Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic 

Vascular Plants (Lemna gihha G3) 

Data Requirement: EPA DP Barcode 

EPA MRID 

EPA Guideline 

Test material: AE Fl 54851 (Mesosulfuron)* 

Common name: Mesosulfuron 

Chemical name: IUPAC: Not reported 

422360 and 422509 

49447201 

850.4400 

Purity: 96.1 % 

MRID 49447201 

CAS name: 2-[3-(4,6-Dimethoxypyrimidin-2-yl)ureidosulfonyl]-4-methanesulfonamidomethylbenzoic acid 

CAS No.: Not reported 

Synonyms: AE Fl5485, mesosulfuron 

* AE F 154851 is a degradate of mesosulfuron-methyl 

Primary Reviewer: Kindra Bozicevich 

Environmental Scientist, COM Smith 

Secondary Reviewer: Teri S. Myers 

Senior Scientist, COM Smith 

Primary Reviewer: Michael Lowit, Ph.D. 

Ecologist, EPA/OCSPP/OPP/EFED/ERB 1 

EPA PC Code: 122009 

Date Evaluation Completed: 4/10/2015 

Signature: ~ "f>'t:fVicL 

Date: 10/21/14 

Signature: 

Date: 01/13/15 

·5 ~ 

Signature: ,$~ :LI/~ Date: 411 orfo1 "f 1 

CITATION: Dorgerloh, M. 2005. Lemna gibba G3- Growth Inhibition Test with AE F 154851 under Static Conditions 

(code: AE Fl54851001896 0001). Unpublished study performed by BayerCropScienceAG, Monheim, Germany. 

Report ID EBMMX090. Laboratory Project IDE 412 2878-2. Study sponsored by Bayer CropScience AG. Study 

initiated 0 l February 2005 and completed 28 July 2005. 

DISCLAIMER: This document provides guidance for EPA and PMRA reviewers on how to complete a data 

evaluation record after reviewing a scientific study concerning the acute toxicity of a pesticide to aquatic vascular 

plants. It is not intended to prescribe conditions to any external party for conducting this study nor to establish 

absolute criteria regarding the assessment of whether the study is S\,:ientifically sound and whether the study satisfies 

any applicable data requirements. Reviewers are expected to review and to determine for each study, on a case-bycase basis, whether it is scientifically sound and provides sufficient information to satisfy applicable data 

requirements. Studies that fail to meet any of the conditions may be accepted, if appropriate; similarly, studies that 

meet all of the conditions may be rejected, if appropriate. In sum, the reviewer is to take into account the totality of 

factors related to the test methodology and results in determining the acceptability of the study. 
Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic 

Vascular Plants (Lemna gibba G3)

MRID 49447201

EXECUTIVE SUMMARY

In a 7-day toxicity study, aquatic vascular plants (duckweed, Lemna gibba G3) were exposed to mesosulfuron (AE 

F154851), a degradate of mesosulfuron-methyl, at nominal concentrations of 0 (negative control), 0.003, 0.01, 0.03, 0.10, 

0.31, 0.98, 3.13, and 10.0 mg/L under static conditions. Mean measured concentrations were <0.001349 (negative 

control) 0.003, 0.01, 0.03, 0.10, 0.33, 1.03, 3.13, and 6.12 mg ai/L. NOAEC values based on all endpoints (frond 

number yield, frond number growth rate, frond area yield, and fond area growth rate) were 0.03 mg ai/L, in terms of mean 

measured concentrations. IC50 values were 0.0506 mg ai/L for frond number yield, 0.109 mg ai/L for frond number 

growth rate, 0.0469 mg ai/L for frond area yield, and 0.0842 mg ai/L for frond area growth rate, in terms of mean 

measured concentrations. The % growth inhibition of frond number in the treated culture as compared to the negative 

control ranged from -7.8 to 90.4% after 7 days.

Fronds that grew together were observed in the 0.10, 0.31, 0.98, and 3.13 mg/L treatment groups. Slightly chlorotic 

fronds were observed in the 3.13 and 10 mg/L treatment groups. The pH increased from 7-7.5 (day 0) to 8.4-8.9 

(day 7) during the study.

This study is scientifically sound and is classified as Acceptable.

