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Submission Information

Submission ID : 25419379 Submission Name : 1H-Benzotriazole

AR Number : 

Chemical Information

CAS Number : 95-14-7 Ninth Collective Index Name : 1H-Benzotriazole

Submitter Information

Submitter's Name : Orphan

Address : 

City : 

State : VA

ZIP/Postal Code: 

Country: 

Type of Submitter : Company

Technical Point of Contact Information

Technical Point of Contact : 

Title : 

Phone Number : 

Fax Number : 

E-Mail Address : 

Additional Technical Point of Contact Information

Technical Point of Contact : 

Title : 

Phone Number : 

Fax Number : 

E-Mail Address : 

Sponsor Information

Sponsor's Name : Orphan Chemical

Address : 

City : 

State : VA

ZIP/Postal Code : 

Country : US

Phone Number : 

Fax Number : 
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E-Mail Address : 

Type of Sponsor : Company

Technical Point of Contact Information

Technical Point of Contact : 

Title : 

Phone Number : 

Fax Number : 

E-Mail Address : 

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored

Chemical Name :

1H-Benzotriazole

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

purity not noted

Sponsored

Chemical Result

Type:

Test Substance

Result Type:

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

Method/Guideline

Followed:

EPA OTS 797.1400

Other

Method/Guideline:

Deviations from

Method/Guideline:

Species: Lepomis macrochirus

GLP: No

Analytical

Monitoring:

No Data

Test Type: Static

Test Vessel:

Water Media Type:

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours
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Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 25 mg/L
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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Results Remarks: Median Tolerance Limit (LC50) = 27.5 ppm (48 hrs); 25 ppm (96 hrs).

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: IUCLID (2001). Food and Drug Research Laboratories, Study No. 90801,

December 1, 1969.

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored

Chemical Name :

1H-Benzotriazole

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

1978

Method/Guideline

Followed:

Other

Other

Method/Guideline:

Tested in accordance with the method reported by Bathe et al. (1974) 

Deviations from

Method/Guideline:

Species: Oncorhynchus mykiss

GLP:

Analytical

Monitoring:

Test Type:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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Test Vessel: Closed

Water Media Type:

Test

Concentrations:

Nominal

Nominal and

Measured

Concentrations:

15, 21, 37 and 65 ppm

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Value/Lower Range: 12 Upper Range: 16 °C

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Method/guideline followed: Tested in accordance with the method reported by Bathe et

al. (1974) which is a modification of the procedure proposed by the United States

Department of the Interior Fish and Wildlife Service, reported in "Procedure for

Evaluation of Acute Toxicity of Pesticides to Fish and Wildlife" of December 14, 1964.

Prior to testing the food was withdrawn for 3 days. All fish were in good condition

and free from disease.

The fish were tested in glass tanks, of 12 and 15 litre capacity. Testing was

performed in reconstituted water prepared of deionised water to which salts were added

in the following amount per litre: 30 mg calcium sulphate, 30 mg magnesium sulphate,

48 mg sodium bicarbonate, 3 mg potassium chloride.

The fish were tested at 14 ± 2°C 

The water was not aerated during testing. Two to five fish were placed in each tank.

Various concentrations of TK 10637 were prepared by adding the test material diluted

in aqua dest. (stock solution) at the appropriate amount ensuring that the total

volume in each vessel remained the same.

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 23.9 ppm

Results Remarks: The test material has a slight acute toxicity to trout

Rainbow trout = 23.9 (17.6-29.9) ppm, LC 50 including 95% confidence limits were

calculated by the logit model. Concentrations were expressed as weight of substance

per volume of water, in parts per million (ppm). These values based upon nominal

concentrations of TK 10637.

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Acute toxicity to rainbow trout, carp, catfish and bluegill of TK 10, 637 with cover

letter dated 072893. Submitting Organization: Ciba-Geigy Corporation. OTS0538215.

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored

Chemical Name :

1H-Benzotriazole

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Purity not noted

Sponsored
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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Chemical Result

Type:

Test Substance

Result Type:

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

Species: Lebistes reticulatus

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: Flow-through

Test Vessel:

Water Media Type:

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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Remarks:

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % 28 mg/L

Results Remarks:

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability

Remarks:

Lacking number of fish tested, concentrations tested and study details.

