### 1 OFFICE OF CHEMICAL SAFETY AND POLLUTION PREVENTION **UNITED ST AT**  **ES ENVIRONMENT AL**  **PROTECTION A GENCY** 

**WA SHINGTON D.C., 20460** 

**PC Code** : 114004 **DP Barcodes** : D384516 **Date** : August 30 , 2010 

### MEMORANDUM **SUBJECT** : Section 3 Registration for New U se of Amicarbazone on Turf Grasses Grown

Nationwide
. **TO** : Kathry

n Montague , Risk Manager (RM 23) Michael Walsh, Team Reviewer Product Registration Division (7505P) **FROM** : Stephen Carey, Biologist

Andrew Shelb y, Physical Scientist Environmental Risk Branch 6 Environmental Fate and Effects Division (7507P ) **THROUGH:** Mark Corbin , Branch Chief

Environmental Risk Branch 6 Environmental Fate and Effects Division (7507P) This memorandum transmits the Section 3 Ecological and Environmental Risk Conclusions, based on the Environmental Fate and Ecotoxicity Assessments for the proposed new use on turfgrasses to the labeling for Amicarbazone DF Herbicide (EPA Reg. No. 66330
- 46 ) to allow its use on golf courses, sod farm, school grounds, commercial and recreational turf grass, including residential turf grass and other turf areas and conifers in nurseries and field plantings, including Christmas trees
. The maximum proposed annual application rate at 0.44 lb a.i./A per ye ar is slightly lower than the current maximum annual rate, registered for application to field corn and corn grown for silage at 0.45 lb a.i./A per year . Additionally, the registrant has submitted conditional ecological effects data for the active ingredient on chronic toxicity to estuarine/marine fish (MRID 48237510) and acute dietary to bobwhite quail (MRID 48237511) ; data for the metabolites of amicarbazone (both desamino and N
-methyl desamino) on bobwhite quail (MRIDs 48237514 & 48237515) and mallard reproduction (MRIDs 48237512 & 48237513) and on the emergence (MRID 48237516 & 48237517) and vigor (MRIDs 48237518, 48237519 & 48237520) of terrestrial plants required for registration. The study classification and results of the newl y submitted data, including the data evaluation reports (DERs) are attached in

### 2

**Appendix VI** 
. E cological endpoints have not changed with the additional data except for acceptable endpoints for chronic toxicity to estuarine/marine fish and dietary toxicity to bobwhite quail , which did not impact the risk conclusions . Also, data on the metabolites suggests they are less toxic than the parent compound ; thus, all ecological endpoints used in the assessment remain the same with the parent compound as the most toxic material which does not alter risk conclusions from the previous assessment
. Inthat assessment, amicarbazone was found to present potential chronic risk to birds and mammals. There is also the potential for risk to both terrestrial and aquatic plants . Minimal risk was found for fish and aquatic invertebrates
. Further characterization can be found in the New Chemical assessment (DP Barcode D288631 ; USEPA 2005 ). **1. SUMMARY** 

**– USE CHARACTERIZATION**  Amicarbazone belongs to the triazolinone class of compounds and is a photosystem II (PS II) inhibitor with burn
- down activity
. This portion of the photosy nthesis cycle uses light energy to drive two chemical reactions: the oxidation of water and the reduction of plastoquinone. No specific information is available on the mechanism of action of amicarbazone which affects the PS II process. Current/proposal label language indicates that this herbicide is not to be use d on coarse - textured soils (sand, loamy sand and sandy l oam soils), or on soils with pH >7.4, such as calcareous soils. Amicarbazone is proposed to be applied as broadcast ground spray or via spot treatment using backpack sprayers at maximum rate s of 1.0
- 10. 0 oz formulated product/acre/year (0.044
- 0.44 lb a.i/acre/ye ar). The shortest reapplication interval is 7 days. A nonionic surfactant with at least 80% a.i. should be added at 0.25% v/v to the spray mix
. Amicarbazone can be used as part of tank
-mixes to c ontrol emerged weeds. The proposed maximum single application rate for turf is slightl y less than the maximum single rate for currentl y registered corn uses . Corn uses of amicarbazone currentl y registered are included in **Table 1**  for comparison
. More infor mation on the use characterization for corn can be found in D288631 (USEPA 2005)
. **Table 1. Summary of C urrent and**  **P roposed**  **U ses of**  **Amicarbazone**

**USE MAX SINGLE** 

**APP. RATE (lbs. a.i./A)**  **NUMBER OF** 

**APPS. SEASONAL** 

**APP. RATE (lbs. a.i./A)**  **APPLICATION**

**ME THOD**  Proposed new use: Turf grasses and Conifers 0.44 1 0.44 Ground Spray Current uses: Corn 0.45 1 (full) or 2 (split) 0.45 Ground Spray 

### 2. **SUMMARY**

**– ENVIRONMENTAL FATE**  Amicarbazone is moderately persistent in the environment as it eventuall y transforms b y biotic mechanisms (t1⁄2= 87 days ; aerobic soil metabolism). Under acidic and neutral environments, amicarbazone is stable to hydrol ys is, but transforms slowly under alkaline conditions. Photolysis

### 3

also occurs slowly

for amicarbazone in water and on soil with half
- lives on the order of about two months, estimated for outdoor environmental conditions. The primary transformation products of amicarbazone are **Des -**  **am ino:**  N
- (1,1
- dimethylethyl)
- 4,5
- dihydro
- 3
- (1
-methylethyl )
- 5
- oxo - 1H
- 1,2,4
- triazole
- 1
- carboxamide; **N -**  **methyl Des -**  **am ino:**  N
- (1,1
- dimethylethyl)
- 4,5
- dihydro
- 4
-methyl
- 3
- (1
-methylethyl)
- 5
- oxo - 1H
- 1,2,4
- triazole
- carboxamide; and **Decarboxamide:** 4
- amino
- 2,4
- dihydro
- 5
- (1
-methylethyl)
- 3H
- 1,2,4
- triazol
- 3
- one. Amicarbazone has a low vapor pressure and Henry’s Law constant ; therefore, volatilization from water and soil surfaces is not expected to be an important environmental fate process. The moderate persistence and high mobility of amicarbazone in soil make l eaching of this compound to lower soil horizons and groundwater possible. Likewise, leaching and possible ground water contamination with amicarbazone’s major three metabolites are possible due to their apparent long persistence and high mobility
. Small s cale prospective groundwater studies have been requested and submitted to the Agency
. Amicarbazone is not to be applied on soils with pH values higher than 7.4. Therefore, the fate of the chemical under neutral and acidic conditions only is considered in this assessment. In such environments, two major amicarbazone transformation products (Des
- amino and N
-methyl Des
amino) are expected to be formed. In metabolism studies, these metabolites increase in concentration to the end of study
. Given the moderate persistence/high mobility and solubility

