### Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic

Vascular Plants (Lemna gihha 

### G3)

Data Requirement: EPA DP Barcode

EPA MRID

EPA Guideline

Test material: AE Fl 54851 (Mesosulfuron)*

Common name: Mesosulfuron

Chemical name: IUPAC: Not reported 422360 and 422509

49447201

850.4400

Purity: 96.1 % MRID 49447201

CAS name: 2-[3-(4,6-Dimethoxypyrimidin-2-yl)ureidosulfonyl]-4-methanesulfonamidomethylbenzoic acid

CAS No.: Not reported

Synonyms: AE Fl5485, mesosulfuron

* AE F 154851 is a degradate of mesosulfuron-methyl

Primary Reviewer: Kindra Bozicevich

Environmental Scientist, COM Smith

Secondary Reviewer: Teri S. Myers

Senior Scientist, COM Smith

Primary Reviewer: Michael Lowit, Ph.D.

Ecologist, EPA/OCSPP / OPP/EFED/ERB 1

EPA PC Code: 122009

Date Evaluation Completed: 4/10 / 2015 Signature:

## ~ "f>'t:fVicL

Date: 10/21/14

Signature:

## ·5**~** Date: 01/13 / 15

# Signature:**,$~ :LI/~**  Date: 411 

## orfo1 

# "f 1

CITATION: Dorgerloh, M

. 2005. Lemna gibba G3- Growth Inhibition Test with AE F 154851 under Static Conditions

(code: AE Fl54851001896 0001). Unpublished study performed by BayerCropScienceAG, Monheim, Germany.

Report ID EBMMX090. Laboratory Project IDE 412 2878-2. Study sponsored by Bayer CropScience AG. Study

initiated 0 l February 2005 and completed 28 July 2005.

DISCLAIMER: This document provides guidance for EPA and PMRA reviewers on how to complete a data

evaluation record after reviewing a scientific study concerning the acute toxicity of a pesticide to aquatic vascular

plants. It is not intended to prescribe conditions to any external party for conducting this study nor to establish

absolute criteria regarding the assessment of whether the study is S\,:ientifically sound and whether the study satisfies

any applicable data requirements

. Reviewers are expected to review and to determine for each study, on a case-bycase basis, whether it is scientifically sound and provides sufficient information to satisfy applicable data

requirements. Studies that fail to meet any of the conditions may be accepted, if appropriate; similarly, studies that

meet all of the conditions may be rejected, if appropriate. In sum, the reviewer is to take into account the totality of

factors related to the test methodology and results in determining the acceptability of the study

.

### Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic

Vascular Plants ( Lemna gibba G3)

MRID 49447201

EXECUTIVE SUMMARY

In a 7

- day toxicity study, aquatic vascular plants ( duc kweed, Lemna gibba G3 ) were exposed to m esosulfuron (AE

F154851) , a degradate of mesosulfuron

- methyl

, at nomina l concentrations of 0 (negative control ) , 0.003, 0.01, 0.03, 0.10,

0.31, 0.98, 3.13, and 10.0 m g /L under static conditions. Mean m e asured concen trations were <0.001349 ( negative

control ) 0.003, 0.01, 0 .03, 0.10, 0.33, 1.03, 3.13, and 6.12 mg ai/L

. NOAEC values based on all endpoints ( frond

number y ield , frond number growth rate, frond area yield , and fond area growth rate) were 0.03 mg ai /L, in t erms of mean

measured concentrations

. IC 50 values were 0.0506 mg ai/L for frond number yield, 0.10 9 mg ai/L for frond number

growth rate, 0.046 9 mg ai/L for frond area yield, and 0.084 2 mg ai/L for frond area growth rate, in terms of mean

measured concent rations. The % growth inhibition of frond number in the treated culture as compared to the negative

con trol ranged from

- 7.8 to 90.4 % after 7 days

.

Fronds that grew together were observed in the 0.10, 0.31, 0.98, and 3.13 mg/L treatment groups. S lightly chlorotic

fronds were observed in the 3.13 and 10 mg/L treatment group s. The pH increased from 7

- 7.5 (day 0) to 8.4

- 8.9

(day 7) during the study

.

This study is scientifically sound and is classified as Acceptable

.

