# Patent

## Title
Agents useful for reducing amyloid precursor protein and treating demantia and methods of use thereof

## Abstract

The present invention provides compounds and methods of administering compounds to a subject that can reduce βAPP production and that is not toxic in a wide range of dosages. The present invention also provides non-carbamate compounds and methods of administering such compounds to a subject that can reduce βAPP production and that is not tocix in a wide range of dosages. It has been discovered that either the racemic or enantiomerically pure non-carbamate compounds can be used to decrease βAPP production.

## Background
CROSS REFERENCE TO RELATED APPLICATION
The present application claims priority to U.S. provisional application Ser. No. 60/245,329, filed Nov. 2, 2000, which application is incorporated herein fully by this reference.


BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to agents useful for reducing amyloid precursor protein and methods of use thereof.
2. Background Art
The major pathological hallmarks of Alzheimer's disease (AD), a progressive neurodegenerative condition leading to loss of memory, are characterized by the appearance of senile plaques which are primarily composed of Aβ and neurofibrillary tangle aggregates (Selkoe, 1997; Roberson and Harrell, 1997). Aβ, a 40–42 residue peptide, is derived from a larger protein, βAPP (695–770 amino acids), whose biological functions remain to be fully determined but whose pathological role may be separated on the basis of its final proteolysed form (Checler, 1995; Selkoe, 1997). βAPP derivatives are generated by three enzymatic activities termed α-, β- and γ-secretases, to produce different protein fragments that are either neuroprotective or amyloidogenic. An aspartyl protease with β-secretase like properties has been identified (Hussaain et al, 1999; Sinha et al., 1999; Vassar et al., 1999; Yan et al., 1999), that may serve as a therapeutic marker. However, its value as a target for drug development is complicated by its location within two membranes (plasma and Golgi apparatus). Furthermore, the role of alternative compensatory activities remains unclear. Indeed, a second enzyme, Thimet oligopeptidase, was found capable of β-secretase activity in transfected COS cells (Koike et al., 1999). A major pharmaceutical industry focus has been to look for agents that reduce amyloidogenic processing using compounds that can manipulate βAPP to produce non-amyloidogenic by-products. However, it is important to note that the role of alternative βAPP fragments in AD is unclear.
Regarding regulatory mechanisms involved in βAPP processing, environmental agents have been demonstrated to accelerate βAPP turnover into its pathological Aβ form (Selkoe, 1997). Furthermore, the cellular surrounding of neurons, particularly astrocytes and microglia, are additional and non-neuronal sources of βAPP (Funato et al., 1998; Akiyama et al., 2000). Thus, amyloid plaque occurrence is often associated with enlarged microglia which produce interleuken-1 (IL-1), a potent mediator of astroglial proliferation and βAPP production (Akiyama et al., 2000). The fact that IL-1 can influence this process suggests that signaling pathways induced by cytokines are interconnected with βAPP metabolism Another example of receptor-signaling association and βAPP homeostasis is demonstrated through the activation of muscarinic m1 and m3 receptors which modify βAPP synthesis and processing through MAP kinase dependent and independent pathways (Felder et al., 1993; Nitsch et al, 1992 and 1994). Reductions in muscarinic receptors, as in AD, may alter βAPP metabolism and result in subsequent Aβ deposition. Cholinergic system impairment has been reversed with moderate success by the use of anticholinesterases (Greig et al., 1995; Brossi et al., 1996), the only approved drugs for AD treatment.
A family of novel anticholinesterases, phenserine and analogues, has been synthesized. Phenserine dramatically improves cognitive performance in rodents and is in clinical trails (Greig et al., 1995; Patel et al., 1998). Studies of rats with forebrain cholinergic lesions that are known to dramatically increase βAPP in cholinergic projection areas have shown that phenserine can protect against this and additionally, reduce βAPP production in naive animals (Haroutunian et al., 1997). As both βAPP processing and cholinesterase activity are affected in the AD brain (Bronfman et al., 1996) and as the anticholinesterase, tacrine, has been shown to decrease βAPP and Aβ in neuronal cells in vitro (Lahiri et al., 1998), current studies have focused on the molecular changes induced by phenserine. In these studies, naturally-occurring phenserine (the (−)-enantiomer) was used.
It is the cholinergic action of anticholinesterases such as (−)-phenserine, rivastigmine (Exellon®, Novartis®), donepezil (Aricept®, Pfizer®), galanthamine (Jansen®), tacine (Cognex®, Warner Lambert®), (−)-physostigmine (Synapton®, Forest®), that provides the compounds their ability to improve cognitive performance in both animal models and humans. Likewise, it is the cholinergic action that is also dose limiting for these same compounds (nausea, sweating, GI effects) (Becker et al., 1991). Conversely, the (+)-enantiomers are unable to inhibit either acetylcholinesterase (AChE., EC 3.1.1.7.) or butyrylcholinesterase (BChE., EC 3.1.1.8.), and hence have no cholinergic action. The (+)-enantiomers are also unnatural isomers and thus, need to be synthesized. Synthetic procedures provide a mixture of (+)- and (−)-forms that require early separation into optically pure forms to eventually obtain the final products.
Additional advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The advantages of the invention will be realized and attained by means of the elements and combinations particularly pointed out in the appended claims. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention, as claimed.
SUMMARY OF THE INVENTION
The present invention provides compounds and methods of administering compounds to a subject that can reduce βAPP production and that is not toxic in a wide range of dosages. The present invention also provides non-carbamate compounds and methods of administering such compounds to a subject that can reduce βAPP production and that is not toxic in a wide range of dosages. It has been discovered that either the racemic or enantiomerically pure non-carbamate compounds can be used to decrease βAPP production.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 shows the synthesis of analogues of (+)-physostigmine.
FIG. 2 shows the transformation of (+)-oxindole to analogues of (+)-physostigmine. Compound numbers refer to compounds in Table 1.
FIG. 3 shows effects of (+) and (−)-phenserine treatment of SH-SY5Y neuroblastoma cells on βAPP protein levels and ERK transcription factor levels.
FIG. 4 shows effects of (−)-phenserine treatment of SK-N-SH neuroblastoma cells on extracellular βAPP protein levels (4A), intracellular βAPP protein levels (4B), toxicity (4C) and Aβ levels (4D).
FIG. 5 shows Aβ and βAPP levels in SK-N-SH cells after administration of (+)-phenserine.
FIG. 6 shows (−)-phenserine treatment of U373 MG astrocytoma alone or in combination with ERK and PI 3 kinase inhibitors.
FIG. 7 shows βAPP protein levels in SK-N-SH cells after administration of cymserine and its analogs.
FIG. 8 shows effects of (−)-phenserine treatment on reporter gene expression in the presence and absence of the βAPP-mRNA 5′ UTR (8A), intracellular βAPP protein levels (8B), and on βAPP RNA levels (8C) in transfected U373 MG astrocytoma cells.
FIG. 9 shows β-APP levels in transgenic mice after administration of (−)-phenserine and (−)-phenethylcymserine.
FIGS. 10A–10B show Aβ1-40 and Aβ1-42 levels in transgenic mice after administration of (−)-phenserine and (−)-phenethylcymserine derived from animals described in FIG. 9.
FIG. 11A shows the translational regulation of phenserine (Rate of APP Synthesis) in SH-SY5Y cells. FIG. 11B shows the effects of (−)-phenserine on steady state APP mRNA levels in SH-SY5Y cells.
FIGS. 12A and B show the effects of (−)-phenserine on extracellular and intracellular APP levels in SK-N-SH, respectively.
FIG. 13A shows the effects of (−)-phenserine on secreted APP levels and cell-viability in SH-SY5Y cells. FIGS. 13B–M show the effects of several compounds of the invention on secreted APP levels and cell-viabilty in SH-SY5Y cells.
FIG. 14 shows the effects of several compounds of the invention on extra- and intracellular APP levels in SH-SY5Y cells.
FIG. 15A shows the translational regulation by compounds (rate of APP Synthesis) in SH-SY5Y cells using several compounds of the invention. FIG. 15B shows the effects of several compounds of the invention on the steady state APP mRNA levels in SH-SY5Y cells.



DETAILED DESCRIPTION OF THE INVENTION
The present invention may be understood more readily by reference to the following detailed description of desired embodiments of the invention and the Examples included therein.
Before the present compounds, compositions and methods are disclosed and described, it is to be understood that this invention is not limited to specific synthetic methods, as such may, of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting. It must be noted that, as used in the specification and the appended claims, the singular forms “a,” “an” and “the” include plural referents unless the context clearly dictates otherwise.
Throughout this application, where publications and patents are referenced, the disclosures of these publications and patents in their entireties are hereby incorporated by reference into this application in order to more fully describe the state of the art to which this invention pertains.
Variables, such as R1–R15, n, A, D, E, G, X, Y, and Z throughout the application are the same variables as previously defined unless stated to the contrary.
In this specification and in the claims which follow, reference will be made to a number of terms which shall be defined to have the following meaning.
The term “alkyl” as used herein refers to a branched or unbranched saturated hydrocarbon group of 1 to 4, 1 to 8, or 1 to 20 carbon atoms, such as methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, t-butyl, pentyl, hexyl, heptyl, octyl, and the like. Examples of cycloalkyl groups include cyclopentyl and cyclohexyl.
The term “alkenyl” as used herein refers to a hydrocarbon group of 2 to 4, 2 to 8, or 2 to 20 carbon atoms and structural formula containing a carbon-carbon double bond.
The term “alkynyl” as used herein refers to a hydrocarbon group of 2 to 4, 2 to 8, or 2 to 20 carbon atoms and a structural formula containing a carbon-carbon triple bond.
The term “aryl” is defined as any carbon-based aromatic group including, but not limited to, phenyl, benzene, naphthalene, anthracene, phenanthrene, pyrene, and benzo[a]pyrene, etc.
The term “substituted aryl” is defined as an aryl group having at least one group attached to the aryl group that is not hydrogen. Examples of groups that can be attached to the aryl group include, but are not limited to, alkyl, alkynyl, alkenyl, aryl, heterocyclic, halide, nitro, amino, ester, ketone, aldehyde, hydroxy, carboxylic acid, alkoxy, cyano, alkoxy, thioalkyl, haloalkyl, hydroxyalkyl, alkylamino, diakylamino, or acyl. In various embodiments, a substituent is bound to carbon 2, 3, 4, 5, or 6 of one of these moieties. Examples of alkoxy substituents include, but are not limited to, methoxy, ethoxy, and isopropoxy groups. Examples of acyl substituents include acetyl and benzoyl groups.
The term “aralkyl” is defined as an aryl group having an alkyl, alkynyl, or alkenyl group attached to the aryl group. An example of an aralkyl group is a benzyl group.
The term “heteroaryl” is defined as an aryl group that has at least one heteroatom such as nitrogen, sulfur, or oxygen incorporated within the ring of the aryl group.
The term “heteroalkyl” is defined as an alkyl group that has at least one heteroatom, such as nitrogen, sulfur, oxygen, or phosphate, incorporated within the alkyl group or attached to the alkyl group.
The invention, in one aspect, relates to a compound having the formula I or II




    
    
        wherein R1 and R2 are, independently, hydrogen, branched or straight chain C1–C8 alkyl, substituted or unsubstituted aryl, or aralkyl;
        R3 is branched or straight chain C1–C4 alkyl or heteroalkyl or C4–C8 alkyl or heteroakyl, or substituted or unsubstituted aryl;
        X and Y are, independently, O, S, alkyl, hydrocarbon moiety, C(H)R4, or NR5, wherein R4 and R5 are, independently, hydrogen, oxygen, branched or straight chain C1–C8 alkyl, C2–C8 alkenyl, or C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl; and
        R6 is hydrogen; C1–C8 alkyl, C2–C8 alkenyl, C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl, or (CH2)nR7, where R7 is hydroxy, alkoxy, cyano, ester, carboxylic acid, substituted or unsubstituted amino, and n is from 1 to 4,
        wherein the compound having the formula I or II is the substantially pure (+)-enantiomer,
        with the proviso that the compound is not (+)-physostigmine, (+)-octylcarbamoyleseroline, (+)-benzylcarbamoyleseroline, (+)-physovenine, (+)-N-methylphysostigmine, (+)-phenserine, (+)-3-(1-methylamino)-ethyl-2H-indol-5yl-phenylcarbamate, (+)-N1-benzylnorphysostigmine, (+)-N1-benzylnorphenserine, (+)-N1-benzylnortolserine, (+)-N1-benzylnorcymserine, (+)-N1-norphysostigmine, (+)-N1-norphenserine, (+)-N1-nortolserine, (+)-N1-norcymserine, (+)-N8-benzylnorphysostigmine, (+)-N8-benzylnorphenserine, (+)-N8-norphysostigmine, (+)-N8-norphenserine, (+)-N1,N8-bisbenzylnorphysostigmine, (+)-N1,N8-bisbenzylnorphenserine, (+)-N1,N8-bisnorphysostigmine, or (+)-N1,N8-bisnorphenserine,
        with the proviso that when the compound is formula I, X is NCH3, and Y is NR5, where R5 is hydrogen, loweralkyl, arylloweralkyl, heteroarylloweralkyl, cycloalkylmethyl, or loweralkenyl methyl, R3 is not methyl or at least one of R1 or R2 is not H or alkyl.
    
    


The invention also relates to a compound having the formula III or IV




    
    
        wherein R1 and R2 are, independently, hydrogen, branched or straight chain C1–C8 alkyl, substituted or unsubstituted aryl or aralkyl;
        R3 is branched or straight chain C1–C4 alkyl, or substituted or unsubstituted aryl;
        X and Y are, independently, O, S, alkyl, hydrocarbon moiety, C(H)R4, or NR5, wherein R4 and R5 are, independently, hydrogen, oxygen, branched or straight chain C1–C8 alkyl, C2–C8 alkenyl or C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl; and
        R6 is hydrogen; C1–C8 alkyl, C2–C8 alkenyl, C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl, or (CH2)nR7, where R7 is hydroxy, alkoxy, cyano, ester, carboxylic acid, substituted or unsubstituted amino, and n is from 1 to 4,
    
    


The chiral center of compounds I–IV is the carbon atom that has R3 bonded to it. Here, the (+)-enantiomer has R3 pointing behind the plane of the page. In various embodiments, the compounds having the structure I or II have an enantiomeric purity for the (+)-enantiomer of from 55 to 100%, desirably from 75 to 100%, more desirably from 85 to 100%, more desirably from 95 to 100%, and even more desirably 100%.
In one embodiment, when the compound is formula I, R3 is methyl and X is NCH3.
In one embodiment, when the compound is formula I or II, R3 is not methyl. In particular embodiments, R3 is a branched or straight chain alkyl or heteroalkyl group of 2, 3, 4, 5, 6, 7, or 8 carbons or substituted or unsubstituted aryl.
In another embodiment, when the compound has the structure I or II, Y is C(H)R4 or X is O, S, or C(H)R4.
In another embodiment, when the compound is formula I, R3 is methyl, X is NCH3, and Y is NCH3. In one embodiment, when the compound is formula I, R3 is methyl, X is NCH3, Y is NCH3, and R1 is C1–C8 straight chain alkyl or benzyl and R2 is hydrogen. In one embodiment, when the compound is formula I, R3 is methyl, X is NCH3, Y is NCH3, and R1 is substituted or unsubstituted phenyl and R2 is hydrogen. In one embodiment, when the compound is formula I, R3 is methyl, X is NCH3, Y is NCH3, and R1 and R2 are, independently, methyl or ethyl.
In another embodiment, when the compound is formula I, R3 is methyl, X is NCH3, and Y is O. In one embodiment, when the compound is formula I, R3 is methyl, X is NCH3, Y is O, R1 is C1–C8 straight chain alkyl or benzyl, and R2 is hydrogen. In one embodiment, when the compound is formula I, R3 is methyl, X is NCH3, Y is O, and R1 and R2 are, independently, methyl or ethyl. In one embodiment, when the compound is formula I, R3 is methyl, X is NCH3, Y is O, and R1 is substituted or unsubstituted phenyl and R2 is hydrogen.
In another embodiment, when the compound is formula I, R3 is methyl, X is NCH3, and Y is S. In one embodiment, when the compound is formula I, R3 is methyl, X is NCH3, Y is S, R1 is C1–C8 straight chain alkyl or benzyl, and R2 is hydrogen. In one embodiment, when the compound is formula I, R3 is methyl, X is NCH3, Y is S, and R1 and R2 are, independently, methyl or ethyl. In one embodiment, when the compound is formula I, R3 is methyl, X is NCH3, Y is S, R1 is substituted or unsubstituted phenyl, and R2 is hydrogen.
In another embodiment, when the compound is formula I, R3 is methyl, X is NCH3, and Y is NR5. In one embodiment, when the compound is formula I, R3 is methyl, X is NCH3, and Y is NR5, wherein R5 is —CH2CH═CH2, —CH2CH2Ph, benzyl, or hydrogen.
In another embodiment, when the compound has the formula I, R3 is methyl, Y is NCH3, and X is NCH3, wherein R4 is benzyl or hydrogen.
In another embodiment, when the compound is formula I, R3 is methyl, X is NCH3, Y is NR5, wherein each R4 and R5 is, independently, hydrogen or benzyl.
In another embodiment, when the compound is formula I, R3 is phenyl, X is NCH3, and Y is NCH3.
In another embodiment, when the compound is formula I, R3 is methyl, and X is NCH3, and Y is not NH or NHCH2Ph.
In another embodiment, when the compound is formula II, R3 is methyl, X is C(H)CH3, and R6 is (CH2)2R7, where R7 is a substituted or unsubstituted amino group.
In another embodiment, the compound having the formula I or II can be found in Table 1. Although only the (+)-isomer is illustrated to save space, it is the intent of the invention to claim the (+)-isomer, (−)-isomer, and mixtures of both isomers (e.g., racemic 1:1 mixtures) of all of the compounds of the invention unless such compounds are specifically excluded.










