# Patent

## Title
Process and composition for therapeutic cisplatin (CDDP)

## Abstract

These pharmaceutical preparations are intended for the delivery of the spheroids they contain, and are characterized by the absence of an adverse effect on the release profile of the active principle(s) following a possible compression step.

## Background
The present invention relates to a novel pharmaceutical form, in the form of spheroids, containing one or more active principles, with the exception of tiagabine.

The invention also covers the process for the preparation of such spheroids and multiparticulate pharmaceutical preparations containing these spheroids. These pharmaceutical preparations are intended for the delivery of the spheroids they contain, and are characterized in that they do not adversely affect the release profile of the active principle(s) contained in the spheroids.

The term spheroids is understood to refer to spherical units whose size can range from 0.25 mm to 3 mm, preferably from 0.5 mm to 1 mm.

U.S. Pat. No. 4,684,516 describes tablets for oral administration which disintegrate rapidly in aqueous medium, which comprise coated granules capable of releasing an active principle at a controlled rate over several hours into the intestinal system and which comprise 2 to 15% by weight of binders and lubricants.

U.S. Pat. No. 4,684,516 describes in particular granules obtained by application of a layer of active principle to unsimilar materials, these granules then being coated with a first film containing a mixture of stearic acid, carnauba wax and talc, and a second film consisting of disintegrating agents--such as starch, cellulose or alginic acid--which also serve to provide the tablet's cohesion.

Patent application EP 468,436 describes prolonged-release tablets obtained by compression of an active principle and of a mixture of two powders, one being hydrophobic and the other water-soluble. The hydrophobic powder is obtained by melting and spraying a mixture of stearic acid, glycerol and hydrogenated castor oil. The water-soluble powder is a cellulose mixed with lactose.

Patent application EP 548,356 describes multi-particulate tablets which are rapidly broken down, comprising an active substance in the form of mono-crystals or microgranules.

These tablets are obtained by pregranulation of a mixture of excipients consisting of one or more breakdown agents of carboxymethylcellulose or polyvinylpyrrolidone type, one or more swelling agents of the starch type and a direct compression sugar such as dextrose. The microgranules or monocrystals are dry-mixed into the mixture of excipients before being tabletted.

It has been shown that the excipients usually used to coat non-tabletted granules, such as cellulose derivatives, cannot absorb the mechanical stresses to which the granules are subjected during compression (International Journal of Pharmaceutics, No. 143, 13-23, 1996).

The compression of coated granules is a delicate operation since it changes the structure of the coating film by causing fissures to appear or by rupture, which leads to the partial or total loss of the properties of the film.

Fissuration of the granules irreversibly changes the release profile of the active principle(s) they contain.

In order to conserve the characteristics of the film coating the granules after compression, the granules of the prior art are diluted with auxiliary substances whose role is to absorb the physical stresses associated with compression (binders) and to allow disintegration of the tablet (disintegrating agents) in liquid medium, i.e. in aqueous solution or in the digestive juices.

The tablet formulations of the prior art use auxiliary substances added to the granules during compression in order to avoid surface fissuration of these granules.

The subject of the present invention relates to spheroids containing one or more active principles with the exception of tiagabine, which can be compressed directly without the addition of a substantial part of an auxiliary substance, i.e. less than 5% by weight, preferably less than 1% by weight.

The subject of the present invention is spheroids containing one or more active principles with the exception of tiagabine, comprising: thermoplastic excipient which is of pasty to semi-solid consistency at a temperature of about 20.degree. C., and whose melting point is between about 25.degree. C. and about 100.degree. C., coated with a film whose glass transition temperature is less than about 30.degree. C., which ensures either protection or masking of the taste, or modified and controlled release of the active principle(s).

The core can be composed in particular of a mixture of sucrose and starch or microcrystalline cellulose.

In the context of the present invention, thermoplastic excipients is understood to refer to compounds having a melting point of between 25 and 100.degree. C. and characterized by a pasty to semi-solid consistency at temperature of about 20.degree. C.

The role of the thermoplastic excipients is, in particular, during a possible compression step, to allow the spheroids to deform plastically, and thus to absorb some of the stresses to which they are subjected, such that their surface is not fissured or torn.