(2 = valid with restrictions - per IUCLID Dataset.)

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: IUCLID (2001). Davis, L.N. et al.: Investigation of Selected Potential Environmental

Contaminants. Benzotriazoles. EPA 560/2-77-001, 137-138 (1977).

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored

Chemical Name :

1H-Benzotriazole
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Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

Method/Guideline

Followed:

Other

Method/Guideline:

Deviations from

Method/Guideline:

Species: Oncorhynchus mykiss

GLP:

Analytical

Monitoring:

Test Type: Static

Test Vessel:

Water Media Type: Freshwater

Test

Concentrations:

Nominal and

Measured

Concentrations:

5 ppm (5,000 μg/L)

Exposure Period: 24 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen: 8.6-13.7 ppm

pH Value: 7.5 Upper Range: 8.2

Test Temperature

and Units:

Value/Lower Range: 55 °F

Photo
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(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Rainbow Trout fingerlings, ≤ 4 inches

The test article was introduced at a concentration of 5,000 μg/L. The test

media was fresh water, temperature was kept at 55°F, and pH ranged from 7.5-8.2,

dissolved oxygen ranged from 8.6-13.7 ppm, 4 fish were used for testing.

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

Results Remarks: No effects were observed at 5 ppm.

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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Reference: ECOTOX (2010). 638 Applegate, V.C., J.H. Howell, A.E. Hall Jr., and M.A. Smith,

1957. Title: Toxicity of 4,346 Chemicals to Larval Lampreys and Fishes. Special

Scientific Report- -Fisheries No. 207. March 1957.

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored

Chemical Name :

1H-Benzotriazole

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

1978

Method/Guideline

Followed:

Other

Other

Method/Guideline:

Tested in accordance with the method reported by Bathe et al. (1974) 

Deviations from

Method/Guideline:

Species: Cyprinus carpio

GLP:

Analytical

Monitoring:

Test Type:

Test Vessel: Closed

Water Media Type:

Test

Concentrations:

Nominal

Nominal and

Measured

Concentrations:

8.7, 21, 37, 49 and 65 ppm

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:
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Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Value/Lower Range: 20 Upper Range: 22 °C

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Method/guideline followed: Tested in accordance with the method reported by Bathe et

al. (1974) which is a modification of the procedure proposed by the United States

Department of the Interior Fish and Wildlife Service, reported in "Procedure for

Evaluation of Acute Toxicity of Pesticides to Fish and Wildlife" of December 14, 1964.

Test method/conditions remarks: Prior to testing the food was withdrawn for 3

days. All fish were in good condition and free from disease.

The fish were tested in glass tanks, of 12 and 15 litre capacity. Testing was

performed in reconstituted water prepared of deionised water to which salts were added

in the following amount per litre: 30 mg calcium sulphate, 30 mg magnesium sulphate,

48 mg sodium bicarbonate, 3 mg potassium chloride.

The fish were tested at 14 ± 2°C (trout, bluegill) and at 21 ± 1°C (carp,

catfish). The water was not aerated during testing. Two to five fish were placed in

each tank.

Various concentrations of TK 10637 were prepared by adding the test material diluted

in aqua dest. (stock solution) at the appropriate amount ensuring that the total

volume in each vessel remained the same.

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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Value

96 Hours LC 50 % = 30 ppm

Results Remarks: The test material has a slight acute toxicity to tout and carp and is practically

devoid of acute toxicity to catfish and bluegill when tested and evaluated according

to the procedure of Bathe et al (1974).

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Acute toxicity to rainbow trout, carp, catfish and bluegill of TK 10, 637 with cover

letter dated 072893. Submitting Organization: Ciba-Geigy Corporation. OTS0538215.

