.658 0.583 0.341 <0.5 NA 0.775 NA 0.486 0.572 0.148 25.92,4'-DDE 0.328 <2 NA <0.736 0.499 <0.15 <0.2 NA 1.70 NA 0.314 0.380 0.103 27.1

endosulfan I <3 <1 NA <0.736 <0.548 <0.15 <2 NA 0.088 NA <0.032 no targetcis-chlordane (alpha-chlordane 0.542 <1 NA 0.451 0.54833 0.441 <0.25 NA 2.20 NA 0.430 0.482 0.058 12.0

trans-nonachlor 0.326 <1 NA 0.198 <0.548 0.383 0.19677 NA 0.382 NA 0.228 0.286 0.089 31.1dieldrin <3 <1 NA <0.736 <0.544 0.424 <1 NA 0.360 NA 0.375 0.386 0.034 8.74,4'-DDE 3.38 3.61 NA 3.85 6.57 1.46 4.11 NA 4.88 NA 2.82 3.44 1.08 31.4

2,4'-DDD <3 0.954 NA <0.736 1.80 0.484 <0.5 NA coelution NA 0.471 0.927 0.624 67.2endrin <3 <1 NA <0.736 NA <0.15 <1.5 NA coelution NA <0.016 no targetendosulfan II <3 <1 NA <0.736 NA <0.15 <2 NA 0.162 NA <0.059 no target4,4'-DDD 4.46 4.07 NA 4.88 NA 1.86 14.8 NA 2.76 NA 5.64 4.18 1.42 34.0

2,4'-DDT <3 <2 NA <0.736 0.512 <0.15 <1 NA 3.10 NA 0.097 no targetcis-nonachlor <3 <1 NA 0.172 <0.558 <0.15 3.86 NA 1.02 NA 0.167 0.454 0.493 109

4,4'-DDT <3 <2 NA <0.736 0.504 0.725 <0.7 NA 5.95 NA 0.381 0.537 0.174 32.5

mirex <3 <2 NA <0.736 4.63 1.28 <0.7 NA NA NA 0.015 no target

endosulfan sulfate <3 <1 NA NA NA <0.15 <0.5 NA NA NA <0.018 no targetchlorpyrifos <3 NA NA NA NA <0.15 <1.5 NA NA NA NA no targetNote: Bolded values were not used in the calculation of the exercise assigned values; NA = not analyzed
6 

Table 12. Marine Sediment XIII (QA05SED13): Laboratory means of three replicates and exercise assigned values - PCBs

 (reported as if three figures were significant)ng/g dry mass Exercise Assigned

Laboratory No. 1a 1c 2 3 4 6 7 8 10 11 12 Value s %RSD

PCB 8 1.48 1.76 NA 1.18 NA 0.820 1.90 3.52 7.91 0.874 1.53 1.36 0.42 30.7PCB 18 2.22 2.43 NA 1.49 2.55 1.25 2.46 1.99 2.40 0.447 1.61 2.04 0.48 23.6

PCB 28 3.76 4.71 NA 3.24 4.69 2.24 4.61 6.07 coelution 2.69 4.37 3.79 0.97 25.6PCB 31 3.34 3.65 NA NA 3.43 1.69 3.35 4.48 coelution 1.28 3.07 2.83 0.94 33.2PCB 44 3.65 3.85 NA 2.70 4.46 2.06 3.95 4.88 4.50 1.93 4.17 3.47 0.99 28.6PCB 49 4.00 4.51 NA NA 4.63 2.45 4.65 6.81 4.38 1.35 3.16 3.64 1.22 33.4PCB 52 5.38 5.45 NA 3.75 6.16 2.95 5.88 6.70 4.15 2.04 4.59 4.48 1.39 31.1PCB 66 4.97 5.42 NA 4.01 5.50 2.96 4.96 6.11 9.44 1.55 5.20 4.32 1.41 32.5PCB 95 3.98 3.70 NA NA 5.36 1.76 4.40 5.52 2.91 1.73 3.67 3.44 1.25 36.5PCB 99 2.91 3.50 NA NA 3.22 1.55 3.11 4.08 1.76 1.15 2.40 2.45 0.87 35.6PCB 101 5.15 6.30 NA 4.93 5.98 3.83 6.11 5.23 3.25 1.97 4.29 4.86 1.36 28.0PCB 105 1.48 1.53 NA 1.04 1.92 0.893 1.50 1.77 1.17 0.578 1.21 1.26 0.40 31.7PCB 118 4.19 4.21 NA 3.33 5.57 2.48 4.76 4.89 2.30 1.69 3.77 3.59 1.26 35.0PCB 128 0.663 0.662 NA 0.787 0.914 0.660 1.25 0.743 0.389 0.256 0.516 0.684 0.275 40.3PCB 138 coelution 4.77 NA 3.69 coelution 2.86 3.55 coelution 5.86 2.08 3.96 3.83 1.23 32.2PCB 149 4.39 5.54 NA NA 5.55 2.37 5.88 6.43 3.69 2.12 3.94 4.18 1.44 34.4PCB 153 coelution coelution NA 3.79 8.58 2.89 5.84 3.99 8.59 2.11 4.14 4.99 2.46 49.4PCB 156 0.517 0.565 NA NA 0.554 0.390 0.443 0.448 NA 0.165 0.473 0.444 0.128 28.7PCB 170 1.37 1.40 NA 1.24 1.79 0.877 1.08 1.41 3.05 0.522 1.05 1.19 0.36 30.5PCB 180 3.23 3.06 NA 2.44 4.41 1.52 3.11 3.08 5.04 1.23 2.61 2.97 1.15 38.8PCB 187 2.15 2.13 NA 1.51 2.54 3.36 2.69 1.80 2.89 1.05 1.84 2.20 0.69 31.3PCB 194 <2 1.74 NA NA 1.18 0.567 <0.8 1.18 1.31 0.230 0.863 1.01 0.50 49.7PCB 195 <2 0.350 NA 0.227 0.576 0.245 <0.8 <0.428 4.49 0.076 0.272 0.291 0.166 57.0PCB 206 2.38 2.68 NA 1.64 2.53 1.49 1.90 3.55 4.57 0.638 2.03 1.91 0.66 34.7PCB 209 4.63 5.49 NA 3.14 5.23 NA 4.35 6.50 2.21 1.19 4.11 4.02 1.47 36.5Note: Bolded values were not used in the calculation of the exercise assigned values; NA = not analyzed
6 

Table 13. Marine Sediment XIII (QA05SED13): Laboratory means of three replicates and exercise assigned values - PBDEs

 (reported as if three figures were significant)ng/g dry mass Exercise Assigned

Laboratory No. 1a 1c 2 3 4 6 7 8 10 11 12 Value s %RSD

BDE 15 NA <1 NA NA NA NA NA <2.14 NA NA 0.185 no targetBDE 17 NA <1 NA NA NA NA NA <2.14 NA NA 0.099 no targetBDE 25 NA <1 NA NA NA NA NA <2.14 NA NA other no targetBDE 28 NA <1 NA NA <2.1 NA 0.053 <0.214 NA NA 0.059 0.056 0.005 8.2BDE 30 NA <1 NA NA NA NA NA <2.14 NA NA <0.002 no targetBDE 33 NA <1 NA NA NA NA NA <2.14 NA NA other no targetBDE 47 NA 1.21 NA NA <2.1 NA 1.255 <10.7 NA NA 0.635 1.03 0.35 33.4BDE 49 NA <1 NA NA <2.1 NA NA <2.14 NA NA 0.154 no targetBDE 66 NA <1 NA NA <2.1 NA 0.044 <0.214 NA NA 0.032 0.038 0.009 22.7BDE 71 NA <1 NA NA NA NA NA <2.14 NA NA 0.014 no targetBDE 75 NA <1 NA NA NA NA NA <2.14 NA NA <0.002 no targetBDE 85 NA <1 NA NA <2.1 NA 0.066 <0.257 NA NA 0.012 no targetBDE 99 NA <1 NA NA <2.1 NA 1.352 <10.7 NA NA 0.452 no targetBDE 100 NA <1 NA NA <2.1 NA 0.294 <2.14 NA NA 0.109 no targetBDE 116 NA <1 NA NA NA NA NA <2.14 NA NA <0.014 no targetBDE 118 NA <1 NA NA NA NA NA <2.14 NA NA NA no targetBDE 119 NA <1 NA NA NA NA NA <2.14 NA NA 0.004 no targetBDE 138 NA <1 NA NA NA NA 0.034 <2.14 NA NA 0.008 no targetBDE 153 NA <1 NA NA <2.1 NA 0.177 <0.257 NA NA 0.064 no targetBDE 154 NA <1 NA NA <2.1 NA 0.137 <0.214 NA NA 0.069 no targetBDE 155 NA <1 NA NA NA NA NA <2.14 NA NA 0.010 no targetBDE 156 NA <1 NA NA NA NA NA NA NA NA NA no targetBDE 181 NA <10 NA NA NA NA NA <2.14 NA NA <0.141 no targetBDE 183 NA <10 NA NA <2.1 NA NA <0.214 NA NA <0.3 no targetBDE 190 NA <10 NA NA NA NA NA <2.14 NA NA <0.206 no targetBDE 191 NA NA NA NA NA NA NA NA NA NA NA no targetBDE 196 NA NA NA NA NA NA NA NA NA NA NA no targetBDE 197 NA NA NA NA NA NA NA NA NA NA NA no targetBDE 203 NA NA NA NA NA NA NA <2.14 NA NA 0.142 no targetBDE 205 NA NA NA NA NA NA NA <2.14 NA NA NA no targetBDE 206 NA NA NA NA NA NA NA <2.14 NA NA NA no targetBDE 207 NA NA NA NA NA NA NA <2.14 NA NA NA no targetBDE 208 NA NA NA NA NA NA NA <2.14 NA NA NA no targetBDE 209 NA NA NA NA NA NA NA NA NA NA 25.7 no targetNote: Bolded values were not used in the calculation of the exercise assigned values; NA = not analyzed
6 

Table 14. SRM 1941b: Laboratory means of three replicates and target values - Water, TOC, and PAHs  (reported as if three figures were significant)

Laboratory No. 1a 1c 2 3 4 6 7 8 10 11 12

Water (percent) 2.46 2.86 SRM 1944 NA 1.83 NA 92.70 36.20 NA NA NA

TOC (percent) NA NA SRM 1944 3.03 NA 0.94 NA 2.88 NA NA NA

PAHs (ng/g dry mass) Certificate Values

Laboratory No. 1a 1c 2 3 4 6 7 8 10 11 12 conc. 95%CL type

naphthalene 866 971 SRM 1944 226 877 941 933 1680 170 NA 939 848 95 Certified

2-methylnaphthalene 267 219 SRM 1944 40.9 314 308 244 445 NA NA 266 276 53 Reference

1-methylnaphthalene 136 111 SRM 1944 75.8 119 158 96.6 NA NA NA 138 127 14 Referencebiphenyl 75.8 72.5 SRM 1944 23.6 82.7 80.0 76.3 NA 26.2 NA 95.0 74.0 8.0 Reference2,6-dimethylnaphthalene coelution 128.2 SRM 1944 23.4 126 163 108 NA NA NA 122 75.9 4.5 Referenceacenaphthylene 54.4 117 SRM 1944 30.4 48.8 74.2 61.5 168 54.8 NA 55.9 53.3 6.4 Referenceacenaphthene 33.9 inf SRM 1944 16.0 40.7 44.7 31.3 132 31.3 NA 36.9 38.4 5.2 Reference1,6,7-trimethylnaphthalene coelution 31.0 SRM 1944 NA 37.2 83.3 NA NA NA NA coelution 25.5 5.1 Reference

fluorene 87.1 73.6 SRM 1944 28.1 71.0 91.3 66.1 225 82.1 NA 67.8 85 15 Certified

phenanthrene 427 466 SRM 1944 207 452 469 409 743 229 NA 389 406 44 Certified

anthracene 179 179 SRM 1944 75.6 208 179 184 345 157 NA 165 184 18 Certified

1-methylphenanthrene 71.7 77.4 SRM 1944 36.8 87.8 135 50.1 NA 35.3 NA 63.4 73.2 5.9 Certifiedfluoranthene 656 716 SRM 1944 392 726 747 565 1047 477 NA 615 651 50 Certified

pyrene 571 596 SRM 1944 307 607 480 493 924 508 NA 501 581 39 Certifiedbenz[a]anthracene 373 358 SRM 1944 221 370 280 296 562 NA NA 296 335 25 Certifiedchrysene coelution 278 SRM 1944 267 coelution 407 303 748 coelution NA coelution 291 31 Certifiedtriphenylene coelution 104 SRM 1944 NA NA NA NA NA w/ chrysene NA other 108 5 Certified

benzo[b ]fluoranthene 445 coelution SRM 1944 561 493 380 649 935 505 NA coelution 453 21 Certifiedbenzo[j ]fluoranthene 218 coelution SRM 1944 NA NA NA NA NA NA NA other 217 5 Referencebenzo[k ]fluoranthene 226 210 SRM 1944 168 coelution 367 233 690 123 NA 307 225 18 Certifiedbenzo[e ]pyrene 337 373 SRM 1944 256 362 328 328 547 331 NA 440 325 25 Certifiedbenzo[a ]pyrene 367 686 SRM 1944 197 303 241 329 535 276 NA 291 358 17 Certified

perylene 386 650 SRM 1944 148 453 305 NA NA 259 NA 375 397 45 Certifiedindeno[1,2,3-cd ]pyrene 348 coelution SRM 1944 259 369 222 350 562 178 NA 322 341 57 Certifieddibenz[a,h ]anthracene coelution coelution SRM 1944 53.4 coelution 80.9 57.2 113 95.7 NA NA 53 10 Certified

benzo[ghi ]perylene 312 353 SRM 1944 154 305 207 329 525 293 NA 308 307 45 Certified

NA = not analyzed
6 

Table 15. SRM 1941b: Laboratory means of three replicates and target values - Pesticides  (reported as if three figures were significant)ng/g dry mass Certificate Values

Laboratory No. 1a 1c 2 3 4 6 7 8 10 11 12 conc. 95%CL type

alpha-HCH (a-BHC) <3 <1 NA NA <0.679 <0.15 <0.5 NA 0.438 NA 0.027 no target Targethexachlorobenzene 5.94 10.3 NA 2.88 7.84 6.33 5.94 NA NA NA 8.59 5.83 0.38 Certified

gamma-HCH (g-BHC,lindane) <3 <1 NA <0.816 15.8 <0.15 <0.5 NA NA NA 0.024 no target Target

beta-HCH (b-BHC) <3 <1 NA NA <0.673 <0.15 <0.5 NA 0.323 NA 0.019 no target Targetheptachlor <3 <1 NA <0.816 <0.648 <0.15 <0.5 NA 3.70 NA <0.006 no target Targetaldrin <3 <1 NA <0.816 <0.650 <0.15 <0.8 NA 0.122 NA 0.011 no target Targetheptachlor epoxide <3 <1 NA <0.816 <0.648 <0.15 <1 NA 0.421 NA 0.045 no target Targetoxychlordane <3 <1 NA <0.816 3.85 <0.15 <1 NA 0.096 NA <0.025 no target Targetgamma-chlordane 0.688 <1 NA 0.488 0.586 0.674 0.543 NA 0.701 NA 0.514 0.566 0.093 Certified

2,4'-DDE 0.331 <2 NA <0.816 0.526 <0.15 0.368 NA 1.48 NA 0.342 0.38 0.12 Referenceendosulfan I <3 <1 NA <0.816 0.833 <0.15 <1 NA 0.061 NA <0.029 no target Targetcis-chlordane (alpha-chlordane 0.846 <1 NA 0.379 0.511 0.529 0.779 NA 1.84 NA 0.461 0.85 0.11 Certified

trans-nonachlor 0.436 <1 NA 0.191 <0.651 0.547 0.180 NA 0.291 NA 0.243 0.438 0.073 Certified

dieldrin <3 <1 NA <0.816 <0.647 <0.15 <0.5 NA 0.286 NA 0.403 no target Target4,4'-DDE 3.30 4.22 NA 3.35 5.72 2.83 2.52 NA 3.94 NA 3.16 3.22 0.28 Certified2,4'-DDD <3 1.57 NA <0.816 1.88 <0.15 <0.3 NA coelution NA 0.506 no target Targetendrin <3 <1 NA <0.816 NA <0.15 <0.8 NA w/ 2,4'-DDD NA <0.014 no target Target

endosulfan II <3 <1 NA <0.816 NA <0.15 <1 NA 0.044 NA <0.022 no target Target4,4'-DDD 4.55 4.97 NA 3.53 NA <0.15 4.58 NA 1.88 NA 4.74 4.66 0.46 Certified2,4'-DDT <3 <2 NA <0.816 0.575 <0.15 <0.5 NA 1.10 NA 0.089 no target Targetcis-nonachlor <3 0.756 NA 0.139 <0.664 <0.15 <0.3 NA 0.846 NA 0.183 0.378 0.053 Certified

4,4'-DDT <3 <2 NA <0.816 <0.645 <0.15 0.743 NA 0.990 NA 0.278 1.12 0.42 Referencemirex <3 <2 NA <0.816 4.69 <0.15 <0.3 NA NA NA <0.02 no target Targetendosulfan sulfate <3 <1 NA NA NA <0.15 <0.8 NA NA NA <0.023 no target Targetchlorpyrifos <3 NA NA NA NA <0.15 NA NA NA NA NA no target TargetNA = not analyzed
6 

Table 16. SRM 1941b: Laboratory means of three replicates and target values - PCBs (reported as if three figures were significant)ng/g dry mass Certificate Values

Laboratory No. 1a 1c 2 3 4 6 7 8 10 11 12 conc. 95%CL type

PCB 8 1.56 1.25 NA 0.982 NA 1.27 1.75 4.32 4.78 1.17 1.56 1.65 0.19 Certified

PCB 18 2.31 1.90 NA 1.25 2.47 1.96 2.13 2.54 1.97 1.61 1.59 2.39 0.29 CertifiedPCB 28 4.50 3.16 NA 2.93 4.72 3.65 4.01 8.17 coelution 4.72 4.56 4.52 0.57 CertifiedPCB 31 3.22 2.73 NA NA 3.30 2.59 2.87 5.48 coelution 2.34 3.09 3.18 0.41 CertifiedPCB 44 3.69 3.10 NA 2.48 4.28 3.24 3.33 5.89 3.84 5.15 5.61 3.85 0.20 CertifiedPCB 49 4.38 3.72 NA NA 4.45 3.94 4.44 8.23 3.94 2.87 3.47 4.34 0.28 CertifiedPCB 52 5.40 4.92 NA 3.24 5.68 4.48 4.98 7.78 3.60 4.34 4.86 5.24 0.28 CertifiedPCB 66 4.97 4.35 NA 3.65 5.40 4.78 4.44 7.29 7.80 4.00 6.04 4.96 0.53 CertifiedPCB 95 3.99 3.14 NA NA 4.81 2.61 3.67 6.62 2.37 4.23 3.91 3.93 0.62 CertifiedPCB 99 3.05 2.80 NA NA 3.03 2.43 2.65 5.15 1.52 3.73 2.74 2.90 0.36 CertifiedPCB 101 5.21 5.01 NA 4.19 5.43 5.87 5.30 6.46 2.69 5.91 5.29 5.11 0.34 CertifiedPCB 105 1.42 1.17 NA 0.950 1.80 1.34 1.36 2.34 0.957 1.35 1.33 1.43 0.10 CertifiedPCB 118 4.10 3.19 NA 2.90 5.17 3.84 3.77 6.33 2.20 3.94 4.08 4.23 0.19 CertifiedPCB 128 0.669 0.452 NA 0.749 0.811 1.05 0.697 0.986 0.403 0.528 0.595 0.696 0.044 Certified

PCB 138 coelution 4.24 NA 3.26 coelution 4.47 3.41 coelution 4.99 4.74 4.36 3.6 0.28 CertifiedPCB 149 4.42 4.22 NA NA 4.90 3.59 5.25 8.37 3.32 4.38 4.30 4.35 0.26 CertifiedPCB 153 coelution coelution NA 3.37 7.49 4.40 5.00 5.50 7.02 5.03 4.37 5.47 0.32 CertifiedPCB 156 0.520 <LOD NA NA 0.485 0.533 0.402 0.575 NA 0.394 0.528 0.507 0.090 Certified

PCB 170 1.42 1.01 NA 1.07 1.44 1.41 1.44 1.81 2.76 1.33 1.24 1.35 0.09 CertifiedPCB 180 3.37 2.62 NA 2.13 3.38 2.39 2.98 4.02 4.43 3.21 2.98 3.24 0.51 CertifiedPCB 187 2.17 1.55 NA 1.34 2.07 2.04 2.09 3.20 2.16 2.58 2.04 2.17 0.22 CertifiedPCB 194 <2 1.10 NA NA 0.858 0.887 0.895 1.57 1.14 0.669 1.07 1.04 0.06 CertifiedPCB 195 <2 <LOD NA 0.207 <0.657 <.05 0.409 <0.627 3.83 0.213 0.348 0.645 0.060 Certified

PCB 206 2.55 1.83 NA 1.56 2.44 <.05 2.12 4.79 3.79 2.27 2.47 2.42 0.19 CertifiedPCB 209 4.80 3.44 NA 3.08 4.95 NA 4.29 9.06 1.90 4.72 5.27 4.86 0.45 CertifiedNA = not analyzed
6 

Table 17. SRM 1941b: Laboratory means of three replicates and target values - PBDEs (reported as if three figures were significant)ng/g dry mass Target Values

Laboratory No. 1a 1c 2 3 4 6 7 8 10 11 12 conc. std dev type

BDE 15 NA <1 NA NA NA NA NA <3.13 NA NA 0.211 no target Target

BDE 17 NA <1 NA NA NA NA NA <3.13 NA NA 0.161 no target TargetBDE 25 NA <1 NA NA NA NA NA <3.13 NA NA other no target TargetBDE 28 NA <1 NA NA <2.5 NA 0.106 <0.313 NA NA 0.170 0.18 0.07 Target

BDE 30 NA <1 NA NA NA NA NA <3.13 NA NA <0.002 no target Target

BDE 33 NA <1 NA NA NA NA NA <3.13 NA NA other w/ BDE 28 Target

BDE 47 NA 0.255 NA NA <2.5 NA 1.59 <15.7 NA NA 1.61 1.48 0.51 Target

BDE 49 NA <1 NA NA <2.5 NA NA <3.13 NA NA 0.190 no target Target

BDE 66 NA <1 NA NA <2.5 NA 0.038 <0.313 NA NA 0.045 no target TargetBDE 71 NA <1 NA NA NA NA NA <3.13 NA NA 0.017 no target TargetBDE 75 NA <1 NA NA NA NA NA <3.13 NA NA 0.003 no target TargetBDE 85 NA <1 NA NA <2.4 NA 0.070 <0.376 NA NA 0.014 no target TargetBDE 99 NA <1 NA NA <2.4 NA 1.54 <15.7 NA NA 0.575 0.62 0.19 Target

BDE 100 NA <1 NA NA <2.4 NA 0.322 <3.13 NA NA 0.146 0.15 0.06 TargetBDE 116 NA <1 NA NA NA NA NA <3.13 NA NA <0.003 no target Target

BDE 118 NA <1 NA NA NA NA NA <3.13 NA NA NA no target TargetBDE 119 NA <1 NA NA NA NA NA <3.13 NA NA 0.003 no target TargetBDE 138 NA <1 NA NA NA NA 0.028 <3.13 NA NA 0.009 no target TargetBDE 153 NA <1 NA NA <2.4 NA 0.200 <0.376 NA NA 0.079 0.09 0.04 Target

BDE 154 NA <1 NA NA <2.5 NA 0.158 <0.313 NA NA 0.080 0.09 0.02 TargetBDE 155 NA <1 NA NA NA NA NA <3.13 NA NA 0.011 no target Target

BDE 156 NA <1 NA NA NA NA NA NA NA NA NA no target TargetBDE 181 NA <10 NA NA NA NA NA <3.13 NA NA <0.055 no target TargetBDE 183 NA <10 NA NA <2.5 NA NA <0.313 NA NA <0.3 0.05 0.02 Target

BDE 190 NA <10 NA NA NA NA NA <3.13 NA NA <0.08 no target Target

BDE 191 NA NA NA NA NA NA NA NA NA NA NA no target TargetBDE 196 NA NA NA NA NA NA NA NA NA NA NA no target TargetBDE 197 NA NA NA NA NA NA NA NA NA NA NA no target TargetBDE 203 NA NA NA NA NA NA NA <3.13 NA NA 0.157 no target TargetBDE 205 NA NA NA NA NA NA NA <3.13 NA NA NA no target TargetBDE 206 NA NA NA NA NA NA NA <3.13 NA NA NA no target TargetBDE 207 NA NA NA NA NA NA NA <3.13 NA NA NA no target TargetBDE 208 NA NA NA NA NA NA NA <3.13 NA NA NA no target TargetBDE 209 NA NA NA NA NA NA NA <157 NA NA 27.6 24.1 15.0 Target

NA = not analyzed
Table 18. Mussel TissueXII (QA05TIS12): z scores (25%) by laboratory - TEO and PAHs 

(z=+1 is 25% higher than the exercise assigned value; z=-1 is 25% lower than the exercise assigned value.)

Laboratory No. 1a 1c 3 4 5 6 7 8 9 10 11 12

TEO (percent) 0.3 -2.0 11.8 -3.7 -2.2 0.5 -2.2 -2.5

PAHs 

Laboratory No. 1a 1c 3 4 5 6 7 8 9 10 11 12

naphthalene 0.0 -0.7 -0.4 -1.7 1.1 13.7 1.7 4.8 -3.0 37.9

2-methylnaphthalene -1.6 -3.0 -2.4 -2.1 3.1 2.7 0.9 -3.4

1-methylnaphthalene 0.9 -3.2 0.7 -2.0 3.6 0.5 -3.2

biphenyl 2.0 0.2 0.4 -0.2 5.2 -2.4 75.5

2,6-dimethylnaphthalene 0.8 -2.5 3.1 -1.3

acenaphthylene 5.1 -0.8 -3.5 -0.1 0.0 -1.9 -0.8 1.9

acenaphthene -0.7 -2.0 4.9 -2.8 0.7 1.0 55.4

1,6,7-trimethylnaphthalene 1.4 -0.5 0.8 -1.7

fluorene 0.3 2.1 -0.1 -1.3 0.9 3.1 -2.6 2.5 -0.3

phenanthrene 0.5 0.7 -0.5 0.8 0.0 -0.3 0.2 0.2 -1.8 1.2 0.3

anthracene -0.7 2.7 1.4 -2.0 -1.0 -2.2 -1.7 22.9 3.5

1-methylphenanthrene 1.0 1.0 -0.5 1.4 0.6 -0.7 -0.4 -1.6 -2.1 1.3

fluoranthene 1.0 0.5 -0.4 1.2 -0.1 -0.2 -0.4 0.4 -1.9 3.6 0.3

pyrene 0.5 0.4 -0.3 1.1 -0.2 0.4 -0.4 0.4 -1.9 3.4 0.4

benz[a]anthracene 1.8 0.8 -0.8 1.1 -0.1 -0.5 -0.3 0.2 -2.1 0.2

chrysene -1.0 1.1 1.9 2.6 -1.1 2.9 -0.9

triphenylene

benzo[b ]fluoranthene 0.5 0.8 0.4 0.4 -0.7 0.8 1.4 -2.1 9.6

benzo[j ]fluoranthene

benzo[k ]fluoranthene 0.2 -0.9 0.4 3.7 4.1 0.3 5.1 6.6 3.5

benzo[e]pyrene 0.4 0.6 -0.3 0.6 0.1 0.0 -0.3 -0.4 -1.8 4.9 1.0

benzo[a]pyrene 0.8 2.8 -1.4 0.0 0.9 0.3 -0.4 -2.4 75.7 -0.5

perylene 0.9 1.4 0.1 15.5 -2.3 25.9

indeno[1,2,3-cd]pyrene -0.8 -1.1 0.2 1.1 -0.8 0.1 3.0 -2.6 2.2 -0.1

dibenz[a,h]anthracene

benzo[ghi]perylene -0.2 0.2 -0.9 0.6 -0.1 -0.3 1.7 3.0 -2.1 -0.4 0.7
6 

Table 19. Mussel TissueXII (QA05TIS12): z scores (25%) by laboratory - Pesticides

(z=+1 is 25% higher than the exercise assigned value; z=-1 is 25% lower than the exercise assigned value.)

Laboratory No. 1a 1c 3 4 5 6 7 8 9 10 11 12

alpha-HCH (a-BHC)

hexachlorobenzene

gamma-HCH (g-BHC,lindane)

beta-HCH (b-BHC)

heptachlor

aldrin

heptachlor epoxide

oxychlordane

gamma-chlordane 0.5 -0.1 0.5 -0.9 0.5 -0.5 0.3 -0.6 0.3

2,4'-DDE

endosulfan I

cis-chlordane (alpha-chlordane) 1.5 1.9 -0.9 -0.4 -1.2 -0.4 0.0 -2.0 -2.2 -0.5

trans-nonachlor 0.2 0.1 -0.5 0.4 0.1 0.5 -1.2 -1.6 -1.7 0.4

dieldrin -1.9 0.3 4.4 1.4 -2.0 -3.2 -2.2

4,4'-DDE 0.8 1.5 -0.7 0.1 -1.3 -0.4 -1.0 -1.8 -0.5 -0.4

2,4'-DDD -1.2 1.4 -0.5 3.1 -0.2 0.0 -1.0 -1.2 -0.6

endrin

endosulfan II

4,4'-DDD -1.0 1.1 -0.2 3.9 -0.6 -1.3 -1.3 -1.2 1.5

2,4'-DDT

cis-nonachlor 1.0 0.3 -1.1 0.1 -0.4 -0.4 -0.5 1.1 0.0

4,4'-DDT -1.1 2.0 20.4 -1.3 6.9 0.4

mirex

endosulfan sulfate

chlorpyrifos
6 

Table 20 Mussel TissueXII (QA05TIS12): z scores (25%) by laboratory - PCBs

(z=+1 is 25% higher than the exercise assigned value; z=-1 is 25% lower than the exercise assigned value.)

Laboratory No. 1a 1c 3 4 5 6 7 8 9 10 11 12

PCB 8 -0.5 0.3 -1.3 -3.5 -0.6 1.5 -1.6 14.5 -1.2 -0.7

PCB 18 1.9 2.1 -1.0 1.5 -1.2 -3.4 -0.2 -0.9 -1.2 0.7 -0.6 -0.5

PCB 28 1.2 0.8 -0.6 0.2 -0.5 -3.5 -0.4 0.3 -1.6 0.0 0.6

PCB 31 0.1 0.8 -0.1 -3.5 -0.9 -0.1 -0.9 0.2

PCB 44 0.9 0.7 -1.2 0.3 -1.4 -3.5 -0.9 -1.0 -1.9 -1.6 1.8 2.4

PCB 49 1.3 1.6 1.1 -3.4 -1.0 0.5 -1.4 -1.7 -0.3 -0.1

PCB 52 0.9 1.7 -0.7 0.7 -0.8 -3.5 -0.4 -0.5 -1.3 -2.1 -0.1 0.4

PCB 66 0.4 0.7 -0.4 0.3 2.1 -3.4 -1.3 -0.8 -1.5 0.2 -0.5 0.7

PCB 95 0.6 1.0 0.2 -3.6 -1.1 -0.3 -2.7 -0.4 0.0

PCB 99 0.0 -0.3 0.1 -3.5 -1.2 0.2 -2.7 0.7 0.4

PCB 101 0.4 1.1 -0.2 -0.1 0.5 -3.4 -0.8 -1.0 -1.6 -2.8 0.0 0.1

PCB 105 0.5 0.7 -0.7 0.4 0.8 -3.4 -0.7 0.1 -1.3 -2.7 0.1 0.2

PCB 118 0.3 0.0 0.2 0.7 1.1 -3.4 -1.4 0.0 -1.3 -2.6 0.0 0.5

PCB 128 0.5 0.5 0.6 0.9 0.1 -3.3 -1.2 -0.6 -1.4 -2.6 -0.5 -0.3

PCB 138 -0.2 0.3 1.7 -3.3 -0.4 -0.8 -0.6

PCB 149 0.3 1.2 -0.3 -3.6 -0.9 -0.3 -2.3 -0.7 -0.1

PCB 153 2.8 -0.6 1.8 0.0 -3.5 -1.6 -1.2 -1.0 -0.6 -0.1 0.5

PCB 156 0.8 -0.3 0.4 -3.5 -1.1 -1.1 0.3 1.1

PCB 170 0.1 -0.7 -0.9 -0.2 0.9 -3.1 0.4 1.6 -0.4 -0.7

PCB 180 -0.5 -0.9 -1.0 0.4 -1.0 -3.6 0.0 -1.1 2.7 1.4

PCB 187 0.4 0.3 -0.5 0.9 0.0 -3.4 -0.6 -0.9 -1.2 -0.8 1.5 0.9

PCB 194 0.7 0.3 -0.8 0.4 -1.5 -0.7

PCB 195

PCB 206

PCB 209
6 

Table 21 Mussel TissueXII (QA05TIS12): z scores (25%) by laboratory - PBDEs

(z=+1 is 25% higher than the exercise assigned value; z=-1 is 25% lower than the exercise assigned value.)

1a 1c 3 4 5 6 7 8 9 10 11 12

BDE 15

BDE 17 -0.8 0.6 0.2

BDE 25

BDE 28 3.1 0.9 -2.2 -0.9 -0.9

BDE 30

BDE 33

BDE 47 0.0 1.0 -1.5 0.2 0.3

BDE 49 1.3 -0.4 -0.9

BDE 66 2.2 -1.7 -0.4 -0.2

BDE 71

BDE 75

BDE 85 -0.8 0.5 0.3

BDE 99 1.7 -1.1 -0.2 -0.4

BDE 100 0.2 1.6 -1.6 -0.2 -0.1

BDE 116

BDE 118

BDE 119

BDE 138

BDE 153 -1.0 0.7 0.4 -0.1

BDE 154 -1.1 0.5 0.4 0.1

BDE 155

BDE 156

BDE 181

BDE 183

BDE 190

BDE 191

BDE 196

BDE 197

BDE 203

BDE 205

BDE 206

BDE 207

BDE 208

BDE 209
6 

Table 22. Marine Sediment XIII (QA05SED13): z scores (25% by laboratory)- water, TOC, and PAHs 

(z=+1 is 25% higher than the exercise assigned value; z=-1 is 25% lower than the exercise assigned value.)