Results Synopsis

Frond number yield

IC05: 0.002691 mg ai/L 95% C.I.: N/A to 0.01311 mg ai/L

IC50: 0.05061 mg ai/L 95% C.I.: 0.0272 to 0.09415 mg ai/L

NOAEC: 0.03 mg ai/L

Probit Slope: N/A

Frond number growth rate

IC05: 0.00144 mg ai/L 95% C.I.: N/A to 0.008465 mg ai/L

IC50: 0.1089 mg ai/L 95% C.I.: 0.06598 to 0.1797 mg ai/L

NOAEC: 0.03 mg ai/L

Probit Slope: N/A

Frond area yield

IC05: 0.002842 mg ai/L 95% C.I.: N/A to 0.01232 mg ai/L

IC50: 0.04688 mg ai/L 95% C.I.: 0.02631 to 0.08352 mg ai/L

NOAEC: 0.03 mg ai/L

Probit Slope: N/A

Frond area growth rate

IC05: 0.001394 mg ai/L 95% C.I.: N/A to 0.00685 mg ai/L

IC50: 0.08417 mg ai/L 95% C.I.: 0.05415 to 0.1308 mg ai/L

NOAEC: 0.03 mg ai/L

Probit Slope: N/A

Endpoints affected: frond number yield, frond number growth rate, frond area yield, frond area growth rate

Page 2 of 12
Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic 

Vascular Plants (Lemna gibba G3)

MRID 49447201

I. MATERIALS AND METHODS

GUIDELINE FOLLOWED: The study was designed to comply with the procedures of the OECD 

Guideline 221 (2004). 

The following deviations from the U.S. EPA OCSPP 850.4400 (2012) guideline were noted:

1. The health of the inoculum culture was not reported. The inoculum should be from a logarithmically 

growing stock culture.

2. The pH, total organic carbon (TOC), particulate matter, metals, pesticides, and chlorine concentrations 

of the dilution water were not reported.

3. The number of plants/replicate and the number of fronds/plant were not reported; a total of 12 fronds 

per vessel were used.

4. Measured light intensity was 7360-10,000 lux; recommend light intensity is 4200-6700 lux.

5. Only 3 replicates were used per test level. A minimum of 4 replicates per level is recommended.

These deviations do not affect the validity of the study.

COMPLIANCE: Signed and dated GLP, Quality Assurance, and No Data Confidentiality 

statements were provided. The study was performed in accordance with 

OECD Principles of GLP, the Principles of GLP according to Annex 1 of 

the German chemical law (ChemG), USEPA 40 CFR Part 160, and JMAFF 

11 Nousan No. 6283.

A. MATERIALS

1. Test material Mesosulfuron (AE F154851)

Description White powder

Lot No./Batch No. LOR 21029

Purity 96.1%

Stability of compound 

under test conditions Stable. Day 7 measured concentrations ranged from 97 to 100% of their 

initial measured counterparts.

Storage conditions of 

test chemicals Stored at -20 ± 5°C.

Table 1. Physicochemical properties of Mesosulfuron

Parameter Values Comments

Water solubility at 20°C Not reported

Vapor pressure Not reported

UV absorption Not reported

Page 3 of 12
Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic 

Vascular Plants (Lemna gibba G3)

MRID 49447201

Parameter Values Comments

pKa Not reported

Kow Not reported

2. Test organism

Name: Duckweed, Lemna gibba

Strain: G3

Source: In-house cultures originally obtained from Dr. Janet Slovin, Horticulture 

Crops Quality Laboratory, U.S. Department of Agriculture, Beltsville, MD 

20705 U.S.A.

Age of inoculum: 7-10 days

Method of cultivation: Cultures were maintained in glass dishes filled with 20X AAP medium 

under illumination of 7360-10,000 lux and temperature of 24 ± 2°C for a 

minimum of three weeks. Transfers were made regularly into fresh medium 

to provide 7-10 days old colonies as test inoculum.

B. STUDY DESIGN

1. Experimental Conditions

a. Range-finding study

The range of test concentrations was selected based on pre experiments (data not reported).

b. Definitive study

Table 2. Experimental Parameters

Parameter Details Remarks

Criteria

Acclimation period

Culturing media and conditions

Health

Continuously cultured in-house

(maintained under test conditions at 

least three weeks)

Same as test

Not reported

Test system

Static/static renewal Static

Test concentrations were stable over 

the exposure period.

