1

OFFICE OF

CHEMICAL SAFETY AND 

POLLUTION PREVENTION 

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY

WASHINGTON D.C., 20460

PC Code: 114004

DP Barcodes: D384516

Date: August 30, 2010

MEMORANDUM 

 

SUBJECT: Section 3 Registration for New Use of Amicarbazone on Turf Grasses Grown 

Nationwide.

TO: Kathryn Montague, Risk Manager (RM 23)

Michael Walsh, Team Reviewer

Product Registration Division (7505P) 

FROM: Stephen Carey, Biologist

Andrew Shelby, Physical Scientist

Environmental Risk Branch 6

Environmental Fate and Effects Division (7507P) 

THROUGH: Mark Corbin, Branch Chief

Environmental Risk Branch 6

Environmental Fate and Effects Division (7507P)

This memorandum transmits the Section 3 Ecological and Environmental Risk Conclusions, 

based on the Environmental Fate and Ecotoxicity Assessments for the proposed new use on 

turfgrasses to the labeling for Amicarbazone DF Herbicide (EPA Reg. No. 66330-46) to allow its 

use on golf courses, sod farm, school grounds, commercial and recreational turf grass, including 

residential turf grass and other turf areas and conifers in nurseries and field plantings, including 

Christmas trees. The maximum proposed annual application rate at 0.44 lb a.i./A per year is 

slightly lower than the current maximum annual rate, registered for application to field corn and 

corn grown for silage at 0.45 lb a.i./A per year. Additionally, the registrant has submitted 

conditional ecological effects data for the active ingredient on chronic toxicity to 

estuarine/marine fish (MRID 48237510) and acute dietary to bobwhite quail (MRID 48237511); 

data for the metabolites of amicarbazone (both desamino and N-methyl desamino) on bobwhite 

quail (MRIDs 48237514 & 48237515) and mallard reproduction (MRIDs 48237512 & 

48237513) and on the emergence (MRID 48237516 & 48237517) and vigor (MRIDs 48237518, 

48237519 & 48237520) of terrestrial plants required for registration. The study classification and 

results of the newly submitted data, including the data evaluation reports (DERs) are attached in 
2

Appendix VI. Ecological endpoints have not changed with the additional data except for 

acceptable endpoints for chronic toxicity to estuarine/marine fish and dietary toxicity to 

bobwhite quail, which did not impact the risk conclusions. Also, data on the metabolites suggests 

they are less toxic than the parent compound; thus, all ecological endpoints used in the 

assessment remain the same with the parent compound as the most toxic material which does not 

alter risk conclusions from the previous assessment. In that assessment, amicarbazone was found 

to present potential chronic risk to birds and mammals. There is also the potential for risk to 

both terrestrial and aquatic plants. Minimal risk was found for fish and aquatic invertebrates. 

Further characterization can be found in the New Chemical assessment (DP Barcode D288631; 

USEPA 2005).

1. SUMMARY – USE CHARACTERIZATION 

Amicarbazone belongs to the triazolinone class of compounds and is a photosystem II (PS II) 

inhibitor with burn-down activity. This portion of the photosynthesis cycle uses light energy to 

drive two chemical reactions: the oxidation of water and the reduction of plastoquinone. No 

specific information is available on the mechanism of action of amicarbazone which affects the 

PS II process. Current/proposal label language indicates that this herbicide is not to be used on

coarse-textured soils (sand, loamy sand and sandy loam soils), or on soils with pH >7.4, such as 

calcareous soils. Amicarbazone is proposed to be applied as broadcast ground spray or via spot 

treatment using backpack sprayers at maximum rates of 1.0 -10.0 oz formulated 

product/acre/year (0.044 - 0.44 lb a.i/acre/year). The shortest reapplication interval is 7 days. A 

nonionic surfactant with at least 80% a.i. should be added at 0.25% v/v to the spray mix. 

Amicarbazone can be used as part of tank-mixes to control emerged weeds. The proposed 

maximum single application rate for turf is slightly less than the maximum single rate for 

currently registered corn uses. Corn uses of amicarbazone currently registered are included in 

Table 1 for comparison. More information on the use characterization for corn can be found in 

D288631 (USEPA 2005).

Table 1. Summary of Current and Proposed Uses of Amicarbazone

USE MAX SINGLE 

APP. RATE 

(lbs. a.i./A)

NUMBER OF 

APPS.

SEASONAL 

APP. RATE

(lbs. a.i./A)

APPLICATION

METHOD

Proposed new use:

Turf grasses and Conifers 0.44 1 0.44 Ground Spray

Current uses:

Corn 0.45 1 (full) or 2 

(split)

0.45 Ground Spray

2. SUMMARY – ENVIRONMENTAL FATE 

Amicarbazone is moderately persistent in the environment as it eventually transforms by biotic 

mechanisms (t1⁄2= 87 days; aerobic soil metabolism). Under acidic and neutral environments, 

amicarbazone is stable to hydrolysis, but transforms slowly under alkaline conditions. Photolysis 
3

also occurs slowly for amicarbazone in water and on soil with half-lives on the order of about 

two months, estimated for outdoor environmental conditions. The primary transformation 

products of amicarbazone are Des-amino: N-(1,1-dimethylethyl)-4,5-dihydro-3-(1-methylethyl)-

5- oxo-1H-1,2,4-triazole-1-carboxamide; N-methyl Des-amino: N-(1,1-dimethylethyl)-4,5-

dihydro-4-methyl-3-(1-methylethyl)-5-oxo-1H-1,2,4-triazole-carboxamide; and 

Decarboxamide: 4-amino-2,4-dihydro-5-(1-methylethyl)-3H-1,2,4-triazol-3-one. 

Amicarbazone has a low vapor pressure and Henry’s Law constant; therefore, volatilization from 

water and soil surfaces is not expected to be an important environmental fate process. The 

moderate persistence and high mobility of amicarbazone in soil make leaching of this compound 

to lower soil horizons and groundwater possible. Likewise, leaching and possible ground water 

contamination with amicarbazone’s major three metabolites are possible due to their apparent 

long persistence and high mobility. Small scale prospective groundwater studies have been 

requested and submitted to the Agency. 

Amicarbazone is not to be applied on soils with pH values higher than 7.4. Therefore, the fate of 

the chemical under neutral and acidic conditions only is considered in this assessment. In such 

environments, two major amicarbazone transformation products (Des-amino and N-methyl Desamino) are expected to be formed. In metabolism studies, these metabolites increase in 

concentration to the end of study. Given the moderate persistence/high mobility and solubility of 

the parent compound and the apparent high persistence/high mobility of the two transformation 

products, both amicarbazone and its first two transformation products are expected to dissipate 

slowly and at the same time be vulnerable to leaching/run-off. Hydrolysis studies on the desamino and N-methyl des-amino metabolites indicate stability at environmental relevant 

temperatures and pH (MRIDs 48073601 and 48237506). If amicarbazone is transported into 

alkaline ground water, surface water bodies and/or soils, the third transformation product 

(Decarboxamide) may be formed since this was the only major metabolite identified in the basecatalyzed hydrolytic transformation of amicarbazone. 

