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 Chronic Aquatic Invertebrate Toxicity

CAS Number : 3194-55-6

Sponsored 

Chemical 

Name : 

Cyclododecane, 1,2,5,6,9,10-hexabromoTest Substance - Chronic Aquatic Invertebrate Toxicity

Test 

Substance: (25637-99-4) Cyclododecane, hexabromoTest Substance 

Purity/

Composition 

and Other Test 

Substance 

Comments: 

 Estimated by Calculation 

Test Substance 

Result Type: Estimated 

Method - Chronic Aquatic Invertebrate Toxicity

Year Study 

Performed: 1998 

Method/

Guideline 

Followed: 

 Other 

Other Method/

Guideline: EPA OTS 797.1330 

Deviations 

from Method/

Guideline: 

 

Species/In 

Vitro System: Daphnia magna 

GLP: Yes 

Analytical 

Monitoring: Yes 

Test Type: 

Test Vessel: 

Water Media 

Type: 

Test 

Concentrations: 

Nominal and 

Measured 

Concentrations: 

 Nominal test concentrations were 0.85, 1.7, 3.4, 6.8 and 13.6 ug HBCD/L water; dose levels were based on 

gamma HBCD's water solubility, 3.4 ug/L. Measured test concentrations (LC/MS with negative ion atmospheric 

pressure ionization) were 0.87, 1.6, 3.1, 5.6 and 11 ug HBCD/L water (based on the gamma stereoisomer). 

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Exposure 

Period: 21 Days 

Vehicle Used: 

Vehicle Name: 

Vehicle Amount 

and Units: 

Alkalinity: 

Dissolved 

Oxygen: 

pH Value: 

Test 

Temperature 

and Units: 

 

Photo (Light/

Dark): 

Salinity: 

TOC: 

Water 

Hardness: 

Method/

Guideline 

Test Conditions 

Remarks: 

 Endpoint: other: survival, reproduction and growth 

Daphnia magna Life Cycle (21 Day) (Sponsor: ACC Brominated Flame Retardant Industry Panel) 

The test article was a composite of 3 lots of commercial HBCD produced by Albemarle Corporation, Great Lakes 

Chemical Corporation, and Ameribrom, Inc. This study was performed according to current EPA, OECD and GLP 

guidelines. 

Limit Test: 

Test Results - Chronic Aquatic Invertebrate Toxicity

NOEC Exposure 

Duration: 

NOEC: = 3.4 Other 

LOEC Exposure 

Duration: 

LOEC: = 5.6 Other 

NOELR 

Exposure 

Duration: 

 

NOELR: 

LOELR 

Exposure 

Duration: 

 

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LOELR: 

LC/EC Mean 

Value: Exposure 

Duration

Exposure 

Units

LC/

EC

% Value 

Description

Mean 

Value 

or 

Lower 

Mean 

Value

Upper 

Mean 

Value

Units Effect 

Observed

Basis for 

Concentration

 %

Results 

Remarks: 

 No statistically significant effects on survival, reproduction or growth of Daphnia magna were seen at HBCD 

concentrations < 3.1 ug/L (measured). Thus, HBCD's no effect concentration (NOEC), based on survival, 

reproduction and growth, to daphnia magna for 21 days was equivalent to HBCD's water solubility. The measured 

NOEC in this study was 3.1 ug/L and corresponded to a nominal HBCD concentration of 3.4 ug/L, e.g. gamma 

HBCD's water solubility. The lowest observed effect concentration (LOEC) and the maximum acceptable toxicant 

concentration (MATC) based on survival, growth and reproduction were greater than HBCD's water solubility. The 

LOEC, 5.6 ug/L, corresponded to nominal concentrations twice gamma HBCD's water solubility. The effect seen at 

this dose level was a reduction in length. Survival and reproduction at the 5.6 ug/L dose level were not affected. 

The MATC, 4.2 ug/L, was calculated as the mean of the NOEC and the LOEC. The MATC was greater than gamma 

HBCD's water solubility. 

Reliability/Data Quality - Chronic Aquatic Invertebrate Toxicity

Reliability: Valid Without Restrictions 

Reliability 

Remarks: Critical study for SIDS endpoint 

Key Study 

Sponsor 

Indicator: 

 Key 

Reference - Chronic Aquatic Invertebrate Toxicity

Reference: Drottar K. and Krueger H. 1998. Hexabromocyclododecane (HBCD): Flow-through life-cycle toxicity test with the 

cladocema (Daphnia magna). Project No.: 439A-108. Wildlife International, Ltd. Easton, MD. 

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