### UNITED STATES ENVIRONMEN TAL PROTECTION AGENCY WASHINGTON, D.C. 20460

OFFICE OF PREVENTION, PESTICIDES AND TOXIC SUBSTANCES



## Date: August 15, 2007 Chemical: Acequinocyl PC Code: PC 006329 DP Barcodes: DP 337381, 330467, 330471

MEMORANDUM

SUBJECT: Transmittal Memo for the “Ecologi cal Risk Assessment for Use of Acequinocyl on Grapes, Pome Fruits , Citrus, Nuts, and Outdoor NonFood Uses (DP 337381, 330467, 330471)”

FROM: Sujatha Sankula Ph. D., Biologist Amer Al-Mudallal, Chemist and Acting Branch Chief Environmental Risk Branch I Environmental Fate and Effects Division (7507P)

TO : Marilyn Mautz Registration Division (7505P)

Attached is the Environmental Fate and Effects Division (EFED)’s ecologi cal risk assessment for the proposed use of acequinocyl (PC 006329) for food (grapes, pome fruits, citrus, and tree nuts) and non-food outdoor uses (a broad use categor y that includes various ornamentals). The proposed Section 3 request for la bel changes include addition of uses in grapes (DP 337381), extension of the listing of crops for tree nuts, pome fruits, and citrus fruit groups (DP 330467), and expansion of non-food outdoor uses to includ e forest nurseries, arboretums, public parks, rights-of-way, and recr eation areas (DP 330471).

## II Acequinocyl, the active ingredient of the insecticide Kanemite 15 SC, is used for the control of various species of mites on both food and non-food crops. Its mode of action is mediated by both the parent chemical and its primary degrad ate, 2-dodecyl-3-hydroxy-1,4-napthoquinone (also referred to as R1, deacetylated acequinocyl , acequinocyl-OH, OH- AKD 2023, or AKM-05) through the inhibition of electron transfer in mitochondria. Prim arily effective through contact and to a lesser degree through ingestion, acequinocyl pr ovides control of all life stages of mites, including eggs.

The labeled maximum application rate for acequinocyl in food crops is 0.3 lb ai/A, as a foliar spray twice per crop year, with a minimum interv al between applications of 21 days. The label states that the maximum rate per application and annually for ornamentals (other than roses) is 0.125 and 0.6 lb ai/A, respectively. Proposed applica tion rate for roses is 50% lower than that used in other ornamentals (0.06 lb ai/A). A maxi mum of five acequinocyl applications at 14 day intervals were presumed for ornamentals. Fo r both food as well as non-food uses, the label specifies that acequinocyl should be applie d by ground only and not through any irrigation system and within 75 feet of aquatic areas.

Due to uncertainties associated with the analyt ical methods used in fate studies along with storage stability issues, EFED used two approa ches (Model Approach 1 and Model Approach 2) in estimating acequinocyl exposure concentrations for aquatic organisms. In the first approach, EFED followed a conservative approach and mo deled acequinocyl alone with the assumption that the chemical is stable to all routes of degrad ation. In the second approach, EFED modeled the use of acequinocyl based on total (acequinocyl + R1) residues, with the assumption that metabolite R1 is also of toxicological concern.

In general, the current screeni ng level risk assessment for acequi nocyl suggests that the proposed food and non-food uses are above the Agency’s level of conc ern for direct acute (listed and non-listed) and chronic exposure to aquati c invertebrates and chronic exposure to terrestrial birds and mammals. In contrast, a cequinocyl uses are below the Agency’s level of concern for direct acute (listed and non-lis ted) and chronic exposure to fish, aquatic plants, and terrestrial plants and direct acute (listed and non- listed) exposure to terrestrial birds and mammals.

The conclusions can be found in the Executive Summary of the risk assessment. Specific key findings of the ecological risk assessment are summarized below:

• Non-target organisms most at risk from acequinocyl use are a quatic invertebrates near the use site(s). Regardless the use rate, frequency of application, use type (food or non-food), and model approach, acequinocyl is very highly to xic to aquatic invertebrates. Based on the toxicity end points, acequinocyl appears to be more toxic to estuarine/marine invertebrates compared to freshwater invertebrates.

• All of the proposed uses for acequinocyl pose potential acute and acute endangered species risk to aquatic invertebrate s. Although there are no fe derally listed endangered

## III estuarine/marine invertebrates at present, fres hwater invertebrates may potentially be affected by the proposed acequinocyl uses.

• The potential chronic risk to estuarine/marine invertebrates is not determined as there is no mysid life cycle study submitted. However, chronic risk to freshwater invertebrates exceeded agency’s LOC for both non-endangered and enda ngered species. In view of the greater sensitivity of estuarine/marine invertebrates compared to freshwater invertebrates, it is reasonable to assume that acequinocyl poses chr onic risk to estuarine/marine invertebrates also.

• The predicted aquatic concentrations for acequinocyl are below its solubility limit and are lower than the reported LC 50 

## values for freshwater and mari ne fish. Therefore the proposed food and non-food uses for acequinocyl do not appear to pose a potential for acute and chronic risk to fish in both fr eshwater and marine media.

• No LOC exceedances occurred on an acute basi s for birds and mammals. However, chronic dietary-based exceedances occurred for food uses only (i.e., with the 0.3 lb ai/A rate) for both birds and mammals that feed on short grass exclusively.

• Adverse effects to mammals appear to be li kely based on chronic dose-based RQs. Dosebased mammalian RQs ranged between 1 and 10.

• There are no risk concerns for acute direct effects to endangered or non-endangered terrestrial vascular and a quatic non-vascular plants.

• In view of LOC exceedances occurring even wi th the lowest non-food use rate of 0.06 lb/A for roses, reduction of application rate is not a viable option to mitigate impacts to levels below screening concern triggers.

Data Requirements

• Most of the environmental fate studies subm itted prior to the initial registration were classified ‘supplemental’ due to deficiencies identified in the fate studies such as poor chromatographic separation of acequinocyl and R1, use of chloroform : methanol solvent system, lack of LOD and LOQ data, un-eluted radioactive residues in the solid phase extraction process, and lack of storage stability data. These is sues need to be addressed by the registrant to upgrade the fate studies.

• Two method validation studies (MRID 46182601 and 46182602) submitted by the registrant to determine the concentrations of acequinocyl and its metabolite( s) in soil and water extracts were reviewed by the EPA’s Environmental Chemistry Laboratory. The registrant was informed of the suggested amendments and requ est for new calibration data in a memo dated 27 June, 2007.

## IV • The registrant submitted 5 new ecological eff ects studies since the granting of conditional registration for Kanemite SC in 2003. These st udies include 2 acute toxicity studies on freshwater invertebrates ( Daphnia magna ) (MRID 46370501 and 46370502), an acute study on freshwater fish (common carp) (DP 309568), and 2 acute (oral and dietary) studies on northern bobwhite quail (MRID 46615501 and 46622701). No new acute toxicity studies are needed on the above species.

• The lack of acequinocyl’s chronic toxicity da ta on estuarine/marine invertebrates was identified as a data gap in both the current a nd initial risk assessment. The mysid life cycle study was requested as part of the conditiona l registration granted for acequinocyl in 2003. The registrant submitted a study waiver reques t in 2006 citing that instability and poor recovery of acequinocyl in saline water posed problems in determining the chronic effects of acequinocyl to mysid shrimp. In view of the fa te profile of acequinocyl and its potential toxic effects to aquatic invertebrates, EFED did not recommend granting this waiver in a memo dated 7 June, 2007.

• Based on the potential for acequinocyl to bind to soil and toxic effects to aquatic invertebrates, EFED recommends submission of an acute toxicity test for sediment dwelling invertebrates. The acute toxicity test subm itted by the registrant on the sediment dwelling Chironomus riparius used formulated product, failed to use sediment in the water column, and was unable to establish a de finitive endpoint. Therefore, an acute toxicity test using a sediment dwelling invertebrate (using both acequinocyl and R1) is requested.

Recommended Label Changes The current Kanemite 15 SC label for ornamental s reads as follows: “For control of two-spotted spider mite ( Tetranychus urticae ), spruce spider mite ( Oligonychus ununquis ), European red mite Panonychus ulmi ), and citrus red mite ( Panonychus citric ), mix 6.4 – 12.8 fluid ounces of Kanemite 15SC in 100 gallons of water (0.06 -0 .125 lb ai/100 gal of water). Apply as a full coverage spray to foliage to th e point of drip except for rose s where the maximum application rate is 6.4 fluid ounces in 100 gallons of water (0.06 lbs ai/100 gal of water). Actual spray volume will vary depending on the size of plants being sprayed. Application should be made as soon as mite populations reach economic infestat ion levels”. Unlike the label information for food crops, the label information for ornamental s does not indicate the ti me interval between applications and maximum number of seasonal or yearly applications. EFED suggests enhancing the label clarity by including treatment interval and maximum yearly applications for ornamentals so as to prevent excessive appl ications and preserve the compatibility of acequinocyl in IPM practices.

# Ecological Risk Assessment for Use of Acequinocyl (PC 006329)

on Grapes, Pome Fruits, Citrus, Nuts , and Outdoor Non-Food Uses (DP 337381, 330467, 330471)

August 2007

## 2 

# Table of Contents

1. Executive Summary............................................................................................ 5 1.1 Nature of Stressor ................................................................................... 5 1.2 Potential Risk.......................................................................................... 6 1.3 Endangered Species Assessment ............................................................ 6

2. Problem Formulation .......................................................................................... 8 2.1 Stressor Description................................................................................ 8 2.2 Environmental Fate Summary ................................................................ 8 2.3 Mode of Action....................................................................................... 9 2.4 Use Characterization............................................................................... 9 2.5 Assessment Endpoints .......................................................................... 11 2.5.1 Ecosystems Potentially at Risk ......................................................... 11 2.5.2 Ecological Effects ............................................................................. 11 2.6 Risk Hypotheses.................................................................................... 13 2.7 Key Uncertainties and Information Gaps ............................................. 13

3. Exposure Assessment........................................................................................ 14 3.1 Label Applica tion Rates and Intervals.................................................. 14 3.2 Aquatic Exposure Estimates ................................................................. 15 3.2.1 PRZM-EXAMS Modeling Inputs and Scenario Selection ............... 15 3.2.2 PRZM-EXAMS Modeling Output.................................................... 16 3.3 Terrestrial Exposure Estimates ............................................................. 18 3.3.1 Birds and Mammals .......................................................................... 18 3.3.2 Plants................................................................................................. 21

4. Ecological Effects Assessment ......................................................................... 22 4.1 Aquatic Effects Summary..................................................................... 22 4.2 Terrestrial Effects Summary................................................................. 26 4.3 Incident Database Review..................................................................... 30

5. Risk Estimation................................................................................................. 31 5.1 Summary of Aquatic RQs..................................................................... 31 5.2 Summary of Terrestrial RQs................................................................. 33 5.2.1 Avian RQs......................................................................................... 33 5.2.2 Mammalaian RQs ............................................................................. 34 5.2.3 Plant RQs .......................................................................................... 36 5.2.4 Insects ............................................................................................... 36

## 3 6. Risk Characterization........................................................................................ 36 6.1 Aquatic Risk.......................................................................................... 37 6.1.1 Animals............................................................................................. 37 6.1.1.1 Risks Based on the Probit Slope Analysis...................38 6.1.2 Plants................................................................................................ . 39 6.2 Terrestrial Risk....................................................................................... 39 6.2.1 Animals............................................................................................. 39 6.2.1.1 Risks Based on the Probit Slope Analysis...........................40 6.2.2 Plants................................................................................................ . 40 6.3 Endangered Species .............................................................................. 40 6.3.1 Aquatic Endangered Species............................................................. 40 6.3.2 Terrestrial Endangered Species......................................................... 40 6.3.3 Direct and Indirect Effects.................................................42

References..................................................................................................................... 43 Appendix A Aquatic Exposure PRZM-EXAMS Outputs..................................44 Appendix B Terrestrial (Ani mal) Exposure TREX Inputs.................................92 Appendix C Terrestrial Plan t Exposure TERRPLANT Inputs ............................95 Appendix D Terrestrial Plant Exposure TERRPLANT Outputs.........................107 Appendix E List of Ecological Studies Submitted for Acequinocyl.....................110 Appendix F The Risk Quotient Method and Levels of Concern..........................112 Appendix G Aquatic RQ Calculations.......................................................113 Appendix H Endangered Species Summary for Acequinocyl ...........................114

## 4 List of Tables Table 1 Number of Endangered Species O ccurring in the Same St ate as Proposed Crops7 Table 2 Summary of Potential Direct and In direct Effects to Fede rally-Listed Taxa.......7 Table 3 Acequinocyl Application Information........... .................................. 15 Table 4 Assumptions for Model Approaches 1 and 2................................................. ...15 Table 5 Input Parameters for PRZM-EXAMS Modeling................................... .........16 Table 6 EECs Based on PRZM-EXAMS & Model Approach 1................................... 17 Table 7 EECs Based on PRZM-EXAMS & Model Approach 2................................... 17 Table 8 Avian Exposure Concentration Estimates for Food Uses.................................. 19 Table 9 Avian Exposure Concentration Estimates for Non-Food Uses ......................... 19 Table 10 Avian Exposure Concentration Estimates for Non-Food Uses (Roses) ............ 19 Table 11 Mammalian Exposure Concentration Estimates for Food Uses ........................ 20 Table 12 Mammalian Exposure Concentra tion Estimates for Non-Food Uses................ 20 Table 13 Mammalian Exposure Concentration Estimates for Non-Food Uses (Roses).. 21 Table 14 Terrestrial Plant Exposure C oncentration Estimates forAcequinocyl.............22 Table 15 Acute Toxic Effects of Ac equinocyl on Freshwater Fish................................. 23 Table 16 Chronic Toxic Effects of Acequinocyl on Freshwater Fish ............................. 24 Table 17 Acute Toxic Effects of Acequinocyl on Estuarine/Marine Fish....................... 24 Table 18 Acute Toxic Effects of Acequi nocyl on Freshwater Invertebrates................... 25 Table 19 Chronic Toxic Effects of Ace quinocyl on Freshwater Invertebrates ............... 25 Table 20 Acute Toxic Effects of Acequinocyl on Estuarine/Marine Invertebrates....... 26 Table 21 Acute Toxic Effects of Acequinocyl on Aquatic Plants................................... 26 Table 22 Acute Toxic Effects of Acequinocyl on Birds.................................................. 27 Table 23 Chronic Toxic Effect s of Acequinocyl on Birds .............................................. 28 Table 24 Chronic Toxic Effects of Acequinocyl on Mammals ....................................... 28 Table 25 Developmental and Reproductive Effects of Acequinocyl on Mammals....... 29 Table 26 Effects of Acequinoc yl on Terrestrial Plants.................................................... 29 Table 27 Effects of Acequinoc yl on Non-Target Insects................................................. 30 Table 28 Effects of Acequi nocyl on Earthworms.......................................................... 31 Table 29 Summary of Aquatic Ac ute RQs Using Model Approach 1.......................32 Table 30 Summary of Aquatic Ac ute RQs Using Model Approach 2.......................32 Table 31 Summary of Aquatic Chronic RQs Using Model Approaches 1 & 2 ...........33 Table 32 Summary of Avian RQs fo r Food Uses and Non-Food Uses..................... 34 Table 33 Summary of Mammalian RQs for Food Uses........................... .......... .35 Table 34 Summary of Mammalian RQs for Non-Food Uses... ................... .........35 Table 35 Summary of Mammalian RQs for Non-Food Uses........................ .........36 Table 36 Chance of Individual Effect s for Aquatic Invertebrates.............. ...............38 Table 37 Distribution of E ndangered Species by State.........................................41 Table 38 Summary of Potential Direct and In direct Effects to Fede rally-Listed Taxa....42

List of Figures Figure 1 Chemical Structure of Acequi nocyl and its Primary Degradate .......................... 8 Figure 2 Conceptual Model for Acequinocyl .................................................................. 12

## 5 

# 1. Executive Summary The manufacturer of acequinocyl (PC 006329), Arysta LifeScience North America Corporation, submitted an application to the Environmental Protection Agency to amend the registration (EPA Reg. # 66330-38) for Kanemite 15 SC (trade name for acequinocyl). The proposed label changes include addition of uses on grap es (DP 337381), extension of the listing of crops for tree nuts, pome fruits, and citrus fru it groups (DP 330467), and expansi on of non-food outdoor uses to include forest nurseries, arboretums, public pa rks, rights-of-way, a nd recreation areas (DP 330471). Currently acequinocyl is registered fo r use in only almonds, pistachios, oranges, grapefruits, lemons, apples, pears, strawberri es, and nursery plantings. This ecological risk assessment, therefore, evaluates the use of acequi nocyl for new food (grapes, pome fruits, citrus, and tree nuts) and non-food outdoor uses (a broad use category th at includes various ornamentals). Based on the propos ed addition/amendment to the existing label for Kanemite 15 SC, the model parameters included a yearly maxi mum rate of 0.6 lb ai/A via foliar spray for both food and non-food uses (except roses).

Data and information presented in the initial risk assessm ent (DP 285811, DP 286428, DP 289153, DP 290010, DP 287269, DP 290009, and DP 291174) se rved as the backbone to this risk assessment. Five new studies [two each on daphnia (DP 309566) and bobwhite quail (DP 327741) and one on common carp (DP 309568)] were s ubmitted by the registrant subsequent to the conditional registration of Kanemite to fill th e data gaps identified in the initial risk assessment. For the purpose of this assessment, these new studies were used in addition to the studies submitted for initial registration. The new st udies can be identified by their 850 guideline series.



# 1.1 Nature of Stressor Acequinocyl, the active ingredient of the insecticide/miticide Kanemite 15 SC, is used for the control of two-spot spider mite ( Tetranychus urticae ), Pacific mite ( Tetranychus pacificus ) and Willamette mite ( Eutetranychus willamettei ) on grapes. It also controls European red mite ( Panonychus ulmi ), spruce spider mite ( Oligonychus ununquis ), and citrus red mite ( Panonychus citric ) on ornamentals and ornamental nur series; two-spot spider mite ( Tetranychus urticae ), Pacific mite ( Tetranychus pacificus ), and European red mite ( Panonychus ulmi ) on tree nuts and pome fruits; citrus red mite ( Panonychus citric ) and Texas citrus mite ( Eutetranychus banski ) on citrus; and strawberry spider mite ( Tetranychus turkestani ) and two-spot spider mite ( Tetranychus urticae ) on strawberries.

Both the parent acequinocyl (3-dodecyl -1,4-dihydro-1,4-dioxo-2-naphthyl acetate) and its primary degradate, 2-dodecyl-3-hydroxy-1,4-napthoqui none (also referred to as R1, deacetylated acequinocyl, acequinocyl-OH, OH-A KD 2023, or AKM-05) appear to mediate the chemical’s mode of action through the inhibition of electr on transfer in mitochondria. Acequinocyl is classified as very highly toxic to estuarine/marine invertebrates, slightly to highly toxic to estuarine/marine fish, non-toxic to moderately toxic to birds, and non-toxic to honey bees.

## 6 

# 1.2 Potential Risk The screening-level risk assessment for acequino cyl identified potential concerns for direct effects on aquatic invertebrates, birds, and ma mmals. Details on potenti al risk to individual organisms in aquatic and terrestrial environments are discussed below.

Aquatic Organisms Among the aquatic organisms tested, invertebrate s (both in freshwater and estuarine/marine media) are highly sensitive to acequinocyl. Analys is suggests that the pr oposed acequinocyl uses on grapes, pome fruits, citrus, tree nuts, and or namentals pose acute and chronic risk to both endangered and non-endangered freshwater and estu arine/marine invertebrates. On the other hand, no risk concerns exist for acute or chroni c direct effects to ei ther endangered or nonendangered fish in freshwater or saltwater environments.

Only one freshwater non-vascular plant, Pseudokichneriella subcapitata , was evaluated to test the acute effects of acequinocyl. Calculated risk quotient for this species was lower than the agency-established endangered risk LOC for aqua tic plants suggesting that proposed acequinocyl uses do not pose any risk to aquatic plants.

Terrestrial Organisms Acute RQs were not calculated for birds (oral and dietary) and mammals (oral) because there were no definitive acute toxicity values. Because there was no mortality or sublethal effects at the highest acequinocyl concentrations tested a nd as exposure concentrat ions were below these levels, no acute effects are expected.

Chronic exposure of mammals to acequinocyl do ses from foraging on shor t grass, tall grass, broadleaf plants, and small insects appear to pos e risk for all the proposed uses. Chronic dietarybased RQs, on the other hand, exceeded agency’s LOC slightly (RQ = 1.07 to 1.2) for all birds and mammals that consume short grass only when acequinocyl was assumed to be stable to all routes of degradation in the environment. As it is unlikely that birds and mammals feed exclusively on shortgrass and as RQ values are only slightly greater th an agency’s LOC of 1.0, chronic (dietary) risk is to mammal s is not expected to be high.

Being an insecticide, acequinocyl do not appe ar to pose non-endangered or endangered species risk concerns for plants in bot h dry and semi-aquatic habitats.



# 1.3 Endangered Species Assessment The Locates database (Version 2.10.3) was searched to determine the endangered species present in the states where proposed acequinocyl uses wi ll occur. A total of 1144 species were found to be present in the US states where grapes, citrus fruits, pome fruits, floricultural crops, nursery crops, and tree nuts are grown in the United States . The number of endangered species in various taxa that may directly or indirectly be affected by acequinocyl applicatio ns in the proposed use states are presented in Table 1.

## 7 Table 1. Number of Endangered Species Occurring in the Same States as the Proposed Crops Taxa Number of Species Mammals 144 Birds 231 Amphibians 22

Reptiles 75

Fish 270 Crustaceans 24

Bivalves 213 Gastropods 75 Arachnids 13

Insects 77

Based on LOC exceedances, it appears that endangere d species of aquatic invertebrates, birds, and mammals will experience direct effects from the new uses of acequinocyl (Table 2). Since birds serve as surrogates to terrest rial amphibians and reptiles, these taxa are also at direct risk from the new uses of acequinocyl. Primary impact on the above taxa will be loss of species due to mortality leading to structural and functional changes of the ecosystem in which they thrive. Changes resulting from mortality will be manifested in the form of reducti on in species richness, flux in community composition, an d disruption of food chain.

Table 1. Summary of Potential Direct a nd Indirect Effects to Federally-Listed Taxa Organism Direct Effects Indirect Effects 1 

## Terrestrial and semi-aquatic monocots and dicots No Yes

Terrestrial invertebrates No Yes Birds Yes Yes Terrestrial phase amphibians Yes Yes Reptiles Yes Yes Mammals Yes Yes Aquatic vascular plants No Yes Freshwater fish No Yes Aquatic phase amphibians No Yes Estuarine/marine fish No Yes Freshwater invertebrates Yes Yes Estuarine/marine invertebrates Yes Yes

1 

### Specific indirect effects were not identifie d as refined analysis was not conducted

## 8 

# 2. Problem Formulation Problem formulation, which provid es direction and scope to a ri sk assessment, consists of defining the problem and purpose for the assessm ent, and developing a plan to analyze and characterize risk. The critical components of th e problem formulation ar e formulation of risk hypotheses and the conceptual model, selection of the assessment endpoints, and development of an analysis plan. The analysis plan and suppor ting rationale determine whether the uses of acequinocyl, as an insecticide, c ould result in exposures that ca use unreasonable adverse effects (risk) to non-target organisms including those fede rally listed as threatened or endangered.



# 2.1 Stressor Description Acequinocyl belongs to the quinone class of chem icals. Its physical and chemical properties are listed below:

CAS Registry Number: 57960-19-7 Appearance: yellowish crystalline powder Empirical Formula: C 24 

## H 32 

## O 4 

## Molecular Weight 384.5 Vapor Pressure: 1.69 x 10 -6 

## Pa at 25 ° 

## C (MRID 45434905) Henry’s Law Constant: 3.9 x 10 -5 

## (MRID No. 45434905) Water solubility: 6.7 μ g/L in water (MRID 45434906) Solvent solubility: 44 g/L (n-hexan e); 620 g/l (dichloromethane); 7.8 g/l (methanol); n-octanol (3 1 g/L); 220 g/L (acetone) Melting point: 59.6 ° 

## C Partition coefficient: >6.2 at 25 ° 

## C (log Pow)

One of the major metabolites of acequinocyl, hydroxy acequinocyl or R1, in addition to the parent, is capable of inhibiting th e electron transfer in mitochondria. It is not clear at this point what proportion of the miticidal action is throug h the parent or the metabolite R1 or both. Structures of acequinocyl and its primar y degradate R1 are shown in Figure 1.



# Figure 1: Chemical Structures of Aceq uinocyl and its Primary Degradate

O O

CH 2 

## (CH 2 

## ) 10 

## CH 3



## OCOCH 3 

## O O

CH 2 

## (CH 2 

## ) 10 

## CH 3



## OH



# Acequinocyl Hydroxy acequinocyl or R1

2.2 Environmental Fate Summary Acequinocyl appears to undergo fairly rapid transformation in most aquatic and terrestrial

## 9 environments. Based on the environmental fate data reviewed for the initial registration, acequinocyl undergoes hydrolysis with a half-life of a few hours to less than 2 days under neutral and alkaline pH conditions (MRID 45434908). However, it is stable at acidic pH conditions. Photodegradation in water appears to be the main route of degradation in aquatic environments, with half-lives occurring in less than 15 minutes in sterile lab a nd river water. Acequinocyl also undergoes fast degradation in terrestrial environments. The half-lives for aerobic biotransformation on soil are less than 2 da ys under laboratory conditions (MRID 45434911). Under field conditions, acequinocyl applied to bare plots dissipated with half-lives ranging from 2 hrs in California to 14 hours in New York (MRID 45651601). In contrast, the residue decline data submitted for the Magnitude of Residue stud ies on apples, pears, oranges, grapefruit, lemon and almonds indicated a mean half-life of 20 days.

Acequinocyl is expected to exhibit low mobility in soil. The soil adsorption K d 

## for acequinocyl ranges from 678 ml/g in sandy loam soil to 1,620 ml/g in silty clay loam soil (MRID 45531902). In field studies, the parent was not detected below 0-15 cm soil depth (MRID 45651601). The major transformation products detected in th e field include R1 (2-dodecyl-3-hydroxy-1, 4- napthoquinone) and AKM-18 [2-(1,2-di oxotetradecyl) benzoic acid].

The bio-concentration factor for acequinocyl in fish ranged from 307 to 387 (MRID 45434923). Therefore, it is expected to have a moderate tend ency to accumulate in fish. More details on fate studies and other fate properties of acequinocyl can be accessed from the original risk assessment document.



# 2.3 Mode of Action Acequinocyl is primarily applied as a foliar sp ray. It has no systemic activity but provides contact control of all life stages of mites. Therefore, thorough plant coverage is the key for effective mite control. To a lesser extent, a cequinocyl is also effective through ingestion.

Acequinocyl’s mode of action is through mitochondr ial electron transport inhibition. Upon application, acequinocyl hydrolyses to hydroxy ace quinocyl (R1) in the mite body and binds to the Qo center at complex III in mitochondria. The net result is the blockage of cellular respiration and other adverse metabolic effects in mites. Both the parent acequinocyl and its major metabolite (R1) are thought to be capable of inhibiting the elec tron transfer in the mitochondria. It is not clear at this point what po rtion of the miticidal action is contributed by the parent, metabolite R1, or both.



# 2.4 Use Characterization The proposed label addition/amendment for acequinoc yl includes its use in grapes, pome fruits, citrus, tree nuts, and outdoor applications. The outdoor uses are primarily non-food and include applications to ornamentals in arboretums, greenhouses, industrial and institutional areas, interiorscapes, nurseries, landscapes, recreati onal sites, residences, rights-of-way, and shadehouses.

## 10 The recommended maximum application rate for aceq uinocyl in food crops (grapes, pome fruits, citrus, and tree nuts) is 0.3 lb s ai/A, applied as a foliar spra y twice per crop year, with a minimum interval between applications of 21 days . The label states that the maximum rate per application and per year for ornamentals (o ther than roses) is 0.125 and 0.6 lbs ai/A, respectively. Based on the above, it is assumed that a maximum of five acequinocyl applications can be made to ornamentals per year. Based on th e initial risk assessment, the minimum interval between applications for ornamentals is assume d to be 14 days. The labeled maximum rate per application and annually for roses is 0.06 and 0.31 lb ai/A, respectively.

For both food as well as non-food uses, the label specifies that the pr oduct be applied by ground and not by air. The label also mentions that acequinocyl should not be applied through any irrigation system and within 75 feet of aquatic ar eas. For the purpose of aquatic and terrestrial modeling, application inform ation on the label is interpreted as follows:

• Application method: foliar spray

• Maximum application rate: 0.3 lb ai/A (food uses); 0.125 lb ai/A (non-food uses except roses); 0.06 lb ai/A (non-food uses – roses)

• Maximum # of applications: 2/crop year (food uses); 5/crop year (all non-food uses including roses)

• Application interval: 21 days (f ood uses); 14 days (all non-food uses)

• Maximum yearly rate: 0.6 lb ai/A ( both food and non-food uses except roses) 0.31 lb ai/A (non-food uses - roses)

The geographic distribution of crops for wh ich new acequinocyl uses are proposed are summarized below:



# Citrus Fruits The majority of U.S. citrus production is concentr ated in Florida, California, Arizona, and Texas and includes oranges, grapefruit, lemons, tangelos, tangerines, a nd temples. In 2006, citrus fruits were harvested from 0.88 million acres in the United States (USDA-NASS).



# Grapes About 14 US states (Arizona, Arkansas, Califor nia, Georgia, Michiga n, Missouri, New York, North Carolina, Ohio, Oregon, Pennsylvania, Texa s, Virginia, and Washington) planted grapes on 937,200 acres in 2006 (USDA-NASS). California ra nked number one in the production of all grape types (table, wine, and ra isin) by planting 85% of the tota l US grape acreage. Other major grape producing states, in the order of production, include Washington, New York, Michigan, Oregon, and Pennsylvania.



# Pome Fruits Pome fruits are all members of rose family and in clude fruits such as apples, pears, quince, and loquats. Apples and pears, the most known in this fruit group, were planted on 377,490 and 59,850 acres, respectively, in 2006 (USDA-NAS S). Washington, New York, Michigan,

## 11 California, and Pennsylvania are the major apple producing states in the United Sates contributing about 42, 12, 11, 6, and 6%, respecti vely, to the total pl anted acreage in 2006. Washington is the number one pear-producing state followed by Oregon and California. 

# Tree Nuts Tree nuts are all perennial crops and need high initial capital investment and a long wait for return realization. The US tree nut industry comprises of nuts that grow on trees such as almonds, pistachios, pecans, hazelnuts, macadamias, and walnuts. In 2006 the bearing acreage of these crops was 951,100 acres. Almonds alone acc ounted for 61% of the total acreage and production value derived from the tree nuts (USDA-NASS).



# Ornamentals With over 2000 different crops grown commercia lly, ornamentals includ e floriculture and nursery crops, which are the two fastest grow ing major segments of U.S. agriculture. The floriculture industry comprises of a group of cr ops without woody stems that are sold for their flowers as annual and perennial bedding/garden pl ants, potted plants, cut flowers, cut foliage/cut greens, outdoor foliage, and propaga tive materials. Nursery crops ar e ornamental plants and trees with woody stems that include broadleaf evergr eens, coniferous evergreens, deciduous shade trees, deciduous flowering trees, deciduous shrubs and other orname ntals, fruit and nut plants intended for outdoor and landscape us e, cut and live Christmas tree s, propagation material or lining-out stock, ornamental vines, tu rf grass sod and other groundcovers.



# 2.5 Assessment Endpoints Assessment endpoints aid in evaluating potential risk to an ecosys tem from pesticide applications. End points for a part icular pesticide are determined based on toxicity information from guideline tests and open lite rature data. Endpoints are measur ed at the individual level to provide insight about risks at hi gher levels of biological orga nization such as populations and communities.

In screening-level risk assessment, endpoints for both aquatic and terrestrial animal species are survival, growth, and reproductive impairment. For terrestrial and semi-aquatic plants, the screening assessment endpoint is plant perpet uation (measured as seedling emergence and vegetative vigor). For aquatic pl ants, the assessment endpoint is growth rate and/or biomass production.



# 2.5.1 Ecosystems Potentially at Risk The ecosystem primarily at risk from acequinocyl a pplications is the crop field and outdoor area to which it is applied. Biota, fauna more than flora, in crop fields , outdoor areas, and areas adjoining the sprayed en vironment, may be affected due to acequinocyl application and accumulation. Terrestrial species of potential co ncern in the treated areas include non-target insects, amphibians, reptiles, birds, and ma mmals. The adjacent aquatic and terrestrial environments may also be affected due to runoff or spray drift or both. Aq uatic species at risk from acequinocyl runoff and drift include freshwater fish and invertebrates, estuarine/marine fish and invertebrates, and amphibians.

## 12 Being an insecticide, acequinocyl may not adversel y impact plants in the terrestrial or aquatic ecosystems. However, based on the potential to bind to soil with K d 

## s ranging from 678 to 1,620 ml/g, acequinocyl may cause adverse effect s on sediment-dwelling organisms. The bioaccumulation factor (BCF of 307-387) for ace quinocyl further indica tes its tendency (though moderate) to accumulate in fish.

The conceptual model, presented in Figure 2, generically depicts th e potential source of acequinocyl, release mechanisms, abiotic receiv ing media, biological receptor types, and possible direct and indirect effects due to exposure to acequinocyl. Acequi nocyl is expected to exhibit low mobility in soil and lo w potential to leach to ground water due to its high Koc values. Therefore, exposure estimates fr om leaching to groundwater were not evaluated in this risk assessment. Potential emission of volatile compo unds is not considered as a viable release mechanism for acequinocyl because of a borderline Henry’s Constant (3.9 x 10 -5 

## ).



# Figure 2. Conceptual Mo del for Acequinocyl

1 

### Exposure pathways in dotted boxes are not evaluated in view of acequinocyl’s low vapor pressure (1.69 x 10 -6 

### Pa 25 0 

### C) and low soil mobility (K oc 

### = 77,400 ml/g) Food Web Dynamics Perturbing Forage or Prey Availability Habitat Alteration: impacting nesting ability, ability to seek cover, etc. Individual Terrestrial Animals Reduced Survival Reduced Growth Reduced Reproduction Individual Terrestrial Plants Seedling Emergence Vegetative Vigor Individual Aquatic Vertebrates and Invertebrates Reduced Survival Reduced Growth Reduced Reproduction Aquatic Plant Population Reduced Growth Rates and Biomass Terrestrial Animals Birds, Mammals,Reptiles, Terrestrialphase Amphibians, Bees Terrestrial Upland Plants Wetland/ Riparian Plants Aquatic Invertebrates Aquatic Vertebrates Aquatic Plants Ace q uinoc y l A pp lied as Foliar S p ra y 1

Volatilization/ Wind Suspension Ground Stressor

Source/ Transport Pathway

Source/ Exposure Media

Exposure Method

Receptors

Direct Effects

Indirect Effects Runoff/ Erosion Leaching (Infiltration/ Percolation Spray Drift

p osition De )

Terrestrial Food Residues (foliage, fruit, insects)/ Soil Upland Foliage/ Soil Riparian/ Wetland Foliage/Soil Surface Water, Sediment Ground

water

Dietary

u Direct Ingestion Direct Contact/ Root Uptake Direct Contact/ Root Uptake Gill/ Integument Uptake p take

## 13 

# 2.5.2 Ecological Effects Ecological effects for the screen ing level risk assessment are ba sed on a suite of registrantsubmitted toxicity studies performed on a limited number of organisms in the following broad groupings:

• Birds - also used as a surrogate for te rrestrial phase amphibians and reptiles

• Mammals

• Fish (freshwater and estuarine/marine) - al so used as a surrogate for aquatic phase amphibians

• Invertebrates (freshwater and estuarine/marine)

• Plants (terrestri al and aquatic)

Evaluation of ecological effects focuses initially on direct e ffects to the above groups of organisms residing in the ecosystems at risk. Dir ect effects evaluated ar e survival, growth, and reproduction of various taxa of organisms potenti ally exposed to acequinocyl. Both acute and chronic effects are considered.

The Risk Quotient method is the approach used by EFED to estimate the risk to aquatic and terrestrial species (Appe ndix F). This method involves integrat ing the results of exposure and ecotoxicity data. Risk quotients (RQs) are ca lculated by dividing the exposure estimates by the acute or chronic toxicity values (endpoints). If pre- established levels of concern (LOCs) are exceeded for direct effects, indirect effects to endangered species ( e.g ., changes in food chain and decrease in community diversity) are eval uated based on the group of organisms exceeding the LOC.



# 2.6 Risk Hypotheses

• Terrestrial vertebrates (birds, mammals, rept iles, and amphibians) are subject to adverse direct effects such as reduced survival , growth, and reproduction when exposed to acequinocyl/hydroxy acequinocyl due to th e labeled use of acequinocyl.

• Aquatic invertebrates, fish, and amphibians in surface waters (fresh water or saltwater) receiving spray drift or runoff from treated fields following acequinocyl application are subject to adverse effects such as reduced growth, survival, and reproduction when exposed to acequinocyl/hydr oxy acequinocyl residues.

• Aquatic plants may be affected advers ely when exposed to acequinocyl/hydroxy acequinocyl either through contac t, sorption, or root uptake.

• Non-target terrestrial plants may be subject to adverse effects such as reduction in vegetative vigor and seedling emergen ce when exposed to acequinocyl/hydroxy acequinocyl.



# 2.7 Key Uncertainties and Information Gaps A major source of uncertainty, both in the initial and the current aquatic risk assessment, is due to 2 common issues identified in most of the su bmitted fate studies. First, the analytical methods used in the fate studies were inappropriate to accurately assess the ra te of degradation of acequinocyl and to identify its major transfor mation products. The second common deficiency is

## 14 the lack of storage stability data to determine if the degradation of [ 14 

## C] acequinocyl and its transformation products occurred during st orage and before sample analysis.

The current risk assessment is also limited due to the lack of studies on certain key species. For example, acequinocyl is classified as very highly toxic to aqua tic organisms and yet no studies were submitted to document the chronic effects of acequinocyl on estuarine/marine invertebrates. The above was identified as a data gap in the initial risk assessment also. EFED requested a mysid life cycle study as part of the conditiona l registration granted fo r acequinocyl in 2003. As a follow-up to the conditional registration, the re gistrant submitted a study waiver request in 2006 citing that instability and poor recovery of acequinocyl in saline water posed problems in determining chronic effects of acequinocyl on mysid shrimp. In light of this data gap and due to greater sensitivity of estuarine/marine invert ebrates to acequinocyl compared to freshwater invertebrates, the current assessment assumes that acequinocyl poses chronic risk to estuarine/marine invertebrates, similar to freshwater invertebrates.

Uncertainty also stemmed from the selection of toxicity endpoints from the studies that used formulated product (instead of the technical grade active ingr edient as suggested in the guidelines) (example: acute eff ects of acequinocyl on estuarine/marine fish; chronic effects of acequinocyl on freshwater fish). Th is is due to the poor recovery of the technical grade product compared to the formulated product. Risk may have been under/overestimated in these situations when the end point was derived from studies that used formulated product. Furthermore, none of the fish and bird studies reported a definitive end point on acute effects as a result of which uncertainty increased in the risk assessment.

In view of the lack of monitoring data for aceq uinocyl, this risk assessment is based on model predicted estimated environmental concentrations (EECs). Crops chosen for modeling represent a wide geographic area encompassing a variet y of environmental conditions. The PRZM/ EXAMS scenarios used in this assessment pr ovide reasonable coverage of acequinocyl use pattern and use areas. The lack of additional modeling scenarios may have added uncertainty to the assessment. 

# 3. Exposure Assessment

3.1 Label Application Rates and Intervals Acequinocyl use rate per app lication, application frequency, maximum yearly rate, and time interval between applications fo r both food and non-food uses are de tailed below in Table 3. This information is obtained from the label and is used in the exposure assessments. Acequinocyl application dates were derived from USDA’s Crop Profiles and EFED’s Crop Scenarios.

Since the application rate, numbe r of applications, and the interval between applications are same for grapes, pome fruits, tree nuts, and ci trus, the EECs are grouped under one category as food crops. The application rate for ornamentals (non-food uses except roses) is 58% lower than food crops (0.125 lbs ai/A for ornamentals versus 0.3 lbs ai/A for food crops); however the yearly maximum rates of application are same for both uses. Acequinocyl application rate for roses is 52% lower than the other ornamentals.

## 15 Table 3. Acequinocyl Application Information

Type of

Use Rate/ Application

(lb ai/A) Maximum Application Rate/Year

(lb ai/A) Maximum Number of Applications/ Year Application Interval

(Days) Application Date for Various Scenarios

Food 0.3 0.6 2 21 15 June - CA almonds 1 July – GA pecans 15 March – FL citrus 15 May – CA citrus 15 April – CA grapes 15 July – NY grapes 1 July – OR, PA, and NC apples Non-Food

Non-Food

(roses) 0.125

0.06 0.6

0.30 5

5 14

14 1 July – OR Christmas

trees 15 March – FL nursery plants 15 March – FL nursery plants



# 3.2 Aquatic Exposure Estimates Due to the uncertainties associated with the analy tical methods used in fate studies, two different approaches were used for the aquatic exposure modeling in the current assessment, similar to that used in the initial assessment. Assumptions made for the 2 approaches are presented in Table 4. In the first approach (Model Approach 1), th e parent was assumed to be stable in the environment to address the uncertainty associated with the half-lives calculated for acequinocyl from fate studies. In the second approach (Model Approach 2), the half-lives calculated for the parent and the major degradate R1 were assu med valid and the combined residues were estimated in the surface and ground water to address the toxicity concerns associated with R1 degradate.

Table 4 Assumptions for Model Approaches 1 and 2 Parameter Model Approach 1 

## Model Approach 2 

## Source

K d 

### 678 ml/g 27 ml/g MRID # 45531902; 45434907 Photolysis Half-life stable 0.19 days MRID # 45434909

Aerobic Aquatic

Metabolism stable 97 days twice the aerobic soil metabolism t 1/2 

### based on the Input Parameter Guidance Manual,

February, 2002 1 

### Anaerobic Aquatic Metabolism stable 649.8 days MRID # 45434913

Aerobic Soil Metabolism stable 48.5 days MRID # 45434911 Hydrolysis stable 9.6 days MRID # 45434908

1 

### Parent was assumed to be stable in the environment; 2 

### Combined values for parent and R1

## 16 

# 3.2.1 PRZM-EXAMS Modeling Inputs and Scenario Selection EFED’s available standard crop scenarios were us ed to evaluate the aquatic exposure resulting from acequinocyl applications to grapes, citrus, tree nuts, pome fr uits, and ornamentals. Standard scenarios were selected for each of these crop groups to assess runoff potential from vulnerable use sites

. Application rates varied base d on type of use as shown in Tables 3 and 5. Other input parameters were selected according to EF ED Input Parameter Guidance for PRZM/EXAMS 1



## . Input parameters ar e shown in Table 5.

Table 5. Input Parameters for PRZM-EXAMS Modeling Parameter Value Comments

Application Rate 0.34 kg ai/ha for food crops (citrus, grapes, tree nuts, and pome fruits) 0.140 kg ai/ha for non-food crops (except roses) Product Label

Maximum Number of Applications per Year 2 applications for food crops 5 applications for non-food crops (except roses) Product Label

Interval between applications 21 days for food crops 14 days for non-food crops Product Label

Application Efficiency 0.99 -

Molecular Weight 384.5 Product Chemistry

Water Solubility 0.067 mg/l Product Chemistry

Henry’s Law Constant 3.9 e-5 atm m 3 

### /mol Product Chemistry



# 3.2.2 PRZM-EXAMS Modeling Output Using the scenarios detailed in Table 3, the Ti er II PRZM/EXAMS model estimated the aquatic concentrations for acequinocyl resulting fr om both food and non-food uses employing both model approaches (Appendix A; Tables 6 and 7). These estimated environmental concentrations (EECs) were used to calculate risk to aquatic animals and plants.

The predicted aquatic acequinocyl concentrations are lower with model approach 2 compared to model approach 1. Since approach 1 modeled parent concentrations only it is logical to expect that the concentrations predicte d in model 1 are lower than mode l 2 which estimated both parent and metabolite R1 concentrations. However, the modeled surface water concentrations are slightly conservative in the first approach compar ed to the second due to the use of stable halflives for environmental degradation in model 1 (i .e., input value is zero for biotic and abiotic degradation).

The 1-in-10-year peak concentrations for ace quinocyl ranged from 0.31 (CA citrus) to 6.57 ppb (PA apples) with model approach 1 and 0.18 (CA ci trus) to 5.57 (FL nursery plants except roses)

1 

### Guidance for Selecting Input Parameters in Modeling the Environmental Fate and Transport of Pesticides. Version II, 2/28/02

## 17 ppb with model approach 2. The 60-day average concentrations for acequinocyl ranged from 0.10 to 1.84 and 0.04 to 1.26 ppb with model approaches 1 and 2, respectively.

Table 6. Estimated Environmental Concentr ations (EEC) Based on PRZM-EXAMS and Model Approach 1 Peak 21 day Average 60 day Average Crop Scenario

EEC ( μ g/L or ppb) Food Uses CA Grapes 1.58 0.39 0.32 NY Grapes 5.77 1.99 1.53

FL Citrus 3.63 1.16 0.88 CA Citrus 0.31 0.11 0.10 CA Almonds 2.18 0.48 0.44

GA Pecans 5.65 1.97 1.48 OR Apples 2.27 0.81 0.59 PA Apples 6.57 2.37 1.84 NC Apples 3.76 1.12 0.92 Non-Food Uses OR Christmas Trees 0.89 0.27 0.19

FL Nursery Plants 4.82 1.47 1.28 FL Nursery Plants (Roses) 2.41 0.73 0.64

Table 7. Estimated Environmental Concentr ations (EEC) Based on PRZM-EXAMS and Model Approach 2

Peak 21 day Average 60 day Average Crop Scenario

EEC ( μ g/L or ppb) Food Uses CA Grapes 0.21 0.07 0.05 NY Grapes 2.78 1.01 0.53

FL Citrus 1.99 0.62 0.29 CA Citrus 0.18 0.06 0.04 CA Almonds 0.64 0.22 0.10

GA Pecans 5.05 1.78 0.86 OR Apples 0.81 0.27 0.15 PA Apples 3.59 1.36 0.70 NC Apples 3.03 1.09 0.57 Non-Food Uses OR Christmas Trees 0.33 0.11 0.06

FL Nursery Plants 5.57 2.39 1.26 FL Nursery Plants (Roses) 2.79 1.19 0.63

## 18 In general, aquatic concentrations reported in the current risk assessment are greater than those reported in the initial risk assessment. This is due to changes made to acequinocyl application timing for the crop scenarios. Furthermore, addi tion of new crop scenarios in the current risk assessment may have resulted in different results than the initial assessment.



# 3.3 Terrestrial Exposure Estimates Acequinocyl exposure concentratio ns for terrestrial fauna were determined using the screeninglevel exposure model, TREX (Version 1.3.1) (US EPA 2006). TREX simulation model incorporates the nomogram (Fletcher et al., 1994 ; Hoerger and Kenaga, 1972; Pfleeger et al., 1996) relationship between the amount of pesticide applied and the amount of pesticide residue present on a given food item. TREX calculates pesticide residu es on each food item on a daily interval for one year using a first order decay function based on the concentrations present from both the initial and add itional applications.

Estimated Environmental Concentrations were simulated using the default foliar dissipation halflife of 35 days as well with chemical specific half-life determined fr om the Magnitude of Residue Studies (OPPTS 860.1500). In the initial risk assessment, a first-or der foliar dissipation half-life was estimated for apples (t 1/2 

## = 20.5 days), pears (t 1/2 

## = 7.63 days), oranges (t 1/2 

## = 16.1 days) and almonds (t 1/2 

## = 17.9 days) using the acceptable Ma gnitude of Residue Studies for acequinocyl. Based on the half-lives determin ed for these four f ood items, the upper 90% confidence bound on the mean half-life of 20 days was derived and used in calculating the residue concentrations in additi on to the default half-life of 35 days. Input parameters used in TREX model are presented in Appendix B. Out puts generated by TREX are in Appendix C.



# 3.3.1 Birds and Mammals Exposure concentration estimates are presented in Tables 8, 9 and 10 for birds and Tables 11, 12, and 13 for mammals. These estimates are based on the upper Kenaga bound dose and the assumption that the species in que stion eats one type of food item and forage only in the treated and/or overspray areas.

Use of acequinocyl on food crops generated the most conservative estimate for exposure concentrations. Even though the yearly maxi mum use rates were same for both food and nonfood uses (except roses), exposure concentratio ns in general, were greater for food uses compared to non-food uses (except rose s) with either half-life periods.

Both dietary and dose-based exposure concentr ations for birds and mammals were 11, 23, and 23% lower when acequinocyl used for food, non- food uses (except roses), and non-food uses (roses), respectively, degraded to half its origin al concentration in 35 days compared with 20 days. Avian exposure (oral) doses for food us es ranged from 2.17 to 136.1 ppm and 1.94 to 121.6 ppm with 35 and 20 day half-lives, respectively. Mammalian doses (oral) for food uses ranged from 0.25 to 113.94 ppm and 0.23 to 101.80 ppm with 35 and 20 day half-lives, respectively.