Static renewal intervals should be 

sufficient to maintain test 

concentrations.

Page 4 of 12
Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic 

Vascular Plants (Lemna gibba G3)

MRID 49447201

Parameter Details Remarks

Criteria

Incubation facility Growth incubator

Duration of the test 7 days

7 days 

Test vessel

Material (glass/stainless steel)

Size

Fill volume

Glass

470 mL

200 mL

Glass dishes (10 cm diameter, 6 cm 

in height) covered with glass lids.

Details of 20X AAP medium

pH in new solutions

pH in old solutions

Chelator used

Carbon source

7.0 to 7.5

8.4 to 8.9

Na2EDTA·2H2O

NaHCO3

EPA recommends the following 

culture media: 20X-AAP for Lemna 

gibba. Chelating agents (e.g. EDTA) 

are recommended in the nutrient 

medium for optimum cell growth. 

Lower concentrations of chelating 

agents (down to one-third of the 

normal concentration recommended 

for AAP medium) may be used in the 

nutrient medium used for test 

solution preparation if it is

suspected that the chelator will 

interact with the test material. 

ASTM reference, E1415-91and D 

3978-80 (reapproved 1987).

If non-standard nutrient medium 

was used, detailed composition 

provided.

A standard medium was used. A 

detailed composition was provided.

Dilution water 

Source/type

pH

Water pretreatment

Total organic carbon

Particulate matter

Metals

Pesticides

Chlorine

Ultra-pure water

Not reported

Purified (Milli-Q-water)

Not reported

Not reported

Not reported

Not reported

Not reported

pH of 7.5 ± 0.1 is acceptable if 20XAAP nutrient media is used.

Page 5 of 12
Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic 

Vascular Plants (Lemna gibba G3)

MRID 49447201

Parameter Details Remarks

Criteria

Indicate how the test material is 

added to the medium (added 

directly or used stock solution)

A stock solution was prepared by 

dissolving 104.5 mg of the test 

substance in 1000 mL of test 

medium. The resulting solution was 

stirred and sonicated, then 

transferred to a dilution series to 

obtain the concentration levels used 

in the study.

Aeration or agitation None

Sediment used (for rooted aquatic 

vascular plants)

Origin

Textural classification (%sand, silt, 

and clay)

Organic carbon (%)

Geographic location

N/A

Number of replicates

Negative control

Solvent control

Treatments

3

N/A

3 4 minimum

Number of plants/replicate Not reported

3-5 plants

Number of fronds/plant Not reported (total of 12 fronds per 

vessel)

3-4 fronds per plant 

Page 6 of 12
Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic 

Vascular Plants (Lemna gibba G3)

MRID 49447201

Parameter Details Remarks

Criteria

Test concentrations

Nominal

Mean measured*

0 (negative control) 0.003, 0.01, 

0.03, 0.10, 0.31, 0.98, 3.13, and 10.0

mg/L

<0.001349 (negative control) 0.003, 

0.01, 0.03, 0.10, 0.33, 1.03, 3.13, 

and 6.12 mg ai/L

*Mean measured concentrations 

were determined by the reviewer as 

the mean of Day 0 and Day 7 

measured concentrations.

Concentrations were not 

analytically-determined for the 

lowest two test levels (nominal 

0.003 and 0.01 mg/L). The reviewer 

estimated concentrations for the 

lowest two test levels based on the

average mean recovery for the other 

test levels (96.2% of nominal).

EPA requires at least 5 test 

concentrations except for limit tests.

Solvent Not used

Method and interval of analytical 

verification

Test concentrations were measured 

in newly prepared solutions on Day 

0 and from expired solutions on Day 

7 using HPLC-MS/MS.

Test conditions 

Temperature

Photoperiod

Light intensity and quality

23.7 to 24.0oC

Continuous

7630 to 10,000 lux (quality not 

reported)

Reference chemical Not used

Other parameters None

2. Observations

Table 3. Observation parameters

Parameters Details Remarks/Criteria

Parameters measured (e.g., number 

of fronds, plant dry weight or other 

toxicity symptoms)

Frond number

Frond area

Growth rate

Page 7 of 12
Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic 

Vascular Plants (Lemna gibba G3)

MRID 49447201

Parameters Details Remarks/Criteria

Measurement technique for frond 

number and other end points

Counting of fronds and 

determination of total frond area 

was carried out using a

LemnaTec Scanalyzer machine.

