## Effluent Quality Assessment 

# Chevron Port Tampa Terminal Hillsborough County
NPDES #FL0035921 Sampled August 25, 2008

February 2009

Biology Section Bureau of Laboratories Division of Environmental Assessment and Restoration
Quality Manual No. 870346G NELAC Certification No. E31780

### 1 Chevron Port Tampa Terminal, 550 Commerce Stree t, Tampa, Hillsborough County, Florida, 33616. NPDES #FL0035921. Effluent samples for this facility were collected on August 25, 2008.



### Introduction Chevron Port Tampa Terminal is a petroleum product s bulk storage facility. The effluent discharge is intermittent and rainfall dependent and consists of contact and non-contact stormwater and hydrostatic test water. Discharges range between 2,000 and 1 00, 000 gallons per day on an as needed basis. Effluent is discharged via emergency Outfall D-003 and Outfall D-004 to the Class II marine waters ofPicnic Island Creek and thence to Tampa Bay. The points of discharge for Outfall D-003 and Outfall D004 are located approximately at latitude 27o 51’ 24” N, longitude 82o 32’ 13” W, and latitude 27o 51’ 29” N, longitude 82o 32’ 17” W, respectively. The facility does not have a design flow. The average annual daily flow through Outfall D-004 from August 2007 through July 2008 was 0.012 million gallons per day.

Hydrostatic test water is potable city water that is used to fill a storage tank after completion of modifications or repairs to the tank. The terminal does not discharge any proces s wastewater. All process water is delivered to a petroleum recycler. Load-rack sp ills and precipitate from storage racks are routed to an underground storage tank for subsequent off-site disposal.

The facility has no Administrative Orders, Consent Orders or mixing zones. A review of Discharge Monitoring Report Data for the past year revealed no permit violations. (Facility information provided by Jason Cohen FDEP Southwest District.)



### Methods Samples were collected from the EFF-4 NPDES sa mpling point for Outfall D-004 by Jason Cohen and Peter Wenner (FDEP Southwest District) following DEP-SOP-001/01 FS 2400 Wastewater Sampling. All DEP SOPs are available on the web at: http://www.floridadep.org/labs/qa/sops.htm

. See Appendix A for the completed chain of custody form.

The 48-hour acute screening toxicity tests and alga l growth potential test were performed following internal DEP SOPs TA07_01, TA07_02, and TA08_01 through TA08_09. All internal DEP SOPs are available on the web at: http://www.floridadep.org/labs/cgi-bin/sop/biosop.asp

. Although the 48-hour acute screening toxicity test does not reflect the required permit conditions, passing test results provide confidence that the required permit conditions would likely be met.

Prior to test initiation, bioassay samples were ae rated for 30 minutes at approximately 100 bubbles per minute, to decrease the dissolved oxygen co ncentration from 11.2 mg/L to 8.2 mg/L.

An aliquot of the AGP sample was salinity adjusted to meet the minimum salinity requirements of the test species. Both a freshwater and a marine AGP test were performed.



### Toxicity Test Results Tests were performed August 26 - 28, 2008.

Ceriodaphnia dubia 48-hour acute screening bioassay – LC 50 > 100%, 0% mortality* in 100% sample at 48 hours

Cyprinella leedsi 48-hour acute screening bioassay – LC 50 > 100%, 5% mortality* in 100% sample at 48 hours

The bioassay samples were not acutely toxic to the test organisms.

### 2 See Appendix B for bioassay bench sheets. See Appendix C for results of the last 20 standard reference toxicant (SRT) tests and the bench sheets and statisti cal analyses of the SRT tests that correspond to these facility tests. See Appendix D for test organism receipts.

*NOTE: In a 48-hour acute screening test of 100% e ffluent, mortality of <20% provides confidence that the effluent meets the acute toxicity criterion of Florida’s Surface Water Quality Standards (Rule 62- 302.500 (1)(a)4, F.A.C.). Mortalit y between 20-50% indicates low to moderate levels of toxicity, and further action may be required. Mortality of >50% indi cates that the effluent fails to meet the minimum requirement to discharge to waters of the state (Rule 62-4.24 4(3)(a), F.A.C.).



