Exhibit 31.1

CERTIFICATION

Pursuant
to the requirements of Rules 13a-14 of the Securities Exchange Act of 1934, as
amended, James R. Musick provides the following certification.

I,
James R. Musick, President, Chief Executive Officer and Principal Financial Officer of
Vitro  Diagnostics, Inc. (Company) certify that:

|1. |  | I
have reviewed this annual report on Form 10-KSB of the Company;
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|2. |  | Based
on my knowledge, this annual report does not contain any untrue statement of a  material
fact or omit to state a material fact necessary to make the statements  made, in light of
the circumstances under which such statements were made, not  misleading with respect to
the period covered by this annual report;
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|3. |  | Based
on my knowledge, the financial statements, and other financial information  included in
the annual report, fairly present in all material respects the  financial condition,
results of operations and cash flows of the Company as of,  and for, the periods
presented in this annual report;
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|4. |  | I
am responsible for establishing and maintaining disclosure controls and  procedures (as
defined in Exchange Act Rules 13a-14 and 15-d-14) for the  registrant and have designed
such disclosure controls and procedures to ensure  that material information relating to
the Company is made known to me by others,  particularly during the period in which this
annual report is being prepared;
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|5. |  | I
have disclosed, based on my most recent evaluation, to the Companys  auditors and
the audit committee of our board of directors (or persons  performing the equivalent
functions):
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|a. |  | All
significant deficiencies in the design or operation of internal controls which could
adversely affect the Companys ability to record, process, summarize and report
financial data and have identified for the Companys auditors any material
weaknesses  in internal controls, and
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|b. |  | Any
fraud, whether or not material, that involves management or other employees which have  a
significant role in the Companys internal controls; and
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|6. |  | I
have indicated in this annual report whether or not there were significant  changes in
internal control or in other factors that could significantly affect  internal controls
subsequent to the date of my most recent evaluation, including  any corrective actions
with regard to significant deficiencies and material  weaknesses.
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Date: February
9, 2007

| | /s/   
James R. MusickJames R. Musick, President, Chief Executive Officerand Principal Financial Officer
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