Observation intervals Fronds were counted on days 0, 

2, 5, and 7.

Other observations, if any Visual observations were made 

on study days 2, 5, and 7.

Indicate whether there was 

exponential growth in the control

Yes, the doubling time in the 

negative control was less than 2

days.

Were raw data included? Yes

II. RESULTS and DISCUSSION

A. INHIBITORY EFFECTS

After 7 days, the mean frond number of the negative control was 167 fronds, yielding inhibitions relative to the 

negative control of 0.0, 5.0, -7.8, 76.0, 87.0, 88.2, 89.4, and 90.4% for the mean measured concentrations of 

0.003, 0.01, 0.03, 0.10, 0.33, 1.03, 3.13, and 6.12 mg ai/L, respectively. The study author did not assess frond

number data.

The mean 0-7 day frond number growth rate of the negative control was 0.376/day, yielding inhibitions relative 

to the negative control of -0.1, 2.0, -2.9, 54.3, 77.7, 81.3, 85.7, and 89.2% for the mean measured concentrations

of 0.003, 0.01, 0.03, 0.10, 0.33, 1.03, 3.13, and 6.12 mg ai/L, respectively. The NOAEC and IC50 values 

reported by the study author based on frond number growth rate were 0.03 and 0.12 mg/L, respectively, in terms 

of nominal concentrations.

The mean 0-7 day frond number yield of the negative control was 155 fronds, yielding inhibitions relative to the 

negative control of 0.0, 5.4, -8.4, 81.9, 93.8, 95.1, 96.3, and 97.4% for the mean measured concentrations of 

0.003, 0.01, 0.03, 0.10, 0.33, 1.03, 3.13, and 6.12 mg ai/L, respectively. The study author did not assess frond

number yield data.

After 7 days, the mean frond area of the negative control was 1377 mm2

, yielding inhibitions relative to the 

negative control of -1.2, 8.2, -4.8, 77.7, 86.2, 87.3, 89.4, and 90.7% for the mean measured concentrations of 

0.003, 0.01, 0.03, 0.10, 0.33, 1.03, 3.13, and 6.12 mg ai/L, respectively. The study author did not assess frond

area data.

The mean 0-7 day frond area growth rate of the negative control was 0.367/day, yielding inhibitions relative to 

the negative control of 0.9, 3.1, 1.9, 60.9, 79.4, 85.5, 89.3, and 91.8% for mean measured concentrations of 

0.003, 0.01, 0.03, 0.10, 0.33, 1.03, 3.13, and 6.12 mg ai/L, respectively. The NOAEC and IC50 values reported 

by the study author based on frond area growth rate were 0.03 and 0.11 mg/L, respectively, in terms of nominal 

concentrations.

Page 8 of 12
Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic 

Vascular Plants (Lemna gibba G3)

MRID 49447201

The mean 0-7 day frond area yield of the negative control was 1271 mm2, yielding inhibitions relative to the 

negative control of -1.0, 8.8, -4.4, 84.7, 93.9, 95.8, 97.2, and 98.1% for the mean measured concentrations of 

0.003, 0.01, 0.03, 0.10, 0.33, 1.03, 3.13, and 6.12 mg ai/L, respectively. The study author did not calculate or 

assess frond area yield data.

Fronds that grew together were observed in the 0.10, 0.31, 0.98, and 3.13 mg/L treatment groups. Slightly 

chlorotic fronds were observed in the 3.13 and 10 mg/L treatment groups. The pH increased from 7-7.5 (day 0) 

to 8.4-8.9 (day 7) during the study.

Table 4. Effect of mesosulfuron on frond number of duckweed, Lemna gibba

Treatment

Mean measured 

and (nominal)

concentrations

mg ai/L

Initial frond 

number/test 

solution

frond number at

2 days 5 days 7 days

frond number % inhibition a

Negative control 12 28 83 167 N/A

0.003(0.003) 12 27 87 167 0.0

0.01(0.01) 12 24 81 159 5.0

0.03(0.03) 12 27 92 180 -7.8

0.10(0.10) 12 21 27 40 76.0

0.33(0.31) 12 18 21 22 87.0

1.03(0.98) 12 19 20 20 88.2

3.13(3.13) 12 15 18 18 89.4

6.12(10) 12 13 15 16 90.4

a Calculated by the reviewer relative to the negative control.