TABLE 1





Structure
No.
R
Compounds











 1 2 3 4 5 6 7 8 910
CH3C2H5(CH2)2CH2(CH3)2(CH2)3CH3(CH2)4CH3(CH2)5CH3(CH2)6CH3(CH2)7CH3CH2C6H5
(+)-Physostigmine(+)-Ethylcarbamoyleseraline(+)-Propylcarbamoyleseroline(+)-Isopropylcarbamoylescroline(+)-Butylcarbamoyleseroline(+)-Pentylcarbamoyleseroline(+)-Hexylcarbamoyleseroline(+)-Heptylcarbamoyleseroline(+)-Octylcarbamayleseroline(+)-Benzylcarbamoyleseroline


 






11121314151617181920
CH3C2H5(CH2)2CH2(CH3)2(CH2)3CH3(CH2)4CH3(CH2)5CH3(CH2)6CH3(CH2)7CH3CH2C6H5
(+)-Physovenine(+)-Ethylcarbamoylphysovenol(+)-Propylcarbamoyl physovenol(+)-Isopropylcarbamoyl physovenol(+)-Butylcarbamoyl physovenol(+)-Pentylcarbamoyl physovenol(+)-Hexylcarbamoyl physovenol(+)-Heptylcarbamoylphysovenol(+)-Octylcarbamoyl physovenol(+)-Benzylcarbamoyl physovenol


 






21222324252627282930
CH3C2H5(CH2)2CH2(CH3)2(CH2)3CH3(CH2)4CH3(CH2)5CH3(CH2)6CH3(CH2)7CH3CH2C6H5
(+)-Thiaphysovenine(+)-Ethylcarbamoylthiaphysovenol(+)-Propylcarbamoylthia physovenol(+)-Isopropylcarbamoylthia physovenol(+)-Butylcarbamoylthia physovenol(+)-Pentylcarbamoylthia physovenol(+)-Hexylcarbamoylthia physovenol(+)-Heptylcarbamoylthiaphysovenol(+)-Octylcarbamoylthia physovenol(+)-Benzylcarbamoylthia physovenol


 






3132
CH3C2H5
(+)-N-Methylphysostigmine(+)-Diethylcarbamoyleseroline


 






3334
CH3C2H5
(+)-N-Methylphysovenine(+)-Diethylcarbamoylphysovenol


 






3536
CH3C2H5
(+)-N-Methylthiaphysovenine(+)-Diethylcarbamoylthiaphysovenol


 






373839404142434445464748495051525354
H2′-CH33′-CH34′-CH32′-CH2CH33′-CH2CH34′-CH2CH32′-CH2(CH3)23′-CH2(CH3)24′-CH2(CH3)22′,3′-CH32′,4′-CH32′,5′-CH32′,6′-CH33+,4′-CH33′,5′-CH33′,6′-CH32′,4′,6′-CH3
(+)-Phenserine(+)-Tolserine(+)-3′-Methylphenserine(+)-4′-Methylphenserine(+)-2′-Ethylphenserine(+)-3′-Eethylphenserine(+)-4-Eethylphenserine(+)-2′-Isopropylphenserine(+)-3′-Isopropylphenserine(+)-Cymserine(+)-2′,3′Dimethylphenserine(+)-2′,4′-Dimethylphenserine(+)-2′,5′-Dimethylphenserine(+)-2′,6′-Dimetheylphenserine(+)-3′,4′-Dimethylphenserine(+)-3′,5′-Dimethylphenserine(+)-3′,6-Dimethylphenserine(+)-2′,4′,6′-Trimethylphenserin


 






555657585960616263646566676869707172
H2′-CH33′-CH34′-CH32′-CH2CH33′-CH2CH34′-CH2CH32′-CH2(CH3)23′-CH2(CH3)24′-CH2(CH3)22′,3′-CH32′,4′-CH32′,5′-CH32′,6′-CH33+,4′-CH33′,5′-CH33′,6′-CH32′,4′,6′-CH3
(+)-Phenylcarbamoyl-physovenol(+)-2′-Methylphenylcarbamoyl-physovenol(+)-3′-Methylphenylcarbamoyl-physovenal(+)-4′-Methyl phenylcarbamoyl-physovenol(+)-2′-Ethylphenylcarbamoyl-physovenal(+)-3′-Eethylphenylcarbamoyl-physovenol(+)-4′-Eethylphenylcarbamoyl-physovenol(+)-2′-Isopropylphenylcarbamoyl-physovenol(+)-3′-Isopropylphenylcarbamoyl-physovenol(+)-4′-Isopropylphenylcarbamoyl-physovenol(+)-2′,3′-Dimethylphenylcarbamoyl-physovenol(+)-2′,4′-Dimethylphenylcarbamoyl-physovenol(+)-2′,5′-Dimethylphenylcarbamoyl-physovenol(+)-2′,6′-Dimethylphenylcarbamoyl-physovenol(+)3′,4′-Dimethylphenylcarbamoyl-physovenol(+)-3′,5′-Dimethylphenylcarbamoyl-physovenol(+)-3′,6′-Dimethylphenylcarbamoyl-physovenol(+)-2′,4′,6′-Trimethylphenylcarbamoyl-physovenl


 






555657585960616263646566676869707172
H2′-CH33′-CH34′-CH32′-CH2CH33′-CH2CH34′-CH2CH32′-CH2(CH3)23′-CH2(CH3)24′-CH2(CH3)22′,3′-CH32′,4′-CH32′,5′-CH32′,6′-CH33+,4′-CH33′,5′-CH33′,6′-CH32′,4′,6′-CH3
(+)-Phenylcarbamoyl-thiaphysavenol(+)-2′-Methylphenylcaxbamoyl-thiaphysovenol(+)-3′-Methylphenylcarbamoyl-thiaphysovenol(+)-4′-Methyl phenylcarbamoyl-thiaphysovenol(+)-2′-Ethylphenylvarbamoyl-thiaphysovenal(+)-3′-Eethylphenylcarbamoyl-thiaphysovenol(+)-4′-Eethylphenylcarbamoyl-thiaphysovenol(+)-2′-Isopropylphenylcarbamoyl-thiaphysovenol(+)-3′-Isopropylphenylcarbamoyl-thiaphysovenol(+)-4′-Isopropylphenylcarbamoyl-thiaphysovenol(+)-2′,3′-Dimethylphenylcarbamoyl-thiaphysovenol(+)-2′,4′-Dimethylphenylcarbamoyl-thiaphysovenol(+)-2′,5′-Dimethylphenylcarbamoyl-thiaphysovenol(+)-2′,6′-Dimethylphenylcarbamoyl-thiaphysovenol(+)-3′,4′-Dimethylphenylcarbamoyl-thiaphysovenol(+)-3′,5′-Dimethylphenylcarbamoyl-thiaphysovenol(+)-3′,6′-Dimethylphenylcarbamoyl-thiaphysovenol(+)-2′,4′,6′-Trimethylphenylcarbamoyl-thiaphysovenol


 






73747576
CH3phenyl2′-tolyl4′-cymyl
(+)-N1-Allylnorphysostigmine(+)-N1-Allylnorphenserine(+)-N1-Allylnortolserine(+)-N1-Allylnorcymserine


 






77787980
CH3phenyl2′-tolyl4′-cymyl
(+)-N1-Phenethylnorphysostigmine(+)-N1-Phenethylnorphenserine(+)-N1-Phenethylnortolserine(+)-N1-Phenethylnorcymserine


 






81828384
CH3phenyl2′-tolyl4′-cymyl
(+)-3-(1-Methylamino)-ethyl-2H-indol-5yl-methylcarbamate(+)-3-(1-Methylamino)-ethyl-2H-indol-5yl-phenylcarbamate(+)-3-(1-Methylamino)-ethyl-2H-indol-5yl-tolylcarbamate(+)-3-(1-Methylamino)-ethyl-2H-indol-5yl-cymylcarbamate


 






85868788
CH3phenyl2′-tolyl4′-cymyl
(+)-N1-Benzylnorphysostigmine(+)-N1-Benzylnorphenserine(+)-N1-Benzylnortolserine(+)-N1-Benzylnorcymserine


 






89909192
CH3phenyl2′-tolyl4′-cymyl
(+)-N1-Norphysostigmine(+)-N1-Norphenserine(+)-N1-Nortolserine(+)-N1-Nororcymserine


 






93949596
CH3phenyl2′-tolyl4′-cymyl
(+)-N8-Benzylnorphysostigmine(+)-N8-Benzylnorphenserine(+)-N8-Benzylnortolserine(+)-N8-Benzylnorcymserine


 






979899100 
CH3phenyl2′-tolyl4′-cymyl
(+)-N8-Norphysostigmine(+)-N8-Norphenserine(+)-N8-Nortolserine(+)-N8-Norcymserine


 






101 102 103 104 
CH3phenyl2′-tolyl4′-cymyl
(+)-N1,N8-Bisbenzylnorphysostigmine(+)-N1,N8-Bisbenzylnorphenserine(+)-N1,N8-Bisbenzylnortolserine(+)-N1,N8-Bisbenzylnarcymserine


 






105 106 107 108 
CH3phenyl2′-tolyl4′-cymyl
(+)-N1,N8-Bisnorphysostigmine(+)-N1,N8-Bisnorphenserine(+)-N1,N8-Bisnortolserine(+)-N1,N8-Bisnorcymserine


 






109 110 111 112 
CH3phenyl2′-tolyl4′-cymyl
(+)-3a-Phenylphysostigmine(+)-3a-Phenylphenserine(+)-3a-Phenyltolserine(+)-3a-Phenylcymserine









The invention, in one aspect, a compound having the formula XIV or XV




    
    
        wherein R1 and R2 are, independently, hydrogen, branched or straight chain C1–C8 alkyl, substituted or unsubstituted aryl, or aralkyl;
        R3 is branched or straight chain C1–C4 alkyl or heteroalkyl or C4–C8 alkyl or heteroakyl, or substituted or unsubstituted aryl;
        X and Y are, independently, O, S, alkyl, hydrocarbon moiety, C(H)R4, or NR5, wherein R4 and R5 are, independently, hydrogen, oxygen, branched or straight chain C1–C8 alkyl, C2–C8 alkenyl, or C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl; and
        R6 is hydrogen; C1–C8 alkyl, C2–C8 alkenyl, C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl, or (CH2)nR7, where R7 is hydroxy, alkoxy, cyano, ester, carboxylic acid, substituted or unsubstituted amino, and n is from 1 to 4,
        wherein the compound having the formula XIV or XV is a racemic mixture or the substantially pure (−)-enantiomer.
    
    


In one embodiment, the compounds having the structure XIV and XV have an enantiomeric purity for the (−)-enantiomer of from 55 to 100%, desirably from 75 to 100%, more desirably from 85 to 100%, more desirably from 95 to 100%, and even more desirably 100%.
In another embodiment, when the compound has the structure XIV or XV, Y is C(H)R4or X is O, S, or C(H)R4.
In yet another embodiment, the compound of formula XIV is MES9280 (FIG. 13K), MES9232 (FIG. 13J), MES9313 (FIG. 13F), or a (+)-isomer or a racemic mixture thereof.
In another embodiment, when the compound has the formula XIV, where X and Y are nitrogen, and the compound is the substantially pure (−)-enantiomer, then R3 is not methyl. In another embodiment, when the compound has the formula XIV, where X is nitrogen, Y is nitrogen or oxygen, and the compound is the substantially pure (−)-enantiomer, then R5 is not hydrogen or C1–C10 alkyl. In another embodiment, when the compound has the formula XIV, where X is nitrogen and Y is sulfur, and the compound is the substantially pure (−)-enantiomer, then R3 is not methyl. In another embodiment, the compound is not (−)-phenserine, (−)-physostigmine, (−)-heptyl-physostigmine, (−)-physovenine, (−)-N(1)-norphysostigmine, MES9217 (FIG. 13H), MES9299 (FIG. 13L), and MES9329 (FIG. 13M). In one embodiment, when the compound is formula XIV or XV, R3 is not methyl. In particular embodiments, R3 is a branched or straight chain alkyl or heteroalkyl group of 2, 3, 4, 5, 6, 7, or 8 carbons or substituted or unsubstituted aryl.
FIGS. 1 and 2 show one approach to the synthesis of compounds having the structure I and II. Using techniques known in the art (see Julian et al., J. Am. Chem. Soc., 1935, 57, 563; Lee et al., J. Org. Chem., 1991, 56, 872; Pei et al., Heterocycles, 1995, 41, 2823; Lee et al., J. Chromatography, 1990, 523, 317; and Pei et al., Heterocycles, 1994, 39, 557, which are incorporated by reference in their entirety), the physostigmine compound A (FIG. 1) can be prepared in high yield and enantiopurity. Compound A is an important intermediate, and can be used to produce a variety of compounds having the structure I and II. FIG. 2 shows that by using techniques known in the art (see Yu et al., Heterocycles, 1988, 27, 745; Yu et al., Helv. Chem. Res., 1991, 74, 761; He et al., Med. Chem. Res., 1992, 2, 229; Pei et al., Med. Chem. Res., 1995, 5, 455; Yu et al., J. Med. Chem., 1998, 31, 2297; Zhu, Tet. Lett., 2000, 41, 4861; Pei et al., Med. Chem. Res., 1995, 5, 455; and Yu et al., J. Med. Chem., 1997, 40, 2895, which are incorporated by reference in their entirety) compound A can be converted to a number of different compounds having the structure I and II. The numbers below each compound in FIG. 2 correspond to the compound numbers in Table 1. The racemic mixture of I and II as well as compounds XIV and XV can be prepared using the synthetic procedure outlined in FIGS. 1 and 2, where a chiral chromatography step is not performed to produce the racemic mixture or the (−)-enantiomer is isolated instead of the (+)-enantiomer.
The invention also relates to a compound having the formula V, VI, or VII




    
    
        wherein R8 is hydrogen, branched or straight chain C1–C8 alkyl, substituted or unsubstituted aryl, aralkyl, or CR9R10OR11, where R9 and R10 are, independently, hydrogen or alkyl, and R11 is alkyl;
        R3 is branched or straight chain C1–C4 alkyl, or substituted or unsubstituted aryl;
        X and Y are, independently, O, S, C(H)R4, or NR5, wherein R4 and R5 are, independently, hydrogen, oxygen, branched or straight chain C1–C8 alkyl, C2–C8 alkenyl, or C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl; and
        R6 is hydrogen; C1–C8 alkyl, C2–C8 alkenyl, C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl, or (CH2)nR7, where R7 is hydroxy, alkoxy, cyano, ester, carboxylic acid, substituted or unsubstituted amino, and n is from 1 to 4,
        with the proviso that when the compound is formula V, X is NCH3, and Y is NR5, where R5 is hydrogen, loweralkyl, arylloweralkyl, heteroarylloweralkyl, cycloalkylmethyl, or loweralkenyl methyl, R3 is not methyl or R8 is not H or loweralkyl.
    
    


The compounds having the formula V–VII as well as compounds VIII–XIII described below are referred to herein as “non-carbamate compounds” because they do not possess the carbamate group present in compounds I–IV, XIV–XVI. Compounds V–XIII are generally the synthetic precursors to compounds I–IV. FIG. 1 provides a general synthesis to intermediate A, which is a species of compound V. Intermediate A is shown as the (+)-enantiomer; however, the (−)-enantiomer can also be isolated using chiral chromatography. Similarly, the (+)- and (−)-enantiomers of compound VI can also be produced using similar methodolgy. Furthermore, in the absence of the chiral chromatography step in FIG. 1, the racemic compounds V and VI can be produced.
In another embodiment, when the non-carbamate compound has the formula VII, where X is NR5, the reaction depicted in Scheme 1 can be used to produce the compounds. For example, in Scheme I, compound B (compound VII where X is NH) can be treated with a base, such as NaNH2, then treated with an alkyl or aralkyl halide compound to produce compound C.




In one embodiment, when the compound is formula V, X and Y are NR5, wherein R5 is branched or straight chain C1–C8 alkyl, desirably methyl. In another embodiment, when the compound is formula V, R3 is methyl, X and Y are NCH3, and R8 is C1–C8 straight chain alkyl, desirably methyl.
In another embodiment, when the compound is formula VII, X is NR5, wherein R5 is branched or straight chain C1–C8 alkyl or aralkyl, desirably benzyl. In another embodiment, when the compound is formula VII, R6 is (CH2)nR7, where R7 is a substituted or unsubstituted amino group. In another embodiment, when the compound is formula VII, X is NR5, where R5 is benzyl, R6 is (CH2)2N(CH3)2, and R8 is methyl.
The invention also relates to a compound having the formula VIII




wherein R8 is hydrogen, branched or straight chain C1–C8 alkyl, substituted or unsubstituted aryl, aralkyl, or CR9R10OR11, where R9 and R10 are, independently, hydrogen or alkyl, and R11 is alkyl;

    R3 is branched or straight chain C1–C4 alkyl, or substituted or unsubstituted aryl;
    X is O, S, C(H)R4, or NR5, wherein R4 and R5 are, independently, hydrogen, oxygen, branched or straight chain C1–C8 alkyl, C2–C8 alkenyl, or C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl; and
    R6 is hydrogen; C1–C8 alkyl, C2–C8 alkenyl, C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl, or (CH2)nR7, where R7 is hydroxy, alkoxy, cyano, ester, carboxylic acid, substituted or unsubstituted amino, and n is from 1 to 4.


The invention further relates to a compound having the formula IX




wherein R8 is hydrogen, branched or straight chain C1–C8 alkyl, substituted or unsubstituted aryl, aralkyl, or CR9R10OR11, where R9 and R10 are, independently, hydrogen or alkyl, and R11 is alkyl;

    R3 is branched or straight chain C1–C4 alkyl, or substituted or unsubstituted aryl;
    R6 is hydrogen; C1–C8 alkyl, C2–C8 alkenyl, C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl, or (CH2)nR7, where R7 is hydroxy, alkoxy, cyano, ester, carboxylic acid, substituted or unsubstituted amino, and n is from 1 to 4;
    X is O, S, C(H)R4, or NR5, wherein R4 and R5 are, independently, hydrogen, oxygen, branched or straight chain C1–C8 alkyl, C2–C8 alkenyl, or C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl; and
    A, D, and E are, independently, hydrogen, hydroxy, alkoxy, halide, alkyl, aralkyl, or ammo.


The invention also relates to a compound having the formula X




    wherein R8 is hydrogen, branched or straight chain C1–C8 alkyl, substituted or unsubstituted aryl, aralkyl, or CR9R10R11, where R9 and R10 are, independently, hydrogen or alkyl, and R11 is alkyl;
    R6 is hydrogen; C1–C8 alkyl, C2–C8 alkenyl, C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl, or (CH2)nR7, where R7 is hydroxy, alkoxy, cyano, ester, carboxylic acid, substituted or unsubstituted amino, and n is from 1 to 4;
    X is O, S, C(H)R4, or NR5, wherein R4 and R5 are, independently, hydrogen, oxygen, branched or straight chain C1–C8 alkyl, C2–C8 alkenyl, or C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl; and
    Z is halide, hydroxy, or alkoxy.


