### 13339 Hagers Ferry Road Huntersville, NC 28078-7929 McGuire Nuclear Complex - MG03A2 Phone: 980-875-5245 Fax: 980-875-4349 

# Order Summary Report 

# Analytical Laboratory

Order Number: J12060052 Project Name: N/A

Lab Contact: Jason C Perkins 

### Date: 6/14/2012 Customer Address: 3195 Pine Hall Rd Customer Name(s): Bill K, Wayne C, Melonie M, Ron L., and Ted M.

Mailcode: Belews Steam Station Belews Creek, NC 28012

Phone: 980-875-5348 Report Authorized By: (Signature)


### Program Comments: Please contact the Program Manager (Jason C Perkins) with any questions regarding this report. Data Flags & Calculations: Any analytical tests or individual analytes within a test flagged with a Qualifier indicate a deviation from the method quality system or quality control requirement. The qualifier description is found at the end of the Certificate of Analysis (sample results) under the qualifiers heading. All results are reported on a dry weight basis unless otherwise noted. Data Package: This data package includes analytical results that are applicable only to the samples described in this narrative. An estimation of the uncertainty of measurement for the results in the report is available upon request. This report shall not be reproduced, except in full, without the written consent of the Analytical Laboratory. Please contact the Analytical laboratory with any questions. The order of individual sections within this report is as follows: Job Summary Report, Sample Identification, Technical Validation of Data Package, Analytical Laboratory Certificate of Analysis, Analytical Laboratory QC Reports, Sub-contracted Laboratory Results, Customer Specific Data Sheets, Reports & Documentation, Customer Database Entries, Test Case Narratives, Chain of Custody (COC) Certification: The Analytical Laboratory holds the following State Certifications : North Carolina (DENR) Certificate #248, South Carolina (DHEC) Laboratory ID # 99005. Contact the Analytical Laboratory for definitive information about the certification status of specific methods.

## Sample ID's & Descriptions:

Sample ID Plant/Station Collection Date and Time Collected By Sample Description 2012012224 BELEWS 01-Jun-12 7:30 AM W. B. WORKMAN FGD Purge Eff 2012012230 BELEWS 01-Jun-12 7:40 AM W. B. WORKMAN BIOREACTOR 1 INF 2012012231 BELEWS 01-Jun-12 W. B. WORKMAN biOREACTOR 1 INF HG BLK 2012012245 BELEWS 01-Jun-12 7:50 AM W. B. WORKMAN BIOREACTOR 2 EFF. 2012012246 BELEWS 01-Jun-12 W. B. WORKMAN BIOREACTOR 2 EFF. BLANK 2012012247 BELEWS 01-Jun-12 8:00 AM W. B. WORKMAN FILTER BLANK 2012012265 BELEWS 01-Jun-12 8:10 AM W. B. WORKMAN METALS TRIP BLANK 7 Total Samples

### COC and .pdf report are in agreement with sample totals

and analyses (compliance programs and procedures). All Results are less than the laboratory reporting limits. All laboratory QA/QC requirements are acceptable. The Vendor Laboratories have been qualified by the Analytical Laboratory Yes No



# Technical Validation Review Checklist:

Yes No

Yes No



## Report Sections Included:

Job Summary Report Sub-contracted Laboratory Results Sample Identification Customer Specific Data Sheets, Reports, & Documentation Technical Validation of Data Package Customer Database Entries Analytical Laboratory Certificate of Analysis Analytical Laboratory QC Report Chain of Custody

Reviewed By: 

### DataBase Administrator Date: 

### 6/14/2012 Yes

Electronic Data Deliverable (EDD) Sent Separately

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12060052

2012012224 Collection Date: 01-Jun-12 7:30 AM 

### Site: FGD Purge Eff Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF ALKALINITY Vendor Parameter Complete V_PRISM 1 NITRITE + NITRATE (COLORIMETRIC) Nitrite + Nitrate (Colorimetric) 14 mg-N/L EPA 353.2 04-Jun-12 11:37 TLINN 0.25 25 INORGANIC IONS BY IC Bromide 100 mg/L EPA 300.0 04-Jun-12 16:01 BGN9034 5 50 Chloride 7000 mg/L EPA 300.0 04-Jun-12 16:01 BGN9034 100 1000 Sulfate 1200 mg/L EPA 300.0 04-Jun-12 16:01 BGN9034 100 1000 MERCURY (COLD VAPOR) IN WATER Mercury (Hg) 237 ug/L EPA 245.1 07-Jun-12 11:43 AGIBBS 5 100 Mercury Dissolved (cold vapor) in Water (Filtered) Mercury (Hg) < 2.5 ug/L EPA 245.1 07-Jun-12 12:33 AGIBBS 2.5 50 DISSOLVED METALS BY ICP Manganese (Mn) 7.65 mg/L EPA 200.7 04-Jun-12 14:16 MHH7131 0.05 10 TOTAL RECOVERABLE METALS BY ICP Boron (B) 207 mg/L EPA 200.7 06-Jun-12 12:30 MHH7131 0.5 10 Calcium (Ca) 4770 mg/L EPA 200.7 06-Jun-12 12:30 MHH7131 0.1 10 Iron (Fe) 109 mg/L EPA 200.7 06-Jun-12 12:30 MHH7131 0.1 10 Lithium (Li) 0.170 mg/L EPA 200.7 06-Jun-12 12:30 MHH7131 0.05 10 Magnesium (Mg) 790 mg/L EPA 200.7 06-Jun-12 12:30 MHH7131 0.05 10 Manganese (Mn) 7.66 mg/L EPA 200.7 06-Jun-12 12:30 MHH7131 0.05 10 Potassium (K) 65.3 mg/L EPA 200.7 06-Jun-12 12:30 MHH7131 1 10 Sodium (Na) 47.6 mg/L EPA 200.7 06-Jun-12 12:30 MHH7131 0.5 10 DISSOLVED METALS BY ICP-MS Selenium (Se) 179 ug/L EPA 200.8 06-Jun-12 13:15 DJSULL1 10 10 TOTAL RECOVERABLE METALS BY ICP-MS Arsenic (As) 182 ug/L EPA 200.8 05-Jun-12 14:04 DJSULL1 10 10 Cadmium (Cd) < 10 ug/L EPA 200.8 05-Jun-12 14:04 DJSULL1 10 10 Chromium (Cr) 242 ug/L EPA 200.8 05-Jun-12 14:04 DJSULL1 10 10 Copper (Cu) 122 ug/L EPA 200.8 05-Jun-12 14:04 DJSULL1 10 10 Nickel (Ni) 201 ug/L EPA 200.8 05-Jun-12 14:04 DJSULL1 10 10 Selenium (Se) 4180 ug/L EPA 200.8 05-Jun-12 14:04 DJSULL1 10 10 Silver (Ag) < 10 ug/L EPA 200.8 05-Jun-12 14:04 DJSULL1 10 10 Zinc (Zn) 257 ug/L EPA 200.8 05-Jun-12 14:04 DJSULL1 10 10