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored

Chemical Name :

1H-Benzotriazole

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

Method/Guideline

Followed:

Other

Method/Guideline:

Deviations from

Method/Guideline:

Species: Oncorhynchus kisutch
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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GLP:

Analytical

Monitoring:

No

Test Type: Static

Test Vessel:

Water Media Type: Freshwater

Test

Concentrations:

Nominal and

Measured

Concentrations:

10 ppm (10,000 μg/L)

Exposure Period: 24 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity: 7 mg/L CaCO3

Dissolved Oxygen:

pH Value: Value/Lower Range: = 7.2

Test Temperature

and Units:

Value/Lower Range: 12 °C

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness: 7 mg/L

Method/Guideline

Test Conditions

Remarks:

The test article was introduced at a concentration of 10,000 ìg/L. The test media

was fresh water, temperature was kept at 12°C, and pH at 7.2, the water hardness

was 7 mg/L CaCO3, and the alkalinity was 7 mg/L CaCO3. Polyethylene bags were used

as the test vessel and aerated, 23 fish were used for testing.

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

Results Remarks: Neither loss of equilibrium nor death occurred.

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: ECOTOX (2010). 15148 MacPhee, C., and R. Ruelle, 1969. Lethal Effects of 1888

Chemicals Upon Four Species of Fish from Western North America. Bull. No. 3,

Forest, WildI. And Range Exp. Stn., Univ of Idaho, Moscow, ID: 112p.

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored

Chemical Name :

1H-Benzotriazole

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 10:27:28 AM]

Year Study

Performed:

1969

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

Species: Other

Other Species: Trout

GLP: No

Analytical

Monitoring:

Yes

Test Type: Flow-through

Test Vessel:

Water Media Type: Freshwater

Test

Concentrations:

Nominal and

Measured

Concentrations:

In order to estimate the concentration to be used in the dynamic system, a series of

static tests were performed using 0.1, 1.0, 10, 30, 50 and 100 ppm BT-D.

For dynamic test, concentrations were 10, 12, 14 and 16 ppm

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Trout were maintained for 96 hours prior to use at 55-60oF in specially designed

dynamic fresh water recirculating tanks of 100-gallon capacity, were fed a dry

commercial fish flake until 24 hours prior to initiation of testing.

Limit Test:
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Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

48 Hours LC 50 % 15 ppm Mortality Estimated

96 Hours LC 50 % 12 ppm Mortality Estimated

Results Remarks: TLm values were 15 and 12 ppm at 48 and 96 hours, respectively.

LC50 values were 15 and 12 ppm at 48 and 96 hours, respectively

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: The effects of BT-D on trout, bluegill and fathead minnow fingerlings with attachments

and cover letter dated 061289. Submitting Organization: Sherwin Williams

Company. Performing Laboratory: Food and Drug Research Labs. OTS0520640

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored

Chemical Name :

1H-Benzotriazole

Test Substance - Acute Toxicity To Aquatic Vertebrates
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 10:27:28 AM]

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

1969

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

Species: Lepomis macrochirus

GLP: No

Analytical

Monitoring:

Yes

Test Type: Static

Test Vessel:

Water Media Type: Freshwater

Test

Concentrations:

Nominal and

Measured

Concentrations:

22, 24, 26 and 28 ppm

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Bluegills were maintained in 100 gallon tanks for 96 hours at 68 oF, were fed a dry

commercial fish flake until 24 hours prior to initiation of testing.

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

48 Hours LC 50 % 27.5 ppm Mortality Estimated

96 Hours LC 50 % 25 ppm Mortality Estimated

Results Remarks: TLm values were 27.5 and 25.0 ppm at 48 and 96 hours, respectively.

LC50 values were 27.5 and 25.0 ppm at 48 and 96 hours, respectively.

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: The effects of BT-D on trout, bluegill and fathead minnow fingerlings with attachments

and cover letter dated 061289. Submitting Organization: Sherwin Williams

Company. Performing Laboratory: Food and Drug Research Labs. OTS0520640
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 10:27:28 AM]

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored

Chemical Name :

1H-Benzotriazole

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

1978

Method/Guideline

Followed:

Other

Other

Method/Guideline:

Tested in accordance with the method reported by Bathe et al. (1974) 

Deviations from

Method/Guideline:

Species: Lepomis macrochirus

GLP:

Analytical

Monitoring:

Test Type:

Test Vessel: Closed

Water Media Type:

Test

Concentrations:

Nominal

Nominal and

Measured

Concentrations:

10 and 100 ppm

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:
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Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Value/Lower Range: 20 Upper Range: 22 °C

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Method/guideline followed: Tested in accordance with the method reported by Bathe et

al. (1974) which is a modification of the procedure proposed by the United States

Department of the Interior Fish and Wildlife Service, reported in "Procedure for

Evaluation of Acute Toxicity of Pesticides to Fish and Wildlife" of December 14, 1964.