The invention also relates to a compound having the formula XI




    wherein R8 is hydrogen, branched or straight chain C1–C8 alkyl, substituted or unsubstituted aryl, aralkyl, or CR9R10OR11, where R9 and R10 are, independently, hydrogen or alkyl, and R11 is alkyl;
    R6 is hydrogen; C1–C8 alkyl, C2–C8 alkenyl, C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl, or (CH2)nR7, where R7 is hydroxy, alkoxy, cyano, ester, carboxylic acid, substituted or unsubstituted amino, and n is from 1 to 4;
    X is O, S, C(H)R4, or NR5, wherein R4 and R5 are, independently, hydrogen, oxygen, branched or straight chain C1–C8 alkyl, C2–C8 alkenyl, or C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl;
    A, D, E, and G are, independently, hydrogen, hydroxy, alkoxy, halide, alkyl, aralkyl, or amino; and
    Z is halide, hydroxy, or alkoxy.


In one embodiment, steps 3–5 in FIG. 1 can be used to prepare compounds having the formula VIII–XI.
The invention further relates to a compound having the formula XII




    wherein R8 is hydrogen, branched or straight chain C1–C8 alkyl, substituted or unsubstituted aryl, aralkyl, or CR9R10OR11, where R9 and R10 are, independently, hydrogen or alkyl, and R11 is alkyl;
    R6 is hydrogen; C1–C8 alkyl, C2–C8 alkenyl, C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl, or (CH2)nR7, where R7 is hydroxy, alkoxy, cyano, ester, carboxylic acid, substituted or unsubstituted amino, and n is from 1 to 4; and
    R12 and R13 are, independently, hydrogen; C1–C8 alkyl; aryl or substituted aryl; aralkyl; or (CH2)nR14, wherein R14 is hydroxy, alkoxy, ester, carboxylic acid, substituted or unsubstituted amino, and n is from 1 to 4.


The invention also relates to compound having the formula XIII




    wherein R8 is hydrogen, branched or straight chain C1–C8 alkyl, substituted or unsubstituted aryl, aralkyl, or CR9R10OR11, where R9 and R10 are, independently, hydrogen or alkyl, and R11 is alkyl;
    R6 is hydrogen; C1–C8 alkyl, C2–C8 alkenyl, C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl, or (CH2)nR7, where R7 is hydroxy, alkoxy, cyano, ester, carboxylic acid, substituted or unsubstituted amino, and n is from 1 to 4; and
    R12 and R13 are, independently, hydrogen; C1–C8 alkyl; aryl or substituted aryl; aralkyl; or (CH2)nR14, wherein R14 is hydroxy, alkoxy, ester, carboxylic acid, substituted or unsubstituted amino, and n is from 1 to 4; and
    A, D, E, and G are, independently, hydrogen, hydroxy, alkoxy, halide, alkyl, aralkyl, or amino.


In one embodiment, compounds having the formula XII and XIII can be prepared by the general reaction shown in Scheme 2.




The invention also relates to a compound having the formula XVI




    
    
        wherein R1 and R2 are, independently, hydrogen, branched or straight chain C1–C8 alkyl, substituted or unsubstituted aryl, or aralkyl;
        R3 is branched or straight chain C1–C4 alkyl, or substituted or unsubstituted aryl;
        R5 and R15 are, independently, hydrogen, oxygen, branched or straight chain C1–C8 alkyl, C2–C8 alkenyl, or C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl; and
        wherein the compound is a racemic mixture, the substantially pure (−)-enantiomer, or the substantially pure (+)-enantiomer.
    
    


In a related aspect, the invention features the compound disclosed in FIG. 13C (MES9287) and MES9286 (Table 2).
Additional non-carbamate compounds of the present invention are shown in Table 2.







TABLE 2







9206








 


9201








 


9199








 


9191








 


9226








 


9205








 


9203








 


9202








 


9230








 


9225








 


9222








 


9215








 


9236








 


9229








 


9228








 


9227








 


9243








 


9235








 


9234








 


9231








 


9266








 


9242








 


9238








 


9237








 


9275








 


9260








 


9259








 


9257








 


9279








 


9271








 


9270








 


9276








 


9295








 


9278








 


9277








 


9276








 


9305








 


9293








 


9292








 


9291








 


9311








 


9302








 


9301








 


9295








 


9317








 


9310








 


9309








 


9306








 


9234








 


9316








 


9314








 


9312








 


9323








 


9321








 


9319








 


9318








 


9320








 


9330








 


9335








 


9331








 


9286








 


9287








 


9290















In one embodiment, the present invention provides a method of inhibiting production of amyloid precursor protein in a cell, comprising contacting the cell with a compound having the formula I–XVI and any combination thereof. As used herein, “inhibiting” means decreasing the amount or concentration of amyloid precursor protein. “inhibition” also refers to halting or reducing the production of amyloid protein precursor, wherein the concentration of amyloid protein precursor is reduced. Thus, the inhibition of production of amyloid precursor protein can be measured, for example, by comparing the amount of amyloid precursor protein produced by cells after contacting the cells with the compound having the formula I–XIII and any combination thereof, with the amount of amyloid precursor protein produced by control cells that have not been contacted with a compound having the formula I–XIII and any combination thereof. In one embodiment, the cell that is contacted with the compound is in vivo, ex vivo, or in vitro. The cell of this invention can be a mammalian cell, desirably a human cell.
In a desirable embodiment, the compounds inhibit production of amyloid precursor protein, Aβ1-40, and/or Aβ1-42 in a cell or a mammal by at least 30, 50, 60, 70, 80, 90, 95, or 100% compared to a buffer control, as measured using standard assays such as those described herein. In another desirable embodiment, the compound inhibits production of amyloid precursor protein, Aβ1-40, and/or Aβ1-42 in a cell or a mammal by at least 2, 5, 10, 20, or 50-fold compared to a buffer control, as measured using standard assays such as those described herein.
As used herein, “contacting” means exposure of at least one cell to a compound of the present invention. The cell of this invention can be, but is not limited to, a neural cell or supporting cell (e.g., glial or astrocyte). The term “neural cell” is defined as any cell that can be located in the central or peripheral nervous system or is a precursor or derivative thereof, including, for example, but not limited to, neuronal cells, glial cells, neural stem cells, neuronal stem cells and neuroblasts. The cell can be contacted in vitro with the compound, for example, by adding the compound to the culture medium (by continuous infusion, by bolus delivery, or by changing the medium to a medium that contains the compound), or the cell can be contacted with the compound in vivo (e.g., by local delivery, systemic delivery, intravenous injection, bolus delivery, or continuous infusion). In vitro contact may be preferred, for example, for measuring the effect of the compound on a population of cells. In vivo contact would be employed for inhibiting production of amyloid precursor protein in a subject in need of such inhibition, (e.g., a subject) with a neurodegenerative disease, for example, Alzheimer's Disease.
The subject of this invention can be any mammal that produces amyloid precursor protein, such as a primate and more desirably, a human. The subject of this invention can also be domesticated animals, such as cats, dogs, etc., livestock (e.g., cattle, horses, pigs, sheep, goats, etc.), and laboratory animals (e.g., mouse, rabbit, rat, guinea pig, etc.).
The duration of contact with a cell or population of cells is determined by the time the compound is present at physiologically effective levels or at presumed physiologically effective levels in the medium or extracellular fluid bathing the cell or cells. Desirably, the duration of contact is 1–48 hours and, more desirably, for 24 hours, but such time would vary based on the half-life of the compound and could be optimized by one skilled in the art using routine experimentation.
Examples of compounds used in the methods of this invention for inhibiting amyloid protein precursor include, but are not limited to, (+)-phenserine, (+)-cymserine, (+)-N1-phenethylnorcymserine, or (+)-N1,N8-bisnorcymserine. In a desired embodiment, the compound is (+)-phenserine, compound V, wherein X and Y are NCH3, R3 and R8 are methyl, and the compound is the substantially pure (+)-enantiomer, or compound VII, wherein X is NR5, where R5 is benzyl, R6 is (CH2)2N(CH3)2, and R8 is methyl.
In another embodiment, the present invention also provides a method of inhibiting production of amyloid precursor protein in a subject, comprising administering to the subject an effective amount of a compound having the structure I–XVI and any combination thereof in a pharmaceutically acceptable canier, whereby the compound inhibits production of amyloid precursor protein in the subject.
In a desirable embodiment, the compounds of the invention inhibit production of amyloid precursor protein, Aβ1-40, and/or Aβ1-42 in the subject or in a sample from the subject by at least 30, 50, 60, 70, 80, 90, 95, or 100% compared to a buffer control, as measured using standard assays such as those described herein. In another desirable embodiment, the compound inhibits production of amyloid precursor protein, Aβ1-40, and/or Aβ1-42 in the subject or in a sample from the subject by at least 2, 5, 10, 20, or 50-fold compared to a buffer control, as measured using standard assays such as those described herein.
The compounds of the present invention can be administered in vivo to a subject in need thereof by commonly employed methods for administering compounds in such a way to bring the compound in contact with cells. The compounds of the present invention can be administered orally, parenterally, transdermally, extracorporeally, topically or the like, although oral or parenteral administration is typically desired. Parenteral administration of the compounds of the present invention, if used, is generally characterized by injection. Injectables can be prepared in conventional forms, either as liquid solutions or suspensions, solid forms suitable for solution of suspension in liquid prior to injection, or as emulsions. As used herein, “parenteral administration” includes intradermal, subcutaneous, intramuscular, intraperitoneal, intravenous, intra-articular and intratracheal routes. Additionally, the compound can be administered via a slow release or sustained release system such that a constant dosage is maintained. See, e.g., U.S. Pat. No. 3,610,795, which is incorporated by reference herein in its entirety. The compounds can also be administered using polymer based delivery systems, including, for example, microencapsulation, which techniques are well known in the art.
The dosage of the compound varies depending on the weight, age, sex and condition of the subject as well as the method and route of administration. As an example, the dosage of the compound is from about 0.1 mg/kg to about 100 mg/kg of body weight. The lower limit for the dosage can be about 0.1, 0.5, 1, 2, 5, 10, 15, 20, 25, 30, or 40 mg/kg and the upper limit can be about 10, 15, 20, 25, 30, 40, 50, 60, 70, 80, 90, or 100 mg/kg. Any lower limit can be used with any upper limit. More desirably, the compound is administered in vivo in an amount of about 1 to about 20 mg/kg. Thus, an administration regimen could include long-term, daily treatment. By “long-term” is meant at least two weeks and, desirably, several weeks, months, or years of duration. Necessary modifications in this dosage range may be determined by one of ordinary skill in the art using only routine experimentation given the teachings herein. See Remington's Pharmaceutical Sciences (Martin, E. W., ed., latest edition), Mack Publishing Co., Easton, Pa. The dosage can also be adjusted by the individual physician in the event of any complication.
The compounds can be administered conventionally as compositions containing the active compound as a predetermined quantity of active material calculated to produce the desired therapeutic effect in association with the required diluent (i.e., carrier or vehicle). Depending on the intended mode of administration, the compound can be in pharmaceutical compositions in the form of solid, semi-solid or liquid dosage forms, such as, for example, tablets, suppositories, pills, capsules, powders, liquids, suspensions, lotions, creams, gels, or the like, desirably in unit dosage form suitable for single administration of a precise dosage. The compositions will include, as noted above, an effective amount of the selected compound in combination with a pharmaceutically acceptable carrier and, in addition, may include other medicinal compounds, pharmaceutical compounds, carriers, adjuvants, diluents, etc. By “pharmaceutically acceptable” is meant a material that is not biologically or otherwise undesirable, i.e., the material may be administered to an individual along with the selected compound without causing any undesirable biological effects or interacting in a deleterious manner with any of the other components of the pharmaceutical composition in which it is contained.
For solid compositions, conventional nontoxic solid carriers include, for example, pharmaceutical grades of mannitol, lactose, starch, magnesium stearate, sodium saccharin, talc, cellulose, glucose, sucrose, magnesium carbonate, and the like. Liquid pharmaceutically administrable compositions can, for example, be prepared by dissolving, dispersing, etc. an active compound as described herein and optional pharmaceutical adjuvants in an excipient, such as, for example, water, saline, aqueous dextrose, glycerol, ethanol, and the like, to thereby form a solution or suspension. If desired, the pharmaceutical composition to be administered may also contain minor amounts of nontoxic auxiliary substances such as wetting or emulsifying compounds, pH buffering compounds and the like, for example, sodium acetate, sorbitan monolaurate, triethanolamine sodium acetate, triethanolamine oleate, etc. Thus, the compositions are administered in a manner compatible with the dosage formulation and in a therapeutically effective amount. As discussed above, precise amounts of active ingredient required to be administered depend on the judgment of the practitioner and are peculiar to each individual.
For oral administration, fine powders or granules may contain diluting, dispersing, and/or surface active compounds, and may be presented in water or in a syrup, in capsules or sachets in the dry state, or in a nonaqueous solution or suspension wherein suspending compounds may be included, in tablets wherein binders and lubricants may be included, or in a suspension in water or a syrup. Where desirable or necessary, flavoring, preserving, suspending, thickening, or emulsifying compounds may be included. Tablets and granules are desired oral administration forms, and these may be coated.
In another embodiment, the present invention provides a method of treating a disorder associated with abnormal production of amyloid precursor protein, such as, for example, dementia in a subject, comprising administering to the subject an effective amount of the compound having the formula I–XVI and any combination thereof in a pharmaceutically acceptable carrier, whereby the compound treats the disorder in the subject. As used herein, the term “dementia” describes a neurodegenerative disorder that results from an organic brain disease in which a subject experiences usually irreversible deterioration of intellectual faculties with accompanying emotional disturbances. An example of dementia includes, but is not limited to, Alzheimer's disease. An example of another disorder that can be treated by the methods of this invention includes, but is not limited to, cerebral amyloidosis. In a desirable embodiment, a compound used for the treatment of dementia improves a symptom associated with dementia or Alzheimer's, stabilizes a symptom, or delays the worsening of a symptom In other desirable embodiments, the compound increases the life-span of a subject compared to the average life-span of corresponding subjects not administered the compound. In yet other desirable embodiments, the compound is used to prevent or delay the onset of dementia or Alzheimer's.
In general, an “effective amount” of a compound is that amount needed to achieve the desired result or results. Thus, for example, administering to a subject (e.g., a human) with Alzheimer's disease an effective amount of a compound of the present invention can result in slowing, stopping, or even possibly reversing the deterioration of the subject's intellectual faculties and other accompanying neurological signs and symptoms. Therefore, the inhibition of the production of amyloid precursor protein, by the methods of the present invention, treats the subject with Alzheimer's disease. The effective amount of the compound needed to treat dementia is from about 0.5 mg to about 200 mg. The lower limit for the effective amount of the compound can be about 0.5, 1, 2, 5, 10, 20, 30, 40, 50, 100, or 150 mg, and the upper limit can be about 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, or 200 mg. Any lower limit can be used with any upper limit. In one embodiment, when the subject is a human, the effective amount of compound to treat dementia is from about 0.5 to about 100 mg. In another embodiment, (+)-phenserine, (+)-cymserine, (+)-N1-phenethylnorcymserine, (+)-N1,N8-bisnorcymserine, compound V, wherein X and Y are NCH3, R3 and R8 are methyl, and the compound is the substantially pure (+)-enantiomer, or compound VII, wherein X is NR5, where R5 is benzyl, R6 is (CH2)2N(CH3)2, and R8 is methyl can be used in these amounts to treat dementia. In a desired embodiment, (+)-phenserine can be used in these amounts to treat dementia in a subject.
In a further embodiment, the present invention relates to a method of binding an amyloid precursor protein messenger RNA 5′ untranslated region (5′UTR) in a cell, comprising contacting the cell with a compound having the formula I–XVI and any combination thereof, whereby the compound binds the amyloid precursor protein messenger RNA 5′ untranslated region in the cell, thereby inhibiting amyloid protein production. The amyloid precursor protein messenger RNA 5′UTR confers translational control of βAPP protein synthesis. In one embodiment, (+)-phenserine, (+)-cymserine, (+)-N1-phenethylnorcymserine, (+)-N1,N8-bisnorcymserine, compound V, wherein X and Y are NCH3, R3 and R8 are methyl, and the compound is the substantially pure (+)-enantiomer, or compound VII, wherein X is NR5, where R5 is benzyl, R6 is (CH2)2N(CH3)2, and R8 is methyl can be used to bind the amyloid precursor protein with messenger RNA 5′ UTR. In a desired embodiment, (+)-phenserine can be used to bind to an amyloid precursor protein messenger RNA 5′ untranslated region in a cell. In a desirable embodiment, at least 30, 50, 60, 70, 80, 90, 95, or 100% of the amyloid precursor protein mRNA in a cell is bound by the compound.
In a further embodiment, the present invention relates to a method of inhibiting translation of an amyloid precursor protein messenger RNA in a cell, comprising contacting the cell with a compound having the formula I–XVI and any combination thereof, whereby the compound binds the amyloid precursor protein messenger RNA 5′ and/or 3′ untranslated region in the cell, or binds a protein that interacts with the amyloid precursor protein messenger RNA 5′ and/or 3′ untranslated region in the cell, or alters a process, either indirectly or directly, such as glycosylation or phosphorylation that then changes the binding of a specific regulatory protein to amyloid precursor protein messenger RNA 5′ and/or 3′ untranslated region in the cell, thereby inhibiting amyloid protein production. In one embodiment, (+)-phenserine, (+)-cymserine, (+)-N1-phenethylnorcymserine, (+)-N1,N8-bisnorcymserine, compound V, wherein X and Y are NCH3, R3 and R8 are methyl, and the compound is the substantially pure (+)-enantiomer, or compound VII, wherein X is NR5, where R5 is benzyl, R6 is (CH2)2N(CH3)2, and R8 is methyl can be used to inhibit the translation of the amyloid precursor protein with messenger RNA. In a desired embodiment, (+)-phenserine can be used to inhibit the translation of the amyloid precursor protein messenger RNA by interfering with the post-transcriptional regulation of the amyloid precursor protein RNA in a cell. In a desirable embodiment, the compound inhibits production of amyloid precursor protein, Aβ1-40, and/or Aβ1-42 by at least 30, 50, 60, 70, 80, 90, 95, or 100% compared to a buffer control as measured using standard assays such as those described herein. In another desirable embodiment, the compound inhibits production of amyloid precursor protein, Aβ1-40, and/or Aβ1-42 by at least 2, 5, 10, 20, or 50-fold compared to a buffer control, as measured using standard assays such as those described herein.
The present invention also provides a method of screening a compound for the ability to inhibit production of amyloid precursor protein, Aβ1-40, and/or Aβ1-42, comprising (a) contacting the cell with the compound having the formula I–XVI and any combination thereof, and (b) detecting a decrease in amyloid precursor protein, Aβ1-40, and/or Aβ1-42 production in a cell contacted with the compound as compared to the amount of amyloid precursor protein, Aβ1-40, and/or Aβ1-42 production in a control cell not contacted with the compound, whereby decreased production of amyloid precursor protein, Aβ1-40, and/or Aβ1-42 in the cell identifies the compound as having the ability to inhibit the production of amyloid precursor protein, Aβ1-40, and/or Aβ1-42 in a cell. As shown in the Examples section below, a person of skill in the art can measure the amount of βAPP, Aβ1-40, and/or Aβ1-42 production in a control population of cells and compare the production of βAPP, Aβ1-40, and/or Aβ1-42 in a population of cells contacted with a compound to be screened by the methods of the present invention. A decrease in the production of βAPP, Aβ1-40, and/or Aβ1-42 in a population of cells contacted with a compound as compared to the production of βAPP, Aβ1-40, and/or Aβ1-42 in a population of control cells identifies the compound as having the ability to inhibit production of amyloid precursor protein, Aβ1-40, and/or Aβ1-42. In a desirable embodiment, the compound inhibits production of amyloid precursor protein, Aβ1-40, and/or Aβ1-42 by at least 30, 50, 60, 70, 80, 90, 95, or 100% compared to a buffer control, as measured using standard assays such as those described herein. In another desirable embodiment, the compound inhibits production of amyloid precursor protein, Aβ1-40, and/or Aβ1-42 by at least 2, 5, 10, 20, or 50-fold compared to a buffer control, as measured using standard assays such as those described herein.
The present invention further provides a method of screening a compound for the ability to inhibit amyloid precursor protein production by binding an amyloid precursor protein messenger RNA 5′ untranslated region, comprising (a) contacting the messenger RNA with the compound; (b) detecting the binding of the compound to the amyloid precursor protein-messenger RNA 5′ untranslated region; and (c) detecting the inhibition of amyloid precursor protein production from an amyloid precursor protein-messenger RNA 5′ untranslated region, thereby identifying a compound having the ability to inhibit amyloid precursor protein messenger RNA 5′ untranslated region. The binding of the compound to the amyloid precursor protein messenger RNA 5′ untranslated region inhibits β amyloid precursor protein (βAPP) from the messenger RNA by directly preventing the binding of the ribosomal translational subunit with the mRNA through steric hindrance. The detection of binding of a compound to the 5′ UTR of the amyloid precursor protein messenger RNA can be carried out by methods standard in the art for detecting the binding of substances to nucleic acids such as RNA. The detection of inhibition of amyloid precursor protein production upon contact with the compound can be carried out by the methods provided in the Examples herein, as well as protocols well known in the art. The messenger RNA can be in a cell or in a cell-free environment (e.g., a cell-free translation system). In a desirable embodiment, at least 30, 50, 60, 70, 80, 90, 95, or 100% of the amyloid precursor protein mRNA in a cell is bound by the compound.
The present invention further provides a method of screening a compound for the ability to inhibit amyloid precursor protein, Aβ1-40, and/or Aβ1-42 production by inhibiting translation of the amyloid precursor protein messenger RNA, comprising (a) contacting the cell with the compound; and (b) detecting the inhibition of amyloid precursor protein, Aβ1-40, and/or Aβ1-42 production from an amyloid precursor protein RNA, thereby identifying a compound having the ability to inhibit amyloid precursor protein messenger RNA translation. In another embodiment, the screening method further comprises after step (a) detecting the amount of the amyloid precursor protein-messenger RNA. In one embodiment, the amount of amyloid precursor protein mRNA is not inhibited by the compound or is inhibited by less than 80, 80, 50, 40, 30, 20, or 10%. In this case, the compound primarily or only inhibits the translation of amyloid precursor protein. It is also contemplated that in other embodiments a compound may inhibit both transcription and translation of amyloid precursor protein or inhibit only transcription of amyloid precursor protein. While not meant to limit the invention to any particular mechanism of action, the inhibition of translation by the compound can result from the binding of the compound to the amyloid precursor protein messenger RNA 5′ and/or 3′ untranslated region(s) inhibiting β amyloid precursor protein (βAPP) from the messenger RNA by directly preventing the binding of the ribosomal translational subunit with the mRNA through steric hindrance or by inhibiting the binding of an important regulatory protein, or the binding of the compound to the regulatory protein or by the compound modifying an important regulatory protein such that it no longer can interact with the amyloid precursor protein RNA. The direct detection of binding of a compound to the 5′ and/or 3′ UTR(s) of the amyloid precursor protein messenger RNA can be carried out by methods standard in the art for detecting the binding of substances to nucleic acids such as RNA, such as NMR or mass spectroscopy. The detection of inhibition of amyloid precursor protein production upon contact with the compound can be carried out by the methods provided in the Examples herein, as well as protocols well known in the art. The messenger RNA can be in a cell or in a cell-free environment (e.g., a cell-free translation system). In a desirable embodiment, the compound inhibits production of amyloid precursor protein, Aβ1-40, and/or Aβ1-42 by at least 30, 50, 60, 70, 80, 90, 95, or 100% compared to a buffer control, as measured using standard assays such as those described herein. In another desirable embodiment, the compound inhibits production of amyloid precursor protein, Aβ1-40, and/or Aβ1-42 by at least 2, 5, 10, 20, or 50-fold compared to a buffer control, as measured using standard assays such as those described herein. In yet another desirable embodiment, at least 30, 50, 60, 70, 80, 90, 95, or 100% of the amyloid precursor protein mRNA in a cell is bound by the compound. In still another embodiment, at least 30, 50, 60, 70, 80, 90, 95, or 100% of the compound is directly or indirectly bound to an amyloid precursor protein messenger RNA 5′ or 3′ untranslated region or to an RNA binding protein that interacts with the amyloid precursor protein messenger RNA 5′ or 3′ untranslated region.
In another embodiment, the invention relates to a method of screening a compound for the ability to inhibit amyloid protein production by eliciting a change in reporter gene expression, comprising:

    
    
        (a) contacting a cell transfected with a reporter gene containing the 5′ and/or 3′ UTR(s) of the amyloid precursor protein messenger RNA with the compound, and
        (b) detecting a decrease in reporter gene expression or activity; thereby identifying a compound having the ability to inhibit amyloid precursor protein messenger RNA translation
    
    


In a desirable embodiment, the compound inhibits reporter gene expression or activity by at least 30, 50, 60, 70, 80, 90, 95, or 100% compared to a buffer control, as measured using standard assays such as those described herein. In another desirable embodiment, the compound inhibits reporter gene expression or activity by at least 2, 5, 10, 20, or 50-fold compared to a buffer control, as measured using standard assays such as those described herein.
The compounds used in the screening methods of this invention can be, but is not limited to, a compound having the structure I–XVI and any combination thereof. In various embodiments, the compound is a (+)-isomer, (−)-isomer, or a racemic mixture. In various embodiments of any of the methods of the invention, the compound is a (+)-isomer, (−)-isomer, or a racemic mixture of MES 9295 (FIG. 13E). In other embodiments of any of the methods of the invention, the compound is not a (+)-isomer, (−)-isomer, or a racemic mixture of MES 9295.
In desirable embodiment of any of the aspects of the invention, the compound inhibits cholinesterase activity, such as acetylcholinesterase or butyrylcholinesterase activity, by less than 80, 70, 60, 50, 40, 30, 20, 10, or 5% (in order of increasing preference) relative to a buffer only control. In other desirable embodiments, inhibition of cholinesterase activity, such as acetylcholinesterase or butyrylcholinesterase activity, by the compound is at least 2, 5, 10, 20, 50, or 100-fold less than the inhibition of cholinesterase activity by the corresponding amount of (−)-phenserine. In other desirable embodiments, inhibition of cholinesterase activity, such as acetylcholinesterase or butyrylcholinesterase activity, by a (+) isomer or a racemic mixture is at least 2, 5, 10, 20, 50, or 100-fold less than the inhibition of cholinesterase activity by the corresponding amount of (−)-isomer. In yet other desirable embodiments, the compound is substantially free of cholinesterase inhibitory activity. Inhibition of cholinesterase activity can be measured using any standard assay. For example, the assay and the in vivo mouse model described in U.S. Pat. No. 4,791,107, which is incorporated by reference in its entirety, or the in vivo mouse model described herein can be used.
In other desirable embodiments of any of the aspects of the invention, the compound results in a less than 20, 10, 5, or 2-fold increase in the amount of released lactate dehydrogenase (a marker of cell viability and integrity) relative to the amount of released lactate dehydrogenase in the absence of the compound or in the presence of a buffer control. In still other desirable embodiments, the amount of compound that is administered to a subject per kg body weight of the subject does not cause tremors or death when administered in the in vivo mouse model described herein. In yet other desirable embodiments, less than 80, 70, 60, 50, 40, 30, 20, 10, or 5% of the neuronal cells contacted with the compound are killed by the compound. In another desirable embodiment, the does of the compound is equal to or greater than 1 mg/kg of body weight, 5 mg/ kg, or 10 mg/kg.
In other desirable embodiments of any of the aspects of the invention, the compound inhibits production of amyloid precursor protein, Aβ1-40, and/or Aβ1-42 by at least 30, 50, 60, 70, 80, 90, 95, or 100% compared to a buffer control. In another embodiment, the compound inhibits production of amyloid precursor protein, Aβ1-40, and/or Aβ1-42 by at least 30, 50, 60, 70, 80, 90, 95, or 100% compared to a buffer control, and inhibits cholinesterase activity by less than 80, 70, 60, 50, 40, 30, 20, 10, or 5% relative to a buffer only control. In other desirable embodiments, the compound inhibits intracellular and/or extracellular APP or Aβ production. In yet other desirable embodiments, the compound inhibits production of amyloid precursor protein in a cell or mammal by at least 2, 5, 10, 20, or 50-fold more than it inhibits cholinesterase activity in the cell or mammal. In still other desirable embodiments, the amount of compound required to inhibit production of amyloid precursor protein in a cell or mammal by 50% (IC50 value) is at least 2, 5, 10, 20, 50, or 100-fold less than the amount of compound required to inhibit cholinesterase activity by 50% (IC50 value) in the cell or mammal, as measured using standard assays.
In other desirable embodiments of any of the methods of the invention, the compound is (+)-3,3a,8,8a-Tetrahydro-3a,8-dimethyl-2H-thieno[2,3-b]indole-5-ol methyl ether; (+)-3,3a,8,8a-Tetrahydro-3a,8-dimethyl-2H-thieno[2,3-b]indole-5-ol; (+)-3,3a,8,8a-tetrahydro-3a,8-dimethyl-2H-thieno-[2,3-b]indole-5-ol butyl carbamate; (+),-3,3a,8,8a-tetrahydro-3a,8-dimethyl-2H-thieno[2,3-b]indole-5-ol heptylcarbamate; (+)-3,3a,8,8a-tetrahydro-3a,8-dimethyl-2H-thieno[2,3-b]indole-5-ol phenylcarbamate; (+)-3,3a,8,8a-tetrahydro-3a,8-dimethyl;-2H-thieno[2,3-b]indole-5-ol 2′-methylphenylcarbamate; (+)-3,3a,8,8a-tetrahydro-3a,8-dimethyl-2H-thieno[2,3-b]indole-5-ol 2′ethylphenylcarbamate; (+)-3,3a,8,8a-tetrahydro-3a,8-dimethyl-2H-thieno[2,3-b]indole-5-ol 2′-isopropylphenylcarbamate; (+)-3,3a,8,8a-tetrahydro-3a,8-dimethyl-2H-thieno[2,3-b]indole-5-ol 4′-isopropylphenylcarbamate; (+)-3,3a,8,8a-tetrahydro-3a,8-dimethyl-2H-thieno[2,3-b]indole-5-ol 2′,4′-dimethylphenylcarbamate; (+)-3,3a,8,8a-tetrahydro-3a,8-dimethyl-2H-thieno[2,3-b]indole-5-ol N,N-dimethylcarbamate; (+)-O-methyl-N(1)-noreseroline; (+)-3,3a,8,8a-tetrahydro-3a,8-dimethyl-2H-thieno[2,3-b]indole-5-ol methyl ether; (+)-3,3a,8,8a-tetrahydro-3a,8-dimethyl-2H-thieno[2,3-b]indole-5 ol ((−)-thiaphysovenol); (+)-3,3a,8,8a-Tetrahydro-3a,8-dimethyl-2H-thieno[2,3-b]indole-5-ol 2′,4′-dimethylphenylcarbamate; (+)-3,3a,8,8a-tetrahydro-3a,8-dimethyl-2H-thieno[2,3-b]indole-5-ol 2′-methylphenylcarbamate; (+)-3,3a,8,8a-tetrahydro-3a,8-dimethyl-2H-thieno[2,3-b]indole-5-ol 4′-isopropylphenylcarbamate; (+)-3,3a,8,8a-tetrahydro-3a,8-dimethyl-2H-thieno[2,3-b]indole-5-ol 4′-isopropylphenylcarbamate, or a mixture of the (+)- and (−)-enantiomers thereof (e.g., a racemic mixture). In other embodiments, the compound is the (+)-enantiomer or a mixture of the (+)- and (−)-enantiomers thereof (e.g., a racemic mixture) of a compound disclosed in column 2, line 16 through column 3, line 40 of U.S. Pat. No. 5,378,723 (Brossi et al., issued Jan. 3, 1995).
In other desirable embodiments of any of the methods of the invention, the compound is a compound that falls with the general formula (e.g., column 1, lines 11–52) and or a specifically disclosed compound (e.g., a compound listed in column 21, line 59 through column 38, line 42.) in U.S. Pat. No. 4,791,107 (Hamer et al., issued Dec. 13, 1988), and the compound has negligible cholinesterase inhibitory activity (e.g., causes no detectable cholinesterase inhibition or causes less than 50, 40, 30, 20, 10, or 5% inhibition). In other desirable embodiments, administration of the compound produces no adverse side-effects due to inhibition of cholinesterase activity. These compounds can be the (+)- or (−)-isomers or a mixture thereof (e.g., a racemic mixture). In other embodiments, the compound is a compound that falls with the general formula and or a specifically disclosed compound in U.S. Pat. No. 4,791,107, and the compound is administered in a dose of at least 1 mg/kg, 5 mg/kg, or 10 mg/kg.
In other desirable embodiments of any of the methods of the invention, the compound is a compound that falls with a general formula disclosed in U.S. Pat. No. 5,378,723 (Brossi et al., issued Jan. 3, 1995), U.S. Pat. No. 5,171,750 (Brossi et al., issued Dec. 15, 1992), or U.S. Pat. No. 5,998,460 (Brossi et al., issued Dec. 7, 1999), and the compound has negligible cholinesterase inhibitory activity (e.g., causes no detectable cholinesterase inhibition or causes less than 50, 40, 30, 20, 10, or 5% inhibition) or is administered in a dose of at least 1 mg/kg, 5 mg/kg, or 10 mg/kg. [As an alternative, these structures could be cut and pasted into the application.] In other desirable embodiments of any of the methods of the invention, the compound is the (+)-isomer or a racemic mixture of a compound that falls with a general formula or is specifically disclosed in U.S. Pat. No. 5,378,723, U.S. Pat. No. 5,171,750, or U.S. Pat. No. 5,998,460.
In other embodiments of any of the methods of the invention, the compound is not (−)-phenserine, (−)-physostigmine, (−)-heptyl-physostigmine, (−)-physovenine, (−)-N(1)-norphysostigmine, MES9217 (FIG. 13H), MES9299 (FIG. 13L), or MES9329 (FIG. 13M). In other embodiments of any of the methods of the invention, the compound is not a compound disclosed in U.S. Pat. No. 5,378,723, U.S. Pat. No. 5,171,750, U.S. Pat. No. 5,998,460, or U.S. Pat. No. 4,791,107.
EXAMPLES
The following examples are put forth so as to provide those of ordinary skill in the art with a complete disclosure and description of how the compounds claimed herein are made and evaluated, and are intended to be purely exemplary of the invention and are not intended to limit the scope of what the inventors regard as their invention. Efforts have been made to ensure accuracy with respect to numbers (e.g., amounts, temperature, etc.) but some errors and deviations should be accounted for. Unless indicated otherwise, parts are parts by weight, temperature is in ° C. and is at room temperature, and pressure is at or near atmospheric.
General Considerations
Phenserine: Phenserine is a member of a family of compounds that are phenylcarbamates of hexahydropyrrol indoles with specific side groups that provide it selectivity against either acetyl- or butyryl-cholinesterase, a high brain uptake and a long duration of pharmacological action (Greig et al., 1995; Brossi et al., 1996). The compound was synthesized in its optically (>99.9%) and chemically (>99.9%) pure (−)- and (+)-enantiomeric forms as a tartrate salt, as described (Yu and Brossi, 1988; Greig et al.,1995). The concentration of compound required to inhibit 50% ACBE activity was 22 nM for (−)-phenserine, whereas >25,000 nM was inactive for optically pure (+)-phenserine.
Drug treatment: SK-N-SH neuroblastoma cells were cultured on 60 mm dishes at a concentration of 3×106 cells, and SH-SY-5Y neuroblastoma and U373 astrocytoma cell lines were plated in 100 mm dishes at a concentration of 3×105 cells. The cells were allowed to grow in complete medium (10% FBS, 2 mM glutamine in DMEM) for 3 to 4 days until they reached 70% confluence. To start the experiment, spent medium was removed and replaced with fresh medium (SKNSH—4 ml of DMEM+0.5% FBS; U373—5 ml of DMEM+2.5%FBS) containing 0, 5, or 50 μM phenserine. The cells were incubated at 37° C., 5% CO2 for the specific times indicated. Different media and sera were purchased from Life Technologies (Gaithersberg, Md.).
Inhibitor treatment: One day prior to drug treatment, confluent cultures of U373 cells were pretreated with 25 nM of ERK specific inhibitor, PD98059 (Calbiochem-Novabiochem, La Jolla, Calif.), in 4.5 ml of 2.5% FBS, 2 mM glutamine and DMEM for 16 hours. Phenserine was added to each assay plate and a final volume of 5 ml was reached. To examine for PI 3 kinase involvement, an active 2 μM concentration of the PI 3 kinase inhibitor, LY294002 (Calbiochem-Novabiochem, La Jolla, Calif.), in 4.5 ml of 2.5% FBS, 2 mM glutamine and DMEM was added to each assay plate and incubated for 30 minutes prior to the addition of phenserine. Appropriate vehicle controls were run alongside treated samples.
Lysate preparation: At each time point, the spent medium was collected and stored at −70° C. for later analysis of secretory βAPP levels. The cells were washed twice with PBS, pH 7.4 and incubated on ice for 15 min for lysis with 100 μL of lysis buffer (20 mM HEPES, 2 mM EGTA, 50 mM β-glycophosphate, 1 mM sodium orthovanadate, 1% Triton X-100, 10% glycerol) containing appropriate protease inhibitors (2 mM PMSF, 100 μg/mL aprotinin, 25 μM leupeptin and 20 μg/mL soybean trypsin inhibitor). Each lysate was microcentrifuged for 15 min at 14000 rpm. Protein levels of the supernatant were analyzed by the Bradford protein assay (BioRad, Melville, N.Y.).
Western Blot: Fifteen μg of protein from each sample was mixed with the appropriate volume of 5× Laemmli buffer and boiled for 5 min at 100° C. The samples were loaded onto a 10% NuPAGE Bis-Tris gel in 1× NuPAGE MOPS SDS running buffer (NOVEX, San Diego, Calif.) and the proteins separated at 200 V for 45 min. The gels then were transferred onto nitrocellulose at 25 V for 1.5 h The blots were blocked with 5% non-fat dry milk in 10 mM Tris, pH 8.0 containing 150 mM NaCl for 1 h and washed twice for 15 min in large volumes of TBST (10 mM Tris, pH 8.0, 150 mM NaCl and 0.05% Tween 20). Each blot was probed for 2 h with either 22C11 anti-βAPP-terminal antibody (Boehringer Mannheim, Indianapolis, Ind.), diluted to a concentration of 2.5 μg/mL or anti-activated ERK antibody (Promega, Madison, Wis.), diluted to a concentration of 25 ng/mL. The blots were washed twice for 15 min in TBST and placed in secondary antibody, anti-mouse IgG- or anti-rabbit IgG conjugated to horse radish peroxidase (Sigma, St. Louis, Mo.), for 30 min. Three final TBST washes of 20 min duration each were performed before the samples were detected by chemiluminesence and exposed to film, as per the manufacturer's instructions Amersham Life Science Inc., Arlington Heights, Ill.). Additionally, all blots also were stained with Ponceau S (Sigma, St. Louis, Mo.) to determine equivalent loading of samples. Densitometric quantification of blots was undertaken by using a CD camera and NIH-IMAGE (version 4.1).
Lactate Dehydrogenase Assay: Measurement of released lactate dehydrogenase (LDH) in the conditioned medium was undertaken as a marker of cell viability and integrity, as described previously (Lahiri et al., 1997 and 1998).
Total Aβ Assay: Total Aβ peptide levels in SH-SY-5Y and SK-N-SH cultured samples were assayed by a sensitive ELISA (Suzuki et al., 1994). For total Aβ measurements in conditioned medium, the rabbit polyclonal antibody #3160 (1–40 residues of Aβ) was used as a capture antibody for all species of Aβ peptide (Aβ1–40 and Aβ1–42) while monoclonal antibody 4G8 (17–25 residues of Aβ) was used to detect Aβ peptide levels, and the values were expressed as the mean of six independent assays.
Transfection: One day prior to transfection, U373 cells were plated onto 100 mm dishes at a density of 3×105 cells. On the day of transfection, the cells were given 5 ml of fresh media containing 10% FBS, 2 mM glutaiiine in DMEM. The cells were transfected using a calcium phosphate precipitation method, as per the manufacturer's protocol and described previously (Rogers et al., 1999). Briefly, for each plate, three μg of DNA (5′UTR APP-PSV2-CAT or PSV2-CAT vector) were placed in a final volume of 500 μL of 250 mM CaCl2. The chloramphenicol acetyl transferase (CAT) gene was used as a reporter gene. The DNA solution was slowly pipetted into an aerated, equivalent volume of 2× HeBS, pH 7.05. The resulting precipitate was allowed to stand 10–20 min at RT before its addition to the cells. After 18 h, the medium was changed and the transfected cells were left for two days before drug treatment.
CAT Assay: The cell lysates from transfected U373 cells treated with phenserine were analyzed for their CAT activity using a colorimetric enzyme immunoassay (Boehringer Mannheim, Indianapolis, Ind.). Briefly, 50 μg of protein (an amount previously found to lie within the linear range of the assay) were placed onto anti-CAT coated microtiter plate modules and allowed to bind for 1 h at 37° C. The plates were washed thoroughly after each step. Next, a digoxigenin-labeled anti-CAT antibody was added to the samples and incubated for 1 h at 37° C. A subsequent antibody, anti-digoxigenin conjugated to peroxidase, was placed in the wells for another hour under similar conditions. Finally peroxidase substrate, ABTS, was added to each well and the absorbance of each sample was measured at a wavelength of 405 nm.
Northern Blotting: Total RNA (10 μg) was extracted and prepared from treated astrocytoma cells using an RNA-STAT kit (Tel-test, Friendswood, Tex.). The samples were denatured in formamide, MOPS buffer, formaldehyde, dye mix and ethidium bromide at 65° C. for 10 min, placed on ice for 5 min and electrophoresed on a 1.0% agarose-fromadehyde gel. The gel was blotted onto Hybond Nitrocellulose filters and immobilized by UV crosslinking and heating filters for 2 hours. Each filter was prehybridized in hybridization buffer (1% BSA, 7% SDS, 0.5 M phosphate buffer, pH 7, 1 mM EDTA) for at least 2 hours. The filter was hybridized overnight with probe. Following hybridization, the filters were washed twice with wash solution containing 0.5% BSA, 5% SDS, 40 mM phosphate buffer, pH 7, 1 MM EDTA for 30 min at 65° C. The βAPP cDNA probe corresponded to a unique internal BglII/SpeI fragment generated from human βAPP cDNA (provided by John Kusiak, Gerontology Research Center, IRP, NIA, NIH). Equal loading of samples was verified by rehybridizing the filter with a human actin gene using an actin β-cDNA probe (Clontech Laboratories, Palo Alto, Calif.).
Plasmid Constructs: The plasmid PSV2-APP-CatD was provided by Dr. Rogers (1999). Briefly, the pSV2(APP)CAT construct was generated by inserting a 90 bp fragment of the βAPP gene 5′-UTR immediately upstream of the CAT gene into the pSV2 vector.
Statistics: A two-tailed Student's t-test was carried out to compare two means. When more than two means were compared, one-way analysis of variance, together with a Bartlett's test for homogeneity of variances and a Dunnett's multiple comparison test were used. The level of significance was defined as P<0.05.
Phenserine Decreases βAPP and Aβ Levels in Neuroblastoma Cells