of the parent compound and the apparent high persistence/high mobility of the two transformation products, both amicarbazone and its first two transformation products are expected to dissipate slowly and at the same time be vulnerable to leaching/run
- off. Hydrol ys is studies on the des
amino and N
-methyl des
- amino metabolites indicate stability at environmental relevant temperatures and pH (MRIDs 48073601 and 48237506). If amicarbazone is transported into alkaline ground water, surface water bodies and/or soils, the third transformation product (Decarboxamide) may

be formed since this was the only major metabolite identified in the base
catal yz ed hydrol ytic transformation of amicarbazone. Parent amicarbazone only is considered in this assessment due to : 1) a freshwater green algae study indicating the des
- amino metabolite is less toxic than parent ; 2) four avian reproduction studies indicating the metabolites, des
- amino and N
-methyl, are less toxic than the p arent; and 3) seedling emergence and vegetative vigor studies indicating the metabolites, des
- amino and N
methyl, are less toxic than the parent
. To represent new uses on turf and conifers across the conterminous United States , nine PRZM scenarios were utilized with the Florida Nursery scenario resulting in the highest EE Cs of all scenarios
. **Table 2**  **. PRZM/EXAMS input parameter values for amicarbazone**  **use on turf and conifers PARAMETER (units)**  **VALUE(S) SOURCE**  **COMMENT** Application Rate (kg a.i./ha) Parent: 0.490 Product label; Represents the maximum possible single

application rate per crop season (year) for

all new uses.

### 4

**Table 2**  **. PRZM/EXAMS input parameter values for amicarbazone**  **use on turf and conifers PARAMETER (units)**  **VALUE(S) SOURCE**  **COMMENT** Number of Applications 1 Maximum single application rate equals maximum annual application . Multiple applications at lower rates are possible only a single application can be done at the

maximum rate Molecular weight (g/mol) Parent: 241 Product Chemistry - Henry’s Law Constant (atm
m3 /mol) 1.5 x 10 - 12 (Vapor Pressure [torr]/760)/(Solubility [mg/L]/Molecular Weight [g/mol]) Vapor Pressure (torr) 2.2 x 10 - 8

- Solubility in Water @ 20 O C, (mg/L or ppm) 4,600 Product Chemistry – Organic Carbon Partition Coefficient (mL/g) 16.7 45121719; 45121732; 45121801; 45121733 KOC ranges from 16.7 to 37.0. Lowest non
sand KOC used for modeling. KOC is correlated to KD (r2 = 0.62). CAM (Chemical Application Method) 1 ground spray; pre
- emergence, unincorporated Depth of Incorporation (inches) 0 Product label – Application efficiency (decimal) 0.99 Spray drift fraction (decimal) 0.01 Application date March 1 Spring applications are stipulated on the label for turf and conifers. Two spring applications were modeled. The indicated date resulted in highest EECs Hydrolysis Half

- life (days) Stable 45121730 Aqueous Photolysis Half
- life @ pH 7 (days) Stable 45121803 Aerobic Aquatic Metabolism Half
life (days) 140 2x Aerobic Soil Metabolism half
- life Anaerobic Aquatic Metabolism Half
- life (days) Stable 45121724 Aerobic Soil Metabolism Half
- life (days) 69.9 45121731; 48237507 90 th percentile average of 5 half
- life estimates : 36.7 days, 40.3 days, 42.8 days,

68 days and 87 days

### 5

Estimated environmental concentrations (EECs) resulting from the above input parameters are can be found in the following table. Re sults from the Florida Nursery scenario are used for risk estimation with EECs of 8.97 ppb (peak), 7.48 ppb (21
- day exposure), and 6.21 ppb (60
- day exposure). The previous assessment for use of amicarbazone on corn resulted in EECs of 33.78 ppb (peak), 33.53 ppb (21
- day exposure), and 29.14 ppb (60
- day exposure). PRZM/EXAMS output can be found attached in **Appendix III** 
. **Table 3. PRZM/EXAMS EECs for amicarbazone use on turf and conifers**  **Scenario Application** 

**date Peak (ppb)**  **21 -**  **day exposure (ppb)**  **60 -**  **day exposure**

**(ppb)** CA Nursery February 15 6.32 6.10 5.78 March 1 3.76 3.58 3.33 FL Turf February 15 0.47 0.46 0.43 March 1 0.49 0.46 0.42 FL Nursery February 15 5.17 4.70 4.29 March 1 **8.97 7.48**  **6.21** MINursery February 15 2.67 2.56 2.30 March 1 3.84 3.61 3.30 NJ Nursery February 15 4.79 4.58 4.38 March 1 5.07 4.94 4.67 OR Christmas tree February 15 1.11 1.07 1.00 March 1 0.80 0.78 0.75 OR Nursery February 15 1.99 1.94 1.84 March 1 1.68 1.64 1.57 PA Turf February 15 0.58 0.58 0.56 March 1 0.81 0.79 0.77 TN Nursery February 15 5.59 5.34 4.42 March 1 5.91 5.69 4.86 **3. SUMMARY** 

**– ECOLOGICAL EFFECTS**  Results of acute toxicity

studies indicate that technical grade amicarbazone (amicarbazone TGAI) is practicall y non- toxic to honey bees, slightly

toxic to mammals, and slightly toxic to practicall y non- toxic to birds. New data on the acute dietary with bobwhite quail indicates amicarbazone is practically non- toxic to birds (LC 50 

### >5690 mg a.i./kg diet). However, results of chronic toxicity

studies show that longer
- term exposure to amicarbazone TGAI and the two metabolites, desamino amicarbazone and N
-methyl desamino amicarbazone, result in adverse effects on growth and reproductive parameters in birds . In addition, exposing the TGAI to parents and offspring in mammals resulted in reduced growth parameters . No data are available to characterize the effects o f amicarbazone metabolites in mammals or insects ; however, the bird reproduction studies indicate the metabolites are 6 to 38 times less toxic than the parent compound
. No data with passerine birds are available; an acute oral passerine bird study is required to satisfy

EPA guideline requirements.

### 6

Results of Tier 2 seedling emergence and vegetative vigor terrestrial plant studies with the amicarbazone formulated product MKH 3586 70 WG show that both monocots and dicots are sensitive to exposure, with dic ots appearing more sensitive. Ti er 2 seedling emergence and vegetative vigor studies with the amicarbazone metabolite s (Des
- amino and N
-methyl ) exhibited some effects; however, both are approximately 2
- fold less toxic than the parent compound
. Toxicity studies demonstrate that amicarbazone TGAI is practically non - toxic on an acute basis to freshwater and estuarine/marine fish, slightl y toxic to freshwater invertebrates, and slightly toxic to practically

non- toxic to estuarine/marine invertebrate s. Longer
- term exposure of aquatic animals to amicarbazone produces adverse effects on growth parameters in freshwater fish and on growth and reproductive parameters in freshwater and estuarine/marine invertebrates. New data indicates longer
- term exposure of estuarine/marine fish to amicarbazone resulted in no effects as high as 2.3 mg a.i./L . No data are available to characterize the effects of amicarbazone metabolites in aquatic animals but with the parent persistent and stable to hydrolys is, the metab olites are expected to have a minimal impact on aquatic animals in acidic or neutral surface waters. However, the alkaline hydrolysis metabolite, Decarboxamide, may be formed in alkaline surface waters
. No data for the alkaline metabolite, Decarboxamide, i s available; however, because current/proposal label language prohibit applying on coarse - textured soils (sand, loamy sand and sandy loam soils), or on soils with pH >7.4, such as calcareous soils ; data on the effect of the alkaline metabolite to aquatic organisms is not required at this time. In the aquatic environment, amicarbazone appears to be particularly toxic to algae, but produces adverse effects on survival and growth in both algae and vascular plants. The results of a single study assessing the toxicity