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12060052

2012012224 Collection Date: 01-Jun-12 7:30 AM 

### Site: FGD Purge Eff Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF SELENIUM SPECIATION Vendor Parameter Complete V_AS&C 1 TOTAL DISSOLVED SOLIDS Vendor Parameter Complete V_PACE 1 TOTAL SUSPENDED SOLIDS Vendor Parameter Complete V_PACE 1


### 2012012230 Collection Date: 01-Jun-12 7:40 AM 

### Site: BIOREACTOR 1 INF Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF ALKALINITY Vendor Parameter Complete V_PRISM 1 NITRITE + NITRATE (COLORIMETRIC) Nitrite + Nitrate (Colorimetric) 15 mg-N/L EPA 353.2 04-Jun-12 11:40 TLINN 0.25 25 INORGANIC IONS BY IC Bromide 96 mg/L EPA 300.0 04-Jun-12 16:19 BGN9034 5 50 Chloride 6900 mg/L EPA 300.0 04-Jun-12 16:19 BGN9034 100 1000 Sulfate 1300 mg/L EPA 300.0 04-Jun-12 16:19 BGN9034 100 1000 MERCURY 1631 Vendor Parameter Complete V_BRAND 1 MERCURY (COLD VAPOR) IN WATER Mercury (Hg) < 2.5 ug/L EPA 245.1 07-Jun-12 11:45 AGIBBS 2.5 50 Mercury Dissolved (cold vapor) in Water (Filtered) Mercury (Hg) < 2.5 ug/L EPA 245.1 07-Jun-12 12:35 AGIBBS 2.5 50 DISSOLVED METALS BY ICP Manganese (Mn) 3.58 mg/L EPA 200.7 04-Jun-12 14:20 MHH7131 0.05 10

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12060052

2012012230 Collection Date: 01-Jun-12 7:40 AM 

### Site: BIOREACTOR 1 INF Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF TOTAL RECOVERABLE METALS BY ICP Boron (B) 203 mg/L EPA 200.7 06-Jun-12 12:34 MHH7131 0.5 10 Calcium (Ca) 3390 mg/L EPA 200.7 06-Jun-12 12:34 MHH7131 0.1 10 Iron (Fe) < 0.1 mg/L EPA 200.7 06-Jun-12 12:34 MHH7131 0.1 10 Lithium (Li) < 0.05 mg/L EPA 200.7 06-Jun-12 12:34 MHH7131 0.05 10 Magnesium (Mg) 728 mg/L EPA 200.7 06-Jun-12 12:34 MHH7131 0.05 10 Manganese (Mn) 3.52 mg/L EPA 200.7 06-Jun-12 12:34 MHH7131 0.05 10 Potassium (K) 25.1 mg/L EPA 200.7 06-Jun-12 12:34 MHH7131 1 10 Sodium (Na) 46.1 mg/L EPA 200.7 06-Jun-12 12:34 MHH7131 0.5 10 DISSOLVED METALS BY ICP-MS Selenium (Se) 92.6 ug/L EPA 200.8 06-Jun-12 13:19 DJSULL1 5 5 TOTAL RECOVERABLE METALS BY ICP-MS Arsenic (As) < 10 ug/L EPA 200.8 05-Jun-12 14:07 DJSULL1 10 10 Cadmium (Cd) < 10 ug/L EPA 200.8 05-Jun-12 14:07 DJSULL1 10 10 Chromium (Cr) < 10 ug/L EPA 200.8 05-Jun-12 14:07 DJSULL1 10 10 Copper (Cu) < 10 ug/L EPA 200.8 05-Jun-12 14:07 DJSULL1 10 10 Nickel (Ni) 25.9 ug/L EPA 200.8 05-Jun-12 14:07 DJSULL1 10 10 Selenium (Se) 113 ug/L EPA 200.8 05-Jun-12 14:07 DJSULL1 10 10 Silver (Ag) < 10 ug/L EPA 200.8 05-Jun-12 14:07 DJSULL1 10 10 Zinc (Zn) < 10 ug/L EPA 200.8 05-Jun-12 14:07 DJSULL1 10 10 SELENIUM SPECIATION Vendor Parameter Complete V_AS&C 1


### 2012012231 Collection Date: 01-Jun-12 

### Site: biOREACTOR 1 INF HG BLK Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF MERCURY 1631 Vendor Parameter Complete V_BRAND 1


### 2012012245 Collection Date: 01-Jun-12 7:50 AM 

### Site: BIOREACTOR 2 EFF. Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF ALKALINITY Vendor Parameter Complete V_PRISM 1

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12060052

2012012245 Collection Date: 01-Jun-12 7:50 AM 

### Site: BIOREACTOR 2 EFF. Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF NITRITE + NITRATE (COLORIMETRIC) Nitrite + Nitrate (Colorimetric) < 0.01 mg-N/L EPA 353.2 04-Jun-12 11:41 TLINN 0.01 1 INORGANIC IONS BY IC Bromide 99 mg/L EPA 300.0 04-Jun-12 16:37 BGN9034 5 50 Chloride 7000 mg/L EPA 300.0 04-Jun-12 16:37 BGN9034 100 1000 Sulfate 1300 mg/L EPA 300.0 04-Jun-12 16:37 BGN9034 100 1000 MERCURY 1631 Vendor Parameter Complete V_BRAND 1 MERCURY (COLD VAPOR) IN WATER Mercury (Hg) < 1 ug/L EPA 245.1 07-Jun-12 11:48 AGIBBS 1 20 Mercury Dissolved (cold vapor) in Water (Filtered) Mercury (Hg) < 2.5 ug/L EPA 245.1 07-Jun-12 12:37 AGIBBS 2.5 50 DISSOLVED METALS BY ICP Manganese (Mn) 4.49 mg/L EPA 200.7 04-Jun-12 14:24 MHH7131 0.05 10 TOTAL RECOVERABLE METALS BY ICP Boron (B) 210 mg/L EPA 200.7 06-Jun-12 12:38 MHH7131 0.5 10 Calcium (Ca) 3440 mg/L EPA 200.7 06-Jun-12 12:38 MHH7131 0.1 10 Iron (Fe) < 0.1 mg/L EPA 200.7 06-Jun-12 12:38 MHH7131 0.1 10 Lithium (Li) < 0.05 mg/L EPA 200.7 06-Jun-12 12:38 MHH7131 0.05 10 Magnesium (Mg) 764 mg/L EPA 200.7 06-Jun-12 12:38 MHH7131 0.05 10 Manganese (Mn) 4.38 mg/L EPA 200.7 06-Jun-12 12:38 MHH7131 0.05 10 Potassium (K) 30.1 mg/L EPA 200.7 06-Jun-12 12:38 MHH7131 1 10 Sodium (Na) 46.3 mg/L EPA 200.7 06-Jun-12 12:38 MHH7131 0.5 10 DISSOLVED METALS BY ICP-MS Selenium (Se) 5.40 ug/L EPA 200.8 06-Jun-12 14:14 DJSULL1 5 5 TOTAL RECOVERABLE METALS BY ICP-MS Arsenic (As) < 10 ug/L EPA 200.8 05-Jun-12 14:10 DJSULL1 10 10 Cadmium (Cd) < 10 ug/L EPA 200.8 05-Jun-12 14:10 DJSULL1 10 10 Chromium (Cr) < 10 ug/L EPA 200.8 05-Jun-12 14:10 DJSULL1 10 10 Copper (Cu) < 10 ug/L EPA 200.8 05-Jun-12 14:10 DJSULL1 10 10 Nickel (Ni) < 10 ug/L EPA 200.8 05-Jun-12 14:10 DJSULL1 10 10 Selenium (Se) 12.9 ug/L EPA 200.8 05-Jun-12 14:10 DJSULL1 10 10 Silver (Ag) < 10 ug/L EPA 200.8 05-Jun-12 14:10 DJSULL1 10 10 Zinc (Zn) < 10 ug/L EPA 200.8 05-Jun-12 14:10 DJSULL1 10 10 SELENIUM SPECIATION Vendor Parameter Complete V_AS&C 1