Laboratory No. 1a 1c 2 3 4 6 7 8 10 11 12

Water (percent) -0.1 -0.1 0.2 -0.1 -0.1 0.4 0.5 -0.1 -0.2 -0.4

TOC 0.2 -1.3 0.6 0.5

PAHs

Laboratory No. 1a 1c 2 3 4 6 7 8 10 11 12

naphthalene 0.4 -0.9 -0.9 -3.1 0.7 -1.2 1.1 2.4 -2.7 0.8

2-methylnaphthalene 0.5 -1.5 -1.1 -3.3 1.9 -0.8 0.7 0.5

1-methylnaphthalene 0.3 -0.9 -0.7 -1.3 1.1 -0.2 0.3 1.4

biphenyl 0.7 -1.3 -2.0 -2.5 1.1 -1.2 1.1 -2.3 1.6

2,6-dimethylnaphthalene -0.1 -2.9 -2.9 2.4 0.5 1.6 1.4

acenaphthylene 0.9 2.5 -2.0 -1.4 0.2 -0.4 1.1 3.0 1.4 0.2

acenaphthene 1.1 -1.4 -1.8 1.3 -0.6 0.1 2.6 0.9 0.4

1,6,7-trimethylnaphthalene

fluorene 0.7 -0.3 -0.7 -1.9 0.8 -0.3 0.0 4.1 2.0 -0.3

phenanthrene 0.3 -0.2 -1.7 -1.3 1.9 -0.8 1.6 2.6 -0.1 0.3

anthracene 0.9 -1.1 -1.7 -1.7 2.0 -1.1 1.2 2.2 1.5 0.1

1-methylphenanthrene 0.2 -0.3 -1.9 -1.3 2.2 1.7 -0.1 -0.9 0.0

fluoranthene 0.5 -0.3 -1.8 -1.0 1.8 -0.9 0.9 1.4 0.9 -0.1

pyrene 0.5 -0.3 -1.9 -1.1 1.7 -1.5 0.9 1.6 1.7 0.0

benz[a ]anthracene 0.6 -0.1 -1.9 -0.6 2.2 -1.4 1.1 1.7 0.1

chrysene -0.6 -1.6 0.5 0.1 1.5 5.1

triphenylene

benzo[b ]fluoranthene 0.3 -2.1 1.0 0.7 -2.0 2.4 1.6 2.1

benzo[j ]fluoranthene

benzo[k ]fluoranthene 1.1 -1.1 -2.0 -0.8 0.5 1.1 5.8 -0.3 1.6

benzo[e ]pyrene 0.6 -0.7 -2.3 -0.7 0.9 -1.5 0.8 0.6 1.6 1.4

benzo[a ]pyrene 1.1 2.4 -2.4 -1.2 0.5 -2.0 1.0 0.9 2.3 -0.5

perylene 0.5 0.2 -2.6 -1.8 2.2 -1.2 0.9 0.0

indeno[1,2,3-cd ]pyrene 1.1 -2.5 -0.4 1.8 -1.9 1.3 1.1 0.9 -0.2

dibenz[a,h ]anthracene -2.9 0.7 0.6 1.7 5.3 5.2

benzo[ghi ]perylene 1.2 -0.8 -2.8 -1.8 1.3 -1.5 1.4 0.9 1.4 -0.2
6 

Table 23. Marine Sediment XIII (QA05SED13): z scores (25% by laboratory)- pesticides

(z=+1 is 25% higher than the exercise assigned value; z=-1 is 25% lower than the exercise assigned value.)

Laboratory No. 1a 1c 2 3 4 6 7 8 10 11 12

alpha-HCH (a-BHC)

hexachlorobenzene -0.2 -0.4 -0.9 1.2 -2.2 1.6 0.5

gamma-HCH (g-BHC,lindane)

beta-HCH (b-BHC)

heptachlor

aldrin

heptachlor epoxide

oxychlordane

gamma-chlordane 0.1 0.6 0.1 -1.6 1.4 -0.6

2,4'-DDE -0.5 1.2 13.9 -0.7

endosulfan I

cis-chlordane (alpha-chlordane 0.5 -0.3 0.5 -0.3 14.2 -0.4

trans-nonachlor 0.6 -1.2 1.4 -1.2 1.3 -0.8

dieldrin 0.4 -0.3 -0.1

4,4'-DDE -0.1 0.2 0.5 3.6 -2.3 0.8 1.7 -0.7

2,4'-DDD 0.1 3.8 -1.9 -2.0

endrin

endosulfan II

4,4'-DDD 0.3 -0.1 0.7 -2.2 10.2 -1.4 1.4

2,4'-DDT

cis-nonachlor -2.5 30.0 5.0 -2.5

4,4'-DDT -0.2 1.4 40.3 -1.2

mirex

endosulfan sulfate

chlorpyrifos
6 

Table 24. Marine Sediment XIII (QA05SED13): z scores (25% by laboratory)- PCBs

(z=+1 is 25% higher than the exercise assigned value; z=-1 is 25% lower than the exercise assigned value.)

Laboratory No. 1a 1c 2 3 4 6 7 8 10 11 12

PCB 8 0.3 1.1 -0.5 -1.6 1.6 6.3 19.2 -1.4 0.5

PCB 18 0.3 0.8 -1.1 1.0 -1.6 0.8 -0.1 0.7 -3.1 -0.8

PCB 28 0.0 1.0 -0.6 0.9 -1.6 0.9 2.4 -1.2 0.6

PCB 31 0.7 1.2 0.9 -1.6 0.7 2.3 -2.2 0.3

PCB 44 0.2 0.4 -0.9 1.1 -1.6 0.5 1.6 1.2 -1.8 0.8

PCB 49 0.4 1.0 1.1 -1.3 1.1 3.5 0.8 -2.5 -0.5

PCB 52 0.8 0.9 -0.7 1.5 -1.4 1.2 2.0 -0.3 -2.2 0.1

PCB 66 0.6 1.0 -0.3 1.1 -1.3 0.6 1.7 4.7 -2.6 0.8

PCB 95 0.6 0.3 2.2 -1.9 1.1 2.4 -0.6 -2.0 0.3

PCB 99 0.8 1.7 1.3 -1.5 1.1 2.7 -1.1 -2.1 -0.1

PCB 101 0.2 1.2 0.1 0.9 -0.8 1.0 0.3 -1.3 -2.4 -0.5

PCB 105 0.7 0.8 -0.7 2.1 -1.2 0.8 1.6 -0.3 -2.2 -0.2

PCB 118 0.7 0.7 -0.3 2.2 -1.2 1.3 1.5 -1.4 -2.1 0.2

PCB 128 -0.1 -0.1 0.6 1.3 -0.1 3.3 0.3 -1.7 -2.5 -1.0

PCB 138 1.0 -0.1 -1.0 -0.3 2.1 -1.8 0.1

PCB 149 0.2 1.3 1.3 -1.7 1.6 2.2 -0.5 -2.0 -0.2

PCB 153 -1.0 2.9 -1.7 0.7 -0.8 2.9 -2.3 -0.7

PCB 156 0.7 1.1 1.0 -0.5 0.0 0.0 -2.5 0.3

PCB 170 0.6 0.7 0.2 2.0 -1.1 -0.4 0.7 6.2 -2.3 -0.5

PCB 180 0.3 0.1 -0.7 1.9 -2.0 0.2 0.1 2.8 -2.3 -0.5

PCB 187 -0.1 -0.1 -1.3 0.6 2.1 0.9 -0.7 1.3 -2.1 -0.7

PCB 194 2.9 0.7 -1.8 0.7 1.2 -3.1 -0.6

PCB 195 0.8 -0.9 3.9 -0.6 57.8 -3.0 -0.3

PCB 206 1.0 1.6 -0.6 1.3 -0.9 0.0 3.4 5.6 -2.7 0.3

PCB 209 0.6 1.5 -0.9 1.2 0.3 2.5 -1.8 -2.8 0.1
6 

Table 25. Marine Sediment XIII (QA05SED13): z scores (25% by laboratory)- PBDEs

(z=+1 is 25% higher than the exercise assigned value; z=-1 is 25% lower than the exercise assigned value.)

Laboratory No. 1a 1c 2 3 4 6 7 8 10 11 12

BDE 15

BDE 17

BDE 25

BDE 28 -0.2 0.2

BDE 30

BDE 33

BDE 47 0.7 0.9 -1.5

BDE 49

BDE 66 0.6 -0.6

BDE 71

BDE 75

BDE 85

BDE 99

BDE 100

BDE 116

BDE 118

BDE 119

BDE 138

BDE 153

BDE 154

BDE 155

BDE 156

BDE 181

BDE 183

BDE 190

BDE 191

BDE 196

BDE 197

BDE 203

BDE 205

BDE 206

BDE 207

BDE 208

BDE 209
Table 26. Mussel Tissue XII (QA05TIS12): RSDs for three replicates - TEO and PAHs

Lab 1a Lab 1c Lab 3 Lab 4 Lab 5 Lab 6

Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977

rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd

TEO or lipid 7.9% 1.9% 15.8% 4.9% 10.2% 28.6%PAH ANALYSES Lab 1a Lab 1c Lab 3 Lab 4 Lab 5 Lab 6

Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977

rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd

naphthalene 1.7% 4.3% 7.5% 9.1% 10.3% 24.9% 0.9% 2.3% 23.8% 8.4% 26.1% 17.4%2-methylnaphthalene 5.8% 2.2% 70.7% 4.5% 18.3% 8.4% 4.2% 1.3% 11.6% 2.5%1-methylnaphthalene 4.8% 3.6% 89.8% 7.2% 12.5% 9.5% 7.9% 0.6% 6.0% 3.3%biphenyl 1.3% 2.3% 7.9% 6.8% 17.2% 36.3% 5.1% 25.9% 7.6% 5.7%2,6-dimethylnaphthalene 3.6% 7.0% 24.1% 4.7% 0.6% 19.8% 52.8% 4.0%acenaphthylene 8.7% 5.4% 8.7% 0.0% 6.0% 14.3%acenaphthene 7.6% 1.0% 17.3% 2.6% 17.7% 14.5% 13.6% 4.2%1,6,7-trimethylnaphthalene 7.8% 2.5% 3.2% 5.1% 14.3% 24.7%fluorene 3.2% 4.2% 7.3% 2.2% 18.1% 3.2% 1.4% 1.1% 7.2% 10.9% 7.6%phenanthrene 6.5% 3.3% 0.5% 1.9% 6.5% 15.8% 1.4% 1.0% 10.5% 6.0% 7.7% 20.0%anthracene 4.3% 1.5% 5.4% 6.2% 55.4% 25.4% 8.6% 25.8% 18.2% 7.2%1-methylphenanthrene 3.5% 2.0% 3.4% 2.2% 2.7% 11.7% 1.4% 1.1% 3.7% 5.0% 10.7% 14.1%fluoranthene 1.1% 2.4% 0.6% 2.7% 9.2% 8.3% 2.0% 0.9% 9.2% 4.7% 8.0% 7.3%pyrene 1.7% 1.2% 0.2% 3.5% 7.1% 8.8% 2.3% 0.4% 9.2% 5.7% 12.2% 8.3%benz[a]anthracene 9.2% 3.8% 3.2% 0.7% 11.4% 4.9% 3.1% 1.9% 11.7% 5.1% 5.8% 0.9%chrysene 2.4% 1.7% 7.1% 8.1% 2.0% 0.7% 10.5% 5.5% 7.4% 10.4%triphenylene 1.4% 2.4%

benzo[b ]fluoranthene 1.7% 4.4% 8.7% 32.7% 5.7% 0.7% 12.3% 8.9% 4.6% 1.3%benzo[j]fluoranthene 6.2% 1.1%benzo[k ]fluoranthene 4.7% 2.4% 6.9% 4.3% 10.5% 16.7% 2.7% 1.2% 12.7% 3.6% 10.6% 6.0%

benzo[e]pyrene 3.2% 3.2% 1.8% 2.2% 9.3% 8.6% 2.0% 0.6% 10.0% 4.8% 6.3% 4.2%benzo[a]pyrene 1.5% 2.2% 4.4% 8.0% 18.0% 6.4% 13.3% 1.9% 17.0% 4.3% 1.7% 12.8%perylene 3.9% 3.9% 4.7% 5.6% 8.0% 2.7% 3.1% 8.8%indeno[1,2,3-cd]pyrene 3.5% 1.7% 23.0% 14.5% 6.7% 0.8% 9.8% 4.1% 24.2% 19.5%dibenz[a,h]anthracene 3.9% 38.2% 3.3% 1.5% 5.1%

benzo[ghi]perylene 4.3% 3.2% 3.6% 0.8% 8.9% 12.3% 1.1% 0.0% 10.0% 13.8% 17.9%
6 

Table 26 (cont). Mussel Tissue XII (QA05TIS12): RSDs for three replicates - TEO and PAHs

Lab 7 Lab 8 Lab 9 Lab 10 Lab 11 Lab 12

Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 SRM 2978

rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd

TEO or lipid 2.4% 6.2% 0.0% 0.0% 16.8% 7.1% 11.9% 4.4% 0.0%PAH ANALYSES Lab 7 Lab 8 Lab 9 Lab 10 Lab 11 Lab 12

Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 SRM 2978

rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd

naphthalene 38.3% 28.9% 6.5% 19.4% 16.7% 15.0% 18.2% 15.3%2-methylnaphthalene 15.8% 18.3% 14.7% 30.6% 10.0% 3.2%1-methylnaphthalene 32.6% 11.4% 7.0% 5.2%biphenyl 13.2% 4.9% 21.7% 9.0%2,6-dimethylnaphthalene 8.8% 19.1% 4.7% 5.8%acenaphthylene 54.1% 52.6% 1.3% 9.3% 17.4% 3.7% 25.2% 9.3%acenaphthene 62.4% 10.6% 0.0% 26.6% 13.1% 23.1% 10.7% 13.7%1,6,7-trimethylnaphthalene 11.6% 38.9% 7.1% 14.1%fluorene 17.3% 6.5% 45.7% 19.3% 1.5% 19.2% 2.1% 31.4% 3.7%phenanthrene 21.5% 19.8% 22.7% 58.3% 6.1% 3.6% 16.3% 12.2% 0.8% 0.5%anthracene 67.9% 12.8% 0.0% 17.6% 21.1% 10.1% 0.4% 22.7% 6.1%1-methylphenanthrene 8.2% 18.7% 4.4% 1.5% 29.0% 24.2% 1.0% 14.1%fluoranthene 9.5% 29.4% 23.2% 53.4% 6.1% 2.5% 19.9% 8.4% 2.1% 3.4%pyrene 8.9% 8.6% 22.2% 53.0% 5.1% 3.8% 19.7% 16.6% 2.0% 2.6%benz[a]anthracene 15.1% 13.6% 18.9% 55.7% 5.7% 8.1% 0.9% 4.1%chrysene 10.0% 11.4% 19.3% 63.5% 5.6% 2.2% 22.4% 6.1% 2.2% 1.5%triphenylenebenzo[b ]fluoranthene 7.6% 12.0% 33.3% 0.0% 8.2% 6.4% 9.4% 11.1% 4.9% 10.2%

benzo[j]fluoranthene 9.2% 5.3%

benzo[k ]fluoranthene 22.2% 20.0% 14.4% 71.6% 8.2% 1.6% 5.2% 6.0%

benzo[e]pyrene 9.4% 10.6% 18.0% 10.1% 4.9% 18.0% 23.2% 5.5% 7.4%benzo[a]pyrene 34.5% 20.9% 3.0% 9.2% 52.6% 3.0% 6.0% 23.9%perylene 3.1% 10.4% 24.7% 11.5%indeno[1,2,3-cd]pyrene 24.2% 33.1% 5.9% 2.6% 4.4% 38.2% 29.4% 3.6% 24.2%dibenz[a,h]anthracene 7.7% 3.9% 43.8% 26.5% 3.7% 5.9%

benzo[ghi]perylene 17.6% 17.3% 3.4% 37.7% 4.5% 6.4% 37.8% 12.7% 2.5% 2.5%
6 

Table 27. Mussel Tissue XII (QA05TIS12): RSDs for three replicates - Pesticides

Lab 1a Lab 1c Lab 3 Lab 4 Lab 5 Lab 6

Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977

rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd

alpha-HCH (a-BHC)hexachlorobenzene 44.7% 24.2%gamma-HCH (g-BHC,lindane) 3.7% 12.4%beta-HCH (b-BHC) 8.6% 0.6% 35.2% 1.9%heptachloraldrin 3.5%heptachlor epoxideoxychlordane 12.1% 8.8% 8.1%gamma-chlordane 1.3% 2.8% 3.0% 5.4% 2.0% 0.6% 6.2% 5.7% 3.9%2,4'-DDE 10.6% 6.8%endosulfan I 7.7% 7.6%cis-chlordane (alpha-chlordane) 2.5% 5.6% 2.5% 8.1% 15.3% 2.1% 10.1% 9.5% 0.0% 14.9%trans-nonachlor 2.7% 5.8% 1.0% 9.5% 20.6% 1.6% 8.9% 11.6% 9.4%dieldrin 14.3% 0.5% 13.8% 7.8% 9.2% 3.3%4,4'-DDE 2.2% 2.9% 0.6% 7.7% 7.9% 15.6% 3.0% 0.0% 2.3% 13.7% 5.1% 11.0%2,4'-DDD 5.4% 3.7% 0.5% 5.8% 13.3% 17.0% 4.9% 1.8% 18.1% 13.6%endrinendosulfan II4,4'-DDD 8.4% 3.7% 0.9% 2.5% 9.1% 31.6% 11.9% 5.7% 8.6% 13.3%2,4'-DDT 16.0%cis-nonachlor 3.5% 4.2% 3.3% 9.8% 4.3% 27.8%4,4'-DDT 11.1% 0.0% 14.7% 44.2%mirex 7.2% 6.3%endosulfan sulfatechlorpyrifos
6 

Table 27 (cont). Mussel Tissue XII (QA05TIS12): RSDs for three replicates - Pesticides

Lab 7 Lab 8 Lab 9 Lab 10 Lab 11 Lab 12

Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 SRM 2978

rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd

alpha-HCH (a-BHC) 6.3% 0.7% 13.8% 18.2%

hexachlorobenzene 7.4% 9.4%gamma-HCH (g-BHC,lindane) 1.9% 44.8% 11.5% 12.3%beta-HCH (b-BHC) 50.0% 0.6% 11.4%heptachlor 52.9% 27.8% 21.4% 18.5%aldrin 37.4% 19.3% 19.1%heptachlor epoxide 10.2% 29.1% 1.7% 6.9%oxychlordane 2.4% 26.2% 4.2% 17.8%gamma-chlordane 28.5% 22.7% 3.7% 24.0% 4.5% 1.1%2,4'-DDE 8.7% 25.8% 6.6% 5.3%endosulfan I 46.8% 40.5%cis-chlordane (alpha-chlordane) 22.7% 28.3% 3.3% 4.9% 11.3% 2.5% 0.1%trans-nonachlor 31.8% 30.3% 3.9% 4.5% 12.3% 8.3% 1.9% 1.8%dieldrin 5.1% 28.9% 8.6% 7.7% 10.3% 31.8% 3.8% 7.7%4,4'-DDE 10.5% 11.2% 2.9% 1.8% 6.7% 21.8% 4.1% 1.9%2,4'-DDD 4.7% 12.4% 8.2% 22.5% 10.8% 32.6% 17.4% 1.2%endrinendosulfan II 100.8% 88.3%4,4'-DDD 12.0% 22.9% 6.2% 19.1% 5.8% 19.5% 10.4% 3.6%2,4'-DDT 42.9% 57.9% 8.1% 10.5%cis-nonachlor 23.3% 4.8% 6.2% 15.0% 4.5% 0.3%4,4'-DDT 3.3% 10.3% 24.0% 7.7% 3.4% 1.8%mirex 4.9% 2.7%endosulfan sulfatechlorpyrifos
6 

Table 28. Mussel Tissue XII (QA05TIS12): RSDs for three replicates - PCBs

Lab 1a Lab 1c Lab 3 Lab 4 Lab 5 Lab 6

Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977

rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd

PCB 8 6.2% 5.0% 4.8% 1.8% 11.0% 7.1% 11.5% 13.2%PCB 18 3.7% 2.3% 3.3% 2.6% 9.4% 15.5% 1.2% 3.1% 2.5% 11.2% 28.4%PCB 28 1.3% 2.3% 1.4% 1.7% 10.7% 9.8% 2.6% 6.1% 5.9% 18.0% 2.5% 10.5%PCB 31 2.2% 1.8% 1.8% 1.9% 1.8% 2.0% 7.6% 5.4%PCB 44 2.9% 1.4% 0.7% 6.0% 10.9% 20.0% 1.4% 2.3% 3.1% 4.5% 8.8%PCB 49 5.8% 3.4% 6.4% 3.8% 1.4% 2.3% 5.2% 7.6%PCB 52 2.9% 0.7% 0.4% 1.3% 11.3% 6.9% 1.5% 0.7% 4.6% 19.0% 4.9% 1.7%PCB 66 4.2% 1.6% 2.5% 2.1% 10.6% 25.2% 2.0% 0.8% 5.4% 2.7% 6.1% 2.9%PCB 95 6.4% 3.3% 1.8% 5.8% 1.3% 1.8% 6.3% 6.5%PCB 99 1.8% 3.7% 1.8% 7.5% 1.5% 0.1% 4.8% 3.4%PCB 101 3.5% 5.0% 0.7% 1.8% 12.2% 5.0% 1.3% 1.1% 3.0% 17.8% 6.1% 1.7%PCB 105 7.3% 4.9% 0.2% 1.3% 13.4% 9.7% 1.8% 1.7% 3.1% 16.0% 5.1% 9.8%PCB 118 2.5% 7.2% 0.1% 2.8% 12.8% 2.6% 1.0% 2.4% 4.0% 14.8% 5.4% 3.1%PCB 128 1.0% 7.0% 0.9% 3.1% 13.0% 14.1% 0.6% 1.6% 2.6% 8.4% 5.4%PCB 138 3.3% 7.6% 9.7% 12.4% 1.1% 0.8% 3.0% 16.0% 5.2% 2.4%PCB 149 2.8% 5.8% 1.2% 6.4% 2.0% 2.0% 4.4% 4.1%PCB 153 0.3% 4.2% 9.6% 14.1% 1.2% 1.1% 2.4% 16.4% 5.6% 0.3%PCB 156 1.0% 3.9% 1.9% 8.6% 1.4% 3.7% 8.6% 12.4%PCB 170 1.3% 3.9% 4.3% 3.2% 8.6% 11.6% 7.3% 1.8% 0.0% 9.2% 3.3%PCB 180 7.4% 1.7% 1.1% 5.4% 10.6% 14.4% 1.5% 1.1% 6.6% 18.7% 1.0% 5.9%PCB 187 2.8% 2.3% 1.4% 7.2% 12.5% 14.2% 0.9% 0.0% 8.1% 0.0% 5.2% 2.5%PCB 194 14.6% 4.7% 0.0% 7.3% 32.9%PCB 195PCB 206PCB 209
6 

Table 28 (cont). Mussel Tissue XII (QA05TIS12): RSDs for three replicates - PCBs

Lab 7 Lab 8 Lab 9 Lab 10 Lab 11 Lab 12

Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 SRM 2978

rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd

PCB 8 20.7% 22.2% 3.0% 30.9% 26.5% 27.1% 10.9% 26.0% 4.5% 50.0% 9.5% 10.6%PCB 18 38.2% 18.7% 0.3% 29.1% 7.1% 19.6% 8.2% 22.9% 10.3% 62.9% 9.8% 14.2%PCB 28 31.5% 15.4% 1.7% 26.4% 2.3% 10.2% 6.2% 23.1% 9.1% 14.9% 9.6% 9.3%PCB 31 7.7% 10.9% 6.6% 23.5% 17.5% 18.5% 12.9% 12.6%PCB 44 5.7% 25.5% 3.9% 41.1% 3.6% 19.1% 6.3% 28.3% 5.2% 25.9% 9.9% 10.8%PCB 49 41.7% 5.3% 11.1% 46.4% 4.0% 6.0% 23.2% 18.5% 27.3% 9.8% 10.3%PCB 52 4.4% 16.8% 5.6% 46.7% 4.7% 3.4% 5.6% 21.8% 9.5% 29.4% 9.8% 10.1%PCB 66 28.0% 16.8% 4.9% 32.7% 6.1% 11.0% 6.1% 23.0% 13.6% 8.9% 6.9% 8.2%PCB 95 13.3% 12.8% 8.7% 50.9% 7.3% 21.8% 11.3% 21.7% 9.2% 8.4%PCB 99 21.2% 24.8% 5.3% 48.3% 6.9% 22.9% 12.8% 5.6% 10.3% 5.8%PCB 101 10.4% 9.8% 10.0% 33.5% 4.5% 6.4% 21.7% 10.8% 6.4% 6.7% 9.0%PCB 105 16.9% 11.2% 2.4% 38.6% 3.1% 5.3% 6.6% 20.6% 8.0% 3.5% 1.4% 6.8%PCB 118 8.5% 2.9% 5.0% 36.5% 4.7% 7.5% 6.8% 22.2% 5.7% 3.6% 2.4% 6.1%PCB 128 7.2% 7.6% 3.2% 38.2% 2.9% 1.3% 9.0% 23.4% 12.2% 10.8% 4.3% 4.9%PCB 138 6.9% 29.4% 3.2% 39.0% 24.6% 28.8% 7.1% 22.6% 2.9% 1.0% 6.8% 5.2%PCB 149 6.7% 17.1% 16.5% 57.4% 6.3% 22.1% 3.9% 11.1% 13.2% 4.3%PCB 153 10.0% 7.2% 4.6% 37.6% 4.6% 4.8% 6.6% 20.6% 3.3% 2.2% 4.9% 5.3%PCB 156 16.7% 20.4% 3.5% 41.3% 4.0% 2.0% 3.3% 2.4%PCB 170 11.6% 4.7% 35.1% 3.4% 8.7% 24.4% 14.6% 3.1% 4.9% 5.1%PCB 180 5.9% 19.2% 7.2% 36.6% 0.4% 15.3% 9.3% 24.2% 14.9% 1.9% 4.5% 6.5%PCB 187 5.1% 6.9% 8.6% 12.1% 5.0% 9.3% 6.5% 17.9% 15.5% 10.2% 5.2% 2.4%PCB 194 3.8% 44.3% 6.5% 28.3% 27.4% 16.4% 13.9% 6.1%PCB 195 17.6% 24.6% 10.6% 5.2% 15.8% 10.0%

PCB 206 44.3% 13.4% 15.1% 9.2% 6.5% 3.8%PCB 209 34.8% 51.4% 8.1% 65.5% 10.0%
6 

Table 29. Mussel Tissue XII (QA05TIS12): RSDs for three replicates - PBDEs

Lab 1a Lab 1c Lab 3 Lab 4 Lab 5 Lab 6

Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977

rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd

BDE 15BDE 17 1.4% 14.9%BDE 25BDE 28 1.0% 8.8% 3.5% 2.5%BDE 30BDE 33BDE 47 8.2% 6.6% 4.4% 1.8%BDE 49 3.7% 1.1%BDE 66 4.1%BDE 71 6.4% 6.3%BDE 75BDE 85BDE 99 8.0% 3.8%BDE 100 3.8% 4.2% 3.7% 3.4%BDE 116BDE 118BDE 119BDE 138BDE 153 5.7%BDE 154 12.4%BDE 155 10.8%BDE 156BDE 181BDE 183BDE 190BDE 191BDE 196BDE 197BDE 203BDE 205BDE 206BDE 207BDE 208BDE 209
6 

Table 29 (cont). Mussel Tissue XII (QA05TIS12): RSDs for three replicates - PBDEs

Lab 7 Lab 8 Lab 9 Lab 10 Lab 11 Lab 12

Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 Tissue XII SRM 2977 SRM 2978

rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd

BDE 15 5.5% 10.7%

BDE 17 19.8% 33.4% 11.7% 7.6%BDE 25 19.8% 33.4%BDE 28 3.2% 3.7% 5.0% 14.8% 12.2% 7.3%BDE 30BDE 33 5.0% 14.8%BDE 47 2.8% 0.4% 5.5% 17.2% 9.1% 9.6%BDE 49 4.7% 1.7% 14.7% 9.4%BDE 66 6.8% 8.2% 11.8% 11.9% 18.2% 19.1%BDE 71 5.4%BDE 75 14.7% 19.0%BDE 85 8.2% 35.0% 23.3% 6.8% 32.8%BDE 99 5.3% 11.0% 10.3% 12.9% 10.0% 8.7%BDE 100 2.3% 6.3% 6.4% 17.4% 10.1% 8.9%BDE 116BDE 118BDE 119 22.0% 61.9%BDE 138 57.0% 22.8%BDE 153 10.2% 37.8% 12.5% 14.4% 5.9% 7.9%BDE 154 15.4% 16.7% 11.8% 16.3% 11.8% 4.8%BDE 155 17.0% 45.0%BDE 156BDE 181 0.0%BDE 183 37.1% 25.5%BDE 190 0.0%BDE 191BDE 196BDE 197BDE 203 79.9% 71.5%BDE 205BDE 206BDE 207 0.0%BDE 208BDE 209
6 

Table 30. Marine Sediment XIII (QA05SED13): RSDs for three replicates - Water, TOC, and PAHs

Lab 1a Lab 1c Lab 2 Lab 3 Lab 4

Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1944 Sed XIII SRM 1941b Sed XIII SRM 1941b

rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd

Water 1.5% 1.7% 2.0% 6.2% 10.2% 1.2% 0.4% 3.1%TOC 4.5% 0.6%PAH ANALYSES Lab 1a Lab 1c Lab 2 Lab 3 Lab 4

Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1944 Sed XIII SRM 1941b Sed XIII SRM 1941b

rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd

naphthalene 1.1% 2.9% 28.7% 1.2% 8.7% 3.8% 29.5% 15.0% 2.1% 0.0%2-methylnaphthalene 2.5% 4.1% 23.8% 19.0% 15.0% 3.7% 11.8% 8.6% 2.1% 0.0%1-methylnaphthalene 3.9% 4.7% 18.0% 13.9% 13.0% 1.7% 14.9% 9.6% 0.5% 0.0%biphenyl 2.2% 1.2% 24.1% 3.6% 14.1% 2.9% 7.8% 6.0% 3.0% 0.0%2,6-dimethylnaphthalene 23.9% 3.4% 32.5% 42.3% 16.1% 3.8% 4.3% 0.0%acenaphthylene 3.6% 5.5% 16.1% 11.8% 8.3% 7.4% 6.4% 8.4% 5.2% 0.0%acenaphthene 3.3% 7.4% 17.6% 0.8% 2.5% 8.9% 2.8% 0.0%1,6,7-trimethylnaphthalene 14.4% 4.2% 6.5% 0.0%fluorene 2.1% 1.8% 38.8% 5.1% 20.9% 2.6% 5.5% 3.5% 5.2% 0.0%phenanthrene 4.0% 0.8% 29.4% 1.7% 18.0% 3.2% 5.0% 6.8% 3.0% 0.0%anthracene 3.0% 4.7% 24.8% 1.6% 19.5% 4.2% 12.0% 24.4% 3.1% 0.0%1-methylphenanthrene 1.8% 1.9% 12.6% 3.8% 29.8% 1.5% 2.9% 7.2% 2.6% 0.0%fluoranthene 0.7% 2.0% 21.4% 1.5% 16.1% 1.5% 5.9% 9.8% 5.1% 0.0%pyrene 2.0% 2.3% 21.0% 1.6% 16.3% 5.3% 8.9% 8.8% 4.4% 0.0%benz[a]anthracene 2.8% 6.2% 26.4% 2.4% 11.9% 3.3% 7.5% 15.4% 3.1% 0.0%chrysene 18.5% 2.1% 9.0% 1.5% 5.7% 12.0% 2.0% 0.0%triphenylene 17.3% 4.6%benzo[b ]fluoranthene 1.3% 1.5% 31.2% 25.5% 5.5% 11.5% 1.1% 0.0%benzo[j ]fluoranthene 2.5% 1.6%

benzo[k ]fluoranthene 3.9% 3.2% 16.2% 0.7% 4.3% 11.0% 6.5% 15.5% 2.2% 0.0%

benzo[e]pyrene 1.5% 2.5% 17.9% 2.8% 32.4% 6.9% 5.9% 12.2% 1.7% 0.0%benzo[a]pyrene 1.6% 3.3% 14.6% 5.6% 21.6% 10.9% 7.2% 25.3% 1.9% 0.0%perylene 1.1% 2.5% 17.8% 3.3% 26.2% 5.5% 6.3% 21.2% 0.9% 0.0%indeno[1,2,3-cd]pyrene 1.5% 3.9% 10.9% 11.0% 8.2% 16.0% 1.6% 0.0%dibenz[a,h]anthracene 11.3% 13.3% 17.5% 26.0% 1.9% 0.0%

benzo[ghi]perylene 1.6% 1.8% 8.3% 1.6% 6.4% 8.0% 13.4% 22.3% 1.6% 0.0%
6 

Table 30 (cont). Marine Sediment XIII (QA05SED13): RSDs for three replicates - Water, TOC, and PAHs

Lab 6 Lab 7 Lab 8 Lab 10 Lab 11 Lab 12

Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1941b

rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd

Water 1.8% 0.0% 0.0% 0.0% 0.0% 2.1% 4.2%TOC 8.7% 7.8% 1.0% 0.9% 0.0%PAH ANALYSES Lab 6 Lab 7 Lab 8 Lab 10 Lab 11 Lab 12

Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1941b

rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd

naphthalene 11.0% 1.8% 7.6% 19.8% 3.3% 0.8% 6.3% 3.5% 5.0% 9.1%2-methylnaphthalene 14.7% 2.9% 9.4% 16.6% 36.4% 8.4% 1.3% 5.8%1-methylnaphthalene 13.6% 4.0% 8.0% 10.5% 4.2% 6.4%biphenyl 11.2% 2.2% 7.4% 11.4% 7.1% 9.6% 9.3% 4.4%2,6-dimethylnaphthalene 15.4% 8.3% 6.6% 7.3% 3.7% 4.3%acenaphthylene 12.6% 7.7% 7.7% 4.0% 6.9% 40.4% 6.1% 2.7% 7.4% 4.3%acenaphthene 10.5% 3.6% 4.7% 0.0% 8.2% 65.5% 1.6% 3.9% 8.3% 8.4%1,6,7-trimethylnaphthalene 34.0% 18.8% 10.6% 6.3%fluorene 5.4% 4.9% 4.0% 7.2% 9.9% 33.4% 4.2% 8.5% 0.4% 4.4%phenanthrene 9.7% 3.7% 3.5% 3.7% 4.9% 8.1% 1.6% 3.4% 6.5% 5.3%anthracene 16.4% 4.8% 3.4% 8.5% 3.3% 3.4% 2.7% 1.1% 3.6% 1.8%1-methylphenanthrene 10.8% 4.1% 12.0% 10.6% 85.6% 0.5% 2.3% 1.5%fluoranthene 6.0% 1.1% 0.5% 3.5% 3.5% 23.0% 0.1% 3.9% 7.8% 1.0%pyrene 5.2% 1.8% 0.3% 4.7% 1.4% 25.7% 0.7% 3.0% 7.5% 6.2%benz[a]anthracene 6.1% 7.5% 3.3% 3.8% 6.7% 33.0% 7.6% 5.0%chrysene 6.1% 5.3% 1.5% 4.6% 3.3% 28.9% 2.3% 0.3% 6.1% 9.2%triphenylenebenzo[b ]fluoranthene 5.4% 2.9% 1.5% 3.4% 14.6% 45.8% 0.9% 8.8% 7.4% 10.4%

benzo[j]fluoranthenebenzo[k ]fluoranthene 4.5% 6.1% 0.8% 4.3% 8.6% 8.3% 4.3% 2.3% 15.1% 10.3%

benzo[e]pyrene 5.1% 2.6% 2.2% 4.3% 11.8% 23.9% 5.1% 2.5% 3.5% 7.5%benzo[a]pyrene 3.5% 17.1% 4.0% 2.5% 11.2% 33.1% 1.0% 3.8% 8.3% 5.7%perylene 5.0% 21.9% 1.9% 4.9% 6.6% 5.2%indeno[1,2,3-cd]pyrene 8.7% 23.2% 4.2% 8.9% 2.2% 32.0% 1.8% 71.6% 8.3% 6.8%dibenz[a,h]anthracene 7.1% 22.7% 3.0% 14.5% 30.8% 3.1% 5.4% 8.2%

benzo[ghi]perylene 12.3% 25.3% 3.2% 6.3% 3.4% 21.8% 0.2% 5.0% 7.6% 5.3%
6 

Table 31. Marine Sediment XIII (QA05SED13): RSDs for three replicates - Pesticides

Lab 1a Lab 1c Lab 2 Lab 3 Lab 4

Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1944 Sed XIII SRM 1941b Sed XIII SRM 1941b

rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd

alpha-HCH (a-BHC)hexachlorobenzene 0.4% 0.8% 7.0% 2.6% 7.5% 74.2% 0.9% 6.0%gamma-HCH (g-BHC,lindane) 4.3% 6.0%beta-HCH (b-BHC)heptachloraldrinheptachlor epoxideoxychlordane 14.7% 18.4%gamma-chlordane 0.5% 1.5% 50.4% 20.0% 4.4% 0.1%2,4'-DDE 4.1% 2.4% 0.0% 0.0%endosulfan I 0.0%cis-chlordane (alpha-chlordane) 2.4% 1.7% 24.9% 5.2% 3.1% 4.4%trans-nonachlor 1.1% 0.9% 3.5% 16.9%dieldrin4,4'-DDE 4.1% 3.3% 3.7% 4.9% 1.1% 2.4% 24.8% 8.5%2,4'-DDD 3.9% 30.7% 11.1% 19.3%endrinendosulfan II4,4'-DDD 1.9% 2.7% 6.5% 5.6% 12.9% 9.9%2,4'-DDT 20.6% 0.0%cis-nonachlor 14.3% 14.9% 2.1%4,4'-DDT 0.0%mirex 5.0% 9.6%endosulfan sulfatechlorpyrifos
6 

Table 31(cont). Marine Sediment XIII (QA05SED13): RSDs for three replicates - Pesticides

Lab 6 Lab 7 Lab 8 Lab 10 Lab 11 Lab 12

Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1941b

rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd

alpha-HCH (a-BHC) 2.8% 0.3% 16.4% 6.2%hexachlorobenzene 40.4% 1.0% 9.7% 5.7% 4.4% 3.4%gamma-HCH (g-BHC,lindane) 5.1% 16.3% 9.6%beta-HCH (b-BHC) 45.4% 2.4% 23.5% 5.9%heptachlor 21.4% 2.3%aldrin 6.6% 0.0% 0.0% 39.2%heptachlor epoxide 14.0% 1.3% 13.9% 1.7%oxychlordane 10.3% 18.5%gamma-chlordane 31.7% 52.0% 6.1% 5.1% 20.1% 6.1% 0.3%2,4'-DDE 35.8% 0.8% 0.8% 0.6% 6.7%endosulfan I 19.3% 20.9%cis-chlordane (alpha-chlordane) 18.0% 37.8% 4.6% 7.2% 5.6% 7.2% 4.1%trans-nonachlor 23.8% 22.4% 7.9% 0.0% 35.4% 10.5% 6.7% 4.9%dieldrin 30.6% 10.4% 12.1% 2.7% 2.5%4,4'-DDE 43.2% 6.8% 10.5% 1.2% 8.9% 2.4% 2.1% 4.5%2,4'-DDD 52.3% 141.3% 30.3% 33.5% 30.9%endrinendosulfan II 8.7% 9.6%4,4'-DDD 24.8% 12.0% 32.3% 1.7% 11.6% 15.1% 7.8%2,4'-DDT 2.0% 33.9% 11.3% 11.5%cis-nonachlor 23.3% 6.4% 41.7% 10.3% 3.2%4,4'-DDT 43.8% 0.0% 46.5% 39.2% 9.8% 6.9%mirex 32.4% 0.0%endosulfan sulfatechlorpyrifos
6 

Table 32. Marine Sediment XIII (QA05SED13): RSDs for three replicates - PCBs

Lab 1a Lab 1c Lab 2 Lab 3 Lab 4

Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1944 Sed XIII SRM 1941b Sed XIII SRM 1941b

rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd

PCB 8 3.8% 6.9% 2.3% 1.9% 17.6% 13.0%PCB 18 1.4% 4.1% 8.5% 6.1% 7.1% 4.7% 1.0% 6.4%PCB 28 1.1% 0.4% 6.1% 5.3% 13.1% 9.9% 0.6% 2.9%PCB 31 2.4% 1.3% 2.4% 7.2% 1.9% 8.7%PCB 44 5.7% 4.9% 2.0% 2.6% 8.0% 6.7% 1.4% 6.6%PCB 49 2.3% 3.8% 0.9% 3.8% 0.8% 7.2%PCB 52 2.6% 2.3% 5.1% 1.8% 10.3% 7.0% 1.8% 6.0%PCB 66 0.9% 1.3% 4.4% 7.2% 13.5% 9.0% 0.7% 6.5%PCB 95 2.1% 1.4% 9.0% 1.3% 4.0% 6.4%PCB 99 4.7% 3.9% 1.5% 3.7% 5.0% 7.1%PCB 101 2.8% 1.2% 7.0% 5.5% 11.9% 10.3% 4.7% 5.6%PCB 105 1.7% 3.9% 2.9% 7.5% 9.3% 15.1% 9.8% 5.4%PCB 118 0.9% 2.7% 3.2% 6.3% 13.6% 14.8% 6.1% 4.2%PCB 128 1.7% 2.4% 1.9% 9.7% 8.8% 21.6% 7.9% 4.5%PCB 138 7.6% 5.6% 4.5% 7.5% 2.6% 5.8%PCB 149 0.6% 1.7% 7.4% 4.0% 4.4% 7.5%PCB 153 5.1% 6.7% 4.0% 7.2%

PCB 156 1.5% 1.5% 3.4% 10.3%PCB 170 2.5% 4.2% 1.3% 3.9% 7.0% 16.4% 24.9% 9.8%PCB 180 3.3% 2.4% 9.5% 9.5% 6.1% 7.3% 27.5% 7.8%PCB 187 2.8% 4.3% 3.9% 4.3% 3.0% 5.5% 21.9% 8.7%PCB 194 7.0% 6.5% 27.4% 10.5%PCB 195 14.4% 7.5% 15.9% 0.0%PCB 206 5.5% 3.1% 10.7% 7.4% 5.3% 6.7% 4.4% 11.1%PCB 209 4.8% 2.6% 4.1% 9.6% 3.1% 7.2% 0.1% 12.6%
6 

Table 32 (cont). Marine Sediment XIII (QA05SED13): RSDs for three replicates - PCBs

Lab 6 Lab 7 Lab 8 Lab 10 Lab 11 Lab 12

Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1941b

rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd

PCB 8 23.4% 3.2% 9.5% 2.4% 5.2% 10.3% 0.1% 2.1% 17.6% 2.0% 11.3% 2.6%PCB 18 20.1% 3.5% 7.1% 2.4% 0.2% 10.5% 0.7% 2.2% 23.5% 19.9% 11.2% 4.1%PCB 28 23.3% 1.6% 6.0% 1.5% 5.7% 5.4% 0.1% 5.7% 5.8% 4.6% 6.4% 2.3%PCB 31 24.8% 4.7% 4.9% 0.2% 0.9% 1.8% 12.4% 6.8% 5.4% 4.6%PCB 44 22.3% 1.6% 2.9% 5.6% 22.3% 34.4% 0.5% 2.1% 13.5% 20.5% 6.0% 3.2%PCB 49 25.6% 2.9% 7.0% 6.3% 34.5% 42.0% 0.2% 3.2% 16.0% 18.6% 7.5% 1.8%PCB 52 24.1% 7.3% 4.4% 3.1% 22.2% 31.7% 0.5% 1.6% 17.1% 20.3% 6.4% 1.2%PCB 66 23.7% 3.2% 8.1% 5.0% 10.6% 13.1% 5.9% 3.4% 2.7% 2.4% 6.1% 5.9%PCB 95 26.7% 3.7% 7.4% 5.2% 8.4% 18.0% 8.7% 12.3% 6.2% 21.1% 9.6% 4.0%PCB 99 25.4% 5.0% 5.6% 6.6% 8.9% 16.0% 0.0% 1.3% 3.1% 10.6% 7.9% 1.5%PCB 101 26.6% 4.4% 4.7% 0.9% 10.6% 2.4% 0.1% 1.4% 0.8% 10.9% 19.6% 1.8%PCB 105 27.9% 4.3% 3.2% 3.1% 2.9% 4.2% 0.4% 1.1% 8.7% 4.8% 4.6% 3.0%PCB 118 26.0% 5.0% 6.6% 5.1% 0.2% 7.4% 0.5% 0.4% 7.9% 5.7% 4.0% 2.7%PCB 128 31.3% 13.6% 20.8% 8.3% 0.9% 10.2% 1.3% 20.6% 11.8% 11.0% 4.9% 3.2%PCB 138 25.9% 4.3% 4.7% 0.3% 3.7% 13.8% 0.5% 0.4% 14.8% 5.1% 4.4% 2.7%PCB 149 26.3% 3.3% 8.3% 7.0% 24.8% 34.2% 1.6% 0.7% 17.5% 13.3% 3.4% 2.9%PCB 153 25.9% 5.2% 2.9% 10.3% 16.3% 13.7% 0.4% 1.1% 15.5% 5.3% 7.8% 7.2%PCB 156 26.8% 1.1% 18.4% 5.7% 2.7% 7.7% 10.5% 3.1% 5.1% 2.4%PCB 170 23.9% 6.8% 9.8% 4.5% 9.0% 3.7% 0.7% 1.6% 13.7% 4.6% 2.9% 7.9%PCB 180 26.3% 6.3% 9.5% 4.5% 7.8% 0.4% 0.2% 2.5% 12.0% 7.0% 2.5% 1.8%PCB 187 109.5% 7.5% 6.3% 0.0% 6.9% 28.8% 0.2% 4.2% 15.7% 8.4% 4.9% 3.3%PCB 194 27.0% 9.6% 2.8% 0.7% 6.5% 2.3% 3.2% 13.6% 7.1% 6.9% 1.1%PCB 195 8.7% 0.0% 4.3% 3.5% 13.8% 6.8% 3.9% 5.7%PCB 206 29.7% 7.1% 4.5% 37.3% 20.9% 7.9% 3.9% 12.9% 1.8% 2.7% 1.8%PCB 209 3.4% 6.3% 3.4% 8.4% 0.7% 4.8% 13.5% 4.0% 2.9% 0.5%
6 

Table 33. Marine Sediment XIII (QA05SED13): RSDs for three replicates - PBDEs

Lab 1a Lab 1c Lab 2 Lab 3 Lab 4

Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1944 Sed XIII SRM 1941b Sed XIII SRM 1941b

rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd

BDE 15BDE 17BDE 25BDE 28BDE 30BDE 33BDE 47 3.4% 9.8%BDE 49BDE 66BDE 71BDE 75BDE 85BDE 99BDE 100BDE 116BDE 118BDE 119BDE 138BDE 153BDE 154BDE 155BDE 156BDE 181BDE 183BDE 190BDE 191BDE 196BDE 197BDE 203BDE 205BDE 206BDE 207BDE 208BDE 209
6 

Table 33 (cont). Marine Sediment XIII (QA05SED13): RSDs for three replicates - PBDEs

Lab 6 Lab 7 Lab 8 Lab 10 Lab 11 Lab 12

Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1941b Sed XIII SRM 1941b

rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd rsd

BDE 15 0.5% 9.3%BDE 17 5.6% 11.0%BDE 25BDE 28 13.0% 6.9% 1.9% 8.1%BDE 30BDE 33BDE 47 32.8% 19.3% 4.9% 11.9%BDE 49 1.1% 6.8%BDE 66 13.2% 17.5% 8.3% 12.7%BDE 71 8.4% 5.9%BDE 75 0.0%BDE 85 26.3% 76.8% 9.4% 14.5%BDE 99 20.0% 47.4% 2.1% 5.1%BDE 100 17.0% 27.6% 0.0% 3.2%BDE 116BDE 118BDE 119 35.4% 0.0%BDE 138 56.0% 0.0% 25.0% 29.4%BDE 153 7.0% 55.0% 4.0% 0.7%BDE 154 14.9% 62.6% 6.5% 2.9%BDE 155 6.0% 9.1%BDE 156BDE 181BDE 183BDE 190BDE 191BDE 196BDE 197BDE 203 92.0% 85.7%BDE 205BDE 206BDE 207BDE 208BDE 209 3.9% 4.3%
Table 34. Comparison of Concentrations for Marine Sediment XIII (QA05SED13) and SRM 1941b

Marine Sediment XIII SRM 1941b 

Exercise Assigned from Certificate

Value s Value 95% CI % difference

Water (percent) 47.4 3.2

TOC (percent) 2.70 0.58

Marine Sediment XIII SRM 1941b 

Exercise Assigned from Certificate

PAHs (ng/g dry mass) Value s Value 95% CI % difference

naphthalene 785 186 848 95 -7.4%

2-methylnaphthalene 219 66 276 53 -20.7%

1-methylnaphthalene 98.2 24.0 127 14 -22.7%

biphenyl 65.8 23.6 74 8 -11.1%

2,6-dimethylnaphthalene 81.3 43.6 75.9 4.5 7.1%

acenaphthylene 45.1 13.8 53.3 6.4 -15.4%

acenaphthene 28.9 8.4 38.4 5.2 -24.6%

1,6,7-trimethylnaphthalene no target 25.5 5.1

fluorene 56.1 12.2 85 15 -34.1%

phenanthrene 306 89 406 44 -24.5%

anthracene 137 47 184 18 -25.5%

1-methylphenanthrene 55.4 18.2 73.2 5.9 -24.4%

fluoranthene 496 140 651 50 -23.8%

pyrene 421 142 581 39 -27.5%

benz[a ]anthracene 241 80 335 25 -28.2%

chrysene 219 62 291 31 -24.7%

triphenylene no target 108 5

benzo[b ]fluoranthene 413 174 453 21 -8.8%

benzo[j ]fluoranthene no target 217 5

benzo[k ]fluoranthene 180 56 225 18 -20.0%

benzo[e]pyrene 286 97 325 25 -11.9%

benzo[a]pyrene 282 120 358 17 -21.3%

perylene 311 131 397 45 -21.6%

indeno[1,2,3-cd]pyrene 258 101 341 57 -24.4%

dibenz[a,h]anthracene 41.9 21.2 53 10 -20.9%

benzo[ghi]perylene 244 96 307 45 -20.5%

Note: Bolded values are certified concentrations while other values for SRM 1941b are reference values
6 

Table 34 (cont). Comparison of Concentrations for Marine Sediment XIII (QA05SED13) and SRM 1941b

Marine Sediment XIII SRM 1941b 

Pesticides (ng/g dry mass) Exercise Assigned from Certificate

Value s Value 95% CI % difference

alpha-HCH (a-BHC) no target no target

hexachlorobenzene 5.38 1.90 5.83 0.38 -7.8%

gamma-HCH (g-BHC,lindane) no target no target

beta-HCH (b-BHC) no target no target

heptachlor no target no target

aldrin no target no target

heptachlor epoxide no target no target

oxychlordane no target no target

gamma-chlordane 0.572 0.148 0.566 0.093 1.1%

2,4'-DDE 0.380 0.103 0.38 0.12 0.1%

endosulfan I no target no target

cis-chlordane (alpha-chlordane) 0.482 0.058 0.85 0.11 -43.2%

trans-nonachlor 0.286 0.089 0.438 0.073 -34.8%

dieldrin 0.386 0.034 no target

4,4'-DDE 3.44 1.08 3.22 0.28 7.0%

2,4'-DDD 0.927 0.624 no target

endrin no target no target

endosulfan II no target no target

4,4'-DDD 4.18 1.42 4.66 0.46 -10.3%

2,4'-DDT no target no target

cis-nonachlor 0.454 0.493 0.378 0.053 20.0%

4,4'-DDT 0.537 0.174 1.12 0.42 -52.1%

mirex no target no target

endosulfan sulfate no target no target

chlorpyrifos no target no target

Note: Bolded values are certified concentrations while other values for SRM 1941b are reference values
6 

Table 34 (cont). Comparison of Concentrations for Marine Sediment XIII (QA05SED13) and SRM 1941b

Marine Sediment XIII SRM 1941b 

PCBs (ng/g dry mass) Exercise Assigned from Certificate

Value s Value 95% CI % difference

PCB 8 1.36 0.42 1.65 0.19 -17.3%

PCB 18 2.04 0.48 2.39 0.29 -14.5%

PCB 28 3.79 0.97 4.52 0.57 -16.1%

PCB 31 2.83 0.94 3.18 0.41 -11.0%

PCB 44 3.47 0.99 3.85 0.2 -9.8%

PCB 49 3.64 1.22 4.34 0.28 -16.1%

PCB 52 4.48 1.39 5.24 0.28 -14.5%

PCB 66 4.32 1.41 4.96 0.53 -12.9%

PCB 95 3.44 1.25 3.93 0.62 -12.5%

PCB 99 2.45 0.87 2.9 0.36 -15.5%

PCB 101 4.86 1.36 5.11 0.34 -4.8%

PCB 105 1.26 0.40 1.43 0.1 -12.0%

PCB 118 3.59 1.26 4.23 0.19 -15.1%

PCB 128 0.684 0.275 0.696 0.044 -1.8%

PCB 138 3.83 1.23 3.6 0.28 6.3%

PCB 149 4.18 1.44 4.35 0.26 -3.8%

PCB 153 4.99 2.46 5.47 0.32 -8.8%

PCB 156 0.444 0.128 0.507 0.09 -12.3%

PCB 170 1.19 0.36 1.35 0.09 -11.5%

PCB 180 2.97 1.15 3.24 0.51 -8.2%

PCB 187 2.20 0.69 2.17 0.22 1.2%

PCB 194 1.01 0.50 1.04 0.06 -2.8%

PCB 195 0.291 0.166 0.645 0.06 -54.9%

PCB 206 1.91 0.66 2.42 0.19 -21.1%

PCB 209 4.02 1.47 4.86 0.45 -17.3%

Note: Bolded values are certified concentrations while other values for SRM 1941b are reference values
6 

0

50

100

150

200

250

phen fluor B[a]A B[a]P B[ghi]P

conc (ng/g dry mass)

Tis12

CCQM P40

0

20

40

60

80

100

120

140

PCB 28 PCB 101 PCB 105 PCB 153 PCB 170

conc (ng/g dry mass)

Tis12

CCQM P40

0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

45.0

t-nonachlor 4,4'-DDE 4,4'-DDT

conc (ng/g dry mass)

Tis12

CCQM P40

Figure 1. Comparison of Concentrations for Mussel Tissue XII as determined in this study and in 

a CCQM pilot study for selected analytes. Shown are the assigned values and associated 

uncertainties from each study. 
6 

Appendix A: Description, Storage, Use, and Reporting Instructions 

for Mussel Tissue XII (QA05TIS12) 
6 

NIST Intercomparison Exercise Program for 

Organic Contaminants in the Marine Environment 

NIST QA Program

Intercomparison Exercise: Mussel Tissue XII 

Description of Materials and Instructions

Intercomparison Exercise Materials:

QA05TIS12 (Mussel Tissue XII)

The one jar contains approximately 8 g (dry-mass basis) of Mussel Tissue XII. This freeze-dried 

material was prepared from mussels collected from an urban area. This material has not been 

enriched or spiked. Each 30-mL amber jar has a Teflon-lined screw cap and is labeled with an 

individual jar number as well as the above name. 

It is requested that three concurrent analyses of SRM 2977 Mussel Tissue (Organic 

Contaminants and Trace Elements) are also performed. This material can be obtained from the 

NIST Standard Reference Materials Program ($502/10 g (dry-mass basis) (phone: 301/975-

6776; fax: 301/948-3730). See the following link for information on ordering on-line: 

https://srmors.nist.gov/view_detail.cfm?srm=2977. 

Storage of Materials:

Mussel Tissue Material. The tissue material should be stored in the dark at room temperature. If 

only a portion of the contents of a jar is used, the jar should be tightly closed immediately after 

removal of a subsample to preserve the integrity of the remaining material for later analysis. 

Instructions for Use: 

You are to analyze Mussel Tissue XII and SRM 2977, using your laboratory's and/or program's 

analytical protocols, for the concentrations (mass/mass [dry-mass basis]) of the 26 polycyclic 

aromatic hydrocarbon (PAH) compounds, 25 chlorinated pesticides, 25 polychlorinated biphenyl 

(PCB) congeners, and 34 polybrominated diphenyl ether (PBDE) congeners1

 of interest in the 

current NIST Intercomparison Exercise Program for Organic Contaminants in the Marine 

Environment. These compounds are listed in Table 1. 

 

1

If your laboratory is not analyzing samples for all four chemical classes, you are expected to submit 

results only for those compounds currently being determined in your laboratory. 
6 

The percentage of total extractable organics (or lipid) in Mussel Tissue XII and SRM 2977 

should also be determined. You should have received sufficient material for this purpose. The 

amount of material used for each analysis should correspond to the amount (dry-mass basis) of 

marine tissue that you would typically analyze as prescribed in your protocols. 

You should analyze three samples of Mussel Tissue XII and at least one or more samples of 

SRM 2977 in three different batches using your protocol for tissue samples. Specifically, we are 

asking that you analyze one sample of Mussel Tissue XII and one sample of SRM 2977 with one 

batch of laboratory samples; analyze a second sample of each material with another batch; and 

the third sample with yet another batch. This will allow a more realistic assessment of laboratory 

precision over a longer term than the assessment obtained when a laboratory places all three 

samples in the same extraction and cleanup batch and the resulting extracts are analyzed using 

the same calibration curve, etc. 

Reporting of Results: 

Please report one result, as if three figures were significant, for each of the requested analytes in 

each of the three replicates of the Mussel Tissue XII and of SRM 2977. Report results in units of 

ng/g dry-mass basis. Report the date of measurement of each sample in the requested m/d/y 

format. 

We recognize that the reported concentrations for some of the requested determinands will 

probably include concentrations of compounds reported to coelute with the determinand of 

interest with methods commonly in use in environmental laboratories. Please note at the bottom 

of your table of reported results if any coelution qualifiers are applicable to your data. Please 

note that any changes you make to the column or row headings within the tables will not be seen 

by the coordinators because only the table entries and comments at the bottom of the tables are 

automatically transferred to the exercise database. 

We prefer that concentration values be reported for each analyte determined. If the measured 

concentration is below your typical reporting concentration for an analyte in a particular matrix, 

you can report the number and list the appropriate detection limit, quantification limit, etc. at the 

bottom of the data table. However, if you need to report non-numerical data please use the 

following conventions: 

NA "Not analyzed", "not determined" 

<"value" "Less than specified concentration", e.g., <8 ng/g 

Other "Other"; add note of explanation at end of data table, e.g., interference 

DL "Below detection limit" may be used, however, <"value" is preferable 

Do not use negative numbers or parentheses to indicate "less than detection limits". 
The attached file is an EXCEL file, TIS12.xls. If you have any software/hardware conversion 

problems, please contact Michele Schantz. The data file templates also include places for you to 

list the surrogate/internal standards and type of calibration curve used, and to provide a brief 

description of the analyses. Please do not add spaces before entering numbers in the table cells 

and enter them as "numbers" not as "labels". Please do not insert any columns or rows within

the table in the data file. If you wish to include additional data and/or other information or 

comments, you may add it to the bottom of the data table in the diskette file or send it in hard 

copy. A printout of the data file format is shown in Table 2. 

Submit your results by December 15, 2005 as an attached file via e-mail to: 

E-mail: michele.schantz@nist.gov 

Further Information:

If you need further information, please contact Michele at the following address or phone 

numbers: 

Michele M. Schantz 

NIST 

100 Bureau Drive Stop 8392 

 Gaithersburg, MD 20899-8392 

Phone: (301)975-3106 

FAX: (301)977-0685 
Table 1: Analytes of Interest in NIST Intercomparison Exercise Program for Organic 

Contaminants in the Marine Environment 

Chlorinated Pesticides 

hexachlorobenzene 2,4'-DDE 

alpha-HCH (alpha-BHC) 4,4'-DDE 

beta-HCH (beta-BHC) 2,4'-DDD 

gamma-HCH (gamma-BHC, Lindane) 4,4'-DDD 

heptachlor 2,4'-DDT 

heptachlor epoxide 4,4'-DDT 

cis-chlordane (alpha-chlordane) chlorpyrifos 

trans-chlordane (gamma-chlordane) aldrin 

oxychlordane dieldrin 

cis-nonachlor endrin 

trans-nonachlor endosulfan I 

mirex endosulfan II 

endosulfan sulfate 

Polychlorinated Biphenyl Congeners

 PCB No. Compound Name

 8 2,4'-dichlorobiphenyl 

 18 2,2',5-trichlorobiphenyl 

 28 2,4,4'-trichlorobiphenyl 

 31 2,4',5-trichlorobiphenyl 

 44 2,2',3,5'-tetrachlorobiphenyl 

 49 2,2',4,5'-tetrachlorobiphenyl 

 52 2,2',5,5'-tetrachlorobiphenyl 

 66 2,3',4,4'-tetrachlorobiphenyl 

 95 2,2',3,5',6-pentachlorobiphenyl 

 99 2,2',4,4',5-pentachlorobiphenyl 

 101 2,2',4,5,5'-pentachlorobiphenyl 

 105 2,3,3',4,4'-pentachlorobiphenyl 

 118 2,3',4,4',5-pentachlorobiphenyl 

 128 2,2',3,3',4,4'-hexachlorobiphenyl 

 138 2,2',3,4,4',5'-hexachlorobiphenyl 

 149 2,2',3,4',5',6-hexachlorobiphenyl 

 153 2,2',4,4',5,5'-hexachlorobiphenyl 

 156 2,3,3',4,4',5-hexachlorobiphenyl 

 170 2,2',3,3',4,4',5-heptachlorobiphenyl 

 180 2,2',3,4,4',5,5'-heptachlorobiphenyl 

 187 2,2',3,4',5,5',6-heptachlorobiphenyl 

 194 2,2',3,3',4,4',5,5'-octachlorobiphenyl 

 195 2,2',3,3',4,4',5,6-octachlorobiphenyl 

 206 2,2',3,3',4,4',5,5',6-nonachlorobiphenyl 

 209 decachlorobiphenyl 
Table 1. (continued) 

Polycyclic aromatic hydrocarbons (PAH)

naphthalene benz[a]anthracene 

2-methylnaphthalene chrysene 

1-methylnaphthalene triphenylene 

biphenyl benzo[b]fluoranthene 

2,6-dimethylnaphthalene benzo[j]fluoranthene 

acenaphthylene benzo[k]fluoranthene 

acenaphthene benzo[e]pyrene 

1,6,7-trimethylnaphthalene benzo[a]pyrene 

fluorene perylene 

phenanthrene indeno[1,2,3-cd]pyrene 

anthracene dibenz[a,h]anthracene 

1-methylphenanthrene benzo[ghi]perylene 

fluoranthene 

pyrene

Polybrominated diphenyl ethers (PBDEs)

 BDE 15 (4,4’-dibromo-) BDE 138 (2,2’,3,4,4’,5’-hexabromo-) 

 BDE 17 (2,2’,4-tribromo-) BDE 153 (2,2’,4,4’,5,5’-hexabromo-) 

 BDE 25 (2,3’,4-tribromo-) BDE 154 (2,2’,4,4’,5,6’-hexabromo-) 

 BDE 28 (2,4,4’-tribromo-) BDE 155 (2,2’,4,4’,6,6’-hexabromo-) 

 BDE 30 (2,4,6-tribromo-) BDE 156 (2,3,3’,4,4’,5-hexabromo-) 

 BDE 33 (2’,3,4-tribromo-) BDE 181 (2,2’,3,4,4’,5,6-heptabromo-) 

 BDE 47 (2,2’,4,4’-tetrabromo-) BDE 183 (2,2’,3,4,4’,5’,6-heptabromo-) 

 BDE 49 (2,2’,4,5’-tetrabromo-) BDE 190 (2,3,3’,4,4’,5,6-heptabromo-) 

 BDE 66 (2,3’,4,4’-tetrabromo-) BDE 191 (2,3,3’,4,4’,5,6’-heptabromo-) 

 BDE 71 (2,3’,4’,6-tetrabromo-) BDE 196 (2,2’,3,3’,4,4’,5,6’-octabromo-) 

 BDE 75 (2,4,4’,6-tetrabromo-) BDE 197 (2,2’,3,3’,4,4’,6,6’-octabbromo-) 

 BDE 85 (2,2’,3,4,4’-pentabromo-) BDE 203 (2,2’,3,4,4’,5,5’,6-octabromo-) 

 BDE 99 (2,2’,4,4’,5-pentabromo-) BDE 205 (2,3,3’,4,4’,5,5’,6-octabromo-) 

 BDE 100 (2,2’,4,4’,6-pentabromo-) BDE 206 (2,2’,3,3’,4,4’,5,6,6’-nonabromo-) 

 BDE 116 (2,3,4,5,6-pentabromo-) BDE 207 (2,2’3,3’,4,4’,5,6,6’-nonabromo-) 

 BDE 118 (2,3’,4,4’,5-pentabromo-) BDE 208 (2,2’,3,3’,4,5,5’,6,6’-nonabromo-) 

 BDE 119 (2,3’,4,4’,6-pentabromo-) BDE 209 (decabromo-)
 

Table 2. Diskette Data File Format (File: TIS12.*)

NIST Intercomparison Exercise Program for Organics in the Marine Environment

NIST QA Program

Sample: QA05TIS12 - Mussel Tissue XII

Please fill in all blanks; Use requested units of concentration; Report results as if 3 figures were significant

DO NOT INSERT ROWS OR COLUMNS WITHIN THIS TABLE. DO NOT MOVE CELLS.

 - If necessary, add additional data/information at the end of the table.

 - Use one of the following if no concentration is reported for an analyte:

 NA = Not analyzed/determined; <"conc" = <detection limit conc.; Other = other, explain in a note at end of table

 (DL = "below detection limit" may be used, but <"conc", e.g., <8, is preferable.)

 Do not use parentheses or negative numbers to indicate "less than detection limit".

Reporting Date (m/d/y): _______________________

Laboratory: _______________________

Submitted by: _______________________

BRIEF DESCRIPTION OF PROCEDURES USED:

Approximate amount of sample extracted: 

Mussel XII ___________ g, dry basis; SRM 2977 ___________ g, dry basis

Method used for determining percentage Total Extractable Organics (TEO) or lipid:

______________________________________________________________________

______________________________________________________________________

______________________________________________________________________

Extraction method: ____________________________________________________________________________________________

Extraction solvent: ____________________________________________________________________________________________

Extraction time: ____________________________________________________________________________________________

Extraction - other: ____________________________________________________________________________________________

____________________________________________________________________________________________

Sample extract cleanup method: ____________________________________________________________________________________________

____________________________________________________________________________________________

____________________________________________________________________________________________

____________________________________________________________________________________________

____________________________________________________________________________________________

Analytical method used (e.g., GC-FID, GC-ECD):

Analyt. Instr. Column Phase Col. Length, m Col. i.d., mm Col. film thickness, μm

PAH ______________________________________________________________________

 Pesticides ______________________________________________________________________

 PCB Congeners ______________________________________________________________________

 BDE Congeners ______________________________________________________________________

Method of quantitation (IS = internal standard, ES = external standard):

 PAH __________________

 Pesticides __________________

 PCB Congeners __________________

 BDE Congeners __________________

IF internal standard method was used, please complete the following section:

 Identity of internal standards/surrogates used that were:

 Added PRIOR to extraction of sample:

 PAH ________________________________________________________________________

 Pesticides ________________________________________________________________________

 PCB Congeners ________________________________________________________________________

BDE Congeners ________________________________________________________________________

 Added after extraction/cleanup and JUST PRIOR to chromatographic analysis:

PAH ________________________________________________________________________

Pesticides ________________________________________________________________________

 PCB Congeners ________________________________________________________________________

BDE Congeners ________________________________________________________________________

 Any others? Added at what point in analyses__________________________________________________________

PAH ________________________________________________________________________

Pesticides ________________________________________________________________________

 PCB Congeners ________________________________________________________________________

BDE Congeners ________________________________________________________________________

 IS/surrogate standards used for quantitation calculations were: 

________ those added prior to extraction

________ those added after extraction/cleanup and just prior to chromatographic analysis

 If the IS/surrogates added after extraction/cleanup extraction were used for quantitation, 

 were results corrected for percent recovery? __________________
 

 Percent recovery range: PAH __________________

Pesticides __________________

PCB Congeners __________________

BDE Congeners __________________

Calibration Curve

Analytes outside of calibration 

Points Conc. Range curve calibration range

 PAH _______________________________ __________________________________

 Pesticides _______________________________ __________________________________

 PCB Congeners _______________________________ __________________________________

 BDE Congeners _______________________________ __________________________________

Were PCB congeners separated from pesticides prior to GC? __________________

Please note any differences in procedures used for SRM 2977 analyses from those for Mussel Tissue XII described above:

_____________________________________________________________________________________________________________________

_____________________________________________________________________________________________________________________

_____________________________________________________________________________________________________________________

_____________________________________________________________________________________________________________________

_____________________________________________________________________________________________________________________

RESULTS:

PERCENT Total Extractable Organics (TEO) or Lipid (List each result if determined more than once. Enter results as a number, for example 90.0. 