of the amicarbazone metabolite Des
- amino in Selenastrum capricornutum (a freshwater green algae), indicate that Des
- amino is 16
- fold less toxic than the parent compound. An updated toxicity

profile for amicarbazone can be found in **Appendix I** 
. **4 .**  **SUMMARY**

**– RISK CONCLUSIONS**  The ecological assessment for amicarbazone as a new chemical has a slightly higher application rate, 0.45 lb a.i./A than the proposed new use: a maximum single application rate of 0.44 lb a.i./A, applied as a ground spray
. The crop scenarios for turf and conifers lead to slightl y lower exposure estimates, but the change is not significant enough to alter previous risk conclusions. Environmental exposure inputs and ecological endpoints have not changed significantl y, so the conclusions from the New Chemical assessment remain relevant for the current proposal (DP Barcode D 28 8631 )
. A complete endangered species anal ys is for these conclusions is included in that document as well ; a summary of the expected effects to listed sp ecies is provided below
. 4
. 1
. Risk to Aquatic Organisms and Plants The previous assessment as a New Chemical for amicarbazone concluded that there was negligible risk to aquatic organisms and vascular plants (duckweed) except for algae at a Tier II level, where exposure estimates are dependent on crop specific scenarios as well as on application rates. Additionally, newly submitted data with estuarine/marine fish indicate chronic

### 7

risks to the se organisms are expected to be low the levels of concern (**Appendix II)**
. Given negligible changes in the risk picture, the previous risk conclusions can still apply to the proposed new use , as presented in **Table 4** 
. The results of the new risk quotients (RQs) for aquatic organisms using scenarios for turf and conifers are lower than corn uses but do not alter the risk conclusions and can be found in **Appendix II**
. **TABLE 4**  **. Summary of Environmental Risk Conclusions for Aquatic Organisms**  **and Plants Assessment Endpoint**  **LOC Exceedances**  **Summarized Risk Characterization**
Ac ute Risk to Freshwater & Estuarine/Marine Fish and Invertebrates None **Acute RQs =** At the peak EECs, there are no exceedances of the Non
- listed Species Acute Risk, Acute Restricted Use, or Listed Species Ac ute Risk LOCs for freshwater and estuarine/marine fish or invertebrates. The acute toxicity values [ranging from 40,000 to 12 0 , 00 0 μg/L] are at least three orders of magnitude higher than the peak EEC
. Chronic Risk to Freshwater Fish and Invertebrates None **Chronic RQs =** There are no Listed and Non
- listed Chronic LOC exceedances for freshwater fish or invertebrates. The highest 21
- and 60
- day EECs in surface water are at least two orders of magnitude lower than the lowest toxicity values, for rainbow trout (NOAEC of 7300 μg/L) and daphnids ( L OAEC of 252 μg/L). **Uncertainty:** An uncertainty exists for chronic exposure of the TGAI to freshwater invertebrates because effects were observed at the lowest concentration in the test; thus, the non
- definitive NOAEC could not be used to assess risk to freshwater invertebrates. A closer look shows that a NOAEC would need to be 7.48 μg a.i./L or 34 times lower to yield an RQ that exceeds the Listed and Non
- listed Chronic LOC of 1. Chronic Risk to Estuarine/Marine Fish and Invertebrates None **Chronic RQs =** There are no Listed and Non
- listed Chronic LOC exceedances for freshwater fish or invertebrates. The highest 21
- and 60
- day EECs in surface water are at least two orders of magnitude lower than the lowest toxicity values, for Sheepshead minnow (NOAEC of 2300 μg/L) and mysids (NOAEC of 5000 μg/L). Risk to Aquatic Vascular Plants None **Vascular Plant**  **RQs =** At the peak EEC , there are no exceedances of the Non
- Listed Plant or Listed Plant LOCs for vascular plants inhabiting water bodies
. The non- listed plant and listed plant toxicity values [210 to 40.6 μg/L] average one order of magnitude higher than the highest peak EECs. Risk to Aquatic Non
Vascular Plants **L isted Plant**  **Non -** **Vascular Plant**  **RQs =** There is an exceedance of the listed plant LOC for non
- vascular plants with RQs as high as 1. 6 . The non
- listed plant LOC is not exceeded (RQ = 0. 5 )
.

### 8

4.2
. Risk to Terrestrial W ildlife and Plants For terrestrial plants and animals, risk conclusions are not crop dependent and so the conclusions for the corn use, summarized in **Table 5**  below, also apply to the proposed new use. Details of the risk conclusions and its calculations for the corn use risk assessment on birds, mammals and plants based on a maximum single applic ation rate of 0.4 484 lb a.i./A can be found in **Appendix I** **V** for birds and mammals and **Appendix V** for plants
. **TABLE 5**  **. Summary of Environmental Risk Conclusions for Terrestrial Wildlife and Plants**  **Assessment Endpoint LOC**  **Exceedances Summarized Risk C**  **haracteri zation** 
Ac ute Risk to Birds None **Acute RQs =** The acute RQs range <0.01 to <0.088 for all weight classes of birds consuming all feed types were less than the Listed and Non
- listed Ac ute LOCs, indicating risk adverse effects is minimal. Using the dietary
-based LC 50 , no Listed and Non
- listed Species Ac ute LOCs were exceeded (RQ s range <0.001 to <0.01)
. Chronic Risk to Birds **Listed and Non**  **** **listed Species Chronic LOC**  **Chronic RQs =**

Using the dietary
-based NOAEC , the Listed and Non
- listed Species Chronic LOC was exceeded for birds consuming short grasses at the maximum application rate of 0 .45 lb a.i./A (RQ = 3.97), broadleaf plants/small insects (RQ = 2.23), and tall grass (RQ = 1.82) and maximum predicted residues. No risks were observe d for birds feeding on fruit, pod s, seeds, large insects (RQ = 0.25)
. Ac ute Risk to Mammals None **Acute RQs =** The acute RQs range <0.001 to <0.043 for all weight classes of mammals consuming all feed types were less than the Listed and Non
- listed Ac ute LOC s , indicating risk adverse effects is minimal. Chronic Risk to Mammals **Listed and Non**  **** **listed Species**  **and Chronic LOCs**  **Chronic RQs =**

At maximum predicted residue levels and maximum application rate of 0.45 lb a.i./A , the dose
-based RQs exceeded the Listed and Non
- listed Species Chronic LOC for all weight classes of mammals (15, 35, 1000 g) consuming short grasses (RQs range 3.28 to 7.27) , tall grasses (RQs range 1.5

– 3.33) and broadleaf forage/small insects (RQs range 1.8 4 to 4.09)
. No risks were observed for mammals feeding on fruits, pods, seeds, large insects (RQs range 0.05

– 0.45)
. Dietary
-based RQs exceed the Listed and Non
- listed Species Chronic LOC only for mammals consuming short grasses (RQ = 1.08)
. Non
-target Pollinators None likely Lo w toxicity to bees.