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12060052

2012012245 Collection Date: 01-Jun-12 7:50 AM 

### Site: BIOREACTOR 2 EFF. Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF


### 2012012246 Collection Date: 01-Jun-12 

### Site: BIOREACTOR 2 EFF. BLANK Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF MERCURY 1631 Vendor Parameter Complete V_BRAND 1


### 2012012247 Collection Date: 01-Jun-12 8:00 AM 

### Site: FILTER BLANK Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF Mercury Dissolved (cold vapor) in Water (Filtered) Mercury (Hg) < 0.05 ug/L EPA 245.1 07-Jun-12 12:40 AGIBBS 0.05 1 DISSOLVED METALS BY ICP Manganese (Mn) 0.018 mg/L EPA 200.7 04-Jun-12 14:04 MHH7131 0.005 1 DISSOLVED METALS BY ICP-MS Selenium (Se) < 1 ug/L EPA 200.8 06-Jun-12 13:03 DJSULL1 1 1


### 2012012265 Collection Date: 01-Jun-12 8:10 AM 

### Site: METALS TRIP BLANK Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF TOTAL RECOVERABLE METALS BY ICP Boron (B) < 0.05 mg/L EPA 200.7 06-Jun-12 11:59 MHH7131 0.05 1 Calcium (Ca) 0.685 mg/L EPA 200.7 06-Jun-12 11:59 MHH7131 0.01 1 Iron (Fe) < 0.01 mg/L EPA 200.7 06-Jun-12 11:59 MHH7131 0.01 1 Lithium (Li) < 0.005 mg/L EPA 200.7 06-Jun-12 11:59 MHH7131 0.005 1 Magnesium (Mg) 0.090 mg/L EPA 200.7 06-Jun-12 11:59 MHH7131 0.005 1 Manganese (Mn) < 0.005 mg/L EPA 200.7 06-Jun-12 11:59 MHH7131 0.005 1 Potassium (K) < 0.1 mg/L EPA 200.7 06-Jun-12 11:59 MHH7131 0.1 1 Sodium (Na) < 0.05 mg/L EPA 200.7 06-Jun-12 11:59 MHH7131 0.05 1

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12060052

2012012265 Collection Date: 01-Jun-12 8:10 AM 

### Site: METALS TRIP BLANK Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF TOTAL RECOVERABLE METALS BY ICP-MS Arsenic (As) < 1 ug/L EPA 200.8 05-Jun-12 13:51 DJSULL1 1 1 Cadmium (Cd) < 1 ug/L EPA 200.8 05-Jun-12 13:51 DJSULL1 1 1 Chromium (Cr) < 1 ug/L EPA 200.8 05-Jun-12 13:51 DJSULL1 1 1 Copper (Cu) 2.06 ug/L EPA 200.8 05-Jun-12 13:51 DJSULL1 1 1 Nickel (Ni) < 1 ug/L EPA 200.8 05-Jun-12 13:51 DJSULL1 1 1 Selenium (Se) 1.48 ug/L EPA 200.8 05-Jun-12 13:51 DJSULL1 1 1 Silver (Ag) < 1 ug/L EPA 200.8 05-Jun-12 13:51 DJSULL1 1 1 Zinc (Zn) < 1 ug/L EPA 200.8 05-Jun-12 13:51 DJSULL1 1 1 SELENIUM SPECIATION Vendor Parameter Complete V_AS&C 1

### This data package contains the analytical results for the project identified above and includes a Case Narrative, Sample Results and Chain of Custody. Unless otherwise noted, all samples were received in acceptable condition and processed according to the referenced methods. Data qualifiers are flagged individually on each sample. A key reference for the data qualifiers appears at the end of this case narrative. NC Certification No. 402 SC Certification No. 99012 NC Drinking Water Cert No. 37735 

# Case Narrative 06/08/2012

Duke Energy Corporation (04) Jay Perkins 13339 Hagers Ferry Road Huntersville, NC 28078 Project: HAPS/MACT Testing Belews Creek Project No.: J12060052 Lab Submittal Date: 06/01/2012 Prism Work Order: 2060015 VA Certification No. 1287



### Please call if you have any questions relating to this analytical report. Respectfully, PRISM LABORATORIES, INC.



### VP Laboratory Services Reviewed By

Data Qualifiers Key Reference: 

### HT Sample received and analyzed outside of the hold time.

Method Detection Limit MDL Below Reporting Limit BRL
Relative Percent Difference RPD


## * Results reported to the reporting limit. All other results are reported to the MDL with values between MDL and reporting limit indicated with a J.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 1 of 8

# Sample Receipt Summary 06/08/2012 

## Prism Work Order: 2060015 Client Sample ID Matrix Lab Sample ID Date Sampled Date Received 

### 2060015-01 06/01/12 2012012224/FGD Purge Eff 06/01/12 Water 2060015-02 06/01/12 2012012230/BioReactor 1 Inf 06/01/12 Water 2060015-03 06/01/12 2012012245/BioReactor 2 Eff 06/01/12 Water Samples received in good condition at 3.2 degrees C unless otherwise noted.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 2 of 8

# Laboratory Report 06/08/2012

Result Parameter Batch ID Report Limit Analysis Date/Time Method Dilution Factor Units MDL Analyst 

### 13339 Hagers Ferry Road Project No.: Project: HAPS/MACT Testing Belews Creek Duke Energy Corporation (04)

Huntersville, NC 28078 Attn: Jay Perkins Sample Matrix: Water Client Sample ID: Prism Sample ID: 2060015-01 Prism Work Order: 2060015 Time Collected: 06/01/12 07:30 Time Submitted: 06/01/12 15:35 2012012224/FGD Purge Eff


### J12060052

General Chemistry Parameters 7.0 *SM4500-H B P2F0036 1 6/4/12 13:00 JAB HT pH Units pH 76 *SM2320 B P2F0028 1 5.0 6/4/12 10:30 JAB mg/L 0.66 Total Alkalinity BRL *SM2320 B P2F0029 1 5.0 6/4/12 10:30 JAB mg/L 0.66 Carbonate Alkalinity 76 *SM2320 B P2F0030 1 5.0 6/4/12 10:30 JAB mg/L 0.66 Bicarbonate Alkalinity

