### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] You are here: EPA Home Prevention, Pesticides & Toxic Substances Pollution Prevention & Toxics High Production Volume (HPV) Challenge High Production Volume Information System (HPVIS) Submission Report 

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# Submission Report

Submission Information

Submission ID : 25419379 Submission Name : 1H-Benzotriazole

AR Number :



## Chemical Information

CAS Number : 95-14-7 Ninth Collective Index Name : 1H-Benzotriazole



## Submitter Information

Submitter's Name : Orphan

Address : City :
State : VA

ZIP/Postal Code : Country : Type of Submitter : Company 

## Technical Point of Contact Information

Technical Point of Contact :
Title :
Phone Number :
Fax Number :
E-Mail Address : 

## Additional Technical Point of Contact Information

Technical Point of Contact :
Title :
Phone Number :
Fax Number :
E-Mail Address :



## Sponsor Information

Sponsor's Name : Orphan Chemical

Address : City :
State : VA

ZIP/Postal Code : Country : US

Phone Number :
Fax Number :

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] E-Mail Address : Type of Sponsor : Company 

## Technical Point of Contact Information

Technical Point of Contact :
Title :
Phone Number :
Fax Number :
E-Mail Address :



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored Chemical Name : 1H-Benzotriazole



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition and Other Test Substance Comments: purity not noted

Sponsored Chemical Result Type:

Test Substance Result Type:


### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed:

Method/Guideline Followed: EPA OTS 797.1400

Other Method/Guideline:

Deviations from Method/Guideline:

Species: Lepomis macrochirus

GLP: No

Analytical Monitoring: No Data

Test Type: Static

Test Vessel:

Water Media Type:

Test Concentrations: No Data

Nominal and Measured Concentrations:

Exposure Period: 96 Hours

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks:

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 25 mg/L

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Results Remarks: Median Tolerance Limit (LC50) = 27.5 ppm (48 hrs); 25 ppm (96 hrs).



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability Remarks:

Key Study Sponsor Indicator:


### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: IUCLID (2001). Food and Drug Research Laboratories, Study No. 90801, December 1, 1969.



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored Chemical Name : 1H-Benzotriazole



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition and Other Test Substance Comments:

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed: 1978

Method/Guideline Followed: Other

Other Method/Guideline: Tested in accordance with the method reported by Bathe et al. (1974)

Deviations from Method/Guideline:

Species: Oncorhynchus mykiss

GLP:

Analytical Monitoring:

Test Type:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Test Vessel: Closed

Water Media Type:

Test Concentrations: Nominal

Nominal and Measured Concentrations: 15, 21, 37 and 65 ppm

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units: Value/Lower Range: 12 Upper Range: 16 °C

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Method/guideline followed: Tested in accordance with the method reported by Bathe et
al. (1974) which is a modification of the procedure proposed by the United States Department of the Interior Fish and Wildlife Service, reported in "Procedure for Evaluation of Acute Toxicity of Pesticides to Fish and Wildlife" of December 14, 1964. Prior to testing the food was withdrawn for 3 days. All fish were in good condition
and free from disease. The fish were tested in glass tanks, of 12 and 15 litre capacity. Testing was performed in reconstituted water prepared of deionised water to which salts were added in the following amount per litre: 30 mg calcium sulphate, 30 mg magnesium sulphate, 48 mg sodium bicarbonate, 3 mg potassium chloride.
The fish were tested at 14 ± 2°C The water was not aerated during testing. Two to five fish were placed in each tank. Various concentrations of TK 10637 were prepared by adding the test material diluted in aqua dest. (stock solution) at the appropriate amount ensuring that the total
volume in each vessel remained the same.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 23.9 ppm

Results Remarks: The test material has a slight acute toxicity to trout Rainbow trout = 23.9 (17.6-29.9) ppm, LC 50 including 95% confidence limits were calculated by the logit model. Concentrations were expressed as weight of substance per volume of water, in parts per million (ppm). These values based upon nominal
concentrations of TK 10637.


### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability Remarks:

Key Study Sponsor Indicator:


### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Acute toxicity to rainbow trout, carp, catfish and bluegill of TK 10, 637 with cover
letter dated 072893. Submitting Organization: Ciba-Geigy Corporation. OTS0538215.



