# Patent

## Title
Non-dehiscent sesame variety S28

## Abstract

A non-dehiscent sesame (Sesamum indicum L.) designated S28 has been found. Its degree of shatter resistance, or seed retention, makes S28 suitable for mechanized harvesting.

## Background
BACKGROUND
Sesame, or Sesamum indicum, is a tropical annual cultivated worldwide for its oil and its nut flavored seeds. In the Middle East, sesame is consumed as tahini, a sesame butter or sesame paste which is often mixed with ground chickpea kernels in a food preparation called hummus. Sesame is used widely in China, Japan, and Korea as a cooking oil, and it is consumed for its medicinal qualities. In recent years, the Japanese have been identifying and quantifying the medicinal benefits of sesame. In vitro studies and animal studies have indicated several antioxidant properties of sesame (Namiki, M. 1995. “The chemistry and physiological functions of sesame,” Food Rev Int 11:281–329). In the western hemisphere, sesame is primarily used in the confectionary trade in rolls and crackers. Throughout the world, sesame seeds or paste are mixed into sweets, e.g., halva. Sesame oil use in the cosmetic industry continues to expand.
The sesame plant grows to a height of about 56–249 cm, and at its leaf axils are found capsules which contain the sesame seed. Upon maturity in nature, the capsules holding the sesame seeds begin to dry down, the capsules normally split open, and the seeds fall out. Commercially, the harvester tries to recover as much seed as possible from mature capsules. From ancient times through the present, the opening of the capsule has been the major factor in attempting to successfully collect the seed. Harvesting methods, weather, and plant characteristics all contribute to the amount of seed recovered.
The majority of the world's sesame is harvested manually. With manual non-mechanized methods, it is desirable for the sesame seed to fall readily from the plant. Upon physiological maturity, the sesame stalks are cut, tied into small bundles, and then stacked in shocks. Further harvesting procedures vary from country to country and from area to area within countries. Some move the shocks to a threshing floor so that the seed that falls out can be recovered. Others put plastic or cloth in the fields under the shocks to catch the seed. For manual harvesting methods in which the dried, shocked sesame is moved to a threshing floor or over a plastic or cloth, preferred plant varieties include dehiscent, or super shattering, in which less than 10% of the seeds set are retained in the capsule.
Other methods involve leaving the shocks in the fields, and when the shocks are dry, the sesame is turned upside down and struck with an implement to shake out all of the seed. For this type of manual harvesting method, it is preferred that the capsule hold as much of the sesame seed as possible until the farmer inverts the stalk. Plant varieties rated as shattering which retain as much seed as possible before inversion are preferred. Common methods of manual harvest are discussed in Weiss, E. A. “Sesame”, Oilseed crops (2nd ed.), Chapter 5, Blackwell Science, Inc., Malden, Mass., p.131–164 (2000).
In an effort to mechanize the harvest of sesame, D. G. Langham introduced the use of binders in Venezuela in 1944. The binders were used to cut and bundle the sesame plants, manual labor was used to shock the cut plants, and combines were brought in to thrash the shocks. It was determined that seed shattering during mechanized harvesting methods caused considerable loss of sesame seed. While mechanization was considered to be essential for crop production in the Western hemisphere, it became obvious that the dehiscence of the sesame seed pod was the principal obstacle to the widespread acceptance of sesame as a commercial crop. (Langham, D. G. 1949. “Improvement of Sesame in Venezuela,” Proceedings First International Sesame Conference, Clemson Agricultural College, Clemson, S.C., pp. 74–79). As programs to introduce sesame production in the United States in Arizona, South Carolina, Nebraska, Oklahoma, and Texas were initiated, mechanization was considered essential due to high labor costs. Kalton, one of the Texas researchers, reported that the shattering nature of available strains was the main obstacle in complete mechanization of the sesame crop. (Kalton, R. 1949. “Sesame, a promising new oilseed crop for Texas,” Proc First International Sesame Conference, Clemson Agricultural College, Clemson, S.C., pp. 62–66).
In 1943, D. G. Langham found a mutation on a sesame plant. Capsules did not open on plants expressing this mutation. In succeeding generations, Langham showed that it was a recessive single gene which produced this indehiscence, where all the seeds were retained inside the unopened capsule. While it was believed that indehiscence would solve the problem of seed loss on mechanized harvesting, it was found that the capsules were too tough to effectively release the seed. Many of the capsules passed through a combine without opening. When more rigorous combining was attempted, an increase in efficiency of capsule opening was achieved but at the expense of seed quality. Seeds were broken due to the more rigorous combine conditions, and the broken seeds released free fatty acids. Chemical reactions with free fatty acids led to rancidity and concomitant undesirability of the harvested seed.
The indehiscent sesame varieties described above were used by various plant breeders in an attempt to develop desirable sesame lines. In addition to traditional cross-breeding approaches, some attempted to alter the chromosome numbers through tetraploids and interspecific crosses. Yermanos attempted to improve release of seed by increasing the length of the capsule so that there would be more surface for the combine to crack the capsules open (personal communication). Unfortunately, even with a small opening on the top of the capsule, a high percentage of broken seed was found on mechanized harvesting, preventing commercial use of this sesame line.
D. G. Langham reported in the late 1950's that the placenta attachment between each sesame seed and the placenta was important in the retention of seed in the capsule. He believed that he could improve the shatter resistance of sesame with increased placenta attachment but did not believe that all the seed could be retained in the capsule (Langham, D. G., Rodriguez, Maximo, and Reveron Esteban. 1956. “Dehiscencia y otras caracteristicas del ajonjoli, Sesamum indicum L., en relación con el problema de la cosecha”, Genesa, Maracay, Venezuela, pp. 3–16). However, Yermanos reported that during capsule maturity, the placenta attachment gradually weakens and is obliterated when the capsule is completely desiccated. (Yermanos, D. M. 1980. “Sesame. Hybridization of crop plants,” Am Soc Agronomy-Crop Sci of America, pp. 549–563). Thus, it appeared that the placenta attachment would have little effect on seed retention in dry, mature capsules during harvesting. A seamless gene which retained all the seed in the capsules was discovered by D. G. Langham and D. R. Langham in 1986. This was crossed with shattering types, and some progeny had an opening at the tip of the capsule. The seamless capsules were similar to the indehiscent capsules in that it was too difficult to remove the seed from the capsule without damaging the seed.
In 1982, the first non-shattering line (retaining 50–70% of the seeds set) requiring no manual labor was introduced. This line could be harvested by swathing the sesame, leaving it to dry in the field, and then picking it up by a combine. Although complete mechanization was achieved, extensive loss of seed due to adverse weather conditions continued to occur. (Langham, D. R., “Shatter resistance in sesame”, In: L. Van Zanten (ed.), Sesame improvements by induced mutations, Proc. Final FAO/IAEA Co-ord. Res. Mtng., IAEA, Vienna, TECDOC-1195, p.51–61 (2001)).
Other varieties were developed between 1988 and 1997 which allowed for direct combining with 70–90% seed retention, but extensive loss of seed due to wind and rain continued to occur. Lines that generally yielded 80% of the seed under ideal conditions would yield only 45–65% under adverse conditions. Thus, while many of the crosses began to moderate the deleterious effects of mechanized harvesting, none were able to increase the yields to the level of manually harvesting shattering cultivars.
U.S. Pat. No. 6,100,452 which issued Aug. 8, 2000, disclosed non-dehiscent sesame lines Sesaco 22 (S22), Sesaco 23 (S23), Sesaco 24 (S24), 19A, and 11W, representative seed having been deposited under ATCC accession number PTA-1400, PTA-1401, PTA-1402, PTA-1399, and PTA-1398, respectively. These sesame lines are characterized by their high degree of seed retention within the capsule despite adverse weather conditions such as wind and rain and the retention of a sufficient amount of sesame seed during mechanized harvesting to be competitive with manual harvesting with minimization of seed breakage.
U.S. patent application Ser. No. 10/135,855, filed Apr. 30, 2002, disclosed a non-dehiscent sesame cultivar S25, representative seed having been deposited under ATCC accession number PTA-4258. S25 is a stable, commercially suitable sesame line providing an early maturity cycle which extends the planting region to more northern latitudes and improved resistance against common fungal diseases.
U.S. patent application Ser. No. 10/189,800, filed Jul. 3, 2002, disclosed a non-dehiscent sesame cultivar S26, representative seed having been deposited under ATCC accession number PTA-4317. S26 is a stable, commercially suitable sesame line providing improved drought resistance, improved resistance against common fungal diseases, and a later maturity cycle which limits the planting region to more southern latitudes.
A non-dehiscent variety designated S28 has now been found which provides some improvements over previously disclosed non-dehiscent sesame lines.
SUMMARY OF THE INVENTION
In one aspect, the present invention is seed of sesame variety designated S28, a sample of the seed having been deposited under ATCC Accession No. PTA-6008.
In another aspect, the present invention is a sesame plant or parts thereof produced by growing the seed of sesame variety S28, a sample of the seed having been deposited under ATCC Accession No. PTA-6008. Pollen is one of the sesame plant parts of the present invention.
In another aspect, the present invention is a sesame plant having all the physiological and morphological characteristics of sesame variety S28, a sample of the seed of the variety having been deposited under ATCC Accession No. PTA-6008.
In another aspect, the present invention is a sesame plant having all the physiological and morphological characteristics of a sesame plant produced by growing seed of sesame variety S28, a sample of the seed having been deposited under ATCC Accession No. PTA-6008.
In another aspect, the present invention is a tissue culture of regenerable cells produced from seed of sesame variety S28, a sample of the seed having been deposited under ATCC Accession No. PTA-6008.
In another aspect, the present invention is a tissue culture of regenerable cells produced from sesame plant S28 or a part thereof produced by growing the seed of sesame variety S28, a sample of the seed having been deposited under ATCC Accession No. PTA-6008.
In another aspect, the present invention is a sesame plant regenerated from a tissue culture of regenerable cells produced from seed of sesame variety S28, a sample of the seed having been deposited under ATCC Accession No. PTA-6008, wherein the regenerated sesame plant has all the physiological and morphological characteristics of sesame variety S28.
In another aspect, the present invention is a sesame plant regenerated from a tissue culture of regenerable cells produced from a sesame plant produced by growing the seed of sesame variety S28, a sample of the seed having been deposited under ATCC Accession No. PTA-6008, wherein the regenerated sesame plant has all the physiological and morphological characteristics of sesame variety plant S28.
In another aspect, the present invention is a method of producing sesame seed, comprising crossing a first parent sesame plant with a second parent sesame plant, and harvesting the resultant sesame seed, wherein the first or second parent sesame plant was produced by growing seed of sesame variety S28, a sample of the seed having been deposited under ATCC Accession No. PTA-6008.



BRIEF DESCRIPTION OF DRAWINGS
FIG. 1 depicts the lineage of S28.
FIG. 2 depicts a comparison of the percent of seed retention during in shaker shatter resistance testing from 1997 to 2002 for sesame varieties released by Sesaco: Sesaco 01 (S01) released in 1982, Sesaco 02 (S02) released in 1983–1987, Sesaco 03 (S03) released in 1985–1986, Sesaco 04 (S04) released in 1984–1987, Sesaco 05 (S05) released in 1984, Sesaco 06 (S06) released in 1984, Sesaco 07 (S07) released in 1985–1988, Sesaco 08 (S08) released in 1986, Sesaco 09 (S09) released in 1985–1986, Sesaco 10 (S10) released in 1988–1991, Sesaco 11 (S11) released in 1988–1995, Sesaco 12 (S12) released in 1986–1987, Sesaco 14 (S14) released in 1989, Sesaco 15 (S15) released in 1990–1991, Sesaco 16 (S16) released in 1991–1996, Sesaco 17 (S17) released in 1993–1998, Sesaco 18 (S18) released in 1994–1996, Sesaco 19 (S19) released in 1994–1995, Sesaco 20 (S20) released in 1995–1997, Sesaco 21 (S21) released in 1995–1998, Sesaco 22 (S22) released in 1997–1998, Sesaco 23 (S23) released in 1998–2000, Sesaco 24 (S24) released in 1998–2004, Sesaco 25 (S25) released in 2001–2004, Sesaco 26 (S26) released in 2002–2004; and Sesaco 28 (S28) released in 2004.
FIG. 3 depicts a comparison of the kill resistance ratings in Uvalde, Tex., in 2002 for the varieties released by Sesaco.
FIG. 4 depicts a comparison of the mean days to physiological maturity from 2002 for the varieties released by Sesaco.
FIG. 5 depicts a comparison of the mean weight of 100 seeds in grams from 1997 to 2002 for the varieties released by Sesaco.



