### Berol R648NG Tox Summary Assessment of the toxicity (10 day LC50) of Berol R648 NG to the Marine crustacean Corophium volutator Berol R648 NG exhibited a 10 day LC50 value of 1027mg.kg

- 1 (via treated seawater) to the marine amphipod Corophium volutator in the sediment phase. Acute Toxicity of Quaternary C12-14 alkyl methylamine chloride [BEROL R648 NG] to water fleas, daphnia magna The NOEC was determined as 1.2 mg/L and the LOEC was determined as 2.7mg/L. (Nominal Concentrations) The EC50 (48h) was calculated as 2.58 mg/L. (Nominal Concentrations)


### Toxicology information presented below is based on information from Structural Analog Relationship (SAR) chemical substances (see SAR comparisons) Study Name Result Determination of the primary and ultimate biodegradation of quaternary ammonium compounds, coco alkyl (hydroxyethyl), dimethyl, ethoxylated, chlorides in a Continuously

- Fed activated sludge unit treating domestic waste water The quaternary ammonium compounds, coco alkyl (hydroxyethyl) dimethyl ethoxylated chlorides, are removed from the wastewater in conventional biological wastewater treatment plants. Quaternary ammonium compounds, coco alkyl (hydroxyethyl) dimethyl ethoxylated chlorides are primarily removed by biodegradation. Chronic Toxicity OF BEROL R-648 (CAS 863679 -20-3) to Daphnia Magna in a 21 day reproduction test under semi-static conditions 9 day NOEC Reproduction 0.36 mg/L 9 day LC 100 (Adult Mortality) 1.5 mg/L Acute toxicity of ETHOQUAD MC 1214/25 to Danio Rerio LC50/96h 29 mg/l Acute toxicity of ETHOQUAD MC 1214/25 to Water Fleas, Daphnia Magna EC50/48h 1 mg/l Effects of ETHOQUAD MC 1214/25 on the growth of the freshwater green algae pseudokirchneriella subcapitata EC50/72h 1.56 mg/l
Biodegradability of Ethoquad 1214M/25 in the Closed Bottle Test 63% at day 28. Read ily biodegradable Acute Oral Toxicity Study in Rats (ETHOQUAD IMC/25) LD50 300-2000mg/kg Local Lymph Node Assay (LLNA) in Mice (Identification of Contact Allergens) (Ethoquad IMC/25) Sensitation potential is indicated in this study. To differentiate local irritation effect from possible sensitation, a Modified Local Lymph Node Assay will be performed. Modified Murine Local Lymph Node Assay (LLNA) Challenge Experiment) (Ethoquad 1MCD/25) In a modified Local Lymph Node Assay, lymph node proliferation induced by severe irritation, was differentiated from possible sensitation. Primary Eye Irritation Study in Rabbits (Ethoquad IMC/25) Irritatiing, lesions in cornea and iris persisting at observation day 21. Mean score corneal opacity 1,00, iris 0,67, Reddening of cunjuncticae 2,00 chemosis, 2,00. Berol R648 NG Berol R648 NG 

### Berol R648NG

[BEROL R648 NG]

of q ua t ernar y a mm o n ium compounds, coco alkyl (hydroxyethyl), di met hyl , ethoxylated, chlorides The quaternary ammonium compounds, coco alkyl (hydroxyethyl) dimethyl ethoxylated chlorides, a Quaternary ammonium compounds, coco alkyl (hydroxyethyl) dimethyl ethoxylated chlorides
BEROL R

- 648 ( CAS 8636 79

- 2 0

- 3 ) t f ETHOQUAD MC 1214/25 f ETHOQUAD MC 1214/2 f ETHOQUAD MC 1214/25
f Ethoquad 1214M/25
( ETHOQUAD IMC / 25 )
IMC/25) ) (Ethoquad

(Ethoquad 1 MCD / 25 ) ( Et h o q ua d IMC / 25 )