## 19 Table 8. Avian Exposure Concentration Estimates for Food Uses Dose-based EECs (mg/kg-bw) Feeding Category Dietary-basedEECs (mg/kgfood item) Small (20 g) Medium (100 g) Large (1000 g) Acequinocyl half-life 35 days ; application twice at 0.30 lb ai/A 21 days apart Short grass 119.50 136.10 77.61 34.75 Tall grass 54.77 62.38 35.57 15.93 Broadleaf plants/small insects 67.22 76.56 43.66 19.55 Fruits/pods/seeds/large insects 7.47 8.51 4.85 2.17 Acequinocyl half-life 20 days ; application twice at 0.30 lb ai/A 21 days apart Short grass 106.77 121.60 69.34 31.05 Tall grass 48.94 55.74 31.78 14.23 Broadleaf plants/small insects 60.06 68.40 39.01 17.46 Fruits/pods/seeds/large insects 6.67 7.60 4.33 1.94



## Table 9. Avian Exposure Concentration Estimates for Non-Food Uses

Dose-based EECs (mg/kg-bw) Feeding Category Dietary-basedEECs (mg/kgfood item) Small (20 g) Medium (100 g) Large (1000 g) Acequinocyl half-life 35 days; five applications each at 0.125 lb ai/A 14 days apart Short grass 92.92 105.83 60.35 27.02 Tall grass 42.59 48.50 27.66 12.38 Broadleaf plants/small insects 52.27 59.53 33.95 15.20 Fruits/pods/seeds/large insects 5.81 6.61 3.77 1.69 Acequinocyl half-life 20 days; five applications each at 0.125 lb ai/A 14 days apart Short grass 71.14 81.02 46.20 20.69 Tall grass 32.61 37.13 21.18 9.48 Broadleaf plants/small insects 40.02 45.57 25.99 11.64 Fruits/pods/seeds/large insects 4.45 5.06 2.89 1.29



## Table 10. Avian Exposure Concentration Estimates for Non-Food Uses (Roses)

Dose-based EECs (mg/kg-bw) Feeding Category Dietary-basedEECs (mg/kgfood item) Small (20 g) Medium (100 g) Large (1000 g) Acequinocyl half-life 35 days; five applications each at 0.06 lb ai/A 14 days apart Short grass 44.60 50.80 28.97 12.97 Tall grass 20.44 23.28 13.28 5.94 Broadleaf plants/small insects 25.09 28.57 16.29 7.29 Fruits/pods/seeds/large insects 2.79 3.17 1.81 0.81 Acequinocyl half-life 20 days; five applications each at 0.06 lb ai/A 14 days apart Short grass 34.15 38.89 22.18 9.93 Tall grass 15.65 17.82 10.16 4.55 Broadleaf plants/small insects 19.21 21.88 12.47 5.59 Fruits/pods/seeds/large insects 2.13 2.43 1.39 0.62

## 20 Table 11. Mammalian Exposure Concentration Estimates for Food Uses Dose-based EECs (mg/kg-bw) Feeding Category Dietary-basedEECs (mg/kgfood item) Small (15 g) Medium (35 g) Large (1000 g) Acequinocyl half-life 35 days ; application twice at 0.30 lb ai/A 21 days apart Herbivores and Insectivores Short grass 119.50 113.94 78.75 18.26 Tall grass 54.77 52.22 36.09 8.37 Broadleaf plants/small insects 67.22 64.09 44.29 10.27 Fruits/pods/seeds/large insects 7.47 7.12 4.92 1.14 Granivores Fruits/pods/seeds/large insects - 1.58 1.09 0.25 Acequinocyl half-life 20 days ; application twice at 0.30 lb ai/A 21 days apart Herbivores and Insectivores Short grass 106.77 101.80 70.36 16.31 Tall grass 48.94 46.66 32.25 7.48 Broadleaf plants/small insects 60.06 57.26 39.58 9.18 Fruits/pods/seeds/large insects 6.67 6.36 4.40 1.02 Granivores Fruits/pods/seeds/large insects - 1.41 0.98 0.23



## Table 12. Mammalian Exposure Concentration Estimates for Non-Food Uses Dose-based EECs (mg/kg-bw) Feeding Category Dietary-basedEECs (mg/kgfood item) Small (15 g) Medium (35 g) Large (1000 g) Acequinocyl half-life 35 days ; application twice at 0.125 lb ai/A 14 days apart Herbivores and Insectivores Short grass 92.92 88.59 61.23 14.20 Tall grass 42.59 40.61 28.06 6.51 Broadleaf plants/small insects 52.27 49.83 34.44 7.99 Fruits/pods/seeds/large insects 5.81 5.54 3.83 0.89 Granivores Fruits/pods/seeds/large insects - 1.23 0.85 0.20 Acequinocyl half-life 20 days ; application twice at 0.125 lb ai/A 14 days apart Herbivores and Insectivores Short grass 71.14 67.83 46.88 10.87 Tall grass 32.61 31.09 21.49 4.98 Broadleaf plants/small insects 40.02 38.15 26.37 6.11 Fruits/pods/seeds/large insects 4.45 4.24 2.93 0.68 Granivores Fruits/pods/seeds/large insects - 0.94 0.65 0.15

## 21 Table 13. Mammalian Exposure Concentration Estimates for Non-Food Uses (Roses) Dose-based EECs (mg/kg-bw) Feeding Category Dietary-basedEECs (mg/kgfood item) Small (15 g) Medium (35 g) Large (1000 g) Acequinocyl half-life 35 days ; application twice at 0.06 lb ai/A 14 days apart Herbivores and Insectivores Short grass 44.60 42.52 29.39 6.81 Tall grass 20.44 19.49 13.47 3.12 Broadleaf plants/small insects 25.09 23.92 16.53 3.83 Fruits/pods/seeds/large insects 2.79 2.66 1.84 0.43 Granivores Fruits/pods/seeds/large insects - 0.59 0.41 0.09 Acequinocyl half-life 20 days ; application twice at 0.06 lb ai/A 14 days apart Herbivores and Insectivores Short grass 34.15 32.56 22.50 5.22 Tall grass 15.65 14.92 10.31 2.39 Broadleaf plants/small insects 19.21 18.31 12.66 2.93 Fruits/pods/seeds/large insects 2.13 2.03 1.41 0.33 Granivores Fruits/pods/seeds/large insects - 0.45 0.31 0.07



# 3.3.2 Plants The exposure of terrestrial plan ts to pesticides is evaluate d by EFED using the TERRPLANT model (version 1.2.1). This model is based on 2 scen arios, a runoff scenario and a drift scenario. The runoff scenario, characterized as "sheet runoff" (one treated acre to an adjacent acre for dry areas) and "channel runoff" (10 acres to a dist ant low-lying acre for semi-aquatic or wetland areas) is based on a pesticide's water solubility and the amount of pestic ide present on the soil surface and in its top one centimeter. Spray drift is assumed to be 1% for ground applications, 5% for aerial, airblast, forced air, and spray chemigation applications, and 0% for granular applications. Plant exposure concen trations are derived from a si ngle, maximum application rate only and do not allow the degradation of pestic ides for multiple application scenarios.

Estimated exposure concentrations for terrestria l plants are derived ba sed on seedling emergence and vegetative vigor. Seedling emergence EECs are derived for adjacent dry and semi-aquatic (wetland) areas, both of which include the contam ination arising from run off and spray drift. Vegetative vigor EECs are derived from contaminati on from drift alone.

The EECs for terrestrial plants from a single a pplication of acequinocyl at the maximum label rate for both food and non-food uses are presen ted in Table 14. Sin ce the solubility of acequinocyl is below 10 ppm, the runo ff value of 1% is used as the input value in terrestrial plant modeling (Appendix D). The vegetati ve vigor drift concentrations are estimated using 1% drift for ground applications.

Acequinocyl application on food crops provided the most conser vative estimate with total loading from runoff + drift of 0.006 and 0.033 lb a i/A in upland and wetla nd areas, respectively. Exposure concentration due to dr ift was same as the runoff in upland areas as it was assumed

## 22 that 1% of the applied pesticide will r un-off and drift in an upland situation.

Table 14. Terrestrial Plant Exposure Con centration Estimates for Acequinocyl Total Loading (Runoff +Drift) (lb ai/A) Drift EEC (lb ai/A) Application Rate Upland Areas Wetland Areas All Areas 0.3 lb ai/A (food uses) 0.006 0.033 0.003 0.125 lb ai/A (non-food uses) 0.0025 0.01375 0.00125 0.06 lb ai/A (non-food uses - roses) 0.0012 0.0066 0.0006 

# 4. Ecological Effects Assessment The acute and chronic effects a pesticide may prod uce in an animal or plant (terrestrial or aquatic) are evaluated based on registrant-submitte d studies and open-literature studies from the ECOTOX database. The evaluation is further refined by incorporating the ecological effects using the Ecological Incident Information System (EIIS).

Toxicity end points, which are derived from the studies that follow agen cy guideline and Ecotox database, are evaluated for the most sensitive species and the correspond ing endpoints are used in the assessment. Taxa evaluate d include plants, birds, and mamma ls for terrestrial systems and plants, invertebrates, and vert ebrates for aquatic systems.

Bobwhite quail and/or mallard duck serve as repr esentative/test species for birds and also as surrogate species for rep tiles and terrestrial-phase amphibians. Fish serve as surrogate species for aquatic-phase amphibians. Studies conducted on la boratory rats, mice, or rabbits serve as the basis for mammal data. Toxicity end points for terrestrial plants are based on a range of broadleaf and grass species. Lemna serves as te st species for aquatic plants. Where data are available, separate endpoints are used for freshwater and estuarine/marine organisms.

Studies submitted by the registrant on ecologica l effects of acequinocyl are presented in Appendix D. New studies submitted since the conditional registration can be identified by their 850 guideline series. No ECOTOX database se arch was conducted for this assessment.



# 4.1 Aquatic Effects Summary

Toxicity to Freshwater Fish Tables 15 and 16 summarize the most sensitive ac ute and chronic toxicity endpoints for fresh water fish, respectively. In view of the stability and solubility issues related to technical grade acequinocyl, formulated product was used in several fish and othe r studies. Using the formulated product, acequinocyl was found to be more stable and soluble with acceptable chemical recoveries under experimental cond itions. None of the acute freshwat er fish toxicity studies that evaluated the technical grade active ingredient established a definitive end point.

## 23 Table 15. Acute Toxic Effects of Acequinocyl on Freshwater Fish

Species Product Tested Toxicity Endpoint Toxicity Classification Study Classification MRID #

Bluegill Sunfish (Lepomis macrochirus) Technical grade LC 50 

### 96 hr: >1.1 mg ai/L NOAEC : 1.1 mg ai /L LOAEC: >1.1 mg ai/L End points affected: None Moderately toxic Supplemental 45434918

Zebrafish (Danio rerio) Technical grade LC 50 

### 96 hr: >1.1 mg ai/L NOAEC : 1.1 mg ai /L LOAEC: >1.1mg ai/L Endpoints affected: None Moderately toxic Supplemental 45434919

Common Carp

( Cyprinus

carpio ) Technical grade LC 50 

### 96 hr: >2.3 mg ai/L NOAEC : 2.3 mg ai /L LOAEC: >2.3 mg ai/L Endpoints affected: None Moderately toxic Supplemental 46370502

Rainbow Trout (Oncorhynchus mykiss) Technical grade LC 50 

### 96 hr : > 33 mg ai /L NOAEC : 33 mg ai /L LOAEC: >33 mg ai/L Endpoints affected: None Slightly toxic Supplemental 45434920 and 45868502

Rainbow Trout (Oncorhynchus mykiss) Formulated LC 50 

### 96 hr : 67 mg a.i /L* NOAEC : <11 mg ai /L

LOAEC: 11 mg ai/L Endpoints affected: Mortality Slightly toxic Acceptable 45530601

Bluegill Sunfish (Lepomis macrochirus) Formulated LC 50 

### 96 hr: >90 mg ai /L NOAEC : 90 mg ai /L LOAEC: >90 mg ai/L Endpoints affected: None Slightly toxic Acceptable 45428605

* denotes values used to calculate RQs



## The LC 50 

## values for freshwater fish, which ranged from >1.1 ppm to >90 ppm, indicate that acequinocyl is slightly to modera tely toxic. Acequinocyl (techni cal grade) is classified as moderately toxic to bluegill s unfish (a surrogate fish for warm water species) (MRID 45434918) and zebra fish (MRID 45434919) and slightly toxic to rainbow trout (a surrogate fish for cold water species) (MRID 45434920 and MRID 45868502). When the formulated product was tested, acequinocyl was slightly toxic to both warm water (MRID 45428605) and cold water species (MRID 45530601). The definitive toxicity end point derived from the rainbow trout study, which tested the formulated produc t, was used in RQ calculations.

Several fish studies were clas sified as supplemental due to problems associated with the measurement of test chemical concentrations when technical grade pr oduct was used. A common carp study (MRID 46370502), submitted af ter the conditional registrati on was granted, was able to measure the concentration of the metabolite R1 but was unsuccessful in establishing a definitive endpoint.

## 24 Chronic exposure effects of acequinocyl on freshwater fish (rainbow trout) were also determined using the formulated product (Tab le 16). The results from this study were useful in assessing risk as the experiment included a formulation bla nk and the NOAEC/LOAEC values were expressed after correcting for the amount of active ingredient.

Table 16. Chronic Toxic Effects of Acequinocyl on Freshwater Fish

Species Product Tested Toxicity Endpoint Study Classification MRID #

Rainbow Trout (Oncorhynchus mykiss) Formulated NOAEC: 0.52 mg ai /L* LOAEC: 1.1 mg ai/L Endpoints Affected: Larvae survival Supplemental 45428607

* denotes values used to calculate RQs



## Toxicity to Estuarine/Marine Fish Acequinocyl was tested on sheepshead minnow using both technical grade and formulated products (Table 17). Both studies were unabl e to establish a definitive endpoint (MRID 45434921 and MRID 45428606). Since the experiment usin g the technical ingredient had issues in maintaining acequinocyl concen trations close to nominal, endpoi nts from the study that tested the formulated product were used.

No tests were submitted on the chronic effects of acequinocyl in estuarine/marine fish.

Table 17. Acute Toxic Effects of Ace quinocyl on Estuarine/Marine Fish

Species Product Tested Toxicity Endpoint Toxicity Classification Study Classification MRID #

Sheepshead

Minnow ( Cyprinodon variegatus ) Technical Grade LC 50 

### 96 hr : >0.19 mg ai /L NOAEC :0.19 mg ai /L LOAEC: >0.19 mg ai /L Endpoints Affected: None Highly toxic Supplemental 45434921

Sheepshead

Minnow ( Cyprinodon variegatus ) Formulated LC 50 

### 96 hr :>68 mg ai /L* NOAEC: 68 mg ai /L LOAEC: >68 mg ai/L Endpoints Affected: None Slightly toxic Acceptable 45428606

* denotes values used to calculate RQs



## Toxicity to Freshwater Invertebrates Based on the most recent daphnid study (MRID 46372101) submitted by the registrant to address data gaps in conditional registra tion, it appears that both parent and R1 degradate are toxic and that R1 may be slightly more toxic than the parent with LC 50 

## s of 2.7 and 2.4 μ g ai/L, respectively (Table 18). However, in view of the uncertain ty associated with the individual effects of acequinocyl and R1 on the overall to xicity, the most conservative LC 50, 

## 2.4 μ g ai/L, was used in

## 25 the analysis

.



## Table 18. Acute Toxic Effects of Ace quinocyl on Freshwater Invertebrates

Species Product Tested Toxicity Endpoint Toxicity Classification Study Classification MRID #

Daphnia

magna Technical Grade Parent acequinocyl LC 50 

### 48 hr: 2.7 μ g ai/L NOEAC: 0.66 μ g ai/L LOEAC: 1.9 μ g ai/L R1 degradate LC 50 

### 48 hr: 2.4 μ g ai/L* NOEAC: 0.86 μ g ai/L LOAEC: 1.7 μ g ai/L Parent acequinocyl + R1 degradate LC 50 

### 48 hr: 5.2 μ g ai/L NOEAC: 1.5 μ g ai/L LOEAC: 3.6 μ g ai/L Endpoints affected: mortality and sublethal effects Very highly toxic Acceptable 46372101

Chironomus

riparius Formulated LC 50 

### 96 hr: >86 mg ai/L NOAEC: 86 mg ai /L LOAEC: >86 mg ai/L Endpoints affected: None Slightly toxic Supplemental 45782303

* denotes values used to calculate RQs



## Table 19 below describes the chronic exposure effects of acequinocyl on daphnids. The sensitive endpoints were number of young, terminal lengt h, and dry weight of the offspring. The NOAEC/LOAEC for the parent survival was reported as 0.98/1.8 ppb (MRID 45434922).

Table 19. Chronic Toxic Effects of Ace quinocyl on Freshwater Invertebrates

Species Product Tested Toxicity Endpoint Study Classification MRID #

Daphnia

magna Technical Grade NOAEC: 0.98 μ g ai /L* LOAEC: 1.8 μ g ai/L Endpoints affected: No. of young, length and dry weight Supplemental 45434922

* denotes values used to calculate RQs



## Toxicity to Estuarine/Marine Invertebrates Acequinocyl is very highly toxic to estuarine/marine invertebrates (LC 50 

## of 0.94 ppb for mysid shrimps and EC 50 

## of 0.59 ppb for shell deposition in easte rn oysters) (Table 20). No chronic study was submitted for estuarine/marine invertebrates.

## 26 Table 20. Acute Toxic Effects of Acequino cyl on Estuarine/Marine Invertebrates

Species Product Tested Toxicity Endpoint Toxicity Classification Study Classification MRID #

Eastern Oyster ( Crassostrea

virginica ) Mollusk Shell

Deposition Technical Grade EC 50 

### 96 hr : 0.59 μ g a.i/L* NOAEC: <0.11 μ g ai/L LOAEC: 0.11 μ g ai/L

Endpoints affected:

Shell deposition Very highly toxic Supplemental 45434916

Mysid Shrimp ( Americamysis bahia ) Technical Grade LC 50 

### 96 hr : 0.94 μ g ai/L NOAEC: 0.27 μ g ai/L LOAEC: 0.50 μ g ai/L

Endpoints affected:

Sublethal effects Very highly toxic Supplemental 45434917

* denotes values used to calculate RQs



## Toxicity to Plants: Two freshwater green algae studies were submitted (MRID 45435008 using technical grade acequinocyl and MRID 45428611 using formulated ace quinocyl) (Table 21). Both studies were classified Supplemental. The study that used the formulated product was conducted for only 72 hours instead of the recommended 96 – 120 hours whereas the other study (MRID 45435008) used technical grade acequinocyl instead of the recommended formulated product.

Table 21. Acute Toxic Effects of Acequinocyl on Aquatic Plants

Species Product Tested Toxicity Endpoint Study Classification MRID #

Freshwater Algae ( Pseudokichneriella subcapitata ) Technical Grade EC 50 

### 72 hr : >78.0 mg ai /L NOAEC : ≥ 78.0 mg ai/L Endpoints affected: cell density, growth rate and biomass Supplemental 45435008

Freshwater Algae ( Pseudokichneriella subcapitata ) Formulated EC 50 

### 72 hr : 0.96 mg ai/L* NOAEC/LOAEC : 0.0017/0.0055 mg ai/L Endpoints affected: cell density Supplemental 45428611

* denotes values used to calculate RQs 

# 4.2. Terrestrial Effects Summary

Toxicity to Birds The avian toxicity data (oral a nd dietary), presented in Table 22, indicate that none of the acute studies generated a definitive e ndpoint. Toxicity values or LD 50 

## s from the acute oral gavage studies using the technical grad e acequinocyl ranged from >2000 to >2250 mg/kg-bw. Studies on sub-acute effects generated LC 50 

## s ranging from >4952 to >5970 mg/kg diet. Based on this data,

## 27 acequinocyl is classified as pr actically non-toxic to upland game bird species. Based on the toxicity end point from a test that used the formulated pr oduct, however, acequinocyl is classified as moderately toxic to birds. Endpoints derived from Bobwhite quail studies were used instead of Japanese quail as Japanese quail is not the agency-approved test species.

Table 22. Acute Toxic Effects of Acequinocyl on Birds

Species Product

Tested Toxicity Endpoint Toxicity Classification Study Classification MRID #

Japanese

Quail ( Coturnix coturnix japonica ) Technical Grade LD 50 

### 14 days : >2,000 mg ai/kg bw NOAEC: 2,000 mg ai/kg bw

Endpoints affected: None Practically non-toxic Supplemental 45435002

Bobwhite Quail (Colinus

virginianus ) Technical Grade LD 50 

### 14 days: >2250mg ai/kg bw* NOAEL: 2250mg ai/kg bw Endpoints affected: None Practically non-toxic Acceptable 46615501

Japanese

Quail ( Coturnix coturnix japonica ) Technical Grade LC 50 

### 8 days : >4,952 mg/kg diet NOAEC: 926 mg/kg diet Endpoint Affected: Body weight gain Slightly toxic Supplemental 45435004

Mallard Duck ( Anas platyrhynchos ) Technical Grade LC 50 

### 8 days : >5000 mg/kg diet* NOAEC: 488 mg/kg diet End point Affected: Body weight gain Practically non-toxic Acceptable 45435003

Bobwhite

Quail ( Colinus virginianus ) Technical Grade LC 50 

### 8 days: >5970 mg ai/kg diet NOAEC: 5970 mg ai/kg diet

LOAEC: >5970 mg ai/kg diet Endpoints affected: None Practically non-toxic Acceptable 46622701

Bobwhite

Quail ( Colinus virginianus ) Formulated LD 50 

### 14 days : >300 mg ai/kg bw NOAEC: 300 mg ai/kg bw Endpoints affected: None Moderately toxic Acceptable 45530602

* denotes values used to calculate RQs



## Acequinocyl chronic avian toxic ity studies indicated no parental or reproductive or offspring effects up to 2515 ppm (the highest dose test ed) in bobwhite quail (MRID 45435006) (Table 23). However, mallard duck appeared more sensitive than the bobwhite quail with a NOAEC value of 100 ppm.

## 28 Table 23. Chronic Toxicity Studies of Acequinocyl on Birds

Species Product Tested Toxicity Endpoint Study Classification MRID #

Mallard duck ( Anas platyrhynchos ) Technical Grade NOAEC: 100 mg ai/kg diet* LOAEC: 500 mg ai/kg diet Endpoints affected: females bodyweight gain Acceptable 45435005

Bobwhite quail ( Colinus virginianus ) Technical Grade NOAEC: ≥ 2515 mg ai/kg diet LOAEC: >2515 mg ai/kg diet

Endpoints affected: None Acceptable 45435006

* denotes values used to calculate RQs



## Toxicity to Mammals EFED utilizes the toxicity data from Health Effects Division on laboratory rodents and nonrodents as surrogate data for wild mammal toxic ity and risks. The acute oral gavage toxicity studies in rats and mice indicated LD 50 

## s greater than 5000 mg/kg body weight/day. Therefore acute toxicity of acequinocyl to mammals is expected to be very low (Table 24).

Table 24. Acute Toxicity Studies of Acequinocyl on Mammals

Species Product Tested Toxicity Endpoint Toxicity Classification Study Classification MRID #

Rat ( Rattus norvegicus) Technical Grade LD 50 : >5000 mg/kg-bw (males and females)* Category IV (Low Toxicity Acceptable 45435011

Mouse (Mus musculus) Technical Grade LD 50 : >5000 mg/kg-bw (males and females) Category IV (Low Toxicity) Acceptable 45435010

* denotes values used to calculate RQs



## The sub-acute (developmental) studies submitted for rat (MRID 45531908 and MRID 45651706) and rabbit (MRID 45531907 and 45651705) and two-genera tion rat study are acceptable and the results are presented in Table 25. The NOAEC from the two-generation reproduction study was used for deriving chronic mammalian risk quotients (MRID 45531909). The parental and offspring NOAEC (100 ppm for each) was more sensitive than the reproduction NOAEC of >1500 ppm.

## 29 Table 25. Developmental and Reproductive Toxi city Studies of Acequinocyl on Mammals

Species Product Tested Toxicity Endpoint Study Classification MRID #

Rabbit (Leporidae) Technical Grade Maternal NOAEL: 60 mg ai/kg bw/day LOAEL: 120 mg ai/kg bw/day Developmental NOAEL: 60 mg ai/kg bw/day LOAEL: 120 mg ai/kg bw/day Acceptable 45531907 and 45651705

Rat ( Rattus norvegicus) 

### Technical Grade Parental NOAEC (m): 100 mg/kg Diet* LOAEC (m): 800 mg/kg Diet NOAEC (f): >1500 mg/kg Diet Offspring NOAEC (m/f): 100 mg/kg Diet LOAEC(m/f): 800 mg/kg Diet Reproduction NOAEC (m/f): >1500 mg/kg Diet Acceptable 45531909

Rat ( Rattus norvegicus) 

### Technical Grade Maternal NOAEL: 150 mg ai/kg bw/day LOAEL: 500 mg ai/kg bw/day Developmental NOAEL: 500 mg ai/kg bw/day LOAEL: 750 mg ai/kg bw/day Acceptable 45531908 and 45651706

* denotes values used to calculate RQs



## Toxicity to Terrestrial Plants In the 21-day Tier I seedling emergence test, four monocots and six dicots were tested with 0 (controls), 0.3 and 0.9 lb ai/acre of formulated acequinocyl (MRID 45428609) (Table 26). Shoot height was affected by acequinocyl treatment in carrots only and EC 25 

## was estimated to be 0.7 lb ai/acre. Percent emergence, phytotoxicity, shoot height, and dry weight in acequinocyl treated groups were not significantly different from controls for monocots.

Table 26. Toxicity of Acequi nocyl to Terrestrial Plants

Species Product Tested Toxicity Endpoint Study Classification MRID #

Seedling Emergence Tier I (Monocots and Dicots) Formulated EC 25 21 days Monocots: >0.9 lb ai/A

Dicots: 0.7 lb ai/A

NOEC 21 days Monocots: 0.9 lb ai/A

Dicots: 0.3 lb ai/A Acceptable 45428609

Vegetative Vigor Tier I (Monocots and Dicots) Formulated EC 25 21 days Monocots and Dicots: >0.9 lb ai/A NOEC 21 days Monocots: 0.9 lb ai /A; Dicots: 0.3 lb ai/A Acceptable 45428610

## 30 In the 21 day Tier I vegetative vigor test, monocots and dicots were tested with 0 (controls), 0.3, 0.9 lb ai/acre of acequinocyl applied post em ergence (MRID 45428610) (Table 25). The shoot heights for only beet and cabbage were significantly different fr om controls (less than 10% for each) at 0.9 lb ai/acre. The other parameters such as phytotoxicity and plant dry weight were not affected. EC 25 

## was estimated to be greater than 0.9 lb a.i/acre for both monocots and dicots.

Toxicity to Non-target Insects Two studies, one using the technical ingredient and the other using the formulated product, were submitted by the registrant to determine the acute exposure effects of acequinocyl in honey bees. Toxicity end points, based on wh ich acequinocyl is classified as practically non-toxic to honey bees, are presented in Table 27.

Table 27. Toxicity of Acequino cyl to Non-target Insects

Species Product Tested Toxicity Endpoint Toxicity Classification Study Classification MRID #

Honey bees ( Apis mellifera) Technical Grade LD 50 

### 48 hr : >100 μ g ai/bee NOAEL: 100 μ g ai/bee (mortality) Practically nontoxic Acceptable 45435007

Honey bees ( Apis mellifera)

Honey bees ( Apis mellifera) Formulated

Formulated Contact LD 50 

### 72 hr : >350 μ g ai/bee NOAEL: 175 μ g ai/bee LOAEL: 350 μ g ai/bee (mortality)

Oral LD 50 

### 72 hr : >315 μ g ai/bee NOAEL: 43.8 μ g ai/bee LOAEL: 87.5 μ g ai/bee Practically nontoxic

Practically nontoxic Acceptable

Supplemental 45428608



## Toxicity to Earthworms Currently, OPP does not require earthworm toxicity testing for pesticide registration. However, based on an OPPTS guideline which requires ea rthworm studies be conducted for 28 days, registrant-submitted non-guideline earthworm studi es were evaluated and were classified as supplemental (Table 28).



# 4.3 Incident Database Review The EFED’s Ecological Incident Database did not contain any in cidents reported for acequinocyl.

## 31 Table 28. Toxicity of Acequinocyl to Earthworms

Species Product Tested Toxicity Endpoint Study Classification MRID #

Earthworm

(Eisenia foetida) Technical Grade LC 50 

### 14 days : > 1000 mg ai/kg NOAEC/LOAEC: 500/1000 mg ai/kg (reductions in body weight gain) Supplemental 45435009

Earthworm

(Eisenia foetida) Formulated LC 50 

### 14 days : > 156 mg ai/kg NOAEC/LOAEC: ≥ 156/>156 mg ai/kg Supplemental 45782304



# 5. Risk Estimation Data on acequinocyl’s exposure and t oxicity effects are used to de termine the risk quotients (RQ) for various species. Risk quotient, an indicator of the likelihood of adverse ecological effects on non-target species, is calculated by dividing the estimated envir onmental concentrations (EECs) by acute or chronic toxicity values. The highe st EECs and the most sensitive endpoints were used to determine a conservative estimate of risk. The resulting RQs are compared to the Agency’s pre-established levels of concern (L OCs) to determine the regulatory action. In general, higher the RQ, greater is the potential risk. Details on the RQ method and LOCs are presented in Appendix F. Appendix G summar izes the calculation of Aquatic RQs.



# 5.1 Summary of Aquatic RQs A summary of aquatic acute RQs is presented in Tables 29 and 30 below

. No acute LOC exceedances occurred for fresh water fish and fr eshwater algae with any of the crop scenarios using either model approaches. Standard RQ valu es for acute exposure to estuarine/marine fish were not calculated because the marine fish studies did not report mo rtality at the highest treatment levels tested.

Acute risk LOCs exceeded for freshwater inverteb rates for all crop scenarios except CA citrus and OR Christmas trees using model approach 1 and CA grapes, CA citrus, CA almonds, OR apples, and OR Christmas trees using model approach 2. Regardless the model approach, acute endangered species LOCs exceeded for all crop scenarios for freshwater invertebrates.

The calculated chronic risk quotients for freshwater fish and invertebrates are presented in Table 31. No chronic effects were noted on freshwater fish using either approach. However, the RQs estimated for the proposed uses of acequinocyl on NY grapes, FL citrus, GA pecans, PA and NC apples, and FL Nursery plants are above chronic LOCs for fres hwater invertebrates using the model approach 1. In model approach 2, RQs pred icted for the parent and degradate exceeded chronic LOCs for NY grapes, GA pecans, PA and NC apples, and FL Nursery plants (including the lowest application rate of 0.06 lb ai/A in roses).

Acute RQs estimated for estuarine/marine i nvertebrates exceeded the acute non-endangered species and acute endangered species LOCs rega rdless the model approach (except CA citrus using model approach 2) (Tables 29 and 30). The potential chronic risk to estuarine/marine

## 32 invertebrates was not estimated because th e mysid life cycle study was not submitted.

Table 29. Summary of Aquatic Acut e RQs Using Model Approach 1 Crop Scenario Freshwater Fish Freshwater Invertebrates Estuarine/Marine Invertebrates Freshwater Algae

------------------ ----------------- Ac ute RQs ------------

------------ ------------- ----------- CA Grapes <0.05 0.66 a,b 

## 2.68 a,b 

## <1.0 NY Grapes <0.05 2.40 a,b 

## 9.78 a,b 

## <1.0

FL Citrus <0.05 1.51 a,b 

## 6.15 a,b 

## <1.0 CA Citrus <0.05 0.13 b 

## 0.53 a,b 

## <1.0 CA Almonds <0.05 0.91 a,b 

## 3.69 a,b 

## <1.0

GA Pecan <0.05 2.35 a,b 

## 9.58 a,b 

## <1.0 OR Apples <0.05 0.95 a,b 

## 3.85 a,b 

## <1.0 PA Apples <0.05 2.74 a,b 

## 11.14 a,b 

## <1.0 NC Apples <0.05 1.57 a,b 

## 6.37 a,b 

## <1.0 OR Christmas Trees <0.05 0.37 b 

## 1.51 a,b 

## <1.0

FL Nursery Plants <0.05 2.01 a,b 

## 8.17 a,b 

## <1.0

FL Nursery Plants (Roses) <0.05 1.00 a,b 

## 4.08 a,b 

## <1.0

a 

### Equals or exceeds acute risk LOC (0.50)

b 

### Equals or exceeds acute endangered species risk LOC (0.05)



## Table 30. Summary of Aquatic Acut e RQs Using Model Approach 2 Crop Scenario Freshwater Fish Freshwater Invertebrates Estuarine/Marine Invertebrates Freshwater Algae

------------------ ----------------- Ac ute RQs ------------ ----------------------------------- CA Grapes <0.05 0.09 b 

## 0.36 b 

## <1.0 NY Grapes <0.05 1.16 a,b 

## 4.71 a,b 

## <1.0

FL Citrus <0.05 0.83 a,b 

## 3.37 a,b 

## <1.0 CA Citrus <0.05 0.08 b 

## 0.31 b 

## <1.0 CA Almonds <0.05 0.27 b 

## 1.08 a,b 

## <1.0

GA Pecan <0.05 2.10 a,b 

## 8.56 a,b 

## <1.0 OR Apples <0.05 0.34 b 

## 1.37 a,b 

## <1.0 PA Apples <0.05 1.50 a,b 

## 6.08 a,b 

## <1.0 NC Apples <0.05 1.26 a,b 

## 5.14 a,b 

## <1.0 OR Christmas Trees <0.05 0.14 b 

## 0.56 a,b 

## <1.0

FL Nursery Plants <0.05 2.32 a,b 

## 9.44 a,b 

## <1.0 FL Nursery Plants (Roses) <0.05 1.16 a,b 

## 4.73 a,b 

## <1.0

a 

### Equals or exceeds acute risk LOC (0.50)

b 

### Equals or exceeds acute endangered species risk LOC (0.05)

## 33 Table 31. Summary of Aquatic Chronic RQs Using Model Approaches 1 and 2 Crop Scenario Model Approach 1 Model Approach 2 Freshwater Fish Freshwater Invertebrates Freshwater Fish Freshwater Invertebrates CA Grapes <1.0 0.40 <1.0 0.07 NY Grapes <1.0 2.03 a 

## <1.0 1.03 a



## FL Citrus <1.0 1.18 a 

## <1.0 0.63 CA Citrus <1.0 0.11 <1.0 0.06 CA Almonds <1.0 0.49 <1.0 0.22

GA Pecan <1.0 2.01 a 

## <1.0 1.82 a 

## OR Apples <1.0 0.83 <1.0 0.28 PA Apples <1.0 2.42 a 

## <1.0 1.39 a 

## NC Apples <1.0 1.14 a 

## <1.0 1.11 a 

## OR Christmas Trees <1.0 0.28 <1.0 0.11

FL Nursery Plants <1.0 1.50 a 

## <1.0 2.44 a



## FL Nursery Plants (Roses) <1.0 0.74 <1.0 1.21 a

a 

### Equals or exceeds chronic risk LOC (1.0) 

# 5.2 Summary of Terrestrial RQs 5.2.1 Avian RQs Because there was no mortality in bi rds at the highest concentrations tested in both the acute and sub-acute toxicity tests, acute RQs were not calculated. The estimated maximum terrestrial exposure concentrations for acequinocyl are belo w the most sensitive acute oral and dietary avian LD 50 

## and LC 50 

## values of >2250 and >5000 ppm, respec tively. Therefore, the potential for acute risk to birds is not expect ed for both food and non-food uses.

Table 32 reports chronic avian RQs for the proposed food and non-food uses of acequinocyl using two different foliar dissipation half-lives. Chronic LOCs exceeded for food uses for birds of all weight classes that cons ume short grass exclusively when modeled with either half-life periods (35 or 20 days). Chronic risk LOCs, on the other hand, did not exceed for any sized birds for non-food uses with either half-life periods.

## 34 Table 32. Summary of Avian Ch ronic Risk Quotients for Food and Non-Food Uses

Food items for Birds Chronic RQs

Food uses (Two applications at 0.30 lb ai/A - 21

days apart) Non-Food Uses (Five applications

each at 0.125 lb

ai/A - 14 days apart Non-Food uses: Roses (Five applications each at 0.06 lb ai/A - 14 days apart) Modeled using foliar dissipation half-life of 35 days Short grass 1.20 a 

### 0.93 0.45 Tall grass 0.55 0.43 0.20 Broadleaf plants/small insects 0.67 0.52 0.25

Fruits/pods/seeds/lg insects 0.07 0.06 0.03 Modeled using foliar dissipation half-life of 20 days Short grass 1.07 a 

### 0.71 0.34 Tall grass 0.49 0.33 0.16 Broadleaf plants/small insects 0.60 0.40 0.19

Fruits/pods/seeds/lg insects 0.07 0.04 0.02

a 

### Equals or exceeds chronic risk LOC (1.0)



# 5.2.2 Mammalian RQs In lieu of definitive endpoints on acute toxicity of acequinocyl to mammals, the EEC’s estimated by the TREX model were compared with the most sensitive LD 50 

## values to ascertain the risk to mammals. Since the most conservative ma mmalian EEC is <114 ppm and mammalian LD 50 

## for acequinocyl is >5,000 mg/kg body wei ght, it is expected that risk due to acute exposure to acequinocyl is not an issue to herbivorous, insectivorous , or granivorous mammals.

Potential chronic risk estimates to mammals are presented in Tables 33, 34, and 35. Dose-based chronic RQs exceeded agency’s LOCs for all uses and for all mammal weight classes that feed on short grass, tall grass, broadleaf plants, and small insects regardless of half-life periods and application rates. On the othe r hand, dietary-based chronic RQs for food uses are above LOCs for mammals consuming short grass exclusively, re gardless the foliar dissipa tion half-life periods used in the modeling. There was no exceedance of chronic dietary LOC for non-food uses.

## 35 Table 33. Summary of Mammalian Ri sk Quotients for Food Uses

Chronic Dose-Based RQs Chronic DietaryBased RQs Risk Quotients Based on Kenaga Upper Bound EEC 15 g 35 g 1000 g All Mammals Acequinocyl half-life 35 days; two applications each at 0.30 lb ai/A 21 days apart Short grass 10.37 a 

### 8.86 a 

### 4.75 a 

### 1.20 b 

### Tall grass 4.75 a 

### 4.06 a 

### 2.18 a 

### 0.55 Broadleaf plants/

small insects 5.83 a 

### 4.98 a 

### 2.67 a 

### 0.67 Fruits/pods/seeds/ lg insects 0.65 0.55 0.30 0.07 Seeds (granivores) 0.14 0.12 0.07 Acequinocyl half-life 20 days; two applications each at 0.30 lb ai/A 21 days apart Short grass 9.26 a 

### 7.91 a 

### 4.24 a 

### 1.07 b 

### Tall grass 4.25 a 

### 3.63 a 

### 1.94 a 

### 0.49 Broadleaf plants/

small insects 5.21 a 

### 4.45 a 

### 2.39 a 

### 0.60 Fruits/pods/seeds/ lg insects 0.58 0.49 0.27 0.07 Seeds (granivores) 0.13 0.11 0.06

a 

### Equals or exceeds chronic risk LOC (1.0)



## Table 34. Summary of Mammalian Risk Quotients for Non-Food Uses

Chronic Dose-Based RQs Chronic DietaryBased RQs Risk Quotients Based on Kenaga Upper Bound EEC 15 g 35 g 1000 g All Mammals Acequinocyl half-life 35 days; five applications each at 0.125 lb ai/A 14 days apart Short grass 8.06 a 

### 6.89 a 

### 3.69 a 

### 0.93 Tall grass 3.70 a 

### 3.16 a 

### 1.69 a 

### 0.43 Broadleaf plants/

small insects 4.53 a 

### 3.87 a 

### 2.08 a 

### 0.52 Fruits/pods/seeds/ lg insects 0.50 0.43 0.23 0.06 Seeds (granivores) 0.11 0.10 0.05 Acequinocyl half-life 20 days; five applications each at 0.125 lb ai/A 14 days apart Short grass 6.17 a 

### 5.27 a 

### 2.83 a 

### 0.71 Tall grass 2.83 a 

### 2.42 a 

### 1.30 a 

### 0.33 Broadleaf plants/

small insects 3.47 a 

### 2.97 a 

### 1.59 a 

### 0.40 Fruits/pods/seeds/ lg insects 0.39 0.33 0.18 0.04 Seeds (granivores) 0.09 0.07 0.04

a 

### Equals or exceeds chronic risk LOC (1.0)

## 36 Table 35. Summary of Mammalian Risk Qu otients for Non-Food Uses (Roses)

Chronic Dose-Based RQs Chronic DietaryBased RQs Risk Quotients Based on Kenaga Upper Bound EEC 15 g 35 g 1000 g All Mammals Acequinocyl half-life 35 days; five applications each at 0.06 lb ai/A 14 days apart Short grass 3.87 a 

### 3.31 a 

### 1.77 a 

### 0.45 Tall grass 1.77 a 

### 1.52 a 

### 0.81 a 

### 0.20 Broadleaf plants/

small insects 2.18 a 

### 1.86 a 

### 1.00 a 

### 0.25 Fruits/pods/seeds/ lg insects 0.24 0.21 0.11 0.03 Seeds (granivores) 0.05 0.05 0.02 Acequinocyl half-life 20 days; five applications each at 0.06 lb ai/A 14 days apart Short grass 2.96 a 

### 2.53 a 

### 1.36 a 

### 0.34 Tall grass 1.36 a 

### 1.16 a 

### 0.62 a 

### 0.16 Broadleaf plants/

small insects 1.67 a 

### 1.42 a 

### 0.76 a 

### 0.19 Fruits/pods/seeds/ lg insects 0.19 0.16 0.08 0.02 Seeds (granivores) 0.04 0.04 0.02

a 

### Equals or exceeds chronic risk LOC (1.0)



# 5.2.3 Plant RQs Since the terrestrial EECs (0.0012 to 0.006 lb ai/A ) are several fold lower than the seedling emergence NOAECs (0.9 lb ai/acre for monocots a nd 0.3 lb ai/acre for di cots), the potential acute risk to non-endangered plan ts and endangered plants are expe cted to be below levels of

concern.



# 5.2.4 Insects Currently, EFED does not quantify the potential risk of pesticides on terrestrial non-target insects. However, based on the guideline data available on honey bee toxicity, acequinocyl is practically non-toxic to honey bees (LD 50 

## = >100 μ g/bee). Therefore, th e potential risk of acequinocyl to pollinators a nd other beneficial insects is expected to be low.



# 6. Risk Characterization Risk characterization integrates exposure and effects data to determine the ecological risk resulting from the use of acequinocyl and the likelihood of impacts on aq uatic and terrestrial biota. Risk characterization pr ovides estimation and description of the risk; articulates risk assessment assumptions, limitations, and uncertainti es; synthesizes an overall conclusion; and provides the risk managers with informa tion to support regulatory decision making.

Acequinocyl environmental concentration estimates and calculated RQs are different in this assessment compared to the initial assessment co mpleted in 2003. This is due to the use of new scenarios as a result of expanded label uses in new crops, modifications to crop planting dates for some existing scenarios, selection of toxicity endpoints from the submitted new studies in certain

## 37 cases, and the use of the model TREX in the place of ELL-FATE.



# 6.1 Aquatic Risk 6.1.1 Animals The predicted aquatic concentrations for acequi nocyl are below the solubility limit of the chemical (6.7 ppb) and are lower than the reported LC 50 

## values for fish (maximum peak EEC of 6.57 ppb in model approach 1 for PA apples and 5.05 ppb in model approach 2 for GA pecan versus rainbow trout LC 50 

## of 67,000 ppb and sheepshead minnow LC 50 

## of 190 ppb). Therefore the proposed food and non-food uses for acequinoc yl may not pose a potential for acute and chronic risk to both freshwater a nd marine fish. Also, potential chroni c risk to freshwater fish is not expected since the NOAEC fo r rainbow trout of 520 ppb is we ll above the estimated aquatic concentrations. Based on the above and in the ab sence of studies on chronic exposure effects of acequinocyl in estuarine/marine fi sh, it is expected that acequi nocyl use may not pose any risk due to chronic exposure to estuarine/marine fish.

Regardless the application rate for the propos ed food and non-food uses, acequinocyl is very highly toxic to both freshwater and estuarine/marine invertebra tes. Based on the toxicity end points generated from various studies, it appear s that acequinocyl is much more toxic to estuarine/marine invertebrates (EC 50 

## of 0.59 ppb for Eastern oysters and LC 50 

## of 0.82 ppb for mysid shrimp) compared to freshwater invertebrates (LC 50 

## of 2.4 ppb for daphnids). All of the proposed uses for acequinocyl, regardless of th e modeling approach, pose potential acute and acute endangered species risk to aquatic invertebrates.

Acequinocyl concentrations to which aquatic inve rtebrates could be expos ed in a natural water setting will vary considerably depending on the type of water body. The water body modeled by EXAMS is static with no downstream dilution e ffects. Therefore, specific effects acequinocyl may exert on aquatic invertebrates and the extent of resulting risk is difficult to predict. However, based on 21-day and 60-day average EECs derived from both model approaches, risk appear to exceed for estuarine/marine invertebrate s compared to freshwater invertebrates.

Acequinocyl’s potential for off-site movement is gene rally low as it is strongly adsorbed to soils. If acequinocyl moves from target area to the n earby aquatic areas, potential exists for its sorption to soil sediments which could result in adverse impacts to the benthic community or the bottomdwelling organisms that feed and live in/on the bo ttom. In view of acequino cyl’s half-life of less than 2 days in aerobic soils, however, adsorpti on to soil sediments is not a big concern.

To determine if sediment-dwelling organisms c ould be adversely affected from the proposed uses, acequinocyl’s peak concentration in benthic pore water was calculated using PRZM/ EXAMS. Benthic pore water concentrations fo r various scenarios ranged between 0.11 to 1.69 ppb using model approach 1 and 0.02 to 2.5 ppb usi ng model approach 2 (data not presented). Since the submitted toxicity endpoint (LC 50 

## : >86,000 ppb) for the sediment-dwelling Chironomus riparius is several orders of magnitude highe r than the pore water concentrations, it is expected that proposed uses for acequinocyl may not pose any risk to sediment-dwelling

## 38 organisms. However, the registrant-submitted study on chironomids (MRID 45782303) was not conducted according to the sediment toxicity guideline (850.1735) as it failed to maintain sediment in the water column. Therefore, a ne w study is required to evaluate the impact of acequinocyl on sediment-dwe lling invertebrates.

Theoretically, use of reduced application rates, fewer applications, longer application intervals, and vegetative buffer strips could be employed to mitigate risk associat ed with a pesticide. However, in view of LOC exceedances occurring even with the lowest non-food use rate of 0.06 lb/A for roses, reduction of a pplication rate is not a viab le option for acequinocyl. TREX simulations (discussed below) suggest that decreasing the frequency and overall number of applications are not viable opti ons either. Maintaining vegetative buffers between treated areas and water bodies could help alleviate risk from acequinocyl to aqua tic invertebrates.



# 6.1.1.1 Risks Based on the Probit Slope Analysis The probit dose-response slope was used to calc ulate the chance of an individual mortality corresponding to the listed invertebrate specie s acute LOCs or RQs (Table 36). Chance of individual effects was not calculated for fish as none of the guideline test s showed mortality as the endpoint. Where slope estimates are unavaila ble from a study, a default slope of 4.5 was used. The analysis, which uses the EFED spread sheet IEC (version 1.1.xls), can go as low as 1 x 10 -16 

## in estimating the event probability for animals.

Table 36. Chance of Individual Effects for Inv ertebrates Based on Acute Aquatic RQs and Models 1 and 2 a 

## Scenario Slope RQs for Freshwater Invertebrates Chance of Effects for Freshwater Invertebrates Slope RQs for Marine Invertebrates Chance of Effects for

Marine Invertebrates 1 in ... 1 in ... CA Grapes 6.2 0.66 (0.09) 7.6 (2.23E+10) 4.5 2.68 (0.36) 1.03 (4.36E+01) NY Grapes 6.2 2.40 (1.16) 1.01 (1.53) 4.5 9.78 (4.71) 1 (1) FL Citrus 6.2 1.51 (0.83) 1.15 (3.25) 4.5 6.15 (3.37) 1 (1) CA Citrus 6.2 0.13 (0.08) 5.08 E+07 (1.92 (E+11) 4.5 0.53 (0.31) 9.32 (9.06E+01) CA Almonds 6.2 0.91 (0.27) 2.5 (4.73E+03) 4.5 3.69 (1.08) 1.01 (1.79)

GA Pecan 6.2 2.35 (2.10) 1.01 (1.02) 4.5 9.58 (8.56) 1 (1) OR Apples 6.2 0.95 (0.34) 2.25 (5.44E+02) 4.5 3.85 (1.37) 1 (1.37) PA Apples 6.2 2.74 (1.50) 1 (1.16) 4.5 11.14 (6.08) 1 (1) NC Apples 6.2 1.57 (1.26) 1.13 (1.36) 4.5 6.37 (5.14) 1 (1) OR Christmas Trees 6.2 0.37 (0.14) 2.69E+02 (1.67E+07) 4.5 1.51 (0.56) 1.27 (7.78)

FL Nursery Plants 6.2 2.01 (2.32) 1.03 (1.01) 4.5 8.17 (9.44) 1 (1) FL Nursery Plants (Roses) 6.2 1.0 (1.16) 2 (1.53) 4.5 4.08 (4.73) 1 (1)

a RQs and individual mortalit y chance estimates for Model 2 are in parenthesis

## 39 The slope used for the LC 50 

## of the most sensitive acute freshwater invertebrate ( Daphnia magna ; LC 50 

## = 2.4 μg ai/L) and estuarine/marine invertebrate (Eastern oyster; EC 50 

## = 0.59 μg ai/L) was 6.2 and 4.5 (default value), resp ectively. Using the acute endangered species LOC of 0.05, the chance of an individual mortalit y for aquatic-phase freshwater a nd estuarine/marine invertebrates is estimated to be 2.76E+15 and 4.18E+08, respec tively. Based on an anal ysis of the likelihood of individual mortality using the highest RQ value (estuarine/marine invertebrates RQ = 11.14, PA apples scenario, model 1), the likelihood of i ndividual mortality is 100% (Table 36). At the lowest RQ value of 0.08 (CA citrus scenario, mode l 2), the likelihood of i ndividual mortality is ~ 1 in 1.92E+11.



# 6.1.2 Plants Aquatic non-vascular (algal) plan ts are not at risk from acequi nocyl uses. The acute peak EECs modeled for the proposed use of acequinocyl for various uses ranged between 0.18 and 6.57 ppb and are several magnitude orders lower than the EC 50 

## reported for freshwater algae (EC 50 

## = 960 ppb). Therefore, the acute RQs for both enda ngered and non-endangered species are well below the acute LOCs for aquatic plants.



# 6.2 Terrestrial Risk 6.2.1 Animals (Birds, Mammals, and Insects) Acequinocyl exhibits low toxici ty to honey bees and terrestria l insects. However, acequinocyl has the potential to negatively impact birds and mammals.

Because the avian (oral and diet ary) and mammalian (oral) acute studies did not establish definitive endpoints as no mortality was noted with the highest tested concentrations, standard RQ values for acute exposure were not calculated in the Risk Estimation section of this assessment. In order to gain a better understa nding of how the EECs for the maximum proposed acequinocyl application rate (for both food and non-food uses) re late to the toxicity data currently available for birds and mammals, TR EX was used to calculate RQs using the conservative assumption that the highest concen tration tested in the avian and mammalian studies represent endpoints ( i.e ., acute: LD 50 

## s = 2250 mg/kg bw and 5000 mg/kg diet for birds; 5000 mg/kg bw for mammals). In this exercise all of the acute RQs calculated using upper bound Kenaga values were less than 0.1 for all size a nd dietary classes for both food and non-food uses. Therefore, direct risk to non-listed and liste d birds and mammals from acute exposure to acequinocyl is low.

The potential for adverse effects to mammals a ppear to be likely based on chronic dose-based RQs. Dose-based mammalian RQs ranged between 1 and 10. Chronic diet ary-based exceedances occurred for food uses only (i.e., with the 0.3 lb ai/A rate) for both birds and mammals that feed on short grass exclusively. The chronic dietar y-based risk quotients ranged from 1.07 to 1.2, suggesting that risk due to acequinocyl may be bord er-line. Furthermore, it is unlikely that birds and mammals will feed exclusively on shortgrass.

## 40 TREX simulations suggest that none of the mitiga tion measures such as reduction of application rate, application frequency, and number of applic ations could reduce risk to mammals. Reducing acequinocyl’s rate of applicati on is not a viable option in view of LOC exceedances for mammals even with the lowest use rate of 0.06 lb ai/A (roses). Reducing the number of acequinocyl applications from 2 and 5 for food uses and non-food uses, respectively, to 1 or decreasing the frequency of applications did no t reduce the risk quotients below 1.



# 6.2.1.1 Risks Based on the Probit Slope Analysis Te likelihood of individual avian and mamm alian mortality resulting from the proposed acequinocyl uses is not calculat ed as mortality was not observe d in the guideline studies.



# 6.2.2 Plants As can be expected with an insecticide, acequi nocyl use does not appear to pose any risk to terrestrial plants. The agency’s LOC did not exceed for both food and non-food uses due to either runoff or drift of acequinocyl.