DETAILED DESCRIPTION
Sesame cultivar Sesaco 28 (hereinafter “S28”) is a non-dehiscent sesame variety having superior characteristics which make it a commercially suitable sesame line. S28 exhibits non-dehiscence as defined in U.S. Pat. No. 6,100,452, the teachings of which are incorporated herein by reference, making it suitable for mechanized harvesting. In addition, S28 has large seed size which is desirable for processors, comparable shatter resistance, earlier maturity which allows a wider geographical range, and improved resistance against common fungal diseases.
The recommended geographical area for S28 is from South Texas at the Rio Grande to northern Oklahoma and from east Texas westward to elevations below 1,000 meters. S28 has not been tested in other states, but it is projected that it would perform well in New Mexico, Arizona, and California. S28 has not been exposed to the sesame diseases that have been reported east of Texas.
Sesaco used the pedigree method of plant breeding to develop S28. Sesame is generally self-pollinated. Crossing is done using standard techniques as delineated in Yermanos, D. M. 1980. “Sesame. Hybridization of crop plants,” Am Soc Agronomy-Crop Sci of America, pp. 549–563 and U.S. Pat. No. 6,100,452. Ashri provides an overview of sesame breeding in Ashri, A. (1998). “Sesame breeding,” Plant Breed. Rev. 16:179–228. When Sesaco started the U.S. breeding program in 1978, there were no lines with all of the desirable characters for mechanization of sesame. Development of new varieties has depended on building blocks of desirable characters and then bringing in other blocks of desirable characters.
The lineage of S28 is presented in FIG. 1. 111 (1) was a line obtained from the National Plant Germplasm System (NPGS) (PI173955) in 1979 and first planted by Sesaco in the Woods nursery (Wellton, Ariz.) in 1981. NPGS obtained it in 1949 from W. N. Koelz, United States Department of Agriculture (USDA), Beltsville, Md. who obtained it from India. Within Sesaco, 111 first carried the identifier 0858 and was then changed to X111. In 1984, a selection of this line became Sesaco 4 (S04).
111X(2) was an outcross in the plot BT0458 in the Nickerson nursery (Yuma, Ariz.) in 1982. Within Sesaco, it carried the identifier E0745.
F820 (3) was a cross made by Sesaco in the Nickerson nursery (Yuma, Ariz.) in 1982. Within Sesaco, it carried the identifier F820.
104 (4) was a line obtained from the Sesamum Foundation (D. G. Langham, Fallbrook, Calif.) in 1977 and first planted in the Kamman nursery (Wellton, Ariz.) in 1978. It was obtained with the designator SF084. The Sesamum Foundation obtained it from Maximo Rodriguez in 1961. He had collected it from Mexico where it was known as Instituto 8. Instituto 8 was a selection from G53.48, a cross made by D. G. Langham in 1953 in Guacara, Venezuela. Within Sesaco, 104 carried the identifier 0084. In 1983, a selection of this line became Sesaco 2 (S02).
578 (5) was a cross made by Sesaco in the McElhaney nursery (Wellton, Ariz.) in 1983. Within Sesaco, it carried the identifier G8578 and then changed to T578.
F853 (6) was a cross made by Sesaco in the Nickerson nursery (Yuma, Ariz.) in 1982. Within Sesaco, it carried the identifier F853.
192 (7) was a line obtained from the M. L. Kinman in 1980 and first planted by Sesaco in the Woods nursery (Wellton, Ariz.) in 1981. The line was originally T61429-B-4-1-3 from the Kinman USDA sesame program, College Station, Tex., which had been in cold storage at Ft. Collins, Colo. In 1997, the line was transferred to the NPGS, Griffin, Georgia and given the identifier PI599462. Within Sesaco, 192 first carried the identifier 1479 and then was changed to X191 and X193. In 1985, a selection from X193 became Sesaco 3 (S03) and a selection of X191 became Sesaco 7 (S07).
031 (8) was a cross made by Sesaco in the Ramsey nursery (Roll, Ariz.) in 1984. Within Sesaco, it carried the identifier H0031 and then changed to T031.
118 (9) was a line obtained from the NGPS (PI425944) in 1979 and first planted in Kamman nursery (Wellton, Ariz.) in 1979. The NGPS obtained it in 1978 from P. F. Knowles, University of California, Davis, Calif., who collected it in Pakistan. Within Sesaco, it carried the identifier 1118 and then changed to X118 and then to T118.
BI954 (10) was a cross made by Sesaco in the Gilleland nursery (Uvalde, Tex.) in 1993. Within Sesaco, it carried the identifier B1954.
72C (11) was a line obtained from the NGPS (PI292146) in 1979 and first planted in Woods nursery (Wellton, Ariz.) in 1981. The NGPS obtained it in 1963 from Hybritech Seed International, a unit of Monsanto, U.S., which obtained it from Israel. In viewing this material in 1986, A. Ashri of Israel concluded that it was an introduction to Israel. The material is similar to introductions from the Indian subcontinent. Within Sesaco, it has carried the identifier 0702 and then changed to X702 and then to X702C. In 1986, a selection from X702C became Sesaco 12 (S12).
L6651 (12) was a cross made by Sesaco in the Wright nursery (Roll, Ariz.) in 1987. Within Sesaco, it carried the identifier L6651.
G8 (13) was a line obtained from D. G. Langham in 1977 and first planted by Sesaco in the Kamman nursery (Wellton, Ariz.) in 1978. It was a selection from the cultivar ‘Guacara’ which D. G. Langham developed in Venezuela in the 1950s. Guacara was an initial selection from a cross that later produced one of the major varieties in Venezuela-Aceitera. Within Sesaco, G8 first carried the identifier X011 and was later changed to TG8.
804 (14) was a cross made by Sesaco in the Nickerson nursery (Yuma, Ariz.) in 1982. Within Sesaco, it has carried the identifier F804; in 1988, a selection of this line became Sesaco 11 (S11).
2CA (15) was a cross made by Sesaco in the Wright nursery (Roll, Ariz.) in 1988. Within Sesaco, it has carried the identifier LCX02 and later changed to X2CA and then to T2CA.
S11(16) was a cross made by Sesaco in the Nickerson nursery (Yuma, Ariz.) in 1982. Within Sesaco, it carried the identifier F804. In 1988, a selection of this line became Sesaco 11 (S11).
SOMALIA (17) was a line obtained from the NGPS (PI210687) in 1979 and first planted in Kamman nursery (Wellton, Ariz.) in 1979. The NGPS obtained it from the Administrazione Fiduciaria Italiana della Somalia, Mogadishu, Somalia. Within Sesaco, it carried the identifier 0730.
H6778 (18) was a cross made by Sesaco in the Hancock nursery (Wellton, Ariz.) in 1984. Within Sesaco, it carried the identifier H6778.
J3208 (19) was a cross made by Sesaco in the Hancock nursery (Wellton, Ariz.) in 1985. Within Sesaco, it carried the identifier J3208.
193 (20) was a selection from 192 (7) described above. Within Sesaco, it carried the identifier X193. In 1985, a selection of X193 became Sesaco 03 (S03).
H6432 (21) was a cross made by Sesaco in the Hancock nursery (Wellton, Ariz.) in 1984. Within Sesaco, it carried the identifier H6432.
MAX (22) was a line obtained from the Sesamum Foundation (D. G. Langham, Fallbrook, Calif.) in 1977 and first planted in the Kamman nursery (Wellton, Ariz.) in 1978. The Sesamum Foundation obtained it from Maximo Rodriguez in 1961. He had collected it from Mexico where it was known as Instituto Regional Canasta. Within Sesaco, it carried the identifier 0116 and then changed to TMAX.
076 (23) was a cross made by Sesaco in the Kamman nursery (Wellton, Ariz.) in 1979. Within Sesaco, it carried the identifier C076 and then changed to T076.
R234 (24) was a named variety obtained from D. M. Yermanos in 1978 from his sesame program at the University of California at Riverside. It was first planted in the Kamman nursery (Wellton, Ariz.) in 1978. Within Sesaco, it carried the identifier 0544 and then changed to T234.
R234 TALL (25) was an outcross found in the Kamman nursery (Wellton, Ariz.) in 1979. Within Sesaco, it carried the identifier X026.
K3255 (26) was a cross made by Sesaco in the Hancock nursery (Wellton, Ariz.) in 1986. Within Sesaco, it carried the identifier K3255.
045 (27) was a cross made by Sesaco in the Kamman nursery (Wellton, Ariz.) in 1978. Within Sesaco, it carried the identifier B045 and then changed to T045.
G958-1 (28) was a line obtained from the Sesamum Foundation in 1977 and first planted in the Kamman nursery (Wellton, Ariz.) in 1978. It was obtained with a designator of SF411. The Sesamum Foundation obtained it from John Martin in 1962 who had obtained it from the D. G. Langham breeding program in Venezuela. Within Sesaco, G958-1 carried the identifier 0411.
H6785 (29) was a cross made by Sesaco in the Hancock nursery (Wellton, Ariz.) in 1984. Within Sesaco, it carried the identifier H6785.
982 (30) was a line obtained from the Sesamum Foundation in 1977 and first planted in the Kamman nursery (Wellton, Ariz.) in 1978. It was obtained with a designator of SF477 and was named G53.98-2. The Sesamum Foundation obtained it from John Martin in 1962 who had obtained it from the D. G. Langham breeding program in Venezuela. G53.98-2 was a cross made by D. G. Langham in 1953 in Guacara, Venezuela. Within Sesaco, 982 carried the identifier 0477 and then changed to T982.
036 (31) was a cross made by Sesaco in the Kamman nursery (Wellton, Ariz.) in 1979. Within Sesaco, it carried the identifier C036 and then X036. In 1984, a selection from X036 became Sesaco 6 (S06).
G53.80-1 (32) was a line obtained from the Sesamum Foundation in 1977 and first planted in the Kamman nursery (Wellton, Ariz.) in 1978. It was obtained with a designator of SF471. The Sesamum Foundation obtained it from John Martin in 1962 who had obtained it from the D. G. Langham breeding program in Venezuela. G53.80-1 was a cross made by D. G. Langham in 1953 in Guacara, Venezuela. Within Sesaco, G53.80-1 carried the identifier 0471.
J3222 (33) was a cross made by Sesaco in the Hancock nursery (Wellton, Ariz.) in 1985. Within Sesaco, it carried the identifier J3222.
195 (34) was an outcross selected in plot MN4584 in the McElhaney nursery (Wellton, Ariz.) in 1983. Within Sesaco, it carried the identifier E0690 and then changed to X195.
H6562 (35) was a cross made by Sesaco in the Hancock nursery (Wellton, Ariz.) in 1984. Within Sesaco, it carried the identifier H6562.
701 (36) was a line obtained from the NGPS (PI292145) in 1979 and first planted in Woods nursery (Wellton, Ariz.) in 1981. The NGPS obtained it in 1963 from Hybritech Seed International, a unit of Monsanto, U.S., which obtained it from Israel. In viewing this material in 1986, A. Ashri of Israel concluded that it was an introduction to Israel. The material is similar to introductions from the Indian subcontinent. Within Sesaco, it carried the identifier 0701 and then changed to X701. In 1984, a selection from X701 became Sesaco 5 (S05).
S16 (37) was a cross made by Sesaco in the Wright nursery (Roll, Ariz.) in 1987. Within Sesaco, it carried the identifier KAP11 and then changed to XFXA. In 1991, a selection from XFXA became Sesaco 16 (S16).
S28 (38) was a cross made by Sesaco in the Friesenhahn nursery (Knippa, Tex.) in 1994. The original designator was CM764. The seed (M764) from the cross was planted in plot E082 in the Friesenhahn nursery (Knippa, Tex.) in 1995. Four plants were selected based on “031 genes, a good phenotype, and good kill resistance.” The seed (2717) from one of the plants was planted in plot 2067 in the Gilleland nursery (Uvalde, Tex.) in 1996. Six plants were selected based on “good low height of the first capsule and producing a wide row.” The seed (1375) from one of the plants was planted in plot 4677 in the Gilleland nursery (Uvalde, Tex.) in 1997. Nineteen plants were selected based on “good kill resistance, wide row, lot of capsules in the row, light seeking ability, and bottom regrowth.” The identifier was changed to X13J. The seed (3240) from one of the plants was planted in plot 0213 in the Gilleland nursery (Uvalde, Tex.) in 1998. Five plants were selected based on “good branches, lot of capsules, wide row, and capability of growing on 101 cm rows.” The seed (2838) from one of the plants was planted in plot 8211 in the Schwartz nursery (Wall, Tex.) in 1999. A bulk of 24 plants was selected based on “wide row, good for 40” spacing, excellent drought resistance, seed to the top of the plant.” The bulk seed (4719) was planted in plot WK37 in the Schwartz nursery (Wall, Tex.) in 2000. A ten capsule sample was taken to test for non-dehiscence and the results were 75% seed retention. The notes in plot WK37 said, “strip OK, weather shatter resistance.” The 10 capsule sample (R0163) was planted in plot 0150 in the Gilleland nursery (Uvalde, Tex.) in 2001. A bulk of 69 plants was taken based on “earlier, very uniform, wide row, lower, good internode length, no kill.” The bulk seed (Y245) was planted in strip WHOO in the Gilleland nursery (Uvalde, Tex.) in 2002. The strip was combined. The combined seed was tested in two farms: the Schwartz farm (Wall, Tex.) and the W. Gilleland farm (Uvalde, Tex.) in 2003, and the seed combined. The combined seed was released to farmers in May, 2004.
Along with breeding programs, tissue culture of sesame is currently being practiced in Korea, Japan, Sri Lanka and the United States. It is possible for one of ordinary skill in the art to utilize sesame plants grown from tissue culture as parental lines in the production of non-dehiscent sesame. Further, it is possible to propagate non-dehiscent sesame through tissue culture methods. By means well known in the art, sesame plants can be regenerated from tissue culture having all the physiological and morphological characteristics of the source plant.
The present invention includes the seed of sesame variety S28 deposited under ATCC Accession No. PTA-6008; a sesame plant or parts thereof produced by growing the seed deposited under ATCC Accession No. PTA-6008; any sesame plant having all the physiological and morphological characteristics of sesame variety S28; any sesame plant all the physiological and morphological characteristics of a sesame plant produced by growing the seed deposited under ATCC Accession No. PTA-6008. The present invention also includes a tissue culture of regenerable cells produced from the seed having been deposited under ATCC Accession No. PTA-6008 or a tissue culture of regenerable cells from sesame variety S28 or a part thereof produced by growing the seed of sesame variety S28 having been deposited under ATCC Accession No. PTA-6008. A sesame plant regenerated from a tissue culture of regenerable cells produced from the seed having been deposited under ATCC Accession No. PTA-6008 or from sesame variety S28, wherein the regenerated sesame plant has all the physiological and morphological characteristics of sesame variety S28 is also contemplated by the present invention. Methods of producing sesame seed, comprising crossing a first parent sesame plant with a second parent sesame plant, wherein the first or second parent sesame plant was produced by seed having been deposited under ATCC Patent Deposit Designation No. PTA-6008 are part of the present invention.
Unless otherwise stated, as used herein, the term plant includes plant cells, plant protoplasts, plant cell tissue cultures from which sesame plants can be regenerated, plant calli, plant clumps, plant cells that are intact in plants, or parts of plants, such as embryos, pollen, ovules, flowers, capsules, stems, leaves, seeds, roots, root tips, and the like. Further, unless otherwise stated, as used herein, the term progeny includes plants derived from plant cells, plant protoplasts, plant cell tissue cultures from which sesame plants can be regenerated, plant calli, plant clumps, plant cells that are intact in plants, or parts of plants, such as embryos, pollen, ovules, flowers, capsules, stems, leaves, seeds, roots, root tips, and the like.
Sesame cultivar S28 has been extensively grown in the following two nurseries:
(1) Uvalde nursery planted north of Uvalde, Tex. (latitude 29°22′ north, longitude 99°47′ west, 226 m elev) in middle to late May from 1988–2001; mean rainfall is 608 mm annually with a mean of 253 mm during the growing season;
temperatures range from an average low of 3° C. and an average high of 17° C. in January to an average low of 22° C. and an average high of 37° C. in July. The nursery was planted on 96 cm beds from 1988 to 1997 and on 76 cm beds from 1998 to 2001. The nursery was pre-irrigated and has had 2–3 post-plant irrigations depending on rainfall. The fertility has varied from 30–60 units of nitrogen. There is no data for 2003 because the Uvalde nursery was first hit by a hail storm, and then the eye of hurricane Claudette passed over the nursery with sustained winds over 80 kph. The data is not considered typical based on 16 years of data in that area; and
(2) San Angelo nursery planted east of San Angelo, Tex. (latitude 31°21′ north, longitude 100°30′ west, 908 m elev) in early to middle June from 1992–2001; mean rainfall is 490 mm annually with a mean of 201 mm during the growing season; temperatures range from an average low of 0° C. and an average high of 15° C. in January to an average low of 22° C. and an average high of 36° C. in July. The nursery was planted on 101 cm beds. The nursery was pre-irrigated in years when there was no planting rain and has had 0 to 1 post-plant irrigations depending on rainfall. The fertility has varied from 20–50 units of nitrogen. There is no data for 2003 because the sample sizes were too low to be considered valid.
Sesame cultivar S28 has shown uniformity and stability within the limits of environmental influence for the characters listed in Table I below. Table I provides the name, definition, and rating scale of each character as well as the method by which the character is measured. Under the rating section, the rating for S28 is presented in bold text. Additionally, the distribution of the character in Sesaco's sesame development program is indicated under the rating section. Sesaco uses slightly different character specifications from “Descriptors for sesame”, AGP:IBPGR/80/71, IBPGR Secretariat, Rome, (1981) and from the form “Sesame (Sesamum indicum)”, U.S. Department of Agriculture Plant Variety Protection Office, Beltsville, Md. The descriptors in those documents were developed in the early 1980s and have not been updated to incorporate new concepts in sesame data collection.
Regarding the data given in Table I, the averages and distributions within Sesaco varieties of most characters have not changed from previous reports because there has been little change. The values for leaf measurements has been updated because of more extensive testing in 2002, with current data based on 711 samples of 196 lines compared to previous reports on 100 fifth, 100 tenth, and 59 fifteenth leaf measurements. In addition, the data from light sensitive lines from the tropics have been omitted because none of these extremely large leaf materials can be used commercially in the U.S. In addition, the capsule data has also been updated since current data is based on 8,285 samples of 1,613 lines versus previous reports with 3,144 samples.