### Algal Growth Potential The effluent Algal Growth Potential (AGP) was 19.4 mg dry wt/L of the unicellular green alga, Pseudokirchneriella subcapitata , formerly known as Selenastrum capricornutum . Raschke and Shultz (1987) found that AGP values above 5.0 mg dry we ight/L represent a “problem” threshold for fresh receiving waters, implying nutrient enrichment. The analytical chemistry suggests that the effluent is nitrogen-limited. The predicted AGPs based on inorganic nitrogen and total nitrogen concentrations were 1.5 (±20%) mg dry wt/L and 76.2 (±20%) mg dry w t/L, respectively (Table 1; Miller et al. 1978).

The salinity-adjusted effluent AGP was 3.0 U (U - Ma terial was analyzed for but not detected; the report value is the minimum detection limit) mg dry wt/L of the saltwater species Dunaliella tertiolecta. Raschke and Shultz found that AGP values above 10.0 mg dry we ight/L represent a “problem” threshold for marine receiving waters, implying nutrient enrichment (perso nal communication, Marshall Faircloth, et. al. FDEP, with Ron Raschke USEPA Region 4 1987). One potentia l reason for the lack of growth in the salinityadjusted sample is precipitation of enough orthophosphate by the added sa lts that the sample became extremely phosphorus-limited (Manahan 1994).

See Table 1 for AGP results.



### Chemistry Results Total residual chlorine and total ammonia were not dete cted in the bioassay sample in the laboratory. The total ammonia concentration in the sample coll ected and preserved for chemical analysis was 0.034 mg/L. Based on the pH, salinity, and temperature of t he effluent as collected, the calculated unionized ammonia concentration was < 0.02 mg/L.

Chemical substances of concern were not detected in t he effluent at levels that did not comply with Class II marine water quality criteria (62-302.530, F.A.C.) or permit conditions. It should be noted that due to a priority Central Laboratory project, samples for meta l and organic constituent analyses were not collected.

The effluent’s fecal coliform bacteria concentration (5 90 colony forming units per 100 ml (CFU)) exceeded the water quality standard for fecal colifor m bacteria in Clas s III marine waters ( ≤ 400 CFU/100 ml; 62- 302.530(6), F.A.C.)

See Table 2 for results of analytes detected in t he effluent, and corresponding limits. See Appendix E for a complete list of chemical analyses performe d. See Appendix F for bioassay lab equipment.



### Conclusion The effluent sample collected from the NPDES sampling point for Outfall D-004 for this facility on August 25, 2008, was not acutely toxic to either test spec ies during the 48-hour acute screening bioassays. Although the marine AGP result was less that the met hod detection limit (likely due to nutrient removal by the salts added during salinity adjustment), the effluent AGP result exceeded the "problem” threshold for fresh receiving waters suggesting that nutrient enrichment may be an issue. The effluent’s fecal coliform

### 3 bacteria concentration (590 CFU/100 ml) exceeded the water quality standard for fecal coliform bacteria in Class III marine waters ( ≤ 400 CFU/100 ml; 62-302.530(6), F.A.C.). Additional effluent water quality samples (not including metals or organic consti tuents) did not exceed any applicable water quality standards or violate any permit conditions.



### Literature Cited Miller, W. E., T. E. Maloney, and J. C. Greene. 1978. The Selenastrum capricornutum Printz algal assay bottle test. EPA-600/9-78-018. U. S. EPA, Cincinnati, Ohio. 126 p.

Manahan, S. E. 1994. Environmental Chemistry, 6 th edition. Lewis Publishers, USA. 811 pp

Raschke, R. L. and D. A. Schultz. 1987. The use of the algal growth potential test for data assessment. J. Wat. Poll. Cont. Fed. 59(4): 222-227.

### 4 Chevron Port Tampa Terminal
Inor g anic Total Location N:P ratio N:P ratio Effluent 19.4 1.5 ± 0.3 24.5 ± 4.9 76.2 ± 15.2 94.6 ± 18.9 0.7 9.1 Predicted AGP

(TP) ± 20% Table 1a. Measured and predicted algal growth potential (A GP; mg dry weight/L) for total soluble inorganic nitrogen (TSIN), orthophosphate (OP), total nitrogen (TN) and tota l phosphorus (TP) limitation of the freshwater species Pseudokirchneriella subcapitata for samples collected from the Chevron Port Tampa Terminal on August 25, 2008.

AGP (measured) Predicted AGP (TSIN) ± 20% Predicted AGP (OP) ± 20% Predicted AGP (TN) ± 20%

Chevron Port Tampa Terminal
Inor g anic Total Location N:P ratio N:P ratio Effluent* 3.0 U 0.7 9.1 * - sample salinity adjusted to 7ppt U - Material analyzed for but not detected; value reported is the method detection limit Table 1b. Measured algal growth potential (AGP; mg dry weight/L) of the saltwater species Dunaliella tertiolecta and ratios of nitrogen to phosphorus for samples collected from the Chevron Port Tampa Terminal on August 25, 2008.