# 6.3 Endangered Species Endangered species that could poten tially be affected by the propos ed uses of acequinocyl were identified using the searchable LOCATES da tabase. EFED’s screening-level assessment considers broadly described taxonomic groups and assumes that aquatic organisms in a surface water body and terrestrial plants and wildlife are located on or ad jacent to the tr eated site. The assessment also assumes that this adjacent area has highest potential exposure to the pesticide and that exposures are likely to decrease with increasing distance from the treatment area.

The State-by-state list of threatened and endange red species that could po tentially be affected by acequinocyl use is provided in Appendix H. A to tal of 1144 organisms in the taxa that included mammals, birds, amphibians, reptiles, fish, crustaceans, bivalves, gastropods, and insects are identified to be at risk from the proposed new uses of acequinocyl (Table 37). Endangered species were identified for taxa other than thos e that exceeded agency’ s LOC in view of the indirect effects that loss of a certain species may cause on other links of the food chain.



# 6.3.1 Aquatic Endangered Species The screening-level risk assessment for acequino cyl identified potential concerns for listed freshwater and estuarine/marine invertebrates. Currently, there are no fede rally listed endangered estuarine/marine invertebrates. However, fres hwater invertebrates ma y be affected by the proposed acequinocyl uses.



# 6.3.2 Terrestrial Endangered Species The current risk assessment suggests that acequi nocyl has the potential to impact endangered birds and mammals. Since birds serve as surrogate species for terrestrial phase amphibians and reptiles, it is expected that amphibians and reptiles are also at risk. About 472 terrestrial endangered animal species (144 mammals, 231 bi rds, 22 amphibians, and 75 reptiles) are estimated to be present in states where acequino cyl applications will be made (Table 37).

## 41 Table 37. Endangered Species Occurring in the States Planted to Proposed Crops State Number of Endangered Species in the Stat es with Proposed Acequinocyl Uses

Mammals Birds Amphibians Fish Reptiles Crustaceans Bivalves Gastropods Arachnids Insects

AL 4 4 2 15 5 1 30 10 0 0 AZ 9 8 2 18 2 0 0 1 0 0 AR 3 3 0 3 0 2 6 1 1 1 CA 20 16 6 31 8 9 0 1 0 22 CO 2 3 0 6 0 0 0 0 0 2 CT 1 3 0 1 1 0 1 0 0 1 DE 1 2 0 1 1 0 0 0 0 0 FL 13 10 1 4 10 1 7 1 0 1 GA 3 5 1 11 2 0 16 0 0 1 HI 1 32 0 0 2 1 0 39 0 1 ID 4 2 0 8 0 0 0 6 0 0 IL 2 3 0 1 0 1 7 1 0 2 IN 2 3 0 0 1 0 11 0 0 2 IA 1 3 0 2 0 0 2 1 0 0 KS 2 4 0 4 0 0 0 0 0 1 KY 3 7 0 4 0 1 22 0 0 1 LA 1 5 0 2 7 0 3 0 0 0 ME 1 3 0 2 0 0 0 0 0 0 MD 2 2 0 2 1 0 1 0 0 2 MA 1 4 0 1 2 0 0 0 0 3 MI 3 3 0 0 1 0 2 0 0 4 MN 2 2 0 1 0 0 2 0 0 0 MS 3 6 1 3 7 0 8 0 0 1 MO 2 3 0 7 0 1 6 1 0 0 MT 3 4 0 5 0 0 0 0 0 2 NC 4 5 0 4 5 0 8 1 1 1 ND 0 4 0 1 0 0 0 0 0 0 NE 1 4 0 2 0 0 0 0 0 1 NH 1 1 0 0 0 0 1 0 0 1 NJ 1 3 0 1 1 0 0 0 0 0 NM 5 7 1 13 1 2 0 5 0 0 NV 0 3 0 23 1 0 0 0 0 2 NY 1 3 0 1 1 0 1 1 0 1 OH 2 2 0 1 2 0 6 0 0 4 OK 3 7 0 4 0 0 2 0 0 1 OR 1 5 0 22 0 1 0 0 0 2 PA 2 2 0 0 1 0 2 0 0 0 RI 1 1 0 1 0 0 0 0 0 1 SC 1 5 1 1 5 0 1 0 0 0 SD 1 4 0 2 0 0 38 0 0 1 TN 3 4 0 16 0 0 0 3 1 0 TX 5 13 4 9 6 1 0 1 10 0 UT 2 3 0 8 1 1 0 0 0 0 VT 1 1 0 0 0 0 1 0 0 0 VA 5 3 1 7 1 2 21 1 0 0 WA 5 5 0 19 0 0 0 0 0 0 WV 5 1 1 0 0 0 5 1 0 0 WI 2 4 0 2 0 0 2 0 0 0 WY 4 1 1 0 0 0 0 0 0 0 Total 144 231 22 270 75 24 213 75 13 77

## 42 

# 6.3.3 Direct and Indirect Effects The summary of potential direct a nd indirect effects to endangered species is presented in Table 38. In view of the sensitivity of aquatic invert ebrates and terrestrial phase birds, mammals, amphibians, and reptiles to acequinocyl, direct impact of its use would be loss of the above species due to mortality. The loss of these species will also result in structural and functional changes of both the aquatic and terrestrial ecosystems. Importantly, changes will be manifested in the form of reduced biodivers ity and disruption of food chain.

Biodiversity or species diversity ensures that ecosystems survive though the flows of energy. A change in the life of one speci es, especially thro ugh extinction, could ripple throughout an ecosystem, changing the life for many other spec ies. Through these ripple effects, the loss of above species is likely to cause indirect effect s such as loss of productivity and stability leading to multiple complex imbalances in ecosystems they thrive in. For example, if invertebrates are lost in a particular ecosystem, insect larvae and fi sh that feed on inverteb rates will be affected first. Negative impacts on fish and insect larv ae trigger indirect food web-related effects on higher-level organisms such as bi g fish, birds, and amphibians.

Table 38. Summary of Potential Direct and Indi rect Effects to Federally-Listed Taxa Organism Direct Effects Indirect Effects 1 

## Terrestrial and semi-aquatic monocots and dicots No Yes

Terrestrial invertebrates No Yes Birds Yes Yes Terrestrial phase amphibians Yes Yes Reptiles Yes Yes Mammals Yes Yes Aquatic vascular plants No Yes Freshwater fish No Yes Aquatic phase amphibians No Yes Estuarine/marine fish No Yes Freshwater invertebrates Yes Yes Estuarine/marine invertebrates Yes Yes

1 

### Specific indirect effects were not identifie d as refined analysis was not conducted

## 43 References

Fletcher, J.S., J.E. Nellessen, and T.G. Pfleeger . 1994. Literature review and evaluation of the EPA food-chain (Kenaga) nomogram, an instrument for estimating pesticide residues on plants. Env. Toxicol. Chem. 13:1381-1391.

Hoerger, F. and E. E. Kenaga. 1972. Pesticide re sidues on plants: correlati on of representative data as a basis for estimation of th eir magnitude in the environment. in : F. Coulston and F. Korte (editors), Environmental Quality and Safety: Chem istry, Toxicology, and Technology. Vol I. Georg Thieme Publishers, Stut tgart, West Germany, pp. 9-28.

Pfleeger, T.G., A. Fong, R. Hayes, H. Ratsch and C. Wickliff. 1996. Field evaluation of the EPA (Kenaga) nomogram, a method for estimati ng wildlife exposure to pesticide residues on plants. Env. Toxicol. Chem. 15:535-543, 1996.

USDA’s Crop Profiles. Accessed from http://c ipm.ncsu.edu/cropprofiles/cplist.cfm?org=crop.

USDA-NASS. Multiple years. Crop Produc tion. Accessed from http://www.nass.usda.gov /Statistics_by_Subject/index.asp

## 44 

# Appendix A. Aquatic Expo sure PRZM-EXAMS Outputs

CA Almonds – Model Approach 1

stored as ACAalmd1.out Chemical: Acequinocyl PRZM environment: CAalmond_N irrigC.txt modified Thuday, 17 June 2004 at 09:13:20 EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 17:33:30 Metfile: w23232.dvf modified Wedday, 3 July 2002 at 10:04:22 Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 0.2096 0.1224 0.0475 0.02466 0.01933 0.00976 1962 1.706 0.9844 0.3738 0.2249 0.1881 0.07952 1963 0.7144 0.4939 0.2627 0.2065 0.1972 0.1618 1964 0.7818 0.4995 0.2452 0.1845 0.1699 0.1377 1965 0.2665 0.1889 0.1422 0.1319 0.1279 0.1089 1966 0.2988 0.1961 0.1407 0.1048 0.09053 0.08023 1967 1.724 1.038 0.4245 0.2666 0.2376 0.1671 1968 0.3542 0.2513 0.1622 0.1442 0.1389 0.1142 1969 0.7027 0.4691 0.2533 0.2039 0.1863 0.14 1970 1.653 1.138 0.4304 0.2344 0.1888 0.1427 1971 0.2992 0.2215 0.1774 0.1719 0.1687 0.141 1972 0.4264 0.2768 0.1746 0.1248 0.1122 0.1021 1973 0.9792 0.6016 0.2793 0.2386 0.2169 0.1647 1974 0.2733 0.2249 0.1434 0.1375 0.1349 0.1176 1975 0.3729 0.2456 0.158 0.1301 0.1208 0.09957 1976 0.2275 0.1493 0.08499 0.07725 0.07481 0.06738 1977 0.4558 0.2828 0.1696 0.1054 0.08604 0.06502 1978 1.03 0.7543 0.4309 0.3066 0.2758 0.196 1979 0.819 0.5272 0.2951 0.2353 0.2255 0.1758 1980 0.8868 0.5776 0.3387 0.2609 0.2398 0.1793 1981 1.666 1 0.4343 0.2968 0.2492 0.1886 1982 3.073 1.794 0.6945 0.4877 0.4669 0.3931 1983 1.443 0.9415 0.4771 0.4506 0.4266 0.3675 1984 0.4156 0.3734 0.349 0.333 0.3205 0.259 1985 1.093 0.6665 0.3026 0.2081 0.2011 0.1754 1986 3.183 2.202 0.8242 0.5054 0.4367 0.2859 1987 0.9154 0.5931 0.3196 0.2685 0.2516 0.2041 1988 0.3692 0.2727 0.1935 0.172 0.1651 0.1449 1989 0.7245 0.4403 0.1986 0.1518 0.1492 0.1231 1990 2.234 1.309 0.4847 0.3424 0.2999 0.2224

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258064516129 3.183 2.202 0. 8242 0.5054 0.4669 0.3931 0.0645161290322581 3.073 1.794 0. 6945 0.4877 0.4367 0.3675 0.0967741935483871 2.234 1.309 0. 4847 0.4506 0.4266 0.2859 0.129032258064516 1.724 1.138 0. 4771 0.3424 0.3205 0.259 0.161290322580645 1.706 1.038 0. 4343 0.333 0.2999 0.2224 0.193548387096774 1.666 1 0.4309 0.3066 0.2758 0.2041 0.225806451612903 1.653 0.9844 0. 4304 0.2968 0.2516 0.196 0.258064516129032 1.443 0.9415 0. 4245 0.2685 0.2492 0.1886 0.290322580645161 1.093 0.7543 0. 3738 0.2666 0.2398 0.1793 0.32258064516129 1.03 0.6665 0.349 0.2609 0.2376 0.1758 0.354838709677419 0.9792 0.6016 0. 3387 0.2386 0.2255 0.1754 0.387096774193548 0.9154 0.5931 0. 3196 0.2353 0.2169 0.1671 0.419354838709677 0.8868 0.5776 0. 3026 0.2344 0.2011 0.1647 0.451612903225806 0.819 0.5272 0. 2951 0.2249 0.1972 0.1618

## 45 0.483870967741936 0.7818 0.4995 0. 2793 0.2081 0.1888 0.1449 0.516129032258065 0.7245 0.4939 0. 2627 0.2065 0.1881 0.1427 0.548387096774194 0.7144 0.4691 0. 2533 0.2039 0.1863 0.141 0.580645161290323 0.7027 0.4403 0. 2452 0.1845 0.1699 0.14 0.612903225806452 0.4558 0.3734 0. 1986 0.172 0.1687 0.1377 0.645161290322581 0.4264 0.2828 0. 1935 0.1719 0.1651 0.1231 0.67741935483871 0.4156 0.2768 0. 1774 0.1518 0.1492 0.1176 0.709677419354839 0.3729 0.2727 0. 1746 0.1442 0.1389 0.1142 0.741935483870968 0.3692 0.2513 0. 1696 0.1375 0.1349 0.1089 0.774193548387097 0.3542 0.2456 0. 1622 0.1319 0.1279 0.1021 0.806451612903226 0.2992 0.2249 0. 158 0.1301 0.1208 0.09957 0.838709677419355 0.2988 0.2215 0. 1434 0.1248 0.1122 0.08023 0.870967741935484 0.2733 0.1961 0. 1422 0.1054 0.09053 0.07952 0.903225806451613 0.2665 0.1889 0. 1407 0.1048 0.08604 0.06738 0.935483870967742 0.2275 0.1493 0. 08499 0.07725 0.07481 0.06502 0.967741935483871 0.2096 0.1224 0. 0475 0.02466 0.01933 0.00976

0.1 2.183 1.2919 0.48394 0.43978 0.41599 0.28321 Average of yearly averages: 0.160479333333333

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: ACAalmd1 Metfile: w23232.dvf PRZM scenario: CAalmond_NirrigC.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.9e-5 atm-m^3/mol Vapor Pressure vapr 1.268e-6 torr Solubility sol 0.067 mg/L Kd Kd 678 mg/L Koc Koc mg/L Photolysis half-life kdp 0 days Half-life Aerobic Aquatic Metabolism kbacw 0 days Halfife Anaerobic Aquatic Metabolism kbacs 0 days Halfife Aerobic Soil Metabolism asm 0 days Halfife Hydrolysis: pH 7 0 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.34 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 15-06 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 21 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none none, mont hly or total(average of entire run)

## 46 

# CA Almonds – Model Approach 2

stored as ACAalmd2.out Chemical: Acequinocyl PRZM environment: CAalmond_N irrigC.txt modified Thuday, 17 June 2004 at 09:13:20 EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 17:33:30 Metfile: w23232.dvf modified Wedday, 3 July 2002 at 10:04:22 Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 0.4614 0.358 0.1629 0.06249 0.04183 0.01601 1962 3.872 2.94 1.287 0.4962 0.3385 0.09577 1963 0.2462 0.1931 0.09758 0.04576 0.03476 0.01947 1964 0.2863 0.2147 0.0735 0.03539 0.0269 0.01718 1965 0.1752 0.1278 0.04893 0.0354 0.02542 0.009586 1966 0.2007 0.1548 0.09755 0.05549 0.03717 0.01545 1967 0.2757 0.219 0.1065 0.04256 0.02967 0.01406 1968 0.1755 0.1276 0.04862 0.03428 0.02322 0.009365 1969 0.1754 0.1276 0.06342 0.03417 0.02318 0.01419 1970 0.7582 0.6023 0.2677 0.1059 0.07077 0.02682 1971 0.1758 0.1282 0.04911 0.0347 0.02354 0.007719 1972 0.2123 0.1637 0.09292 0.04438 0.03117 0.01408 1973 0.1758 0.1277 0.04857 0.0345 0.02339 0.01522 1974 0.2151 0.1665 0.07424 0.04378 0.02971 0.008816 1975 0.175 0.1269 0.04803 0.03357 0.02273 0.006491 1976 0.1745 0.126 0.04725 0.03281 0.02223 0.005788 1977 0.1753 0.127 0.05737 0.03424 0.02301 0.01221 1978 0.176 0.1326 0.08977 0.04301 0.0304 0.01641 1979 0.1756 0.1282 0.04928 0.03454 0.02341 0.01231 1980 0.1756 0.128 0.04899 0.03444 0.02334 0.00996 1981 0.6513 0.4917 0.2198 0.1103 0.0751 0.02777 1982 0.3591 0.2781 0.139 0.07314 0.05428 0.02841 1983 0.2737 0.2178 0.122 0.0628 0.04208 0.01926 1984 0.1762 0.1299 0.05095 0.03569 0.02421 0.00837 1985 0.3228 0.2487 0.112 0.04288 0.02877 0.01342 1986 0.1753 0.1315 0.05719 0.03349 0.02272 0.01058 1987 0.1752 0.1274 0.04848 0.03399 0.02305 0.008471 1988 0.1756 0.1277 0.04857 0.03442 0.02477 0.007972 1989 0.5877 0.4392 0.1769 0.07253 0.05378 0.01957 1990 0.2266 0.176 0.07571 0.03424 0.02354 0.01192

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258064516129 3.872 2.94 1. 287 0.4962 0.3385 0.09577 0.0645161290322581 0.7582 0.6023 0. 2677 0.1103 0.0751 0.02841 0.0967741935483871 0.6513 0.4917 0. 2198 0.1059 0.07077 0.02777 0.129032258064516 0.5877 0.4392 0. 1769 0.07314 0.05428 0.02682 0.161290322580645 0.4614 0.358 0. 1629 0.07253 0.05378 0.01957 0.193548387096774 0.3591 0.2781 0. 139 0.0628 0.04208 0.01947 0.225806451612903 0.3228 0.2487 0. 122 0.06249 0.04183 0.01926 0.258064516129032 0.2863 0.219 0. 112 0.05549 0.03717 0.01718 0.290322580645161 0.2757 0.2178 0. 1065 0.04576 0.03476 0.01641 0.32258064516129 0.2737 0.2147 0. 09758 0.04438 0.03117 0.01601 0.354838709677419 0.2462 0.1931 0. 09755 0.04378 0.0304 0.01545 0.387096774193548 0.2266 0.176 0. 09292 0.04301 0.02971 0.01522 0.419354838709677 0.2151 0.1665 0. 08977 0.04288 0.02967 0.01419 0.451612903225806 0.2123 0.1637 0. 07571 0.04256 0.02877 0.01408 0.483870967741936 0.2007 0.1548 0. 07424 0.03569 0.0269 0.01406 0.516129032258065 0.1762 0.1326 0. 0735 0.0354 0.02542 0.01342 0.548387096774194 0.176 0.1315 0. 06342 0.03539 0.02477 0.01231

## 47 0.580645161290323 0.1758 0.1299 0. 05737 0.0347 0.02421 0.01221 0.612903225806452 0.1758 0.1282 0. 05719 0.03454 0.02354 0.01192 0.645161290322581 0.1756 0.1282 0. 05095 0.0345 0.02354 0.01058 0.67741935483871 0.1756 0.128 0. 04928 0.03444 0.02341 0.00996 0.709677419354839 0.1756 0.1278 0. 04911 0.03442 0.02339 0.009586 0.741935483870968 0.1755 0.1277 0. 04899 0.03428 0.02334 0.009365 0.774193548387097 0.1754 0.1277 0. 04893 0.03424 0.02322 0.008816 0.806451612903226 0.1753 0.1276 0. 04862 0.03424 0.02318 0.008471 0.838709677419355 0.1753 0.1276 0. 04857 0.03417 0.02305 0.00837 0.870967741935484 0.1752 0.1274 0. 04857 0.03399 0.02301 0.007972 0.903225806451613 0.1752 0.127 0. 04848 0.03357 0.02273 0.007719 0.935483870967742 0.175 0.1269 0. 04803 0.03349 0.02272 0.006491 0.967741935483871 0.1745 0.126 0. 04725 0.03281 0.02223 0.005788

0.1 0.64494 0.48645 0.21551 0.102624 0.069121 0.027675 Average of yearly averages: 0.0167549333333333

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: ACAalmd2 Metfile: w23232.dvf PRZM scenario: CAalmond_NirrigC.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.9e-5 atm-m^3/mol Vapor Pressure vapr 1.268e-6 torr Solubility sol 0.067 mg/L Kd Kd 27 mg/L Koc Koc mg/L Photolysis half-life kdp 0.19 days Half-life Aerobic Aquatic Metabolism kbacw 97 days Halfife Anaerobic Aquatic Metabolism kbacs 649.8 days Halfife Aerobic Soil Metabolism asm 48.5 days Halfife Hydrolysis: pH 7 9.6 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.34 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 15-06 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 21 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none none, mont hly or total(average of entire run)

## 48 

# CA Citrus – Model Approach 1

Stored as ACAcitr1.out Chemical: Acequinocyl PRZM environment: CAcitrus_N irrigC.txt modified Thuday , 17 June 2004 at 09:14:54 EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 17:33:30 Metfile: w23155.dvf modified Wedday, 3 July 2002 at 10:04:20 Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 0.1719 0.09507 0.03118 0.02289 0.01846 0.007852 1962 0.1814 0.1042 0.04029 0.03233 0.02771 0.01738 1963 0.1854 0.1086 0.04452 0.03636 0.03168 0.02159 1964 0.1861 0.109 0.04497 0.03705 0.03235 0.02192 1965 0.1855 0.1082 0.04431 0.0363 0.03154 0.02144 1966 0.1885 0.1112 0.04724 0.03928 0.03446 0.02499 1967 0.1898 0.1133 0.04897 0.04088 0.03597 0.02558 1968 0.1892 0.1126 0.04833 0.04038 0.03565 0.0256 1969 0.1919 0.1144 0.05038 0.04278 0.03789 0.02777 1970 0.1911 0.1132 0.04934 0.04166 0.03674 0.02687 1971 0.1945 0.1157 0.0583 0.0451 0.03923 0.0263 1972 0.1876 0.1205 0.05077 0.04016 0.03503 0.02434 1973 0.1926 0.1146 0.05078 0.04292 0.03798 0.02918 1974 0.1915 0.1142 0.05013 0.04229 0.0374 0.02958 1975 0.1959 0.1184 0.05434 0.04658 0.04159 0.03176 1976 0.1905 0.1122 0.0485 0.04093 0.03593 0.02623 1977 0.198 0.1182 0.04821 0.04028 0.03536 0.02672 1978 3.423 2.283 0.7693 0.4279 0.3611 0.2201 1979 0.2994 0.222 0.1649 0.1605 0.1572 0.1346 1980 0.2545 0.1783 0.113 0.1048 0.09996 0.09018 1981 0.2293 0.152 0.0872 0.07971 0.07396 0.06445 1982 0.2117 0.1349 0.07035 0.06231 0.05688 0.04789 1983 0.3093 0.192 0.09481 0.06833 0.06245 0.05455 1984 0.2066 0.1299 0.06535 0.05735 0.05199 0.04217 1985 0.1989 0.1221 0.05773 0.04973 0.04458 0.03448 1986 0.1939 0.1173 0.05293 0.04482 0.03985 0.02975 1987 0.3349 0.1979 0.07904 0.05384 0.04861 0.04078 1988 0.1898 0.1083 0.04596 0.0388 0.03368 0.02492 1989 0.1822 0.1006 0.03847 0.03142 0.02667 0.0175 1990 0.1789 0.09698 0.03512 0.02792 0.02336 0.0143

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258064516129 3.423 2.283 0. 7693 0.4279 0.3611 0.2201 0.0645161290322581 0.3349 0.222 0. 1649 0.1605 0.1572 0.1346 0.0967741935483871 0.3093 0.1979 0. 113 0.1048 0.09996 0.09018 0.129032258064516 0.2994 0.192 0. 09481 0.07971 0.07396 0.06445 0.161290322580645 0.2545 0.1783 0. 0872 0.06833 0.06245 0.05455 0.193548387096774 0.2293 0.152 0. 07904 0.06231 0.05688 0.04789 0.225806451612903 0.2117 0.1349 0. 07035 0.05735 0.05199 0.04217 0.258064516129032 0.2066 0.1299 0. 06535 0.05384 0.04861 0.04078 0.290322580645161 0.1989 0.1221 0. 0583 0.04973 0.04458 0.03448 0.32258064516129 0.198 0.1205 0. 05773 0.04658 0.04159 0.03176 0.354838709677419 0.1959 0.1184 0. 05434 0.0451 0.03985 0.02975 0.387096774193548 0.1945 0.1182 0. 05293 0.04482 0.03923 0.02958 0.419354838709677 0.1939 0.1173 0. 05078 0.04292 0.03798 0.02918 0.451612903225806 0.1926 0.1157 0. 05077 0.04278 0.03789 0.02777 0.483870967741936 0.1919 0.1146 0. 05038 0.04229 0.0374 0.02687 0.516129032258065 0.1915 0.1144 0. 05013 0.04166 0.03674 0.02672 0.548387096774194 0.1911 0.1142 0. 04934 0.04093 0.03597 0.0263

## 49 0.580645161290323 0.1905 0.1133 0. 04897 0.04088 0.03593 0.02623 0.612903225806452 0.1898 0.1132 0. 0485 0.04038 0.03565 0.0256 0.645161290322581 0.1898 0.1126 0. 04833 0.04028 0.03536 0.02558 0.67741935483871 0.1892 0.1122 0. 04821 0.04016 0.03503 0.02499 0.709677419354839 0.1885 0.1112 0. 04724 0.03928 0.03446 0.02492 0.741935483870968 0.1876 0.109 0. 04596 0.0388 0.03368 0.02434 0.774193548387097 0.1861 0.1086 0. 04497 0.03705 0.03235 0.02192 0.806451612903226 0.1855 0.1083 0. 04452 0.03636 0.03168 0.02159 0.838709677419355 0.1854 0.1082 0. 04431 0.0363 0.03154 0.02144 0.870967741935484 0.1822 0.1042 0. 04029 0.03233 0.02771 0.0175 0.903225806451613 0.1814 0.1006 0. 03847 0.03142 0.02667 0.01738 0.935483870967742 0.1789 0.09698 0. 03512 0.02792 0.02336 0.0143 0.967741935483871 0.1719 0.09507 0. 03118 0.02289 0.01846 0.007852

0.1 0.30831 0.19731 0.111181 0.102291 0.09736 0.087607 Average of yearly averages: 0.0410257333333333

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: ACAcitr1 Metfile: w23155.dvf PRZM scenario: CAcitrus_NirrigC.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.9e-5 atm-m^3/mol Vapor Pressure vapr 1.268e-6 torr Solubility sol 0.067 mg/L Kd Kd 678 mg/L Koc Koc mg/L Photolysis half-life kdp 0 days Half-life Aerobic Aquatic Metabolism kbacw 0 days Halfife Anaerobic Aquatic Metabolism kbacs 0 days Halfife Aerobic Soil Metabolism asm 0 days Halfife Hydrolysis: pH 7 0 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.34 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 15-05 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 21 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none none, mont hly or total(average of entire run)

## 50 

# CA Citrus – Model Approach 2

Stored as ACAcitr2.out Chemical: Acequinocyl PRZM environment: CAcitrus_N irrigC.txt modified Thuday , 17 June 2004 at 09:14:54 EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 17:33:30 Metfile: w23155.dvf modified Wedday, 3 July 2002 at 10:04:20 Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 0.1759 0.1281 0.04894 0.03484 0.02356 0.005942 1962 0.1763 0.1281 0.06847 0.03501 0.02376 0.01062 1963 0.1763 0.1296 0.05042 0.03567 0.02419 0.008226 1964 0.1761 0.1287 0.04946 0.03519 0.02382 0.006019 1965 0.1754 0.1278 0.04877 0.03431 0.02322 0.006137 1966 0.1755 0.1275 0.04845 0.03431 0.02322 0.007546 1967 0.1766 0.1301 0.05083 0.03612 0.02446 0.00632 1968 0.1764 0.1292 0.05007 0.03567 0.02416 0.006117 1969 0.176 0.128 0.04909 0.0349 0.02362 0.006271 1970 0.1755 0.1267 0.04767 0.03393 0.02295 0.006622 1971 0.1855 0.1342 0.06038 0.03871 0.02618 0.006636 1972 0.1749 0.1382 0.05463 0.03597 0.02433 0.00618 1973 0.1747 0.1255 0.04661 0.03288 0.02223 0.005985 1974 0.1757 0.1274 0.04829 0.03438 0.02327 0.007635 1975 0.1755 0.1269 0.04783 0.03404 0.02304 0.006001 1976 0.1751 0.1255 0.04692 0.03326 0.02248 0.005674 1977 0.1759 0.1276 0.04885 0.03466 0.02344 0.006635 1978 0.3314 0.2704 0.1168 0.04457 0.03144 0.01411 1979 0.1756 0.1278 0.04869 0.0345 0.02335 0.005972 1980 0.1768 0.131 0.0518 0.03662 0.02481 0.006374 1981 0.1764 0.1281 0.05008 0.03524 0.02384 0.006041 1982 0.176 0.1293 0.05029 0.03535 0.02392 0.006343 1983 0.1762 0.1286 0.04945 0.03524 0.02387 0.007058 1984 0.1762 0.1294 0.05019 0.03554 0.02406 0.006121 1985 0.1764 0.1291 0.05001 0.03561 0.0241 0.00612 1986 0.1763 0.1293 0.05002 0.03559 0.02409 0.00611 1987 0.1766 0.1297 0.05045 0.03599 0.02439 0.008811 1988 0.1732 0.1195 0.04149 0.02935 0.01979 0.00498 1989 0.1732 0.1188 0.0417 0.02919 0.01968 0.004959 1990 0.1727 0.1177 0.03979 0.02818 0.019 0.004785

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258064516129 0.3314 0.2704 0. 1168 0.04457 0.03144 0.01411 0.0645161290322581 0.1855 0.1382 0. 06847 0.03871 0.02618 0.01062 0.0967741935483871 0.1768 0.1342 0. 06038 0.03662 0.02481 0.008811 0.129032258064516 0.1766 0.131 0. 05463 0.03612 0.02446 0.008226 0.161290322580645 0.1766 0.1301 0. 0518 0.03599 0.02439 0.007635 0.193548387096774 0.1764 0.1297 0. 05083 0.03597 0.02433 0.007546 0.225806451612903 0.1764 0.1296 0. 05045 0.03567 0.02419 0.007058 0.258064516129032 0.1764 0.1294 0. 05042 0.03567 0.02416 0.006636 0.290322580645161 0.1763 0.1293 0. 05029 0.03561 0.0241 0.006635 0.32258064516129 0.1763 0.1293 0. 05019 0.03559 0.02409 0.006622 0.354838709677419 0.1763 0.1292 0. 05008 0.03554 0.02406 0.006374 0.387096774193548 0.1762 0.1291 0. 05007 0.03535 0.02392 0.006343 0.419354838709677 0.1762 0.1287 0. 05002 0.03524 0.02387 0.00632 0.451612903225806 0.1761 0.1286 0. 05001 0.03524 0.02384 0.006271 0.483870967741936 0.176 0.1281 0. 04946 0.03519 0.02382 0.00618 0.516129032258065 0.176 0.1281 0. 04945 0.03501 0.02376 0.006137 0.548387096774194 0.1759 0.1281 0. 04909 0.0349 0.02362 0.006121

## 51 0.580645161290323 0.1759 0.128 0. 04894 0.03484 0.02356 0.00612 0.612903225806452 0.1757 0.1278 0. 04885 0.03466 0.02344 0.006117 0.645161290322581 0.1756 0.1278 0. 04877 0.0345 0.02335 0.00611 0.67741935483871 0.1755 0.1276 0. 04869 0.03438 0.02327 0.006041 0.709677419354839 0.1755 0.1275 0. 04845 0.03431 0.02322 0.006019 0.741935483870968 0.1755 0.1274 0. 04829 0.03431 0.02322 0.006001 0.774193548387097 0.1754 0.1269 0. 04783 0.03404 0.02304 0.005985 0.806451612903226 0.1751 0.1267 0. 04767 0.03393 0.02295 0.005972 0.838709677419355 0.1749 0.1255 0. 04692 0.03326 0.02248 0.005942 0.870967741935484 0.1747 0.1255 0. 04661 0.03288 0.02223 0.005674 0.903225806451613 0.1732 0.1195 0. 0417 0.02935 0.01979 0.00498 0.935483870967742 0.1732 0.1188 0. 04149 0.02919 0.01968 0.004959 0.967741935483871 0.1727 0.1177 0. 03979 0.02818 0.019 0.004785

0.1 0.17678 0.13388 0.059805 0.03657 0.024775 0.0087525 Average of yearly averages: 0.006745

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: ACAcitr2 Metfile: w23155.dvf PRZM scenario: CAcitrus_NirrigC.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.9e-5 atm-m^3/mol Vapor Pressure vapr 1.268e-6 torr Solubility sol 0.067 mg/L Kd Kd 27 mg/L Koc Koc mg/L Photolysis half-life kdp 0.19 days Half-life Aerobic Aquatic Metabolism kbacw 97 days Halfife Anaerobic Aquatic Metabolism kbacs 649.8 days Halfife Aerobic Soil Metabolism asm 48.5 days Halfife Hydrolysis: pH 7 9.6 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.34 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 15-05 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 21 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none none, mont hly or total(average of entire run)

## 52 

# CA Grapes – Model Approach 1 stored as ACAgrps1.out Chemical: Acequinocyl PRZM environment: CAgrapes_NirrigC.txt m odified Thuday, 17 June 2004 at 09:13:38 EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 17:33:30 Metfile: w93193.dvf modified Wedday, 3 July 2002 at 10:04:24 Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 0.1721 0.09545 0.03148 0.02347 0.01897 0.009321 1962 0.1915 0.1143 0.05019 0.04244 0.03751 0.0269 1963 0.202 0.1248 0.06042 0.05284 0.04731 0.03715 1964 0.197 0.1198 0.05557 0.04774 0.0424 0.03417 1965 0.2063 0.1285 0.06433 0.05892 0.05435 0.04093 1966 0.5293 0.3412 0.141 0.07332 0.05937 0.04345 1967 0.3489 0.2309 0.1364 0.1102 0.1005 0.08018 1968 0.3704 0.2323 0.1122 0.09046 0.07847 0.06592 1969 2.488 1.47 0.6761 0.5038 0.4575 0.326 1970 0.9181 0.6265 0.3902 0.3302 0.3118 0.2545 1971 0.3439 0.2726 0.2127 0.2058 0.1999 0.167 1972 0.4543 0.3073 0.1861 0.1368 0.1272 0.1167 1973 0.4674 0.3097 0.1727 0.1411 0.1434 0.1155 1974 0.2735 0.2099 0.1336 0.114 0.1081 0.09499 1975 0.2395 0.1629 0.09773 0.09018 0.08408 0.0729 1976 1.194 0.7039 0.2952 0.2038 0.1872 0.1419 1977 0.6505 0.4061 0.2093 0.147 0.1424 0.1268 1978 0.8713 0.6639 0.3275 0.2762 0.2658 0.2024 1979 0.305 0.2272 0.1747 0.1655 0.1604 0.1337 1980 1.063 0.6472 0.3081 0.2277 0.2137 0.1598 1981 0.6848 0.4364 0.221 0.1727 0.1661 0.1278 1982 0.8764 0.549 0.2404 0.1831 0.1599 0.1304 1983 1.626 1.018 0.6573 0.4421 0.4005 0.2993 1984 0.3614 0.2839 0.2356 0.2291 0.2227 0.1852 1985 0.2988 0.2225 0.1563 0.1525 0.1489 0.1291 1986 1.718 1.019 0.3659 0.2028 0.1802 0.1541 1987 0.3456 0.2825 0.225 0.2085 0.2014 0.169 1988 0.3105 0.223 0.1645 0.1439 0.1349 0.1181 1989 0.2617 0.1843 0.1189 0.1162 0.1112 0.09447 1990 0.7757 0.4721 0.2133 0.1502 0.1412 0.1219

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258064516129 2.488 1.47 0.6761 0.5038 0.4575 0.326 0.0645161290322581 1.718 1.019 0. 6573 0.4421 0.4005 0.2993 0.0967741935483871 1.626 1.018 0. 3902 0.3302 0.3118 0.2545 0.129032258064516 1.194 0.7039 0. 3659 0.2762 0.2658 0.2024 0.161290322580645 1.063 0.6639 0. 3275 0.2291 0.2227 0.1852 0.193548387096774 0.9181 0.6472 0. 3081 0.2277 0.2137 0.169 0.225806451612903 0.8764 0.6265 0. 2952 0.2085 0.2014 0.167 0.258064516129032 0.8713 0.549 0. 2404 0.2058 0.1999 0.1598 0.290322580645161 0.7757 0.4721 0. 2356 0.2038 0.1872 0.1541 0.32258064516129 0.6848 0.4364 0. 225 0.2028 0.1802 0.1419 0.354838709677419 0.6505 0.4061 0. 221 0.1831 0.1661 0.1337 0.387096774193548 0.5293 0.3412 0. 2133 0.1727 0.1604 0.1304 0.419354838709677 0.4674 0.3097 0. 2127 0.1655 0.1599 0.1291 0.451612903225806 0.4543 0.3073 0. 2093 0.1525 0.1489 0.1278 0.483870967741936 0.3704 0.2839 0. 1861 0.1502 0.1434 0.1268 0.516129032258065 0.3614 0.2825 0. 1747 0.147 0.1424 0.1219 0.548387096774194 0.3489 0.2726 0. 1727 0.1439 0.1412 0.1181

## 53 0.580645161290323 0.3456 0.2323 0. 1645 0.1411 0.1349 0.1167 0.612903225806452 0.3439 0.2309 0. 1563 0.1368 0.1272 0.1155 0.645161290322581 0.3105 0.2272 0. 141 0.1162 0.1112 0.09499 0.67741935483871 0.305 0.223 0. 1364 0.114 0.1081 0.09447 0.709677419354839 0.2988 0.2225 0. 1336 0.1102 0.1005 0.08018 0.741935483870968 0.2735 0.2099 0. 1189 0.09046 0.08408 0.0729 0.774193548387097 0.2617 0.1843 0. 1122 0.09018 0.07847 0.06592 0.806451612903226 0.2395 0.1629 0. 09773 0.07332 0.05937 0.04345 0.838709677419355 0.2063 0.1285 0. 06433 0.05892 0.05435 0.04093 0.870967741935484 0.202 0.1248 0. 06042 0.05284 0.04731 0.03715 0.903225806451613 0.197 0.1198 0. 05557 0.04774 0.0424 0.03417 0.935483870967742 0.1915 0.1143 0. 05019 0.04244 0.03751 0.0269 0.967741935483871 0.1721 0.09545 0. 03148 0.02347 0.01897 0.009321

0.1 1.5828 0.98659 0.38777 0.3248 0.3072 0.24929 Average of yearly averages: 0.125986033333333

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: ACAgrps1 Metfile: w93193.dvf PRZM scenario: CAgrapes_NirrigC.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.9e-5 atm-m^3/mol Vapor Pressure vapr 1.268e-6 torr Solubility sol 0.067 mg/L Kd Kd 678 mg/L Koc Koc mg/L Photolysis half-life kdp 0 days Half-life Aerobic Aquatic Metabolism kbacw 0 days Halfife Anaerobic Aquatic Metabolism kbacs 0 days Halfife Aerobic Soil Metabolism asm 0 days Halfife Hydrolysis: pH 7 0 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.34 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 15-04 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 21 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none none, mont hly or total(average of entire run)

## 54 

# CA Grapes – Model Approach 2 Stored as ACAgrpe2.out Chemical: Acequinocyl PRZM environment: CAgrapes_NirrigC.txt m odified Thuday, 17 June 2004 at 09:13:38 EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 17:33:30 Metfile: w93193.dvf modified Wedday, 3 July 2002 at 10:04:24 Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 0.1771 0.1311 0.05176 0.03692 0.025 0.006778 1962 0.1769 0.1304 0.05109 0.03645 0.02469 0.006785 1963 0.1785 0.1318 0.05407 0.03786 0.02564 0.007278 1964 0.1768 0.13 0.05086 0.03623 0.02452 0.007025 1965 0.1766 0.1285 0.05062 0.03561 0.02411 0.006503 1966 0.1767 0.1312 0.0521 0.03664 0.02481 0.007812 1967 0.239 0.1859 0.09678 0.05401 0.03668 0.009722 1968 0.1767 0.1293 0.05085 0.03599 0.02436 0.007782 1969 0.1767 0.129 0.05026 0.03576 0.02506 0.009268 1970 0.1766 0.1294 0.0504 0.03588 0.02433 0.008656 1971 0.177 0.1308 0.05227 0.03713 0.02514 0.006593 1972 0.1764 0.1295 0.0503 0.03577 0.02422 0.007222 1973 0.1767 0.1306 0.05134 0.0364 0.02466 0.006584 1974 0.1768 0.1291 0.05086 0.03593 0.02432 0.00727 1975 0.1779 0.1316 0.05342 0.0377 0.02555 0.006635 1976 0.1778 0.1316 0.05296 0.03755 0.02544 0.0106 1977 0.1763 0.1295 0.05041 0.03565 0.02414 0.006685 1978 0.1778 0.1299 0.05285 0.03675 0.02488 0.007523 1979 0.1758 0.1279 0.04877 0.03468 0.02346 0.006148 1980 0.1766 0.1287 0.05033 0.03559 0.02409 0.007025 1981 0.1765 0.1279 0.05078 0.03541 0.02395 0.006551 1982 0.1768 0.1298 0.05098 0.03623 0.02452 0.009136 1983 0.2378 0.1746 0.09675 0.0536 0.03642 0.01129 1984 0.176 0.1269 0.04915 0.03453 0.02336 0.006312 1985 0.1766 0.1292 0.0506 0.03585 0.02427 0.006459 1986 0.1767 0.1296 0.05064 0.03602 0.02439 0.008123 1987 0.177 0.1308 0.05213 0.03688 0.02501 0.007163 1988 0.1817 0.1351 0.06759 0.04329 0.02935 0.00762 1989 0.1761 0.1282 0.04931 0.03504 0.02371 0.006304 1990 0.2083 0.154 0.05975 0.05567 0.03878 0.01038

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258064516129 0.239 0.1859 0. 09678 0.05567 0.03878 0.01129 0.0645161290322581 0.2378 0.1746 0. 09675 0.05401 0.03668 0.0106 0.0967741935483871 0.2083 0.154 0. 06759 0.0536 0.03642 0.01038 0.129032258064516 0.1817 0.1351 0. 05975 0.04329 0.02935 0.009722 0.161290322580645 0.1785 0.1318 0. 05407 0.03786 0.02564 0.009268 0.193548387096774 0.1779 0.1316 0. 05342 0.0377 0.02555 0.009136 0.225806451612903 0.1778 0.1316 0. 05296 0.03755 0.02544 0.008656 0.258064516129032 0.1778 0.1312 0. 05285 0.03713 0.02514 0.008123 0.290322580645161 0.1771 0.1311 0. 05227 0.03692 0.02506 0.007812 0.32258064516129 0.177 0.1308 0. 05213 0.03688 0.02501 0.007782 0.354838709677419 0.177 0.1308 0. 0521 0.03675 0.025 0.00762 0.387096774193548 0.1769 0.1306 0. 05176 0.03664 0.02488 0.007523 0.419354838709677 0.1768 0.1304 0. 05134 0.03645 0.02481 0.007278 0.451612903225806 0.1768 0.13 0. 05109 0.0364 0.02469 0.00727 0.483870967741936 0.1768 0.1299 0. 05098 0.03623 0.02466 0.007222 0.516129032258065 0.1767 0.1298 0. 05086 0.03623 0.02452 0.007163 0.548387096774194 0.1767 0.1296 0. 05086 0.03602 0.02452 0.007025

## 55 0.580645161290323 0.1767 0.1295 0. 05085 0.03599 0.02439 0.007025 0.612903225806452 0.1767 0.1295 0. 05078 0.03593 0.02436 0.006785 0.645161290322581 0.1767 0.1294 0. 05064 0.03588 0.02433 0.006778 0.67741935483871 0.1766 0.1293 0. 05062 0.03585 0.02432 0.006685 0.709677419354839 0.1766 0.1292 0. 0506 0.03577 0.02427 0.006635 0.741935483870968 0.1766 0.1291 0. 05041 0.03576 0.02422 0.006593 0.774193548387097 0.1766 0.129 0. 0504 0.03565 0.02414 0.006584 0.806451612903226 0.1765 0.1287 0. 05033 0.03561 0.02411 0.006551 0.838709677419355 0.1764 0.1285 0. 0503 0.03559 0.02409 0.006503 0.870967741935484 0.1763 0.1282 0. 05026 0.03541 0.02395 0.006459 0.903225806451613 0.1761 0.1279 0. 04931 0.03504 0.02371 0.006312 0.935483870967742 0.176 0.1279 0. 04915 0.03468 0.02346 0.006304 0.967741935483871 0.1758 0.1269 0. 04877 0.03453 0.02336 0.006148

0.1 0.20564 0.15211 0.066806 0.052569 0.035713 0.0103142 Average of yearly averages: 0.00764106666666667

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: ACAgrpe2 Metfile: w93193.dvf PRZM scenario: CAgrapes_NirrigC.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.9e-5 atm-m^3/mol Vapor Pressure vapr 1.268e-6 torr Solubility sol 0.067 mg/L Kd Kd 27 mg/L Koc Koc mg/L Photolysis half-life kdp 0.19 days Half-life Aerobic Aquatic Metabolism kbacw 97 days Halfife Anaerobic Aquatic Metabolism kbacs 649.8 days Halfife Aerobic Soil Metabolism asm 48.5 days Halfife Hydrolysis: pH 7 9.6 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.34 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 15-04 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 21 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none none, mont hly or total(average of entire run)

## 56 

# FL Citrus – Mo del Approach 1

stored as AFLcitr1.out Chemical: Acequinocyl PRZM environment: FLcitr usC.txt modified Satday, 12 October 2002 at 16:39:50 EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 17:33:30 Metfile: w12842.dvf modified Wedday, 3 July 2002 at 10:04:28 Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 0.2054 0.1215 0.05394 0.04719 0.04251 0.0246 1962 0.7857 0.4683 0.2051 0.1635 0.1463 0.08327 1963 0.9674 0.5856 0.2701 0.2056 0.1928 0.1509 1964 1.71 1.028 0.5079 0.4413 0.4009 0.2811 1965 1.217 0.8142 0.4875 0.4006 0.3746 0.3024 1966 1.205 0.7837 0.4337 0.366 0.3501 0.2916 1967 2.008 1.359 0.6491 0.4652 0.4295 0.3055 1968 1.207 0.9174 0.5143 0.4447 0.4324 0.3473 1969 1.594 1.06 0.7259 0.622 0.5899 0.4629 1970 1.076 0.7759 0.5423 0.4687 0.4684 0.4227 1971 2.273 1.575 0.8068 0.6447 0.598 0.4426 1972 2.08 1.329 0.813 0.6253 0.5919 0.4818 1973 1.576 1.021 0.6296 0.5986 0.5471 0.4617 1974 4.751 2.745 1.174 0.7646 0.6977 0.4845 1975 1.918 1.208 0.7338 0.6671 0.6198 0.493 1976 2.965 1.796 0.8259 0.7082 0.6561 0.5369 1977 0.7854 0.5883 0.4937 0.4683 0.4564 0.4069 1978 1.008 0.6966 0.4418 0.3889 0.3754 0.3529 1979 10.68 6.015 2.165 1.27 1.135 0.7822 1980 1.386 1.042 0.789 0.7443 0.7435 0.6932 1981 2.046 1.34 0.7969 0.7289 0.6761 0.6047 1982 2.606 1.705 1.028 0.8819 0.8945 0.6778 1983 2.558 1.647 0.9407 0.8354 0.8303 0.7197 1984 1.439 1.009 0.6732 0.6229 0.6107 0.5685 1985 3.676 2.233 1.068 0.7807 0.7511 0.5567 1986 1.681 1.244 0.7189 0.6122 0.5894 0.5614 1987 2.311 1.613 0.8695 0.6868 0.6468 0.5855 1988 3.182 2.314 1.212 0.9333 0.8399 0.6075 1989 1.248 0.995 0.8179 0.7169 0.6903 0.6093 1990 2.206 1.42 0.8319 0.6429 0.6006 0.5242

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258064516129 10.68 6.015 2.165 1.27 1.135 0.7822 0.0645161290322581 4.751 2.745 1. 212 0.9333 0.8945 0.7197 0.0967741935483871 3.676 2.314 1. 174 0.8819 0.8399 0.6932 0.129032258064516 3.182 2.233 1. 068 0.8354 0.8303 0.6778 0.161290322580645 2.965 1.796 1. 028 0.7807 0.7511 0.6093 0.193548387096774 2.606 1.705 0. 9407 0.7646 0.7435 0.6075 0.225806451612903 2.558 1.647 0. 8695 0.7443 0.6977 0.6047 0.258064516129032 2.311 1.613 0. 8319 0.7289 0.6903 0.5855 0.290322580645161 2.273 1.575 0. 8259 0.7169 0.6761 0.5685 0.32258064516129 2.206 1.42 0.8179 0.7082 0.6561 0.5614 0.354838709677419 2.08 1.359 0.813 0.6868 0.6468 0.5567 0.387096774193548 2.046 1.34 0. 8068 0.6671 0.6198 0.5369 0.419354838709677 2.008 1.329 0. 7969 0.6447 0.6107 0.5242 0.451612903225806 1.918 1.244 0.789 0.6429 0.6006 0.493 0.483870967741936 1.71 1.208 0.7338 0.6253 0.598 0.4845 0.516129032258065 1.681 1.06 0. 7259 0.6229 0.5919 0.4818 0.548387096774194 1.594 1.042 0. 7189 0.622 0.5899 0.4629

## 57 0.580645161290323 1.576 1.028 0. 6732 0.6122 0.5894 0.4617 0.612903225806452 1.439 1.021 0. 6491 0.5986 0.5471 0.4426 0.645161290322581 1.386 1.009 0. 6296 0.4687 0.4684 0.4227 0.67741935483871 1.248 0.995 0. 5423 0.4683 0.4564 0.4069 0.709677419354839 1.217 0.9174 0. 5143 0.4652 0.4324 0.3529 0.741935483870968 1.207 0.8142 0. 5079 0.4447 0.4295 0.3473 0.774193548387097 1.205 0.7837 0. 4937 0.4413 0.4009 0.3055 0.806451612903226 1.076 0.7759 0. 4875 0.4006 0.3754 0.3024 0.838709677419355 1.008 0.6966 0. 4418 0.3889 0.3746 0.2916 0.870967741935484 0.9674 0.5883 0. 4337 0.366 0.3501 0.2811 0.903225806451613 0.7857 0.5856 0. 2701 0.2056 0.1928 0.1509 0.935483870967742 0.7854 0.4683 0. 2051 0.1635 0.1463 0.08327 0.967741935483871 0.2054 0.1215 0. 05394 0.04719 0.04251 0.0246

0.1 3.6266 2.3059 1.1634 0.87725 0.83894 0.69166 Average of yearly averages: 0.460775666666667

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: AFLcitr1 Metfile: w12842.dvf PRZM scenario: FLcitrusC.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.9e-5 atm-m^3/mol Vapor Pressure vapr 1.268e-6 torr Solubility sol 0.067 mg/L Kd Kd 678 mg/L Koc Koc mg/L Photolysis half-life kdp 0 days Half-life Aerobic Aquatic Metabolism kbacw 0 days Halfife Anaerobic Aquatic Metabolism kbacs 0 days Halfife Aerobic Soil Metabolism asm 0 days Halfife Hydrolysis: pH 7 0 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.34 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 15-3 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 21 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none none, mont hly or total(average of entire run)