βAPP protein levels were measured after treatment of the SH-SY-5Y cells with 5 μM (+)-phenserine and (−)-phenserine for 0.5, 1, 2, and 4 hours (FIG. 3). SH-SY5Y cells were incubated with 5 μM (+)-phenserine or (−) phenserine for 0, 0.5, 1, 2 and 4 hours to determine the effect of the drug on βAPP protein levels. Western blots of cell lysates containing 15 μg of total protein per lane were analyzed. The blot was sectioned into two halves and the top portion of the blot was probed with an N-terminal directed anti-βAPP antibody whereas the remaining blot was probed with an antibody directed to phosphorylated ERK. In accord with previous reports (Lahiri et al, 1997, 1998; Waskiewics and Cooper, 1995), two high molecular weight bands corresponded to alternate forms of βAPP (100–125 kDa) and ERK 1⁄2 (42–46 kDa). The stereoisomeric forms of the drugs have opposite affects on cholinesterase activity: (+)-phenserine exhibits no anti-cholinesterase activity whereas (−)-phenserine has potent enzymatic activity (Greig et al., 1995; Brossi et al., 1996). In both experiments, the βAPP levels in the cell lysates slowly decreased at each time point with the most dramatic decline observed after 4 hours. During this period, the cells were also examined for their ability to induce signal transduction pathways. Mitogen stimulated kinase, ERK1⁄2, was detected in the treated samples at all times and peaked at the 30 minute to 1 hour period. Stress activated transcription kinases, p38 and JNK, were not detected in the samples. Furthermore, media samples were analyzed for levels of Aβ at 4 hours and, additionally, at 8 and 16 hours to assess whether or not decline in βAPP translated into a decline in total Aβ peptide levels. Levels of Aβ were below detectable levels in both control and (−)-phenserine treated cells. As a consequence, studies were repeated with SK-N-SH cells with (−)-phenserine and (+)-phenserine, which was used in all subsequent studies unless otherwise indicated.
SK-N-SH cells were incubated with either (−)-phenserine (FIG. 4) or with (+)-phenserine (FIG. 5) for up to 16 hours. FIG. 4 illustrates the effect of (−)-phenserine on βAPP protein levels (FIGS. 4A and 4B), LDH levels (FIG. 4C) and total Aβ levels (FIG. 4D). βAPP levels are shown as a percent of controls after pretreatment with 0.5, 5 and 50 μM (−)-phenserine for 4, 8 and 16 hours (*significantly different from control, p<0.05). Western blots of conditioned media (FIG. 4A) and cell lysates (FIG. 4B) were probed with a N-terminal directed anti-βAPP antibody. Following phenserine treatment of SK-N-SH cells for 16 hours, βAPP levels were reduced in a time- and concentration-dependent manner in both conditioned media (FIG. 4A) and cell lysates (FIG. 4B).
LDH levels were measured in media from cells treated with and without 50 μM (−)-phenserine for up to 16 hours. There was no significant difference between treated and untreated levels up to 16 hours p>0.05). This was not associated with cellular dysfunction, as determined by measurement of LDH levels versus untreated controls (FIG. 4C).
Quantification of levels of total Aβ was undertaken at 8 and 16 hours and results shown in FIG. 4D demonstrate a (−)-phenserine induced reduction of 14% and 31% (p<0.002), respectively, versus untreated controls. (+)-Phenserine possessed a similar concentration- and time-dependent action on βAPP levels. The concentration of total Aβ peptide was measured in media from SK-N-SH cells that were incubated with 50 μM (−)-phenserine for up to 16 hours. Levels fell from 6.95 to 5.95 pM (14%) at 8 hours, and from 11.75 to 8.1 pM (31%, p<0.02) at 16 hours in control and (−)-phenserine treated cell respectively (FIG. 4D). Likewise, (+)-phenserine reduced βAPP protein levels and total Aβ peptide levels at 16 hours (FIG. 5). This was not associated with toxicity, as assessed by measurement of cell number and viability (LDH levels).
Phenserine Associated Decrease of βAPP Levels in Astrocytoma Cell Line U373 is not Dependent on ERK Activation
Following an extended period of (−)-phenserine treatment, U373 cells exhibited a similar pattern of decreased βAPP protein synthesis. FIG. 6 is a representative of 4 experiments that showed that βAPP levels gradually decreased between 1 and 8 hours of treatment. U373 MG astrocytoma cells were treated with (−)-phenserine to determine its effect on βAPP protein levels. The addition of ERK inhibitor, PD98059, and PI 3 kinase inhibitor, LY294002, was carried out to ascertain whether or not (−)-phenserine action on βAPP was directed through these signaling pathways. Western blots of lysates (15 μg per lane) of U373 cells incubated with 50 μM of (−)-phenserine for 0, 0.5, 1, 4, 8, 24 and 48 hours were analyzed. The blot was divided into two sections. On the left panel, the blot was probed with anti-βAPP antibody and on the right panel, the blot was probed with anti-phosphorylated ERK antibody. After 8 hours, a slow recovery of βAPP was detectable (FIG. 6A) but its level was still lower than in untreated cells. The activation of ERK1⁄2 peaked at the 30 minute time point and remained elevated at a low level for the remainder of the assay.
In order to determine whether or not ERK involvement was directly related to phenserine treatment, the cells were pretreated with PD98059, a specific inhibitor of MAP kinase (FIG. 6B). U373 MG cells were pretreated with 25 nM PD98059 for 16 hours prior to (−)-phenserine treatment. Lysates were analyzed by western blots as described above. Although ERK levels decreased significantly, the pattern of βAPP levels induced by phenserine remained largely similar to U373 cells treated with drug without PD98059. In all cases, βAPP levels were decreased by in excess of 25%, as determined by densitometric quantification.
Phenserine action on βAPP through ERK independent, phophoinositol 3 kinase (PI 3 kinase) stimulation was also assessed. Treatment of astrocytoma cells with phenserine and LY294002, a specific inhibitor of PI 3 kinase, showed a similar pattern of βAPP levels when compared to (−)-phenserine alone treated cells (FIG. 6C). U373 MG cells were pretreated with 200 μM LY294002 for 1.5 hours prior to the addition of (−)-phenserine. The cell lysate of each sample was analyzed as described above. βAPP protein levels were reduced by in excess of 25% (p<0.05) with (−)-phenserine treatment in FIGS. 6A–C, as determined by densitometric quantification.
In summary, these studies demonstrate that the action of (−)-phenserine to reduce βAPP protein and total Aβ peptide levels did not occur via classical cholinergic or neurotransmitter mediated mechanism, as has been suggested by Buxbaum et al., (1990, 1992, 1994) and Nitsch et al. (1992, 1994). This was supported by two previously unreported lines of evidence. First, studies with the (+)-enantiomer, (+)-phenserine, that is devoid of anticholinesterase activity and hence cholinergic action, still reduced βAPP protein and total Aβ peptide levels (FIG. 5). Second, the actions of (−)-phenserine βAPP protein and total Aβ peptide persisted after the classical pathways involved in cholinergic modulation were blocked (FIG. 6). In addition, in separate studies, when 50 uM concentrations of the classical anticholinesterase, (−)-physostigmine, were applied to SK-N-SH cells, no reduction or change in βAPP protein and total Aβ peptide levels was found.
To demonstrate that the actions on βAPP protein and Aβ peptide were not restricted to enantiomers of phenserine, identical studies were undertaken with both enantiomers of cymserine (compound 46 in Table 1 for (+)-enantiomer) and with (−)-N1,N8-bisnorcymserine and (−)-N1-phenethylcymserine. Similar to (−)-phenserine, the (−)-enantiomer of cymserine possessed anticholinesterase action and the (+)-enantiomer was devoid of it. As shown in FIGS. 7A and 7B, both enantiomers reduce βAPP protein levels. Similarly, N1,N8-bisnorcymserine and N1-phenethylcymserine reduced βAPP protein levels (FIGS. 7C and 7D). In each case, Aβ peptide levels were also reduced, and there was no toxicity (as assessed by cell number and viability, as assessed by LDH measurement). However, at higher concentrations of N1,N8-bisnorcymserine and N1-phenethylcymserine, the compounds are toxic.
Phenserine Decreases βAPP Protein Levels through the Action of a Translational Enhancer in the APP-mRNA 5′ Untranslated Region
A recent report identified a 90 nt element from the 146 nt 5′ untranslated region (5′UTR) of the βAPP mRNA that is able to confer a 3 fold IL-1 responsive gene expression to CAT reporter mRNAs in astrocytoma cells (Rogers et al.,1999). Interleukin-1 was able to induce βAPP protein levels in the absence of increased βAPP mRNA synthesis. Parallel experiments with (−)-phenserine were examined for its ability to regulate βAPP protein levels in an identical manner. U373 MG astrocytoma cells were transfected with 3 μg of pSV2 (APP) CAT plasmid or the parental vector pSV2 CAT. Each set of transfection plates was left unstimulated or treated with 50 μM of phenserine for the experimental times listed below.
FIG. 8A is a representative CAT assay that shows that (−)-phenserine is able to decrease the level of APP-mRNA 5′UTR enhancement to a CAT reporter mRNA in pSV2 (APP) CAT transfected astrocytoma cells. CAT activity was assessed from lysates of transfected cells treated with (−)-phenserine for 0, 0.25, 0.5 and 1 hour and 50 nag from each sample was measured in duplicate for each assay. Quantitation of the fold stimulus of CAT gene activity conferred by the 5′ UTR βAPP-mRNA was measured. ELISA readings of CAT expression were measured at 405 nm A 4-fold decrease after phenserine treatment was sustained after 1 hour. In control samples, pSV2 CAT transfected cells exhibited no inhibition by (−)-phenserine at all time points, indicating that the parental vector was unresponsive to drug treatment In other assays where the time of treatment was extended to 48 hours, only a 2-fold decrease in CAT reporter mRNA was detected with phenserine treatment. However, even in these extended assays, CAT activity in control cells remained undisturbed, indicating that the drug's effects were specific for the 5′ UTR of βAPP mRNA. The expression level of CAT in the control vector vs. the 5′UTR containing vector prior to (−)-phenserine treatment was similar.
(−)-Phenserine decreases the level of βAPP levels through the influence of the βAPP-mRNA 5′UTR region. Western blot analysis of βAPP protein levels was performed on lysates of transfected cells treated with (−)-phenserine for 0, 0.25, 0.5 and 1 hour (FIG. 8B). The blot was probed with anti-βAPP antibody. βAPP protein levels in transfected U373 astrocytoma cells treated with phenserine showed a similar pattern of results as with the CAT assay (FIG. 8A). The introduction of the CAT reporter constructs increased the level of βAPP over the endogenous untransfected cells, in accord with that reported by Rogers et al. (1999). However, after (−)-phenserine treatment, βAPP levels in pSV2 (APP) CAT transfected astrocytomas gradually approached levels seen in untransfected and untreated cells. In contrast to these results, phenserine treatment of U373 cells did not affect βAPP mRNA levels.
FIG. 8C is a representative northern blot of U373 cells treated with phenserine for time points up to 48 hours. Phenserine does not affect the steady state levels of βAPP-mRNA levels. Ten μg of RNA isolated from untransfected and transfected cells treated with (−)-phenserine for 0, 1, 4, 24 and 48 hours were analyzed by northern blot. Phosphoimager analysis revealed steady-state expression of βAPP-mRNA in each sample. The same filter was stripped and rehybridized with a labeled human actin probe to standardize the loading differences in individual samples. Standardization of each sample to actin mRNA expression showed consistent levels of βAPP mRNA without any major fluctuations in densitometry readings. Clearly, phenserine's action of βAPP protein is at the level of translation, as northern blot analysis of untransfected and transfected cells show little differences in levels of mRNA transcription.
In Vivo Studies—Toxicity of (−)-Phenserine vs. (+)-Phenserine
On administration of (−)-phenserine to rodents by the i.p. route (1 ml/kg in 0.9% saline) a fine tremor is observed at a dose of 5 mg/kg. This persisted for an hour and is related to central cholinergic overdrive. Tremor together with symptoms of peripheral cholinergic overdrive (specifically, salivation and lacrimation) were seen at a dose of 7.5 mg/kg for some 3 hours. Animals were incapacitated at 20 mg/kg (N=3 per dose group), and 2 were killed when moribund. Animals (N=2) administered 20 mg/kg (+)-phenserine were without clinical symptoms and appeared normal.
Illustrated in FIG. 9 is the action of (−)-phenserine (2.5 mg/kg i.p., once daily for 3 weeks) on brain cortex and CSF βAPP levels in transgenic mice (N=12) that significantly overexpress βAPP as a consequence of the human Swedish βAPP mutation and mutant presenilin 1 (Borchelt et al., 1997). (−)-Phenserine significantly reduced βAPP levels by 55% in CSF and 10% in cerebral cortex. Greater reductions could be achieved, but, as previously reported, doses of >5 mg/kg produce cholinergic side effects for the (−)-enantiomer but not for the (+)-enantiomer.
Samples of cerebral cortex from the transgenic mice then were analyzed for β-amyloid peptide (Aβ) levels. Specifically, Aβ1-40 and Aβ1-42 levels, following formic acid extraction, were determined by ELISA assay (Suzuki et al., 1994). As shown in FIG. 10A, (−)-phenserine treatment reduced Aβ1-40 levels by 68% (p<0.05), whereas (−)-N1-phenethylcymserine reduced levels by 54% (p<0.05). In contrast, (−)-phenserine reduced Aβ1-42 levels by 53% (p<0.05), compared to a 47% reduction (p<0.05) induced by (−)-N1-phenethylcymserine, as shown in FIG. 10B.
Hence, even over as short a duration as three weeks in the life span of transgenic mice (generally 18 to 24 months) that overexpress βAPP and overproduce Aβ, daily phenserine and N1-phenethylcymserine administration, in well tolerated doses, reduce both βAPP and Aβ levels, thereby indicating that in vitro efficacy translates to in vivo activity.
In summary, the (+)-enantiomers of this invention are the focus of the present application. They are unnatural and totally synthetic compounds. The described studies demonstrated that both (+)- and (−)-enantiomers possessed potent activity to reduce βAPP protein and total Aβ peptide levels. However, the (+)-enantiomers are devoid of anticholinesterase activity, and hence lack cholinergic action. It is the cholinergic action that is dose limiting with regard to the use of the (−)-enantiomers in vivo. The reductions in levels of βAPP protein demonstrated in tissue culture studies occurred in two different types of human neuroblastoma cell (SK-N-SH and SH-SY-5Y lines), as well as in astrocytoma cells (U 373 line). These in vitro effects translate into in vivo activity as demonstrated herein.
Translational Effect of Phenserine on APP
1. Rate of APP Synthesis
8×106 SHSY-5Y cells were plated on 100 mm dishes with DMEM containing 10% FBS. After 36 hours, the culture medium was replaced with DMEM containing 0.5% FBS. The cells were incubated with low serum medium for 1 hour. Thereafter, the medium was replaced with fresh low serum medium with and without 10 μM of phenserine for 16 hours.
After treatment (16 hrs) with and without (−)-phenserine (10 μM), the cells were incubated with methionine and cystine free DMEM containing 4 mM of glutamine for 1 hour. After treatment with methionine and cystine free medium, 2 ml of 35S-labeled DMEM (100 μMCi/ml) with and without phenserine (10 μM) were added and incubated for 10 minutes. Thereafter, the labeled medium was carefully removed and the cells were suspended in lysis buffer containing with protease inhibitors and frozen at −80 C for use in the immunoprecipitation assay.
APP protein was immunoprecipitated from 300 μg of total protein in each sample using the polyclonal antibody O443, which recognizes a 20 amino acid sequence in the carboxy terminal of APP, and protein A/G resin overnight at 4° C. Immunoprecipitated APP was eluted from the protein A/G resin with 30 μl of elution buffer (10% beta-mercaptoethanol). The samples were loaded onto 10% trys-glycine gels, and the proteins were separated at 150 V for 90 min. The gels were fixed and dried at 80 C for 60 min. The dried gels were exposed to Phosphor Screen (PACKARD Instrument Company, Inc., Meriden, Conn.) overnight and the βAPP signals were quantitated on a phosphor imager. Phenserine significantly decreased βAPP synthesis (52% reduction) without changing TCA precipitable counts (FIG. 11A). This change in APP protein was not reflected in a change in mRNA levels as phenserine did not alter levels of APP RNA (FIG. 11B).
2. Effect of Phenserine on Steady State APP Levels
3×106 SK-N-SH cells were plated on 60 mm dishes with DMBM containing 10% FBS. After 36 hours, cells were incubated with low serum medium for 1 hour. Thereafter, the medium was replaced with fresh low serum medium (0.5% FBS). To start the experiments, spent medium was removed and replaced with fresh medium containing 0, 0.5, 5 or 50 μM of (+)- or (−)-phenserine. The cells were incubated with and without phenserine for 16 hours. At the stated time (4, 8 and 16 hrs), 200 μl of medium was transferred from each dish for assessment of extracellular APP levels. At the end of experiments (16 hrs), the cells were lysed and collected for assessment of intracellular APP levels.
15 μl of medium samples and 15 μg of total protein from each lysed samples were loaded onto 10% trys-glycine gels, and the protein was separated at 150 V for 90 min. The gels were transferred onto polyvinylidene difuoride paper and probed with an affinity-purified anti-APP antibody (22C11), which recognizes the ectodomain of APP (residues 66–81). The APP signals were detected by chemiluminescence and exposed to film. The quantitation of the signals was determined by using a CD camera and NIH-IMAGE (version 4.0).
Phenserine significantly decreased steady state levels of extra- and intracellular βAPP in dose and time dependent manner (FIGS. 12A and B). Under these conditions, there was no significant toxicity as assessed by an LDH assay.
Screening for Inhibitors of APP
1. Synthesis of Compounds of Carbamate Non-Carbamate Compounds
N,N-Dimethyl-N1-benzyl-5-methoxytryptamine (MES 9191)