TABLE I







Characters Distinguishing the S28 Line









Character
Rating
Methodology





(1) BRANCHING
Subjective rating
The amount of branching on any particular


STYLE
Values:
plant depends on the space around the plant. In


The potential amount of
U = Uniculm - no branching
high populations, branching can be suppressed.


true branching in a line
except weak branches in
This rating should be based on potential as



open
expressed on end plants and plants in the open.



B = True branches
True branches start in the leaf axil below



S28 = B for all crops in all
the first flower, and they begin to emerge



nurseries
before the first open flower. As long as there is



Distribution within Sesaco
light into the leaf axils, there will be additional



based on stable lines in the
branches that start below the first branches in



crossing program in 1982–2001
subsequently lower nodes. Weak branches



(Total number of
occur when a plant is in the open. They



samples tested = 1,333)
develop in the lowest nodes and subsequent



U = 42.4%
branches start at higher nodes. There are lines



B = 57.6%
that will not branch in any circumstance.




Some lines in the open will put on




spontaneous branches late in the cycle. True




and weak branches do not have a capsule in the




same leaf axil, whereas the spontaneous




branches form under the capsule after the




capsule has formed. Spontaneous branches are




not counted as branches.




There are rare lines where the flowering




pattern is to put on flowers on lower nodes late




in the cycle. In this case, the capsule is formed




after the branch is developed. This pattern




should not be termed spontaneous branching,




and the branch is normally counted as a true




branch.




There are branched lines that have




secondary branches on the branches. In a few




cases, there can be tertiary branches.




Additional branches generally appear in low




populations.




Comments: the effects of light appear to




have more of an effect on branching than




moisture and fertility. High populations




suppress branching.


(2) NUMBER OF
Subjective rating
Rating can be taken from about 60 days


CAPSULES PER
Values:
after planting through to the end of the crop.


LEAF AXIL
1 = Single
Number of Capsules per Leaf Axil is highly


The predominant number
3 = Triple
dependent on moisture, fertility, and light. In


of capsules per leaf axil in
Based on potential as
triple capsule lines, the central capsule forms


the middle half of the
described in the methodology
first, and axillary capsules follow a few days


capsule zone
presented herein
later. Triple capsule lines have the potential to



S28 = 1 for all crops in all
put on axillaries, but will not do so if plants do



nurseries
not have adequate moisture and/or fertility. In



Distribution within Sesaco
drought conditions, some triple capsule lines



based on stable lines in the
will produce only a central capsule for many



crossing program in 1982–2001
nodes. In these lines, when there is adequate



(Total number of
moisture through rain or irrigation, some will



samples tested = 1,327)
add axillary capsules on only new nodes, while



1 = 58.3%
others will add axillary capsules to all nodes.



3 = 41.7%
Some triple capsule lines will not put on




axillary capsules if there is no direct sunlight




on the leaf axil. To date, lines with single




capsules have nectaries next to the central




capsule in the middle of the capsule zone while




triple capsules do not. However, some lines




have what appear to be nectaries on the lower




capsules of triple lines, but upon close




examination, they are buds which may or may




not eventually develop into a flower and then a




capsule. In most triple capsule lines, the lower




and upper nodes have single capsules.




There are some lines where the end plants




can put on 5 capsules/leaf axil and a few that




have the potential to put on 7 capsules/leaf axil.




5 and 7 capsules only appear with open plants




with high moisture and fertility. In some




environments, single capsule lines will put on




multiple capsules on 1 node and rarely on up to




5 nodes. These lines are not considered triple




capsule lines.


(3) MATURITY CLASS
Subjective rating
The basis for this data point is Days to


The maturity of a line in
Values:
Physiological Maturity (Character No. 27). S24


relation to a standard line.
V = Very early (<85 days)
is the standard line to be used to compute


Currently, the standard
E = Early (85–94 days)
Maturity Class. In 1998–2001, the maturity of


line is S24 at 95 days
M = Medium (95–104 days)
S24 averaged 95 days in the Uvalde, TX,



L = Late (105–114 days)
nursery. For each line, the physiological



T = Very late (>114 days)
maturity for each year is subtracted by the S24



S28 = M in 2002 in Uvalde
maturity for that year in that nursery, and then



nurserya
the number of days of difference is averaged.



Distribution within Sesaco
The average is then added to 95.



based on stable lines in the
See Days to Physiological Maturity



crossing program in 1998–2001
(Character No. 27) for the effects of the



(Total number of
environment on Maturity Class.



samples tested = 650)



V = 1.2%



E = 26.8%



M = 56.2%



L = 12.9%



T = 2.9%


(4) PLANT
Subjective rating
The first character is the Branching Style


PHENOTYPE
Values:
(Character No. 1), followed by the Number of


A three character
Branching Style
Capsules per Leaf Axil (Character No. 2), and


designation that provides
U = Uniculm - no branching
then the Maturity Class (Character No. 3).


the branching style,
except weak branches in
When these characters are placed in a


number of capsules per
open
matrix, there are 20 potential phenotypes. The


leaf axil, and the maturity
B = True branches
phenotype provides an overview of the general


class
Number of Capsules per Leaf
appearance of the plant. There is a very high



Axil
correlation between Maturity Class and Height



1 = Single
of Plant (Character No. 5).



3 = Triple



Maturity Class



V = Very early (<85 days)



E = Early (85–94 days)



M = Medium (95–104 days)



L = Late (105–114 days)



T = Very late (>114 days)



S28 = B1M for all crops in



all nurseries



Distribution within Sesaco



based on stable lines in the



crossing program in 1998–2001



(Total number of



samples tested = 650)










U1V = 0%
U3V = 1.1%



U1E = 3.8%
U3E = 8.3%



U1M = 16.0%
U3M = 12.0%



U1L = 3.4%
U3L = 2.2%



U1T = 0.5%
U3T = 0.6%



BIV = 0%
B3V = 0.2%



B1E = 8.0%
B3E = 6.3%



B1M = 23.2%
B3M = 4.8%



B1L = 6.5%
B3L = 1.0%



B1T = 1.6%
B3T = 0.4%









(5) HEIGHT OF
Unit of measure: cm
The measurement is made after the plants


PLANT
Values: average of 3 plants
stop flowering. For plants that are not erect or


The height of the plant
S28 = 149 cm in 2002 in
have lodged, the plant should be picked up for


from the ground to the top
Uvalde nursery
the measurement. In most lines the highest


of the highest capsule
Distribution within Sesaco
capsule is on the main stem. In lines with the


with viable seed
based on stable lines in the
dt/dt alleles (determinate), the highest capsule



crossing program in 1999–2001
is on the branches.



(Total number of
Comments: this height is dependent on the



samples tested = 2274)
amount of moisture, heat, fertility, and



low = 56 cm; high = 249 cm
population. Increased values generally increase



1 = <94.6 cm; 5.2%
the height. In a high population, the height will



2 = <133.2 cm; 34.6%
only increase if there is adequate fertility and



3 = <171.8 cm; 54.9%
moisture; otherwise, the height will be shorter.



4 = <210.4 cm; 5.1%
In low light intensities, the heights are



5 = >210.3 cm; 0.1%
generally taller.



avg. = 134.8 cm, std = 23.5


(6) HEIGHT OF FIRST
Unit of measure: cm
The measurement is made after the plants


CAPSULE
Values: average of 3 plants
stop flowering. For plants that are not erect or


The height of the first
S28 = 58 cm in 2002 in
have lodged, the plant should be picked up for


capsule from the ground
Uvalde nursery
the measurement. In most lines, the lowest


to the bottom of the
Distribution within Sesaco
capsule is on the main stem. True branches


lowest capsule on the
based on stable lines in the
have capsules higher than on the main stem


central stem
crossing program in 1999–2001
except when the flowers fall off the main stem.



(Total number of
Occasionally, on weak branches, the lowest



samples tested = 2274)
capsule is on the branches.



low = 20 cm; high = 193 cm
There are lines that flower in the lower



1 = <54.6 cm; 52.7%
nodes late in the cycle, and, thus, the measurement



2 = <89.2 cm; 45.5%
should be taken after flowering ends. In



3 = <123.8 cm; 1.5%
many lines the first flower does not make a



4 = <158.4 cm; 0.3%
capsule, and, thus, this height may not be the



5 = >158.3 cm; 0.1%
same as the height of the first flower. The



avg. = 54.2 cm, std = 14.3
height is correlated to the length of time to




flowering, the earlier the lower the height.




Comments: see Height of Plant (Character




No. 5) for effects of environmental factors


(7) CAPSULE ZONE
Unit of measure: cm
The measurement is derived by subtracting


LENGTH
Values: average of 3 plants
the Height of First Capsule (Character No. 6)


The length of the capsule
S28 = 91 cm in 2002 in
from the Height of Plant (Character No. 5).


zone. The capsule zone
Uvalde nursery
Comments: see Height of Plant (Character


extends from the bottom
Distribution within Sesaco
No. 5) for effects of environmental factors


of the lowest capsule on
based on stable lines in the


the main stem to the top
crossing program in 1999–2001


of the highest capsule on
(Total number of


the main stem.
samples tested = 2274)



low = 18 cm; high = 188 cm



1 = <52 cm; 4.7%



2 = <86 cm; 53.5%



3 = <120 cm, 41.3%



4 = <154 cm; 0.5%



5 = >153.9 cm; 0.1%



avg. = 80.6 cm, std = 17.2


(8) NUMBER OF
Unit of measure: number
The count is made after the plants stop


CAPSULE NODES
Values: average of 3 plants
flowering. On opposite and alternate arranged


The number of capsule
S28 = 23 in 2002 in Uvalde
leaves, each pair of leaves is counted as one


nodes from the lowest
nursery
node. In some lines, there are three leaves per


capsule node to the
Distribution within Sesaco
node for at least part of the plant. In some


highest node with
based on stable lines in the
plants, flowers may not have produced


capsules with viable seed
crossing program in 1999–2001
capsules on one or more of the leaf axils in a


on the main stem of the
(Total number of
node. These nodes should still be counted.


plant
samples tested = 2154)
Nodes on the branches are not counted.



low = 10; high = 54
In years when the amount of moisture



1 = <18.8; 17.9%
available to the plant is irregular, nodes can



2 = <27.6; 48.3%
become very irregular, particularly on triple



3 = <36.4; 29.5%
capsule lines. In the upper portions of the plant,



4 = <45.2; 3.6%
it may become easier to count the capsule



5 = >45.1; 0.7%
clusters and divide by 2. While it is possible to



avg. = 25.3, std = 6.4
count nodes after leaves have fallen, it is much




easier to count while the leaves are still on the




plant.