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored Chemical Name : 1H-Benzotriazole



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition and Other Test Substance Comments: Purity not noted

Sponsored

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Chemical Result Type:

Test Substance Result Type:


### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed:

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline:

Species: Lebistes reticulatus

GLP: No Data

Analytical Monitoring: No Data

Test Type: Flow-through

Test Vessel:

Water Media Type:

Test Concentrations: No Data

Nominal and Measured Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Remarks:

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % 28 mg/L

Results Remarks:



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability Remarks: Lacking number of fish tested, concentrations tested and study details. (2 = valid with restrictions - per IUCLID Dataset.)

Key Study Sponsor Indicator:


### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: IUCLID (2001). Davis, L.N. et al.: Investigation of Selected Potential Environmental Contaminants. Benzotriazoles. EPA 560/2-77-001, 137-138 (1977).



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored Chemical Name : 1H-Benzotriazole

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition and Other Test Substance Comments:

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed:

Method/Guideline Followed:

Other Method/Guideline:

Deviations from Method/Guideline:

Species: Oncorhynchus mykiss

GLP:

Analytical Monitoring:

Test Type: Static

Test Vessel:

Water Media Type: Freshwater

Test Concentrations:

Nominal and Measured Concentrations: 5 ppm (5,000 μg/L)

Exposure Period: 24 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:

Alkalinity:

Dissolved Oxygen: 8.6-13.7 ppm

pH Value: 7.5 Upper Range: 8.2

Test Temperature and Units: Value/Lower Range: 55 °F

Photo

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Rainbow Trout fingerlings, ≤ 4 inches The test article was introduced at a concentration of 5,000 μg/L. The test media was fresh water, temperature was kept at 55°F, and pH ranged from 7.5-8.2, dissolved oxygen ranged from 8.6-13.7 ppm, 4 fish were used for testing.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

Results Remarks: No effects were observed at 5 ppm.



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability Remarks:

Key Study Sponsor Indicator:


### Reference - Acute Toxicity To Aquatic Vertebrates

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Reference: ECOTOX (2010). 638 Applegate, V.C., J.H. Howell, A.E. Hall Jr., and M.A. Smith, 1957. Title: Toxicity of 4,346 Chemicals to Larval Lampreys and Fishes. Special Scientific Report- -Fisheries No. 207. March 1957.



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored Chemical Name : 1H-Benzotriazole



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition and Other Test Substance Comments:

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed: 1978

Method/Guideline Followed: Other

Other Method/Guideline: Tested in accordance with the method reported by Bathe et al. (1974)

Deviations from Method/Guideline:

Species: Cyprinus carpio

GLP:

Analytical Monitoring:

Test Type:

Test Vessel: Closed

Water Media Type:

Test Concentrations: Nominal

Nominal and Measured Concentrations: 8.7, 21, 37, 49 and 65 ppm

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Vehicle Amount and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units: Value/Lower Range: 20 Upper Range: 22 °C

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Method/guideline followed: Tested in accordance with the method reported by Bathe et
al. (1974) which is a modification of the procedure proposed by the United States Department of the Interior Fish and Wildlife Service, reported in "Procedure for Evaluation of Acute Toxicity of Pesticides to Fish and Wildlife" of December 14, 1964. Test method/conditions remarks: Prior to testing the food was withdrawn for 3 days. All fish were in good condition and free from disease. The fish were tested in glass tanks, of 12 and 15 litre capacity. Testing was performed in reconstituted water prepared of deionised water to which salts were added in the following amount per litre: 30 mg calcium sulphate, 30 mg magnesium sulphate, 48 mg sodium bicarbonate, 3 mg potassium chloride. The fish were tested at 14 ± 2°C (trout, bluegill) and at 21 ± 1°C (carp, catfish). The water was not aerated during testing. Two to five fish were placed in
each tank. Various concentrations of TK 10637 were prepared by adding the test material diluted in aqua dest. (stock solution) at the appropriate amount ensuring that the total
volume in each vessel remained the same.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Upper Mean Value Units Effect Observed Basis for Concentration

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Value

96 Hours LC 50 % = 30 ppm

Results Remarks: The test material has a slight acute toxicity to tout and carp and is practically devoid of acute toxicity to catfish and bluegill when tested and evaluated according to the procedure of Bathe et al (1974).


### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability Remarks:

Key Study Sponsor Indicator:


### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Acute toxicity to rainbow trout, carp, catfish and bluegill of TK 10, 637 with cover
letter dated 072893. Submitting Organization: Ciba-Geigy Corporation. OTS0538215.