Test method/conditions remarks: Prior to testing the food was withdrawn for 3

days. All fish were in good condition and free from disease.

The fish were tested in glass tanks, of 12 and 15 litre capacity. Testing was

performed in reconstituted water prepared of deionised water to which salts were added

in the following amount per litre: 30 mg calcium sulphate, 30 mg magnesium sulphate,

48 mg sodium bicarbonate, 3 mg potassium chloride.

The fish were tested at 14 ± 2°C (trout, bluegill) and at 21 ± 1°C (carp,

catfish). The water was not aerated during testing. Two to five fish were placed in

each tank.

Various concentrations of TK 10637 were prepared by adding the test material diluted

in aqua dest. (stock solution) at the appropriate amount ensuring that the total

volume in each vessel remained the same.

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % > 100 ppm
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Results Remarks: The test material has a slight acute toxicity to tout and carp and is practically

devoid of acute toxicity to catfish and bluegill when tested and evaluated according

to the procedure of Bathe et al (1974).

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: Acute toxicity to rainbow trout, carp, catfish and bluegill of TK 10, 637 with cover

letter dated 072893. Submitting Organization: Ciba-Geigy Corporation. OTS0538215.

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored

Chemical Name :

1H-Benzotriazole

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

1969

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

Species: Pimephales promelas

GLP: No

Analytical

Monitoring:

Yes
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Test Type: Flow-through

Test Vessel:

Water Media Type: Freshwater

Test

Concentrations:

Nominal and

Measured

Concentrations:

26, 28, 30, 32 and 34 ppm

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Fathead minnows were maintained in 100 gallon tanks for 96 hours at 68 oF, were fed a

dry commercial fish flake until 24 hours prior to initiation of testing.

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

48 Hours LC 50 % 30.2 ppm Mortality Estimated

96 Hours LC 50 % 28 ppm Mortality Estimated

Results Remarks: TLm values were 30.2 and 28.0 ppm at 48 and 96 hours, respectively.

LC50 values were 30.2 and 28.0 ppm at 48 and 96 hours, respectively.

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: The effects of BT-D on trout, bluegill and fathead minnow fingerlings with attachments

and cover letter dated 061289. Submitting Organization: Sherwin Williams

Company. Performing Laboratory: Food and Drug Research Labs. OTS0520640

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored

Chemical Name :

1H-Benzotriazole

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

1969

Method/Guideline

Followed:

No Data
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Other

Method/Guideline:

Deviations from

Method/Guideline:

Species: Lepomis macrochirus

GLP: No

Analytical

Monitoring:

Yes

Test Type: Flow-through

Test Vessel:

Water Media Type: Freshwater

Test

Concentrations:

Nominal and

Measured

Concentrations:

26, 28, 30, 32 and 34 ppm

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Bluegills were maintained in 100 gallon tanks for 96 hours at 68 oF, were fed a dry

commercial fish flake until 24 hours prior to initiation of testing.

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

48 Hours LC 50 % 27.5 ppm Mortality Estimated

96 Hours LC 50 % 25 ppm Mortality Estimated

Results Remarks: TLm values were 27.5 and 25.0 ppm at 48 and 96 hours, respectively.

LC50 values were 27.5 and 25.0 ppm at 48 and 96 hours, respectively.

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: The effects of BT-D on trout, bluegill and fathead minnow fingerlings with attachments

and cover letter dated 061289. Submitting Organization: Sherwin Williams

Company. Performing Laboratory: Food and Drug Research Labs. OTS0520640

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored

Chemical Name :

1H-Benzotriazole

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

and Other Test

Substance

Comments:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

1969

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

Species: Pimephales promelas

GLP: No

Analytical

Monitoring:

Yes

Test Type: Static

Test Vessel:

Water Media Type: Freshwater

Test

Concentrations:

Nominal and

Measured

Concentrations:

22, 24, 26 and 28 ppm

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:
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Method/Guideline

Test Conditions

Remarks:

Fathead minnows were maintained in 100 gallon tanks for 96 hours at 68 oF, were fed a

dry commercial fish flake until 24 hours prior to initiation of testing.