Comments: the number of nodes is




dependent on the amount of moisture and




fertility. Higher moisture and fertility increases




the number of nodes.


(9) AVERAGE
Unit of measure: cm
Divide the Capsule Zone Length (Character


INTERNODE
Values: average of 3 plants
No. 7) by the Number of Capsule Nodes


LENGTH WITHIN
S28 = 4.0 cm in 2002 in
(Character No. 8).


CAPSULE ZONE
Uvalde nursery
Comments: this length is dependent on the


The average internode
Distribution within Sesaco
amount of moisture, fertility, and population.


length within the capsule
based on stable lines in the
Increased values generally increase the length.


zone
crossing program in 1999–2001
In a high population, the length will only



(Total number of
increase if there is adequate fertility and



samples tested = 2145)
moisture; otherwise the length will be shorter.



low = 1.09 cm; high = 8.09 cm
In low light intensities, the lengths are



1 = <2.49 cm; 6.2%
generally longer.



2 = <3.89 cm; 74.6%
Past methodologies have measured the



3 = <5.29 cm; 18.6%
internode length at the middle of the capsule



4 = <6.69 cm; 0.4%
zone. Some have measured it at the median



5 = >6.68 cm; 0.1%
node and others at the median Capsule Zone



avg. = 3.35 cm, std = 0.66
Length.


(10) YIELD AT
Unit of measure: kg/ha
On 3 replicated plots, when the plants are


DRYDOWN
Values: average of 3
dry enough for direct harvest, cut a minimum


An extrapolation of the
replications
of 1/5000 of a hectare (Sesaco uses 1/2620) in


yield of a field by taking
S28 = 1624 kg/ha in 2002 in
the plot and place the plants in a cloth bag.


sample yields
Uvalde nursery; 1292 kg/ha
Thresh the sample in a plot thresher and weigh



in 2002 in San Angelo
the seed. Multiply the weight by the



nurseryb
appropriate multiplier based on area taken to



Distribution within Sesaco
provide the extrapolated yield in kg/ha.



based on stable lines in the
In the Almaco thresher there is about 3%



crossing program in 1999–2001
trash left in the seed. Since yields are comparative,



(Total number of
there is no cleaning of the seed done



samples tested = 1828)
before the computation. If other threshers have



low = 67 kg/ha
more trash, the seed should be cleaned before



high = 2421 kg/ha
weighing.



1 = <537.8 kg/ha; 5.6%
Comments: yields increase with moisture



2 = <1008.6 kg/ha; 15.6%
and fertility. However, too high a moisture can



3 = <1479.4 kg/ha; 51.5%
lead to killing of plants. Too high fertility can



4 = <1950.2 kg/ha; 25.8%
lead to extra vegetative growth that may not



5 = >1950.1 kg/ha; 1.4%
lead to higher yields. The optimum population



avg. = 1114.6 kg/ha,
depends on the phenotype (Branching Style,



std = 331.2
Character No. 1; Number of Capsules per Leaf




Axil Character No. 2; and Maturity Class,




Character No. 3) and row width.


(11) RESISTANCE TO
Subjective rating
In a year when there is a drought, this rating


DROUGHT
Values: 0 to 8 scale
can be used to differentiate the effects of the


The relative amount of
7 = Little effect from drought
different lines. This is a highly subjective


resistance to drought
4 = Medium effect from
rating requiring a rater that is familiar with the



drought
performance of the line under normal



1 = Considerable effect from
conditions. The rating is based on how the



drought
drought changes the line from normal. Thus, a



Intermediate values are used.
short line that does not change significantly in



S28 has not been exposed to
a drought may have a higher rating than a tall



a severe drought to date
line which is affected by the drought even



Distribution within Sesaco
though the taller line is taller in the drought



based on stable lines in the
than the short line.



crossing program in 2000



(Total number of samples



tested = 632)



low = 0; high = 8



1 = <1.6; 0.8%



2 = <3.2; 28.0%



3 = <4.8; 36.1%



4 = <6.4; 34.5%



5 = >6.3; 0.6%



avg. = 4.1, std = 1.2


(12) LEAF LENGTH
Unit of measure: cm
Select one leaf per node to measure from


The length of the leaf
Values: average of 3 plants
the 5th, 10th, and 15th nodes from the base of


blade from the base of the
S28 = 19.5 cm for 5th node
the plant. All the leaves for one line should be


petiole to the apex of the
in 2002 in Uvalde nursery;
collected at the same time. Some lines retain


leaf from the 5th, 10th, and
14.5 cm for 10th node in
the cotyledons, and the cotyledon node does


15th nodes
2002 in Uvalde nursery;
not count as a node. In some lines the lowest



and 12.1 cm for 15th node in
leaves abscise leaving a scar on the stem.



2002 in Uvalde nursery
Abscised nodes should be counted. In lines



Distribution within Sesaco
with alternate leaves, one node is counted for



for 5th leaf based on stable
each pair of leaves. In some lines in parts of the



lines in the crossing program
plant there are three leaves per node which



in 2002 (Total number of
should be counted as one node.



lines tested = 196 with 711
The leaves continue growing in the first few



samples)
days after they have separated from the



low = 13.8 cm; high = 42.5 cm
growing tip. The choosing of leaves should be



1 = <19.5 cm; 34.7%
done a minimum of 5 days after the 15th node



2 = <25.3 cm; 48.0%
has appeared. Timing is important, because the



3 = <31.0 cm; 14.3%
plants will begin to shed their lower leaves



4 = <36.8 cm; 1.5%
towards the end of their cycle.



5 = >36.7 cm; 1.5%
There are lines that have less than 15 nodes.



avg. = 21.5 cm, std = 4.4
In this case, the highest node should be taken



Distribution within Sesaco
and the node number annotated to the



for 10th leaf based on stable
measurements.



lines in the crossing program
There can be as much as 6 mm difference



in 2002 (Total number of
between a green leaf and a dry leaf. The



lines tested = 196 with 711
measurements can be done on a green or dry



samples)
leaf as long as any comparison data with other



low = 9.3 cm; high = 32.9 cm
lines is based on the same method.



1 = <14.0 cm; 22.4%
Generally, the lowest leaves increase in size



2 = <18.7 cm; 41.8%
until the 4th to 6th node and then they decrease



3 = <23.5 cm; 20.9%
in size. This applies to all measurements (Leaf



4 = <28.2 cm; 10.2%
Length (Character No. 12), Leaf Blade Length



5 = >28.1 cm; 4.6%
Character No. 13), Leaf Blade Width



avg. = 17.9 cm, std = 4.8
(Character No. 14), and Petiole Length



Distribution within Sesaco
(Character No. 15). Generally, the width will



for 15th leaf based on stable
decrease at a greater rate than the length.



lines in the crossing program
Comments: the length is dependent on the



in 2002 (Total number of
amount of moisture and fertility. Higher



lines tested = 196 with 711
moisture and fertility increase the length. Leaf



samples)
size also appears to be affected by light



low = 4.4 cm; high = 26.2 cm
intensity. In Korea, the Korean lines have



1 = <8.8 cm; 5.1%
much larger leaves than in Oklahoma. In



2 = <13.1 cm; 42.9%
Korea, there is more cloud cover and a general



3 = <17.5 cm; 29.8%
haze than in Oklahoma.



4 = <21.8 cm; 15.8%



5 = >21.7 cm; 6.6%



avg. = 14.3 cm, std = 4.2


(13) LEAF BLADE
Unit of measure: cm
See Leaf Length (Character No. 12) on how


LENGTH
Values: average of 3 plants
to collect leaves. The measurement does not


The length of the leaf
S28 = 19.5 cm for 5th node
include Petiole Length (Character No. 15). In


blade from the base of the
in 2002 in Uvalde nursery;
some leaves the blade on one side of the petiole


leaf blade to the apex of
14.5 cm for 10th node in
starts before the other side. This measure


the leaf from the 5th, 10th,
2002 in Uvalde nursery;
should start from the lowest blade side. There


and 15th nodes
and 12.1 cm for 15th node in
are leaves that have enations where a blade



2002 in Uvalde nursery
starts and then stops. The enations are not



Distribution within Sesaco
considered part of the leaf blade because they



for 5th leaf based on stable
are very irregular from plant to plant and



lines in the crossing program
within a plant.



in 2002 (Total number of
Comments: see Leaf Length (Character No.



lines tested = 196 with 711
12) for effects of environment



samples)



low = 9.0 cm; high = 25.5 cm



1 = <12.3 cm; 14.3%



2 = <15.6 cm; 60.2%



3 = <18.9 cm; 20.9%



4 = <22.2 cm; 3.1%



5 = >22.1 cm; 1.5%



avg. = 14.4 cm, std = 2.4



Distribution within Sesaco



for 10th leaf based on stable



lines in the crossing program



in 2002 (Total number of



lines tested = 196 with 711



samples)



low = 8.3 cm; high = 23.4 cm



1 = <11.3 cm; 18.9%



2 = <14.3 cm; 42.9%



3 = <17.4 cm; 25.0%



4 = <20.4 cm; 9.2%



5 = >20.3 cm; 4.1%



avg. = 13.9 cm, std = 3.0



Distribution within Sesaco



for 15th leaf based on stable



lines in the crossing program



in 2002 (Total number of



lines tested = 196 with 711



samples)



low = 4.2 cm; high = 20.7 cm



1 = <7.5 cm; 2.0%



2 = <10.8 cm; 36.7%



3 = <14.1 cm; 37.8%



4 = <17.4 cm; 16.3%



5 = >17.3 cm; 7.1%



avg. = 12.0 cm, std = 3.0


(14) LEAF BLADE
Unit of measure: cm
See Leaf Length (Character No. 12) on how


WIDTH
Values: average of 3 plants
to collect leaves. There are many leaves that


The width of the leaf
S28 = 19.5 cm for 5th node
are not symmetrical with lobbing on one side


blade measured across the
in 2002 in Uvalde nursery;
and not the other. The width should still be


leaf blade at the widest
14.5 cm for 10th node in
measured across the widest point on a line


point at the 5th, 10th and
2002 in Uvalde nursery;
perpendicular to the main vein of the leaf.


15th nodes
and 12.1 cm for 15th node in
On some lines the width exceeds the length,



2002 in Uvalde nursery
particularly on lobed leaves.



Distribution within Sesaco
Comments: see Leaf Length (Character No.



for 5th leaf based on stable
12) for effects of environment



lines in the crossing program
The widest leaves are lobed. Normally, the



in 2002 (Total number of
leaves have turned from lobed to lanceolate by



lines tested = 196 with 711
the 10th leaf with the exception of the tropical



samples)
lines.



low = 3.4 cm; high = 31.0 cm



1 = <8.9 cm; 53.1%



2 = <14.4 cm; 33.7%



3 = <20.0 cm; 9.7%



4 = <25.5 cm; 2.6%



5 = >25.4 cm; 1.0%



avg. = 9.6 cm, std = 4.3



Distribution within Sesaco



for 10th leaf based on stable



lines in the crossing program



in 2002 (Total number of



lines tested = 196 with 711



samples)



low = 1.3 cm; high = 17.6 cm



1 = <4.6 cm; 69.4%



2 = <7.8 cm; 25.0%



3 = <11.1 cm; 4.6%



4 = <14.3 cm; 0%



5 = >14.2 cm; 1.0%



avg. = 4.3 cm, std = 2.2



Distribution within Sesaco



for 15th leaf based on stable



lines in the crossing program



in 2002 (Total number of



lines tested = 196 with 711



samples)



low = 0.7 cm; high = 6.0 cm



1 = <1.8 cm; 29.1%



2 = <2.8 cm; 48.0%



3 = <3.9 cm; 15.3%



4 = <4.9 cm; 4.6%



5 = >4.8 cm; 3.1%



avg. = 2.3 cm, std = 0.9


(15) PETIOLE
Unit of measure: cm
See Leaf Blade Length (Character No. 13)


LENGTH
Values: average of 3 plants
on how to collect leaves. In some leaves, the


The length of the petiole
S28 = 19.5 cm for 5th node
blade on one side of the petiole starts before


from the base of the
in 2002 in Uvalde nursery;
the other side. This measure should end where


petiole to the start of the
14.5 cm for 10th node in
the earliest blade starts. There are leaves that


leaf blade at the 5th, 10th,
2002 in Uvalde nursery;
have enations where a blade starts and then


and 15th nodes
and 12.1 cm for 15th node in
stops. The enations are not considered part of



2002 in Uvalde nursery
the leaf blade because they are very irregular



Distribution within Sesaco
from plant to plant and within a plant and



for 5th leaf based on stable
should be measured as part of the petiole.



lines in the crossing program
Comments: see Leaf Length (Character No.



in 2002 (Total number of
12) for effects of environment



lines tested = 196 with 711



samples)



low = 3.0 cm; high = 17.0 cm



1 = <5.8 cm; 35.2%



2 = <8.6 cm; 39.8%



3 = <11.4 cm; 19.4%



4 = <14.2 cm; 4.1%



5 = >14.1 cm; 1.5%



avg. = 7.0 cm, std = 2.5



Distribution within Sesaco



for 10th leaf based on stable



lines in the crossing program



in 2002 (Total number of



lines tested = 196 with 711



samples)



low = 1.0 cm; high = 14.2 cm



1 = <3.6 cm; 53.6%



2 = <6.3 cm; 31.6%



3 = <8.9 cm; 11.7%



4 = <11.6 cm; 2.0%



5 = >11.5 cm; 1.0%



avg. = 4.0 cm, std = 2.1



Distribution within Sesaco



for 15th leaf based on stable



lines in the crossing program



in 2002 (Total number of



lines tested = 196 with 711



samples)



low = 0.2 cm; high = 7.4 cm



1 = <1.6 cm; 38.8%



2 = <3.1 cm; 41.8%



3 = <4.5 cm; 13.3%



4 = <6.0 cm; 3.1%



5 = >5.9 cm; 3.1%



avg. = 2.3 cm, std = 1.3


(16) NUMBER OF
Unit of measure: Actual
The rating can be taken from about 60 days


CARPELS PER
number
after planting to all the way to the end of the


CAPSULE
Values:
crop.