Parent amicarbazone only is considered in this assessment due to: 1) a freshwater green algae 

study indicating the des-amino metabolite is less toxic than parent; 2) four avian reproduction 

studies indicating the metabolites, des-amino and N-methyl, are less toxic than the parent; and 3) 

seedling emergence and vegetative vigor studies indicating the metabolites, des-amino and Nmethyl, are less toxic than the parent. To represent new uses on turf and conifers across the 

conterminous United States, nine PRZM scenarios were utilized with the Florida Nursery

scenario resulting in the highest EECs of all scenarios. 

Table 2. PRZM/EXAMS input parameter values for amicarbazone use on turf and conifers

PARAMETER (units) VALUE(S) SOURCE COMMENT

Application Rate (kg a.i./ha) Parent: 0.490 Product 

label; 

Represents the maximum possible single 

application rate per crop season (year) for 

all new uses. 
4

Table 2. PRZM/EXAMS input parameter values for amicarbazone use on turf and conifers

PARAMETER (units) VALUE(S) SOURCE COMMENT

Number of Applications 1 Maximum single application rate equals 

maximum annual application. Multiple 

applications at lower rates are possible only 

a single application can be done at the 

maximum rate

Molecular weight (g/mol) Parent: 241 Product 

Chemistry

-

Henry’s Law Constant (atmm

3

/mol)

1.5 x 10-12 (Vapor Pressure [torr]/760)/(Solubility 

[mg/L]/Molecular Weight [g/mol])

Vapor Pressure (torr) 2.2 x 10-8-

Solubility in Water @ 20 OC, 

(mg/L or ppm)

4,600 Product 

Chemistry

–

Organic Carbon Partition 

Coefficient (mL/g)

16.7 45121719; 

45121732; 

45121801; 

45121733

KOC ranges from 16.7 to 37.0. Lowest nonsand KOC used for modeling. KOC is 

correlated to KD (r2= 0.62).

CAM (Chemical Application 

Method)

1 ground spray; pre-emergence, 

unincorporated

Depth of Incorporation (inches) 0 Product 

label

–

Application efficiency (decimal) 0.99

Spray drift fraction (decimal) 0.01

Application date March 1 Spring applications are stipulated on the 

label for turf and conifers. Two spring 

applications were modeled. The indicated 

date resulted in highest EECs

Hydrolysis Half-life (days) Stable 45121730 

Aqueous Photolysis Half-life @ 

pH 7 (days)

Stable 45121803

Aerobic Aquatic Metabolism Halflife (days)140 2x Aerobic Soil Metabolism half-life

Anaerobic Aquatic Metabolism 

Half-life (days)

Stable 45121724

Aerobic Soil Metabolism Half-life 

(days)

69.9 45121731; 

48237507

90th percentile average of 5 half-life 

estimates: 36.7 days, 40.3 days, 42.8 days, 

68 days and 87 days
5

Estimated environmental concentrations (EECs) resulting from the above input parameters are 

can be found in the following table. Results from the Florida Nursery scenario are used for risk 

estimation with EECs of 8.97 ppb (peak), 7.48 ppb (21-day exposure), and 6.21 ppb (60-day

exposure). The previous assessment for use of amicarbazone on corn resulted in EECs of 33.78 

ppb (peak), 33.53 ppb (21-day exposure), and 29.14 ppb (60-day exposure). PRZM/EXAMS 

output can be found attached in Appendix III.

Table 3. PRZM/EXAMS EECs for amicarbazone use on turf and conifers

Scenario Application 

date

Peak (ppb) 21-day exposure 

(ppb)

60-day exposure 

(ppb)

CA Nursery February 15 6.32 6.10 5.78

March 1 3.76 3.58 3.33

FL Turf February 15 0.47 0.46 0.43

March 1 0.49 0.46 0.42

FL Nursery February 15 5.17 4.70 4.29

March 1 8.97 7.48 6.21

MI Nursery February 15 2.67 2.56 2.30

March 1 3.84 3.61 3.30

NJ Nursery February 15 4.79 4.58 4.38

March 1 5.07 4.94 4.67

OR Christmas 

tree

February 15 1.11 1.07 1.00

March 1 0.80 0.78 0.75

OR Nursery February 15 1.99 1.94 1.84

March 1 1.68 1.64 1.57

PA Turf February 15 0.58 0.58 0.56

March 1 0.81 0.79 0.77

TN Nursery February 15 5.59 5.34 4.42

March 1 5.91 5.69 4.86

3. SUMMARY – ECOLOGICAL EFFECTS

Results of acute toxicity studies indicate that technical grade amicarbazone (amicarbazone 

TGAI) is practically non-toxic to honey bees, slightly toxic to mammals, and slightly toxic to 

practically non-toxic to birds. New data on the acute dietary with bobwhite quail indicates 

amicarbazone is practically non-toxic to birds (LC50 >5690 mg a.i./kg diet). However, results of 

chronic toxicity studies show that longer-term exposure to amicarbazone TGAI and the two 

metabolites, desamino amicarbazone and N-methyl desamino amicarbazone, result in adverse 

effects on growth and reproductive parameters in birds. In addition, exposing the TGAI to 

parents and offspring in mammals resulted in reduced growth parameters. No data are available 

to characterize the effects of amicarbazone metabolites in mammals or insects; however, the bird 

reproduction studies indicate the metabolites are 6 to 38 times less toxic than the parent 

compound. No data with passerine birds are available; an acute oral passerine bird study is 

required to satisfy EPA guideline requirements. 
6

Results of Tier 2 seedling emergence and vegetative vigor terrestrial plant studies with the 

amicarbazone formulated product MKH 3586 70 WG show that both monocots and dicots are 

sensitive to exposure, with dicots appearing more sensitive. Tier 2 seedling emergence and 

vegetative vigor studies with the amicarbazone metabolites (Des-amino and N-methyl) exhibited 

some effects; however, both are approximately 2-fold less toxic than the parent compound. 

Toxicity studies demonstrate that amicarbazone TGAI is practically non-toxic on an acute basis 

to freshwater and estuarine/marine fish, slightly toxic to freshwater invertebrates, and slightly 

toxic to practically non-toxic to estuarine/marine invertebrates. Longer-term exposure of 

aquatic animals to amicarbazone produces adverse effects on growth parameters in freshwater 

fish and on growth and reproductive parameters in freshwater and estuarine/marine invertebrates. 