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc. 449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 3 of 8

# Laboratory Report 06/08/2012

Result Parameter Batch ID Report Limit Analysis Date/Time Method Dilution Factor Units MDL Analyst 

### 13339 Hagers Ferry Road Project No.: Project: HAPS/MACT Testing Belews Creek Duke Energy Corporation (04)

Huntersville, NC 28078 Attn: Jay Perkins Sample Matrix: Water Client Sample ID: Prism Sample ID: 2060015-02 Prism Work Order: 2060015 Time Collected: 06/01/12 07:40 Time Submitted: 06/01/12 15:35 2012012230/BioReactor 1 Inf


### J12060052

General Chemistry Parameters 7.0 *SM4500-H B P2F0036 1 6/4/12 13:00 JAB HT pH Units pH 44 *SM2320 B P2F0028 1 5.0 6/4/12 10:30 JAB mg/L 0.66 Total Alkalinity BRL *SM2320 B P2F0029 1 5.0 6/4/12 10:30 JAB mg/L 0.66 Carbonate Alkalinity 44 *SM2320 B P2F0030 1 5.0 6/4/12 10:30 JAB mg/L 0.66 Bicarbonate Alkalinity

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc. 449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 4 of 8

# Laboratory Report 06/08/2012

Result Parameter Batch ID Report Limit Analysis Date/Time Method Dilution Factor Units MDL Analyst 

### 13339 Hagers Ferry Road Project No.: Project: HAPS/MACT Testing Belews Creek Duke Energy Corporation (04)

Huntersville, NC 28078 Attn: Jay Perkins Sample Matrix: Water Client Sample ID: Prism Sample ID: 2060015-03 Prism Work Order: 2060015 Time Collected: 06/01/12 07:50 Time Submitted: 06/01/12 15:35 2012012245/BioReactor 2 Eff


### J12060052

General Chemistry Parameters 6.9 *SM4500-H B P2F0036 1 6/4/12 13:00 JAB HT pH Units pH 110 *SM2320 B P2F0028 1 5.0 6/4/12 10:30 JAB mg/L 0.66 Total Alkalinity BRL *SM2320 B P2F0029 1 5.0 6/4/12 10:30 JAB mg/L 0.66 Carbonate Alkalinity 110 *SM2320 B P2F0030 1 5.0 6/4/12 10:30 JAB mg/L 0.66 Bicarbonate Alkalinity

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc. 449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 5 of 8

### 6/8/12 

## Level II QC Report

13339 Hagers Ferry Road Project No: J12060052 Project: HAPS/MACT Testing Belews Creek Duke Energy Corporation (04)

Huntersville, NC 28078 Attn: Jay Perkins Prism Work Order: 2060015 Time Submitted: 6/1/2012 3:35:00PM

Result Limit

Reporting Units Level Spike Result

Source %REC %REC Limits RPD RPD Limit Notes Analyte General Chemistry Parameters - Quality Control



### Batch P2F0028 - NO PREP Blank (P2F0028-BLK1) 

### Prepared & Analyzed: 06/04/12 Total Alkalinity mg/L BRL 5.0 LCS (P2F0028-BS1) 

### Prepared & Analyzed: 06/04/12 Total Alkalinity mg/L 253 5.0 250.0 90-110 101 LCS Dup (P2F0028-BSD1) 

### Prepared & Analyzed: 06/04/12 Total Alkalinity mg/L 250 5.0 250.0 200 90-110 100 1 

### Batch P2F0029 - NO PREP Blank (P2F0029-BLK1) 

### Prepared & Analyzed: 06/04/12 Carbonate Alkalinity mg/L BRL 5.0 LCS (P2F0029-BS1) 

### Prepared & Analyzed: 06/04/12 Carbonate Alkalinity mg/L 253 5.0 90-110 LCS Dup (P2F0029-BSD1) 

### Prepared & Analyzed: 06/04/12 Carbonate Alkalinity mg/L 250 5.0 200 90-110 1 

### Batch P2F0030 - NO PREP Blank (P2F0030-BLK1) 

### Prepared & Analyzed: 06/04/12 Bicarbonate Alkalinity mg/L BRL 5.0 LCS (P2F0030-BS1) 

### Prepared & Analyzed: 06/04/12 Bicarbonate Alkalinity mg/L 253 5.0 250.0 90-110 101

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 6 of 8

### 6/8/12 

## Level II QC Report

13339 Hagers Ferry Road Project No: J12060052 Project: HAPS/MACT Testing Belews Creek Duke Energy Corporation (04)

Huntersville, NC 28078 Attn: Jay Perkins Prism Work Order: 2060015 Time Submitted: 6/1/2012 3:35:00PM

Result Limit

Reporting Units Level Spike Result

Source %REC %REC Limits RPD RPD Limit Notes Analyte General Chemistry Parameters - Quality Control



### Batch P2F0030 - NO PREP LCS Dup (P2F0030-BSD1) 

### Prepared & Analyzed: 06/04/12 Bicarbonate Alkalinity mg/L 250 5.0 250.0 200 90-110 100 1 

### Batch P2F0036 - NO PREP LCS (P2F0036-BS1) 

### Prepared & Analyzed: 06/04/12 pH pH Units 6.81 6.860 99-101 99 Duplicate (P2F0036-DUP1) 

### Prepared & Analyzed: 06/04/12 Source: 2060015-01 pH pH Units 7.03 7.03 10 0

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 7 of 8

### Page 8 of 8

## June 06, 2012

LIMS USE: FR - PROGRAM MANAGER LIMS OBJECT ID: 92119831



## 92119831 Project: Pace Project No.: RE: Program Manager Duke Energy

,

J12060052

Dear Program Manager: Enclosed are the analytical results for sample(s) received by the laboratory on June 01, 2012. The results relate only to the samples included in this report. Results reported herein conform to the most current TNI standards and the laboratory's Quality Assurance Manual, where applicable, unless otherwise noted in the body of the report.
Analyses were performed at the Pace Analytical Services location indicated on the sample analyte page for analysis unless otherwise footnoted. If you have any questions concerning this report, please feel free to contact me.
Sincerely,

Kevin Herring kevin.herring@pacelabs.com Project Manager

Enclosures



### REPORT OF LABORATORY ANALYSIS This report shall not be reproduced, except in full, without the written consent of Pace Analytical Services, Inc.. Page 1 of 9 Pace Analytical Services, Inc. 9800 Kincey Ave. Suite 100 Huntersville, NC 28078 (704)875-9092 Pace Analytical Services, Inc.