The excipients can advantageously be chosen from partially hydrogenated oils, beeswax, carnauba wax, paraffin waxes, silicone waxes, C12-C18 fatty alcohols and fatty acids, solid, semi-synthetic glycerides, glycerol monoesters, diesters or triesters, polyoxyethylene glycols and glycosylated polyoxyethylenated glycerides, and mixtures thereof.

The layer containing at least one thermoplastic excipient is coated with a flexible and deformable film, comprising a polymer material, whose glass transition temperature is less than about 30.degree. C., preferably less than about 20.degree. C.

The polymer film comprises a polymer material which is either a polymer or a mixture of at least one polymer and a plasticizer.

The polymer film can serve, depending on the case, to protect the active principle from the environment (degradation by light, by ambient moisture), to mask the taste of the active principle or to modify its release (prolonged, delayed or programmed release).

The polymer is preferably an acrylic, vinyl or cellulose polymer or copolymer.

The term plasticizer is understood to refer to a product which lowers the glass transition temperature of the polymer.

The plasticizer is preferably chosen from triethyl citrate, acetyl triethyl citrate, tributyl citrate, acetyl tributyl citrate, triacetin, diethyl phthalate, polyethylene glycols, polysorbates, mono- and diacetylated glycerides, and mixtures thereof.

The mechanical properties of the polymer film, in particular the percentage of elongation and the breaking strength, can serve as selection criteria for the polymer and/or the plasticizer. These mechanical properties can also be determined by the method described in standards DIN53 455 and ISO/RI 184.

In the context of the present invention, a polymer or a polymer/plasticizer mixture having a percentage of elongation of greater than about 50% is preferably chosen, in order to coat spheroids intended to be compressed.

For example, the polymer Eudragit NE30D.RTM. sold by the company Rohm, which is a neutral copolymer of acrylic and methacrylic acid esters in the form of an aqueous 30% dispersion, has a percentage of elongation of 600% and a breaking strength equal to 8 N/mm.sup.2, which makes it particularly flexible and deformable.

According to a first variant of the invention, the active principle(s) is/are dispersed in the bulk of the core.

According to a second variant, the active principle(s) is(are) dispersed in the layer containing at least one thermoplastic excipient.

According to a third variant, the active principle(s) is(are) applied to the surface of the core and then coated with a layer containing at least one thermoplastic excipient.

The active principle can be protected, in one of the above four variants, by combining it with an antioxidant and/or by coating it with a protective film.

Lastly, according to a fourth and final variant, the active principle(s) is(are) dispersed in the bulk of the core and in the layer containing at least one thermoplastic excipient.

The spheroids according to the invention can advantageously be coated with a water-dispersible outer layer.

The outer layer provides the said spheroids with cohesion during a possible compression step, and ensures that the tablet obtained breaks down in aqueous medium.

The water-dispersible outer layer preferably consists of an acrylic, vinyl or cellulose polymer.

The subject of the present invention is also the process for the preparation of the spheroids described above.

The cores can be manufactured by assembly in a turbomixer from calibrated sucrose crystals, or by extrusion-spheronization.

When the cores are prepared by extrusion-spheronization, the tiagabine can be mixed into the extruded-spheronized bulk.

According to the preparation process of the invention, the layer containing at least one thermoplastic excipient, the film containing a polymer material, and optionally the outer protective layer, are successively deposited on the cores by spraying, in a granulating turbomixer, in a perforated turbomixer, in a fluidized-air bed or by any other appropriate means.

The process according to the invention comprises two or three steps depending on whether it is desired to prepare spheroids containing an outer layer which is water-dispersible, or otherwise.

The spheroids containing a water-dispersible outer layer are particularly suitable for the preparation of tablets.

The first step, known as the assembly step, consists in depositing the thermoplastic excipient(s) onto the cores. The assembly preparation can, depending on the case, be in the form of solid dispersions in aqueous or organic media, in the form of solutions, in the form of emulsions or in the molten state.

When the active principle is dispersed in the bulk of the layer containing at least one thermoplastic excipient, the active principle is incorporated into the assembly preparation.