New data indicates longer-term exposure of estuarine/marine fish to amicarbazone resulted in no 

effects as high as 2.3 mg a.i./L. No data are available to characterize the effects of amicarbazone 

metabolites in aquatic animals but with the parent persistent and stable to hydrolysis, the 

metabolites are expected to have a minimal impact on aquatic animals in acidic or neutral surface 

waters. However, the alkaline hydrolysis metabolite, Decarboxamide, may be formed in alkaline 

surface waters. No data for the alkaline metabolite, Decarboxamide, is available; however, 

because current/proposal label language prohibit applying on coarse-textured soils (sand, loamy

sand and sandy loam soils), or on soils with pH >7.4, such as calcareous soils; data on the effect 

of the alkaline metabolite to aquatic organisms is not required at this time.

In the aquatic environment, amicarbazone appears to be particularly toxic to algae, but produces 

adverse effects on survival and growth in both algae and vascular plants. The results of a single 

study assessing the toxicity of the amicarbazone metabolite Des-amino in Selenastrum 

capricornutum (a freshwater green algae), indicate that Des-amino is 16-fold less toxic than the 

parent compound.

An updated toxicity profile for amicarbazone can be found in Appendix I.

4. SUMMARY – RISK CONCLUSIONS

The ecological assessment for amicarbazone as a new chemical has a slightly higher application 

rate, 0.45 lb a.i./A than the proposed new use: a maximum single application rate of 0.44 lb 

a.i./A, applied as a ground spray. The crop scenarios for turf and conifers lead to slightly lower

exposure estimates, but the change is not significant enough to alter previous risk conclusions. 

Environmental exposure inputs and ecological endpoints have not changed significantly, so the 

conclusions from the New Chemical assessment remain relevant for the current proposal (DP 

Barcode D288631). A complete endangered species analysis for these conclusions is included in 

that document as well; a summary of the expected effects to listed species is provided below.

4.1. Risk to Aquatic Organisms and Plants

The previous assessment as a New Chemical for amicarbazone concluded that there was

negligible risk to aquatic organisms and vascular plants (duckweed) except for algae at a Tier II 

level, where exposure estimates are dependent on crop specific scenarios as well as on 

application rates. Additionally, newly submitted data with estuarine/marine fish indicate chronic 
7

risks to these organisms are expected to below the levels of concern (Appendix II). Given 

negligible changes in the risk picture, the previous risk conclusions can still apply to the 

proposed new use, as presented in Table 4. The results of the new risk quotients (RQs) for 

aquatic organisms using scenarios for turf and conifers are lower than corn uses but do not alter

the risk conclusions and can be found in Appendix II. 

TABLE 4. Summary of Environmental Risk Conclusions for Aquatic Organisms and Plants

Assessment Endpoint LOC 

Exceedances

Summarized Risk Characterization

Acute Risk to 

Freshwater & 

Estuarine/Marine

Fish and Invertebrates

None Acute RQs =

At the peak EECs, there are no exceedances of the Non-listed 

Species Acute Risk, Acute Restricted Use, or Listed Species Acute 

Risk LOCs for freshwater and estuarine/marine fish or invertebrates.

The acute toxicity values [ranging from 40,000 to 120,000 μg/L] are 

at least three orders of magnitude higher than the peak EEC.

Chronic Risk to

Freshwater 

Fish and Invertebrates

None Chronic RQs = 

There are no Listed and Non-listed Chronic LOC exceedances for 

freshwater fish or invertebrates. The highest 21- and 60-day EECs 

in surface water are at least two orders of magnitude lower than the 

lowest toxicity values, for rainbow trout (NOAEC of 7300 μg/L) and 

daphnids (LOAEC of 252 μg/L). 

Uncertainty: 

An uncertainty exists for chronic exposure of the TGAI to freshwater 

invertebrates because effects were observed at the lowest 

concentration in the test; thus, the non-definitive NOAEC could not 

be used to assess risk to freshwater invertebrates. A closer look 

shows that a NOAEC would need to be 7.48 μg a.i./L or 34 times 

lower to yield an RQ that exceeds the Listed and Non-listed Chronic 

LOC of 1. 

Chronic Risk to 

Estuarine/Marine 

Fish and Invertebrates

None Chronic RQs =

There are no Listed and Non-listed Chronic LOC exceedances for 

freshwater fish or invertebrates. The highest 21- and 60-day EECs 

in surface water are at least two orders of magnitude lower than the 

lowest toxicity values, for Sheepshead minnow (NOAEC of 2300 

μg/L) and mysids (NOAEC of 5000 μg/L). 

Risk to Aquatic Vascular

Plants

None Vascular Plant RQs = 

At the peak EEC, there are no exceedances of the Non-Listed Plant 

or Listed Plant LOCs for vascular plants inhabiting water bodies. 

The non-listed plant and listed plant toxicity values [210 to 40.6 

μg/L] average one order of magnitude higher than the highest peak 

EECs.

Risk to Aquatic NonVascular Plants

Listed Plant Non-Vascular Plant RQs = 

There is an exceedance of the listed plant LOC for non-vascular 

plants with RQs as high as 1.6. The non-listed plant LOC is not 

exceeded (RQ = 0.5).
8

4.2. Risk to Terrestrial Wildlife and Plants

For terrestrial plants and animals, risk conclusions are not crop dependent and so the conclusions 

for the corn use, summarized in Table 5 below, also apply to the proposed new use. Details of 

the risk conclusions and its calculations for the corn use risk assessment on birds, mammals and 

plants based on a maximum single application rate of 0.4484 lb a.i./A can be found in Appendix 

IV for birds and mammals and Appendix V for plants.

TABLE 5. Summary of Environmental Risk Conclusions for Terrestrial Wildlife and Plants

Assessment Endpoint LOC 

Exceedances

Summarized Risk Characterization

Acute Risk to 

Birds 

None Acute RQs =

The acute RQs range <0.01 to <0.088 for all weight classes of birds 

consuming all feed types were less than the Listed and Non-listed 

Acute LOCs, indicating risk adverse effects is minimal.

Using the dietary-based LC50, no Listed and Non-listed Species 

Acute LOCs were exceeded (RQs range <0.001 to <0.01) .

Chronic Risk to 

Birds 

Listed and Nonlisted Species 

Chronic LOC

Chronic RQs = 

Using the dietary-based NOAEC, the Listed and Non-listed Species

Chronic LOC was exceeded for birds consuming short grasses at 

the maximum application rate of 0.45 lb a.i./A (RQ = 3.97), 

broadleaf plants/small insects (RQ = 2.23), and tall grass (RQ = 

1.82) and maximum predicted residues. No risks were observed 

for birds feeding on fruit, pods, seeds, large insects (RQ = 0.25).