DO NOT change format of cell to percent.)

Tissue XII Tissue XII Tissue XII SRM 2977 SRM 2977 SRM 2977

(percent) (percent) (percent) (percent) (percent) (percent)

TEO or lipid __________ __________ __________ __________ __________ __________ 

PAH ANALYSES Tissue XII Tissue XII Tissue XII SRM 2977 SRM 2977 SRM 2977

Batch A Batch B Batch C Batch A Batch B Batch C

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

Analyst (Initials) __________ __________ __________ __________ __________ __________ 

Date(s) of measurements (m/d/y) __________ __________ __________ __________ __________ __________ 

Sample Jar number __________ __________ __________ 

Tissue XII Tissue XII Tissue XII SRM 2977 SRM 2977 SRM 2977

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

(ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass)

naphthalene __________ __________ __________ __________ __________ __________ 

2-methylnaphthalene __________ __________ __________ __________ __________ __________ 

1-methylnaphthalene __________ __________ __________ __________ __________ __________ 

biphenyl __________ __________ __________ __________ __________ __________ 

2,6-dimethylnaphthalene __________ __________ __________ __________ __________ __________ 

acenaphthylene __________ __________ __________ __________ __________ __________ 

acenaphthene __________ __________ __________ __________ __________ __________ 

1,6,7-trimethylnaphthalene __________ __________ __________ __________ __________ __________ 

fluorene __________ __________ __________ __________ __________ __________ 

phenanthrene __________ __________ __________ __________ __________ __________ 

anthracene __________ __________ __________ __________ __________ __________ 

1-methylphenanthrene __________ __________ __________ __________ __________ __________ 

fluoranthene __________ __________ __________ __________ __________ __________ 

pyrene __________ __________ __________ __________ __________ __________ 

benz[a]anthracene __________ __________ __________ __________ __________ __________ 

chrysene __________ __________ __________ __________ __________ __________ 

triphenylene __________ __________ __________ __________ __________ __________ 

benzo[b ]fluoranthene __________ __________ __________ __________ __________ __________ 

benzo[j ]fluoranthene __________ __________ __________ __________ __________ __________ 

benzo[k ]fluoranthene __________ __________ __________ __________ __________ __________ 

benzo[e]pyrene __________ __________ __________ __________ __________ __________ 

benzo[a]pyrene __________ __________ __________ __________ __________ __________ 

perylene __________ __________ __________ __________ __________ __________ 

indeno[1,2,3-cd]pyrene __________ __________ __________ __________ __________ __________ 

dibenz[a,h]anthracene __________ __________ __________ __________ __________ __________ 

benzo[ghi]perylene __________ __________ __________ __________ __________ __________ 
 

PESTICIDE ANALYSES Tissue XII Tissue XII Tissue XII SRM 2977 SRM 2977 SRM 2977

Batch A Batch B Batch C Batch A Batch B Batch C

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

Analyst (Initials) __________ __________ __________ __________ __________ __________ 

Date(s) of measurements (m/d/y) __________ __________ __________ __________ __________ __________ 

Sample Jar number __________ __________ __________ 

Tissue XII Tissue XII Tissue XII SRM 2977 SRM 2977 SRM 2977

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

(ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass)

alpha-HCH (a-BHC) __________ __________ __________ __________ __________ __________ 

hexachlorobenzene __________ __________ __________ __________ __________ __________ 

gamma-HCH (g-BHC,lindane) __________ __________ __________ __________ __________ __________ 

beta-HCH (b-BHC) __________ __________ __________ __________ __________ __________ 

heptachlor __________ __________ __________ __________ __________ __________ 

aldrin __________ __________ __________ __________ __________ __________ 

heptachlor epoxide __________ __________ __________ __________ __________ __________ 

oxychlordane __________ __________ __________ __________ __________ __________ 

gamma-chlordane __________ __________ __________ __________ __________ __________ 

2,4'-DDE __________ __________ __________ __________ __________ __________ 

endosulfan I __________ __________ __________ __________ __________ __________ 

cis-chlordane (alpha-chlordane) __________ __________ __________ __________ __________ __________ 

trans-nonachlor __________ __________ __________ __________ __________ __________ 

dieldrin __________ __________ __________ __________ __________ __________ 

4,4'-DDE __________ __________ __________ __________ __________ __________ 

2,4'-DDD __________ __________ __________ __________ __________ __________ 

endrin __________ __________ __________ __________ __________ __________ 

endosulfan II __________ __________ __________ __________ __________ __________ 

4,4'-DDD __________ __________ __________ __________ __________ __________ 

2,4'-DDT __________ __________ __________ __________ __________ __________ 

cis-nonachlor __________ __________ __________ __________ __________ __________ 

4,4'-DDT __________ __________ __________ __________ __________ __________ 

mirex __________ __________ __________ __________ __________ __________ 

endosulfan sulfate __________ __________ __________ __________ __________ __________ 

chlorpyrifos __________ __________ __________ __________ __________ __________ 

PCB CONGENER ANALYSES Tissue XII Tissue XII Tissue XII SRM 2977 SRM 2977 SRM 2977

Batch A Batch B Batch C Batch A Batch B Batch C

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

Analyst (Initials) __________ __________ __________ __________ __________ __________ 

Date(s) of measurements (m/d/y) __________ __________ __________ __________ __________ __________ 

Sample Jar number __________ __________ __________ 

Tissue XII Tissue XII Tissue XII SRM 2977 SRM 2977 SRM 2977

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

(ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass)

PCB 8 __________ __________ __________ __________ __________ __________ 

PCB 18 __________ __________ __________ __________ __________ __________ 

PCB 28 __________ __________ __________ __________ __________ __________ 

PCB 31 __________ __________ __________ __________ __________ __________ 

PCB 44 __________ __________ __________ __________ __________ __________ 

PCB 49 __________ __________ __________ __________ __________ __________ 

PCB 52 __________ __________ __________ __________ __________ __________ 

PCB 66 __________ __________ __________ __________ __________ __________ 

PCB 95 __________ __________ __________ __________ __________ __________ 

PCB 99 __________ __________ __________ __________ __________ __________ 

PCB 101 __________ __________ __________ __________ __________ __________ 

PCB 105 __________ __________ __________ __________ __________ __________ 

PCB 118 __________ __________ __________ __________ __________ __________ 

PCB 128 __________ __________ __________ __________ __________ __________ 

PCB 138 __________ __________ __________ __________ __________ __________ 

PCB 149 __________ __________ __________ __________ __________ __________ 

PCB 153 __________ __________ __________ __________ __________ __________ 

PCB 156 __________ __________ __________ __________ __________ __________ 

PCB 170 __________ __________ __________ __________ __________ __________ 

PCB 180 __________ __________ __________ __________ __________ __________ 

PCB 187 __________ __________ __________ __________ __________ __________ 

PCB 194 __________ __________ __________ __________ __________ __________ 

PCB 195 __________ __________ __________ __________ __________ __________ 

PCB 206 __________ __________ __________ __________ __________ __________ 

PCB 209 __________ __________ __________ __________ __________ __________ 
 

BDE CONGENER ANALYSES Tissue XII Tissue XII Tissue XII SRM 2977 SRM 2977 SRM 2977

Batch A Batch B Batch C Batch A Batch B Batch C

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

Analyst (Initials) __________ __________ __________ __________ __________ __________ 

Date(s) of measurements (m/d/y) __________ __________ __________ __________ __________ __________ 

Sample Jar number __________ __________ __________ 

Tissue XII Tissue XII Tissue XII SRM 2977 SRM 2977 SRM 2977

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

(ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass)

BDE 15 __________ __________ __________ __________ __________ __________ 

BDE 17 __________ __________ __________ __________ __________ __________ 

BDE 25 __________ __________ __________ __________ __________ __________ 

BDE 28 __________ __________ __________ __________ __________ __________ 

BDE 30 __________ __________ __________ __________ __________ __________ 

BDE 33 __________ __________ __________ __________ __________ __________ 

BDE 47 __________ __________ __________ __________ __________ __________ 

BDE 49 __________ __________ __________ __________ __________ __________ 

BDE 66 __________ __________ __________ __________ __________ __________ 

BDE 71 __________ __________ __________ __________ __________ __________ 

BDE 75 __________ __________ __________ __________ __________ __________ 

BDE 85 __________ __________ __________ __________ __________ __________ 

BDE 99 __________ __________ __________ __________ __________ __________ 

BDE 100 __________ __________ __________ __________ __________ __________ 

BDE 116 __________ __________ __________ __________ __________ __________ 

BDE 118 __________ __________ __________ __________ __________ __________ 

BDE 119 __________ __________ __________ __________ __________ __________ 

BDE 138 __________ __________ __________ __________ __________ __________ 

BDE 153 __________ __________ __________ __________ __________ __________ 

BDE 154 __________ __________ __________ __________ __________ __________ 

BDE 155 __________ __________ __________ __________ __________ __________ 

BDE 156 __________ __________ __________ __________ __________ __________ 

BDE 181 __________ __________ __________ __________ __________ __________ 

BDE 183 __________ __________ __________ __________ __________ __________ 

BDE 190 __________ __________ __________ __________ __________ __________ 

BDE 191 __________ __________ __________ __________ __________ __________ 

BDE 196 __________ __________ __________ __________ __________ __________ 

BDE 197 __________ __________ __________ __________ __________ __________ 

BDE 203 __________ __________ __________ __________ __________ __________ 

BDE 205 __________ __________ __________ __________ __________ __________ 

BDE 206 __________ __________ __________ __________ __________ __________ 

BDE 207 __________ __________ __________ __________ __________ __________ 

BDE 208 __________ __________ __________ __________ __________ __________ 

BDE 209 __________ __________ __________ __________ __________ __________ 

(Any additional data/information should be added here.)
 

Appendix B: Description, Storage, Use, and Reporting Instructions 

for Marine Sediment XIII (QA05SED13) 
 

NIST Intercomparison Exercise Program for 

Organic Contaminants in the Marine Environment 

NIST QA Program

Intercomparison Exercise: Marine Sediment XIII 

Description of Materials and Instructions

Intercomparison Exercise Materials:

QA05SED13 (Marine Sediment XIII)

Each of the three jars contains approximately 21 g (wet basis) of Marine Sediment XII. This 

wetted sediment was prepared from material that was collected from a harbor area in the 

northeastern section of the US coast and then freeze-dried, ground sieved, and radiationsterilized. This material has not been enriched or spiked. Each 2-oz clear glass jar has a 

Teflon-lined screw cap and is labeled with an individual jar number as well as the above name. 

It is requested that three concurrent analyses of SRM 1941b Organics in Marine Sediment are 

also performed. This material can be obtained from the NIST Standard Reference Materials 

Program ($524/50 g (dry-mass basis) (phone: 301/975-6776; fax: 301/948-3730). See the 

following link for information on ordering on-line: 

https://srmors.nist.gov/view_detail.cfm?srm=1941B. 

Storage of Materials:

Marine Sediment Material. This Marine Sediment XIII material should be stored in the dark at 

temperatures of -15  ̊C or lower. If only a portion of the contents of a jar is used, that jar should 

be tightly closed immediately after removal of a subsample to preserve the integrity of the 

remaining material for later analysis. 

Instructions for Use: 

You are to analyze Marine Sediment XIII and SRM 1941b, using your laboratory's and/or 

program's analytical protocols, for the concentrations (mass/mass [dry-mass basis]) of the 26 

polycyclic aromatic hydrocarbon (PAH) compounds, 25 chlorinated pesticides, 25 

polychlorinated biphenyl (PCB) congeners, and 34 polybrominated diphenyl ether (PBDE) 

congeners2

 of interest in the current NIST Intercomparison Exercise Program for Organic 

Contaminants in the Marine Environment. These compounds are listed in Table 1. 

 

2

If your laboratory is not analyzing samples for all chemical classes, you are expected to submit results 

only for those compounds currently being determined in your laboratory. 
 

The percentage of water in Sediment XIII should be determined so that the results can be 

reported on a dry basis. You should have received sufficient material so that you can perform 

separate determinations for the water content if you do not dry your sediment samples prior to 

analysis. In addition, the percentage of total organic carbon should be determined in Sediment 

XIII and SRM 1941b. 

The amount of material used for each analysis should correspond to the amount (wet basis) of 

marine sediment that you would typically analyze as prescribed in your protocols. Prior to 

removing an aliquot of Sediment XIII, you should thaw the sample in the jar and then stir or 

otherwise mix it thoroughly. 

You should analyze three samples of Marine Sediment XIII and at least one or more samples of 

SRM 1941b in three different batches using your protocol for marine sediment samples. 

Specifically, we are asking that you analyze one sample of Sediment XIII and one sample of 

SRM 1941b with one batch of laboratory samples; analyze a second sample of each material 

with another batch; and the third sample with yet another batch. This will allow a more realistic 

assessment of laboratory precision over a longer term than the assessment obtained when a 

laboratory places all three samples in the same extraction and cleanup batch and the resulting 

extracts are analyzed using the same calibration curve, etc. 

Reporting of Results: 

Please report one result, as if three figures were significant, for each of the requested analytes in 

each of the three replicates of the Marine Sediment XIII and of SRM 1941b. Report results in 

units of ng/g dry-mass basis. Report the date of measurement of each sample in the requested 

m/d/y format. Also, report the results of your percentage water determinations of Marine 

Sediment XIII. 

We recognize that the reported concentrations for some of the requested determinands will 

probably include concentrations of compounds reported to coelute with the determinand of 

interest with methods commonly in use in environmental laboratories. Please note at the bottom 

of your table of reported results if any coelution qualifiers are applicable to your data. Please 

note that any changes you make to the column or row headings within the tables will not be seen 

by the coordinators because only the table entries and comments at the bottom of the tables are 

automatically transferred to the exercise database. 

We prefer that concentration values be reported for each analyte determined. If the measured 

concentration is below your typical reporting concentration for an analyte in a particular matrix, 

you can report the number and list the appropriate detection limit, quantification limit, etc. at the 

bottom of the data table. However, if you need to report non-numerical data please use the 

following conventions: 

NA "Not analyzed", "not determined" 

<"value" "Less than specified concentration", e.g., <8 ng/g 
 

Other "Other"; add note of explanation at end of data table, e.g., interference 

DL "Below detection limit" may be used, however, <"value" is preferable 

Do not use negative numbers or parentheses to indicate "less than detection limits". 

The attached file is an EXCEL file, SED13.xls. If you have any software/hardware conversion 

problems, please contact Michele Schantz. The data file templates also include places for you to 

list the surrogate/internal standards and type of calibration curve used, and to provide a brief 

description of the analyses. Please do not add spaces before entering numbers in the table cells 

and enter them as "numbers" not as "labels". Please do not insert any columns or rows within

the table in the data file. If you wish to include additional data and/or other information or 

comments, you may add it to the bottom of the data table in the diskette file or send it in hard 

copy. A printout of the data file format is shown in Table 2. 

Submit your results by December 15, 2005 as an attached file via e-mail to: 

E-mail: 

michele.schantz@nist.gov 

Further Information:

If you need further information, please contact Michele at the following address or phone 

numbers: 

Michele M. Schantz 

NIST 

100 Bureau Drive Stop 8392 

Gaithersburg, MD 20899-8392 

Phone: (301)975-3106 

FAX: (301)977-0685 
 

Table 1: Analytes of Interest in NIST Intercomparison Exercise Program for Organic 

Contaminants in the Marine Environment 

Chlorinated Pesticides 

hexachlorobenzene 2,4'-DDE 

alpha-HCH (alpha-BHC) 4,4'-DDE 

beta-HCH (beta-BHC) 2,4'-DDD 

gamma-HCH (gamma-BHC, Lindane) 4,4'-DDD 

heptachlor 2,4'-DDT 

heptachlor epoxide 4,4'-DDT 

cis-chlordane (alpha-chlordane) chlorpyrifos 

trans-chlordane (gamma-chlordane) aldrin 

oxychlordane dieldrin 

cis-nonachlor endrin 

trans-nonachlor endosulfan I 

mirex endosulfan II 

endosulfan sulfate 

Polychlorinated Biphenyl Congeners

 PCB No. Compound Name

 8 2,4'-dichlorobiphenyl 

 18 2,2',5-trichlorobiphenyl 

 28 2,4,4'-trichlorobiphenyl 

 31 2,4',5-trichlorobiphenyl 

 44 2,2',3,5'-tetrachlorobiphenyl 

 49 2,2',4,5'-tetrachlorobiphenyl 

 52 2,2',5,5'-tetrachlorobiphenyl 

 66 2,3',4,4'-tetrachlorobiphenyl 

 95 2,2',3,5',6-pentachlorobiphenyl 

 99 2,2',4,4',5-pentachlorobiphenyl 

 101 2,2',4,5,5'-pentachlorobiphenyl 

 105 2,3,3',4,4'-pentachlorobiphenyl 

 118 2,3',4,4',5-pentachlorobiphenyl 

 128 2,2',3,3',4,4'-hexachlorobiphenyl 

 138 2,2',3,4,4',5'-hexachlorobiphenyl 

 149 2,2',3,4',5',6-hexachlorobiphenyl 

 153 2,2',4,4',5,5'-hexachlorobiphenyl 

 156 2,3,3',4,4',5-hexachlorobiphenyl 

 170 2,2',3,3',4,4',5-heptachlorobiphenyl 

 180 2,2',3,4,4',5,5'-heptachlorobiphenyl 

 187 2,2',3,4',5,5',6-heptachlorobiphenyl 

 194 2,2',3,3',4,4',5,5'-octachlorobiphenyl 

 195 2,2',3,3',4,4',5,6-octachlorobiphenyl 

 206 2,2',3,3',4,4',5,5',6-nonachlorobiphenyl 

 209 decachlorobiphenyl 
Table 1. (continued) 

Polycyclic aromatic hydrocarbons (PAH)

naphthalene benz[a]anthracene 

2-methylnaphthalene chrysene 

1-methylnaphthalene triphenylene 

biphenyl benzo[b]fluoranthene 

2,6-dimethylnaphthalene benzo[j]fluoranthene 

acenaphthylene benzo[k]fluoranthene 

acenaphthene benzo[e]pyrene 

1,6,7-trimethylnaphthalene benzo[a]pyrene 

fluorene perylene 

phenanthrene indeno[1,2,3-cd]pyrene 

anthracene dibenz[a,h]anthracene 

1-methylphenanthrene benzo[ghi]perylene 

fluoranthene 

pyrene

Polybrominated diphenyl ethers (PBDEs)

 BDE 15 (4,4’-dibromo-) BDE 138 (2,2’,3,4,4’,5’-hexabromo-) 

 BDE 17 (2,2’,4-tribromo-) BDE 153 (2,2’,4,4’,5,5’-hexabromo-) 

 BDE 25 (2,3’,4-tribromo-) BDE 154 (2,2’,4,4’,5,6’-hexabromo-) 

 BDE 28 (2,4,4’-tribromo-) BDE 155 (2,2’,4,4’,6,6’-hexabromo-) 

 BDE 30 (2,4,6-tribromo-) BDE 156 (2,3,3’,4,4’,5-hexabromo-) 

 BDE 33 (2’,3,4-tribromo-) BDE 181 (2,2’,3,4,4’,5,6-heptabromo-) 

 BDE 47 (2,2’,4,4’-tetrabromo-) BDE 183 (2,2’,3,4,4’,5’,6-heptabromo-) 

 BDE 49 (2,2’,4,5’-tetrabromo-) BDE 190 (2,3,3’,4,4’,5,6-heptabromo-) 

 BDE 66 (2,3’,4,4’-tetrabromo-) BDE 191 (2,3,3’,4,4’,5,6’-heptabromo-) 

 BDE 71 (2,3’,4’,6-tetrabromo-) BDE 196 (2,2’,3,3’,4,4’,5,6’-octabromo-) 

 BDE 75 (2,4,4’,6-tetrabromo-) BDE 197 (2,2’,3,3’,4,4’,6,6’-octabbromo-) 

 BDE 85 (2,2’,3,4,4’-pentabromo-) BDE 203 (2,2’,3,4,4’,5,5’,6-octabromo-) 

 BDE 99 (2,2’,4,4’,5-pentabromo-) BDE 205 (2,3,3’,4,4’,5,5’,6-octabromo-) 

 BDE 100 (2,2’,4,4’,6-pentabromo-) BDE 206 (2,2’,3,3’,4,4’,5,6,6’-nonabromo-) 

 BDE 116 (2,3,4,5,6-pentabromo-) BDE 207 (2,2’3,3’,4,4’,5,6,6’-nonabromo-) 

 BDE 118 (2,3’,4,4’,5-pentabromo-) BDE 208 (2,2’,3,3’,4,5,5’,6,6’-nonabromo-) 

 BDE 119 (2,3’,4,4’,6-pentabromo-) BDE 209 (decabromo-)
Table 2. Diskette Data File Format (File: SED13.*)

NIST Intercomparison Exercise Program for Organics in the Marine Environment

NIST QA Program

Sample: QA05SED13 - Marine Sediment XIII

Please fill in all blanks; Use requested units of concentration; Report results as if 3 figures were significant

DO NOT INSERT ROWS OR COLUMNS WITHIN THIS TABLE. DO NOT MOVE CELLS.

 - If necessary, add additional data/information at the end of the table.

 - Use one of the following if no concentration is reported for an analyte:

 NA = Not analyzed/determined; <"conc" = <detection limit conc.; Other = other, explain in a note at end of table

 (DL = "below detection limit" may be used, but <"conc", e.g., <8, is preferable.)

 Do not use parentheses or negative numbers to indicate "less than detection limit".

Reporting Date (m/d/y): _______________________

Laboratory: _______________________

Submitted by: _______________________

BRIEF DESCRIPTION OF PROCEDURES USED:

Approximate amount of sample extracted: 

Sediment XIII ___________ g, wet basis; SRM 1941b ___________ g, dry basis

Method used for determining percentage water: ______________________________________________________________________

______________________________________________________________________

______________________________________________________________________

Were "wet" or "dry" samples extracted? 

Sediment XIII ___________ SRM 1941b ___________

Extraction method: ____________________________________________________________________________________________

Extraction solvent: ____________________________________________________________________________________________

Extraction time: ____________________________________________________________________________________________

Extraction - other: ____________________________________________________________________________________________

____________________________________________________________________________________________

Sample extract cleanup method: ____________________________________________________________________________________________

____________________________________________________________________________________________

____________________________________________________________________________________________

____________________________________________________________________________________________

____________________________________________________________________________________________

Analytical method used (e.g., GC-FID, GC-ECD):

Analyt. Instr. Column Phase Col. Length, m Col. i.d., mm Col. film thickness, μm

PAH ______________________________________________________________________

 Pesticides ______________________________________________________________________

 PCB Congeners ______________________________________________________________________

 BDE Congeners ______________________________________________________________________

Method of quantitation (IS = internal standard, ES = external standard):

 PAH __________________

 Pesticides __________________

 PCB Congeners __________________

 BDE Congeners __________________

IF internal standard method was used, please complete the following section:

 Identity of internal standards/surrogates used that were:

 Added PRIOR to extraction of sample:

 PAH ________________________________________________________________________

 Pesticides ________________________________________________________________________

 PCB Congeners ________________________________________________________________________

BDE Congeners ________________________________________________________________________

 Added after extraction/cleanup and JUST PRIOR to chromatographic analysis:

PAH ________________________________________________________________________

Pesticides ________________________________________________________________________

 PCB Congeners ________________________________________________________________________

BDE Congeners ________________________________________________________________________

 Any others? Added at what point in analyses__________________________________________________________

PAH ________________________________________________________________________

Pesticides ________________________________________________________________________

 PCB Congeners ________________________________________________________________________

BDE Congeners ________________________________________________________________________

 IS/surrogate standards used for quantitation calculations were: 

________ those added prior to extraction

________ those added after extraction/cleanup and just prior to chromatographic analysis

 If the IS/surrogates added after extraction/cleanup extraction were used for quantitation, 

 were results corrected for percent recovery? __________________
 Percent recovery range: PAH __________________

Pesticides __________________

PCB Congeners __________________

BDE Congeners __________________

Calibration Curve

Analytes outside of calibration 

Points Conc. Range curve calibration range

 PAH _______________________________ __________________________________

 Pesticides _______________________________ __________________________________

 PCB Congeners _______________________________ __________________________________

 BDE Congeners _______________________________ __________________________________

Were PCB congeners separated from pesticides prior to GC? __________________

Please note any differences in procedures used for SRM 1941b analyses from those for Marine Sediment XIII described above:

_____________________________________________________________________________________________________________________

_____________________________________________________________________________________________________________________

_____________________________________________________________________________________________________________________

_____________________________________________________________________________________________________________________

_____________________________________________________________________________________________________________________

RESULTS:

PERCENT WATER & total organic carbon, TOC (List each result if determined more than once. Enter results as a number, for example 90.0. 

DO NOT change format of cell to percent.)

Sediment XIII Sediment XIII Sediment XIII SRM 1941b SRM 1941b SRM 1941b

(percent) (percent) (percent) (percent) (percent) (percent)

Water __________ __________ __________ 

TOC __________ __________ __________ __________ __________ __________ 

PAH ANALYSES Sediment XIII Sediment XIII Sediment XIII SRM 1941b SRM 1941b SRM 1941b

Batch A Batch B Batch C Batch A Batch B Batch C

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

Analyst (Initials) __________ __________ __________ __________ __________ __________ 

Date(s) of measurements (m/d/y) __________ __________ __________ __________ __________ __________ 

Sample Jar number __________ __________ __________ 

Sediment XIII Sediment XIII Sediment XIII SRM 1941b SRM 1941b SRM 1941b

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

(ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass)

naphthalene __________ __________ __________ __________ __________ __________ 

2-methylnaphthalene __________ __________ __________ __________ __________ __________ 

1-methylnaphthalene __________ __________ __________ __________ __________ __________ 

biphenyl __________ __________ __________ __________ __________ __________ 

2,6-dimethylnaphthalene __________ __________ __________ __________ __________ __________ 

acenaphthylene __________ __________ __________ __________ __________ __________ 

acenaphthene __________ __________ __________ __________ __________ __________ 

1,6,7-trimethylnaphthalene __________ __________ __________ __________ __________ __________ 

fluorene __________ __________ __________ __________ __________ __________ 

phenanthrene __________ __________ __________ __________ __________ __________ 

anthracene __________ __________ __________ __________ __________ __________ 

1-methylphenanthrene __________ __________ __________ __________ __________ __________ 

fluoranthene __________ __________ __________ __________ __________ __________ 

pyrene __________ __________ __________ __________ __________ __________ 

benz[a]anthracene __________ __________ __________ __________ __________ __________ 

chrysene __________ __________ __________ __________ __________ __________ 

triphenylene __________ __________ __________ __________ __________ __________ 

benzo[b ]fluoranthene __________ __________ __________ __________ __________ __________ 

benzo[j ]fluoranthene __________ __________ __________ __________ __________ __________ 

benzo[k ]fluoranthene __________ __________ __________ __________ __________ __________ 

benzo[e]pyrene __________ __________ __________ __________ __________ __________ 

benzo[a]pyrene __________ __________ __________ __________ __________ __________ 

perylene __________ __________ __________ __________ __________ __________ 

indeno[1,2,3-cd]pyrene __________ __________ __________ __________ __________ __________ 

dibenz[a,h]anthracene __________ __________ __________ __________ __________ __________ 

benzo[ghi]perylene __________ __________ __________ __________ __________ __________ 
PESTICIDE ANALYSES Sediment XIII Sediment XIII Sediment XIII SRM 1941b SRM 1941b SRM 1941b

Batch A Batch B Batch C Batch A Batch B Batch C

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

Analyst (Initials) __________ __________ __________ __________ __________ __________ 

Date(s) of measurements (m/d/y) __________ __________ __________ __________ __________ __________ 

Sample Jar number __________ __________ __________ 

Sediment XIII Sediment XIII Sediment XIII SRM 1941b SRM 1941b SRM 1941b

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

(ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass)

alpha-HCH (a-BHC) __________ __________ __________ __________ __________ __________ 

hexachlorobenzene __________ __________ __________ __________ __________ __________ 

gamma-HCH (g-BHC,lindane) __________ __________ __________ __________ __________ __________ 

beta-HCH (b-BHC) __________ __________ __________ __________ __________ __________ 

heptachlor __________ __________ __________ __________ __________ __________ 

aldrin __________ __________ __________ __________ __________ __________ 

heptachlor epoxide __________ __________ __________ __________ __________ __________ 

oxychlordane __________ __________ __________ __________ __________ __________ 

gamma-chlordane __________ __________ __________ __________ __________ __________ 

2,4'-DDE __________ __________ __________ __________ __________ __________ 

endosulfan I __________ __________ __________ __________ __________ __________ 

cis-chlordane (alpha-chlordane) __________ __________ __________ __________ __________ __________ 

trans-nonachlor __________ __________ __________ __________ __________ __________ 

dieldrin __________ __________ __________ __________ __________ __________ 

4,4'-DDE __________ __________ __________ __________ __________ __________ 

2,4'-DDD __________ __________ __________ __________ __________ __________ 

endrin __________ __________ __________ __________ __________ __________ 

endosulfan II __________ __________ __________ __________ __________ __________ 

4,4'-DDD __________ __________ __________ __________ __________ __________ 

2,4'-DDT __________ __________ __________ __________ __________ __________ 

cis-nonachlor __________ __________ __________ __________ __________ __________ 

4,4'-DDT __________ __________ __________ __________ __________ __________ 

mirex __________ __________ __________ __________ __________ __________ 

endosulfan sulfate __________ __________ __________ __________ __________ __________ 

chlorpyrifos __________ __________ __________ __________ __________ __________ 

PCB CONGENER ANALYSES Sediment XIII Sediment XIII Sediment XIII SRM 1941b SRM 1941b SRM 1941b

Batch A Batch B Batch C Batch A Batch B Batch C

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

Analyst (Initials) __________ __________ __________ __________ __________ __________ 

Date(s) of measurements (m/d/y) __________ __________ __________ __________ __________ __________ 

Sample Jar number __________ __________ __________ 

Sediment XIII Sediment XIII Sediment XIII SRM 1941b SRM 1941b SRM 1941b

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

(ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass)

PCB 8 __________ __________ __________ __________ __________ __________ 

PCB 18 __________ __________ __________ __________ __________ __________ 

PCB 28 __________ __________ __________ __________ __________ __________ 

PCB 31 __________ __________ __________ __________ __________ __________ 

PCB 44 __________ __________ __________ __________ __________ __________ 

PCB 49 __________ __________ __________ __________ __________ __________ 

PCB 52 __________ __________ __________ __________ __________ __________ 

PCB 66 __________ __________ __________ __________ __________ __________ 

PCB 95 __________ __________ __________ __________ __________ __________ 

PCB 99 __________ __________ __________ __________ __________ __________ 

PCB 101 __________ __________ __________ __________ __________ __________ 

PCB 105 __________ __________ __________ __________ __________ __________ 

PCB 118 __________ __________ __________ __________ __________ __________ 

PCB 128 __________ __________ __________ __________ __________ __________ 

PCB 138 __________ __________ __________ __________ __________ __________ 

PCB 149 __________ __________ __________ __________ __________ __________ 

PCB 153 __________ __________ __________ __________ __________ __________ 

PCB 156 __________ __________ __________ __________ __________ __________ 

PCB 170 __________ __________ __________ __________ __________ __________ 

PCB 180 __________ __________ __________ __________ __________ __________ 

PCB 187 __________ __________ __________ __________ __________ __________ 

PCB 194 __________ __________ __________ __________ __________ __________ 

PCB 195 __________ __________ __________ __________ __________ __________ 

PCB 206 __________ __________ __________ __________ __________ __________ 

PCB 209 __________ __________ __________ __________ __________ __________ 
BDE CONGENER ANALYSES Sediment XIII Sediment XIII Sediment XIII SRM 1941b SRM 1941b SRM 1941b

Batch A Batch B Batch C Batch A Batch B Batch C

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

Analyst (Initials) __________ __________ __________ __________ __________ __________ 

Date(s) of measurements (m/d/y) __________ __________ __________ __________ __________ __________ 

Sample Jar number __________ __________ __________ 

Sediment XIII Sediment XIII Sediment XIII SRM 1941b SRM 1941b SRM 1941b

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

(ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass)

BDE 15 __________ __________ __________ __________ __________ __________ 

BDE 17 __________ __________ __________ __________ __________ __________ 

BDE 25 __________ __________ __________ __________ __________ __________ 

BDE 28 __________ __________ __________ __________ __________ __________ 

BDE 30 __________ __________ __________ __________ __________ __________ 

BDE 33 __________ __________ __________ __________ __________ __________ 

BDE 47 __________ __________ __________ __________ __________ __________ 

BDE 49 __________ __________ __________ __________ __________ __________ 

BDE 66 __________ __________ __________ __________ __________ __________ 

BDE 71 __________ __________ __________ __________ __________ __________ 

BDE 75 __________ __________ __________ __________ __________ __________ 

BDE 85 __________ __________ __________ __________ __________ __________ 

BDE 99 __________ __________ __________ __________ __________ __________ 

BDE 100 __________ __________ __________ __________ __________ __________ 

BDE 116 __________ __________ __________ __________ __________ __________ 

BDE 118 __________ __________ __________ __________ __________ __________ 

BDE 119 __________ __________ __________ __________ __________ __________ 

BDE 138 __________ __________ __________ __________ __________ __________ 

BDE 153 __________ __________ __________ __________ __________ __________ 

BDE 154 __________ __________ __________ __________ __________ __________ 

BDE 155 __________ __________ __________ __________ __________ __________ 

BDE 156 __________ __________ __________ __________ __________ __________ 

BDE 181 __________ __________ __________ __________ __________ __________ 

BDE 183 __________ __________ __________ __________ __________ __________ 

BDE 190 __________ __________ __________ __________ __________ __________ 

BDE 191 __________ __________ __________ __________ __________ __________ 

BDE 196 __________ __________ __________ __________ __________ __________ 

BDE 197 __________ __________ __________ __________ __________ __________ 

BDE 203 __________ __________ __________ __________ __________ __________ 

BDE 205 __________ __________ __________ __________ __________ __________ 

BDE 206 __________ __________ __________ __________ __________ __________ 

BDE 207 __________ __________ __________ __________ __________ __________ 

BDE 208 __________ __________ __________ __________ __________ __________ 

BDE 209 __________ __________ __________ __________ __________ __________ 

(Any additional data/information should be added here.)
Appendix C: Laboratory Notes Accompanying Data, 

Mussel Tissue XII 
Lab Additional notes for Mussel Tissue XII

1a Tissue XII Tissue XII Tissue XII SRM 2977 SRM 2977 SRM 2977

(percent) (percent) (percent) (percent) (percent) (percent)

% water 6.28 5.34 6.44 4.71 4.65 4.60

Tissue XII Tissue XII Tissue XII SRM 2977 SRM 2977 SRM 2977

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

(ng/g dry masng/g dry masng/g dry masng/g dry masng/g dry masnsg/g dry mass)

chrysene/triphenylene 131 124 129 88.7 84.4 87.6

dibenz[a,h + a,c ]anthracene 6.33 6.59 6.61 2.25 2.13 2.07

PCB 138/163 84.9 82.5 81.3 11.9 12.5 12.9

PCB 153/132 121 119 119 13.8 14.7 15.1

1c * BDEs determined by GC-MS NCI. 13C BDE was in sample as internal standard and interferes with determination of native.