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

48 Hours LC 50 % 30.2 ppm Mortality Estimated

96 Hours LC 50 % 28 ppm Mortality Estimated

Results Remarks: TLm values were 30.2 and 28.0 ppm at 48 and 96 hours, respectively.

LC50 values were 30.2 and 28.0 ppm at 48 and 96 hours, respectively.

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: The effects of BT-D on trout, bluegill and fathead minnow fingerlings with attachments

and cover letter dated 061289. Submitting Organization: Sherwin Williams

Company. Performing Laboratory: Food and Drug Research Labs. OTS0520640

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 10:27:28 AM]

Sponsored

Chemical Name :

1H-Benzotriazole

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Substance type: organic 

Physical status: solid 

Purity: ca. 100 % w/w

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

1984

Method/Guideline

Followed:

Other

Other

Method/Guideline:

Letale Wirkung beim Zebrabaerbling - Brachydanio rerio

Deviations from

Method/Guideline:

Species: Danio rerio

GLP: No Data

Analytical

Monitoring:

No

Test Type: Static

Test Vessel:

Water Media Type:

Test

Concentrations:

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value: 6.5 Upper Range: 7
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Test Temperature

and Units:

Value/Lower Range: 19.6 Upper Range: 23 °C

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

"Letale Wirkung beim Zebrabaerbling - Brachydanio rerio" (LC 0, LC 50, LC 100; 48-96

Stunden), Verfahrensvorschlag Umweltbundesamt, Mai 1984

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % > 100 mg/L

Results Remarks:

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability

Remarks:

4 = not assignable. Lacking number of fish tested, concentrations tested and study

details.

(2 = valid with restrictions Meets generally accepted scientific standards, well

documented and acceptable for assessment - per IUCLID Dataset.)
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Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: IUCLID (2001). Bayer report Prof. Caspers in 1985-08-03. 

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored

Chemical Name :

1H-Benzotriazole

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Cobratec 99; purity >99%

Sponsored

Chemical Result

Type:

Estimated by Calculation

Test Substance

Result Type:

Estimated

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

Method/Guideline

Followed:

Other

Other

Method/Guideline:

Batelle Protocol

Deviations from

Method/Guideline:

Species: Salmo sp.

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: No Data

Test Vessel:

Water Media Type:

Test

Concentrations:

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours

Vehicle Used:
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Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 39 mg/L

96 Hours EC 50 % = 24.4 mg/L
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Results Remarks: LC50 generated by Binomial method; 95% confidence limits = 28.4- 49.5 mg/L 

EC50 generated by Binomial method; 95% confidence limits = 21.0- 28.4 mg/L.

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability

Remarks:

Lacking number of fish tested, concentrations tested and study details.

(2 = valid with restrictions Meets generally accepted scientific standards, well

documented and acceptable for assessment - per IUCLID Dataset)

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: IUCLID (2001). Battelle Study. 10/1/85; PMC Specialties Group, Inc. MSDS.

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored

Chemical Name :

1H-Benzotriazole

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Purity not noted

Sponsored

Chemical Result

Type:

Test Substance

Result Type:

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

Species: Pimephales promelas

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: Flow-through

Test Vessel:
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Water Media Type:

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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Lower

Mean

Value

96 Hours LC 50 % 28 mg/L

Results Remarks:

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability

Remarks:

Lacking number of fish tested, concentrations tested and study details.

(2 = valid with restrictions - per IUCLID Dataset.)

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: IUCLID (2001). Davis, L.N. et al.: Investigation of Selected Potential Environmental

Contaminants. Benzotriazoles. EPA 560/2-77-001, 137-138 (1977).

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored

Chemical Name :

1H-Benzotriazole

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

purity not noted

Sponsored

Chemical Result

Type:

Test Substance

Result Type:

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

Species: Pimephales promelas
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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GLP: No Data

Analytical

Monitoring:

No Data

Test Type: Static

Test Vessel:

Water Media Type:

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 25 mg/L

Results Remarks:

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability

Remarks:

Lacking number of fish tested, concentrations tested and study details.