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored Chemical Name : 1H-Benzotriazole



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition and Other Test Substance Comments:

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed:

Method/Guideline Followed:

Other Method/Guideline:

Deviations from Method/Guideline:

Species: Oncorhynchus kisutch

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] GLP:

Analytical Monitoring: No

Test Type: Static

Test Vessel:

Water Media Type: Freshwater

Test Concentrations:

Nominal and Measured Concentrations: 10 ppm (10,000 μg/L)

Exposure Period: 24 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:

Alkalinity: 7 mg/L CaCO3

Dissolved Oxygen:

pH Value: Value/Lower Range: = 7.2

Test Temperature and Units: Value/Lower Range: 12 °C

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness: 7 mg/L

Method/Guideline Test Conditions Remarks: The test article was introduced at a concentration of 10,000 ìg/L. The test media was fresh water, temperature was kept at 12°C, and pH at 7.2, the water hardness was 7 mg/L CaCO3, and the alkalinity was 7 mg/L CaCO3. Polyethylene bags were used as the test vessel and aerated, 23 fish were used for testing.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

Results Remarks: Neither loss of equilibrium nor death occurred.



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability Remarks:

Key Study Sponsor Indicator:


### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: ECOTOX (2010). 15148 MacPhee, C., and R. Ruelle, 1969. Lethal Effects of 1888 Chemicals Upon Four Species of Fish from Western North America. Bull. No. 3, Forest, WildI. And Range Exp. Stn., Univ of Idaho, Moscow, ID: 112p.



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored Chemical Name : 1H-Benzotriazole



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition and Other Test Substance Comments:

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Year Study Performed: 1969

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline:

Species: Other

Other Species: Trout

GLP: No

Analytical Monitoring: Yes

Test Type: Flow-through

Test Vessel:

Water Media Type: Freshwater

Test Concentrations:

Nominal and Measured Concentrations: In order to estimate the concentration to be used in the dynamic system, a series of static tests were performed using 0.1, 1.0, 10, 30, 50 and 100 ppm BT-D.

For dynamic test, concentrations were 10, 12, 14 and 16 ppm

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Trout were maintained for 96 hours prior to use at 55-60oF in specially designed dynamic fresh water recirculating tanks of 100-gallon capacity, were fed a dry commercial fish flake until 24 hours prior to initiation of testing.

Limit Test:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

48 Hours LC 50 % 15 ppm Mortality Estimated

96 Hours LC 50 % 12 ppm Mortality Estimated

Results Remarks: TLm values were 15 and 12 ppm at 48 and 96 hours, respectively. LC50 values were 15 and 12 ppm at 48 and 96 hours, respectively


### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability Remarks:

Key Study Sponsor Indicator:


### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: The effects of BT-D on trout, bluegill and fathead minnow fingerlings with attachments
and cover letter dated 061289. Submitting Organization: Sherwin Williams Company. Performing Laboratory: Food and Drug Research Labs. OTS0520640



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored Chemical Name : 1H-Benzotriazole



### Test Substance - Acute Toxicity To Aquatic Vertebrates

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition and Other Test Substance Comments:

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed: 1969

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline:

Species: Lepomis macrochirus

GLP: No

Analytical Monitoring: Yes

Test Type: Static

Test Vessel:

Water Media Type: Freshwater

Test Concentrations:

Nominal and Measured Concentrations: 22, 24, 26 and 28 ppm

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):

Salinity:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Bluegills were maintained in 100 gallon tanks for 96 hours at 68 oF, were fed a dry commercial fish flake until 24 hours prior to initiation of testing.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

48 Hours LC 50 % 27.5 ppm Mortality Estimated

96 Hours LC 50 % 25 ppm Mortality Estimated

Results Remarks: TLm values were 27.5 and 25.0 ppm at 48 and 96 hours, respectively. LC50 values were 27.5 and 25.0 ppm at 48 and 96 hours, respectively.