According to another variant of the process according to the invention, the active principle can be bound by dusting onto the neutral cores premoistened with the assembly preparation.

The second step of the process according to the invention consists in depositing the polymer film which allows protection of the active principle, masking of the taste, or modified release of the active principle. The polymer coating preparation can be in the form of solutions or dispersions in aqueous or organic medium.

The third optional step of the process according to the invention consists in depositing the protective outer layer by spraying the coating preparation, which can be in the form of a solution or dispersion in aqueous or organic medium.

It is advantageously possible, as need be, to add an anti-adhesive agent such as talc, a plasticizer such as polyethylene glycol, and an antioxidant such as dl-.alpha.-tocopherol to the coating and assembly preparations.

It is advantageously possible to add a disintegrating agent to the protective outer layer so as to accelerate the release of the spheroids in aqueous medium, when these have been tabletted.

The disintegrating agent is, for example, cross-linked carboxymethylcellulose, crosslinked polyvinylpyrrolidone or sodium carboxymethyl starch.

The subject of the present invention is also multiparticulate pharmaceutical preparations containing the spheroids described above, which can be obtained by the process of the invention.

The multiparticulate pharmaceutical preparations according to the invention are preferably in the form of gelatin capsules filled with the said spheroids, or tablets of the said spheroids.

The said tablets are advantageously prepared without the substantial addition of auxiliary substances. Up to 5% by weight of a lubricant such as talc can be added to the spheroids before compression.

The said tablets advantageously comprise spheroids protected by a water-dispersible layer consisting of an acrylic, vinyl or cellulose polymer or a water-dispersible thermoplastic excipient, or any other excipient which is soluble in aqueous medium.

This layer has the role of ensuring cohesion between the spheroids, of thereby ensuring the hardness of the tablet, and of allowing the tablet to disintegrate when it is immersed in solution.

The tablets according to the invention are dispersible in solution and yield independent spheroids, such that the release profile of the tablet and of the spheroids which constitute it are virtually equivalent.

The reason for this is that the tablets according to the invention allow the delivery of spheroids without adversely affecting the release profile of the active principle(s) they contain under the effect of the compression.

The tablets according to the invention can be composed solely of spheroids according to the invention, or of a mixture of spheroids and of placebo spheroids, that is to say spheroids in accordance with the present invention but lacking the active principle.

The stresses exerted on the spheroids, during the compression step, can range from 5 kN to 50 kN, but preferably between 5 kN and 15 kN.

The hardness of the tablets is preferably between 10 N and 100 N, more preferably between 10 N and 50 N.

The disintegration time of the tablets in aqueous medium at 37.degree. C. is less than 60 min.

The tablets according to the invention preferably have a mass of between 0.1 g and 1 g.

They may be round, oval or oblong in shape, they may have a flat or concave surface and may have cleaving grooves or bars.

The tablets according to the invention can be subjected to a final protective or colouring coating operation.

The present invention will be described more particularly with the aid of the examples which follow and of the single figure, which should not be considered as limiting the invention.

The single figure represents the percentage by mass of in-vitro dissolution of a specific active principle, isosorbide mononitrate, for the non-compressed spheroids according to the invention (curve 1) and for a table of spheroids according to the invention (curve 2).

Assembly Step hydroxypropylmethylcelluose (Pharmacoat 603.RTM. sold by the company Shin Etsu), (sold by NP Pharm) in a perforated turbomixer, so as to obtain an envelope composed of Pharmacoat 603.RTM. and of active principle, Transfer the filler thus obtained into a conventional turbomixer; heat the filler to a temperature of about 50.degree. C., onto the hot rotated filler. Precirol.RTM. is a thermoplastic compound which, by softening and spreading on contact with the filler maintained at 50.degree. C., forms a uniform film around the cores. Precirol.RTM..