Acute Risk to 

Mammals 

None Acute RQs =

The acute RQs range <0.001 to <0.043 for all weight classes of 

mammals consuming all feed types were less than the Listed and 

Non-listed Acute LOCs, indicating risk adverse effects is minimal.

Chronic Risk to 

Mammals

Listed and Nonlisted Species 

and Chronic 

LOCs

Chronic RQs =

At maximum predicted residue levels and maximum application 

rate of 0.45 lb a.i./A, the dose-based RQs exceeded the Listed and 

Non-listed Species Chronic LOC for all weight classes of mammals 

(15, 35, 1000 g) consuming short grasses (RQs range 3.28 to 7.27),

tall grasses (RQs range 1.5 – 3.33) and broadleaf forage/small 

insects (RQs range 1.84 to 4.09). No risks were observed for 

mammals feeding on fruits, pods, seeds, large insects (RQs range 

0.05 – 0.45).

Dietary-based RQs exceed the Listed and Non-listed Species 

Chronic LOC only for mammals consuming short grasses (RQ = 

1.08).

Non-target Pollinators None likely Low toxicity to bees. 
9

TABLE 5. Summary of Environmental Risk Conclusions for Terrestrial Wildlife and Plants

Assessment Endpoint LOC 

Exceedances

Summarized Risk Characterization

Terrestrial Plants Listed and Nonlisted Plant LOCs

Non-listed Plant Species RQ=

The LOC was exceeded for non-listed monocots and dicots located

in semi-aquatic areas exposed adjacent to treatment for dicots (RQ

= 7.6) and for monocots (RQ = 2.29). For spray drift alone, the 

LOC was only exceeded for dicots (RQ = 1.21). No RQs exceeded 

the LOC for emergence of plants in adjacent dry areas (RQ = 0.27 

for monocots; RQ = 0.9 for dicots). 

Listed Plant Species RQ=

The LOC was exceeded for listed monocots and dicots located in 

semi-aquatic areas exposed adjacent to treatment (RQ = 6.7 for 

monocots and 14.29 for dicots). For plants in adjacent dry areas, the 

LOC was exceeded only for dicots (RQ = 1.68). For spray drift

alone, RQ for dicots exceed the LOC (RQ = 2.24), but no RQs for 

monocots (RQ = 0.28) exceed the LOC. 

Additions: 

Results of the AgDRIFT model show that the distance of the risk to 

listed plants via spray drift can range from 7 feet to 492 feet off the 

edge of treated site. That is, beyond 492 ft from the treated field, 

the RQ values are below the LOC of 1.

5. SUMMARY – LISTED SPECIES (Direct and Indirect Effects)

Based on estimated maximum application rates, exposure levels and available effects data, 

amicarbazone used as an herbicide for control of certain annual grasses and annual broadleaf 

weeds and sedges on turf grass sites indicates direct effects LOC exceedances for birds, 

mammals, and terrestrial plants including alga. Therefore, there is a potential for indirect effects 

to listed animal and plant taxa that depend on those taxa directly at risk when exposed to 

amicarbazone as pollinators or seed dispersers, mammal or reptile burrows for habitat, feeding, 

or cover requirements, and for survival, growth, or reproduction. Listed species potentially at 

risk from direct or indirect effects from exposure to amicarbazone are presented in Table 6.

Table 6. Potential listed species risks associated with direct or indirect effects due to the use of 

amicarbazone on turf and conifers. 

Listed Taxon Direct Effects from

Acute Exposures

Direct Effects from

Chronic Exposures

Indirect Effects

Aquatic

Aquatic non-vascular plants Yes N/A Yes

Aquatic vascular plants No N/A Yes

Freshwater invertebrates No No Yes

Marine/estuarine invertebrates No No Yes

Freshwater fish No No Yes
10

Table 6. Potential listed species risks associated with direct or indirect effects due to the use of 

amicarbazone on turf and conifers. 

Listed Taxon Direct Effects from

Acute Exposures

Direct Effects from

Chronic Exposures

Indirect Effects

Marine/estuarine fish No No Yes

Aquatic-phase amphibians No No Yes

Terrestrial

Semi-aquatic plants - monocots Yes N/A Yes

Semi-aquatic plants - dicots Yes N/A Yes

Terrestrial plants – monocots No N/A Yes

Terrestrial plants - dicots Yes N/A Yes

Insects No N/A Yes

Birds No Yes Yes

Terrestrial-phase amphibians No Yes Yes

Reptiles No Yes Yes

Mammals No Yes Yes

N/A - indicates that this exposure route is not assessed.
11

APPENDIX I. AMICARBAZONE TOXICITY PROFILE

Appendix I. Amicarbazone Toxicity Profile for Aquatic Organisms and Plants

Species 

(scientific name) Test Material Endpoint Mean-Measured 

Conc. (mg a.i./L)

Study 

Classification

Reference

(MRID)

Freshwater Fish

Oncorhynchus mykiss

(Rainbow trout) TGAI LC50 >120.4A

Supplemental 45121807

Lepomis macrochirus

(Bluegill sunfish) TGAI LC50 >128.5 Supplemental 45121804

Pimephales promelas

(Fathead minnow)

TGAI NOAEC 7.3 Acceptable 45121602

Freshwater Invertebrates

Daphnia magna

(Water flea)

TGAI EC50 40.8 Acceptable 45121808

TGAI LOAEC 0.252 Supplemental 45121822

Estuarine/marine Fish

Cyprinodon variegatus

(Sheepshead minnow) TGAI

LC50 >118 Acceptable 45121806

NOAEC 2.3 mg a.i./L Acceptable 48237510

Estuarine/marine Invertebrates

Crassostrea gigas

(Pacific oyster) TGAI EC50 >63 Supplemental 45121809

Mysidopsis bahia

(Mysid shrimp)

TGAI LC50 109.7 Acceptable 45121810

TGAI NOAEC 5.0 Supplemental 45121601

Aquatic Plants

Lemna gibba

(Duckweed) TGAI

EC50 0.21

Acceptable 45121805

NOAEC 0.0406

Navicula pelliculosa

(Diatom) TGAI EC50 0.1 Acceptable 45121812

NOAEC 0.098

Anabaena flos-aquae

(Blue-green algae) TGAI

EC50 0.186

Acceptable 45121825

NOAEC 0.017

Skeletonema costatum

(Marine diatom) TGAI

EC50 0.017

Acceptable 45121818

NOAEC 0.0057

Selenastrum 

capricornutum

(Green algae)