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability Remarks:

Key Study Sponsor Indicator:


### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: The effects of BT-D on trout, bluegill and fathead minnow fingerlings with attachments
and cover letter dated 061289. Submitting Organization: Sherwin Williams Company. Performing Laboratory: Food and Drug Research Labs. OTS0520640

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] 

## Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored Chemical Name : 1H-Benzotriazole



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition and Other Test Substance Comments:

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed: 1978

Method/Guideline Followed: Other

Other Method/Guideline: Tested in accordance with the method reported by Bathe et al. (1974)

Deviations from Method/Guideline:

Species: Lepomis macrochirus

GLP:

Analytical Monitoring:

Test Type:

Test Vessel: Closed

Water Media Type:

Test Concentrations: Nominal

Nominal and Measured Concentrations: 10 and 100 ppm

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:

Alkalinity:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Dissolved Oxygen:

pH Value:

Test Temperature and Units: Value/Lower Range: 20 Upper Range: 22 °C

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Method/guideline followed: Tested in accordance with the method reported by Bathe et
al. (1974) which is a modification of the procedure proposed by the United States Department of the Interior Fish and Wildlife Service, reported in "Procedure for Evaluation of Acute Toxicity of Pesticides to Fish and Wildlife" of December 14, 1964. Test method/conditions remarks: Prior to testing the food was withdrawn for 3 days. All fish were in good condition and free from disease. The fish were tested in glass tanks, of 12 and 15 litre capacity. Testing was performed in reconstituted water prepared of deionised water to which salts were added in the following amount per litre: 30 mg calcium sulphate, 30 mg magnesium sulphate, 48 mg sodium bicarbonate, 3 mg potassium chloride. The fish were tested at 14 ± 2°C (trout, bluegill) and at 21 ± 1°C (carp, catfish). The water was not aerated during testing. Two to five fish were placed in
each tank. Various concentrations of TK 10637 were prepared by adding the test material diluted in aqua dest. (stock solution) at the appropriate amount ensuring that the total
volume in each vessel remained the same.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % > 100 ppm

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Results Remarks: The test material has a slight acute toxicity to tout and carp and is practically devoid of acute toxicity to catfish and bluegill when tested and evaluated according to the procedure of Bathe et al (1974).


### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability Remarks:

Key Study Sponsor Indicator:


### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Acute toxicity to rainbow trout, carp, catfish and bluegill of TK 10, 637 with cover
letter dated 072893. Submitting Organization: Ciba-Geigy Corporation. OTS0538215.



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored Chemical Name : 1H-Benzotriazole



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition and Other Test Substance Comments:

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed: 1969

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline:

Species: Pimephales promelas

GLP: No

Analytical Monitoring: Yes

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Test Type: Flow-through

Test Vessel:

Water Media Type: Freshwater

Test Concentrations:

Nominal and Measured Concentrations: 26, 28, 30, 32 and 34 ppm

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Fathead minnows were maintained in 100 gallon tanks for 96 hours at 68 oF, were fed a dry commercial fish flake until 24 hours prior to initiation of testing.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

48 Hours LC 50 % 30.2 ppm Mortality Estimated

96 Hours LC 50 % 28 ppm Mortality Estimated

Results Remarks: TLm values were 30.2 and 28.0 ppm at 48 and 96 hours, respectively. LC50 values were 30.2 and 28.0 ppm at 48 and 96 hours, respectively.



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability Remarks:

Key Study Sponsor Indicator:


### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: The effects of BT-D on trout, bluegill and fathead minnow fingerlings with attachments
and cover letter dated 061289. Submitting Organization: Sherwin Williams Company. Performing Laboratory: Food and Drug Research Labs. OTS0520640



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored Chemical Name : 1H-Benzotriazole



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition and Other Test Substance Comments:

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed: 1969

Method/Guideline Followed: No Data

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Other Method/Guideline:

Deviations from Method/Guideline:

Species: Lepomis macrochirus

GLP: No

Analytical Monitoring: Yes

Test Type: Flow-through

Test Vessel:

Water Media Type: Freshwater

Test Concentrations:

Nominal and Measured Concentrations: 26, 28, 30, 32 and 34 ppm

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Bluegills were maintained in 100 gallon tanks for 96 hours at 68 oF, were fed a dry commercial fish flake until 24 hours prior to initiation of testing.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

48 Hours LC 50 % 27.5 ppm Mortality Estimated

96 Hours LC 50 % 25 ppm Mortality Estimated

Results Remarks: TLm values were 27.5 and 25.0 ppm at 48 and 96 hours, respectively. LC50 values were 27.5 and 25.0 ppm at 48 and 96 hours, respectively.