2225 Riverside Dr. Asheville, NC 28804 (828)254-7176 Pace Analytical Services, Inc. 205 East Meadow Road - Suite A

Eden, NC 27288 (336)623-8921

### CERTIFICATIONS

Pace Project No.: Project: 92119831

J12060052

Asheville Certification IDs 2225 Riverside Dr., Asheville, NC 28804 Florida/NELAP Certification #: E87648 Massachusetts Certification #: M-NC030 North Carolina Drinking Water Certification #: 37712 North Carolina Wastewater Certification #: 40 South Carolina Certification #: 99030001 Virginia Certification #: 00072 West Virginia Certification #: 356 Virgina/VELAP Certification #: 460147



### REPORT OF LABORATORY ANALYSIS This report shall not be reproduced, except in full, without the written consent of Pace Analytical Services, Inc.. Page 2 of 9 Pace Analytical Services, Inc. 9800 Kincey Ave. Suite 100 Huntersville, NC 28078 (704)875-9092 Pace Analytical Services, Inc.

2225 Riverside Dr. Asheville, NC 28804 (828)254-7176 Pace Analytical Services, Inc. 205 East Meadow Road - Suite A

Eden, NC 27288 (336)623-8921

### SAMPLE SUMMARY

Pace Project No.: Project: 92119831

J12060052

Lab ID Sample ID Matrix Date Collected Date Received 92119831001 2012012224 Water 06/01/12 07:30 06/01/12 15:25



### REPORT OF LABORATORY ANALYSIS This report shall not be reproduced, except in full, without the written consent of Pace Analytical Services, Inc.. Page 3 of 9 Pace Analytical Services, Inc. 9800 Kincey Ave. Suite 100 Huntersville, NC 28078 (704)875-9092 Pace Analytical Services, Inc.

2225 Riverside Dr. Asheville, NC 28804 (828)254-7176 Pace Analytical Services, Inc. 205 East Meadow Road - Suite A

Eden, NC 27288 (336)623-8921

### SAMPLE ANALYTE COUNT

Pace Project No.: Project: 92119831

J12060052

Lab ID Sample ID Method Analytes Reported Laboratory Analysts 92119831001 2012012224 SM 2540C 1 PASI-A LMD SM 2540D 1 PASI-A LMD



### REPORT OF LABORATORY ANALYSIS This report shall not be reproduced, except in full, without the written consent of Pace Analytical Services, Inc.. Page 4 of 9 Pace Analytical Services, Inc. 9800 Kincey Ave. Suite 100 Huntersville, NC 28078 (704)875-9092 Pace Analytical Services, Inc.

2225 Riverside Dr. Asheville, NC 28804 (828)254-7176 Pace Analytical Services, Inc. 205 East Meadow Road - Suite A

Eden, NC 27288 (336)623-8921

### ANALYTICAL RESULTS

Pace Project No.: Project: 92119831

J12060052
Sample: 2012012224 Lab ID: 92119831001 Collected: 06/01/12 07:30 Received: 06/01/12 15:25 Matrix: Water

Parameters Results Units DF Prepared Analyzed CAS No. Qual MDL Limit Report

2540C Total Dissolved Solids Analytical Method: SM 2540C Total Dissolved Solids 16600 mg/L 1 06/04/12 19:31 500 500 2540D Total Suspended Solids Analytical Method: SM 2540D Total Suspended Solids 6460 mg/L 1 06/05/12 18:06 250 250



### REPORT OF LABORATORY ANALYSIS This report shall not be reproduced, except in full, without the written consent of Pace Analytical Services, Inc.. Date: 06/06/2012 03:52 PM Page 5 of 9 Pace Analytical Services, Inc. 9800 Kincey Ave. Suite 100 Huntersville, NC 28078 (704)875-9092 Pace Analytical Services, Inc.

2225 Riverside Dr. Asheville, NC 28804 (828)254-7176 Pace Analytical Services, Inc. 205 East Meadow Road - Suite A

Eden, NC 27288 (336)623-8921

### QUALITY CONTROL DATA

Pace Project No.: Project: 92119831

J12060052

QC Batch: QC Batch Method: Analysis Method: Analysis Description: WET/21085 SM 2540C SM 2540C 2540C Total Dissolved Solids Associated Lab Samples: 92119831001

Parameter Units Blank Result Reporting

Limit Qualifiers METHOD BLANK: 772818 Associated Lab Samples: 92119831001 Matrix: Water

Analyzed Total Dissolved Solids mg/L ND 25.0 06/04/12 19:29

Parameter Units LCS Result % Rec Limits Qualifiers % Rec Conc. 772819 LABORATORY CONTROL SAMPLE: LCS Spike

Total Dissolved Solids mg/L 252 250 101 80-120

Parameter Units Dup Result Max RPD Qualifiers RPD Result 92119843001 772821 SAMPLE DUPLICATE:

Total Dissolved Solids mg/L 14600 0 10 14600



### REPORT OF LABORATORY ANALYSIS This report shall not be reproduced, except in full, without the written consent of Pace Analytical Services, Inc.. Date: 06/06/2012 03:52 PM Page 6 of 9 Pace Analytical Services, Inc. 9800 Kincey Ave. Suite 100 Huntersville, NC 28078 (704)875-9092 Pace Analytical Services, Inc.

2225 Riverside Dr. Asheville, NC 28804 (828)254-7176 Pace Analytical Services, Inc. 205 East Meadow Road - Suite A

Eden, NC 27288 (336)623-8921

### QUALITY CONTROL DATA

Pace Project No.: Project: 92119831

J12060052

QC Batch: QC Batch Method: Analysis Method: Analysis Description: WET/21099 SM 2540D SM 2540D 2540D Total Suspended Solids Associated Lab Samples: 92119831001

Parameter Units Blank Result Reporting

Limit Qualifiers METHOD BLANK: 773425 Associated Lab Samples: 92119831001 Matrix: Water

Analyzed Total Suspended Solids mg/L ND 2.5 06/05/12 18:05

Parameter Units LCS Result % Rec Limits Qualifiers % Rec Conc. 773426 LABORATORY CONTROL SAMPLE: LCS Spike Total Suspended Solids mg/L 256 250 102 80-120

Parameter Units Dup Result Max RPD Qualifiers RPD Result 92119831001 773427 SAMPLE DUPLICATE:

Total Suspended Solids mg/L 6090 6 10 6460

Parameter Units Dup Result Max RPD Qualifiers RPD Result 92119673001 773428 SAMPLE DUPLICATE:

Total Suspended Solids mg/L 13.4 0 10 13.4



### REPORT OF LABORATORY ANALYSIS This report shall not be reproduced, except in full, without the written consent of Pace Analytical Services, Inc.. Date: 06/06/2012 03:52 PM Page 7 of 9 Pace Analytical Services, Inc. 9800 Kincey Ave. Suite 100 Huntersville, NC 28078 (704)875-9092 Pace Analytical Services, Inc.