AGP (measured)

### 5 Chevron Port Tampa Terminal NPDES# FL0035921 Class III Criteria Effluent Limits

Not Collected* - -

Aluminum ≤ 1,500 - - Arsenic ≤ 50 - - Cadmium ≤ 8.8 - - Copper ≤ 3.7 Report - Iron ≤ 300 Report - Lead ≤ 8.5 Report - Mercury ≤ 0.025 Report - Nickel ≤ 8.3 - - Selenium ≤ 71 - - Silver ≤ 2.3 - - Zinc ≤ 86 - -

Ortho-phosphate - - 0.057 Total Phosphorus - - 0.22 Total Ammonia - - 0.034 Un-ionized Ammonia - - ≤ 0.02 c Nitrate and Nitrite - - 0.006 I Total Kjeldahl Nitrogen - - 2 Organic Nitrogen - - 1.966 c Total Nitrogen - - 2.006 c

Dissolved Oxygen (mg/L) ≥ 4.0 s - 8.0 pH (SU) 6.5 - 8.5 s 6.5 - 8.5 s 8.3 Conductivity (μmhos/cm) - - 416 Temperature °C - - 32.4 Total Residual Chlorine (mg/L) ≤ 0.01 - 0.03 UQ£ Chlorophyll a (μg/L) - Corrected - - 26 Phaeophytin (μg/L) - - 6 Turbidity (NTU) < 29 t 12 CBOD, 5 day (mg/L), N - inhibited - - 5.9 Flow (MGD) - - 0.012 a Cyanide (μg/L) ≤ 1.0 Report 0.005 U Hardness (mg CaCO 3 ) - - 119 £

Fecal Coliforms ≤ 400s # - 590

Value exceeds the Class III Water Quality Criteria or permit limits a - Annual average c - Value is calculated s - Single sample t - Shall not exceed 29 NTUs above background I - The reported value is between the l aboratory method detection lim it and the laboratory pr actical quantitation limit Q - Sample held beyond normal holding time U - Material analyzed for but not detected; va lue reported is the me thod detection limit £ - Bioassay laboratory measurement * - Samples not collected due to pr iority event in Central Laboratroy # - If 10 or fewer samples are taken within a month, t he single sample limit is 400 CFU (62-302.530(6), F.A.C.) Nutrients (mg/L)

General Physical and Chemical Parameters

Microbiology (Number of colony forming units/100ml) Table 2. Effluent limits, Class III Criteria for predominantly marine waters and chemical data for samples collected from the Chevron Port Tampa Terminal on August 25, 2008.

Effluent
Organic Constituents (μg/L)

Metals (μg/L unless otherwise noted) Not Collected* -

### 6 Appendix A. Chain of Custody Form

### 7 Appendix B. Bench Sheets

### 8 Appendix B. (continued)

### 9 Appendix B. (continued)

### 10 Appendix B. (continued)

### 11 Appendix B. (continued)

### 12 Appendix B. (continued)

### 13 Appendix C. SRT Bench Sheet s and Statistical Analysis Florida Department of Environmental Protection Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - 48-hour Acute Toxicity Ceriodaphnia dubia (water flea) - Sodium Chloride (NaCl)

Date LC50 Mean LC50 Std Dev Lower Control Limit Upper Control Limit 02/13/07 2.39 2.39 0.17 2.05 2.54 03/13/07 1.92 2.18 0.18 1.82 2.55 04/03/07 2.37 2.20 0.18 1.83 2.57 05/08/07 1.78 2.18 0.21 1.77 2.59 05/29/07 1.69 2.03 0.23 1.70 2.60 06/06/07 2.28 2.16 0.22 1.71 2.60 07/03/07 1.99 2.14 0.22 1.70 2.58 07/31/07 1.94 2.14 0.22 1.71 2.58 09/04/07 1.95 2.13 0.22 1.70 2.57 10/02/07 2.04 2.11 0.21 1.68 2.53 10/30/07 2.35 2.11 0.21 1.68 2.54 12/04/07 2.13 2.09 0.20 1.67 2.51 01/22/08 1.92 2.08 0.20 1.66 2.50 02/05/08 2.25 2.08 0.20 1.68 2.48 03/04/08 2.15 2.08 0.20 1.68 2.49 04/01/08 2.50 2.11 0.22 1.68 2.55 05/06/08 2.19 2.11 0.22 1.68 2.55 06/03/08 1.91 2.10 0.22 1.66 2.54 07/08/08 2.21 2.11 0.22 1.67 2.55 08/05/08 2.07 2.10 0.22 1.67 2.54