(2 = valid with restrictions - per IUCLID Dataset.)

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: IUCLID (2001). Davis, L.N. et al.: Investigation of Selected Potential Environmental

Contaminants. Benzotriazoles. EPA 560/2-77-001, 137-138 (1977).

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored

Chemical Name :

1H-Benzotriazole

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

purity not noted

Sponsored

Chemical Result

Type:

Test Substance

Result Type:

Method - Acute Toxicity To Aquatic Vertebrates
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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Year Study

Performed:

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

Species: Lepomis macrochirus

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: Static

Test Vessel:

Water Media Type:

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 10:27:28 AM]

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 25 mg/L

Results Remarks:

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability

Remarks:

Lacking number of fish tested, concentrations tested and study details.

(2 = valid with restrictions - per IUCLID Dataset.)

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: IUCLID (2001). Davis, L.N. et al.: Investigation of Selected Potential Environmental

Contaminants. Benzotriazoles. EPA 560/2-77-001, 137-138 (1977).

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored

Chemical Name :

1H-Benzotriazole

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

purity not noted
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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and Other Test

Substance

Comments:

Sponsored

Chemical Result

Type:

Test Substance

Result Type:

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

Species: Lepomis macrochirus

GLP: No

Analytical

Monitoring:

No Data

Test Type: Flow-through

Test Vessel:

Water Media Type:

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:
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Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 28 mg/L

Results Remarks: Median Tolerance Limit (LC50) = 30.2 ppm (48 hrs); 28.0 ppm (96 hrs).

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: IUCLID (2001). Food and Drug Research Laboratories, Study No. 90801,

December 1, 1969.

 Acute Toxicity to Aquatic Vertebrates
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CAS Number : 95-14-7

Sponsored

Chemical Name :

1H-Benzotriazole

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

purity not noted

Sponsored

Chemical Result

Type:

Test Substance

Result Type:

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

Method/Guideline

Followed:

No Data

Other

Method/Guideline:

Deviations from

Method/Guideline:

Species: Oncorhynchus mykiss (Fish, estuary, fresh water)

GLP: No

Analytical

Monitoring:

No Data

Test Type: Flow-through

Test Vessel:

Water Media Type:

Test

Concentrations:

No Data

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Test species: Rainbow trout, Salmo gairdneri (Fish, estuary, fresh water)

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 12 ppm

48 Hours LC 50 % = 15 ppm

Results Remarks: LC50 = 15 ppm (48 hrs); 12 ppm (96 hrs).

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability

Remarks:

Key Study Sponsor

Indicator:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 10:27:28 AM]

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: IUCLID (2001). Food and Drug Research Laboratories, Study No. 90801,

December 1, 1969.

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored

Chemical Name :

1H-Benzotriazole

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

purity not noted

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

1957

Method/Guideline

Followed:

Other

Method/Guideline:

Deviations from

Method/Guideline:

Species: Lepomis macrochirus

GLP:

Analytical

Monitoring:

No

Test Type: Static

Test Vessel:

Water Media Type:

Test

Concentrations:

Nominal and

Measured

Concentrations:

The test article was introduced at a one time concentration of 5000 μg/L.

Exposure Period: 24 Hours

Vehicle Used:

Vehicle Name:
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Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value: 7.5 Upper Range: 8.2

Test Temperature

and Units:

Value/Lower Range: 55 °F

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Test species: Bluegill sunfish (Lepomis macrochirus), 2 fish used, fingerlings,

≤ 4 inches

The test article was introduced at a one time concentration of 5000 μg/L. The

test media was fresh water, temperature was kept at 55°F, and pH ranged from 7.5-

8.2. 

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % > 5 ppm
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Results Remarks: No effects were observed at 5 ppm.

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: ECOTOX (2010). 638 Applegate, V.C., J.H. Howell, A.E. Hall Jr., and M.A. Smith, 1957.

Title: Toxicity of 4,346 Chemicals to Larval Lampreys and Fishes. Special Scientific

Report- -Fisheries No. 207. March 1957.