The predominant number
2 = bicarpellate
There are many plants with mixed number


of carpels per capsule in
3 = tricarpellate
of carpels as follows:


the middle half of the
4 = quadricarpellate
1. Some bicarpellate plants will have one or


capsule zone
S28 = 2 for all crops in all
more nodes near the center of the capsule zone



nurseries
that have tri- and/or quadricarpellate capsules



Distribution within Sesaco
and vice versa.



based on the introductions
2. Most tri- and quadri-carpellate plants will



received in 1982–2001
begin and end with bicarpellate nodes.



(Total number of samples
3. Some plants have only one carpel that



tested = 2702)
develops. These capsules are generally bent,



2 = 97.6%
but on examination the 2nd carpel can be seen.



3 = 0.0004%
4. On all types, flowers may coalesce and



4 = 2.3%
double or triple the number of carpels.



Sesaco has not developed



lines with more than 2



carpels.


(17) CAPSULE
Unit of measure: cm
After the plants are physiologically mature,


LENGTH FROM
Values: taken on the median
take 2 capsules from five plants from the


10cap TEST
capsule in a 10 capsule
middle of the capsule zone. On three capsule


The length of the capsule
sample
per leaf axil lines, one central capsule and one


from the bottom of the
S28 = 2.42 cm in 2002 in
axillary capsule should be taken from the same


seed chamber to the top of
Uvalde nursery
leaf axil. The measurement is taken on the


the seed chamber from the
Distribution within Sesaco
median capsule of single capsule lines and on


outside of the capsule.
based on 10cap test in all
the median central capsule on three capsule


The tip of the capsule is
nurseries in 1997–2002
lines. The measurement is taken on dry


not included in the
(Total number of lines tested = 1,613
capsules because the length can shorten as


measurement.
with 8,285 samples)
much as one mm on drydown.



low = 1.3 cm; high = 4.5 cm
The 10 capsules can be sampled from



1 = <1.94 cm; 2.7%
physiological maturity through complete



2 = <2.58 cm; 67.9%
drydown without an effect on this character.



3 = <3.22 cm; 27.2%
Generally, the capsules in the middle of the



4 = <3.86 cm; 1.9%
capsule zone are the longest on the plant.



5 = >3.85 cm; 0.3%
Comments: the length of the capsule is



avg. = 2.44 cm, std = 0.33
dependent on the amount of moisture, fertility,




and population. Higher moisture and fertility




increase the length. Higher population




decreases the length even with adequate




moisture/fertility.


(18) SEED WEIGHT
Unit of measure: grams
See Capsule Length from 10cap Test


PER CAPSULE
Values: average of 10
(Character No. 17) for collection of capsules.


FROM 10cap TEST
capsules
The capsules should be dried, the seed threshed


The weight of the seed in
S28 = 0.242 g in 2002 in
out, and the seed weighed.


a capsule from the center
Uvalde nursery
The 10 capsules can be sampled from


of the capsule zone
Distribution within Sesaco
physiological maturity through complete



based on 10cap test in all
drydown without an effect on this character.



nurseries in 1997–2002
After drydown, only capsules with all their



(Total number of lines tested = 1,613
seed are taken. Thus, this test cannot be done



with 8,285 samples)
on shattering lines after drydown.



low = 0.053 g; high = 0.476 g
Generally, the capsules in the middle of the



1 = <0.138 g; 1.3%
capsule zone have the highest seed weight per



2 = <0.222 g; 47.6%
capsule on the plant.



3 = <0.307 g; 50.6%
Comments: see Capsule Length from 10cap



4 = <0.391 g; 1.1%
Test (Character No. 17) for the effects of



5 = >0.390 g; 0.1%
environmental factors.



avg. = 0.221 g, std = 0.039


(19) CAPSULE
Unit of measure: grams
See Capsule Length from 10cap Test


WEIGHT PER
Values: average of 10
(Character No. 17) for collection of capsules.


CAPSULE FROM
capsules
The capsules should be dried, the seed threshed


10cap TEST
S28 = 0.164 g in 2002 in
out, and the capsules weighed.


The weight of the capsule
Uvalde nursery
The 10 capsules can be sampled from


from the center of the
Distribution within Sesaco
physiological maturity through complete


capsule zone after the
based on 10cap test in all
drydown without an effect on this character.


seed has been removed
nurseries in 1997–2002
Generally, the capsules in the middle of the



(Total number of lines tested = 1,613
capsule zone have the highest capsule weight



with 8,285 samples)
per capsule on the plant.



low = 0.059 g; high = 0.395 g
Comments: see Capsule Length from 10cap



1 = <0.126 g; 22.6%
Test (Character No. 17) for the effects of



2 = <0.193 g; 69.1%
environmental factors.



3 = <0.261 g; 8.2%



4 = <0.328 g; 0.9%



5 = >0.327 g; 0.6%



avg. = 0.152 g, std = 0.036


(20) CAPSULE
Unit of measure: grams
The weight is derived by dividing the


WEIGHT PER CM
Values: average of 10
Capsule Weight per Capsule from 10cap Test


OF CAPSULE
capsules
(Character No. 19) by the Capsule Length from


The weight of a capsule
S28 = 0.068 g in 2002 in
10cap Test (Character No. 17).


per cm of capsule from
Uvalde nursery
The 10 capsules can be sampled from


the center of the capsule
Distribution within Sesaco
physiological maturity through complete


zone
based on 10cap test in all
drydown without an effect on this character.



nurseries in 1997–2002
Comments: this character is used instead of



(Total number of lines tested = 1,613
capsule width. Capsule width is difficult to



with 8,285 samples)
measure because there are so many variables in



low = 0.027 g; high = 0.123 g
a capsule. In a bicarpellate capsule, the width



1 = <0.046 g; 8.2%
differs when measuring across one carpel or



2 = <0.065 g; 55.5%
both carpels. Capsules can also vary through



3 = <0.085 g; 36.5%
the length of the capsule by being substantially



4 = <0.104 g; 4.4%
narrower at the bottom, middle or top of the



5 = >0.103 g; 0.5%
capsule. In 1997, four widths were measured



avg. = 0.063 g, std = 0.012
on each capsule and then averaged. This




average had a very high correlation to the




capsule weight per cm of capsule.




See Capsule Length from 10cap Test




(Character No. 17) for effects of environmental




factors


(21) VISUAL SEED
Subjective rating
When the plants in a plot are dry below


RETENTION
Values:
where the cutter bar would hit the plant, assign


Amount of seed in most of
Seed Retention
a rating based on the following guidelines. If


the capsules in the middle
8 = 100%
just identifying lines for further testing, use


half of the capsule zone
6 = 75%
V/X/W ratings. If identifying lines to use in a


when the plant(s) are dry
4 = 50%
crossing program to improve seed retention,


enough for direct harvest
2 = 25%
use 0–8 ratings. Ratings 6–8 can be seen


with a combine
0 = 0%
without removing the capsule from the plant.



Intermediate values can be
For the other ratings, the capsules must be



used.
opened. Rating is an overall subjective number



V = >74% seed retention
since retention can vary from plant to plant and



(sufficient seed retention for
even within a plant.



10cap testing)
The effects of the environment are not fully



X = <75% seed retention
known. There are indications that in drought



(unsuitable for direct harvest)
or very low fertility conditions, there is less



W = >74% seed retention
seed retention. When high propulations lead to



on weathering in field after
low moisture or fertility, there is less seed



rains and/or winds for more
retention. From normal conditions through



than 3 weeks following
high moisture/fertility conditions, there does



complete drydown
not appear to be an appreciable difference.



S28 = W for all crops in all



nurseries


(22) SHAKER
Unit of measure: Actual
See Capsule Length from 10cap Test


SHATTER
Number expressed as
(Character No. 17) for collection of capsules.


RESISTANCE
percentage
The capsules should be dried. The capsules


FROM 10cap TEST
Values: average of 10
should then be placed in flasks on a reciprocal


The amount of seed retention
capsules
shaker with a 3.8 cm stroke with 250


after the capsules are
S28 = 77.3% in 2002 in
strokes/min for 10 minutes (see U.S. Pat.


dry, inverted, and put
Uvalde nursery
No. 6,100,452). The seed that comes out of the


through a shaker
Distribution within Sesaco
capsules should be weighed as ‘out seed.’ The



based on 10cap test in all
retained seed should be threshed out of the



nurseries in 1997–2002
capsules and weighed to compute the ‘total



(Total number of lines tested = 1,613
seed’. The shaker shatter resistance is



with 8,285 samples)
computed as a percentage as follows: (total



low = 0; high = 100
seed - out seed)/total seed.



1 = <20; 12.9%
The 10 capsules can be sampled from



2 = <40; 6.9%
physiological maturity through complete



3 = <60; 23.4%
drydown without an effect on this character for



4 = <80; 47.7%
shatter resistant types. When taking capsules



5 = >79.9; 9.2%
after drydown, only capsules with all their seed



avg. = 55.9%, std = 23.9
are taken. Thus, this test cannot be done on




shattering lines after drydown.




Comments: there are indications that in




drought or very low fertility condition, there is




less seed retention. When high populations lead




to low moisture or fertility, there is less seed




retention. From normal conditions through




high moisture/fertility conditions, there does




not appear to be an appreciable difference in




seed retention. Lines with shaker shatter




resistance >64.9% are known as non-dehiscent




lines (see U.S. Pat. No. 6,100,452).


(23) CAPSULE
Subjective rating
The rating is based on visual seed retention


SHATTERING
Values:
and other visual observations. The plants


TYPE
SUS = Super-shattering (<2
remain standing in the field without shocking.


Amount of seed retention
visual seed retention -
GS plants can be identified while the plant


in a line or plant
equates to <25%)
is putting on capsules or at drydown because



SHA = Shattering (<4 visual
the carpels in the capsules do not form false



seed retention - equates to
membranes. There are plants that will have



<50%)
capsules with false membranes on the lower



SSH = Semi-shattering (4–6
and upper nodes but most of the capsules show



visual seed retention -
no false membranes.



equates to 50 to 75%)
ID plants can be identified during the



SR = Shatter resistant (>6
growing season in that they have enations on



visual seed retention without
the bottoms of the leaves. At dry down they are



id or gs alleles - equates to
more difficult to distinguish from other lines



>75%)
that have closed capsules (other than GS).



ID = Indehiscent (presence
There is less of a suture than other capsule



of id/id with capsule closed)
types.



IDO = Indehiscent (presence
SUS, SHA, SSH, and SR are defined by



of id/id with capsule open at
Visual Seed Retention (Character No. 21).



tip)
Comments: most environmental factors do



GS = Seamless (presence of
not have much of an effect on capsule



gs/gs with capsule closed)
shattering type other than to make it more



GSO = Seamless (presence of
difficult to distinguish in the overlap zone.



gs/gs with capsule open at
Generally, higher moisture, higher fertility, and



tip)
lower populations will increase the shattering a



S28 = SR in all crops in all
small amount - less than 10%.



nurseries
The wind can have a large effect in




decreasing the amount of seed retention. Rain,




dew and fog can also reduce seed retention.


(24) NON-DEHISCENT
Rating
Lines are designated as ND only after they


TEST
Values:
have undergone a minimum of 3 shaker shatter


A line that has passed the
ND = Non-dehiscent line
resistance tests. In order to be considered an


non-dehiscent test of
XX = Line that does not
ND variety, the line must pass the ND


having shaker shatter
pass the non-dehiscent test
threshold in multiple nurseries for multiple


resistance >64.9%. ND
S28 = ND for all crops in all
years. For example, S28 has a mean of 75.6%


lines should not have id or
nurseries
seed retention in 20 shaker shatter resistance


gs alleles.
Distribution within Sesaco
tests for 1997–2001.



based on 10cap test in all



nurseries in 1997–2001



(Total number of samples



tested = 3031)



ND = 56.8%



XX = 43.2%


(25) DAYS TO
Unit of measure: days
This data is taken as a date and later


FLOWERING
Values: number of days
converted to number of days. Flowering is


Number of days from
S28 = 41 days in 2002 in
defined as flowers that are open - not buds.


planting until 50% of the
Uvalde nursery
This is a somewhat subjective unit of


plants are flowering
Distribution within Sesaco
measure because there is little difference in the



based on lines in Uvalde
appearance of a line that has 40% and a line



nursery in 2000–2001
that has 60% of plants with flowers. In



(Total number of samples
addition, the plots are not walked every day,



tested = 1831)
and thus there is an estimate that the plot was



low = 33 days; high = 89 days
50% a few days before or after the date of data



1 = <44.2 days; 87.9%
collection.



2 = <55.4 days; 7.8%
Comments: flowering can be accelerated



3 = <66.6 days; 2.4%
by drought and it can be delayed by higher



4 = <77.8 days; 1.7%
moisture and/or fertility. Higher heat units will



5 = >77.7 days; 0.2%
decrease the days to flowering.



avg. = 40.9 days, std = 6.3
Some lines are photosensitive and will only




begin flowering at a certain number of hours of




daylight.




Start of flowering does not always equate to




start of capsule formation. Many lines will




flower and not set capsules from the first




flowers.


