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## Chronic Aquatic Invertebrate Toxicity

CAS Number : 3194-55-6 Sponsored Chemical Name : Cyclododecane, 1,2,5,6,9,10-hexabromo
### Test Substance - Chronic Aquatic Invertebrate Toxicity
Test Substance: (25637-99-4) Cyclododecane, hexabromoTest Substance Purity/ Composition and Other Test Substance Comments: Estimated by Calculation

Test Substance Result Type: Estimated 

### Method - Chronic Aquatic Invertebrate Toxicity Year Study Performed: 1998

Method/ Guideline Followed: Other

Other Method/ Guideline: EPA OTS 797.1330

Deviations from Method/ Guideline: Species/In Vitro System: Daphnia magna

GLP: Yes Analytical Monitoring: Yes
Test Type:
Test Vessel:
Water Media Type:
Test Concentrations:
Nominal and Measured Concentrations: Nominal test concentrations were 0.85, 1.7, 3.4, 6.8 and 13.6 ug HBCD/L water; dose levels were based on gamma HBCD's water solubility, 3.4 ug/L. Measured test concentrations (LC/MS with negative ion atmospheric pressure ionization) were 0.87, 1.6, 3.1, 5.6 and 11 ug HBCD/L water (based on the gamma stereoisomer).

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Exposure Period: 21 Days

Vehicle Used:
Vehicle Name:
Vehicle Amount and Units: Alkalinity:
Dissolved Oxygen: pH Value:
Test Temperature and Units: Photo (Light/ Dark): Salinity:
TOC:
Water Hardness:
Method/ Guideline Test Conditions Remarks: Endpoint: other: survival, reproduction and growth Daphnia magna Life Cycle (21 Day) (Sponsor: ACC Brominated Flame Retardant Industry Panel) The test article was a composite of 3 lots of commercial HBCD produced by Albemarle Corporation, Great Lakes Chemical Corporation, and Ameribrom, Inc. This study was performed according to current EPA, OECD and GLP guidelines.

Limit Test: 

### Test Results - Chronic Aquatic Invertebrate Toxicity NOEC Exposure Duration:
NOEC: = 3.4 Other LOEC Exposure Duration:
LOEC: = 5.6 Other
NOELR Exposure Duration:
NOELR:
LOELR Exposure Duration:

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LOELR:
LC/EC Mean Value: Exposure Duration Exposure Units LC/ EC % Value Description Mean Value or Lower Mean Value Upper Mean Value Units Effect Observed Basis for Concentration

%

Results Remarks: No statistically significant effects on survival, reproduction or growth of Daphnia magna were seen at HBCD concentrations < 3.1 ug/L (measured). Thus, HBCD's no effect concentration (NOEC), based on survival, reproduction and growth, to daphnia magna for 21 days was equivalent to HBCD's water solubility. The measured NOEC in this study was 3.1 ug/L and corresponded to a nominal HBCD concentration of 3.4 ug/L, e.g. gamma HBCD's water solubility. The lowest observed effect concentration (LOEC) and the maximum acceptable toxicant concentration (MATC) based on survival, growth and reproduction were greater than HBCD's water solubility. The LOEC, 5.6 ug/L, corresponded to nominal concentrations twice gamma HBCD's water solubility. The effect seen at this dose level was a reduction in length. Survival and reproduction at the 5.6 ug/L dose level were not affected. The MATC, 4.2 ug/L, was calculated as the mean of the NOEC and the LOEC. The MATC was greater than gamma HBCD's water solubility. 

### Reliability/Data Quality - Chronic Aquatic Invertebrate Toxicity Reliability: Valid Without Restrictions Reliability Remarks: Critical study for SIDS endpoint
Key Study Sponsor Indicator: Key


### Reference - Chronic Aquatic Invertebrate Toxicity
Reference: Drottar K. and Krueger H. 1998. Hexabromocyclododecane (HBCD): Flow-through life-cycle toxicity test with the cladocema (Daphnia magna). Project No.: 439A-108. Wildlife International, Ltd. Easton, MD.

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