Results Synopsis

Frond number yield

IC 05 : 0.002691 mg ai/L 95% C.I.: N/A to 0.01311 mg ai/L

IC 50 : 0.05061 m g ai/L 95% C.I.: 0.0272 to 0.09415 mg ai/L

NOAEC: 0.03 m g ai/L

Probit Slope: N/A

Frond number growth rate

IC 05 : 0.00144 mg ai/L 95% C.I.: N/A to 0.008465 mg ai/L

IC 50 : 0.1089 m g ai/L 95% C.I.: 0.06598 to 0.1797 mg ai/L

NOAEC: 0.03 m g ai/L

Probit Slope: N/A

Frond area yield

IC 05 : 0.002842 mg ai/L 95% C.I.: N/A to 0.01232 mg ai/L

IC 50 : 0.04688 m g ai/L 95% C.I.: 0.02631 to 0.08352 mg ai/L

NOAEC: 0.03 m g ai/L

Probit Slope: N/A

Frond area growth rate

IC 05 : 0.001394 mg ai/L 95% C.I.: N/A to 0.00685 mg ai/L

IC 50 : 0.08417 m g ai/L 95% C.I.: 0.05415 to 0.1308 mg ai/L

NOAEC: 0.03 m g ai/L

Probit Slope: N/A

Endpoint s affected: f rond number yield, frond number growth rate, f rond are a yield, frond area growth rate

Page 2 of 12

### Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic

Vascular Plants ( Lemna gibba G3)

MRID 49447201

I. MATERIALS AND METHODS

GUIDELINE FOLLOWED: The study was designed to comply with the procedures of the OECD

Guideline 221 (2004 )

.

The following deviations from the U.S. EPA OCSPP 850.4400 (2012) guideline w ere noted :

1. The health of the inoculum culture was not reported. The inoculum should be from a logarithmically

growing stock culture.

2. The pH, total organic carbon (TOC), particulate matter, metals, pesticides, and chlorine concentrations

of the dilution w ater were not reported.

3. The number of plants/replicate and the number of fronds/plant were not reported; a total of 12 fronds

per vessel were used.

4. Measured light intensity was 7360

- 10,000 lux; recommend light intensity is 4200

- 6700 lux.

5. Only 3 replicates were used per test level. A minimum of 4 replicates per level is recommended.

These deviations do not affect the validity of the study.

COMPLIANCE: S igned and dated GLP, Quality Assurance

, and No Data Confidenti ality

statements were provided. The s tudy was performed in accordance with

OECD Principles of GLP, the Principles of GLP according to Annex 1 of

the German chemical law (ChemG) , USEPA 40 CFR Part 160, and JMAFF

11 Nousan No. 6283.

A. MATERIALS

1. Test material Mesosulfuron ( AE F154851 )

Description White powder

Lot No./Batch No. LOR 21029

Purity 96.1 %

Stability of compound

under test conditions Stable. Day 7 measur e d concentrations ranged from 97 to 10 0 % of their

initial measured counterparts.

Storage conditions o f

test chemicals Stored at

- 20 ± 5 ° C

.

Table 1. Physicochemical properties of Mesosulfuron

Parameter Values Comments

Water solubility at 20 ° C Not reported

Vapor pressure Not reported

UV absorption Not reported

Page 3 of 12

### Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic

Vascular Plants ( Lemna gibba G3)

MRID 49447201

Parameter Values Comments

pKa Not reported

Kow Not reported

2. Test organism

Name: Duckweed, Lemna gibba

Strain: G3

Source: In

- house cultures originally obtained from Dr. Janet Slovin, Horticulture

Crops Quality Laboratory, U.S. Department of Agriculture, Be ltsville, MD

20705 U.S.A.

Age of inoculum : 7

- 10 days

Method of cultivation: Cultures were maintained in glass dishes filled with 20X AAP medium

under illumination of 7360

- 10 ,000 lux and temperature of 24 ± 2 ° C for a

minimum of three weeks. Transfers were made regularly into fresh medium

to provide 7

- 10 days old colonies as test inoculum.

B. STUDY DESIGN

1. Experimental Conditions

a. Range

- find ing study

The range of test concentrations w as selected based on pre experiments (data not reported)

.

b. Defi nitive s tudy

Table 2. Experimental Parameters

Parameter Details Remarks

Criteria

Acclimation period

C ulturing media and conditions

Health Continuously cultured in

- house

(maintained under test conditions at

least three weeks)

Same as test

Not re ported

Test system

Static/static renewal Static Test concentrations were stable over

the exposure period.