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored

Chemical Name :

1H-Benzotriazole

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

Sponsored

Chemical Result

Type:

Measured

Test Substance

Result Type:

Measured

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

Method/Guideline

Followed:

Other

Method/Guideline:

Deviations from

Method/Guideline:

Species: Oncorhynchus tschawytscha

GLP:

Analytical

Monitoring:

No

Test Type: Static

Test Vessel:
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Water Media Type: Freshwater

Test

Concentrations:

Nominal and

Measured

Concentrations:

10 ppm (10,000 μg/L)

Exposure Period: 24 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity: 7 mg/L CaCO3

Dissolved Oxygen:

pH Value: 7.2

Test Temperature

and Units:

Value/Lower Range: 12 °C

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness: 0 - 17 mg/L

Method/Guideline

Test Conditions

Remarks:

The test article was introduced at a concentration of 10,000 μg/L. The test

media was fresh water, temperature was kept at 12°C with a pH of 7.2, hardness of

0-17 mg/L CaCO3 and an alkalinity of 7 mg/L CaCO3. Polyethylene bags were used as

the test vessel, 22 fish were used for testing.

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure Exposure LC/EC % Value Mean Upper Units Effect Basis for
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 10:27:28 AM]

Duration Units Description Value

or

Lower

Mean

Value

Mean

Value

Observed Concentration

Results Remarks: Neither loss of equilibrium nor death occurred

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 2 - Valid With Restrictions

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: ECOTOX (2010). 15148 MacPhee, C., and R. Ruelle, 1969. Lethal Effects of 1888

Chemicals Upon Four Species of Fish from Western North America. Bull. No. 3,

Forest, WildI. And Range Exp. Stn., Univ of Idaho, Moscow, ID: 112p.

 Acute Toxicity to Aquatic Vertebrates

CAS Number : 95-14-7

Sponsored

Chemical Name :

1H-Benzotriazole

Test Substance - Acute Toxicity To Aquatic Vertebrates

Test Substance: (95-14-7) 1H-Benzotriazole

Test Substance

Purity/Composition

and Other Test

Substance

Comments:

purity not noted

Sponsored

Chemical Result

Type:

Test Substance

Result Type:

Method - Acute Toxicity To Aquatic Vertebrates

Year Study

Performed:

Method/Guideline

Followed:

Other

Method/Guideline:

Deviations from

Method/Guideline:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

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Species: Salmo sp.

GLP: No Data

Analytical

Monitoring:

No Data

Test Type: Flow-through

Test Vessel:

Water Media Type:

Test

Concentrations:

Nominal and

Measured

Concentrations:

Exposure Period: 96 Hours

Vehicle Used:

Vehicle Name:

Vehicle Amount

and Units:

Alkalinity:

Dissolved Oxygen:

pH Value:

Test Temperature

and Units:

Photo

(Light/Dark):

Salinity:

TOC:

Water Hardness:

Method/Guideline

Test Conditions

Remarks:

Test species: Rainbow trout, Salmo gairdneri (Fish, estuary, fresh water)

Fingerlings

Limit Test:

Test Results - Acute Toxicity To Aquatic Vertebrates

NOEC Exposure

Duration:

NOEC:

LOEC Exposure

Duration:

LOEC:
High Production Volume Information System (HPVIS) | High Production Volume (HPV) Challenge | OPPT | US EPA

http://iaspub.epa.gov/hpvis/ServSubmissionPrint[6/15/2011 10:27:28 AM]

NOELR Exposure

Duration:

NOELR:

LOELR Exposure

Duration:

LOELR:

LC/EC Mean Value : Exposure

Duration

Exposure

Units

LC/EC % Value

Description

Mean

Value

or

Lower

Mean

Value

Upper

Mean

Value

Units Effect

Observed

Basis for

Concentration

96 Hours LC 50 % = 12 mg/L

Results Remarks:

Reliability/Data Quality - Acute Toxicity To Aquatic Vertebrates

Reliability: 4 - Not Assignable

Reliability

Remarks:

Key Study Sponsor

Indicator:

Reference - Acute Toxicity To Aquatic Vertebrates

Reference: IUCLID (2001). Davis, L.N. et al.: Investigation of Selected Potential Environmental

Contaminants. Benzotriazoles. EPA 560/2-77-001, 137-138 (1977).