### 9

**TABLE 5**  **. Summary of Environmental Risk Conclusions for Terrestrial Wildlife and Plants**  **Assessment Endpoint LOC**  **Exceedances Summarized Risk C**  **haracteri zation** 
Terrestrial Plants **Listed and Non**   ** listed Plant LOCs** **Non -**  **listed Plant Species RQ=**

The LOC wa s exceeded for non
- listed mo nocots and dico ts located in semi
- aquatic areas exposed adjacent to treatment for dicots (RQ

= 7.6 ) and for mo nocots (RQ = 2.29). For spray drift alone , the LOC was only exceeded for dicots (RQ = 1.21). No RQs exceeded the LOC for emergence of plants in adjacent dry areas (RQ = 0.27 for monocots; RQ = 0.9 for dicots)
. **Listed Plant Species RQ=** The LOC wa s excee ded for listed monocots and dicots located in semi
- aquatic areas exposed adjacent to treatment (RQ = 6.7 for mo nocots and 14.29 for dicots). For plants in adjacent dry areas, the LOC was exceeded only for dicots (RQ = 1.68). For spray drift alone , RQ for dicots exceed the LOC (RQ = 2.24 ), but no RQs for mo nocots (RQ = 0.28) exceed the LOC. **Additions:** Results of the AgDRIFT model show that the distance of the risk to listed plants via spray drift can range from 7 feet to 492 feet off the edge of treated s ite. That is, beyond 492 ft from the treated field, the RQ values are below

the LOC of 1. 

### 5. **SUMMARY**

**– LISTED SPECIES**  **(Direct and Indirect Effects)** Based on estimated maximum application rates, exposure levels and available effects data, amicarbazone used as an herbicide for control of certain annual grasses and annual broadleaf weeds and sedges on turf grass sites indicates direct effects LOC exceedances for birds, mammals, and terrestrial plants including alga . Therefore, there is a potential for indirect effects to listed animal and plant taxa that depend on those taxa directly at risk when exposed to amicarbazone as pollinators or seed dispersers, mammal or reptile burrows for habitat, feeding, or cover requirements, and for survival, growth, or reproduction . Listed species potentially at risk from direct or indirect effects from exposure to amicarbazone are presented in **Table 6** 
. **Table 6**  **. Potential listed species risks associated with direct or indirect effects due to the use of amicarbazone**  **on turf and conifers**  **. Listed Taxon**  **Direct Effects from Acute Exposures**  **Direct Effects from Chronic Exposures**  **Indirect Effects Aquatic**  Aquatic non
- vascular plants **Yes** N/A **Yes** Aquatic vascular plants No N/A **Yes** Freshwater invertebrates No No **Yes** Marine/estuarine invertebrates No No **Yes** Freshwater fish No No **Yes**

### 10

**Table 6**  **. Potential listed species risks associated with direct or indirect effects due to the use of amicarbazone**  **on turf and conifers**  **. Listed Taxon**  **Direct Effects from Acute Exposures**  **Direct Effects from Chronic Exposures**  **Indirect Effects** Marine/estuarine fish No No **Yes** Aquatic
-phase amphibians No No **Yes Terrestrial**  Semi
- aquatic plants
- monocots **Yes** N/A **Yes** Semi
- aquatic plants
- dicots **Yes** N/A **Yes** Terrestrial plants

– mo nocots No N/A **Yes** Terrestrial plants
- dicots **Yes** N/A **Yes** Insects No N/A **Yes** Birds No **Yes Yes**  Terrestrial
-phase amphibians No **Yes Yes**  Reptiles No **Yes Yes**  Mammals No **Yes Yes**  N/A
- indicates that this exposure route is not assessed.

### 11

**APPENDIX I. AMICARBAZONE TOXICITY PROFILE Appendix I**  **. Amicarbazone**  **Toxicity Profile for Aquatic Organisms**  **and Plants Species**  **(scientific name) Test Material**  **Endpoint Me**  **an -** **Measured Conc. (m** **g a.i./L) Study** 

**Classification Reference** 

**(M RID)**  **Freshwater Fish** Oncorhynchus mykiss (Rainbow trout) TGAI LC 50 **>120.4 A**  Supplemental 45121807 Lepomis macrochirus (Bluegill sunfish) TGAI LC 50 >128.5 Supplemental 45121804 Pimephales promelas (Fathead minnow) TGAI NOAEC **7.3** Acceptable 45121602 **Freshwater Invertebrates**  Daphnia magna (Water f lea) TGAI EC 50 **40.8** Acceptable 45121808

TGAI L OAEC **0.252** Supplemental 45121822 **Estuarine/marine Fish** Cyprinodon variegatus (Sheepshead minnow) TGAI LC 50 **>118** Acceptable 45121806 NOAEC **2.3 m g a.i./L**  Acceptable 48237510 **Estuarine/marine Invertebrates** Crassostrea gigas (Pacific oyster) TGAI EC 50 >63 Supplemental 45121809 Mysidopsis bahia (Mysid shrimp) TGAI LC 50 **109.7** Acceptable 45121810 TGAI NOAEC **5.0** Supplemental 45121601 **Aquatic Plants** Lemna gibba (Duckweed) TGAI EC 50 **0.21** Acceptable 45121805 NOAEC **0.0406** Navicula pelliculosa (Diatom) TGAI EC 50 0.1 Acceptable 45121812 NOAEC 0.098 Anabaena flos

- aquae (Blue
- green algae) TGAI EC 50 0.186 Acceptable 45121825 NOAEC 0.017 Skeletonema costatum (Marine diatom) TGAI EC 50 **0.017** Acceptable 45121818 NOAEC **0.0057** Selenastrum capricornutum (Green algae) TGAI EC 50 0.084 Acceptable 45121820 NOAEC 0.013 Deg of TGAI ( Desamino ) EC 50 >1.35 Acceptable 45121827

NOAEC 1.35

### 12

**Appendix I. Amicarbazone**  **Toxicity Profile for Terrestrial Wildlife**  **and Plants Species**  **(common na me)**  **Test Material Endpoint**  **Me an Concentration**  **( Endpoint affected)**  **Study Classification**  **Reference**

**(M RID)**  **WILDLIFE Upland Game**  **Birds and Waterfowls** 

Colinus virginianus (Northern bobw

hite quail) TGAI LD 50 **1965 m g a.i./kg bw**  Supplemental 45121811 LC 50 **>5690 m g a.i./kg diet**  Acceptable 48237511 NOAEC **27.1 m g a.i./kg diet** 