N,N-Dimethyl-5-methoxytryptamine (654 mg, 3.0 mmol) and NaNH2 (234 mg, 60 mmol) were added into THF (10 ml), then benzyl bromide (513 mg, 3.0 mmol) was added. The mixture was refluxed under nitrogen with stirring for 2 days. Workup gave MES 9191 281 mg (30%).
(−)-(5aS)-3,5a,10-Trimethyl-1,3,4,5,5a,10a,10-heptahydro-1,3-diaza-2-one-7-hydroxycyclohept[2,3-b]indole (MES 9205)




Method 1:15 Eseroline (180 mg, 0.82 mmol) and trimethylsilyl isocyanate (48 mg, 0.42 mmol) were dissolved in toluene (2 ml) in a sealed tube. The reaction mixture was stirred by a small magnetic bar and heated in an oil bath for 6 hours. The temperature of the oil was maintained between 100 and 110° C. After cooling to room temperature, the precipitated crystals were filtered and recrystallized from MeOH to give carbamate MES 9205 (64 mg, 30%): m.p. 174–176° C.;[a]D20=−154° (c=0.5, EtOH); CI-MS(NH3) m/z, 262 (MH+); 1HNMR (CD3OD): 6.59 (d, J=3.0 Hz, 1H, C6-H), 6.53 (m, 1H, C8-H), 6.43 (d, J=8.0 Hz, 1H, C9-H), 3.88 (s, 1H, C10a-H), 3.20 (m, 1H, C4-H′), 2.95 (m, 1H, C4-H′′), 2.84 (s, 3H, N10-CH3), 2.62 (s, 3H, N3-CH3) 2.19 (m, 1H, C5-H′), 1.81 (m,1H, C5-H′′), 1.30 (s, 3H, C5a-CH3). Anal (C14H19N3O2) C, H, N.
3-Methoxy-(1′-N-methylamino)-ethyl-benzene (MES 9271)




3-Methoxyacetophone (2 g, 0.013 mol) with methylamine (6 g,) was refluxed in ethanol for 4 h. After evaporation of solvent chromatography gave schiff base (1.7 g, 80%). The schiff base of 3-methoxyacetophone (1.25 g, 0.008 mol) was dissolved in methanol (25 ml) and reduced by sodiumborohydride (0.24 g, 0.008 mol). Workup gave MES 9271 1 g (80%).
Propionamide (MES 9291).







N-Methylphenetidine (340 g, 1.85 mol) was dissolved into 750 ml of benzene and cooled to 10° C., then α-bromopropionylbromide (200 g, 0.926 mol) was rapidly added. The mixture was stirred for 1.5 h at 40° C. then washed by water and 1.5%. HCl. Evaporation gave product MES 9291 477 g (100%).
1,3-Dimethyl-5-hydroxyoxindole (MES 9292). The propionanilde (MES 9291) (477 g, 1.85 mol) was mixed with 450 g AICl3 then heated by an oil bath to 190° C. for 1 h. After reaction the mixture was poured into ice water, the precipitate was filtered and crystallized to give product MES 9292 148 g (91%).
(+)-O-Methyleseroline (MES 9295). The 1,3-dimethyl-5-hydroxyindole (MES 9292) was chiral alkylated by chloroacetonitrile in using the chiral catalyst CPTC to afford optical rich product, (3R)-3-cyanomethyl-5-methoxyoxindole, which then purified by chiral chromatography to give optical pure (3R)-3-cyanomethyl-5-methoxyoxindole. Reductive cyclization of above cyano-oxindole by reducing agent red-Al gave (+)-N1-O-methylnoreseroline which then was methylated by formaldehyde and sodiumborohydride to give MES 9295 in the yields as described in the scheme.
(−)-O-Tetrahydropyranyl-N1-noreseroline (MES 9320)




Starting material Nitrile (120 mg, 0.4 mmol) and virtride (0.15 ml, 0.4 mmol) were dissolved in toluene. The mixture was stirred under nitrogen at room temperature for 3 h, then 5 ml of 5% NaOH was added. The toluene layer was separated out and the aqueous layer was extracted with ether (2×5 ml). The combined organic layers were washed with brine, dried over sodium sulfate, evaporated in vacuum to give MES 9320 119 mg (92.5%).
1,3-Dimethyl-3-hydroxy-5-methoxyoxindole (MES 9323)




To a 25 ml flask was added benzene (12 ml) and oxindole (95.5 ml, 0.5 mmol, then 2 ml of 50% NaOH solution were added. The mixture after standing for 12 h was extracted with ether (3×10 ml). The combined extracts were washed with brine, and dried over MgSO4. After evaporation of solvent and chromatography (silica gel, petroleum ether: EtOH=3:1) gave MES 9323 62 mg (50%).
2. ELISA Assay for Identification of APP Inhibitors
An enzyme-linked immunosorbent assay was developed to detect secreted APP in SHSY5Y cells, a human neuroblastoma cell line. The purpose of the screen was to discover small molecules that inhibit APP protein synthesis in SHSY5Y cells. SHSY5Y cells were plated at 1×105 cells/well in 100 mL/well of DMEM containing 0.5% heat inactivated FBS in 96 well tissue culture treated plates (Falcon no.35 3072). 144 MES compounds were tested at final concentrations of 20 mM, 6.7 mM, and 2.2 mM in 0.1% DMSO. Conmpounds were added and the plates incubated for 16 hours at 37° C./5% CO2. Maxisorp plates (Nunc no. 437958) were coated overnight with 2 mg/mL capture antibody (Biosource 44–100) diluted in Ca++ and Mg++ free PBS. Biosource 44–100 is a mouse mAb that recognizes aa 1–100 of human APP. Plates were blocked for 30 minutes with 1 mg/mL BSA in Ca++ and Mg++ free PBS. Plates were washed 3× with wash buffer (Ca++ and Mg++ free PBS+0.01% Tween20). 50 mL of supernatants and non-cultured medium controls were transferred from culture plates to ELISA plates. Culture plates were reserved for toxicity analysis. Supernatants were incubated for 4 hours at RT on a plate shaker. After supernatant incubation, plates were washed 3×. Primary antibody (Signet Clone 6E-10 biotin) was added at 0.3125 mg/mL and incubated overnight at 4° C. The recognition site of 6E-10 is aa 1–17 of Ab. For detection, a 1:3000 dilution of horseradish peroxidase conjugated streptavidin (Endogen no. N-100) was incubated for 30 minutes. Plates were washed 3×. Enzyme activity was assessed by incubation with 100 mL/well TMB substrate solution (Moss no. TMB-US) for 20 minutes. The reaction was stopped by the addition of 100 mL/well 0.18M sulfuric acid. Optical density was read at 450 nm on Wallac Victor2 plate reader. During the first incubation, toxicity was determined using MTS assay, Cell Titer96 AQ reagent (Promega no. G5430). The background values from the non-cultured medium control were subtracted from the sample values and secreted APP levels and toxicity were expressed as % vehicle control. Of the 144 MES compounds screened, 12 inhibited secreted APP as determined by ELISA. The results are depicted in FIGS. 13A–M.
Using the experimental procedure outlined above, APP secretion and toxicity studies were performed on several other non-carbamate compounds, and the results are shown in Tables 3 and 4. The compound structures are provided in Table 2 in the application, which are identified by their MES number.









TABLE 3









APP Secretion by SH-SY-5Y Cells


















Dose (uM)
Dose (uM)
Dose (uM)
Dose (uM)
Dose (uM)
Dose (uM)


Number
NIH #
MES
0.08
0.25
0.74
2
6
20


















6
594xp2
9191
100.7666667
101.6333333
99.86666667
100.74
65.3
64.36


15
y-1-15
9199



92.2
93.9
102.95


17
y-1-46
9201



97.25
110.75
124.65


18
y-1-48-1 (−)
9202



94.4
107.55
110.05


19
y-1-48-2 (+)
9203



103.1
103.866667
108.36667


21
y-1-59
9205
115.1
107.4
114.7
96.7333333
96.9
22.166667


22
y-1-60
9206



88.2
97.1
96.4


27
y-1-66
9215



94.55
108.1
6.2


34
y-2-1
9222



99.85
105.55
120.4


37
y-2-13
9225



110.45
109.2
59.75


38
y-2-15
9226



116.55
101.95
104.65


39
y-2-16
9227



101.15
99.45
120.5


40
y-2-18
9228



97.75
110.45
130.05


41
y-2-19
9229



111.6
111.4
125.1


42
y-2-20
9230



101.25
88.8
4.4


43
y-2-24
9231



104.9
133.5
154.2


46
y-2-31
9234



106.4
96.5
98.8


48
y-2-33
9236



105.8
103.2
101.65


49
y-2-34
9237



149.95
158.1
173.3


50
y-2-35
9238
117.6
107.7
109.7
48.5666667
39.2333333
23.566667


54
y-2-43-2
9242



116.75
116.3
116.1


55
y-2-49
9243



88.95
95.25
100.55


69
y-2-95
9257



111.2
98.9
99.033333


71
y-2-105
9259



90.7
101.1
111.1


72
y-2-106
9260



93.95
103.2
101.1


78
y-2-113
9266



94.4
95.2
94.85


79
y-2-114
9267
97.8
97.43333333
97.43333333
93.68
68.64
22.22


82
y-2-130
9270



95.8
95.9
98.15


83
y-2-131
9271
96.6
87.95
92.2
96.1
89.9
63.825


87
y-3-5
9276



96.15
116.95
133.4


89
y-3-15
9277



98.25
109
113.6


90
y-3-17
9278



118.4
116.766667
120.63333


91
y-3-19
9279
97.8
98.4
97
43.2666667
42.3666667
18.4


104
y-3-58
9291
98.2
99.5
99.7
100.133333
96.9
67.8


105
y-3-59
9292



106.15
99.55
100.15


106
y-3-60
9293



97.8
102.75
94.45


107
y-3-69
9294



105.25
90
111


108
y-3-71
9295



102.6
95.7
89.35


114
SH-281
9301



108.45
106.1
111.15


115
140xp2
9302



100.35
105.4
105.8


118
614xp1
9305



106.2
102.4
102.2


119
552xp3
9306



110.75
114.55
124.8


122
piy-1-79-2
9309



100
106.4
100.9


123
129xp3
9310



104.8
97.85
98.95


124
637xp4
9311



102.45
103.05
102.7


125
piy-1-79-3
9312



105.4
101.7
96.65


127
piy-1-79-4
9314



111.25
99.15
109.4


129
piy-1-79-5
9316



104.15
98.45
102


130
597xp4
9317



99.8666667
112.266667
122.03333


131
197xF3
9318



104.05
103.4
108.75


132
504xp2
9319



108.95
114.1
112.8


133
338xp2
9320



103.15
91.8
15.2


134
574xp1
9321



97.95
98.1
100.5


136
111xp1
9323



98.4
102.3
98.3


137
484xp2
9324



110.3
95.9
99.95


143
y-1-78-1
9330



99.6
101.5
110.65


144
y-1-78-2
9331



95.45
102.1
106.7


















TABLE 4









APP Toxicity SH-SY-5Y Cells


















Dose (uM)
Dose (uM)
Dose (uM)
Dose (uM)
Dose (uM)
Dose (uM)