Coating Step

The filler obtained above is divided into two. Only part is coated: RS30D.RTM. (sold by the company Rohm), and of a plasticizer, Myvacet 9.45.RTM. (sold by the company Eastman Kodak), turbomixer,

Mixing Step the details of the compositions of the two batches and of the mixture are given below: Assembled Coated    Mixture Mixture Composition cores(g)  cores(g)  (g)   (%) ______________________________________ Neutre 26 .RTM. 25.4      59.2      84.6  56.1 Isosorbide 5.9       13.8      19.7  13.1 mononitrate (active principle) Pharmacoat 603 .RTM. 1.0       2.2       3.2   2.1 Precirol .RTM. 6.5       15.1      21.6  14.3 Talc       1.3       3.0       4.3   2.9 Eudragit RS30D .RTM. --        14.4      14.4  9.6 Myvacet 9.45 .RTM. --        2.9       2.9   1.9 Total      40.1      110.6     150.7 100.0 ______________________________________

Compression Step

The spheroid mixture is compressed on a Frogeras OA alternating press.

Results of In-vitro Dissolution

An in-vitro dissolution test is carried out on the tablets according to the method described in the pharmacopoeia (USP XXIII, <711>machine 2).

Conditions

The active principle is assayed by ultraviolet spectrophotometry.

The table below gives the percentage of active principle released by the spheroids before and after compression as a function of time. Spheroids    Dispersible tablets percentage by mass percentage by mass of isosorbide of isosorbide mononitrate released mononitrate released Time (h)   (%)          (%) ______________________________________ 0          0            0 1          28.2         34.8 4          62.2         63.2 8          86.4         80.9 ______________________________________

The results given in the single figure show that there is no significant difference between the dissolution profiles of the active principle before and after compression.

Assembly step

Preparation of the Assembly Suspension

Weigh out the excipients in the proportions given below. Materials    Amount (g)  Percentage ______________________________________ Codeine      100.0       52.1% of the dry extract Novata AB .RTM. wax 38.0        19.8% of the dry extract PEG-6000     13.0         6.8% of the dry extract Polysorbate 80 6.0          3.1% of the dry extract d1-.alpha.-Tocopherol 10.0         5.2% of the dry extract Talc         25.0        13.0% of the dry extract Purified water 233.0       100% of the solvent ______________________________________ Dry extract content: 45.2% homogeneous solution is obtained, (sold by Roche), and the oily solution in order to obtain an oil-in-water type emulsion, operation.

Assembly on Neutre Cores turbomixer, a perforated turbo-mixer or a fluidized-airbed, above, operation,

Sieve the mass of microgranules obtained.

Final formula: Materials          Percentage ______________________________________ Neutres 30 .RTM.   74.1% Codeine            13.5% Novata AB .RTM. wax 5.1% PEG-6000           1.8% Polysorbate 80     0.8% d1-.alpha.-Tocopherol 1.3% Talc               3.4% Theoretical codeine content 135 mg/g ______________________________________

Coating Step

1. Preparation of the Sustained-release Coating Suspension Materials      Amount (g)  Percentage ______________________________________ Eudragit NE 30D .RTM. 40.0 g     80.0% of dry extract PEG-6000        10.0 g     20.0% of dry extract Purified water 158.0 g     100% of solvent ______________________________________ Content of the dry extract: 24.0% until a homogeneous suspension is obtained,

Coating of the Assembled Spheroids a perforated turbomixer or a fluidized air bed, described above while maintaining the bed of spheroids at a temperature below 25.degree. C.,

2. Preparation of the Protective-coating Suspension Materials   Amount (g)    Percentage ______________________________________ Opadry OYB .RTM. 95.0          95.0% of dry extract PEG-6000    5.0           5.0% of dry extract Purified water 1000          100% of solvent ______________________________________ Content of the dry extract: 10.0% until a homogeneous suspension is obtained,

Coating of the Sustained-release Spheroids turbomixer or a fluidized-airbed, described above while maintaining the bed of spheroids at a temperature below 25.degree. C., the bulk thus obtained.