TGAI

EC50 0.084

Acceptable 45121820

NOAEC 0.013

Deg of TGAI

(Desamino)

EC50 >1.35

Acceptable 45121827

NOAEC 1.35
12

Appendix I. Amicarbazone Toxicity Profile for Terrestrial Wildlife and Plants

Species

(common name) Test Material Endpoint Mean Concentration(Endpoint affected)

Study 

Classification

Reference 

(MRID)

WILDLIFE

Upland Game Birds and Waterfowls

Colinus virginianus

(Northern bobwhite 

quail)

TGAI

LD50 1965 mg a.i./kg bw Supplemental 45121811

LC50 >5690 mg a.i./kg diet Acceptable 48237511

NOAEC

27.1 mg a.i./kg diet

(reduction in no. of 

viable embryos and % 

hatchling per eggs set)

Acceptable 45121819

Deg of TGAI

(Desamino) NOAEC 1030 mg a.i./kg diet Acceptable 48237515

Deg of TGAI 

(N-Methyl 

Desamino)

NOAEC

413 mg a.i./kg diet

(hatchling and survivor 

body weights)

Acceptable 48237514

Anas platyrhynchos

(Mallard duck)

TGAI

LD50 See bobwhite quail data N/A N/A

LC50 >10536 mg a.i./kg diet Acceptable 45121815

NOAEC

27.1 mg a.i./kg diet

(decreased eggshell 

thickness)

Acceptable 45121814

Deg of TGAI

(Desamino) NOAEC

159 mg a.i./kg diet

(numerous endpoints 

affected) 

Acceptable 48237512

Deg of TGAI 

(N-Methyl 

Desamino)

NOAEC

<161 mg a.i./kg diet

(numerous endpoints 

affected)

Supplemental 48237513

Passerine Birds

No data TGAI LD50 No data No data No data

Mammals

Rattus norvegicus

(laboratory rat) TGAI

LD50 1087 mg a.i./kg bw Acceptable 45121605

NOAEC

Systemic = 6.4/7.3 

Reproductive = 

73.2/84.0

Offspring = 6.4/7.3 

mg/kg/day

[6.4 mg/kg/day 

equivalent to 100 

mg/kg diet]

Acceptable 45121625

Terrestrial Insects

Apis mellifera

(Honeybee) TGAI LD50 24.4 μg a.i./bee Supplemental 45121816

TERRESTRIAL PLANTS

Seedling Emergence
13

Monocot; Onion

TEP

EC25 0.1 lb a.i./A

Acceptable 45121821

EC05

1

0.034 lb a.i./A

Dicot; Sugar beet

(Beta vulgaris)

EC25 0.03 lb a.i./A

NOAEC 0.016 lb a.i./A

Monocot; Wheat Deg of TEP 

(N-Methyl 

Desamino)

EC25 >0.418 lb a.i./A

Acceptable 48237517

NOAEC 0.00493 lb a.i./A

Dicot; Sugar beet

(Beta vulgaris)

EC25 0.0866 lb a.i./A

EC05

1

0.00465 lb a.i./A

Monocot; Ryegrass 

(Lolium perenne) Deg of TEP 

(Desamino)

EC25 >0.38 lb a.i./A

Acceptable 48237516

NOAEC 0.0049 lb a.i./A

Dicot; Cabbage 

(Brassica oleracea)

EC25 0.16 lb a.i./A

NOAEC 0.061 lb a.i./A

Vegetative Vigor

Monocot: Onion

TEP

EC25 0.026 lb a.i./A

Acceptable 45121821

NOAEC 0.016 lb a.i./A

Dicot; Buckwheat

EC25 0.0037 lb a.i./A

NOAEC 0.002 lb a.i./A

Monocot; Corn Deg of TEP 

(N-Methyl 

Desamino)

EC25 >0.457 lb a.i./A

Acceptable 48237518

NOAEC 0.457 lb a.i./A

Dicot; Sugar beet

(Beta vulgaris)

EC25 0.0251 lb a.i./A

NOAEC 0.00532 lb a.i./A

Monocot; Wheat 

(Triticum astivum) Deg of TEP

(Desamino)

EC25 >0.30 lb a.i/A

Acceptable 48237520

NOAEC 0.019 lb a.i./A

Dicot; Sugar beet 

(Beta vulgaris)

EC25 0.318 lb a.i./A

EC05* 0.122 lb a.i./A

A Values in bold for each taxonomic groups are used in the risk assessment to assess acute and/or chronic risk to 

these groups

1 An EC05 is used when the NOAEC is below the lowest test concentration or above the EC25.

* NOAEC was <0.0057 lb a.i./A
14

APPENDIX II. AQUATIC RISK ASSESSMENT FOR TURF USES

A. Freshwater Fish and Invertebrates

Table II1. Tier II Risk Quotients for Freshwater Organisms for Proposed Amicarbazone Use 

on Turf 

1, 2

Use PRZM

Scenario

Peak 

EEC

21D 

EEC

60D 

EEC

Freshwater Fish

(LC50 >120.4 mg a.i./L ; 

NOAEC = 7.3 mg a.i./L) 

Freshwater Invertebrate

(EC50 = 40.8 mg a.i./L ; 

LOAEC = 0.252 mg a.i./L*)

μg a.i./L Acute4 Chronic5 Acute6 Chronic7

Turf and 

Conifers FL nursery3

8.97 7.48 6.21 <0.01 <0.1 <0.01 0.03

1 LOC exceedances are bolded (Endangered Species LOC > 0.05; Acute Restricted LOC > 0.1; Acute Risk > 0.5 and Chronic LOC 

>1). 

2 EECs from Section 2.

3 Highest exposure scenario selected for new non-crop uses (turf and conifers) based on a suite of PRZM/EXAMS scenarios 

modeled at 1 application at 0.044 lb a.i./A

* The LOAEC was used as a surrogate for the NOAEC. The NOAEC was <0.252 mg/L, a non-definitive endpoint.

4 RQ = peak EEC ÷ fish LC50; 5 RQ = 60-Day EEC ÷ fish NOAEC; 6 RQ = peak EEC ÷ invertebrate EC50; 7 RQ = 21-Day EEC ÷ 

invertebrate NOAEC

B. Estuarine/Marine Fish and Invertebrates

Table II2. Tier II Risk Quotients for Estuarine/marine Organisms for Proposed Amicarbazone

Use on Turf 

1, 2

Use PRZM

Scenario

Peak 

EEC

21D 

EEC

60D 

EEC

Marine/Estuarine Fish 

(LC50 >118 mg a.i./L

NOAEC = 2.3 mg 

a.i./L)

Marine/Estuarine 

Invertebrate

(EC50 = 109.7 mg a.i./L;

NOAEC = 5 mg a.i./L)

μg a.i./L Acute4 Chronic5 Acute6 Chronic7

Turf and 

Conifers FL nursery3

8.97 7.48 6.21 <0.01 <0.1 <0.01 <0.1

1 LOC exceedances are bolded (Endangered Species LOC > 0.05; Acute Restricted LOC > 0.1; Acute Risk > 0.5 and Chronic LOC >1). 