Table 5. Effect of mesosulfuron on frond number of duckweed, Lemna gibba

Treatment

Mean measured 

and (nominal) 

concentrations

mg ai/L

Initial frond 

number/test 

solution

Frond number growth rate

(day-1)

Frond number yield a

0-7 days % inhibition 0-7 days % inhibition b

Negative control 12 0.376 N/A 155 N/A

0.003(0.003) 12 0.376 -0.1 155 0.0

0.01(0.01) 12 0.368 2.0 147 5.4

0.03(0.03) 12 0.387 -2.9 168 -8.4

0.10(0.10) 12 0.172 54.3 28 81.9

0.33(0.31) 12 0.084 77.7 10 93.8

1.03(0.98) 12 0.070 81.3 8 95.1

3.13(3.13) 12 0.054 85.7 6 96.3

6.12(10) 12 0.041 89.2 4 97.4

a Calculated by the reviewer as final minus initial frond number b Calculated by the reviewer relative to the negative control.

Page 9 of 12
Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic 

Vascular Plants (Lemna gibba G3)

MRID 49447201

Table 6. Effect of mesosulfuron on biomass of duckweed, Lemna gibba

Treatment

Mean measured 

and (nominal) 

concentrations

mg ai/L

Frond area (mm2

) Frond area growth rate 

(day-1) Frond area yield a

Day 7 % inhibition b 0-7 days % inhibition 0-7 days % inhibition b

Negative control 1377 N/A 0.367 N/A 1271 N/A

0.003(0.003) 1393 -1.2 0.363 0.9 1284 -1.0

0.01(0.01) 1265 8.2 0.355 3.1 1160 8.8

0.03(0.03) 1443 -4.8 0.360 1.9 1327 -4.4

0.10(0.10) 307 77.7 0.143 60.9 194 84.7

0.33(0.31) 190 86.2 0.075 79.4 78 93.9

1.03(0.98) 175 87.3 0.053 85.5 54 95.8

3.13(3.13) 146 89.4 0.039 89.3 35 97.2

6.12(10) 128 90.7 0.030 91.8 25 98.1

a Calculated by the reviewer as the final minus initial frond area.

b Calculated by the reviewer relative to the negative control.

Table 7. Statistical endpoint values (calculated by the study author based on nominal concentrations)

Statistical 

Endpoint

Frond number Frond number 

growth rate

Frond area Frond area 

growth rate

NOAEC (mg/L) Not calculated 0.03 Not calculated 0.03

LOAEC (mg/L) Not calculated 0.10 Not calculated 0.10

IC50 or EC50

(mg/L) (95% C.I.)

Not calculated 0.12 (0.04-0.34) Not calculated 0.11 (0.006-0.23)

Reference chemical

NOAEC

IC50/EC50

N/A

N/A. Not applicable.

B. REPORTED STATISTICS

The study author statistically analyzed the endpoints for frond number growth rate and frond area growth rate using 

ToxRat Professional statistical software. The data were assessed for normality using the range-to-standarddeviation-ratio test and for homogeneity of variance using Cochran’s test or Welch t-test, respectively. The 

treatments were compared to the control using Dunnett’s Multiple t-test. The ECx values were determined using 

Probit analysis. All analyses were based on nominal concentrations.

Page 10 of 12
Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic 

Vascular Plants (Lemna gibba G3)

MRID 49447201

C. VERIFICATION OF STATISTICAL RESULTS

The reviewer assessed the endpoints for frond number yield, frond number growth rate, frond area yield, and 

frond area growth rate using CETIS version 1.8.7.12 statistical software using backend database settings 

implemented by EFED on 25 March 2014. Frond area growth rate and frond number growth rate data were 

confirmed to be normally distributed and have homogeneous variances using Shapiro-Wilk’s and Bartlett’s tests, 

respectively, and the data were therefore analyzed using Dunnett’s tests. Frond area yield data had non-normal 

distribution and unequal variances, and the data were therefore analyzed using Mann-Whitney tests. Frond 

number yield data were normally distributed, but had unequal variances and the data were therefore analyzed 

using Mann-Whitney tests. The ICx values were calculated using Bruce-Versteeg regression. All toxicity 

values are reported in terms of mean measured exposure concentrations.