Static renewal intervals should be

sufficient to maintain test

concentrations.

Page 4 of 12

### Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic

Vascular Plants ( Lemna gibba G3)

MRID 49447201

Parameter Details Remarks

Criteria

Incubation facility Growth incubator

Duration of the test 7 days

7 days

Test vessel

Material (glass/stainless steel)

Size

Fill volume Glass

4 7 0 mL

20 0 mL Glass dishes (10 cm diameter , 6 cm

in height) covered with glass li ds.

Details of 20X AAP medium

pH in new solu tions

pH in old solutions

Chelator used

Carbon source 7.0 to 7.5

8.4 to 8.9

Na 2 EDTA

· 2H 2 O

NaHCO 3 EPA recommends the following

culture media: 20X

- AAP for Lemna

gibba. Chelating agents (e.g. EDTA)

are recomm ended in the nutrient

medium for optimum cell growth.

Lower concentrations of chelating

agents (down to one

- third of the

normal concentration recommended

for AAP medium) may be used in the

nutrient medium used for test

solution preparation if it is

suspect ed that the chelator will

interact with the test material.

ASTM reference, E1415

- 91and D

3978

- 80 (reapproved 1987).

If non

- standard nutrient medium

was used, detailed composition

provided

. A standar d medium was used. A

detailed comp osition was provided

.

Dilution water

Source/type

pH

Water pretreatment

Total organic carbon

P articulate matter

Metals

Pesticides

Chlorine Ultra

- pure water

Not reported

Purified (Milli

- Q

- water)

Not reported

Not reported

Not reporte d

Not reported

Not reported pH of 7.5 ± 0.1 is acceptable if 20X

AAP nutrient media is used.

Page 5 of 12

### Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic

Vascular Plants ( Lemna gibba G3)

MRID 49447201

Parameter Details Remarks

Criteria

Indicate how the test material is

added to the medium (added

directly or used stock solution) A stock solution was prepared by

dissolvin g 104.5 mg of the test

substance in 1000 mL of test

medium. The resulting solution was

stirred and sonicated, then

transferred to a dilution series to

obtain the concentration levels used

in the study.

Aeration or agitation None

Sediment used ( for rooted aquatic

vascular plants)

Origin

Textural classif ication (%sand, silt,

and clay)

Organic carbon (%)

Geographic location N/A

Number of replicates

Nega tive control

Solvent control

Treatments 3

N/A

3 4 minimum

Number of plants/replicate Not reported

3

- 5 plants

Number of fronds/plant Not reported (total of 12 fronds per

vessel )

3

- 4 fronds per plant

Page 6 of 12

### Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic

Vascular Plants ( Lemna gibba G3)

MRID 49447201

Parameter Details Remarks

Criteria

Test concentrations

Nominal

Mean measured * 0 (negative control ) 0.003, 0 .01,

0.03, 0.10, 0.31, 0.98, 3.13, and 10.0

mg /L

<0.001349 ( negative control ) 0.003,

0.01

, 0.03 , 0. 10 , 0.3 3 , 1.03, 3.13,

and 6.12 mg ai /L *Mean measured concentrations

were determined by the reviewer as

the mean of Day 0 and Day 7

measured concentrations.

Con centrations were not

analytically

- determined for the

lowest two test levels ( nominal

0.003 and 0.01 mg/L ). The reviewer

estimated concentrations for the

lowest two test levels based on the

average mean re covery for the other

test levels (96.2% of nomi nal )

.

EPA requires at least 5 test

concentrations except for limit tests.

Solvent Not used

Method and interval of analytical

verification Test concentrations were measured

in newly prepared solutions on Day

0 and from expired solutions on Day

7 using HPLC

- MS/MS

.

Test conditions

Temperature

Photoperiod

Light intensity and quality 2 3.7 to 24.0 o C

Continuous

7630 to 10

, 000 lux (quality not

reported)

Reference chemical Not used

Other parameters None

2. Observations

Table 3. Observation parameters

Parameters Details Remarks/Criteria

Parameters measured (e.g., number

of fronds, plant dry weight or other

toxicity symptoms) Frond number

Frond area

Growth rate

Page 7 of 12

### Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic

Vascular Plants ( Lemna gibba G3)

MRID 49447201

Parameters Details Remarks/Criteria

Measurement technique for frond

number and other end points Counting of fronds and

determination of total frond area

was carried out using a

Lemna Tec Scanalyzer machine.