## 58 

# FL Citrus – Mo del Approach 2

Stored as AFLcitr2.out Chemical: Acequinocyl PRZM environment: FLcitr usC.txt modified Satday, 12 October 2002 at 16:39:50 EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 17:33:30 Metfile: w12842.dvf modified Wedday, 3 July 2002 at 10:04:28 Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 0.2835 0.2047 0.1053 0.06295 0.05021 0.02042 1962 0.4821 0.3803 0.2192 0.1153 0.08385 0.03191 1963 0.3962 0.2868 0.1816 0.1008 0.07132 0.02515 1964 1.26 0.9129 0.3586 0.2323 0.1695 0.06558 1965 0.6814 0.5318 0.2683 0.1432 0.1067 0.03428 1966 0.9799 0.7467 0.2999 0.1513 0.1085 0.03696 1967 0.3567 0.2683 0.1466 0.1094 0.09536 0.03128 1968 0.6749 0.494 0.1932 0.1421 0.1036 0.03626 1969 1.4 1.023 0.4717 0.2253 0.2084 0.06898 1970 0.684 0.4973 0.2444 0.09649 0.09914 0.03262 1971 2.013 1.447 0.5383 0.2441 0.179 0.05715 1972 0.721 0.5151 0.1897 0.1097 0.09552 0.03592 1973 1.21 0.8812 0.3784 0.1617 0.1099 0.03917 1974 1.748 1.285 0.547 0.2366 0.1631 0.0544 1975 0.742 0.545 0.2871 0.1612 0.1138 0.04029 1976 2.102 1.557 0.6271 0.2967 0.2132 0.06295 1977 0.176 0.1283 0.04916 0.03496 0.02774 0.01378 1978 0.3113 0.2288 0.1261 0.08974 0.07522 0.024 1979 9.416 6.983 2.819 1.082 0.749 0.2003 1980 1.26 0.9233 0.4257 0.2252 0.1778 0.05366 1981 0.781 0.5766 0.2896 0.1369 0.1039 0.03907 1982 0.7168 0.5534 0.3269 0.2207 0.1589 0.05206 1983 1.229 0.902 0.399 0.1948 0.1982 0.06596 1984 0.8005 0.5859 0.2259 0.1062 0.08583 0.03048 1985 0.8638 0.6434 0.2846 0.1412 0.1124 0.04096 1986 1.455 1.19 0.4771 0.2111 0.1486 0.04644 1987 1.737 1.33 0.6336 0.3012 0.2243 0.068 1988 0.324 0.2377 0.1039 0.07195 0.06596 0.03436 1989 0.396 0.2935 0.1503 0.08722 0.06787 0.02398 1990 0.4237 0.3155 0.1552 0.08662 0.06414 0.02671

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258064516129 9.416 6.983 2.819 1.082 0.749 0.2003 0.0645161290322581 2.102 1.557 0. 6336 0.3012 0.2243 0.06898 0.0967741935483871 2.013 1.447 0. 6271 0.2967 0.2132 0.068 0.129032258064516 1.748 1.33 0. 547 0.2441 0.2084 0.06596 0.161290322580645 1.737 1.285 0. 5383 0.2366 0.1982 0.06558 0.193548387096774 1.455 1.19 0. 4771 0.2323 0.179 0.06295 0.225806451612903 1.4 1.023 0.4717 0.2253 0.1778 0.05715 0.258064516129032 1.26 0.9233 0. 4257 0.2252 0.1695 0.0544 0.290322580645161 1.26 0.9129 0. 399 0.2207 0.1631 0.05366 0.32258064516129 1.229 0.902 0. 3784 0.2111 0.1589 0.05206 0.354838709677419 1.21 0.8812 0. 3586 0.1948 0.1486 0.04644 0.387096774193548 0.9799 0.7467 0. 3269 0.1617 0.1138 0.04096 0.419354838709677 0.8638 0.6434 0. 2999 0.1612 0.1124 0.04029 0.451612903225806 0.8005 0.5859 0. 2896 0.1513 0.1099 0.03917 0.483870967741936 0.781 0.5766 0. 2871 0.1432 0.1085 0.03907 0.516129032258065 0.742 0.5534 0. 2846 0.1421 0.1067 0.03696 0.548387096774194 0.721 0.545 0. 2683 0.1412 0.1039 0.03626

## 59 0.580645161290323 0.7168 0.5318 0. 2444 0.1369 0.1036 0.03592 0.612903225806452 0.684 0.5151 0. 2259 0.1153 0.09914 0.03436 0.645161290322581 0.6814 0.4973 0. 2192 0.1097 0.09552 0.03428 0.67741935483871 0.6749 0.494 0. 1932 0.1094 0.09536 0.03262 0.709677419354839 0.4821 0.3803 0. 1897 0.1062 0.08583 0.03191 0.741935483870968 0.4237 0.3155 0. 1816 0.1008 0.08385 0.03128 0.774193548387097 0.3962 0.2935 0. 1552 0.09649 0.07522 0.03048 0.806451612903226 0.396 0.2868 0. 1503 0.08974 0.07132 0.02671 0.838709677419355 0.3567 0.2683 0. 1466 0.08722 0.06787 0.02515 0.870967741935484 0.324 0.2377 0. 1261 0.08662 0.06596 0.024 0.903225806451613 0.3113 0.2288 0. 1053 0.07195 0.06414 0.02398 0.935483870967742 0.2835 0.2047 0. 1039 0.06295 0.05021 0.02042 0.967741935483871 0.176 0.1283 0. 04916 0.03496 0.02774 0.01378

0.1 1.9865 1.4353 0.61909 0.29144 0.21272 0.067796 Average of yearly averages: 0.046436

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: AFLcitr2 Metfile: w12842.dvf PRZM scenario: FLcitrusC.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.9e-5 atm-m^3/mol Vapor Pressure vapr 1.268e-6 torr Solubility sol 0.067 mg/L Kd Kd 27 mg/L Koc Koc mg/L Photolysis half-life kdp 0.19 days Half-life Aerobic Aquatic Metabolism kbacw 97 days Halfife Anaerobic Aquatic Metabolism kbacs 649.8 days Halfife Aerobic Soil Metabolism asm 48.5 days Halfife Hydrolysis: pH 7 9.6 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.34 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 15-03 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 21 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none none, mont hly or total(average of entire run)

## 60 

# GA Pecans – Model Approach 1

stored as AGAPcan1.out Chemical: Acequinocyl PRZM environment: GAPecansC.txt modified Tueday, 22 April 2003 at 07:57:20 EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 17:33:30 Metfile: w93805.dvf modified Wedday, 3 July 2002 at 10:04:32 Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 1.364 0.9333 0.3628 0.2279 0.2027 0.07797 1962 1.533 0.9352 0.4314 0.3652 0.3393 0.2394 1963 2.138 1.311 0.6455 0.5209 0.5369 0.3949 1964 3.806 2.334 1.312 1.042 0.9975 0.7271 1965 2.48 1.723 1.089 1.023 1.004 0.8675 1966 2.395 1.815 1.236 1.101 1.058 0.9096 1967 1.752 1.33 0.9909 0.9232 0.8954 0.857 1968 4.836 3.003 1.425 1.126 1.096 0.8341 1969 6.399 4.008 1.996 1.483 1.403 1.086 1970 7.29 4.545 2.168 1.676 1.559 1.264 1971 3.394 2.339 1.669 1.361 1.293 1.133 1972 2.796 1.923 1.281 1.09 1.067 1.026 1973 2.94 2.107 1.545 1.284 1.231 1.139 1974 3.497 2.422 1.525 1.365 1.299 1.094 1975 5.737 3.988 2.086 1.635 1.541 1.274 1976 3.672 2.749 1.62 1.454 1.375 1.211 1977 2.843 1.997 1.451 1.248 1.188 1.103 1978 2.961 2.018 1.278 1.127 1.064 0.971 1979 2.897 2.234 1.588 1.322 1.28 1.095 1980 4.788 3.345 1.783 1.407 1.33 1.147 1981 2.971 2.086 1.328 1.232 1.176 1.044 1982 3.267 2.184 1.298 1.197 1.167 0.9851 1983 2.739 1.939 1.242 1.084 1.038 0.95 1984 1.826 1.386 1.16 1.043 0.9925 0.9223 1985 3.683 2.491 1.398 1.319 1.168 0.9186 1986 2.498 1.846 1.415 1.343 1.283 1.145 1987 3.147 2.356 1.58 1.324 1.291 1.184 1988 2.62 1.973 1.481 1.236 1.189 1.071 1989 2.338 1.692 1.321 1.145 1.093 0.9831 1990 1.785 1.323 0.9916 0.9396 0.9236 0.867

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258064516129 7.29 4.545 2.168 1.676 1.559 1.274 0.0645161290322581 6.399 4.008 2.086 1.635 1.541 1.264 0.0967741935483871 5.737 3.988 1.996 1.483 1.403 1.211 0.129032258064516 4.836 3.345 1.783 1.454 1.375 1.184 0.161290322580645 4.788 3.003 1.669 1.407 1.33 1.147 0.193548387096774 3.806 2.749 1.62 1.365 1.299 1.145 0.225806451612903 3.683 2.491 1.588 1.361 1.293 1.139 0.258064516129032 3.672 2.422 1.58 1.343 1.291 1.133 0.290322580645161 3.497 2.356 1.545 1.324 1.283 1.103 0.32258064516129 3.394 2.339 1.525 1.322 1.28 1.095 0.354838709677419 3.267 2.334 1.481 1.319 1.231 1.094 0.387096774193548 3.147 2.234 1.451 1.284 1.189 1.086 0.419354838709677 2.971 2.184 1.425 1.248 1.188 1.071 0.451612903225806 2.961 2.107 1.415 1.236 1.176 1.044 0.483870967741936 2.94 2.086 1.398 1.232 1.168 1.026 0.516129032258065 2.897 2.018 1.328 1.197 1.167 0.9851 0.548387096774194 2.843 1.997 1.321 1.145 1.096 0.9831

## 61 0.580645161290323 2.796 1.973 1.312 1.127 1.093 0.971 0.612903225806452 2.739 1.939 1.298 1.126 1.067 0.95 0.645161290322581 2.62 1.923 1.281 1.101 1.064 0.9223 0.67741935483871 2.498 1.846 1.278 1.09 1.058 0.9186 0.709677419354839 2.48 1.815 1.242 1.084 1.038 0.9096 0.741935483870968 2.395 1.723 1.236 1.043 1.004 0.8675 0.774193548387097 2.338 1.692 1.16 1.042 0.9975 0.867 0.806451612903226 2.138 1.386 1.089 1.023 0.9925 0.857 0.838709677419355 1.826 1.33 0. 9916 0.9396 0.9236 0.8341 0.870967741935484 1.785 1.323 0. 9909 0.9232 0.8954 0.7271 0.903225806451613 1.752 1.311 0. 6455 0.5209 0.5369 0.3949 0.935483870967742 1.533 0.9352 0. 4314 0.3652 0.3393 0.2394 0.967741935483871 1.364 0.9333 0. 3628 0.2279 0.2027 0.07797

0.1 5.6469 3.9237 1.9747 1.4801 1.4002 1.2083 Average of yearly averages: 0.950689

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: AGAPcan1 Metfile: w93805.dvf PRZM scenario: GAPecansC.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.9e-5 atm-m^3/mol Vapor Pressure vapr 1.268e-6 torr Solubility sol 0.067 mg/L Kd Kd 678 mg/L Koc Koc mg/L Photolysis half-life kdp 0 days Half-life Aerobic Aquatic Metabolism kbacw 0 days Halfife Anaerobic Aquatic Metabolism kbacs 0 days Halfife Aerobic Soil Metabolism asm 0 days Halfife Hydrolysis: pH 7 0 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.34 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 01-07 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 21 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none none, mont hly or total(average of entire run)

## 62 

# GA Pecans – Model Approach 2 stored as AGApcan2.out Chemical: Acequinocyl PRZM environment: GAPecansC.txt modified Tueday, 22 April 2003 at 07:57:20 EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 17:33:30 Metfile: w93805.dvf modified Wedday, 3 July 2002 at 10:04:32 Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 1.021 0.811 0.3779 0.2704 0.1971 0.05223 1962 1.491 1.109 0.4726 0.234 0.1815 0.05311 1963 2.732 2.036 0.8625 0.3988 0.3122 0.08678 1964 3.666 2.752 1.705 0.9447 0.683 0.1896 1965 2.381 1.826 0.8439 0.4792 0.3716 0.1044 1966 1.6 1.25 0.5991 0.3505 0.321 0.08983 1967 0.722 0.6103 0.2754 0.2288 0.1866 0.05711 1968 1.803 1.364 0.5747 0.2607 0.1952 0.06211 1969 2.235 1.702 1.032 0.4741 0.483 0.1324 1970 9.357 7.121 3.017 1.289 0.9103 0.2413 1971 2.682 2.046 1.2 0.5383 0.3803 0.1055 1972 0.5513 0.415 0.1733 0.1013 0.1027 0.0391 1973 1.099 0.9095 0.4981 0.243 0.1778 0.0513 1974 1.718 1.323 0.856 0.4872 0.3504 0.09306 1975 5.409 4.103 1.909 0.8785 0.6205 0.1662 1976 1.022 0.7667 0.3348 0.1901 0.1955 0.07121 1977 2.08 1.567 0.8273 0.3856 0.2726 0.0741 1978 1.977 1.5 0.7488 0.381 0.2673 0.07263 1979 2.26 1.743 1.244 0.6378 0.4642 0.129 1980 5.201 3.948 1.786 0.7166 0.5027 0.1376 1981 0.5434 0.4194 0.1841 0.1011 0.08863 0.02878 1982 0.6237 0.4864 0.3748 0.2726 0.2163 0.05895 1983 0.2756 0.2142 0.1024 0.05344 0.05327 0.02337 1984 0.8443 0.6453 0.3087 0.2407 0.1822 0.04973 1985 0.9989 0.764 0.3899 0.2897 0.2183 0.06993 1986 1.962 1.495 0.7277 0.4845 0.3631 0.1001 1987 1.615 1.268 0.6219 0.346 0.2544 0.07171 1988 0.4164 0.3155 0.2218 0.1432 0.1132 0.03667 1989 0.9453 0.7211 0.3329 0.1734 0.1343 0.04499 1990 0.727 0.6118 0.2884 0.1647 0.1296 0.03766

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258064516129 9.357 7.121 3.017 1.289 0.9103 0.2413 0.0645161290322581 5.409 4.103 1. 909 0.9447 0.683 0.1896 0.0967741935483871 5.201 3.948 1. 786 0.8785 0.6205 0.1662 0.129032258064516 3.666 2.752 1. 705 0.7166 0.5027 0.1376 0.161290322580645 2.732 2.046 1.244 0.6378 0.483 0.1324 0.193548387096774 2.682 2.036 1.2 0.5383 0.4642 0.129 0.225806451612903 2.381 1.826 1. 032 0.4872 0.3803 0.1055 0.258064516129032 2.26 1.743 0. 8625 0.4845 0.3716 0.1044 0.290322580645161 2.235 1.702 0. 856 0.4792 0.3631 0.1001 0.32258064516129 2.08 1.567 0. 8439 0.4741 0.3504 0.09306 0.354838709677419 1.977 1.5 0.8273 0.3988 0.321 0.08983 0.387096774193548 1.962 1.495 0. 7488 0.3856 0.3122 0.08678 0.419354838709677 1.803 1.364 0. 7277 0.381 0.2726 0.0741 0.451612903225806 1.718 1.323 0. 6219 0.3505 0.2673 0.07263 0.483870967741936 1.615 1.268 0. 5991 0.346 0.2544 0.07171 0.516129032258065 1.6 1.25 0.5747 0.2897 0.2183 0.07121 0.548387096774194 1.491 1.109 0. 4981 0.2726 0.2163 0.06993

## 63 0.580645161290323 1.099 0.9095 0. 4726 0.2704 0.1971 0.06211 0.612903225806452 1.022 0.811 0. 3899 0.2607 0.1955 0.05895 0.645161290322581 1.021 0.7667 0. 3779 0.243 0.1952 0.05711 0.67741935483871 0.9989 0.764 0. 3748 0.2407 0.1866 0.05311 0.709677419354839 0.9453 0.7211 0. 3348 0.234 0.1822 0.05223 0.741935483870968 0.8443 0.6453 0. 3329 0.2288 0.1815 0.0513 0.774193548387097 0.727 0.6118 0. 3087 0.1901 0.1778 0.04973 0.806451612903226 0.722 0.6103 0. 2884 0.1734 0.1343 0.04499 0.838709677419355 0.6237 0.4864 0. 2754 0.1647 0.1296 0.0391 0.870967741935484 0.5513 0.4194 0. 2218 0.1432 0.1132 0.03766 0.903225806451613 0.5434 0.415 0. 1841 0.1013 0.1027 0.03667 0.935483870967742 0.4164 0.3155 0. 1733 0.1011 0.08863 0.02878 0.967741935483871 0.2756 0.2142 0. 1024 0.05344 0.05327 0.02337

0.1 5.0475 3.8284 1.7779 0.86231 0.60872 0.16334 Average of yearly averages: 0.0843486666666666

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: AGApcan2 Metfile: w93805.dvf PRZM scenario: GAPecansC.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.9e-5 atm-m^3/mol Vapor Pressure vapr 1.268e-6 torr Solubility sol 0.067 mg/L Kd Kd 27 mg/L Koc Koc mg/L Photolysis half-life kdp 0.19 days Half-life Aerobic Aquatic Metabolism kbacw 97 days Halfife Anaerobic Aquatic Metabolism kbacs 649.8 days Halfife Aerobic Soil Metabolism asm 48.5 days Halfife Hydrolysis: pH 7 9.6 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.34 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 01-07 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 21 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none none, mont hly or total(average of entire run)

## 64 

# NC Apples – Model Approach 1 stored as ANCappl1.out Chemical: Acequinocyl PRZM environment: NCappl eC.txt modified Satday, 12 October 2002 at 17:09:36 EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 17:33:30 Metfile: w03812.dvf modified Wedday, 3 July 2002 at 10:05:50 Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1965 0.2979 0.1717 0.1106 0.07736 0.07029 0.02977 1966 1.579 0.9353 0.4324 0.3587 0.3429 0.1923 1967 2.958 2.15 1.061 0.7439 0.6749 0.4093 1968 2.045 1.357 0.8499 0.6789 0.653 0.5474 1969 3.676 2.24 1.011 0.7819 0.7285 0.5398 1970 1.34 0.951 0.6135 0.5709 0.5465 0.4883 1971 1.188 0.8258 0.5591 0.4936 0.4657 0.4211 1972 1.827 1.158 0.6271 0.5646 0.5299 0.4625 1973 2.609 1.742 1.006 0.8932 0.8378 0.6699 1974 1.849 1.286 0.8284 0.6991 0.6715 0.6328 1975 2.58 1.645 0.8829 0.7637 0.7106 0.5993 1976 1.78 1.322 0.8074 0.7404 0.7281 0.6553 1977 2.341 1.61 1.116 0.9144 0.9105 0.699 1978 2.452 1.663 1.102 0.9309 0.8818 0.7834 1979 2.444 1.616 0.9343 0.8134 0.7787 0.7313 1980 1.202 0.965 0.7929 0.7038 0.6982 0.636 1981 1.812 1.21 0.8849 0.6962 0.6506 0.5413 1982 2.179 1.524 1.06 0.8156 0.7627 0.6369 1983 1.623 1.169 0.8989 0.7138 0.6712 0.6217 1984 1.72 1.207 0.8598 0.8003 0.7618 0.6939 1985 3.967 2.433 1.135 0.8331 0.7728 0.6378 1986 2.879 1.804 0.9494 0.7341 0.7163 0.5912 1987 2.904 1.857 1.097 0.8586 0.8127 0.7102 1988 1.049 0.9415 0.7987 0.7126 0.6776 0.5679 1989 2.448 1.605 1.012 0.8731 0.8612 0.653 1990 4.632 2.955 1.467 1.175 1.123 0.894

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.037037037037037 4.632 2.955 1.467 1.175 1.123 0.894 0.0740740740740741 3.967 2.433 1. 135 0.9309 0.9105 0.7834 0.111111111111111 3.676 2.24 1.116 0.9144 0.8818 0.7313 0.148148148148148 2.958 2.15 1.102 0.8932 0.8612 0.7102 0.185185185185185 2.904 1.857 1.097 0.8731 0.8378 0.699 0.222222222222222 2.879 1.804 1. 061 0.8586 0.8127 0.6939 0.259259259259259 2.609 1.742 1.06 0.8331 0.7787 0.6699 0.296296296296296 2.58 1.663 1.012 0.8156 0.7728 0.6553 0.333333333333333 2.452 1.645 1.011 0.8134 0.7627 0.653 0.37037037037037 2.448 1.616 1.006 0.8003 0.7618 0.6378 0.407407407407407 2.444 1.61 0. 9494 0.7819 0.7285 0.6369 0.444444444444444 2.341 1.605 0. 9343 0.7637 0.7281 0.636 0.481481481481481 2.179 1.524 0. 8989 0.7439 0.7163 0.6328 0.518518518518518 2.045 1.357 0. 8849 0.7404 0.7106 0.6217 0.555555555555556 1.849 1.322 0. 8829 0.7341 0.6982 0.5993 0.592592592592593 1.827 1.286 0. 8598 0.7138 0.6776 0.5912 0.62962962962963 1.812 1.21 0.8499 0.7126 0.6749 0.5679 0.666666666666667 1.78 1.207 0. 8284 0.7038 0.6715 0.5474 0.703703703703704 1.72 1.169 0. 8074 0.6991 0.6712 0.5413 0.740740740740741 1.623 1.158 0. 7987 0.6962 0.653 0.5398 0.777777777777778 1.579 0.965 0. 7929 0.6789 0.6506 0.4883

## 65 0.814814814814815 1.34 0.951 0. 6271 0.5709 0.5465 0.4625 0.851851851851852 1.202 0.9415 0. 6135 0.5646 0.5299 0.4211 0.888888888888889 1.188 0.9353 0. 5591 0.4936 0.4657 0.4093 0.925925925925926 1.049 0.8258 0. 4324 0.3587 0.3429 0.1923 0.962962962962963 0.2979 0.1717 0. 1106 0.07736 0.07029 0.02977

0.1 3.7633 2.2979 1.1217 0.91935 0.89041 0.74693 Average of yearly averages: 0.578668076923077

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: ANCappl1 Metfile: w03812.dvf PRZM scenario: NCappleC.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.9e-5 atm-m^3/mol Vapor Pressure vapr 1.268e-6 torr Solubility sol 0.067 mg/L Kd Kd 678 mg/L Koc Koc mg/L Photolysis half-life kdp 0 days Half-life Aerobic Aquatic Metabolism kbacw 0 days Halfife Anaerobic Aquatic Metabolism kbacs 0 days Halfife Aerobic Soil Metabolism asm 0 days Halfife Hydrolysis: pH 7 0 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.34 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 01-07 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 21 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none none, mont hly or total(average of entire run)

## 66 

# NC Apples – Model Approach 2 Stored as ANCAppl2.out Chemical: Acequinocyl PRZM environment: NCappl eC.txt modified Satday, 12 October 2002 at 17:09:36 EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 17:33:30 Metfile: w03812.dvf modified Wedday, 3 July 2002 at 10:05:50 Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1965 0.6363 0.483 0.2203 0.1175 0.1159 0.03323 1966 2.31 1.755 0.8289 0.4059 0.297 0.08589 1967 3.049 2.486 1.226 0.6032 0.4764 0.1286 1968 2.625 2.022 0.9978 0.4552 0.3231 0.09215 1969 4.829 3.662 1.552 0.6733 0.4765 0.129 1970 0.6798 0.5623 0.3214 0.153 0.1068 0.03941 1971 0.6638 0.512 0.2495 0.1334 0.09979 0.03677 1972 0.6564 0.4974 0.2623 0.1423 0.1319 0.04295 1973 1.989 1.523 0.8468 0.5537 0.39 0.1067 1974 1.51 1.156 0.5461 0.2688 0.1946 0.0554 1975 1.023 0.7693 0.3348 0.1652 0.127 0.04306 1976 0.6053 0.461 0.2115 0.1694 0.1461 0.04719 1977 1.234 0.9375 0.6829 0.3378 0.2673 0.08231 1978 2.322 1.77 0.8005 0.3612 0.2612 0.07736 1979 0.4489 0.3427 0.1935 0.1228 0.1105 0.044 1980 0.2889 0.2248 0.09501 0.05799 0.05332 0.01879 1981 0.1884 0.1433 0.06158 0.04207 0.02871 0.01104 1982 1.419 1.085 0.7985 0.388 0.2711 0.0845 1983 0.5025 0.3831 0.211 0.1135 0.08628 0.03532 1984 0.8829 0.6763 0.4035 0.265 0.1931 0.05514 1985 2.183 1.66 0.7121 0.3118 0.227 0.06339 1986 2.106 1.606 0.7278 0.3155 0.2354 0.07195 1987 1.18 0.8936 0.5191 0.2039 0.1521 0.04646 1988 0.2854 0.2171 0.09265 0.05922 0.06081 0.02247 1989 1.899 1.435 0.7073 0.4223 0.325 0.09473 1990 3.024 2.355 1.033 0.4951 0.3701 0.1133

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.037037037037037 4.829 3.662 1.552 0.6733 0.4765 0.129 0.0740740740740741 3.049 2.486 1. 226 0.6032 0.4764 0.1286 0.111111111111111 3.024 2.355 1.033 0.5537 0.39 0.1133 0.148148148148148 2.625 2.022 0. 9978 0.4951 0.3701 0.1067 0.185185185185185 2.322 1.77 0. 8468 0.4552 0.325 0.09473 0.222222222222222 2.31 1.755 0. 8289 0.4223 0.3231 0.09215 0.259259259259259 2.183 1.66 0. 8005 0.4059 0.297 0.08589 0.296296296296296 2.106 1.606 0. 7985 0.388 0.2711 0.0845 0.333333333333333 1.989 1.523 0. 7278 0.3612 0.2673 0.08231 0.37037037037037 1.899 1.435 0. 7121 0.3378 0.2612 0.07736 0.407407407407407 1.51 1.156 0. 7073 0.3155 0.2354 0.07195 0.444444444444444 1.419 1.085 0. 6829 0.3118 0.227 0.06339 0.481481481481481 1.234 0.9375 0. 5461 0.2688 0.1946 0.0554 0.518518518518518 1.18 0.8936 0. 5191 0.265 0.1931 0.05514 0.555555555555556 1.023 0.7693 0. 4035 0.2039 0.1521 0.04719 0.592592592592593 0.8829 0.6763 0. 3348 0.1694 0.1461 0.04646 0.62962962962963 0.6798 0.5623 0. 3214 0.1652 0.1319 0.044 0.666666666666667 0.6638 0.512 0. 2623 0.153 0.127 0.04306 0.703703703703704 0.6564 0.4974 0. 2495 0.1423 0.1159 0.04295 0.740740740740741 0.6363 0.483 0. 2203 0.1334 0.1105 0.03941 0.777777777777778 0.6053 0.461 0. 2115 0.1228 0.1068 0.03677

## 67 0.814814814814815 0.5025 0.3831 0. 211 0.1175 0.09979 0.03532 0.851851851851852 0.4489 0.3427 0. 1935 0.1135 0.08628 0.03323 0.888888888888889 0.2889 0.2248 0. 09501 0.05922 0.06081 0.02247 0.925925925925926 0.2854 0.2171 0. 09265 0.05799 0.05332 0.01879 0.962962962962963 0.1884 0.1433 0. 06158 0.04207 0.02871 0.01104

0.1 3.0315 2.3943 1.0909 0.56855 0.41592 0.11789 Average of yearly averages: 0.0638888461538462

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: ANCAppl2 Metfile: w03812.dvf PRZM scenario: NCappleC.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.9e-5 atm-m^3/mol Vapor Pressure vapr 1.268e-6 torr Solubility sol 0.067 mg/L Kd Kd 27 mg/L Koc Koc mg/L Photolysis half-life kdp 0.19 days Half-life Aerobic Aquatic Metabolism kbacw 97 days Halfife Anaerobic Aquatic Metabolism kbacs 649.8 days Halfife Aerobic Soil Metabolism asm 48.5 days Halfife Hydrolysis: pH 7 9.6 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.34 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 01-07 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 21 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none none, mont hly or total(average of entire run)

## 68 

# NY Grapes – Model Approach 1 stored as ANYgrps1.out Chemical: Acequinocyl PRZM environment: NYGrapesC.txt modifi ed Friday, 8 August 2003 at 09:42:08 EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 17:33:30 Metfile: w14860.dvf modified Wedday, 3 July 2002 at 10:06:14 Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 0.596 0.3474 0.1483 0.1237 0.1139 0.03759 1962 5.365 3.328 1.46 0.9672 0.8656 0.4281 1963 2.079 1.497 0.755 0.7118 0.6778 0.5708 1964 3.563 2.223 1.053 0.7838 0.7229 0.6224 1965 2.27 1.484 0.8568 0.7874 0.7718 0.7044 1966 3.894 2.409 1.186 0.9045 0.8452 0.7517 1967 1.822 1.274 0.8292 0.7544 0.7452 0.6683 1968 2.619 1.786 0.8232 0.7526 0.7101 0.5852 1969 1.887 1.444 0.912 0.7914 0.7798 0.6779 1970 4.619 2.795 1.492 1.168 1.157 0.8578 1971 2.419 1.661 1.071 1.064 1.062 0.9078 1972 1.692 1.277 1.063 0.9878 0.9583 0.8749 1973 3.073 1.985 1.082 0.8711 0.8307 0.7732 1974 2.491 1.662 0.9942 0.8278 0.784 0.7636 1975 5.815 4.265 2.044 1.461 1.322 1.011 1976 1.413 1.262 1.18 1.108 1.047 0.8364 1977 6.089 4.049 2.184 1.61 1.461 1.013 1978 2.759 1.981 1.387 1.277 1.279 1.12 1979 4.966 3.222 1.804 1.561 1.467 1.158 1980 4.25 3.243 1.791 1.533 1.445 1.22 1981 2.744 2.015 1.399 1.337 1.273 1.116 1982 4.189 2.711 1.49 1.186 1.184 1.075 1983 3.255 2.266 1.574 1.355 1.302 1.068 1984 5.224 3.237 1.649 1.25 1.165 1.027 1985 4.232 2.991 1.482 1.215 1.118 0.9394 1986 7.424 4.343 2.014 1.4 1.286 1.02 1987 4.305 3.072 1.739 1.402 1.335 1.06 1988 3.205 2.134 1.298 1.129 1.088 0.9741 1989 2.488 1.66 1.001 0.9261 0.901 0.8281 1990 5.2 3.21 1.736 1.351 1.221 0.8577

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258064516129 7.424 4.343 2.184 1.61 1.467 1.22 0.0645161290322581 6.089 4.265 2.044 1.561 1.461 1.158 0.0967741935483871 5.815 4.049 2.014 1.533 1.445 1.12 0.129032258064516 5.365 3.328 1.804 1.461 1.335 1.116 0.161290322580645 5.224 3.243 1.791 1.402 1.322 1.075 0.193548387096774 5.2 3.237 1.739 1.4 1.302 1.068 0.225806451612903 4.966 3.222 1.736 1.355 1.286 1.06 0.258064516129032 4.619 3.21 1.649 1.351 1.279 1.027 0.290322580645161 4.305 3.072 1.574 1.337 1.273 1.02 0.32258064516129 4.25 2.991 1.492 1.277 1.221 1.013 0.354838709677419 4.232 2.795 1.49 1.25 1.184 1.011 0.387096774193548 4.189 2.711 1.482 1.215 1.165 0.9741 0.419354838709677 3.894 2.409 1.46 1.186 1.157 0.9394 0.451612903225806 3.563 2.266 1.399 1.168 1.118 0.9078 0.483870967741936 3.255 2.223 1.387 1.129 1.088 0.8749 0.516129032258065 3.205 2.134 1.298 1.108 1.062 0.8578 0.548387096774194 3.073 2.015 1.186 1.064 1.047 0.8577

## 69 0.580645161290323 2.759 1.985 1.18 0.9878 0.9583 0.8364 0.612903225806452 2.744 1.981 1.082 0.9672 0.901 0.8281 0.645161290322581 2.619 1.786 1. 071 0.9261 0.8656 0.7732 0.67741935483871 2.491 1.662 1.063 0.9045 0.8452 0.7636 0.709677419354839 2.488 1.661 1. 053 0.8711 0.8307 0.7517 0.741935483870968 2.419 1.66 1.001 0.8278 0.784 0.7044 0.774193548387097 2.27 1.497 0. 9942 0.7914 0.7798 0.6779 0.806451612903226 2.079 1.484 0. 912 0.7874 0.7718 0.6683 0.838709677419355 1.887 1.444 0. 8568 0.7838 0.7452 0.6224 0.870967741935484 1.822 1.277 0. 8292 0.7544 0.7229 0.5852 0.903225806451613 1.692 1.274 0. 8232 0.7526 0.7101 0.5708 0.935483870967742 1.413 1.262 0. 755 0.7118 0.6778 0.4281 0.967741935483871 0.596 0.3474 0. 1483 0.1237 0.1139 0.03759

0.1 5.77 3.9769 1.993 1.5258 1.434 1.1196 Average of yearly averages: 0.851579666666667

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: ANYgrps1 Metfile: w14860.dvf PRZM scenario: NYGrapesC.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.9e-5 atm-m^3/mol Vapor Pressure vapr 1.268e-6 torr Solubility sol 0.067 mg/L Kd Kd 678 mg/L Koc Koc mg/L Photolysis half-life kdp 0 days Half-life Aerobic Aquatic Metabolism kbacw 0 days Halfife Anaerobic Aquatic Metabolism kbacs 0 days Halfife Aerobic Soil Metabolism asm 0 days Halfife Hydrolysis: pH 7 0 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.34 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 15-07 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 21 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none none, mont hly or total(average of entire run)

## 70 

# NY Grapes – Model Approach 2 stored as ANYgrpe2.out Chemical: Acequinocyl PRZM environment: NYGrapesC.txt modifi ed Friday, 8 August 2003 at 09:42:08 EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 17:33:30 Metfile: w14860.dvf modified Wedday, 3 July 2002 at 10:06:14 Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 0.1935 0.1439 0.08515 0.05221 0.04414 0.01339 1962 3.12 2.332 1.113 0.514 0.3893 0.114 1963 0.222 0.1664 0.1124 0.07913 0.057 0.02852 1964 1.62 1.272 0.5443 0.2339 0.1723 0.05107 1965 0.4734 0.353 0.1504 0.08647 0.07234 0.02932 1966 1.72 1.289 0.5525 0.2553 0.1893 0.05942 1967 0.5497 0.4135 0.1831 0.1162 0.1002 0.03399 1968 0.196 0.1444 0.08384 0.0648 0.04828 0.01748 1969 0.1794 0.1322 0.05872 0.04055 0.03016 0.01204 1970 2.818 2.105 0.947 0.506 0.4176 0.1199 1971 1.078 0.8075 0.3834 0.1902 0.1501 0.054 1972 0.262 0.1959 0.1152 0.07197 0.07011 0.02478 1973 0.6296 0.4716 0.2001 0.1068 0.08147 0.02513 1974 0.815 0.6154 0.277 0.1381 0.1072 0.03597 1975 2.451 2.059 1.014 0.5263 0.3966 0.1184 1976 0.3129 0.2339 0.1203 0.08223 0.06932 0.02977 1977 3.726 2.795 1.627 0.8034 0.5928 0.1663 1978 0.9981 0.8319 0.484 0.2776 0.2121 0.07662 1979 2.184 1.637 0.7126 0.6152 0.4757 0.1369 1980 1.209 1.001 0.4763 0.3486 0.2702 0.08681 1981 0.7659 0.6309 0.3209 0.1934 0.16 0.05311 1982 0.5702 0.4325 0.2356 0.1402 0.1111 0.03668 1983 0.8814 0.6711 0.5398 0.3061 0.2717 0.08121 1984 1.205 0.9063 0.4046 0.1771 0.1333 0.04463 1985 0.5001 0.3718 0.1639 0.09094 0.06793 0.02872 1986 1.843 1.351 0.6257 0.246 0.1729 0.05144 1987 1.706 1.31 0.629 0.4908 0.3746 0.1073 1988 0.6202 0.4696 0.2663 0.1737 0.1536 0.05512 1989 0.5974 0.4457 0.1895 0.1293 0.1065 0.03413 1990 1.979 1.487 0.7641 0.451 0.3252 0.09136

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258064516129 3.726 2.795 1. 627 0.8034 0.5928 0.1663 0.0645161290322581 3.12 2.332 1. 113 0.6152 0.4757 0.1369 0.0967741935483871 2.818 2.105 1. 014 0.5263 0.4176 0.1199 0.129032258064516 2.451 2.059 0.947 0.514 0.3966 0.1184 0.161290322580645 2.184 1.637 0.7641 0.506 0.3893 0.114 0.193548387096774 1.979 1.487 0. 7126 0.4908 0.3746 0.1073 0.225806451612903 1.843 1.351 0. 629 0.451 0.3252 0.09136 0.258064516129032 1.72 1.31 0. 6257 0.3486 0.2717 0.08681 0.290322580645161 1.706 1.289 0. 5525 0.3061 0.2702 0.08121 0.32258064516129 1.62 1.272 0. 5443 0.2776 0.2121 0.07662 0.354838709677419 1.209 1.001 0. 5398 0.2553 0.1893 0.05942 0.387096774193548 1.205 0.9063 0. 484 0.246 0.1729 0.05512 0.419354838709677 1.078 0.8319 0. 4763 0.2339 0.1723 0.054 0.451612903225806 0.9981 0.8075 0. 4046 0.1934 0.16 0.05311 0.483870967741936 0.8814 0.6711 0. 3834 0.1902 0.1536 0.05144 0.516129032258065 0.815 0.6309 0. 3209 0.1771 0.1501 0.05107 0.548387096774194 0.7659 0.6154 0. 277 0.1737 0.1333 0.04463

## 71 0.580645161290323 0.6296 0.4716 0. 2663 0.1402 0.1111 0.03668 0.612903225806452 0.6202 0.4696 0. 2356 0.1381 0.1072 0.03597 0.645161290322581 0.5974 0.4457 0. 2001 0.1293 0.1065 0.03413 0.67741935483871 0.5702 0.4325 0. 1895 0.1162 0.1002 0.03399 0.709677419354839 0.5497 0.4135 0. 1831 0.1068 0.08147 0.02977 0.741935483870968 0.5001 0.3718 0. 1639 0.09094 0.07234 0.02932 0.774193548387097 0.4734 0.353 0. 1504 0.08647 0.07011 0.02872 0.806451612903226 0.3129 0.2339 0. 1203 0.08223 0.06932 0.02852 0.838709677419355 0.262 0.1959 0. 1152 0.07913 0.06793 0.02513 0.870967741935484 0.222 0.1664 0. 1124 0.07197 0.057 0.02478 0.903225806451613 0.196 0.1444 0. 08515 0.0648 0.04828 0.01748 0.935483870967742 0.1935 0.1439 0. 08384 0.05221 0.04414 0.01339 0.967741935483871 0.1794 0.1322 0. 05872 0.04055 0.03016 0.01204

0.1 2.7813 2.1004 1.0073 0.52507 0.4155 0.11975 Average of yearly averages: 0.0605836666666667

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: ANYgrpe2 Metfile: w14860.dvf PRZM scenario: NYGrapesC.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.9e-5 atm-m^3/mol Vapor Pressure vapr 1.268e-6 torr Solubility sol 0.067 mg/L Kd Kd 27 mg/L Koc Koc mg/L Photolysis half-life kdp 0.19 days Half-life Aerobic Aquatic Metabolism kbacw 97 days Halfife Anaerobic Aquatic Metabolism kbacs 649.8 days Halfife Aerobic Soil Metabolism asm 48.5 days Halfife Hydrolysis: pH 7 9.6 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.34 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 15-07 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 21 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none none, mont hly or total(average of entire run)

## 72 

# OR Apples – Model Approach 1 stored as AORAppl1.out Chemical: Acequinocyl PRZM environment: ORappl eC.txt modified Satday, 12 October 2002 at 17:16:34 EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 17:33:30 Metfile: w24229.dvf modified Wedday, 3 July 2002 at 10:06:10 Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 0.34 0.2084 0.09484 0.06189 0.04598 0.01604 1962 0.8403 0.5183 0.3214 0.2175 0.1686 0.08797 1963 0.6085 0.3969 0.2277 0.1926 0.1863 0.1718 1964 2.041 1.362 0.5376 0.3127 0.2547 0.1959 1965 1.417 0.9784 0.4245 0.3373 0.3246 0.2873 1966 1.129 0.7574 0.4386 0.3843 0.3417 0.3052 1967 0.6725 0.4708 0.3643 0.334 0.3258 0.2802 1968 0.9544 0.6935 0.4955 0.41 0.3616 0.2945 1969 1.111 0.754 0.5332 0.4446 0.4183 0.3686 1970 1.212 0.8401 0.5849 0.5129 0.4832 0.3957 1971 0.9897 0.7259 0.4659 0.4076 0.3933 0.3574 1972 1.075 0.8182 0.5302 0.4174 0.4046 0.3485 1973 2.614 1.572 0.8115 0.5953 0.4835 0.349 1974 1.639 1.22 0.7116 0.5728 0.5398 0.4413 1975 1.05 0.7587 0.5681 0.4948 0.468 0.3766 1976 0.7851 0.5617 0.3805 0.353 0.3437 0.2851 1977 1.927 1.246 0.7465 0.4655 0.3754 0.243 1978 0.5638 0.4732 0.4025 0.3913 0.3801 0.322 1979 0.8469 0.4641 0.3323 0.3121 0.3003 0.2632 1980 2.838 1.915 0.8579 0.6701 0.5264 0.3709 1981 1.759 1.241 0.7012 0.551 0.5375 0.4722 1982 1.802 1.19 0.7354 0.5927 0.537 0.4832 1983 1.687 1.089 0.6498 0.5637 0.5529 0.4924 1984 2.002 1.254 0.6527 0.531 0.4646 0.4155 1985 0.6883 0.5351 0.4211 0.4093 0.4009 0.3547 1986 1.207 0.7731 0.4083 0.3418 0.3418 0.2953 1987 2.295 1.529 0.8819 0.5041 0.4265 0.3684 1988 0.9614 0.7088 0.4956 0.4493 0.4397 0.3969 1989 0.912 0.6294 0.3954 0.3623 0.3569 0.3139 1990 0.7793 0.6351 0.41 0.3684 0.3456 0.2846

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258064516129 2.838 1.915 0. 8819 0.6701 0.5529 0.4924 0.0645161290322581 2.614 1.572 0. 8579 0.5953 0.5398 0.4832 0.0967741935483871 2.295 1.529 0. 8115 0.5927 0.5375 0.4722 0.129032258064516 2.041 1.362 0. 7465 0.5728 0.537 0.4413 0.161290322580645 2.002 1.254 0. 7354 0.5637 0.5264 0.4155 0.193548387096774 1.927 1.246 0. 7116 0.551 0.4835 0.3969 0.225806451612903 1.802 1.241 0. 7012 0.531 0.4832 0.3957 0.258064516129032 1.759 1.22 0.6527 0.5129 0.468 0.3766 0.290322580645161 1.687 1.19 0. 6498 0.5041 0.4646 0.3709 0.32258064516129 1.639 1.089 0. 5849 0.4948 0.4397 0.3686 0.354838709677419 1.417 0.9784 0. 5681 0.4655 0.4265 0.3684 0.387096774193548 1.212 0.8401 0. 5376 0.4493 0.4183 0.3574 0.419354838709677 1.207 0.8182 0. 5332 0.4446 0.4046 0.3547 0.451612903225806 1.129 0.7731 0. 5302 0.4174 0.4009 0.349 0.483870967741936 1.111 0.7587 0. 4956 0.41 0.3933 0.3485 0.516129032258065 1.075 0.7574 0. 4955 0.4093 0.3801 0.322 0.548387096774194 1.05 0.754 0. 4659 0.4076 0.3754 0.3139

## 73 0.580645161290323 0.9897 0.7259 0. 4386 0.3913 0.3616 0.3052 0.612903225806452 0.9614 0.7088 0. 4245 0.3843 0.3569 0.2953 0.645161290322581 0.9544 0.6935 0. 4211 0.3684 0.3456 0.2945 0.67741935483871 0.912 0.6351 0.41 0.3623 0.3437 0.2873 0.709677419354839 0.8469 0.6294 0. 4083 0.353 0.3418 0.2851 0.741935483870968 0.8403 0.5617 0. 4025 0.3418 0.3417 0.2846 0.774193548387097 0.7851 0.5351 0. 3954 0.3373 0.3258 0.2802 0.806451612903226 0.7793 0.5183 0. 3805 0.334 0.3246 0.2632 0.838709677419355 0.6883 0.4732 0. 3643 0.3127 0.3003 0.243 0.870967741935484 0.6725 0.4708 0. 3323 0.3121 0.2547 0.1959 0.903225806451613 0.6085 0.4641 0. 3214 0.2175 0.1863 0.1718 0.935483870967742 0.5638 0.3969 0. 2277 0.1926 0.1686 0.08797 0.967741935483871 0.34 0.2084 0. 09484 0.06189 0.04598 0.01604

0.1 2.2696 1.5123 0.805 0.59071 0.53745 0.46911 Average of yearly averages: 0.321243666666667

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: AORAppl1 Metfile: w24229.dvf PRZM scenario: ORappleC.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.9e-5 atm-m^3/mol Vapor Pressure vapr 1.268e-6 torr Solubility sol 0.067 mg/L Kd Kd 678 mg/L Koc Koc mg/L Photolysis half-life kdp 0 days Half-life Aerobic Aquatic Metabolism kbacw 0 days Halfife Anaerobic Aquatic Metabolism kbacs 0 days Halfife Aerobic Soil Metabolism asm 0 days Halfife Hydrolysis: pH 7 0 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.34 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 01-07 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 21 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none none, mont hly or total(average of entire run)

## 74 

# OR Apples – Model Approach 2 stored as AORAppl2.out Chemical: Acequinocyl PRZM environment: ORappl eC.txt modified Satday, 12 October 2002 at 17:16:34 EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 17:33:30 Metfile: w24229.dvf modified Wedday, 3 July 2002 at 10:06:10 Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 0.2157 0.1751 0.08418 0.05493 0.04008 0.01606 1962 0.2949 0.2234 0.1546 0.08253 0.06306 0.02341 1963 0.2411 0.1811 0.08797 0.04915 0.03396 0.01689 1964 0.3525 0.2978 0.1225 0.07339 0.04923 0.02154 1965 0.2702 0.2266 0.1211 0.07111 0.04773 0.02246 1966 0.344 0.2794 0.1506 0.08277 0.05778 0.0278 1967 0.4569 0.3476 0.1471 0.07233 0.05429 0.02133 1968 0.5395 0.4117 0.2248 0.1052 0.08639 0.03701 1969 0.7469 0.5747 0.2476 0.1559 0.1271 0.04452 1970 0.277 0.2111 0.1177 0.07462 0.05507 0.02643 1971 0.4802 0.361 0.1482 0.07196 0.06253 0.02824 1972 0.1768 0.1459 0.08121 0.04269 0.03082 0.01797 1973 0.5699 0.4332 0.2415 0.1076 0.07888 0.02934 1974 0.4264 0.3407 0.228 0.1235 0.0859 0.02982 1975 0.1777 0.1329 0.055 0.03796 0.02583 0.0179 1976 0.1773 0.1325 0.06542 0.05525 0.0388 0.01138 1977 1.073 0.7978 0.3241 0.138 0.1068 0.03816 1978 0.1777 0.133 0.05386 0.03801 0.02826 0.01171 1979 0.1767 0.1309 0.06155 0.04257 0.0328 0.01678 1980 0.6755 0.5261 0.2503 0.1889 0.1262 0.04117 1981 0.594 0.4518 0.1896 0.1181 0.1145 0.03996 1982 0.6495 0.491 0.2057 0.1095 0.08783 0.03359 1983 0.348 0.2614 0.1271 0.05726 0.04875 0.02224 1984 0.6282 0.4723 0.2129 0.1373 0.09482 0.03065 1985 0.177 0.1313 0.05772 0.03705 0.03809 0.01536 1986 0.8137 0.6208 0.2707 0.1108 0.09118 0.03093 1987 0.8743 0.7094 0.4116 0.1551 0.1037 0.03549 1988 0.2299 0.1744 0.109 0.05526 0.04442 0.02275 1989 0.1778 0.1335 0.05444 0.03838 0.02615 0.01468 1990 0.1773 0.1323 0.05968 0.04639 0.03215 0.01752

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258064516129 1.073 0.7978 0. 4116 0.1889 0.1271 0.04452 0.0645161290322581 0.8743 0.7094 0. 3241 0.1559 0.1262 0.04117 0.0967741935483871 0.8137 0.6208 0. 2707 0.1551 0.1145 0.03996 0.129032258064516 0.7469 0.5747 0. 2503 0.138 0.1068 0.03816 0.161290322580645 0.6755 0.5261 0. 2476 0.1373 0.1037 0.03701 0.193548387096774 0.6495 0.491 0. 2415 0.1235 0.09482 0.03549 0.225806451612903 0.6282 0.4723 0. 228 0.1181 0.09118 0.03359 0.258064516129032 0.594 0.4518 0. 2248 0.1108 0.08783 0.03093 0.290322580645161 0.5699 0.4332 0. 2129 0.1095 0.08639 0.03065 0.32258064516129 0.5395 0.4117 0. 2057 0.1076 0.0859 0.02982 0.354838709677419 0.4802 0.361 0. 1896 0.1052 0.07888 0.02934 0.387096774193548 0.4569 0.3476 0. 1546 0.08277 0.06306 0.02824 0.419354838709677 0.4264 0.3407 0. 1506 0.08253 0.06253 0.0278 0.451612903225806 0.3525 0.2978 0. 1482 0.07462 0.05778 0.02643 0.483870967741936 0.348 0.2794 0. 1471 0.07339 0.05507 0.02341 0.516129032258065 0.344 0.2614 0. 1271 0.07233 0.05429 0.02275 0.548387096774194 0.2949 0.2266 0. 1225 0.07196 0.04923 0.02246

## 75 0.580645161290323 0.277 0.2234 0. 1211 0.07111 0.04875 0.02224 0.612903225806452 0.2702 0.2111 0. 1177 0.05726 0.04773 0.02154 0.645161290322581 0.2411 0.1811 0. 109 0.05526 0.04442 0.02133 0.67741935483871 0.2299 0.1751 0. 08797 0.05525 0.04008 0.01797 0.709677419354839 0.2157 0.1744 0. 08418 0.05493 0.0388 0.0179 0.741935483870968 0.1778 0.1459 0. 08121 0.04915 0.03809 0.01752 0.774193548387097 0.1777 0.1335 0. 06542 0.04639 0.03396 0.01689 0.806451612903226 0.1777 0.133 0. 06155 0.04269 0.0328 0.01678 0.838709677419355 0.1773 0.1329 0. 05968 0.04257 0.03215 0.01606 0.870967741935484 0.1773 0.1325 0. 05772 0.03838 0.03082 0.01536 0.903225806451613 0.177 0.1323 0. 055 0.03801 0.02826 0.01468 0.935483870967742 0.1768 0.1313 0. 05444 0.03796 0.02615 0.01171 0.967741935483871 0.1767 0.1309 0. 05386 0.03705 0.02583 0.01138

0.1 0.80702 0.61619 0.26866 0.15339 0.11373 0.03978 Average of yearly averages: 0.0254363333333333

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: AORAppl2 Metfile: w24229.dvf PRZM scenario: ORappleC.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.9e-5 atm-m^3/mol Vapor Pressure vapr 1.268e-6 torr Solubility sol 0.067 mg/L Kd Kd 27 mg/L Koc Koc mg/L Photolysis half-life kdp 0.19 days Half-life Aerobic Aquatic Metabolism kbacw 97 days Halfife Anaerobic Aquatic Metabolism kbacs 649.8 days Halfife Aerobic Soil Metabolism asm 48.5 days Halfife Hydrolysis: pH 7 9.6 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.34 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 01-07 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 21 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none none, mont hly or total(average of entire run)