**(reduction in no. of viable embryos and %**  **hatchling per eggs set)** Acceptable 45121819 Deg of TGAI ( Desamino) NOAEC 1030 mg a.i./kg diet Acceptable 48237515 Deg of TGAI ( N
-Methyl Desamino) NOAEC 413 mg a.i./kg diet (hatchling and survivor body weights) Acceptable 48237514

Anas platyrhynchos (Mallard duck) TGAI LD 50 See bobwhite quail data N/A N/A LC 50 >10536 mg a.i./kg diet Acceptable 45121815 NOAEC 27.1 mg a.i./kg diet

(decreased eggshell thickness) Acceptable 45121814 Deg of TGAI ( Desamino) NOAEC 159 mg a.i./kg diet (numerous endpoints affected) Acceptable 48237512 Deg of TGAI ( N
-Methyl Desamino) NOAEC <161 mg a.i./kg diet (numerous endpoints affected) Supplemental 48237513 **Passerine Birds** No data TGAI LD 50 No data No data No data **Mam mals** 

Rattus norvegicus (laboratory rat) TGAI LD 50 **1087 m g a.i./kg bw**  Acceptable 45121605

NOAEC Systemic = 6.4/7.3

Reproductive = 73.2/84.0 Offspring = 6.4/7.3 mg/kg/day **[6.4 m g/kg/day**  **equivalent to 100 mg/kg diet]**  Acceptable 45121625

**Terrestrial Insects**  Apis mellifera (Honeybee) TGAI LD 50 **24.4 μg a.i./bee** Supplemental 45121816 **TERRESTRIAL PLANTS Seedling Emergence** 

### 13

Monocot; Onion TEP EC 25 **0.1 lb a.i./A** Acceptable 45121821 EC 0 5 1 **0.034 lb a.i./A** Dicot; Sugar beet (Beta vulgaris) EC 25 **0.03 lb a.i./A** NOAEC **0.016 lb a.i./A** Monocot; Wheat Deg of TEP

( N
-Methyl Desamino) EC 25 >0.418 lb a.i./A Acceptable 48237517

NOAEC 0.00493 lb a.i./A Dicot; Sugar beet (Beta vulgaris) EC 25 0.0866 lb a.i./A EC 0 5 1 0.00465 lb a.i./A Monocot; Ryegrass ( Lolium perenne) Deg of TEP

(Desamino) EC 25 >0.38 lb a.i./A Acceptable 48237516

NOAEC 0.0049 lb a.i./A Dicot; Cabbage (Brassica oleracea) EC 25 0.16 lb a.i./A NOAEC 0.061 lb a.i./A **Vegetative Vigor** Monocot: Onion TEP EC 25 **0.026 lb a.i./A** Acceptable 45121821 NOAEC **0.016 lb a.i./A** Dicot; Buckwheat EC 25 **0.0037 lb a.i./A** NOAEC **0.002 lb a.i./A** Monocot; Corn Deg of TEP

( N
-Methyl Desamino) EC 25 >0.457 lb a.i./A Acceptable 48237518

NOAEC 0.457 lb a.i./A Dicot; Sugar beet (Beta vulgaris) EC 25 0.0251 lb a.i./A NOAEC 0.00532 lb a.i./A Monocot; Wheat ( Triticum astivum ) Deg of TEP

(Desamino) EC 25 >0.30 lb a.i/A Acceptable 48237520

NOAEC 0.019 lb a.i./A Dicot; Sugar beet (Beta vulgaris) EC 25 0.318 lb a.i./A EC05* 0.122 lb a.i./A A Values in bold for each taxonomic groups are used in the risk assessment to assess acute and/or chronic risk to these groups 1 An EC05 is used when the NOAEC is below the lowest test concentration or above the EC25. * NOAEC was <0.0057 lb a.i./A

### 14

**APPENDIX II. AQUATIC RISK ASSESSMENT FOR TURF USES** 

**A. Freshwater Fish and Invertebrates**  **Table II**  **1. Tier II Risk Quotients for Freshwater Organisms for Proposed**  **Amicarbazone Use**  **on Turf**  **1, 2 Use**  **PRZM Scenario**  **Peak**

**EEC 21D**  **EEC 60D**  **EEC Freshwater Fish**  **(LC 50**  **>120.4 mg a.i./L ;**  **NOAEC = 7.3**  **mg a.i./L) Freshwater Invertebrate**  **(EC 50**  **= 40.8 mg a.i./L ;**  **L OAEC =**  **0.252 mg a.i./L**  ***** **) μg a.i./L**  **Acute 4**  **Chronic 5**  **Acute 6**  **Chronic 7**  Turf and Conifers FL nursery3 8.97 7.48 6.21 <0.01 <0.1 <0.01 0.0 3 1 LOC exceedances are bolded (Endangered Species LOC > 0.05; Acute Restricted LOC > 0.1; Acute Risk > 0.5 and Chronic LOC >1). 2 EECs from Section 2
. 3 Highest exposure scenario selected for new non
- crop uses (turf and conifers ) based on a suite of PR ZM/EXAMS scenarios modeled at 1 application at 0.044 lb a.i./A * The LOAEC was used as a surrogate for the NOAEC. The NOAEC was <0.252 mg/L, a non
- definitive endpoint. 4 RQ = peak EEC ÷ fish LC50 ; 5 RQ = 60
- Day EEC ÷ fish NOAEC ; 6 RQ = peak EEC ÷ invertebrate EC50 ; 7 RQ = 21
- Day EEC ÷ invertebrate NOAEC 

### B. **Estuarine/Marine Fish and Invertebrates Table**  **II2 . Tier II Risk Quotients for Estuarine/marine Organisms for Proposed**  **Am icarbazone**  **Use on Turf**  **1, 2 Use**  **PRZM**

**Scenario Peak** 

**EEC 21D**  **EEC 60D**  **EEC Marine/Estuarine Fish**  **(LC 50**  **> 118**  **mg a.i./L NOAEC =**  **2.3 mg a.i./L** **) Marine/Estuarine**  **Invertebrate (EC**  **50 = 10**  **9.7 mg a.i./L;**  **NOAEC = 5 mg a.i./L** **) μg a.i./L**  **Acute 4**  **Chronic 5**  **Acute 6**  **Chronic 7**  Turf and Conifers FL nursery3 8.97 7.48 6.21 <0.01 <0.1 < 0.01 <0.1 1 LOC exceedances are bolded (Endangered Species LOC > 0.05; Acute Restricted LOC > 0.1; Acute Risk > 0.5 and Chronic LOC >1). 2 EECs from Section 2
. 3 Highest exposure scenario selected for new non
- crop uses (turf and conifers ) based on a suite of PRZM/EXAMS scenarios modeled at 1 application of 0.44 lb a.i./A 4 RQ = peak EEC ÷ fish LC50 ; 5 RQ = 60
- Day EEC ÷ fish NOAEC ; 6 RQ = peak EEC ÷ invertebrate EC50 ; 7 RQ = 21
- Day EEC ÷ invertebrate NOAEC

### 15 **C. Aquatic Plants**  **Table II3**  **. Tier II Risk Quotients for Proposed Am** **icarbazone Use on**  **Turf 1,2**  **Use PRZM** 