Observation intervals Fronds were counted on days 0,

2, 5, and 7

.

Other observations, if any Visual observati ons were made

on study days 2 , 5 , and 7.

Indicate whether there was

exponential growth in the control Yes, the doubling time in the

negative control was less than 2

days.

Were raw data included? Yes

II. RESULTS and DISCUSSION

A. INHIBITORY EFFECTS

After 7 days, the mean frond number of the ne gative control was 167 fronds , yielding inhibitions relative to the

negative control of 0.0, 5.0,

- 7.8, 76.0, 87.0, 88.2, 89.4, and 90.4 % for the mean measu red concentration s of

0.003, 0.01, 0.03, 0.10, 0.33, 1.03, 3.13, and 6.12 m g ai /L , respectively

. The study author did not assess frond

number data.

The mean 0

- 7 day frond number growth rate o f the negative control was 0.376 /day, yielding inhibitions rela tive

to the negative control of

- 0.1, 2.0,

- 2.9, 54.3, 77.7, 81.3, 85.7, and 89.2 % for the mean measured concentration s

of 0.003, 0.01, 0.03, 0.10, 0.33, 1.03, 3.13, and 6.12 m g ai /L , respectively

. The NOAEC and I C 50 values

reported by the study author ba sed on fro nd number growth rate were 0.03 and 0.12 m g/ L, respectively, in terms

of nominal concentrations.

The mean 0

- 7 day frond number yield of the negative control was 155 f ronds, yielding inhibitions relative to the

negative control of 0.0, 5.4,

- 8.4, 81.9, 93.8, 95.1, 96.3, and 97.4 % f or the mean measured concentration s of

0.003, 0.01, 0.03, 0.10, 0.33, 1.03, 3.13, and 6.12 m g ai /L , respectively

. The study author did not assess frond

number yield data.

After 7 days, the mean frond area of the negati ve control w as 1377 mm 2 , yielding inhibitions relative to the

negative control of

- 1.2, 8.2,

- 4.8, 77.7, 86.2, 87.3, 89.4, and 90.7 % for the mean measured concentration s of

0.003, 0.01, 0.03, 0.10, 0.33, 1.03, 3.13, and 6.12 m g ai /L , respectively

. The stu dy author did not assess frond

area data.

The mean 0

- 7 day frond area growth rate of the negative control was 0.367 /day, yielding inhibitions relative to

the negative control of 0.9, 3.1, 1.9, 60.9, 79.4, 85.5, 89.3, and 91.8 % for mean measured concentrat ion s of

0.003, 0.01, 0.03, 0.10, 0.33, 1.03, 3.13, and 6.12 m g ai/L , respectivel y

. The NOAEC and IC 50 values reported

by the study author based on frond area growth rate were 0.03 and 0.11 m g/L, respectively, in terms of nominal

concentrations.

Page 8 of 12

### Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic

Vascular Plants ( Lemna gibba G3)

MRID 49447201

The mean 0

- 7 day frond area yield of the negative control was 1271 mm 2 , yielding inhibitions relative to the

negative control of

- 1.0, 8.8,

- 4.4, 84.7, 93.9, 95.8, 97.2, and 98.1 % for the mean measured concentration s of

0.003, 0.01, 0.03, 0.10, 0.33, 1.03, 3.13, an d 6.12 m g ai/L

, respectively . The study author did not calculate or

assess frond area yield data.

Fronds that grew together were observed in the 0.10, 0.31, 0.98, and 3.13 mg/L treatment groups. Slightly

chlorotic fronds were observed in the 3.13 and 10 mg/L treatment groups. The pH increased from 7

- 7.5 (day 0)

to 8.4

- 8.9 (day 7) during the study

.

Table 4. Effect of m esosulfuron on frond number of duckweed, Lemna gibba

Treatment

Mean measured

and (n ominal )

concentrations

m g ai /L Initial frond

number /test

solution frond number at

2 days 5 days 7 days

frond number % inhibition a

Negative control 12 28 83 167 N/A

0.003(0.003) 12 27 87 167 0.0

0.01(0.01) 12 24 81 159 5.0

0.03(0.03) 12 27 92 180

- 7.8

0.10(0.10) 12 21 27 40 76.0

0.33(0. 31) 12 18 21 22 87.0

1.03(0.98) 12 19 20 20 88.2

3.13(3.13) 12 15 18 18 89.4

6.12(10) 12 13 15 16 90.4

a Calculated by the reviewer relative to the negative control.