Final formula corresponding to 35% w/w of prolonged-release coating and 5% w/w of protective coating: Materials          Percentage ______________________________________ Neutres 30 .RTM.   44.9% Codeine            8.2% Novata AB .RTM. wax 3.1% PEG-6000           8.3% Polysorbate 80 .RTM. 0.5% d1-.alpha.-Tocopherol 0.8% Talc               2.1% Opadry OYB .RTM.   4.1% Eudragit NE 30D .RTM. 28.0% Theoretical codeine content 82 mg/g ______________________________________

The spheroids are then compressed on a Pette P1200 type rotary instrumented machine. The compression forces applied are between 10 kN and 30 kN.

Characteristics of the tablets measured on 10 units: Average Size 1 .times. L .times. h Hard-       Disinte- Dosage   weight  (mm)        ness Friability gration ______________________________________ 29.93 mg/g 365 mg  12.5 .times. 7.5 .times. 4.8 20 N <0.1%  45 min ______________________________________

Results of in vitro dissolution:

Conditions: <711>machine 2, revolutions/minute.

The active principle is assayed by HPLC at a wavelength of 285 nm.

The table below gives the percentage of active principle released by the spheroids before and after compression as a function of time. Spheroids     Dispersible tablets percentage of Codeine percentage of Codeine Time (h)  released      releaseed ______________________________________ 1         20.5          24.5 2         45.9          52.3 3         63.9          73.4 4         75.4          85.0 6         89.4          94.4 8         90.5          97.1 ______________________________________

Assembly Step

Preparation of the Assembly Suspension

Weigh out the excipients in the proportions indicated below Material        Amount (g) Percentage (%) ______________________________________ Morphine sulphate 183.54    19.77 Diethyl phthalate 27.57     2.97 Talc            13.76     1.48 Water           703.68    75.98 ______________________________________ Dry extract content: 24.22%

Procedure

Successively introduce purified water and then, with stirring, the active principle portionwise into a stainless steel container. With the stirring continued, gradually add the diethyl phthalate and then the talc.

Mix the suspension using an Ultra-Turrax.RTM. turbomixer.

Assembly on Neutre Cores

Place 153 g of Neutres 26.RTM. into a fluidized-air-bed perforated turbomixer or coating turbomixer.

Assemble the active principle by continuous spraying of the suspension described above.

Protective Coating

Weigh out the following excipients into a stainless steel container:

Application of the Mixture to the Granules (conventional turbomixer, turbomixer with fluidized air bed, perforated turbomixer). reached. of the fluidized air bed technology, by spraying the molten mixture.

Primary Coating Material         Amount (g) Percentage ______________________________________ Endragit NE 30D .RTM. 180.74  g     65.1 Talc             5.6     g     2.0 Water            91.3    g     32.9 ______________________________________ Dry extract content: 21.5% Ultra-Turrax.RTM. turbomixer. prepared above while maintaining, irrespective of the technique selected, a granule bed temperature of below 23.degree. C.

Secondary Coating Material         Amount (g) Percentage ______________________________________ Pharmacoat 603 .RTM. 14.4      7.4 PEG 400          1.5       0.77 Water            178.1     91.8 ______________________________________ water with stirring and continue stirring until dissolution is complete. prepared above.

Final Formula Material        Percentage by mass ______________________________________ Neutrea 26 .RTM. 30.28 Morphine sulphate 36.33 Diethyl phthalate 5.46 Talc            6.74 Precirol .RTM.  7.29 Eudragit NE 30D .RTM. 10.73 Pharmacoat 603 .RTM. 2.85 PEG 400         0.29 ______________________________________ Theoretical content of morphine sulphate: 235.67 mg/g

The spheroids are then compressed on a rotary instrumented machine such as Fette P1200.

The compression forces applied are between 10 KN and 30 KN.

Characteristics of the tablets obtained Average Dosage   weight      Size 1 .times. L .times. h Hardness ______________________________________ 99.8 mg/g 423.5 mg    12.5 .times. 7.5 .times. 5.2 19 ______________________________________

Results of in Vitro Dissolution

Conditions

Vane dissolution machine in accordance with standard (USP XXIII, <711>machine 2).

Medium 500 ml, purified water at 37.degree. C.

Stirring speed 100 rpm.