Number
NIH #
MES
0.08
0.25
0.74
2
6
20


















6
594xp2
9191
104.4666667
103.9
103.7
99.18
92.84
91.1


15
y-1-15
9199



100.5
101.65
101.9


17
y-1-46
9201



98.9
98.65
98.25


18
y-1-48-1 (−)
9202



99.15
97.85
90.25


19
y-1-48-2 (+)
9203



98.93333333
99.16666667
98.83333333


21
y-1-59
9205
108.7
105.8
105.6
97.9
97.73333333
33.33333333


22
y-1-60
9206



96
91.85
76.3


27
y-1-66
9215



92.3
78.6
12.9


34
y-2-1
9222



99.25
100.5
98.9


37
y-2-13
9225



104.6333333
100.1
66.5


38
y-2-15
9226



106.3666667
99.7
97.9


39
y-2-16
9227



103.6666667
99.26666667
96.06666667


40
y-2-18
9228



102.7666667
97.6
95.4


41
y-2-19
9229



99.96666667
96.06666667
91.46666667


42
y-2-20
9230



100.8333333
86.13333333
13.83333333


43
y-2-24
9231



95.7
90.73333333
85.86666667


46
y-2-31
9234



102.55
99.75
99.3


48
y-2-33
9236



97.6
101
100.05


49
y-2-34
9237



96.55
98.35
99.4


50
y-2-35
9238
101.8
103.8
106.4
41.8
40.46666667
31.63333333


54
y-2-43-2
9242



94.2
89.8
80.95


55
y-2-49
9243



99.1
98.4
99


69
y-2-95
9257



100.5333333
98.76666667
100


71
y-2-105
9259



102.65
102.9
98.75


72
y-2-106
9260



99.8
97.2
98.95


78
y-2-113
9266



96.55
97.75
94.15


79
y-2-114
9267
101.5333333
102
101.9333333
97.98
96.72
15.52


82
y-2-130
9270



99.4
101.1
100.4


83
y-2-131
9271
103.55
103.45
103.1
99
99.475
94.1


87
y-3-5
9276



92.85
93.5
90.65


89
y-3-15
9277



98.25
98.3
91.1


90
y-3-17
9278



97.63333333
98.4
96.33333333


91
y-3-19
9279
102.9
101.1
100.1
68.63333333
46.66666667
38.63333333


104
y-3-58
9291
99.1
100.5
97.1
102.3666667
99.86666667
99


105
y-3-59
9292



102.85
103.75
104.9


106
y-3-60
9293



100.8
99.85
101.95


107
y-3-69
9294



99.9
98.3
100.2


108
y-3-71
9295



101.15
100.4
99.85


114
SH-281
9301



99.15
97.35
95.9


115
140xp2
9302



100.7
101.2
99.05


118
614xp1
9305



95.8
96.73333333
94.83333333


119
552xp3
9306



102.3
101.45
106


122
piy-1-79-2
9309



100.85
98.6
101.1


123
129xp3
9310



101.85
99.9
97.7


124
637xp4
9311



101.1
101.25
99.55


125
piy-1-79-3
9312



99.95
103.85
106.95


127
piy-1-79-4
9314



100.15
100.85
100.6


129
piy-1-79-5
9316



99.15
98.05
98.75


130
597xp4
9317



97.26666667
97.23333333
93.5


131
197xF3
9318



96.2
96.45
96.05


132
504xp2
9319



99.8
101.05
102.7


133
338xp2
9320



92
84
28


134
574xp1
9321



99.9
101.15
99.45


136
111xp1
9323



97.2
100.1
101.15


137
484xp2
9324



102.15
100.4
98.65


143
y-1-78-1
9330



99.05
98.45
97.95


144
y-1-78-2
9331



100.45
99.9
98.95









2. Effect of Phenserine Analogues on Steady State APP Levels

3×106 SK-N-SH cells were plated on 60 mm dishes with DMEM containing 10% FBS. After 36 hours, cells were incubated with low serum medium for 1 hour. Thereafter, the medium was replaced with fresh low serum medium (0.5% FBS). To start the experiments, spent medium was removed and replaced with fresh medium with and without phenserine analogues. The cells were incubated with and without compounds for 16 hours. At the end of experiments (16 hrs), 200 ml of medium and the cells were lysed and collected for assessment of intracellular APP levels.
20 ml of medium samples and 15 mg of total protein from each lysed samples were loaded onto 10% trys-glycine gels, and the protein was separated at 150 V for 90 min. The gels were transferred onto polyvinylidene difuoride paper and probed with an affinity-purified anti-APP antibody (22C11), which recognizes the ectodomain of APP (residues 66–81). The APP signals were detected by chemiluminescence. The quantitation of blots was undertaken by using a CD camera and NIH-IMAGE (version 4.0).
Several of the compounds of the invention decreased the extra- and intracellular APP levels. A few compounds, which have no carbamate group within the molecule, did not show significant reductions of intracellular APP levels, but did reduce extracellular APP levels. The treatment with 5 mM of MES9280 showed some toxicity (increase of LDH levels and morphological change). The results are shown in FIG. 14.
3. Effect of Phenserine Analogues on APP Translation
3×106 SHSY-5Y cells were plated on 60 mm dishes with DMEM containing 10% FBS. After 36 hours, the culture medium was replaced with DMEM containing 0.5% FBS. The cells were incubated with low serum medium for 1 hour, thereafter, the medium was replaced with fresh low serum medium with and without phenserine analogues for 16 hours.
After treatments (16 hrs) with and without the compounds of the invention, the cells were incubated with methionine and cystine free DMEM containing 4 mM of glutamine for 1 hr. After treatment with methionine and cyctine free medium, 1 ml of 35S-labeled DMEM (100 uCi/ml) with and without the compounds were added and incubated for 10 minutes. Thereafter, the labeled medium were carefully removed and the cells were suspended within lysis buffer containing with protease inhibitors and frozen at −80° C. until immunoprecipitation assay.
APP were immunoprecipitated from 200 ug of total protein in each samples with polyclonal antibody O443, which recognizes 20 aminoacids sequences of APP carboxy terminal, and protein A/G resin for overnight at 4 C. Immunoprecipitated APP were eluted from protein A/G resin with 30 ul of elution buffer (10% beta-mercaptoethanol). The samples were loaded onto 10% trys-glycinegels, and the protein were separated at 150 V for 90 min. The gels were fixed with fixing buffer and dried at 80 C for 60 min. The dried gels were exposed onto Phosphor Screen (PACKARD, Instrument Company, Inc., Meriden, Conn.) for overnight and the APP signals were quantitated on phosphor image.
The levels of newly synthesized βAPP were normalized by TCA precipitable counts. Several phenserine analogues significantly decreased βAPP synthesis without changing TCA precipitable counts (FIG. 15A). In addition, there was no change in APP mRNA levels (FIG. 15B).
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REFERENCES FOR FIG. 1


    1. Qian-sheng Yu, Arnold Brossi. Practical Synthesis of Unnatural (+)-Physostigmine and Carbamate Analogues. Heterocycles, 1988, 27, 745–750.
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    3. Qian-sheng Yu, Xue-Feng Pei, Harold W. Holloway, Nigel H. Greig, Arnold Brossi. Total Synthesis and Anticholinsterase Activities of (3aS)-N(8)-Norphysostigmine, (3aS)-N(8)-Norphenserine, Their Antipodal Isomers, and Other N(8)-Substituted Analogues. J. Med. Chem., 1997, 40, 2895–2901.
    4. Qian-sheng Yu, Nigel H. Greig, Harold W. Holloway, Arnold Brossi. Syntheses and Anticholinesterase Activities of (3aS)-N1,N8-Bisnorphenserine, (3aS)-N1,N8-Bisnorphysostigmine, Their Antipodal Isomers and Other Potential Metabolites of Phenserine. J. Med. Chem., 1998, 41, 2371–2379.
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REFERENCES FOR FIG. 2


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## Claims

1. A compound comprising formula I



wherein R1 and R2 are, independently, hydrogen, branched or straight chain C1–C8 alkyl, substituted or unsubstituted aryl, or aralkyl;
R3 is branched or straight chain C1–C4 alkyl or heteroalkyl or C4–C8 alkyl or heteroakyl, or substituted or unsubstituted aryl;
X and Y are, independently, O, S, alkyl, hydrocarbon moiety, C(H)R4, or NR5, wherein R4 and R5 are, independently, hydrogen, oxygen, branched or straight chain C1–C8 alkyl, C2–C8 alkenyl, or C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl;
wherein the compound is the substantially pure (+)-enantiomer,
wherein the proviso that the compound is not (+)-physostigmine,
(+)-octylcarbamoyleseroline, (+)-benzylcarbamoyleseroline, (+)-physovenine, (+)-N-methylphysostigmine, (+)-phenserine, (+)-3-(1-methylamino)-ethyl-2H-indol-5yl-phenylcarbamate, (+)-N1-benzylnorphysostigmine, (+)-N1-benzylnorphenserine, (+)-N1-benzylnortolserine, (+)-N1-benzylnorcymserine, (+)-N1-norphysostigmine, (+)-N1-norphenserine, (+)-N1-nortolserine, (+)-N1-norcymserine, (+)-N8-benzylnorphysostigmine, (+)-N8-benzylnorphenserine, (+)-N8-norphysostigmine, (+)-N8-norphenserine, (+)-N1, N8-bisbenzylnorphysostigmine, (+)-N1, N8-bisbenzylnorphenserine, (+)-N1,N8-bisnorphysostigmine, or (+)-N1,N8-bisnorphenserine,
with the proviso that when the compound is formula I, X is NCH3, and Y is NR5, where R5 is hydrogen, loweralkyl, arylloweralkyl, heteroarylloweralkyl, cycloalkylmethyl, or loweralkenyl methyl, R3 is not methyl or at least one of R1 or R2 is not H or alkyl.

2. The compound of claim 1, wherein R3 is methyl and X is NCH3 or NCH2Ph.

3. The compound of claim 1, wherein R3 is methyl, X is NCH3, and Y is NCH3.

4. The compound of claim 3, wherein R1 is C1–C8 straight chain alkyl or benzyl and R2 is hydrogen.

5. The compound of claim 3, wherein R1 is substituted or unsubstituted phenyl and R2 is hydrogen.

6. The compound of claim 3, wherein R1 and R2 are, independently, methyl or ethyl.

7. The compound of claim 1, wherein R3 is methyl, X is NCH3, and Y is O.

8. The compound of claim 7, wherein R1 is C1–C8 straight chain alkyl or benzyl and R2 is hydrogen.

9. The compound of claim 7, wherein R1 and R2 are, independently, methyl or ethyl.

10. The compound of claim 7, wherein R1 is substituted or unsubstituted phenyl and R2 is hydrogen.

11. The compound of claim 1, wherein R3 is methyl, X is NCH3, and Y is S.

12. The compound of claim 11, wherein R1 is C1–C8 straight chain alkyl or benzyl and R2 is hydrogen.

13. The compound of claim 11, wherein R1 and R2 are, independently, methyl or ethyl.

14. The compound of claim 11, wherein R1 is substituted or unsubstituted phenyl and R2 is hydrogen.

15. The compound of claim 1, wherein R3 is methyl, X is NCH3, and Y is NR5.

16. The compound of claim 15, wherein R5is —CH2CH═CH2, —CH2CH2Ph, benzyl, or hydrogen.

17. The compound of claim 1, wherein R3 is methyl, Y is NCH3, and X is NCH3.

18. The compound of claim 1, wherein Y is C(H)R4, R3 is methyl, and X is NCH3, wherein R4 and R5 are, independently, hydrogen or benzyl.

19. The compound of claim 1, wherein R3 is phenyl and Y is NR5, where R5 is methyl or aralkyl.

20. The compound of claim 1, wherein when R3 is methyl and X is NCH3, Y is not NH or NHCH2Ph.

21. The compound of claim 1, wherein the compound comprises (+)-cymserine, (+)-N1-phenethylnorcymserine, or (+)-N1,N8-bisnorcymserine.

22. The compound of claim 1, wherein X is NR5, wherein R5 is aralkyl, and Y is NCH3.

23. The compound of claim 1, wherein Y is NR5, wherein R5 is aralkyl, and X is NCH3.

24. The compound of claim 1, wherein Y is NR5, wherein R5 is aralkyl, and X is NH.

25. The compound of claim 1, wherein X and Y are NR5, wherein R5 is aralkyl.

26. The compound of claim 1, wherein R1 is aryl, Y is NR5, wherein R5 is aralkyl, and X is NCH3.

27. The compound of claim 1, wherein R1 is aryl, Y is NR5, wherein R5 is aralkyl.

28. The compound of claim 1, wherein R1 is aryl, X is NR5, wherein R5 is aralkyl, and Y is NCH3.

29. The compound of claim 1, wherein R1 is aryl, X and Y are NR5, wherein R5 is aralkyl.

30. The compound of claim 1, wherein R1 is hydrogen, R2 is aryl wherein R2 is not phenyl, R3 is methyl, and X and Y are NR5, wherein R5 is benzyl.

31. A compound comprising the formula II



wherein R1 and R2 are, independently, hydrogen, branched or straight chain C1–C8 alkyl, substituted or unsubstituted aryl, or aralkyl;
R3 is branched or straight chain C1–C4 alkyl or heteroalkyl or C4–C8 alkyl or heteroakyl, or substituted or unsubstituted aryl;
X is O, S, alkyl, hydrocarbon moiety, C(H)R4, or NR5, wherein R4 and R5 are, independently, hydrogen, oxygen, branched or straight chain C1–C8 alkyl, C2–C8 alkenyl, or C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl; and wherein,
R6 is hydrogen; C1–C8 alkyl, C2–C8 alkenyl, C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl, or (CH2)nR7, where R7 is hydroxy, alkoxy, cyano, ester, carboxylic acid, substituted or unsubstituted amino, and n is from 1 to 4, wherein the compound having the formula II is the substantially pure (−)-enantiomer, the substantially pure (+)-enantiomer, or a racemic mixture of the (−)-enantiomer and (+)-enantiomers.

32. The compound of claim 31, wherein R3 is methyl, X is NCH3, R6 is (CH2)2R7, where R7 is a substituted or unsubstituted amino group, and the compound is the substantially pure (+)-enantiomer.

33. A compound comprising formula VII,



wherein R5 is aralkyl and R8 is branched or straight chain C1–C8 alkyl, substituted or unsubstituted aryl, or aralkyl.

34. The compound of claim 33, wherein R5 is benzyl and R8 is methyl.

35. A compound comprising formula V or VI.



wherein R8 is hydrogen, branched or straight chain C1–C8 alkyl, substituted or unsubstituted aryl, aralkyl, or CR9R10OR11, where R9 and R10 are, independently, hydrogen or alkyl, and R11 is alkyl;
R3 is branched or straight chain C1–C4 alkyl, or substituted or unsubstituted aryl;
X and Y are, independently, O, S, C(H)R4, or NR5, wherein R4 and R5 are, independently, hydrogen, oxygen, branched or straight chain C1–C8 alkyl, C2–C8 alkenyl, or C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl; and
R6 is hydrogen; C1–C8 alkyl, C2–C8 alkenyl, C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl, or (CH2)nR7, where R7 is hydroxy, alkoxy, cyano, ester, carboxylic acid, substituted or unsubstituted amino, and n is from 1 to 4,
with the proviso that when the compound is formula V, X is NCH3, and Y is NR5, where R5 is hydrogen, loweralkyl, arylloweralkyl, heteroarylloweralkyl, cycloalkylmethyl, or loweralkenyl methyl, R3 is not methyl or R8 is not H or loweralkyl.

36. The compound of claim 35, wherein the compound is the substantially pure (+)-enantiomer.

37. The compound of claim 35, wherein the compound is the substantially pure (−)-enantiomer.

38. The compound of claim 35, wherein when the compound i0s formula V, then X and Y are NR5.

39. The compound of claim 38, wherein R5 is branched or straight chain C1–C8 alkyl.

40. The compound of claim 38, wherein R5 is methyl.

41. The compound of claim 35, wherein the compound is formula V, R3 is methyl and X is NCH3.

42. The compound of claim 35, wherein the compound is formula V, R3 is methyl, X is NCH3, and Y is NCH3.

43. The compound of claim 35, wherein the compound is formula V, and R8 is C1–C8 straight chain alkyl.

44. The compound of claim 35, wherein the compound is formula V, and R8 is methyl.

45. A compound comprising formula X



wherein R1 and R2 are, independently, hydrogen, branched or straight chain C1–C8 alkyl, substituted or unsubstituted aryl, heteroaryl, or aralkyl;
R3 is branched or straight chain C1–C4 alkyl or heteroalkyl or C4–C8 alkyl or heteroalkyl, or substituted or unsubstituted aryl;
X is NR5, wherein R5 is C2-8 alkenyl, C2-8 alkynyl, or aralkyl, and
Y is selected from C(H)R4 or NR5, wherein R4 and R5 are, independently, hydrogen, branched or straight chain C1-8 alkyl or heteroalkyl, alkenyl, or C2–C8 alkynyl, aralkyl;
wherein the compound is the substantially pure (−)-enantiomer.

46. The compound of claim 45, wherein X is NR5,-wherein R5 is aralkyl.

47. The compound of claim 45, wherein X and Y are NR5, wherein R5 is aralkyl.

48. The compound of claim 45, wherein X is NR5, where R5 is aralkyl, and Y is NR5, wherein R5 is branched or straight chain C1-8 alkyl or heteroalkyl.

49. The compound of claim 45, wherein R1 is branched or straight chain C1–C8 alkyl, aralkyl or aryl, R2 is hydrogen, branched or straight chain C1–C8 alkyl, substituted or unsubstituted aryl, or aralkyl; Y is NR5, wherein R5 is aralkyl; and X is NR5, wherein R5 is hydrogen, branched or straight chain C1-8 alkyl or heteroalkyl.

50. The compound of claim 45, wherein R1 is branched or straight chain C1–C8 alkyl, aralkyl or aryl; R2 is hydrogen, branched or straight chain C1–C8 alkyl; Y is NR5 where R5 is benzyl; and X is NR5, wherein R5 is hydrogen, branched or straight chain C1-8 alkyl or heteroalkyl.

51. The compound of claim 45, wherein R1 is branched or straight chain C1–C8 alkyl, aralkyl or aryl; R2 is hydrogen, branched or straight chain C1–C8 alkyl; Y is NR5 where R5 is benzyl; and X is NR5, wherein R5 is hydrogen.

52. The compound of claim 45, wherein R1 is para-halophenyl; Y is NCH3; and X is NR5, wherein R5 is alkyl or aralkyl, wherein R1 is not para-phenyl bromophenyl when R5 is benzyl.

53. The compound of claim 45, wherein R1 is para-isopropyl phenyl; R2 is hydrogen; R3 is methyl; Y is NR5 where R5 is benzyl; and X is NR5, wherein R5 is hydrogen.

54. A method of inhibiting production of amyloid precursor protein in a mammalian cell, comprising contacting the mammalian cell with a compound comprising formula I



wherein R1 and R2 are, independently, hydrogen, branched or straight chain C1–C8 alkyl, substituted or unsubstituted aryl, or aralkyl;
R3 is branched or straight chain C1–C4 alkyl or heteroalkyl or C4–C8 alkyl or heteroakyl, or substituted or unsubstituted aryl;
X and Y are, independently, O, S, alkyl, hydrocarbon moiety, C(H)R4, or NR5, wherein R4 and R5 are, independently, hydrogen, oxygen, branched or straight chain C1–C8 alkyl, C2–C8 alkenyl, or C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl;
wherein the compound is the substantially pure (+)-enantiomer,
wherein the compound inhibits production of amyloid precursor protein in the mammalian cell.