## 76 

# OR Christmas Trees – Model Approach 1

stored as AORxmas1.out Chemical: Acequinocyl PRZM environment: ORXmasTr eeC.txt modified Satday, 12 October 2002 at 17:23:10 EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 17:33:30 Metfile: w24232.dvf modified Wedday, 3 July 2002 at 10:06:10 Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 0.07868 0.0478 0.02576 0.01993 0.01738 0.007633 1962 0.2535 0.1487 0.06339 0.04109 0.03603 0.02359 1963 0.1935 0.1413 0.06802 0.06019 0.05694 0.05029 1964 0.6752 0.4637 0.168 0.09514 0.07946 0.06038 1965 0.3724 0.255 0.1326 0.1062 0.1004 0.0912 1966 0.3878 0.2541 0.1344 0.109 0.1095 0.09813 1967 0.4371 0.2768 0.144 0.1126 0.1052 0.09267 1968 0.423 0.2724 0.1772 0.1293 0.1249 0.1033 1969 0.4124 0.2878 0.1687 0.1364 0.1226 0.1107 1970 0.6195 0.3328 0.1822 0.1571 0.1471 0.1247 1971 0.3592 0.2471 0.1599 0.134 0.1295 0.1212 1972 0.8926 0.5324 0.236 0.1706 0.1561 0.1277 1973 0.7582 0.4558 0.2436 0.1932 0.1584 0.118 1974 0.8648 0.5797 0.2839 0.2096 0.1968 0.1599 1975 0.3034 0.2224 0.1539 0.1423 0.1388 0.1234 1976 0.3611 0.2636 0.1533 0.127 0.1229 0.1079 1977 0.3819 0.2914 0.17 0.1261 0.1087 0.08643 1978 0.312 0.212 0.1285 0.1136 0.1094 0.1025 1979 0.3047 0.2048 0.1255 0.1074 0.1027 0.09672 1980 0.9108 0.6347 0.264 0.1947 0.1588 0.1167 1981 0.5462 0.361 0.2379 0.2001 0.1881 0.1566 1982 0.4309 0.2948 0.2044 0.1814 0.1745 0.1553 1983 0.4497 0.3194 0.2105 0.1856 0.175 0.1544 1984 0.546 0.3473 0.1954 0.174 0.154 0.1345 1985 0.2703 0.1929 0.1416 0.138 0.1339 0.1202 1986 0.4284 0.2834 0.1519 0.1185 0.1122 0.09828 1987 0.82 0.5378 0.2868 0.161 0.1355 0.1056 1988 0.4358 0.2931 0.1916 0.1551 0.1459 0.1238 1989 1.115 0.6632 0.2677 0.1573 0.1374 0.1104 1990 0.3648 0.2604 0.1761 0.1603 0.1511 0.1283

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258064516129 1.115 0.6632 0. 2868 0.2096 0.1968 0.1599 0.0645161290322581 0.9108 0.6347 0. 2839 0.2001 0.1881 0.1566 0.0967741935483871 0.8926 0.5797 0. 2677 0.1947 0.175 0.1553 0.129032258064516 0.8648 0.5378 0. 264 0.1932 0.1745 0.1544 0.161290322580645 0.82 0.5324 0. 2436 0.1856 0.1588 0.1345 0.193548387096774 0.7582 0.4637 0. 2379 0.1814 0.1584 0.1283 0.225806451612903 0.6752 0.4558 0. 236 0.174 0.1561 0.1277 0.258064516129032 0.6195 0.361 0. 2105 0.1706 0.154 0.1247 0.290322580645161 0.5462 0.3473 0. 2044 0.161 0.1511 0.1238 0.32258064516129 0.546 0.3328 0. 1954 0.1603 0.1471 0.1234 0.354838709677419 0.4497 0.3194 0. 1916 0.1573 0.1459 0.1212 0.387096774193548 0.4371 0.2948 0. 1822 0.1571 0.1388 0.1202 0.419354838709677 0.4358 0.2931 0. 1772 0.1551 0.1374 0.118 0.451612903225806 0.4309 0.2914 0. 1761 0.1423 0.1355 0.1167 0.483870967741936 0.4284 0.2878 0. 17 0.138 0.1339 0.1107 0.516129032258065 0.423 0.2834 0. 1687 0.1364 0.1295 0.1104 0.548387096774194 0.4124 0.2768 0. 168 0.134 0.1249 0.1079

## 77 0.580645161290323 0.3878 0.2724 0. 1599 0.1293 0.1229 0.1056 0.612903225806452 0.3819 0.2636 0. 1539 0.127 0.1226 0.1033 0.645161290322581 0.3724 0.2604 0. 1533 0.1261 0.1122 0.1025 0.67741935483871 0.3648 0.255 0. 1519 0.1185 0.1095 0.09828 0.709677419354839 0.3611 0.2541 0. 144 0.1136 0.1094 0.09813 0.741935483870968 0.3592 0.2471 0. 1416 0.1126 0.1087 0.09672 0.774193548387097 0.312 0.2224 0. 1344 0.109 0.1052 0.09267 0.806451612903226 0.3047 0.212 0. 1326 0.1074 0.1027 0.0912 0.838709677419355 0.3034 0.2048 0. 1285 0.1062 0.1004 0.08643 0.870967741935484 0.2703 0.1929 0. 1255 0.09514 0.07946 0.06038 0.903225806451613 0.2535 0.1487 0. 06802 0.06019 0.05694 0.05029 0.935483870967742 0.1935 0.1413 0. 06339 0.04109 0.03603 0.02359 0.967741935483871 0.07868 0.0478 0. 02576 0.01993 0.01738 0.007633

0.1 0.88982 0.57551 0.26733 0.19455 0.17495 0.15521 Average of yearly averages: 0.1070141

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: AORxmas1 Metfile: w24232.dvf PRZM scenario: ORXmasTreeC.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.9e-5 atm-m^3/mol Vapor Pressure vapr 1.268e-6 torr Solubility sol 0.067 mg/L Kd Kd 678 mg/L Koc Koc mg/L Photolysis half-life kdp 0 days Half-life Aerobic Aquatic Metabolism kbacw 0 days Halfife Anaerobic Aquatic Metabolism kbacs 0 days Halfife Aerobic Soil Metabolism asm 0 days Halfife Hydrolysis: pH 7 0 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.134 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 01-07 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 14 days Set to 0 or delete line for single app. Interval 2 interval 14 days Set to 0 or delete line for single app. Interval 3 interval 14 days Set to 0 or delete line for single app. Interval 4 interval 14 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none none, monthl y or total (average of entire run)

## 78 

# OR Christmas Trees – Model Approach 2

stored as AORXmas2.out Chemical: Acequinocyl PRZM environment: ORXmasTr eeC.txt modified Satday, 12 October 2002 at 17:23:10 EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 17:33:30 Metfile: w24232.dvf modified Wedday, 3 July 2002 at 10:06:10 Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 0.07827 0.05975 0.03923 0.03389 0.02646 0.008472 1962 0.1432 0.1078 0.04642 0.03344 0.026 0.01254 1963 0.1798 0.1373 0.06181 0.03408 0.02694 0.01259 1964 0.1831 0.1466 0.05859 0.03311 0.02578 0.01285 1965 0.12 0.09195 0.04527 0.03307 0.02573 0.01283 1966 0.1081 0.08482 0.03965 0.03257 0.02521 0.01306 1967 0.1198 0.09025 0.0378 0.03271 0.02526 0.01158 1968 0.2064 0.1675 0.08108 0.05615 0.04905 0.01873 1969 0.1536 0.1194 0.05286 0.03604 0.03427 0.01559 1970 0.08419 0.06451 0.03768 0.0326 0.02542 0.01053 1971 0.3476 0.2611 0.1139 0.06075 0.04931 0.01618 1972 0.07765 0.06217 0.03832 0.03278 0.02762 0.01116 1973 0.2231 0.1691 0.09549 0.04424 0.03012 0.01504 1974 0.1117 0.09257 0.05978 0.0339 0.02637 0.01232 1975 0.07776 0.05927 0.03861 0.03403 0.02632 0.01012 1976 0.07912 0.0609 0.04034 0.03445 0.02691 0.007477 1977 0.0886 0.07139 0.04246 0.03363 0.02699 0.01144 1978 0.07823 0.05963 0.03917 0.03429 0.02764 0.009695 1979 0.3346 0.2544 0.1128 0.05943 0.04894 0.01574 1980 0.2354 0.1813 0.08542 0.04998 0.03374 0.01553 1981 0.2554 0.1986 0.1194 0.06521 0.05449 0.02206 1982 0.07821 0.05963 0.03909 0.03356 0.02609 0.01097 1983 0.158 0.1201 0.05785 0.04284 0.03463 0.01238 1984 0.2506 0.1916 0.09377 0.04922 0.035 0.01554 1985 0.09143 0.06971 0.03788 0.03234 0.02516 0.009709 1986 0.07691 0.05781 0.03732 0.03267 0.02529 0.008699 1987 0.357 0.2773 0.1607 0.06195 0.04188 0.02004 1988 0.1465 0.1122 0.0519 0.03186 0.02467 0.0124 1989 0.241 0.1887 0.08449 0.0411 0.02876 0.01417 1990 0.09762 0.07449 0.03876 0.03279 0.02545 0.01179

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258064516129 0.357 0.2773 0. 1607 0.06521 0.05449 0.02206 0.0645161290322581 0.3476 0.2611 0. 1194 0.06195 0.04931 0.02004 0.0967741935483871 0.3346 0.2544 0. 1139 0.06075 0.04905 0.01873 0.129032258064516 0.2554 0.1986 0. 1128 0.05943 0.04894 0.01618 0.161290322580645 0.2506 0.1916 0. 09549 0.05615 0.04188 0.01574 0.193548387096774 0.241 0.1887 0. 09377 0.04998 0.035 0.01559 0.225806451612903 0.2354 0.1813 0. 08542 0.04922 0.03463 0.01554 0.258064516129032 0.2231 0.1691 0. 08449 0.04424 0.03427 0.01553 0.290322580645161 0.2064 0.1675 0. 08108 0.04284 0.03374 0.01504 0.32258064516129 0.1831 0.1466 0. 06181 0.0411 0.03012 0.01417 0.354838709677419 0.1798 0.1373 0. 05978 0.03604 0.02876 0.01306 0.387096774193548 0.158 0.1201 0. 05859 0.03445 0.02764 0.01285 0.419354838709677 0.1536 0.1194 0. 05785 0.03429 0.02762 0.01283 0.451612903225806 0.1465 0.1122 0. 05286 0.03408 0.02699 0.01259 0.483870967741936 0.1432 0.1078 0. 0519 0.03403 0.02694 0.01254 0.516129032258065 0.12 0.09257 0. 04642 0.0339 0.02691 0.0124 0.548387096774194 0.1198 0.09195 0. 04527 0.03389 0.02646 0.01238

## 79 0.580645161290323 0.1117 0.09025 0. 04246 0.03363 0.02637 0.01232 0.612903225806452 0.1081 0.08482 0. 04034 0.03356 0.02632 0.01179 0.645161290322581 0.09762 0.07449 0. 03965 0.03344 0.02609 0.01158 0.67741935483871 0.09143 0.07139 0. 03923 0.03311 0.026 0.01144 0.709677419354839 0.0886 0.06971 0. 03917 0.03307 0.02578 0.01116 0.741935483870968 0.08419 0.06451 0. 03909 0.03279 0.02573 0.01097 0.774193548387097 0.07912 0.06217 0. 03876 0.03278 0.02545 0.01053 0.806451612903226 0.07827 0.0609 0. 03861 0.03271 0.02542 0.01012 0.838709677419355 0.07823 0.05975 0. 03832 0.03267 0.02529 0.009709 0.870967741935484 0.07821 0.05963 0. 03788 0.0326 0.02526 0.009695 0.903225806451613 0.07776 0.05963 0. 0378 0.03257 0.02521 0.008699 0.935483870967742 0.07765 0.05927 0. 03768 0.03234 0.02516 0.008472 0.967741935483871 0.07691 0.05781 0. 03732 0.03186 0.02467 0.007477

0.1 0.32668 0.24882 0.11379 0.060618 0.049039 0.018475 Average of yearly averages: 0.0130410666666667

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: AORXmas2 Metfile: w24232.dvf PRZM scenario: ORXmasTreeC.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.9e-5 atm-m^3/mol Vapor Pressure vapr 1.268e-6 torr Solubility sol 0.067 mg/L Kd Kd 27 mg/L Koc Koc mg/L Photolysis half-life kdp 0.19 days Half-life Aerobic Aquatic Metabolism kbacw 97 days Halfife Anaerobic Aquatic Metabolism kbacs 649.8 days Halfife Aerobic Soil Metabolism asm 48.5 days Halfife Hydrolysis: pH 7 9.6 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.134 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 01-07 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 14 days Set to 0 or delete line for single app. Interval 2 interval 14 days Set to 0 or delete line for single app. Interval 3 interval 14 days Set to 0 or delete line for single app. Interval 4 interval 14 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none none, mont hly or total(average of entire run)

## 80 

# PA Apples – Model Approach 1 stored as APAappl1.out Chemical: Acequinocyl PRZM environment: PAappl eC.txt modified Satday, 12 October 2002 at 17:24:46 EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 17:33:30 Metfile: w14737.dvf modified Wedday, 3 July 2002 at 10:06:12 Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 2.29 1.354 0.7568 0.4549 0.3948 0.1675 1962 3.792 2.357 1.052 0.7766 0.7272 0.501 1963 4.574 2.836 1.406 1.09 1.032 0.739 1964 1.871 1.318 1.034 1.01 0.9684 0.8097 1965 2.4 1.651 1.209 1.034 0.978 0.8392 1966 3.505 2.242 1.469 1.248 1.188 0.9589 1967 2.351 1.72 1.239 1.204 1.171 1.073 1968 1.787 1.437 1.127 1.094 1.077 0.9728 1969 7.528 5.67 2.746 1.879 1.697 1.148 1970 2.724 2.009 1.46 1.396 1.343 1.237 1971 5.646 4.439 2.248 1.85 1.72 1.349 1972 4.459 2.952 1.918 1.526 1.401 1.295 1973 5.593 3.56 1.843 1.467 1.395 1.268 1974 3.918 2.75 2.097 1.732 1.625 1.31 1975 3.497 2.574 1.651 1.439 1.372 1.227 1976 6.458 4.108 1.934 1.502 1.42 1.175 1977 2.581 1.882 1.408 1.327 1.295 1.18 1978 5.31 3.464 2.382 1.795 1.663 1.334 1979 4.498 3.017 1.966 1.608 1.513 1.351 1980 2.358 1.675 1.389 1.382 1.329 1.091 1981 2.172 1.563 1.12 1.05 1.002 0.9087 1982 8.087 5.688 2.566 1.839 1.685 1.209 1983 2.951 2.373 1.653 1.326 1.246 1.144 1984 3.634 2.482 1.616 1.433 1.354 1.226 1985 6.27 3.882 2.067 1.64 1.645 1.264 1986 3.341 2.339 1.569 1.546 1.48 1.271 1987 6.586 4.143 2.038 1.841 1.7 1.284 1988 3.605 2.483 1.665 1.499 1.43 1.312 1989 3.616 2.569 1.553 1.349 1.294 1.197 1990 5.728 3.636 1.908 1.487 1.403 1.169

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258064516129 8.087 5.688 2.746 1.879 1.72 1.351 0.0645161290322581 7.528 5.67 2.566 1.85 1.7 1.349 0.0967741935483871 6.586 4.439 2.382 1.841 1.697 1.334 0.129032258064516 6.458 4.143 2.248 1.839 1.685 1.312 0.161290322580645 6.27 4.108 2.097 1.795 1.663 1.31 0.193548387096774 5.728 3.882 2.067 1.732 1.645 1.295 0.225806451612903 5.646 3.636 2.038 1.64 1.625 1.284 0.258064516129032 5.593 3.56 1.966 1.608 1.513 1.271 0.290322580645161 5.31 3.464 1.934 1.546 1.48 1.268 0.32258064516129 4.574 3.017 1.918 1.526 1.43 1.264 0.354838709677419 4.498 2.952 1.908 1.502 1.42 1.237 0.387096774193548 4.459 2.836 1.843 1.499 1.403 1.227 0.419354838709677 3.918 2.75 1.665 1.487 1.401 1.226 0.451612903225806 3.792 2.574 1.653 1.467 1.395 1.209 0.483870967741936 3.634 2.569 1.651 1.439 1.372 1.197 0.516129032258065 3.616 2.483 1.616 1.433 1.354 1.18 0.548387096774194 3.605 2.482 1.569 1.396 1.343 1.175

## 81 0.580645161290323 3.505 2.373 1.553 1.382 1.329 1.169 0.612903225806452 3.497 2.357 1.469 1.349 1.295 1.148 0.645161290322581 3.341 2.339 1.46 1.327 1.294 1.144 0.67741935483871 2.951 2.242 1.408 1.326 1.246 1.091 0.709677419354839 2.724 2.009 1.406 1.248 1.188 1.073 0.741935483870968 2.581 1.882 1.389 1.204 1.171 0.9728 0.774193548387097 2.4 1.72 1.239 1.094 1.077 0.9589 0.806451612903226 2.358 1.675 1.209 1.09 1.032 0.9087 0.838709677419355 2.351 1.651 1.127 1.05 1.002 0.8392 0.870967741935484 2.29 1.563 1.12 1.034 0.978 0.8097 0.903225806451613 2.172 1.437 1.052 1.01 0.9684 0.739 0.935483870967742 1.871 1.354 1.034 0.7766 0.7272 0.501 0.967741935483871 1.787 1.318 0. 7568 0.4549 0.3948 0.1675

0.1 6.5732 4.4094 2.3686 1.8408 1.6958 1.3318 Average of yearly averages: 1.10036

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: APAappl1 Metfile: w14737.dvf PRZM scenario: PAappleC.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.9e-5 atm-m^3/mol Vapor Pressure vapr 1.268e-6 torr Solubility sol 0.067 mg/L Kd Kd 678 mg/L Koc Koc mg/L Photolysis half-life kdp 0 days Half-life Aerobic Aquatic Metabolism kbacw 0 days Halfife Anaerobic Aquatic Metabolism kbacs 0 days Halfife Aerobic Soil Metabolism asm 0 days Halfife Hydrolysis: pH 7 0 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.34 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 01-07 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 21 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none none, mont hly or total(average of entire run)

## 82 

# PA Apples – Model Approach 2 stored as APAAppl2.out Chemical: Acequinocyl PRZM environment: PAappl eC.txt modified Satday, 12 October 2002 at 17:24:46 EXAMS environment: pond298.exv modified Thuday, 29 August 2002 at 17:33:30 Metfile: w14737.dvf modified Wedday, 3 July 2002 at 10:06:12 Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 2.566 1.982 1.26 0.5895 0.4257 0.1216 1962 1.98 1.575 0.736 0.3491 0.2587 0.08894 1963 1.168 0.8989 0.4442 0.2607 0.2147 0.07621 1964 0.2368 0.181 0.1212 0.06752 0.04866 0.02625 1965 0.6019 0.4635 0.3236 0.1807 0.14 0.04646 1966 1.142 0.8638 0.3926 0.2776 0.2081 0.06887 1967 1.193 0.9165 0.4262 0.2831 0.2156 0.07001 1968 0.1823 0.137 0.07547 0.0519 0.04578 0.02075 1969 5.343 4.152 2.106 0.9221 0.6578 0.1829 1970 0.912 0.7028 0.4791 0.2485 0.1935 0.07923 1971 3.19 2.544 1.255 0.7026 0.5154 0.1527 1972 0.9843 0.806 0.441 0.2314 0.1747 0.07826 1973 1.091 0.8275 0.3574 0.1643 0.1383 0.05282 1974 1.557 1.225 0.9631 0.5204 0.383 0.1129 1975 0.7191 0.5845 0.4303 0.2179 0.2045 0.07069 1976 3.396 2.675 1.169 0.5531 0.4127 0.1224 1977 0.4441 0.3605 0.2566 0.157 0.1245 0.05222 1978 2.35 1.889 1.144 0.5633 0.4295 0.1255 1979 1.03 0.8025 0.5369 0.3387 0.3137 0.1007 1980 0.2098 0.1577 0.06575 0.04423 0.031 0.02032 1981 0.207 0.1538 0.0637 0.04691 0.04215 0.01493 1982 4.922 4.02 1.803 0.7979 0.5729 0.1619 1983 0.7468 0.5691 0.2533 0.1403 0.1103 0.05165 1984 1.774 1.35 0.6737 0.421 0.3065 0.09285 1985 3.612 2.739 1.369 0.6143 0.5579 0.1644 1986 1.166 0.8925 0.4275 0.2236 0.1707 0.06198 1987 3.099 2.388 1.053 0.6633 0.5153 0.1494 1988 1.83 1.39 0.767 0.4236 0.3169 0.1046 1989 0.7509 0.5726 0.2719 0.1537 0.1639 0.05817 1990 3.216 2.453 1.115 0.6247 0.4642 0.14

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258064516129 5.343 4.152 2. 106 0.9221 0.6578 0.1829 0.0645161290322581 4.922 4.02 1. 803 0.7979 0.5729 0.1644 0.0967741935483871 3.612 2.739 1. 369 0.7026 0.5579 0.1619 0.129032258064516 3.396 2.675 1.26 0.6633 0.5154 0.1527 0.161290322580645 3.216 2.544 1. 255 0.6247 0.5153 0.1494 0.193548387096774 3.19 2.453 1.169 0.6143 0.4642 0.14 0.225806451612903 3.099 2.388 1. 144 0.5895 0.4295 0.1255 0.258064516129032 2.566 1.982 1. 115 0.5633 0.4257 0.1224 0.290322580645161 2.35 1.889 1.053 0.5531 0.4127 0.1216 0.32258064516129 1.98 1.575 0.9631 0.5204 0.383 0.1129 0.354838709677419 1.83 1.39 0.767 0.4236 0.3169 0.1046 0.387096774193548 1.774 1.35 0.736 0.421 0.3137 0.1007 0.419354838709677 1.557 1.225 0. 6737 0.3491 0.3065 0.09285 0.451612903225806 1.193 0.9165 0. 5369 0.3387 0.2587 0.08894 0.483870967741936 1.168 0.8989 0. 4791 0.2831 0.2156 0.07923 0.516129032258065 1.166 0.8925 0. 4442 0.2776 0.2147 0.07826 0.548387096774194 1.142 0.8638 0. 441 0.2607 0.2081 0.07621

## 83 0.580645161290323 1.091 0.8275 0. 4303 0.2485 0.2045 0.07069 0.612903225806452 1.03 0.806 0. 4275 0.2314 0.1935 0.07001 0.645161290322581 0.9843 0.8025 0. 4262 0.2236 0.1747 0.06887 0.67741935483871 0.912 0.7028 0. 3926 0.2179 0.1707 0.06198 0.709677419354839 0.7509 0.5845 0. 3574 0.1807 0.1639 0.05817 0.741935483870968 0.7468 0.5726 0. 3236 0.1643 0.14 0.05282 0.774193548387097 0.7191 0.5691 0. 2719 0.157 0.1383 0.05222 0.806451612903226 0.6019 0.4635 0. 2566 0.1537 0.1245 0.05165 0.838709677419355 0.4441 0.3605 0. 2533 0.1403 0.1103 0.04646 0.870967741935484 0.2368 0.181 0. 1212 0.06752 0.04866 0.02625 0.903225806451613 0.2098 0.1577 0. 07547 0.0519 0.04578 0.02075 0.935483870967742 0.207 0.1538 0. 06575 0.04691 0.04215 0.02032 0.967741935483871 0.1823 0.137 0. 0637 0.04423 0.031 0.01493

0.1 3.5904 2.7326 1.3581 0.69867 0.55365 0.16098 Average of yearly averages: 0.088987

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: APAAppl2 Metfile: w14737.dvf PRZM scenario: PAappleC.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.9e-5 atm-m^3/mol Vapor Pressure vapr 1.268e-6 torr Solubility sol 0.067 mg/L Kd Kd 27 mg/L Koc Koc mg/L Photolysis half-life kdp 0.19 days Half-life Aerobic Aquatic Metabolism kbacw 97 days Halfife Anaerobic Aquatic Metabolism kbacs 649.8 days Halfife Aerobic Soil Metabolism asm 48.5 days Halfife Hydrolysis: pH 7 9.6 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.34 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 01-07 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 21 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none none, mont hly or total(average of entire run)

## 84 

# FL Nursery Plants – Model Approach 1

stored as AFLnurs1.out Chemical: Acequinocyl PRZM environment: FLnursery no_irrig.txt "modi fied Tueday, 17 April 2007 at 08:28:20"

EXAMS environment: pond298.exv "modified Thuday , 29 August 2002 at 17:33:30" Metfile: w12839.dvf "modified Wedday , 3 July 2002 at 09:04:28" Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 0.8075 0.5126 0.2688 0.2013 0.188 0.101 1962 1.297 0.8383 0.4836 0.388 0.3599 0.234 1963 1.954 1.223 0.65 0.4645 0.4221 0.3201 1964 3.738 2.216 0.961 0.7373 0.7066 0.4881 1965 3.277 2.052 1.181 0.9597 0.8819 0.6163 1966 3.526 2.4 1.183 1.06 0.9924 0.7938 1967 3.809 2.659 1.337 1.019 0.9382 0.773 1968 2.772 1.907 1.416 1.341 1.242 0.9313 1969 2.137 1.512 1.125 0.9187 0.9028 0.8284 1970 3.158 2.006 1.211 0.9031 0.8309 0.7027 1971 2.075 1.51 0.9998 0.7784 0.7594 0.6166 1972 3.147 2.209 1.132 0.9459 0.9013 0.6859 1973 2.166 1.467 1.063 0.8162 0.751 0.6123 1974 2.839 1.839 0.8766 0.7058 0.6965 0.5707 1975 1.162 0.9182 0.6607 0.5518 0.5189 0.4706 1976 2.599 1.713 0.9072 0.7485 0.7099 0.5458 1977 8.835 4.903 1.817 1.471 1.306 0.8607 1978 1.282 1.03 0.7903 0.7456 0.7337 0.6524 1979 11.44 6.178 2.084 1.286 1.14 0.7836 1980 2.8 1.829 1.245 1.049 1.009 0.8266 1981 3.646 2.514 1.3 1.192 1.077 0.7736 1982 4.869 2.994 1.373 1.145 1.09 0.8653 1983 2.672 1.858 1.104 0.9698 0.9418 0.8212 1984 4.333 2.923 1.475 1.187 1.131 0.883 1985 2.5 1.734 1.379 1.141 1.105 0.8046 1986 2.714 1.977 1.164 1.017 0.9721 0.8074 1987 2.831 1.733 1.046 0.8266 0.7875 0.6485 1988 2.26 1.538 0.9909 0.8344 0.8156 0.619 1989 2.424 1.581 1.001 0.7996 0.738 0.5525 1990 2.715 1.643 1.082 0.8685 0.839 0.6649

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258065 11.44 6.178 2.084 1.471 1.306 0.9313 0.064516129 8.835 4.903 1.817 1.341 1.242 0.883 0.096774194 4.869 2.994 1.475 1.286 1.14 0.8653 0.129032258 4.333 2.923 1.416 1.192 1.131 0.8607 0.161290323 3.809 2.659 1.379 1.187 1.105 0.8284 0.193548387 3.738 2.514 1.373 1.145 1.09 0.8266 0.225806452 3.646 2.4 1.337 1.141 1.077 0.8212 0.258064516 3.526 2.216 1.3 1.06 1.009 0.8074 0.290322581 3.277 2.209 1.245 1.049 0.9924 0.8046 0.322580645 3.158 2.052 1.211 1.019 0.9721 0.7938 0.35483871 3.147 2.006 1.183 1.017 0.9418 0.7836 0.387096774 2.839 1.977 1.181 0.9698 0.9382 0.7736 0.419354839 2.831 1.907 1.164 0.9597 0.9028 0.773 0.451612903 2.8 1.858 1.132 0.9459 0.9013 0.7027 0.483870968 2.772 1.839 1.125 0.9187 0.8819 0.6859 0.516129032 2.715 1.829 1.104 0.9031 0.839 0.6649

## 85 0.548387097 2.714 1.734 1.082 0.8685 0.8309 0.6524 0.580645161 2.672 1.733 1.063 0.8344 0.8156 0.6485 0.612903226 2.599 1.713 1.046 0.8266 0.7875 0.619 0.64516129 2.5 1.643 1.001 0.8162 0.7594 0.6166 0.677419355 2.424 1.581 0.9998 0.7996 0.751 0.6163 0.709677419 2.26 1.538 0.9909 0.7784 0.738 0.6123 0.741935484 2.166 1.512 0.961 0.7485 0.7337 0.5707 0.774193548 2.137 1.51 0.9072 0.7456 0.7099 0.5525 0.806451613 2.075 1.467 0.8766 0.7373 0.7066 0.5458 0.838709677 1.954 1.223 0.7903 0.7058 0.6965 0.4881 0.870967742 1.297 1.03 0.6607 0.5518 0.5189 0.4706 0.903225806 1.282 0.9182 0.65 0.4645 0.4221 0.3201 0.935483871 1.162 0.8383 0.4836 0.388 0.3599 0.234 0.967741935 0.8075 0.5126 0.2688 0.2013 0.188 0.101

0.1 4.8154 2.9869 1.4691 1.2766 1.1391 0.86484 Average of yearly averages: 0.661796667

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: AFLnurs1 Metfile: w12839.dvf PRZM scenario: FLnursery no_irrig.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.90E-05 atm-m^3/mol Vapor Pressure vapr 1.27E-06 torr Solubility sol 0.067 mg/L Kd Kd 678 mg/L Koc Koc mg/L Photolysis half-life kdp 0 days Half-life Aerobic Aquatic Metabolism kbacw 0 days Halfife Anaerobic Aquatic Metabolism kbacs 0 days Halfife Aerobic Soil Metabolism asm 0 days Halfife Hydrolysis: pH 7 0 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.134 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 15-03 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 14 days Set to 0 or delete line for single app. Interval 2 interval 14 days Set to 0 or delete line for single app. Interval 3 interval 14 days Set to 0 or delete line for single app. Interval 4 interval 14 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none "none, mont hly or total(average of entire run)"

## 86 

# FL Nursery Plants – Model Approach 2

stored as AFLnurs2.out Chemical: Acequinocyl PRZM environment: FLnursery no_irrig.txt "modi fied Tueday, 17 April 2007 at 08:28:20"

EXAMS environment: pond298.exv "modified Thuday , 29 August 2002 at 17:33:30" Metfile: w12839.dvf "modified Wedday , 3 July 2002 at 09:04:28" Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 3.02 2.19 1.258 0.6179 0.4274 0.1188 1962 1.954 1.547 0.9595 0.4026 0.2937 0.08347 1963 2.776 1.997 0.7635 0.3949 0.2755 0.07866 1964 2.246 1.801 0.8664 0.585 0.4124 0.1168 1965 1.315 0.953 0.4744 0.2172 0.1669 0.06249 1966 3.431 2.595 1.163 0.7029 0.4866 0.1444 1967 4.495 3.482 1.598 0.6552 0.4678 0.1373 1968 4.28 3.387 2.412 1.302 0.8938 0.231 1969 1.218 0.9258 0.5401 0.454 0.3397 0.09419 1970 5.581 4.107 2.156 0.8608 0.5881 0.1582 1971 2.209 1.584 0.8017 0.5447 0.3824 0.1052 1972 4.026 3.384 1.546 0.7821 0.5626 0.1516 1973 0.8595 0.6031 0.2728 0.1752 0.1735 0.05436 1974 2.935 2.309 0.9161 0.3775 0.2891 0.08931 1975 0.6436 0.4729 0.2989 0.1819 0.1417 0.04302 1976 1.952 1.575 0.7694 0.491 0.3718 0.1051 1977 12.82 9.263 3.708 1.911 1.313 0.3414 1978 1.153 0.8982 0.4677 0.2664 0.2102 0.05829 1979 15.97 11.25 4.168 1.692 1.171 0.3055 1980 2.552 1.897 0.9808 0.4559 0.3185 0.1049 1981 1.52 1.1 0.4192 0.2293 0.1659 0.06212 1982 5.508 4.181 1.761 0.9306 0.6713 0.1811 1983 2.779 2.069 0.9363 0.4182 0.3237 0.09321 1984 4.42 3.582 1.538 0.7485 0.6248 0.1797 1985 1.235 0.8983 0.3458 0.2724 0.2384 0.0745 1986 3.094 2.492 1.098 0.6035 0.5064 0.1419 1987 1.633 1.174 0.4885 0.2802 0.2285 0.06794 1988 1.8 1.348 0.8686 0.4512 0.3444 0.0945 1989 1.272 0.969 0.5255 0.256 0.1934 0.05668 1990 2.256 1.644 0.6705 0.5025 0.4305 0.1223

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258065 15.97 11.25 4.168 1.911 1.313 0.3414 0.064516129 12.82 9.263 3.708 1.692 1.171 0.3055 0.096774194 5.581 4.181 2.412 1.302 0.8938 0.231 0.129032258 5.508 4.107 2.156 0.9306 0.6713 0.1811 0.161290323 4.495 3.582 1.761 0.8608 0.6248 0.1797 0.193548387 4.42 3.482 1.598 0.7821 0.5881 0.1582 0.225806452 4.28 3.387 1.546 0.7485 0.5626 0.1516 0.258064516 4.026 3.384 1.538 0.7029 0.5064 0.1444 0.290322581 3.431 2.595 1.258 0.6552 0.4866 0.1419 0.322580645 3.094 2.492 1.163 0.6179 0.4678 0.1373 0.35483871 3.02 2.309 1.098 0.6035 0.4305 0.1223 0.387096774 2.935 2.19 0.9808 0.585 0.4274 0.1188 0.419354839 2.779 2.069 0.9595 0.5447 0.4124 0.1168 0.451612903 2.776 1.997 0.9363 0.5025 0.3824 0.1052 0.483870968 2.552 1.897 0.9161 0.491 0.3718 0.1051 0.516129032 2.256 1.801 0.8686 0.4559 0.3444 0.1049

## 87 0.548387097 2.246 1.644 0.8664 0.454 0.3397 0.0945 0.580645161 2.209 1.584 0.8017 0.4512 0.3237 0.09419 0.612903226 1.954 1.575 0.7694 0.4182 0.3185 0.09321 0.64516129 1.952 1.547 0.7635 0.4026 0.2937 0.08931 0.677419355 1.8 1.348 0.6705 0.3949 0.2891 0.08347 0.709677419 1.633 1.174 0.5401 0.3775 0.2755 0.07866 0.741935484 1.52 1.1 0.5255 0.2802 0.2384 0.0745 0.774193548 1.315 0.969 0.4885 0.2724 0.2285 0.06794 0.806451613 1.272 0.953 0.4744 0.2664 0.2102 0.06249 0.838709677 1.235 0.9258 0.4677 0.256 0.1934 0.06212 0.870967742 1.218 0.8983 0.4192 0.2293 0.1735 0.05829 0.903225806 1.153 0.8982 0.3458 0.2172 0.1669 0.05668 0.935483871 0.8595 0.6031 0.2989 0.1819 0.1659 0.05436 0.967741935 0.6436 0.4729 0.2728 0.1752 0.1417 0.04302

0.1 5.5737 4.1736 2.3864 1.26486 0.87155 0.22601 Average of yearly averages: 0.121931333

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: AFLnurs2 Metfile: w12839.dvf PRZM scenario: FLnursery no_irrig.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.90E-05 atm-m^3/mol Vapor Pressure vapr 1.27E-06 torr Solubility sol 0.067 mg/L Kd Kd 27 mg/L Koc Koc mg/L Photolysis half-life kdp 0.19 days Half-life Aerobic Aquatic Metabolism kbacw 97 days Halfife Anaerobic Aquatic Metabolism kbacs 649.8 days Halfife Aerobic Soil Metabolism asm 48.5 days Halfife Hydrolysis: pH 7 9.6 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.134 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 15-03 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 14 days Set to 0 or delete line for single app. Interval 2 interval 14 days Set to 0 or delete line for single app. Interval 3 interval 14 days Set to 0 or delete line for single app. Interval 4 interval 14 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none "none, mont hly or total(average of entire run)"

## 88 

# FL Nursery Plants (Ros es) – Model Approach 1

stored as AFLnurs3.out Chemical: Acequinocyl PRZM environment: FLnursery no_irrig.txt "modi fied Tueday, 17 April 2007 at 08:28:20"

EXAMS environment: pond298.exv "modified Thuday , 29 August 2002 at 17:33:30" Metfile: w12839.dvf "modified Wedday , 3 July 2002 at 09:04:28" Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 0.4037 0.2563 0.1344 0.1007 0.09399 0.0505 1962 0.6482 0.419 0.2417 0.1939 0.1798 0.1169 1963 0.9766 0.6116 0.3249 0.2322 0.211 0.16 1964 1.869 1.108 0.4804 0.3686 0.3533 0.244 1965 1.638 1.026 0.5902 0.4798 0.4409 0.3081 1966 1.763 1.2 0.5914 0.53 0.4962 0.3969 1967 1.904 1.33 0.6688 0.5096 0.4691 0.3865 1968 1.386 0.9534 0.7078 0.6704 0.6209 0.4656 1969 1.069 0.7561 0.5624 0.4593 0.4514 0.4142 1970 1.579 1.003 0.6057 0.4516 0.4155 0.3514 1971 1.038 0.7551 0.4999 0.3892 0.3797 0.3083 1972 1.573 1.105 0.5659 0.473 0.4507 0.3429 1973 1.083 0.7333 0.5316 0.4081 0.3755 0.3061 1974 1.419 0.9195 0.4383 0.3529 0.3482 0.2853 1975 0.581 0.4591 0.3304 0.2759 0.2595 0.2353 1976 1.299 0.8563 0.4536 0.3742 0.355 0.2729 1977 4.418 2.452 0.9088 0.7356 0.653 0.4304 1978 0.6408 0.515 0.3952 0.3728 0.3669 0.3262 1979 5.719 3.089 1.042 0.643 0.5702 0.3918 1980 1.4 0.9144 0.6226 0.5244 0.5043 0.4133 1981 1.823 1.257 0.6499 0.5963 0.5385 0.3868 1982 2.435 1.497 0.6865 0.5725 0.5452 0.4327 1983 1.336 0.929 0.5521 0.4849 0.4709 0.4106 1984 2.166 1.461 0.7375 0.5936 0.5657 0.4415 1985 1.25 0.8668 0.6897 0.5706 0.5523 0.4023 1986 1.357 0.9885 0.5821 0.5087 0.486 0.4037 1987 1.415 0.8662 0.5232 0.4133 0.3938 0.3242 1988 1.13 0.769 0.4954 0.4172 0.4078 0.3095 1989 1.212 0.7905 0.5004 0.3998 0.369 0.2762 1990 1.357 0.8214 0.541 0.4343 0.4195 0.3324

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258065 5.719 3.089 1.042 0.7356 0.653 0.4656 0.064516129 4.418 2.452 0.9088 0.6704 0.6209 0.4415 0.096774194 2.435 1.497 0.7375 0.643 0.5702 0.4327 0.129032258 2.166 1.461 0.7078 0.5963 0.5657 0.4304 0.161290323 1.904 1.33 0.6897 0.5936 0.5523 0.4142 0.193548387 1.869 1.257 0.6865 0.5725 0.5452 0.4133 0.225806452 1.823 1.2 0.6688 0.5706 0.5385 0.4106 0.258064516 1.763 1.108 0.6499 0.53 0.5043 0.4037 0.290322581 1.638 1.105 0.6226 0.5244 0.4962 0.4023 0.322580645 1.579 1.026 0.6057 0.5096 0.486 0.3969 0.35483871 1.573 1.003 0.5914 0.5087 0.4709 0.3918 0.387096774 1.419 0.9885 0.5902 0.4849 0.4691 0.3868 0.419354839 1.415 0.9534 0.5821 0.4798 0.4514 0.3865 0.451612903 1.4 0.929 0.5659 0.473 0.4507 0.3514 0.483870968 1.386 0.9195 0.5624 0.4593 0.4409 0.3429

## 89 0.516129032 1.357 0.9144 0.5521 0.4516 0.4195 0.3324 0.548387097 1.357 0.8668 0.541 0.4343 0.4155 0.3262 0.580645161 1.336 0.8662 0.5316 0.4172 0.4078 0.3242 0.612903226 1.299 0.8563 0.5232 0.4133 0.3938 0.3095 0.64516129 1.25 0.8214 0.5004 0.4081 0.3797 0.3083 0.677419355 1.212 0.7905 0.4999 0.3998 0.3755 0.3081 0.709677419 1.13 0.769 0.4954 0.3892 0.369 0.3061 0.741935484 1.083 0.7561 0.4804 0.3742 0.3669 0.2853 0.774193548 1.069 0.7551 0.4536 0.3728 0.355 0.2762 0.806451613 1.038 0.7333 0.4383 0.3686 0.3533 0.2729 0.838709677 0.9766 0.6116 0.3952 0.3529 0.3482 0.244 0.870967742 0.6482 0.515 0.3304 0.2759 0.2595 0.2353 0.903225806 0.6408 0.4591 0.3249 0.2322 0.211 0.16 0.935483871 0.581 0.419 0.2417 0.1939 0.1798 0.1169 0.967741935 0.4037 0.2563 0.1344 0.1007 0.09399 0.0505

0.1 2.4081 1.4934 0.73453 0.63833 0.56975 0.43247 Average of yearly averages: 0.330883333

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: AFLnurs3 Metfile: w12839.dvf PRZM scenario: FLnursery no_irrig.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.90E-05 atm-m^3/mol Vapor Pressure vapr 1.27E-06 torr Solubility sol 0.067 mg/L Kd Kd 678 mg/L Koc Koc mg/L Photolysis half-life kdp 0 days Half-life Aerobic Aquatic Metabolism kbacw 0 days Halfife Anaerobic Aquatic Metabolism kbacs 0 days Halfife Aerobic Soil Metabolism asm 0 days Halfife Hydrolysis: pH 7 0 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.067 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 15-03 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 14 days Set to 0 or delete line for single app. Interval 2 interval 14 days Set to 0 or delete line for single app. Interval 3 interval 14 days Set to 0 or delete line for single app. Interval 4 interval 14 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none "none, mont hly or total(average of entire run)"

## 90 

# FL Nursery Plants (Ros es) – Model Approach 2

stored as AFLnurs4.out Chemical: Acequinocyl PRZM environment: FLnursery no_irrig.txt "modi fied Tueday, 17 April 2007 at 08:28:20"

EXAMS environment: pond298.exv "modified Thuday , 29 August 2002 at 17:33:30" Metfile: w12839.dvf "modified Wedday , 3 July 2002 at 09:04:28" Water segment concentrations (ppb)

Year Peak 96 hr 21 Day 60 Day 90 Day Yearly 1961 1.51 1.095 0.6289 0.309 0.2137 0.05939 1962 0.9769 0.7736 0.4797 0.2013 0.1469 0.04173 1963 1.388 0.9987 0.3818 0.1974 0.1377 0.03933 1964 1.123 0.9005 0.4331 0.2925 0.2062 0.05837 1965 0.6576 0.4766 0.2372 0.1086 0.08346 0.03124 1966 1.716 1.298 0.5817 0.3515 0.2433 0.07219 1967 2.247 1.741 0.7989 0.3276 0.2339 0.06864 1968 2.139 1.693 1.206 0.6512 0.4469 0.1155 1969 0.6089 0.4627 0.27 0.227 0.1698 0.04709 1970 2.791 2.054 1.078 0.4304 0.294 0.07911 1971 1.105 0.7921 0.4008 0.2724 0.1912 0.05262 1972 2.013 1.692 0.7727 0.391 0.2813 0.07581 1973 0.4297 0.3015 0.1364 0.08759 0.08676 0.02718 1974 1.468 1.155 0.4581 0.1888 0.1446 0.04466 1975 0.3219 0.2365 0.1495 0.09098 0.07087 0.02151 1976 0.9758 0.7874 0.3846 0.2455 0.1859 0.05255 1977 6.412 4.631 1.854 0.9555 0.6564 0.1707 1978 0.5762 0.449 0.2338 0.1332 0.1051 0.02914 1979 7.981 5.625 2.083 0.846 0.5851 0.1527 1980 1.276 0.9486 0.4904 0.2279 0.1592 0.05243 1981 0.7601 0.5503 0.2097 0.1147 0.08297 0.03107 1982 2.754 2.091 0.8807 0.4653 0.3357 0.09054 1983 1.389 1.034 0.468 0.209 0.1618 0.0466 1984 2.21 1.791 0.7691 0.3742 0.3124 0.08983 1985 0.6173 0.449 0.1728 0.1362 0.1192 0.03725 1986 1.547 1.246 0.549 0.3018 0.2532 0.07094 1987 0.8164 0.5867 0.2442 0.1401 0.1142 0.03397 1988 0.9003 0.6741 0.4343 0.2256 0.1722 0.04725 1989 0.6357 0.4845 0.2628 0.128 0.09671 0.02834 1990 1.128 0.8222 0.3353 0.2513 0.2153 0.06115

Sorted results Prob. Peak 96 hr 21 Day 60 Day 90 Day Yearly 0.032258065 7.981 5.625 2.083 0.9555 0.6564 0.1707 0.064516129 6.412 4.631 1.854 0.846 0.5851 0.1527 0.096774194 2.791 2.091 1.206 0.6512 0.4469 0.1155 0.129032258 2.754 2.054 1.078 0.4653 0.3357 0.09054 0.161290323 2.247 1.791 0.8807 0.4304 0.3124 0.08983 0.193548387 2.21 1.741 0.7989 0.391 0.294 0.07911 0.225806452 2.139 1.693 0.7727 0.3742 0.2813 0.07581 0.258064516 2.013 1.692 0.7691 0.3515 0.2532 0.07219 0.290322581 1.716 1.298 0.6289 0.3276 0.2433 0.07094 0.322580645 1.547 1.246 0.5817 0.309 0.2339 0.06864 0.35483871 1.51 1.155 0.549 0.3018 0.2153 0.06115 0.387096774 1.468 1.095 0.4904 0.2925 0.2137 0.05939 0.419354839 1.389 1.034 0.4797 0.2724 0.2062 0.05837 0.451612903 1.388 0.9987 0.468 0.2513 0.1912 0.05262 0.483870968 1.276 0.9486 0.4581 0.2455 0.1859 0.05255 0.516129032 1.128 0.9005 0.4343 0.2279 0.1722 0.05243

## 91 0.548387097 1.123 0.8222 0.4331 0.227 0.1698 0.04725 0.580645161 1.105 0.7921 0.4008 0.2256 0.1618 0.04709 0.612903226 0.9769 0.7874 0.3846 0.209 0.1592 0.0466 0.64516129 0.9758 0.7736 0.3818 0.2013 0.1469 0.04466 0.677419355 0.9003 0.6741 0.3353 0.1974 0.1446 0.04173 0.709677419 0.8164 0.5867 0.27 0.1888 0.1377 0.03933 0.741935484 0.7601 0.5503 0.2628 0.1401 0.1192 0.03725 0.774193548 0.6576 0.4845 0.2442 0.1362 0.1142 0.03397 0.806451613 0.6357 0.4766 0.2372 0.1332 0.1051 0.03124 0.838709677 0.6173 0.4627 0.2338 0.128 0.09671 0.03107 0.870967742 0.6089 0.449 0.2097 0.1147 0.08676 0.02914 0.903225806 0.5762 0.449 0.1728 0.1086 0.08346 0.02834 0.935483871 0.4297 0.3015 0.1495 0.09098 0.08297 0.02718 0.967741935 0.3219 0.2365 0.1364 0.08759 0.07087 0.02151

0.1 2.7873 2.0873 1.1932 0.63261 0.43578 0.113004 Average of yearly averages: 0.060961

Inputs generated by pe4.pl - 8-August-2003

Data used for this run: Output File: AFLnurs4 Metfile: w12839.dvf PRZM scenario: FLnursery no_irrig.txt EXAMS environment file: pond298.exv Chemical Name: Acequinocyl Description Variable Na me Value Units Comments Molecular weight mwt 384.5 g/mol Henry's Law Const. henr y 3.90E-05 atm-m^3/mol Vapor Pressure vapr 1.27E-06 torr Solubility sol 0.067 mg/L Kd Kd 27 mg/L Koc Koc mg/L Photolysis half-life kdp 0.19 days Half-life Aerobic Aquatic Metabolism kbacw 97 days Halfife Anaerobic Aquatic Metabolism kbacs 649.8 days Halfife Aerobic Soil Metabolism asm 48.5 days Halfife Hydrolysis: pH 7 9.6 days Half-life Method: CAM 2 integer See PRZM manual Incorporation Depth: DEPI 0 cm Application Rate: TAPP 0.067 kg/ha Application Efficiency: APPEFF 0.99 fraction Spray Drift DRFT 0.01 fraction of application rate applied to pond Application Date Date 15-03 dd/mm or dd/mmm or dd-mm or dd-mmm Interval 1 interval 14 days Set to 0 or delete line for single app. Interval 2 interval 14 days Set to 0 or delete line for single app. Interval 3 interval 14 days Set to 0 or delete line for single app. Interval 4 interval 14 days Set to 0 or delete line for single app. Record 17: FILTRA IPSCND 1 UPTKF Record 18: PLVKRT PLDKRT FEXTRC 0.5 Flag for Index Res. Run IR Pond Flag for runoff calc. RUNOFF none "none, mont hly or total(average of entire run)"

## 92 

# Appendix B. Terrestrial Exposure TR EX Inputs for Food Uses, Non-Food Uses (Except Roses), and Non-Food Uses (Roses) Using 2 Half-life Periods (35 and 20 days)

Chemical Name: Acequinocyl

Use Food uses

Formulation 15.8 SC Application Rate 0.3 lbs ai/acre

Half-life 35 days Application Interval 21 days Maximum # Apps./Year 2 Length of Simulation 1 year

Endpoints

Bobwhite quail LD50 (mg/kg-bw) 2250.00 Mallard duck) LC50 (mg/kg-diet) 5000.00 Bobwhite quail NOAEL(mg/kg-bw) 0.00 Avian

Mallard duc k NOAEC (mg/kg-diet) 100.00 LD50 (mg/kg-bw) 5000.00 LC50 (mg/kg-diet) 0.00 NOAEL (mg/kg-bw) 5.00 Mammals

NOAEC (mg/kg-diet) 100.00

Chemical Name: Acequinocyl

Use Food uses

Formulation 15.8 SC Application Rate 0.3 lbs ai/acre

Half-life 20 days Application Interval 21 days Maximum # Apps./Year 2 Length of Simulation 1 year

Endpoints

Bobwhite quail LD50 (mg/kg-bw) 2250.00 Mallard duck) LC50 (mg/kg-diet) 5000.00 Bobwhite quail NOAEL(mg/kg-bw) 0.00 Avian

Mallard duck NOAEC (mg/kg-diet) 100.00 LD50 (mg/kg-bw) 5000.00 LC50 (mg/kg-diet) 0.00 Mammals NOAEL (mg/kg-bw) 5.00

## 93 Chemical Name: Acequinocyl

Use Non-food uses

Formulation 15.8 SC Application Rate 0.125 lbs ai/acre

Half-life 35 days Application Interval 14 days Maximum # Apps./Year 5 Length of Simulation 1 year