The active principle is continuously assayed by UV at wavelengths of: 285-320 nm. Spheroids      Tablets Percentage of morphine Percentage of morphine Time    sulphate released sulphate released ______________________________________ 0       0              0 1       8              13 2       32             30 3       51.4           46 4       64.5           65.2 6       79.8           90.2 8       88             96 ______________________________________

## Claims

1. Spheroids comprising one or more active principles, with the exception of tiagabine, wherein said spheroids further comprise: one thermoplastic excipient which is of pasty to semi-solid consistency at a temperature of about 20.degree. C., and whose melting point is between about 25.degree. C. and about 100.degree. C., coated with either protection or masking of the taste, or modified and controlled release of said one or more active principles, wherein said spheroids can be compressed directly without the addition of a substantial part of an auxiliary substance.

2. Spheroids according to claim 1, wherein said thermoplastic excipient is chosen from partially hydrogenated oils, beeswax, carnauba wax, paraffin waxes, silicone waxes, C12-C18 fatty alcohols and fatty acids, solid, semi-synthetic glycerides, glycerol monoesters, diesters or triesters, polyoxy-ethylene glycols and glycosylated polyoxyethylenated glycerides, and mixtures thereof.

3. Spheroids according to claim 2, wherein said glass transition temperature of said flexible and deformable film is less than about 20.degree. C.

4. Spheroids according to claim 1, wherein said polymer material is a polymer or a mixture of at least one polymer and a plasticizer.

5. Spheroids according to claim 4, wherein said plasticizer is chosen from triethyl citrate, acetyl triethyl citrate, tributyl citrate, acetyl tributyl citrate, triacetin, diethyl phthalate, polyethylene glycols, polysorbates, mono- and diacetylated glycerides, and mixtures thereof.

6. Spheroids according to claim 1, wherein said polymer material has a percentage of elongation of greater than about 50%.

7. Spheroids according to claim 1, wherein said spheroids are coated with a water-dispersible outer layer which provides said spheroids with cohesion during a compression step and which ensures breakdown in aqueous medium of the tablet obtained.

8. Spheroids according to claim 7, wherein said water-dispersible outer layer consists of an acrylic, vinyl or cellulose polymer.

9. Spheroids according to claim 1, wherein said one or more active principles are dispersed in the bulk of said core.

10. Spheroids according to claim 1, wherein said one or more active principles are dispersed in said layer containing at least one thermoplastic excipient.

11. Spheroids according to claim 1, wherein said one or more active principles are dispersed in the bulk of said core and in said layer containing at least one thermoplastic excipient.

12. Spheroids according to claim 1, wherein said one or more active principles are applied to the surface of said core and then coated with said layer containing at least one thermoplastic excipient.

13. A process for the preparation of the spheroids according to claim 1, wherein said layer containing at least one thermoplastic excipient and said film containing a polymer material are successively deposited on the cores by spraying, in a granulating turbomixer, in a perforated turbomixer, or in a fluidized-air bed.

14. Multiparticulate pharmaceutical preparations comprising said spheroids according to claim 1 and a pharmaceutically acceptable carrier.

15. The pharmaceutical preparations according to claim 14, wherein said pharmaceutical preparations are in the form of gelatin capsules filled with said spheroids.

16. The pharmaceutical preparations according to claim 14, wherein said pharmaceutical preparations are in the form of tablets comprising said spheroids.

17. The pharmaceutical preparations according to claim 16, wherein said tablets are prepared without the addition of a substantial amount of an auxiliary substance.

18. The pharmaceutical preparations according to claim 17, wherein said tablets are prepared by a compression step and wherein up to 5% by weight of talc is added to said spheroids before said compression step.

19. The spheroids of claim 1, wherein said auxiliary substance is less than 5% by weight of said spheroids.

20. The spheroids of claim 1, wherein said auxiliary substance is less than 1% by weight of said spheroids.

21. A process for the preparation of spheroids according to claim 7, wherein said layer containing at least one thermoplastic excipient, said film containing a polymer material, and said outer layer are successively deposited on the cores by spraying, in a granulating turbomixer, in a perforated turbomixer, or in a fluidized-air bed.

22. Spheroids according to claim 1, wherein said flexible and deformable film has a glass transition temperature of less than about 30.degree. C.