Table 5. Effect of m esosulfuron on frond number of duckweed, Lemna gibba

Treatment

M ean measured

and (nominal)

concentrations

m g ai /L Initial frond

number/test

solution Frond number growth rate

(day

- 1 ) Frond number yield a

0

- 7 days % inhibition 0

- 7 days % inhibition b

Negative control 12 0.376 N/A 155 N/A

0.003(0.003) 12 0.376

- 0.1 155 0.0

0.01(0.01) 12 0.368 2.0 147 5.4

0.03(0.03) 12 0.387

- 2.9 168

- 8.4

0.10(0.10) 12 0.172 54.3 28 81.9

0.33(0.31) 12 0.084 77.7 10 93.8

1.03(0.98) 12 0.070 81.3 8 95.1

3.13(3.13) 12 0.054 85.7 6 96.3

6.12(10) 12 0.041 89.2 4 97.4

a Calculated b y the reviewer as final minus initial frond number

b Calculated by the reviewer relative to the negative control.

Page 9 of 12

### Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic

Vascular Plants ( Lemna gibba G3)

MRID 49447201

Table 6. E ffect of m esosulfuron on biomass of duckweed, Lemna gibba

Treatment

Mean measured

and (nominal)

concentratio ns

m g ai/L Frond area (mm 2 ) Frond area growth rate

(day

- 1 ) Frond area yield a

Day 7 % inhibition b 0

- 7 days % inhibition 0

- 7 days % inhibition b

Negative control 1377 N/A 0.367 N/A 1271 N/A

0.003(0.003) 1393

- 1.2 0.363 0.9 1284

- 1.0

0.01(0.01) 1265 8. 2 0.355 3.1 1160 8.8

0.03(0.03) 1443

- 4.8 0.360 1.9 1327

- 4.4

0.10(0.10) 307 77.7 0.143 60.9 194 84.7

0.33(0.31) 190 86.2 0.075 79.4 78 93.9

1.03(0.98) 175 87.3 0.053 85.5 54 95.8

3.13(3.13) 146 89.4 0.039 89.3 35 97.2

6.12(10) 128 90.7 0.030 91.8 25 98.1

a Calculated by the reviewer as the final minus initial frond area.

b Calculated by the reviewer relative to the negative control.

Table 7. Statistical endpoint values (calculated by the study author based on nominal concentr ations)

Statistical

Endpoint Frond number Frond number

growth rate Frond area Frond area

growth rate

NOAEC ( mg /L) Not calculated 0.03 Not calculated 0.03

LOAEC ( mg /L) Not calculated 0.10 Not calculated 0.10

IC 50 or EC 50 ( mg /L) (95% C.I.) Not calculate d 0.12 (0.04

- 0.34) Not calculated 0.11 (0.006

- 0.23)

Reference chemical

NOAEC

IC 50 /EC 50 N/A

N/A. Not applicable.

B. REPORTED STATISTICS

The study author statistically analyzed the endpoints for frond number growth rate and frond area growth rate using

T oxRat Professional statistical software. The data were assessed for normality using the r ange

- to

- standard

deviation

- ratio test and for homogeneity of variance u sing Cochran’s test or Welch t

- test , respectively. The

treatments were compared to the control using Dunnett’s Multiple t

- test. The ECx values were determined using

Probit analysis

. All analyses were based on nominal concentrations.

Page 10 of 12

### Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic

Vascular Plants ( Lemna gibba G3)

MRID 49447201

C. VERI FICATION OF STATISTICAL RESULTS

The reviewer assessed the endpoints fo r frond number yield , frond num ber growth rate, frond area yield

, and

frond area growth rate using CETIS version 1.8.7.12 statistical software using backend database settings

implemented by EFED on 25 March 2014

. Frond area growth rate and frond number growth rate data were

confirmed to be normally distributed and have homogeneous variances using Shapiro

- Wilk’s and Bartlett’s tests,

respectively, and the data were therefore an alyzed using Dunnett ’s tests. Frond area yield data had non

- normal

distribution and unequal variances, and the data were therefore analyzed using Mann

- Whitney tests. Frond

number yield data were normally distributed, but had unequal variances and the data were therefore analyzed

using Mann

- Whitney tests. The ICx values were calculated using Bruce

- Versteeg regression. All toxicity

values are reported in terms of mean measured exposure concentrations.