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability Remarks:

Key Study Sponsor Indicator:


### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: The effects of BT-D on trout, bluegill and fathead minnow fingerlings with attachments
and cover letter dated 061289. Submitting Organization: Sherwin Williams Company. Performing Laboratory: Food and Drug Research Labs. OTS0520640



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored Chemical Name : 1H-Benzotriazole



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition and Other Test Substance Comments:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Sponsored Chemical Result Type: Measured

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed: 1969

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline:

Species: Pimephales promelas

GLP: No

Analytical Monitoring: Yes

Test Type: Static

Test Vessel:

Water Media Type: Freshwater

Test Concentrations:

Nominal and Measured Concentrations: 22, 24, 26 and 28 ppm

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Method/Guideline Test Conditions Remarks: Fathead minnows were maintained in 100 gallon tanks for 96 hours at 68 oF, were fed a dry commercial fish flake until 24 hours prior to initiation of testing.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

48 Hours LC 50 % 30.2 ppm Mortality Estimated

96 Hours LC 50 % 28 ppm Mortality Estimated

Results Remarks: TLm values were 30.2 and 28.0 ppm at 48 and 96 hours, respectively. LC50 values were 30.2 and 28.0 ppm at 48 and 96 hours, respectively.



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability Remarks:

Key Study Sponsor Indicator:


### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: The effects of BT-D on trout, bluegill and fathead minnow fingerlings with attachments
and cover letter dated 061289. Submitting Organization: Sherwin Williams Company. Performing Laboratory: Food and Drug Research Labs. OTS0520640



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Sponsored Chemical Name : 1H-Benzotriazole



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition and Other Test Substance Comments: Substance type: organic Physical status: solid Purity: ca. 100 % w/w

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed: 1984

Method/Guideline Followed: Other

Other Method/Guideline: Letale Wirkung beim Zebrabaerbling - Brachydanio rerio

Deviations from Method/Guideline:

Species: Danio rerio

GLP: No Data

Analytical Monitoring: No

Test Type: Static

Test Vessel:

Water Media Type:

Test Concentrations:

Nominal and Measured Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:

Alkalinity:

Dissolved Oxygen:

pH Value: 6.5 Upper Range: 7

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Test Temperature and Units: Value/Lower Range: 19.6 Upper Range: 23 °C

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: "Letale Wirkung beim Zebrabaerbling - Brachydanio rerio" (LC 0, LC 50, LC 100; 48-96 Stunden), Verfahrensvorschlag Umweltbundesamt, Mai 1984

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % > 100 mg/L

Results Remarks:



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability Remarks: 4 = not assignable. Lacking number of fish tested, concentrations tested and study
details. (2 = valid with restrictions Meets generally accepted scientific standards, well documented and acceptable for assessment - per IUCLID Dataset.)

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Key Study Sponsor Indicator:


### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: IUCLID (2001). Bayer report Prof. Caspers in 1985-08-03.



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored Chemical Name : 1H-Benzotriazole



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition and Other Test Substance Comments: Cobratec 99; purity >99%

Sponsored Chemical Result Type: Estimated by Calculation

Test Substance Result Type: Estimated



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed:

Method/Guideline Followed: Other

Other Method/Guideline: Batelle Protocol

Deviations from Method/Guideline:

Species: Salmo sp.

GLP: No Data

Analytical Monitoring: No Data

Test Type: No Data

Test Vessel:

Water Media Type:

Test Concentrations:

Nominal and Measured Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Vehicle Name:

Vehicle Amount and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks:

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 39 mg/L

96 Hours EC 50 % = 24.4 mg/L

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Results Remarks: LC50 generated by Binomial method; 95% confidence limits = 28.4- 49.5 mg/L EC50 generated by Binomial method; 95% confidence limits = 21.0- 28.4 mg/L.



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability Remarks: Lacking number of fish tested, concentrations tested and study details. (2 = valid with restrictions Meets generally accepted scientific standards, well documented and acceptable for assessment - per IUCLID Dataset)

Key Study Sponsor Indicator:


### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: IUCLID (2001). Battelle Study. 10/1/85; PMC Specialties Group, Inc. MSDS.



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored Chemical Name : 1H-Benzotriazole



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition and Other Test Substance Comments: Purity not noted

Sponsored Chemical Result Type:

Test Substance Result Type:


### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed:

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline:

Species: Pimephales promelas

GLP: No Data

Analytical Monitoring: No Data

Test Type: Flow-through

Test Vessel:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Water Media Type:

Test Concentrations: No Data

Nominal and Measured Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks:

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Upper Mean Value Units Effect Observed Basis for Concentration

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Lower Mean Value

96 Hours LC 50 % 28 mg/L

Results Remarks:



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability Remarks: Lacking number of fish tested, concentrations tested and study details. (2 = valid with restrictions - per IUCLID Dataset.)