Endpoints

Bobwhite quail LD50 (mg/kg-bw) 2250.00 Mallard duck) LC50 (mg/kg-diet) 5000.00 Bobwhite quail NOAEL(mg/kg-bw) 0.00 Avian

Mallard duck NOAEC (mg/kg-diet) 100.00

LD50 (mg/kg-bw) 5000.00 LC50 (mg/kg-diet) 0.00 NOAEL (mg/kg-bw) 5.00 Mammals

NOAEC (mg/kg-diet) 100.00

Chemical Name: Acequinocyl

Use Non-food uses

Formulation 15.8 SC Application Rate 0.125 lbs ai/acre

Half-life 20 days Application Interval 14 days Maximum # Apps./Year 5 Length of Simulation 1 year

Endpoints

Bobwhite quail LD50 (mg/kg-bw) 2250.00 Mallard duck) LC50 (mg/kg-diet) 5000.00 Bobwhite quail NOAEL(mg/kg-bw) 0.00 Avian

Mallard duck NOAEC (mg/kg-diet) 100.00

LD50 (mg/kg-bw) 5000.00 LC50 (mg/kg-diet) 0.00 NOAEL (mg/kg-bw) 5.00 Mammals

NOAEC (mg/kg-diet) 100.00

## 94 Chemical Name: Acequinocyl

Use Non-food uses (roses)

Formulation 15.8 SC Application Rate 0.06 lbs ai/acre

Half-life 35 days Application Interval 14 days Maximum # Apps./Year 5 Length of Simulation 1 year

Endpoints

Bobwhite quail LD50 (mg/kg-bw) 2250.00 Mallard duck) LC50 (mg/kg-diet) 5000.00 Bobwhite quail NOAEL(mg/kg-bw) 0.00 Avian

Mallard duck NOAEC (mg/kg-diet) 100.00

LD50 (mg/kg-bw) 5000.00 LC50 (mg/kg-diet) 0.00 NOAEL (mg/kg-bw) 5.00 Mammals

NOAEC (mg/kg-diet) 100.00

Chemical Name: Acequinocyl

Use Non-food uses (roses)

Formulation 15.8 SC Application Rate 0.06 lbs ai/acre

Half-life 20 days Application Interval 14 days Maximum # Apps./Year 5 Length of Simulation 1 year

Endpoints

Bobwhite quail LD50 (mg/kg-bw) 2250.00 Mallard duck) LC50 (mg/kg-diet) 5000.00 Bobwhite quail NOAEL(mg/kg-bw) 0.00 Avian

Mallard duck NOAEC (mg/kg-diet) 100.00

LD50 (mg/kg-bw) 5000.00 LC50 (mg/kg-diet) 0.00 NOAEL (mg/kg-bw) 5.00 Mammals

NOAEC (mg/kg-diet) 100.00

## 95 

# Appendix C. Terrestrial Exposure TREX Outputs

Summary of Risk Quotient Calculatio ns Based on Upper Bound Kenaga EECs and Foliar Dissipation Half-Life of 35 Days for Food Uses

Table X. Upper 90th Percentile Kenaga, Ac ute Avian Dose-Based Risk Quotients

EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects Size Class (gras) Adjusted LD50

EEC RQ EEC RQ EEC RQ EEC RQ

20 1620.97 136.10 0.08 62.38 0.04 76.56 0.05 8.51 0.01

100 2063.57 77.61 0.04 35.57 0.02 43.66 0.02 4.85 0.00

1000 2914.87 34.75 0.01 15.93 0.01 19.55 0.01 2.17 0.00

Table X. Upper 90th Percentile Kena ga, Subacute Avian Di etary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects

LC50 EEC RQ EEC RQ EEC RQ EEC RQ 5000 119.50 0.02 54.77 0.01 67.22 0.01 7.47 0.00 Size class not used for dietary risk quotients

Table X. Upper 90th Percentile Kena ga, Chronic Avian Dietary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects NOAEC (ppm) EEC RQ EEC RQ EEC RQ EEC RQ

100 119.50 1.20 54.77 0.55 67.22 0.67 7.47 0.07 Size class not used for dietary risk quotients

## 96 Table X. Upper 90th Percentile Kenaga, Acute Mammalian Dose-Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects Granivore Size Class (grams) Adjusted LD50

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ 15 10989.15 113.94 0.01 52.22 0.00 64.09 0.01 7.12 0.00 1.58 0.00 35 8891.40 78.75 0.01 36.09 0.00 44.29 0.00 4.92 0.00 1.09 0.00 1000 3845.80 18.26 0.00 8.37 0.00 10.27 0.00 1.14 0.00 0.25 0.00

Table X. Upper 90th Percentile Kena ga, Acute Mammalian Dietary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects LC50 (ppm) EEC RQ EEC RQ EEC RQ EEC RQ

0 119.50 ##### 54.77 ##### 67.22 ##### 7.47 #####

Size class not used for dietary risk quotients

Table X. Upper 90th Percentile Kena ga, Chronic Mammalian Dietary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects NOAEC (ppm)

EEC RQ EEC RQ EEC RQ EEC RQ 100 119.50 1.20 54.77 0.55 67.22 0.67 7.47 0.07 Size class not used for dietary risk quotients

Table X. Upper 90th Percentile Kenaga, Ch ronic Mammalian Dose-B ased Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects Granivore Size Class (grams) Adjusted NOAEL

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ 15 10.99 113.94 10.37 52.22 4.75 64.09 5.83 7.12 0.65 1.58 0.14 35 8.89 78.75 8.86 36.09 4.06 44.29 4.98 4.92 0.55 1.09 0.12 1000 3.85 18.26 4.75 8.37 2.18 10.27 2.67 1.14 0.30 0.25 0.07

## 97 Summary of Risk Quotient Calculatio ns Based on Upper Bound Kenaga EECs and Foliar Dissipation Half-Life of 35 Days for Non-Food Uses (Except Roses)

Table X. Upper 90th Percentile Kenaga, Ac ute Avian Dose-Based Risk Quotients

EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects Size Class (grams) Adjusted LD50

EEC RQ EEC RQ EEC RQ EEC RQ

20 1620.97 105.83 0.07 48.50 0.03 59.53 0.04 6.61 0.00

100 2063.57 60.35 0.03 27.66 0.01 33.95 0.02 3.77 0.00

1000 2914.87 27.02 0.01 12.38 0.00 15.20 0.01 1.69 0.00

Table X. Upper 90th Percentile Kena ga, Subacute Avian Di etary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects

LC50 EEC RQ EEC RQ EEC RQ EEC RQ 5000 92.92 0.02 42.59 0.01 52.27 0.01 5.81 0.00 Size class not used for dietary risk quotients

Table X. Upper 90th Percentile Kena ga, Chronic Avian Dietary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects NOAEC (ppm) EEC RQ EEC RQ EEC RQ EEC RQ

100 92.92 0.93 42.59 0.43 52.27 0.52 5.81 0.06 Size class not used for dietary risk quotients

## 98 Table X. Upper 90th Percentile Kenaga, Acute Mammalian Dose-Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects Granivore Size Class (grams) Adjusted LD50

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ 15 10989.15 88.59 0.01 40.61 0.00 49.83 0.00 5.54 0.00 1.23 0.00 35 8891.40 61.23 0.01 28.06 0.00 34.44 0.00 3.83 0.00 0.85 0.00 1000 3845.80 14.20 0.00 6.51 0.00 7.99 0.00 0.89 0.00 0.20 0.00

Table X. Upper 90th Percentile Kena ga, Acute Mammalian Dietary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects LC50 (ppm) EEC RQ EEC RQ EEC RQ EEC RQ

0 92.92 ##### 42.59 ##### 52.27 ##### 5.81 #####

Size class not used for dietary risk quotients

Table X. Upper 90th Percentile Kena ga, Chronic Mammalian Dietary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects NOAEC (ppm)

EEC RQ EEC RQ EEC RQ EEC RQ 100 92.92 0.93 42.59 0.43 52.27 0.52 5.81 0.06 Size class not used for dietary risk quotients

Table X. Upper 90th Percentile Kenaga, Ch ronic Mammalian Dose-B ased Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects Granivore Size Class (grams) Adjusted NOAEL

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ 15 10.99 88.59 8.06 40.61 3.70 49.83 4.53 5.54 0.50 1.23 0.11 35 8.89 61.23 6.89 28.06 3.16 34.44 3.87 3.83 0.43 0.85 0.10 1000 3.85 14.20 3.69 6.51 1.69 7.99 2.08 0.89 0.23 0.20 0.05

## 99 Summary of Risk Quotient Calculatio ns Based on Upper Bound Kenaga EECs and Foliar Dissipation Half-Life of 35 Days for Roses

Table X. Upper 90th Percentile Kenaga, Ac ute Avian Dose-Based Risk Quotients

EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects Size Class (grams) Adjusted LD50

EEC RQ EEC RQ EEC RQ EEC RQ

20 1620.97 50.80 0.03 23.28 0.01 28.57 0.02 3.17 0.00

100 2063.57 28.97 0.01 13.28 0.01 16.29 0.01 1.81 0.00

1000 2914.87 12.97 0.00 5.94 0.00 7.29 0.00 0.81 0.00

Table X. Upper 90th Percentile Kena ga, Subacute Avian Di etary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects

LC50 EEC RQ EEC RQ EEC RQ EEC RQ 5000 44.60 0.01 20.44 0.00 25.09 0.01 2.79 0.00 Size class not used for dietary risk quotients

Table X. Upper 90th Percentile Kena ga, Chronic Avian Dietary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects NOAEC (ppm) EEC RQ EEC RQ EEC RQ EEC RQ

100 44.60 0.45 20.44 0.20 25.09 0.25 2.79 0.03 Size class not used for dietary risk quotients

## 100 Table X. Upper 90th Percentile Kenaga, Acute Mammalian Dose-Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects Granivore Size Class (grams) Adjusted LD50

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ 15 10989.15 42.52 0.00 19.49 0.00 23.92 0.00 2.66 0.00 0.59 0.00 35 8891.40 29.39 0.00 13.47 0.00 16.53 0.00 1.84 0.00 0.41 0.00 1000 3845.80 6.81 0.00 3.12 0.00 3.83 0.00 0.43 0.00 0.09 0.00

Table X. Upper 90th Percentile Kena ga, Acute Mammalian Dietary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects LC50 (ppm) EEC RQ EEC RQ EEC RQ EEC RQ

0 44.60 ##### 20.44 ##### 25.09 ##### 2.79 #####

Size class not used for dietary risk quotients

Table X. Upper 90th Percentile Kena ga, Chronic Mammalian Dietary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects NOAEC (ppm)

EEC RQ EEC RQ EEC RQ EEC RQ 100 44.60 0.45 20.44 0.20 25.09 0.25 2.79 0.03 Size class not used for dietary risk quotients

Table X. Upper 90th Percentile Kenaga, Ch ronic Mammalian Dose-B ased Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects Granivore Size Class (grams) Adjusted NOAEL

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ 15 10.99 42.52 3.87 19.49 1.77 23.92 2.18 2.66 0.24 0.59 0.05 35 8.89 29.39 3.31 13.47 1.52 16.53 1.86 1.84 0.21 0.41 0.05 1000 3.85 6.81 1.77 3.12 0.81 3.83 1.00 0.43 0.11 0.09 0.02

## 101 Summary of Risk Quotient Calculatio ns Based on Upper Bound Kenaga EECs and Foliar Dissipation Half-Life of 20 Days for Food Uses

Table X. Upper 90th Percentile Kenaga, Ac ute Avian Dose-Based Risk Quotients

EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects Size Class (grams) Adjusted LD50

EEC RQ EEC RQ EEC RQ EEC RQ

20 1620.97 121.60 0.08 55.74 0.03 68.40 0.04 7.60 0.00

100 2063.57 69.34 0.03 31.78 0.02 39.01 0.02 4.33 0.00

1000 2914.87 31.05 0.01 14.23 0.00 17.46 0.01 1.94 0.00

Table X. Upper 90th Percentile Kena ga, Subacute Avian Di etary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects

LC50 EEC RQ EEC RQ EEC RQ EEC RQ 5000 106.77 0.02 48.94 0.01 60.06 0.01 6.67 0.00 Size class not used for dietary risk quotients

Table X. Upper 90th Percentile Kena ga, Chronic Avian Dietary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects NOAEC (ppm) EEC RQ EEC RQ EEC RQ EEC RQ

100 106.77 1.07 48.94 0.49 60.06 0.60 6.67 0.07 Size class not used for dietary risk quotients

## 102 Table X. Upper 90th Percentile Kenaga, Acute Mammalian Dose-Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects Granivore Size Class (grams) Adjusted LD50

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ 15 10989.15 101.80 0.01 46.66 0.00 57.26 0.01 6.36 0.00 1.41 0.00 35 8891.40 70.36 0.01 32.25 0.00 39.58 0.00 4.40 0.00 0.98 0.00 1000 3845.80 16.31 0.00 7.48 0.00 9.18 0.00 1.02 0.00 0.23 0.00

Table X. Upper 90th Percentile Kena ga, Acute Mammalian Dietary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects LC50 (ppm) EEC RQ EEC RQ EEC RQ EEC RQ

0 106.77 ##### 48.94 ##### 60.06 ##### 6.67 #####

Size class not used for dietary risk quotients

Table X. Upper 90th Percentile Kena ga, Chronic Mammalian Dietary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects NOAEC (ppm)

EEC RQ EEC RQ EEC RQ EEC RQ 100 106.77 1.07 48.94 0.49 60.06 0.60 6.67 0.07 Size class not used for dietary risk quotients

Table X. Upper 90th Percentile Kenaga, Ch ronic Mammalian Dose-B ased Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects Granivore Size Class (grams) Adjusted NOAEL

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ 15 10.99 101.80 9.26 46.66 4.25 57.26 5.21 6.36 0.58 1.41 0.13 35 8.89 70.36 7.91 32.25 3.63 39.58 4.45 4.40 0.49 0.98 0.11 1000 3.85 16.31 4.24 7.48 1.94 9.18 2.39 1.02 0.27 0.23 0.06

## 103 Summary of Risk Quotient Calculatio ns Based on Upper Bound Kenaga EECs and Foliar Dissipation Half-Life of 20 Days for Non-Food Uses (Except Roses)

Table X. Upper 90th Percentile Kenaga, Ac ute Avian Dose-Based Risk Quotients

EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects Size Class (grams) Adjusted LD50

EEC RQ EEC RQ EEC RQ EEC RQ

20 1620.97 81.02 0.05 37.13 0.02 45.57 0.03 5.06 0.00

100 2063.57 46.20 0.02 21.18 0.01 25.99 0.01 2.89 0.00

1000 2914.87 20.69 0.01 9.48 0.00 11.64 0.00 1.29 0.00

Table X. Upper 90th Percentile Kena ga, Subacute Avian Di etary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects

LC50 EEC RQ EEC RQ EEC RQ EEC RQ 5000 71.14 0.01 32.61 0.01 40.02 0.01 4.45 0.00 Size class not used for dietary risk quotients

Table X. Upper 90th Percentile Kena ga, Chronic Avian Dietary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects NOAEC (ppm) EEC RQ EEC RQ EEC RQ EEC RQ

100 71.14 0.71 32.61 0.33 40.02 0.40 4.45 0.04 Size class not used for dietary risk quotients

## 104 Table X. Upper 90th Percentile Kenaga, Acute Mammalian Dose-Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects Granivore Size Class (grams) Adjusted LD50

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ 15 10989.15 67.83 0.01 31.09 0.00 38.15 0.00 4.24 0.00 0.94 0.00 35 8891.40 46.88 0.01 21.49 0.00 26.37 0.00 2.93 0.00 0.65 0.00 1000 3845.80 10.87 0.00 4.98 0.00 6.11 0.00 0.68 0.00 0.15 0.00

Table X. Upper 90th Percentile Kena ga, Acute Mammalian Dietary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects LC50 (ppm) EEC RQ EEC RQ EEC RQ EEC RQ

0 71.14 ##### 32.61 ##### 40.02 ##### 4.45 #####

Size class not used for dietary risk quotients

Table X. Upper 90th Percentile Kena ga, Chronic Mammalian Dietary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects NOAEC (ppm)

EEC RQ EEC RQ EEC RQ EEC RQ 100 71.14 0.71 32.61 0.33 40.02 0.40 4.45 0.04 Size class not used for dietary risk quotients

Table X. Upper 90th Percentile Kenaga, Ch ronic Mammalian Dose-B ased Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects Granivore Size Class (grams) Adjusted NOAEL

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ 15 10.99 67.83 6.17 31.09 2.83 38.15 3.47 4.24 0.39 0.94 0.09 35 8.89 46.88 5.27 21.49 2.42 26.37 2.97 2.93 0.33 0.65 0.07 1000 3.85 10.87 2.83 4.98 1.30 6.11 1.59 0.68 0.18 0.15 0.04

## 105 Summary of Risk Quotient Calculatio ns Based on Upper Bound Kenaga EECs and Foliar Dissipation Half-Life of 20 Days for Roses

Table X. Upper 90th Percentile Kenaga, Ac ute Avian Dose-Based Risk Quotients

EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects Size Class (grams) Adjusted LD50

EEC RQ EEC RQ EEC RQ EEC RQ

20 1620.97 38.89 0.02 17.82 0.01 21.88 0.01 2.43 0.00

100 2063.57 22.18 0.01 10.16 0.00 12.47 0.01 1.39 0.00

1000 2914.87 9.93 0.00 4.55 0.00 5.59 0.00 0.62 0.00

Table X. Upper 90th Percentile Kena ga, Subacute Avian Di etary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects

LC50 EEC RQ EEC RQ EEC RQ EEC RQ 5000 34.15 0.01 15.65 0.00 19.21 0.00 2.13 0.00 Size class not used for dietary risk quotients

Table X. Upper 90th Percentile Kena ga, Chronic Avian Dietary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects NOAEC (ppm) EEC RQ EEC RQ EEC RQ EEC RQ

100 34.15 0.34 15.65 0.16 19.21 0.19 2.13 0.02 Size class not used for dietary risk quotients

## 106 Table X. Upper 90th Percentile Kenaga, Acute Mammalian Dose-Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects Granivore Size Class (grams) Adjusted LD50

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ 15 10989.15 32.56 0.00 14.92 0.00 18.31 0.00 2.03 0.00 0.45 0.00 35 8891.40 22.50 0.00 10.31 0.00 12.66 0.00 1.41 0.00 0.31 0.00 1000 3845.80 5.22 0.00 2.39 0.00 2.93 0.00 0.33 0.00 0.07 0.00

Table X. Upper 90th Percentile Kena ga, Acute Mammalian Dietary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects LC50 (ppm) EEC RQ EEC RQ EEC RQ EEC RQ

0 34.15 ##### 15.65 ##### 19.21 ##### 2.13 #####

Size class not used for dietary risk quotients

Table X. Upper 90th Percentile Kena ga, Chronic Mammalian Dietary Based Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects NOAEC (ppm)

EEC RQ EEC RQ EEC RQ EEC RQ 100 34.15 0.34 15.65 0.16 19.21 0.19 2.13 0.02 Size class not used for dietary risk quotients

Table X. Upper 90th Percentile Kenaga, Ch ronic Mammalian Dose-B ased Risk Quotients EECs and RQs

Short Grass Tall Grass Broadleaf Plants/ Small Insects Fruits/Pods/ Seeds/ Large Insects Granivore Size Class (grams) Adjusted NOAEL

EEC RQ EEC RQ EEC RQ EEC RQ EEC RQ 15 10.99 32.56 2.96 14.92 1.36 18.31 1.67 2.03 0.19 0.45 0.04 35 8.89 22.50 2.53 10.31 1.16 12.66 1.42 1.41 0.16 0.31 0.04 1000 3.85 5.22 1.36 2.39 0.62 2.93 0.76 0.33 0.08 0.07 0.02

## 107 

# Appendix D. Terrestrial Plant Expo sure TERRPLANT Inputs and Outputs

Table 1. Chemical Identity.

Chemical Name Acequinocyl PC code 6329 Use Insecticide (miticide) Application Method Foliar spray

Application Form Liquid Solubility in Water (ppm) 0.0067

Table 2. Input parameters used to derive EECs. Input Parameter Symbol Value Units Application Rate A 0.3 (food uses) y

Incorporation I 1 none Runoff Fraction R 0.01 none

Drift Fraction D 0.01 none

Table 3. EECs for Acequinocyl. Units in y. Description Equation EEC Runoff to dry areas (A/I)*R 0.003 Runoff to semi-aquatic areas (A/I)*R*10 0.03 Spray drift A*D 0.003 Total for dry areas ((A/I)*R)+(A*D) 0.006 Total for semi-aquatic areas ((A/I)*R*10)+(A*D) 0.033

Table 4. Plant survival and growth data used for RQ derivation. Units are in y. Seedling Emergence Vegetative Vigor Plant type EC 25 

### NOAEC EC 25 

### NOAEC

Monocot 0.9 0.9 0.9 0.9

Dicot 0.7 0.3 0.9 0.3

Table 5. RQ values for plants in dry and semi-aquatic areas exposed to Acequinocyl through runoff and/or spray drift.* Plant Type Listed Status Dry Semi-Aquatic Spray Drift

Monocot non-listed <0.1 <0.1 <0.1 Monocot listed <0.1 <0.1 <0.1

Dicot non-listed <0.1 <0.1 <0.1 Dicot listed <0.1 0.11 <0.1 *If RQ > 1.0, the LOC is exceeded, resulti ng in potential for risk to that plant group

## 108 Table 1. Chemical Identity.

Chemical Name Acequinocyl PC code 6329 Use Insecticide (miticide) Application Method Foliar spray

Application Form Liquid Solubility in Water (ppm) 0.0067

Table 2. Input parameters used to derive EECs. Input Parameter Symbol Value Units

Application Rate A 0.125 (non-food uses – except roses) y Incorporation I 1 none Runoff Fraction R 0.01 none

Drift Fraction D 0.01 none

Table 3. EECs for Acequinocyl. Units in y. Description Equation EEC Runoff to dry areas (A/I)*R 0.00125 Runoff to semi-aquatic areas (A/I)*R*10 0.0125 Spray drift A*D 0.00125 Total for dry areas ((A/I)*R)+(A*D) 0.0025 Total for semi-aquatic areas ((A/I)*R*10)+(A*D) 0.01375

Table 4. Plant survival and growth data used for RQ derivation. Units are in y. Seedling Emergence Vegetative Vigor Plant type EC 25 

### NOAEC EC 25 

### NOAEC

Monocot 0.9 0.9 0.9 0.9

Dicot 0.7 0.3 0.9 0.3

Table 5. RQ values for plants in dry and semi-aquatic areas exposed to Acequinocyl through runoff and/or spray drift.* Plant Type Listed Status Dry Semi-Aquatic Spray Drift

Monocot non-listed <0.1 <0.1 <0.1 Monocot listed <0.1 <0.1 <0.1

Dicot non-listed <0.1 <0.1 <0.1 Dicot listed <0.1 <0.1 <0.1 *If RQ > 1.0, the LOC is exceeded, resulti ng in potential for risk to that plant group

## 109 Table 1. Chemical Identity.

Chemical Name Acequinocyl PC code 6329 Use Insecticide (miticide) Application Method Foliar spray

Application Form Liquid Solubility in Water (ppm) 0.0067

Table 2. Input parameters used to derive EECs. Input Parameter Symbol Value Units

Application Rate A 0.06 (non-food uses- roses) y Incorporation I 1 none Runoff Fraction R 0.01 none

Drift Fraction D 0.01 none

Table 3. EECs for Acequinocyl. Units in y. Description Equation EEC Runoff to dry areas (A/I)*R 0.0006 Runoff to semi-aquatic areas (A/I)*R*10 0.006 Spray drift A*D 0.0006 Total for dry areas ((A/I)*R)+(A*D) 0.0012 Total for semi-aquatic areas ((A/I)*R*10)+(A*D) 0.0066

Table 4. Plant survival and growth data used for RQ derivation. Units are in y. Seedling Emergence Vegetative Vigor Plant type EC 25 

### NOAEC EC 25 

### NOAEC

Monocot 0.9 0.9 0.9 0.9

Dicot 0.7 0.3 0.9 0.3

Table 5. RQ values for plants in dry and semi-aquatic areas exposed to Acequinocyl through runoff and/or spray drift.* Plant Type Listed Status Dry Semi-Aquatic Spray Drift

Monocot non-listed <0.1 <0.1 <0.1 Monocot listed <0.1 <0.1 <0.1

Dicot non-listed <0.1 <0.1 <0.1 Dicot listed <0.1 <0.1 <0.1 *If RQ > 1.0, the LOC is exceeded, resulti ng in potential for risk to that plant group.

## 110 

# Appendix E. Ecological Studies Submitted for Acequinocyl

Study Guideline Data Requirement MRID # Study Classification

Birds

71-1a Avian acute oral LD 50 , Japanese quail 45435002 Supplemental 1

71-1a Avian acute oral LD 50 , Mallard duck 45435001 Invalid 4

71-1b Avian acute oral LD 50 , Bobwhite quail( (Formulation) 45530602 Acceptable

71-2a Avian subacute dietary LC 50 , Bobwhite quail, (Formulation) 45530603 Invalid

71-2a Avian subacute dietary LC 50, Japanese quail 45435004 Supplemental 1

71-2b Avian subacute dietary LC 50, Mallard Duck 45435003 Acceptable

71-4a Avian Reproduction, Bobwhite quail 45435006 Acceptable

71-4b Avian Reproduction, Mallard duck 45435005 Acceptable

850-2100 Avian acute oral LD50, Bobw hite quail 46615501 Acceptable 4

850-2200 Avian dietary LC50, Bobwhite quail 46622701 Acceptable 4



### Fish and Invertebrates

72-1a Freshwater fish acute LC 50 /EC 50 , Bluegill sunfish 45434918 Supplemental 2

72-1b Freshwater fish acute LC 50 /EC 50 , Bluegill sunfish (Formulation) 45428605 Acceptable

72-1c Freshwater fish acute LC 50 /EC 50 , Rainbow trout 45434920 and 45868502 Supplemental 2 (Both studies)

72-1d Freshwater fish acute LC 50 /EC 50 , Rainbow trout (Formulation) 45530601 Acceptable

72-1 Freshwater fish acute LC 50 /EC 50 , Zebrafish 45434919 Supplemental 1, 2

850-1075 Freshwater fish acute LC50, common carp (R1 degradate) 46370502 Supplemental 4

72-2a Freshwater invertebrate, Daphnia EC 50 45434915 Invalid 4

72-2a Freshwater invertebrate, Chironomid LC 50 (Formulation) 45782303 Supplemental 3

72-3a Estuarine/marine fish acute LC 50 , Sheepshead minnow 45434921 Supplemental 2

72-3d Estuarine/marine fish acute LC 50 , Sheepshead minnow (Formulation) 45428606 Acceptable

72-3b Estuarine/marine acute invertebrate EC 50 , Eastern oyster 45434916 Supplemental 2

72-3c Estuarine/marine acute invertebrate EC 50 , Mysid shrimp 45434917 Supplemental 2

72-4a Freshwater early life stage chronic, Rainbow trout (Formulation) 45428607 Supplemental

72-4b Freshwater invertebrate life cycle, Daphnia 45434922 Supplemental

72-4c Estuarine/marine fish early life-stage, Sheepshead minnow No study N/A

## 111 72-4b Estuarine/marine life cycle, Mysid No study N/A 4

850-1010 Freshwater invertebrate LC 50 , Daphnia 46370502 Invalid 4

850-1010 Freshwater invertebrate LC 50 , Daphnia 46372101 Acceptable 4



### Mammals

81-1 Acute mammalian oral LD 50 (Rat) 45435011 Acceptable

81-1 Acute mammalian oral LD 50 (Mouse) 45435010 Acceptable

83-3a Mammalian Developmental – Rat 45531908 and 45651706 Acceptable

83-3b Mammalian Developmental – Rabbit 45531907 and 45651705 Acceptable

83-4 2-Generation Reproduction Study - Rat 45531909 Acceptable

Plants

122-1a Terrestrial plants (Tier I) - Seedling Emergence (Formulation) 45428609 Acceptable

123-1b Terrestrial plants (Tier I) -V egetative Vigor (Formulation) 45428610 Acceptable

122-2 Aquatic plants (Tier I), Freshwater algae 45435008 Supplemental

123-2 Aquatic plants (Tier I), Fres hwater algae (Formulation) 45428611 Supplemental

123-2 Vascular Plant, Lemna gibba No study N/A



### Non-target Insects

141-1 Acute Contact, Honey bee Technical 45435007 Acceptable

141-1 Acute Contact, Honeybee Formulation 45428608 Acceptable

Nonguideline Acute Oral, Honeybee (Formulation) 45428608 Supplemental

Non Guideline (OPPTS 850.6200 ) Earthworm 45435009 Supplemental

Non Guideline (OPPTS 850.620 ) Earthworm (Formulation) 45782304 Supplemental

1 Not recommended species; 2 Poor recovery of the test chemical in addition to other guideline deviations; 3 Test chemical was not measured in all test groups; 4 New study using technical ingredient or formulated product is required; 4 New studies submitted since the conditional registration.

## 112 

# Appendix F. The Risk Quotient Method and Levels of Concern

The Risk Quotient Method is the means by which EFED integrates the re sults of exposure and ecotoxicity data. In this method, both acute and chronic risk quotients (RQs) are calculated by dividing exposure estimates by ecoto xicity values or toxicity e ndpoints derived from the studies. Derived RQs are then compared to OPP's levels of concern (LOCs). The LOCs are criteria used by OPP to indicate potential risk to non-target organisms and the need to consider regulatory action. EFED has defined LOCs for acute risk, potential restricted use, and for endangered species. Risk presumptions, along with the corr esponding RQs and LOCs are summarized in the table below.

Risk Presumption RQ LOC

Birds

Acute Risk EEC/LC 50 

### or LD 50 

### /sqft or LD 50 

### /day 0.5

Acute Restricted Use EEC/LC 50 

### or LD 50 

### /sqft or LD 50 

### /day (or LD 50 

### < 50 mg/kg) 0.2

Acute Endangered Species EEC/LC 50 

### or LD 50 

### /sqft or LD 50 

### /day 0.1

Chronic Risk EEC/NOAEC 1

Wild Mammals

Acute Risk EEC/LC 50 

### or LD 50 

### /sqft or LD 50 

### /day 0.5

Acute Restricted Use EEC/LC 50 

### or LD 50 

### /sqft or LD 50 

### /day (or LD 50 

### < 50 mg/kg) 0.2

Acute Endangered Species EEC/LC 50 

### or LD 50 

### /sqft or LD 50 

### /day 0.1

Chronic Risk EEC/NOAEC 1

Aquatic Animals

Acute Risk EEC/LC 50 

### or EC 50 

### 0.5

Acute Restricted Use EEC/LC 50 

### or EC 50 

### 0.1

Acute Endangered Species EEC/LC 50 

### or EC 50 

### 0.05

Chronic Risk EEC/NOAEC 1

Terrestrial and Semi-Aquatic Plants

Acute Risk EEC/EC 25 

### 1

Acute Endangered Species EEC/EC 05 

### or NOAEC 1

Aquatic Plants

Acute Risk EEC/EC 50 

### 1

Acute Endangered Species EEC/EC 05 

### or NOAEC 1

## 113 

# Appendix G. Aquatic RQ Calculations

Surface water estimated environmental concentrations (EECs) resulting from acequinocyl applications to both food and non-food crops we re predicted using th e Tier II PRZM-EXAMS model. Peak EECs were compared to acute toxi city endpoints to derive acute RQs. The 60-day and 21-day EECs are compared to chronic toxicity e ndpoints (NOAEC values) to derive chronic RQs for fish and invert ebrates, respectively.

## 114 

# Appendix H. Endangered Species Summary for Acequinocyl

Organism Scientific Name Status Critical Habitat State Taxa Medium

Kidneyshell, Triangular Ptychobranchus greenii Endangered Yes Alabama Bivalve Freshwater Bat, Gray Myotis grisescens Endangered No Alabama Mammal Subterraneous, Terrestrial Mouse, Perdido Key Beach Peromyscus polionotus trissyllepsis Endangered Yes Alabama Mammal Coastal (neritic)

Mussel, Southern Clubshell Pleurobema decisum Endangered Yes Alabama Bivalve Freshwater

Mouse, Alabama Beach Peromyscus polionotus ammobates Endangered Yes Alabama Mammal Terrestrial, Coastal (neritic) Pearlymussel, Alabama Lamp Lampsilis virescens Endangered No Alabama Bivalve Freshwater

Madtom, Yellowfin Noturus flavipinnis Threatened Yes Alabama Fish Freshwater Cavefish, Alabama Speoplatyrhinus poulsoni Endangered Yes Alabama Fish Freshwater Mussel, Southern Pigtoe Pleurobema georgianum Endangered Yes Alabama Bivalve Freshwater

Turtle, Alabama Redbellied Pseudemys alabamensis Endangered No Alabama Reptile Terrestrial, Freshwater Mussel, Shiny Pigtoe Fusconaia cor Endangered No Alabama Bivalve Freshwater Campeloma, Slender Campeloma decampi Endangered No Alabama Gastropod FreshwaterPearlymussel, Orangefooted Plethobasus cooperianus Endangered No Alabama Bivalve Freshwater

Pearlymussel, Pale Lilliput Toxolasma cylindrellus Endangered No Alabama Bivalve Freshwater

Pearlymussel, Turgidblossom Epioblasma turgidula Endangered No Alabama Bivalve Freshwater

Pearlymussel, White Wartyback Plethobasus cicatricosus Endangered No Alabama Bivalve Freshwater

Mussel, Fine-lined Pocketbook Lampsilis altilis Threatened Yes Alabama Bivalve Freshwater

Pebblesnail, Flat Lepyrium showalteri Endangered No Alabama Gastropod Freshwater Pearlymussel, Cracking Hemistena lata Endangered No Alabama Bivalve Freshwater Mussel, Ovate Clubshell Pleurobema perovatum Endangered Yes Alabama Bivalve Freshwater

Mussel, Dark Pigtoe Pleurobema furvum Endangered Yes Alabama Bivalve Freshwater Mussel, Fine-rayed Pigtoe Fusconaia cuneolus Endangered No Alabama Bivalve Freshwater

Mussel, Flat Pigtoe (=Marshall's Mussel) Pleurobema marshalli Endangered No Alabama Bivalve Freshwater

Chub, Spotfin Erimonax monachus Threatened Yes Alabama Fish Freshwater Mussel, Heavy Pigtoe (=Judge Tait's Mussel) Pleurobema taitianum Endangered No Alabama Bivalve Freshwater

Mussel, Cumberland Combshell Epioblasma brevidens Endangered Yes Alabama Bivalve Freshwater

Mussel, Heelsplitter Inflated Potamilus inflatus Threatened No Alabama Bivalve Freshwater

Mussel, Shiny-rayed Pocketbook Lampsilis subangulata Endangered No Alabama Bivalve Freshwater

Tortoise, Gopher Gopherus polyphemus Threatened No Alabama Reptile Terrestrial

## 115 Organism Scientific Name Status Critical Habitat State Taxa Medium

Mucket, Orangenacre Lampsilis perovalis Threatened Yes Alabama Bivalve Freshwater Combshell, Southern

(=Penitent mussel) Epioblasma penita Endangered No Alabama Bivalve Freshwater

Mussel, Alabama

Moccasinshell Medionidus acutissimus Threatened Yes Alabama Bivalve Freshwater

Mussel, Ring Pink (=Golf Stick Pearly) Obovaria retusa Endangered No Alabama Bivalve Freshwater

Mussel, Acornshell Southern Epioblasma othcaloogensis Endangered Yes Alabama Bivalve Freshwater

Mussel, Rough Pigtoe Pleurobema plenum Endangered No Alabama Bivalve Freshwater

Combshell, Upland Epioblasma metastriata Endangered Yes Alabama Bivalve Freshwater Mucket, Pink (Pearlymussel) Lampsilis abrupta Endangered No Alabama Bivalve Freshwater

Plover, Piping Charadrius melodus Endangered Yes Alabama Bird Terrestrial Mussel, Coosa Moccasinshell Medionidus parvulus Endangered Yes Alabama Bivalve Freshwater

Darter, Boulder Etheostoma wapiti Endangered No Alabama Fish Freshwater Sea turtle, loggerhead Caretta caretta Threatened No Alabama Reptile Saltwater Turtle, Flattened Musk Sternotherus depressus Threatened No Alabama Reptile Freshwater, Terrestrial Shiner, Blue Cyprinella caerulea Threatened No Alabama Fish Freshwater Shiner, Cahaba Notropis cahabae Endangered No Alabama Fish FreshwaterWoodpecker, Redcockaded Picoides borealis Endangered No Alabama Bird Terrestrial

Shiner, Palezone Notropis albizonatus Endangered No Alabama Fish Freshwater Shrimp, Alabama Cave Palaemonias alabamae Endangered No Alabama Crustacean Freshwater Darter, Goldline Percina aurolineata Threatened No Alabama Fish Freshwater

Elimia, Lacy Elimia crenatella Threatened No Alabama Gastropod Freshwater Sculpin, Pygmy Cottus paulus (=pygmaeus) Threatened No Alabama Fish Freshwater

Snail, Lioplax Cylindrical Lioplax cyclostomaformis Endangered No Alabama Gastropod Freshwater Snail, Tulotoma Tulotoma magnifica Endangered No Alabama Gastropod Terrestrial Snake, Eastern Indigo Drymarchon corais couperi Threatened No Alabama Reptile Terrestrial

Eagle, Bald Haliaeetus leucocephalus Threatened No Alabama Bird Terrestrial Darter, Watercress Etheostoma nuchale Endangered No Alabama Fish Freshwater Darter, Vermilion Etheostoma chermocki Endangered No Alabama Fish Freshwater

Darter, Snail Percina tanasi Threatened No Alabama Fish Freshwater Darter, Slackwater Etheostoma boschungi Threatened Yes Alabama Fish Freshwater

Snail, Armored Pyrgulopsis (=Marstonia) pachyta Endangered No Alabama Gastropod Freshwater

Sturgeon, Gulf Acipenser oxyrinchus desotoi Threatened Yes Alabama Fish Saltwater, Freshwater Pearlymussel, Cumberland Monkeyface Quadrula intermedia Endangered No Alabama Bivalve Freshwater

Riversnail, Anthony's Athearnia anthonyi Endangered No Alabama Gastropod Freshwater

Rocksnail, Painted Leptoxis taeniata Threatened No Alabama Gastropod Freshwater

## 116 Organism Scientific Name Status Critical Habitat State Taxa Medium

Rocksnail, Plicate Leptoxis plicata Endangered No Alabama Gastropod Freshwater Bat, Indiana Myotis sodalis Endangered Yes Alabama Mammal Subterraneous, Terrestrial Rocksnail, Round Leptoxis ampla Threatened No Alabama Gastropod Freshwater Stirrupshell Quadrula stapes Endangered No Alabama Bivalve Freshwater Salamander, Flatwoods Ambystoma cingulatum Threatened No Alabama Amphibian Freshwater, Vernal pool, Terrestrial Sturgeon, Alabama Scaphirhynchus suttkusi Endangered No Alabama Fish Freshwater Salamander, Red Hills Phaeognathus hubrichti Threatened No Alabama Amphibian Freshwater, Terrestrial Stork, Wood Mycteria americana Endangered No Alabama Bird Terrestrial Spinedace, Little Colorado Lepidomeda vittata Threatened Yes Arizona Fish Freshwater

Spikedace Meda fulgida Threatened Yes Arizona Fish Freshwater Squawfish, Colorado Ptychocheilus lucius Endangered Yes Arizona Fish Freshwater Pygmy-owl, Cactus Ferruginous Glaucidium brasilianum

cactorum Endangered No Arizona Bird Terrestrial

Pupfish, Desert Cyprinodon macularius Endangered Yes Arizona Fish Freshwater Bobwhite, Masked Colinus virginianus ridgwayi Endangered No Arizona Bird Terrestrial

Pronghorn, Sonoran Antilocapra americana sonoriensis Endangered No Arizona Mammal Terrestrial

Chub, Bonytail Gila elegans Endangered Yes Arizona Fish Freshwater Rail, Yuma Clapper Rallus longirostris yumanensis Endangered No Arizona Bird Terrestrial

Squirrel, Mount Graham Red Tamiasciurus hudsonicus grahamensis Endangered Yes Arizona Mammal Terrestrial

Owl, Mexican Spotted Strix occidentalis lucida Threatened Yes Arizona Bird Terrestrial Chub, Gila Gila intermedia Endangered Yes Arizona Fish Freshwater Sucker, Razorback Xyrauchen texanus Endangered Yes Arizona Fish Freshwater

Ocelot Leopardus (=Felis) pardalis Endangered No Arizona Mammal Terrestrial

Salamander, Sonora Tiger Ambystoma tigrinum stebbinsi Endangered No Arizona Amphibian Vernal pool, Freshwater, Terrestrial Catfish, Yaqui Ictalurus pricei Threatened Yes Arizona Fish Freshwater Chub, Humpback Gila cypha Endangered Yes Arizona Fish Freshwater Bat, Lesser (=Sanborn's) Long-nosed Leptonycteris curasoae yerbabuenae Endangered No Arizona Mammal Subterraneous, Terrestrial Shiner, Beautiful Cyprinella formosa Threatened Yes Arizona Fish Freshwater Rattlesnake, New Mexican Ridge-nosed Crotalus willardi obscurus Threatened Yes Arizona Reptile Terrestrial

Flycatcher, Southwestern Willow Empidonax traillii extimus Endangered Yes Arizona Bird Terrestrial

Ambersnail, Kanab Oxyloma haydeni kanabensis Endangered No Arizona Gastropod Freshwater, Terrestrial Ferret, Black-footed Mustela nigripes Endangered No Arizona Mammal Terrestrial

## 117 Organism Scientific Name Status Critical Habitat State Taxa Medium

Jaguarundi, Sinaloan Herpailurus (=Felis) yagouaroundi tolteca Endangered No Arizona Mammal Terrestrial

Minnow, Loach Tiaroga cobitis Threatened Yes Arizona Fish Freshwater Jaguar Panthera onca Endangered No Arizona Mammal Terrestrial Trout, Gila Oncorhynchus gilae Endangered No Arizona Fish Freshwater Eagle, Bald Haliaeetus leucocephalus Threatened No Arizona Bird Terrestrial Falcon, Northern Aplomado Falco femoralis septentrionalis Endangered No Arizona Bird Terrestrial

Condor, California Gymnogyps californianus Endangered Yes Arizona Bird Terrestrial Wolf, Gray Canis lupus Endangered Yes Arizona Mammal Terrestrial Tortoise, Desert Gopherus agassizii Threatened Yes Arizona Reptile Terrestrial Frog, Chiricahua Leopard Rana chiricahuensis Threatened No Arizona Amphibian Freshwater, Terrestrial Trout, Apache Oncorhynchus apache Threatened No Arizona Fish Freshwater

Woundfin Plagopterus argentissimus Endangered Yes Arizona Fish Freshwater Chub, Yaqui Gila purpurea Endangered Yes Arizona Fish Freshwater Chub, Virgin River Gila seminuda (=robusta) Endangered Yes Arizona Fish Freshwater Vole, Hualapai Mexican Microtus mexicanus hualpaiensis Endangered No Arizona Mammal Terrestrial

Chub, Sonora Gila ditaenia Threatened Yes Arizona Fish Freshwater Topminnow, Gila (Yaqui) Poeciliopsis o ccidentalis Endangered No Arizona Fish Freshwater

Pearlymussel, Fat Pocketbook Potamilus capax Endangered No Arkansas Bivalve Freshwater

Fatmucket, Arkansas Lampsilis powelli Threatened No Arkansas Bivalve Freshwater Bat, Indiana Myotis sodalis Endangered Yes Arkansas Mammal Subterraneous, Terrestrial Shagreen, Magazine Mountain Mesodon magazinensis Threatened No Arkansas Gastropod Terrestrial

Sturgeon, Pallid Scaphirhynchus albus Endangered No Arkansas Fish Freshwater Bat, Ozark Big-eared Corynorhinus (=Plecotus) townsendii ingens Endangered No Arkansas Mammal Terrestrial, Subterraneous Crayfish, Cave (Cambarus zophonastes) Cambarus zophonastes Endangered No Arkansas Crustacean Freshwater

Beetle, American Burying Nicrophorus americanus Endangered No Arkansas Insect Terrestrial

Woodpecker, Redcockaded Picoides borealis Endangered No Arkansas Bird Terrestrial

Rock-pocketbook, Ouachita (=Wheeler's pm) Arkansia wheeleri Endangered No Arkansas Bivalve Freshwater

Mussel, Speckled Pocketbook Lampsilis streckeri Endangered No Arkansas Bivalve Freshwater

Crayfish, Cave (Cambarus aculabrum) Cambarus aculabrum Endangered No Arkansas Crustacean Freshwater

Tern, Interior (population) Least Sterna antillarum Endangered No Arkansas Bird Terrestrial

Cavefish, Ozark Amblyopsis rosae Threatened No Arkansas Fish Freshwater

## 118 Organism Scientific Name Status Critical Habitat State Taxa Medium

Eagle, Bald Haliaeetus leucocephalus Threatened No Arkansas Bird Terrestrial Bat, Gray Myotis grisescens Endangered No Arkansas Mammal Subterraneous, Terrestrial Mussel, Scaleshell Leptodea leptodon Endangered No Arkansas Bivalve Freshwater

Darter, Leopard Percina pantherina Threatened Yes Arkansas Fish Freshwater

Mucket, Pink (Pearlymussel) Lampsilis abrupta Endangered No Arkansas Bivalve Freshwater

Salmon, Chinook (Central Valley Spring Run) Oncorhynchus (=Salmo) tshawytscha Threatened Yes California Fish Brackish, Saltwater, Freshwater

Fly, Delhi Sands Flowerloving Rhaphiomidas terminatus abdominalis Endangered No California Insect Terrestrial

Snake, Giant Garter Thamnophis gigas Threatened No California Reptile Freshwater, Terrestrial Salmon, Chinook (Central Valley Fall Run) Oncorhynchus (=Salmo) tshawytscha Threatened No California Fish Brackish, Freshwater,

Saltwater Salmon, Chinook (California Coastal Run) Oncorhynchus (=Salmo) tshawytscha Threatened Yes California Fish Freshwater, Saltwater, Brackish Snake, San Francisco Garter Thamnophis sirtalis tetrataenia Endangered No California Reptile Freshwater, Terrestrial Flycatcher, Southwestern Willow Empidonax traillii extimus Endangered Yes California Bird Terrestrial

Salamander, Santa Cruz Long-toed Ambystoma macrodactylum croceum Endangered No California Amphibian Freshwater, Vernal pool, Terrestrial Sparrow, San Clemente Sage Amphispiza belli clementeae Threatened No California Bird Terrestrial

Eagle, Bald Haliaeetus leucocephalus Threatened No California Bird Terrestrial Fox, San Joaquin Kit Vulpes macrotis mutica Endangered No California Mammal Terrestrial Fox, San Miguel Island Urocyon littoralis littoralis Endangered Yes California Mammal Terrestrial

Salamander, Desert Slender Batrachoseps aridus Endangered No California Amphibian Freshwater, Terrestrial Fox, Santa Catalina Island Urocyon littoralis catalinae Endangered Yes California Mammal Terrestrial

Salamander, California Tiger Ambystoma californiense Endangered No California Amphibian Terrestrial, Vernal pool Sea turtle, olive ridley Lepidochelys olivacea Threatened No California Reptile Saltwater Shrike, San Clemente Loggerhead Lanius ludovicianus mearnsi Endangered No California Bird Terrestrial

Fairy Shrimp, Riverside Streptocephalus woottoni Endangered Yes California Crustacean Vernal pool Fairy Shrimp, San Diego Branchinecta sandiegonensis Endangered Yes California Crustacean Vernal pool

Goby, Tidewater Eucyclogobius newberryi Endangered Yes California Fish Freshwater Shrimp, California Freshwater Syncaris pacifica Endangered No California Crustacean Freshwater

Fox, Santa Cruz Island Urocyon littoralis

santacruzae Endangered Yes California Mammal Terrestrial

## 119 Organism Scientific Name Status Critical Habitat State Taxa Medium

Fairy Shrimp, Longhorn Branchinecta longiantenna Endangered Yes California Crustacean Vernal pool

Sheep, Sierra Nevada Bighorn Ovis canadensis californiana Endangered No California Mammal Terrestrial

Salmon, Chinook (Sacramento River Winter Run) Oncorhynchus (=Salmo) tshawytscha Endangered No California Fish Saltwater, Freshwater,

Brackish Skipper, Carson

Wandering Pseudocopaeodes eunus obscurus Endangered No California Insect Terrestrial

Fairy Shrimp, Vernal Pool Branchinecta lynchi Threatened Yes California Crustacean Vernal pool

Skipper, Laguna Mountain Pyrgus ruralis lagunae Endangered No California Insect Terrestrial

Smelt, Delta Hypomesus transpacificus Threatened Yes California Fish Freshwater, Brackish Fairy Shrimp, Conservancy Fairy Branchinecta conservatio Endangered Yes California Crustacean Vernal pool

Dace, Ash Meadows Speckled Rhinichthys osculus nevadensis Endangered Yes California Fish Freshwater

Salmon, Coho (Southern OR/Northern CA Coast) Oncorhynchus (=Salmo) kisutch Threatened Yes California Fish Freshwater, Brackish, Saltwater Salmon, Coho (Central California Coast

population) Oncorhynchus (=Salmo) kisutch Endangered No California Fish Saltwater, Brackish, Freshwater

Shrew, Buena Vista Lake Ornate Sorex ornatus relictus Endangered Yes California Mammal Terrestrial

Snail, Morro Shoulderband Helminthoglypta walkeriana Endangered Yes California Gastropod Terrestrial

Sheep, Peninsular Bighorn Ovis canadensis Endangered Yes California Mammal Terrestrial

Condor, California Gymnogyps californianus Endangered Yes California Bird Terrestrial Kangaroo Rat, Stephens' Dipodomys stephensi (incl. D. cascus) Endangered No California Mammal Terrestrial

Butterfly, Myrtle's Silverspot Speyeria zerene myrtleae Endangered No California Insect Terrestrial

Butterfly, Oregon Silverspot Speyeria zerene hippolyta Threatened Yes California Insect Terrestrial

Butterfly, Palos Verdes Blue Glaucopsyche lygdamus palosverdesensis Endangered Yes California Insect Terrestrial

Butterfly, Quino

Checkerspot Euphydryas editha quino (=E. e. wrighti) Endangered Yes California Insect Terrestrial

Butterfly, San Bruno Elfin Callophrys mossii bayensis Endangered No California Insect Terrestrial

Abalone, White Haliotis sorenseni Endangered No California Crustacean Saltwater Butterfly, Smith's Blue Euphilotes enoptes smithi Endangered No California Insect Terrestrial Kangaroo Rat, Tipton Dipodomys nitratoides nitratoides Endangered No California Mammal Terrestrial

Lizard, Blunt-nosed Leopard Gambelia silus Endangered No California Reptile Terrestrial

## 120 Organism Scientific Name Status Critical Habitat State Taxa Medium

Gnatcatcher, Coastal California Polioptila californica californica Threatened Yes California Bird Terrestrial

Lizard, Island Night Xantusia riversiana Threatened No California Reptile Terrestrial Butterfly, Mission Blue Icaricia icarioides missionensis Endangered No California Insect Terrestrial