2 EECs from Section 2.

3 Highest exposure scenario selected for new non-crop uses (turf and conifers) based on a suite of PRZM/EXAMS scenarios modeled at 

1 application of 0.44 lb a.i./A 

4 RQ = peak EEC ÷ fish LC50; 5 RQ = 60-Day EEC ÷ fish NOAEC; 6 RQ = peak EEC ÷ invertebrate EC50; 7 RQ = 21-Day EEC ÷ 

invertebrate NOAEC
15

C. Aquatic Plants

Table II3. Tier II Risk Quotients for Proposed Amicarbazone Use on Turf 

1,2

Use PRZM 

Scenario

Peak EEC

(μg a.i./L) 

Vascular Aquatic Plant

(EC50 = 210 μg a.i./L

NOAEC = 40.6 μg a.i./L)

Non-Vascular Aquatic Plant

(EC50 = 17 μg a.i./L;

NOAEC = 5.7 μg a.i./L)

Non-target4 Listed5 Non-target6 Listed7

Turf and 

Conifers FL nursery3

8.97 0.04 0.2 0.5 1.6

1 LOC exceedances are bolded (Non-listed plant LOC > 1; Listed plant LOC > 1). 

2 EECs from Section 2.

3 Based on highest exposure scenario modeled for turf and conifers (1 app at 0.44 lb a.i./A)

4 RQ = peak EEC ÷ vascular EC50; 5 RQ = peak EEC ÷ vascular NOAEC; 6 RQ = peak EEC ÷ non-vascular EC50; 7RQ = peak EEC 

÷ non-vascular NOAEC
16

APPENDIX III. PRZM/EXAMS OUTPUT FOR TURF USE

stored as FLnursery31.out

Chemical: amicarbazone

PRZM environment: FLnurserySTD.txt modified Tueday, 26 August 2008 at 06:16:38

EXAMS environment: pond298.exv modified Tueday, 26 August 2008 at 06:14:08

Metfile: w12839.dvf modified Tueday, 26 August 2008 at 06:14:20

Water segment concentrations (ppb)