(26) DAYS TO
Unit of measure: days
This data is taken as a date and later


FLOWER
Values: number of days
converted to number of days. Flowering is


TERMINATION
S28 = 79 days in 2002 in
defined as flowers that are open - not buds. At


Number of days from
Uvalde nursery
the end of the flowering period, the rate that a


planting until 90% of the
Distribution within Sesaco
plant puts on open flowers is reduced. Thus,


plants have stopped
based on lines in Uvalde
there can be more than 10% of plants with buds


flowering
nursery in 2000–2001
and still have reached this measure since there



(Total number of samples
will not be more than 10% flowering any one



tested = 2668)
day.



low = 61 days; high = 114 days
This is a somewhat subjective unit of



1 = <71.6 days; 21.1%
measure because there is little difference in the



2 = <82.2 days; 61.5%
appearance of a line that has 85% and a line



3 = <92.8 days; 15.9%
that has 95% of plants with no flowers. In



4 = <103.4 days; 0.8%
addition, the plots are not walked every day,



5 = >103.3 days; 0.8%
and thus there is an estimate that the plot was



avg. = 77.1 days, std = 6.9
90% a few days before or after the date of data




collection. Another problem is that under low




moisture conditions the plots can reach the




90% mark only to begin flowering again after a




rain. In those cases the data is adjusted to the




later number of days.




The measure is based on the number of




plants and not the number of flowering heads.




The branches will stop flowering before the




main stem, and thus the plot will appear like




there are more plants not flowering.




Comments: flower termination can be




accelerated by lower moisture and/or fertility,




and it can be delayed by higher moisture, and/or




fertility. Higher heat units will decrease the




Days to Flower Termination. It is known that




there are lines that stop flowering sooner than




expected in northern latitudes, but it is not




known if this is due to shorter photoperiod or




cool temperatures.


(27) DAYS TO
Unit of measure: days
This data is taken as a date and later


PHYSIOLOGICAL
Values: number of days
converted to number of days. Physiological


MATURITY
S28 = 96 days in 2002 in
maturity (PM) is defined as the point at which


Number of days from
Uvalde nursery
3⁄4 of the capsules have seed with final color.


planting until 50% of the
Distribution within Sesaco
In most lines, the seed will also have a seed


plants reach physiological
based on lines in Uvalde
line and tip that are dark.


maturity
nursery in 2000–2001
This is a somewhat subjective unit of



(Total number of samples
measure because there is little difference in the



tested = 2374)
appearance of a line that has 40% and a line



low = 77 days; high = 140 days
that has 60% of plants with PM. In addition,



1 = <89.6 days; 16.8%
the plots are not walked every day, and thus



2 = 102.2 days; 58.0%
there is an estimate that the plot was 50% a few



3 = <114.8 days; 23.6%
days before or after the date of data collection.



4 = <127.4 days; 1.4%
Comments: The concept of physiological



5 = >127.3 days; 0.2%
maturity in sesame was developed by M. L. Kinman



avg. = 97.1 days, std = 7.1
(personal communication) based on




the concept of determining the optimum time




to cut a plant and still harvest 95–99% of the




potential yield. When the seed has final color,




the seed can germinate under the proper




conditions. If the plant is cut at physiological




maturity, most of the seed above the 3⁄4 mark




will continue maturing enough for germination,




but will not have as much seed weight. Since in




even a fully mature plant, there is less seed




weight made at the top of the plant, this loss of




seed weight does not seriously affect the




potential seed weight of the plant.




Although present harvest methods let the




plants mature and go to complete drydown, PM




is important because after that point, the crop is




less susceptible to yield loss due to frost or




disease. The PM is also important if the crop




is to be swathed or dessicants are to be applied.




Physiological maturity can be accelerated




by lower moisture and/or fertility, and it can be




delayed by higher moisture and/or fertility.




Higher heat units will decrease the days to




physiological maturity. Cool weather can delay




physiological maturity.


(28) SEED COLOR
Subjective rating
This data is taken in the laboratory with the


The color of the seed coat
Values:
same lighting for all samples. The seed from



WH = White
the whole plant is used.



BF = Buff
Seed coat color is taken on mature seeds. If



TN = Tan
there is any abnormal termination, the colors



LBR = Light brown
are not quite as even. The color of immature



GO = Gold
seed varies. Usually light seeded lines have tan



LGR = Light gray
to light brown immature seed; tan, light brown,



GR = Gray
gold, brown light gray, and gray lines have



BR = Brown
lighter immature seed; black lines can have tan,



RBR = Reddish brown
brown, or gray immature seed.



BL = Black
Usually, moisture, fertility, population and



S28 = BF in all crops in all
light intensity do not have an effect on seed



nurseries
coat color. Light colored seeds in a drought



Distribution within Sesaco
may have a yellowish tinge. Seeds in some



based on seed harvested in all
lines in the tan, light brown and gold range can



nurseries in 1982–2001 (Total
change from year to year among themselves.



number of samples tested = 161,809)



WH = 0.8%



BF = 74.8%



TN = 9.0%



LBR = 1.4%



GO = 1.5%



LGR = 0.6%



GR = 1.4%



BR = 6.5%



RBR = 0.6%



BL = 3.5%


(29) SEED WEIGHT -
Unit of measure: grams
See Capsule Length from 10cap Test


100 SEEDS FROM
Values: average of 3 samples
(Character No. 17) for collection of capsules.


10cap TESTc
S28 = 0.334 g in 2002 in
Count out 100 seeds and weigh. The seed


Weight of 100 seeds taken
Uvalde nursery
must be dry.


from the 10cap tests
Distribution within Sesaco
Comments: the weight increases with


which are taken from the
based on stable lines in all
higher moisture/fertility. Generally, the weight


middle of the plant.
nurseries in 1997–2002
is lighter than the seed weight taken from the



(Total number of lines tested = 820
10cap test.



with 2,899 samples)



low = 0.200 g; high = 0.455 g



1 = <0..251 g; 10.1%



2 = <0.302 g; 48.4%



3 = <0.353 g; 34.0%



4 = <0.404 g; 7.2%



5 = >0.403 g; 0.2%



avg. = 0.298 g, std = 0.04


(30) UVALDE KILL
Subjective rating
On the week a plot reaches PM, a rating is


RESISTANCE
Values: ratings are based on
assigned. The ratings are then taken for 2


The amount of plants
the number of plants killed in
additional weeks. The three ratings are


killed by root rots in the
a plot. Before physiological
averaged for a final kill rating. For example, if


Sesaco nurseries in
maturity (PM), the following
a plot has a final kill of 766, the average for the


Uvalde, TX
ratings are used:
plot will be 6.33. When a value of 1 or 2 is



1 = >90% kill before Days to
assigned, there are no additional ratings and



Flowering Termination
there is no averaging.



(Character No. 26)
There are three root diseases that affect



2 = >90% kill between Days
sesame in Texas: Fusarium oxysporum,



to Flowering Termination
Macrophomina phaseoli, and Phytophtora



(Character No. 26) and Days
parasitica. Between 1988 and the present,



to Physiological Maturity
spores of these three have been accumulated in



(Character No. 27)
one small area of Uvalde, and thus it is an



After PM, the following
excellent screening area for the diseases.



ratings are used:
Although each root rot attacks sesame in a



3 = >90% kill
different way with different symptoms, no



4 = 50 to 89% kill
effort is made to differentiate which disease is



5 = 25 to 49% kill
the culprit in each plot. Pathological screenings



6 = 10 to 24% kill
in the past have found all 3 pathogens present



7 = less than 10% kill
in dead plants.



8 = no kill
Comments: normally, the ratings will



S28 = 7.71 in 2002 in
decrease a maximum of one value per week.



Uvalde nursery
There is an overlap between any two ratings,



Distribution within Sesaco
but this is overcome to a certain extent by



based on lines in Uvalde
using three ratings over 2 weeks.



nursery in 2000–2001
The amount of kill is usually increased with



(Total number of samples
any type of stress to the plants. Drought can



tested = 3045)
increase the amount of Macrophomina; too



low = 1.00; high = 8.00
much water can increase the amount of



1 = <1.6; 1.7%
Phytophtora; high temperatures and humidity



2 = <3.2; 16.7%
can increase the amount of Fusarium and



3 = <4.8; 38.7%
Phytophtora. High population can increase all



4 = <6.4; 31.2%
three diseases.



5 = >6.3; 11.6%
The ratings for any one year can be used to



avg. = 4.52, std = 1.49
compare lines grown in that year, but should




not be used to compare lines grown in differnt




years. The amount of disease in any one year




is highly dependent on moisture, humidity, and




temperatures.


(31) RESISTANCE TO
Subjective rating
Ratings can be done in several ways:


FUSARIUM WILT
Values: 0 to 8 scale
1. Take ratings after the disease is no longer


(F. oxysporum)
% of infected plants
increasing


Amount of resistance to
8 = Zero disease
2. Take ratings on consecutive weeks until


Fusarium wilt
7 = <10% infected
disease is no longer increasing and average



4 = 50% infected
ratings.



1 = >90% infected
3. Take periodic ratings and average ratings.



0 = all infected
Comments: Fusarium has been a problem



NT = not tested
in South Texas, particularly on fields that have



NEC = no economic
been planted with sesame before. Normally,



damage - not enough disease
only the Uvalde Kill Resistance (Character No.



to do ratings
30) rating is taken.



S28 = 7 in 2002 in Uvalde



nursery


(32) RESISTANCE TO
Subjective rating
See Methodology for Resistance to Fusarium


PHYTOPHTORA
See Values for Fusarium
Wilt (Character No. 31)


STEM ROT (P. parasitica)
S28 = NT
Comments: Phytophtora has been a


Amount of resistance to

problem in Arizona and Texas, particularly on


Phytophtora stem rot

fields that have been over-irrigated. Normally,




only the Uvalde Kill Resistance (Character No.




30) rating is taken.


(33) RESISTANCE TO
Subjective rating
See Methodology for Resistance to Fusarium


CHARCOAL ROT
See Values for Fusarium
Wilt (Character No. 31)


(Macrophomina
S28 = NT
Comments: Macrophomina has been a


phaseoli)

problem in Arizona and Texas, particularly on


Amount of resistance to

fields that go into a drought. Normally, only


Charcoal rot

the Uvalde Kill Resistance (Character No. 30)




rating is taken.


(34) RESISTANCE TO
Subjective rating
See Methodology for Resistance to Fusarium


BACTERIAL
See Values for Fusarium
Wilt (Character No. 31)


BLACK ROT
S28 = NT
Comments: this disease occurs occasionally


(Pseudomonas

when there is continual rainy weather with few


sesami)

clouds. In most years, the disease abates once


Amount of resistance to

the weather changes. No economic damage has


bacterial black rot

been noticed.


(35) RESISTANCE TO
Subjective rating
Ratings can be done in several ways:


SILVERLEAF
Values: 0 to 8 scale
1. Take ratings after the insects are no longer


WHITE FLY
8 = Zero insects
increasing.


(Bemisia
7 = Few insects
2. Take ratings on consecutive weeks until


argentifolii)
4 = Many insects
insects are no longer increasing and average


Amount of resistance to
1 = Insects killing the plants
ratings.


the silverleaf white fly
NT = not tested
3. Take periodic ratings and average ratings.



NEC = no economic
Comments: there have been very few years



damage - not enough insects
(1991–1995) where the incidence of silverleaf



to do ratings
white fly has affected nurseries or commercial



S28 = NT
crops. In most years, a few white flies can be




seen in the sesame with no economic damage.




In the middle 1990s, the USDA began




introducing natural predators of the silverleaf




white fly in the Uvalde area. It is not known if




the predators reduced the effects of the white




fly or there is a natural tolerance to white fly in




the current varieties. In the 2000 nursery,




some lines were killed by the white fly.




Higher temperatures decrease the number of




days between generations. There are




indications that higher moisture and fertility




increase the incidence of white flies, but there




is no definitive data.




The sweet potato white fly (Bemisia tabaci)




has been observed in nurseries since 1978




without any economic damage.


(36) RESISTANCE TO
Subjective rating
See Methodology for Resistance to Silverleaf


GREEN PEACH
See Values for White Fly
White Fly (Character No. 35)


APHIDS (Myzus
S28 = NT
Comments: there have been very few years


persicae)

(1990–1995) where the incidence of green


Amount of resistance to

peach aphid has affected nurseries or


the green peach aphid

commercial crops. In most years, a few aphids




can be seen in the sesame with no economic




damage. There have been many years in West




Texas when the cotton aphid has decimated the




cotton and did not build up on adjacent sesame




fields.




Higher moisture and fertility increase the




susceptibility to aphids.


(37) RESISTANCE TO
Subjective rating
See Methodology for Resistance to Silverleaf


POD BORERS
See Values for White Fly
White Fly (Character No. 35)


(Heliothis spp.)
S28 = NT
Comments: there have been very few years


Amount of resistance to

(1985) where the incidence of Heliothis has


pod borers

affected nurseries or commercial crops. In most




years, a few borers can be seen in the sesame




with no economic damage.


(38) RESISTANCE TO
Subjective rating
See Methodology for Resistance to Silverleaf


ARMY WORMS
See Values for White Fly
White Fly (Character No. 35)


(Spodoptera spp.)
S28 = NT
Comments: there have been very few years


Amount of resistance to

(1984–1987) where the incidence of


army worms

Spodoptera has affected commercial crops in




Arizona. In Texas, army worms have




decimated cotton and alfalfa fields next to




sesame without any damage to the sesame. It is




not known if the Arizona army worm is




different from the Texas army worm.