Owl, Northern Spotted Strix occidentalis caurina Threatened Yes California Bird Terrestrial Moth, Kern Primrose Sphinx Euproserpinus euterpe Threatened No California Insect Terrestrial

Chub, Bonytail Gila elegans Endangered Yes California Fish Freshwater Mountain Beaver, Point Arena Aplodontia rufa nigra Endangered No California Mammal Freshwater, Terrestrial Rail, Light-footed Clapper Rallus longirostris levipes Endangered No California Bird Terrestrial

Mouse, Pacific Pocket Perognathus longimembris pacificus Endangered No California Mammal Terrestrial

Mouse, Salt Marsh Harvest Reithrodontomys raviventris Endangered No California Mammal Terrestrial

Murrelet, Marbled Brachyramphus

marmoratus marmoratus Threatened Yes California Bird Freshwater, Terrestrial, Saltwater Chub, Hutton Tui Gila bicolor ssp. Threatened No California Fish Freshwater Chub, Mohave Tui Gila bicolor mohavensis Endangered No California Fish Freshwater Lizard, Coachella Valley Fringe-toed Uma inornata Threatened Yes California Reptile Terrestrial

Crayfish, Shasta Pacifastacus fortis Endangered No California Crustacean FreshwaterFrog, California Redlegged Rana aurora draytonii Threatened Yes California Amphibian Terrestrial, Freshwater Beetle, Delta Green Ground Elaphrus viridis Threatened Yes California Insect Vernal pool, Terrestrial Beetle, Mount Hermon June Polyphylla barbata Endangered No California Insect Subterraneous, Terrestrial Rail, Yuma Clapper Rallus longirostris yumanensis Endangered No California Bird Terrestrial

Frog, Mountain Yellowlegged Rana muscosa Endangered No California Amphibian Terrestrial, Freshwater Rail, California Clapper Rallus longirostris obsoletus Endangered No California Bird Terrestrial

Chub, Owens Tui Gila bicolor snyderi Endangered Yes California Fish Freshwater Rabbit, Riparian Brush Sylvilagus bachmani riparius Endangered No California Mammal Terrestrial

Grasshopper, Zayante Band-winged Trimerotropis infantilis Endangered Yes California Insect Terrestrial

Pupfish, Owens Cyprinodon radiosus Endangered No California Fish Freshwater Kangaroo Rat, San Bernardino Merriam's Dipodomys merriami

parvus Endangered Yes California Mammal Terrestrial

Pupfish, Desert Cyprinodon macularius Endangered Yes California Fish Freshwater Kangaroo Rat, Morro Bay Dipodomys heermanni morroensis Endangered Yes California Mammal Terrestrial

## 121 Organism Scientific Name Status Critical Habitat State Taxa Medium

Butterfly, Bay Checkerspot (Wright's euphydryas) Euphydryas editha bayensis Threatened Yes California Insect Terrestrial

Butterfly, Behren's Silverspot Speyeria zerene behrensii Endangered No California Insect Terrestrial

Butterfly, Callippe Silverspot Speyeria callippe callippe Endangered No California Insect Terrestrial

Butterfly, El Segundo Blue Euphilotes battoides allyni Endangered No California Insect Terrestrial

Plover, Western Snowy Charadrius alexandrinus nivosus Threatened Yes California Bird Terrestrial

Pelican, Brown Pelecanus occidentalis Endangered No California Bird Terrestrial Kangaroo Rat, Fresno Dipodomys nitratoides exilis Endangered Yes California Mammal Terrestrial

Kangaroo Rat, Giant Dipodomys ingens Endangered No California Mammal Terrestrial

Butterfly, Lange's Metalmark Apodemia mormo langei Endangered No California Insect Terrestrial

Butterfly, Lotis Blue Lycaeides argyrognomon lotis Endangered No California Insect Terrestrial

Fox, Santa Rosa Island Urocyon littoralis

santarosae Endangered Yes California Mammal Terrestrial

Beetle, Valley Elderberry Longhorn Desmocerus californicus dimorphus Threatened Yes California Insect Terrestrial

Tadpole Shrimp, Vernal Pool Lepidurus packardi Endangered Yes California Crustacean Vernal pool

Squawfish, Colorado Ptychocheilus lucius Endangered Yes California Fish Freshwater Steelhead, (Northern California population) Oncorhynchus (=Salmo) mykiss Threatened No California Fish Saltwater, Brackish, Freshwater Trout, Little Kern Golden Oncorhynchus aguabonita whitei Threatened Yes California Fish Freshwater

Trout, Paiute Cutthroat Oncorhynchus clarki seleniris Threatened No California Fish Freshwater

Beetle, Ohlone Tiger Cicindela ohlone Endangered No California Insect Terrestrial Steelhead, (Central

California Coast

population) Oncorhynchus (=Salmo) mykiss Threatened Yes California Fish Freshwater, Saltwater, Brackish

Sucker, Santa Ana Catostomus santaanae Threatened Yes California Fish Freshwater Sucker, Razorback Xyrauchen texanus Endangered Yes California Fish Freshwater Vireo, Least Bell's Vireo bellii pusillus Endangered Yes California Bird Terrestrial Steelhead, (California Central Valley

population) Oncorhynchus (=Salmo) mykiss Threatened Yes California Fish Brackish, Freshwater,

Saltwater Vole, Amargosa Microtus californicus scirpensis Endangered Yes California Mammal Terrestrial

Tern, California Least Sterna antillarum browni Endangered No California Bird Terrestrial Towhee, Inyo Brown Pipilo crissalis eremophilus Threatened Yes California Bird Terrestrial

Tortoise, Desert Gopherus agassizii Threatened Yes California Reptile Terrestrial

## 122 Organism Scientific Name Status Critical Habitat State Taxa Medium

Whipsnake (=Striped

Racer), Alameda Masticophis lateralis euryxanthus Threatened Yes California Reptile Terrestrial

Sturgeon, green Acipenser medirostris Threatened No California Fish Toad, Arroyo Southwestern Bufo californicus (=microscaphus) Endangered Yes California Amphibian Freshwater, Terrestrial Stickleback, Unarmored Threespine Gasterosteus aculeatus williamsoni Endangered No California Fish Freshwater

Sucker, Shortnose Chasmistes brevirostris Endangered No California Fish Freshwater Sucker, Lost River Deltistes luxatus Endangered No California Fish Freshwater Steelhead, (Southern California population) Oncorhynchus (=Salmo) mykiss Endangered Yes California Fish Brackish, Saltwater, Freshwater Sucker, Modoc Catostomus microps Endangered Yes California Fish Freshwater Trout, Lahontan Cutthroat Oncorhynchus clarki henshawi Threatened No California Fish Freshwater

Woodrat, Riparian Neotoma fuscipes riparia Endangered No California Mammal Terrestrial Steelhead, (South-Central

California population) Oncorhynchus (=Salmo) mykiss Threatened Yes California Fish Freshwater, Saltwater, Brackish Butterfly, Uncompahgre Fritillary Boloria acrocnema Endangered No Colorado Insect Terrestrial

Mouse, Preble's Meadow Jumping Zapus hudsonius preblei Threatened Yes Colorado Mammal Terrestrial

Owl, Mexican Spotted Strix occidentalis lucida Threatened Yes Colorado Bird Terrestrial Chub, Bonytail Gila elegans Endangered Yes Colorado Fish Freshwater Trout, Greenback Cutthroat Oncorhynchus clarki stomias Threatened No Colorado Fish Freshwater

Skipper, Pawnee Montane Hesperia leonardus

montana Threatened No Colorado Insect Terrestrial

Trout, Bull Salvelinus confluentus Threatened No Colorado Fish Freshwater Ferret, Black-footed Mustela nigripes Endangered No Colorado Mammal Terrestrial Squawfish, Colorado Ptychocheilus lucius Endangered Yes Colorado Fish Freshwater

Chub, Humpback Gila cypha Endangered Yes Colorado Fish Freshwater Eagle, Bald Haliaeetus leucocephalus Threatened No Colorado Bird Terrestrial Sucker, Razorback Xyrauchen texanus Endangered Yes Colorado Fish Freshwater Crane, Whooping Grus americana Endangered Yes Colorado Bird Terrestrial, Freshwater Sturgeon, Shortnose Acipenser brevirostrum Endangered No Connecticut Fish Saltwater, Freshwater Bat, Indiana Myotis sodalis Endangered Yes Connecticut Mammal Subterraneous, Terrestrial Eagle, Bald Haliaeetus leucocephalus Threatened No Connecticut Bird Terrestrial Mussel, Dwarf Wedge Alasmidonta heterodon Endangered No Connecticut Bivalve Freshwater Turtle, Bog (Northern population) Clemmys muhlenbergii Threatened No Connecticut Reptile Terrestrial, Freshwater Plover, Piping Charadrius melodus Endangered Yes Connecticut Bird Terrestrial Beetle, Puritan Tiger Cicindela puritana Threatened No Connecticut Insect Terrestrial, Coastal (neritic) Tern, Roseate Sterna dougallii dougallii Endangered No Connecticut Bird Terrestrial

## 123 Organism Scientific Name Status Critical Habitat State Taxa Medium

Squirrel, Delmarva

Peninsula Fox Sciurus niger cinereus Endangered No Delaware Mammal Terrestrial

Eagle, Bald Haliaeetus leucocephalus Threatened No Delaware Bird Terrestrial Turtle, Bog (Northern population) Clemmys muhlenbergii Threatened No Delaware Reptile Terrestrial, Freshwater Sturgeon, Shortnose Acipenser brevirostrum Endangered No Delaware Fish Saltwater, Freshwater Plover, Piping Charadrius melodus Endangered Yes Delaware Bird Terrestrial Vole, Florida Salt Marsh Microtus pennsylvanicus dukecampbelli Endangered No Florida Mammal Terrestrial, Brackish Sea turtle, hawksbill Eretmochelys imbricata Endangered Yes Florida Reptile Saltwater Mussel, Gulf Moccasinshell Medionidus penicillatus Endangered No Florida Bivalve Freshwater

Stork, Wood Mycteria americana Endangered No Florida Bird Terrestrial Mouse, Key Largo Cotton Peromyscus gossypinus allapaticola Endangered No Florida Mammal Terrestrial

Sea turtle, leatherback Dermochelys coriacea Endangered Yes Florida Reptile Saltwater Mouse, Choctawhatchee Beach Peromyscus polionotus allophrys Endangered Yes Florida Mammal Coastal (neritic), Terrestrial Mouse, Anastasia Island Beach Peromyscus polionotus phasma Endangered No Florida Mammal Terrestrial, Coastal (neritic) Bankclimber, Purple Elliptoideus sloatianus Threatened No Florida Bivalve Freshwater Sea turtle, Kemp's ridley Lepidochelys kempii Endangered No Florida Reptile Saltwater

Kite, Everglade Snail Rostrhamus sociabilis plumbeus Endangered Yes Florida Bird Terrestrial

Sea turtle, loggerhead Caretta caretta Threatened No Florida Reptile Saltwater Mussel, Ochlockonee Moccasinshell Medionidus simpsonianus Endangered No Florida Bivalve Freshwater

Bat, Gray Myotis grisescens Endangered No Florida Mammal Subterraneous, Terrestrial Mouse, Perdido Key Beach Peromyscus polionotus trissyllepsis Endangered Yes Florida Mammal Coastal (neritic)

Skink, Sand Neoseps reynoldsi Threatened No Florida Reptile Terrestrial Skink, Blue-tailed Mole Eumeces egregius lividus Threatened No Florida Reptile Terrestrial Shrimp, Squirrel Chimney Cave Palaemonetes cummingi Threatened No Florida Crustacean Freshwater, Subterraneous Crocodile, American Crocodylus acutus Threatened Yes Florida Reptile Terrestrial, Freshwater Sea turtle, green Chelonia mydas Endangered No Florida Reptile Saltwater

Bat, Indiana Myotis sodalis Endangered Yes Florida Mammal Subterraneous, Terrestrial Sawfish, Smalltooth Pristis pectinata Endangered No Florida Fish Saltwater, Freshwater Mouse, Southeastern Beach Peromyscus polionotus niveiventris Threatened No Florida Mammal Coastal (neritic), Terrestrial Scrub-Jay, Florida Aphelocoma coerulescens Threatened No Florida Bird Terrestrial Slabshell, Chipola Elliptio chipolaensis Threatened No Florida Bivalve Freshwater

## 124 Organism Scientific Name Status Critical Habitat State Taxa Medium

Sturgeon, Shortnose Acipenser brevirostrum Endangered No Florida Fish Saltwater, Freshwater Rice Rat (=Silver Rice Rat) Oryzomys palustris

natator Endangered Yes Florida Mammal Terrestrial

Panther, Florida Puma (=Felis) concolor coryi Endangered No Florida Mammal Terrestrial

Caracara, Audubon's Crested Polyborus plancus audubonii Threatened No Florida Bird Terrestrial

Butterfly, Schaus Swallowtail Heraclides aristodemus

ponceanus Endangered No Florida Insect Terrestrial

Threeridge, Fat (Mussel) Amblema neislerii Endangered No Florida Bivalve Freshwater

Rabbit, Lower Keys Marsh Sylvilagus palustris hefneri Endangered No Florida Mammal Terrestrial

Snake, Atlantic Salt Marsh Nerodia clarkii taeniata Threatened No Florida Reptile Saltwater, Terrestrial, Brackish Mussel, Shiny-rayed Pocketbook Lampsilis subangulata Endangered No Florida Bivalve Freshwater

Snake, Eastern Indigo Drymarchon corais couperi Threatened No Florida Reptile Terrestrial

Snail, Stock Island Tree Orthalicus reses (not incl. nesodryas) Threatened No Florida Gastropod Terrestrial

Sparrow, Cape Sable Seaside Ammodramus maritimus mirabilis Endangered Yes Florida Bird Terrestrial

Sparrow, Florida

Grasshopper Ammodramus savannarum floridanus Endangered No Florida Bird Terrestrial

Eagle, Bald Haliaeetus leucocephalus Threatened No Florida Bird Terrestrial Deer, Key Odocoileus virginianus clavium Endangered No Florida Mammal Terrestrial

Woodrat, Key Largo Neotoma floridana smalli Endangered No Florida Mammal Terrestrial

Darter, Okaloosa Etheostoma okaloosae Endangered No Florida Fish Freshwater

Plover, Piping Charadrius melodus Endangered Yes Florida Bird TerrestrialWoodpecker, Redcockaded Picoides borealis Endangered No Florida Bird Terrestrial

Tern, Roseate Sterna dougallii dougallii Endangered No Florida Bird Terrestrial Mussel, Oval Pigtoe Pleurobema pyriforme Endangered No Florida Bivalve Freshwater Salamander, Flatwoods Ambystoma cingulatum Threatened No Florida Amphibian Freshwater, Vernal pool, Terrestrial Sturgeon, Gulf Acipenser oxyrinchus desotoi Threatened Yes Florida Fish Saltwater, Freshwater Bat, Virginia Big-eared Corynorhinus (=Plecotus) townsendii virginianus Endangered Yes Georgia Mammal Terrestrial, Subterraneous Shiner, Blue Cyprinella caerulea Threatened No Georgia Fish Freshwater Darter, Amber Percina antesella Endangered Yes Georgia Fish Freshwater Mussel, Ovate Clubshell Pleurobema perovatum Endangered Yes Georgia Bivalve Freshwater

Sturgeon, Shortnose Acipenser brevirostrum Endangered No Georgia Fish Saltwater, Freshwater Logperch, Conasauga Percina jenkinsi Endangered Yes Georgia Fish Freshwater

## 125 Organism Scientific Name Status Critical Habitat State Taxa Medium

Madtom, Yellowfin Noturus flavipinnis Threatened Yes Georgia Fish Freshwater Combshell, Upland Epioblasma metastriata Endangered Yes Georgia Bivalve Freshwater Eagle, Bald Haliaeetus leucocephalus Threatened No Georgia Bird Terrestrial Warbler (=Wood), Kirtland's Dendroica kirtlandii Endangered No Georgia Bird Terrestrial

Mucket, Pink (Pearlymussel) Lampsilis abrupta Endangered No Georgia Bivalve Freshwater

Snake, Eastern Indigo Drymarchon corais couperi Threatened No Georgia Reptile Terrestrial

Darter, Cherokee Etheostoma scotti Threatened No Georgia Fish Freshwater Sea turtle, loggerhead Caretta caretta Threatened No Georgia Reptile Saltwater Darter, Snail Percina tanasi Threatened No Georgia Fish Freshwater Mussel, Oval Pigtoe Pleurobema pyriforme Endangered No Georgia Bivalve Freshwater

Darter, Etowah Etheostoma etowahae Endangered No Georgia Fish Freshwater Bankclimber, Purple Elliptoideus sloatianus Threatened No Georgia Bivalve Freshwater Stork, Wood Mycteria americana Endangered No Georgia Bird Terrestrial Darter, Goldline Percina aurolineata Threatened No Georgia Fish Freshwater Plover, Piping Charadrius melodus Endangered Yes Georgia Bird Terrestrial Threeridge, Fat (Mussel) Amblema neislerii Endangered No Georgia Bivalve Freshwater

Mussel, Acornshell Southern Epioblasma othcaloogensis Endangered Yes Georgia Bivalve Freshwater

Mussel, Shiny-rayed Pocketbook Lampsilis subangulata Endangered No Georgia Bivalve Freshwater

Mussel, Coosa Moccasinshell Medionidus parvulus Endangered Yes Georgia Bivalve Freshwater

Mussel, Southern Clubshell Pleurobema decisum Endangered Yes Georgia Bivalve Freshwater

Mussel, Southern Pigtoe Pleurobema georgianum Endangered Yes Georgia Bivalve Freshwater Mussel, Alabama

Moccasinshell Medionidus acutissimus Threatened Yes Georgia Bivalve Freshwater

Sturgeon, Gulf Acipenser oxyrinchus desotoi Threatened Yes Georgia Fish Saltwater, FreshwaterWoodpecker, Redcockaded Picoides borealis Endangered No Georgia Bird Terrestrial

Chub, Spotfin Erimonax monachus Threatened Yes Georgia Fish Freshwater

Bat, Gray Myotis grisescens Endangered No Georgia Mammal Subterraneous, Terrestrial Beetle, American Burying Nicrophorus americanus Endangered No Georgia Insect Terrestrial

Kidneyshell, Triangular Ptychobranchus greenii Endangered Yes Georgia Bivalve Freshwater

Mussel, Fine-lined Pocketbook Lampsilis altilis Threatened Yes Georgia Bivalve Freshwater

Mussel, Gulf Moccasinshell Medionidus penicillatus Endangered No Georgia Bivalve Freshwater

Salamander, Flatwoods Ambystoma cingulatum Threatened No Georgia Amphibian Freshwater, Vernal pool, Terrestrial

Fanshell Cyprogenia stegaria Endangered No Georgia Bivalve Freshwater

## 126 Organism Scientific Name Status Critical Habitat State Taxa Medium

Bat, Indiana Myotis sodalis Endangered Yes Georgia Mammal Subterraneous, Terrestrial Stilt, Hawaiian (=Ae'o) Himantopus mexicanus knudseni Endangered No Hawaii Bird Terrestrial

Bat, Hawaiian Hoary Lasiurus cinereus semotus Endangered No Hawaii Mammal Terrestrial, Subterraneous

Nuku Pu'u Hemignathus lucidus Endangered No Hawaii Bird Terrestrial 'O'o, Kauai (='A'a) Moho braccatus Endangered No Hawaii Bird Terrestrial 'O'u (Honeycreeper) Psittirostra psittacea Endangered No Hawaii Bird Terrestrial

Sea turtle, green Chelonia mydas Endangered No Hawaii Reptile Saltwater Thrush, Large Kauai Myadestes myadestinus Endangered No Hawaii Bird TerrestrialPetrel, Hawaiian Darkrumped Pterodroma phaeopygia sandwichensis Endangered No Hawaii Bird Terrestrial

Po'ouli Melamprosops phaeosoma Endangered No Hawaii Bird Terrestrial Thrush, Molokai (Oloma'o) Myadestes lanaiensis rutha Endangered No Hawaii Bird Terrestrial

Thrush, Small Kauai (Puaiohi) Myadestes palmeri Endangered No Hawaii Bird Terrestrial

Sea turtle, hawksbill Eretmochelys imbricata Endangered Yes Hawaii Reptile Saltwater

Parrotbill, Maui Pseudonestor xanthophrys Endangered No Hawaii Bird Terrestrial

Palila Loxioides bailleui Endangered Yes Hawaii Bird Terrestrial Snail, O'ahu Tree (Achatinella dimorpha) Achatinella dimorpha Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella bulimoides) Achatinella bulimoides Endangered No Hawaii Gastropod Terrestrial

Millerbird, Nihoa Acrocephalus familiaris kingi Endangered No Hawaii Bird Terrestrial

Snail, O'ahu Tree (Achatinella leucorraphe) Achatinella leucorraphe Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella lehuiensis) Achatinella lehuiensis Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella juncea) Achatinella juncea Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella juddii) Achatinella juddii Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella fuscobasis) Achatinella fuscobasis Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella fulgens) Achatinella fulgens Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella lorata) Achatinella lorata Endangered No Hawaii Gastropod Terrestrial

Spider, Kauai Cave Wolf Adelocosa anops Endangered Yes Hawaii Arachnid Terrestrial, Subterraneous Snail, O'ahu Tree (Achatinella mustelina) Achatinella mustelina Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella lila) Achatinella lila Endangered No Hawaii Gastropod Terrestrial

## 127 Organism Scientific Name Status Critical Habitat State Taxa Medium

Snail, O'ahu Tree (Achatinella decipiens) Achatinella decipiens Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella curta) Achatinella curta Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree

(Achatinella concavospira) Achatinella concavospira Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella cestus) Achatinella cestus Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella casta) Achatinella casta Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella caesia) Achatinella caesia Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella byronii) Achatinella byronii Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella elegans) Achatinella elegans Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella swiftii) Achatinella swiftii Endangered No Hawaii Gastropod Terrestrial

'Akia Loa, Kauai (Hemignathus procerus) Hemignathus procerus Endangered No Hawaii Bird Terrestrial

Duck, Laysan Anas laysanensis Endangered No Hawaii Bird Terrestrial, Freshwater Duck, Hawaiian (Koloa) Anas wyvilliana Endangered No Hawaii Bird Freshwater, Terrestrial Elepaio, Oahu Chasiempis sandwichensis ibidis Endangered Yes Hawaii Bird Terrestrial

'Akia Pola'au (Hemignathus munroi) Hemignathus munroi Endangered No Hawaii Bird Terrestrial

Albatross, Short-tailed Phoebastria (=Diomedea) albatrus Endangered No Hawaii Bird Terrestrial, Saltwater Snail, O'ahu Tree (Achatinella valida) Achatinella valida Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella turgida) Achatinella turgida Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella livida) Achatinella livida Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella taeniolata) Achatinella taeniolata Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella decora) Achatinella decora Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella stewartii) Achatinella stewartii Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella spaldingi) Achatinella spaldingi Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella sowerbyana) Achatinella sowerbyana Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella rosea) Achatinella rosea Endangered No Hawaii Gastropod Terrestrial

## 128 Organism Scientific Name Status Critical Habitat State Taxa Medium

Snail, O'ahu Tree (Achatinella pupukanioe) Achatinella pupukanioe Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella pulcherrima) Achatinella pulcherrima Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella phaeozona) Achatinella phaeozona Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella papyracea) Achatinella papyracea Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella thaanumi) Achatinella thaanumi Endangered No Hawaii Gastropod Terrestrial

Hawk, Hawaiian (Io) Buteo solitarius Endangered No Hawaii Bird Terrestrial

Moth, Blackburn's Sphinx Manduca blackburni Endangered Yes Hawaii Insect Terrestrial

Moorhen, Hawaiian Common Gallinula chloropus sandvicensis Endangered No Hawaii Bird Terrestrial

Coot, Hawaiian (=Alae keo keo) Fulica americana alai Endangered No Hawaii Bird Terrestrial

Shearwater, Newell's Townsend's Puffinus auricularis newelli Threatened No Hawaii Bird Terrestrial, Saltwater 'Akepa, Maui Loxops coccineus ochraceus Endangered No Hawaii Bird Terrestrial

Honeycreeper, Crested ('Akohekohe) Palmeria dolei Endangered No Hawaii Bird Terrestrial

Snail, O'ahu Tree (Achatinella buddii) Achatinella buddii Endangered No Hawaii Gastropod Terrestrial

Creeper, Hawaii Oreomystis mana Endangered No Hawaii Bird Terrestrial Goose, Hawaiian (Nene) Branta (=Nesochen) sandvicensis Endangered No Hawaii Bird Terrestrial, Freshwater Creeper, Molokai

(Kakawahie) Paroreomyza flammea Endangered No Hawaii Bird Terrestrial

Creeper, Oahu (Alauwahio) Paroreomyza maculata Endangered No Hawaii Bird Terrestrial

Crow, Hawaiian ('Alala) Corvus hawaiiensis Endangered No Hawaii Bird Terrestrial Amphipod, Kauai Cave Spelaeorchestia koloana Endangered Yes Hawaii Crustacean Freshwater, Subterraneous Finch, Nihoa Telespyza ultima Endangered No Hawaii Bird Terrestrial Snail, O'ahu Tree (Achatinella abbreviata) Achatinella abbreviata Endangered No Hawaii Gastropod Terrestrial

Finch, Laysan Telespyza cantans Endangered No Hawaii Bird Terrestrial Snail, Newcomb's Erinna newcombi Threatened Yes Hawaii Gastropod Freshwater

'Akepa, Hawaii Loxops coccineus coccineus Endangered No Hawaii Bird Terrestrial

Snail, O'ahu Tree (Achatinella apexfulva) Achatinella apexfulva Endangered No Hawaii Gastropod Terrestrial

Snail, O'ahu Tree (Achatinella bellula) Achatinella bellula Endangered No Hawaii Gastropod Terrestrial

Sturgeon, White Acipenser transmontanus Endangered Yes Idaho Fish Saltwater, Freshwater

## 129 Organism Scientific Name Status Critical Habitat State Taxa Medium

Snail, Utah Valvata Valvata utahensis Endangered No Idaho Gastropod Terrestrial Trout, Bull (Columbia

River population) Salvelinus confluentus Threatened Yes Idaho Fish Freshwater

Crane, Whooping Grus americana Endangered Yes Idaho Bird Terrestrial, Freshwater Salmon, Chinook (Snake River Fall Run) Oncorhynchus (=Salmo) tshawytscha Threatened No Idaho Fish Freshwater, Saltwater, Brackish Caribou, Woodland Rangifer tarandus caribou Endangered No Idaho Mammal Terrestrial Limpet, Banbury Springs Lanx sp. Endangered No Idaho Gastropod Freshwater Snail, Bliss Rapids Taylorconcha serpenticola Threatened No Idaho Gastropod Freshwater Springsnail, Idaho Fontelicella idahoensis Endangered No Idaho Gastropod Freshwater Steelhead, (Snake River Basin population) Oncorhynchus (=Salmo) mykiss Threatened Yes Idaho Fish Freshwater, Brackish, Saltwater Salmon, Chinook (Snake

River spring/summer) Oncorhynchus (=Salmo) tshawytscha Threatened Yes Idaho Fish Brackish, Saltwater, Freshwater Trout, Bull Salvelinus confluentus Threatened No Idaho Fish Freshwater Snail, Snake River Physa Physa natricina Endangered No Idaho Gastropod Terrestrial Springsnail, Bruneau Hot Pyrgulopsis bruneauensis Endangered No Idaho Gastropod Freshwater Eagle, Bald Haliaeetus leucocephalus Threatened No Idaho Bird Terrestrial Wolf, Gray Canis lupus Endangered Yes Idaho Mammal Terrestrial Squirrel, Northern Idaho Ground Spermophilus brunneus brunneus Threatened No Idaho Mammal Terrestrial

Salmon, Sockeye (Snake River population) Oncorhynchus (=Salmo) nerka Endangered No Idaho Fish Brackish, Saltwater, Freshwater Bear, Grizzly Ursus arctos horribilis Threatened No Idaho Mammal Terrestrial Trout, Bull (Klamath

River population) Salvelinus confluentus Threatened Yes Idaho Fish Freshwater

Eagle, Bald Haliaeetus leucocephalus Threatened No Illinois Bird Terrestrial Butterfly, Karner Blue Lycaeides melissa samuelis Endangered No Illinois Insect Terrestrial

Pearlymussel, White Wartyback Plethobasus cicatricosus Endangered No Illinois Bivalve Freshwater

Pearlymussel, Fat Pocketbook Potamilus capax Endangered No Illinois Bivalve Freshwater

Pearlymussel, Higgins' Eye Lampsilis higginsii Endangered No Illinois Bivalve Freshwater

Pearlymussel, Orangefooted Plethobasus cooperianus Endangered No Illinois Bivalve Freshwater

Amphipod, Illinois Cave Gammarus acherondytes Endangered No Illinois Crustacean Subterraneous, Freshwater Plover, Piping Charadrius melodus Endangered Yes Illinois Bird Terrestrial Mucket, Pink (Pearlymussel) Lampsilis abrupta Endangered No Illinois Bivalve Freshwater

Tern, Interior (population) Least Sterna antillarum Endangered No Illinois Bird Terrestrial

Bat, Gray Myotis grisescens Endangered No Illinois Mammal Subterraneous, Terrestrial Sturgeon, Pallid Scaphirhynchus albus Endangered No Illinois Fish Freshwater

## 130 Organism Scientific Name Status Critical Habitat State Taxa Medium

Fanshell Cyprogenia stegaria Endangered No Illinois Bivalve Freshwater Bat, Indiana Myotis sodalis Endangered Yes Illinois Mammal Subterraneous, Terrestrial Mussel, Clubshell Pleurobema clava Endangered No Illinois Bivalve Freshwater Snail, Iowa Pleistocene Discus macclintocki Endangered No Illinois Gastropod Terrestrial

Dragonfly, Hine's Emerald Somatochlora hineana Endangered No Illinois Insect Freshwater, Terrestrial Pearlymussel, White Cat's Paw Epioblasma obliquata perobliqua Endangered No Indiana Bivalve Freshwater

Butterfly, Karner Blue Lycaeides melissa samuelis Endangered No Indiana Insect Terrestrial

Plover, Piping Charadrius melodus Endangered Yes Indiana Bird Terrestrial Pearlymussel, White Wartyback Plethobasus cicatricosus Endangered No Indiana Bivalve Freshwater

Pearlymussel, Tubercledblossom Epioblasma torulosa torulosa Endangered No Indiana Bivalve Freshwater

Tern, Interior (population) Least Sterna antillarum Endangered No Indiana Bird Terrestrial

Pearlymussel, Fat Pocketbook Potamilus capax Endangered No Indiana Bivalve Freshwater

Mucket, Pink (Pearlymussel) Lampsilis abrupta Endangered No Indiana Bivalve Freshwater

Mussel, Clubshell Pleurobema clava Endangered No Indiana Bivalve Freshwater Bat, Indiana Myotis sodalis Endangered Yes Indiana Mammal Subterraneous, Terrestrial Mussel, Ring Pink (=Golf Stick Pearly) Obovaria retusa Endangered No Indiana Bivalve Freshwater

Mussel, Rough Pigtoe Pleurobema plenum Endangered No Indiana Bivalve Freshwater Bat, Gray Myotis grisescens Endangered No Indiana Mammal Subterraneous, Terrestrial Riffleshell, Northern Epioblasma torulosa rangiana Endangered No Indiana Bivalve Freshwater

Butterfly, Mitchell's Satyr Neonympha mitchellii mitchellii Endangered No Indiana Insect Terrestrial

Fanshell Cyprogenia stegaria Endangered No Indiana Bivalve Freshwater Snake, Northern Copperbelly Water Nerodia erythrogaster neglecta Threatened No Indiana Reptile Freshwater, Terrestrial Eagle, Bald Haliaeetus leucocephalus Threatened No Indiana Bird TerrestrialPearlymussel, Orangefooted Plethobasus cooperianus Endangered No Indiana Bivalve Freshwater

Pearlymussel, Fat Pocketbook Potamilus capax Endangered No Iowa Bivalve Freshwater

Eagle, Bald Haliaeetus leucocephalus Threatened No Iowa Bird Terrestrial Pearlymussel, Higgins' Eye Lampsilis higginsii Endangered No Iowa Bivalve Freshwater

Plover, Piping Charadrius melodus Endangered Yes Iowa Bird Terrestrial Tern, Interior (population) Least Sterna antillarum Endangered No Iowa Bird Terrestrial

## 131 Organism Scientific Name Status Critical Habitat State Taxa Medium

Bat, Indiana Myotis sodalis Endangered Yes Iowa Mammal Subterraneous, Terrestrial Snail, Iowa Pleistocene Discus macclintocki Endangered No Iowa Gastropod Terrestrial Sturgeon, Pallid Scaphirhynchus albus Endangered No Iowa Fish Freshwater Shiner, Topeka Notropis topeka (=tristis) Endangered Yes Iowa Fish Freshwater Madtom, Neosho Noturus placidus Threatened No Kansas Fish Freshwater

Tern, Interior (population) Least Sterna antillarum Endangered No Kansas Bird Terrestrial

Beetle, American Burying Nicrophorus americanus Endangered No Kansas Insect Terrestrial

Shiner, Arkansas River Notropis girardi Threatened Yes Kansas Fish Freshwater

Crane, Whooping Grus americana Endangered Yes Kansas Bird Terrestrial, Freshwater Sturgeon, Pallid Scaphirhynchus albus Endangered No Kansas Fish Freshwater Ferret, Black-footed Mustela nigripes Endangered No Kansas Mammal Terrestrial Bat, Gray Myotis grisescens Endangered No Kansas Mammal Subterraneous, Terrestrial Shiner, Topeka Notropis topeka (=tristis) Endangered Yes Kansas Fish Freshwater

Eagle, Bald Haliaeetus leucocephalus Threatened No Kansas Bird Terrestrial Plover, Piping Charadrius melodus Endangered Yes Kansas Bird Terrestrial

Bat, Gray Myotis grisescens Endangered No Kentucky Mammal Subterraneous, Terrestrial Pearlymussel, Appalachian Monkeyface Quadrula sparsa Endangered No Kentucky Bivalve Freshwater

Pearlymussel, Purple Cat's Paw Epioblasma obliquata obliquata Endangered No Kentucky Bivalve Freshwater

Beetle, American Burying Nicrophorus americanus Endangered No Kentucky Insect Terrestrial

Sturgeon, Pallid Scaphirhynchus albus Endangered No Kentucky Fish Freshwater Warbler (=Wood), Kirtland's Dendroica kirtlandii Endangered No Kentucky Bird Terrestrial

Riffleshell, Tan Epioblasma florentina walkeri (=E. walkeri) Endangered No Kentucky Bivalve Freshwater

Mussel, Winged Mapleleaf Quadrula fragosa Endangered No Kentucky Bivalve Freshwater

Warbler, Bachman's Vermivora bachmanii Endangered No Kentucky Bird Terrestrial Woodpecker, Ivory-billed Campephilus principalis Endangered No Kentucky Bird Terrestrial Pearlymussel, Little-wing Pegias fabula Endangered No Kentucky Bivalve Freshwater

Mussel, Cumberland Elktoe Alasmidonta atropurpurea Endangered Yes Kentucky Bivalve Freshwater

Mussel, Cumberland Combshell Epioblasma brevidens Endangered Yes Kentucky Bivalve Freshwater

Pearlymussel, Tubercledblossom Epioblasma torulosa torulosa Endangered No Kentucky Bivalve Freshwater

Woodpecker, Redcockaded Picoides borealis Endangered No Kentucky Bird Terrestrial

Pearlymussel, Orangefooted Plethobasus cooperianus Endangered No Kentucky Bivalve Freshwater

## 132 Organism Scientific Name Status Critical Habitat State Taxa Medium

Tern, Interior (population) Least Sterna antillarum Endangered No Kentucky Bird Terrestrial

Bat, Virginia Big-eared Corynorhinus (=Plecotus) townsendii virginianus Endangered Yes Kentucky Mammal Terrestrial, Subterraneous Pearlymussel, Dromedary Dromus dromas Endangered No Kentucky Bivalve Freshwater Mucket, Pink (Pearlymussel) Lampsilis abrupta Endangered No Kentucky Bivalve Freshwater

Pearlymussel, Fat Pocketbook Potamilus capax Endangered No Kentucky Bivalve Freshwater

Riffleshell, Northern Epioblasma torulosa rangiana Endangered No Kentucky Bivalve Freshwater

Pearlymussel, Cumberland Bean Villosa trabalis Endangered No Kentucky Bivalve Freshwater

Darter, Bluemask (=jewel) Etheostoma / Endangered No Kentucky Fish Freshwater

Pearlymussel, Cracking Hemistena lata Endangered No Kentucky Bivalve Freshwater Mussel, Ring Pink (=Golf Stick Pearly) Obovaria retusa Endangered No Kentucky Bivalve Freshwater

Mussel, Clubshell Pleurobema clava Endangered No Kentucky Bivalve Freshwater Bat, Indiana Myotis sodalis Endangered Yes Kentucky Mammal Subterraneous, Terrestrial Plover, Piping Charadrius melodus Endangered Yes Kentucky Bird TerrestrialPearlymussel, Yellowblossom Epioblasma florentina florentina Endangered No Kentucky Bivalve Freshwater

Mussel, Oyster Epioblasma capsaeformis Endangered Yes Kentucky Bivalve Freshwater Pearlymussel, White Wartyback Plethobasus cicatricosus Endangered No Kentucky Bivalve Freshwater

Darter, Relict Etheostoma chienense Endangered No Kentucky Fish Freshwater Dace, Blackside Phoxinus cumberlandensis Threatened No Kentucky Fish Freshwater Shrimp, Kentucky Cave Palaemonias ganteri Endangered Yes Kentucky Crustacean Freshwater Eagle, Bald Haliaeetus leucocephalus Threatened No Kentucky Bird Terrestrial

Fanshell Cyprogenia stegaria Endangered No Kentucky Bivalve Freshwater Shiner, Palezone Notropis albizonatus Endangered No Kentucky Fish Freshwater Mussel, Rough Pigtoe Pleurobema plenum Endangered No Kentucky Bivalve Freshwater Tern, Interior (population) Least Sterna antillarum Endangered No Louisiana Bird Terrestrial

Tortoise, Gopher Gopherus polyphemus Threatened No Louisiana Reptile Terrestrial Sea turtle, hawksbill Eretmochelys imbricata Endangered Yes Louisiana Reptile Saltwater Plover, Piping Charadrius melodus Endangered Yes Louisiana Bird Terrestrial Sturgeon, Pallid Scaphirhynchus albus Endangered No Louisiana Fish Freshwater

Mucket, Pink (Pearlymussel) Lampsilis abrupta Endangered No Louisiana Bivalve Freshwater

Pelican, Brown Pelecanus occidentalis Endangered No Louisiana Bird Terrestrial Sea turtle, leatherback Dermochelys coriacea Endangered Yes Louisiana Reptile Saltwater Eagle, Bald Haliaeetus leucocephalus Threatened No Louisiana Bird Terrestrial Sea turtle, green Chelonia mydas Endangered No Louisiana Reptile Saltwater Pearlshell, Louisiana Margaritifera hembeli Threatened No Louisiana Bivalve Freshwater

## 133 Organism Scientific Name Status Critical Habitat State Taxa Medium

Turtle, Ringed Sawback Graptemys oculifera Threatened No Louisiana Reptile Freshwater, Terrestrial Sea turtle, Kemp's ridley Lepidochelys kempii Endangered No Louisiana Reptile Saltwater

Bear, Louisiana Black Ursus americanus luteolus Threatened No Louisiana Mammal Terrestrial

Woodpecker, Redcockaded Picoides borealis Endangered No Louisiana Bird Terrestrial

Mussel, Heelsplitter Inflated Potamilus inflatus Threatened No Louisiana Bivalve Freshwater

Sturgeon, Gulf Acipenser oxyrinchus desotoi Threatened Yes Louisiana Fish Saltwater, Freshwater Sea turtle, loggerhead Caretta caretta Threatened No Louisiana Reptile Saltwater

Salmon, Atlantic Salmo salar Endangered No Maine Fish Brackish, Saltwater, Freshwater Eagle, Bald Haliaeetus leucocephalus Threatened No Maine Bird Terrestrial Lynx, Canada Lynx canadensis Threatened No Maine Mammal Terrestrial Sturgeon, Shortnose Acipenser brevirostrum Endangered No Maine Fish Saltwater, Freshwater Tern, Roseate Sterna dougallii dougallii Endangered No Maine Bird Terrestrial Plover, Piping Charadrius melodus Endangered Yes Maine Bird Terrestrial Turtle, Bog (Northern population) Clemmys muhlenbergii Threatened No Maryland Reptile Terrestrial, Freshwater Darter, Maryland Etheostoma sellare Endangered Yes Maryland Fish Freshwater Squirrel, Delmarva

Peninsula Fox Sciurus niger cinereus Endangered No Maryland Mammal Terrestrial

Beetle, Northeastern Beach Tiger Cicindela dorsalis dorsalis Threatened No Maryland Insect Terrestrial

Mussel, Dwarf Wedge Alasmidonta heterodon Endangered No Maryland Bivalve Freshwater Eagle, Bald Haliaeetus leucocephalus Threatened No Maryland Bird Terrestrial Beetle, Puritan Tiger Cicindela puritana Threatened No Maryland Insect Terrestrial, Coastal (neritic) Plover, Piping Charadrius melodus Endangered Yes Maryland Bird Terrestrial Sturgeon, Shortnose Acipenser brevirostrum Endangered No Maryland Fish Saltwater, Freshwater Bat, Indiana Myotis sodalis Endangered Yes Maryland Mammal Subterraneous, Terrestrial Beetle, Northeastern Beach Tiger Cicindela dorsalis dorsalis Threatened No Massachusetts Insect Terrestrial

Tern, Roseate Sterna dougallii dougallii Endangered No Massachusetts Bird Terrestrial Beetle, American Burying Nicrophorus americanus Endangered No Massachusetts Insect Terrestrial

Starling, Ponape Mountain Aplonis pelzelni Endangered No Massachusetts Bird Terrestrial

Sturgeon, Shortnose Acipenser brevirostrum Endangered No Massachusetts Fish Saltwater, Freshwater Bat, Indiana Myotis sodalis Endangered Yes Massachusetts Mammal Subterraneous, Terrestrial Plover, Piping Charadrius melodus Endangered Yes Massachusetts Bird Terrestrial

## 134 Organism Scientific Name Status Critical Habitat State Taxa Medium

Turtle, Bog (Northern population) Clemmys muhlenbergii Threatened No Massachusetts Reptile Terrestrial, Freshwater Beetle, Puritan Tiger Cicindela puritana Threatened No Massachusetts Insect Terrestrial, Coastal (neritic) Eagle, Bald Haliaeetus leucocephalus Threatened No Massachusetts Bird TerrestrialTurtle, Plymouth Redbellied Pseudemys rubriventris bangsi Endangered Yes Massachusetts Reptile Terrestrial, Freshwater Beetle, Hungerford's

Crawling Water Brychius hungerfordi Endangered No Michigan Insect Freshwater

Butterfly, Mitchell's Satyr Neonympha mitchellii mitchellii Endangered No Michigan Insect Terrestrial

Eagle, Bald Haliaeetus leucocephalus Threatened No Michigan Bird Terrestrial Dragonfly, Hine's Emerald Somatochlora hineana Endangered No Michigan Insect Freshwater, Terrestrial Butterfly, Karner Blue Lycaeides melissa samuelis Endangered No Michigan Insect Terrestrial

Lynx, Canada Lynx canadensis Threatened No Michigan Mammal Terrestrial Riffleshell, Northern Epioblasma torulosa rangiana Endangered No Michigan Bivalve Freshwater

Mussel, Clubshell Pleurobema clava Endangered No Michigan Bivalve Freshwater Bat, Indiana Myotis sodalis Endangered Yes Michigan Mammal Subterraneous, Terrestrial Plover, Piping Charadrius melodus Endangered Yes Michigan Bird Terrestrial Warbler (=Wood), Kirtland's Dendroica kirtlandii Endangered No Michigan Bird Terrestrial

Snake, Northern Copperbelly Water Nerodia erythrogaster neglecta Threatened No Michigan Reptile Freshwater, Terrestrial Wolf, Gray Canis lupus Endangered Yes Michigan Mammal Terrestrial Butterfly, Karner Blue Lycaeides melissa samuelis Endangered No Minnesota Insect Terrestrial

Mussel, Winged Mapleleaf Quadrula fragosa Endangered No Minnesota Bivalve Freshwater

Plover, Piping Charadrius melodus Endangered Yes Minnesota Bird Terrestrial Shiner, Topeka Notropis topeka (=tristis) Endangered Yes Minnesota Fish Freshwater Pearlymussel, Higgins' Eye Lampsilis higginsii Endangered No Minnesota Bivalve Freshwater

Lynx, Canada Lynx canadensis Threatened No Minnesota Mammal Terrestrial

Wolf, Gray Canis lupus Threatened Yes Minnesota Mammal Terrestrial Eagle, Bald Haliaeetus leucocephalus Threatened No Minnesota Bird Terrestrial Tortoise, Gopher Gopherus polyphemus Threatened No Mississippi Reptile Terrestrial Mussel, Alabama

Moccasinshell Medionidus acutissimus Threatened Yes Mississippi Bivalve Freshwater

Woodpecker, Redcockaded Picoides borealis Endangered No Mississippi Bird Terrestrial

Combshell, Southern

(=Penitent mussel) Epioblasma penita Endangered No Mississippi Bivalve Freshwater

Darter, Bayou Etheostoma rubrum Threatened No Mississippi Fish Freshwater Sea turtle, green Chelonia mydas Endangered No Mississippi Reptile Saltwater

## 135 Organism Scientific Name Status Critical Habitat State Taxa Medium

Frog, Dusky Gopher

(Mississippi DPS) Rana capito sevosa Endangered No Mississippi Amphibian Terrestrial, Freshwater Mussel, Ovate Clubshell Pleurobema perovatum Endangered Yes Mississippi Bivalve Freshwater Sea turtle, Kemp's ridley Lepidochelys kempii Endangered No Mississippi Reptile Saltwater

Sea turtle, loggerhead Caretta caretta Threatened No Mississippi Reptile Saltwater Mucket, Orangenacre Lampsilis perovalis Threatened Yes Mississippi Bivalve Freshwater Turtle, Yellow-blotched Map Graptemys flavimaculata Threatened No Mississippi Reptile Freshwater, Terrestrial Mussel, Heelsplitter Inflated Potamilus inflatus Threatened No Mississippi Bivalve Freshwater

Turtle, Ringed Sawback Graptemys oculifera Threatened No Mississippi Reptile Freshwater, Terrestrial Bear, Louisiana Black Ursus americanus luteolus Threatened No Mississippi Mammal Terrestrial Mussel, Heavy Pigtoe (=Judge Tait's Mussel) Pleurobema taitianum Endangered No Mississippi Bivalve Freshwater

Crane, Mississippi Sandhill Grus canadensis pulla Endangered Yes Mississippi Bird Terrestrial, Freshwater Eagle, Bald Haliaeetus leucocephalus Threatened No Mississippi Bird Terrestrial Mussel, Black (=Curtus' Mussel) Clubshell Pleurobema curtum Endangered No Mississippi Bivalve Freshwater

Snake, Eastern Indigo Drymarchon corais couperi Threatened No Mississippi Reptile Terrestrial

Bat, Indiana Myotis sodalis Endangered Yes Mississippi Mammal Subterraneous, Terrestrial Tern, Interior (population) Least Sterna antillarum Endangered No Mississippi Bird Terrestrial

Bat, Gray Myotis grisescens Endangered No Mississippi Mammal Subterraneous, Terrestrial Sturgeon, Gulf Acipenser oxyrinchus desotoi Threatened Yes Mississippi Fish Saltwater, Freshwater Sturgeon, Pallid Scaphirhynchus albus Endangered No Mississippi Fish Freshwater Plover, Piping Charadrius melodus Endangered Yes Mississippi Bird Terrestrial Mussel, Southern Clubshell Pleurobema decisum Endangered Yes Mississippi Bivalve Freshwater

Pearlymussel, Fat Pocketbook Potamilus capax Endangered No Mississippi Bivalve Freshwater

Pelican, Brown Pelecanus occidentalis Endangered No Mississippi Bird Terrestrial Sturgeon, Pallid Scaphirhynchus albus Endangered No Missouri Fish Freshwater Pearlymussel, Higgins' Eye Lampsilis higginsii Endangered No Missouri Bivalve Freshwater

Dragonfly, Hine's Emerald Somatochlora hineana Endangered No Missouri Insect Freshwater, Terrestrial Mussel, Winged Mapleleaf Quadrula fragosa Endangered No Missouri Bivalve Freshwater

Beetle, American Burying Nicrophorus americanus Endangered No Missouri Insect Terrestrial

Pearlymussel, Curtis' Epioblasma florentina curtisii Endangered No Missouri Bivalve Freshwater

## 136 Organism Scientific Name Status Critical Habitat State Taxa Medium

Tern, Interior (population) Least Sterna antillarum Endangered No Missouri Bird Terrestrial

Cavefish, Ozark Amblyopsis rosae Threatened No Missouri Fish Freshwater Cavesnail, Tumbling Creek Antrobia culveri Endangered No Missouri Gastropod Subterraneous, Freshwater Bat, Indiana Myotis sodalis Endangered Yes Missouri Mammal Subterraneous, Terrestrial Eagle, Bald Haliaeetus leucocephalus Threatened No Missouri Bird Terrestrial Shiner, Topeka Notropis topeka (=tristis) Endangered Yes Missouri Fish Freshwater Madtom, Neosho Noturus placidus Threatened No Missouri Fish Freshwater Chub, Humpback Gila cypha Endangered Yes Missouri Fish Freshwater

Mucket, Pink (Pearlymussel) Lampsilis abrupta Endangered No Missouri Bivalve Freshwater

Crayfish, Cave (Cambarus aculabrum) Cambarus aculabrum Endangered No Missouri Crustacean Freshwater

Bat, Gray Myotis grisescens Endangered No Missouri Mammal Subterraneous, Terrestrial Darter, Niangua Etheostoma nianguae Threatened Yes Missouri Fish Freshwater Pearlymussel, Fat Pocketbook Potamilus capax Endangered No Missouri Bivalve Freshwater

Plover, Piping Charadrius melodus Endangered Yes Missouri Bird Terrestrial Sturgeon, Gulf Acipenser oxyrinchus desotoi Threatened Yes Missouri Fish Saltwater, Freshwater Mussel, Scaleshell Leptodea leptodon Endangered No Missouri Bivalve Freshwater

Plover, Piping Charadrius melodus Endangered Yes Montana Bird Terrestrial Bear, Grizzly Ursus arctos horribilis Threatened No Montana Mammal Terrestrial Wolf, Gray Canis lupus Endangered Yes Montana Mammal Terrestrial Ferret, Black-footed Mustela nigripes Endangered No Montana Mammal Terrestrial

Crane, Whooping Grus americana Endangered Yes Montana Bird Terrestrial, Freshwater Trout, Bull Salvelinus confluentus Threatened No Montana Fish Freshwater Trout, Bull (Columbia

River population) Salvelinus confluentus Threatened Yes Montana Fish Freshwater

Trout, Bull (Klamath

River population) Salvelinus confluentus Threatened Yes Montana Fish Freshwater

Sturgeon, White Acipenser transmontanus Endangered Yes Montana Fish Saltwater, Freshwater Sturgeon, Pallid Scaphirhynchus albus Endangered No Montana Fish Freshwater