2225 Riverside Dr. Asheville, NC 28804 (828)254-7176 Pace Analytical Services, Inc. 205 East Meadow Road - Suite A

Eden, NC 27288 (336)623-8921

### QUALIFIERS

Pace Project No.: Project: 92119831

J12060052

DEFINITIONS DF - Dilution Factor, if reported, represents the factor applied to the reported data due to changes in sample preparation, dilution of the sample aliquot, or moisture content. ND - Not Detected at or above adjusted reporting limit. J - Estimated concentration above the adjusted method detection limit and below the adjusted reporting limit. MDL - Adjusted Method Detection Limit. PRL - Pace Reporting Limit. RL - Reporting Limit. S - Surrogate 1,2-Diphenylhydrazine (8270 listed analyte) decomposes to Azobenzene. Consistent with EPA guidelines, unrounded data are displayed and have been used to calculate % recovery and RPD values. LCS(D) - Laboratory Control Sample (Duplicate) MS(D) - Matrix Spike (Duplicate) DUP - Sample Duplicate RPD - Relative Percent Difference NC - Not Calculable. SG - Silica Gel - Clean-Up U - Indicates the compound was analyzed for, but not detected. N-Nitrosodiphenylamine decomposes and cannot be separated from Diphenylamine using Method 8270. The result reported for each analyte is a combined concentration. Acid preservation may not be appropriate for 2-Chloroethylvinyl ether, Styrene, and Vinyl chloride. Pace Analytical is TNI accredited. Contact your Pace PM for the current list of accredited analytes. TNI - The NELAC Institute. LABORATORIES Pace Analytical Services - Asheville PASI-A



### REPORT OF LABORATORY ANALYSIS This report shall not be reproduced, except in full, without the written consent of Pace Analytical Services, Inc.. Date: 06/06/2012 03:52 PM Page 8 of 9 Pace Analytical Services, Inc. 9800 Kincey Ave. Suite 100 Huntersville, NC 28078 (704)875-9092 Pace Analytical Services, Inc.

2225 Riverside Dr. Asheville, NC 28804 (828)254-7176 Pace Analytical Services, Inc. 205 East Meadow Road - Suite A

Eden, NC 27288 (336)623-8921

### QUALITY CONTROL DATA CROSS REFERENCE TABLE

Pace Project No.: Project: 92119831

J12060052

Lab ID Sample ID QC Batch Method QC Batch Analytical Method Analytical Batch 92119831001 WET/21085 2012012224 SM 2540C 92119831001 WET/21099 2012012224 SM 2540D



### REPORT OF LABORATORY ANALYSIS This report shall not be reproduced, except in full, without the written consent of Pace Analytical Services, Inc.. Date: 06/06/2012 03:52 PM Page 9 of 9 Pace Analytical Services, Inc. 9800 Kincey Ave. Suite 100 Huntersville, NC 28078 (704)875-9092 Pace Analytical Services, Inc.

2225 Riverside Dr. Asheville, NC 28804 (828)254-7176 Pace Analytical Services, Inc. 205 East Meadow Road - Suite A

Eden, NC 27288 (336)623-8921

### 3958 6 th

### Ave NW • Seattle, WA 98107 • T: 206-632-6206 • F: 206-632-6017 • www.brooksrand.com • brl@brooksrand.com 

## June 13, 2012
Duke Energy ATTN: Jay Perkins Scientific Support-Laboratory 13339 Hagers Ferry Road Huntersville NC 28078 jcperkins@duke-energy.com labcustomer@duke-energy.com
RE: Project DUK-HV1201 Client Project: J12060052 Dear Mr. Perkins, On June 5, 2012, Brooks Rand Labs (BRL) received two (2) wastewater samples and two (2) corresponding field blanks. Samples were logged-in for total mercury (Hg) analysis according to the chain-of-custody form. All samples were received, prepared, analyzed, and stored according to BRL SOPs and EPA methodology. The results were blank-corrected as described in the calculations section of the applicable SOP(s) and may be evaluated using adjusted reporting limits to account for sample aliquot size. Please refer to the Sample Results page for sample-specific detection limits and other details. The analysis of the third method blank was elevated. The method blank was re-analyzed two more times and the subsequent results were slightly lower with each analysis. Based on the average of the three analyses, the result obtained from the second analysis was reported as – BLK5. None of the method blank results were Grubb’s outliers and the standard deviation exceeded the acceptance limit. This necessitated the elevation of the batch detection limits. The estimated MDL was determined by multiplying the standard deviation by a factor of three and the estimated MRL was calculated as three times the estimated MDL. Aside from concentration qualifiers, all data was reported without additional qualification and all associated quality control sample results met the acceptance criteria. BRL, an accredited laboratory, certifies the reported results of all analyses for which BRL is NELAP accredited meet all NELAP requirements. For more details, see the Report Information page of the report. Please feel free to contact me if you have any questions regarding this report. Sincerely,

Tiffany Stilwater Project Manager tiffany@brooksrand.com

### Project ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



## Definition of Data Qualifiers

(Effective 9/23/09) 

## Laboratory Accreditation BRL is accredited by the National Environmental Laboratory Accreditation Program (NELAP) through the State of Florida Department of Health, Bureau of Laboratories (E87982) and is certified to perform many environmental analyses. BRL is also certified by many other states to perform environmental analyses. For a current list of our accreditations /certifications, please visit our website at <http://www.brooksrand.com/default.asp?contentID=586>. Results reported relate only to the samples listed in the report. 

# Report Information

BLK BRL BS CAL CCV CRM D DUP ICV MSD ND NR PS REC RPD RSD SCV SOP 

### method blank Brooks Rand Labs laboratory fortified blank calibration standard continuing calibration verification certified reference material dissolved fraction duplicate initial calibration verification matrix spike duplicate non-detect non-reportable post preparation spike percent recovery relative percent difference relative standard deviation secondary calibration verification standard operating procedure MDL MRL MS



### method detection limit method reporting limit matrix spike

SRM T COC



### standard reference material total recoverable fraction chain of custody record 

## Common Abbreviations

These qualifiers are based on those previously utilized by Brooks Rand, Ltd., those found in the EPA SOW ILM03.0, Exhibit B, Section III, pg. B-18, and the USEPA Laboratory Data Validation Functional Guidelines for Evaluating Inorganic Analyses; USEPA; July 2002. These supersede all previous qualifiers ever employed by BRL. Detected by the instrument, the result is > the MDL but ≤the MRL. Result is reported and considered an estimate. B E 

### An estimated value due to the presence of interferences. A full explanation is presented in the narrative. H 

### Holding time and/or preservation requirements not met. Result is estimated. J 

### Estimated value. A full explanation is presented in the narrative. J-M 

### Duplicate precision (RPD) for associated QC sample was not within acceptance criteria. Result is estimated. J-N 

### Spike recovery for associated QC sample was not within acceptance criteria. Result is estimated. M 

### Duplicate precision (RPD) was not within acceptance criteria. Result is estimated. N 

### Spike recovery was not within acceptance criteria. Result is estimated. R 

### Rejected, unusable value. A full explanation is presented in the narrative. U 

### Result is ≤the MDL or client requested reporting limit (CRRL). Result reported as the MDL or CRRL. X 

### Result is not BLK-corrected and is within 10x the absolute value of the highest detectable BLK in the batch. Result is estimated. 

## Field Quality Control Samples Please be notified that certain EPA methods require the collection of field quality control samples of an appropriate type and frequency; failure to do so is considered a deviation from some methods and for compliance purposes should only be done with the approval of regulatory authorities. Please see the specific EPA methods for details regarding required field quality control samples.