4 Tissue XII Tissue XII Tissue XII SRM 2977 SRM 2977 SRM 2977

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

Percent water 8.0 7.8 7.8 12.8 13.0 12.6

Tissue XII Tissue XII Tissue XII SRM 2977 SRM 2977 SRM 2977

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

(ng/g dry masng/g dry masng/g dry masng/g dry masng/g dry masnsg/g dry mass)

dibenzothiophene 34.3 33.1 32.9 30.5 30.3 30.3

retene 10.5 10.3 10.5 3.88 3.30 4.02

nonachlor III <1.00 <0.397 <0.609 <0.690 <0.647 <0.618

PCB 17 8.66 7.94 8.37 2.15 2.23 1.85

PDB 33 5.95 6.21 6.27 1.77 1.64 1.74

PDB 70 62.0 60.2 59.6 3.57 3.40 3.56

PDB 74 31.7 30.6 30.9 2.73 2.62 2.62

PCB 82 10.4 9.94 10.0 0.667 0.542 0.586

PCB 87 39.9 37.9 37.8 1.89 1.56 1.82

PCB 110 81.7 80.0 79.3 6.65 6.24 6.52

PCB 151 15.8 15.8 15.5 3.17 3.16 3.15

PCB 158 9.47 9.10 9.15 <0.689 <0.646 <0.616

PCB 171 4.09 4.44 4.19 <0.692 <0.649 <0.620

PCB 177 9.71 9.87 9.72 1.95 1.97 2.00

PCB 183 11.3 11.2 11.1 0.703 0.678 0.700

PCB 191 <1.01 <0.400 <0.613 <0.695 <0.652 <0.622

PCB 199 <0.759 <0.300 <0.460 0.671 0.714 0.705

PCB 205 <1.01 <0.398 <0.611 <0.693 <0.650 <0.620

PCB 208 <1.01 <0.400 <0.613 <0.695 <0.652 <0.622

Notes: I - cannot be reported due to analytical interference which is not apparent in Tissue XII.

Chrysene includes triphenylene; BkF includes BjF; dibenz[a,h]anthracene includes dibenz[a,c]anthracene.

PCB 101 includes PCB 90; PCB 138 includes PCB 163 and PCB 164; PCB 153 includes PCB 132; 

PCB 187 includes PCB 159 and PCB 182.

5 INT = interference

7 1. For 3nd PT sample (Tissue XII), residue of Na2SO4 or water may be present in the extract.

2. PCB 101 and PCB 90 are coeluted.
8 All results are in wet weight as %moisture analysis was not performed on the tissue unknown sample or SRM.

Only a duplicate analysis was performed on the measurements for the unknown sample due to limited unknown sample volume for

 PAH, PCB and BDE analyses.

Only a duplicate analysis was performed on the SRM due to limited SRM volume for PAH, PCB and BDD analyses.

Reporting limit for BDE's are based on laboratory background levels and not calibration curve range.

PCB Coelutions:

PCB-8/PCB-5

PCB-43/PCB-49

PCB-52/PCB-73

PCB-66/PCB-80

PCB-89/PCB-90/PCB-101

PCB-93/PCB-95

PCB-105/PCB-127

PCB-106/PCB-118

PCB-138/PCB-163/PCB-164

PCB-139/PCB-149

PCB-170/PCB-190

PCB-182/PCB-187

BDE Coelutions:

BDE-17/BDE-25

BDE-28/BDE-33

BDE-119/BDE-120

BDE-198/BDE-203

9 The analyst notes that the following data are estimates due to chromatographic interferents: 

SRM 1 - PCB 180

Tissue XII Sample 1 - PCB 138

SRM 2 - PCB 138 & PCB 180

SRM 3 - PCB 180

10 PLEASE NOTE: Samples highlighted with color signify co-eluting congeners/compounds:

PCB : PCB 28+31

PAH : Chrysene + Triphenylene

PESTICIDE: 2,4' DDD + endrin

Only completed duplicate of SRM 2977

11 PCB Co-eluters: PCB-18/30, PCB-28/20/21/33, PCB-44/47/65, PCB-49/69, PCB-52/43/73, PCB-99/83, PCB-101/90/113, PCB-128/166

PCB Co-eluters: PCB-138/163/129/160, PCB-149/147, PCB-153/168, PCB-156/157, PCB-180/193

12 NA = not analyzed

"other" = congener co-elutes as follows:

PCB co-elutions: PCB 18/30, 20/28, 44/47/65, 95/100/93/102/98, 99/83, 101/90/113, 128/166, 138/163/129/160, 149/147, 153/168, 

156/157, 180/193

BDE co-elutions: BDE 17/25, 28/33, 119/120, 138/166

PAH co-elutions: triphenylene/chrysene, benzo[b]fluoranthene/benzo[j]fluoranthene, 

1,2,6-trimethynaphthalene/1,2,7-trimethynaphthalene/1,6,7-trimethylnaphthalene/2,3,5-trimethynaphthalene, 

dibenz[a,h]anthracene/dibenz[a,c]anthracene
Appendix D: Laboratory Notes Accompanying Data, Marine 

Sediment XIII 
Lab Additional notes for Sediment XIII

1a Sediment XIII Sediment XIII Sediment XIII SRM 1941b SRM 1941b SRM 1941b

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

(ng/g dry mass) (ng/g dry mass) (ng/g dry mass) (ng/g dry mass)(ng/g dry mass)(ng/g dry mass)

chrysene/triphenylene 409 406 416 445 441 417

dibenz[a,h + a,c]anthracene 87.3 86.8 87.4 84.7 87.5 86.3

PCB 138/163 4.89 4.89 4.96 4.91 4.74 4.73

PCB 153/132 6.56 6.41 5.53 6.27 6.53 6.56

1c * BDEs determined by GC-MS NCI. 13C BDE was in sample as internal standard and interferes with determination of native.

2 The SRM we used for the sediment analysis was SRM 1944.

The sample was extracted dry

About 0.3 g were extracted

RESULTS: (for SRM 1944)

PAH ANALYSES SRM 1944 SRM 1944 SRM 1944

Batch A Batch B Batch C

Sample 1 Sample 2 Sample 3

Analyst (Initials) JGL JGL JGL

Date(s) of measurements (m/d/y) 7/15/2005 7/22/2005 7/29/2005

(ng/g dry masng/g dry masng/g dry mass)

naphthalene 1620 1605 1510

2-methylnaphthalene 911 882 949

1-methylnaphthalene 586 568 571

biphenyl 257 259 271

2,6-dimethylnaphthalene 285 339 610

acenaphthylene 421 424 479

acenaphthene 490 494 486

1,6,7-trimethylnaphthalene NA NA NA

fluorene 987 963 937

phenanthrene 5690 5870 6060

anthracene 1550 1510 1640

1-methylphenanthrene 1740 1690 1720

fluoranthene 10300 10100 10000

pyrene 9810 10900 10400

benz[a]anthracene 5080 5170 5410

chrysene 5590 5580 5440

triphenylene NA NA NA

benzo[b ]fluoranthene 5400 6350 3750

benzo[j ]fluoranthene NA NA NA

benzo[k ]fluoranthene 2260 2080 2580

benzo[e]pyrene 3750 3420 3280

benzo[a]pyrene 4440 4560 3710

perylene 1110 1220 1110

indeno[1,2,3-cd]pyrene 2830 2510 3130

dibenz[a,h]anthracene 458 359 456

benzo[ghi]perylene 2930 2770 3240
4 Sediment XIII Sediment XIII Sediment XIII SRM 1941b

Batch A Batch B Batch C Batch A

Sample 1 Sample 2 Sample 3 Sample 1

Analyst (Initials) DarB DarB DarB DarB

Date(s) of measurements (m/d/y) 7/19/2005 7/19/2005 7/19/2005 7/19/2005

Sample Jar number 110 134 158

dibenzothiophene 59.9 57.2 58.9 60.2

retene 23.7 20.5 21.5 25.0

Sediment XIII Sediment XIII Sediment XIII SRM 1941b SRM 1941b SRM 1941b

Batch A Batch B Batch C Batch A Batch B Batch C

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

Analyst (Initials) RHB RHB RHB RHB RHB RHB

Date(s) of measurements (m/d/y) 12/5/2005 12/5/2005 12/5/2005 12/5/2005 12/5/2005 12/5/2005

Sample Jar number 110 134 158

nonachlor III <0.548 <0.479 <0.433 <0.474 <0.651 <0.505

PCB 17 1.31 1.34 1.31 1.37 1.19 1.36

PCB 33 2.39 2.37 2.48 2.50 2.22 2.78

PCB 70 5.77 5.66 5.80 5.69 5.17 5.56

PCB 74 2.23 2.20 2.22 2.29 2.08 2.21

PCB 82 0.551 0.515 0.547 0.520 0.479 0.567

PCB 87 1.74 1.59 1.90 1.55 1.45 1.57

PCB 110 6.72 6.48 7.11 6.51 5.88 6.40

PCB 151 1.26 1.48 1.28 1.20 1.07 1.20

PCB 158 <0.546 0.549 0.574 <0.472 <0.650 <0.504

PCB 171 <0.549 0.575 <0.434 <0.475 <0.653 <0.507

PCB 177 0.928 1.42 0.948 0.912 0.807 0.912

PCB 183 0.902 1.40 0.916 0.862 0.760 0.877

PCB 191 <0.551 <0.482 <0.436 <0.477 <0.656 <0.509

PCB 199 1.50 2.06 1.45 1.49 1.33 1.50

PCB 205 <0.549 <0.480 <0.434 <0.475 <0.654 <0.507

PCB 208 1.15 1.21 1.16 1.21 0.950 1.15

Notes: Chrysene includes triphenylene; BkF includes BjF; dibenz[a,h]anthracene includes dibenz[a,c]anthracene.

PCB 101 includes PCB 90; PCB 138 includes PCB 163 and PCB 164; PCB 153 includes PCB 132; PCB 187 includes PCB 159 and PCB 182.

7 1. PCB 101 and PCB 90 are coeluted.

2. The glassware containing the extract of the 2nd SRM sample was broken during operation and the sample was completely lost.

8 Only a duplicate analysis was performed on the measurements for the unknown sample due laboratory oversight for PCB 

and BDE analyses.

Only a duplicate analysis was performed on the SRM due to laboratory oversight for PAH, PCB and BDD analyses.

Reporting limit for BDE's are based on laboratory background levels and not calibration curve range.

BDE-209 could not be postively detected due to the loss of 13C-BDE-209 during analytical process possibly associated with matrix.

PCB Coelutions:

PCB-8/PCB-5

PCB-43/PCB-49

PCB-52/PCB-73

PCB-66/PCB-80

PCB-89/PCB-90/PCB-101

PCB-93/PCB-95

PCB-105/PCB-127

PCB-106/PCB-118

PCB-138/PCB-163/PCB-164

PCB-139/PCB-149

PCB-170/PCB-190

PCB-182/PCB-187

BDE Coelutions:

BDE-17/BDE-25

BDE-28/BDE-33

BDE-119/BDE-120

BDE-198/BDE-203
10 PLEASE NOTE: Samples highlighted with color signify co-eluting congeners/compounds:

PCB : PCB 28+31

PAH : Chrysene + Triphenylene

PESTICIDE: 2,4' DDD + endrin

Only completed duplicate of SRM 1941b, and trial 1 of Marine Sediment XIII was LOST 

due to evaporation at GCMS stage.

OTHER= LOST sample see note above

11 PCB Co-eluters: PCB-18/30, PCB-28/20/21/33, PCB-44/47/65, PCB-49/69, PCB-52/43/73, PCB-99/83, PCB-101/90/113, PCB-128/166, 

PCB Co-eluters: PCB-138/163/129/160, B93PCB-149/147, PCB-153/168, PCB-156/157, PCB-180/193

12 NA = not analyzed

"other" = congener co-elutes as follows:

PCB co-elutions: PCB 18/30, 20/28, 44/47/65, 95/100/93/102/98, 99/83, 101/90/113, 128/166, 138/163/129/160, 149/147, 153/168, 156/157, 

180/193

BDE co-elutions: BDE 17/25, 28/33, 119/120, 138/166

PAH co-elutions: triphenylene/chrysene, benzo[b]fluoranthene/benzo[j]fluoranthene, 

1,2,6-trimethynaphthalene/1,2,7-trimethynaphthalene/1,6,7-trimethylnaphthalene/2,3,5-trimethynaphthalene, 

dibenz[a,h]anthracene/dibenz[a,c]anthracene
Appendix E: Laboratory Methods Used, Mussel Tissue XII 
g extracted g extracted % TEO Extraction Extraction Extraction Extraction

Lab # Reported QA05TIS12 SRM 2977 Determination Method Solvent Time other

1a 11/28/2005 1 dry 3 dry

gravimetric using 100 μL 

of extract PFE dichloromethane 3 cycles each 5 min

temp = 100 oC; pressure 2000 psi; 3 

static cycles / sample

1c 2/22/2006 2 dry 2 dry

gravimetric using portion 

of extract PFE dichloromethane 3 cycles each 5 min

temp = 100 oC; pressure 2000 psi; 3 

static cycles / sample

3 12/15/2005 2 dry 2 dry not analyzed Sonication dichloromethane 3 x 2.0 min each4 1/4/2006 0.9 dry 0.9 dry gravimetric PFE dichloromethane approx. 16 min temp = 100 oC; pressure 2000 psi

5 1/10/2006 1.2 dry 1.2 dry

gravimetric using 1/6 of 

extract polytron dichloromethane (3 x 100 mL) 3 x 2.0 min each

filtered on glass fiber-filters (1.2 μm 

pore size) during extraction

6 1/13/2006 1 dry 1 dry

gravimetric using 1/10 of 

extract microscale extraction 3570 acetone: dichloromethane 24 h

solvent changes at specified time 

intervals

7 1/13/2006 1 dry 3 dry

gravimetric using 1/10 of 

extract Soxhlet EPA 3540 acetone:hexane (1:1, volume fraction) 24 h

8 1/16/2006 0.5 dry 1 dry Soxhlet

dichloromethane for PAHs; toluene for 

PCBs and PBDEs 16 h

9 1/17/2006 2 dry 2 dry

gravimetric using portion 

of extract PFE dichlorormethane

2 x 2 min high speed 

extractions followed by 30 min 

on shaker table

10 1/25/2006 1 dry 1 dry gravimetric Soxhlet dichloromethane 18 h11 2/6/2006 1.5 dry 1.5 dry

gravimetric using portion 

of extract PFE dichloromethane

12 2/6/2006 1 dry 1 dry gravimetric Soxhlet dichloromethane 16 h
PCBs and Pesticides Method of 

Lab # Sample extract cleanup method Separated? quantitation

1a

size exclusion chromatography (SEC); silica solid phase extraction (SPE) column; condition and 

elute with 15 mL of 10 % dichloromethane in hexane no IS

1c

1.8 g alumina column (5% deactivated) with 9 mL 35 % dichloromethane in hexane; 0.5 g 

aminopropyl SPE column using 10 mL of 10% dichloromethane in hexane yes IS

3 silica gel; activated copper; sulfuric acid no IS4

Gravity flow column with silica gel and neutral alumina, followed by HPLC-SEC to elute fraction 

containing analytes of interest no IS

5 SEC; fractionated on 7.4% deactivated silica gel yes IS6 SEC; silica gel cartridges no IS7 SEC for PAH, PCB, Pesticide, and PBDE; Florisil for PCB, Pesticide, and PBDE no IS8 silica gel only for PAH; silica gel and acid alumina for PCB and PBDE some IS9 alumina gravity column; HPLC-SEC fractionation no IS

10

alumina for PAHs; Florisil with petroleum ether for PCBs and with 1:1 dichloromethane:petroleum 

ether for pesticides yes IS

11 alumina added to PFE extraction cells prior to extraction; SEC; acid/base silica column yes IS/ES12

PAHs - SEC, silica; pesticides - SEC, Florisil; PCBs and PBDEs - SEC, Florisil, acid/base silica, 

alumina no IS
PAHs Calibration Curve

Lab # Instrument Phase Dimensions # points range

1a GC/MS HP-5MS 30m x 0.25 mm, 0.25μm film 5 5 ng - 1500 ng extracted1c GC/MS DB-XLB 30m x 0.18 mm, 0.18μm film 6 1.72 ng/g - 983 ng/g

3 GC/MS RTX-5 Sil MS 30m x 0.28 mm, 0.25μm film 5 5 ng/mL - 2000 ng/mL4 GC/MS DB-5 60m x 0.25 mm, 0.25μm film 7 0.011 ng/μL - 1.1 ng/μL5 GC/MS HP-5MS 30m x 0.25 mm, 0.25μm film 5 10 ng/mL - 500 ng/mL6 GC/MS RTX-5 60m x 0.25 mm, 0.25μm film 7 10 ppb - 10000 ppb7 GC/MS DB-XLB 60m x 0.25 mm, 0.25μm film 1 50 ppb8 GC/MS DB-5MS 30m x 0.25 mm, 0.25μm film 5 25 μg - 2500 μg9 GC/MS DB-5 60m x 0.25 mm, 0.25μm film 8 0.005 ng/μL - 10 ng/μL

10 GC/MS DB-5 30m x 0.25 mm, 0.25μm film 5 5 ng - 100 ng12 GC/MS DB-5 30m x 0.25 mm, 0.25μm film 5 50 ng/mL - 5000 ng/mL

PBDEs Calibration Curve

Lab # Instrument Phase Dimensions # points range

1c GC/MS NCI DB-XLB 30m x 0.18 mm, 0.18μm film 5 0.07 ng/g - 386 ng/g

4 GC/MS DB-5 60m x 0.25 mm, 0.25μm film 4 0.0025 ng/μL - 1 ng/μL7 GC/HRMS DB-5MS 30m x 0.25 mm, 0.25μm film 7 0.05 ppb - 100 ppb8 HRGC/MS DB-5HT 30m x 0.25 mm, 0.1μm film 5 20 pg - 500000 pg

12 GC/HRMS DB-5HT 30m x 0.25 mm, 0.1μm film 5 1 ng/mL - 2500 ng/mL
PCBs Calibration Curve PESTICIDES Calibration Curve

Lab # Instrument Phase Dimensions # points range Instrument Phase Dimensions # points range

1a GC/MS HP-5MS 30m x 0.25 mm, 0.25um 5 5 ng - 300 ng extracted GC/MS HP-5MS 30m x 0.25 mm, 0.25um 5 5 ng - 300 ng extracted1c GC/MS DB-XLB

30m x 0.18 mm, 0.18um 

film 6 0.29 ng/g - 3930 ng/g GC/MS DB-XLB

30m x 0.18 mm, 0.18um 

film 6 0.65 ng/g - 244 ng/g

3 GC/MS RTX-5

60m x 0.25 mm, 0.25um 

film 5 2 ng/mL - 100 ng/mL GC/MS RTX-5

60m x 0.25 mm, 0.25um 

film 5 2 ng/mL - 100 ng/mL

4 GC/MS DB-5

60m x 0.25 mm, 0.25um 

film 6

0.0012 ng/μL - 0.32 

ng/μL GC/MS DB-5

60m x 0.25 mm, 0.25um 

film 6

0.0012 ng/μL - 0.32 

ng/μL

5 GC-ECD

HP-5MS/ DBXLB 30m x 0.25 mm, 0.25um film 5 5 ng/mL - 50 ng/mL GC-ECD HP-5MS/ DB- XLB 30m x 0.25 mm, 0.25um film 5 5 ng/mL - 50 ng/mL

6 GC/MS RTX-5

60m x 0.25 mm, 0.25um 

film 8 0.25 ppb - 400 ppb GC-ECD RTX-5

60m x 0.25 mm, 0.25um 

film 7 0.2 ppb - 200 ppb

7 GC/MS DB-XLB

30m x 0.18 mm, 0.18um 

film 1 20 ppb GC/MS DB-XLB

30m x 0.18 mm, 0.18um 

film 1 20 ppb

8 HRGC/MS DB-5 60m x 0.32 mm, 0.25um 5 20 pg - 20000 pg9 GC-ECD DB-5

60m x 0.25 mm, 0.25um 

film 7 0.001 ng/μL - 1 ng/μL GC-ECD DB-5

60m x 0.25 mm, 0.25um 

film 7 0.001 ng/μL - 1 ng/μL

10 GC-ECD DB-5

60m x 0.25 mm, 0.25um 

film 5 1 ng - 43 ng GC-ECD DB-5

60m x 0.25 mm, 0.25um 

film 5 1 ng - 10 ng

11 GC/HRMS SPB-Octyl

30m x 0.25 mm, 0.25um 

film 6

0.2 ng/mL - 2000 

ng/mL

12 GC/HRMS SPB-Octyl 30m x 0.25 mm, 0.1um film 5 1 ng/mL - 2000 ng/mL GC/HRMS DB-5 60m x 0.25 mm, 0.1um film 5

10 ng/mL - 4000 

ng/mL
PAHs corrected for

Lab # IS/surrogate added prior to extraction Used? added prior to analysis Used? recovery? others?

1a

deuterated naphthalene, biphenyl, acenaphthene, phenanthrene, fluoranthene, pyrene, B[a]A, B[a]P, perylene, 

B[ghi]P, DB[a,h]A x

1c

deuterated naphthalene, biphenyl, acenaphthene, phenanthrene, fluoranthene, pyrene, B[a]A, B[a]P, perylene, 

B[ghi]P, DB[a,h]A x

3 deuterated napthalene, phenanthrene, and chrysene deuterated fluorene, acenaphthene, B[a]P x n

4 deuterated naphthalene, acenaphthene, B[a]P x hexamethylbenzene

deuterated phenanathrene 

prior to clean-up

5 deuterated naphthalene, acenaphthene, phenanthrene, fluoranthene, chrysene, B[a]P deuterated fluorene, pyrene, peryleen x n

6 deuterated 2-methyl naphthalene, pyrene, B[b]F deuterated naphthalene, acenphthene, phenanthrene, chrysene, perylene x n

7 17 deuterated PAHs x

8

deuterated naphthalene, acenaphthylene, acenaphthene, fluorene, phenathrene, pyrene, B[a]A, chrysene, B[b]F, 

B[k]F, B[a]P, perylene, I{1,2,3-cd]P, DB[a,h]A, B[ghi]P x deuterated 2-methylnaphthalene, anthracene, terphenyl, B[e]P

9 deuterated naphthalene, acenaphthene, phneanthrene, B[a]P deuterated acenaphthylene and chrysene x n

10 surrogates- deuterated naphthalene, acenaphthene, phenanthrene, chrysene, perylene IS- deuterated fluorene, anthracene, fluoranthene, B[a]P x

12

deuterated naphthalene, 2-methylnaphthalene, biphenyl, 2,6-dimethylnaphthalene, acenaphthylene, 

phenanthrene, fluoranthene, B[a]A, chrysene, B[b,k]F, B[a]P, perylene, DB[a,h]A, I[123-cd]P, B[ghi]P x deuterated acenaphthene, pyrene, B[e]P used to quantify labeled surrogates

PBDEs corrected for

Lab # IS/surrogate added prior to extraction Used? added prior to analysis Used? recovery? others?

1c 13C-PCB194,13C-t-chlodane,endosulfan-d4 x

4 PCB 103 x tetrachloro-o-xylene

tetrachloro-m-xylene prior to 

clean-up

7 13C-BDEs (3, 5, 28, 47, 99, 100, 118, 153, 183) x8 13C-BDEs (28, 47, 99, 100, 153, 154, 183, 209) x 13 C-PCBs (138, 202)

12 13C BDEs (15,28,47,77,99,100,126,153,154,183,209) x 13C PCBs (52, 138) used to quantify labeled surrogates

13C BDE 139 prior to cleanup
PCBs corrected for

Lab # IS/surrogate added prior to extraction Used? added prior to analysis Used? recovery? others?

1a PCB 103 and PCB 198 x

1c

13C-PCB 28, 52, 118, 153, 180, 194, 206; deuterated 4,4'-

DDE, 4,4'-DDD, 4,4'-DDT x

3

2',3,5-Trichlorobiphenyl, 2,2',4,6,6'-Pentachlorobiphenyl, 

2,3,3',4,5,5',6-Heptachlorobiphenyl

3-Chlorobiphenyl, 2,3,3',4,4',5,5',6-

Octachlorobiphenyl (13C12 labelled) x n

4 PCB 103 x tetrachloro-o-xylene

tetrachloro-m-xylene prior to 

clean-up

5 g-chlordene, PCB 103, PCB 198 IS - 4,4'-dibromooctafluorobiphenyl x n6 13C-PCB 19 and 202 13C-PCB 15 and 180 x n

7 246/246-HBB x 34/34-TBB

8

13C-PCB 3, 15, 28, 77, 81, 105, 114, 118, 123, 126, 156, 

157, 167, 169, 170, 180, 189, 194, 208, 209 x 13C-PCB 52, 101, 202 

9 Cl3(34); Cl6(152) CL5(96); Cl6(161) x n

10 Surrogates - PCB 14, 64, 166 IS - PCB 30 and 204 x11

13C-PCB 

1,3,4,19,15,54,104,37,155,81,77,123,118,188,114,105,126,2

02,156,157,169,208,189,205,206,209 x 13C-PCB 9,52,138, 194

13C-PCB 28, 111,178 prior to 

clean-up

12

13C-PCBs 

4,15,19,37,54,77,81,104,105,114,118,123,126,155,156,157,

167,169,170,180,188,189,202,205,206,208,209 x

13C-PCBs 9,52,101,138,194, used to 

quantify labelled surrogates only.

13C-PCBs 28,111,178, used as 

cleanup standards.
Pesticides corrected for

Lab # IS/surrogate added prior to extraction Used? added prior to analysis Used? recovery? others?

1a 13C- lindane, trans-nonachlor, 4,4'-DDE, 4,4'-DDT x

1c

13C-PCB 52, trans-chlordane, PCB 118, PCB 153; 

deuterated endosulfan I x

3 13C-gamma-BHC and 4,4'-DDT

3-Chlorobiphenyl, 2,3,3',4,4',5,5',6-

Octachlorobiphenyl (13C12 labelled) x n

4 PCB 103 x tetrachloro-o-xylene

tetrachloro-m-xylene prior 

to clean-up

5 g-chlordene, PCB 103, PCB 198 IS - 4,4'-dibromooctafluorobiphenyl x n6 TMX and DCB PCB 192 x n7 246/246-HBB x 34/34-TBB

89 Cl3(34); Cl6(152) CL5(96); Cl6(161) x n

10 IS - PCB 30 and 204 x

1112

13C-HCB, b-HCH, g-HCH, d-HCH, Heptachlor, Aldrin, 

Oxychlordane, t-Chlordane, t-Nonachlor, c-nonachlor, DDE, 

DDT, Mirex, Heptachlor-epoxide, Dieldrin, Endrin, 

Endosulfan-I, Endosulfan-II x

13C-PCBs 52, 138, 153, used to 

quantify labelled surrogates only.
Appendix F: Laboratory Methods Used, Marine Sediment XIII 
g extracted g extracted % water Extraction Extraction Extraction Extraction

Lab # Reported QA05SED13 SRM 1941b Determination Method Solvent Time other

1a 11/28/2005 9 wet 3 dry

freeze-dry until constant 

mass PFE dichloromethane 3 cycles each 5 min

temp = 100 oC; pressure 2000 psi; 3 

static cycles / sample

1c 2/22/2006 5 wet 5 dry freeze-drying PFE dichloromethane 3 cycles each 5 min

temp = 100 oC; pressure 2000 psi; 3 

static cycles / sample

2 12/14/2005 10 wet SRM 1944

gravimetric - oven 100 °C 

for 24 h PFE dichloromethane 10 min temp = 100 oC; pressure 2000 psi

3 12/15/2005 10 wet 5 dry standard method 2540G Sonication dichloromethane 3 x 2.0 min each

4 1/4/2006 1 wet 0.5 dry oven 120 °C overnight PFE dichloromethane approx. 16 min temp = 100 oC; pressure 2000 psi

6 1/13/2006 3 wet 3 dry assumed 100% microscale extraction 3570 acetone: dichloromethane 24 h

solvent changes at specified time 

intervals

7 1/13/2006 5 wet 5 dry oven 105 °C for 24 h Soxhlet EPA 3540 acetone:hexane (1:1, volume fraction) 24 h8 1/16/2006 1 -2 wet 0.5 -1 dry ASTM D2216-98 Soxhlet

dichloromethane for PAHs; toluene for 

PCBs and PBDEs 16 h

10 1/25/2006 1 wet 1 dry oven 100 °C for 24 h Soxhlet dichloromethane 18 h

11 2/6/2006 10 wet 1 dry oven overnight PFE dichloromethane12 2/6/2006 8.5 wet 5 dry gravimetric Soxhlet dichloromethane 16 h
PCBs and Pesticides Method of 

Lab # Sample extract cleanup method Separated? quantitation

1a

silica solid phase extraction (SPE) column; condition and elute with 15 mL of 10 % dichloromethane 

in hexane no IS

1c

Size exclusion chromatography (SEC); 1.8 g alumina column (5% deactivated) with 9 mL 35 % 

dichloromethane in hexane yes IS

2 silica gel, alumina, copper chromatography; SEC HPLC cleanup no IS3 silica gel; activated copper; sulfuric acid no IS4

Gravity flow column with silica gel and neutral alumina, followed by HPLC-SEC to elute fraction 

containing analytes of interest no IS

6 silica cartridge; PCB wer acid cleaned post silica no IS7 SEC for PAH, PCB, Pesticide, and PBDE; Florisil for PCB, Pesticide, and PBDE no IS8 silica gel only for PAH; silica gel and acid alumina for PCB and PBDE some IS9 alumina gravity column; HPLC-SEC fractionation no IS

10

alumina for PAHs; Florisil with petroleum ether for PCBs and with 1:1 dichloromethane:petroleum 

ether for pesticides yes IS

11 alumina added to PFE extraction cells prior to extraction; SEC; acid/base silica column yes IS/ES12 PAHs -silica; pesticides - Florisil; PCBs and PBDEs - Florisil, acid/base silica, alumina no IS
PAHs Calibration Curve

Lab # Instrument Phase Dimensions # points range

1a GC/MS HP-5MS 30m x 0.25 mm, 0.25μm film 5 5 ng - 1500 ng extracted1c GC/MS DB-XLB 30m x 0.18 mm, 0.18μm film 6 1.72 ng/g - 983 ng/g

2 GC/MS 5% phenyl 25m x 0.2 mm, 0.33μm film 5 6 ng/mL - 1800 ng/mL3 GC/MS RTX-5 Sil MS 30m x 0.28 mm, 0.25μm film 5 5 ng/mL - 2000 ng/mL4 GC/MS DB-5 60m x 0.25 mm, 0.25μm film 7 0.015 ng/μL - 10 ng/μL6 GC/MS RTX-5 60m x 0.25 mm, 0.25μm film 7 10 ppb - 10000 ppb7 GC/MS DB-XLB 60m x 0.25 mm, 0.25μm film 1 50 ppb8 GC/MS DB-5MS 30m x 0.25 mm, 0.25μm film 5 25 μg - 2500 μg

10 GC/MS DB-5 30m x 0.25 mm, 0.25μm film 5 5 ng - 100 ng12 GC/MS DB-5 30m x 0.25 mm, 0.25μm film 5 50 ng/mL - 5000 ng/mL

PBDEs Calibration Curve

Lab # Instrument Phase Dimensions # points range

1c GC/MS NCI DB-XLB 30m x 0.18 mm, 0.18μm film 5 0.07 ng/g - 386 ng/g

4 GC/MS DB-5 60m x 0.25 mm, 0.25μm film 4 0.0025 ng/μL - 1 ng/μL7 GC/HRMS DB-5MS 30m x 0.25 mm, 0.25μm film 7 0.05 ppb - 100 ppb8 HRGC/MS DB-5HT 30m x 0.25 mm, 0.1μm film 5 20 pg - 500000 pg

12 GC/HRMS DB-5HT 30m x 0.25 mm, 0.1μm film 5 1 ng/mL - 2500 ng/mL
PCBs Calibration Curve PESTICIDES Calibration Curve

Lab # Instrument Phase Dimensions # points range Instrument Phase Dimensions # points range

1a GC/MS HP-5MS 30m x 0.25 mm, 0.25um 5 5 ng - 300 ng extracted GC/MS HP-5MS 30m x 0.25 mm, 0.25um 5 5 ng - 300 ng extracted1c GC/MS DB-XLB

30m x 0.18 mm, 0.18um 

film 6 0.29 ng/g - 3930 ng/g GC/MS DB-XLB

30m x 0.18 mm, 0.18um 

film 6 0.65 ng/g - 244 ng/g

3 GC/MS RTX-5

60m x 0.25 mm, 0.25um 

film 5 2 ng/mL - 100 ng/mL GC/MS RTX-5

60m x 0.25 mm, 0.25um 

film 5 2 ng/mL - 100 ng/mL

4 GC/MS DB-5

60m x 0.25 mm, 0.25um 

film 6

0.0012 ng/μL - 0.32 

ng/μL GC/MS DB-5

60m x 0.25 mm, 0.25um 

film 6

0.0012 ng/μL - 0.32 

ng/μL

6 GC/MS RTX-5

60m x 0.25 mm, 0.25um 

film 8 0.25 ppb - 400 ppb GC-ECD RTX-5

60m x 0.25 mm, 0.25um 

film 7 0.2 ppb - 200 ppb

7 GC/MS DB-XLB

30m x 0.18 mm, 0.18um 

film 1 20 ppb GC/MS DB-XLB

30m x 0.18 mm, 0.18um 

film 1 20 ppb

8 HRGC/MS DB-5

60m x 0.32 mm, 0.25um 

film 5 20 pg - 20000 pg

10 GC-ECD DB-5

60m x 0.25 mm, 0.25um 

film 5 1 ng - 43 ng GC-ECD DB-5

60m x 0.25 mm, 0.25um 

film 5 1 ng - 10 ng

11 GC/HRMS SPB-Octyl

30m x 0.25 mm, 0.25um 

film 6

0.2 ng/mL - 2000 

ng/mL

12 GC/HRMS SPB-Octyl 30m x 0.25 mm, 0.1um film 5 1 ng/mL - 2000 ng/mL GC/HRMS DB-5 60m x 0.25 mm, 0.1um film 5

10 ng/mL - 4000 

ng/mL
PAHs corrected for

Lab # IS/surrogate added prior to extraction Used? added prior to analysis Used? recovery? others?

1a

deuterated naphthalene, biphenyl, acenaphthene, phenanthrene, fluoranthene, pyrene, B[a]A, B[a]P, perylene, 

B[ghi]P, DB[a,h]A x

1c

deuterated naphthalene, biphenyl, acenaphthene, phenanthrene, fluoranthene, pyrene, B[a]A, B[a]P, perylene, 

B[ghi]P, DB[a,h]A x

2 deuterated naphthalene, acenaphthene, phenanthrene, chrysene, B[a]P, perylene x HMB

3 deuterated napthalene, phenanthrene, and chrysene deuterated fluorene, acenaphthene, B[a]P x n

4 deuterated naphthalene, acenaphthene, B[a]P x hexamethylbenzene

deuterated phenanathrene 

prior to clean-up

6 deuterated 2-methyl naphthalene, pyrene, B[b]F x deuterated naphthalene, acenphthene, phenanthrene, chrysene, perylene n7 17 deuterated PAHs x8

deuterated naphthalene, acenaphthylene, acenaphthene, fluorene, phenathrene, pyrene, B[a]A, chrysene, B[b]F, 

B[k]F, B[a]P, perylene, I{1,2,3-cd]P, DB[a,h]A, B[ghi]P x deuterated 2-methylnaphthalene, anthracene, terphenyl, B[e]P

10 surrogates- deuterated naphthalene, acenaphthene, phenanthrene, chrysene, perylene IS- deuterated fluorene, anthracene, fluoranthene, B[a]P x

12

deuterated naphthalene, 2-methylnaphthalene, biphenyl, 2,6-dimethylnaphthalene, acenaphthylene, 

phenanthrene, fluoranthene, B[a]A, chrysene, B[b,k]F, B[a]P, perylene, DB[a,h]A, I[123-cd]P, B[ghi]P x deuterated acenaphthene, pyrene, B[e]P used to quantify labeled surrogates

PBDEs corrected for

Lab # IS/surrogate added prior to extraction Used? added prior to analysis Used? recovery? others?

1c 13C-PCB194,13C-t-chlodane,endosulfan-d4 x

4 PCB 103 x tetrachloro-o-xylene

tetrachloro-m-xylene prior to 

clean-up

7 13C-BDEs (3, 5, 28, 47, 99, 100, 118, 153, 183) x8 13C-BDEs (28, 47, 99, 100, 153, 154, 183, 209) x 13 C-PCBs (138, 202)

12 13C BDEs (15,28,47,77,99,100,126,153,154,183,209) x 13C PCBs (52, 138) used to quantify labeled surrogates

13C BDE 139 prior to cleanup
PCBs corrected for

Lab # IS/surrogate added prior to extraction Used? added prior to analysis Used? recovery? others?

1a PCB 103 and PCB 198 x

1c

13C-PCB 28, 52, 118, 153, 180, 194, 206; deuterated 4,4'-

DDE, 4,4'-DDD, 4,4'-DDT x

3

2',3,5-Trichlorobiphenyl, 2,2',4,6,6'-Pentachlorobiphenyl, 

2,3,3',4,5,5',6-Heptachlorobiphenyl

3-Chlorobiphenyl, 2,3,3',4,4',5,5',6-

Octachlorobiphenyl (13C12 labelled) x n

4 PCB 103 x tetrachloro-o-xylene

tetrachloro-m-xylene prior to 

clean-up

6 13C-PCB 19 and 202 x 13C-PCB 15 and 180 n

7 246/246-HBB x 34/34-TBB

8

13C-PCB 3, 15, 28, 77, 81, 105, 114, 118, 123, 126, 156, 

157, 167, 169, 170, 180, 189, 194, 208, 209 x 13C-PCB 52, 101, 202 

10 Surrogates - PCB 14, 64, 166 IS - PCB 30 and 204 x11

13C-PCB 

1,3,4,19,15,54,104,37,155,81,77,123,118,188,114,105,126,2

02,156,157,169,208,189,205,206,209 x 13C-PCB 9,52,138, 194

13C-PCB 28, 111,178 prior to 

clean-up

12

13C-PCBs 

4,15,19,37,54,77,81,104,105,114,118,123,126,155,156,157,

167,169,170,180,188,189,202,205,206,208,209 x

13C-PCBs 9,52,101,138,194, used to 

quantify labelled surrogates only.

13C-PCBs 28,111,178, used as 

cleanup standards.
Pesticides corrected for

Lab # IS/surrogate added prior to extraction Used? added prior to analysis Used? recovery? others?

1a 13C- lindane, trans-nonachlor, 4,4'-DDE, 4,4'-DDT x

1c

13C-PCB 52, trans-chlordane, PCB 118, PCB 153; 

deuterated endosulfan I x

3 13C-gamma-BHC and 4,4'-DDT

3-Chlorobiphenyl, 2,3,3',4,4',5,5',6-

Octachlorobiphenyl (13C12 labelled) x n

4 PCB 103 x tetrachloro-o-xylene

tetrachloro-m-xylene prior 

to clean-up

6 TMX and DCB x PCB 192 n7 246/246-HBB x 34/34-TBB8

10 IS - PCB 30 and 204 x

1112

13C-HCB, b-HCH, g-HCH, d-HCH, Heptachlor, Aldrin, 

Oxychlordane, t-Chlordane, t-Nonachlor, c-nonachlor, DDE, 

DDT, Mirex, Heptachlor-epoxide, Dieldrin, Endrin, 

Endosulfan-I, Endosulfan-II x

13C-PCBs 52, 138, 153, used to 

quantify labelled surrogates only.
Appendix G: Charts of Mussel Tissue XII and SRM 2977 

Results by Analyte 

See Tables 2 through 9 for results reported as <number, detection limit, etc. 

Charts for analytes with few reported numerical results are not included in this appendix. 

Note: The numbers added to the charts are the values reported that are off the scale of the chart. 

For Mussel Tissue XII plots: 

Solid line: exercise assigned value 

Dotted line: z = ± 1, i. e., 25 % from assigned value 

Dotted/dashed line: z = ± 2, i. e., 50 % from assigned value 

Dashed line: z = ± 3, i. e., 75 % from assigned value 

For SRM 2977 plots: 

Solid line: material certified concentration or target value (see caption of each plot) 

Dotted line: 95 % confidence interval (CI) 

Dashed line: 30 % from 95 % confidence interval (CI) 
0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

45.0

50.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

naphthalene Tissue XII (QA05TIS12)

Assigned value = 9.86 ng/g s = 6.07 ng/g 95% CL = 5.61 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

103

0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

45.0

50.0

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

naphthalene SRM 2977

Reference Value = 19 ± 5 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits

112
0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

2-methylnaphthalene Tissue XII (QA05TIS12)

Assigned value = 8.00 ng/g s = 5.16 ng/g 95% CL = 5.42 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 8

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

5.0

10.0

15.0

20.0

25.0

30.0

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

2-methylnaphthalene SRM 2977

Reference Value = 18 ± 5 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

1-methylnaphthalene Tissue XII (QA05TIS12)

Assigned value = 3.66 ng/g s = 2.44 ng/g 95% CL = 3.02 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 7

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

5.0

10.0

15.0

20.0

25.0

30.0

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

1-methylnaphthalene SRM 2977

Reference Value = 16 ± 5 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 7

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

4.50

5.00

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

biphenyl Tissue XII (QA05TIS12)

Assigned value = 1.91 ng/g s = 0.77 ng/g 95% CL = 0.95 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 7

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

38.0

0.0

1.0

2.0

3.0

4.0

5.0

6.0

7.0

8.0

9.0

10.0

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

biphenyl SRM 2977

Reference Value = 6.8 ± 0.6 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 7

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits

163
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

9.00

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

2,6-dimethylnaphthalene Tissue XII (QA05TIS12)

Assigned value = 4.67 ng/g s = 2.85 ng/g 95% CL = 4.54 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 4

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

18.0

20.0

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

2,6-dimethylnaphthalene SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

9.00

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

acenaphthylene Tissue XII (QA05TIS12)

Assigned value = 3.72 ng/g s = 2.40 ng/g 95% CL = 2.01 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 8

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

4.50

5.00

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

acenaphthylene SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 11 Quantitative Results: 6

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

acenaphthene Tissue XII (QA05TIS12)

Assigned value = 2.93 ng/g s = 2.21 ng/g 95% CL = 2.74 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 7

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

43.5

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

acenaphthene SRM 2977

Reference Value = 4.2 ± 0.4 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits

181
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

9.00

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

1,6,7-trimethylnaphthalene Tissue XII (QA05TIS12)

Assigned value = 4.78 ng/g s = 1.65 ng/g 95% CL = 2.62 ng/g (dry basis)

Reported Results: 4 Quantitative Results: 4

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

80.0

90.0

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

1,6,7-trimethylnaphthalene SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 5 Quantitative Results: 5

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

fluorene Tissue XII (QA05TIS12)

Assigned value = 3.64 ng/g s = 1.64 ng/g 95% CL = 1.52 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

5.0

10.0

15.0

20.0

25.0

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

fluorene SRM 2977

Certified Value = 10.24 ± 0.43 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 11

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0

20

40

60

80

100

120

140

160

180

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

phenanthrene Tissue XII (QA05TIS12)

Assigned value = 88.7 ng/g s = 17.9 ng/g 95% CL = 13.8 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 11

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

80.0

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

phenanthrene SRM 2977

Certified Value = 35.1 ± 3.8 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 11

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

2.0

4.0

6.0

8.0

10.0

12.0

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

anthracene Tissue XII (QA05TIS12)

Assigned value = 5.79 ng/g s = 3.24 ng/g 95% CL = 2.70 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

39.0

0.0

5.0

10.0

15.0

20.0

25.0

30.0

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

anthracene SRM 2977

Reference Value = 8 ± 4 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0

20

40

60

80

100

120

140

160

180

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

1-methylphenanthrene Tissue XII (QA05TIS12)

Assigned value = 89.7 ng/g s = 27.7 ng/g 95% CL = 19.8 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

1-methylphenanthrene SRM 2977

Reference Value = 44 ± 2 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0

50

100

150

200

250

300

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

fluoranthene Tissue XII (QA05TIS12)

Assigned value = 133 ng/g s = 30 ng/g 95% CL = 23 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 11

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0

20

40

60

80

100

120

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

fluoranthene SRM 2977

Certified Value = 38.7 ± 1.0 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 11

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0

50

100

150

200

250

300

350

400

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

pyrene Tissue XII (QA05TIS12)

Assigned value = 190 ng/g s = 40 ng/g 95% CL = 31 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 11

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0

20

40

60

80

100

120

140

160

180

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

pyrene SRM 2977

Certified Value = 78.9 ± 3.5 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 11

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

45.0

50.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

benz[a]anthracene Tissue XII (QA05TIS12)

Assigned value = 24.7 ng/g s = 7.1 ng/g 95% CL = 5.4 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

45.0

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

benz[a]anthracene SRM 2977

Certified Value = 20.3 ± 0.8 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0

20

40

60

80

100

120

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

chrysene Tissue XII (QA05TIS12)

Assigned value = 63.9 ng/g s = 22.4 ng/g 95% CL = 27.8 ng/g (dry basis)

Reported Results: 7 Quantitative Results: 7

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0

20

40

60

80

100

120

140

160

180

200

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

chrysene SRM 2977

Reference Value = 49 ± 2 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

80.0

90.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

benzo[b]fluoranthene Tissue XII (QA05TIS12)

Assigned value = 47.6 ng/g s = 12.6 ng/g 95% CL = 11.7 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

162

0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

45.0

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

benzo[b]fluoranthene SRM 2977

Certified Value = 11.01 ± 0.28 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits

88.7
0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

18.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

benzo[j]fluoranthene Tissue XII (QA05TIS12)

Assigned value = no target ng/g (dry basis)

Reported Results: 2 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

9.00

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

benzo[j]fluoranthene SRM 2977

Reference Value = 4.6 ± 0.2 ng/g (dry basis)

Reported Results: 2 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

45.0

50.0

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

benzo[k]fluoranthene Tissue XII (QA05TIS12)

Assigned value = 16.3 ng/g s = 2.4 ng/g 95% CL = 3.0 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

5.0

10.0

15.0

20.0

25.0

30.0

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

benzo[k]fluoranthene SRM 2977

Reference Value = 4 ± 1 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0

20

40

60

80

100

120

140

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

benzo[e]pyrene Tissue XII (QA05TIS12)

Assigned value = 74.7 ng/g s = 14.6 ng/g 95% CL = 10.5 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 11

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

166

0.0

5.0

10.0

15.0

20.0

25.0

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

benzo[e]pyrene SRM 2977

Certified Value = 13.1 ± 1.1 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits

61.6
0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

benzo[a]pyrene Tissue XII (QA05TIS12)

Assigned value = 7.25 ng/g s = 2.65 ng/g 95% CL = 2.04 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

144

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

benzo[a]pyrene SRM 2977

Certified Value = 8.35 ± 0.72 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits

37.9
0.0

1.0

2.0

3.0

4.0

5.0

6.0

7.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

perylene Tissue XII (QA05TIS12)

Assigned value = 3.51 ng/g s = 1.44 ng/g 95% CL = 2.30 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 6

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

17.1 26.2

0.00

1.00

2.00

3.00

4.00

5.00

6.00

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

perylene SRM 2977

Certified Value = 3.50 ± 0.76 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 6

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits

44.0
0.0

5.0

10.0

15.0

20.0

25.0

30.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

indeno[1,2,3-cd]pyrene Tissue XII (QA05TIS12)

Assigned value = 15.1 ng/g s = 6.4 ng/g 95% CL = 4.9 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

1.0

2.0

3.0

4.0

5.0

6.0

7.0

8.0

9.0

10.0

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

indeno[1,2,3-cd]pyrene SRM 2977

Certified Value = 4.84 ± 0.81 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

dibenz[a,h]anthracene Tissue XII (QA05TIS12)

Assigned value = no target ng/g (dry basis)

Reported Results: 7 Quantitative Results: 3

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

4.50

5.00

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

dibenz[a,h]anthracene SRM 2977

Certified Value = 1.41 ± 0.19 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 6

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

45.0

50.0

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

benzo[ghi]perylene Tissue XII (QA05TIS12)

Assigned value = 24.6 ng/g s = 6.4 ng/g 95% CL = 4.9 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 11

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

5.0

10.0

15.0

20.0

25.0

30.0

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

benzo[ghi]perylene SRM 2977

Certified Value = 9.53 ± 0.43 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1.80

2.00

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

alpha-HCH (a-BHC) Tissue XII (QA05TIS12)

Assigned value = no target ng/g (dry basis)

Reported Results: 9 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.050

0.100

0.150

0.200

0.250

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

alpha-HCH (a-BHC) SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 8 Quantitative Results: 1

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

hexachlorobenzene Tissue XII (QA05TIS12)

Assigned value = no target ng/g (dry basis)

Reported Results: 9 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1.80

2.00

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

hexachlorobenzene SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 8 Quantitative Results: 1

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

4.50

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

gamma-HCH (g-BHC,lindane) Tissue XII (QA05TIS12)

Assigned value = no target ng/g (dry basis)

Reported Results: 10 Quantitative Results: 3

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1.80

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

gamma-HCH (g-BHC,lindane) SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 9 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

0.50

1.00

1.50

2.00

2.50

3.00

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

beta-HCH (b-BHC) Tissue XII (QA05TIS12)

Assigned value = no target ng/g (dry basis)

Reported Results: 8 Quantitative Results: 3

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

2.0

4.0

6.0

8.0

10.0

12.0

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

beta-HCH (b-BHC) SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 7 Quantitative Results: 3

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

0.50

1.00

1.50

2.00

2.50

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

heptachlor Tissue XII (QA05TIS12)

Assigned value = no target ng/g (dry basis)

Reported Results: 10 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.20

0.40

0.60

0.80

1.00

1.20

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

heptachlor SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 9 Quantitative Results: 1

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1.80

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

aldrin Tissue XII (QA05TIS12)

Assigned value = no target ng/g (dry basis)

Reported Results: 10 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

0.80

0.90

1.00

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

aldrin SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 9 Quantitative Results: 1

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.000

0.100

0.200

0.300

0.400

0.500

0.600

0.700

0.800

0.900

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

heptachlor epoxide Tissue XII (QA05TIS12)

Assigned value = no target ng/g (dry basis)

Reported Results: 10 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.020

0.040

0.060

0.080

0.100

0.120

0.140

0.160

0.180

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

heptachlor epoxide SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 9 Quantitative Results: 1

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

4.50

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

oxychlordane Tissue XII (QA05TIS12)

Assigned value = no target ng/g (dry basis)

Reported Results: 9 Quantitative Results: 4

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

40.8

0.00

1.00

2.00

3.00

4.00

5.00

6.00

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

oxychlordane SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 8 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

gamma-chlordane Tissue XII (QA05TIS12)

Assigned value = 7.45 ng/g s = 1.00 ng/g 95% CL = 0.77 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

gamma-chlordane SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 9 Quantitative Results: 5

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

9.00

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

2,4'-DDE Tissue XII (QA05TIS12)

Assigned value = no target ng/g (dry basis)

Reported Results: 10 Quantitative Results: 3

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.20

0.40

0.60

0.80

1.00

1.20

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

2,4'-DDE SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 9 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.000

0.020

0.040

0.060

0.080

0.100

0.120

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

endosulfan I Tissue XII (QA05TIS12)

Assigned value = no target ng/g (dry basis)

Reported Results: 10 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

24.3

0.00

0.50

1.00

1.50

2.00

2.50

3.00

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

endosulfan I SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 9 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

5.0

10.0

15.0

20.0

25.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

cis-chlordane (alpha-chlordane) Tissue XII (QA05TIS12)

Assigned value = 12.1 ng/g s = 3.4 ng/g 95% CL = 2.8 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.50

1.00

1.50

2.00

2.50

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

cis-chlordane (alpha-chlordane) SRM 2977

Certified Value = 1.42 ± 0.13 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 6

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

18.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

trans-nonachlor Tissue XII (QA05TIS12)

Assigned value = 9.00 ng/g s = 1.24 ng/g 95% CL = 1.04 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

trans-nonachlor SRM 2977

Certified Value = 1.43 ± 0.10 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 6

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

dieldrin Tissue XII (QA05TIS12)

Assigned value = 6.70 ng/g s = 4.42 ng/g 95% CL = 4.64 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 7

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

9.00

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

dieldrin SRM 2977

Certified Value = 6.04 ± 0.52 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 6

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

4,4'-DDE Tissue XII (QA05TIS12)

Assigned value = 33.9 ng/g s = 7.6 ng/g 95% CL = 7.0 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

18.0

20.0

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

4,4'-DDE SRM 2977

Certified Value = 12.5 ± 1.6 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

2,4'-DDD Tissue XII (QA05TIS12)

Assigned value = 8.04 ng/g s = 2.86 ng/g 95% CL = 2.20 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

4.50

5.00

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

2,4'-DDD SRM 2977

Certified Value = 3.32 ± 0.29 ng/g (dry basis)

Reported Results: 7 Quantitative Results: 6

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

45.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

4,4'-DDD Tissue XII (QA05TIS12)

Assigned value = 21.7 ng/g s = 10.8 ng/g 95% CL = 10.0 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

4,4'-DDD SRM 2977

Certified Value = 4.30 ± 0.38 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

2,4'-DDT Tissue XII (QA05TIS12)

Assigned value = no target ng/g (dry basis)

Reported Results: 10 Quantitative Results: 3

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

2,4'-DDT SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 9 Quantitative Results: 1

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

cis-nonachlor Tissue XII (QA05TIS12)

Assigned value = 4.27 ng/g s = 0.76 ng/g 95% CL = 0.58 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.20

0.40

0.60

0.80

1.00

1.20

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

cis-nonachlor SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 8 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

4.50

5.00

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

4,4'-DDT Tissue XII (QA05TIS12)

Assigned value = 1.68 ng/g s = 0.63 ng/g 95% CL = 1.00 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 6

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

10.2

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

4,4'-DDT SRM 2977

Certified Value = 1.28 ± 0.18 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 3

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits

13.8
0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

4.50

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

mirex Tissue XII (QA05TIS12)

Assigned value = no target ng/g (dry basis)

Reported Results: 9 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.50

1.00

1.50

2.00

2.50

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

mirex SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 8 Quantitative Results: 1

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

PCB 8 Tissue XII (QA05TIS12)

Assigned value = 2.56 ng/g s = 0.60 ng/g 95% CL = 0.75 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

PCB 8 SRM 2977

Certified Value = 2.10 ± 0.15 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

2.0

4.0

6.0

8.0

10.0

12.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

PCB 18 Tissue XII (QA05TIS12)

Assigned value = 5.71 ng/g s = 1.87 ng/g 95% CL = 1.34 ng/g (dry basis)

Reported Results: 12 Quantitative Results: 12

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

PCB 18 SRM 2977

Certified Value = 2.65 ± 0.30 ng/g (dry basis)

Reported Results: 12 Quantitative Results: 11

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

45.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

PCB 28 Tissue XII (QA05TIS12)

Assigned value = 23.2 ng/g s = 4.6 ng/g 95% CL = 3.3 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 11

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

PCB 28 SRM 2977

Certified Value = 5.37 ± 0.44 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 11

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

PCB 31 Tissue XII (QA05TIS12)

Assigned value = 21.7 ng/g s = 3.1 ng/g 95% CL = 3.2 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

PCB 31 SRM 2977

Certified Value = 3.92 ± 0.24 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

10.0

20.0

30.0

40.0

50.0

60.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

PCB 44 Tissue XII (QA05TIS12)

Assigned value = 31.4 ng/g s = 11.2 ng/g 95% CL = 8.0 ng/g (dry basis)

Reported Results: 12 Quantitative Results: 12

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

PCB 44 SRM 2977

Certified Value = 3.25 ± 0.63 ng/g (dry basis)

Reported Results: 12 Quantitative Results: 11

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

PCB 49 Tissue XII (QA05TIS12)

Assigned value = 37.2 ng/g s = 11.3 ng/g 95% CL = 8.7 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

PCB 49 SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 10 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

80.0

90.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

PCB 52 Tissue XII (QA05TIS12)

Assigned value = 46.6 ng/g s = 10.7 ng/g 95% CL = 7.6 ng/g (dry basis)

Reported Results: 12 Quantitative Results: 12

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

2.0

4.0

6.0

8.0

10.0

12.0

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

PCB 52 SRM 2977

Certified Value = 8.37 ± 0.54 ng/g (dry basis)

Reported Results: 12 Quantitative Results: 12

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

80.0

90.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

PCB 66 Tissue XII (QA05TIS12)

Assigned value = 48.4 ng/g s = 12.4 ng/g 95% CL = 8.3 ng/g (dry basis)

Reported Results: 12 Quantitative Results: 12

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

PCB 66 SRM 2977

Certified Value = 3.64 ± 0.32 ng/g (dry basis)

Reported Results: 12 Quantitative Results: 12

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0

10

20

30

40

50

60

70

80

90

100

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

PCB 95 Tissue XII (QA05TIS12)

Assigned value = 51.5 ng/g s = 9.2 ng/g 95% CL = 8.5 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

PCB 95 SRM 2977

Certified Value = 5.39 ± 0.59 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

80.0

90.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

PCB 99 Tissue XII (QA05TIS12)

Assigned value = 47.0 ng/g s = 7.2 ng/g 95% CL = 6.7 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

PCB 99 SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0

20

40

60

80

100

120

140

160

180

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

PCB 101 Tissue XII (QA05TIS12)

Assigned value = 88.1 ng/g s = 14.4 ng/g 95% CL = 11.1 ng/g (dry basis)

Reported Results: 12 Quantitative Results: 12

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

18.0

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

PCB 101 SRM 2977

Certified Value = 11.2 ± 1.2 ng/g (dry basis)

Reported Results: 12 Quantitative Results: 11

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

10.0

20.0

30.0

40.0

50.0

60.0

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

PCB 105 Tissue XII (QA05TIS12)

Assigned value = 31.1 ng/g s = 5.3 ng/g 95% CL = 3.8 ng/g (dry basis)

Reported Results: 12 Quantitative Results: 12

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

PCB 105 SRM 2977

Certified Value = 3.76 ± 0.49 ng/g (dry basis)

Reported Results: 12 Quantitative Results: 12

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0

20

40

60

80

100

120

140

160

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

PCB 118 Tissue XII (QA05TIS12)

Assigned value = 79.9 ng/g s = 15.9 ng/g 95% CL = 11.4 ng/g (dry basis)

Reported Results: 12 Quantitative Results: 12

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

PCB 118 SRM 2977

Certified Value = 10.5 ± 1.0 ng/g (dry basis)

Reported Results: 12 Quantitative Results: 12

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

5.0

10.0

15.0

20.0

25.0

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

PCB 128 Tissue XII (QA05TIS12)

Assigned value = 13.0 ng/g s = 2.2 ng/g 95% CL = 1.7 ng/g (dry basis)

Reported Results: 12 Quantitative Results: 12

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

PCB 128 SRM 2977

Certified Value = 2.49 ± 0.28 ng/g (dry basis)

Reported Results: 12 Quantitative Results: 11

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0

20

40

60

80

100

120

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

PCB 138 Tissue XII (QA05TIS12)

Assigned value = 64.1 ng/g s = 14.9 ng/g 95% CL = 15.7 ng/g (dry basis)

Reported Results: 7 Quantitative Results: 7

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

18.0

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

PCB 138 SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 7 Quantitative Results: 7

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0

20

40

60

80

100

120

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

PCB 149 Tissue XII (QA05TIS12)

Assigned value = 61.5 ng/g s = 11.2 ng/g 95% CL = 11.7 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

PCB 149 SRM 2977

Certified Value = 9.23 ± 0.12 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0

20

40

60

80

100

120

140

160

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

PCB 153 Tissue XII (QA05TIS12)

Assigned value = 85.7 ng/g s = 29.6 ng/g 95% CL = 21.2 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 11

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

5.0

10.0

15.0

20.0

25.0

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

PCB 153 SRM 2977

Certified Value = 14.1 ± 1.0 ng/g (dry basis)

Reported Results: 11 Quantitative Results: 11

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

2.0

4.0

6.0

8.0

10.0

12.0

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

PCB 156 Tissue XII (QA05TIS12)

Assigned value = 5.64 ng/g s = 1.25 ng/g 95% CL = 1.16 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

PCB 156 SRM 2977

Certified Value = 0.960 ± 0.085 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

PCB 170 Tissue XII (QA05TIS12)

Assigned value = 1.80 ng/g s = 0.38 ng/g 95% CL = 0.29 ng/g (dry basis)

Reported Results: 12 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

4.50

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

PCB 170 SRM 2977

Certified Value = 2.95 ± 0.23 ng/g (dry basis)

Reported Results: 12 Quantitative Results: 11

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

18.0

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

PCB 180 Tissue XII (QA05TIS12)

Assigned value = 9.29 ng/g s = 3.11 ng/g 95% CL = 2.39 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

2.0

4.0

6.0

8.0

10.0

12.0

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

PCB 180 SRM 2977

Certified Value = 6.79 ± 0.67 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

PCB 187 Tissue XII (QA05TIS12)

Assigned value = 18.0 ng/g s = 4.0 ng/g 95% CL = 2.7 ng/g (dry basis)

Reported Results: 12 Quantitative Results: 12

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

PCB 187 SRM 2977

Certified Value = 4.76 ± 0.38 ng/g (dry basis)

Reported Results: 12 Quantitative Results: 12

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

0.80

0.90

1.00

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

PCB 194 Tissue XII (QA05TIS12)

Assigned value = 0.501 ng/g s = 0.084 ng/g 95% CL = 0.105 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 6

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

PCB 194 SRM 2977

Certified Value = 0.897 ± 0.042 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 7

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.000

0.100

0.200

0.300

0.400

0.500

0.600

0.700

0.800

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

PCB 195 Tissue XII (QA05TIS12)

Assigned value = no target ng/g (dry basis)

Reported Results: 12 Quantitative Results: 3

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.050

0.100

0.150

0.200

0.250

0.300

0.350

0.400

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

PCB 195 SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 12 Quantitative Results: 3

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.000

0.050

0.100

0.150

0.200

0.250

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

PCB 206 Tissue XII (QA05TIS12)

Assigned value = no target ng/g (dry basis)

Reported Results: 12 Quantitative Results: 3

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.010

0.020

0.030

0.040

0.050

0.060

0.070

0.080

0.090

0.100

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

PCB 206 SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 12 Quantitative Results: 3

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.000

0.020

0.040

0.060

0.080

0.100

0.120

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

PCB 209 Tissue XII (QA05TIS12)

Assigned value = no target ng/g (dry basis)

Reported Results: 12 Quantitative Results: 3

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.005

0.010

0.015

0.020

0.025

0.030

0.035

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

PCB 209 SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 12 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

BDE 17 Tissue XII (QA05TIS12)

Assigned value = 3.72 ng/g s = 0.66 ng/g 95% CL = 1.64 ng/g (dry basis)

Reported Results: 3 Quantitative Results: 3

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1.80

2.00

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

BDE 17 SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 3 Quantitative Results: 3

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

BDE 28 Tissue XII (QA05TIS12)

Assigned value = 3.08 ng/g s = 1.58 ng/g 95% CL = 1.97 ng/g (dry basis)

Reported Results: 5 Quantitative Results: 5

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

BDE 28 SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 5 Quantitative Results: 5

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

45.0

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

BDE 47 Tissue XII (QA05TIS12)

Assigned value = 23.3 ng/g s = 5.4 ng/g 95% CL = 6.7 ng/g (dry basis)

Reported Results: 5 Quantitative Results: 5

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

10.0

20.0

30.0

40.0

50.0

60.0

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

BDE 47 SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 5 Quantitative Results: 5

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

BDE 49 Tissue XII (QA05TIS12)

Assigned value = 6.75 ng/g s = 2.00 ng/g 95% CL = 4.97 ng/g (dry basis)

Reported Results: 4 Quantitative Results: 3

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.50

1.00

1.50

2.00

2.50

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

BDE 49 SRM 2977

Target Value = 1.02 ± 0.03 ng/g (dry basis)

Reported Results: 4 Quantitative Results: 3

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1.80

2.00

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

BDE 66 Tissue XII (QA05TIS12)

Assigned value = 0.984 ng/g s = 0.403 ng/g 95% CL = 0.642 ng/g (dry basis)

Reported Results: 5 Quantitative Results: 4

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.100

0.200

0.300

0.400

0.500

0.600

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

BDE 66 SRM 2977

Target Value = 0.375 ± 0.062 ng/g (dry basis)

Reported Results: 5 Quantitative Results: 3

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.000

0.100

0.200

0.300

0.400

0.500

0.600

0.700

0.800

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

BDE 85 Tissue XII (QA05TIS12)

Assigned value = 0.418 ng/g s = 0.074 ng/g 95% CL = 0.185 ng/g (dry basis)

Reported Results: 5 Quantitative Results: 3

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.050

0.100

0.150

0.200

0.250

0.300

0.350

0.400

0.450

0.500

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

BDE 85 SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 5 Quantitative Results: 3

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

5.0

10.0

15.0

20.0

25.0

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

BDE 99 Tissue XII (QA05TIS12)

Assigned value = 11.5 ng/g s = 3.4 ng/g 95% CL = 5.3 ng/g (dry basis)

Reported Results: 4 Quantitative Results: 4

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

9.00

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

BDE 99 SRM 2977

Target Value = 4.11 ± 0.40 ng/g (dry basis)

Reported Results: 4 Quantitative Results: 4

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

BDE 100 Tissue XII (QA05TIS12)

Assigned value = 6.85 ng/g s = 1.93 ng/g 95% CL = 2.40 ng/g (dry basis)

Reported Results: 5 Quantitative Results: 5

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

BDE 100 SRM 2977

Target Value = 1.06 ± 0.18 ng/g (dry basis)

Reported Results: 5 Quantitative Results: 5

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.000

0.020

0.040

0.060

0.080

0.100

0.120

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

BDE 138 Tissue XII (QA05TIS12)

Assigned value = no target ng/g (dry basis)

Reported Results: 4 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.010

0.020

0.030

0.040

0.050

0.060

0.070

1a1c

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101112

laboratory number

concentration (ng/g dry basis)

BDE 138 SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 13 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

0.80

0.90

1.00

1a1c

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101112

laboratory number

concentration (ng/g, dry basis)

BDE 153 Tissue XII (QA05TIS12)

Assigned value = 0.515 ng/g s = 0.097 ng/g 95% CL = 0.155 ng/g (dry basis)

Reported Results: 5 Quantitative Results: 4

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.020

0.040

0.060

0.080

0.100

0.120

0.140

0.160

0.180

0.200

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

BDE 153 SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 5 Quantitative Results: 3

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

0.20

0.40

0.60

0.80

1.00

1.20

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

BDE 154 Tissue XII (QA05TIS12)

Assigned value = 0.550 ng/g s = 0.103 ng/g 95% CL = 0.164 ng/g (dry basis)

Reported Results: 5 Quantitative Results: 4

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.020

0.040

0.060

0.080

0.100

0.120

0.140

0.160

0.180

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

BDE 154 SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 5 Quantitative Results: 3

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1a1c

3456789

101112

laboratory number

concentration (ng/g, dry basis)

BDE 155 Tissue XII (QA05TIS12)

Assigned value = no target ng/g (dry basis)

Reported Results: 3 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed line: z=±2 

(50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.005

0.010

0.015

0.020

0.025

0.030

0.035

1a1c

3456789

101112

laboratory number

concentration (ng/g dry basis)

BDE 155 SRM 2977

Target Value = no target ng/g (dry basis)

Reported Results: 3 Quantitative Results: 1

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% from 95% 

confidence limits
Appendix H: Charts of Marine Sediment XIII and SRM 1941b 

Results by Analyte 

See Tables 10 through 17 for results reported as <number, detection limit, etc. 