Eagle, Bald Haliaeetus leucocephalus Threatened No Montana Bird Terrestrial Tern, Interior (population) Least Sterna antillarum Endangered No Montana Bird Terrestrial

Plover, Piping Charadrius melodus Endangered Yes Nebraska Bird Terrestrial Beetle, Salt Creek Tiger Cicindela nevadica lincolniana Endangered No Nebraska Insect Terrestrial

Shiner, Topeka Notropis topeka (=tristis) Endangered Yes Nebraska Fish Freshwater

Eagle, Bald Haliaeetus leucocephalus Threatened No Nebraska Bird Terrestrial

## 137 Organism Scientific Name Status Critical Habitat State Taxa Medium

Crane, Whooping Grus americana Endangered Yes Nebraska Bird Terrestrial, Freshwater Sturgeon, Pallid Scaphirhynchus albus Endangered No Nebraska Fish Freshwater

Tern, Interior (population) Least Sterna antillarum Endangered No Nebraska Bird Terrestrial

Ferret, Black-footed Mustela nigripes Endangered No Nebraska Mammal Terrestrial

Sucker, Warner Catostomus warnerensis Threatened Yes Nevada Fish Freshwater Pupfish, Warm Springs Cyprinodon nevadensis pectoralis Endangered No Nevada Fish Freshwater

Dace, Ash Meadows Speckled Rhinichthys osculus nevadensis Endangered Yes Nevada Fish Freshwater

Springfish, White River Crenichthys baileyi baileyi Endangered Yes Nevada Fish Freshwater

Woundfin Plagopterus argentissimus Endangered Yes Nevada Fish Freshwater Dace, Moapa Moapa coriacea Endangered No Nevada Fish Freshwater Naucorid, Ash Meadows Ambrysus amargosus Threatened Yes Nevada Insect Terrestrial Dace, Desert Eremichthys acros Threatened Yes Nevada Fish Freshwater Dace, Independence

Valley Speckled Rhinichthys osculus lethoporus Endangered No Nevada Fish Freshwater

Springfish, Hiko White River Crenichthys baileyi grandis Endangered Yes Nevada Fish Freshwater

Springfish, Railroad Valley Crenichthys nevadae Threatened Yes Nevada Fish Freshwater

Eagle, Bald Haliaeetus leucocephalus Threatened No Nevada Bird Terrestrial Spinedace, Big Spring Lepidomeda mollispinis pratensis Threatened Yes Nevada Fish Freshwater

Tortoise, Desert Gopherus agassizii Threatened Yes Nevada Reptile Terrestrial Flycatcher, Southwestern Willow Empidonax traillii extimus Endangered Yes Nevada Bird Terrestrial

Trout, Lahontan Cutthroat Oncorhynchus clarki henshawi Threatened No Nevada Fish Freshwater

Chub, Virgin River Gila seminuda (=robusta) Endangered Yes Nevada Fish Freshwater Pupfish, Ash Meadows Amargosa Cyprinodon nevadensis mionectes Endangered Yes Nevada Fish Freshwater

Spinedace, White River Lepidomeda albivallis Endangered Yes Nevada Fish Freshwater

Sucker, Razorback Xyrauchen texanus Endangered Yes Nevada Fish Freshwater Chub, Pahranagat Roundtail Gila robusta jordani Endangered No Nevada Fish Freshwater

Poolfish, Pahrump (=

Pahrump Killifish) Empetrichthys latos Endangered No Nevada Fish Freshwater

Dace, Clover Valley Speckled Rhinichthys osculus oligoporus Endangered No Nevada Fish Freshwater

Rail, Yuma Clapper Rallus longirostris yumanensis Endangered No Nevada Bird Terrestrial

Chub, Bonytail Gila elegans Endangered Yes Nevada Fish Freshwater Skipper, Carson

Wandering Pseudocopaeodes eunus obscurus Endangered No Nevada Insect Terrestrial

Trout, Bull Salvelinus confluentus Threatened No Nevada Fish Freshwater

## 138 Organism Scientific Name Status Critical Habitat State Taxa Medium

Pupfish, Devils Hole Cyprinodon diabolis Endangered No Nevada Fish Freshwater

Cui-ui Chasmistes cujus Endangered No Nevada Fish Freshwater Butterfly, Karner Blue Lycaeides melissa samuelis Endangered No New Hampshire Insect Terrestrial

Mussel, Dwarf Wedge Alasmidonta heterodon Endangered No New Hampshire Bivalve Freshwater

Bat, Indiana Myotis sodalis Endangered Yes New Hampshire Mammal Subterraneous, Terrestrial Eagle, Bald Haliaeetus leucocephalus Threatened No New Hampshire Bird Terrestrial

Eagle, Bald Haliaeetus leucocephalus Threatened No New Jersey Bird Terrestrial Plover, Piping Charadrius melodus Endangered Yes New Jersey Bird Terrestrial Sturgeon, Shortnose Acipenser brevirostrum Endangered No New Jersey Fish Saltwater, Freshwater Turtle, Bog (Northern population) Clemmys muhlenbergii Threatened No New Jersey Reptile Terrestrial, Freshwater Curlew, Eskimo Numenius borealis Endangered No New Jersey Bird Terrestrial

Bat, Indiana Myotis sodalis Endangered Yes New Jersey Mammal Subterraneous, Terrestrial Flycatcher, Southwestern Willow Empidonax traillii extimus Endangered Yes New Mexico Bird Terrestrial

Ferret, Black-footed Mustela nigripes Endangered No New Mexico Mammal Terrestrial Chub, Gila Gila intermedia Endangered Yes New Mexico Fish Freshwater Springsnail, Roswell Pyrgulopsis roswellensis Endangered No New Mexico Gastropod Freshwater

Crane, Whooping Grus americana Endangered Yes New Mexico Bird Terrestrial, Freshwater Shiner, Arkansas River Notropis girardi Threatened Yes New Mexico Fish Freshwater Wolf, Gray Canis lupus Endangered Yes New Mexico Mammal Terrestrial Shiner, Beautiful Cyprinella formosa Threatened Yes New Mexico Fish Freshwater Isopod, Socorro Thermosphaeroma thermophilus Endangered No New Mexico Crustacean Freshwater

Springsnail, Alamosa Tryonia alamosae Endangered No New Mexico Gastropod Freshwater

Gambusia, Pecos Gambusia nobilis Endangered No New Mexico Fish Freshwater Chub, Chihuahua Gila nigrescens Threatened No New Mexico Fish Freshwater Amphipod, Noel's Gammarus desperatus Endangered No New Mexico Crustacean Freshwater

Tern, Interior (population) Least Sterna antillarum Endangered No New Mexico Bird Terrestrial

Snail, Pecos Assiminea Assiminea pecos Endangered Yes New Mexico Gastropod Freshwater Jaguar Panthera onca Endangered No New Mexico Mammal Terrestrial Shiner, Pecos Bluntnose Notropis simus pecosensis Threatened Yes New Mexico Fish Freshwater Frog, Chiricahua Leopard Rana chiricahuensis Threatened No New Mexico Amphibian Freshwater, Terrestrial Sucker, Razorback Xyrauchen texanus Endangered Yes New Mexico Fish Freshwater Spikedace Meda fulgida Threatened Yes New Mexico Fish Freshwater Springsnail, Socorro Pyrgulopsis neomexicana Endangered No New Mexico Gastropod Freshwater Minnow, Rio Grande Silvery Hybognathus amarus Endangered Yes New Mexico Fish Freshwater

## 139 Organism Scientific Name Status Critical Habitat State Taxa Medium

Rattlesnake, New Mexican Ridge-nosed Crotalus willardi obscurus Threatened Yes New Mexico Reptile Terrestrial

Minnow, Loach Tiaroga cobitis Threatened Yes New Mexico Fish Freshwater

Trout, Gila Oncorhynchus gilae Endangered No New Mexico Fish Freshwater Topminnow, Gila (Yaqui) Poeciliopsis o ccidentalis Endangered No New Mexico Fish Freshwater

Owl, Mexican Spotted Strix occidentalis lucida Threatened Yes New Mexico Bird Terrestrial

Falcon, Northern Aplomado Falco femoralis septentrionalis Endangered No New Mexico Bird Terrestrial

Eagle, Bald Haliaeetus leucocephalus Threatened No New Mexico Bird Terrestrial Bat, Mexican Long-nosed Leptonycteris nivalis Endangered No New Mexico Mammal Subterraneous, Terrestrial Bat, Lesser (=Sanborn's) Long-nosed Leptonycteris curasoae yerbabuenae Endangered No New Mexico Mammal Subterraneous, Terrestrial Plover, Piping Charadrius melodus Endangered Yes New Mexico Bird Terrestrial Squawfish, Colorado Ptychocheilus lucius Endangered Yes New Mexico Fish Freshwater Springsnail, Koster's Juturnia kosteri Endangered No New Mexico Gastropod Terrestrial Bat, Indiana Myotis sodalis Endangered Yes New York Mammal Subterraneous, Terrestrial Butterfly, Karner Blue Lycaeides melissa samuelis Endangered No New York Insect Terrestrial

Turtle, Bog (Northern population) Clemmys muhlenbergii Threatened No New York Reptile Terrestrial, Freshwater Mussel, Dwarf Wedge Alasmidonta heterodon Endangered No New York Bivalve Freshwater Tern, Roseate Sterna dougallii dougallii Endangered No New York Bird Terrestrial Snail, Chittenango Ovate Amber Succinea chittenangoensis Threatened No New York Gastropod Terrestrial, Freshwater Eagle, Bald Haliaeetus leucocephalus Threatened No New York Bird Terrestrial Sturgeon, Shortnose Acipenser brevirostrum Endangered No New York Fish Saltwater, Freshwater Plover, Piping Charadrius melodus Endangered Yes New York Bird Terrestrial Plover, Piping Charadrius melodus Endangered Yes North Carolina Bird Terrestrial

Spider, Spruce-fir Moss Microhexura montivaga Endangered Yes North Carolina Arachnid Terrestrial

Silverside, Waccamaw Menidia extensa Threatened Yes North Carolina Fish Freshwater

Bat, Virginia Big-eared Corynorhinus (=Plecotus) townsendii virginianus Endangered Yes North Carolina Mammal Terrestrial, SubterraneousWoodpecker, Redcockaded Picoides borealis Endangered No North Carolina Bird Terrestrial

Eagle, Bald Haliaeetus leucocephalus Threatened No North Carolina Bird Terrestrial

Mussel, Dwarf Wedge Alasmidonta heterodon Endangered No North Carolina Bivalve Freshwater

Sea turtle, green Chelonia mydas Endangered No North Carolina Reptile Saltwater

## 140 Organism Scientific Name Status Critical Habitat State Taxa Medium

Mussel, Oyster Epioblasma capsaeformis Endangered Yes North Carolina Bivalve Freshwater

Chub, Spotfin Erimonax monachus Threatened Yes North Carolina Fish Freshwater

Pearlymussel, Little-wing Pegias fabula Endangered No North Carolina Bivalve Freshwater

Squirrel, Carolina Northern Flying Glaucomys sabrinus coloratus Endangered No North Carolina Mammal Terrestrial

Shiner, Cape Fear Notropis mekistocholas Endangered Yes North Carolina Fish Freshwater

Bat, Gray Myotis grisescens Endangered No North Carolina Mammal Subterraneous, Terrestrial Sea turtle, loggerhead Caretta caretta Threatened No North Carolina Reptile Saltwater

Spinymussel, Tar River Elliptio steinstansana Endangered No North Carolina Bivalve Freshwater

Sturgeon, Shortnose Acipenser brevirostrum Endangered No North Carolina Fish Saltwater, Freshwater Snail, Noonday Mesodon clarki nantahala Threatened No North Carolina Gastropod Terrestrial

Mussel, Heelsplitter Carolina Lasmigona decorata Endangered Yes North Carolina Bivalve Freshwater

Spinymussel, James River Pleurobema collina Endangered No North Carolina Bivalve Freshwater

Stork, Wood Mycteria americana Endangered No North Carolina Bird Terrestrial

Elktoe, Appalachian Alasmidonta raveneliana Endangered Yes North Carolina Bivalve Freshwater

Sea turtle, Kemp's ridley Lepidochelys kempii Endangered No North Carolina Reptile Saltwater

Purple Bean Villosa perpurpurea Endangered Yes North Carolina Bivalve Freshwater

Tern, Roseate Sterna dougallii dougallii Endangered No North Carolina Bird Terrestrial

Sea turtle, hawksbill Eretmochelys imbricata Endangered Yes North Carolina Reptile Saltwater

Bat, Indiana Myotis sodalis Endangered Yes North Carolina Mammal Subterraneous, Terrestrial Butterfly, Saint Francis' Satyr Neonympha mitchellii francisci Endangered No North Carolina Insect Terrestrial

Sea turtle, leatherback Dermochelys coriacea Endangered Yes North Carolina Reptile Saltwater

Crane, Whooping Grus americana Endangered Yes North Dakota Bird Terrestrial, Freshwater Eagle, Bald Haliaeetus leucocephalus Threatened No North Dakota Bird Terrestrial Tern, Interior (population) Least Sterna antillarum Endangered No North Dakota Bird Terrestrial

Plover, Piping Charadrius melodus Endangered Yes North Dakota Bird Terrestrial Sturgeon, Pallid Scaphirhynchus albus Endangered No North Dakota Fish Freshwater

## 141 Organism Scientific Name Status Critical Habitat State Taxa Medium

Fanshell Cyprogenia stegaria Endangered No Ohio Bivalve Freshwater Bat, Indiana Myotis sodalis Endangered Yes Ohio Mammal Subterraneous, Terrestrial Dragonfly, Hine's Emerald Somatochlora hineana Endangered No Ohio Insect Freshwater, Terrestrial Snake, Northern Copperbelly Water Nerodia erythrogaster neglecta Threatened No Ohio Reptile Freshwater, Terrestrial Snake, Lake Erie Water Nerodia sipedon insularum Threatened No Ohio Reptile Terrestrial, Freshwater Eagle, Bald Haliaeetus leucocephalus Threatened No Ohio Bird Terrestrial Butterfly, Karner Blue Lycaeides melissa samuelis Endangered No Ohio Insect Terrestrial

Plover, Piping Charadrius melodus Endangered Yes Ohio Bird Terrestrial Mussel, Clubshell Pleurobema clava Endangered No Ohio Bivalve Freshwater

Mucket, Pink (Pearlymussel) Lampsilis abrupta Endangered No Ohio Bivalve Freshwater

Beetle, American Burying Nicrophorus americanus Endangered No Ohio Insect Terrestrial

Pearlymussel, White Cat's Paw Epioblasma obliquata perobliqua Endangered No Ohio Bivalve Freshwater

Bat, Gray Myotis grisescens Endangered No Ohio Mammal Subterraneous, Terrestrial Riffleshell, Northern Epioblasma torulosa rangiana Endangered No Ohio Bivalve Freshwater

Madtom, Scioto Noturus trautmani Endangered No Ohio Fish Freshwater Pearlymussel, Purple Cat's Paw Epioblasma obliquata obliquata Endangered No Ohio Bivalve Freshwater

Butterfly, Mitchell's Satyr Neonympha mitchellii mitchellii Endangered No Ohio Insect Terrestrial

Bat, Gray Myotis grisescens Endangered No Oklahoma Mammal Subterraneous, TerrestrialWoodpecker, Redcockaded Picoides borealis Endangered No Oklahoma Bird Terrestrial

Eagle, Bald Haliaeetus leucocephalus Threatened No Oklahoma Bird Terrestrial Rock-pocketbook, Ouachita (=Wheeler's pm) Arkansia wheeleri Endangered No Oklahoma Bivalve Freshwater

Bat, Ozark Big-eared Corynorhinus (=Plecotus) townsendii ingens Endangered No Oklahoma Mammal Terrestrial, Subterraneous Mussel, Scaleshell Leptodea leptodon Endangered No Oklahoma Bivalve Freshwater

Cavefish, Ozark Amblyopsis rosae Threatened No Oklahoma Fish Freshwater Beetle, American Burying Nicrophorus americanus Endangered No Oklahoma Insect Terrestrial

Curlew, Eskimo Numenius borealis Endangered No Oklahoma Bird Terrestrial

Tern, Interior (population) Least Sterna antillarum Endangered No Oklahoma Bird Terrestrial

Vireo, Black-capped Vireo atricapilla Endangered No Oklahoma Bird Terrestrial Shiner, Arkansas River Notropis girardi Threatened Yes Oklahoma Fish Freshwater

## 142 Organism Scientific Name Status Critical Habitat State Taxa Medium

Plover, Piping Charadrius melodus Endangered Yes Oklahoma Bird Terrestrial Darter, Leopard Percina pantherina Threatened Yes Oklahoma Fish Freshwater

Bat, Indiana Myotis sodalis Endangered Yes Oklahoma Mammal Subterraneous, Terrestrial Crane, Whooping Grus americana Endangered Yes Oklahoma Bird Terrestrial, Freshwater Madtom, Neosho Noturus placidus Threatened No Oklahoma Fish Freshwater

Salmon, Chum (Columbia River

population) Oncorhynchus (=Salmo) keta Threatened Yes Oregon Fish Brackish, Freshwater,

Saltwater Salmon, Chinook (Upper Willamette River) Oncorhynchus (=Salmo) tshawytscha Threatened Yes Oregon Fish Saltwater, Brackish, Freshwater Salmon, Chinook (Upper Columbia River Spring) Oncorhynchus (=Salmo) tshawytscha Endangered Yes Oregon Fish Freshwater, Saltwater, Brackish Salmon, Chinook (Snake

River spring/summer) Oncorhynchus (=Salmo) tshawytscha Threatened Yes Oregon Fish Brackish, Saltwater, Freshwater Salmon, Chinook (Snake River Fall Run) Oncorhynchus (=Salmo) tshawytscha Threatened No Oregon Fish Freshwater, Saltwater, Brackish Chub, Oregon Oregonichthys crameri Endangered No Oregon Fish Freshwater Salmon, Chinook (Lower Columbia River) Oncorhynchus (=Salmo) tshawytscha Threatened Yes Oregon Fish Freshwater, Brackish, Saltwater Sucker, Warner Catostomus warnerensis Threatened Yes Oregon Fish Freshwater Butterfly, Fender's Blue Icaricia icarioides fenderi Endangered No Oregon Insect Terrestrial Plover, Western Snowy Charadrius alexandrinus nivosus Threatened Yes Oregon Bird Terrestrial

Chub, Hutton Tui Gila bicolor ssp. Threatened No Oregon Fish FreshwaterDeer, Columbian Whitetailed Odocoileus virginianus leucurus Endangered No Oregon Mammal Terrestrial

Sucker, Lost River Deltistes luxatus Endangered No Oregon Fish Freshwater Butterfly, Oregon Silverspot Speyeria zerene hippolyta Threatened Yes Oregon Insect Terrestrial

Owl, Northern Spotted Strix occidentalis caurina Threatened Yes Oregon Bird Terrestrial

Sucker, Shortnose Chasmistes brevirostris Endangered No Oregon Fish Freshwater

Pelican, Brown Pelecanus occidentalis Endangered No Oregon Bird Terrestrial Trout, Bull (Columbia

River population) Salvelinus confluentus Threatened Yes Oregon Fish Freshwater

Steelhead, (Upper Columbia River

population) Oncorhynchus (=Salmo) mykiss Threatened Yes Oregon Fish Brackish, Saltwater, Freshwater

Dace, Foskett Speckled Rhinichthys osculus ssp. Threatened No Oregon Fish Freshwater

Murrelet, Marbled Brachyramphus

marmoratus marmoratus Threatened Yes Oregon Bird Freshwater, Terrestrial, Saltwater Salmon, Sockeye (Snake River population) Oncorhynchus (=Salmo) nerka Endangered No Oregon Fish Brackish, Saltwater, Freshwater Steelhead, (Lower Columbia River

population) Oncorhynchus (=Salmo) mykiss Threatened Yes Oregon Fish Brackish, Freshwater,

Saltwater

## 143 Organism Scientific Name Status Critical Habitat State Taxa Medium

Trout, Bull Salvelinus confluentus Threatened No Oregon Fish Freshwater Salmon, Coho (Southern OR/Northern CA Coast) Oncorhynchus (=Salmo) kisutch Threatened Yes Oregon Fish Freshwater, Brackish, Saltwater Fairy Shrimp, Vernal Pool Branchinecta lynchi Threatened Yes Oregon Crustacean Vernal pool

Steelhead, (Snake River Basin population) Oncorhynchus (=Salmo) mykiss Threatened Yes Oregon Fish Freshwater, Brackish, Saltwater Trout, Bull (Klamath

River population) Salvelinus confluentus Threatened Yes Oregon Fish Freshwater

Eagle, Bald Haliaeetus leucocephalus Threatened No Oregon Bird Terrestrial Steelhead, (Upper Willamette River population) Oncorhynchus (=Salmo) mykiss Threatened Yes Oregon Fish Brackish, Saltwater, Freshwater

Steelhead, (Middle

Columbia River

population) Oncorhynchus (=Salmo) mykiss Threatened Yes Oregon Fish Freshwater, Saltwater, Brackish

Bat, Indiana Myotis sodalis Endangered Yes Pennsylvania Mammal Subterraneous, Terrestrial Riffleshell, Northern Epioblasma torulosa rangiana Endangered No Pennsylvania Bivalve Freshwater

Turtle, Bog (Northern population) Clemmys muhlenbergii Threatened No Pennsylvania Reptile Terrestrial, Freshwater Squirrel, Delmarva

Peninsula Fox Sciurus niger cinereus Endangered No Pennsylvania Mammal Terrestrial

Plover, Piping Charadrius melodus Endangered Yes Pennsylvania Bird Terrestrial

Eagle, Bald Haliaeetus leucocephalus Threatened No Pennsylvania Bird Terrestrial Mussel, Clubshell Pleurobema clava Endangered No Pennsylvania Bivalve Freshwater Beetle, American Burying Nicrophorus americanus Endangered No Rhode Island Insect Terrestrial

Plover, Piping Charadrius melodus Endangered Yes Rhode Island Bird Terrestrial Bat, Indiana Myotis sodalis Endangered Yes Rhode Island Mammal Subterraneous, Terrestrial Sturgeon, Shortnose Acipenser brevirostrum Endangered No Rhode Island Fish Saltwater, FreshwaterWoodpecker, Redcockaded Picoides borealis Endangered No South Carolina Bird Terrestrial

Bat, Indiana Myotis sodalis Endangered Yes South Carolina Mammal Subterraneous, Terrestrial Plover, Piping Charadrius melodus Endangered Yes South Carolina Bird Terrestrial

Sturgeon, Shortnose Acipenser brevirostrum Endangered No South Carolina Fish Saltwater, Freshwater Warbler, Bachman's Vermivora bachmanii Endangered No South Carolina Bird Terrestrial

Eagle, Bald Haliaeetus leucocephalus Threatened No South Carolina Bird Terrestrial

Mussel, Heelsplitter Carolina Lasmigona decorata Endangered Yes South Carolina Bivalve Freshwater

## 144 Organism Scientific Name Status Critical Habitat State Taxa Medium

Sea turtle, loggerhead Caretta caretta Threatened No South Carolina Reptile Saltwater

Snake, Eastern Indigo Drymarchon corais couperi Threatened No South Carolina Reptile Terrestrial

Sea turtle, green Chelonia mydas Endangered No South Carolina Reptile Saltwater

Salamander, Flatwoods Ambystoma cingulatum Threatened No South Carolina Amphibian Freshwater, Vernal pool, Terrestrial Sea turtle, Kemp's ridley Lepidochelys kempii Endangered No South Carolina Reptile Saltwater

Sea turtle, leatherback Dermochelys coriacea Endangered Yes South Carolina Reptile Saltwater

Stork, Wood Mycteria americana Endangered No South Carolina Bird Terrestrial

Beetle, American Burying Nicrophorus americanus Endangered No South Dakota Insect Terrestrial

Ferret, Black-footed Mustela nigripes Endangered No South Dakota Mammal Terrestrial

Sturgeon, Pallid Scaphirhynchus albus Endangered No South Dakota Fish Freshwater

Tern, Interior (population) Least Sterna antillarum Endangered No South Dakota Bird Terrestrial

Crane, Whooping Grus americana Endangered Yes South Dakota Bird Terrestrial, Freshwater Eagle, Bald Haliaeetus leucocephalus Threatened No South Dakota Bird Terrestrial Shiner, Topeka Notropis topeka (=tristis) Endangered Yes South Dakota Fish Freshwater Plover, Piping Charadrius melodus Endangered Yes South Dakota Bird Terrestrial

Bat, Gray Myotis grisescens Endangered No Tennessee Mammal Subterraneous, Terrestrial Chub, Slender Erimystax cahni Threatened Yes Tennessee Fish Freshwater Combshell, Upland Epioblasma metastriata Endangered Yes Tennessee Bivalve Freshwater Darter, Bluemask (=jewel) Etheostoma / Endangered No Tennessee Fish Freshwater

Darter, Amber Percina antesella Endangered Yes Tennessee Fish Freshwater Crayfish, Nashville Orconectes shoupi Endangered No Tennessee Crustacean Freshwater

Chub, Spotfin Erimonax monachus Threatened Yes Tennessee Fish Freshwater Dace, Blackside Phoxinus cumberlandensis Threatened No Tennessee Fish Freshwater

Bat, Indiana Myotis sodalis Endangered Yes Tennessee Mammal Subterraneous, Terrestrial Darter, Boulder Etheostoma wapiti Endangered No Tennessee Fish Freshwater Darter, Duskytail Etheostoma percnurum Endangered No Tennessee Fish Freshwater

Mussel, Coosa Moccasinshell Medionidus parvulus Endangered Yes Tennessee Bivalve Freshwater

Madtom, Pygmy Noturus stanauli Endangered No Tennessee Fish Freshwater Mussel, Ring Pink (=Golf Stick Pearly) Obovaria retusa Endangered No Tennessee Bivalve Freshwater

Mussel, Oyster Epioblasma capsaeformis Endangered Yes Tennessee Bivalve Freshwater Mussel, Ovate Clubshell Pleurobema perovatum Endangered Yes Tennessee Bivalve Freshwater

Riversnail, Anthony's Athearnia anthonyi Endangered No Tennessee Gastropod Freshwater

## 145 Organism Scientific Name Status Critical Habitat State Taxa Medium

Mussel, Fine-rayed Pigtoe Fusconaia cuneolus Endangered No Tennessee Bivalve Freshwater

Mussel, Fine-lined Pocketbook Lampsilis altilis Threatened Yes Tennessee Bivalve Freshwater

Mussel, Cumberland Pigtoe Pleurobema gibberum Endangered No Tennessee Bivalve Freshwater

Riffleshell, Tan Epioblasma florentina walkeri (=E. walkeri) Endangered No Tennessee Bivalve Freshwater

Mussel, Cumberland Combshell Epioblasma brevidens Endangered Yes Tennessee Bivalve Freshwater

Mussel, Shiny Pigtoe Fusconaia cor Endangered No Tennessee Bivalve Freshwater

Mussel, Clubshell Pleurobema clava Endangered No Tennessee Bivalve Freshwater Pearlymussel, Dromedary Dromus dromas Endangered No Tennessee Bivalve Freshwater Sturgeon, Pallid Scaphirhynchus albus Endangered No Tennessee Fish Freshwater

Stork, Wood Mycteria americana Endangered No Tennessee Bird Terrestrial Mucket, Pink (Pearlymussel) Lampsilis abrupta Endangered No Tennessee Bivalve Freshwater

Marstonia, Royal

(=Royal Snail) Pyrgulopsis ogmorhaphe Endangered No Tennessee Gastropod Terrestrial

Madtom, Yellowfin Noturus flavipinnis Threatened Yes Tennessee Fish Freshwater

Madtom, Smoky Noturus baileyi Endangered Yes Tennessee Fish Freshwater Mussel, Cumberland Elktoe Alasmidonta atropurpurea Endangered Yes Tennessee Bivalve Freshwater

Pearlymussel, Cumberland Monkeyface Quadrula intermedia Endangered No Tennessee Bivalve Freshwater

Pearlymussel, Tubercledblossom Epioblasma torulosa torulosa Endangered No Tennessee Bivalve Freshwater

Pearlymussel, Purple Cat's Paw Epioblasma obliquata obliquata Endangered No Tennessee Bivalve Freshwater

Pearlymussel, Pale Lilliput Toxolasma cylindrellus Endangered No Tennessee Bivalve Freshwater

Pearlymussel, Orangefooted Plethobasus cooperianus Endangered No Tennessee Bivalve Freshwater

Pearlymussel, White Wartyback Plethobasus cicatricosus Endangered No Tennessee Bivalve Freshwater

Pearlymussel, Little-wing Pegias fabula Endangered No Tennessee Bivalve Freshwater Tern, Interior (population) Least Sterna antillarum Endangered No Tennessee Bird Terrestrial

Pearlymussel, Yellowblossom Epioblasma florentina florentina Endangered No Tennessee Bivalve Freshwater

Mussel, Rough Pigtoe Pleurobema plenum Endangered No Tennessee Bivalve FreshwaterPearlymussel, Turgidblossom Epioblasma turgidula Endangered No Tennessee Bivalve Freshwater

Mussel, Alabama

Moccasinshell Medionidus acutissimus Threatened Yes Tennessee Bivalve Freshwater

Pearlymussel, Cumberland Bean Villosa trabalis Endangered No Tennessee Bivalve Freshwater

Pearlymussel, Cracking Hemistena lata Endangered No Tennessee Bivalve Freshwater

## 146 Organism Scientific Name Status Critical Habitat State Taxa Medium

Pearlymussel, Birdwing Conradilla caelata Endangered No Tennessee Bivalve Freshwater Pearlymussel, Appalachian Monkeyface Quadrula sparsa Endangered No Tennessee Bivalve Freshwater

Pearlymussel, Alabama Lamp Lampsilis virescens Endangered No Tennessee Bivalve Freshwater

Purple Bean Villosa perpurpurea Endangered Yes Tennessee Bivalve Freshwater Rabbitsfoot, Rough Quadrula cylindrica strigillata Endangered Yes Tennessee Bivalve Freshwater

Mussel, Southern Pigtoe Pleurobema georgianum Endangered Yes Tennessee Bivalve Freshwater

Pearlymussel, Greenblossom Epioblasma torulosa gubernaculum Endangered No Tennessee Bivalve Freshwater

Spider, Spruce-fir Moss Microhexura montivaga Endangered Yes Tennessee Arachnid Terrestrial

Elktoe, Appalachian Alasmidonta raveneliana Endangered Yes Tennessee Bivalve Freshwater Logperch, Conasauga Percina jenkinsi Endangered Yes Tennessee Fish Freshwater Snail, Painted Snake Coiled Forest Anguispira picta Threatened No Tennessee Gastropod Terrestrial

Shiner, Blue Cyprinella caerulea Threatened No Tennessee Fish FreshwaterWoodpecker, Redcockaded Picoides borealis Endangered No Tennessee Bird Terrestrial

Shiner, Palezone Notropis albizonatus Endangered No Tennessee Fish Freshwater

Fanshell Cyprogenia stegaria Endangered No Tennessee Bivalve Freshwater Eagle, Bald Haliaeetus leucocephalus Threatened No Tennessee Bird Terrestrial Darter, Snail Percina tanasi Threatened No Tennessee Fish Freshwater Kidneyshell, Triangular Ptychobranchus greenii Endangered Yes Tennessee Bivalve Freshwater Squirrel, Carolina Northern Flying Glaucomys sabrinus coloratus Endangered No Tennessee Mammal Terrestrial

Darter, Slackwater Etheostoma boschungi Threatened Yes Tennessee Fish Freshwater Rhadine infernalis (ncn) Rhadine infernalis Endangered Yes Texas Insect Terrestrial, Subterraneous Salamander, Texas Blind Typhlomolge rathbuni Endangered No Texas Amphibian Subterraneous, Freshwater Vireo, Black-capped Vireo atricapilla Endangered No Texas Bird Terrestrial Snake, Concho Water Nerodia paucimaculata Threatened Yes Texas Reptile Freshwater, Terrestrial Tern, Interior (population) Least Sterna antillarum Endangered No Texas Bird Terrestrial

Ocelot Leopardus (=Felis) pardalis Endangered No Texas Mammal Terrestrial

Owl, Mexican Spotted Strix occidentalis lucida Threatened Yes Texas Bird Terrestrial

Falcon, Northern Aplomado Falco femoralis septentrionalis Endangered No Texas Bird Terrestrial

Rhadine exilis (ncn) Rhadine exilis Endangered Yes Texas Insect Terrestrial, Subterraneous Warbler (=Wood),

Golden-cheeked Dendroica chrysoparia Endangered No Texas Bird Terrestrial

Beetle, Tooth Cave Ground Rhadine persephone Endangered No Texas Insect Subterraneous

## 147 Organism Scientific Name Status Critical Habitat State Taxa Medium

Spider, Government Canyon Cave Neoleptoneta microps Endangered No Texas Arachnid Subterraneous, Terrestrial Pupfish, Comanche Springs Cyprinodon elegans Endangered No Texas Fish Freshwater

Pseudoscorpion, Tooth Cave Tartarocreagris texana Endangered No Texas Arachnid Terrestrial, SubterraneousWoodpecker, Redcockaded Picoides borealis Endangered No Texas Bird Terrestrial

Prairie-chicken, Attwater's Greater Tympanuchus cupido attwateri Endangered No Texas Bird Terrestrial

Spider, Madla's Cave Cicurina madla Endangered Yes Texas Arachnid Subterraneous, Terrestrial Spider, Robber Baron Cave Cicurina baronia Endangered Yes Texas Arachnid Terrestrial, Subterraneous Plover, Piping Charadrius melodus Endangered Yes Texas Bird Terrestrial Pelican, Brown Pelecanus occidentalis Endangered No Texas Bird Terrestrial Spider, Tooth Cave Neoleptoneta myopica Endangered No Texas Arachnid Terrestrial, Subterraneous Eagle, Bald Haliaeetus leucocephalus Threatened No Texas Bird Terrestrial Spider, Vesper Cave Cicurina vespera Endangered No Texas Arachnid Subterraneous, Terrestrial Pupfish, Leon Springs Cyprinodon bovinus Endangered Yes Texas Fish Freshwater Meshweaver, Braken Bat Cave Cicurina venii Endangered Yes Texas Arachnid Terrestrial, Subterraneous Amphipod, Peck's Cave Stygobromus (=Stygonectes) pecki Endangered No Texas Crustacean Subterraneous, Freshwater Harvestman, Robber Baron Cave Texella cokendolpheri Endangered Yes Texas Arachnid Subterraneous, Terrestrial Snail, Pecos Assiminea Assiminea pecos Endangered Yes Texas Gastropod Freshwater Shiner, Arkansas River Notropis girardi Threatened Yes Texas Fish Freshwater Sea turtle, loggerhead Caretta caretta Threatened No Texas Reptile Saltwater Jaguarundi, Gulf Coast Herpailurus (=Felis) yagouaroundi cacomitli Endangered No Texas Mammal Terrestrial

Darter, Fountain Etheostoma fonticola Endangered Yes Texas Fish Freshwater Minnow, Devils River Dionda diaboli Threatened No Texas Fish Freshwater Harvestman, Bone Cave Texella reyesi Endangered No Texas Arachnid Terrestrial, Subterraneous Bat, Mexican Long-nosed Leptonycteris nivalis Endangered No Texas Mammal Subterraneous, Terrestrial Sea turtle, hawksbill Eretmochelys imbricata Endangered Yes Texas Reptile Saltwater

Crane, Whooping Grus americana Endangered Yes Texas Bird Terrestrial, Freshwater Jaguarundi, Sinaloan Herpailurus (=Felis) yagouaroundi tolteca Endangered No Texas Mammal Terrestrial

Sea turtle, Kemp's ridley Lepidochelys kempii Endangered No Texas Reptile Saltwater

Sea turtle, leatherback Dermochelys coriacea Endangered Yes Texas Reptile Saltwater Sea turtle, green Chelonia mydas Endangered No Texas Reptile Saltwater Gambusia, Clear Creek Gambusia heterochir Endangered No Texas Fish Freshwater

## 148 Organism Scientific Name Status Critical Habitat State Taxa Medium

Beetle, Kretschmarr Cave Mold Texamaurops reddelli Endangered No Texas Insect Subterraneous

Beetle, Helotes Mold Batrisodes venyivi Endangered Yes Texas Insect Subterraneous

Curlew, Eskimo Numenius borealis Endangered No Texas Bird Terrestrial Flycatcher, Southwestern Willow Empidonax traillii extimus Endangered Yes Texas Bird Terrestrial

Gambusia, Big Bend Gambusia gaigei Endangered No Texas Fish Freshwater Beetle, Comal Springs Riffle Heterelmis comalensis Endangered No Texas Insect Subterraneous, Freshwater Bear, Louisiana Black Ursus americanus luteolus Threatened No Texas Mammal Terrestrial Beetle, Coffin Cave Mold Batrisodes texanus Endangered No Texas Insect Subterraneous Toad, Houston Bufo houstonensis Endangered Yes Texas Amphibian Terrestrial, Freshwater Beetle, American Burying Nicrophorus americanus Endangered No Texas Insect Terrestrial

Gambusia, Pecos Gambusia nobilis Endangered No Texas Fish Freshwater Salamander, Barton Springs Eurycea sosorum Endangered No Texas Amphibian Freshwater, Terrestrial Gambusia, San Marcos Gambusia georgei Endangered Yes Texas Fish Freshwater Salamander, San Marcos Eurycea nana Threatened Yes Texas Amphibian Freshwater, Terrestrial Harvestman, Bee Creek Cave Texella reddelli Endangered No Texas Arachnid Terrestrial, Subterraneous Beetle, Comal Springs Dryopid Stygoparnus comalensis Endangered No Texas Insect Subterraneous, Freshwater Sucker, June Chasmistes liorus Endangered Yes Utah Fish Freshwater Prairie Dog, Utah Cynomys parvidens Threatened No Utah Mammal Terrestrial, Subterraneous Sucker, Razorback Xyrauchen texanus Endangered Yes Utah Fish Freshwater Squawfish, Colorado Ptychocheilus lucius Endangered Yes Utah Fish Freshwater

Chub, Humpback Gila cypha Endangered Yes Utah Fish Freshwater Flycatcher, Southwestern Willow Empidonax traillii extimus Endangered Yes Utah Bird Terrestrial

Chub, Bonytail Gila elegans Endangered Yes Utah Fish Freshwater

Eagle, Bald Haliaeetus leucocephalus Threatened No Utah Bird Terrestrial Owl, Mexican Spotted Strix occidentalis lucida Threatened Yes Utah Bird Terrestrial

Woundfin Plagopterus argentissimus Endangered Yes Utah Fish Freshwater Tortoise, Desert Gopherus agassizii Threatened Yes Utah Reptile Terrestrial Ferret, Black-footed Mustela nigripes Endangered No Utah Mammal Terrestrial Chub, Virgin River Gila seminuda (=robusta) Endangered Yes Utah Fish Freshwater

Trout, Lahontan Cutthroat Oncorhynchus clarki henshawi Threatened No Utah Fish Freshwater

Mussel, Dwarf Wedge Alasmidonta heterodon Endangered No Vermont Bivalve Freshwater Eagle, Bald Haliaeetus leucocephalus Threatened No Vermont Bird Terrestrial Bat, Indiana Myotis sodalis Endangered Yes Vermont Mammal Subterraneous, Terrestrial

## 149 Organism Scientific Name Status Critical Habitat State Taxa Medium

Salamander, Shenandoah Plethodon shenandoah Endangered No Virginia Amphibian Freshwater, Terrestrial Chub, Spotfin Erimonax monachus Threatened Yes Virginia Fish Freshwater Spinymussel, James River Pleurobema collina Endangered No Virginia Bivalve Freshwater

Woodpecker, Redcockaded Picoides borealis Endangered No Virginia Bird Terrestrial

Sturgeon, Shortnose Acipenser brevirostrum Endangered No Virginia Fish Saltwater, Freshwater Pearlymussel, Little-wing Pegias fabula Endangered No Virginia Bivalve Freshwater Bat, Gray Myotis grisescens Endangered No Virginia Mammal Subterraneous, Terrestrial Butterfly, Saint Francis' Satyr Neonympha mitchellii francisci Endangered No Virginia Insect Terrestrial

Chub, Slender Erimystax cahni Threatened Yes Virginia Fish Freshwater Dace, Blackside Phoxinus cumberlandensis Threatened No Virginia Fish Freshwater

Mucket, Pink (Pearlymussel) Lampsilis abrupta Endangered No Virginia Bivalve Freshwater

Bat, Virginia Big-eared Corynorhinus (=Plecotus) townsendii virginianus Endangered Yes Virginia Mammal Terrestrial, Subterraneous Snail, Virginia Fringed Mountain Polygyriscus virginianus Endangered No Virginia Gastropod Terrestrial

Fanshell Cyprogenia stegaria Endangered No Virginia Bivalve Freshwater Madtom, Yellowfin Noturus flavipinnis Threatened Yes Virginia Fish Freshwater Logperch, Roanoke Percina rex Endangered No Virginia Fish Freshwater Isopod, Madison Cave Antrolana lira Threatened No Virginia Crustacean Freshwater Sea turtle, loggerhead Caretta caretta Threatened No Virginia Reptile Saltwater Isopod, Lee County Cave Lirceus usdagalun Endangered No Virginia Crustacean Freshwater Bat, Indiana Myotis sodalis Endangered Yes Virginia Mammal Subterraneous, Terrestrial Eagle, Bald Haliaeetus leucocephalus Threatened No Virginia Bird Terrestrial Pearlymussel, Appalachian Monkeyface Quadrula sparsa Endangered No Virginia Bivalve Freshwater

Pearlymussel, Cumberland Monkeyface Quadrula intermedia Endangered No Virginia Bivalve Freshwater

Butterfly, Mitchell's Satyr Neonympha mitchellii mitchellii Endangered No Virginia Insect Terrestrial

Pearlymussel, Greenblossom Epioblasma torulosa gubernaculum Endangered No Virginia Bivalve Freshwater

Pearlymussel, Dromedary Dromus dromas Endangered No Virginia Bivalve Freshwater Plover, Piping Charadrius melodus Endangered Yes Virginia Bird Terrestrial Squirrel, Virginia Northern Flying Glaucomys sabrinus fuscus Endangered No Virginia Mammal Terrestrial

Pearlymussel, Cumberland Bean Villosa trabalis Endangered No Virginia Bivalve Freshwater

Pearlymussel, Cracking Hemistena lata Endangered No Virginia Bivalve Freshwater Pearlymussel, Birdwing Conradilla caelata Endangered No Virginia Bivalve Freshwater

## 150 Organism Scientific Name Status Critical Habitat State Taxa Medium

Mussel, Cumberland Combshell Epioblasma brevidens Endangered Yes Virginia Bivalve Freshwater

Squirrel, Delmarva

Peninsula Fox Sciurus niger cinereus Endangered No Virginia Mammal Terrestrial

Mussel, Cumberland Elktoe Alasmidonta atropurpurea Endangered Yes Virginia Bivalve Freshwater

Mussel, Oyster Epioblasma capsaeformis Endangered Yes Virginia Bivalve Freshwater Darter, Duskytail Etheostoma percnurum Endangered No Virginia Fish Freshwater

Purple Bean Villosa perpurpurea Endangered Yes Virginia Bivalve Freshwater Rabbitsfoot, Rough Quadrula cylindrica strigillata Endangered Yes Virginia Bivalve Freshwater

Beetle, Northeastern Beach Tiger Cicindela dorsalis dorsalis Threatened No Virginia Insect Terrestrial

Mussel, Shiny Pigtoe Fusconaia cor Endangered No Virginia Bivalve Freshwater Mussel, Rough Pigtoe Pleurobema plenum Endangered No Virginia Bivalve Freshwater Mussel, Dwarf Wedge Alasmidonta heterodon Endangered No Virginia Bivalve Freshwater

Mussel, Fine-rayed Pigtoe Fusconaia cuneolus Endangered No Virginia Bivalve Freshwater

Riffleshell, Tan Epioblasma florentina walkeri (=E. walkeri) Endangered No Virginia Bivalve Freshwater

Murrelet, Marbled Brachyramphus

marmoratus marmoratus Threatened Yes Washington Bird Freshwater, Terrestrial, Saltwater Steelhead, (Middle

Columbia River

population) Oncorhynchus (=Salmo) mykiss Threatened Yes Washington Fish Freshwater, Saltwater, Brackish

Steelhead, (Lower Columbia River

population) Oncorhynchus (=Salmo) mykiss Threatened Yes Washington Fish Brackish, Freshwater,

Saltwater Wolf, Gray Canis lupus Endangered Yes Washington Mammal Terrestrial Steelhead, (Upper Columbia River

population) Oncorhynchus (=Salmo) mykiss Threatened Yes Washington Fish Brackish, Saltwater, Freshwater

Eagle, Bald Haliaeetus leucocephalus Threatened No Washington Bird TerrestrialDeer, Columbian Whitetailed Odocoileus virginianus leucurus Endangered No Washington Mammal Terrestrial

Steelhead, (Upper Willamette River population) Oncorhynchus (=Salmo) mykiss Threatened Yes Washington Fish Brackish, Saltwater, Freshwater

Salmon, Chinook (Snake

River spring/summer) Oncorhynchus (=Salmo) tshawytscha Threatened Yes Washington Fish Brackish, Saltwater, Freshwater Butterfly, Oregon Silverspot Speyeria zerene hippolyta Threatened Yes Washington Insect Terrestrial

Pelican, Brown Pelecanus occidentalis Endangered No Washington Bird Terrestrial Steelhead, (Snake River Basin population) Oncorhynchus (=Salmo) mykiss Threatened Yes Washington Fish Freshwater, Brackish, Saltwater Plover, Western Snowy Charadrius alexandrinus nivosus Threatened Yes Washington Bird Terrestrial

Caribou, Woodland Rangifer tarandus caribou Endangered No Washington Mammal Terrestrial

## 151 Organism Scientific Name Status Critical Habitat State Taxa Medium

Owl, Northern Spotted Strix occidentalis caurina Threatened Yes Washington Bird Terrestrial Rabbit, Pygmy Brachylagus idahoensis Endangered No Washington Mammal Terrestrial Salmon, Chinook (Lower Columbia River) Oncorhynchus (=Salmo) tshawytscha Threatened Yes Washington Fish Freshwater, Brackish, Saltwater Salmon, Chum (Columbia River

population) Oncorhynchus (=Salmo) keta Threatened Yes Washington Fish Brackish, Freshwater,

Saltwater Salmon, Chinook (Snake River Fall Run) Oncorhynchus (=Salmo) tshawytscha Threatened No Washington Fish Freshwater, Saltwater, Brackish Steelhead, Puget Sound Oncorhynchus mykiss Threatened No Washington Fish Salmon, Chinook (Upper Columbia River Spring) Oncorhynchus (=Salmo) tshawytscha Endangered Yes Washington Fish Freshwater, Saltwater, Brackish Salmon, Chinook (Upper Willamette River) Oncorhynchus (=Salmo) tshawytscha Threatened Yes Washington Fish Saltwater, Brackish, Freshwater Bear, Grizzly Ursus arctos horribilis Threatened No Washington Mammal Terrestrial Salmon, Chum (Hood Canal Summer

population) Oncorhynchus (=Salmo) keta Threatened Yes Washington Fish Freshwater, Brackish, Saltwater

Salmon, Sockeye (Ozette Lake population) Oncorhynchus (=Salmo) nerka Threatened Yes Washington Fish Saltwater, Freshwater,

Brackish Salmon, Sockeye (Snake River population) Oncorhynchus (=Salmo) nerka Endangered No Washington Fish Brackish, Saltwater, Freshwater Trout, Bull Salvelinus confluentus Threatened No Washington Fish Freshwater Trout, Bull (Columbia

River population) Salvelinus confluentus Threatened Yes Washington Fish Freshwater

Trout, Bull (Klamath

River population) Salvelinus confluentus Threatened Yes Washington Fish Freshwater

Salmon, Chinook (Puget Sound) Oncorhynchus (=Salmo) tshawytscha Threatened Yes Washington Fish Freshwater, Brackish, Saltwater Bat, Gray Myotis grisescens Endangered No West Virginia Mammal Subterraneous, Terrestrial Salamander, Cheat Mountain Plethodon nettingi Threatened No West Virginia Amphibian Freshwater, Terrestrial

Fanshell Cyprogenia stegaria Endangered No West Virginia Bivalve Freshwater Squirrel, Virginia Northern Flying Glaucomys sabrinus fuscus Endangered No West Virginia Mammal Terrestrial

Mussel, Clubshell Pleurobema clava Endangered No West Virginia Bivalve Freshwater Eagle, Bald Haliaeetus leucocephalus Threatened No West Virginia Bird Terrestrial Mucket, Pink (Pearlymussel) Lampsilis abrupta Endangered No West Virginia Bivalve Freshwater

Bat, Virginia Big-eared Corynorhinus (=Plecotus) townsendii virginianus Endangered Yes West Virginia Mammal Terrestrial, Subterraneous Bat, Indiana Myotis sodalis Endangered Yes West Virginia Mammal Subterraneous, TerrestrialSnail, Flat-spired Threetoothed Triodopsis platysayoides Threatened No West Virginia Gastropod Terrestrial

Spinymussel, James River Pleurobema collina Endangered No West Virginia Bivalve Freshwater

## 152 Organism Scientific Name Status Critical Habitat State Taxa Medium

Squirrel, Carolina Northern Flying Glaucomys sabrinus coloratus Endangered No West Virginia Mammal Terrestrial

Pearlymussel, Tubercledblossom Epioblasma torulosa torulosa Endangered No West Virginia Bivalve Freshwater

Dragonfly, Hine's Emerald Somatochlora hineana Endangered No Wisconsin Insect Freshwater, Terrestrial Crane, Whooping Grus americana Endangered Yes Wisconsin Bird Terrestrial, Freshwater Wolf, Gray Canis lupus Endangered Yes Wisconsin Mammal Terrestrial Warbler (=Wood), Kirtland's Dendroica kirtlandii Endangered No Wisconsin Bird Terrestrial

Eagle, Bald Haliaeetus leucocephalus Threatened No Wisconsin Bird Terrestrial Butterfly, Karner Blue Lycaeides melissa samuelis Endangered No Wisconsin Insect Terrestrial

Lynx, Canada Lynx canadensis Threatened No Wisconsin Mammal Terrestrial Mussel, Winged Mapleleaf Quadrula fragosa Endangered No Wisconsin Bivalve Freshwater

Pearlymussel, Higgins' Eye Lampsilis higginsii Endangered No Wisconsin Bivalve Freshwater

Plover, Piping Charadrius melodus Endangered Yes Wisconsin Bird Terrestrial Ferret, Black-footed Mustela nigripes Endangered No Wyoming Mammal Terrestrial Eagle, Bald Haliaeetus leucocephalus Threatened No Wyoming Bird Terrestrial Toad, Wyoming Bufo baxteri (=hemiophrys) Endangered No Wyoming Amphibian Freshwater, Terrestrial Dace, Kendall Warm Springs Rhinichthys osculus thermalis Endangered No Wyoming Fish Freshwater

Wolf, Gray Canis lupus Endangered Yes Wyoming Mammal Terrestrial Dace, Moapa Moapa coriacea Endangered No Wyoming Fish Freshwater Bear, Grizzly Ursus arctos horribilis Threatened No Wyoming Mammal Terrestrial Mouse, Preble's Meadow Jumping Zapus hudsonius preblei Threatened Yes Wyoming Mammal Terrestrial