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

### Project ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Sample Information

Report Matrix Type Received Sampled Sample Lab ID 

### 1223007-01 BioReactor 1 Inf 06/01/2012 06/05/2012 Sample Influent 1223007-02 BioReactor 1 Inf Hg Blk 06/01/2012 06/05/2012 Field Blank DIW 1223007-03 BioReactor 2 Eff 06/01/2012 06/05/2012 Sample Effluent 1223007-04 BioReactor 2 Eff Hg Blk 06/01/2012 06/05/2012 Field Blank DIW 

# Batch Summary

Analyte Prepared Analyzed Sequence Batch Lab Matrix Method 

### B120985 06/06/2012 06/08/2012 1200429 Water EPA 1631 Hg



# Sample Results

Sample Sequence Result MDL MRL Unit Batch

Qualifier Analyte Fraction

Report Matrix 

### BioReactor 1 Inf 

### ng/L 298 T 1200429 B120985 Influent 20.0 6.67 1223007-01 Hg
BioReactor 1 Inf Hg Blk 

### ng/L 0.33 T U 1200429 B120985 DIW 1.00 0.33 1223007-02 Hg

BioReactor 2 Eff 

### ng/L 17.4 T 1200429 B120985 Effluent 4.89 1.63 1223007-03 Hg
BioReactor 2 Eff Hg Blk 

### ng/L 0.33 T U 1200429 B120985 DIW 1.00 0.33 1223007-04 Hg

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

### Project ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Accuracy & Precision Summary

Batch: 

### B120985

Analyte Result Units Native Spike REC & Limits RPD & Limits Method: 

### EPA 1631 Lab Matrix: 

### Water

Sample Certified Reference Material (1221029, NIST 1641d 1000x dilution) B120985-SRM1 

### 15.68 103% ng/L 85-115 Hg 16.07
Matrix Spike (1223006-03) B120985-MS1 

### 69.15 108% ng/L 71-125 Hg 88.09

13.28
Matrix Spike Duplicate (1223006-03) B120985-MSD1 

### 67.24 110% ng/L 71-125 240.8% Hg 87.40

13.28



# Method Blanks & Reporting Limits

Batch: 

### B120985
Method: 

### EPA 1631 Matrix: 

### Water Analyte: 

### Hg Result Units Sample 

### B120985-BLK1 ng/L 0.27 B120985-BLK2 ng/L 0.22 B120985-BLK4 ng/L 0.15 B120985-BLK5 ng/L 0.41 MDL: 

### 0. 33 Average: 

### 0.26 Standard Deviation: 

### 0.11 Limit: 

### 0.10 Limit: 

### 0.50 MRL: 

### 0.99

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

### Project ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Instrument Calibration

Sequence: 

### 1200429 Method: 

### EPA 1631 Instrument: 

### THG-05 Date: 

### 06/08/2012 Total Mercury and Mercury Speciation by CVAFS

Analyte: 

### Hg REC & Limits Result True Value Lab ID Units 

### 1200429-IBL1 7.06 pg of Hg 1200429-IBL2 6.12 pg of Hg 1200429-IBL3 5.43 pg of Hg 1200429-IBL4 5.89 pg of Hg 1200429-CAL1 25.00 24.70 pg of Hg 99% 1200429-CAL2 100.0 98.50 pg of Hg 98% 1200429-CAL3 500.0 495.5 pg of Hg 99% 1200429-CAL4 2500 2546 pg of Hg 102% 1200429-CAL5 10000 10190 pg of Hg 102% 1200429-ICV1 1568 1607 pg of Hg 103% 85-115 1200429-CCV1 500.0 513.7 pg of Hg 103% 77-123 1200429-CCB1 11.8 pg of Hg 1200429-CCV2 500.0 506.3 pg of Hg 101% 77-123 1200429-CCV3 500.0 514.7 pg of Hg 103% 77-123 1200429-CCV4 500.0 535.4 pg of Hg 107% 77-123

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

### Project ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Sample Containers

Lab ID: 

### 1223007-01 Report Matrix: 

### Influent Sample Type: 

### Sample Received: 

### 06/05/2012 Sample: 

### BioReactor 1 Inf Collected: 

### 06/01/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71392670 10 Cooler

none n/a 250 mL Bottle FLPE Hg-T

Lab ID: 

### 1223007-02 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 06/05/2012 Sample: 

### BioReactor 1 Inf Hg Blk Collected: 

### 06/01/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71392670 10 Cooler

none n/a 250 mL Bottle FLPE Hg-T

Lab ID: 

### 1223007-03 Report Matrix: 

### Effluent Sample Type: 

### Sample Received: 

### 06/05/2012 Sample: 

### BioReactor 2 Eff Collected: 

### 06/01/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71392670 10 Cooler

none n/a 250 mL Bottle FLPE Hg-T

Lab ID: 

### 1223007-04 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 06/05/2012 Sample: 

### BioReactor 2 Eff Hg Blk Collected: 

### 06/01/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71392670 10 Cooler

none n/a 250 mL Bottle FLPE Hg-T



# Shipping Containers

Cooler Tracking No: 

### 472679671764 via FedEx Temperature: 

### 0.5 °C Coolant Type: 

### Ice Description: 

### cooler Damaged in transit? 

### No Returned to client? 

### No Custody seals present? 

### No Custody seals intact? 

### No COC present? 

### Yes Received: 

### June 5, 2012 8:45

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

## June 12 , 201 2

Jay Perkins Duke Energy Analytical Laboratory Mail Code MGO3A2 (Building 7405) 13339 Hagers Ferry Rd. Huntersville, NC 28078 (704) 875-5 245 Project: HAPS/MACT Testing Belews Creek (LIMS # J1 2060052)

Dear Mr. Perkins, Attached is the report associated with four (4) aqueous samples submitted for selenium speciation analysis on June 4 , 2012

.The samples were received in a sealed cooler at-0.5 ° C on June 5, 2012

.Selenium speciation analysis was performed via ion chromatography inductively coupled plasma collision reaction cell mass spectrometry (IC-ICP-CRC-MS). Any issues associated with the analysis are addressed in the following report. If you have any questions, please feel free to contact me at your convenience.
Sincerely,

Russell Gerads Vice President Applied Speciation and Consulting, LLC

## Applied Speciation and Consulting, LLC Report prepared for: Jay Perkins Duke Energy Analytical Laboratory Mail Code MGO3A2 (Building 7405) 13339 Hagers Ferry Rd. Huntersville, NC 28078 Project: HAPS/MACT Testing Belews Creek (LIMS # J1 2060052)

June 12 , 2012

1. Sample Reception Four (4) aqueous samples in125mL HDPE bottles (provided by Applied Speciation and Consulting) were submitted for selenium speciation analysi s on June 3 , 2012. The samples were received on June 4 , 201 2 in a sealed container at -0.5 ° C. The samples were received in a laminar flow clean hood,void of trace metals contamination and ultra-violet radiation, and was designated a discrete sample identifier. An aliquot of each sample was filtered (0.45μm) and each filtrate w asstored in a secure, monitored cryofreezer (maintained at a temperature of -80o

## C) until selenium speciation analysis could be performed via ion chromatography inductively coupled plasma collision reaction cell mass spectrometry (IC-ICP- CRC -MS).