Frond number yield

IC 05 : 0.002691 mg ai/L 95% C.I.: N/A to 0.01311 mg ai/L

IC 50 : 0.05061 m g ai/L 95% C.I.: 0.0272 to 0.09415 mg ai/L

NOAEC: 0.03 m g ai/L

Probit Slop e: N/A

Frond number growth rate

IC 05 : 0.00144 mg ai/L 95% C.I.: N/A to 0.008465 mg ai/L

IC 50 : 0.1089 m g ai/L 95% C.I.: 0.06598 to 0.1797 mg ai/L

NOAEC: 0.03 m g ai/L

Probit Slope: N/A

Frond area yield

IC 05 : 0.002842 mg ai/L 95% C.I.: N/A to 0.012 32 mg ai/L

IC 50 : 0.04688 m g ai/L 95% C.I.: 0.02631 to 0.08352 mg ai/L

NOAEC: 0.03 m g ai/L

Probit Slope: N/A

Frond area growth rate

IC 05 : 0.001394 mg ai/L 95% C.I.: N/A to 0.00685 mg ai/L

IC 50 : 0.08417 m g ai/L 95% C.I.: 0.05415 to 0.1308 mg ai/L

N OAEC: 0.03 m g ai/L

Probit Slope: N/A

D. STUDY DEFI CIENCIES

See guideline deviations.

E. REVIEWER ’ S COMMENTS

The reviewer’s results were in agreement with those of the study author. The study author’s toxicity values were

based on nominal concentr ations whereas the reviewer’s values were based on mean measured concentrations. The

study author did not analyze f r ond number yield or frond area yield data. The reviewer’s results are presented in the

Executive Summary and Conclusions sections of this report.

The laboratory portion of the defin itive test was conducted from 23 February to 02 March 20 05

.

T he reviewer was unable to calculate time

- weighted average (TWA) concentrations. Test concentrations were only

Page 11 of 12

### Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic

Vascular Plants ( Lemna gibba G3)

MRID 49447201

measured in newly prepared solutions on Day 0 and from expired solutions on Day 7.

The coefficient of variation (CV) of the negative control based on yield was 9.03

- 11.03% and based on growth rate

was 3.16

- 3.18%. The guideline requirement for both yield and growth rate is < 20%.

F. CONCLUSI ONS

This study is scientifically sound and is classified as Acceptable

.

Frond number yield

IC 05 : 0.002691 mg ai/L 95% C.I.: N/A to 0.01311 mg ai/L

IC 50 : 0.05061 m g ai/L 95% C.I.: 0.0272 to 0.09415 mg ai/L

NOAEC: 0.03 m g ai/L

Probit Slope: N/A

F rond number growth rate

IC 05 : 0.00144 mg ai/L 95% C.I.: N/A to 0.008465 mg ai/L

IC 50 : 0.1089 m g ai/L 95% C.I.: 0.06598 to 0.1797 mg ai/L

NOAEC: 0.03 m g ai/L

Probit Slope: N/A

Frond area yield

IC 05 : 0.002842 mg ai/L 95% C.I.: N/A to 0.01232 mg ai/L

IC 50 : 0.04688 m g ai/L 95% C.I.: 0.02631 to 0.08352 mg ai/L

NOAEC: 0.03 m g ai/L

Probit Slope: N/A

Frond area growth rate

IC 05 : 0.001394 mg ai/L 95% C.I.: N/A to 0.00685 mg ai/L

IC 50 : 0.08417 m g ai/L 95% C.I.: 0.05415 to 0.1308 mg ai/L

NOAEC: 0.03 m g ai/L

Probit Slope: N/A

Endpo ints a ffected: f rond number yield, frond number growth rate, frond area yield, frond area growth rate

.

III. REFERENCES

Revised Proposal for a New Guideline OECD 221 “ Lemna sp. Growth Inhibition Test” ( April 2004 ).

Stat istical Software “To xRa t Professional”, version 2.09 , produced by ToxRat  Solutions GmbH, 52477 Al sdorf,

Germany ( January 27, 2005 ).