Key Study Sponsor Indicator:


### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: IUCLID (2001). Davis, L.N. et al.: Investigation of Selected Potential Environmental Contaminants. Benzotriazoles. EPA 560/2-77-001, 137-138 (1977).



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored Chemical Name : 1H-Benzotriazole



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition and Other Test Substance Comments: purity not noted

Sponsored Chemical Result Type:

Test Substance Result Type:


### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed:

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline:

Species: Pimephales promelas

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] GLP: No Data

Analytical Monitoring: No Data

Test Type: Static

Test Vessel:

Water Media Type:

Test Concentrations: No Data

Nominal and Measured Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks:

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 25 mg/L

Results Remarks:



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability Remarks: Lacking number of fish tested, concentrations tested and study details. (2 = valid with restrictions - per IUCLID Dataset.)

Key Study Sponsor Indicator:


### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: IUCLID (2001). Davis, L.N. et al.: Investigation of Selected Potential Environmental Contaminants. Benzotriazoles. EPA 560/2-77-001, 137-138 (1977).



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored Chemical Name : 1H-Benzotriazole



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition and Other Test Substance Comments: purity not noted

Sponsored Chemical Result Type:

Test Substance Result Type:


### Method - Acute Toxicity To Aquatic Vertebrates

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Year Study Performed:

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline:

Species: Lepomis macrochirus

GLP: No Data

Analytical Monitoring: No Data

Test Type: Static

Test Vessel:

Water Media Type:

Test Concentrations: No Data

Nominal and Measured Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks:

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 25 mg/L

Results Remarks:



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability Remarks: Lacking number of fish tested, concentrations tested and study details. (2 = valid with restrictions - per IUCLID Dataset.)

Key Study Sponsor Indicator:


### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: IUCLID (2001). Davis, L.N. et al.: Investigation of Selected Potential Environmental Contaminants. Benzotriazoles. EPA 560/2-77-001, 137-138 (1977).



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored Chemical Name : 1H-Benzotriazole



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition purity not noted

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] and Other Test Substance Comments:

Sponsored Chemical Result Type:

Test Substance Result Type:


### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed:

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline:

Species: Lepomis macrochirus

GLP: No

Analytical Monitoring: No Data

Test Type: Flow-through

Test Vessel:

Water Media Type:

Test Concentrations: No Data

Nominal and Measured Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):

Salinity:

TOC:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Water Hardness:

Method/Guideline Test Conditions Remarks:

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 28 mg/L

Results Remarks: Median Tolerance Limit (LC50) = 30.2 ppm (48 hrs); 28.0 ppm (96 hrs).



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability Remarks:

Key Study Sponsor Indicator:


### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: IUCLID (2001). Food and Drug Research Laboratories, Study No. 90801, December 1, 1969.



## Acute Toxicity to Aquatic Vertebrates

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] CAS Number : 95-14-7

Sponsored Chemical Name : 1H-Benzotriazole



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition and Other Test Substance Comments: purity not noted

Sponsored Chemical Result Type:

Test Substance Result Type:


### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed:

Method/Guideline Followed: No Data

Other Method/Guideline:

Deviations from Method/Guideline:

Species: Oncorhynchus mykiss (Fish, estuary, fresh water)

GLP: No

Analytical Monitoring: No Data

Test Type: Flow-through

Test Vessel:

Water Media Type:

Test Concentrations: No Data

Nominal and Measured Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Test Temperature and Units:

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Test species: Rainbow trout, Salmo gairdneri (Fish, estuary, fresh water)

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 12 ppm

48 Hours LC 50 % = 15 ppm

Results Remarks: LC50 = 15 ppm (48 hrs); 12 ppm (96 hrs).



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability Remarks:

Key Study Sponsor Indicator:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Reference - Acute Toxicity To Aquatic Vertebrates

Reference: IUCLID (2001). Food and Drug Research Laboratories, Study No. 90801, December 1, 1969.



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored Chemical Name : 1H-Benzotriazole



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition and Other Test Substance Comments: purity not noted

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed: 1957

Method/Guideline Followed:

Other Method/Guideline:

Deviations from Method/Guideline:

Species: Lepomis macrochirus

GLP:

Analytical Monitoring: No

Test Type: Static

Test Vessel:

Water Media Type:

Test Concentrations:

Nominal and Measured Concentrations: The test article was introduced at a one time concentration of 5000 μg/L.