Year Peak 96 hr 21 Day60 Day90 DayYearly

1961 1.963 1.938 1.844 1.64 1.405 0.5188

1962 1.71 1.692 1.622 1.491 1.396 0.5745

1963 0.344 0.341 0.328 0.3156 0.306 0.1529

1964 0.2912 0.2871 0.2732 0.2533 0.2475 0.09762

1965 3.698 3.634 3.381 2.961 2.726 1.087

1966 0.5703 0.5626 0.5403 0.4792 0.4328 0.1585

1967 0.4355 0.4313 0.4141 0.3755 0.3337 0.1234

1968 0.2622 0.2596 0.2468 0.235 0.2109 0.06323

1969 0.5983 0.5932 0.5611 0.4992 0.4482 0.1659

1970 2.569 2.539 2.431 2.177 1.833 0.6754

1971 0.3862 0.3831 0.37 0.3412 0.3344 0.1621

1972 1.414 1.397 1.329 1.211 1.03 0.3462

1973 0.3733 0.3695 0.3507 0.313 0.2988 0.136

1974 2.924 2.888 2.718 2.382 2.162 0.8415

1975 0.4087 0.4051 0.3908 0.3588 0.3386 0.1831

1976 2.098 2.063 1.979 1.583 1.328 0.5076

1977 1.933 1.912 1.829 1.659 1.312 0.4042

1978 4.795 4.74 4.604 4.133 3.72 1.655

1979 4.787 4.227 3.297 2.649 2.305 1.018

1980 15.3 14.96 13.66 10.72 8.888 3.309

1981 0.6338 0.6291 0.6097 0.5819 0.5447 0.3006

1982 1.869 1.84 1.728 1.299 1.03 0.3424

1983 0.8761 0.8674 0.8344 0.7647 0.7004 0.2867

1984 11.91 11.53 10.47 8.44 6.853 2.293

1985 0.6998 0.6936 0.6664 0.6181 0.581 0.286

1986 9.432 9.084 7.799 6.441 5.527 1.999

1987 1.246 1.232 1.174 1.073 0.9882 0.4655

1988 0.4866 0.4779 0.4576 0.3863 0.3447 0.158

1989 0.2805 0.2779 0.2674 0.245 0.2284 0.09557

1990 0.3143 0.3073 0.2769 0.2538 0.2209 0.08319

Sorted results

Prob. Peak 96 hr 21 Day60 Day90 DayYearly

0.032258064516129 15.3 14.96 13.66 10.72 8.888 3.309

0.0645161290322581 11.91 11.53 10.47 8.44 6.853 2.293

0.0967741935483871 9.432 9.084 7.799 6.441 5.527 1.999
17

0.129032258064516 4.795 4.74 4.604 4.133 3.72 1.655

0.161290322580645 4.787 4.227 3.381 2.961 2.726 1.087

0.193548387096774 3.698 3.634 3.297 2.649 2.305 1.018

0.225806451612903 2.924 2.888 2.718 2.382 2.162 0.8415

0.258064516129032 2.569 2.539 2.431 2.177 1.833 0.6754

0.290322580645161 2.098 2.063 1.979 1.659 1.405 0.5745

0.32258064516129 1.963 1.938 1.844 1.64 1.396 0.5188

0.354838709677419 1.933 1.912 1.829 1.583 1.328 0.5076

0.387096774193548 1.869 1.84 1.728 1.491 1.312 0.4655

0.419354838709677 1.71 1.692 1.622 1.299 1.03 0.4042

0.451612903225806 1.414 1.397 1.329 1.211 1.03 0.3462

0.483870967741936 1.246 1.232 1.174 1.073 0.9882 0.3424

0.516129032258065 0.8761 0.8674 0.8344 0.7647 0.7004 0.3006

0.548387096774194 0.6998 0.6936 0.6664 0.6181 0.581 0.2867

0.580645161290323 0.6338 0.6291 0.6097 0.5819 0.5447 0.286

0.612903225806452 0.5983 0.5932 0.5611 0.4992 0.4482 0.1831

0.645161290322581 0.5703 0.5626 0.5403 0.4792 0.4328 0.1659

0.67741935483871 0.4866 0.4779 0.4576 0.3863 0.3447 0.1621

0.709677419354839 0.4355 0.4313 0.4141 0.3755 0.3386 0.1585

0.741935483870968 0.4087 0.4051 0.3908 0.3588 0.3344 0.158

0.774193548387097 0.3862 0.3831 0.37 0.3412 0.3337 0.1529

0.806451612903226 0.3733 0.3695 0.3507 0.3156 0.306 0.136

0.838709677419355 0.344 0.341 0.328 0.313 0.2988 0.1234

0.870967741935484 0.3143 0.3073 0.2769 0.2538 0.2475 0.09762

0.903225806451613 0.2912 0.2871 0.2732 0.2533 0.2284 0.09557

0.935483870967742 0.2805 0.2779 0.2674 0.245 0.2209 0.08319

0.967741935483871 0.2622 0.2596 0.2468 0.235 0.2109 0.06323

0.1 8.9683 8.6496 7.4795 6.2102 5.3463 1.9646

Average of yearly averages: 0.616330333333333

Inputs generated by pe5.pl - Novemeber 2006

Data used for this run:

Output File: FLnursery31

Metfile: w12839.dvf

PRZM scenario: FLnurserySTD.txt

EXAMS environment file: pond298.exv

Chemical Name: amicarbazone

Description Variable Name Value Units Comments

Molecular weight mwt 241 g/mol

Henry's Law Const. henry 1.5E-12 atm-m^3/mol

Vapor Pressure vapr 2.2E-8 torr

Solubility sol 4600 mg/L

Kd Kd mg/L

Koc Koc 16.7 mg/L
18

Photolysis half-life kdp 0 days Half-life

Aerobic Aquatic Metabolism kbacw 140 days Halfife

Anaerobic Aquatic Metabolism kbacs 0 days Halfife

Aerobic Soil Metabolism asm 69.9 days Halfife

Hydrolysis: pH 7 0 days Half-life

Method: CAM 1 integer See PRZM manual

Incorporation Depth: DEPI 0 cm

Application Rate: TAPP 0.49 kg/ha

Application Efficiency: APPEFF 0.99 fraction

Spray Drift DRFT 0.01 fraction of application rate applied to pond

Application Date Date 01-03 dd/mm or dd/mmm or dd-mm or dd-mmm

Record 17: FILTRA

IPSCND 1

UPTKF

Record 18: PLVKRT

PLDKRT

FEXTRC 0.5

Flag for Index Res. Run IR EPA Pond

Flag for runoff calc. RUNOFF none, monthly or total(average of entire run)
19

APPENDIX IV. T-REX v. 1.4.1 (Terrestrial Exposure Model for Birds and 

Mammals) Input and Output

Corn/Turf Use – Upper Bound (1 x 0.45 lb ai/A x 35D Half-life)

Upper Bound Kenaga Residues For RQ Calculation

Chemical Name: Amicarbazone

Use: Corn

Formulation:

Application Rate: 0.4484 lbs ai/A

Half-life: 35 days

Application Interval: N/A

Maximum # Apps./Year: 1 application

Length of Simulation: 1 year

Endpoints

Avian

Quail LD50 (mg/kg-bw) 1965

Quail LC50 (mg/kg-diet) >5690

NOAEL(mg/kg-bw) 0.00

Quail NOAEC (mg/kg-diet) 27.1

Mammals

Rat LD50 (mg/kg-bw) 1087

LC50 (mg/kg-diet) 0.00

Rat NOAEL (mg/kg-bw) 6.4

Rat NOAEC (mg/kg-diet) 100

Dietary-Based EECs 

Food Items Upper Bound EEC

(mg ai/kg)

Mean EEC

(mg ai/kg)

Short Grass

Tall Grass

Sm. Insects, Broadleaf Plants

Lg. Insects, Fruits, Pods

107.62

49.32

60.53

6.73

8.11

16.14

20.18

3.14

AVIAN EECs and ADJUSTED LD50s

Avian Class Body Weight 

(g)

Ingestion 

(Fdry)

(g bw/day)

Ingestion 

(Fwet)

(g/day)

% body wgt

consumed

FI

(kg-diet/day)

Small 20 5 23 114 2.28E-02

Mid 100 13 65 65 6.49E-02

Large 1000 58 291 29 2.91E-01
20

Avian Body 

Weight (g)

Adjusted LD50

(mg/kg-bw)

20 1415.64

100 1802.19

1000 2545.66

Dose-based EECs (mg/kg-bw)

Food items

Avian Classes and Body Weights

small mid large

20 g 100 g 1000 g

Upper Bound EEC (mg/kg)

Short Grass

Tall Grass

Sm. Insects, Broadleaf Plants

Lg. Insects, Fruits, Pods

122.56 69.89 31.29

56.18 32.03 14.34

68.94 39.31 17.60

7.66 4.37 1.96

Mean EEC (mg/kg)

Short Grass

Tall Grass

Sm. Insects, Broadleaf Plants

Lg. Insects, Fruits, Pods

43.41 24.75 11.08

18.38 10.48 4.69

22.98 13.10 5.87

3.57 2.04 0.91

MAMMALIAN EECs and ADJUSTED LD50s

Mammalian

Class

Body

Weight

Ingestion 

(Fdry)

(g bwt/day)

Ingestion 

(Fwet)

(g/day)

% body wgt

consumed

FI

(kg-diet/day)

Herbivores/

insectivores

15 3 14 95 1.43E-02

35 5 23 66 2.31E-02

1000 31 153 15 1.53E-01

Granivores

15 3 3 21 3.18E-03

35 5 5 15 5.13E-03

1000 31 34 3 3.40E-02

Mammalian

Class

Body 

Weight

Adjusted

LD50

Adjusted

NOAEL

Herbivores/

insectivores

15 2389.04 14.07

35 1932.99 11.38

1000 836.08 4.92

Granivores

15 2389.04 14.07

35 1932.99 11.38

1000 836.08 4.92
21

Dose-based EECs 

(mg/kg-bw)

Mammalian Classes and Body Weight

Herbivores and Insectivores Granivores

15 g 35 g 1000 g 15 g 35 g 1000 g

Upper Bound EECs (mg ai/kg)

Short Grass 102.60 70.91 16.44

Tall Grass 47.03 32.50 7.54

Broadleaf plants and small insects 57.71 39.89 9.25

Fruits/pods/seeds/large insects 6.41 4.43 1.03 1.43 0.98 0.23

Mean EECs (mg ai/kg)

Short Grass 36.34 25.11 5.82

Tall Grass 15.39 10.64 2.47

Broadleaf plants and small insects 19.24 13.30 3.08

Fruits/pods/seeds/large insects 2.99 2.07 0.48 0.67 0.46 0.11

RESULTS – Upper Bound EECs and RQs

Table IV1. Upper Bound Kenaga, Acute Avian Dose-Based Risk Quotients

Size Class

(grams)