(39) RESISTANCE TO
Subjective rating
See Methodology for Resistance to Silverleaf


CABBAGE
See Values for White Fly
White Fly (Character No. 35)


LOOPERS (Pieris
S28 = NT
Comments: there have been very few years


rapae)

(1992–1993) where the incidence of cabbage


Amount of resistance to

loopers has affected nurseries. In commercial


cabbage loopers

sesame, cabbage loopers have been observed




with no economic damage.





aUvalde nursery as described above.


bSan Angelo nursery as described above.


cIn previous reports, the 100 seed weight was taken from the whole plant. The data for the whole plant can be inconsistent since it may reflect either single plant selections, best bulk plants, or all the plants in a segment of the plot. The seed weight from an entire segment of a plot or field is generally lower than the single or bulk plant selections by 0.01 to 0.02 grams because plants below the canopyare included in the sample. In 1998, there was an experiment on 53 lines, which took the 100 seed weight from 5 different segments of three plants and from the whole plant. The average difference between the middle section and the whole plant was 0.006 g. From 2002 onward, the data has been taken from the 10cap test instead of the whole plant. The sample sizes from the 10cap testing are much larger than the sample sizes from the whole plant.






In developing sesame varieties for the United States, there are five major characters that are critical: Shaker Shatter Resistance (Character No. 22), Uvalde Kill Resistance (Character No. 30), Days to Physiological Maturity (Character No. 27), Seed Color (Character No. 28), and Seed Weight—100 Seeds from 10 cap test (Character No. 29). The first three characters contribute to yield which is the ultimate determinant for the farmer to grow a variety. In improving the characters, the yields have to be comparable to or better than current varieties, or provide a beneficial improvement for a particular geographical or market niche. Shaker Shatter Resistance determines how well the plants will retain the seed while they are drying down in adverse weather. Uvalde Kill Resistance determines whether the plants can finish their cycle and have the optimum seed fill. Days to Physiological Maturity determines how far north and to which elevation the varieties can be grown. In the United States and Europe, the Seed Color is important since over 95% of the market requires white or buff seed. There are limited markets for gold and black seed in the Far East. All other colors can only be used in the oil market. Seed Weight—100 Seeds from 10 cap test determines the market for the seed. Lack of Uvalde Kill Resistance can reduce Seed Weight—100 Seeds from 10cap test. In parts of the United States where there is little rain in dry years Resistance to Drought (Character No. 11) becomes important in reducing yield and seed weight.
There are other characters important in developing commercial sesame varieties explained in Langham, D. R. and T. Wiemers, 2002, “Progress in mechanizing sesame in the US through breeding”, In: J. Janick (ed.), Trends in new crops and new uses, ASHS Press, Alexandria, Va. Branching Style (Character No. 1), Height of Plant (Character No. 5) and Height of First Capsule (Character No. 6) are important in combining. Capsule Zone Length (Character No. 7), Number of Capsule Nodes (Character No. 8), Average Internode Length within Capsule Zone (Character No. 9), and Seed Weight per Capsule (Character No. 18) are important in creating potential Yield at Drydown (Character No. 10). Leaf dimensions (Characters No. 12, 13, 14, and 15) are important in determining optimum populations. Number of Capsules per Leaf Axil (Character No. 2), Number of Carpels per Capsule (Character No. 16), Capsule Length (Character No. 17), Catpsule Weight per Capsule (Character No. 19),. and Capsule Weight per cm of Capsule (Character No. 20) are important in breeding for Visual Seed Retention (Character No. 21) which leads to testing for Shaker Shatter Resistance (Character No. 22) and determining the C,apsule Shattering Type (Character No. 23). Days to Flowering (Character No. 25), Days to Flower Termination (Character No. 26), and Days to Physiological Maturity (Character No. 27) are highly correlated and important in determining the geographical range for the variety. In the United States, resistance to diseases and pests (Characters No. 31 to 39) are critical to allow the crop to reach maturity and harvest.
FIG. 2 provides the Shaker Shatter Resistance (Character No. 22) of all the varieties released by Sesaco since 1981. The figures are the mean from all testing in all nurseries from 1997 to 2002. S01 was an indehiscent variety with excellent seed retention, but the seed could not be threshed out without making the seed unmarketable. S02 through S10, S12, and S14 were developed for swathing at physiological maturity, drying in windrows, and then picking up with a combine. All of these varieties had good yields when there was little rain and high temperatures at harvest time. In the bad weather, the yields were reduced. Attempts were made to let these varieties dry down standing and then combining, but the yields were not commercially adequate. S11 was the first variety that could be left standing for harvest with adequate yields in normal weather. With the exception of S17, varieties S15 through S22 were released for specific niches. S17 replaced S11 in most locations until it was replaced by S23 and S24. In 2001, S25 replaced S23. S23 is considered to be the minimum acceptable Shaker Shatter Resistance for commercial use. S24, S25, S26, and S28 all have Shaker Shatter Resistance in the mid seventy percent level, which is considered the optimum level. There are some disadvantages associated with higher Shaker Shatter Resistance ratings: (1) capsules that have smaller openings at the top of the capsule can have a higher Shaker Shatter Resistance rating but the smaller opening can also result in mold in hot humid conditions, and (2) capsules that have a higher Shaker Shatter Resistance rating may require more force in the combine to release the seed from the capsules resulting in seed damage.
FIG. 3 provides the Uvalde Kill Resistance (Character No. 30) of all the varieties released by Sesaco since 1981 as compared in the Uvalde nursery in 2002 using all plots. Uvalde Kill Resistance is a composite rating of resistance to three root rots: Fusarium, Phytophtora, and Macrophomina. When sesame is first introduced into a growing area, there are few disease problems, but over time the spores of these fungi accumulate and disease resistance becomes critical. When sesame was first introduced in Uvalde in 1988, the yields were high. As farmers planted on the same fields in subsequent years, the yields decreased. S11 was very susceptible to these root rots, and thus, it was replaced by S17, which was subsequently replaced by S23 and S24. In 2001, S25 replaced S23. S26 was an improvement over S24 and S25, and S28 has even better kill resistance with 7.71.
FIG. 4 provides the mean Days to Physiological Maturity (Character No. 27) of all the varieties in 2002 in the Uvalde nursery. In the United States, sesame is currently grown from South Texas to southern Kansas. The growing window of a crop is determined by the earliest the crop can be planted in the spring as the ground warms up, and the onset of cold weather in the fall. Current sesame varieties require about 21° C. ground temperature to establish an adequate population. In most years, the ground is warm enough in South Texas in middle April and in southern Kansas in late May. Current sesame varieties require night temperatures above 5° C. for normal termination. In most years, the night temperatures are warm enough in South Texas until middle November and in southern Kansas until middle October. There have been years when cold fronts affect the growth of sesame in the middle of September in the north. East of Lubbock, Texas, the elevations begin climbing towards the Rocky Mountains, and there are later warm temperatures in the spring and earlier cold temperatures in the fall. In all years, if the sesame is planted as early as temperatures allow, lines with Days to Physiological Maturity of 105 days or less will have no problems. However, most areas are rainfed, and it is essential to have a planting rain before planting the sesame. Thus, the earlier the Days to Physiological Maturity of the variety, the more flexibility the farmers have with planting date. In South Texas, the goal is to have varieties with a Days to Physiological Maturity of less than 110 days while in southern Kansas the goal is less than 90 days. The mean Days to Physiological Maturity for S26 in 2002 was 102 days, and thus, S26 is limited in terms of planting date and geographical distribution. For S28, Days to Physiological Maturity of 96 days is a significant improvement that allows sesame to be planted further north and at higher elevations.
FIG. 5 provides the average Seed Weight—100 Seeds from the 10 cap test (Character No. 29) of all varieties between 1997 and 2002. The hulled market is the premium use of sesame in the United States and Europe. In recent years, hulled sesame processors have been increasing the specifications of Seed Weight—100 Seeds from the 10 cap test to between 0.31 and 0.33 g, and larger seed is preferable for hulled products used on top of breads and buns. To date, the Sesaco varieties with the highest Seed Weight—100 Seeds from the 10cap test have had marginal Shaker Shatter Resistance (Character No. 22) and poor Uvalde Kill Resistance (Character No. 30). Some markets have no specifications on seed weight, but larger seed is still desirable. The mean Seed Weight—100 Seeds from the 10cap test for S28 for all years is 0.338 which is comparable to S26 and larger than S24 and S25, and exceeds the specifications for hulled sesame.
Prior to 1988, all of the commercial sesame in the United States was grown in Ariz. S01 through S10, S12, and S14 were specifically developed for Arizona From 1988 to 1991, there was a transition from Arizona to Texas In 1996, sesame spread into Oklahoma, and in 1998, into Kansas. In introducing sesame to Texas, the swathing technology was tried on S07 and S10, but farmers did not have the proper equipment; the plants fell into the furrows and could not be picked up; and/or blowing sand covered parts of the windrows. S11 was the first variety that could be combined directly, and S11 persisted until 1995. However, in addition to being susceptible to root rots, S11 was also susceptible to pests such as green peach aphids (Myzus persicae) (Character No. 36) and silverleaf white fly (Bemisia argentifolii) (Character No. 35), and S11 had too long a Days to Physiological Maturity (Character No. 27) (109 days) for West Texas S15 was introduced for West Texas, but shorter Days to Physiological Maturity (107 days) was still not early enough and the Shaker Shatter Resistance (Character No. 22) (38.7% seed retention) was not adequate. S16 was released specifically because it was tolerant to the white fly, but it did not have good Shaker Shatter Resistance (42% seed retention) or Uvalde Kill Resistance (Character No. 30) (2.0 rating), and it was susceptible to lodging. S17 had better Uvalde Kill Resistance (5.56 rating), aphid resistance, and white fly resistance than S11. With an earlier Days to Physiological Maturity (98.5 days), S17 was the first variety that was suitable for West Texas and southern Kansas; however, S17 was susceptible to lodging. S18 and S21 were released for flavor for the Japanese organic market. S19 had improved lodging resistance over S17, but the yields in non-windy areas were not as good as S17. S20 and S22 were released because of excellent drought resistance. In the rainfed areas of Central Texas, the yields of S20 and S22 were higher than S17, the large seed was desirable to processors, and no root rots had been seen. However, the root rot spores began accumulating, and the S20 and S22 had to be discontinued. S24 replaced S17 based on higher Shaker Shatter Resistance (74% for S24 compared to 61% for S17), better Uvalde Kill Resistance (rating of 6.52 for S24 compared to 6.00 for S17), shorter Days to Physiological Maturity (95 days for S24 compared to 104 for S17), and larger Seed Weight—100 Seeds from 10cap test (Character No. 29) (0.304 g for S24 compared to 0.272 g for S17). S23 was used in southern Kansas and northern Oklahoma because of a shorter Days to Physiological Maturity than S24 (93 days for S23 compared to 95 days for S24), but the Seed Weight—100 Seeds from 10cap test was marginal (0.263 g for S23 compared to 0.304 g for S24). S25 replaced S23 in southern Kansas and northern Oklahoma because of comparable Days to Physiological Maturity (92 days for S25 compared to 93 days for S23) and Uvalde Kill Resistance (rating of 6.89 for S25 compared to 7.00 for S23). Compared to S23, S25 has better Shaker Shatter Resistance (72.5% seed retention for S25 compared to 65.7% seed retention for S23), but the major reason for the replacement is the Seed Weight—100 Seeds from 10cap test (0.303 g for S25 compared to 0.263 g for S23). S26 was developed for its Resistance to Drought (Character No. 11) with a rating of 6.4 as compared to the other two current varieties: 3.6 for S24 and 2.5 for S25. Compared to S24 and S25, S26 has comparable Shaker Shatter Resistance, better Uvalde Kill Resistance, and better Seed Weight—100 Seeds from 10cap test. In terms of the Days to Physiological Maturity S26 takes longer to mature than the current varieties (102 days for S26 compared to 92 days for S25 and 95 days for S24). As a result, there is a limit to the geographical range of S26 to the north.
S28 was selected specifically to provide a large seeded variety that could be planted further north than S26. However, in every environment, a line with comparable yield that matures earlier is an advantage because the harvest weather gets worse into the fall in all sesame growing areas. There is less daylight and less heat which slows down the drying of the crop. In terms of the other four of the five major characters that are critical: Shaker Shatter Resistance (Character No. 22), Uvalde Kill Resistance (Character No. 30), Seed Color (Character No. 28), and Seed Weight—100 Seeds from 10cap test (Character No. 29), S28 has buff seed color, slightly better shatter resistance, slightly better kill resistance, and comparable seed weight to S26.
Over the past 26 years, Sesaco has tested 2,918 introductions from all over the world. Commercial samples have been obtained from China, India, Sudan, Ethiopia, Burkina Faso, Nigeria, Mozambique, Pakistan, Myanmar, Bangladesh, Vietnam, Egypt, Mexico, Guatemala, Nicaragua, Venezuela, Thailand, Turkey, Upper Volta, Uganda, Mali, Kenya, Indonesia, Sri Lanka, Afghanistan, Philippines, Colombia, Ivory Coast, Gambia, Somalia, Eritrea, Paraguay, and El Salvador. Additional research seed has been received from the commercial countries and from many other countries such as Australia, Iraq, Iran, Japan, Russia, Jordan, Yemen, Syria, Morocco, Saudi Arabia, Angola, Argentina, Peru, Brazil, Cambodia, Laos, Sri Lanka, Ghana, Gabon, Greece, Italy, South Korea, Libya, Nepal, Zaire, and Tanzania. Research seed received from one country may have originated from another unspecified country. All the commercial and research introductions have Capsule Shattering Type (Character No. 23) of “SHA”.
Using selected characters from Table I, Table II provides a character differentiation between S28 and name cultivars from all over the world. From this comparison, S26 and S28 are distinguished as having advantages over all other cultivars tested by Sesaco.