**Scenario Peak EEC** 

**(μg a.i./L) Vascular Aquatic Plant** 

**(EC 50**  **= 210**  **μg a.i.** **/L NOAEC =**  **40.6 μg**  **a.i. /L)**  **Non -** **Vascular Aquatic Plant (EC**  **50 =**  **17 μg**  **a.i. /L;**  **NOAEC = 5.7**  **μg a.i.** **/L) Non**  **- target**  **4 Listed**  **5 Non**  **- target**  **6 Listed**  **7** Turf and Conifers FL nursery3 8.97 0.0 4 0.2 0. 5 **1. 6**  1 LOC exceedances are bolded (Non
- listed plant LOC > 1; Listed plant LOC > 1). 2 EECs from Section 2
. 3 Based on highest exposure scenario modeled for turf and conifers ( 1 app at 0.44 lb a.i./A) 4 RQ = peak EEC ÷ vascular EC50; 5 RQ = peak EEC ÷ vascular NOAEC; 6 RQ = peak EEC ÷ non
- vascular EC50; 7 RQ = peak EEC ÷ non - vascular NOAEC

### 16

**APPENDIX III**  **. PRZM/EXAMS OUTPUT**  **FO R TURF USE**  stored as FLnursery31.out Chemical: amicarbazone PRZM environment: FLnursery STD.txt modified Tueday, 26 August 2008 at 06:16:38 EXAMS environment: pond298.exv modified Tueday, 26 August 2008 at 06:14:08 Metfile: w12839.dvf modified Tueday, 26 August 2008 at 06:14:20 Water segment concentrations (ppb) Year Peak 96 hr 21 Day 60 Day 90 Day Yearl y 1961 1.963 1.938 1.844 1.64 1.405 0.5188 1962 1.71 1.692 1.622 1.491 1.396 0.5745 1963 0.344 0.341 0.328 0.3156 0.306 0.1529 1964 0.2912 0.2871 0.2732 0.2533 0.2475 0.09762 19 65 3.698 3.634 3.381 2.961 2.726 1.087 1966 0.5703 0.5626 0.5403 0.4792 0.4328 0.1585 1967 0.4355 0.4313 0.4141 0.3755 0.3337 0.1234 1968 0.2622 0.2596 0.2468 0.235 0.2109 0.06323 1969 0.5983 0.5932 0.5611 0.4992 0.4482 0.1659 1970 2.569 2.539 2.431 2.177 1.833 0.6754 1971 0.3862 0.3831 0.37 0.3412 0.3344 0.1621 1972 1.414 1.397 1.329 1.211 1.03 0.3462 1973 0.3733 0.3695 0.3507 0.313 0.2988 0.136 1974 2.924 2.888 2.718 2.382 2.162 0.8415 1975 0.4087 0.4051 0.3908 0.3588 0.3386 0.1831 1976 2.098 2.063 1.979 1.583 1.328 0.5076 1977 1.933 1.912 1.829 1.659 1.312 0.4042 1978 4.795 4.74 4.604 4.133 3.72 1.655 1979 4.787 4.227 3.297 2.649 2.305 1.018 1980 15.3 14.96 13.66 10.72 8.888 3.309 1981 0.6338 0.6291 0.6097 0.5819 0.5447 0.3006 1982 1.869 1.84 1.728 1.299 1.03 0.3424 1983 0.8761 0.8674 0.8344 0.7647 0.7004 0.2867 1984 11.91 11.53 10.47 8.44 6.853 2.293 1985 0.6998 0.6936 0.6664 0.6181 0.581 0.286 1986 9.432 9.084 7.799 6.441 5.527 1.999 1987 1.246 1.232 1.174 1.073 0.9882 0.4655 1988 0.4866 0.4779 0.4576 0.3863 0.3447 0.158 1989 0.2805 0.2779 0.2674 0.245 0.2284 0.09557 1990 0.3143 0.3073 0.2769 0.2538 0.2209 0.08319 Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearl y 0.032258064516129 15.3 14.96 13.66 10.72 8.888 3.309 0.0645161290322581 11.91 11 .53 10.47 8.44 6.853 2.293 0.0967741935483871 9.432 9.084 7.799 6.441 5.527 1.999

### 17

0.129032258064516 4.795 4.74 4.604 4.133 3.72 1.655 0.161290322580645 4.787 4.227 3.381 2.961 2.726 1.087 0.193548387096774 3.698 3.634 3.297 2.649 2.305 1.018 0.225806451612 903 2.924 2.888 2.718 2.382 2.162 0.8415 0.258064516129032 2.569 2.539 2.431 2.177 1.833 0.6754 0.290322580645161 2.098 2.063 1.979 1.659 1.405 0.5745 0.32258064516129 1.963 1.938 1.844 1.64 1.396 0.5188 0.354838709677419 1.933 1.912 1.829 1.583 1.328 0.50 76 0.387096774193548 1.869 1.84 1.728 1.491 1.312 0.4655 0.419354838709677 1.71 1.692 1.622 1.299 1.03 0.4042 0.451612903225806 1.414 1.397 1.329 1.211 1.03 0.3462 0.483870967741936 1.246 1.232 1.174 1.073 0.9882 0.3424 0.516129032258065 0.8761 0.8674 0.83 44 0.7647 0.7004 0.3006 0.548387096774194 0.6998 0.6936 0.6664 0.6181 0.581 0.2867 0.580645161290323 0.6338 0.6291 0.6097 0.5819 0.5447 0.286 0.612903225806452 0.5983 0.5932 0.5611 0.4992 0.4482 0.1831 0.645161290322581 0.5703 0.5626 0.5403 0.4792 0.4328 0 .1659 0.67741935483871 0.4866 0.4779 0.4576 0.3863 0.3447 0.1621 0.709677419354839 0.4355 0.4313 0.4141 0.3755 0.3386 0.1585 0.741935483870968 0.4087 0.4051 0.3908 0.3588 0.3344 0.158 0.774193548387097 0.3862 0.3831 0.37 0.3412 0.3337 0.1529 0.806451612903226 0.3733 0.3695 0.3507 0.3156 0.306 0.136 0.838709677419355 0.344 0.341 0.328 0.313 0.2988 0.1234 0.870967741935484 0.3143 0.3073 0.2769 0.2538 0.2475 0.09762 0.903225806451613 0.2912 0.2871 0.2732 0.2533 0.2284 0.09557 0.935483870967742 0. 2805 0.2779 0.2674 0.245 0.2209 0.08319 0.967741935483871 0.2622 0.2596 0.2468 0.235 0.2109 0.06323 0.1 8.9683 8.6496 7.4795 6.2102 5.3463 1.9646 Average of ye arl y averages: 0.616330333333333 Inputs generated b y pe5.pl
- Novemeber 2006 Data used fo r this run: Output File: FLnursery31 Metfile: w12839.dvf PRZM scenario: FLnurserySTD.txt EXAMS environment file: pond298.exv Chemical Name: amicarbazone Description Variable Name Value Units Comments Molecular weight mwt 241 g/mol Henry's Law Const. henry 1.5E
- 12 atm -m^3/mol Vapor Pressure vapr 2.2E
- 8 torr Solubility sol 4600 mg/L Kd Kd mg/L Koc Koc 16.7 mg/L

### 18

Photolysis half

- life kdp 0 days Half
- life Aerobic Aquatic Metabolism kbacw 140 days Halfife Anaerobic Aquatic Metabolism kbacs 0 days Halfife Aerobic Soil Metabolism asm 69.9 days Halfife Hydrolysis: pH 7 0 days Half
- life Method: CAM 1 integer See PRZM manual In corporation Depth: DEPI 0 cm Application Rate: TAPP 0.49 kg/ha Application Efficiency : APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of a pplication rate applied to pond Application Date Date 01
- 03 dd/mm or dd/mmm or dd
-mm or dd
-mmm Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR EPA Pond Flag for runoff calc. RUNOFF none, monthly or total(average of entire run)

### 19



## APPENDIX **I V.**  **T -** **REX v. 1.4.1**  **(Terrestrial Exposure Model for Birds and Mammals) Input and Output**  **Corn /Turf**  **Use –**  **Upper Bound (1 x 0.45 lb ai/A x 35D Half** **- life)**  **Upper Bound Kenaga Residues For RQ Calculation**

**Chemical Name: Am**  **icarbazone**

**Use: Corn**  **Formulation: Ap**  **plication Rate: 0.4** **484 lbs ai/A** 

**Half -**  **life: 35 day** **s Ap**  **plication Interval: N/A**  **Maximum # A pps./Year:**  **1 application Length of Simulation:**  **1 y ear**  

### Endpoints
**Av ian**  **Quail LD50 (mg/kg**  **- bw)**  **1965 Quail**  **LC50 (mg/kg**  **- diet)**  **>5690**

**NOA EL(mg/kg**  **- bw)**  **0.00 Quail**  **NOA EC (mg/kg**  **- diet)**  **27.1**
**Ma mmals**  **Rat LD50 (mg/kg**  **- bw)**  **1087 LC50 (mg/kg**  **- diet)**  **0.00 Rat**  **NOA EL (mg/kg**  **- bw)**  **6.4 Rat**  **NOA EC (mg/kg**  **- diet)**  **100 Dietary**  **- Based EECs** 

**Food Items Upper Bound**  **EEC (mg ai/kg)**  **Me an EEC** 

**(mg ai/kg) Short Grass**  **Tall Grass Sm. Insects, Broadleaf Plants**  **Lg. Insects, Fruits, Pods 107.62**  **49.32 60.53**  **6.73 8.11** 

**16.14 20.18**  **3.14**


## AV **IA N EECs and ADJUSTED LD50s** 
**Avian Class Body** 

**Weight (g)**  **Ingestion**

**(Fdry )**  **(g b w/day** **) Ingestion** 

**(Fwet) (g/day** **) % body** 

**w gt**  **consumed FI** 

**(kg -**  **diet/day) Small**  **20 5**  **23 114**  **2.28E -**  **02 Mi**  **d 100**  **13 65**  **65 6.49E**  **- 02**  **Large 1000**  **58 291**  **29 2.91E**  **- 01** 

### 20

**Avian Body Weight (g)**  **Ad justed LD50**  **(mg/kg -**  **bw )**  **20 1415.64**  **100 1802.19**  **1000 2545.66**  

## Dose **- based EECs**  **(mg/kg -**  **bw )** 

**Food items Av**  **ian Classes and Body Weights small**  **mid large**  **20 g 100 g**  **1000 g Upper Bound**  **EEC (mg/kg) Short Grass**  **Tall Grass Sm. Insects, Broadleaf Plants**  **Lg. Insects, Fruits, Pods 122.56**  **69.89 31.29**  **56.18 32.03**  **14.34 68.94**  **39.31 17.60**  **7.66 4.37**  **1.96 Me**  **an EEC (mg/kg) Short Grass**  **Tall Grass Sm. Insects, Broadleaf Plants**  **Lg. Insects, Fruits, Pods 43.41**  **24.75 11.08**  **18.38 10.48**  **4.69 22.98**  **13.10 5.87**  **3.57 2.04**  **0.91** 

## MA**MMA LI**  **AN EECs and A DJUSTED LD50s** 
**Mammalian Class**  **Body**

**Weight Ingestion** 

**(Fdry )**  **(g b wt** **/day )**  **Ingestion**

**(Fwet) (g/day** **) % body** 

**w gt**  **consumed FI** 

**(kg -**  **diet/day) Herbivores/**  **insectivores 15**  **3 14**  **95 1.43E**  **- 02**  **35 5**  **23 66**  **2.31E -**  **02 1000**  **31 153**  **15 1.53E**  **- 01**  **Granivores 15**  **3 3**  **21 3.18E**  **- 03**  **35 5**  **5 15**  **5.13E -**  **03 1000**  **31 34**  **3 3.40E**  **- 02** 

**Mammalian Class**  **Body Weight**  **Ad justed**  **LD50 Ad** **justed NOA**  **EL Herbivores/**  **insectivores 15**  **2389.04 14.07**  **35 1932.99**  **11.38 1000**  **836.08 4.92**  **Granivores 15**  **2389.04 14.07**  **35 1932.99**  **11.38 1000**  **836.08 4.92** 

### 21



## Dose **- based EECs**  **(mg/kg -**  **bw) Mammalian**  **Class es and Body**  **Weight Herbivores and Insectivores**  **Gra n**  **i vores**  **15 g 35 g**  **1000 g 15 g**  **35 g 1000 g**  **Upper Bound EECs (mg ai/kg) Short Grass**  **102.60 70.91**  **16.44 Tall Grass**  **47.03 32.50**  **7.54 Broadleaf plants and small insects**  **57.71 39.89**  **9.25 Fruits/pods/seeds/large insects**  **6.41 4.43**  **1.03 1.43**  **0.98 0.23**  **Me an EECs (mg ai/kg)**  **Short Grass 36.34**  **25.11 5.82**  **Tall Grass 15.39**  **10.64 2.47**  **Broadleaf plants and small insects 19.24**  **13.30 3.08**  **Fruits/pods/seeds/large insects**  **2.99 2.07**  **0.48 0.67**  **0.46 0.11**  

## RESULTS

**– Upper Bound EECs and RQs** 
**Table I** **V 1**  **. Upper Bound**  **Kenaga, Acute Avian Dose -**  **Based Risk Quotients**
**Size Class (gram** **s) Adjusted** 

**LD50 EECs and RQs** 

**Short Grass Tall Grass**  **Broadleaf Plants/**  **Sm all Insects**  **Fruits/Pods/**

**Seeds/ Large Insects**  **EEC RQ**  **EEC RQ**  **EEC RQ**  **EEC RQ**  20 1415.64 122.56 0.09 56.18 0.04 68.94 0.05 7.66 0.01 100 1802.19 69.89 0.04 32.03 0.02 39.31 0.02 4.37 0.00 1000 2545.66 31.29 0.01 14.34 0.01 17.60 0.01 1.96 0.00 Bold value indicates LOC exceedance **Table I** **V 2**  **. Upper Bound**  **Kenaga, Subacute Avian Dietary Based Risk Quotients**