Frond number yield

IC05: 0.002691 mg ai/L 95% C.I.: N/A to 0.01311 mg ai/L

IC50: 0.05061 mg ai/L 95% C.I.: 0.0272 to 0.09415 mg ai/L

NOAEC: 0.03 mg ai/L

Probit Slope: N/A

Frond number growth rate

IC05: 0.00144 mg ai/L 95% C.I.: N/A to 0.008465 mg ai/L

IC50: 0.1089 mg ai/L 95% C.I.: 0.06598 to 0.1797 mg ai/L

NOAEC: 0.03 mg ai/L

Probit Slope: N/A

Frond area yield

IC05: 0.002842 mg ai/L 95% C.I.: N/A to 0.01232 mg ai/L

IC50: 0.04688 mg ai/L 95% C.I.: 0.02631 to 0.08352 mg ai/L

NOAEC: 0.03 mg ai/L

Probit Slope: N/A

Frond area growth rate

IC05: 0.001394 mg ai/L 95% C.I.: N/A to 0.00685 mg ai/L

IC50: 0.08417 mg ai/L 95% C.I.: 0.05415 to 0.1308 mg ai/L

NOAEC: 0.03 mg ai/L

Probit Slope: N/A

D. STUDY DEFICIENCIES

See guideline deviations.

E. REVIEWER’S COMMENTS

The reviewer’s results were in agreement with those of the study author. The study author’s toxicity values were 

based on nominal concentrations whereas the reviewer’s values were based on mean measured concentrations. The 

study author did not analyze frond number yield or frond area yield data. The reviewer’s results are presented in the 

Executive Summary and Conclusions sections of this report.

The laboratory portion of the definitive test was conducted from 23 February to 02 March 2005.

The reviewer was unable to calculate time-weighted average (TWA) concentrations. Test concentrations were only 

Page 11 of 12
Data Evaluation Report on the Toxicity of AE F154851 (Mesosulfuron) to Aquatic 

Vascular Plants (Lemna gibba G3)

MRID 49447201

measured in newly prepared solutions on Day 0 and from expired solutions on Day 7.

The coefficient of variation (CV) of the negative control based on yield was 9.03-11.03% and based on growth rate 

was 3.16-3.18%. The guideline requirement for both yield and growth rate is < 20%.

F. CONCLUSIONS 

This study is scientifically sound and is classified as Acceptable.

Frond number yield

IC05: 0.002691 mg ai/L 95% C.I.: N/A to 0.01311 mg ai/L

IC50: 0.05061 mg ai/L 95% C.I.: 0.0272 to 0.09415 mg ai/L

NOAEC: 0.03 mg ai/L

Probit Slope: N/A

Frond number growth rate

IC05: 0.00144 mg ai/L 95% C.I.: N/A to 0.008465 mg ai/L

IC50: 0.1089 mg ai/L 95% C.I.: 0.06598 to 0.1797 mg ai/L

NOAEC: 0.03 mg ai/L

Probit Slope: N/A

Frond area yield

IC05: 0.002842 mg ai/L 95% C.I.: N/A to 0.01232 mg ai/L

IC50: 0.04688 mg ai/L 95% C.I.: 0.02631 to 0.08352 mg ai/L

NOAEC: 0.03 mg ai/L

Probit Slope: N/A

Frond area growth rate

IC05: 0.001394 mg ai/L 95% C.I.: N/A to 0.00685 mg ai/L

IC50: 0.08417 mg ai/L 95% C.I.: 0.05415 to 0.1308 mg ai/L

NOAEC: 0.03 mg ai/L

Probit Slope: N/A

Endpoints affected: frond number yield, frond number growth rate, frond area yield, frond area growth rate.

III. REFERENCES

Revised Proposal for a New Guideline OECD 221 “Lemna sp. Growth Inhibition Test” (April 2004).

Statistical Software “ToxRat Professional”, version 2.09, produced by ToxRat Solutions GmbH, 52477 Alsdorf, 

Germany (January 27, 2005).