Charts for analytes with few reported numerical results are not included in this appendix. 

Note: The numbers added to the charts are the values reported that are off the scale of the chart. 

For Marine Sediment XIII plots: 

Solid line: exercise assigned value 

Dotted line: z = ± 1, i. e., 25 % from assigned value 

Dotted/dashed line: z = ± 2, i. e., 50 % from assigned value 

Dashed line: z = ± 3, i. e., 75 % from assigned value 

For SRM 1941b plots: 

Solid line: material certified concentration or target value (see caption of each plot) 

Dotted line: 95 % confidence interval (CI) 

Dashed line: 30 % from 95 % confidence interval (CI)
0

200

400

600

800

1000

1200

1400

1600

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

naphthalene Sediment XIII (QA05SED13)

Assigned value = 785 ng/g s = 186 ng/g 95% CL = 172 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

200

400

600

800

1000

1200

1400

1600

1800

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

naphthalene SRM 1941b

Certified Value = 848 ± 95 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0

50

100

150

200

250

300

350

400

450

500

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

2-methylnaphthalene Sediment XIII (QA05SED13)

Assigned value = 219 ng/g s = 66 ng/g 95% CL = 61 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

50

100

150

200

250

300

350

400

450

500

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

2-methylnaphthalene SRM 1941b

Reference Value = 276 ± 53 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0

20

40

60

80

100

120

140

160

180

200

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

1-methylnaphthalene Sediment XIII (QA05SED13)

Assigned value = 98.2 ng/g s = 24.0 ng/g 95% CL = 20.0 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

20

40

60

80

100

120

140

160

180

200

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

1-methylnaphthalene SRM 1941b

Reference Value = 127 ± 14 ng/g (dry basis)

Reported Results: 7 Quantitative Results: 7

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0

20

40

60

80

100

120

140

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

biphenyl Sediment XIII (QA05SED13)

Assigned value = 65.8 ng/g s = 23.6 ng/g 95% CL = 21.8 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

20

40

60

80

100

120

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

biphenyl SRM 1941b

Reference Value = 74 ± 8 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0

20

40

60

80

100

120

140

160

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

2,6-dimethylnaphthalene Sediment XIII (QA05SED13)

Assigned value = 81.3 ng/g s = 43.6 ng/g 95% CL = 40.3 ng/g (dry basis)

Reported Results: 7 Quantitative Results: 7

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

20

40

60

80

100

120

140

160

180

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

2,6-dimethylnaphthalene SRM 1941b

Reference Value = 75.9 ± 4.5 ng/g (dry basis)

Reported Results: 6 Quantitative Results: 6

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

80.0

90.0

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

acenaphthylene Sediment XIII (QA05SED13)

Assigned value = 45.1 ng/g s = 13.8 ng/g 95% CL = 11.5 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

20

40

60

80

100

120

140

160

180

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

acenaphthylene SRM 1941b

Reference Value = 53.3 ± 6.4 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.0

10.0

20.0

30.0

40.0

50.0

60.0

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

1,6,7-trimethylnaphthalene Sediment XIII (QA05SED13)

Assigned value = no target ng/g (dry basis)

Reported Results: 3 Quantitative Results: 3

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

80.0

90.0

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

1,6,7-trimethylnaphthalene SRM 1941b

Reference Value = 25.5 ± 5.1 ng/g (dry basis)

Reported Results: 4 Quantitative Results: 4

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0

20

40

60

80

100

120

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

fluorene Sediment XIII (QA05SED13)

Assigned value = 56.1 ng/g s = 12.2 ng/g 95% CL = 10.2 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

50

100

150

200

250

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

fluorene SRM 1941b

Certified Value = 85.0 ± 15.0 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0

100

200

300

400

500

600

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

phenanthrene Sediment XIII (QA05SED13)

Assigned value = 306 ng/g s = 89 ng/g 95% CL = 75 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

100

200

300

400

500

600

700

800

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

phenanthrene SRM 1941b

Certified Value = 406 ± 44 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0

50

100

150

200

250

300

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

anthracene Sediment XIII (QA05SED13)

Assigned value = 137 ng/g s = 47 ng/g 95% CL = 40 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

50

100

150

200

250

300

350

400

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

anthracene SRM 1941b

Certified Value = 184 ± 18 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0

20

40

60

80

100

120

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

1-methylphenanthrene Sediment XIII (QA05SED13)

Assigned value = 55.4 ng/g s = 18.2 ng/g 95% CL = 19.1 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

20

40

60

80

100

120

140

160

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

1-methylphenanthrene SRM 1941b

Certified Value = 73.2 ± 5.9 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0

100

200

300

400

500

600

700

800

900

1000

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

fluoranthene Sediment XIII (QA05SED13)

Assigned value = 496 ng/g s = 140 ng/g 95% CL = 117 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

200

400

600

800

1000

1200

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

fluoranthene SRM 1941b

Certified Value = 651 ± 50 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0

100

200

300

400

500

600

700

800

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

pyrene Sediment XIII (QA05SED13)

Assigned value = 421 ng/g s = 142 ng/g 95% CL = 118 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

100

200

300

400

500

600

700

800

900

1000

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

pyrene SRM 1941b

Certified Value = 581 ± 39 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0

50

100

150

200

250

300

350

400

450

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

benz[a]anthracene Sediment XIII (QA05SED13)

Assigned value = 241 ng/g s = 80 ng/g 95% CL = 67 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

100

200

300

400

500

600

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

benz[a]anthracene SRM 1941b

Certified Value = 335 ± 25 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0

100

200

300

400

500

600

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

chrysene Sediment XIII (QA05SED13)

Assigned value = 219 ng/g s = 62 ng/g 95% CL = 65 ng/g (dry basis)

Reported Results: 6 Quantitative Results: 6

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

100

200

300

400

500

600

700

800

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

chrysene SRM 1941b

Certified Value = 291 ± 31 ng/g (dry basis)

Reported Results: 5 Quantitative Results: 5

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0

100

200

300

400

500

600

700

800

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

benzo[b]fluoranthene Sediment XIII (QA05SED13)

Assigned value = 413 ng/g s = 174 ng/g 95% CL = 183 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

100

200

300

400

500

600

700

800

900

1000

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

benzo[b]fluoranthene SRM 1941b

Certified Value = 453 ± 21 ng/g (dry basis)

Reported Results: 7 Quantitative Results: 7

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0

100

200

300

400

500

600

700

800

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

benzo[k]fluoranthene SRM 1941b

Certified Value = 225 ± 18 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits

0

50

100

150

200

250

300

350

400

450

500

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

benzo[k]fluoranthene Sediment XIII (QA05SED13)

Assigned value = 180 ng/g s = 56 ng/g 95% CL = 47 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)
0

100

200

300

400

500

600

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

benzo[e]pyrene Sediment XIII (QA05SED13)

Assigned value = 286 ng/g s = 97 ng/g 95% CL = 75 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

100

200

300

400

500

600

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

benzo[e]pyrene SRM 1941b

Certified Value = 325 ± 25 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0

100

200

300

400

500

600

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

benzo[a]pyrene Sediment XIII (QA05SED13)

Assigned value = 282 ng/g s = 120 ng/g 95% CL = 111 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

100

200

300

400

500

600

700

800

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

benzo[a]pyrene SRM 1941b

Certified Value = 358 ± 17 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0

100

200

300

400

500

600

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

perylene Sediment XIII (QA05SED13)

Assigned value = 311 ng/g s = 131 ng/g 95% CL = 138 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

100

200

300

400

500

600

700

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

perylene SRM 1941b

Certified Value = 397 ± 45 ng/g (dry basis)

Reported Results: 7 Quantitative Results: 7

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0

50

100

150

200

250

300

350

400

450

500

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

indeno[1,2,3-cd]pyrene Sediment XIII (QA05SED13)

Assigned value = 258 ng/g s = 101 ng/g 95% CL = 85 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

100

200

300

400

500

600

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

indeno[1,2,3-cd]pyrene SRM 1941b

Certified Value = 341 ± 57 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0

20

40

60

80

100

120

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

dibenz[a,h]anthracene Sediment XIII (QA05SED13)

Assigned value = 42 ng/g s = 21 ng/g 95% CL = 34 ng/g (dry basis)

Reported Results: 6 Quantitative Results: 6

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

20

40

60

80

100

120

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

dibenz[a,h]anthracene SRM 1941b

Certified Value = 53 ± 10 ng/g (dry basis)

Reported Results: 5 Quantitative Results: 5

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0

50

100

150

200

250

300

350

400

450

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

benzo[ghi]perylene Sediment XIII (QA05SED13)

Assigned value = 244 ng/g s = 96 ng/g 95% CL = 81 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

100

200

300

400

500

600

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

benzo[ghi]perylene SRM 1941b

Certified Value = 307 ± 45 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.000

0.050

0.100

0.150

0.200

0.250

0.300

0.350

0.400

0.450

0.500

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

alpha-HCH (a-BHC) SRM 1941b

Target Value = no target ng/g (dry basis)

Reported Results: 7 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits

0.000

0.100

0.200

0.300

0.400

0.500

0.600

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

alpha-HCH (a-BHC) Sediment XIII (QA05SED13)

Assigned value = no target ng/g (dry basis)

Reported Results: 7 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)
0.0

1.0

2.0

3.0

4.0

5.0

6.0

7.0

8.0

9.0

10.0

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

hexachlorobenzene Sediment XIII (QA05SED13)

Assigned value = 5.38 ng/g s = 1.90 ng/g 95% CL = 2.00 ng/g (dry basis)

Reported Results: 7 Quantitative Results: 7

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

2.0

4.0

6.0

8.0

10.0

12.0

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

hexachlorobenzene SRM 1941b

Certified Value = 5.83 ± 0.38 ng/g (dry basis)

Reported Results: 7 Quantitative Results: 7

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

0.80

0.90

1.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

gamma-HCH (g-BHC,lindane) Sediment XIII (QA05SED13)

Assigned value = no target ng/g (dry basis)

Reported Results: 8 Quantitative Results: 3

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

16.4

0.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

0.80

0.90

1.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

gamma-HCH (g-BHC,lindane) SRM 1941b

Target Value = no target ng/g (dry basis)

Reported Results: 7 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits

15.8
0.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

0.80

0.90

1.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

beta-HCH (b-BHC) Sediment XIII (QA05SED13)

Assigned value = no target ng/g (dry basis)

Reported Results: 7 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.050

0.100

0.150

0.200

0.250

0.300

0.350

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

beta-HCH (b-BHC) SRM 1941b

Target Value = no target ng/g (dry basis)

Reported Results: 7 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.000

0.050

0.100

0.150

0.200

0.250

0.300

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

aldrin Sediment XIII (QA05SED13)

Assigned value = no target ng/g (dry basis)

Reported Results: 8 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.020

0.040

0.060

0.080

0.100

0.120

0.140

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

aldrin SRM 1941b

Target Value = no target ng/g (dry basis)

Reported Results: 8 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.000

0.100

0.200

0.300

0.400

0.500

0.600

0.700

0.800

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

heptachlor epoxide Sediment XIII (QA05SED13)

Assigned value = no target ng/g (dry basis)

Reported Results: 8 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.050

0.100

0.150

0.200

0.250

0.300

0.350

0.400

0.450

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

heptachlor epoxide SRM 1941b

Target Value = no target ng/g (dry basis)

Reported Results: 8 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

4.50

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

oxychlordane Sediment XIII (QA05SED13)

Assigned value = no target ng/g (dry basis)

Reported Results: 8 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

4.50

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

oxychlordane SRM 1941b

Target Value = no target ng/g (dry basis)

Reported Results: 8 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

0.20

0.40

0.60

0.80

1.00

1.20

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

gamma-chlordane Sediment XIII (QA05SED13)

Assigned value = 0.572 ng/g s = 0.148 ng/g 95% CL = 0.155 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 6

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.100

0.200

0.300

0.400

0.500

0.600

0.700

0.800

0.900

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

gamma-chlordane SRM 1941b

Certified Value = 0.566 ± 0.093 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 7

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1.80

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

2,4'-DDE Sediment XIII (QA05SED13)

Assigned value = 0.380 ng/g s = 0.103 ng/g 95% CL = 0.256 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 4

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

2,4'-DDE SRM 1941b

Reference Value = 0.380 ± 0.120 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 5

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

0.50

1.00

1.50

2.00

2.50

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

cis-chlordane (alpha-chlordane) Sediment XIII (QA05SED13)

Assigned value = 0.482 ng/g s = 0.058 ng/g 95% CL = 0.072 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 6

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1.80

2.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

cis-chlordane (alpha-chlordane) SRM 1941b

Certified Value = 0.85 ± 0.11 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 7

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.000

0.100

0.200

0.300

0.400

0.500

0.600

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

trans-nonachlor Sediment XIII (QA05SED13)

Assigned value = 0.286 ng/g s = 0.089 ng/g 95% CL = 0.093 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 6

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.100

0.200

0.300

0.400

0.500

0.600

0.700

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

trans-nonachlor SRM 1941b

Certified Value = 0.438 ± 0.073 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 6

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.000

0.100

0.200

0.300

0.400

0.500

0.600

0.700

0.800

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

dieldrin Sediment XIII (QA05SED13)

Assigned value = 0.386 ng/g s = 0.034 ng/g 95% CL = 0.084 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 3

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.050

0.100

0.150

0.200

0.250

0.300

0.350

0.400

0.450

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

dieldrin SRM 1941b

Target Value = no target ng/g (dry basis)

Reported Results: 8 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

4,4'-DDE Sediment XIII (QA05SED13)

Assigned value = 3.44 ng/g s = 1.08 ng/g 95% CL = 1.00 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

4,4'-DDE SRM 1941b

Certified Value = 3.22 ± 0.28 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1.80

2.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

2,4'-DDD Sediment XIII (QA05SED13)

Assigned value = 0.927 ng/g s = 0.624 ng/g 95% CL = 0.992 ng/g (dry basis)

Reported Results: 7 Quantitative Results: 4

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1.80

2.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

2,4'-DDD SRM 1941b

Target Value = no target ng/g (dry basis)

Reported Results: 3 Quantitative Results: 3

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

4,4'-DDD Sediment XIII (QA05SED13)

Assigned value = 4.18 ng/g s = 1.42 ng/g 95% CL = 1.77 ng/g (dry basis)

Reported Results: 7 Quantitative Results: 7

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

4,4'-DDD SRM 1941b

Certified Value = 4.66 ± 0.46 ng/g (dry basis)

Reported Results: 7 Quantitative Results: 6

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

2,4'-DDT Sediment XIII (QA05SED13)

Assigned value = no target ng/g (dry basis)

Reported Results: 8 Quantitative Results: 3

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.20

0.40

0.60

0.80

1.00

1.20

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

2,4'-DDT SRM 1941b

Target Value = no target ng/g (dry basis)

Reported Results: 8 Quantitative Results: 3

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

4.50

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

cis-nonachlor Sediment XIII (QA05SED13)

Assigned value = 0.454 ng/g s = 0.493 ng/g 95% CL = 1.22 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 4

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.100

0.200

0.300

0.400

0.500

0.600

0.700

0.800

0.900

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

cis-nonachlor SRM 1941b

Certified Value = 0.378 ± 0.053 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 4

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

0.80

0.90

1.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

4,4'-DDT Sediment XIII (QA05SED13)

Assigned value = 0.537 ng/g s = 0.174 ng/g 95% CL = 0.433 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 4

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

5.95

0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1.80

2.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

4,4'-DDT SRM 1941b

Reference Value = 1.12 ± 0.42 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 3

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.000

0.050

0.100

0.150

0.200

0.250

0.300

0.350

0.400

0.450

0.500

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

mirex Sediment XIII (QA05SED13)

Assigned value = no target ng/g (dry basis)

Reported Results: 7 Quantitative Results: 3

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

4.63 1.28

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

4.50

5.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

mirex SRM 1941b

Target Value = no target ng/g (dry basis)

Reported Results: 7 Quantitative Results: 1

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

9.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 8 Sediment XIII (QA05SED13)

Assigned value = 1.36 ng/g s = 0.42 ng/g 95% CL = 0.39 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 8 SRM 1941b

Certified Value = 1.65 ± 0.19 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 18 Sediment XIII (QA05SED13)

Assigned value = 2.04 ng/g s = 0.48 ng/g 95% CL = 0.37 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 18 SRM 1941b

Certified Value = 2.39 ± 0.29 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 28 Sediment XIII (QA05SED13)

Assigned value = 3.79 ng/g s = 0.97 ng/g 95% CL = 0.81 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

9.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 28 SRM 1941b

Certified Value = 4.52 ± 0.57 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 31 Sediment XIII (QA05SED13)

Assigned value = 2.83 ng/g s = 0.94 ng/g 95% CL = 0.87 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 31 SRM 1941b

Certified Value = 3.18 ± 0.41 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 44 Sediment XIII (QA05SED13)

Assigned value = 3.47 ng/g s = 0.99 ng/g 95% CL = 0.76 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 44 SRM 1941b

Certified Value = 3.85 ± 0.20 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 49 Sediment XIII (QA05SED13)

Assigned value = 3.64 ng/g s = 1.22 ng/g 95% CL = 1.02 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

9.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 49 SRM 1941b

Certified Value = 4.34 ± 0.28 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

9.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 52 Sediment XIII (QA05SED13)

Assigned value = 4.48 ng/g s = 1.39 ng/g 95% CL = 1.07 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

9.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 52 SRM 1941b

Certified Value = 5.24 ± 0.28 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.0

1.0

2.0

3.0

4.0

5.0

6.0

7.0

8.0

9.0

10.0

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 66 Sediment XIII (QA05SED13)

Assigned value = 4.32 ng/g s = 1.41 ng/g 95% CL = 1.18 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

9.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 66 SRM 1941b

Certified Value = 4.96 ± 0.53 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 95 Sediment XIII (QA05SED13)

Assigned value = 3.44 ng/g s = 1.25 ng/g 95% CL = 1.05 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 95 SRM 1941b

Certified Value = 3.93 ± 0.62 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

4.50

5.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 99 Sediment XIII (QA05SED13)

Assigned value = 2.45 ng/g s = 0.87 ng/g 95% CL = 0.73 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 99 SRM 1941b

Certified Value = 2.90 ± 0.36 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

9.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 101 Sediment XIII (QA05SED13)

Assigned value = 4.86 ng/g s = 1.36 ng/g 95% CL = 1.05 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 101 SRM 1941b

Certified Value = 5.11 ± 0.34 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

0.50

1.00

1.50

2.00

2.50

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 105 Sediment XIII (QA05SED13)

Assigned value = 1.26 ng/g s = 0.40 ng/g 95% CL = 0.31 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.50

1.00

1.50

2.00

2.50

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 105 SRM 1941b

Certified Value = 1.43 ± 0.10 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 118 Sediment XIII (QA05SED13)

Assigned value = 3.59 ng/g s = 1.26 ng/g 95% CL = 0.96 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 118 SRM 1941b

Certified Value = 4.23 ± 0.19 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 128 Sediment XIII (QA05SED13)

Assigned value = 0.684 ng/g s = 0.275 ng/g 95% CL = 0.197 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.20

0.40

0.60

0.80

1.00

1.20

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 128 SRM 1941b

Certified Value = 0.696 ± 0.044 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 138 Sediment XIII (QA05SED13)

Assigned value = 3.83 ng/g s = 1.23 ng/g 95% CL = 1.14 ng/g (dry basis)

Reported Results: 7 Quantitative Results: 7

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 138 SRM 1941b

Certified Value = 3.60 ± 0.28 ng/g (dry basis)

Reported Results: 7 Quantitative Results: 7

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 149 Sediment XIII (QA05SED13)

Assigned value = 4.18 ng/g s = 1.44 ng/g 95% CL = 1.20 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

9.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 149 SRM 1941b

Certified Value = 4.35 ± 0.26 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.0

1.0

2.0

3.0

4.0

5.0

6.0

7.0

8.0

9.0

10.0

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 153 Sediment XIII (QA05SED13)

Assigned value = 4.99 ng/g s = 2.46 ng/g 95% CL = 2.06 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 153 SRM 1941b

Certified Value = 5.47 ± 0.32 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.000

0.100

0.200

0.300

0.400

0.500

0.600

0.700

0.800

0.900

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 156 Sediment XIII (QA05SED13)

Assigned value = 0.444 ng/g s = 0.128 ng/g 95% CL = 0.107 ng/g (dry basis)

Reported Results: 8 Quantitative Results: 8

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.100

0.200

0.300

0.400

0.500

0.600

0.700

0.800

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 156 SRM 1941b

Certified Value = 0.507 ± 0.090 ng/g (dry basis)

Reported Results: 7 Quantitative Results: 7

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 170 Sediment XIII (QA05SED13)

Assigned value = 1.19 ng/g s = 0.36 ng/g 95% CL = 0.28 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.50

1.00

1.50

2.00

2.50

3.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 170 SRM 1941b

Certified Value = 1.35 ± 0.09 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 180 Sediment XIII (QA05SED13)

Assigned value = 2.97 ng/g s = 1.15 ng/g 95% CL = 0.83 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

4.50

5.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 180 SRM 1941b

Certified Value = 3.24 ± 0.51 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

4.50

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 187 Sediment XIII (QA05SED13)

Assigned value = 2.20 ng/g s = 0.69 ng/g 95% CL = 0.49 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 187 SRM 1941b

Certified Value = 2.17 ± 0.22 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1.80

2.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 194 Sediment XIII (QA05SED13)

Assigned value = 1.01 ng/g s = 0.50 ng/g 95% CL = 0.46 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 7

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1.80

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 194 SRM 1941b

Certified Value = 1.04 ± 0.06 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 8

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.000

0.100

0.200

0.300

0.400

0.500

0.600

0.700

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 195 Sediment XIII (QA05SED13)

Assigned value = 0.291 ng/g s = 0.166 ng/g 95% CL = 0.174 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 7

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

4.49

0.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

0.80

0.90

1.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 195 SRM 1941b

Certified Value = 0.645 ± 0.060 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 5

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits

3.83
0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

4.50

5.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 206 Sediment XIII (QA05SED13)

Assigned value = 1.91 ng/g s = 0.66 ng/g 95% CL = 0.55 ng/g (dry basis)

Reported Results: 10 Quantitative Results: 10

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 206 SRM 1941b

Certified Value = 2.42 ± 0.19 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

PCB 209 Sediment XIII (QA05SED13)

Assigned value = 4.02 ng/g s = 1.47 ng/g 95% CL = 1.36 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

1.0

2.0

3.0

4.0

5.0

6.0

7.0

8.0

9.0

10.0

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

PCB 209 SRM 1941b

Certified Value = 4.86 ± 0.45 ng/g (dry basis)

Reported Results: 9 Quantitative Results: 9

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.000

0.020

0.040

0.060

0.080

0.100

0.120

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

BDE 28 Sediment XIII (QA05SED13)

Assigned value = 0.056 ng/g s = 0.005 ng/g 95% CL = 0.042 ng/g (dry basis)

Reported Results: 5 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.050

0.100

0.150

0.200

0.250

0.300

0.350

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

BDE 28 SRM 1941b

Target Value = 0.18 ± 0.07 ng/g (dry basis)

Reported Results: 5 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1.80

2.00

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

BDE 47 Sediment XIII (QA05SED13)

Assigned value = 1.03 ng/g s = 0.35 ng/g 95% CL = 0.86 ng/g (dry basis)

Reported Results: 5 Quantitative Results: 3

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.50

1.00

1.50

2.00

2.50

3.00

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

BDE 47 SRM 1941b

Target Value = 1.48 ± 0.51 ng/g (dry basis)

Reported Results: 5 Quantitative Results: 3

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.000

0.010

0.020

0.030

0.040

0.050

0.060

0.070

0.080

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

BDE 66 Sediment XIII (QA05SED13)

Assigned value = 0.038 ng/g s = 0.009 ng/g 95% CL = 0.078 ng/g (dry basis)

Reported Results: 5 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.005

0.010

0.015

0.020

0.025

0.030

0.035

0.040

0.045

0.050

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

BDE 66 SRM 1941b

Target Value = no target ng/g (dry basis)

Reported Results: 5 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.000

0.010

0.020

0.030

0.040

0.050

0.060

0.070

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

BDE 85 Sediment XIII (QA05SED13)

Assigned value = no target ng/g (dry basis)

Reported Results: 5 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.010

0.020

0.030

0.040

0.050

0.060

0.070

0.080

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

BDE 85 SRM 1941b

Target Value = no target ng/g (dry basis)

Reported Results: 5 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

BDE 99 Sediment XIII (QA05SED13)

Assigned value = no target ng/g (dry basis)

Reported Results: 5 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1.80

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

BDE 99 SRM 1941b

Target Value = 0.62 ± 0.19 ng/g (dry basis)

Reported Results: 5 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.000

0.050

0.100

0.150

0.200

0.250

0.300

0.350

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

BDE 100 Sediment XIII (QA05SED13)

Assigned value = no target ng/g (dry basis)

Reported Results: 5 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.050

0.100

0.150

0.200

0.250

0.300

0.350

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

BDE 100 SRM 1941b

Target Value = 0.15 ± 0.06 ng/g (dry basis)

Reported Results: 5 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.000

0.005

0.010

0.015

0.020

0.025

0.030

0.035

0.040

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

BDE 138 Sediment XIII (QA05SED13)

Assigned value = no target ng/g (dry basis)

Reported Results: 4 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.005

0.010

0.015

0.020

0.025

0.030

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

BDE 138 SRM 1941b

Target Value = no target ng/g (dry basis)

Reported Results: 4 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.000

0.020

0.040

0.060

0.080

0.100

0.120

0.140

0.160

0.180

0.200

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

BDE 153 Sediment XIII (QA05SED13)

Assigned value = no target ng/g (dry basis)

Reported Results: 5 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.050

0.100

0.150

0.200

0.250

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

BDE 153 SRM 1941b

Target Value = 0.09 ± 0.04 ng/g (dry basis)

Reported Results: 5 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
0.000

0.020

0.040

0.060

0.080

0.100

0.120

0.140

0.160

1a1c

234678

101112

laboratory number

concentration (ng/g, dry basis)

BDE 154 Sediment XIII (QA05SED13)

Assigned value = no target ng/g (dry basis)

Reported Results: 5 Quantitative Results: 2

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.000

0.020

0.040

0.060

0.080

0.100

0.120

0.140

0.160

0.180

1a1c

234678

101112

laboratory number

concentration (ng/g dry basis)

BDE 154 SRM 1941b

Target Value = 0.09 ± 0.02 ng/g (dry basis)

Reported Results: 5 Quantitative Results: 2

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
Appendix I: Data Submitted After the First Draft of this Report 

Was Distributed to the Participants
 Two Laboratories Submitted Data after the first draft of this report for Mussel TissueXII (QA05TIS12) and the associated control sample.

QA05TIS12 QA05TIS12 QA05TIS12 SRM 2977

Laboratory No. Lab 14 Lab 15 Exercise Assigned Lab 14 Lab 15

mean RSD mean RSD Value s 95% CL mean RSD

TEO (percent) 5.7 6.5 3.46% 3.81 4.73 3.96 SRM 1974b 6.51 3.46%

Lab 14 Lab 15 Exercise Assigned Lab 14 Lab 15

PAHs (ng/g dry mass) mean RSD mean RSD Value s 95% CL mean RSD From 2977 Certificate of Analysis naphthalene 23.0 15.65% 18.1 8.93% 9.86 6.07 5.61 SRM 1974b 11.8 70.85% 19 5 Reference2-methylnaphthalene 13.9 7.10% 9.51 0.15% 8.00 5.16 5.42 SRM 1974b 9.37 52.15% 18 5 Reference1-methylnaphthalene 6.42 11.95% 5.70 3.45% 3.66 2.44 3.02 SRM 1974b 7.43 42.91% 16 5 Referencebiphenyl 6.71 14.00% 8.68 2.94% 1.91 0.77 0.95 SRM 1974b 5.78 69.96% 6.8 0.6 Reference2,6-dimethylnaphthalene 3.59 11.36% 4.07 3.96% 4.67 2.85 4.54 SRM 1974b 13.2 13.21% no target Targetacenaphthylene 5.09 5.51% 0.605 8.61% 3.72 2.40 2.01 SRM 1974b 2.20 16.22% no target Targetacenaphthene 1.11 6.18% 2.93 2.21 2.74 SRM 1974b 2.63 9.16% 4.2 0.4 Reference1,6,7-trimethylnaphthalene 7.21 15.51% 3.30 2.19% 4.78 1.65 2.62 SRM 1974b 16.9 0.90% no target Targetfluorene 4.84 11.39% 2.95 2.94% 3.64 1.64 1.52 SRM 1974b 6.87 3.04% 10.24 0.43 Certified

phenanthrene 25.8 8.10% 74.8 7.90% 88.7 17.9 13.8 SRM 1974b 31.3 0.99% 35.1 3.8 Certifiedanthracene 5.76 10.15% 0.981 13.03% 5.79 3.24 2.70 SRM 1974b 2.29 11.06% 8 4 Reference1-methylphenanthrene 10.4 25.88% 55.8 14.79% 89.7 27.7 19.8 SRM 1974b 44.6 10.42% 44 2 Referencefluoranthene 167 17.79% 126 2.10% 133 30 23 SRM 1974b 28.8 4.71% 38.7 1 Certifiedpyrene 198 4.51% 126 2.43% 190 40 31 SRM 1974b 58.1 11.54% 78.9 3.5 Certifiedbenz[a]anthracene 47.5 13.86% 27.3 12.41% 24.7 7.1 5.4 SRM 1974b 14.0 8.17% 20.34 0.78 Certifiedchrysene 90.0 11.54% 104 4.73% 63.9 22.4 27.8 SRM 1974b 69.0 4.60% 49 2 Referencetriphenylene NA no target SRM 1974b NA 39 1 Referencebenzo[b]fluoranthene 70.4 4.26% 87.8 7.21% 47.6 12.6 11.7 SRM 1974b 17.8 6.33% 11.01 0.28 Certifiedbenzo[j]fluoranthene 52.5 3.36% NA no target SRM 1974b NA 4.6 0.2 Referencebenzo[k]fluoranthene 16.6 9.71% 16.3 2.4 3.0 SRM 1974b 5.39 12.47% 4 1 Referencebenzo[e]pyrene 95.3 1.82% 124 2.32% 74.7 14.6 10.5 SRM 1974b 17.4 6.15% 13.1 1.1 Certifiedbenzo[a]pyrene 28.9 25.18% 4.28 1.19% 7.25 2.65 2.04 SRM 1974b 5.08 5.94% 8.35 0.72 Certifiedperylene 10.3 3.95% 3.08 10.77% 3.51 1.44 2.30 SRM 1974b 2.70 26.78% 3.5 0.76 Certifiedindeno[1,2,3-cd]pyrene 24.1 8.27% 13.3 3.25% 15.1 6.4 4.9 SRM 1974b 2.78 14.08% 4.84 0.81 Certifieddibenz[a,h]anthracene 4.22 5.63% no target SRM 1974b 2.19 82.20% 1.41 0.19 Certifiedbenzo[ghi]perylene 33.2 12.18% 26.5 4.46% 24.6 6.4 4.9 SRM 1974b 8.17 11.64% 9.53 0.43 Certified
QA05TIS12 QA05TIS12 QA05TIS12 SRM 2977

Pesticides Lab 14 Lab 15 Exercise Assigned Lab 14 Lab 15 (ng/g dry mass) mean RSD mean RSD Value s 95% CL mean RSD From 2977 Certificate of Analysis alpha-HCH (a-BHC) NA <0.69 no target SRM 1974b <0.69 no targethexachlorobenzene NA 0.093 50.10% no target SRM 1974b 0.266 28.08% no targetgamma-HCH (g-BHC,lindane) NA <0.66 no target SRM 1974b <0.66 no target Targetbeta-HCH (b-BHC) NA <0.68 no target SRM 1974b 7.49 7.70% no target Targetheptachlor NA <0.75 no target SRM 1974b <0.75 no target Targetaldrin NA <0.73 no target SRM 1974b <0.73 no target Targetheptachlor epoxide NA <0.68 no target SRM 1974b <0.68 no target Targetoxychlordane NA <0.83 no target SRM 1974b <0.83 no target Targetgamma-chlordane NA 6.13 12.15% 7.45 1.00 0.77 SRM 1974b 0.870 3.93% no target Target2,4'-DDE 7.89 13.43% 1.84 2.44% no target SRM 1974b 0.264 7.09% no target Targetendosulfan I NA <0.74 no target SRM 1974b <0.74 no target Targetcis-chlordane (alpha-chlordane) NA 8.42 5.58% 12.1 3.4 2.8 SRM 1974b 0.588 9.93% 1.42 0.13 Targettrans-nonachlor NA 8.95 4.10% 9.00 1.24 1.04 SRM 1974b 1.04 10.11% 1.43 0.1 Targetdieldrin NA 2.55 23.53% 6.70 4.42 4.64 SRM 1974b 5.33 3.71% 6.04 0.52 Certified4,4'-DDE 38.9 2.80% 33.3 1.22% 33.9 7.6 7.0 SRM 1974b 8.87 3.97% 12.5 1.6 Certified2,4'-DDD 7.33 12.68% 10.7 7.35% 8.04 2.86 2.20 SRM 1974b 2.96 3.91% 3.32 0.29 Certified

endrin NA <0.61 no target SRM 1974b 1.22 11.79% no target Certifiedendosulfan II NA <0.74 no target SRM 1974b <0.74 no target Certified4,4'-DDD 21.6 4.75% 21.9 5.87% 21.7 10.8 10.0 SRM 1974b 3.04 14.56% 4.3 0.38 Target2,4'-DDT <2.98 4.31 0.58% no target SRM 1974b 1.44 38.58% no target Targetcis-nonachlor NA 6.03 2.07% 4.27 0.76 0.58 SRM 1974b 1.91 5.46% no target Certified4,4'-DDT 1.35 24.79% 2.55 36.81% 1.68 0.63 1.00 SRM 1974b 0.897 8.17% 1.28 0.18 Targetmirex NA 0.345 2.68% no target SRM 1974b 0.363 43.50% no target Targetendosulfan sulfate NA <0.81 no target SRM 1974b <0.81 no target Certifiedchlorpyrifos NA <0.74 no target SRM 1974b <0.74 no target Target
QA05TIS12 QA05TIS12 QA05TIS12 SRM 2977