ToxRat  Validation Document from ToxRat  Solutions GmbH, valid for ToxRat  Version 2 .09 (released January 25,

2004)

.

M. D orgerloh, “LemnaTec Scanalyzer Validation Program”, Bayer CropScience AG, unpublished report DOM 23038 of

April 7, 2004.

Page 12 of 12

### CETIS Summary Report Report Date: 27 Oct-14 19:19 (p 1 of 2)

Test Code: 122009 49447201 | 13-6193-9276

OCSPP 850.4400 Aquatic Vascular Plant Bayer CropScience AG

Batch ID: 04-2560-9572

Start Date: 23 Feb-05

Ending Date: 02 Mar-05 Test Type: Lemna Growth (7-d)

Duration: 7d 0h Protocol: OCSPP 850.4400 Aquatic Plant (Lemna) Diluent: Lemna Medium, 20X-AAP

Brine: Species: Lemna Gibba

Source: Lab In-House Culture Analyst:

Age: 7-10

Sample ID: 05-6897-8840

Sample Date: 23 Feb-05

Receive Date: Code: 49447201

Sample Age: NA Source: Bayer CropScience AG

Station: Client: CDM Smith

Project: Unknown Material: Mesosulfuron-methyl

122009 49447201 static

122009 49447201 using mesosulfuron Batch Note:

Sample Note:

Comparison Summary

NOEL LOEL TOEL

Endpoint Analysis ID Method PMSD TU

0.03 0.1 0.05477 11-9691-0524 Dunnett Multiple Comparison Test 4.68% Area Growth Rate

0.03 0.1 0.05477 16-1377-2374 Williams Multiple Comparison Test 3.41% Area Growth Rate

0.1 0.33 0.1817 13-0619-1884 Jonckheere-Terpstra Step-Down Test NA Frond Area

0.03 0.1 0.05477 03-4998-1283 Mann-Whitney U Two-Sample Test 11.6% Frond Area

0.1 0.33 0.1817 16-6090-3627 Jonckheere-Terpstra Step-Down Test NA Frond Number

0.03 0.1 0.05477 04-2583-3152 Mann-Whitney U Two-Sample Test 9.81% Frond Number

0.03 0.1 0.05477 20-6095-1587 Dunnett Multiple Comparison Test 7.72% Frond Number Growth Rate

0.03 0.1 0.05477 18-7448-1143 Williams Multiple Comparison Test 5.62% Frond Number Growth Rate

95% LCL 95% UCL Point Estimate Summary

Endpoint Analysis ID Method mg ai/L TU Level

IC5 0.001394 N/A 0.00685 Area Growth Rate 03-6489-1028 Nonlinear Regression

IC10 0.003448 0.000266 0.009393

IC25 0.01566 0.007658 0.02847

IC50 0.08417 0.05415 0.1308

IC5 0.002842 N/A 0.01232 Frond Area 05-8526-2936 Nonlinear Regression

IC10 0.005278 N/A 0.01459

IC25 0.01485 0.005427 0.03006

IC50 0.04688 0.02631 0.08352

IC5 0.002691 N/A 0.01311 Frond Number 07-6323-2088 Nonlinear Regression

IC10 0.005145 N/A 0.01541

IC25 0.01519 0.005086 0.03238

IC50 0.05061 0.0272 0.09415

IC5 0.00144 N/A 0.008465 Frond Number Growth Rat 06-6140-0687 Nonlinear Regression