Adjusted

LD50

EECs and RQs

Short Grass Tall Grass

Broadleaf 

Plants/

Small Insects

Fruits/Pods/

Seeds/

Large Insects

EEC RQ EEC RQ EEC RQ EEC RQ

20 1415.64 122.56 0.09 56.18 0.04 68.94 0.05 7.66 0.01

100 1802.19 69.89 0.04 32.03 0.02 39.31 0.02 4.37 0.00

1000 2545.66 31.29 0.01 14.34 0.01 17.60 0.01 1.96 0.00

Bold value indicates LOC exceedance

Table IV2. Upper Bound Kenaga, Subacute Avian Dietary Based Risk Quotients

LC50

EECs and RQs

Short Grass Tall Grass

Broadleaf 

Plants/

Small Insects

Fruits/Pods/

Seeds/

Large Insects

EEC RQ EEC RQ EEC RQ EEC RQ

>5690 107.62 <0.02 49.32 <0.01 60.53 <0.01 6.73 <0.01

Size class not used for dietary risk quotients 

Bold value indicates LOC exceedance

Table IV3. Upper Bound Kenaga, Chronic Avian Dietary Based Risk Quotients

NOAEC 

(mg/kg-diet)

EECs and RQs

Short Grass Tall Grass

Broadleaf 

Plants/

Small Insects

Fruits/Pods/

Seeds/

Large Insects

EEC RQ EEC RQ EEC RQ EEC RQ

27 107.62 3.97 49.32 1.82 60.53 2.23 6.73 0.25

Size class not used for dietary risk quotients

Bold values indicate LOC exceedances
22

Table IV4. Upper Bound Kenaga, Acute Mammalian Dose-Based Risk Quotients

Size Class

(grams)

Adjusted

LD50

EECs and RQs

Short Grass Tall Grass

Broadleaf 

Plants/

Small Insects

Fruits/Pods/

Seeds/

Large Insects

Granivores

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ

15 2389.04 102.60 0.04 47.03 0.02 57.71 0.02 6.41 0.00 1.43 0.00

35 1932.99 70.91 0.04 32.50 0.02 39.89 0.02 4.43 0.00 0.98 0.00

1000 836.08 16.44 0.02 7.54 0.01 9.25 0.01 1.03 0.00 0.23 0.00

Table IV5. Upper Bound Kenaga, Chronic Mammalian Dietary Based Risk Quotients

NOAEC 

(mg/kg-diet)

EECs and RQs

Short Grass Tall Grass Broadleaf Plants/

Small Insects

Fruits/Pods/

Seeds/

Large Insects

EEC RQ EEC RQ EEC RQ EEC RQ

100 107.62 1.08 49.32 0.49 60.53 0.61 6.73 0.07

Size class not used for dietary risk quotients 

Table IV6. Upper Bound Kenaga, Chronic Mammalian Dose-Based Risk Quotients

Size Class

(grams)

Adjusted 

NOAEL

EECs and RQs

Short Grass Tall Grass

Broadleaf 

Plants/

Small Insects

Fruits/Pods/

Seeds/

Large Insects

Granivores

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ

15 14.07 102.60 7.29 47.03 3.34 57.71 4.10 6.41 0.46 1.43 0.10

35 11.38 70.91 6.23 32.50 2.86 39.89 3.50 4.43 0.39 0.98 0.09

1000 4.92 16.44 3.34 7.54 1.53 9.25 1.88 1.03 0.21 0.23 0.05

Bold values indicate LOC exceedances
23

Appendix V. Terrestrial Plants Exposure Modeling and Results (TerrPlant v 

1.2.2)

1. Ground Application of 0.4484 lbs ai/A to Corn/Turf

Table V1. Chemical Identity.

Chemical Name Amicarbazone

PC code 114004

Use Corn/Turf

Application Method Ground

Application Form liquid 

Solubility in Water (ppm) 4,600

Table V2. Input parameters used to derive EECs.

Input Parameter Symbol Value Units

Application Rate A 0.4484 lbs ai/A

Incorporation I 1 none

Runoff Fraction R 0.05 none

Drift Fraction D 0.01 none

Table V3. EECs for Flutriafol 125 g/l SC. Units in lbs ai/A.

Description Equation EEC

Runoff to dry areas (A/I)*R 0.02242

Runoff to semi-aquatic areas (A/I)*R*10 0.2242

Spray drift A*D 0.004484

Total for dry areas ((A/I)*R)+(A*D) 0.026904

Total for semi-aquatic areas ((A/I)*R*10)+(A*D) 0.228684

Table V4. Plant survival and growth data used for RQ derivation. Units are in lbs ai/A.

Seedling Emergence Vegetative Vigor

Plant type EC25 NOAEC EC25 NOAEC 

Monocot 0.1 0.034 0.026 0.016

Dicot 0.03 0.016 0.0037 0.002

Table V5. RQ values for plants in dry and semi-aquatic areas exposed to Flutriafol 125 g/l SC through 

runoff and/or spray drift.*

Plant Type Listed Status Dry Semi-Aquatic Spray Drift

Monocot non-listed 0.27 2.29 0.17

Monocot listed 0.79 6.73 0.28

Dicot non-listed 0.90 7.62 1.21

Dicot listed 1.68 14.29 2.24

*If RQ > 1.0, the LOC is exceeded (bolded), resulting in potential for risk to that plant group.

INC - inconclusive
24

APPENDIX VI. DATA EVALUATION RECORDS AND STUDY CLASSIFICATION OF 

NEWLY SUBMITTED DATA

OPPTS 

Guideline 

No.

Test 

Material MRID Study Classification Comments

850.1400 TGAI -Parent 48237510 Acceptable None

850.2200 TGAI- Parent 48237511 Acceptable None

850.2300 Deg of TGAIDesamino48237512 Acceptable None

850.2300 Deg of TGAIN-methyl48237513 Supplemental NOAEC is below lowest 

dose. A new study with 

lower doses is needed. 

850.2300 Deg of TGAIN-methyl48237514 Acceptable None

850.2300 Deg of TGAIDesamino48237515 Acceptable None

850.4100 Deg of TEPDesamino48237516 Acceptable None

850.4100 Deg of TEPN-methyl48237517 Acceptable None

850.4150 Deg of TEPN-methyl48237518 Acceptable None

850.4150 TEP- Parent 48237519 Acceptable Tomato and sugar beet were 

repeated to satisfy MRID 

45121821

850.4150 Deg of TEP -

Desamino

48237520 Acceptable None
25