PCBs Lab 14 Lab 15 Exercise Assigned Lab 14 Lab 15 (ng/g dry mass) mean RSD mean RSD Value s 95% CL mean RSD From 2977 Certificate of Analysis PCB 8 3.02 7.99% 3.10 5.65% 2.56 0.60 0.75 SRM 1974b 1.84 22.70% 2.1 0.15 CertifiedPCB 18 8.22 17.05% 6.68 2.40% 5.71 1.87 1.34 SRM 1974b 2.81 24.14% 2.65 0.3 Certified

PCB 28 26.1 4.87% 30.4 8.21% 23.2 4.6 3.3 SRM 1974b 5.92 9.23% 5.37 0.44 CertifiedPCB 31 19.8 3.86% 29.4 6.54% 21.7 3.1 3.2 SRM 1974b 3.47 12.17% 3.92 0.24 CertifiedPCB 44 35.7 1.65% 36.8 6.00% 31.4 11.2 8.0 SRM 1974b 3.70 5.73% 3.25 0.63 CertifiedPCB 49 NA 50.5 4.12% 37.2 11.3 8.7 SRM 1974b <0.71 no target TargetPCB 52 65.0 1.78% 58.7 4.24% 46.6 10.7 7.6 SRM 1974b 10.8 5.52% 8.37 0.54 CertifiedPCB 66 58.4 1.96% 56.0 1.07% 48.4 12.4 8.3 SRM 1974b 2.79 11.27% 3.64 0.32 CertifiedPCB 95 66.1 7.41% 49.9 7.38% 51.5 9.2 8.5 SRM 1974b 6.47 10.40% 5.39 0.59 CertifiedPCB 99 59.5 5.30% 57.6 2.42% 47.0 7.2 6.7 SRM 1974b 2.31 9.50% no target TargetPCB 101 123 5.71% 102 1.73% 88.1 14.4 11.1 SRM 1974b 11.0 7.71% 11.2 1.2 CertifiedPCB 105 39.1 2.31% 28.5 0.76% 31.1 5.3 3.8 SRM 1974b 2.69 7.09% 3.76 0.49 CertifiedPCB 118 112 3.77% 106 1.03% 79.9 15.9 11.4 SRM 1974b 11.1 3.32% 10.5 1 CertifiedPCB 128 17.2 3.07% 13.7 2.52% 13.0 2.2 1.7 SRM 1974b 1.71 14.94% 2.49 0.28 CertifiedPCB 138 102 3.48% 106 2.17% 64.1 14.9 15.7 SRM 1974b 12.4 1.90% no target TargetPCB 149 65.3 5.05% 43.9 3.30% 61.5 11.2 11.7 SRM 1974b 5.94 8.94% 9.23 0.12 CertifiedPCB 153 110 3.14% 152 2.03% 85.7 29.6 21.2 SRM 1974b 17.7 10.24% 14.1 1 CertifiedPCB 156 5.45 2.86% 12.2 1.86% 5.64 1.25 1.16 SRM 1974b 1.11 13.01% 0.96 0.085 CertifiedPCB 170 2.50 0.88% 2.13 42.55% 1.80 0.38 0.29 SRM 1974b 2.97 10.75% 2.95 0.23 CertifiedPCB 180 9.57 0.86% 7.97 13.34% 9.29 3.11 2.39 SRM 1974b 5.70 10.45% 6.79 0.67 CertifiedPCB 187 25.7 2.29% 24.8 3.51% 18.0 4.0 2.7 SRM 1974b 4.05 6.78% 4.76 0.38 CertifiedPCB 194 <0.732 0.967 5.44% 0.501 0.084 0.105 SRM 1974b 0.657 3.16% 0.897 0.042 CertifiedPCB 195 <2.13 <0.8 no target SRM 1974b <0.8 no target TargetPCB 206 <0.866 <0.87 no target SRM 1974b <0.87 no target TargetPCB 209 <0.973 <0.73 no target SRM 1974b <0.73 no target Target
QA05TIS12 QA05TIS12 QA05TIS12 SRM 2977

BDEs Lab 14 Lab 15 Exercise Assigned Lab 14 Lab 15 (ng/g dry mass) mean RSD mean RSD Value s 95% CL mean RSD From 2977 Certificate of Analysis BDE 15 NA 0.433 48.04% no target SRM 1974b 0.5 no targetBDE 17 6.23 22.63% 0.767 19.92% 3.72 0.66 1.64 SRM 1974b 0.2 no target typeBDE 25 NA 3.93 5.87% no target SRM 1974b 1.5 no target TargetBDE 28 2.05 14.35% 0.500 20.00% 3.08 1.58 1.97 SRM 1974b 0.2 no target TargetBDE 30 NA <8.1 no target SRM 1974b <8.1 no target TargetBDE 33 NA 0.533 75.78% no target SRM 1974b 0.2 no target TargetBDE 47 30.4 2.64% 9.63 10.08% 23.3 5.4 6.7 SRM 1974b 1.7 no target TargetBDE 49 NA 0.500 34.64% 6.75 2.00 4.97 SRM 1974b 0.4 1.02 0.03 TargetBDE 66 <1.13 36.2 11.35% 0.984 0.403 0.642 SRM 1974b 48.6 0.375 0.062 TargetBDE 71 <1.18 NA no target SRM 1974b NA no target TargetBDE 75 NA 0.633 32.87% no target SRM 1974b <8.5 0.166 0.013 TargetBDE 85 <6.15 1.03 11.17% 0.418 0.074 0.185 SRM 1974b 0.6 no target TargetBDE 99 9.66 5.49% 0.767 27.15% 11.5 3.4 5.3 SRM 1974b 0.5 4.11 0.4 TargetBDE 100 7.12 8.69% 0.400 25.00% 6.85 1.93 2.40 SRM 1974b 0.4 1.06 0.18 TargetBDE 116 NA 16.3 17.30% no target SRM 1974b 6.4 no target TargetBDE 118 NA 1.13 51.70% no target SRM 1974b 0.4 no target TargetBDE 119 NA 9.17 40.82% no target SRM 1974b 1.5 no target TargetBDE 138 <3.13 4.40 47.73% no target SRM 1974b 0.7 no target TargetBDE 153 <1.00 2.67 31.89% 0.515 0.097 0.155 SRM 1974b 0.6 no target TargetBDE 154 <5.75 3.57 41.62% 0.550 0.103 0.164 SRM 1974b 0.7 no target TargetBDE 155 NA 1.17 40.51% no target SRM 1974b 0.8 no target TargetBDE 156 NA NA #DIV/0! no target SRM 1974b NA no target TargetBDE 181 NA 1.47 43.83% no target SRM 1974b 0.6 no target TargetBDE 183 <1.48 2.90 32.89% no target SRM 1974b 0.7 no target TargetBDE 190 6.17 0.00% 1.53 42.43% no target SRM 1974b 0.8 no target TargetBDE 191 NA NA no target SRM 1974b NA no target TargetBDE 196 NA NA no target SRM 1974b NA no target TargetBDE 197 NA NA no target SRM 1974b NA no target TargetBDE 203 NA NA no target SRM 1974b NA no target TargetBDE 205 NA NA no target SRM 1974b NA no target TargetBDE 206 NA NA no target SRM 1974b NA no target TargetBDE 207 NA NA no target SRM 1974b NA no target TargetBDE 208 NA NA no target SRM 1974b NA no target TargetBDE 209 NA NA no target SRM 1974b NA no target Target

Note only one sample of SRM 2977 analyzed by Lab 15 for BDE congeners
LAB NOTES RECEIVED WITH THE MUSSEL TISSUE DATA: 

Lab 14 notes:SRM 1974b analyzed instead of SRM 2977.Chrysene and triphenylene co-eluteBenzo(j)fluoranthene and benzo(k)fluoranthene co-elute PCB 8 was measured as cong 8+5.PCB 101was meaured as 101+90+89.PCB 118 measured as 118+106PCB 128 measured as 128+167PCB 138 measured as 164+163+138PCB 170 measured as 170+190Lab 15 notes:PCB101 coelutes with PCB90PCB153 coelutes with PCB132 and 168PCB170 coelutes with PCB190PCB 8 coelutes with PCB 5PCB 195 coelutes with PCB208<symbol refers to values less than our MDLthe chrysene number is the sum of chrysene and triphenylene, we cannot resolve the twowe do not analyze for benzo(j)fluoranthes because it coelutes with benzo(b)fluorantheneTissue MDL values are high due to limited sample volume and reporting data on a wet weight basis.Our MDLs are based on 13.0g wet weight of mussel. Therfore MDLs reported here are approximately 2X higher thanthose on 13 g wet. Additionally, sample PAH concentrations are low which is compounded by the small sample volume.BDE71 coelutes with BDE47
Three laboratories submitted data for QA05SED13 and the associated control sample after the first draft of this report

QA05SED13 QA05SED13 QA05SED13 QA05SED13 SRM 1941b SRM 1941b SRM 1941b

Lab 13 Lab 14 Lab 15 Exercise Assigned Lab 13 Lab 14 Lab 15

mean RSD mean RSD mean RSD Value s 95% CL mean RSD mean RSD mean RSD

Water (percent) 35.0 21.57% 45.7 46.6 0.99% 47.4 3.2 2.3 NA 0.0239TOC (percent) NA 2.88 0.36% 2.70 0.58 0.92 NA

QA05SED13 QA05SED13 QA05SED13 QA05SED13 SRM 1941b SRM 1941b SRM 1941b

PAHs Lab 13 Lab 14 Lab 15 Exercise Assigned Lab 13 Lab 14 Lab 15 From 1941b Certif. ng/g dry mass mean RSD mean RSD mean RSD Value s 95% CL mean RSD mean RSD mean RSD conc. 95%CL typenaphthalene 609 22.15% 881 6.61% 709 18.02% 785 186 172 859 5.27% 1006 9.23% 811 848 95 Certified2-methylnaphthalene 203 24.73% 322 3.12% 210 19.24% 219 66 61 265 1.57% 282 9.63% 264 276 53 Reference1-methylnaphthalene 82.6 19.68% 131 5.04% 104 17.61% 98.2 24.0 20.0 113 2.56% 134 12.41% 135 127 14 Referencebiphenyl 34.3 16.98% 82.7 2.22% 60.9 15.08% 65.8 23.6 21.8 56.5 14.42% 83.5 2.76% 57.4 74 8 Reference2,6-dimethylnaphthalene 58.3 20.06% 87.4 11.83% 50.6 19.48% 81.3 43.6 40.3 94.4 6.70% 96.6 18.81% 101 75.9 4.5 Referenceacenaphthylene 42.1 19.26% 59.3 4.89% 52.9 17.09% 45.1 13.8 11.5 57.5 9.37% 59.0 11.86% 69.7 53.3 6.4 Referenceacenaphthene 20.5 17.33% 42.3 11.46% 25.8 12.36% 28.9 8.4 7.0 28.6 15.06% 43.2 3.94% 28.5 38.4 5.2 Reference1,6,7-trimethylnaphthalene NA 73.3 4.81% 16.8 20.24% no target NA 62.2 3.22% 17.8 25.5 5.1 Referencefluorene 41.4 27.33% 79.9 10.19% 53.8 16.71% 56.1 12.2 10.2 80.3 11.94% 76.5 11.50% 61.4 85 15 Certifiedphenanthrene 262 22.42% 440 5.38% 350 13.34% 306 89 75 430 4.42% 458 7.45% 376 406 44 Certifiedanthracene 125 17.46% 209 3.92% 141 17.75% 137 47 40 184 12.89% 209 3.65% 185 184 18 Certified1-methylphenanthrene 55.5 26.51% 84.4 2.53% 53.8 10.19% 55.4 18.2 19.1 70.8 8.40% 83.9 4.43% 69.8 73.2 5.9 Certifiedfluoranthene 521 12.10% 721 3.64% 560 8.24% 496 140 117 841 0.89% 711 8.35% 567 651 50 Certifiedpyrene 445 18.36% 598 2.80% 451 9.97% 421 142 118 693 4.41% 585 9.61% 491 581 39 Certifiedbenz[a]anthracene 198 14.48% 432 1.75% 295 9.58% 241 80 67 287 8.26% 454 1.60% 334 335 25 Certifiedchrysene 267 19.16% 377 5.12% 410 9.68% 219 62 65 415 4.94% 368 9.12% 393 291 31 Certifiedtriphenylene NA NA no target NA NA 108 5 Certifiedbenzo[b]fluoranthene 279 14.07% 403 8.48% 567 8.38% 413 174 183 488 2.38% 387 5.92% 444 453 21 Certifiedbenzo[j]fluoranthene NA 432 6.02% NA no target NA 409 9.89% NA 217 5 Referencebenzo[k]fluoranthene 265 12.82% 213 7.48% 180 56 47 378 3.21% 204 225 18 Certifiedbenzo[e]pyrene 205 13.71% 281 4.49% 301 6.20% 286 97 75 330 3.74% 311 12.68% 336 325 25 Certifiedbenzo[a]pyrene 188 14.32% 350 9.62% 287 8.66% 282 120 111 278 5.89% 454 5.27% 339 358 17 Certifiedperylene 277 13.01% 416 3.30% 399 13.09% 311 131 138 364 2.33% 410 1.76% 345 397 45 Certifiedindeno[1,2,3-cd]pyrene 153 9.90% 353 6.36% 342 15.22% 258 101 85 241 13.62% 360 4.98% 436 341 57 Certifieddibenz[a,h]anthracene 40.7 26.35% 78.5 18.14% 41.9 21.2 33.8 47.9 10.74% 76.9 53 10 Certifiedbenzo[ghi]perylene 167 10.15% 276 3.02% 277 12.41% 244 96 81 278 10.38% 274 7.69% 312 307 45 Certified

Note: Only one sample of SRM 1941b was analyzed by Lab 15
QA05SED13 QA05SED13 QA05SED13 QA05SED13 SRM 1941b SRM 1941b SRM 1941b

Pesticides Lab 13 Lab 14 Lab 15 Exercise Assigned Lab 13 Lab 14 Lab 15 From 1941b Certif. ng/g dry mass mean RSD mean RSD mean RSD Value s 95% CL mean RSD mean RSD mean RSD conc. 95%CL typealpha-HCH (a-BHC) 0.032 26.90% NA <0.93 no target 0.036 NA <0.33 no target Targethexachlorobenzene 4.42 14.33% NA 5.83 10.24% 5.38 1.90 2.00 9.80 1.89% NA 7.96 5.83 0.38 Certifiedgamma-HCH (g-BHC,lindane) 0.035 20.44% NA 1.08 5.28% no target 0.042 NA <0.18 no target Targetbeta-HCH (b-BHC) 0.025 28.28% NA <0.76 no target 0.033 NA <0.27 no target Targetheptachlor 0.014 57.08% NA <0.68 no target 0.018 NA <0.24 no target Targetaldrin 0.033 18.48% NA <0.54 no target 0.042 NA <0.19 no target Targetheptachlor epoxide 0.025 38.91% NA <0.68 no target 0.041 NA 1.66 no target Targetoxychlordane 0.003 4.68% NA <0.91 no target 0.009 NA 0.458 no target Targetgamma-chlordane 0.321 19.14% NA <0.5 0.572 0.148 0.155 0.561 2.52% NA <0.18 0.566 0.093 Certified2,4'-DDE 0.184 15.72% <0.264 0.064 19.03% 0.380 0.103 0.256 0.288 15.71% <0.420 0.247 0.38 0.12 Referenceendosulfan I NA NA <1.06 no target NA <0.37 no target Targetcis-chlordane (alpha-chlordane) 0.329 24.21% NA 0.252 19.53% 0.482 0.058 0.072 0.527 6.35% NA 0.822 0.85 0.11 Certifiedtrans-nonachlor 0.137 20.82% NA 0.175 6.47% 0.286 0.089 0.093 0.238 1.99% NA 0.341 0.438 0.073 Certifieddieldrin 0.266 19.62% NA 0.728 8.73% 0.386 0.034 0.084 0.408 10.71% NA 0.810 no target Target4,4'-DDE 1.76 18.86% 5.63 5.90% 3.71 9.26% 3.44 1.08 1.00 2.95 6.62% 4.84 17.28% 3.45 3.22 0.28 Certified2,4'-DDD 0.237 11.07% <1.06 1.31 16.57% 0.927 0.624 0.992 0.412 2.81% <0.786 2.21 no target Targetendrin 0.037 NA <2 no target <0.907 NA <0.7 no target Targetendosulfan II NA NA <1.43 no target NA <0.5 no target Target4,4'-DDD 2.29 17.94% 3.10 4.17% 5.49 11.95% 4.18 1.42 1.77 3.51 6.35% 3.08 11.11% 4.01 4.66 0.46 Certified

2,4'-DDT 0.052 17.25% <1.19 no target 0.079 <1.90 0.223 no target Targetcis-nonachlor 0.082 21.26% NA 0.177 5.79% 0.454 0.493 1.224 0.166 11.36% NA 0.493 0.378 0.053 Certified4,4'-DDT 0.273 2.65% <2.65 0.825 7.43% 0.537 0.174 0.433 0.492 61.32% <4.22 1.69 1.12 0.42 Referencemirex 0.010 35.07% NA 0.063 6.48% no target 0.037 68.01% NA <0.27 no target Targetendosulfan sulfate NA NA <0.69 no target NA NA <0.24 no target Targetchlorpyrifos NA NA 0.461 26.44% no target NA NA 2.07 no target Target

Note: Only one sample of SRM 1941b was analyzed by Lab 15
QA05SED13 QA05SED13 QA05SED13 QA05SED13 SRM 1941b SRM 1941b SRM 1941b

PCBs Lab 13 Lab 14 Lab 15 Exercise Assigned Lab 13 Lab 14 Lab 15 From 1941b Certif. ng/g dry mass mean RSD mean RSD mean RSD Value s 95% CL mean RSD mean RSD mean RSD conc. 95%CL typePCB 8 0.697 20.61% 2.34 11.16% <1.09 1.36 0.42 0.39 0.920 2.78% 2.01 14.43% 1.23 1.65 0.19 CertifiedPCB 18 1.18 18.55% 3.01 6.72% 2.00 15.71% 2.04 0.48 0.37 1.64 6.93% 2.93 25.53% 3.37 2.39 0.29 CertifiedPCB 28 2.30 32.13% 6.00 2.27% 5.40 9.36% 3.79 0.97 0.81 3.15 2.40% 5.66 4.80% 5.85 4.52 0.57 CertifiedPCB 31 2.04 35.01% 3.56 5.26% 2.96 10.91% 2.83 0.94 0.87 2.71 2.16% 3.23 8.66% 3.86 3.18 0.41 CertifiedPCB 44 2.75 16.23% 4.15 6.30% 3.75 6.34% 3.47 0.99 0.76 5.51 2.46% 4.09 1.94% 4.65 3.85 0.2 CertifiedPCB 49 1.87 19.11% NA 4.00 0.32% 3.64 1.22 1.02 3.01 2.53% NA 5.03 4.34 0.28 CertifiedPCB 52 2.80 24.26% 7.28 4.99% 5.26 0.63% 4.48 1.39 1.07 4.22 2.05% 6.23 10.97% 6.84 5.24 0.28 CertifiedPCB 66 2.93 29.68% 4.10 2.13% 5.19 3.96% 4.32 1.41 1.18 4.51 3.92% 4.27 5.47% 6.59 4.96 0.53 CertifiedPCB 95 1.68 15.33% 5.61 7.03% 3.93 12.81% 3.44 1.25 1.05 2.63 1.66% 5.28 5.12% 4.20 3.93 0.62 CertifiedPCB 99 1.41 16.62% 3.59 13.44% 2.54 8.27% 2.45 0.87 0.73 2.28 4.94% 3.62 3.33% 2.92 2.9 0.36 CertifiedPCB 101 2.42 17.44% 7.12 6.18% 6.01 12.97% 4.86 1.36 1.05 3.78 0.55% 6.83 6.55% 6.37 5.11 0.34 CertifiedPCB 105 0.572 14.99% 1.37 2.48% 1.93 19.91% 1.26 0.40 0.31 0.95 2.20% 1.39 9.80% 1.27 1.43 0.1 CertifiedPCB 118 1.81 16.14% 4.52 10.86% 4.23 15.38% 3.59 1.26 0.96 2.94 1.61% 4.59 12.89% 4.69 4.23 0.19 CertifiedPCB 128 0.361 17.58% 0.832 14.73% 0.433 14.12% 0.684 0.275 0.197 0.63 3.87% 0.776 14.46% <0.47 0.696 0.044 CertifiedPCB 138 2.37 17.51% 5.47 4.67% 3.80 7.69% 3.83 1.23 1.14 4.01 4.64% 5.42 5.77% 4.79 3.6 0.28 CertifiedPCB 149 1.94 15.08% 5.32 5.55% 1.83 5.40% 4.18 1.44 1.20 3.20 1.90% 5.10 3.76% 5.23 4.35 0.26 CertifiedPCB 153 2.50 20.27% 6.04 4.34% 6.23 6.20% 4.99 2.46 2.06 4.05 2.34% 5.89 3.07% 7.19 5.47 0.32 CertifiedPCB 156 0.231 18.71% 0.421 11.59% 2.12 8.72% 0.444 0.128 0.107 0.374 0.77% 0.369 3.10% 1.20 0.507 0.09 CertifiedPCB 170 0.553 19.78% 1.35 6.57% 2.23 3.50% 1.19 0.36 0.28 0.926 3.09% 1.36 9.51% 1.49 1.35 0.09 CertifiedPCB 180 1.50 17.22% 3.43 7.54% 4.35 4.16% 2.97 1.15 0.83 2.57 2.72% 3.34 1.77% 4.73 3.24 0.51 CertifiedPCB 187 0.761 18.78% 2.51 3.62% 2.49 23.24% 2.20 0.69 0.49 1.33 4.39% 2.45 0.92% 3.02 2.17 0.22 CertifiedPCB 194 0.413 21.17% 0.972 8.30% <0.43 1.01 0.50 0.46 0.693 1.52% 0.982 2.55% 1.30 1.04 0.06 CertifiedPCB 195 0.141 22.97% <0.850 1.06 20.14% 0.291 0.166 0.174 0.244 8.13% <1.36 0.881 0.645 0.06 CertifiedPCB 206 NA 2.73 6.89% 2.95 7.31% 1.91 0.66 0.55 NA 2.83 1.85% 3.36 2.42 0.19 CertifiedPCB 209 NA 5.92 7.59% 7.35 6.77% 4.02 1.47 1.36 NA 6.05 1.82% 6.65 4.86 0.45 Certified

Note: Only one sample of SRM 1941b was analyzed by Lab 15
QA05SED13 QA05SED13 QA05SED13 QA05SED13 SRM 1941b SRM 1941b SRM 1941b

BDEs Lab 13 Lab 14 Lab 15 Exercise Assigned Lab 13 Lab 14 Lab 15 From 1941b Certif. ng/g dry mass mean RSD mean RSD mean RSD Value s 95% CL mean RSD mean RSD mean RSD conc. 95%CL typeBDE 15 0.126 15.62% NA 0.967 11.66% no target 0.185 8.87% NA NA no target TargetBDE 17 0.039 10.24% <0.357 <0.44 no target 0.069 34.37% <0.586 NA no target TargetBDE 25 oelute (17/25) NA <0.44 no target coelute (17/25) NA NA no target Target

BDE 28 0.036 8.83% <0.532 <0.27 0.056 0.005 0.042 0.135 5.47% <0.847 NA 0.18 0.07 TargetBDE 30 0.028 55.16% NA <0.44 no target 0.047 10.47% NA NA no target TargetBDE 33 oelute (28/33) NA <0.44 no target coelute (28/33) NA NA w/ BDE 28 TargetBDE 47 0.380 23.61% 0.890 2.44% 1.09 9.53% 1.03 0.35 0.86 1.34 7.79% 1.87 6.21% NA 1.48 0.51 TargetBDE 49 0.091 24.31% NA <0.74 no target 0.133 74.26% NA NA no target TargetBDE 66 0.023 9.58% <0.450 <1.41 0.038 0.009 0.078 0.045 4.56% <0.716 NA no target TargetBDE 71 0.008 1.34% <0.471 NA no target 0.167 32.97% <0.750 NA no target TargetBDE 75 0.012 13.92% NA <0.74 no target 0.019 30.29% NA NA no target TargetBDE 85 0.011 94.73% <2.46 <0.58 no target 0.025 <3.91 NA no target TargetBDE 99 0.252 26.15% 0.710 20.83% 0.833 22.72% no target 0.398 12.88% 0.667 54.24% NA 0.62 0.19 TargetBDE 100 0.060 23.85% <0.418 <0.78 no target 0.103 13.83% <0.665 NA 0.15 0.06 TargetBDE 116 <0.00291 NA <0.78 no target <0.00309 NA NA no target TargetBDE 118 NA NA <0.78 no target NA NA NA no target TargetBDE 119 0.003 NA <0.78 no target 0.035 79.52% NA NA no target TargetBDE 138 0.009 18.40% <1.25 <0.65 no target 0.007 3.24% <1.99 NA no target TargetBDE 153 0.044 47.80% 0.121 19.78% <1.93 no target 0.064 9.30% 0.114 35.29% NA 0.09 0.04 TargetBDE 154 0.034 19.85% 0.207 8.27% <0.95 no target 0.058 7.27% 0.199 11.19% NA 0.09 0.02 TargetBDE 155 0.007 41.31% NA <0.95 no target 0.009 NA NA no target TargetBDE 156 NA NA NA no target NA NA NA no target TargetBDE 181 < 0.00961 NA <1.41 no target <0.00325 NA NA no target TargetBDE 183 0.064 132.57% <0.384 <1.41 no target 0.026 20.98% <0.943 NA 0.05 0.02 TargetBDE 190 0.011 62.39% <2.47 <1.65 no target 0.002 <3.93 NA no target TargetBDE 191 NA NA NA no target NA NA NA no target TargetBDE 196 NA NA NA no target NA NA NA no target TargetBDE 197 NA NA NA no target NA NA NA no target TargetBDE 203 0.044 83.65% NA NA no target 0.065 37.74% NA NA no target TargetBDE 205 <0.0524 NA NA no target <0.0236 NA NA no target TargetBDE 206 0.256 28.83% NA NA no target 0.374 14.59% NA NA no target TargetBDE 207 0.153 34.92% NA NA no target 0.215 26.25% NA NA no target TargetBDE 208 0.119 28.78% NA NA no target 0.200 45.62% NA NA no target TargetBDE 209 11.4 16.11% NA NA no target 21.2 14.24% NA NA 24.11 14.97 Target

Note: No data for Lab 15 on SRM 1941b
LAB NOTES SUBMITTED WITH THE SEDIMENT DATA

Lab 13 Notes:

other= ion ratio did not meet method criteria

other= coelution

for PBDEs, PCBs and pesticides, samples were concentrated down to 100 uL

QA05SED13 SRM 1941b

Lab 13 Lab 13

mean RSD mean RSD

C2-NAPHTHALENES 277 23.56% 246 49.62%

C1-NAPHTHALENES 290 24.87% 296 53.63%

2,3,5-TRIMETHYLNAPHTHALENE 22.0 21.66% 23.6 22.67%

C1-CHRYSENES 141 4.52% 188 14.71%

C1-DIBENZOTHIOPHENES < 3.5 23.9

C1-FLUORANTHENES/PYRENES 244 20.77% 312 2.72%

C1-FLUORENES < 3.5 116

C1-PHENANTHRENES/ANTHRACENES 308 1.95% 312 52.09%

C2-CHRYSENES < 3.5 <10

C2-DIBENZOTHIOPHENES < 3.5 <10

C2-FLUORENES < 3.5 773

C2-PHENANTHRENES/ANTHRACENES 327 9.88% 357

C3-CHRYSENES < 3.5 <10

C3-DIBENZOTHIOPHENES < 3.5 85.3

C3-FLUORENES < 3.5 <10

C3-NAPHTHALENES 190 23.91% 94.6 61.75%

C3-PHENANTHRENES/ANTHRACENES < 3.5 <10

C4-CHRYSENES < 3.5 10.9

C4-NAPHTHALENES < 3.5 110

C4-PHENANTHRENES/ANTHRACENES < 3.5 23.6

DIBENZOTHIOPHENE 30.3 16.95% 45.8 26.25%

IUPAC# 33 0.975 35.52% 1.35 1.96%

IUPAC# 56 1.20 30.86% 1.76 2.56%

IUPAC# 60 0.264 34.31% 0.381 1.24%

IUPAC# 70/74 4.15 32.35% 6.06 3.07%

IUPAC# 87/97 1.27 13.59% 2.20 0.94%

IUPAC# 110 2.67 12.36% 4.53 1.96%

IUPAC# 132 0.588 16.54% 1.00 3.11%

IUPAC# 141 0.345 20.29% 0.588 7.43%

IUPAC# 151 0.896 15.68% 1.47 3.74%

IUPAC# 158 0.196 17.00% 0.322 3.11%

IUPAC# 174 0.657 20.50% 1.07 3.88%

IUPAC# 177 0.302 16.82% 0.536 3.17%

IUPAC# 183 0.442 18.51% 0.741 2.51%

IUPAC# 201 0.098 19.35% 0.174 2.07%

IUPAC# 203 0.344 18.72% 0.619 2.18%

DELTA BHC 0.016 63.46% <0.44

PBDE # 7 0.031 11.98% 0.043 3.93%

PBDE # 8/11 0.032 16.85% 0.043 15.20%

PBDE # 10 0.002 23.60% 0.002 12.86%

PBDE # 12/13 0.012 6.22% 0.015 1.72%

PBDE # 32 0.007 27.24% 0.046 5.58%

PBDE # 35 0.008 16.42% 0.103 154.61%

PBDE # 37 0.006 18.82% 0.007 22.39%

PBDE # 77 0.003 11.45% 0.001

PBDE # 79 0.002 0.005

PBDE # 105 < 0.00723 <0.00588

PBDE # 126 < 0.00232 0.011 4.00%

PBDE # 128 0.004 <0.00543

PBDE # 140 0.003 0.003

PBDE # 204 0.056 124.34% 0.047 47.04%
Lab 14 notes:

Chrysene and triphenylene co-elute

Benzo(j)fluoranthene and benzo(k)fluoranthene co-elute 

PCB 8 was measured as cong 8+5.

PCB 101was meaured as 101+90+89.

PCB 118 measured as 118+106

PCB 128 measured as 128+167

PCB 138 measured as 164+163+138

PCB 170 measured as 170+190

Lab 15 notes:

the crysene number is the sum of chrysene and triphenylene, we cannot resolve the two

we can not separate benzo(j)fluoranthene because under our conditions it coelutes with 

 benzo(b)fluoranthene

<symbol refers to values less than our MDL

PCB101 coelutes with PCB90

PCB153 coelutes with PCB132 and 168

PCB170 coelutes with PCB190

PCB8 coelutes with PCB5

PCB195 coelutes with PCB208

Total carbon and total organic carbon are measured independently in oven-dried sediments 

 and soils using a LECO CR-412 Carbon Determinator

BDE71 coelutes with BDE47
Appendix J: List of Laboratories Participating 

in 2005 Intercomparison Exercises

For this exercise, data were received from the following laboratories within the required 

timeframe. (This listing does NOT correspond to the laboratory number identification codes 

used in this report which were assigned in order of receipt of data with the exception of NIST 

which is Laboratory #1 in this exercise. The same code was used for both exercises.) 

Academy of Natural Sciences 

1900 Benjamin Franklin Parkway 

Philadelphia, PA 19103 

Jeffrey Ashley, Linda Zaoudeh, and Mike Schafer 

Alpha Woods Hole Laboratories 

375 Paramount Dr, Suite B 

Raynham, MA 02767 

Pete Kane and Elizabeth Porta 

AXYS Analytical 

2045 Mills Rd West / PO Box 2219 

Sidney, BC V8L 3S8 

Canada 

Dale Hoover 

Battelle Columbus 

505 King Ave 

Columbus, OH 43201 

Karen Tracy and Mary Schrock 

Battelle Duxbury Operations 

397 Washington Street 

Duxbury, MA 02332 

Carole-Sue Peven McCarthy 

East Bay Municipal Utility District 

2020 Wake Avenue 

Oakland, CA 94607 

Saskai van Bergen and Francois Rodigari 

Environment Canada 

Environmental Science Center 

Corner Morton & Université Ave 

Moncton, NB E1A3E9 Canada 

Jamie Aubé 
Massachusetts Water Resources Authourity 

100 Tafts Ave. 

Winthrop, MA 02152 

Jennifer Prasse 

NIST 

100 Bureau Drive, Stop 8392 

Gaithersburg, MD 20899-8392 

Michele Schantz 

NIST-Charleston Laboratory 

331 Fort Johnson Road 

Charleston, SC 29412-9110 

John Kucklick, Stacy Vander Pol, and Aurore Guichard 

NOAA Fisheries / ABL 

11305 Glacier Hwy 

Juneau, AK 99801 

Marie Larsen 

NOAA-NMFS 

2725 Montlake Boulevard, East 

Seattle, WA 98112 

Donald Brown / Jennie Bolton 

NOAA-NOS 

Hollings Marine Laboratory 

331 Fort Johnson Road 

Charleston, SC 29412 

Ed Wirth 

STL Sacramento 

880 Riverside Pkwy 

West Sacramento, CA 95605 

Michael Flournoy 

TDI-Brooks International 

B&B Laboratories 

1902 Pinon 

College Station, TX 77845 

Juan Ramirez 
Wadsworth Center, NYSDOH 

Empire State Plaza 

P-1 North Dock (Rm D520) 

Albany, NY 12237 

Chia-Swee Hong