TABLE II







Character Differentiation of Various Sesame Varietiesa









Character
Rating
Name cultivars tested by Sesaco





Capsule Shattering Type
SHA
From Venezuela: Venezuela 51, Venezuela 52,


(Character No. 23)

Guacara, Aceitera, Inamar, Acarigua, Morada,




Capsula Larga, Arawaca, Piritu, Glauca, Turen,




DV9, Fonucla, UCLA




From Mexico: Pachequeno, Yori, Anna, Teras,




Denisse, Canasta, Tehvantepeter




From India: TMV1, TMV3




From Turkey: Ozberk, Muganli, Gamdibi, Marmara




From Israel: DT45




From Guatemala: R198, R30




From Texas: Llano, Margo, Dulce, Blanco, Paloma,




Oro, Renner 1 and 2, Early Russian




From California: UCR3, UCR4, Eva, Calinda (Cal




Beauty)




From Thailand: KU18




From Korea: Danback, Gwansan, Pungyiong,




Suweon, Yuseong, Hanseon, Ahnsan, Kwangsan,




Jinback, Pungsan, Sodan, Yangheuk, Konheuk,




Whaheuck, Sungboon



SSH
From Sesaco: S02, S03, S04, S05, S06, S07, S08,




S09, S10, S12, S14



ID
From Venezuela: G2, Morada id




From Texas: Rio, Delco, Baco, Improved Baco,




Roy, Eli




From South Carolina: Palmetto




From California: UCR234




From Sesaco: S01



SR
All others, go to Non-dehiscent Test


Non-dehiscent Test
XX
From Sesaco: S11, S15, S16, S17, S18, S19, S20,


(Character No. 24)

S21



ND
All others, go to Branching Style


Branching Style
U
From Sesaco: S22, 19A, 11W


(Character No. 1)
B
All others, go to Number of Capsules per Leaf Axil


Number of Capsules per Leaf Axil
 3
From Sesaco: S23


(Character No. 2)
 1
From Sesaco: S24, S25, S26, S28


Seed Weight - 100 Seeds from
<0.31
From Sesaco: S24, S25


10cap test (g) (Character No. 29)
>0.31
From Sesaco: S26, S28





aSHA = shattering; SSH = semi-shattering; ID = indehiscent; SR = shatter resistant; XX = not non-dehiscent according to the teachings of U.S. Pat. No. 6,100,452; ND = non-dehiscent according to the teachings of U.S. Pat. No. 6,100,452; U = uniculm branching style; and B = true branching.






Sesame seed is still internationally traded like a spice wherein many of the trades are based on specific characteristics exhibited in samples of the seed being sold and on its intended use and market; these characteristics include but are not limited to admixture purity, color mixture, oil content, free fatty acid content and flavor. In the U.S., premium seed is traded based on purity, seed size, and color. With a recent surplus in the sesame supply in the world, optimal seed size and color have become very important for commercial success. Seed Weight—100 Seeds from 10 cap test (Character No. 29), an indicator of seed size, can be easily measured, but Seed Color (Character No. 28) is very subjective. Most Sesaco varieties are termed buff but they range from buff to light buff to white depending on harvest conditions. In 2002, sesame processors preferred the color of S26 seed so much better than the color of S24 and S25 that, in 2003, they were willing to buy S24 and S25 only at a discounted price. S28 has a seed color comparable to that of S26, one factor which supports its commercial success. There are other morphological differences between S24/S25 and S26/S28, but the differentiation shown in Table II is based on Seed Weight—100 Seeds from 10cap test (Character No. 29) because, commercially, it is the most significant difference.
Table III provides a comparison of S26 and S28, using all of the characters listed in Table I. In Table III, some of the values are different from the values used in FIG. 2–5. The values in Table III are from side by side plots grown under the same conditions, and the values in FIG. 2 to FIG. 5 are based on averages of all of the S26 and S28 plots grown in the nurseries and dates indicated. The major differences are indicated in the “Dif” column by a “C” for commercially important differences and an “M” for morphological differences.







TABLE III







Character Comparison of S26 and S28a












No.
Character
Year/nursery
S26
S28
Dif















1
Branching Style
All
B
B



2
Number of Capsules per Leaf Axil
All
1
1


3
Maturity Class
1998–2001 UV
M
M


4
Plant Phenotype
All
B1M
B1M


5
Height of Plant (cm)
2002 UV
165
149
C


6
Height of First Capsule (cm)
2002 UV
61
58


7
Capsule Zone Length (cm)
2002 UV
104
91
M


8
Number of Capsule Nodes
2002 UV
29
23
M


9
Average Internode Length within
2002 UV
3.6
4.0
M



Capsule Zone (cm)


10
Yield at Drydown (kg/ha)
2002 UV
1642
1624




2002 SA
1213
1292


11
Resistance to Drought
2000 SA
6.4
NT


12
Leaf Length (cm)
5th-2002 UV
25.3
19.5
M




10th-2002 UV
18.8
14.5
M




15th-2002 UV
14.9
12.1
M


13
Leaf Blade Length (cm)
5th-2002 UV
16.1
12.8
M




10th-2002 UV
14.5
12.0
M




15th-2002 UV
12.6
10.6
M


14
Leaf Blade Width (cm)
5th-2002 UV
15.5
10.8
M




10th-2002 UV
4.4
2.8
M




15th-2002 UV
2.0
1.6
M


15
Petiole Length (cm)
5th-2002 UV
9.2
6.7
M




10th-2002 UV
4.3
2.5
M




15th-2002 UV
2.3
1.5
M


16
Number of Carpels per Capsule
All
2
2


17
Capsule Length (cm)
2002 UV
2.26
2.42
M


18
Seed Weight per Capsule (g)
2002 UV
0.228
0.242
M


19
Capsule Weight per Capsule (g)
2002 UV
0.149
0.164
M


20
Capsule Weight per cm of
2002 UV
0.066
0.068



Capsule (g)


21
Visual Shatter Resistance
All
W
W


22
Shaker Shatter Resistance (%)
2002 UV
74.1
77.3
C


23
Capsule Shattering Type
All
SR
SR


24
Non-dehiscent Test
All
ND
ND


25
Days to Flowering
2002 UV
42
41


26
Days to Flower Termination
2002 UV
81
79


27
Days to Physiological Maturity
2002 UV
102
96
C


28
Seed Color
All
BF
BF


29
Seed Weight - 100 Seeds from
2002 UV
0.334
0.338



10cap test (g)


30
Uvalde Kill Resistance
2002 UV
7.21
7.71
C


31
Resistance to Fusarium Wilt (F. oxysporum)
2002 UV
7
7


32
Resistance to Phytophtora Stem Rot

NT
NT



(P. parasitica)


33
Resistance to Charcoal Rot

NT
NT



(Macrophomina phaseoli)


34
Resistance to Bacterial Black Rot

NT
NT



(Pseudomonas sesami)


35
Resistance to Silverleaf White Fly
2000 UV
NEC
NT



(Bemisia argentifolii)


36
Resistance to Green Peach Aphid
2000 UV
NEC
NT



(Myzus persica)


37
Resistance to Pod Borer (Heliothis
2001 UV
NEC
NT



spp.)


38
Resistance to Army Worms

NT
NT



(Spodoptera spp.)


39
Resistance to Cabbage Loopers

NT
NT



(Pieris rapae)





aB = true branches; UV = Uvalde nursery; M = medium maturity class of 95–104 days; E = early maturity class of 85–94 days; B1M = phenotype of true branches, single capsules per leaf axil, and medium maturity class of 95–104 days; B1E = phenotype of true branches, single capsules per lead axil, and early maturity class of 85–94 days; SA = San Angelo nursery; W = weather visual seed retention >75%;SR = shatter resistant; ND = non-dehiscent; BF = buff color; NT = not tested; and NEC = no economic damage - not enough disease to do ratings.






The data given in Table III show how S28 is morphologically different from S26, but these differences are not commercially significant. The three major areas of morphological differences are in the size of the leaves, the capsule zone in terms of internode length and number of nodes, and the capsule characters.
S28 has shorter leaves, narrower leaves, and shorter petioles than S26 as shown in Leaf Length (Character No. 12), Leaf Blade Length (Character No. 13), Leaf Blade Width (Character No. 14), and Petiole Length (Character No. 15). With the present agronomic practices, the sizes of the leaves have no commercial significance.
S28 has a longer Average Internode Length within Capsule Zone (Character No. 9) and less Number of Capsule Nodes (Character No. 8) than S26. Often within the same Plant Phenotype (Character No. 4), a lower number of nodes should lead to lower yields. However, S28 overcomes the deficiency by having slightly larger capsule dimensions in terms of longer Capsule Length (Character No. 17) and comparable Capsule Weight per cm of Capsule (Character No. 20). As mentioned before, there is a slight positive correlation between capsule width and Capsule Weight per cm of Capsule. Wongyai (Wongyai W. and S. Juttpornpong, 1992. “Indirect selection for seed weight in sesame using capsule size as a criteria,” Sesame and Safflower Newsletter, No. 7, p. 4–7) showed a positive correlation between capsule width and Seed Weight—100 Seeds from Whole Plant, which is a commercially significant difference as discussed below. Despite these slight positive correlations, capsule width is not used as a commercially significant selection filter; e.g., there are commercially viable lines with narrow capsules. The increased capsule dimensions lead to higher Seed Weight per Capsule. The major commercial differences between S28 and S26 varieties are in the maturity and the plant height.
Maturity Class (Character No. 3), Plant Phenotype (Character No. 4), Days to Flowering (Character No. 25), Days to Flower Termination (Character No. 26), and Days to Physiological Maturity (Character No. 27) are all related. Maturity Class and Plant Phenotype are based on Days of Physiological Maturity, and there is a high positive correlation between Days to Physiological Maturity and Days to Flowering and Days to Flower Termination. Basically, the later the start and end of flowering, the longer it takes to reach physiological maturity. Although the differences in start and ending of flowering in S26 and S28 are not significantly different, the physiological maturity of S28 is 6 days earlier than S26. This difference allows S28 to be planted further north and at higher elevations than S26. Also, because S28 matures earlier than S26, it can be harvested earlier, thus reducing exposure to adverse weather conditions during the fall harvest.
The Height of Plant (Character No.5) differences are not significant in 90% of sesame growing areas. However, in Uvalde, there are growers that push the sesame with higher fertility and more frequent irrigations. The goal for modem platform headers has been to keep the sesame below 152 cm so that the header does not push the sesame forward before it pulls the sesame into the cutter bar. Under these growing conditions, S26 can get as high as 165 cm, while the S28 is 149 cm high. Thus, for some equipment currently used in mechanized harvesting, S28 has an advantage over S26 by providing a shorter plant height.
For sesame grown in Uvalde and San Angelo, Yield at Drydown is an extremely important character in that it would be difficult to release a variety that does not have higher or comparable yields to the current commercial variety. In the case of S26 and S28, Yield at Drydown is not statistically different.
One of the major advantages of S26 as a variety is that it has higher Resistance to Drought than previous varieties. Since S28 has been selected, there has not been a drought to compare drought resistance in S28 to S26. However, there are several major criteria that have been shown to help in droughts: Days to Flowering (Character No. 25) and leaf dimensions. Days to Flowering is important because when the plants switch from the vegetative to the reproductive stage, they slow down both shoot and root development. Slowing down the root translates to shallower rooting. Since Days to Flowering between S26 and S28 differ by only 1 day, there is no significant difference in changing growth rates. In less leaf mass, there are less leaf surfaces for water transpiration, and thus, it is anticipated that S28 with its smaller leaf size may have an advantage over S26.
On May 20, 2004, a deposit of at least 2500 seeds of sesame plant S28 was made by Sesaco Corporation under the provisions of the Budapest Treaty with the American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, Va. 20110-2209, and the deposit was given ATCC Accession No. PTA-6008. This deposit will be maintained in the ATCC depository for a period of 30 years or 5 years after the last request or for the enforceable life of the patent, whichever is longer. Should the seeds from the sesame line S28 deposited with the American Type Culture Collection become unviable, the deposit will be replaced by Sesaco Corporation upon request. Sesaco agreed upon making its deposit that the United States Commissioner of Patents will have access to the deposit during pendency of the application and that all restrictions upon the availability to the public will be removed upon granting of the patent. Sesaco has received confirmation of viability of the biological materials at the time of deposit.
The foregoing invention has been described in some detail by way of illustration and characters for purposes of clarity and understanding. However, it will be obvious that certain changes and modifications may be practiced within the scope of the invention as limited only by the scope of the appended claims.

## Claims

1. A seed of sesame variety designated S28, a sample of said seed having been deposited under ATCC Accession No. PTA-6008.

2. A sesame plant or part thereof produced by growing a seed of sesame variety S28, a sample of said seed having been deposited under ATCC Accession No. PTA-6008.

3. Pollen of the sesame plant of claim 2.

4. A sesame plant having all the physiological and morphological characteristics of sesame variety S28, a sample of seed of said variety having been deposited under ATCC Accession No. PTA-6008.

5. A sesame plant having all the physiological and morphological characteristics of a sesame plant produced by growing seed of sesame variety S28, a sample of said seed having been deposited under ATCC Accession No. PTA-6008.

6. A tissue culture of regenerable cells produced from seed of sesame variety S28, a sample of said seed having been deposited under ATCC Accession No. PTA-6008.

7. A tissue culture of regenerable cells produced from sesame plant S28 or a part thereof wherein the plant or part thereof is produced by growing a seed of sesame variety S28, a sample of said seed having been deposited under ATCC Accession No. PTA-6008.

8. A sesame plant regenerated from a tissue culture of regenerable cells produced from seed of sesame variety S28, a sample of said seed having been deposited under ATCC Accession No. PTA-6008, wherein said regenerated sesame plant has all the physiological and morphological characteristics of said sesame variety S28.

9. A sesame plant regenerated from a tissue culture of regenerable cells produced from a sesame plant produced by growing a seed of sesame variety S28, a sample of said seed having been deposited under ATCC Accession No. PTA-6008, wherein said regenerated sesame plant has all the physiological and morphological characteristics of said sesame variety S28.

10. A method of producing sesame seed, comprising crossing a first parent sesame plant with a second parent sesame plant and harvesting the resultant sesame seed, wherein said first or second parent sesame plant was produced by growing seed of sesame variety S28, a sample of said seed having been deposited under ATCC Accession No. PTA-6008.