ToxRat Validation Document from ToxRat Solutions GmbH, valid for ToxRat Version 2.09 (released January 25, 

2004).

M. Dorgerloh, “LemnaTec Scanalyzer Validation Program”, Bayer CropScience AG, unpublished report DOM 23038 of 

April 7, 2004.

Page 12 of 12
CETIS Summary Report Report Date: 27 Oct-14 19:19 (p 1 of 2)

Test Code: 122009 49447201 | 13-6193-9276

OCSPP 850.4400 Aquatic Vascular Plant Bayer CropScience AG

Batch ID: 04-2560-9572

Start Date: 23 Feb-05

Ending Date: 02 Mar-05

Test Type: Lemna Growth (7-d)

Duration: 7d 0h

Protocol: OCSPP 850.4400 Aquatic Plant (Lemna) Diluent: Lemna Medium, 20X-AAP

Species: Lemna Gibba Brine:

Source: Lab In-House Culture

Analyst:

Age: 7-10

Sample ID: 05-6897-8840

Sample Date: 23 Feb-05

Receive Date:

Code: 49447201

Sample Age: NA

Source: Bayer CropScience AG

Station:

Client: CDM Smith

Material: Mesosulfuron-methyl Project: Unknown

122009 49447201 static

122009 49447201 using mesosulfuron

Batch Note:

Sample Note:

Comparison Summary

Analysis ID Endpoint NOEL LOEL TOEL PMSD TU Method

11-9691-0524 Area Growth Rate 0.03 0.1 0.05477 4.68% Dunnett Multiple Comparison Test

16-1377-2374 Area Growth Rate 0.03 0.1 0.05477 3.41% Williams Multiple Comparison Test

13-0619-1884 Frond Area 0.1 0.33 0.1817 NA Jonckheere-Terpstra Step-Down Test

03-4998-1283 Frond Area 0.03 0.1 0.05477 11.6% Mann-Whitney U Two-Sample Test

16-6090-3627 Frond Number 0.1 0.33 0.1817 NA Jonckheere-Terpstra Step-Down Test

04-2583-3152 Frond Number 0.03 0.1 0.05477 9.81% Mann-Whitney U Two-Sample Test

20-6095-1587 Frond Number Growth Rate 0.03 0.1 0.05477 7.72% Dunnett Multiple Comparison Test

18-7448-1143 Frond Number Growth Rate 0.03 0.1 0.05477 5.62% Williams Multiple Comparison Test

95% LCL 95% UCL

Point Estimate Summary

Analysis ID Endpoint Level mg ai/L TU Method

03-6489-1028 Area Growth Rate IC5 0.001394 N/A 0.00685 Nonlinear Regression

IC10 0.003448 0.000266 0.009393

IC25 0.01566 0.007658 0.02847

IC50 0.08417 0.05415 0.1308

05-8526-2936 Frond Area IC5 0.002842 N/A 0.01232 Nonlinear Regression

IC10 0.005278 N/A 0.01459

IC25 0.01485 0.005427 0.03006

IC50 0.04688 0.02631 0.08352

07-6323-2088 Frond Number IC5 0.002691 N/A 0.01311 Nonlinear Regression

IC10 0.005145 N/A 0.01541

IC25 0.01519 0.005086 0.03238

IC50 0.05061 0.0272 0.09415

06-6140-0687 Frond Number Growth Rat IC5 0.00144 N/A 0.008465 Nonlinear Regression

IC10 0.003744 9.09E-05 0.01152

IC25 0.01848 0.008031 0.0366

IC50 0.1089 0.06598 0.1797

000-516-187-1 CETISTM v1.8.7.12 Analyst:________ QA:________
CETIS Summary Report Report Date: 27 Oct-14 19:19 (p 2 of 2)

