Berol R648NG Tox Summary 

Assessment of the toxicity (10 day LC50) of 

Berol R648 NG to the Marine crustacean 

Corophium volutator

Berol R648 NG exhibited a 10 day LC50 value of 

1027mg.kg-1 (via treated seawater) to the marine 

amphipod Corophium volutator in the sediment 

phase. 

Acute Toxicity of Quaternary C12-14 alkyl 

methylamine chloride [BEROL R648 NG] to 

water fleas, daphnia magna 

The NOEC was determined as 1.2 mg/L and the 

LOEC was determined as 2.7mg/L. (Nominal 

Concentrations) 

The EC50 (48h) was calculated as 2.58 mg/L. 

(Nominal Concentrations) 

Toxicology information presented below is based on information from Structural Analog 

Relationship (SAR) chemical substances (see SAR comparisons) 

Study Name Result 

Determination of the primary and ultimate 

biodegradation of quaternary ammonium 

compounds, coco alkyl (hydroxyethyl), 

dimethyl, ethoxylated, chlorides in a 

Continuously-Fed activated sludge unit treating 

domestic waste water 

The quaternary ammonium compounds, coco 

alkyl (hydroxyethyl) dimethyl ethoxylated 

chlorides, are removed from the wastewater in 

conventional biological wastewater treatment

plants. Quaternary ammonium compounds, coco 

alkyl (hydroxyethyl) dimethyl ethoxylated 

chlorides are primarily removed by 

biodegradation. 

Chronic Toxicity OF BEROL R-648 (CAS 

863679-20-3) to Daphnia Magna in a 21 day 

reproduction test under semi-static conditions 

9 day NOEC Reproduction 0.36 mg/L 

9 day LC 100 (Adult Mortality) 1.5 mg/L

Acute toxicity of ETHOQUAD MC 1214/25 to 

Danio Rerio 

LC50/96h 29 mg/l 

Acute toxicity of ETHOQUAD MC 1214/25 to 

Water Fleas, Daphnia Magna 

EC50/48h 1 mg/l 

Effects of ETHOQUAD MC 1214/25 on the 

growth of the freshwater green algae 

pseudokirchneriella subcapitata 

EC50/72h 1.56 mg/l 

Biodegradability of Ethoquad 1214M/25 in the 

Closed Bottle Test 

63% at day 28. Readily biodegradable 

Acute Oral Toxicity Study in Rats 

(ETHOQUAD IMC/25) 

 LD50 300-2000mg/kg 

Local Lymph Node Assay (LLNA) in Mice 

(Identification of Contact Allergens) (Ethoquad 

IMC/25)

Sensitation potential is indicated in this study. To 

differentiate local irritation effect from possible 

sensitation, a Modified Local Lymph Node Assay 

will be performed. 

Modified Murine Local Lymph Node Assay 

(LLNA) Challenge Experiment) (Ethoquad 

1MCD/25) 

In a modified Local Lymph Node Assay, lymph 

node proliferation induced by severe irritation, 

was differentiated from possible sensitation. 

Primary Eye Irritation Study in Rabbits 

(Ethoquad IMC/25)

Irritatiing, lesions in cornea and iris persisting at 

observation day 21. Mean score corneal opacity 

1,00, iris 0,67, Reddening of cunjuncticae 2,00 

chemosis, 2,00. 

Berol R648 NG 

Berol R648 NG 

Berol R648NG 

[BEROL R648 NG] 

of quaternary ammonium

compounds, coco alkyl (hydroxyethyl),

dimethyl, ethoxylated, chlorides 

The quaternary ammonium compounds, coco 

alkyl (hydroxyethyl) dimethyl ethoxylated

chlorides, a

Quaternary ammonium compounds, coco

alkyl (hydroxyethyl) dimethyl ethoxylated

chlorides

BEROL R-648 (CAS

863679-20-3) t

f ETHOQUAD MC 1214/25 

f ETHOQUAD MC 1214/2

f ETHOQUAD MC 1214/25 

f Ethoquad 1214M/25 

(ETHOQUAD IMC/25)

IMC/25)

) (Ethoquad 

(Ethoquad

1MCD/25)

(Ethoquad IMC/25)