55. A method of inhibiting production of amyloid precursor protein in a mammalian cell, comprising contacting the mammalian cell with a compound comprising (+)-phenserine, (+)-cymserine, (+)-N1-phenethylnorcymserine, (+)-N1, N8-bisnorcymserine, (+)-N1, N8-bisbenzylnorphenserine, (−)-N8-benzylnorphenserine, (3aS,8aS)-1-benzyl-3a,8-dimethyl-1,2,3,3a,8,8a-hexahydropyrrolo[2,3-b]indol-5-yl (4-benzyloxy)phenylcarbamate, or (3aR,8aS)-5-methoxy-1,3a,8-trimethyl-1,2,3,3a,8,8a-hexahydropyrrolo[2,3-b]indole, wherein the compound inhibits production of amyloid precursor protein in the mammalian cell.

56. A method of inhibiting production of amyloid precursor protein in a mammalian cell, comprising contacting the mammalian cell with a compound of claim 22, wherein the compound inhibits production of amyloid precursor protein in the mammalian cell.

57. A method of inhibiting production of amyloid precursor protein in a mammalian cell, comprising contacting the mammalian cell with a compound of claim 23, wherein the compound inhibits production of amyloid precursor protein in the mammalian cell.

58. A method of inhibiting production of amyloid precursor protein in a mammalian cell, comprising contacting the mammalian cell with a compound of claim 24, wherein the compound inhibits production of amyloid precursor protein in the mammalian cell.

59. A method of inhibiting production of amyloid precursor protein in a mammalian cell, comprising contacting the mammalian cell with a compound of claim 25, wherein the compound inhibits production of amyloid precursor protein in the mammalian cell.

60. A method of inhibiting production of amyloid precursor protein in a mammalian cell, comprising contacting the mammalian cell with a compound of claim 33, wherein the compound inhibits production of amyloid precursor protein in the mammalian cell.

61. A method of inhibiting production of amyloid precursor protein in a mammalian cell, comprising contacting the mammalian cell with a compound of claim 34, wherein the compound inhibits production of amyloid precursor protein in the mammalian cell.

62. A method of inhibiting production of amyloid precursor protein in a mammalian cell, comprising contacting the mammalian cell with a compound of claim 45, wherein the compound inhibits production of amyloid precursor protein in the mammalian cell.

63. A method of inhibiting production of amyloid precursor protein in a mammalian cell, comprising contacting the mammalian cell with a compound of claim 51, wherein the compound inhibits production of amyloid precursor protein in the mammalian cell.

64. A method of inhibiting production of amyloid precursor protein in a mammalian cell, comprising contacting the mammalian cell with a compound of claim 52, wherein the compound inhibits production of amyloid precursor protein in the mammalian cell.

65. A method of inhibiting production of amyloid precursor protein in a mammalian cell, comprising contacting the mammalian cell with a compound of claim 53, wherein the compound inhibits production of amyloid precursor protein in the mammalian cell.

66. The method of claim 54, wherein the cell is in vivo.

67. The method of claim 55, wherein the cell is in vivo.

68. The method of claim 60, wherein the cell is in vivo.

69. The method of claim 61, wherein the cell is in vivo.

70. The method of claim 62, wherein the cell is in vivo.

71. The method of claim 65, wherein the cell is in vivo.

72. The method of claim 54, wherein the cell is in vitro.

73. The method of claim 54, wherein the cell is a human cell.

74. The method of claim 55, wherein the cell is a human cell.

75. The method of claim 60, wherein the cell is a human cell.

76. The method of claim 61, wherein the cell is a human cell.

77. The method of claim 62, wherein the cell is a human cell.

78. The method of claim 65, wherein the cell is a human cell.

79. A method of inhibiting production of amyloid precursor protein in a mammalian subject, comprising administering to the mammalian subject an effective amount of the compound comprising formula I



wherein R1 and R2 are, independently, hydrogen, branched or straight chain C1–C8 alkyl, substituted or unsubstituted aryl, or aralkyl;
R3 is branched or straight chain C1–C4 alkyl or heteroalkyl or C4–C8 alkyl or heteroakyl, or substituted or unsubstituted aryl;
X and Y are, independently, O, S, alkyl, hydrocarbon moiety, C(H)R4, or NR5, wherein R4 and R5 are, independently, hydrogen, oxygen, branched or straight chain C1–C8 alkyl, C2–C8 alkenyl, or C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl;
wherein the compound is the substantially pure (+)-enantiomer,
in a pharmaceutically acceptable carrier, wherein the compound inhibits production of amyloid precursor protein in the mammalian subject.

80. A method of inhibiting production of amyloid precursor protein in a mammalian subject, comprising administering to the mammalian subject an effective amount of a compound comprising (+)-phenserine, (+)-cymserine, (+)-N1-phenethylnorcymserine, (+)-N1,N8-bisnorcymserine, (+)-N1, N8-bisbenzylnorphenserine, (−)-N8-benzylnorphenserine, (3aS,8aS)-1-benzyl-3a,8-dimethyl-1,2,3,3a,8,8a-hexahydropyrrolo[2,3-b]indol-5-yl (4-benzyloxy)phenylcarbamate, or (3aR,8aS)-5-methoxy-1,3a,8-trimethyl-1,2,3,3a,8,8a-hexahydropyrrolo[2,3-b]indole, in a pharmaceutically acceptable carrier, wherein the compound inhibits production of amyloid precursor protein in the mammalian subject.

81. A method of inhibiting production of amyloid precursor protein in a mammalian subject, comprising administering to the mammalian subject an effective amount of the compound in claim 22 in a pharmaceutically acceptable carrier, wherein the compound inhibits production of amyloid precursor protein in the mammalian subject.

82. A method of inhibiting production of amyloid precursor protein in a mammalian subject, comprising administering to the mammalian subject an effective amount of the compound in claim 23 in a pharmaceutically acceptable carrier, wherein the compound inhibits production of amyloid precursor protein in the mammalian subject.

83. A method of inhibiting production of amyloid precursor protein in a mammalian subject, comprising administering to the mammalian subject an effective amount of the compound in claim 24 in a pharmaceutically acceptable carrier, wherein the compound inhibits production of amyloid precursor protein in the mammalian subject.

84. A method of inhibiting production of amyloid precursor protein in a mammalian subject, comprising administering to the mammalian subject an effective amount of the compound in claim 25 in a pharmaceutically acceptable carrier, wherein the compound inhibits production of amyloid precursor protein in the mammalian subject.

85. A method of inhibiting production of amyloid precursor protein in a mammalian subject, comprising administering to the mammalian subject an effective amount of the compound in claim 33 in a pharmaceutically acceptable carrier, wherein the compound inhibits production of amyloid precursor protein in the mammalian subject.

86. A method of inhibiting production of amyloid precursor protein in a mammalian subject, comprising administering to the mammalian subject an effective amount of the compound in claim 34 in a pharmaceutically acceptable carrier, wherein the compound inhibits production of amyloid precursor protein in the mammalian subject.

87. A method of inhibiting production of amyloid precursor protein in a mammalian subject, comprising administering to the mammalian subject an effective amount of the compound in claim 45 in a pharmaceutically acceptable carrier, wherein the compound inhibits production of amyloid precursor protein in the mammalian subject.

88. A method of inhibiting production of amyloid precursor protein in a mammalian subject, comprising administering to the mammalian subject an effective amount of the compound in claim 52 in a pharmaceutically acceptable carrier, wherein the compound inhibits production of amyloid precursor protein in the mammalian subject.

89. A method of inhibiting production of amyloid precursor protein in a mammalian subject, comprising administering to the mammalian subject an effective amount of the compound in claim 53 in a pharmaceutically acceptable carrier, wherein the compound inhibits production of amyloid precursor protein in the mammalian subject.

90. A method of inhibiting production of amyloid precursor protein in a mammalian subject, comprising administering to the mammalian subject an effective amount of the compound in claim 54 in a pharmaceutically acceptable carrier, wherein the compound inhibits production of amyloid precursor protein in the mammalian subject.

91. The method of claim 79, wherein the mammal is a human.

92. The method of claim 80, wherein the mammal is a human.

93. The method of claim 85, wherein the mammal is a human.

94. The method of claim 86, wherein the mammal is a human.

95. The method of claim 87, wherein the mammal is a human.

96. The method of claim 90, wherein the mammal is a human.

97. The method of claim 79, wherein the dosage of the compound is from about 1 mg/kg to about 100 mg/kg of body weight.

98. The method of claim 80, wherein the dosage of the compound is from about 1 mg/kg to about 100 mg/kg of body weight.

99. The method of claim 85, wherein the dosage of the compound is from about 1 mg/kg to about 100 mg/kg of body weight.

100. The method of claim 86, wherein the dosage of the compound is from about 1 mg/kg to about 100 mg/kg of body weight.

101. The method of claim 87, wherein the dosage of the compound is from about 1 mg/kg to about 100 mg/kg of body weight.

102. The method of claim 90, wherein the dosage of the compound is from about 1 mg/kg to about 100 mg/kg of body weight.

103. A method of treating dementia in a subject, comprising administering to the subject an effective amount of a compound comprising formula I



wherein R1 and R2 are, independently, hydrogen, branched or straight chain C1–C8 alkyl, substituted or unsubstituted aryl, or aralkyl;
R3 is branched or straight chain C1–C4 alkyl or heteroalkyl or C4–C8 alkyl or heteroakyl, or substituted or unsubstituted aryl;
X and Y are, independently, O, S, alkyl, hydrocarbon moiety, C(H)R4, or NR5, wherein R4 and R5 are, independently, hydrogen, oxygen, branched or straight chain C1–C8 alkyl, C2–C8 alkenyl, or C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl;
wherein the compound is the substantially pure (+)-enantiomer, in a pharmaceutically acceptable carrier,
wherein the compound treats dementia in the subject.

104. A method of treating dementia in a subject, comprising administering to the subject an effective amount of a compound comprising (+)-phenserine, (+)-cymserine, (+)-N1-phenethylnorcymserine, (+)-N1, N8-bisnorcymserine, (+)-N1, N8-bisbenzylnorphenserine, (−)-N8-benzylnorphenserine, (3aS,8aS)-1-benzyl-3a,8-dimethyl-1,2,3,3a,8,8a-hexahydropyrrolo[2,3-b]indol-5-yl (4-benzyloxy)phenylcarbamate, or (3aR,8aS)-5-methoxy-1,3a,8-trimethyl-1,2,3,3a,8,8a-hexahydropyrrolo[2,3-b]indole, in a pharmaceutically acceptable carrier, wherein the compound treats dementia in the subject.

105. A method of treating dementia in a subject, comprising administering to the subject an effective amount of the compound in claim 22 in a pharmaceutically acceptable carrier, wherein the compound treats dementia in the subject.

106. A method of treating dementia in a subject, comprising administering to the subject an effective amount of the compound in claim 23 in a pharmaceutically acceptable carrier, wherein the compound treats dementia in the subject.

107. A method of treating dementia in a subject, comprising administering to the subject an effective amount of the compound in claim 24 in a pharmaceutically acceptable carrier, wherein the compound treats dementia in the subject.

108. A method of treating dementia in a subject, comprising administering to the subject an effective amount of the compound in claim 25 in a pharmaceutically acceptable carrier, wherein the compound treats dementia in the subject.

109. A method of treating dementia in a subject, comprising administering to the subject an effective amount of the compound in claim 33 in a pharmaceutically acceptable carrier, wherein the compound treats dementia in the subject.

110. A method of treating dementia in a subject, comprising administering to the subject an effective amount of the compound in claim 34 in a pharmaceutically acceptable carrier, wherein the compound treats dementia in the subject.

111. A method of treating dementia in a subject, comprising administering to the subject an effective amount of the compound in claim 51 in a pharmaceutically acceptable carrier, wherein the compound treats dementia in the subject.

112. A method of treating dementia in a subject, comprising administering to the subject an effective amount of the compound in claim 52 in a pharmaceutically acceptable carrier, wherein the compound treats dementia in the subject.

113. A method of treating dementia in a subject, comprising administering to the subject an effective amount of the compound in claim 53 in a pharmaceutically acceptable carrier, wherein the compound treats dementia in the subject.

114. A method of treating dementia in a subject, comprising administering to the subject an effective amount of the compound in claim 54 in a pharmaceutically acceptable carrier, wherein the compound treats dementia in the subject.

115. The method of claim 103, wherein the dementia is Alzheimer's Disease.

116. The method of claim 104, wherein the dementia is Alzheimer's Disease.

117. The method of claim 109, wherein the dementia is Alzheimer's Disease.

118. The method of claim 110, wherein the dementia is Alzheimer's Disease.

119. The method of claim 111, wherein the dementia is Alzheimer's Disease.

120. The method of claim 114, wherein the dementia is Alzheimer's Disease.

121. The method of claim 103, wherein the dosage of the compound is from about 1 mg/kg to about 200 mg/kg.

122. The method of claim 104, wherein the dosage of the compound is from about 1 mg/kg to about 200 mg/kg.

123. The method of claim 109, wherein the dosage of the compound is from about 1 mg/kg to about 200 mg/kg.

124. The method of claim 110, wherein the dosage of the compound is from about 1 mg/kg to about 200 mg/kg.

125. The method of claim 111, wherein the dosage of the compound is from about 1 mg/kg to about 200 mg/kg.

126. The method of claim 114, wherein the dosage of the compound is from about 1 mg/kg to about 200 mg/kg.

127. The method of claim 103, wherein the compound inhibits the production of amyloid precursor protein and Aβ1-40 or Aβ1-42.

128. The method of claim 104, wherein the compound inhibits the production of amyloid precursor protein and Aβ1-40 or Aβ1-42.

129. The method of claim 105, wherein the compound inhibits the production of amyloid precursor protein and Aβ1-40 or Aβ1-42.

130. The method of claim 106, wherein the compound inhibits the production of amyloid precursor protein and Aβ1-40 or Aβ1-42.

131. The method of claim 107, wherein the compound inhibits the production of amyloid precursor protein and Aβ1-40 or Aβ1-42.

132. The method of claim 108, wherein the compound inhibits the production of amyloid precursor protein and Aβ1-40 or Aβ1-42.

133. The method of claim 109, wherein the compound inhibits the production of amyloid precursor protein and Aβ1-40 or Aβ1-42.

134. The method of claim 110, wherein the compound inhibits the production of amyloid precursor protein and Aβ1-40 or Aβ1-42.

135. The method of claim 111, wherein the compound inhibits the production of amyloid precursor protein and Aβ1-40 or Aβ1-42.

136. The method of claim 112, wherein the compound inhibits the production of amyloid precursor protein and Aβ1-40 or Aβ1-42.

137. The method of claim 113, wherein the compound inhibits the production of amyloid precursor protein and Aβ1-40 or Aβ1-42.

138. The method of claim 114, wherein the compound inhibits the production of amyloid precursor protein and Aβ1-40 or Aβ1-42.

139. A method of screening a compound for the ability to inhibit production of amyloid precursor protein in a cell, comprising
(a) contacting the cell with a compound comprising formula I




wherein R1 and R2 are, independently, hydrogen, branched or straight chain C1–C8 alkyl, substituted or unsubstituted aryl, or aralkyl;
R3 is branched or straight chain C1–C4 alkyl or heteroalkyl or C4–C8 alkyl or heteroakyl, or substituted or unsubstituted aryl;
X and Y are, independently, O, S, alkyl, hydrocarbon moiety, C(H)R4, or NR5, wherein R4 and R5 are, independently, hydrogen, oxygen, branched or straight chain C1–C8 alkyl, C2–C8 alkenyl, or C2–C8 alkynyl, aralkyl, or substituted or unsubstituted aryl;
wherein the compound is the substantially pure (+)-enantiomer; and
(b) detecting a decrease in amyloid precursor protein production in the cell as compared to the amount of amyloid precursor protein produced in a control cell not contacted with the compound, whereby decreased production of amyloid precursor protein in the cell identifies the compound as having the ability to inhibit the production of amyloid precursor protein in a cell.

140. A method of screening a compound for the ability to inhibit production of amyloid precursor protein in a cell, comprising
(a) contacting the cell with the compound of claim 33; and
(b) detecting a decrease in amyloid precursor protein production in the cell as compared to the amount of amyloid precursor protein produced in a control cell not contacted with the compound, whereby decreased production of amyloid precursor protein in the cell identifies the compound as having the ability to inhibit the production of amyloid precursor protein in a cell.

141. A method of screening a compound for the ability to inhibit production of amyloid precursor protein in a cell, comprising
(a) contacting the cell with the compound of claim 35; and
(b) detecting a decrease in amyloid precursor protein production in the cell as compared to the amount of amyloid precursor protein produced in a control cell not contacted with the compound, whereby decreased production of amyloid precursor protein in the cell identifies the compound as having the ability to inhibit the production of amyloid precursor protein in a cell.

142. A method of screening a compound for the ability to inhibit production of amyloid precursor protein in a cell, comprising
(a) contacting the cell with the compound of claim 45; and
(b) detecting a decrease in amyloid precursor protein production in the cell as compared to the amount of amyloid precursor protein produced in a control cell not contacted with the compound, whereby decreased production of amyloid precursor protein in the cell identifies the compound as having the ability to inhibit the production of amyloid precursor protein in a cell.

143. The compound of claim 33, wherein the compound comprises the chloride salt.

144. The compound of claim 35, wherein the compound comprises the formula V, wherein the compound comprises the iodide salt.

145. The compound of claim 1, wherein the compound comprises the formula V, wherein the compound comprises the tartrate salt.

146. A compound comprising (+)-phenserine tartrate.

147. A method of inhibiting production of amyloid precursor protein in a mammalian cell, comprising contacting the mammalian cell with (+)-phenserine tartrate.

148. A method of inhibiting production of amyloid precursor protein in a mammalian subject, comprising administering to the mammalian subject an effective amount of (+)-phenserine tartrate.

149. A method of treating dementia in a subject, comprising administering to the subject an effective amount of (+)-phenserine tartrate.

150. The method according to claim 54, wherein the compound comprises (+)-phenserine tartrate.

151. The method according to claim 55, wherein the compound comprises (+)-phenserine tartrate.

152. The method according to claim 79, wherein the compound comprises (+)-phenserine tartrate.

153. The method according to claim 80, wherein the compound comprises (+)-phenserine tartrate.

154. The method according to claim 103, wherein the compound comprises (+)-phenserine tartrate.

155. The method according to claim 104, wherein the compound comprises (+)-phenserine tartrate.

156. A compound comprising (+)-N1,N8-bisnorcymserine.

157. The compound of claim 156, wherein the compound is a salt.