Exposure Period: 24 Hours

Vehicle Used:

Vehicle Name:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Vehicle Amount and Units:

Alkalinity:

Dissolved Oxygen:

pH Value: 7.5 Upper Range: 8.2

Test Temperature and Units: Value/Lower Range: 55 °F

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Test species: Bluegill sunfish (Lepomis macrochirus), 2 fish used, fingerlings, ≤ 4 inches The test article was introduced at a one time concentration of 5000 μg/L. The test media was fresh water, temperature was kept at 55°F, and pH ranged from 7.5-
8.2.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % > 5 ppm

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Results Remarks: No effects were observed at 5 ppm.



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability Remarks:

Key Study Sponsor Indicator:


### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: ECOTOX (2010). 638 Applegate, V.C., J.H. Howell, A.E. Hall Jr., and M.A. Smith, 1957. Title: Toxicity of 4,346 Chemicals to Larval Lampreys and Fishes. Special Scientific Report- -Fisheries No. 207. March 1957.



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored Chemical Name : 1H-Benzotriazole



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition and Other Test Substance Comments:

Sponsored Chemical Result Type: Measured

Test Substance Result Type: Measured



### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed:

Method/Guideline Followed:

Other Method/Guideline:

Deviations from Method/Guideline:

Species: Oncorhynchus tschawytscha

GLP:

Analytical Monitoring: No

Test Type: Static

Test Vessel:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Water Media Type: Freshwater

Test Concentrations:

Nominal and Measured Concentrations: 10 ppm (10,000 μg/L)

Exposure Period: 24 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:

Alkalinity: 7 mg/L CaCO3

Dissolved Oxygen:

pH Value: 7.2

Test Temperature and Units: Value/Lower Range: 12 °C

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness: 0 - 17 mg/L

Method/Guideline Test Conditions Remarks: The test article was introduced at a concentration of 10,000 μg/L. The test media was fresh water, temperature was kept at 12°C with a pH of 7.2, hardness of 0-17 mg/L CaCO3 and an alkalinity of 7 mg/L CaCO3. Polyethylene bags were used as the test vessel, 22 fish were used for testing.

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Exposure LC/EC % Value Mean Upper Units Effect Basis for

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Duration Units Description Value

or Lower Mean Value Mean Value Observed Concentration

Results Remarks: Neither loss of equilibrium nor death occurred



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability Remarks:

Key Study Sponsor Indicator:


### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: ECOTOX (2010). 15148 MacPhee, C., and R. Ruelle, 1969. Lethal Effects of 1888 Chemicals Upon Four Species of Fish from Western North America. Bull. No. 3, Forest, WildI. And Range Exp. Stn., Univ of Idaho, Moscow, ID: 112p.



## Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored Chemical Name : 1H-Benzotriazole



### Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance Purity/Composition and Other Test Substance Comments: purity not noted

Sponsored Chemical Result Type:

Test Substance Result Type:


### Method - Acute Toxicity To Aquatic Vertebrates

Year Study Performed:

Method/Guideline Followed:

Other Method/Guideline:

Deviations from Method/Guideline:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] Species: Salmo sp.

GLP: No Data

Analytical Monitoring: No Data

Test Type: Flow-through

Test Vessel:

Water Media Type:

Test Concentrations:

Nominal and Measured Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature and Units:

Photo (Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline Test Conditions Remarks: Test species: Rainbow trout, Salmo gairdneri (Fish, estuary, fresh water) Fingerlings

Limit Test:



### Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure Duration:

NOEC:

LOEC Exposure Duration:

LOEC:

### High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint [6/15/2011 10:27:28 AM] NOELR Exposure Duration:

NOELR:

LOELR Exposure Duration:

LOELR:

LC/EC Mean Value : Exposure Duration Exposure Units LC/EC % Value Description Mean Value

or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

96 Hours LC 50 % = 12 mg/L

Results Remarks:



### Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability Remarks:

Key Study Sponsor Indicator:


### Reference - Acute Toxicity To Aquatic Vertebrates

Reference: IUCLID (2001). Davis, L.N. et al.: Investigation of Selected Potential Environmental Contaminants. Benzotriazoles. EPA 560/2-77-001, 137-138 (1977).