2. Sample Preparation All sample preparation is performed in laminar flow clean hoods known to be free from trace metals contamination. All applied water for dilutions and sample preservatives are monitored for contamination to account for any biases associated with the sample results. Selenium Speciation Analysis by IC-ICP-CRC-MS Prior to analysis, an aliquot of each sample was filtered with a syringe filter (0.45μm) and injected directly into a sealed autosampler vial. No further sample preparation was performed as any chemical alteration of a sample June shift the equilibrium of the system, resulting in changes in speciation ratios. 3. Sample Analysis All sample analysis is preceded by a minimum of a five-point calibration curve spanning the entire concentration range of interest. Calibration curves are performed at the beginning of each analytical day. All calibration curves, associated with each species of interest, are

## standardized by linear regression resulting in a response factor. All sample results are

instrument blank correctedto account for any operational biases associated with the analytical platform. Prior to sample analysis, all calibration curves are verified using second source standards which are identified as initial calibration verification standards (ICV). Ongoing instrument performance is identified by the analysis of continuing calibration verification standards (CCV) and continuing calibration blanks (CCB) at a minimal interval of every ten analytical runs. Selenium Speciation Analysis by IC-ICP-CRC-MS Eachsample for selenium speciation analysis w asanalyzed by ion chromatography inductively coupled plasma collision reaction cell mass spectrometry (IC-ICP- CRC -MS) on June 8, 2012. An aliquot of eachsample is injected onto an anion exchange column and mobilized by a basic (pH > 7) gradient. The eluting selenium species are then introduced into a radio frequency (RF) plasma where energy-transfer processes cause desolvation, atomization, and ionization. The ions are extracted from the plasma through a differentially-pumped vacuum interface and travel through a pressurized chamber (CRC) containing a reaction gas which preferentially reacts with interfering ions of the same target mass to charge ratios (m/z). A solid-state detector detects ions transmitted through the mass analyzer and the resulting current is processed by a data handling system. Retention times for each eluting species are compared to known standards for species identification.

4. Analytical Issues The overall analyses went well and no significant analytical issues were encountered. All quality control parameters associated with this sample were within acceptance limits. The estimated method detection limits (eMDLs) for selenite, selenate, and selenocyanate are generated from replicate analyses of the lowest standard in the calibration curve. Not all selenium species are present in preparation blanks; therefore, eMDL calculations based on preparation blanks are artificially biased low. The eMDL for methylseleninic acid and selenomethionine is calculated from the average eMDL of selenite, selenate, and selenocyanate. The calibration does not contain methylseleninic acid or selenomethionine due to impurities in these standards which would bias the results for other selenium species.

## If you have any questions or concerns regarding this report, please feel free to contact me.

Sincerely,

Russell Gerads Vice President Applied Speciation and Consulting, LLC

### Sample Results

Sample ID Se(IV) Se(VI) SeCN MeSe(IV) SeMe Unknown Se Species (n) FGD Purge Eff 87.7 69.8 ND (<2.2) 2.8 ND (<1.7) 0.70 (1) BioReactor 1 Inf 19.7 63.3 ND (<0.54) 2.05 ND (<0.43) 0.69 (2) BioReactor 2 Eff 0.46 ND (<0.35) ND (<0.54) ND (<0.43) ND (<0.43) 0 (0) Metals Trip Blk 0.287 ND (<0.014) ND (<0.022) ND (<0.017) ND (<0.017) 0 (0) All results reflect the applied dilution and are reported in μg/L ND = Not detected at the applied dilution SeCN = Selenocyanate MeSe(IV) = Methylseleninic acid SeMe = Selenomethionine Unknown Se Species = Total concentration of all unknown Se species observed by IC-ICP-MS n = number of unknown Se species observed Report Generated by: Russell Gerads Applied Speciation and Consulting, LLC Selenium Speciation Results for Duke Energy Project Name: HAPS/MACT Testing Belews Creek

Contact: Jay Perkins
Date: June 12, 2012 LIMS #J12060052

## Quality Control Summary - Preparation Blank Summary

Analyte (μg/L) PBW1 PBW2 PBW3 PBW4 Mean StdDev eMDL* eMDL 10x eMDL 250x eMDL 1000x Se(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.002 0.015 0.39 1.5 Se(VI) 0.000 0.000 0.000 0.000 0.000 0.000 0.001 0.014 0.35 1.4 SeCN 0.000 0.000 0.000 0.000 0.000 0.000 0.002 0.022 0.54 2.2 MeSe(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.002 0.017 0.43 1.7 SeMe 0.000 0.000 0.000 0.000 0.000 0.000 0.002 0.017 0.43 1.7 eMDL = Estimated Method Detection Limit *Please see narrative regarding eMDL calculations
Quality Control Summary - Certified Reference Materials Analyte (μg/L) CRM True Value Result Recovery Se(IV) LCS 9.57 9.83 102.7 Se(VI) LCS 9.48 9.24 97.5 SeCN LCS 8.92 8.82 98.9 MeSe(IV) LCS 6.47 5.66 87.4 SeMe LCS 9.32 8.17 87.6 Applied Speciation and Consulting, LLC Selenium Speciation Results for Duke Energy Project Name: HAPS/MACT Testing Belews Creek

Contact: Jay Perkins LIMS #J12060052 Date: June 12, 2012 Report Generated by: Russell Gerads

## Quality Control Summary - Matrix Duplicates

Analyte (μg/L) Sample ID Rep 1 Rep 2 Mean RPD Se(IV) FGD Purge Eff 87.7 89.4 88.5 1.9 Se(VI) FGD Purge Eff 69.8 74.6 72.2 6.7 SeCN FGD Purge Eff ND (<2.2) ND (<2.2) NC NC MeSe(IV) FGD Purge Eff 2.8 2.8 2.8 0.5 SeMe FGD Purge Eff ND (<1.7) ND (<1.7) NC NC ND = Not detected at the applied dilution NC = Value was not calculated due to one or more concentrations below the eMDL

Quality Control Summary - Matrix Spike/ Matrix Spike Duplicate Analyte (μg/L) Sample ID Spike Conc MS Result Recovery Spike Conc MSD Result Recovery RPD Se(IV) FGD Purge Eff 5560 6118 108.4 5560 6109 108.3 0.2 Se(VI) FGD Purge Eff 5045 5068 99.0 5045 5087 99.4 0.4 SeCN FGD Purge Eff 4575 3999 87.4 4575 4021 87.9 0.6 Applied Speciation and Consulting, LLC Selenium Speciation Results for Duke Energy Project Name: HAPS/MACT Testing Belews Creek

Contact: Jay Perkins LIMS #J12060052 Date: June 12, 2012 Report Generated by: Russell Gerads