**LC50 EECs and RQs** 

**Short Grass Tall Grass**  **Broadleaf Plants/**  **Sm all Insects**  **Fruits/Pods/**

**Seeds/ Large Insects**  **EEC RQ**  **EEC RQ**  **EEC RQ**  **EEC RQ**  >5690 107.62 <0.02 49.32 <0.01 60.53 <0.01 6.73 <0.01 Size class not used for dietary risk quotients Bold value indicates LOC exceedance **Table IV** **3 . Upper**  **Bound Kenaga, Chronic Avian Dietary Based Risk Quotients** 

**NOAEC (**  **mg /k** **g -**  **diet )**  **EECs and RQs**

**Short Grass Tall Grass**  **Broadleaf Plants/**  **Sm all Insects**  **Fruits/Pods/**

**Seeds/ Large Insects**  **EEC RQ**  **EEC RQ**  **EEC RQ**  **EEC RQ**  27 107.62 **3.97** 49.32 **1.82** 60.53 **2.23** 6.73 0.25 Size class not used for dietary risk quotients Bold values indicate LOC exceedances

### 22

**Table IV** **4 . Upper**  **Bound Kenaga, Acute Mammalian Dose**  **- Based Risk Quotients** 
**Size Class (gram** **s) Adjusted** 

**LD50 EECs and RQs** 

**Short Grass Tall Grass**  **Broadleaf Plants/**  **Sm all Insects**  **Fruits/Pods/**

**Seeds/ Large Insects**  **Granivores EEC**  **RQ EEC**  **RQ EEC**  **RQ EEC**  **RQ EEC**  **RQ** 15 2389.04 102.60 0.04 47.03 0.02 57.71 0.02 6.41 0.00 1.43 0.00 35 1932.99 70.91 0.04 32.50 0.02 39.89 0.02 4.43 0.00 0.98 0.00 1000 836.08 16.44 0.02 7.54 0.01 9.25 0.01 1.03 0.00 0.23 0.00

**Table IV** **5 . Upper**  **Bound Kenaga, Chronic Mamm**  **alian Dietary Based Risk Quotients NOAEC**  **( mg** **/k g**  **- diet** **) EECs and RQs** 

**Short Grass Tall Grass**  **Broadleaf Plants/ Sm** **all Insects Fruits/Pods/**  **Seeds/ Large Insects**  **EEC RQ**  **EEC RQ**  **EEC RQ**  **EEC RQ**  100 107.62 **1.08** 49.32 0.49 60.53 0.61 6.73 0.07 Size class not used for dietary risk quotients
**Table IV**  **6 . Upper**  **Bound Kenaga, Chronic Mamm**  **alian Dose -**  **Based Risk Quotients**
**Size Class (gram** **s) Adjusted** 

**NOAEL EECs and RQs** 

**Short Grass Tall Grass**  **Broadleaf Plants/**  **Sm all Insects**  **Fruits/Pods/**

**Seeds/ Large Insects**  **Granivores EEC**  **RQ EEC**  **RQ EEC**  **RQ EEC**  **RQ EEC**  **RQ** 15 14.07 102.60 **7.29** 47.03 **3.34** 57.71 **4.10** 6.41 0.46 1.43 0.10 35 11.38 70.91 **6.23** 32.50 **2.86** 39.89 **3.50** 4.43 0.39 0.98 0.09 1000 4.92 16.44 **3.34** 7.54 **1.53** 9.25 **1.88** 1.03 0.21 0.23 0.05 Bold values indicate LOC exceedances

### 23



## Appendix **V . Terrestrial Plants Exposure Modeling and Results (TerrPlant v**  **1.2.2) 1. Ground Application of 0.4484 lbs ai/A to Corn/Turf**  **Table V**  **1. Chemical Identity.**  **Chemical Name Am**  **icarbazone PC code**  **114004 Use**  **Corn/Turf Ap**  **plication Method Ground**  **Ap plication Form**  **liquid Solubility in Water (ppm)**  **4,600 Table**  **V 2. Input parameters used to derive EECs.**  **Input Parameter Symbol**  **Value Units**  Application Rate A **0. 4484**  **lbs ai/A** Incorporation I **1** none Runoff Fraction R **0.05** none Drift Fraction D **0.01** none **Table V** **3. EECs for Flutriafol 125 g/l SC. Units in lbs ai/A .** 

**Description Equation**  **EEC** Runoff to dry

areas (A/I)*R 0.02242 Runoff to semi - aquatic areas (A/I)*R*10 0.2242 **Spray drift** **A*D** **0.004484 Total for dry areas**  **((A** **/I)*R)+(** **A*D)** **0.026904 Total for semi**  **- aquatic areas**  **((A** **/I)*R*10)+(** **A*D)** **0.228684 Table**  **V 4. Plant survival and**  **growth data used for RQ derivation. Units are in lbs ai/A .**  **Seedling Emergence Vegetative Vigor**  **Plant type EC**  **25 NOA**  **EC EC**  **25 NOA**  **EC** Monocot **0.1 0.034**  **0.026 0.016**  Dicot **0.03 0.016**  **0.0037 0.002**  **Table V** **5. RQ values for plants in dry and semi**  **- aquatic areas exposed to Flutriafol 125 g/l SC through**  **runoff and/or spray drift.***

**Plant Type Listed Status**  **Dry Semi**  **- Aq**  **uatic Spray Drift**  Monocot non - listed 0.27 **2.29** 0.17 Monocot listed 0.79 **6.73** 0.28 Dicot non - listed 0.90 **7.62 1.21**  Dicot listed **1.68 14.29**  **2.24** *If RQ > 1.0, the LOC is exceeded (bolded) , resulting in potential for risk to that plant group. INC - inconclusive

### 24

**APPENDIX VI**  **. DATA EVALUATION RECORDS AND STUDY CLASSIFICATION**  **OF NEWLY SUBMITTED DATA**  **OPPTS Guideline**  **No. Test** 

**Material MRID**  **Study Classification**  **Comments** 850.1400 TGAI
- Parent 48237510 Acceptable None 850.2200 TGAI
- Parent 48237511 Acceptable None 850.2300 Deg of TGAI
Desamino 48237512 Acceptable None 850.2300 Deg of TGAI
N
- methyl 48237513 Supplemental NOAEC is below lowest dose. A new study with lower doses is needed. 850.2300 Deg of TGAI
N
- methyl 48237514 Acceptable None 850.2300 Deg of TGAI
Desamino 48237515 Acceptable None 850.4100 Deg of TEP
Desamino 48237516 Acceptable None 850.4100 Deg of TEP
N
- methyl 48237517 Acceptable None 850.4150 Deg of TEP
N
- methyl 48237518 Acceptable None 850.4150 TEP
- Parent 48237519 Acceptable Tomato and sugar beet were repeated to satisfy MRID 45121821 850.4150 Deg of TEP
-

Desamino 48237520 Acceptable None

### 25