IC10 0.003744 9.09E-05 0.01152

IC25 0.01848 0.008031 0.0366

IC50 0.1089 0.06598 0.1797

CETISTM v1.8.7.12 000-516-187-1 QA:________ Analyst:________

### CETIS Summary Report Report Date: 27 Oct-14 19:19 (p 2 of 2)

Test Code: 122009 49447201 | 13-6193-9276

OCSPP 850.4400 Aquatic Vascular Plant Bayer CropScience AG

Control Type Mean Min Max Std Dev

Count CV% Std Err C-mg ai/L Area Growth Rate Summary

95% LCL 95% UCL %Effect

0 Negative Control 0.3663 0.354 0.377 0.01159 3 3.16% 0.006692 0.3375 0.3951 0.0%

0.003 0.3637 0.36 0.366 0.003215 3 0.88% 0.001856 0.3557 0.3717 0.73%

0.01 0.355 0.338 0.374 0.01808 3 5.09% 0.01044 0.3101 0.3999 3.09%

0.03 0.36 0.355 0.367 0.006245 3 1.74% 0.003606 0.3445 0.3755 1.73%

0.1 0.1433 0.136 0.149 0.006658 3 4.65% 0.003844 0.1268 0.1599 60.87%

0.33 0.07533 0.073 0.078 0.002517 3 3.34% 0.001453 0.06908 0.08158 79.44%

1.03 0.05267 0.05 0.057 0.003786 3 7.19% 0.002186 0.04326 0.06207 85.62%

3.13 0.03933 0.038 0.041 0.001528 3 3.88% 0.000882 0.03554 0.04313 89.26%

6.12 0.031 0.026 0.034 0.004359 3 14.06% 0.002517 0.02017 0.04183 91.54%

Control Type Mean Min Max Std Dev

Count CV% Std Err C-mg ai/L Frond Area Summary

95% LCL 95% UCL %Effect

0 Negative Control 1271 1118 1393 140.2 3 11.03% 80.95 923 1620 0.0%

0.003 1284 1248 1304 31.24 3 2.43% 18.04 1206 1362 -1.0%

0.01 1160 977 1362 193.2 3 16.66% 111.5 680.1 1640 8.76%

0.03 1327 1236 1381 79.27 3 5.97% 45.76 1130 1524 -4.38%

0.1 194.3 176 206 16.07 3 8.27% 9.28 154.4 234.3 84.71%

0.33 78 75 81 3 3 3.85% 1.732 70.55 85.45 93.86%

1.03 54 48 60 6 3 11.11% 3.464 39.1 68.9 95.75%

3.13 35.33 32 37 2.887 3 8.17% 1.667 28.16 42.5 97.22%

6.12 24.67 22 26 2.309 3 9.36% 1.333 18.93 30.4 98.06%

Control Type Mean Min Max Std Dev

Count CV% Std Err C-mg ai/L Frond Number Summary

95% LCL 95% UCL %Effect

0 Negative Control 155 139 165 14 3 9.03% 8.083 120.2 189.8 0.0%

0.003 155 149 160 5.568 3 3.59% 3.215 141.2 168.8 0.0%

0.01 146.7 130 164 17.01 3 11.6% 9.821 104.4 188.9 5.38%

0.03 168 161 182 12.12 3 7.22% 7 137.9 198.1 -8.39%

0.1 28 26 30 2 3 7.14% 1.155 23.03 32.97 81.94%

0.33 9.667 8 12 2.082 3 21.53% 1.202 4.496 14.84 93.76%

1.03 7.667 6 9 1.528 3 19.92% 0.8819 3.872 11.46 95.05%

3.13 5.667 3 9 3.055 3 53.91% 1.764 -1.922 13.26 96.34%

6.12 4 2 5 1.732 3 43.3% 1 -0.3027 8.303 97.42%

Control Type Mean Min Max Std Dev

Count CV% Std Err C-mg ai/L Frond Number Growth Rate Summary

95% LCL 95% UCL %Effect

0 Negative Control 0.3757 0.362 0.384 0.01193 3 3.18% 0.006888 0.346 0.4053 0.0%

0.003 0.376 0.371 0.38 0.004583 3 1.22% 0.002646 0.3646 0.3874 -0.09%

0.01 0.3683 0.353 0.384 0.0155 3 4.21% 0.00895 0.3298 0.4068 1.95%

0.03 0.3867 0.381 0.398 0.009815 3 2.54% 0.005667 0.3623 0.411 -2.93%

0.1 0.172 0.165 0.179 0.007 3 4.07% 0.004041 0.1546 0.1894 54.21%

0.33 0.084 0.073 0.099 0.01345 3 16.02% 0.007767 0.05058 0.1174 77.64%

1.03 0.07033 0.058 0.08 0.01124 3 15.98% 0.006489 0.04241 0.09825 81.28%

3.13 0.054 0.032 0.08 0.02425 3 44.91% 0.014 -0.00624 0.1142 85.63%

6.12 0.04067 0.022 0.05 0.01617 3 39.75% 0.009333 0.000509 0.08082 89.17%

CETISTM v1.8.7.12 000-516-187-1 QA:________ Analyst:________