Test Code: 122009 49447201 | 13-6193-9276

OCSPP 850.4400 Aquatic Vascular Plant Bayer CropScience AG

C-mg ai/L Control Type Count Mean Min Max Std Err Std Dev CV%

Area Growth Rate Summary

95% LCL 95% UCL %Effect

0 Negative Control 3 0.3663 0.3375 0.3951 0.354 0.377 0.006692 0.01159 3.16% 0.0%

0.003 3 0.3637 0.3557 0.3717 0.36 0.366 0.001856 0.003215 0.88% 0.73%

0.01 3 0.355 0.3101 0.3999 0.338 0.374 0.01044 0.01808 5.09% 3.09%

0.03 3 0.36 0.3445 0.3755 0.355 0.367 0.003606 0.006245 1.74% 1.73%

0.1 3 0.1433 0.1268 0.1599 0.136 0.149 0.003844 0.006658 4.65% 60.87%

0.33 3 0.07533 0.06908 0.08158 0.073 0.078 0.001453 0.002517 3.34% 79.44%

1.03 3 0.05267 0.04326 0.06207 0.05 0.057 0.002186 0.003786 7.19% 85.62%

3.13 3 0.03933 0.03554 0.04313 0.038 0.041 0.000882 0.001528 3.88% 89.26%

6.12 3 0.031 0.02017 0.04183 0.026 0.034 0.002517 0.004359 14.06% 91.54%

C-mg ai/L Control Type Count Mean Min Max Std Err Std Dev CV%

Frond Area Summary

95% LCL 95% UCL %Effect

0 Negative Control 3 1271 923 1620 1118 1393 80.95 140.2 11.03% 0.0%

0.003 3 1284 1206 1362 1248 1304 18.04 31.24 2.43% -1.0%

0.01 3 1160 680.1 1640 977 1362 111.5 193.2 16.66% 8.76%

0.03 3 1327 1130 1524 1236 1381 45.76 79.27 5.97% -4.38%

0.1 3 194.3 154.4 234.3 176 206 9.28 16.07 8.27% 84.71%

0.33 3 78 70.55 85.45 75 81 1.732 3 3.85% 93.86%

1.03 3 54 39.1 68.9 48 60 3.464 6 11.11% 95.75%

3.13 3 35.33 28.16 42.5 32 37 1.667 2.887 8.17% 97.22%

6.12 3 24.67 18.93 30.4 22 26 1.333 2.309 9.36% 98.06%

C-mg ai/L Control Type Count Mean Min Max Std Err Std Dev CV%

Frond Number Summary

95% LCL 95% UCL %Effect

0 Negative Control 3 155 120.2 189.8 139 165 8.083 14 9.03% 0.0%

0.003 3 155 141.2 168.8 149 160 3.215 5.568 3.59% 0.0%

0.01 3 146.7 104.4 188.9 130 164 9.821 17.01 11.6% 5.38%

0.03 3 168 137.9 198.1 161 182 7 12.12 7.22% -8.39%

0.1 3 28 23.03 32.97 26 30 1.155 2 7.14% 81.94%

0.33 3 9.667 4.496 14.84 8 12 1.202 2.082 21.53% 93.76%

1.03 3 7.667 3.872 11.46 6 9 0.8819 1.528 19.92% 95.05%

3.13 3 5.667 -1.922 13.26 3 9 1.764 3.055 53.91% 96.34%

6.12 3 4 -0.3027 8.303 2 5 1 1.732 43.3% 97.42%

C-mg ai/L Control Type Count Mean Min Max Std Err Std Dev CV%

Frond Number Growth Rate Summary

95% LCL 95% UCL %Effect

0 Negative Control 3 0.3757 0.346 0.4053 0.362 0.384 0.006888 0.01193 3.18% 0.0%

0.003 3 0.376 0.3646 0.3874 0.371 0.38 0.002646 0.004583 1.22% -0.09%

0.01 3 0.3683 0.3298 0.4068 0.353 0.384 0.00895 0.0155 4.21% 1.95%

0.03 3 0.3867 0.3623 0.411 0.381 0.398 0.005667 0.009815 2.54% -2.93%

0.1 3 0.172 0.1546 0.1894 0.165 0.179 0.004041 0.007 4.07% 54.21%

0.33 3 0.084 0.05058 0.1174 0.073 0.099 0.007767 0.01345 16.02% 77.64%

1.03 3 0.07033 0.04241 0.09825 0.058 0.08 0.006489 0.01124 15.98% 81.28%

3.13 3 0.054 -0.00624 0.1142 0.032 0.08 0.014 0.02425 44.91% 85.63%

6.12 3 0.04067 0.000509 0.08082 0.022 0.05 0.009333 0.01617 39.75% 89.17%

000-516-187-1 CETISTM v1.8.7.12 Analyst:________ QA:________