Means are of the last 20 SRT tests. Lower Control Limit = mean - 2 * Std Dev CV = 10.32 Upper Control Limit = mean + 2 * Std Dev EPA reference 75th quartile CV = 29%



### Standard Reference Toxicant Data Ceriodaphnia dubia 48 hour LC 50

1.50 2.00 2.50 3.0002/13/0703/13/0704/03/0705/08/0705/29/0706/06/0707/03/0707/31/0709/04/0710/02/0710/30/0712/04/0701/22/0802/05/0803/04/0804/01/0805/06/0806/03/0807/08/0808/05/08



### Test Dateg/L NaCl

LC50 Mean LC50 Lower Control Limit Upper Control Limit

### 14 Appendix C. (continued) Florida Department of Environmental Protection Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - 48-hour Acute Toxicity C

y

prinella leedsi ( bannerfin shiner ) - Sodium Chloride ( NaCl )

Date LC50 Mean LC50 Std Dev Lower Control Limit Upper Control Limit 02/14/07 8.92 8.92 0.49 7.12 9.10 03/14/07 7.75 8.34 0.48 7.16 9.09 04/03/07 8.50 8.39 0.48 7.19 9.12 05/08/07 9.07 8.22 0.52 7.18 9.25 05/30/07 8.85 8.62 0.53 7.23 9.32 07/03/07 8.67 8.33 0.50 7.34 9.32 07/10/07 8.52 8.36 0.49 7.38 9.34 08/01/07 7.7 8.31 0.51 7.29 9.34 09/05/07 9.06 8.34 0.54 7.26 9.42 10/02/07 8.55 8.43 0.47 7.45 9.35 10/30/07 8.72 8.43 0.47 7.48 9.37 12/04/07 7.71 8.36 0.47 7.41 9.31 01/08/08 8.7 8.35 0.47 7.41 9.30 02/05/08 8.92 8.42 0.46 7.50 9.33 03/04/08 7.89 8.42 0.46 7.50 9.33 04/01/08 8.24 8.42 0.45 7.51 9.33 05/15/08 8.1 8.39 0.51 7.36 9.41 06/03/08 8.21 8.37 0.51 7.34 9.40 07/08/08 8.04 8.33 0.50 7.32 9.33 08/05/08 7.76 8.33 0.50 7.33 9.33

Means are of the last 20 SRT tests. Lower Confidence Limit = mean - 2 * Std Dev CV = 6.02 Upper Confidence Limit = mean + 2 * Std Dev EPA reference 75th quartile CV = 19%



### Standard Reference Toxicant Data Cyprinella leedsi 48-hour LC 50

4 6 8 1002/14/0703/14/0704/03/0705/08/0705/30/0707/03/0707/10/0708/01/0709/05/0710/02/0710/30/0712/04/0701/08/0802/05/0803/04/0804/01/0805/15/0806/03/0807/08/0808/05/08



### Test Dateg/L NaCl

LC50 Mean LC50 Lower Control Limit Upper Control Limit

### 15 Appendix C. (continued)

### 16 Appendix C. (continued)

### 17 Appendix C. (continued)

### 18 Appendix C. (continued)

### 19 Appendix C. (continued)



### Ceriodaphnia dubia Acute Standard Reference Toxicant Test

DATE: 8-5-08 DURATI ON: 48 hr TOXICANT : NaCl SPECIES: Ceriodaphnia dubia

RAW DATA: Concentration Number Mortalities (g/L) Exposed .00 20 0 1.50 20 1 2.00 20 6 2.50 20 20 3.00 20 20 4.00 20 20

SPEARMAN-KARBER TRIM: 5.00%

SPEARMAN-KARBER ESTIMATES: LC50: 2.07 95% LO WER CONFIDENCE: 1.95 95% UPPER CONFIDENCE : 2.20

_

___________________ ______________________ ________________ Cyprinella leedsi Acute Standard Refe rence Toxicant Test

DATE: 8-5-08 DURATI ON: 48 hr TOXICANT : NaCl SPECIES: Cyprinella leedsi

RAW DATA: Concentration Number Mortalities (g/L) Exposed .00 20 0 6.00 20 0 7.00 20 1 8.00 20 13 9.00 20 20 10.00 20 20

SPEARMAN-KARBER TRIM: .00%

SPEARMAN-KARBER ESTIMATES: LC50: 7.76 95% LOWE R CONFIDENCE: 7.53 95% UPPER CONFIDENCE: 8.00

### 20 Appendix D. Test Organism Receipt(s)

### 21 Appendix D. (continued)22Appendix E. Chemical Analyses performed on the effluent from the ChevronPort Tampa Terminal Outfall D-004,sampled on August 25, 2008.Date Sampled Field ID Analyses Component Result Remark Units MDL PQL8/25/2008 14:10 Effluent AGP/LN Algal Growth Potential 19.4 mg DryWt/L 0.3 0.98/25/2008 14:10 Effluent AGP/LN Algal Growth Potential-Salinity Adjusted 3 U mg DryWt/L 3 38/25/2008 14:10 Effluent Chlorophyll/Grain Size/BOD Chlorophyll-a, Corrected 26 ug/L 0.73 2.38/25/2008 14:10 Effluent Chlorophyll/Grain Size/BOD Biochemical Oxygen Demand-5 Day,N-Inhib 5.9 mg/L 0.2 28/25/2008 14:10 Effluent Chlorophyll/Grain Size/BOD Phaeophytin-a 6 ug/L 0.32 0.958/25/2008 14:10 Effluent Overflow Fecal Coliforms-Membrane Filter 590 #/100 mL 108/25/2008 14:10 Effluent Toxicology Bioassay-Acute-Screen-FW-C.dubia, LC50 100 L LC508/25/2008 14:10 Effluent Toxicology Bioassay-Acute-Screen-FW-Fish, LC50 100 L LC508/25/2008 14:10 Effluent Nutrients NO2NO3-N 0.006 I mg N/L 0.004 0.018/25/2008 14:10 Effluent Nutrients Kjeldahl Nitrogen 2 mg N/L 0.08 0.28/25/2008 14:10 Effluent Nutrients O-Phosphate-P 0.057 mg P/L 0.004 0.018/25/2008 14:10 Effluent Nutrients Total-P 0.22 mg P/L 0.02 0.068/25/2008 14:10 Effluent Nutrients Ammonia-N 0.034 mg N/L 0.01 0.028/25/2008 14:10 Effluent Nutrients Cyanide 0.005 U mg CN/L 0.005 0.028/25/2008 14:10 Effluent Nutrients Turbidity 12 NTU 0.1 0.18/25/2008 14:10 Effluent Field Parameter Specific Conductance 416 umhos/cm8/25/2008 14:10 Effluent Field Parameter Sample Depth 0.2 m8/25/2008 14:10 Effluent Field Parameter Temperature 32.42 C8/25/2008 14:10 Effluent Field Parameter Dissolved Oxygen 7.97 mg/L8/25/2008 14:10 Effluent Field Parameter pH 8.338/25/2008 13:55 Field Blank Chlorophyll/Grain Size/BOD Biochemical Oxygen Demand-5 Day,N-Inhib 0.2 U mg/L 0.2 28/25/2008 13:55 Field Blank Nutrients Kjeldahl Nitrogen 0.08 U mg N/L 0.08 0.28/25/2008 13:55 Field Blank Nutrients Ammonia-N 0.01 U mg N/L 0.01 0.028/25/2008 13:55 Field Blank Nutrients Total-P 0.02 U mg P/L 0.02 0.068/25/2008 13:55 Field Blank Nutrients Cyanide 0.005 U mg CN/L 0.005 0.028/25/2008 13:55 Field Blank Nutrients O-Phosphate-P 0.004 U mg P/L 0.004 0.018/25/2008 13:55 Field Blank Nutrients NO2NO3-N 0.004 U mg N/L 0.004 0.01

### 23 Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 3 5 9 2 1 11 12 0 8 2 5 0 8 18 B 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 3 5 9 2 1 11 12 0 8 2 5 0 8 18 X 19 S 20 2

66

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 3 5 9 2 1 11 12 0 8 2 5 0 8 18 S 19 S 20 2

66 

## The Bioassay of the Chevron Port Tampa Terminal effluent sampled on August 25, 2008. NPDES #FL0035921.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks



## The Priority Pollutants Analysis of the Chevron Port Tampa Terminal effluent sampled on August 25, 2008. NPDES #FL0035921.

Biological Analyses of the Chevron Port Tampa Terminal effluent sampled on August 25, 2008. NPDES #FL0035921.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks