Interlaboratory Analytical Comparison 

Study to Support Deepwater Horizon 

Natural Resource Damage Assessment: 

Description and Results for Crude Oil 

QA10OIL01

Michele M. Schantz

John R. Kucklick

NISTIR 7793
2

NISTIR 7793

Interlaboratory Analytical Comparison Study to Support 

Deepwater Horizon Natural Resource Damage 

Assessment: Description and Results for Crude Oil

QA10OIL01

Michele M. Schantz and John R. Kucklick

Analytical Chemistry Division

Material Measurement Laboratory

National Institute of Standards and Technology

Gaithersburg, MD 20899 and Charleston, SC 29412

September 2011

U.S. Department of Commerce

Rebecca M. Blank, Acting Secretary

National Institute of Standards and Technology

Patrick D. Gallagher, Under Secretary for Standards and Technology and Director
3

ABSTRACT

To support natural resource damage assessment (NRDA) in response to the Deepwater Horizon (DWH) 

oil spill in the Gulf of Mexico, a large number of coastal sediment and tissue (i.e., oysters) samples 

have been collected outside of the spill zone to define baseline environmental conditions prior to being 

exposed to oil. Analysis of oiled sediments and oil-exposed oysters will continue for the foreseeable 

future. To support these efforts, NOAA will require additional analytical laboratories to perform

NRDA sample analyses. To compare the data among these laboratories, inter-laboratory comparison 

studies have been initiated with the results from the second exercise, crude oil QA10OIL01 reported 

here. In this exercise, selected polycyclic aromatic hydrocarbons (PAHs), alkylated PAHs, hopanes, 

and steranes were determined in the exercise material, which consisted of a crude oil, and in SRM 1582

Petroleum Crude Oil. The results from this second exercise are reported along with a summary of the 

analytical methods used. 

INTRODUCTION

On April 20, 2010, a fatal explosion, fire, and sinking of BP’s Deepwater Horizon drilling rig occurred 

approximately 40 miles off the Louisiana coast. The disaster resulted in the discharge of tens of 

thousands of barrels of oil per day from the seafloor into the Gulf of Mexico. In what has become the 

worst offshore oil spill in U.S. history, a wide expanse and variety of natural resources have become 

exposed and potentially impacted by oil and other consequences of the spill. Under the Oil Pollution 

Act, those responsible for an oil spill are liable for clean-up and for natural resource damages. Several 

federal and state agencies are conducting a natural resource damage assessment (NRDA) to determine

what resources have been injured and what uses of the resources have been lost due to the spill. 

To support this NRDA, the trustees and BP’s representatives have been collecting and analyzing tens of 

thousands of environmental samples to characterize both pre-spill and post-spill environmental 

conditions. A broad range of sample types have been collected including oil in various forms, water,

sediment, and biota. For the foreseeable future, subsequent sampling and analysis will be required. In 

addition, numerous other entities have collected environmental samples for hydrocarbon analysis and 

submitted them to different laboratories throughout the country. 

In the past the National Institute of Standards and Technology (NIST) has helped benchmark and 

improve the quality of analytical data gathered on the marine environment by administering 

interlaboratory comparison exercises. To compare the data among the many laboratories analyzing 

samples from this spill, the National Oceanic and Atmospheric Administration (NOAA) has requested 

that NIST coordinate interlaboratory comparison studies with sediment, crude oil, and bivalve tissue

being the three matrices of interest. These studies are performance-based with each laboratory using its 

current methods for analysis of similar matrices that it would use for its program customers. The target 

analytes for each study are selected polycyclic aromatic hydrocarbons (PAHs), alkylated PAHs, 

hopanes, and steranes. More than three dozen laboratory facilities were contacted by NOAA and 

invited to participate in the studies; for all three matrix studies a large number of laboratories agreed to 

receive samples and report their analytical results to NIST.
4

The data received from 26 laboratories for the crude oil QA10OIL01 are summarized in this report 

along with summaries of the analytical methods used by each laboratory. Numerical indices, z- and pscores, are used to assess and track laboratory performances for accuracy and precision, respectively, 

and to provide a mechanism for assessing the comparability of data produced by the participating 

laboratories for the target analytes. 

SOURCE OF MATERIAL 

The crude oil used for the exercise source oil was collected under NOAA’s direction from the site of 

the Deepwater Horizon oil rig on May 21, 2010 (one month after the incident), stored at another 

laboratory, and then shipped to NIST (Gaithersburg, MD) on September 22, 2010. On October 18, 

2010, the contents of six of the containers received were divided among five separatory funnels and 

allowed to sit for at least one hour until the water and oil separated. The water was then removed and 

discarded while the oil was allowed to sit for another hour. In all cases, no additional water separated 

from the oil. The oil was then combined in a 20-L glass carboy containing a large stir bar. The glass 

carboy was covered with a black trash bag and stirred overnight. The following day the crude oil was 

ampouled. Aliquots of approximately 1.3 mL of oil were placed in 2 mL amber ampoules. The 

ampoules were filled with argon just prior to filling with the solution. A total of 66 gross were 

produced with ampoules taken from boxes 64 and 65 for distribution in this study. The remainder of 

the ampoules will be used for SRM 2779 Gulf of Mexico Crude Oil following the certification process. 

SAMPLE DISTRIBUTION

Five ampules were distributed to each of 37 laboratories during the week of November 29, 2010. Each 

laboratory was requested to analyze three samples of Crude Oil QA10OIL01 and at least one or more 

samples of SRM 1582 Petroleum Crude Oil [2] using their laboratory's and/or program's analytical 

protocols, for determination of the concentrations (mass/mass) of the parent polycyclic aromatic 

hydrocarbon (PAH) compounds, alkylated PAH compounds, hopanes, and steranes currently being 

determined in their laboratory. Note that SRM 1582 was not provided to the laboratories. 

The instructions including the list of target analytes sent to participants are attached in Appendix A.

EVALUATION OF EXERCISE RESULTS 

Establishment of the Assigned Values

Laboratory data submission: Each participating laboratory was asked to submit data from three 

replicate determinations of the “unknown” material QA10OIL01 and was requested to report results of 

concurrent analyses of NIST SRM 1582 Petroleum Crude Oil. Laboratories were requested to report 

these results to three significant figures and to provide brief descriptions of their cleanup and analytical 

procedures. 

Determination of laboratory analyte means: For each laboratory, the laboratory analyte mean of the 

three sample results (S1, S2, and S3) was calculated for each analyte. Non-numerical data were treated 
5

as follows: A mean "<value" was used when three "<values" were reported; NA (not 

analyzed/determined) was used for three reported NAs; and, if the reported results were of mixed type, 

e.g., S1 and S2 were numerical values and S3 was reported as “<value”, the two similar "types" were 

used to either determine the mean or to set a non-numerical descriptor.

Determination of assigned values: The assigned values are the means of the acceptable data as defined 

here. For a particular analyte, the performance on the reference material, SRM 1582, was initially 

deemed acceptable for the purpose of this exercise if the laboratory result was within 30 % of the upper 

and lower limits of the confidence interval for analytes listed in the Certificate of Analysis for SRM 

1582 [2]. The criterion of 30 % is the same as the one that was used for the National Oceanic and 

Atmospheric Administration (NOAA) Mussel Watch and National Status and Trends Quality 

Assurance Programs [3]. If a laboratory demonstrated acceptable performance on a particular analyte

in the reference material, that laboratory’s results for that analyte in the corresponding “unknown” 

exercise material was then used in the calculation of the analyte’s exercise assigned value, unless it was 

deemed an outlier. For evaluation of potential outliers, statistical tests and expert analyst judgment 

were used after viewing both normal and log-normal plots of the data. This judgment utilized 

knowledge of potential coeluters based on the laboratory's reported methods. In instances for which the 

analyte concentration was below the detection limit of most participating laboratories, no exercise 

assigned value was calculated. In data sets where a number of laboratories report results as "not 

detected" at various detection limits, there is no consensus as to what numerical value should be 

assigned to these results in the computation of consensus means and other values.

REPORTED RESULTS

Laboratories were assigned numerical identification codes in order of receipt of data with the exception 

of the two NIST laboratories which are Lab 1 and 25 in this exercise. The laboratory mean replicate 

data are shown in Tables 1 to 3 for QA10OIL01. Included in these tables are the exercise assigned 

mean values and the standard deviation of the assigned mean values along with the exercise assigned 

median values. Summaries of the methods used by each laboratory are in Appendix B, and notes 

included by a laboratory with its data are listed in Appendix C. In Appendix D, charts of the mean 

numerical results reported by each laboratory for each analyte are shown for the exercise material and 

the corresponding reference material, SRM 1582. 

Performance Scores

The exercise coordinators recognize that different environmental monitoring programs have different 

data quality objectives and needs. The acceptability of the results submitted by a particular laboratory 

will be decided by the individual program(s) for which the laboratory provides data. Typically, each 

program will use these exercise results in conjunction with the laboratory's performance in the analysis 

of certified reference materials and/or control materials, and of other quality assurance samples. These 

exercise results are exhibited in a number of ways in this report to facilitate their use by most 

environmental monitoring programs in their acceptability assessments. 

IUPAC guidelines describe the use of z-scores and p-scores for assessment of accuracy and precision in 

intercomparison exercises such as those described in this report. These indices assess the difference 

between the result of the laboratory and the exercise assigned value and can be used to compare the 
6

performance of different laboratories among the participants on different analytes and on different 

materials.

Accuracy Assessment (z-score)

z-score = (bias estimate)/(performance criterion) = (x - X)/ σ

where x is the individual laboratory result, X is the "Exercise Assigned Value,” and σ is the target value 

for standard deviation. 

The choice of σ is dependent upon data quality objectives of a particular program, or as ISO 17043 

suggests “a fitness for purpose goal for performance as determined by expert judgement” [4]. It can be 

"fixed" and arrived at by perception, prescription, or referenced to validated methodology (e.g., σ = 

0.025 X; X is the exercise assigned value,), or it can be an estimate of the actual variation (e.g., the 

calculated sample standard deviation, s, from the exercise data). The "fixed" performance criterion is 

more useful in the comparison of a laboratory's performance on different materials while the use of the 

actual variation may be more useful within a given exercise, for example, if the determination of a 

particular analyte is exceptionally problematic. The measurement of analytes targeted in this study is

not particularly problematic.

 

We have calculated and reported z-scores using the fixed performance criterion for each analyte for 

each laboratory using "25 % of the exercise assigned value" as the fixed target value for standard 

deviation for this program. The use of z-score (25 % X) is also taken from the NOAA Mussel Watch 

and National Status and Trends Quality Assurance Programs [3]. The z-scores calculated for these 

exercises can thus be interpreted as shown in the following examples:

z-score (25 % X):

+1  laboratory result is 25 % higher than the assigned value

-2  laboratory result is 50 % lower than the assigned value.

A common classification of z-scores is [4]:

z  2 Satisfactory

2 < z < 3 Questionable

z  3 Unsatisfactory.

This classification has been deemed acceptable within the measurement community. 

Tables 4 through 6 summarize the z-scores (25 %) for each laboratory for each reported analyte in 

QA10OIL01. 

Precision Assessment (p-score)

p-score = σlab / σtarget

For the calculation of p-scores for this program, the σ values used are coefficients of variation (CV 

calculated as relative standard deviations) with the current target σ (CV) for the three replicates being 
7

15 %. Tables 7 through 9 summarize the relative standard deviations (RSDs) calculated from the three 

concentrations reported by the laboratory for each analyte quantified while Tables 10 through 12

summarize the p-scores (15%). A p-score of 1 indicates that the laboratory’s CV was 15%, and a pscore of 2 indicates that the laboratory’s CV was 30%.

 

DISCUSSION

NOAA’s NRDA office solicited laboratories involved in the analysis of samples shortly after the DWH 

disaster for their interest in participating in this interlaboratory study for analytes of interest in crude 

oil. The participation by the laboratories was voluntary, and samples of QA10OIL01 were provided 

free of charge. Laboratories were provided with information for ordering SRM 1582 (See Appendix 

A). Interested laboratories were requested to quantify selected PAHs, alkylated PAHs (some individual 

and some as groups), and biomarkers (hopanes and steranes) in three aliquots of QA10OIL01 and SRM 

1582 using their laboratories’ analytical protocols for these analyses. A total of 37 laboratories 

received samples of which 26 laboratories submitted data. The 26 participating laboratories are listed 

in alphabetical order in Appendix E. 

Tables 1 through 3 summarize the laboratory means and exercise assigned values for the PAHs, 

alkylated PAHs, and hopanes and steranes, respectively. The consensus value for a given compound in 

QA10OIL01 was derived by combining data where corresponding values in SRM 1582 were within 30

% of the expanded uncertainty of the SRM value. In the absence of a corresponding SRM value, 

individual results were screened using outlier tests and included in the consensus value if values were 

shown not to be statistical outliers. Appendix D contains the charts of the QA10OIL01 and SRM 1582

data by analyte. In these charts, the analytes that are not included on the Certificate of Analysis for 

SRM 1582 are shown with no target value. 

No assigned values were calculated for chrysene, triphenylene, benzo[j]fluoranthene, or 

benzo[k]fluoranthene in QA10OIL01. All of the data sets, except the two from NIST, were determined 

from gas chromatographic analyses on a 5% phenyl phase from different manufacturers. Chrysene and 

triphenylene coelute when using this phase as do several of the benzofluoranthene isomers. Although 

several laboratories recognized and reported coelutions for chrysene with triphenylene and among the

benzofluoranthenes using this phase, several did not, thus reporting the combined value for chrysene 

and triphenylene as only the chrysene peak and in a similar fashion for the benzofluoranthenes. Lab 25

(NIST) reported values for chrysene (mean 20.7 ng/g, std dev 0.4 ng/g, n=3) and triphenylene (mean 

14.4 ng/g, std dev 0.9 ng/g, n=3) in QA10OIL01 partially separated using a 50% phenyl phase while 

both Lab 1 (NIST) and Lab 25 (NIST) reported individual values for benzo[b], benzo[j], and 

benzo[k]fluroanthene separated on the 50% phenyl phase. Lab 1 (NIST) did not get enough separation 

of chrysene from triphenylene to report separate values for those compounds.

The spread in the PAH data for QA10OIL01 among the laboratories is highest for anthracene, perylene, 

benzothiophene, and naphthobenzothiophene, analytes at relatively low mass fractions and determined 

by fewer laboratories. The range in mass fractions for the PAHs in the crude oil QA10OIL01 is wide 

ranging from < 1 ng/g for perylene to > 800 ng/g for naphthalene. The agreement among the data for 

the more volatile PAHs, naphthalene and biphenyl, was better in the crude oil than in the previous 

sediment study [2] suggesting that extraction and sample concentration may be issues for the more 
8

volatile species. This will be further tested in the next study using a mussel tissue as the matrix of 

interest.

The alkylated PAH data for QA10OIL01 do not show as much spread among the laboratories as for the 

sediment material QA10SED01 [1]. However, similar to the sediment study, the laboratories reporting

1-methylnaphthalene and 2- methylnaphthalene as well as C1-naphthalenes, generally reported values 

for C1-naphthalenes lower than the sum of the 1-methyl- and 2-methylnaphthalene (see Figure 1). 

Labs 1, 9, 13, 20, and 23 reported very similar values for C1-naphthalenes and 1-methyl plus 2-

methylnapthalene. These labs, except for Lab 20, reported using the methylnaphthalenes to quantify 

the C-1 naphthalenes. Lab 23 also reported using the methylnaphthalenes to quantify the C1-

naphthalenes but their reported mass fraction for the C1-naphthalenes was 17% lower than the sum of 

the methylnaphthalenes. Lab 20, as well as most of the other laboratories, reported using naphthalene 

to quantify the C1-napthalenes. There are obviously issues with the choice of the representative 

compound used for quantification of the alkylated PAHs, as well as the interpretation of the 

chromatograms as to what peaks should and should not be included in the summation for the alkylated 

groups. For an alkylated group of PAHs, the mass spectra are different based on where the PAH is 

alkylated (or if it is alkylated) thus resulting in different response factors for isomers in a homolog 

group. These issues need to be explored in more detail in future studies and discussions. 

Fewer laboratories reported data for the selected biomarkers with the largest spread in the data seen for 

5α(H),14α(H),17α(H)-Cholestane 20S and 5α(H),14α(H),17α(H)-Cholestane 20R. 

Laboratories 1, 4, 9, 11, 16, 18, 23, 24, 25, and 26 performed some clean-up/isolation step for the oil 

samples (Appendix B) while the remaining laboratories diluted the oil samples and ran them on the 

GC/MS (without a clean-up/isolation step). Comparison of the means and standard deviations for 

selected PAHs (Figure 2) and selected steranes (Figure 3) for the laboratories performing a clean-up 

step to those for laboratories not performing a clean-up step shows that the means for the laboratories 

not performing a clean-up step are consistently higher than those for laboratories performing a clean-up 

step. The differences between the two groups of laboratories, however, are not greater than the spread 

of the data (as indicated by the standard deviations of the means). The consistently higher means may 

indicate that the laboratories not performing a clean-up step had a higher background interference not 

seen in the calibration solutions. 

The two crude oil samples, QA10OIL01 and SRM 1582, have different patterns of the PAHs and 

biomarkers. Using the data from Lab 1 (NIST) for illustration, Figure 4 shows that the mass fractions 

of the more volatile PAHs (molecular mass 128 through 192) are higher in QA10OIL01 as are the 

majority of the less volatile PAHs (molecular mass 202 through 276) in Figure 5. The notable 

exception is perylene which is almost 30 times higher in SRM 1582 compared to QA10OIL01. The 

hopanes and steranes (Figure 6) are higher in SRM 1582 compared to QA10OIL01. 

The majority of the z-scores based on 25% (Tables 4 through 6) are within ± 2 while the majority of the 

p-scores based on 15% (Tables 10 through 12) are within ± 1. This indicates that the laboratories are 

internally consistent, but there is still a fair amount of spread in the data among the laboratories. 

It is important to evaluate the non-quantitative results reported by each laboratory as well. Although 

these results are not easily presented or numerically evaluated, they are included in Tables 1 through 3 

of this report. The laboratory and its data users should closely examine these non-quantitative results. 
9

Decisions based on false negative or false positive results from a laboratory can lead to significant 

environmental and/or economic consequences. Some laboratories reported detection limits in the 

exercise material that may be too high for the data quality objectives and needs of their program(s), and 

these issues should be assessed as well.

Intercomparison exercises provide an important mechanism for assessing the comparability, 

repeatability, and trueness of data being produced by the participating laboratories. Exercise materials 

similar in matrix, form, and analyte concentration to typical samples routinely analyzed by the 

laboratories are most useful for demonstrating the level of comparability and for revealing potential 

problem areas. 

Minimizing the among-laboratory biases so that the analytical variability is significantly less than the 

field sampling variability should be an achievable goal in environmental monitoring.

Acknowledgments

The time and effort of the analysts and management of the participating laboratories and the assistance 

of NOAA with obtaining the crude oil and the NIST Standard Reference Materials Program with the 

preparation of the exercise material are gratefully acknowledged. 

Disclaimer

Certain commercial equipment, instruments, or materials are identified in this report to specify 

adequately the experimental procedure. Such identification does not imply recommendation or 

endorsement by the National Institute of Standards and Technology, nor does it imply that the materials 

or equipment identified are the best available for the purpose.

References

1. Schantz, M.M. and Kucklick, J.R., Interlaboratory Analytical Comparisons Study to Support 

Deepwater Horizon Natural Resource Damage Assessment: Description and Results for Marine 

Sediment QA10SED01, NISTIR ???, Gaithersburg, MD (2011).

2. Certificate of Analysis for Standard Reference Material (SRM) 1582 Petroleum Crude Oil, 

National Institute of Standards and Technology (NIST), Gaithersburg, MD, 1984. (https://wwws.nist.gov/srmors/view_detail.cfm?srm=1582)

3. Schantz, M.M., Parris, R.M., and Wise, S.A., NIST Intercomparison Exercise Program for 

Organic Contaminants in the Marine Environment: Description and Results of 2007 Organic 

Intercomparison Exercises, NISTIR 7501, Gaithersburg, MD (2008).

4. ISO/IEC 17043: 2010 Conformity Assessment-General Requirements for Proficiency Testing

5. IUPAC “The International Harmonized Protocol for the Proficiency Testing of (Chemical) 

Analytical Laboratories,” Pure & Appl. Chem., 65 (9), 2123-2144 (1993).
10

0

500

1000

1500

2000

2500

3000

3500

1 3 4 5 8 9 10 12 13 14 15 16 17 18 19 20 21 22 23 24 26

Lab

Mass fraction (ug/g)

sum 1-and 2-me

C1-naphthalenes

Figure 1. Comparison of data submitted by laboratory for the sum of individual values for 1-methyl naphthalene and 2-methylnaphthalene 

and for the C1-naphthalenes.
11

0.0

10.0

20.0

30.0

40.0

50.0

60.0

anthracene fluoranthene pyrene benzo[e]pyrene dibenzothiophene

mass fraction (ug/g)

clean-up

no clean-up

Figure 2. Comparison of mean mass fractions (and standard deviations) for selected PAHs determined by laboratories performing clean-up 

step (n=10) and laboratories not performing clean-up step (n=16) for QA10OIL01 
12

0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

80.0

5a(H),14a(H),17a(H)-Cholestane

20R

5a(H),14a(H),17a(H)-24-

Ethylcholestane 20R

5a(H),14b(H),17b(H)-Cholestane

20R

5a(H),14b(H),17b(H)-24-

Ethylcholestane 20R

mass fraction (ug/g)

clean-up

no clean-up

Figure 3. Comparison of mean mass fractions (and standard deviations) for selected steranes determined by laboratories performing clean-up 

step (n=10) and laboratories not performing clean-up step (n=16) for QA10OIL01 
13

0

200

400

600

800

1000

1200

1400

naphthalene 1-methylnaphthalene fluorene dibenzothiophene phenathrene 1-methylphenanthrene

ug/g oil

QA10OIL01

SRM 1582

Figure 4. Comparison of mass fractions (and standard deviations) for selected PAHs (molecular mass 128 to 192) determined by Lab 1

(NIST) in QA10OIL01 and SRM 1582 
14

0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

45.0

50.0

pyrene B[a]A triph+chry B[b]F B[e]P B[a]P perylene B[ghi]P

ug/g oil

QA10OIL01

SRM 1582

Figure 5. Comparison of mass fractions (and standard deviations) for selected PAHs (molecular mass 202 to 276) determined by Lab 1

(NIST) in QA10OIL01 and SRM 1582 
15

0

50

100

150

200

250

300

350

400

17a-22,29,30-trisnorh 17a,21b-30-norh 17a,21b-hopane 5a,14a,17a-chol 20R 5a,14b,17b-chol 20R

ug/g oil

QA10OIL01

SRM 1582

Figure 6. Comparison of mass fractions (and standard deviations) for selected hopanes and steranes determined by Lab 1 (NIST) in 

QA10OIL01 and SRM 1582
16

Table 1. Crude Oil (QA10OIL01): Laboratory means of three replicates and exercise assigned values - PAHs (μg/g)

 (reported as if three figures were significant)

Laboratory No. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

naphthalene 839 979 756 869 790 1260 746 851 855 749 653 778 1057 874 792

biphenyl 203 178 172 217 226 165 181 181 152 158 199 220 204 184

acenaphthene <5 <500 9.69 <4 DL 10.3 10.4 12.0 12.4 16.9 13.6 13.9 85.6 19.6 <9.35

acenaphthylene <5 <500 6.85 <4 DL 13.7 16.5 6.44 14.3 <10 8.50 7.47 13.8 7.47 5.44

fluorene 119 168 124 162 143 189 91.2 130 149 139 116 149 169 113 150

phenanthrene 259 311 277 304 273 363 271.3 262 284 263 247 320 378 286 301

anthracene 3.97 <500 13.0 7.52 DL DL 5.14 2.41 16.7 <10 10.7 15.0 22.8 11.9 15.7

fluoranthene 4.23 <500 4.44 <4 DL 4.95 6.05 4.96 6.24 <10 3.43 3.49 7.73 4.63 <9.35

pyrene 14.8 <500 14.7 9.33 DL 17.7 24.0 15.3 19.3 11.4 15.9 17.7 16.3 17.2 13.7

benzo[b]fluorene NA 10.9 22.6 NA NA NA NA 11.6 NA NA 11.5 NA NA

benz[a]anthracene 7.87 <500 4.20 15.3 DL 9.20 5.25 5.46 13.5 <10 6.73 5.94 6.14 9.06 <9.35

chrysene below <500 36.4 49.5 51.0 27.7 47.5 coelute 27.5 62.0 64.4 50.4 51.8

triphenylene below NA 49.5 NA NA NA NA NA OTHER 36 NA NA

chrysene / triphenylene 45.9 48.4 53.9 42.7

benzo[b ]fluoranthene 5.69 <500 7.59 5.36 7.83 6.52 8.08 5.26 5.71 <10 Other 5.89 6.82 5.83 <9.35

benzo[j]fluoranthene 0.602 NA <4 NA NA NA coelute Other OTHER 2.92 NA NA

benzo[k ]fluoranthene 0.503 <500 DL <4 DL DL ND <5.00 NA <1.2 < 3.89 3 <3.92 <9.35

Benzo[b+j]fluoranthene 3.63

benzo[j+k]fluoranthene 1.27 <10

benzo[a]fluoranthene <1 NA <4 NA NA NA NA <10 NA NA N/A NA NA

benzo[e]pyrene 10.3 10.6 10.1 13.0 15.1 12.2 11.2 12.3 <10 8.67 11.1 6.34 11.5 <9.35

benzo[a]pyrene 0.951 <500 DL <4 DL 3.04 ND 2.12 1.86 <10 <1.2 2.16 2.10 2.65 <9.35

perylene 0.751 DL <4 DL 1.88 ND <5.00 0.518 <10 1.27 0.67 0.69 <3.92 <9.35

indeno[1,2,3-cd]pyrene 0.419 <500 DL <4 DL DL ND <5.00 <0.478 <10 <1.2 < 1.94 215 <3.92 <9.35

benzo[ghi]perylene 2.45 <500 DL <4 DL 3.39 ND <5.00 2.04 <10 1.57 1.84 4.98 <3.92 <9.35

dibenz[a,h]anthracene 0.492 <500 3.30 <4 DL 2.76 ND 1.50 <10 2.17 1.73 N/A 2.17 <9.35

dibenz[a,c]anthracene 1.99

dibenz[a,h+ a,c]anthracene 2.26

cis/trans-decalin NA 790 814 NA NA NA NA 699 NA 704 1317 NA NA

cis-decalin

trans-decalin

dibenzofuran NA <500 27.5 24.7 NA 18.3 25.3 NA 35.4 NA 31.6 36.9 19.9 18.0

retene NA NA 19.8 NA NA NA 10.6 <10 20.9 68.5 13.4 NA NA

benzothiophene NA 6.88 9.03 NA NA NA NA <10 NA 8.3 22.2 NA NA

dibenzothiophene 54.8 52.8 41.2 58.3 42.4 44.9 51.5 55.1 49.7 42.2 59.7 51.3 44.4 54.3

naphthobenzothiophene NA 15.9 13.3 NA NA NA 32.5 NA 8.44 2.25 NA NA

benzo[b]naphtho[2,1-d]thiophene 19.5

benzo[b]naphtho[1,2-d]thiophene 3.51

benzo[b]naphtho[2,3-d]thiophene 2.65

NA=Not analyzed; DL=Detection limit; No value in space=nothing reported by lab: Bolded values were not used in the calculation of the consensus statistics
17

Table 1 (cont.). Crude Oil (QA10OIL01): Laboratory means of three replicates and exercise assigned values - PAHs (μg/g)

 (reported as if three figures were significant)

Laboratory No. 16 17 18 19 20 21 22 23 24 25 26 mean s %RSD median

naphthalene 767 751 801 743 866 657 769 843 953 919 843 837 126 15.0 820

biphenyl 176 279 202 164 204 168 161 168 284 223 177 194 34 17.5 181

acenaphthene <conc 32.9 12.1 33.1 16.4 17.0 22.2 14.0 DL 21.2 8.03 16.4 7.2 43.8 14.0

acenaphthylene <conc 34.1 <15 15.7 10.0 ND 6.86 <5.8 DL 8.08 DL 10.1 3.8 38.2 8.29

fluorene 112 267 117 141 153 119 132 164 106 217 120 140 28 20.3 139

phenanthrene 231 45.7 211 232 304 244 285 279 340 218 273 281 42 14.8 277

anthracene <conc <10 6.55 <0.02 <10 ND <1.99 7.34 8.66 2.85 4.33 8.79 4.79 54.5 7.52

fluoranthene <conc 5.33 4.29 11.0 6.50 ND 4.41 3.40 DL 4.10 2.13 4.72 1.36 28.7 4.44

pyrene <conc 17.0 11.7 11.4 20.8 10.6 18.3 12.7 14.6 14.4 17.0 15.5 3.5 22.4 15.3

benzo[b]fluorene <conc <10 14.4 13.8 NA NA 12.1 NA DL NA 22.0 14.9 4.7 31.8 13.0

benz[a]anthracene <conc 15.85 7.48 <0.02 8.60 10.5 6.27 5.06 DL 5.85 11.3 8.40 3.47 41.3 7.48

chrysene 63.1 22.3 ceolute 58.2 46.9 37.3 20.7 45.3 No assigned value

triphenylene NA <10 ceolute NA NA NA NA 14.4 NA No assigned value

chrysene / triphenylene 45.1 41.7 49.9 54.3 47.7 4.7 9.9 47.2

benzo[b ]fluoranthene <conc 5.78 4.44 ceolute 8.17 6.57 5.16 4.86 NA 6.15 4.83 6.13 1.13 18.4 5.83

benzo[j]fluoranthene NA 2.04 <0.02 NA NA NA NA 1.11 NA No assigned value

benzo[k ]fluoranthene <conc 2.57 <0.02 <10 NA <0.8 1.06 DL No assigned value

Benzo[b+j]fluoranthene No assigned value

benzo[j+k]fluoranthene 0.520 <1.99 NA No assigned value

benzo[a]fluoranthene NA <10 <0.50 ceolute NA NA <1.99 NA DL 1.96 DL No assigned value

benzo[e]pyrene <conc 16.5 10.4 12.0 13.7 11.4 10.2 10.6 DL 11.8 10.7 11.7 1.8 15.6 11.3

benzo[a]pyrene <conc <10 1.97 <0.02 <10 ND 2.24 <0.6 DL 1.42 DL 1.94 0.50 25.5 2.10

perylene <conc <10 <0.50 <0.02 <10 ND 1.03 0.697 DL 0.543 0.333 0.838 0.450 53.8 0.694

indeno[1,2,3-cd]pyrene <conc <10 <0.50 <0.02 <10 ND <1.99 <0.2 DL 0.617 DL No assigned value

benzo[ghi]perylene <conc <10 1.84 2.01 <10 ND 1.97 1.68 DL 2.10 2.20 2.10 0.49 23.4 2.01

dibenz[a,h]anthracene <conc <10 3.09 <0.02 <10 ND 2.50 1.48 DL 0.67 2.03 No assigned value 2.10

dibenz[a,c]anthracene No assigned value 1.99

dibenz[a,h+ a,c]anthracene No assigned value 2.26

cis/trans-decalin NA 614 632 NA NA 703 NA Other NA 1433 856 302 35.3 704

cis-decalin <0.02 No assigned value

trans-decalin 1270 No assigned value 1270

dibenzofuran <conc <10 <30 <0.02 29.4 NA 25.7 NA 28.3 NA 25.7 26.7 5.9 22.0 25.7

retene NA NA 7.37 <0.02 NA NA <1.99 NA 20.5 24.5 DL 16.7 6.3 37.6 19.8

benzothiophene NA <10 10.3 <0.02 NA NA 7.29 NA 23.0 NA 21.7 13.6 7.3 53.6 9.68

dibenzothiophene 41.7 <10 34.4 54.0 59.6 42.9 49.3 46.6 48.4 106 49.3 49.1 6.6 13.5 49.3

naphthobenzothiophene NA <10 16.7 3.09 NA NA 21.6 NA NA NA 17.0 12.3 7.0 56.9 14.6

benzo[b]naphtho[2,1-d]thiophene No assigned value

benzo[b]naphtho[1,2-d]thiophene No assigned value

benzo[b]naphtho[2,3-d]thiophene No assigned value

NA=Not analyzed; DL=Detection limit; No value in space=nothing reported by lab: Bolded values were not used in the calculation of the consensus statistics
18

Table 2. Crude Oil (QA10OIL01): Laboratory means of three replicates and exercise assigned values - alkylated PAHs (μg/g)

 (reported as if three figures were significant)

Laboratory No. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

1-methylnaphthalene 1138 1017 991 1100 1410 772 1103 1070 1025 1093 1177 1060 1177 1133

2-methylnaphthalene 1607 1877 1653 1820 1633 2107 1080 1767 1890 1477 1440 1813 1723 1513 1907

2,6-dimethylnaphthalene 1125 1017 1033 NA 1303 420 1042 1028 971 NA 1163 221 1160 993

1,6,7-trimethylnaphthalene NA 251 NA NA 471 111 306 212 174 NA 302 NA 334 487

1-methylphenanthrene 164 201 96.7 NA 218 180 180 195 106 NA 224 129 206 173

C1-decalins NA NA 1293 NA NA NA NA 1013 NA 1050 NA NA NA

C2-decalins NA NA 1743 NA NA NA NA 838 NA 928 NA NA NA

C3-decalins NA NA 1230 NA NA NA NA 513 NA 435 NA NA NA

C4-decalins NA NA 793 NA NA NA NA 481 NA 563 NA NA NA

C1-naphthalenes 2745 1417 1763 1833 NA 1717 2960 1597 NA 1793 2783 1520 1870

C2-naphthalenes 2250 2080 1917 2367 NA 1380 2047 3687 2000 4183 2237 3143 1823 2173

C3-naphthalenes 1503 1177 1130 1733 NA 787 1370 2990 1327 2460 1563 2200 1177 1397

C4-naphthalenes 776 515 489 840 NA 403 729 1360 698 1197 769 318 543 670

C1-benzothiophenes NA NA 53.9 NA NA NA NA 28.4 NA 33.7 212 NA NA

C2-benzothiophenes NA NA 35.6 NA NA NA NA 27.3 NA 40.8 4593 NA NA

C3-benzothiophenes NA NA 47.4 NA NA NA NA 36.6 NA 51.5 380 NA NA

C4-benzothiophenes NA NA 31.8 NA NA NA NA 25.0 NA 30.6 NA NA NA

C1-fluorenes 317 234 299 377 NA 174 298 320 300 252 327 704 242 286

C2-fluorenes 400 348 311 507 NA 180 396 408 409 341 444 NA 323 364

C3-fluorenes 266 244 256 413 NA 123 285 336 340 269 333 545 249 260

C1-phenanthrenes/anthracenes 971 630 638 700 NA 616 599 927 514 769 758 917 550 667

C2-phenanthrenes/anthracenes 789 603 632 807 NA 573 624 950 573 826 799 839 567 640

C3-phenanthrenes/anthracenes 488 309 355 497 NA 387 416 670 368 543 487 196 371 430

C4-phenanthrenes/anthracenes 286 171 184 197 NA 138 230 327 203 807 198 87.9 205 212

C1-dibenzothiophenes 135 152 132 97.3 NA 145 85.7 162 132 133 178 7.07 88.4 97.8

C2-dibenzothiophenes 120 189 153 143 NA 187 116 241 199 186 253 107 118 142

C3-dibenzothiophenes 102 139 115 123 NA 118 89.0 162 147 NA 181 87.9 85.9 102

C4-dibenzothiophenes 46.8 55.4 54.5 72.3 NA 46.6 83.8 65.7 NA 82.9 46.6 41.3 58.0

C1-fluoranthenes/pyrenes 60.6 65.4 67.8 99.0 NA 65.0 84.9 83.0 86.8 66.7 89.6 52.8 68.8 55.3

C2-fluoranthenes/pyrenes 125 117 90.4 167 NA 98.4 138 138 140 116 148 NA 125 121

C3-fluoranthenes/pyrenes 148 105 75.8 157 NA 82.6 135 159 137 124 165 65.9 113 111

C4-fluoranthenes/pyrenes 103 NA 60.5 NA NA NA 109 110 90.7 NA 7.63 NA NA

C1-naphthobenzothiophenes NA NA 54.5 NA NA NA 71.0 82.3 NA 30.4 NA NA NA

C2-naphthobenzothiophenes NA NA 57.2 NA NA NA 82.8 103 NA 37.0 NA NA NA

C3-naphthobenzothiophenes NA NA 41.5 NA NA NA 60.1 59.3 NA 25.2 NA NA NA

C4-naphthobenzothiophenes NA NA 21.2 NA NA NA 32.1 28.1 NA 11.3 NA NA NA

C1-chrysenes 107 85.9 108 113 NA 96.4 108 118 94.2 NA 134 179 95.9 110

C2-chrysenes 127 92.5 128 123 NA 115 117 143 114 NA 155 17.1 109 134

C3-chrysenes 85.5 63.9 94.8 103 NA 55.4 84.1 98.5 91.1 NA 114 NA 84.4 74.6

C4-chrysenes 70.9 39.3 39.1 NA NA ND 71.2 72.1 49.6 NA 75.2 3.98 48.3 <8.22

NA=Not analyzed; DL=Detection limit; No value in space=nothing reported by lab: Bolded values were not used in the calculation of the consensus statistics
19

Table 2 (cont.). Crude Oil (QA10OIL01): Laboratory means of three replicates and exercise assigned values - alkylated PAHs (μg/g)

 (reported as if three figures were significant)

Laboratory No. 16 17 18 19 20 21 22 23 24 25 26 mean s %RSD median

1-methylnaphthalene 992 2115 1063 1013 1183 989 1153 1124 1457 NA 1100 1102 137 12.5 1100

2-methylnaphthalene 1547 1078 1527 1563 1950 1585 1710 1621 1123 NA 1567 1623 259 15.9 1621

2,6-dimethylnaphthalene <conc 276 961 1016 1297 788 958 979 779 625 1000 916 293 32.0 997

1,6,7-trimethylnaphthalene NA N/A 389 306 370 286 193 NA 547 NA 377 320 119 37.2 306

1-methylphenanthrene 138 197 92.7 170 253 158 118 178 174 163 103 166 43 26.1 173

C1-decalins NA <10 1033 <0.02 NA NA 1018 NA Other NA 2000 1235 390 31.6 1042

C2-decalins NA <10 1200 <0.02 NA NA 817 NA DL NA 1800 1221 448 36.7 1064

C3-decalins NA <10 1000 <0.02 NA NA 384 NA DL NA 1433 833 449 53.9 757

C4-decalins NA <10 807 <0.02 NA NA 365 NA DL NA 1167 696 289 41.5 678

C1-naphthalenes 1640 2461 1700 2580 3137 2573 1827 2745 2150 NA 1600 2115 544 25.7 1833

C2-naphthalenes 2043 3800 2200 3687 2273 1044 1777 3476 1943 NA 1867 2409 830 34.5 2173

C3-naphthalenes 1297 8228 1300 2557 1447 615 1143 2482 1677 NA 1233 1571 604 38.5 1383

C4-naphthalenes 537 740 710 564 702 332 528 811 1593 NA 550 712 308 43.3 698

C1-benzothiophenes NA 74.9 32.3 <0.02 NA NA 29.0 NA NA NA 28.7 40.1 17.8 44.3 32.3

C2-benzothiophenes NA 66.6 29.3 <0.02 NA NA 26.9 NA NA NA 36.0 37.5 13.8 36.8 35.6

C3-benzothiophenes NA 368 30.7 <0.02 NA NA 42.5 NA NA NA 23.0 No assigned value

C4-benzothiophenes NA <10 30.0 <0.02 NA NA 31.4 NA NA NA 23.7 28.8 3.5 12.2 30.3

C1-fluorenes 244 763 247 554 308 260 276 320 326 NA 217 332 146 43.9 299

C2-fluorenes 297 57.1 360 503 476 353 347 399 419 NA 277 384 64 16.8 380

C3-fluorenes 218 130 283 167 364 276 251 185 280 NA 220 280 87 31.2 267

C1-phenanthrenes/anthracenes 506 1167 480 1257 710 613 614 872 750 NA 587 731 204 27.9 667

C2-phenanthrenes/anthracenes 509 9719 497 492 742 427 588 919 726 NA 617 670 146 21.7 628

C3-phenanthrenes/anthracenes <conc 1069 307 398 472 278 321 431 568 NA 347 411 109 26.5 398

C4-phenanthrenes/anthracenes <conc 49.1 207 51.2 256 166 139 142 322 NA 177 195 69 35.4 197

C1-dibenzothiophenes 70.7 219 103 75.7 101 153 138 130 93.7 NA 137 125 36 28.7 132

C2-dibenzothiophenes 75.0 12.8 160 91.6 139 208 167 174 122 NA 203 159 47 29.5 156

C3-dibenzothiophenes <conc 108 120 <0.02 114 83.2 121 102 89.7 NA 140 117 26 22.7 114

C4-dibenzothiophenes <conc 21.6 60.3 <0.02 106 NA 60.6 27.9 45.4 NA 56.7 57.4 20.2 35.3 56.0

C1-fluoranthenes/pyrenes <conc 343 52.7 195 97.1 98.3 81.7 62.5 51.3 NA 66.7 72.8 15.9 21.8 67.2

C2-fluoranthenes/pyrenes <conc <10 100 195 211 NA 138 115 87.0 NA 110 130 33 25.0 125

C3-fluoranthenes/pyrenes <conc 369 120 168 183 NA 156 101 102 NA 120 126 33 25.9 122

C4-fluoranthenes/pyrenes NA 72.7 82.7 175 NA NA 122 53.9 83.3 NA 77.3 95.0 32.5 34.2 87.0

C1-naphthobenzothiophenes NA 12.3 47.7 30.0 NA NA 62.5 NA NA NA 59.7 54.8 18.4 33.6 57.1

C2-naphthobenzothiophenes NA <10 67.3 41.3 NA NA 86.4 NA NA NA 72.0 68.4 22.7 33.2 69.7

C3-naphthobenzothiophenes NA 30.9 49.0 <0.02 NA NA 63.5 NA NA NA 46.7 47.0 14.0 29.7 47.8

C4-naphthobenzothiophenes NA <10 30.7 <0.02 NA NA 43.3 NA NA NA 35.7 28.9 10.3 35.6 30.7

C1-chrysenes 72.4 244 96.7 133 130 130 116 106 112 NA 94.7 108 16 15.1 108

C2-chrysenes <conc 364 130 150 163 162 144 115 140 NA 113 130 19 14.7 128

C3-chrysenes <conc <10 94.3 127 118 11.2 140 39.4 96.7 NA 79.0 91.3 24.7 27.1 92.7

C4-chrysenes <conc <10 77.0 <0.02 87.8 ND 79.6 7.56 70.7 NA 69.7 65.4 15.8 24.2 70.9

NA=Not analyzed; DL=Detection limit; No value in space=nothing reported by lab: Bolded values were not used in the calculation of the consensus statistics
20

Table 3. Crude Oil (QA10OIL01): Laboratory means of three replicates and exercise assigned values - biomarkers (μg/g)

 (reported as if three figures were significant)

Laboratory No. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Carbazole NA <500 NA 6.48 NA NA NA NA NA Other NA 8.88 NA NA

18a(H)-22,29,30-Trisnorhopane NA 5.17 NA 6.27 NA 5.30 NA NA Other 8.10 N/A 7.08 8.55

17a(H)-22,29,30-Trisnorhopane 6.88 4.87 NA 11.8 NA 5.03 NA NA Other 5.61 26.8 5.10 8.96

17α(H),21β(H)-30-Norhopane 13.9 13.0 NA 15.9 NA 12.7 NA NA Other < 5.82 N/A 15.3 1.69

18a(H)-30-Norneohopane NA 4.54 NA 10.4 NA 4.66 NA NA Other 17.0 N/A 4.93 5.58

17a(H)-Diahopane NA 3.28 NA 4.90 NA 4.26 NA NA Other 5.38 N/A 4.47 1.67

17α(H),21β(H)-Hopane 47.4 32.2 49.2 53.3 NA 29.0 NA NA Other 38.7 44.6 36.9 42.6

17α(H),21β(H)-22R-Homohopane 11.5 8.64 NA 17.0 NA 12.6 NA NA Other 10.3 N/A 10.7 11.1

17α(H),21β(H)-22S-Homohopane 15.3 14.0 NA 17.3 NA 8.89 NA NA Other 15.5 N/A 15.4 12.6

13b(H)17a(H)-Diacholestane 20S NA 50.1 NA 52.7 NA 35.4 NA NA Other 44.5 N/A 41.2 33.6

5a(H),14a(H),17a(H)-Cholestane 

20S NA 56.5 NA NA NA 43.0 NA NA Other 52.7 N/A 47.3 19.2

5a(H),14a(H),17a(H)-Cholestane 

20R 24.5 62.7 NA 75.7 NA 20.9 NA NA Other 54.3 34.9 55.3 20.0

5a(H),14a(H),17a(H)-24-

Ethylcholestane 20S NA 27.5 NA 28.0 NA 19.5 NA NA Other 19.8 N/A 30.0 <9.35

5a(H),14a(H),17a(H)-24-

Ethylcholestane 20R 19.6 22.6 NA 34.3 NA 41.5 NA NA Other 18.1 14.1 20.1 <9.35

5a(H),14b(H),17b(H)-Cholestane 

20R 24.7 24.6 NA 32.3 NA 15.4 NA NA Other 25.3 N/A 17.3 28.0

5a(H),14b(H),17b(H)-Cholestane 

20S NA 24.2 NA 36.7 NA 16.1 NA NA Other 24.6 N/A 18.4 18.5

5a(H),14b(H),17b(H)-24-

Ethylcholestane 20R 25.5 24.1 NA 34.0 NA 18.8 NA NA Other 30.7 N/A 24.9 16.8

5a(H),14b(H),17b(H)-24-

Ethylcholestane 20S NA 23.6 NA 33.0 NA 15.1 NA NA Other 23.1 N/A 19.8 31.1

NA=Not analyzed; DL=Detection limit; No value in space=nothing reported by lab: Bolded values were not used in the calculation of the consensus statistics
21

Table 3 (cont). Crude Oil (QA10OIL01): Laboratory means of three replicates and exercise assigned values - biomarkers (μg/g)

 (reported as if three figures were significant)

Laboratory No. 16 17 18 19 20 21 22 23 24 25 26 mean s %RSD median

Carbazole NA 11.4 <3.5 <0.02 NA ND 6.02 NA NA NA 8.19 2.48 30.2 7.68

18a(H)-22,29,30-Trisnorhopane <conc NA NA <10 NA 8.54 6.78 NA NA 6.97 1.35 19.4 6.93

17a(H)-22,29,30-Trisnorhopane <conc 22.0 NA <10 NA 7.08 7.39 7.93 NA 7.07 2.16 30.5 6.98

17α(H),21β(H)-30-Norhopane <conc NA 64.2 25.6 NA 18.4 17.9 21.6 NA 17.1 4.3 24.9 15.9

18a(H)-30-Norneohopane <conc NA NA <10 NA 7.47 7.59 NA NA 6.46 2.17 33.5 5.58

17a(H)-Diahopane <conc NA NA <10 NA 5.37 NA NA NA 4.19 1.33 31.6 4.47

17α(H),21β(H)-Hopane <conc NA 59.2 NA 57.9 NA 45.8 42.7 33.8 NA 43.8 9.2 21.0 43.6

17α(H),21β(H)-22R-Homohopane <conc NA NA 14.4 NA 14.9 12.1 17.6 NA 12.8 2.8 22.2 12.1

17α(H),21β(H)-22S-Homohopane <conc NA NA 18.8 NA 18.0 14.8 22.0 NA 15.7 3.4 21.9 15.4

13b(H)17a(H)-Diacholestane 20S <conc NA NA 78.8 NA 38.4 NA NA NA 42.3 7.2 17.1 41.2

5a(H),14a(H),17a(H)-Cholestane 

20S <conc NA NA 94.9 NA 46.5 11.9 NA NA 46.5 25.2 54.1 46.9

5a(H),14a(H),17a(H)-Cholestane 

20R <conc 59.9 NA 86.2 NA 50.5 21.5 10.7 NA 44.4 23.9 53.8 50.5

5a(H),14a(H),17a(H)-24-

Ethylcholestane 20S <conc NA NA 48.9 NA 24.9 10.3 NA NA 26.1 11.2 42.8 26.2

5a(H),14a(H),17a(H)-24-

Ethylcholestane 20R <conc NA NA 35.3 NA 18.0 14.0 12.5 NA 23.6 9.9 41.9 19.9

5a(H),14b(H),17b(H)-Cholestane 

20R <conc NA NA 45.5 NA 22.3 23.5 21.6 NA 25.5 8.1 31.7 24.6

5a(H),14b(H),17b(H)-Cholestane 

20S <conc NA NA 45.0 NA 22.3 17.9 NA NA 24.9 9.7 39.2 22.3

5a(H),14b(H),17b(H)-24-

Ethylcholestane 20R <conc 18.8 NA 49.9 NA 30.3 24.6 13.5 NA 26.0 9.7 37.2 24.8

5a(H),14b(H),17b(H)-24-

Ethylcholestane 20S <conc <10 NA 41.9 NA 20.6 20.3 NA NA 25.4 8.4 32.9 23.1

NA=Not analyzed; DL=Detection limit; No value in space=nothing reported by lab: Bolded values were not used in the calculation of the consensus statistics
22

Table 4. Crude Oil (QA10OIL01): z scores (25% by laboratory)- PAHs 

(z=+1 is 25% higher than the exercise assigned value; z=-1 is 25% lower than the exercise assigned value.)

Laboratory No. 1 2 3 4 5 6 7 8 9 10 11 12 13

naphthalene 0.0 0.7 -0.4 0.2 -0.2 2.0 -0.4 0.1 0.1 -0.4 -0.9 -0.3 1.1

biphenyl 0.2 -0.3 -0.5 0.5 0.7 -0.6 -0.3 -0.3 -0.9 -0.7 0.1 0.6

acenaphthene -1.6 -1.5 -1.5 -1.1 -1.0 0.1 -0.7 -0.6 16.8

acenaphthylene -1.3 1.4 2.6 -1.4 1.7 -0.6 -1.0 1.5

fluorene -0.6 0.8 -0.4 0.7 0.1 1.4 -1.4 -0.3 0.3 0.0 -0.7 0.3 0.8

phenanthrene -0.3 0.4 -0.1 0.3 -0.1 1.2 -0.1 -0.3 0.0 -0.2 -0.5 0.6 1.4

anthracene -2.2 1.9 -0.6 -1.7 -2.9 3.6 0.9 2.8 6.4

fluoranthene -0.4 -0.2 0.2 1.1 0.2 1.3 -1.1 -1.0 2.5

pyrene -0.2 -0.2 -1.6 0.6 2.2 -0.1 1.0 -1.1 0.1 0.6 0.2

benzo[b]fluorene -1.1 2.1 -0.9 -0.9

benz[a]anthracene -0.3 -2.0 3.3 0.4 -1.5 -1.4 2.4 -0.8 -1.2 -1.1

chrysene

triphenylene

benzo[b ]fluoranthene -0.3 1.0 -0.5 1.1 0.3 1.3 -0.6 -0.3 -0.2 0.4

benzo[j ]fluoranthene

benzo[k ]fluoranthene

benzo[a]fluoranthene

benzo[e]pyrene -0.5 -0.4 -0.5 0.5 1.2 0.2 -0.2 0.2 -1.0 -0.2 -1.8

benzo[a]pyrene -2.0 2.3 0.4 -0.2 0.5 0.3

perylene -0.4 5.0 -1.5 2.0 -0.8 -0.7

indeno[1,2,3-cd]pyrene

benzo[ghi]perylene 0.7 2.5 -0.1 -1.0 -0.5 5.5

dibenz[a,h]anthracene

cis/trans-decalin -0.3 -0.2 -0.7 -0.7 2.2

dibenzofuran 0.1 -0.3 -1.3 -0.2 1.3 0.7 1.5

retene 0.7 -1.5 1.0 12.4 -0.8

benzothiophene -2.0 -1.3 -1.6 2.5

dibenzothiophene 0.5 0.3 -0.6 0.8 -0.5 -0.3 0.2 0.5 0.1 -0.6 0.9 0.2

naphthobenzothiophene 1.2 0.3 6.6 -1.3 -3.3

chrysene + triphenylene -0.2 0.5 -0.4
23

Table 4 (cont). Crude Oil (QA10OIL01): z scores (25% by laboratory)- PAHs 

(z=+1 is 25% higher than the exercise assigned value; z=-1 is 25% lower than the exercise assigned value.)

Laboratory No. 14 15 16 17 18 19 20 21 22 23 24 25 26

naphthalene 0.2 -0.2 -0.3 -0.4 -0.2 -0.4 0.1 -0.9 -0.3 0.0 0.6 0.4 0.0

biphenyl 0.2 -0.2 -0.4 1.8 0.2 -0.6 0.2 -0.5 -0.7 -0.5 1.9 0.6 -0.4

acenaphthene 0.8 4.0 -1.0 4.1 0.0 0.1 1.4 -0.6 1.2 -2.0

acenaphthylene -1.0 -1.8 9.5 2.2 0.0 -1.3 -0.8

fluorene -0.8 0.3 -0.8 3.6 -0.7 0.0 0.4 -0.6 -0.2 0.7 -1.0 2.2 -0.6

phenanthrene 0.1 0.3 -0.7 -3.3 -1.0 -0.7 0.3 -0.5 0.1 0.0 0.8 -0.9 -0.1

anthracene 1.4 3.2 -1.0 -0.7 -0.1 -2.7 -2.0

fluoranthene -0.1 0.5 -0.4 5.3 1.5 -0.3 -1.1 -0.5 -2.2

pyrene 0.4 -0.5 0.4 -1.0 -1.1 1.4 -1.3 0.7 -0.7 -0.2 -0.3 0.4

benzo[b]fluorene -0.1 -0.3 -0.8 1.9

benz[a]anthracene 0.3 3.5 -0.4 0.1 1.0 -1.0 -1.6 -1.2 1.4

chrysene

triphenylene

benzo[b ]fluoranthene -0.2 -0.2 -1.1 1.3 0.3 -0.6 -0.8 0.0 -0.8

benzo[j ]fluoranthene

benzo[k ]fluoranthene

benzo[a]fluoranthene

benzo[e]pyrene -0.1 1.7 -0.4 0.1 0.7 -0.1 -0.5 -0.4 0.0 -0.3

benzo[a]pyrene 1.5 0.1 0.6 -1.1

perylene 0.9 -0.7 -1.4 -2.4

indeno[1,2,3-cd]pyrene

benzo[ghi]perylene -0.5 -0.2 -0.2 -0.8 0.0 0.2

dibenz[a,h]anthracene

cis/trans-decalin -1.1 -1.0 -0.7 2.7

dibenzofuran -1.0 -1.3 0.4 -0.2 0.2 -0.2

retene -2.2 0.9 1.9

benzothiophene -1.0 -1.9 2.8 2.4

dibenzothiophene -0.4 0.4 -0.6 -1.2 0.4 0.9 -0.5 0.0 -0.2 -0.1 4.7 0.0

naphthobenzothiophene 1.4 -3.0 3.0 1.5

chrysene + triphenylene -0.2 -0.5 0.2 0.5
24

Table 5. Crude Oil (QA10OIL01): z scores (25% by laboratory) - Alkylated PAHs 

(z=+1 is 25% higher than the exercise assigned value; z=-1 is 25% lower than the exercise assigned value.)

Laboratory No. 1 2 3 4 5 6 7 8 9 10 11 12 13

1-methylnaphthalene 0.1 -0.3 -0.4 0.0 1.1 -1.2 0.0 -0.1 -0.3 0.0 0.3 -0.2

2-methylnaphthalene 0.0 0.6 0.1 0.5 0.0 1.2 -1.3 0.4 0.7 -0.4 -0.5 0.5 0.2

2,6-dimethylnaphthalene 0.9 0.4 0.5 1.7 -2.2 0.5 0.5 0.2 1.1 -3.0

1,6,7-trimethylnaphthalene -0.9 1.9 -2.6 -0.2 -1.3 -1.8 -0.2

1-methylphenanthrene -0.1 0.8 -1.7 1.3 0.3 0.3 0.7 -1.5 1.4 -0.9

C1-decalins 0.2 -0.7 -0.6

C2-decalins 1.7 -1.3 -1.0

C3-decalins 1.9 -1.5 -1.9

C4-decalins 0.6 -1.2 -0.8

C1-naphthalenes 1.2 -1.3 -0.7 -0.5 -0.8 1.6 -1.0 -0.6 1.3

C2-naphthalenes -0.3 -0.5 -0.8 -0.1 -1.7 -0.6 2.1 -0.7 2.9 -0.3 1.2

C3-naphthalenes -0.2 -1.0 -1.1 0.4 -2.0 -0.5 3.6 -0.6 2.3 0.0 1.6

C4-naphthalenes 0.4 -1.1 -1.3 0.7 -1.7 0.1 3.6 -0.1 2.7 0.3 -2.2

C1-benzothiophenes 1.4 -1.2 -0.6 17.2

C2-benzothiophenes -0.2 -1.1 0.4 485.9

C3-benzothiophenes

C4-benzothiophenes 0.4 -0.5 0.3

C1-fluorenes -0.2 -1.2 -0.4 0.5 -1.9 -0.4 -0.1 -0.4 -1.0 -0.1 4.5

C2-fluorenes 0.2 -0.4 -0.8 1.3 -2.1 0.1 0.2 0.3 -0.4 0.6

C3-fluorenes -0.2 -0.5 -0.3 1.9 -2.2 0.1 0.8 0.8 -0.2 0.7 3.8

C1-phenanthrenes/anthracenes 1.3 -0.6 -0.5 -0.2 -0.6 -0.7 1.1 -1.2 0.2 0.1 1.0

C2-phenanthrenes/anthracenes 0.7 -0.4 -0.2 0.8 -0.6 -0.3 1.7 -0.6 0.9 0.8 1.0

C3-phenanthrenes/anthracenes 0.7 -1.0 -0.5 0.8 -0.2 0.0 2.5 -0.4 1.3 0.7 -2.1

C4-phenanthrenes/anthracenes 1.9 -0.5 -0.2 0.0 -1.2 0.7 2.7 0.2 12.6 0.1 -2.2

C1-dibenzothiophenes 0.3 0.8 0.2 -0.9 0.6 -1.3 1.2 0.2 0.3 1.7 -3.8

C2-dibenzothiophenes -1.0 0.8 -0.2 -0.4 0.7 -1.1 2.1 1.0 0.7 2.4 -1.3

C3-dibenzothiophenes -0.5 0.8 -0.1 0.2 0.1 -0.9 1.5 1.0 2.2 -1.0

C4-dibenzothiophenes -0.7 -0.1 -0.2 1.0 -0.8 1.8 0.6 1.8 -0.8

C1-fluoranthenes/pyrenes -0.7 -0.4 -0.3 1.4 -0.4 0.7 0.6 0.8 -0.3 0.9 -1.1

C2-fluoranthenes/pyrenes -0.2 -0.4 -1.2 1.1 -1.0 0.2 0.2 0.3 -0.4 0.5

C3-fluoranthenes/pyrenes 0.7 -0.7 -1.6 1.0 -1.4 0.3 1.0 0.3 -0.1 1.2 -1.9

C4-fluoranthenes/pyrenes 0.3 -1.5 0.6 0.6 -0.2 -3.7

C1-naphthobenzothiophenes 0.0 1.2 2.0 -1.8

C2-naphthobenzothiophenes -0.7 0.8 2.0 -1.8

C3-naphthobenzothiophenes -0.5 1.1 1.0 -1.9

C4-naphthobenzothiophenes -1.1 0.4 -0.1 -2.4

C1-chrysenes 0.0 -0.8 0.0 0.2 -0.4 0.0 0.4 -0.5 1.0 2.6

C2-chrysenes -0.1 -1.2 -0.1 -0.2 -0.5 -0.4 0.4 -0.5 0.8 -3.5

C3-chrysenes -0.3 -1.2 0.2 0.5 -1.6 -0.3 0.3 0.0 1.0

C4-chrysenes 0.3 -1.6 -1.6 0.4 0.4 -1.0 0.6 -3.8
25

Table 5 (cont). Crude Oil (QA10OIL01): z scores (25% by laboratory) - Alkylated PAHs 

(z=+1 is 25% higher than the exercise assigned value; z=-1 is 25% lower than the exercise assigned value.)

Laboratory No. 14 15 16 17 18 19 20 21 22 23 24 25 26

1-methylnaphthalene 0.3 0.1 -0.4 3.7 -0.1 -0.3 0.3 -0.4 0.2 0.1 1.3 0.0

2-methylnaphthalene -0.3 0.7 -0.2 -1.3 -0.2 -0.1 0.8 -0.1 0.2 0.0 -1.2 -0.1

2,6-dimethylnaphthalene 1.1 0.3 -2.8 0.2 0.4 1.7 -0.6 0.2 0.3 -0.6 -1.3 0.4

1,6,7-trimethylnaphthalene 0.2 2.1 0.9 -0.2 0.6 -0.4 -1.6 2.8 0.7

1-methylphenanthrene 1.0 0.2 -0.7 0.7 -1.8 0.1 2.1 -0.2 -1.2 0.3 0.2 -0.1 -1.5

C1-decalins -0.7 -0.7 2.5

C2-decalins -0.1 -1.3 1.9

C3-decalins 0.8 -2.2 2.9

C4-decalins 0.6 -1.9 2.7

C1-naphthalenes -1.1 -0.5 -0.9 0.7 -0.8 0.9 1.9 0.9 -0.5 1.2 0.1 -1.0

C2-naphthalenes -1.0 -0.4 -0.6 2.3 -0.3 2.1 -0.2 -2.3 -1.0 1.8 -0.8 -0.9

C3-naphthalenes -1.0 -0.4 -0.7 16.9 -0.7 2.5 -0.3 -2.4 -1.1 2.3 0.3 -0.9

C4-naphthalenes -1.0 -0.2 -1.0 0.2 0.0 -0.8 -0.1 -2.1 -1.0 0.6 5.0 -0.9

C1-benzothiophenes 3.5 -0.8 -1.1 -1.1

C2-benzothiophenes 3.1 -0.9 -1.1 -0.2

C3-benzothiophenes

C4-benzothiophenes 0.2 0.4 -0.7

C1-fluorenes -1.1 -0.6 -1.1 5.2 -1.0 2.7 -0.3 -0.9 -0.7 -0.1 -0.1 -1.4

C2-fluorenes -0.6 -0.2 -0.9 -3.4 -0.3 1.2 1.0 -0.3 -0.4 0.2 0.4 -1.1

C3-fluorenes -0.4 -0.3 -0.9 -2.2 0.0 -1.6 1.2 -0.1 -0.4 -1.4 0.0 -0.9

C1-phenanthrenes/anthracenes -1.0 -0.3 -1.2 2.4 -1.4 2.9 -0.1 -0.6 -0.6 0.8 0.1 -0.8

C2-phenanthrenes/anthracenes -0.6 -0.2 -1.0 54.0 -1.0 -1.1 0.4 -1.5 -0.5 1.5 0.3 -0.3

C3-phenanthrenes/anthracenes -0.4 0.2 6.4 -1.0 -0.1 0.6 -1.3 -0.9 0.2 1.5 -0.6

C4-phenanthrenes/anthracenes 0.2 0.3 -3.0 0.2 -2.9 1.2 -0.6 -1.1 -1.1 2.6 -0.4

C1-dibenzothiophenes -1.2 -0.9 -1.7 3.0 -0.7 -1.6 -0.8 0.9 0.4 0.2 -1.0 0.4

C2-dibenzothiophenes -1.0 -0.4 -2.1 -3.7 0.0 -1.7 -0.5 1.2 0.2 0.4 -0.9 1.1

C3-dibenzothiophenes -1.1 -0.5 -0.3 0.1 -0.1 -1.1 0.2 -0.5 -0.9 0.8

C4-dibenzothiophenes -1.1 0.0 -2.5 0.2 3.4 0.2 -2.1 -0.8 0.0

C1-fluoranthenes/pyrenes -0.2 -1.0 14.9 -1.1 6.7 1.3 1.4 0.5 -0.6 -1.2 -0.3

C2-fluoranthenes/pyrenes -0.2 -0.3 -0.9 2.0 2.5 0.2 -0.5 -1.3 -0.6

C3-fluoranthenes/pyrenes -0.4 -0.5 7.7 -0.2 1.3 1.8 0.9 -0.8 -0.8 -0.2

C4-fluoranthenes/pyrenes -0.9 -0.5 3.4 1.1 -1.7 -0.5 -0.7

C1-naphthobenzothiophenes -3.1 -0.5 -1.8 0.6 0.4

C2-naphthobenzothiophenes -0.1 -1.6 1.1 0.2

C3-naphthobenzothiophenes -1.4 0.2 1.4 0.0

C4-naphthobenzothiophenes 0.2 2.0 0.9

C1-chrysenes -0.5 0.1 -1.3 5.0 -0.4 0.9 0.8 0.8 0.3 -0.1 0.2 -0.5

C2-chrysenes -0.6 0.1 7.2 0.0 0.6 1.0 1.0 0.4 -0.5 0.3 -0.5

C3-chrysenes -0.3 -0.7 0.1 1.6 1.2 -3.5 2.1 -2.3 0.2 -0.5

C4-chrysenes -1.0 0.7 1.4 0.9 -3.5 0.3 0.3
26

Table 6. Crude Oil (QA10OIL01): z scores (25% by laboratory) - Biomarkers

(z=+1 is 25% higher than the exercise assigned value; z=-1 is 25% lower than the exercise assigned value.)

Laboratory No. 1 2 3 4 5 6 7 8 9 10 11 12 13

Carbazole -0.8 0.3

18a(H)-22,29,30-Trisnorhopane -1.0 -0.4 -1.0 0.6

17a(H)-22,29,30-Trisnorhopane -0.1 -1.2 2.7 -1.2 -0.8 11.2

17α(H),21β(H)-30-Norhopane -0.8 -1.0 -0.3 -1.0

18a(H)-30-Norneohopane -1.2 2.5 -1.1 6.5

17a(H)-Diahopane -0.9 0.7 0.1 1.1

17α(H),21β(H)-Hopane 0.3 -1.1 0.5 0.9 -1.4 -0.5 0.1

17α(H),21β(H)-22R-Homohopane -0.4 -1.3 1.3 -0.1 -0.8

17α(H),21β(H)-22S-Homohopane -0.1 -0.4 0.4 -1.7 0.0

13b(H)17a(H)-Diacholestane 20S 0.7 1.0 -0.6 0.2

5a(H),14a(H),17a(H)-Cholestane 20S 0.9 -0.3 0.5

5a(H),14a(H),17a(H)-Cholestane 20R -1.8 1.6 2.8 -2.1 0.9 -0.9

5a(H),14a(H),17a(H)-24-Ethylcholestane 20S 0.2 0.3 -1.0 -1.0

5a(H),14a(H),17a(H)-24-Ethylcholestane 20R -0.7 -0.2 1.8 3.0 -0.9 -1.6

5a(H),14b(H),17b(H)-Cholestane 20R -0.1 -0.1 1.1 -1.6 0.0

5a(H),14b(H),17b(H)-Cholestane 20S -0.1 1.9 -1.4 0.0

5a(H),14b(H),17b(H)-24-Ethylcholestane 20R -0.1 -0.3 1.2 -1.1 0.7

5a(H),14b(H),17b(H)-24-Ethylcholestane 20S -0.3 1.2 -1.6 -0.4
27

Table 6 (cont). Crude Oil (QA10OIL01): z scores (25% by laboratory) - Biomarkers

(z=+1 is 25% higher than the exercise assigned value; z=-1 is 25% lower than the exercise assigned value.)

Laboratory No. 14 15 16 17 18 19 20 21 22 23 24 25 26

Carbazole 1.6 -1.1

18a(H)-22,29,30-Trisnorhopane 0.1 0.9 0.9 -0.1

17a(H)-22,29,30-Trisnorhopane -1.1 1.1 8.5 0.0 0.2 0.5

17α(H),21β(H)-30-Norhopane -0.4 -3.6 11.0 2.0 0.3 0.2 1.0

18a(H)-30-Norneohopane -0.9 -0.5 0.6 0.7

17a(H)-Diahopane 0.3 -2.4 1.1

17α(H),21β(H)-Hopane -0.6 -0.1 1.4 1.3 0.2 -0.1 -0.9

17α(H),21β(H)-22R-Homohopane -0.7 -0.5 0.5 0.7 -0.2 1.5

17α(H),21β(H)-22S-Homohopane -0.1 -0.8 0.8 0.6 -0.2 1.6

13b(H)17a(H)-Diacholestane 20S -0.1 -0.8 3.5 -0.4

5a(H),14a(H),17a(H)-Cholestane 20S 0.1 -2.3 4.2 0.0 -3.0

5a(H),14a(H),17a(H)-Cholestane 20R 1.0 -2.2 1.4 3.8 0.6 -2.1 -3.0

5a(H),14a(H),17a(H)-24-Ethylcholestane 20S 0.6 3.5 -0.2 -2.4

5a(H),14a(H),17a(H)-24-Ethylcholestane 20R -0.6 2.0 -0.9 -1.6 -1.9

5a(H),14b(H),17b(H)-Cholestane 20R -1.3 0.4 3.1 -0.5 -0.3 -0.6

5a(H),14b(H),17b(H)-Cholestane 20S -1.0 -1.0 3.2 -0.4 -1.1

5a(H),14b(H),17b(H)-24-Ethylcholestane 20R -0.2 -1.4 -1.1 3.7 0.7 -0.2 -1.9

5a(H),14b(H),17b(H)-24-Ethylcholestane 20S -0.9 0.9 2.6 -0.8 -0.8
28

Table 7. Crude Oil (QA10OIL01): Laboratory relative standard deviatins of three replicates - PAHs

Laboratory No. 1 2 3 4 5 6 7 8 9 10 11 12 13

naphthalene 1.9% 38.3% 2.9% 1.7% 4.4% 2.1% 7.3% 5.9% 6.0% 5.1% 8.3% 17.0% 0.5%

biphenyl 2.3% 2.8% 2.0% 7.1% 7.8% 5.3% 2.3% 6.7% 3.5% 4.8% 3.9% 7.7%

acenaphthene 7.3% 13.9% 2.8% 6.7% 6.1% 3.3% 1.1% 4.4% 2.9%

acenaphthylene 13.1% 5.1% 3.1% 12.7% 6.1% 5.1% 6.1% 25.4%

fluorene 2.5% 33.4% 4.0% 3.7% 4.0% 4.9% 6.7% 3.8% 6.7% 5.8% 5.5% 3.4% 1.8%

phenanthrene 3.4% 33.7% 2.9% 0.6% 2.1% 4.8% 9.6% 5.1% 6.0% 2.4% 4.9% 1.7% 2.1%

anthracene 5.7% 5.8% 1.8% 18.4% 19.1% 9.8% 5.4% 7.3% 28.1%

fluoranthene 6.3% 2.8% 11.1% 9.0% 8.6% 8.3% 1.7% 27.2% 17.8%

pyrene 3.0% 8.8% 3.1% 4.6% 8.0% 8.3% 7.0% 7.8% 1.1% 5.6% 4.4%

benzo[b]fluorene 3.7% 5.9% 26.4% 36.7%

benz[a]anthracene 2.3% 3.3% 2.9% 5.4% 2.3% 10.6% 9.1% 3.1% 11.0% 8.7%

chrysene 3.8% 3.7% 5.9% 19.2% 1.7% 9.4% 3.7% 6.8% 3.5%

triphenylene 3.7% 4.1%

benzo[b ]fluoranthene 3.4% 10.8% 7.2% 25.4% 2.7% 6.2% 12.8% 6.4% 3.0% 6.3%

benzo[j ]fluoranthene 5.5% 57.3%

benzo[k ]fluoranthene 5.9% 9.7%

benzo[a]fluoranthene

benzo[e]pyrene 2.6% 2.4% 5.4% 7.7% 5.0% 6.2% 12.2% 5.9% 5.9% 5.8% 83.8%

benzo[a]pyrene 2.7% 7.0% 6.3% 6.8% 6.7% 2.6%

perylene 3.1% 3.5% 9.3% 4.6% 2.8% 93.0%

indeno[1,2,3-cd]pyrene 6.5% 0.3%

benzo[ghi]perylene 5.5% 7.7% 8.3% 3.7% 14.3% 36.6%

dibenz[a,h]anthracene 6.2% 6.4% 2.9% 8.2% 2.7% 13.9%

cis/trans-decalin 3.2% 0.7% 1.8% 3.1% 1.9%

dibenzofuran 2.1% 2.5% 6.2% 6.9% 2.7% 3.3% 2.0%

retene 3.0% 10.4% 3.3% 18.9% 4.8%

benzothiophene 3.1% 8.2% 12.3% 5.2%

dibenzothiophene 6.2% 2.9% 1.4% 8.5% 2.9% 8.5% 2.9% 5.9% 13.1% 1.5% 2.8% 3.4%

naphthobenzothiophene 3.9% 3.9% 11.4% 10.2% 12.9%
29

Table 7 (cont). Crude Oil (QA10OIL01): Laboratory relative standard deviatins of three replicates - PAHs 

Laboratory No. 14 15 16 17 18 19 20 21 22 23 24 25 26

naphthalene 2.6% 1.9% 6.4% 4.5% 1.0% 12.5% 1.0% 4.1% 3.3% 0.3% 2.8% 2.0% 4.9%

biphenyl 3.9% 39.6% 8.4% 3.9% 12.3% 7.0% 5.6% 0.7% 4.4% 2.0% 2.6% 5.1% 3.3%

acenaphthene 2.8% 7.9% 2.5% 11.0% 33.4% 6.5% 9.2% 1.1% 10.1% 23.7%

acenaphthylene 3.3% 71.3% 7.0% 7.3% 7.0% 7.2% 5.9%

fluorene 3.1% 2.8% 4.9% 4.3% 1.3% 5.7% 8.5% 7.3% 5.1% 0.9% 2.2% 7.5% 0.0%

phenanthrene 0.7% 3.3% 7.2% 20.3% 0.3% 7.7% 1.8% 1.1% 4.2% 1.0% 19.6% 2.0% 4.2%

anthracene 2.9% 5.8% 2.7% 1.6% 5.8% 3.9% 1.3%

fluoranthene 2.8% 3.6% 2.6% 8.9% 10.8% 5.2% 4.1% 8.6% 5.4%

pyrene 5.5% 6.7% 6.2% 3.4% 5.3% 12.7% 4.4% 3.3% 8.6% 2.9% 2.8% 5.9%

benzo[b]fluorene 2.6% 9.0% 4.8% 4.5%

benz[a]anthracene 10.7% 39.7% 2.6% 39.4% 1.6% 4.4% 10.2% 3.9% 5.1%

chrysene 4.2% 2.5% 8.7% 5.4% 0.6% 0.9% 6.0% 7.2% 8.0% 1.4% 2.0% 2.5%

triphenylene 1.4% 5.9%

benzo[b ]fluoranthene 3.0% 21.4% 2.0% 42.8% 3.5% 7.2% 2.7% 3.1% 1.2%

benzo[j]fluoranthene 20.7% 7.5%

benzo[k ]fluoranthene 18.4% 6.8% 9.2%

benzo[a]fluoranthene 5.4%

benzo[e]pyrene 2.8% 2.8% 1.7% 9.3% 11.5% 0.5% 2.6% 5.7% 3.0% 5.4%

benzo[a]pyrene 1.9% 4.4% 4.9% 2.7%

perylene 6.7% 32.4% 2.8% n=1

indeno[1,2,3-cd]pyrene 2.6%

benzo[ghi]perylene 0.8% 9.0% 4.9% 10.3% 4.7% 4.5%

dibenz[a,h]anthracene 3.7% 2.6% 7.4% 20.5% 4.2% 2.8%

cis/trans-decalin 2.1% 1.7% 4.5% 4.0%

dibenzofuran 2.5% 79.2% 4.6% 2.7% 3.1% 2.2%

retene 5.0% 5.2% 2.0%

benzothiophene 1.5% 9.0% 6.1% 2.7%

dibenzothiophene 2.9% 1.9% 15.0% 1.2% 8.9% 9.9% 1.3% 4.5% 1.9% 1.5% 1.9% 4.2%

naphthobenzothiophene 2.2% 11.6% 4.8% 5.9%
30

Table 8. Crude Oil (QA10OIL01): Laboratory relative standard deviatins of three replicates - Alkylated PAHs 

Laboratory No. 1 2 3 4 5 6 7 8 9 10 11 12 13

1-methylnaphthalene 8.6% 4.0% 1.5% 9.1% 10.6% 4.1% 6.6% 5.8% 4.8% 8.8% 5.2% 2.5%

2-methylnaphthalene 5.7% 39.9% 4.4% 1.1% 7.1% 8.6% 5.8% 6.7% 7.1% 5.0% 9.1% 7.7% 1.3%

2,6-dimethylnaphthalene 3.9% 3.0% 0.5% 8.7% 9.3% 6.4% 6.5% 1.1% 6.1% 5.7%

1,6,7-trimethylnaphthalene 2.5% 6.0% 5.3% 5.8% 5.0% 4.3% 18.9%

1-methylphenanthrene 8.9% 3.2% 1.4% 3.4% 6.7% 0.6% 6.5% 14.0% 4.0% 8.9%

C1-decalins 1.6% 3.2% 3.4%

C2-decalins 4.4% 8.3% 2.4%

C3-decalins 4.9% 10.8% 4.0%

C4-decalins 3.0% 16.8% 6.1%

C1-naphthalenes 0.6% 4.1% 1.7% 6.3% 2.4% 6.7% 5.7% 10.9% 1.8%

C2-naphthalenes 6.7% 4.2% 4.1% 6.5% 5.0% 3.0% 6.9% 7.4% 9.1% 6.4% 2.1%

C3-naphthalenes 5.5% 3.4% 0.9% 6.7% 5.7% 3.3% 7.1% 10.0% 10.5% 3.0% 11.8%

C4-naphthalenes 4.0% 3.4% 3.5% 7.4% 6.5% 3.7% 6.5% 9.3% 4.1% 8.0% 6.2%

C1-benzothiophenes 6.6% 2.1% 6.1% 12.5%

C2-benzothiophenes 3.2% 7.4% 3.6% 29.4%

C3-benzothiophenes 0.1% 6.3% 3.6% 4.5%

C4-benzothiophenes 6.6% 14.4% 1.7%

C1-fluorenes 4.8% 2.6% 4.8% 6.1% 5.7% 2.9% 7.1% 3.4% 3.8% 6.9% 6.0%

C2-fluorenes 3.5% 3.0% 1.8% 9.7% 3.5% 3.5% 6.7% 8.8% 2.1% 8.5%

C3-fluorenes 4.0% 4.7% 11.1% 9.2% 3.7% 3.3% 7.7% 7.8% 8.3% 11.0% 44.4%

C1-phenanthrenes/anthracenes 1.8% 3.3% 2.6% 3.8% 4.1% 2.6% 6.7% 8.2% 1.0% 2.4% 5.0%

C2-phenanthrenes/anthracenes 2.7% 2.9% 4.1% 4.4% 6.3% 2.6% 6.8% 9.2% 2.8% 2.9% 10.6%

C3-phenanthrenes/anthracenes 3.7% 2.6% 8.0% 4.6% 4.9% 1.9% 6.2% 11.1% 3.9% 3.0% 8.3%

C4-phenanthrenes/anthracenes 6.8% 5.1% 5.8% 7.8% 5.2% 3.5% 7.3% 15.6% 2.4% 2.3% 16.1%

C1-dibenzothiophenes 6.3% 3.0% 3.6% 4.7% 8.1% 2.8% 6.7% 12.7% 0.4% 6.5% 3.5%

C2-dibenzothiophenes 11.3% 4.0% 4.1% 4.0% 6.0% 3.0% 8.3% 18.4% 0.3% 2.0% 8.1%

C3-dibenzothiophenes 3.2% 3.6% 6.5% 9.4% 2.5% 2.2% 7.0% 14.7% 6.1% 8.9%

C4-dibenzothiophenes 6.1% 2.4% 5.4% 2.9% 2.5% 6.4% 10.7% 7.4% 19.7%

C1-fluoranthenes/pyrenes 9.7% 2.9% 2.5% 10.6% 8.1% 1.0% 6.8% 15.9% 7.3% 2.4% 9.4%

C2-fluoranthenes/pyrenes 9.4% 6.6% 2.3% 6.9% 8.4% 1.5% 6.3% 15.3% 5.2% 5.6%

C3-fluoranthenes/pyrenes 2.4% 4.0% 7.7% 7.4% 8.4% 3.0% 6.2% 21.1% 6.1% 4.6% 5.9%

C4-fluoranthenes/pyrenes 6.3% 6.9% 8.4% 12.3% 5.8% 17.3%

C1-naphthobenzothiophenes 5.2% 6.9% 2.0% 8.9%

C2-naphthobenzothiophenes 0.9% 7.5% 4.0% 8.2%

C3-naphthobenzothiophenes 5.6% 7.8% 3.9% 11.7%

C4-naphthobenzothiophenes 4.5% 8.0% 19.1% 15.7%

C1-chrysenes 4.1% 3.6% 2.1% 5.1% 8.1% 3.2% 6.6% 7.2% 8.0% 4.5%

C2-chrysenes 5.3% 1.5% 3.1% 4.7% 9.2% 5.5% 5.6% 9.6% 8.1% 19.6%

C3-chrysenes 5.5% 10.4% 1.4% 6.3% 7.1% 8.5% 6.3% 9.4% 9.4%

C4-chrysenes 6.2% 4.6% 6.6% 2.1% 5.5% 19.6% 8.6% 18.4%
31

Table 8 (cont). Crude Oil (QA10OIL01): Laboratory relative standard deviatins of three replicates - Alkylated PAHs 

Laboratory No. 14 15 16 17 18 19 20 21 22 23 24 25 26

1-methylnaphthalene 2.0% 1.3% 5.7% 24.3% 0.5% 9.7% 9.8% 5.1% 2.8% 0.7% 4.0% 9.1%

2-methylnaphthalene 2.7% 1.3% 6.0% 1.6% 0.8% 10.7% 10.8% 4.1% 3.3% 0.4% 1.4% 3.7%

2,6-dimethylnaphthalene 2.3% 1.2% 3.1% 0.4% 10.1% 7.7% 3.5% 4.4% 0.7% 1.5% 5.8% 0.0%

1,6,7-trimethylnaphthalene 2.4% 1.2% 0.5% 5.8% 13.2% 5.0% 3.0% 10.7% 14.6%

1-methylphenanthrene 7.3% 6.7% 6.1% 2.8% 0.6% 9.3% 2.8% 6.0% 3.7% 1.6% 3.7% 3.1% 5.6%

C1-decalins 5.6% 7.1% 0.0%

C2-decalins 0.0% 6.7% 5.6%

C3-decalins 0.0% 8.4% 4.0%

C4-decalins 1.4% 6.9% 4.9%

C1-naphthalenes 3.7% 1.1% 7.0% 3.7% 0.0% 10.8% 10.5% 4.5% 3.1% 0.4% 1.2% 6.3%

C2-naphthalenes 5.2% 1.6% 9.9% 6.5% 0.0% 11.3% 7.2% 3.5% 4.2% 0.8% 7.5% 3.1%

C3-naphthalenes 2.1% 1.5% 8.5% 3.0% 0.0% 11.2% 17.0% 5.7% 4.5% 0.4% 3.3% 4.7%

C4-naphthalenes 1.7% 0.7% 8.4% 38.5% 1.4% 13.8% 17.2% 6.6% 4.8% 0.8% 13.6% 14.2%

C1-benzothiophenes 17.7% 1.8% 4.1% 2.0%

C2-benzothiophenes 26.2% 5.2% 3.9% 2.8%

C3-benzothiophenes 11.8% 1.9% 4.0% 4.3%

C4-benzothiophenes 3.3% 5.3% 6.5%

C1-fluorenes 1.9% 1.0% 7.3% 10.4% 2.3% 7.6% 12.7% 2.0% 4.6% 0.5% 3.0% 2.7%

C2-fluorenes 3.0% 1.6% 15.2% 27.1% 0.0% 15.2% 5.9% 8.3% 3.9% 1.6% 8.9% 2.1%

C3-fluorenes 4.6% 4.0% 12.2% 12.0% 2.0% 10.5% 8.8% 2.7% 5.1% 2.9% 15.0% 4.5%

C1-phenanthrenes/anthracenes 3.1% 2.6% 6.6% 5.2% 0.0% 11.5% 2.2% 1.7% 4.6% 1.2% 2.9% 4.3%

C2-phenanthrenes/anthracenes 2.1% 2.3% 6.5% 6.4% 1.2% 9.8% 7.2% 1.2% 5.0% 2.2% 2.6% 3.7%

C3-phenanthrenes/anthracenes 4.5% 2.8% 3.5% 1.9% 10.0% 16.3% 3.3% 5.8% 3.2% 5.9% 3.3%

C4-phenanthrenes/anthracenes 2.5% 5.0% 27.9% 2.8% 9.0% 10.3% 1.9% 6.8% 4.7% 6.2% 3.3%

C1-dibenzothiophenes 4.5% 10.0% 9.2% 3.5% 5.9% 13.1% 1.8% 0.4% 5.5% 0.7% 7.9% 4.2%

C2-dibenzothiophenes 2.2% 0.4% 8.1% 14.9% 0.0% 10.2% 3.6% 0.7% 4.0% 1.2% 7.8% 10.2%

C3-dibenzothiophenes 3.3% 5.0% 20.2% 0.0% 13.2% 5.5% 6.2% 1.8% 11.8% 12.4%

C4-dibenzothiophenes 8.2% 5.0% 31.3% 1.9% 24.0% 6.3% 4.1% 10.3% 7.1%

C1-fluoranthenes/pyrenes 3.6% 2.5% 39.7% 2.9% 19.4% 1.2% 9.7% 5.4% 3.5% 8.1% 8.3%

C2-fluoranthenes/pyrenes 2.6% 2.2% 0.6% 12.4% 15.3% 5.6% 5.7% 9.1% 9.1%

C3-fluoranthenes/pyrenes 2.5% 6.5% 6.1% 0.0% 17.5% 4.2% 7.7% 5.2% 10.1% 8.3%

C4-fluoranthenes/pyrenes 53.6% 1.4% 12.2% 6.2% 7.1% 10.9% 7.5%

C1-naphthobenzothiophenes 17.2% 1.2% 6.7% 6.5% 3.9%

C2-naphthobenzothiophenes 0.9% 12.9% 6.3% 3.7%

C3-naphthobenzothiophenes 6.3% 2.0% 7.0% 3.3%

C4-naphthobenzothiophenes 3.8% 6.3% 13.2%

C1-chrysenes 2.7% 1.4% 3.8% 2.4% 2.2% 11.5% 6.7% 12.5% 4.6% 2.3% 0.5% 1.6%

C2-chrysenes 3.5% 5.1% 20.6% 0.0% 13.4% 14.3% 5.0% 4.2% 4.1% 5.4% 5.1%

C3-chrysenes 3.2% 22.7% 0.6% 11.1% 18.2% 8.5% 3.1% 11.2% 7.8% 2.2%

C4-chrysenes 2.9% 3.4% 14.5% 4.0% 24.0% 1.6% 4.1%
32

Table 9. Crude Oil (QA10OIL01): Laboratory relative standard deviatins of three replicates - Biomarkers

Laboratory No. 1 2 3 4 5 6 7 8 9 10 11 12 13

Carbazole 17.3% 13.6% 7.2%

18a(H)-22,29,30-Trisnorneohopane 6.2% 16.6% 4.0% 1.4%

17a(H)-22,29,30-Trisnorhopane 3.5% 6.2% 39.9% 6.4% 2.9% 6.1%

17α(H),21β(H)-30-Norhopane 9.3% 7.8% 71.5% 6.7%

18a(H)-30-Norneohopane 4.9% 79.7% 4.8% 6.8%

17a(H)-Diahopane 3.4% 39.1% 18.8% 15.8%

17α(H),21β(H)-Hopane 5.0% 7.6% 6.7% 12.1% 8.9% 3.1% 8.2%

17α(H),21β(H)-22R-Homohopane 7.8% 9.1% 30.6% 11.2% 5.0%

17α(H),21β(H)-22S-Homohopane 3.5% 5.8% 40.9% 7.7% 3.9%

13b(H)17a(H)-Diacholestane 20S 3.2% 5.5% 6.5% 3.7%

5a(H),14a(H),17a(H)-Cholestane 20S 7.6% 12.0% 1.6%

5a(H),14a(H),17a(H)-Cholestane 20R 3.3% 26.4% 16.7% 4.5% 2.0% 30.4%

5a(H),14a(H),17a(H)-24-Ethylcholestane 20S 4.1% 50.4% 6.2% 6.6%

5a(H),14a(H),17a(H)-24-Ethylcholestane 20R 4.4% 5.9% 19.0% 13.7% 2.8% 9.7%

5a(H),14b(H),17b(H)-Cholestane 20R 4.5% 5.9% 9.4% 6.3% 3.4%

5a(H),14b(H),17b(H)-Cholestane 20S 5.7% 20.8% 13.8% 3.5%

5a(H),14b(H),17b(H)-24-Ethylcholestane 20R 4.1% 8.1% 31.0% 5.6% 4.9%

5a(H),14b(H),17b(H)-24-Ethylcholestane 20S 24.8% 20.1% 12.1%
33

Table 9 (cont). Crude Oil (QA10OIL01): Laboratory relative standard deviatins of three replicates - Biomarkers

Laboratory No. 14 15 16 17 18 19 20 21 22 23 24 25 26

Carbazole 11.0% 6.3%

18a(H)-22,29,30-Trisnorneohopane 1.5% 26.5% 5.3% 7.4%

17a(H)-22,29,30-Trisnorhopane 5.1% 34.1% 9.1% 5.8% 4.8% 5.1%

17α(H),21β(H)-30-Norhopane 5.6% 20.4% 11.4% 19.2% 5.7% 5.2% 10.3%

18a(H)-30-Norneohopane 4.4% 22.7% 7.5% 4.1%

17a(H)-Diahopane 2.7% 23.0% 9.5%

17α(H),21β(H)-Hopane 3.4% 9.2% 3.1% 21.4% 8.3% 5.3% 7.3%

17α(H),21β(H)-22R-Homohopane 4.1% 0.5% 20.3% 4.4% 4.6% 9.2%

17α(H),21β(H)-22S-Homohopane 3.7% 19.1% 23.1% 7.2% 5.4% 10.1%

13b(H)17a(H)-Diacholestane 20S 1.1% 4.2% 22.9% 6.6%

5a(H),14a(H),17a(H)-Cholestane 20S 4.4% 4.7% 24.0% 5.7% 6.0%

5a(H),14a(H),17a(H)-Cholestane 20R 2.9% 12.5% 8.5% 21.1% 6.4% 6.4% 3.8%

5a(H),14a(H),17a(H)-24-Ethylcholestane 20S 7.4% 11.7% 1.4% 5.9%

5a(H),14a(H),17a(H)-24-Ethylcholestane 20R 4.0% 18.3% 12.6% 6.3% 3.0%

5a(H),14b(H),17b(H)-Cholestane 20R 5.2% 14.7% 27.1% 7.8% 6.7% 6.4%

5a(H),14b(H),17b(H)-Cholestane 20S 9.3% 25.5% 30.3% 7.6% 5.8%

5a(H),14b(H),17b(H)-24-Ethylcholestane 20R 3.8% 7.1% 11.7% 11.0% 7.7% 4.4% 4.6%

5a(H),14b(H),17b(H)-24-Ethylcholestane 20S 9.6% n=1 19.9% 18.5% 7.7%
34

Table 10. Crude Oil (QA10OIL01): p-scores (15%) - PAHs 

(p=1 reported rsd is 15%; p=2 reported rsd is 30%)

Laboratory No. 1 2 3 4 5 6 7 8 9 10 11 12 13

naphthalene 0.12 2.56 0.20 0.12 0.29 0.14 0.49 0.39 0.40 0.34 0.56 1.14 0.04

biphenyl 0.16 0.19 0.14 0.47 0.52 0.35 0.15 0.45 0.23 0.32 0.26 0.52

acenaphthene 0.48 0.92 0.19 0.44 0.41 0.22 0.08 0.29 0.19

acenaphthylene 0.88 0.34 0.21 0.85 0.41 0.34 0.41 1.69

fluorene 0.17 2.22 0.27 0.25 0.27 0.33 0.45 0.26 0.45 0.39 0.36 0.23 0.12

phenanthrene 0.23 2.25 0.19 0.04 0.14 0.32 0.64 0.34 0.40 0.16 0.32 0.11 0.14

anthracene 0.38 0.38 0.12 1.23 1.27 0.65 0.36 0.49 1.88

fluoranthene 0.42 0.19 0.00 0.74 0.60 0.57 0.55 0.11 1.81 1.18

pyrene 0.20 0.58 0.21 0.30 0.53 0.55 0.47 0.52 0.07 0.38 0.30

benzo[b]fluorene 0.24 0.39 1.76 2.44

benz[a]anthracene 0.15 0.22 0.19 0.36 0.16 0.70 0.61 0.21 0.74 0.58

chrysene 0.26 0.25 0.39 1.28 0.11 0.63 0.24 0.45 0.23

triphenylene 0.25 0.27

benzo[b ]fluoranthene 0.22 0.72 0.48 1.69 0.18 0.41 0.85 0.43 0.20 0.42

benzo[j]fluoranthene 0.37 3.82

benzo[k ]fluoranthene 0.40 0.64

benzo[a]fluoranthene

benzo[e]pyrene 0.17 0.16 0.36 0.51 0.34 0.41 0.81 0.39 0.39 0.38 5.59

benzo[a]pyrene 0.18 0.46 0.42 0.45 0.45 0.17

perylene 0.21 0.23 0.62 0.30 0.19 6.20

indeno[1,2,3-cd]pyrene 0.44 0.02

benzo[ghi]perylene 0.37 0.51 0.56 0.25 0.95 2.44

dibenz[a,h]anthracene 0.42 0.42 0.20 0.55 0.18 0.93

cis/trans-decalin 0.21 0.05 0.12 0.20 0.13

dibenzofuran 0.14 0.16 0.41 0.46 0.18 0.22 0.13

retene 0.20 0.69 0.22 1.26 0.32

benzothiophene 0.21 0.55 0.00 0.82 0.34

dibenzothiophene 0.41 0.20 0.09 0.56 0.20 0.57 0.19 0.39 0.87 0.10 0.18 0.23

naphthobenzothiophene 0.26 0.26 0.76 0.68 0.86
35

Table 10 (cont). Crude Oil (QA10OIL01): p-scores (15%) - PAHs 

(p=1 reported rsd is 15%; p=2 reported rsd is 30%)

Laboratory No. 14 15 16 17 18 19 20 21 22 23 24 25 26

naphthalene 0.17 0.13 0.43 0.30 0.07 0.83 0.07 0.27 0.22 0.02 0.19 0.13 0.33

biphenyl 0.26 2.64 0.56 0.26 0.82 0.47 0.37 0.05 0.29 0.13 0.17 0.34 0.22

acenaphthene 0.19 0.53 0.17 0.73 2.23 0.43 0.61 0.08 0.67 1.58

acenaphthylene 0.22 4.75 0.46 0.48 0.47 0.00 0.48 0.39

fluorene 0.21 0.18 0.33 0.29 0.09 0.38 0.57 0.49 0.34 0.06 0.15 0.50

phenanthrene 0.05 0.22 0.48 1.35 0.02 0.52 0.12 0.07 0.28 0.06 1.31 0.13 0.28

anthracene 0.19 0.38 0.00 0.18 0.11 0.38 0.26 0.09

fluoranthene 0.19 0.24 0.17 0.59 0.72 0.00 0.35 0.28 0.57 0.36

pyrene 0.37 0.45 0.41 0.23 0.35 0.85 0.29 0.22 0.58 0.19 0.19 0.39

benzo[b]fluorene 0.18 0.60 0.32 0.30

benz[a]anthracene 0.71 0.00 2.65 0.17 2.63 0.11 0.29 0.68 0.26 0.34

chrysene 0.28 0.16 0.58 0.36 0.04 0.06 0.40 0.48 0.53 0.09 0.13 0.17

triphenylene 0.09 0.40

benzo[b ]fluoranthene 0.20 1.43 0.13 2.85 0.23 0.48 0.18 0.21 0.08

benzo[j]fluoranthene 1.38 0.50

benzo[k ]fluoranthene 1.23 0.45 0.61

benzo[a]fluoranthene 0.36

benzo[e]pyrene 0.19 0.19 0.11 0.62 0.76 0.03 0.17 0.38 0.20 0.36

benzo[a]pyrene 0.13 0.29 0.33 0.18

perylene 0.45 2.16 0.19 n=1

indeno[1,2,3-cd]pyrene 0.17

benzo[ghi]perylene 0.06 0.60 0.33 0.69 0.32 0.30

dibenz[a,h]anthracene 0.25 0.18 0.50 1.37 0.28 0.19

cis/trans-decalin 0.14 0.12 0.30 0.27

dibenzofuran 0.17 5.28 0.31 0.18 0.21 0.15

retene 0.33 0.35 0.14

benzothiophene 0.10 0.60 0.41 0.18

dibenzothiophene 0.20 0.13 1.00 0.08 0.59 0.66 0.09 0.30 0.13 0.10 0.13 0.28

naphthobenzothiophene 0.14 0.77 0.32 0.39
36

Table 11. Crude Oil (QA10OIL01): p-scores (15%) - Alkylated PAHs 

(p=1 reported rsd is 15%; p=2 reported rsd is 30%)

Laboratory No. 1 2 3 4 5 6 7 8 9 10 11 12 13

1-methylnaphthalene 0.57 0.27 0.10 0.61 0.71 0.27 0.44 0.39 0.32 0.59 0.35 0.17

2-methylnaphthalene 0.38 2.66 0.29 0.07 0.47 0.58 0.39 0.45 0.47 0.33 0.61 0.52 0.09

2,6-dimethylnaphthalene 0.26 0.20 0.04 0.58 0.62 0.43 0.43 0.07 0.41 0.38

1,6,7-trimethylnaphthalene 0.17 0.40 0.35 0.39 0.34 0.29 1.26

1-methylphenanthrene 0.60 0.22 0.09 0.23 0.45 0.04 0.43 0.93 0.27 0.60

C1-decalins 0.11 0.21 0.23

C2-decalins 0.29 0.55 0.16

C3-decalins 0.33 0.72 0.26

C4-decalins 0.20 1.12 0.41

C1-naphthalenes 0.04 0.27 0.11 0.42 0.16 0.44 0.38 0.72 0.12

C2-naphthalenes 0.45 0.28 0.27 0.43 0.33 0.20 0.46 0.49 0.61 0.43 0.14

C3-naphthalenes 0.37 0.23 0.06 0.44 0.38 0.22 0.48 0.67 0.70 0.20 0.79

C4-naphthalenes 0.27 0.22 0.23 0.50 0.43 0.25 0.44 0.62 0.27 0.53 0.41

C1-benzothiophenes 0.44 0.14 0.41 0.84

C2-benzothiophenes 0.22 0.49 0.24 1.96

C3-benzothiophenes 0.01 0.42 0.24 0.30

C4-benzothiophenes 0.44 0.96 0.12

C1-fluorenes 0.32 0.17 0.32 0.41 0.38 0.19 0.48 0.23 0.25 0.46 0.40

C2-fluorenes 0.23 0.20 0.12 0.65 0.23 0.23 0.45 0.59 0.14 0.57

C3-fluorenes 0.26 0.32 0.74 0.61 0.25 0.22 0.51 0.52 0.55 0.74 2.96

C1-phenanthrenes/anthracenes 0.12 0.22 0.17 0.25 0.27 0.17 0.45 0.55 0.07 0.16 0.34

C2-phenanthrenes/anthracenes 0.18 0.20 0.28 0.29 0.42 0.17 0.45 0.61 0.19 0.20 0.70

C3-phenanthrenes/anthracenes 0.25 0.18 0.53 0.31 0.33 0.13 0.41 0.74 0.26 0.20 0.55

C4-phenanthrenes/anthracenes 0.45 0.34 0.39 0.52 0.35 0.23 0.49 1.04 0.16 0.15 1.07

C1-dibenzothiophenes 0.42 0.20 0.24 0.32 0.54 0.19 0.45 0.85 0.03 0.43 0.23

C2-dibenzothiophenes 0.75 0.27 0.28 0.27 0.40 0.20 0.56 1.23 0.02 0.14 0.54

C3-dibenzothiophenes 0.22 0.24 0.44 0.62 0.17 0.15 0.47 0.98 0.41 0.59

C4-dibenzothiophenes 0.41 0.16 0.36 0.19 0.00 0.17 0.43 0.71 0.49 1.31

C1-fluoranthenes/pyrenes 0.64 0.19 0.17 0.71 0.54 0.07 0.45 1.06 0.48 0.16 0.63

C2-fluoranthenes/pyrenes 0.63 0.44 0.15 0.46 0.56 0.10 0.42 1.02 0.34 0.38

C3-fluoranthenes/pyrenes 0.16 0.27 0.51 0.49 0.56 0.20 0.41 1.40 0.40 0.31 0.39

C4-fluoranthenes/pyrenes 0.42 0.46 0.56 0.82 0.39 1.15

C1-naphthobenzothiophenes 0.35 0.46 0.14 0.00 0.59

C2-naphthobenzothiophenes 0.06 0.50 0.27 0.55

C3-naphthobenzothiophenes 0.38 0.52 0.26 0.78

C4-naphthobenzothiophenes 0.30 0.53 1.27 1.05

C1-chrysenes 0.27 0.24 0.14 0.34 0.54 0.21 0.44 0.48 0.54 0.30

C2-chrysenes 0.35 0.10 0.21 0.31 0.62 0.37 0.37 0.64 0.54 1.31

C3-chrysenes 0.37 0.69 0.10 0.42 0.47 0.57 0.42 0.63 0.63

C4-chrysenes 0.41 0.31 0.44 0.14 0.37 1.31 0.57 1.23
37

Table 11 (cont.). Crude Oil (QA10OIL01): p-scores (15%) - Alkylated PAHs 

(p=1 reported rsd is 15%; p=2 reported rsd is 30%)

Laboratory No. 14 15 16 17 18 19 20 21 22 23 24 25 26

1-methylnaphthalene 0.13 0.09 0.38 1.62 0.04 0.65 0.65 0.34 0.19 0.04 0.27 0.61

2-methylnaphthalene 0.18 0.09 0.40 0.11 0.05 0.72 0.72 0.27 0.22 0.02 0.09 0.25

2,6-dimethylnaphthalene 0.15 0.08 0.21 0.03 0.67 0.51 0.23 0.29 0.05 0.10 0.39 0.00

1,6,7-trimethylnaphthalene 0.16 0.08 0.04 0.39 0.88 0.33 0.20 0.72 0.97

1-methylphenanthrene 0.49 0.44 0.40 0.19 0.04 0.62 0.18 0.40 0.25 0.11 0.25 0.21 0.37

C1-decalins 0.37 0.47

C2-decalins 0.45 0.37

C3-decalins 0.56 0.27

C4-decalins 0.10 0.46 0.33

C1-naphthalenes 0.24 0.07 0.47 0.24 0.72 0.70 0.30 0.21 0.03 0.08 0.42

C2-naphthalenes 0.35 0.11 0.66 0.43 0.75 0.48 0.24 0.28 0.05 0.50 0.21

C3-naphthalenes 0.14 0.10 0.56 0.20 0.75 1.13 0.38 0.30 0.02 0.22 0.31

C4-naphthalenes 0.11 0.05 0.56 2.56 0.09 0.92 1.15 0.44 0.32 0.05 0.90 0.95

C1-benzothiophenes 1.18 0.12 0.27 0.13

C2-benzothiophenes 1.74 0.35 0.26 0.19

C3-benzothiophenes 0.79 0.13 0.27 0.29

C4-benzothiophenes 0.22 0.35 0.43

C1-fluorenes 0.12 0.07 0.49 0.69 0.16 0.50 0.84 0.13 0.30 0.03 0.20 0.18

C2-fluorenes 0.20 0.10 1.01 1.81 1.02 0.40 0.55 0.26 0.11 0.59 0.14

C3-fluorenes 0.31 0.26 0.81 0.80 0.14 0.70 0.59 0.18 0.34 0.20 1.00 0.30

C1-phenanthrenes/anthracenes 0.21 0.17 0.44 0.35 0.77 0.15 0.11 0.31 0.08 0.19 0.29

C2-phenanthrenes/anthracenes 0.14 0.16 0.44 0.43 0.08 0.65 0.48 0.08 0.33 0.15 0.17 0.25

C3-phenanthrenes/anthracenes 0.30 0.19 0.00 0.23 0.13 0.66 1.09 0.22 0.38 0.22 0.39 0.22

C4-phenanthrenes/anthracenes 0.17 0.33 0.00 1.86 0.19 0.60 0.69 0.13 0.45 0.32 0.42 0.22

C1-dibenzothiophenes 0.30 0.67 0.61 0.24 0.39 0.87 0.12 0.03 0.37 0.05 0.52 0.28

C2-dibenzothiophenes 0.15 0.03 0.54 0.99 0.68 0.24 0.05 0.27 0.08 0.52 0.68

C3-dibenzothiophenes 0.22 0.33 1.34 0.88 0.37 0.42 0.12 0.79 0.82

C4-dibenzothiophenes 0.55 0.34 2.09 0.13 1.60 0.42 0.27 0.69 0.48

C1-fluoranthenes/pyrenes 0.24 0.17 2.64 0.19 1.29 0.08 0.64 0.36 0.23 0.54 0.55

C2-fluoranthenes/pyrenes 0.17 0.15 0.04 0.83 1.02 0.37 0.38 0.61 0.61

C3-fluoranthenes/pyrenes 0.17 0.43 0.41 1.17 0.28 0.51 0.35 0.67 0.56

C4-fluoranthenes/pyrenes 3.57 0.09 0.81 0.41 0.47 0.73 0.50

C1-naphthobenzothiophenes 1.15 0.08 0.45 0.43 0.26

C2-naphthobenzothiophenes 0.06 0.86 0.42 0.24

C3-naphthobenzothiophenes 0.42 0.14 0.46 0.22

C4-naphthobenzothiophenes 0.25 0.42 0.88

C1-chrysenes 0.18 0.09 0.25 0.16 0.14 0.77 0.45 0.83 0.30 0.15 0.03 0.11

C2-chrysenes 0.23 0.34 1.38 0.89 0.95 0.33 0.28 0.27 0.36 0.34

C3-chrysenes 0.22 1.51 0.04 0.74 1.21 0.57 0.21 0.75 0.52 0.15

C4-chrysenes 0.19 0.23 0.97 0.27 1.60 0.11 0.28
38

Table 12. Crude Oil (QA10OIL01): p-scores (15%) - Biomarkers

(p=1 reported rsd is 15%; p=2 reported rsd is 30%)

Laboratory No. 1 2 3 4 5 6 7 8 9 10 11 12 13

Carbazole 1.15 0.91 0.48

18a(H)-22,29,30-Trisnorneohopane 0.41 1.11 0.27 0.10

17a(H)-22,29,30-Trisnorhopane 0.23 0.42 2.66 0.42 0.19 0.41

17α(H),21β(H)-30-Norhopane 0.62 0.52 4.76 0.45 0.00

18a(H)-30-Norneohopane 0.33 5.31 0.32 0.45

17a(H)-Diahopane 0.23 2.61 1.25 1.05

17α(H),21β(H)-Hopane 0.34 0.51 0.45 0.80 0.59 0.21 0.55

17α(H),21β(H)-22R-Homohopane 0.52 0.61 2.04 0.75 0.34

17α(H),21β(H)-22S-Homohopane 0.23 0.39 2.73 0.51 0.26

13b(H)17a(H)-Diacholestane 20S 0.21 0.37 0.43 0.25

5a(H),14a(H),17a(H)-Cholestane 20S 0.51 0.80 0.10

5a(H),14a(H),17a(H)-Cholestane 20R 0.22 1.76 1.12 0.30 0.13 2.02

5a(H),14a(H),17a(H)-24-Ethylcholestane 20S 0.27 3.36 0.41 0.44

5a(H),14a(H),17a(H)-24-Ethylcholestane 20R 0.29 0.39 1.26 0.92 0.19

5a(H),14b(H),17b(H)-Cholestane 20R 0.30 0.39 0.63 0.42 0.22

5a(H),14b(H),17b(H)-Cholestane 20S 0.38 1.39 0.92 0.23

5a(H),14b(H),17b(H)-24-Ethylcholestane 20R 0.28 0.54 2.07 0.38 0.33

5a(H),14b(H),17b(H)-24-Ethylcholestane 20S 1.65 1.34 0.81
39

Table 12 (cont). Crude Oil (QA10OIL01): p-scores (15%) - Biomarkers

(p=1 reported rsd is 15%; p=2 reported rsd is 30%)

Laboratory No. 14 15 16 17 18 19 20 21 22 23 24 25 26

Carbazole 0.73 0.42

18a(H)-22,29,30-Trisnorneohopane 0.10 1.76 0.35 0.50

17a(H)-22,29,30-Trisnorhopane 0.34 2.27 0.61 0.39 0.32 0.34

17α(H),21β(H)-30-Norhopane 0.37 1.36 0.76 1.28 0.38 0.34 0.69

18a(H)-30-Norneohopane 0.30 1.51 0.00 0.50 0.27

17a(H)-Diahopane 0.18 1.54 0.64 0.00 0.00

17α(H),21β(H)-Hopane 0.23 0.61 1.43 0.55 0.35 0.49

17α(H),21β(H)-22R-Homohopane 0.27 0.03 1.35 0.29 0.31 0.61

17α(H),21β(H)-22S-Homohopane 0.25 1.27 1.54 0.48 0.36 0.67

13b(H)17a(H)-Diacholestane 20S 0.07 0.28 1.53 0.44 0.00

5a(H),14a(H),17a(H)-Cholestane 20S 0.29 0.31 1.60 0.38 0.40

5a(H),14a(H),17a(H)-Cholestane 20R 0.19 0.84 0.57 1.40 0.43 0.42 0.25

5a(H),14a(H),17a(H)-24-Ethylcholestane 20S 0.49 0.78 0.10 0.39

5a(H),14a(H),17a(H)-24-Ethylcholestane 20R 0.27 1.22 0.84 0.42 0.20

5a(H),14b(H),17b(H)-Cholestane 20R 0.34 0.98 1.81 0.52 0.44 0.43

5a(H),14b(H),17b(H)-Cholestane 20S 0.62 1.70 2.02 0.51 0.38

5a(H),14b(H),17b(H)-24-Ethylcholestane 20R 0.26 0.47 0.78 0.73 0.52 0.29 0.31

5a(H),14b(H),17b(H)-24-Ethylcholestane 20S 0.64 n=1 1.32 1.24 0.52
40

APPENDIX A

Interlaboratory Analytical Comparison Study to Support Deepwater Horizon Natural Resource 

Damage Assessment

Intercomparison Exercise: Crude Oil QA10OIL01

Description of Materials and Instructions

Intercomparison Exercise Material:

Each of the five ampoules contains approximately 1.3 mL of Crude Oil QA10OIL01. This crude oil 

was collected from the site of the Deepwater Horizon oil rig on May 21, 2010. This material was not 

enriched or spiked. Each ampoule is labeled with an individual ampoule number as well as the above 

name.

In addition, three concurrent analyses of SRM 1582 Petroleum Crude Oil are recommended. This 

material can be obtained from the NIST Standard Reference Materials Program ($309/5 x 5 mL 

ampoules). See the following link for information on ordering on-line: https://wwws.nist.gov/srmors/view_detail.cfm?srm=1582.

Storage of Materials:

Sealed ampoules, as received, should be stored in the dark at temperatures between 10 °C and 30 °C. 

Instructions for Use:

You are to analyze Crude Oil QA10OIL01 and SRM 1582 using your laboratory's and/or program's 

analytical protocols, for the concentrations (mass/mass) of the parent polycyclic aromatic hydrocarbon 

(PAH) compounds, alkylated PAH compounds, and biomarkers currently being determined in your 

laboratory. A target list of compounds are presented in Table 1; however, participants do not need to 

quantify all of these compounds and can add additional compounds when reporting the data. 

The amount of material used for each analysis should correspond to the amount of crude that you would 

typically analyze as prescribed in your protocols. Prior to opening the ampoules of QA10OIL01, you 

should sonicate the sample in the ampoule. Samples for analysis should be withdrawn immediately 

after opening the ampoule and should be processed without delay. Material should not be stored in the 

ampoules that have been opened, even if they are resealed.

You should analyze three samples of Crude Oil QA10OIL01 and at least one or more samples of SRM 

1582 using your protocol for crude oil samples. If time allows, we are asking that you analyze one

sample of Crude Oil QA10OIL01 and one sample of SRM 1582 with one batch of laboratory samples; 

analyze a second sample of each material with another batch; and the third sample with yet another 

batch. This will allow a more realistic assessment of laboratory precision over a longer term than the 

assessment obtained when a laboratory places all three samples in the same batch and the resulting 

extracts are analyzed using the same calibration curve, etc. 
41

Reporting of Results:

Please report one result, as if three figures were significant, for each of the analytes quantified in each 

of the three replicates of the Crude Oil QA10OIL01 and of SRM 1582. Report results in units of μg/g. 

Report the date of measurement of each sample in the requested m/d/y format. 

If you know that a target or non-target compound is interfering (coeluting) with the determination of a 

target analyte, please identify this issue by qualifying the data and note the data qualifier used at the 

bottom of your table of results. Please note that any changes you make to the column or row headings 

within the tables will not be seen by the coordinators because only the table entries and comments at 

the bottom of the tables are automatically transferred to the exercise database.

We prefer that concentration values be reported for each analyte determined. If the measured 

concentration is below your typical reporting concentration for an analyte in a particular matrix, you 

can report the number and list the appropriate detection limit, quantification limit, etc. at the bottom of 

the data table. However, if you need to report non-numerical data please use the following 

conventions:

NA "Not analyzed", "not determined"

<"value" "Less than specified concentration", e.g., <8 μg/g

Other "Other"; add note of explanation at end of data table, e.g., interference

DL "Below detection limit" may be used, however, <"value" is preferable

Do not use negative numbers or parentheses to indicate "less than detection limits". 

The attached file is an EXCEL file, QA10OIL01.xls. If you have any software/hardware conversion 

problems, please contact Michele Schantz. The data file templates also include places for you to list the 

surrogate/internal standards and type of calibration curve used, and to provide a brief description of the 

analyses. Please do not add spaces before entering numbers in the table cells and enter them as 

"numbers" not as "labels". Please do not insert any columns or rows within the table in the data file. If 

you wish to include additional data and/or other information or comments, you may add it to the bottom 

of the data table in the attached file. 

Submit your results by January 21, 2011 as an attached file via e-mail to: 

michele.schantz@nist.gov 

Further Information:

If you need further information, please contact Michele at the following address or phone numbers:

Michele M. Schantz

NIST

100 Bureau Drive Stop 8392

Gaithersburg, MD 20899-8392 

Phone: (301)975-3106 FAX: (301)977-0685
42

Table 1: Preliminary List of Analytes of Interest in the Interlaboratory Analytical 

Comparison Study to Support Deepwater Horizon Natural Resource Damage 

Assessment

PAHs

Naphthalene cis/trans-Decalin

Biphenyl Dibenzofuran

Acenaphthene Retene

Acenaphthylene Benzothiophene

Fluorene Dibenzothiophene

Phenanthrene Naphthobenzothiophene

Anthracene

Fluoranthene

Pyrene

Benzo[b]fluorene

Benz[a]anthracene

Chrysene

Triphenylene

Benzo[b]fluoranthene

Benzo[j]fluoranthene

Benzo[k]fluoranthene

Benzo[a]fluoranthene

Benzo[e]pyrene

Benzo[a]pyrene

Perylene

Indeno[1,2,3-cd]pyrene

Benzo[ghi]perylene

Dibenz[a,h]anthracene

Alkylated PAHs

1-Methylnaphthalene C1-Naphthalenes

2-Methylnaphthalene C2-Naphthalenes

2,6-Dimethylnaphthalene C3-Naphthalenes

1,6.7-Trimethylnaphthalene C4-Naphthalenes

1-Methylphenanthrene

C1-Benzothiophenes

C1-Decalins C2-Benzothiophenes

C2-Decalins C3-Benzothiophenes

C3-Decalins C4-Benzothiophenes

C4-Decalins
43

Table 1 (cont.)

C1-Fluorenes C1-Naphthobenzothiophenes

C2-Fluorenes C2-Naphthobenzothiophenes

C3-Fluorenes C3-Naphthobenzothiophenes

C4-Naphthobenzothiophenes

C1-Phenanthrenes/anthracenes

C2- Phenanthrenes/anthracenes C1-Chrysenes

C3- Phenanthrenes/anthracenes C2-Chrysenes

C4- Phenanthrenes/anthracenes C3-Chrysenes

C1-Dibenzothiophenes C4-Chrysenes

C2-Dibenzothiophenes

C3-Dibenzothiophenes

C4-Dibenzothiophenes

C1-Fluoranthenes/pyrenes

C2-Fluoranthenes/pyrenes

C3-Fluoranthenes/pyrenes

C4-Fluoranthenes/pyrenes

Biomarkers

Carbazole

18α(H)-22,29,30-Trisnorneohopane

17α(H)-22,29,30-Trisnorhopane

17α(H),21β(H)-30-Norhopane

18α(H)-30-Norneohopane

17α(H)-Diahopane

17α(H),21β(H)-Hopane

17α(H),21β(H)-22R-Homohopane

17α(H),21β(H)-22S-Homohopane

13β(H) 17α(H)-Diacholestane 20S

5α(H),14α(H),17α(H)-Cholestane 20S

5α(H),14α(H),17α(H)-Cholestane 20R

5α(H),14α(H),17α(H)-24-Ethylcholestane 20S

5α(H),14α(H),17α(H)-24-Ethylcholestane 20R

5α(H),14β(H),17β(H)-Cholestane 20S

5α(H),14β(H),17β(H)-Cholestane 20R

5α(H),14β(H),17β(H)-24-Ethylcholestane 20S

5α(H),14β(H),17β(H)-24-Ethylcholestane 20R
44

Appendix B

Summary of Method Information Provided by the Participating Laboratories
45

METHOD Information as Provided by Laboratories

g used g used Method of 

Lab # Reported QA10OIL01 SRM 1582 Sample cleanup method quantitation

1 1/31/2011 0.4 0.4

Add IS solution and dilute to approx. 2 mL; Take 0.5 mL for aminopropyl Sep Pak (SPE) - conditioning and eluting with 20 mL of 5% 

dichloromethane (DCM ) in hexane; Concentrate to approx. 0.3 mL and inject in LC using semi-preparative scale aminopropyl silane column - 

collect 3 fractions - 1st with aliphatics and hopanes/steranes; 2nd with naphthalene through dimethylphenanthrenes; 3rd with remainder of PAHs 

through 302's - mobile phase is 2% DCM in hexane for 1st 145 mL and 20% DCM in hexane for remainder IS

2 12/28/2010 0.1 A potion of the crude oil sample was measured, mixed into DCM, and analyzed via direct injection GC/MS. IS

3 1/14/2011 0.40 0.04 NA IS

4 1/19/2011 0.10 0.10 Silica gel cleanup performed. IS

5 1/17/2011 1 1 none IS

6 1/20/2011 1.00 1.00 EPA 3540A Waste Dilution, with no cleanup IS

7 1/21/2011 1.000 1.000

Intercalibration Oil:1/100 dilution of the oils and direct injection after spiking with surrogate and internal standards; SRM 1582:1/1000 dilution of the SRM and direct injection 

after spiking with surrogate and internal standards

Surrogate 

Standards

8 1/21/2011 1.00 0.01 SW-846 method 3580A IS

9 1/21/2011 0.12 0.12 Gravity flow column with silica gel and neutral alumina, followed by size-exclusion HPLC to elute fraction containing PAHs and alkyl-PAHs. IS

10 1/20/2011 0.00300 0.00180 None IS

11 1/14/2011 0.10 0.10

Samples are diluted with DCM, spiked with labeled surrogate, cleaned up using a 2.5 g (2% deactivated) neutral alumina/5g silica column. 

Fraction 1 is eluted off with pentane. Fraction 2 is eluted with 60:40 DCM:Hexane. Fraction 1 and 2 are analysed in two separate runs for 

biomarkers. Fraction 1 and 2 are combined to analyze for PAHs and alkylated PAHs. IS

12 1/21/2011 0.055 0.055 none IS

13 1/21/2011 0.0850 0.0870 Samples diluted in DCM IS

14 1/21/2011 0.0260 0.0260 SW-846 3580A Waste Dilution IS

15 1/21/2011 0.05 0.05 IS

16 1/21/2011 0.1 0.1 Mercury cleanup was performed to reduce sulphur interference. IS

17 1/21/2011 1.0 0.1 &10

EPA Method 3580A- Approximately 1.0 g of each sample was weigh into 10.0 mL volumetric flask and and surrogate spiking solution was 

added to all samples and QC Samples before diluted to 10.0 mL final volume. Similarly, 1.0 g of the reference standard SRM 1582 was diluted 

with DCM into volumetric flask Again 2.0 ml of surrogate spking solution was added before the extraction process. IS

18 1/21/2011 1 0.2 EPA 3580A; EPA 3630 Silica gel IS

19 1/21/11 0.100 0.100 SW 3580 (Waste Dilution) IS

20 1/21/2011 0.9 0.9 None. IS

21 1/21/2011 0.002 to 0.005 0.002 to 0.01

Stock solutions of the oils (1 g in 50 mL) were prepared in DCM to produce concentrations around 20,000ppm. Dilutions of the stock were 

suplemented with the appropiate surrogate and recovery standards and analyzed by GC/MS-SIM withouth further cleanup. IS

22 1/28/2011 ~0.1 ~0.1 NA IS

23 2/2/2011 0.0046 0.0046 Dissolved sample eluted from 6 g silica gel column with 1:1 volume of pentane:DCM solution. IS

24 1/31/2011 0.005 0.005

Samples were diluted in DCM, aliquoted, and solvent exchanged to hexane using an N-Evap nitrogen blow-down. Aliquots equivalent to 

approximately 0.01 g or 0.001 g oil were eluted through 2 g 6% deactivated alumina with petroleum ether and toluene. Samples were then 

concentrated and solvent exhanged to hexane for GCMS analysis. IS

25 2/11/2011 0.025 0.035

Oil samples were diluted approximately 1/25 (mass:mass) with hexane. 0.5 mL of this solution was combined gravimentrically with an internal 

standard solution containing deuterated PAHs and deuterated aliphatic hydrocarbons. The solution was then separated using an alumina:silica 

column containing 2 g of alumina (6% detactivated with water) and 3 g of silica dectivated with 50 uL of water. The oil dilution was added and 

two fractions were collected: fraction 1 was 8 mL of hexane and fraction 2 was 20 mL 20% DCM:hexane (volume fraction). IS

26 2/11/2011 0.1 0.1 Silica Gel Cleanup IS
46

Analytical Methods used for PAHs

PAHs Calibration Curve

Lab # Instrument Phase Dimensions injection mode # points range*

1 GC/MS DB-17MS 60m x 0.25mm, 0.25μm film on-column 6 varied by compound

2 GC/MS DB-5.625 20m x 0.18mm, 0.36μm film splitless 5 500 ug/g to 8000 ug/g

3 GC/MS ZB-5MSi 60m x 0.25mm, 0.25μm film splitless 6 0.008 ug/mL to 0.80 ug/mL

4 MS04 Rtx-5MS 30m x 0.32mm, 0.25μm film split 7 4ug/g to 1000ug/g

5 MSD7 ZB-MS-5si 60m x 0.25mm, 0.25μm film splitless 6 0.1 ug/mL to 4.0 ug/mL

6 GC/MS DB-5 30m x 0.25mm, 0.25μm film splitless 5 10 ng/mL to 1000 ng/mL

7 GC/MS DB5-MS 30m x 0.25mm, 0.25μm film split/splitless 5 20 ng/mL to 1000 ng/mL

8 GC/MS DB - 5 60m x 0.25mm, 0.25μm film splitless 6 0.1 ug/mL to 4.0 ug/mL

9 GC/MS DB-5 60m x 0.25mm, 0.25μm film on-column 9 0.001 ng/μL to 10.0 ng/μL

10 GC/MS HP-5MS 60m x 0.25mm, 0.25μm film splitless 5 (20, 100, 250, 500,1000) ng/mL

11 GC/MS DB-5 30m x 0.25mm, 0.25μm film splitless 6 0.05 mg/L to 5 mg/L

12 GC-MS DB-5 60m x 0.25mm, 0.25μm film splitless 7 ~0.01 ng/uL to 7 ng/uL

13 GC/MS Rtx-5MS 60m x 0.25mm, 0.25μm film splitless 6 130 ug/L to 50,000 ug/L

14 GC/MS 7890A ZB5MSi 60m x 0.25mm, 0.25μm film 6 0.02 ug/L to 0.8 ug/L

15 MSE ZB-5MSI 60m x 0.25mm, 0.25μm film split/splitless 6 0.1 ppm to 4.0ppm

16 GC/MS ZB-MS-5Si 60m x 0.25mm, 0.25μm film pulsed splittless 6 0.1 ug/mL to 4.0 ug/mL

17 GC/MS

5% diphenyl / 95% 

dimethyl polysiloxane 30m x 0.25mm, 0.25μm film 5 2ug/ml to 50ug/mL

18 GC/MS ZB-5MS 30m x 0.25mm, 0.25μm film 10 2 ng/L to 2000 ng/L

19 GC/MS SIM DB5MS 30m x 0.25mm, 0.5μm film split 8

0.13 ug/kg to 23.33 ug/kg (0.04 ug/mL 

to 7 ug/mL)

20 GC/MS ZB5-MSI 60m x 0.25mm, 0.25μm film splitless 6 0.1 ug/mL to 4 ug/mL

21 THERMO DSQII RTX5-MS 30m x 0.25mm, 0.25μm film splitless (2 ul) 6 0.020 ng/uL to 4.000 ng/uL

22 GC/MS-SIM ZB-5 60m x 0.25mm, 0.25μm film splitless 7 10 ng/mL to 20,000ng/mL

23 GC/MS

5% phenylmethyl 

silicone 25m x 0.2mm, 0.33μm film splitless 5 15 ng/mL to 700 ng/mL

24 GC/MS DB5 30m x 0.25mm, 0.25μm film PTV 6 10ng/mL to 5000ng/mL

25 GC/MS Rxi-17 sil 60m x 0.25mm, 0.25μm film splitless 6 2 ng/mL to 2000 ng/mL

26 GC/MS ZB-5MS 30m x 0.25mm, 0.25μm film split/splitless 7 2 ppb to 2000ppb

* Note that units are those provided by the participating laboratory.
47

Analytical Methods used for Alkylated PAHs

Alkylated PAH Calibration Curve

Lab # Instrument Phase Dimensions injection mode # points range*

1 GC/MS DB-17MS 60m x 0.25mm, 0.25μm film on-column 6 varied by compound

2 GC/MS DB-5.625 20m x 0.18mm, 0.36μm film splitless 5 500 - 8000 ug/g

3 GC/MS ZB-5MSi 60m x 0.25mm, 0.25μm film splitless 6 0.008-0.80 ug/mL

4 MS04 Rtx-5MS 30m x 0.32mm, 0.25μm film split 7 4ug/g to 1000ug/g

5 MSD7 ZB-MS-5si 60m x 0.25mm, 0.25μm film splitless 6 0.1 ug/mL to 4.0 ug/mL

6 GC/MS DB-5 30m x 0.25mm, 0.25μm film splitless 5 10 ng/ml to 1000 ng/ml

7 GC/MS DB5-MS 30m x 0.25mm, 0.25μm film split/splitless 5 20 ng/mL to 1000 ng/mL

8 GC/MS DB - 5 60m x 0.25mm, 0.25μm film splitless 6 0.1 ug/mL to 4.0 ug/mL

9 GC/MS DB-5 60m x 0.25mm, 0.25μm film on-column 9 0.001 ng/μL to 10.0 ng/μL

10 GC/MS HP-5MS 60m x 0.25mm, 0.25μm film splitless 5 (20, 100, 250, 500,1000) ng/mL

11 GC/MS DB-5 30m x 0.25mm, 0.25μm film splitless 6 0.05 mg/L to 5 mg/L

12 GC-MS DB-5 60m x 0.25mm, 0.25μm film splitless 7 ~0.01 ng/uL to 7 ng/uL

13 GC/MS Rtx-5MS 60m x 0.25mm, 0.25μm film splitless 6 130 ug/L to 50,000 ug/L

14 GC/MS 7890A ZB5MSi 60m x 0.25mm, 0.25μm film 6 0.02 ug/L to 0.8 ug/L

15 MSE ZB-5MSI 60m x 0.25mm, 0.25μm film split/splitless 6 0.1 ppm to 4.0ppm

16 GC/MS ZB-MS-5Si 60m x 0.25mm, 0.25μm film pulsed splittless 6 0.1 ug/mL to 4.0 ug/mL

17 GC/MS

5% diphenyl / 95% 

dimethyl polysiloxane 30m x 0.25mm, 0.25μm film 5 2ug/ml to 50ug/mL

18 GC/MS ZB-5MS 30m x 0.25mm, 0.25μm film 10 2 ng/L to 2000 ng/L

19 GC/MS SIM DB5MS 30m x 0.25mm, 0.5μm film split 8

0.13 ug/kg to 23.33 ug/kg (0.04 ug/mL 

to 7 ug/mL)

20 GC/MS ZB5-MSI 60m x 0.25mm, 0.25μm film splitless 6 0.1 ug/mL to 4 ug/mL

21 THERMO DSQII RTX5-MS 30m x 0.25mm, 0.25μm film splitless (2 ul) 6 0.020 ng/uL to 4.000 ng/uL

22 GC/MS-SIM ZB-5 60m x 0.25mm, 0.25μm film splitless 7 10 ng/mL to 20,000ng/mL

23 GC/MS

5% phenylmethyl 

silicone 25m x 0.2mm, 0.33μm film splitless 5 15 ng/mL to 700 ng/mL

24 GC/MS DB5 30m x 0.25mm, 0.25μm film PTV 6 10ng/mL to 5000ng/mL

25 GC/MS Rxi-17 sil 60m x 0.25mm, 0.25μm film splitless 6 0.6 ng/mL to 600 ng/mL

26 GC/MS ZB-5MS 30m x 0.25mm, 0.25μm film split/splitless

* Note that units are those provided by the participating laboratory.
48

Analytical Methods used for Biomarkers

Biomarkers Calibration Curve

Lab # Instrument Phase Dimensions injection mode # points range*

1 GC/MS DB-17MS 60m x 0.25mm, 0.25μm film on-column 6 varied by compound

2 GC/MS DB-5.625 20m x 0.18mm, 0.36μm film splitless 5 500 - 8000 ug/g

3 GC/MS ZB-5MSi 60m x 0.25mm, 0.25μm film splitless 6 0.008-0.80 ug/mL

4 MS04 Rtx-5MS 30m x 0.32mm, 0.25μm film split 7 4ug/g to 1000ug/g

5 MSD7 ZB-MS-5si 60m x 0.25mm, 0.25μm film splitless 6 0.1 ug/mL to 4.0 ug/mL

6 GC/MS DB-5 30m x 0.25mm, 0.25μm film splitless 5 10 ng/ml to 1000 ng/ml

7 NA NA NA NA NA NA

8 GC/MS DB - 5 60m x 0.25mm, 0.25μm film splitless 6 0.1 ug/mL to 4.0 ug/mL

9 NA NA NA NA NA NA

10 NA NA NA NA NA NA

11 GC/MS DB-5 60m x 0.25mm, 0.25μm film splitless NA NA

12 GC-MS DB-5 60m x 0.25mm, 0.25μm film splitless 7 ~0.01 ng/uL to 7 ng/uL

13 GC/MS Rtx-5MS 60m x 0.25mm, 0.25μm film splitless 6 300 ug/L to 30,000 ug/L

14 GC/MS 7890A ZB5MSi 60m x 0.25mm, 0.25μm film 6 0.02 ug/L to 0.8 ug/L

15 MSE ZB-5MSI 60m x 0.25mm, 0.25μm film split/splitless 6 0.1 ppm to 4.0ppm

16 GC/MS ZB-MS-5Si 60m x 0.25mm, 0.25μm film pulsed splittless 6 0.1 ug/mL to 4.0 ug/mL

17 GC/MS

5% diphenyl / 95% 

dimethyl polysiloxane 30m x 0.25mm, 0.25μm film 5 2ug/ml to 50ug/mL

18

19 GC/MS SIM DB5MS 30m x 0.25mm, 0.5μm film split 8

0.13 ug/kg to 23.33 ug/kg (0.04 

ug/mL to 7 ug/mL)

20 GC/MS ZB5-MSI 60m x 0.25mm, 0.25μm film splitless 6 0.1 ug/mL to 4 ug/mL

21 NA NA NA NA NA NA

22 GC/MS-SIM ZB-5 60m x 0.25mm, 0.25μm film splitless 6 10 ng/mL to 10,000ng/mL

23 GC/MS

5% phenylmethyl 

silicone 25m x 0.2mm, 0.33μm film splitless 5 10 ng/mL to 5000 ng/ml

24 NA NA NA NA NA NA

25 GC/MS Rxi-17 sil 60m x 0.25mm, 0.25μm film splitless 6 0.4 ng/mL to 2400 ng/mL

26

* Note that units are those provided by the participating laboratory.
49

Qunatitation of PAHs

PAHs corrected for

Lab # IS/surrogate added prior to extraction Used? added prior to analysis Used? recovery? others?

1

naphthalene-d8;biphenyl-d10; acenaphthalene-d10; phenanthrene-d10; fluoranthene-d10; pyrene-d10; B[a]Ad12; B[a]P-d12; perylene-d12; B[ghi]P-d12;DB[a,h]A-d14 x

2 naphthalene-d8, acenaphthene-d10, phenanthrene-d10, chrysene-d12, perylene-d12 x no

3 Fluorene-d10, Benzo(a)pyrene-d12 x no

4 Naphthalene-d8, Acenaphthene-d10, Phenanthrene-d10, 5 alpha-Androstane, Chrysene-d12, Perylene-d12 Fluorene-d10, Pyrene-d10, Benzo(a)pyrene-d12 x yes

5 Nitrobenzene- d5; 2- Fluorobiphenyl; Terphenyl- d14 Naphthalene- d8; Acenapthene- d10; Chrysene- d12; Perylene- d12 x yes

6

Acenaphthene-d10, Acenaphthylene-d8, Anthracene-d10, Benz[a]Anthracene-d12, Benzo[b]Fluoranthene-d12, 

Benzo[k]Fluoranthene-d12, Benzo[g,h,i]Perylene-d12, Benzo[a]Pyrene-d12, Chrysene-d12, 

Dibenz[a,h]Anthracene-d14, Fluoranthene-d10, Fluorene-d10, Indeno[l,2,3-cd]Pyrene-d12, Naphthalene-d8, 

Phenanthrene-d10, Pyrene-d10 x no

7 d8-Naphthalene, d10-Acenaphthene, d10-Phenanthrene, d12-Chrysene, d12-Perylene x d10-Fluorene, d12-Benzo(a)pyrene

8 Nitrobenzene-d5, 2-Fluorobiphenyl, Terphenyl-d14 Naphthalene-d8, Acenaphthene-d10, Phenanthrene-d10, Chrysene-d10 x no

9 Naphthalene-d8, Acenaphthene-d10, Benzo[a]Pyrene-d12 x Hexamethylbenzene

Phenanthrene-d10 - prior to size-exclusion 

HPLC cleanup

10 SU-Naphthalene-d8, Acenaphthene-d10, Phenanthrene-d10, Chrysene-d12, Perylene-d12 IS-Fluorene-d10, Pyrene-d10, Benzo(a)pyrene-d12 x yes

11

phenanthrene d10, benzo(a)pyrene d12, benzo(ghi)perylene d12, naphthalene d8, acenaphthylene d8, pyrene 

d10 2-fluorobiphenyl, p-terphenyl-d14, chrysene d12, dibenzo(a,h)anthracene d14 x no

12 Naphthalene-d8, Acenaphthene-d10, Phenanthrene-d10, Benzo[a]pyrene-d12 Fluorene-d10, Chrysene-d12 x yes

13 Nitrobenzene d5/p Terphenyl d14/2-Fluorobiphenyl 1,4 Dichlorobenzene d4/Naphthalene d8/Acenaphthene d10/Phenanthrene d10/Chrysene d12/Perylene d12 x no

14 Surrogates: Nitrobenzene-d5; 2-Fluorobiphenyl; Terphenyl-d14 Naphthalene-d8; Acenaphthene-d10; Phenanthrene-d10; Chrysene-d12; Perylene-d12 no

15 Surr IS x no

16 2-Fluorobiphenyl, Nitrobenzene-d5, Terphenyl-d14 Acenaphthene-d10, Chrysene-d12, Naphthalene-d8, Perylene-d12, Phenanthrene-d10 x yes

17 Nitrobenzene d5/p Terphenyl d14/2-Fluorobiphenyl 1,4 Dichlorobenzene d4/Naphthalene d8/Acenaphthene d10/Phenanthrene d10/Chrysene d12/Perylene d12 x no

18 Naphthalene-d8, Acenaphthene-d10, Phenanthrene-d10, Chrysene-d12, Pyrene-12 x yes

19 Nitrobenzene-d5 (S), 2-Fluorobiphenyl (S), and Terphenyl-d14 (S)

Naphthalene-d8 (IS), Acenaphthalene-d10 (IS), Phenanthrene-d10 (IS), Chrysene-d12 (IS), and Perylene-d12 

(IS) x no

20 PAH0235 (Surr) SVBIOM ISTD-00001 (IS) x no

21 naphthalene-d8, acenaphtene-d10, phenenthrene-d10, chrysene-d12, and perylene-d12 x fluorene-d10, benzo(a)pyrene-d12

22 Surrogates x IS x yes

23 d8Naph, d10Ace, d10Phen, d12Chry, d12B-a-P, d12Pery x hexamethylbenzene

24

d8-Napthalene, d8-1-Methylnapthalene, d8-Acenaphthylene, d10-Acenaphthene, d10-Fluorene, d10-

Phenanthrene, d10-Anthracene, d10-Fluoranthene, d10-Pyrene, d12-Benz(a)anthracene, d12-Chrysene, d12-

Benzo(b)fluoranthene, d12-Benzo(k)fluoranthene, d12-Benzo(e)pyrene, d12-Benzo(a)pyrene, d12-Perylene, 

d12-Benzo(g,h,i)perylene x d14-p-Terphenyl

25

Naphthalene-d8; Acenaphthene-d10; Phenanthrene-d10; Fluoranthene-d10; Pyrene-d10; Benz[a]anthracened12; Benzo[a]pyrene-d12; Perylene-d12; Bibenz[a,h]anthracene-d14; Benzo [[ghi]perylene-d12 x

26 surrogates only Internal standards x no
50

Qunatitation of Alkylated PAHs

Alkylated PAHs corrected for

Lab # IS/surrogate added prior to extraction Used? added prior to analysis Used? recovery? others?

1

naphthalene-d8;biphenyl-d10; acenaphthalene-d10; phenanthrene-d10; fluoranthene-d10; pyrene-d10; B[a]Ad12; B[a]P-d12; perylene-d12; B[ghi]P-d12;DB[a,h]A-d14 x

2 naphthalene-d8 x no

3 Fluorene-d10, Benzo(a)pyrene-d12 x no

4 Naphthalene-d8, Acenaphthene-d10, Phenanthrene-d10, 5 alpha-Androstane, Chrysene-d12, Perylene-d12 Fluorene-d10, Pyrene-d10, Benzo(a)pyrene-d12 x yes

5 Nitrobenzene- d5; 2- Fluorobiphenyl; Terphenyl- d14 Naphthalene- d8; Acenapthene- d10; Chrysene- d12; Perylene- d12 x yes

6

7 d8-Naphthalene, d10-Acenaphthene, d10-Phenanthrene, d12-Chrysene, d12-Perylene x d10-Fluorene, d12-Benzo(a)pyrene

8 Nitrobenzene-d5, 2-Fluorobiphenyl, Terphenyl-d14 Naphthalene-d8, Acenaphthene-d10, Phenanthrene-d10, Chrysene-d10 x no

9 Naphthalene-d8, Acenaphthene-d10, Benzo[a]Pyrene-d12 x Hexamethylbenzene

Phenanthrene-d10 - prior to size-exclusion 

HPLC cleanup

10 SU-Naphthalene-d8, Acenaphthene-d10, Phenanthrene-d10, Chrysene-d12, Perylene-d12 IS-Fluorene-d10, Pyrene-d10, Benzo(a)pyrene-d12 x yes

11

phenanthrene d10, benzo(a)pyrene d12, benzo(ghi)perylene d12, naphthalene d8, acenaphthylene d8, pyrene 

d10 2-fluorobiphenyl, p-terphenyl-d14, chrysene d12, dibenzo(a,h)anthracene d14 x no

12 Naphthalene-d8, Acenaphthene-d10, Phenanthrene-d10, Benzo[a]pyrene-d12 Fluorene-d10, Chrysene-d12 x yes

13 Nitrobenzene d5/p Terphenyl d14/2-Fluorobiphenyl 1,4 Dichlorobenzene d4/Naphthalene d8/Acenaphthene d10/Phenanthrene d10/Chrysene d12/Perylene d12 x no

14 Surrogates: Nitrobenzene-d5; 2-Fluorobiphenyl; Terphenyl-d14 Naphthalene-d8; Acenaphthene-d10; Phenanthrene-d10; Chrysene-d12; Perylene-d12 no

15 Surr IS x no

16 2-Fluorobiphenyl, Nitrobenzene-d5, Terphenyl-d14 Acenaphthene-d10, Chrysene-d12, Naphthalene-d8, Perylene-d12, Phenanthrene-d10 x yes

17 Nitrobenzene d5/p Terphenyl d14/2-Fluorobiphenyl 1,4 Dichlorobenzene d4/Naphthalene d8/Acenaphthene d10/Phenanthrene d10/Chrysene d12/Perylene d12 x no

18 Naphthalene-d8, Acenaphthene-d10, Phenanthrene-d10, Chrysene-d12, Pyrene-12 x yes

19 Nitrobenzene-d5 (S), 2-Fluorobiphenyl (S), and Terphenyl-d14 (S)

Naphthalene-d8 (IS), Acenaphthalene-d10 (IS), Phenanthrene-d10 (IS), Chrysene-d12 (IS), and Perylene-d12 

(IS) x no

20 PAH0235 (Surr) SVBIOM ISTD-00001 (IS) x no

21 naphthalene-d8, acenaphtene-d10, phenenthrene-d10, chrysene-d12, and perylene-d12 x fluorene-d10, benzo(a)pyrene-d12

22 Surrogates x IS x yes

23 d8Naph, d10Ace, d10Phen, d12Chry, d12B-a-P, d12Pery x hexamethylbenzene

24

d8-Napthalene, d8-1-Methylnapthalene, d8-Acenaphthylene, d10-Acenaphthene, d10-Fluorene, d10-

Phenanthrene, d10-Anthracene, d10-Fluoranthene, d10-Pyrene, d12-Benz(a)anthracene, d12-Chrysene, d12-

Benzo(b)fluoranthene, d12-Benzo(k)fluoranthene, d12-Benzo(e)pyrene, d12-Benzo(a)pyrene, d12-Perylene, 

d12-Benzo(g,h,i)perylene x d14-p-Terphenyl

25 Naphthalene-d8, Phenanthrene-d10, Fluoranthene-d10 x

26 surrogates only Internal standards x no
51

Qunatitation of Biomarkers

Biomarkers corrected for

Lab # IS/surrogate added prior to extraction Used? added prior to analysis Used? recovery? others?

1

naphthalene-d8;biphenyl-d10; acenaphthalene-d10; phenanthrene-d10; fluoranthene-d10; pyrene-d10; B[a]Ad12; B[a]P-d12; perylene-d12; B[ghi]P-d12;DB[a,h]A-d14 x

2 phenanthrene-d10 x no

3 Fluorene-d10, Benzo(a)pyrene-d12 x no

4 Naphthalene-d8, Acenaphthene-d10, Phenanthrene-d10, 5 alpha-Androstane, Chrysene-d12, Perylene-d12 Fluorene-d10, Pyrene-d10, Benzo(a)pyrene-d12 x yes

5 Nitrobenzene- d5; 2- Fluorobiphenyl; Terphenyl- d14 Naphthalene- d8; Acenapthene- d10; Chrysene- d12; Perylene- d12 x yes

6

7 NA NA

8 Nitrobenzene-d5, 2-Fluorobiphenyl, Terphenyl-d14 Naphthalene-d8, Acenaphthene-d10, Phenanthrene-d10, Chrysene-d10 x no

9 NA NA NA

10 NA NA

11 NA NA

12 5b(H)-Cholane Chrysene-d12 x yes

13 Nitrobenzene d5/p Terphenyl d14/2-Fluorobiphenyl 1,4 Dichlorobenzene d4/Naphthalene d8/Acenaphthene d10/Phenanthrene d10/Chrysene d12/Perylene d12 x no

14 Surrogates: Nitrobenzene-d5; 2-Fluorobiphenyl; Terphenyl-d14 Naphthalene-d8; Acenaphthene-d10; Phenanthrene-d10; Chrysene-d12; Perylene-d12 no

15 Surr IS x no

16 2-Fluorobiphenyl, Nitrobenzene-d5, Terphenyl-d14 Acenaphthene-d10, Chrysene-d12, Naphthalene-d8, Perylene-d12, Phenanthrene-d10 x yes

17 Nitrobenzene d5/p Terphenyl d14/2-Fluorobiphenyl 1,4 Dichlorobenzene d4/Naphthalene d8/Acenaphthene d10/Phenanthrene d10/Chrysene d12/Perylene d12 x no

18

19 Nitrobenzene-d5 (S), 2-Fluorobiphenyl (S), and Terphenyl-d14 (S)

Naphthalene-d8 (IS), Acenaphthalene-d10 (IS), Phenanthrene-d10 (IS), Chrysene-d12 (IS), and Perylene-d12 

(IS) x no

20 PAH0235 (Surr) SVBIOM ISTD-00001 (IS) x no

21 NA NA

22 Surrogates x IS x yes

23 d42Eicosane x dodecylcyclohexane

24 NA NA

25 n-triacontane-d62 x

26
52

PAHs - IS/surrogate used for quantitation

Lab 1 Lab 2 Lab 3 Lab 4 Lab 5 Lab 6

naphthalene naphthalene-d8 naphthalene-d8 Fluorene-d10

Fluorene-d10 / 

Naphthalene-d8 Napthalene- d8 naphthalene-d8

biphenyl biphenyl-d10 Fluorene-d10

Fluorene-d10 / 

Acenaphthene-d10 Acenapthene- d10 naphthalene-d8

acenaphthene acenaphthene-d10 acenaphthene-d10 Fluorene-d10

Fluorene-d10 / 

Acenaphthene-d10 Acenapthene- d10 acenaphthene-d10

acenaphthylene acenaphthene-d10 acenaphthene-d10 Fluorene-d10

Fluorene-d10 / 

Acenaphthene-d10 Acenapthene- d10 acenaphthylene-d8

fluorene phenanthrene-d10 acenaphthene-d10 Fluorene-d10

Fluorene-d10 / 

Acenaphthene-d10 Acenapthene- d10 fluorene-d10

phenanthrene phenanthrene-d10 phenanthrene-d10 Fluorene-d10

Pyrene-d10 / 

Phenanthrene-d10 Phananthrene- d10 phenanthrene-d10

anthracene phenanthrene-d10 phenanthrene-d10 Fluorene-d10

Pyrene-d10 / 

Phenanthrene-d10 Phananthrene- d10 anthracene-d10

fluoranthene fluoranathene-d10 phenanthrene-d10 Fluorene-d10

Pyrene-d10 / Chrysened12 Phananthrene- d10 fluoranthene-d10

pyrene pyrene-d10 chrysene-d12 Benzo(a)pyrene-d12

Pyrene-d10 / Chrysened12 Chyrsene- 12 pyrene-d10

benzo[b]fluorene NA Benzo(a)pyrene-d12

Pyrene-d10 / Chrysened12 NA

benz[a]anthracene B[a]A-d12 chrysene-d12 Benzo(a)pyrene-d12

Pyrene-d10 / Chrysened12 Chyrsene- 12 benz[a]anthracene-d12

chrysene B[a]A-d12 chrysene-d12 Benzo(a)pyrene-d12

Pyrene-d10 / Chrysened12 Chyrsene- 12 chrysene-d12

triphenylene B[a]A-d12

Pyrene-d10 / Chrysened12 NA

benzo[b ]fluoranthene B[a]P-d12 perylene-d12 Benzo(a)pyrene-d12

Benzo(a)pyrene-d12 / 

Chrysene-d12 Chyrsene- 12

benzo[b ]fluoranthened12

benzo[j ]fluoranthene B[a]P-d12

Benzo(a)pyrene-d12 / 

Chrysene-d12 NA

benzo[k ]fluoranthene B[a]P-d12 perylene-d12 Benzo(a)pyrene-d12

Benzo(a)pyrene-d12 / 

Chrysene-d12 Chyrsene- 12

benzo[k ]fluoranthened12

benzo[a]fluoranthene B[a]P-d12

Benzo(a)pyrene-d12 / 

Chrysene-d12 NA

benzo[e]pyrene B[a]P-d12 Benzo(a)pyrene-d12

Benzo(a)pyrene-d12 / 

Chrysene-d12 Chyrsene- 12

benzo[k ]fluoranthened12

benzo[a]pyrene B[a]P-d12 perylene-d12 Benzo(a)pyrene-d12

Benzo(a)pyrene-d12 / 

Chrysene-d12 Chyrsene- 12 benzo[a]pyrene-d12

perylene perylene-d12 Benzo(a)pyrene-d12

Benzo(a)pyrene-d12 / 

Chrysene-d12 Chyrsene- 12 benzo[a]pyrene-d12

indeno[1,2,3-cd]pyrene B[ghi]P-d12 perylene-d12 Benzo(a)pyrene-d12

Benzo(a)pyrene-d12 / 

Chrysene-d12 Chyrsene- 12

indeno[1,2,3-cd]pyrened12

benzo[ghi]perylene B[ghi]P-d12 perylene-d12 Benzo(a)pyrene-d12

Benzo(a)pyrene-d12 / 

Chrysene-d12 Chyrsene- 12 benzo[ghi]perylene-d12

dibenz[a,h]anthracene DB[a,h]A-d14 perylene-d12 Benzo(a)pyrene-d12

Benzo(a)pyrene-d12 / 

Chrysene-d12 Chyrsene- 12

dibenz[a,h]anthracened14

cis/trans-decalin NA Fluorene-d10

Fluorene-d10 / 

Naphthalene-d8 NA

dibenzofuran NA acenaphthene-d10 Fluorene-d10

Fluorene-d10 / 

Acenaphthene-d10 NA acenaphthylene-d8

retene NA

Pyrene-d10 / Chrysened12 NA

benzothiophene NA Fluorene-d10

Fluorene-d10 / 

Naphthalene-d8 NA

dibenzothiophene fluoranathene-d10 Fluorene-d10

Pyrene-d10 / 

Phenanthrene-d10 Phananthrene- d10 fluorene-d10

naphthobenzothiophene NA Benzo(a)pyrene-d12

Pyrene-d10 / Chrysened12 NA
53

PAHs - IS/surrogate used for quantitation

Lab 7 Lab 8 Lab 9 Lab 10 Lab 11 Lab 12

naphthalene d8-Naphthalene Naphthalene-d8 NPH-d8

Fluorened10/Phenanthrene-d10 2-fluorobiphenylFluorened10/Naphthalene-d8

biphenyl d10-Acenaphthene Naphthalene-d8 ACE-d10

Fluorened10/Phenanthrene-d10 2-fluorobiphenylFluorened10/Acenaphthene-d10

acenaphthene d10-Acenaphthene Acenaphthene-d10 ACE-d10

Fluorened10/Phenanthrene-d10 2-fluorobiphenylFluorened10/Acenaphthene-d10

acenaphthylene d10-Acenaphthene Acenaphthene-d10 ACE-d10

Fluorened10/Phenanthrene-d10 2-fluorobiphenylFluorened10/Acenaphthene-d10

fluorene d10-Acenaphthene Acenaphthene-d10 ACE-d10

Fluorened10/Phenanthrene-d10 2-fluorobiphenylFluorened10/Acenaphthene-d10

phenanthrene d10-Phenanthrene Phenanthrene-d10 ACE-d10

Pyrene-d10/Phenanthrened10 2-fluorobiphenylFluorened10/Phenanthrene-d10

anthracene d10-Phenanthrene Phenanthrene-d10 ACE-d10

Pyrene-d10/Phenanthrened10 2-fluorobiphenylFluorened10/Phenanthrene-d10

fluoranthene d10-Phenanthrene Phenanthrene-d10 BaP-d12

Pyrene-d10/Phenanthrened10 p-terphenyl d14Fluorened10/Phenanthrene-d10

pyrene d10-Phenanthrene Chrysene-d12 BaP-d12

Pyrene-d10/Phenanthrened10 p-terphenyl d14Fluorened10/Phenanthrene-d10

benzo[b]fluorene NA

Pyrene-d10/Phenanthrened10

benz[a]anthracene d12-Chrysene Chrysene-d12 BaP-d12

Pyrene-d10/Phenanthrened10 chrysene-d12Chrysened12/Phenanthrene-d10

chrysene d12-Chrysene Chrysene-d12

Pyrene-d10/Phenanthrened10 chrysene-d12Chrysened12/Phenanthrene-d10

triphenylene NA

Pyrene-d10/Phenanthrened10

benzo[b ]fluoranthene d12-Chrysene Chrysene-d12 BaP-d12

Benzo(a)pyrened12/Phenanthrene-d10 chrysene-d12Chrysened12/Benzo[a]pyrene-d12

benzo[j ]fluoranthene NA

Benzo(a)pyrened12/Phenanthrene-d10 chrysene-d12

benzo[k ]fluoranthene d12-Chrysene Chrysene-d12

Benzo(a)pyrened12/Phenanthrene-d10 chrysene-d12Chrysened12/Benzo[a]pyrene-d12

benzo[a]fluoranthene NA

Benzo(a)pyrened12/Phenanthrene-d10

benzo[e]pyrene d12-Chrysene Chrysene-d12 BaP-d12

Benzo(a)pyrened12/Phenanthrene-d10 chrysene-d12Chrysened12/Benzo[a]pyrene-d12

benzo[a]pyrene d12-Chrysene Chrysene-d12 BaP-d12

Benzo(a)pyrened12/Phenanthrene-d10 chrysene-d12Chrysened12/Benzo[a]pyrene-d12

perylene d12-Perylene Chrysene-d12 BaP-d12

Benzo(a)pyrened12/Phenanthrene-d10 chrysene-d12Chrysened12/Benzo[a]pyrene-d12

indeno[1,2,3-cd]pyrene d12-Chrysene Chrysene-d12 BaP-d12

Benzo(a)pyrened12/Phenanthrene-d10dibenzo(a,h)anthracene 

d14

Chrysened12/Benzo[a]pyrene-d12

benzo[ghi]perylene d12-Chrysene Chrysene-d12 BaP-d12

Benzo(a)pyrened12/Phenanthrene-d10dibenzo(a,h)anthracene 

d14

Chrysened12/Benzo[a]pyrene-d12

dibenz[a,h]anthracene d12-Chrysene Chrysene-d12

Benzo(a)pyrened12/Phenanthrene-d10dibenzo(a,h)anthracene 

d14

Chrysened12/Benzo[a]pyrene-d12

cis/trans-decalin NA

Fluorened10/Phenanthrene-d10Fluorened10/Naphthalene-d8

dibenzofuran Acenaphthene-d10

Fluorened10/Phenanthrene-d10Fluorened10/Acenaphthene-d10

retene NA ACE-d10

Pyrene-d10/Phenanthrened10 p-terphenyl d14Fluorened10/Phenanthrene-d10

benzothiophene NA

Fluorened10/Phenanthrene-d10Fluorened10/Acenaphthene-d10

dibenzothiophene d10-Phenanthrene Acenaphthene-d10 ACE-d10

Pyrene-d10/Phenanthrened10 p-terphenyl d14Fluorened10/Phenanthrene-d10

naphthobenzothiophene NA

Pyrene-d10/Phenanthrened10Fluorened10/Phenanthrene-d10
54

PAHs - IS/surrogate used for quantitation

Lab 13 Lab 14 Lab 15 Lab 16 Lab 17 Lab 18

naphthalene Naphthalene D8 Naphthalene-d8 Naphthalene-d8

Naphthalene-d8/Fluorened10

biphenyl Naphthalene D8 Acenaphthene-d10 Naphthalene-d8

Naphthalene-d8/Fluorened10

acenaphthene Acenaphthene D10 Acenaphthene-d10 Acenaphthene-d10

Acenaphthened10/Fluorene-d10

acenaphthylene Naphthalene D8 Acenaphthene-d10 Acenaphthene-d10

Acenaphthened10/Fluorene-d10

fluorene Acenaphthene D10 Acenaphthene-d10 Acenaphthene-d10

Acenaphthened10/Fluorene-d10

phenanthrene Phenanthrene D10 Phenanthrene-d10 Phenanthrene-d10

Phenanthrened10/Fluoranthene-d10

anthracene Phenanthrene D10 Phenanthrene-d10 Phenanthrene-d10

Phenanthrened10/Fluoranthene-d10

fluoranthene Phenanthrene D10 Phenanthrene-d10 Phenanthrene-d10

Phenanthrened10/Fluoranthene-d10

pyrene Phenanthrene D10 Chrysene-d12 Chrysene-d12

Chrysened12/Fluoranthene-d10

benzo[b]fluorene Phenanthrene D10 __________ Chrysene-d12

Chrysened12/Fluoranthene-d10

benz[a]anthracene Phenanthrene D10 Chrysene-d12 Chrysene-d12

Chrysene-d12/Terphenyld14

chrysene Chrysene D12 Chrysene-d12 Chrysene-d12

Chrysene-d12/Terphenyld14

triphenylene Chrysene D12 __________ 

Chrysene-d12/Terphenyld14

benzo[b ]fluoranthene Chrysene D12 Chrysene-d12 Chrysene-d12

Perylene-d12Terphenyld14

benzo[j ]fluoranthene Chrysene D12 __________ 

Perylene-d12Terphenyld14

benzo[k ]fluoranthene Chrysene D12 Chrysene-d12 Chrysene-d12

Perylene-d12Terphenyld14

benzo[a]fluoranthene __________ 

Perylene-d12Terphenyld14

benzo[e]pyrene Chrysene D12 Chrysene-d12 Chrysene-d12

Perylene-d12Terphenyld14

benzo[a]pyrene Chrysene D12 Chrysene-d12 Chrysene-d12

Perylene-d12Terphenyld14

perylene Perylene D12 Chrysene-d12 Chrysene-d12

Perylene-d12Terphenyld14

indeno[1,2,3-cd]pyrene Perylene D12 Chrysene-d12 Chrysene-d12

Perylene-d12Terphenyld14

benzo[ghi]perylene Perylene D12 Chrysene-d12 Chrysene-d12

Perylene-d12Terphenyld14

dibenz[a,h]anthracene Chrysene-d12 Chrysene-d12

Perylene-d12Terphenyld14

cis/trans-decalin 1,4-Dichlorobenzene D4 __________ 

Naphthalene-d8/Fluorened10

dibenzofuran Acenaphthene D10 Acenaphthene-d10 Acenaphthene-d10

Acenaphthened10/Fluorene-d10

retene Phenanthrene D10 __________ 

Chrysened12/Fluoranthene-d10

benzothiophene Naphthalene D8 __________ 

Naphthalene-d8/Fluorened10

dibenzothiophene Acenaphthene D10 Phenanthrene-d10 Acenaphthene-d10

Phenanthrened10/Fluoranthene-d10

naphthobenzothiophene Phenanthrene D10 __________ 

Chrysene-d12/Terphenyld14
55

PAHs - IS/surrogate used for quantitation

Lab 19 Lab 20 Lab 21 Lab 22 Lab 23 Lab 24

naphthalene Naphthalene-d8 Naphthalene-d8 naphthalene-d8 Acenaphthene-d10 d8-Naphthalene d8-Napthalene

biphenyl Acenaphthalene-d10 Naphthalene-d8 acenaphthene-d10 Acenaphthene-d10 d10-Acenaphthene d8-Napthalene

acenaphthene Acenaphthalene-d10 Acenaphthalene-d10 acenaphthene-d10 Acenaphthene-d10 d10-Acenaphthene d10Acenapthene

acenaphthylene Acenaphthalene-d10 Acenaphthalene-d10 acenaphthene-d10 Acenaphthene-d10 d10-Acenaphthene d8Acenapthylene

fluorene Acenaphthalene-d10 Acenaphthalene-d10 acenaphthene-d10 Acenaphthene-d10 d10-Acenaphthene d10Fluorene

phenanthrene Phenanthrene-d10 Phenanthrene-d10 phenenthrene-d10 Acenaphthene-d10 d10-Phenanthrene d10-Phenanthrene

anthracene Phenanthrene-d10 Phenanthrene-d10 phenenthrene-d10 Acenaphthene-d10 d10-Phenanthrene d10-Anthracene

fluoranthene Phenanthrene-d10 Phenanthrene-d10 phenenthrene-d10 Acenaphthene-d10 d10-Phenanthrene d10-Fluoranthene

pyrene Chrysene-d12 Chrysene-d12 phenenthrene-d10 Acenaphthene-d10 d10-Phenanthrene d10-Pyrene

benzo[b]fluorene Chrysene-d12 chrysene-d12 Acenaphthene-d10

d12-

Benzo(b)fluoranthene

benz[a]anthracene Chrysene-d12 Chrysene-d12 chrysene-d12 Chrysene-d12 d12-Chrysene d12-Benz(a)anthracene

chrysene Chrysene-d12 Chrysene-d12 chrysene-d12 Chrysene-d12 d12-Chrysene d12-Chrysene

triphenylene Chrysene-d12 NA Chrysene-d12 d12-Chrysene

benzo[b ]fluoranthene Perylene-d12 Chrysene-d12 chrysene-d12 Chrysene-d12 d12-Benzo-a-pyrene

d12-

Benzo(b)fluoranthene

benzo[j ]fluoranthene Perylene-d12 NA Chrysene-d12

d12-

Benzo(k)fluoranthene

benzo[k ]fluoranthene Perylene-d12 Chrysene-d12 chrysene-d12 Chrysene-d12 d12-Benzo-a-pyrene

d12-

Benzo(k)fluoranthene

benzo[a]fluoranthene Perylene-d12 NA Chrysene-d12 d12-Benzo(e)pyrene

benzo[e]pyrene Perylene-d12 Chrysene-d12 chrysene-d12 Chrysene-d12 d12-Benzo-a-pyrene d12-Benzo(e)pyrene

benzo[a]pyrene Perylene-d12 Chrysene-d12 chrysene-d12 Chrysene-d12 d12-Benzo-a-pyrene d12benzo(a)pyrene

perylene Perylene-d12 Chrysene-d12 perylene-d12 Chrysene-d12 d12-Perylene d12perylene

indeno[1,2,3-cd]pyrene Perylene-d12 Chrysene-d12 chrysene-d12 Chrysene-d12 d12-Benzo-a-pyrene d12benzo(ghi)perylene

benzo[ghi]perylene Perylene-d12 Chrysene-d12 chrysene-d12 Chrysene-d12 d12-Benzo-a-pyrene d12benzo(ghi)perylene

dibenz[a,h]anthracene Perylene-d12 Chrysene-d12 chrysene-d12 Chrysene-d12 d12-Benzo-a-pyrene d12benzo(ghi)perylene

cis/trans-decalin Naphthalene-d8 NA Acenaphthene-d10 d8-Napthalene

dibenzofuran Acenaphthalene-d10 Acenaphthalene-d10 NA Acenaphthene-d10 d10Acenapthene

retene Chrysene-d12 NA Acenaphthene-d10 d10-Pyrene

benzothiophene Naphthalene-d8 NA Acenaphthene-d10 d8-Napthalene

dibenzothiophene Acenaphthalene-d10 Acenaphthalene-d10 phenenthrene-d10 Acenaphthene-d10 d10-Phenanthrene d10-Phenanthrene

naphthobenzothiophene Chrysene-d12 NA Acenaphthene-d10
56

PAHs - IS/surrogate used for quantitation

Lab 25 Lab 26

naphthalene napthalene-d8 Naphthalene-D8

biphenyl napthalene-d8 Naphthalene-D8

acenaphthene napthalene-d8 Acenapthene-D10

acenaphthylene napthalene-d8 Acenapthene-D10

fluorene napthalene-d8 Acenapthene-D10

phenanthrene phenanthrene-d10 Phenanthrene-D10

anthracene phenanthrene-d10 Phenanthrene-D10

fluoranthene fluoranthene-d10 Phenanthrene-D10

pyrene pyrene-d10 Chrysene-D12

benzo[b]fluorene Perylene-D12

benz[a]anthracene benz[a]anthracene-d12 Chrysene-D12

chrysene benz[a]anthracene-d12 Chrysene-D12

triphenylene benz[a]anthracene-d12

benzo[b ]fluoranthene benzo[a]pyrene-d12 Perylene-D12

benzo[j ]fluoranthene benzo[a]pyrene-d12

benzo[k ]fluoranthene benzo[a]pyrene-d12 Perylene-D12

benzo[a]fluoranthene benzo[a]pyrene-d12 Perylene-D12

benzo[e]pyrene benzo[a]pyrene-d12 Perylene-D12

benzo[a]pyrene benzo[a]pyrene-d12 Perylene-D12

perylene perylene-d12 Perylene-D12

indeno[1,2,3-cd]pyrene benzo[ghi]perylene-d12 Perylene-D12

benzo[ghi]perylene perylene-d12 Perylene-D12

dibenz[a,h]anthracene

dibenz[a,h]anthracened14 Perylene-D12

cis/trans-decalin Naphthalene-D8

dibenzofuran Acenapthene-D10

retene fluoranthene-d10 Chrysene-D12

benzothiophene Naphthalene-D8

dibenzothiophene phenanthrene-d10 Phenanthrene-D10

naphthobenzothiophene Chrysene-D12
57

Alkylated PAHs - IS/surrogate used for quantitation

Lab 1 Lab 2 Lab 3 Lab 4 Lab 5 Lab 6

1-methylnaphthalene naphthalene-d8 Fluorene-d10

Fluorene-d10 / 

Naphthalene-d8 Napthalene- d8 naphthalene-d8

2-methylnaphthalene naphthalene-d8 naphthalene-d8 Fluorene-d10

Fluorene-d10 / 

Naphthalene-d8 Napthalene- d8 naphthalene-d8

2,6-dimethylnaphthalene naphthalene-d8 Fluorene-d10

Fluorene-d10 / 

Acenaphthene-d10 NA naphthalene-d8

1,6,7-trimethylnaphthalene naphthalene-d8 Fluorene-d10 NA acenaphthylene-d8

1-methylphenanthrene phenanthrene-d10 Fluorene-d10 Pyrene-d10 / Chrysene-d12 NA anthracene-d10

C1-decalins NA

Fluorene-d10 / 

Naphthalene-d8 NA

C2-decalins NA

Fluorene-d10 / 

Naphthalene-d8 NA

C3-decalins NA

Fluorene-d10 / 

Naphthalene-d8 NA

C4-decalins NA

Fluorene-d10 / 

Naphthalene-d8 NA

C1-naphthalenes naphthalene-d8 Fluorene-d10

Fluorene-d10 / 

Naphthalene-d8 Napthalene- d8

C2-naphthalenes naphthalene-d8 Fluorene-d10

Fluorene-d10 / 

Naphthalene-d8 Napthalene- d8

C3-naphthalenes naphthalene-d8 Fluorene-d10

Fluorene-d10 / 

Naphthalene-d8 Napthalene- d8

C4-naphthalenes naphthalene-d8 Fluorene-d10

Fluorene-d10 / 

Naphthalene-d8 Napthalene- d8

C1-benzothiophenes NA

Fluorene-d10 / 

Naphthalene-d8 NA

C2-benzothiophenes NA

Fluorene-d10 / 

Naphthalene-d8 NA

C3-benzothiophenes NA

Fluorene-d10 / 

Naphthalene-d8 NA

C4-benzothiophenes NA

Fluorene-d10 / 

Naphthalene-d8 NA

C1-fluorenes phenanthrene-d10 Fluorene-d10

Fluorene-d10 / 

Acenaphthene-d10 Acenapthene- d10

C2-fluorenes phenanthrene-d10 Fluorene-d10

Fluorene-d10 / 

Phenanthrene-d10 Acenapthene- d10

C3-fluorenes phenanthrene-d10 Fluorene-d10

Fluorene-d10 / 

Phenanthrene-d10 Acenapthene- d10

C1-phenanthrenes/anthracenes phenanthrene-d10 Fluorene-d10

Pyrene-d10 / Phenanthrened10 Phananthrene- d10

C2-phenanthrenes/anthracenes phenanthrene-d10 Fluorene-d10

Pyrene-d10 / Phenanthrened10 Phananthrene- d10

C3-phenanthrenes/anthracenes phenanthrene-d10 Fluorene-d10

Pyrene-d10 / Phenanthrened10 Phananthrene- d10

C4-phenanthrenes/anthracenes phenanthrene-d10 Fluorene-d10

Pyrene-d10 / Phenanthrened10 Phananthrene- d10

C1-dibenzothiophenes phenanthrene-d10 Fluorene-d10

Pyrene-d10 / Phenanthrened10 Phananthrene- d10

C2-dibenzothiophenes phenanthrene-d10 Fluorene-d10

Pyrene-d10 / Phenanthrened10 Phananthrene- d10

C3-dibenzothiophenes phenanthrene-d10 Fluorene-d10

Pyrene-d10 / Phenanthrened10 Phananthrene- d10

C4-dibenzothiophenes phenanthrene-d10 Fluorene-d10

Pyrene-d10 / Phenanthrened10 Phananthrene- d10

C1-fluoranthenes/pyrenes fluoranthene-d10 Benzo(a)pyrene-d12 Pyrene-d10 / Chrysene-d12 Chyrsene- 12

C2-fluoranthenes/pyrenes fluoranthene-d10 Benzo(a)pyrene-d12 Pyrene-d10 / Chrysene-d12 Chyrsene- 12

C3-fluoranthenes/pyrenes fluoranthene-d10 Benzo(a)pyrene-d12 Pyrene-d10 / Chrysene-d12 Chyrsene- 12

C4-fluoranthenes/pyrenes fluoranthene-d10 Pyrene-d10 / Chrysene-d12 NA

C1-naphthobenzothiophenes NA Pyrene-d10 / Chrysene-d12 NA

C2-naphthobenzothiophenes NA Pyrene-d10 / Chrysene-d12 NA

C3-naphthobenzothiophenes NA Pyrene-d10 / Chrysene-d12 NA

C4-naphthobenzothiophenes NA Pyrene-d10 / Chrysene-d12 NA

C1-chrysenes B[a]A-d12 Benzo(a)pyrene-d12 Pyrene-d10 / Chrysene-d12 Chyrsene- 12

C2-chrysenes B[a]A-d12 Benzo(a)pyrene-d12 Pyrene-d10 / Chrysene-d12 Chyrsene- 12

C3-chrysenes B[a]A-d12 Benzo(a)pyrene-d12 Pyrene-d10 / Chrysene-d12 Chyrsene- 12

C4-chrysenes B[a]A-d12 Benzo(a)pyrene-d12 Pyrene-d10 / Chrysene-d12 NA
58

Alkylated PAHs - IS/surrogate used for quantitation

Lab 7 Lab 8 Lab 9 Lab 10 Lab 11 Lab 12

1-methylnaphthalene d8-Naphthalene Naphthalene-d8 NPH-d8

Fluorene-d10/Phenanthrened10 2-fluorobiphenylFluorene-d10/Naphthalened8

2-methylnaphthalene d8-Naphthalene Naphthalene-d8 NPH-d8

Fluorene-d10/Phenanthrened10 2-fluorobiphenylFluorene-d10/Naphthalened8

2,6-dimethylnaphthalene d10-Acenaphthene Naphthalene-d8 ACE-d10

Fluorene-d10/Phenanthrened10Fluorened10/Acenaphthene-d10

1,6,7-trimethylnaphthalene d10-Acenaphthene Acenaphthene-d10 ACE-d10

Fluorene-d10/Phenanthrened10Fluorened10/Acenaphthene-d10

1-methylphenanthrene d10-Phenanthrene Phenanthrene-d10 ACE-d10

Pyrene-d10/Phenanthrened10Fluorene-d10/Phenanthrened10

C1-decalins NA

Fluorene-d10/Phenanthrened10Fluorene-d10/Naphthalened8

C2-decalins NA

Fluorene-d10/Phenanthrened10Fluorene-d10/Naphthalened8

C3-decalins NA

Fluorene-d10/Phenanthrened10Fluorene-d10/Naphthalened8

C4-decalins NA

Fluorene-d10/Phenanthrened10Fluorene-d10/Naphthalened8

C1-naphthalenes Naphthalene-d8 ACE-d10

Fluorene-d10/Phenanthrened10Fluorene-d10/Naphthalened8

C2-naphthalenes d8-Naphthalene Naphthalene-d8 ACE-d10

Fluorene-d10/Phenanthrened10 2-fluorobiphenylFluorened10/Acenaphthene-d10

C3-naphthalenes d8-Naphthalene Naphthalene-d8 ACE-d10

Fluorene-d10/Phenanthrened10 2-fluorobiphenylFluorened10/Acenaphthene-d10

C4-naphthalenes d8-Naphthalene Naphthalene-d8 ACE-d10

Fluorene-d10/Phenanthrened10 2-fluorobiphenylFluorened10/Acenaphthene-d10

C1-benzothiophenes NA

Fluorene-d10/Phenanthrened10Fluorened10/Acenaphthene-d10

C2-benzothiophenes NA

Fluorene-d10/Phenanthrened10Fluorened10/Acenaphthene-d10

C3-benzothiophenes NA

Fluorene-d10/Phenanthrened10Fluorened10/Acenaphthene-d10

C4-benzothiophenes NA

Fluorene-d10/Phenanthrened10Fluorened10/Acenaphthene-d10

C1-fluorenes d10-Acenaphthene Acenaphthene-d10 ACE-d10

Fluorene-d10/Phenanthrened10 2-fluorobiphenylFluorened10/Acenaphthene-d10

C2-fluorenes d10-Acenaphthene Acenaphthene-d10 ACE-d10

Fluorene-d10/Phenanthrened10 2-fluorobiphenylFluorened10/Acenaphthene-d10

C3-fluorenes d10-Acenaphthene Acenaphthene-d10 ACE-d10

Fluorene-d10/Phenanthrened10 2-fluorobiphenylFluorened10/Acenaphthene-d10

C1-phenanthrenes/anthracenes d10-Phenanthrene Phenanthrene-d10 ACE-d10

Pyrene-d10/Phenanthrened10 p-terphenyl d14Fluorene-d10/Phenanthrened10

C2-phenanthrenes/anthracenes d10-Phenanthrene Phenanthrene-d10 ACE-d10

Pyrene-d10/Phenanthrened10 p-terphenyl d14Fluorene-d10/Phenanthrened10

C3-phenanthrenes/anthracenes d10-Phenanthrene Phenanthrene-d10 ACE-d10

Pyrene-d10/Phenanthrened10 p-terphenyl d14Fluorene-d10/Phenanthrened10

C4-phenanthrenes/anthracenes d10-Phenanthrene Phenanthrene-d10 ACE-d10

Pyrene-d10/Phenanthrened10 p-terphenyl d14Fluorene-d10/Phenanthrened10

C1-dibenzothiophenes d10-Phenanthrene Acenaphthene-d10 ACE-d10

Pyrene-d10/Phenanthrened10 p-terphenyl d14Fluorene-d10/Phenanthrened10

C2-dibenzothiophenes d10-Phenanthrene Acenaphthene-d10 ACE-d10

Pyrene-d10/Phenanthrened10 p-terphenyl d14Fluorene-d10/Phenanthrened10

C3-dibenzothiophenes d10-Phenanthrene Acenaphthene-d10 ACE-d10

Pyrene-d10/Phenanthrened10 p-terphenyl d14Fluorene-d10/Phenanthrened10

C4-dibenzothiophenes Acenaphthene-d10 ACE-d10

Pyrene-d10/Phenanthrened10 p-terphenyl d14Fluorene-d10/Phenanthrened10

C1-fluoranthenes/pyrenes d10-Phenanthrene Chrysene-d12 BaP-d12

Pyrene-d10/Phenanthrened10 p-terphenyl d14Fluorene-d10/Phenanthrened10

C2-fluoranthenes/pyrenes d10-Phenanthrene Chrysene-d12 BaP-d12

Pyrene-d10/Phenanthrened10 p-terphenyl d14Fluorene-d10/Phenanthrened10

C3-fluoranthenes/pyrenes d10-Phenanthrene Chrysene-d12 BaP-d12

Pyrene-d10/Phenanthrened10 p-terphenyl d14Fluorene-d10/Phenanthrened10

C4-fluoranthenes/pyrenes NA BaP-d12

Pyrene-d10/Phenanthrened10 p-terphenyl d14Fluorene-d10/Phenanthrened10

C1-naphthobenzothiophenes NA ACE-d10

Pyrene-d10/Phenanthrened10Fluorene-d10/Phenanthrened10

C2-naphthobenzothiophenes NA ACE-d10

Pyrene-d10/Phenanthrened10Fluorene-d10/Phenanthrened10

C3-naphthobenzothiophenes NA ACE-d10

Pyrene-d10/Phenanthrened10Fluorene-d10/Phenanthrened10

C4-naphthobenzothiophenes NA ACE-d10

Pyrene-d10/Phenanthrened10Fluorene-d10/Phenanthrened10

C1-chrysenes d12-Chrysene Chrysene-d12 BaP-d12

Pyrene-d10/Phenanthrened10Chrysened12/Phenanthrene-d10

C2-chrysenes d12-Chrysene Chrysene-d12 BaP-d12

Pyrene-d10/Phenanthrened10Chrysened12/Phenanthrene-d10

C3-chrysenes d12-Chrysene Chrysene-d12 BaP-d12

Pyrene-d10/Phenanthrened10Chrysened12/Phenanthrene-d10

C4-chrysenes d12-Chrysene Chrysene-d12 BaP-d12

Pyrene-d10/Phenanthrened10Chrysened12/Phenanthrene-d10
59

Alkylated PAHs - IS/surrogate used for quantitation

Lab 13 Lab 14 Lab 15 Lab 16 Lab 17 Lab 18

1-methylnaphthalene Naphthalene D8 Naphthalene-d8 Naphthalene-d8

Naphthalene-d8/Fluorened10

2-methylnaphthalene Naphthalene D8 Acenaphthene-d10 Naphthalene-d8

Naphthalene-d8/Fluorened10

2,6-dimethylnaphthalene Naphthalene D8 Acenaphthene-d10 Naphthalene-d8

Naphthalene-d8/Fluorened10

1,6,7-trimethylnaphthalene Acenaphthene-d10

Naphthalene-d8/Fluorened10

1-methylphenanthrene Phenanthrene D10 Phenanthrene-d10 Phenanthrene-d10

Phenanthrened10/Fluoranthene-d10

C1-decalins 1,4-Dichlorobenzene D4 __________ 

Naphthalene-d8/Fluorened10

C2-decalins __________ 

Naphthalene-d8/Fluorened10

C3-decalins __________ 

Naphthalene-d8/Fluorened10

C4-decalins __________ 

Naphthalene-d8/Fluorened10

C1-naphthalenes Naphthalene-d8 Naphthalene-d8

Naphthalene-d8/Fluorened10

C2-naphthalenes Naphthalene D8 Acenaphthene-d10 Naphthalene-d8

Naphthalene-d8/Fluorened10

C3-naphthalenes Naphthalene D8 Acenaphthene-d10 Naphthalene-d8

Naphthalene-d8/Fluorened10

C4-naphthalenes Naphthalene D8 Phenanthrene-d10 Naphthalene-d8

Naphthalene-d8/Fluorened10

C1-benzothiophenes Naphthalene D8 __________ 

Naphthalene-d8/Fluorened10

C2-benzothiophenes Naphthalene D8 __________ 

Naphthalene-d8/Fluorened10

C3-benzothiophenes Naphthalene D8 __________ 

Naphthalene-d8/Fluorened10

C4-benzothiophenes __________ 

Naphthalene-d8/Fluorened10

C1-fluorenes Acenaphthene D10 Phenanthrene-d10 Acenaphthene-d10

Acenaphthened10/Fluorene-d10

C2-fluorenes Phenanthrene-d10 Acenaphthene-d10

Acenaphthened10/Fluorene-d10

C3-fluorenes Acenaphthene D10 Phenanthrene-d10 Acenaphthene-d10

Acenaphthened10/Fluorene-d10

C1-phenanthrenes/anthracenes Phenanthrene D10 Phenanthrene-d10 Phenanthrene-d10

Phenanthrened10/Fluoranthene-d10

C2-phenanthrenes/anthracenes Phenanthrene D10 Phenanthrene-d10 Phenanthrene-d10

Phenanthrened10/Fluoranthene-d10

C3-phenanthrenes/anthracenes Phenanthrene D10 Chrysene-d12 Phenanthrene-d10

Phenanthrened10/Fluoranthene-d10

C4-phenanthrenes/anthracenes Phenanthrene D10 Chrysene-d12 Phenanthrene-d10

Phenanthrened10/Fluoranthene-d10

C1-dibenzothiophenes Phenanthrene D10 Phenanthrene-d10 Phenanthrene-d10

Phenanthrened10/Fluoranthene-d10

C2-dibenzothiophenes Phenanthrene D10 Phenanthrene-d10 Phenanthrene-d10

Phenanthrened10/Fluoranthene-d10

C3-dibenzothiophenes Phenanthrene D10 Phenanthrene-d10 Phenanthrene-d10

Phenanthrened10/Fluoranthene-d10

C4-dibenzothiophenes Phenanthrene D10 Chrysene-d12 Phenanthrene-d10

Phenanthrened10/Fluoranthene-d10

C1-fluoranthenes/pyrenes Phenanthrene D10 Chrysene-d12 Chrysene-d12

Phenanthrened10/Fluoranthene-d10

C2-fluoranthenes/pyrenes Chrysene-d12 Chrysene-d12

Phenanthrened10/Fluoranthene-d10

C3-fluoranthenes/pyrenes Phenanthrene D10 Chrysene-d12 Chrysene-d12

Phenanthrened10/Fluoranthene-d10

C4-fluoranthenes/pyrenes Phenanthrene D10 __________ 

Phenanthrened10/Fluoranthene-d10

C1-naphthobenzothiophenes __________ 

Chrysene-d12/Terphenyld14

C2-naphthobenzothiophenes __________ 

Chrysene-d12/Terphenyld14

C3-naphthobenzothiophenes __________ 

Chrysene-d12/Terphenyld14

C4-naphthobenzothiophenes __________ 

Chrysene-d12/Terphenyld14

C1-chrysenes Chrysene D12 Chrysene-d12 Chrysene-d12

Chrysene-d12/Terphenyld14

C2-chrysenes Chrysene D12 Chrysene-d12 Chrysene-d12

Chrysene-d12/Terphenyld14

C3-chrysenes Chrysene-d12 Chrysene-d12

Chrysene-d12/Terphenyld14

C4-chrysenes Chrysene D12 Chrysene-d12 Chrysene-d12

Chrysene-d12/Terphenyld14
60

Alkylated PAHs - IS/surrogate used for quantitation

Lab 19 Lab 20 Lab 21 Lab 22 Lab 23 Lab 24

1-methylnaphthalene Naphthalene-d8 Naphthalene-d8 naphthalene-d8 Acenaphthene-d10 d8-Naphthalene d8-Napthalene

2-methylnaphthalene Naphthalene-d8 Naphthalene-d8 naphthalene-d8 Acenaphthene-d10 d8-Naphthalene d8-Napthalene

2,6-dimethylnaphthalene Acenaphthalene-d10 Naphthalene-d8 naphthalene-d8 Acenaphthene-d10 d10-Acenaphthene d8-1-MeNaphthalene

1,6,7-trimethylnaphthalene Acenaphthalene-d10 Phenanthrene-d10 naphthalene-d8 Acenaphthene-d10 d8-1-MeNaphthalene

1-methylphenanthrene Phenanthrene-d10 Phenanthrene-d10 phenenthrene-d10 Acenaphthene-d10 d10-Phenanthrene d10-Pyrene

C1-decalins Naphthalene-d8 NA Acenaphthene-d10 d8-Napthalene

C2-decalins Naphthalene-d8 NA Acenaphthene-d10 d8-Napthalene

C3-decalins Naphthalene-d8 NA Acenaphthene-d10 d8-Napthalene

C4-decalins Naphthalene-d8 NA Acenaphthene-d10 d8-Napthalene

C1-naphthalenes Naphthalene-d8 Naphthalene-d8 naphthalene-d8 Acenaphthene-d10 d8-Naphthalene d8-1-MeNaphthalene

C2-naphthalenes Naphthalene-d8 Naphthalene-d8 naphthalene-d8 Acenaphthene-d10 d10-Acenaphthene d8-1-MeNaphthalene

C3-naphthalenes Naphthalene-d8 Naphthalene-d8 naphthalene-d8 Acenaphthene-d10 d10-Acenaphthene d8-1-MeNaphthalene

C4-naphthalenes Naphthalene-d8 Naphthalene-d8 naphthalene-d8 Acenaphthene-d10 d10-Acenaphthene d8-1-MeNaphthalene

C1-benzothiophenes Naphthalene-d8 NA Acenaphthene-d10 d8-1-MeNaphthalene

C2-benzothiophenes Naphthalene-d8 NA Acenaphthene-d10 d8-1-MeNaphthalene

C3-benzothiophenes Naphthalene-d8 NA Acenaphthene-d10 d8-1-MeNaphthalene

C4-benzothiophenes Naphthalene-d8 NA Acenaphthene-d10 d8-1-MeNaphthalene

C1-fluorenes Acenaphthalene-d10 Acenaphthalene-d10 acenaphthene-d10 Acenaphthene-d10 d10-Acenaphthene d10Fluorene

C2-fluorenes Acenaphthalene-d10 Acenaphthalene-d10 acenaphthene-d10 Acenaphthene-d10 d10-Acenaphthene d10Fluorene

C3-fluorenes Acenaphthalene-d10 Acenaphthalene-d10 acenaphthene-d10 Acenaphthene-d10 d10-Acenaphthene d10Fluorene

C1-phenanthrenes/anthracenes Phenanthrene-d10 Phenanthrene-d10 phenenthrene-d10 Acenaphthene-d10 d10-Phenanthrene d10-Anthracene

C2-phenanthrenes/anthracenes Phenanthrene-d10 Phenanthrene-d10 phenenthrene-d10 Acenaphthene-d10 d10-Phenanthrene d10-Anthracene

C3-phenanthrenes/anthracenes Phenanthrene-d10 Phenanthrene-d10 phenenthrene-d10 Acenaphthene-d10 d10-Phenanthrene d10-Anthracene

C4-phenanthrenes/anthracenes Phenanthrene-d10 Phenanthrene-d10 phenenthrene-d10 Acenaphthene-d10 d10-Phenanthrene d10-Anthracene

C1-dibenzothiophenes Acenaphthalene-d10 Chrysene-d12 phenenthrene-d10 Acenaphthene-d10 d10-Phenanthrene d8-dibenzothiophene

C2-dibenzothiophenes Acenaphthalene-d10 Chrysene-d12 phenenthrene-d10 Acenaphthene-d10 d10-Phenanthrene d8-dibenzothiophene

C3-dibenzothiophenes Acenaphthalene-d10 Chrysene-d12 phenenthrene-d10 Acenaphthene-d10 d10-Phenanthrene d8-dibenzothiophene

C4-dibenzothiophenes Acenaphthalene-d10 Chrysene-d12 NA Acenaphthene-d10 d10-Phenanthrene d8-dibenzothiophene

C1-fluoranthenes/pyrenes Phenanthrene-d10 Chrysene-d12 phenenthrene-d10 Acenaphthene-d10 d10-Phenanthrene d10-Fluoranthene

C2-fluoranthenes/pyrenes Phenanthrene-d10 Chrysene-d12 NA Acenaphthene-d10 d10-Phenanthrene d10-Fluoranthene

C3-fluoranthenes/pyrenes Phenanthrene-d10 Chrysene-d12 NA Acenaphthene-d10 d10-Phenanthrene d10-Fluoranthene

C4-fluoranthenes/pyrenes Phenanthrene-d10 NA Acenaphthene-d10 d10-Phenanthrene d10-Fluoranthene

C1-naphthobenzothiophenes Chrysene-d12 NA Acenaphthene-d10

C2-naphthobenzothiophenes Chrysene-d12 NA Acenaphthene-d10

C3-naphthobenzothiophenes Chrysene-d12 NA Acenaphthene-d10

C4-naphthobenzothiophenes Chrysene-d12 NA Acenaphthene-d10

C1-chrysenes Chrysene-d12 Chrysene-d12 chrysene-d12 Chrysene-d12 d12-Chrysene d12-Chrysene

C2-chrysenes Chrysene-d12 Chrysene-d12 chrysene-d12 Chrysene-d12 d12-Chrysene d12-Chrysene

C3-chrysenes Chrysene-d12 Chrysene-d12 chrysene-d12 Chrysene-d12 d12-Chrysene d12-Chrysene

C4-chrysenes Chrysene-d12 Chrysene-d12 chrysene-d12 Chrysene-d12 d12-Chrysene d12-Chrysene
61

Alkylated PAHs - IS/surrogate used for quantitation

Lab 25 Lab 26

1-methylnaphthalene Naphthalene-D8

2-methylnaphthalene Naphthalene-D8

2,6-dimethylnaphthalene napthalene-d8 Naphthalene-D8

1,6,7-trimethylnaphthalene Naphthalene-D8

1-methylphenanthrene phenanthrene-d10 Phenanthrene-D10

C1-decalins Naphthalene-D8

C2-decalins Naphthalene-D8

C3-decalins Naphthalene-D8

C4-decalins Naphthalene-D8

C1-naphthalenes Naphthalene-D8

C2-naphthalenes Naphthalene-D8

C3-naphthalenes Naphthalene-D8

C4-naphthalenes Naphthalene-D8

C1-benzothiophenes Naphthalene-D8

C2-benzothiophenes Naphthalene-D8

C3-benzothiophenes Naphthalene-D8

C4-benzothiophenes Naphthalene-D8

C1-fluorenes Acenapthene-D10

C2-fluorenes Acenapthene-D10

C3-fluorenes Acenapthene-D10

C1-phenanthrenes/anthracenes Phenanthrene-D10

C2-phenanthrenes/anthracenes Phenanthrene-D10

C3-phenanthrenes/anthracenes Phenanthrene-D10

C4-phenanthrenes/anthracenes Phenanthrene-D10

C1-dibenzothiophenes Phenanthrene-D10

C2-dibenzothiophenes Phenanthrene-D10

C3-dibenzothiophenes Phenanthrene-D10

C4-dibenzothiophenes Phenanthrene-D10

C1-fluoranthenes/pyrenes Phenanthrene-D10

C2-fluoranthenes/pyrenes Phenanthrene-D10

C3-fluoranthenes/pyrenes Phenanthrene-D10

C4-fluoranthenes/pyrenes Phenanthrene-D10

C1-naphthobenzothiophenes Chrysene-D12

C2-naphthobenzothiophenes Chrysene-D12

C3-naphthobenzothiophenes Chrysene-D12

C4-naphthobenzothiophenes Chrysene-D12

C1-chrysenes Chrysene-D12

C2-chrysenes Chrysene-D12

C3-chrysenes Chrysene-D12

C4-chrysenes Chrysene-D12
62

Biomarkers - IS/surrogate used for quantitation

Lab 1 Lab 2 Lab 3 Lab 4 Lab 5 Lab 6 Lab 7 Lab 8 Lab 9 Lab 10 Lab 11 Lab 12 Lab 13

Carbazole NA Benzo(a)pyrene-d12

Pyrene-d10 / 

Phenanthrene-d10 NA NA NA

Chrysened12/5b(H)Cholane Chrysene D12

18a(H)-22,29,30-Trisnorneohopane NA Benzo(a)pyrene-d12 Chyrsene- 12 Chrysene-d12 NA

Chrysened12/5b(H)Cholane

17a(H)-22,29,30-Trisnorhopane B[a]A-d12 Benzo(a)pyrene-d12 Chyrsene- 12 Chrysene-d12 NA

Chrysened12/5b(H)Cholane Chrysene D12

17α(H),21β(H)-30-Norhopane B[a]A-d12 Benzo(a)pyrene-d12 Chyrsene- 12 Chrysene-d12 NA

Chrysened12/5b(H)Cholane

18a(H)-30-Norneohopane NA Benzo(a)pyrene-d12 Chyrsene- 12 Chrysene-d12 NA

Chrysened12/5b(H)Cholane

17a(H)-Diahopane NA Benzo(a)pyrene-d12 Chyrsene- 12 Chrysene-d12 NA

Chrysened12/5b(H)Cholane

17α(H),21β(H)-Hopane B[a]A-d12 Benzo(a)pyrene-d12

Benzo(a)pyrene-d12 / 

Chrysene-d12 Chyrsene- 12 Chrysene-d12 NA

Chrysened12/5b(H)Cholane Chrysene D12

17α(H),21β(H)-22R-Homohopane B[a]A-d12 Benzo(a)pyrene-d12 Chyrsene- 12 Chrysene-d12 NA

Chrysened12/5b(H)Cholane

17α(H),21β(H)-22S-Homohopane B[a]A-d12 Benzo(a)pyrene-d12 Chyrsene- 12 Chrysene-d12 NA

Chrysened12/5b(H)Cholane

13b(H)17a(H)-Diacholestane 20S NA Benzo(a)pyrene-d12 Chyrsene- 12 Chrysene-d12 NA

Chrysened12/5b(H)Cholane

5a(H),14a(H),17a(H)-Cholestane 20S NA Benzo(a)pyrene-d12 NA Chrysene-d12 NA

Chrysened12/5b(H)Cholane

5a(H),14a(H),17a(H)-Cholestane 20R B[a]A-d12 Benzo(a)pyrene-d12 Chyrsene- 12 Chrysene-d12 NA

Chrysened12/5b(H)Cholane Chrysene D12

5a(H),14a(H),17a(H)-24-Ethylcholestane 20S NA Benzo(a)pyrene-d12 Chyrsene- 12 Chrysene-d12 NA

Chrysened12/5b(H)Cholane

5a(H),14a(H),17a(H)-24-Ethylcholestane 20R B[a]A-d12 Benzo(a)pyrene-d12 Chyrsene- 12 Chrysene-d12 NA

Chrysened12/5b(H)Cholane Chrysene D12

5a(H),14b(H),17b(H)-Cholestane 20R B[a]A-d12 Benzo(a)pyrene-d12 Chyrsene- 12 Chrysene-d12 NA

Chrysened12/5b(H)Cholane

5a(H),14b(H),17b(H)-Cholestane 20S NA Benzo(a)pyrene-d12 Chyrsene- 12 Chrysene-d12 NA

Chrysened12/5b(H)Cholane

5a(H),14b(H),17b(H)-24-Ethylcholestane 20R B[a]A-d12 Benzo(a)pyrene-d12 Chyrsene- 12 Chrysene-d12 NA

Chrysened12/5b(H)Cholane

5a(H),14b(H),17b(H)-24-Ethylcholestane 20S NA Chyrsene- 12 Chrysene-d12 NA
63

Biomarkers - IS/surrogate used for quantitation

Lab 14 Lab 15 Lab 16 Lab 17 Lab 18 Lab 19 Lab 20 Lab 21 Lab 22 Lab 23 Lab 24 Lab 25 Lab 26

Carbazole Chrysene-d12

Phenanthrened10/Fluoranthene-d10 Phenanthrene-d10 phenenthrene-d10 Chrysene-d12

18a(H)-22,29,30-Trisnorneohopane Chrysene-d12 Chrysene-d12 NA Chrysene-d12 DCH

17a(H)-22,29,30-Trisnorhopane Chrysene-d12 Chrysene-d12 Chrysene-d12 NA Chrysene-d12 DCH n-triacontane-d62

17α(H),21β(H)-30-Norhopane Chrysene-d12 Chrysene-d12 Perylene-d12 Chrysene-d12 NA Chrysene-d12 DCH n-triacontane-d62

18a(H)-30-Norneohopane Chrysene-d12 Chrysene-d12 Chrysene-d12 NA Chrysene-d12 DCH

17a(H)-Diahopane Chrysene-d12 Chrysene-d12 Chrysene-d12 NA Chrysene-d12

17α(H),21β(H)-Hopane Chrysene-d12 Chrysene-d12

Chrysene-d12/Terphenyld14 Chrysene-d12 NA Chrysene-d12 DCH n-triacontane-d62

17α(H),21β(H)-22R-Homohopane Chrysene-d12 Chrysene-d12 Chrysene-d12 NA Chrysene-d12 DCH n-triacontane-d62

17α(H),21β(H)-22S-Homohopane Chrysene-d12 Chrysene-d12 Chrysene-d12 NA Chrysene-d12 DCH n-triacontane-d62

13b(H)17a(H)-Diacholestane 20S Chrysene-d12 Chrysene-d12 Chrysene-d12 NA Chrysene-d12

5a(H),14a(H),17a(H)-Cholestane 20S Chrysene-d12 Chrysene-d12 Chrysene-d12 NA Chrysene-d12 DCH

5a(H),14a(H),17a(H)-Cholestane 20R Chrysene-d12 Chrysene-d12 Chrysene-d12 NA Chrysene-d12 DCH n-triacontane-d62

5a(H),14a(H),17a(H)-24-Ethylcholestane 20S Chrysene-d12 Chrysene-d12 Chrysene-d12 NA Chrysene-d12 DCH

5a(H),14a(H),17a(H)-24-Ethylcholestane 20R Chrysene-d12 Chrysene-d12 Chrysene-d12 NA Chrysene-d12 DCH n-triacontane-d62

5a(H),14b(H),17b(H)-Cholestane 20R Chrysene-d12 Chrysene-d12 Chrysene-d12 NA Chrysene-d12 DCH n-triacontane-d62

5a(H),14b(H),17b(H)-Cholestane 20S Chrysene-d12 Chrysene-d12 Chrysene-d12 NA Chrysene-d12 DCH

5a(H),14b(H),17b(H)-24-Ethylcholestane 20R Chrysene-d12 Chrysene-d12 Chrysene-d12 NA Chrysene-d12 DCH n-triacontane-d62

5a(H),14b(H),17b(H)-24-Ethylcholestane 20S Chrysene-d12 Chrysene-d12 Chrysene-d12 NA Chrysene-d12 DCH
64

PAHs - Associated % recovery / acceptance ranges

Lab 1 Lab 2 Lab 3 Lab 4 Lab 5 Lab 6 Lab 7 Lab 8 Lab 9 Lab 10

naphthalene 50-200 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

biphenyl 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

acenaphthene 50-200 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

acenaphthylene 50-200 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

fluorene 50-200 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

phenanthrene 50-200 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

anthracene 50-200 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

fluoranthene 50-200 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

pyrene 50-200 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

benzo[b]fluorene 40%-120%

minus 50 to 

plus 100 NA 60-120

benz[a]anthracene 50-200 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

chrysene 50-200 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

triphenylene 40%-120%

minus 50 to 

plus 100 NA NA

benzo[b ]fluoranthene 50-200 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

benzo[j ]fluoranthene 40%-120%

minus 50 to 

plus 100 NA NA

benzo[k ]fluoranthene 50-200 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

benzo[a]fluoranthene 40%-120%

minus 50 to 

plus 100 NA 60-120

benzo[e]pyrene 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

benzo[a]pyrene 50-200 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

perylene 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

indeno[1,2,3-cd]pyrene 50-200 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

benzo[ghi]perylene 50-200 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

dibenz[a,h]anthracene 50-200 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

cis/trans-decalin 40%-120%

minus 50 to 

plus 100 NA 60-120

dibenzofuran 50-200 40%-120%

minus 50 to 

plus 100 60-140 60-120

retene 40%-120%

minus 50 to 

plus 100 NA 60-120

benzothiophene 40%-120%

minus 50 to 

plus 100 NA 60-120

dibenzothiophene 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

naphthobenzothiophene 40%-120%

minus 50 to 

plus 100 NA 60-120
65

PAHs - Associated % recovery / acceptance ranges

Lab 11 Lab 12 Lab 13 Lab 14 Lab 15 Lab 16 Lab 17 Lab 18 Lab 19 Lab 20

naphthalene 50-150 70 - 130% 70-130 60 - 140

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

biphenyl 50-150 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

acenaphthene 50-150 70 - 130% 70-130 60 - 140

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

acenaphthylene 50-150 70 - 130% 70-130 60 - 140

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

fluorene 50-150 70 - 130% 70-130 60 - 140

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

phenanthrene 50-150 70 - 130% 70-130 60 - 140

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

anthracene 50-150 70 - 130% 70-130 60 - 140

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

fluoranthene 50-150 70 - 130% 70-130 60 - 140

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

pyrene 50-150 70 - 130% 70-130 60 - 140

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

benzo[b]fluorene 50-150 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100%

benz[a]anthracene 50-150 70 - 130% 70-130 60 - 140

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

chrysene 50-150 70 - 130% 70-130 60 - 140

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

triphenylene 50-150 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100%

benzo[b ]fluoranthene 50-150 70 - 130% 70-130 60 - 140

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

benzo[j ]fluoranthene 50-150 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100%

benzo[k ]fluoranthene 50-150 70 - 130% 70-130 60 - 140

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

benzo[a]fluoranthene 50-150 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100%

benzo[e]pyrene 50-150 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

benzo[a]pyrene 50-150 70 - 130% 70-130 60 - 140

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

perylene 50-150 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

indeno[1,2,3-cd]pyrene 50-150 70 - 130% 70-130 60 - 140

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

benzo[ghi]perylene 50-150 70 - 130% 70-130 60 - 140

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

dibenz[a,h]anthracene 50-150 70 - 130% 70-130 60 - 140

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

cis/trans-decalin 50-150 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100%

dibenzofuran 50-150 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

retene 50-150 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100%

benzothiophene 50-150 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100%

dibenzothiophene 50-150 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

naphthobenzothiophene 50-150 70 - 130% 70-130 40-120

 -50% to + 

100%
66

PAHs - Associated % recovery / acceptance ranges

Lab 21 Lab 22 Lab 23 Lab 24 Lab 25 Lab 26

naphthalene 50-150 40-120 30-120

biphenyl 50-150 40-120 30-120

acenaphthene 50-150 40-120 30-120

acenaphthylene 50-150 40-120 30-120

fluorene 50-150 40-120 30-120

phenanthrene 50-150 40-120 30-120

anthracene 50-150 40-120 30-120

fluoranthene 50-150 40-120 30-120

pyrene 50-150 40-120 30-120

benzo[b]fluorene 50-150 40-120

benz[a]anthracene 50-150 40-120 30-120

chrysene 50-150 40-120 30-120

triphenylene NA 40-120

benzo[b ]fluoranthene 50-150 40-120 30-120

benzo[j ]fluoranthene NA 40-120

benzo[k ]fluoranthene 50-150 40-120 30-120

benzo[a]fluoranthene NA 40-120

benzo[e]pyrene 50-150 40-120 30-120

benzo[a]pyrene 50-150 40-120 30-120

perylene 50-150 40-120 30-120

indeno[1,2,3-cd]pyrene 50-150 40-120 30-120

benzo[ghi]perylene 50-150 40-120 30-120

dibenz[a,h]anthracene 50-150 40-120 30-120

cis/trans-decalin NA 40-120

dibenzofuran NA 40-120

retene NA 40-120

benzothiophene NA 40-120

dibenzothiophene 50-150 40-120 30-120

naphthobenzothiophene NA 40-120
67

Alkylated PAHs - Associated % recovery / acceptance ranges

Lab 1 Lab 2 Lab 3 Lab 4 Lab 5 Lab 6 Lab 7 Lab 8 Lab 9 Lab 10

1-methylnaphthalene 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

2-methylnaphthalene 50-200 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

2,6-dimethylnaphthalene 40%-120%

minus 50 to 

plus 100 50-120 60-140 60-120

1,6,7-trimethylnaphthalene

minus 50 to 

plus 100 50-120 60-140 60-120

1-methylphenanthrene 40%-120%

minus 50 to 

plus 100 50-120 60-140 NA

C1-decalins 40%-120%

minus 50 to 

plus 100 NA NA

C2-decalins 40%-120%

minus 50 to 

plus 100 NA NA

C3-decalins 40%-120%

minus 50 to 

plus 100 NA NA

C4-decalins 40%-120%

minus 50 to 

plus 100 NA NA

C1-naphthalenes 40%-120%

minus 50 to 

plus 100 NA NA

C2-naphthalenes 40%-120%

minus 50 to 

plus 100 50-120 NA NA

C3-naphthalenes 40%-120%

minus 50 to 

plus 100 50-120 NA NA

C4-naphthalenes 40%-120%

minus 50 to 

plus 100 50-120 NA NA

C1-benzothiophenes 40%-120%

minus 50 to 

plus 100 NA NA

C2-benzothiophenes 40%-120%

minus 50 to 

plus 100 NA NA

C3-benzothiophenes 40%-120%

minus 50 to 

plus 100 NA NA

C4-benzothiophenes 40%-120%

minus 50 to 

plus 100 NA NA

C1-fluorenes 40%-120%

minus 50 to 

plus 100 50-120 NA NA

C2-fluorenes 40%-120%

minus 50 to 

plus 100 50-120 NA NA

C3-fluorenes 40%-120%

minus 50 to 

plus 100 50-120 NA NA

C1-phenanthrenes/anthracenes 40%-120%

minus 50 to 

plus 100 50-120 NA NA

C2-phenanthrenes/anthracenes 40%-120%

minus 50 to 

plus 100 50-120 NA NA

C3-phenanthrenes/anthracenes 40%-120%

minus 50 to 

plus 100 50-120 NA NA

C4-phenanthrenes/anthracenes 40%-120%

minus 50 to 

plus 100 50-120 NA NA

C1-dibenzothiophenes 40%-120%

minus 50 to 

plus 100 50-120 NA NA

C2-dibenzothiophenes 40%-120%

minus 50 to 

plus 100 50-120 NA NA

C3-dibenzothiophenes 40%-120%

minus 50 to 

plus 100 50-120 NA NA

C4-dibenzothiophenes 40%-120%

minus 50 to 

plus 100 NA NA

C1-fluoranthenes/pyrenes 40%-120%

minus 50 to 

plus 100 50-120 NA NA

C2-fluoranthenes/pyrenes 40%-120%

minus 50 to 

plus 100 50-120 NA NA

C3-fluoranthenes/pyrenes 40%-120%

minus 50 to 

plus 100 50-120 NA NA

C4-fluoranthenes/pyrenes 40%-120%

minus 50 to 

plus 100 NA NA

C1-naphthobenzothiophenes 40%-120%

minus 50 to 

plus 100 NA NA

C2-naphthobenzothiophenes 40%-120%

minus 50 to 

plus 100 NA NA

C3-naphthobenzothiophenes 40%-120%

minus 50 to 

plus 100 NA NA

C4-naphthobenzothiophenes 40%-120%

minus 50 to 

plus 100 NA NA

C1-chrysenes 40%-120%

minus 50 to 

plus 100 50-120 NA NA

C2-chrysenes 40%-120%

minus 50 to 

plus 100 50-120 NA NA

C3-chrysenes 40%-120%

minus 50 to 

plus 100 50-120 NA NA

C4-chrysenes 40%-120%

minus 50 to 

plus 100 50-120 NA NA
68

Alkylated PAHs - Associated % recovery / acceptance ranges

Lab 11 Lab 12 Lab 13 Lab 14 Lab 15 Lab 16 Lab 17 Lab 18 Lab 19 Lab 20

1-methylnaphthalene 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

2-methylnaphthalene 70 - 130% 70-130 60 - 140

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

2,6-dimethylnaphthalene 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

1,6,7-trimethylnaphthalene 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

1-methylphenanthrene 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C1-decalins 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100%

C2-decalins 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100%

C3-decalins 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100%

C4-decalins 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100%

C1-naphthalenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C2-naphthalenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C3-naphthalenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C4-naphthalenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C1-benzothiophenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100%

C2-benzothiophenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100%

C3-benzothiophenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100%

C4-benzothiophenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100%

C1-fluorenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C2-fluorenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C3-fluorenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C1-phenanthrenes/anthracenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C2-phenanthrenes/anthracenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C3-phenanthrenes/anthracenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C4-phenanthrenes/anthracenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C1-dibenzothiophenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C2-dibenzothiophenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C3-dibenzothiophenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C4-dibenzothiophenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C1-fluoranthenes/pyrenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C2-fluoranthenes/pyrenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C3-fluoranthenes/pyrenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C4-fluoranthenes/pyrenes 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C1-naphthobenzothiophenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100%

C2-naphthobenzothiophenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100%

C3-naphthobenzothiophenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100%

C4-naphthobenzothiophenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100%

C1-chrysenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C2-chrysenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C3-chrysenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140

C4-chrysenes 70 - 130% 70-130

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 60-140
69

Alkylated PAHs - Associated % recovery / acceptance ranges

Lab 21 Lab 22 Lab 23 Lab 24 Lab 25 Lab 26

1-methylnaphthalene 50-150 40-120 30-120

2-methylnaphthalene 50-150 40-120 30-120

2,6-dimethylnaphthalene 50-150 40-120 30-120

1,6,7-trimethylnaphthalene 50-150 40-120

1-methylphenanthrene 50-150 40-120 30-120

C1-decalins NA 40-120

C2-decalins NA 40-120

C3-decalins NA 40-120

C4-decalins NA 40-120

C1-naphthalenes 50-150 40-120 30-120

C2-naphthalenes 50-150 40-120 30-120

C3-naphthalenes 50-150 40-120 30-120

C4-naphthalenes 50-150 40-120 30-120

C1-benzothiophenes NA 40-120

C2-benzothiophenes NA 40-120

C3-benzothiophenes NA 40-120

C4-benzothiophenes NA 40-120

C1-fluorenes 50-150 40-120 30-120

C2-fluorenes 50-150 40-120 30-120

C3-fluorenes 50-150 40-120 30-120

C1-phenanthrenes/anthracenes 50-150 40-120 30-120

C2-phenanthrenes/anthracenes 50-150 40-120 30-120

C3-phenanthrenes/anthracenes 50-150 40-120 30-120

C4-phenanthrenes/anthracenes 50-150 40-120 30-120

C1-dibenzothiophenes 50-150 40-120 30-120

C2-dibenzothiophenes 50-150 40-120 30-120

C3-dibenzothiophenes 50-150 40-120 30-120

C4-dibenzothiophenes NA 40-120 30-120

C1-fluoranthenes/pyrenes 50-150 40-120 30-120

C2-fluoranthenes/pyrenes NA 40-120 30-120

C3-fluoranthenes/pyrenes NA 40-120 30-120

C4-fluoranthenes/pyrenes NA 40-120 30-120

C1-naphthobenzothiophenes NA 40-120

C2-naphthobenzothiophenes NA 40-120

C3-naphthobenzothiophenes NA 40-120

C4-naphthobenzothiophenes NA 40-120

C1-chrysenes 50-150 40-120 30-120

C2-chrysenes 50-150 40-120 30-120

C3-chrysenes 50-150 40-120 30-120

C4-chrysenes 50-150 40-120 30-120
70

Biomarkers - Associated % recovery / acceptance ranges

Lab 1 Lab 2 Lab 3 Lab 4 Lab 5 Lab 6 Lab 7 Lab 8 Lab 9 Lab 10 Lab 11 Lab 12 Lab 13

Carbazole 40%-120%

minus 50 to plus 

100 NA NA 70-130

18a(H)-22,29,30-Trisnorneohopane

minus 50 to plus 

100 NA NA 70 - 130% 70-130

17a(H)-22,29,30-Trisnorhopane

minus 50 to plus 

100 NA NA 70 - 130% 70-130

17α(H),21β(H)-30-Norhopane

minus 50 to plus 

100 NA NA 70 - 130% 70-130

18a(H)-30-Norneohopane

minus 50 to plus 

100 NA NA 70 - 130% 70-130

17a(H)-Diahopane

minus 50 to plus 

100 NA NA 70 - 130% 70-130

17α(H),21β(H)-Hopane 40%-120%

minus 50 to plus 

100 60-140 NA 70 - 130% 70-130

17α(H),21β(H)-22R-Homohopane

minus 50 to plus 

100 NA NA 70 - 130% 70-130

17α(H),21β(H)-22S-Homohopane

minus 50 to plus 

100 NA NA 70 - 130% 70-130

13b(H)17a(H)-Diacholestane 20S

minus 50 to plus 

100 NA NA 70 - 130% 70-130

5a(H),14a(H),17a(H)-Cholestane 20S

minus 50 to plus 

100 NA NA 70 - 130% 70-130

5a(H),14a(H),17a(H)-Cholestane 20R

minus 50 to plus 

100 NA NA 70 - 130% 70-130

5a(H),14a(H),17a(H)-24-Ethylcholestane 20S

minus 50 to plus 

100 NA NA 70 - 130% 70-130

5a(H),14a(H),17a(H)-24-Ethylcholestane 20R

minus 50 to plus 

100 NA NA 70 - 130% 70-130

5a(H),14b(H),17b(H)-Cholestane 20R

minus 50 to plus 

100 NA NA 70 - 130% 70-130

5a(H),14b(H),17b(H)-Cholestane 20S

minus 50 to plus 

100 NA NA 70 - 130% 70-130

5a(H),14b(H),17b(H)-24-Ethylcholestane 20R

minus 50 to plus 

100 NA NA 70 - 130% 70-130

5a(H),14b(H),17b(H)-24-Ethylcholestane 20S

minus 50 to plus 

100 NA NA 70 - 130% 70-130
71

Biomarkers - Associated % recovery / acceptance ranges

Lab 14 Lab 15 Lab 16 Lab 17 Lab 18 Lab 19 Lab 20 Lab 21 Lab 22 Lab 23 Lab 24 Lab 25 Lab 26

Carbazole

50-200% of IS 

value in CCV 40-120

 -50% to + 

100% 50-150 50-130

18a(H)-22,29,30-Trisnorneohopane

50-200% of IS 

value in CCV 60-140 NA 50-130 80-120

17a(H)-22,29,30-Trisnorhopane

50-200% of IS 

value in CCV 60-140 NA 50-130 80-120

17α(H),21β(H)-30-Norhopane

50-200% of IS 

value in CCV

 -50% to + 

100% 60-140 NA 50-130 80-120

18a(H)-30-Norneohopane

50-200% of IS 

value in CCV 60-140 NA 50-130 80-120

17a(H)-Diahopane

50-200% of IS 

value in CCV 60-140 NA 50-130

17α(H),21β(H)-Hopane

50-200% of IS 

value in CCV 40-120 60-140 NA 50-130 80-120

17α(H),21β(H)-22R-Homohopane

50-200% of IS 

value in CCV 60-140 NA 50-130 80-120

17α(H),21β(H)-22S-Homohopane

50-200% of IS 

value in CCV 60-140 NA 50-130 80-120

13b(H)17a(H)-Diacholestane 20S

50-200% of IS 

value in CCV 60-140 NA 50-130

5a(H),14a(H),17a(H)-Cholestane 20S

50-200% of IS 

value in CCV 60-140 NA 50-130 80-120

5a(H),14a(H),17a(H)-Cholestane 20R

50-200% of IS 

value in CCV 60-140 NA 50-130 80-120

5a(H),14a(H),17a(H)-24-Ethylcholestane 20S

50-200% of IS 

value in CCV 60-140 NA 50-130 80-120

5a(H),14a(H),17a(H)-24-Ethylcholestane 20R

50-200% of IS 

value in CCV 60-140 NA 50-130 80-120

5a(H),14b(H),17b(H)-Cholestane 20R

50-200% of IS 

value in CCV 60-140 NA 50-130 80-120

5a(H),14b(H),17b(H)-Cholestane 20S

50-200% of IS 

value in CCV 60-140 NA 50-130 80-120

5a(H),14b(H),17b(H)-24-Ethylcholestane 20R

50-200% of IS 

value in CCV 60-140 NA 50-130 80-120

5a(H),14b(H),17b(H)-24-Ethylcholestane 20S

50-200% of IS 

value in CCV 60-140 NA 50-130 80-120
72

PAHs -If "representative compound" used for quantitation, list the compound

Lab 7 Lab 9 Lab 10 Lab 20 Lab 24

naphthalene NA naphthalene

biphenyl Secondary ion NA biphenyl

acenaphthene Secondary ion NA acenaphthene

acenaphthylene Secondary ion NA acenaphthylene

fluorene Secondary ion NA fluorene

phenanthrene NA phenanthrene

anthracene NA anthracene

fluoranthene NA fluoranthene

pyrene NA pyrene

benzo[b]fluorene NA

benz[a]anthracene NA benz[a]anthracene

chrysene NA chrysene

triphenylene chrysene

benzo[b ]fluoranthene NA benzo[b]fluoranthene

benzo[j ]fluoranthene benzo[k]fluoranthene

benzo[k ]fluoranthene NA benzo[k]fluoranthene benzo[b ]fluoranthene

benzo[a]fluoranthene benzo[k]fluoranthene

benzo[e]pyrene NA benzo[e]pyrene

benzo[a]pyrene NA benzo[a]pyrene

perylene NA perylene

indeno[1,2,3-cd]pyrene NA indeno[1,2,3-cd]pyrene

benzo[ghi]perylene NA benzo[ghi]perylene

dibenz[a,h]anthracene NA dibenz[a,h]anthracene

cis/trans-decalin NA

dibenzofuran NA dibenzofuran

retene 1-methylphenanthrene NA

benzothiophene NA

dibenzothiophene NA dibenzothiophene

naphthobenzothiophene dibenzothiophene NA
73

Alkylated PAHs -If "representative compound" used for quantitation, list the compound

Lab 1 Lab 3 Lab 4 Lab 5 Lab 7 Lab 8 Lab 9

1-methylnaphthalene NA NA

2-methylnaphthalene naphthalene NA NA

2,6-dimethylnaphthalene naphthalene NA NA

1,6,7-trimethylnaphthalene NA NA

1-methylphenanthrene phenanthrene NA NA

C1-decalins cis/trans-decalin NA NA

C2-decalins cis/trans-decalin NA NA

C3-decalins cis/trans-decalin NA NA

C4-decalins cis/trans-decalin NA NA

C1-naphthalenes 1-me and 2-menaphthalene Naphthalene naphthalene Napthalene Naphthalene

C2-naphthalenes 2,6-dimehtylnaphthalene Naphthalene naphthalene Napthalene naphthalene Naphthalene 2,6-dimethylnaphthalene

C3-naphthalenes 2,3,5-trimethylnaphthalene Naphthalene naphthalene Napthalene naphthalene Naphthalene 1,6,7-trimethylnaphthalene

C4-naphthalenes 2,3,5-trimethylnaphthalene Naphthalene naphthalene Napthalene naphthalene Naphthalene 1,6,7-trimethylnaphthalene

C1-benzothiophenes benzothiophene NA NA

C2-benzothiophenes benzothiophene NA NA

C3-benzothiophenes benzothiophene NA NA

C4-benzothiophenes benzothiophene NA NA

C1-fluorenes fluorene Fluorene fluorene Flourene fluorene/secondary ion Fluorene fluorene

C2-fluorenes Fluorene fluorene Flourene fluorene/secondary ion Fluorene fluorene

C3-fluorenes Fluorene fluorene Flourene fluorene/secondary ion Fluorene fluorene

C1-phenanthrenes/anthracenes

1-me, 2-me, 3-me, and 9-

mephenanthrene plus 2-

meanthracene Phenanthrene phenanthrene Phenanthrene anthracene Phenanthrene 1-methylphenanthrene

C2-phenanthrenes/anthracenes 1,7-dimethylphenanthrene Phenanthrene phenanthrene Phenanthrene anthracene Phenanthrene 1-methylphenanthrene

C3-phenanthrenes/anthracenes 1,7-dimethylphenanthrene Phenanthrene phenanthrene Phenanthrene anthracene Phenanthrene 1-methylphenanthrene

C4-phenanthrenes/anthracenes 1,7-dimethylphenanthrene Phenanthrene phenanthrene Phenanthrene anthracene Phenanthrene 1-methylphenanthrene

C1-dibenzothiophenes dibenzothiophene Dibenzothiophene dibenzothiophene Dibenzothiophene dibenzothiophene Dibenzothiophene dibenzothiophene

C2-dibenzothiophenes dibenzothiophene Dibenzothiophene dibenzothiophene Dibenzothiophene dibenzothiophene Dibenzothiophene dibenzothiophene

C3-dibenzothiophenes dibenzothiophene Dibenzothiophene dibenzothiophene Dibenzothiophene dibenzothiophene Dibenzothiophene dibenzothiophene

C4-dibenzothiophenes dibenzothiophene Dibenzothiophene dibenzothiophene Dibenzothiophene Dibenzothiophene dibenzothiophene

C1-fluoranthenes/pyrenes

1-me and 3-mefluoranthene plus 1-

me and 4-mepyrene Pyrene pyrene Pyrene pyrene Pyrene fluoranthene

C2-fluoranthenes/pyrenes

1-me and 3-mefluoranthene plus 1-

me and 4-mepyrene Pyrene pyrene Pyrene pyrene Pyrene fluoranthene

C3-fluoranthenes/pyrenes

1-me and 3-mefluoranthene plus 1-

me and 4-mepyrene Pyrene pyrene Pyrene pyrene Pyrene fluoranthene

C4-fluoranthenes/pyrenes

1-me and 3-mefluoranthene plus 1-

me and 4-mepyrene pyrene NA NA fluoranthene

C1-naphthobenzothiophenes naphthobenzothiophene NA NA dibenzothiophene

C2-naphthobenzothiophenes naphthobenzothiophene NA NA dibenzothiophene

C3-naphthobenzothiophenes naphthobenzothiophene NA NA dibenzothiophene

C4-naphthobenzothiophenes naphthobenzothiophene NA NA dibenzothiophene

C1-chrysenes 3-me and 6-mechyrsne Chrysene chrysene/triphenylene Chyrsene chrysene Chrysene chrysene

C2-chrysenes 3-me and 6-mechyrsne Chrysene chrysene/triphenylene Chyrsene chrysene Chrysene chrysene

C3-chrysenes 3-me and 6-mechyrsne Chrysene chrysene/triphenylene Chyrsene chrysene Chrysene chrysene

C4-chrysenes 3-me and 6-mechyrsne Chrysene chrysene/triphenylene NA chrysene Chrysene chrysene
74

Alkylated PAHs -If "representative compound" used for quantitation, list the compound

Lab 10 Lab 12 Lab 13 Lab 14 Lab 17 Lab 18 Lab 19

1-methylnaphthalene NA

2-methylnaphthalene NA

2,6-dimethylnaphthalene NA

1,6,7-trimethylnaphthalene NA

1-methylphenanthrene phenanthrene Phenanthrene

C1-decalins cis/trans-decalin trans-decalin Decalin trans-Decalin

C2-decalins cis/trans-decalin trans-decalin Decalin trans-Decalin

C3-decalins cis/trans-decalin trans-decalin Decalin trans-Decalin

C4-decalins cis/trans-decalin trans-decalin Decalin trans-Decalin

C1-naphthalenes naphthalene naphthalene Naphthalene 2-methylnaphthalene Naphthalene 2-Methylnaphthalene

C2-naphthalenes naphthalene naphthalene

AVG [2-Ethylnaphtalene/2,6 

dimethylnaphthalene/1,8-

dimethylnaphthalene/1,3-

dimethylnaphthalene] Naphthalene 1-ethylnaphthalene Naphthalene Naphthalene

C3-naphthalenes naphthalene naphthalene 2,3,5-trimethylnaphthalene Naphthalene 2-isopropylnaphthalene Naphthalene Naphthalene

C4-naphthalenes naphthalene naphthalene 1,4,6,7-tetramethylnaphthalene Naphthalene 1,4,6,7-tetramethylnaphthalene Naphthalene Naphthalene

C1-benzothiophenes benzothiophene benzothiophene 4-methylbenzothiophene 4-methylbenzothiophene Benzothiophene Benzothiophene

C2-benzothiophenes benzothiophene benzothiophene 2,3-dimethylbenzothiophene 2,3-dimethylbenzothiophene Benzothiophene Benzothiophene

C3-benzothiophenes benzothiophene benzothiophene

2,3,4/2,3,6-

trimethylbenzothiophenes 2,3,4-trimethylbenzothiophene Benzothiophene Benzothiophene

C4-benzothiophenes benzothiophene benzothiophene __________ Benzothiophene Benzothiophene

C1-fluorenes fluorene fluorene 1-methylfluorene Fluorene 1-methylfluorene Fluorene Fluorene

C2-fluorenes fluorene fluorene Fluorene __________ Fluorene Fluorene

C3-fluorenes fluorene fluorene 9N-propylfluorene Fluorene 9-n-propylfluorene Fluorene Fluorene

C1-phenanthrenes/anthracenes phenanthrene phenanthrene

AVG [1-methylphenanthrene/9-

methylphenanthrene/2methylanthr

acene Phenanthrene 2-methylphenanthrene Phenanthrene Phenanthrene

C2-phenanthrenes/anthracenes phenanthrene phenanthrene 3-ethylphenanthrene Phenanthrene 3-ethylphenanthrene Phenanthrene Phenanthrene

C3-phenanthrenes/anthracenes phenanthrene phenanthrene 1,2,5/1,2,7-trimethylphenanthrene Phenanthrene 1,2,5-trimethylphenanthrene Phenanthrene Phenanthrene

C4-phenanthrenes/anthracenes phenanthrene phenanthrene tertbutylanthracene Phenanthrene __________ Phenanthrene Phenanthrene

C1-dibenzothiophenes dibenzothiophene dibenzothiophene 3-methyldibenzothiophene Dibenzothiophene 3-methyldibenzothiophenes Dibenzothiophene Dibenzothiophene

C2-dibenzothiophenes dibenzothiophene dibenzothiophene 4-ethyldibenzothiophene Dibenzothiophene __________ Dibenzothiophene Dibenzothiophene

C3-dibenzothiophenes dibenzothiophene dibenzothiophene 4-propyldibenzothiophene Dibenzothiophene __________ Dibenzothiophene Dibenzothiophene

C4-dibenzothiophenes dibenzothiophene dibenzothiophene 4,6-diethyldibenzothiophene Dibenzothiophene __________ Dibenzothiophene Dibenzothiophene

C1-fluoranthenes/pyrenes fluoranthene pyrene

[AVG 2-

methylfluoranthene/1methylpyren

e] Pyrene 1-methylpyrene Fluoranthene Fluoranthene

C2-fluoranthenes/pyrenes fluoranthene pyrene Pyrene __________ Fluoranthene Fluoranthene

C3-fluoranthenes/pyrenes fluoranthene pyrene 1N-propylpyrene Pyrene 1,n-propylpyrene Fluoranthene Fluoranthene

C4-fluoranthenes/pyrenes fluoranthene 1N-butylpyrene 1,n-butylpyrene Fluoranthene Fluoranthene

C1-naphthobenzothiophenes naphthobenzothiophene naphthobenzothiophene __________ Naphthobenzothiophene Naphthobenzothiophene

C2-naphthobenzothiophenes naphthobenzothiophene naphthobenzothiophene __________ Naphthobenzothiophene Naphthobenzothiophene

C3-naphthobenzothiophenes naphthobenzothiophene naphthobenzothiophene __________ Naphthobenzothiophene Naphthobenzothiophene

C4-naphthobenzothiophenes naphthobenzothiophene naphthobenzothiophene __________ Naphthobenzothiophene Naphthobenzothiophene

C1-chrysenes chrysene chrysene 6-methylchrysene Chrysene 5-methylchrysene Chrysene Chrysene/Triphenylene

C2-chrysenes chrysene chrysene 6-ethylchrysene Chrysene 6-ethylethylchrysene Chrysene Chrysene/Triphenylene

C3-chrysenes chrysene chrysene Chrysene Chrysene Chrysene/Triphenylene

C4-chrysenes chrysene chrysene 6N-butylchrysene Chrysene Chrysene Chrysene/Triphenylene
75

Alkylated PAHs -If "representative compound" used for quantitation, list the compound

Lab 20 Lab 21 Lab 22 Lab 23 Lab 24 Lab 26

1-methylnaphthalene 1-methylnaphthalene

2-methylnaphthalene 2-methylnaphthalene

2,6-dimethylnaphthalene 2,6-dimethylnaphthalene

1,6,7-trimethylnaphthalene 1,6,7-trimethylnaphthalene naphthalene

1-methylphenanthrene 1-methylphenanthrene

C1-decalins trans-decalin cis/trans-decalin

C2-decalins trans-decalin cis/trans-decalin

C3-decalins trans-decalin cis/trans-decalin

C4-decalins trans-decalin cis/trans-decalin

C1-naphthalenes Naphthalene

1- and 2-Methylnaphthalene are 

added from the individual 

compounds naphthalene 1+2-methylnapthalene Naphthalene

C2-naphthalenes Naphthalene naphthalene naphthalene 2,6-dimethylnaphthalene 2,6-dimethylnaphthalene Naphthalene

C3-naphthalenes Naphthalene naphthalene naphthalene 2,3,5-trimethylnaphthalene 1,6,7-trimethylnaphthalene Naphthalene

C4-naphthalenes Naphthalene naphthalene naphthalene 2,3,5-trimethylnaphthalene 1,6,7-trimethylnaphthalene Naphthalene

C1-benzothiophenes benzothiophene benzothiophene benzothiophene

C2-benzothiophenes benzothiophene benzothiophene benzothiophene

C3-benzothiophenes benzothiophene benzothiophene benzothiophene

C4-benzothiophenes benzothiophene benzothiophene benzothiophene

C1-fluorenes fluorene fluorene fluorene fluorene Fluorene Fluorene

C2-fluorenes fluorene fluorene fluorene fluorene Fluorene Fluorene

C3-fluorenes fluorene fluorene fluorene fluorene Fluorene Fluorene

C1-phenanthrenes/anthracenes phenanthrene phenanthrene phenanthrene 1-methylphenanthrene 1-methylphenanthrene Phenanthrene

C2-phenanthrenes/anthracenes phenanthrene phenanthrene phenanthrene 1-methylphenanthrene 1-methylphenanthrene Phenanthrene

C3-phenanthrenes/anthracenes phenanthrene phenanthrene phenanthrene 1-methylphenanthrene 1-methylphenanthrene Phenanthrene

C4-phenanthrenes/anthracenes phenanthrene phenanthrene phenanthrene 1-methylphenanthrene 1-methylphenanthrene Phenanthrene

C1-dibenzothiophenes dibenzothiophene dibenzotiophene dibenzothiophene dibenzothiophene dibenzothiophene Dibenzothiophene

C2-dibenzothiophenes dibenzothiophene dibenzotiophene dibenzothiophene dibenzothiophene dibenzothiophene Dibenzothiophene

C3-dibenzothiophenes dibenzothiophene dibenzotiophene dibenzothiophene dibenzothiophene dibenzothiophene Dibenzothiophene

C4-dibenzothiophenes dibenzothiophene dibenzothiophene dibenzothiophene dibenzothiophene Dibenzothiophene

C1-fluoranthenes/pyrenes pyrene fluoranthene pyrene pyrene fluoranthene Fluoranthene

C2-fluoranthenes/pyrenes pyrene pyrene pyrene fluoranthene Fluoranthene

C3-fluoranthenes/pyrenes pyrene pyrene pyrene fluoranthene Fluoranthene

C4-fluoranthenes/pyrenes pyrene pyrene pyrene fluoranthene Fluoranthene

C1-naphthobenzothiophenes naphthobenzothiophene naphthobenzothiophene

C2-naphthobenzothiophenes naphthobenzothiophene naphthobenzothiophene

C3-naphthobenzothiophenes naphthobenzothiophene naphthobenzothiophene

C4-naphthobenzothiophenes naphthobenzothiophene naphthobenzothiophene

C1-chrysenes chrysene chrysene chrysene chrysene chrysene+triphenylene Chysene

C2-chrysenes chrysene chrysene chrysene chrysene chrysene+triphenylene Chysene

C3-chrysenes chrysene chrysene chrysene chrysene chrysene+triphenylene Chysene

C4-chrysenes chrysene chrysene chrysene chrysene chrysene+triphenylene Chysene
76

Biomarkers -If "representative compound" used for quantitation, list the compound

Lab 3 Lab 5 Lab 8 Lab 12 Lab 14 Lab 20 Lab 22 Lab 23

Carbazole NA

18a(H)-22,29,30-Trisnorneohopane 17b(H)21b(H) Hopane 17α(H),21β(H)-Hopane 17β(H),21β(H)-Hopane 17β(H),21β(H)-Hopane 17b(H)21b(H) Hopane 17α(H),21β(H)-Hopane 17α(H),21β(H)-Hopane

17a(H)-22,29,30-Trisnorhopane 17b(H)21b(H) Hopane 17α(H),21β(H)-Hopane 17β(H),21β(H)-Hopane 17β(H),21β(H)-Hopane 17b(H)21b(H)Hopane 17b(H)21b(H) Hopane 17α(H),21β(H)-Hopane

17α(H),21β(H)-30-Norhopane 17b(H)21b(H) Hopane 17α(H),21β(H)-Hopane 17β(H),21β(H)-Hopane 17β(H),21β(H)-Hopane 17b(H)21b(H)Hopane 17b(H)21b(H) Hopane 17α(H),21β(H)-Hopane 17α(H),21β(H)-Hopane

18a(H)-30-Norneohopane 17b(H)21b(H) Hopane 17α(H),21β(H)-Hopane 17β(H),21β(H)-Hopane 17β(H),21β(H)-Hopane 17b(H)21b(H)Hopane 17b(H)21b(H) Hopane 17α(H),21β(H)-Hopane 17α(H),21β(H)-Hopane

17a(H)-Diahopane 17b(H)21b(H) Hopane 17α(H),21β(H)-Hopane 17β(H),21β(H)-Hopane 17β(H),21β(H)-Hopane 17b(H)21b(H)Hopane 17b(H)21b(H) Hopane 17α(H),21β(H)-Hopane

17α(H),21β(H)-Hopane 17b(H)21b(H) Hopane 17α(H),21β(H)-Hopane 17β(H),21β(H)-Hopane 17β(H),21β(H)-Hopane 17b(H)21b(H)Hopane 17b(H)21b(H) Hopane

17α(H),21β(H)-22R-Homohopane 17b(H)21b(H) Hopane 17α(H),21β(H)-Hopane 17β(H),21β(H)-Hopane 17β(H),21β(H)-Hopane 17b(H)21b(H)Hopane 17b(H)21b(H) Hopane 17α(H),21β(H)-Hopane 17α(H),21β(H)-Hopane

17α(H),21β(H)-22S-Homohopane 17b(H)21b(H) Hopane 17α(H),21β(H)-Hopane 17β(H),21β(H)-Hopane 17β(H),21β(H)-Hopane 17b(H)21b(H)Hopane 17b(H)21b(H) Hopane 17α(H),21β(H)-Hopane 17α(H),21β(H)-Hopane

13b(H)17a(H)-Diacholestane 20S 5a-Cholestane 5(a) Cholestane 5α - Cholestane ααα 20R-Cholestane 5a-Cholestane 5a-Cholestane 5b(H)Cholane

5a(H),14a(H),17a(H)-Cholestane 20S 5a-Cholestane 5α - Cholestane ααα 20R-Cholestane 5a-Cholestane 5a-Cholestane 5b(H)Cholane

5a(H),14a(H),17a(H)-Cholestane 20R 5a-Cholestane 5(a) Cholestane 5α - Cholestane 5a-Cholestane 5a-Cholestane 5b(H)Cholane 5a(H),14a(H),17a(H)-Cholestane 20S

5a(H),14a(H),17a(H)-24-Ethylcholestane 20S 5a-Cholestane 5(a) Cholestane 5α - Cholestane ααα 20R-Cholestane 5a-Cholestane 5a-Cholestane 5b(H)Cholane 5a(H),14a(H),17a(H)-Cholestane 20S

5a(H),14a(H),17a(H)-24-Ethylcholestane 20R 5a-Cholestane 5(a) Cholestane 5α - Cholestane ααα 20R-Cholestane 5a-Cholestane 5a-Cholestane 5b(H)Cholane 5a(H),14a(H),17a(H)-Cholestane 20S

5a(H),14b(H),17b(H)-Cholestane 20R 5a-Cholestane 5(a) Cholestane 5α - Cholestane ααα 20R-Cholestane 5a-Cholestane 5a-Cholestane 5b(H)Cholane 5a(H),14a(H),17a(H)-Cholestane 20S

5a(H),14b(H),17b(H)-Cholestane 20S 5a-Cholestane 5(a) Cholestane 5α - Cholestane ααα 20R-Cholestane 5a-Cholestane 5a-Cholestane 5b(H)Cholane 5a(H),14a(H),17a(H)-Cholestane 20S

5a(H),14b(H),17b(H)-24-Ethylcholestane 20R 5a-Cholestane 5(a) Cholestane 5α - Cholestane ααα 20R-Cholestane 5a-Cholestane 5a-Cholestane 5b(H)Cholane 5a(H),14a(H),17a(H)-Cholestane 20S

5a(H),14b(H),17b(H)-24-Ethylcholestane 20S 5a-Cholestane 5(a) Cholestane 5α - Cholestane ααα 20R-Cholestane 5a-Cholestane 5a-Cholestane 5b(H)Cholane 5a(H),14a(H),17a(H)-Cholestane 20S
77

APPENDIX C

Laboratory Notes Submitted with the Data
78

Lab Notes

1 QA10OIL01 QA10OIL01 QA10OIL01 SRM 1582 SRM 1582 SRM 1582

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

(μg/g) (μg/g) (μg/g) (μg/g) (μg/g) (μg/g)

chrysene and triphenylene 45.1 46.8 45.8 18.4 17.4 18.1

dibenz[a,c]anthracene 1.92 2.05 1.99 <1 <1 <1

2 Results reported below 500 ug/g are less than the quantitation limit.

3 Method quantification lower limit = 20ug/g

5 RL is 30ug/g. Any values less than 30 will be considered as estimated "J" values.

NA are compounds not calibrated for

DL are compounds that were diluted out.

6 Samples were analyzed at dilutions of 1:100 and 1:1000 to meet calibrated response range

7 ND: Not Detected

8 Quantitation values less than 5.00 ug/g for QA10OIL01 are considered estimated ("J" flag) as these values are below the concentration of the Initial Calibration Curve.

Quantitation values less than 10.0 ug/g for SRM 1582 are considered estimated ("J" flag) as these values are below the concentration of the Initial Calibration Curve.

9 QA10OIL01 QA10OIL01 QA10OIL01 SRM 1582 SRM 1582 SRM 1582 If "representative compound" 

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 IS/surrogate used for quantitation

(μg/g) (μg/g) (μg/g) (μg/g) (μg/g) (μg/g) qunatitation list the compound used here.

chrysene + triphenylene 49.9 55.7 56.1 27.5 26.0 25.6 BaP-d12

benzo[j]fluoranthene + benzo[k]fluoranthene 1.22 1.28 1.32 1.02 0.924 1.01 BaP-d12

dibenz[a,h]anthracene + dibenz[a,c]anthracene 2.10 2.31 2.36 0.809 0.838 0.916 BaP-d12

benzo[b]naphtho[2,1-d]thiophene 18.0 20.0 20.6 17.3 15.9 16.1 ACE-d10 dibenzothiophene

benzo[b]naphtho[1,2-d]thiophene 3.32 3.55 3.65 6.40 6.01 6.06 ACE-d10 dibenzothiophene

benzo[b]naphtho[2,3-d]thiophene 2.33 2.82 2.81 4.55 4.15 4.07 ACE-d10 dibenzothiophene

1,6,7-trimethylnapthalene (also known as 2,3,5-trimethylnaphthalene, CAS # 2245-38-7), partially coelutes with another C3-naphthalene, however this coeluting peak was split off during peak integration.

NPH-d8 recovery range 102-105%

ACE-d10 recovery range 102-106%

BaP-d12 recovery range 108-111%

These recoveries were within the acceptable range of 60-130%

10 <symbol refers to values less than our MDL

The reported value of chrysene is the sum of chrysene and triphenylene

The reported value of benzo(j)fluoranthene is the sum of benzo(k)fluoranthene and benzo(j)fluoranthene
79

Lab Notes

11 Benzo[b]fluroanthene and benzo [j] fluoranthene determined as combined benzo[b&j]fluoranthene; results below(μg/g):

QA10OIL01 QA10OIL01 QA10OIL01 SRM 1582

Sample 1 Sample 2 Sample 3 Sample 1

(μg/g) (μg/g) (μg/g) (μg/g)

Benzo[b&j]fluoranthene 3.6 3.5 3.8 1.7

Biomarkers were measured as peak height ratios only and were not quantitated. These are used diagnostically to match hydrocarbon sources. Ratio results are listed below.

DR-C28 DR-C29 DR-C28+C29 DR-27Ts DR-28ab DR-25nor30ab DR-29Ts DR-30O DR-30G DR-29ab DR-30d

Bottle 113 0.2318 0.2423 0.4741 1.2774 0.0801 0.0000 0.2304 0.0000 0.0291 0.4896 0.1409

Bottle 148 0.2084 0.2234 0.4318 1.2953 0.0771 0.0000 0.2218 0.0000 0.0315 0.4663 0.1303

Bottle 216 0.1925 0.2008 0.3934 1.3395 0.0680 0.0000 0.2084 0.0000 0.0287 0.4620 0.1239

DR-C28 C28 Tricyclic Terpanes / 17a(H),21b(H)-Hopane

DR-C29 C29 Tricyclic Terpanes / 17a(H),21b(H)-Hopane

DR-C28+C29 (C28 Tricyclic Terpanes + C29 Tricyclic Terpanes) / 17a(H),21b(H)-Hopane

DR-27Ts 18a(H)-22,29,30-Trisnorhopane / 17a(H)-22,29,30-Trisnorhopane

DR-28ab 17a(H),21b(H)-28,30-Bisnorhopane / 17a(H),21b(H)-Hopane

DR-25nor30ab 17a(H)21b(H)-25-Norhopane / 17a(H)21b(H)-Hopane

DR-29Ts 18a(H)-30-Norneohopane / 17a(H),21b(H)-Hopane

DR-30O 18a(H)-3Oleane / 17a(H),21b(H)-Hopane

DR-30G Gammacerane / 17a(H),21b(H)-Hopane

DR-29ab 17a(H),21b(H)-30-Norhopane / 17a(H),21b(H)-Hopane

DR-30d 15a-methyl-17a(H)-27-Norhopane / 17a(H),21b(H)-Hopane

DR-29aaS 24-ethyl-5a(H),14a(H),17a,20S-cholestane / 24-ethyl-5a(H),14a(H),17a,20R-cholestane

DR-29bb (24-ethyl-5a(H),14b(H),17b,20S-cholestane + 24-ethyl-5a(H),14b(H),17b,20R-cholestane / (24-ethyl-5a(H),14a(H),17a,20S-cholestane + 24-ethyl-5a(H),14a(H),17a,20R-cholestane)

D-27bbSTER 5a(H),14b(H),17b,20(S+R)-cholestane / (24-methyl-5a(H),14b(H),17b,20(S+R)-cholestane + 24-ethyl-5a(H),14b(H),17b,20(S+R)-cholestane)

D-28bbSTER 24-methyl-5a(H),14b(H),17b,20(S+R)-cholestane / (5a(H),14b(H),17b,20(S+R)-cholestane + 24-ethyl-5a(H),14b(H),17b,20(S+R)-cholestane)

D-29bbSTER 24-ethyl-5a(H),14b(H),17b,20(S+R)-cholestane / (5a(H),14b(H),17b,20(S+R)-cholestane + 24-ethyl-5a(H),14b(H),17b,20(S+R)-cholestane)

DR-SES1-2 C14-sesquiterpane(1) / C14-sesquiterpane(2)1

DR-SES3-5 C15-sesquiterpane(3) / C15-8b(H)-drimane(5)1

DR-SES4-6 C15-sesquiterpane(1) / C15-sesquiterpane(2)1

DR-SES5-10 C15-8b(H)-drimane(5) / C16-8b(H)-homodrimane(10)1

1

Identification and numbering from Oil spill identification - Waterborne petroleum and petroeum products - Part 2: Analytical methodology and interpretation of results. CEN/TR 15522-2:2006: E

13 QA10OIL01 QA10OIL01 QA10OIL01 SRM 1582

Sample 1 Sample 2 Sample 3 Sample 1

(μg/g) (μg/g) (μg/g) (μg/g)

Methyl Cholestane 10.6 7.85 8.02 51.1

17β(H),21α(H)-Hopane 12 5.97 10.6 73.7

Diagnostic Ratios of Hopanes (m/e 191)
80

Lab Notes

13 (cont) QA10OIL01 QA10OIL01

Sample 4 Sample 5

Sample Jar number 309 366

(μg/g) (μg/g)

naphthalene 1050 1050

biphenyl 211 202

acenaphthene 85.3 83

acenaphthylene 13.2 11.1

fluorene 162 165

phenanthrene 375 378

anthracene 28.6 24.1

fluoranthene 5.32 8.78

pyrene 14.9 16.6

benzo[b]fluorene 8.41 9.64

benz[a]anthracene 5.88 6.36

chrysene 64.7 63.8

triphenylene 35.8 35.2

benzo[b ]fluoranthene 7.1 7.65

benzo[j]fluoranthene 1.5 1.7

benzo[k ]fluoranthene 0.564 0.594

benzo[a]fluoranthene N/A N/A

benzo[e]pyrene 9.56 8.55

benzo[a]pyrene 2 1.21

perylene 0.315 0.523

indeno[1,2,3-cd]pyrene 216 216

benzo[ghi]perylene 6.49 2.52

dibenz[a,h]anthracene N/A N/A

cis/trans-decalin 1290 1280

dibenzofuran 35.9 36.3

retene 11 12.8

benzothiophene 20.7 19.7

dibenzothiophene 47.7 49.9

naphthobenzothiophene 1.87 2

1-methylnaphthalene 1050 1050

2-methylnaphthalene 1720 1730

2,6-dimethylnaphthalene 204 193

1,6,7-trimethylnaphthalene N/A N/A

1-methylphenanthrene 119 121

C1-decalins N/A N/A

C2-decalins N/A N/A

C3-decalins N/A N/A

C4-decalins N/A N/A

C1-naphthalenes 2770 2780

C2-naphthalenes 3090 3090

C3-naphthalenes 2040 2070

C4-naphthalenes 281 393

C1-benzothiophenes 218 223

C2-benzothiophenes 4160 4990

C3-benzothiophenes 309 385

C4-benzothiophenes N/A N/A

C1-fluorenes 621 655

C2-fluorenes N/A N/A

C3-fluorenes 538 639

C1-phenanthrenes/anthracenes 832 888

C2-phenanthrenes/anthracenes 740 895

C3-phenanthrenes/anthracenes 169 195

C4-phenanthrenes/anthracenes 68.4 70.4

C1-dibenzothiophenes 4.99 8.75

C2-dibenzothiophenes 104 103

C3-dibenzothiophenes 74.5 82.7

C4-dibenzothiophenes 45.1 51.4

C1-fluoranthenes/pyrenes 52.7 50.4

C2-fluoranthenes/pyrenes N/A N/A

C3-fluoranthenes/pyrenes 55.5 62.7

C4-fluoranthenes/pyrenes 5.75 10.4

C1-naphthobenzothiophenes N/A N/A

C2-naphthobenzothiophenes N/A N/A

C3-naphthobenzothiophenes N/A N/A

C4-naphthobenzothiophenes N/A N/A

C1-chrysenes 180 182

C2-chrysenes 15.7 15.9

C3-chrysenes N/A N/A

C4-chrysenes 4.38 4.4

Carbazole 9.66 9.2

18a(H)-22,29,30-Trisnorhopane N/A N/A

17a(H)-22,29,30-Trisnorhopane 28.7 27.1

17α(H),21β(H)-30-Norhopane N/A N/A

18a(H)-30-Norneohopane N/A N/A

17a(H)-Diahopane N/A N/A

17α(H),21β(H)-Hopane 43.2 44.4

17α(H),21β(H)-22R-Homohopane N/A N/A

17α(H),21β(H)-22S-Homohopane N/A N/A

13b(H)17a(H)-Diacholestane 20S N/A N/A

5a(H),14a(H),17a(H)-Cholestane 20S N/A N/A

5a(H),14a(H),17a(H)-Cholestane 20R 46.9 33.5

5a(H),14a(H),17a(H)-24-Ethylcholestane 20S N/A N/A

5a(H),14a(H),17a(H)-24-Ethylcholestane 20R 15.8 15.5

5a(H),14b(H),17b(H)-Cholestane 20R N/A N/A

5a(H),14b(H),17b(H)-Cholestane 20S N/A N/A

5a(H),14b(H),17b(H)-24-Ethylcholestane 20R N/A N/A

5a(H),14b(H),17b(H)-24-Ethylcholestane 20S N/A N/A

Methyl Cholestane 8.21 10.7

17β(H),21α(H)-Hopane 5.24 11.2
81

Lab Notes

18 * Triphenylene cannot be resolved from Chrysene under the chromatographic conditions used

** Benzo(j)fluoranthene cannot be resolved from Benzo(k)fluoranthene under the chromatographic condition used

Other Detection limit was elevated sue to matrix interference.

19 QA10OIL01 QA10OIL01 QA10OIL01 SRM 1582 SRM 1582 SRM 1582

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 IS/surrogate Associated % recovery

(μg/g) (μg/g) (μg/g) (μg/g) (μg/g) (μg/g) qunatitation acceptance ranges

4-Methyldibenzothiophene 128 106 126 76.2 65.6 89.1 Phen-d10 -50% to + 100%

2/3-Methyldibenzothiophene 51 43.3 50.2 41 36.3 50.7 Phen-d10 -50% to + 100%

1-Methyldibenzothiophene 34.2 29.4 34.2 36.8 32.1 42.9 Phen-d10 -50% to + 100%

3-Methylphenanthrene 238 181 209 94.5 83.6 115 Phen-d10 -50% to + 100%

2-Methylphenanthrene 94.8 80.7 94.9 49.6 44 54.1 Phen-d10 -50% to + 100%

2-Methylanthracene <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 Phen-d10 -50% to + 100%

4/9-Methylphenanthrene 136 109 126 55.1 55 64.9 Phen-d10 -50% to + 100%

2-Chloronaphthalene <0.02 <0.02 <0.02 <0.02 <0.02 <0.02 Acenaphthalene-d10 -50% to + 100%

(chrysene + triphenylene) 43.9 37.3 43.9 11.2 9.41 17.9

benzo (A and B)fluoranthene 5.22 4.09 4.85 <0.02 <0.02 <0.02 

21 * We do have performance based ranges for recoveries but we keep the 50 to 150% for consistency with the original method.

We have not conducted an MDL study with oils so the reporting limits are estimated at 5 ug/g, below this point the uncertainity of the confirmation ion ratios become too great. 

We analyzed larger amounts of the samples to improve the detection but is not our normal procedure.

The lowest calibration solution is equivalent to 2ug/g.

Because this is a direct injection method all samples were analyzed in triplicates in one single batch.

GC/MS analyses were conducted in different days with different calibrations.

Both samples were treated in identical manner. Concnetrated samples (2.5x and 5x the regular analyzed amounts) were used to determine PAHs with lower concentrations (HMW-PAHs)

22 1) chrysene is reported as a coelution of both chrysene and triphenylene.

2) benzo[k]fluoranthene is reported as a coelution of both benzo[j]fluoranthene and benzo[k]fluoranthene.
82

Lab Notes

24 Most data reported here are for 1% dilutions of QA10OIL and SRM1582. We only analyzed 1582 once at this level. Where data are reported for three 1582 samples, the values come from analysis of samples diluted 1 part per thousand. 

Figures lacking three significant figures should be assumed to have lost trailing zeroes.

Chrysene and triphenylene quantified together as one chromatographic peak.

Benzo[j]fluoranthene and benzo[k]fluoranthene quantified together as one chromatographic peak.

2,6- and 2,7-methylnaphthalene were quantified together (although we do not assume both are present in samples) as one chromatographic peak.

Cis- and trans-decalin were mostly, if not all, trans-decalin. 

Detection limits were roughly 5 to 10 ug/g.

Other: See calibration nonconformances. If "reprentative compound" 

Other analytes: IS/surrogate used for quantitation

QA10OIL-169 QA10OIL-327 QA10OIL-349 SRM 1582 SRM 1582 SRM 1582 qunatitation list the compound used here.

1,3-Dimethylnapthalene 985 937 1041 313 NA NA d8-Acenapthylene

1,6-Dimethylnapthalene 554 443 455 378 NA NA d10-Acenapthene

1,4-Dimethylnapthalene 233 216 230 168 NA NA d10-Acenapthene

1,5-Dimethylnapthalene 128 116 136 76 NA NA d10-Acenapthene

1,2-Dimethylnapthalene 167 183 180 107 NA NA d8-Acenapthylene

1,8-Dimethylnapthalene DL DL DL DL NA NA d8-Acenapthylene

1,4,6-Trimethylnapthalene 578 699 551 400 NA NA d8-Acenapthylene 1,6,7-trimethylnaphthalene

2,3,6-Trimethylnapthalene 302 378 352 261 NA NA d8-Acenapthylene

1,2,5-Trimethylnapthalene 167 221 256 191 NA NA d8-Acenapthylene 1,6,7-trimethylnaphthalene

1,4,6,7-Tetramethylnaphthalene 248 230 361 218 NA NA d10-Phenanthrene 2,3,6-trimethylnaphthalene

1-MeFluorene 172 185 203 109 NA NA d10-Fluorene

1,2,5,6-Tetramethylnaphthalene 98.2 85.1 129 94.5 NA NA d10-Phenanthrene 2,3,6-trimethylnaphthalene

4-MeDibenzothiophene 87.1 86.7 86.4 59.1 NA NA d8-Dibenzothiophene

2-MeDibenzothiophene 29.2 32.6 31.3 33.6 NA NA d8-Dibenzothiophene

3-MePhenanthrene 246 218 366 114 NA NA d10-Phenanthrene

2-MePhenanthrene 262 260 261 138 NA NA d10-Anthracene

2-MeAnthracene DL DL DL 5.52 NA NA d10-Fluoranthene

1-MeAnthracene 327 330 314 136 NA NA d10-Anthracene

Retene 19.8 21.6 19.8 59 NA NA d10-Pyrene

11H-Benzo[a]fluorene 37.2 37.7 36.6 DL NA NA d10-Pyrene

C1-Benzofluoranthenes/benzopyrenes 49.6 49.2 50.4 32.5 NA NA d12-Benzo(e)pyrene benzo[e]pyrene

C2-Benzofluoranthenes/benzopyrenes 56.3 48.7 58.6 35.6 NA NA d12-Benzo(e)pyrene benzo[e]pyrene

C3-Benzofluoranthenes/benzopyrenes 46 45 44.2 31 NA NA d12-Benzo(e)pyrene benzo[e]pyrene

C4-Benzofluoranthenes/benzopyrenes 32.9 33.5 33.6 43.8 NA NA d12-Benzo(e)pyrene benzo[e]pyrene

cis/trans-decalin 436 370 310 208 NA NA

C1-decalins 1250 878 744 694 NA NA

25 QA10OIL01 QA10OIL01 QA10OIL01 SRM 1582 SRM 1582 SRM 1582

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 IS/surrogate 

(μg/g) (μg/g) (μg/g) (μg/g) (μg/g) (μg/g) qunatitation

abb 20R 24S-methylcholestane 24.1 19.6 21.9 220 249 224 n-triacontane-d62

4-methylpyrene 25.2 25.5 22.8 14.7 15.9 15.3 fluoranthene-d10

1-methylpyrene 14.6 14.1 14.3 10.7 11.1 10.7 fluoranthene-d10

3-methylchrysene 18.7 18.0 18.3 6.73 6.77 6.49 fluoranthene-d10

6-methylchrysene 15.1 14.4 15.1 7.49 7.50 6.80 fluoranthene-d10

3-methylphenanthrene 262 248 247 114 120 117 phenanthrene-d10

2-methylphenanthrene 224 213 213 71 109 102 phenanthrene-d10

2-methylanthracene 23.4 24.9 24.4 10.4 11.0 11.2 phenanthrene-d10

9-methylphenanthrene 265 252 251 107 113 108 phenanthrene-d10

2,6-dimethylphenanthrene 159 162 164 150 149 146 phenanthrene-d10

benzo[b]chrysene 0.614 0.621 0.626 0.302 0.289 0.272 benzo[ghi]perylene-d12

picene 2.17 2.18 2.24 0.925 0.904 0.783 benzo[ghi]perylene-d12

dibenzo[ah]pyrene 1.27 1.19 1.20 0.439 0.430 0.478 benzo[ghi]perylene-d12
83

APPENDIX D

Charts of QA10OIL01 and SRM 1582

Results by Analyte

See Tables 1 through 3 for results reported as <number, detection limit, etc. 

For QA10OIL01 plots:

Solid line: exercise assigned value

Dotted line: z = ± 1, i. e., 25 % from assigned value

Dotted/dashed line: z = ± 2, i. e., 50 % from assigned value

Dashed line: z = ± 3, i. e., 75 % from assigned value

For SRM 1582 plots:

Solid line: material certified concentration or target value (see caption of each plot)

Dotted line: 95 % confidence interval (CI)

Dashed line: 30 % from 95 % confidence interval (CI)
84

0

200

400

600

800

1000

1200

1400

1600

laboratory number

 mass fraction (ug/g)

naphthalene QA10OIL01

Assigned mean = 837 ug/g s = 126 ug/g 95% CI = 48 ug/g Assigned median = 820 ug/g 

Reported Results: 26 Quantitative Results: 26 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

50

100

150

200

250

300

laboratory number

mass fraction (ug/g)

naphthalene SRM 1582

Target Value = no target ug/g 

Reported Results: 25 Quantitative Results: 25 Median of Reported Results: 164 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
85

0

50

100

150

200

250

300

350

400

laboratory number

 mass fraction (ug/g)

biphenyl QA10OIL01

Assigned mean = 194 ug/g s = 34 ug/g 95% CI = 13 ug/g Assigned median = 181 ug/g 

Reported Results: 25 Quantitative Results: 25 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

10.0

20.0

30.0

40.0

50.0

60.0

laboratory number

mass fraction (ug/g)

biphenyl SRM 1582

Target Value = no target ug/g 

Reported Results: 25 Quantitative Results: 24 Median of Reported Results: 27.9 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
86

0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

laboratory number

 mass fraction (ug/g)

acenaphthene QA10OIL01

Assigned mean = 16.4 ug/g s = 7.2 ug/g 95% CI = 3.3 ug/g Assigned median = 14.0 ug/g 

Reported Results: 26 Quantitative Results: 19 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

laboratory number

mass fraction (ug/g)

acenaphthene SRM 1582

Target Value = no target ug/g 

Reported Results: 25 Quantitative Results: 22 Median of Reported Results: 18.8 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
87

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

18.0

20.0

laboratory number

 mass fraction (ug/g)

acenaphthylene QA10OIL01

Assigned mean = 10.1 ug/g s = 3.8 ug/g 95% CI = 2.0 ug/g Assigned median = 8.3 ug/g 

Reported Results: 26 Quantitative Results: 15 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

18.0

20.0

laboratory number

mass fraction (ug/g)

acenaphthylene SRM 1582

Target Value = no target ug/g 

Reported Results: 25 Quantitative Results: 16 Median of Reported Results: 8.47 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
88

0

50

100

150

200

250

300

laboratory number

 mass fraction (ug/g)

fluorene QA10OIL01

Assigned mean = 140 ug/g s = 28 ug/g 95% CI = 11 ug/g Assigned median = 139 ug/g 

Reported Results: 26 Quantitative Results: 26 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

10.0

20.0

30.0

40.0

50.0

60.0

laboratory number

mass fraction (ug/g)

fluorene SRM 1582

Target Value = no target ug/g 

Reported Results: 25 Quantitative Results: 23 Median of Reported Results: 32.0 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
89

0

100

200

300

400

500

600

laboratory number

 mass fraction (ug/g)

phenanthrene QA10OIL01

Assigned mean = 281 ug/g s = 42 ug/g 95% CI = 16 ug/g Assigned median = 277 ug/g 

Reported Results: 26 Quantitative Results: 26 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

20

40

60

80

100

120

140

160

laboratory number

mass fraction (ug/g)

phenanthrene SRM 1582

certified Value = 101 ug/g ; 95% CI 5 ug/g

Reported Results: 25 Quantitative Results: 24 Median of Reported Results: 103 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
90

0.0

5.0

10.0

15.0

20.0

25.0

laboratory number

 mass fraction (ug/g)

anthracene QA10OIL01

Assigned mean = 8.79 ug/g s = 4.79 ug/g 95% CI = 2.42 ug/g Assigned median = 7.52 ug/g 

Reported Results: 26 Quantitative Results: 16 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

5.0

10.0

15.0

20.0

25.0

laboratory number

mass fraction (ug/g)

anthracene SRM 1582

Target Value = no target ug/g 

Reported Results: 25 Quantitative Results: 12 Median of Reported Results: 7.20 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
91

0.0

2.0

4.0

6.0

8.0

10.0

12.0

laboratory number

 mass fraction (ug/g)

fluoranthene QA10OIL01

Assigned mean = 4.72 ug/g s = 1.36 ug/g 95% CI = 0.64 ug/g Assigned median = 4.44 ug/g 

Reported Results: 26 Quantitative Results: 18 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

9.00

laboratory number

mass fraction (ug/g)

fluoranthene SRM 1582

certified Value = 2.5 ug/g ; 95% CI 0.3 ug/g

Reported Results: 25 Quantitative Results: 17 Median of Reported Results: 3.7 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
92

0.0

5.0

10.0

15.0

20.0

25.0

30.0

laboratory number

 mass fraction (ug/g)

pyrene QA10OIL01

Assigned mean = 15.5 ug/g s = 3.5 ug/g 95% CI = 1.4 ug/g Assigned median = 15.3 ug/g 

Reported Results: 26 Quantitative Results: 23 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

18.0

20.0

laboratory number

mass fraction (ug/g)

pyrene SRM 1582

Target Value = no target ug/g 

Reported Results: 25 Quantitative Results: 21 Median of Reported Results: 10.3 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
93

0.0

5.0

10.0

15.0

20.0

25.0

30.0

laboratory number

 mass fraction (ug/g)

benzo[b]fluorene QA10OIL01

Assigned mean = 14.9 ug/g s = 4.7 ug/g 95% CI = 3.3 ug/g Assigned median = 13.0 ug/g 

Reported Results: 11 Quantitative Results: 8 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

laboratory number

mass fraction (ug/g)

benzo[b]fluorene SRM 1582

Target Value = no target ug/g 

Reported Results: 11 Quantitative Results: 6 Median of Reported Results: 9.38 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
94

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

18.0

laboratory number

 mass fraction (ug/g)

benz[a]anthracene QA10OIL01

Assigned mean = 8.40 ug/g s = 3.47 ug/g 95% CI = 1.56 ug/g Assigned median = 7.68 ug/g 

Reported Results: 26 Quantitative Results: 19 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

laboratory number

mass fraction (ug/g)

benz[a]anthracene SRM 1582

certified Value = 3.0 ug/g ; 95% CI 0.3 ug/g

Reported Results: 25 Quantitative Results: 16 Median of Reported Results: 3.50 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
95

0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

laboratory number

 mass fraction (ug/g)

chrysene QA10OIL01

Assigned value = No assigned value 

Reported Results: 18 Quantitative Results: 17 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

5.0

10.0

15.0

20.0

25.0

30.0

laboratory number

mass fraction (ug/g)

chrysene SRM 1582

Target Value = no target ug/g 

Reported Results: 17 Quantitative Results: 14 Median of Reported Results: not calculated

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
96

0.0

10.0

20.0

30.0

40.0

50.0

60.0

laboratory number

 mass fraction (ug/g)

triphenylene QA10OIL01

Assigned value = No assigned value 

Reported Results: 4 Quantitative Results: 3 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

18.0

20.0

laboratory number

mass fraction (ug/g)

triphenylene SRM 1582

Target Value = no target ug/g 

Reported Results: 4 Quantitative Results: 3 Median of Reported Results: not calculated

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
97

0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

80.0

90.0

laboratory number

 mass fraction (ug/g)

chrysene + triphenylene QA10OIL01

Assigned mean = 47.7 ug/g s = 4.7 ug/g 95% CI = 3.3 ug/g Assigned median = 47.2 ug/g 

Reported Results: 8 Quantitative Results: 8 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

5.0

10.0

15.0

20.0

25.0

30.0

laboratory number

mass fraction (ug/g)

chrysene + triphenylene SRM 1582

 Value = no target ug/g 

Reported Results: 8 Quantitative Results: 8 Median of Reported Results: 20.1 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
98

0.0

2.0

4.0

6.0

8.0

10.0

12.0

laboratory number

 mass fraction (ug/g)

benzo[b]fluoranthene QA10OIL01

Assigned mean = 6.13 ug/g s = 1.13 ug/g 95% CI = 0.51 ug/g Assigned median = 5.83 ug/g 

Reported Results: 23 Quantitative Results: 19 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

18.0

laboratory number

mass fraction (ug/g)

benzo[b]fluoranthene SRM 1582

Target Value = no target ug/g 

Reported Results: 21 Quantitative Results: 13 Median of Reported Results: 2.32 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
99

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

laboratory number

 mass fraction (ug/g)

benzo[j]fluoranthene QA10OIL01

Assigned value = No assigned value 

Reported Results: 6 Quantitative Results: 4 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

2.0

4.0

6.0

8.0

10.0

12.0

laboratory number

mass fraction (ug/g)

benzo[j]fluoranthene SRM 1582

Target Value = no target ug/g 

Reported Results: 5 Quantitative Results: 4 Median of Reported Results: 0.858 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
100

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

laboratory number

 mass fraction (ug/g)

benzo[k]fluoranthene QA10OIL01

Assigned value = No assigned value 

Reported Results: 19 Quantitative Results: 4 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

18.0

laboratory number

mass fraction (ug/g)

benzo[k]fluoranthene SRM 1582

Target Value = no target ug/g 

Reported Results: 18 Quantitative Results: 5 Median of Reported Results: 1.57 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
101

0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

laboratory number

 mass fraction (ug/g)

benzo[j]fluoranthene + benzo[k]fluoranthene QA10OIL01

Assigned value = No assigned value 

Reported Results: 4 Quantitative Results: 2 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.20

0.40

0.60

0.80

1.00

1.20

laboratory number

mass fraction (ug/g)

benzo[j]fluoranthene + benzo[k]fluoranthene SRM 1582

 Value = no target ug/g 

Reported Results: 4 Quantitative Results: 2 Median of Reported Results: 0.873 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
102

0.00

5.00

10.00

15.00

20.00

25.00

laboratory number

 mass fraction (ug/g)

benzo[e]pyrene QA10OIL01

Assigned mean = 11.7 ug/g s = 1.8 ug/g 95% CI = 0.8 ug/g Assigned median = 11.3 ug/g 

Reported Results: 25 Quantitative Results: 21 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

laboratory number

mass fraction (ug/g)

benzo[e]pyrene SRM 1582

Reference Value = 3.5 ug/g ; 95% CI 0.5 ug/g

Reported Results: 25 Quantitative Results: 15 Median of Reported Results: 3.86 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
103

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

4.00

laboratory number

 mass fraction (ug/g)

benzo[a]pyrene QA10OIL01

Assigned mean = 1.94 ug/g s = 0.50 ug/g 95% CI = 0.32 ug/g Assigned median = 2.10 ug/g 

Reported Results: 26 Quantitative Results: 10 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.50

1.00

1.50

2.00

2.50

3.00

laboratory number

mass fraction (ug/g)

benzo[a]pyrene SRM 1582

certified Value = 1.1 ug/g ; 95% CI 0.3 ug/g

Reported Results: 25 Quantitative Results: 11 Median of Reported Results: 1.34 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
104

0.00

0.20

0.40

0.60

0.80

1.00

1.20

1.40

1.60

1.80

2.00

laboratory number

 mass fraction (ug/g)

perylene QA10OIL01

Assigned mean = 0.838 ug/g s = 0.450 ug/g 95% CI = 0.279 ug/g Assigned median = 0.694 ug/g 

Reported Results: 25 Quantitative Results: 10 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

laboratory number

mass fraction (ug/g)

perylene SRM 1582

certified Value = 31.0 ug/g ; 95% CI 3.0 ug/g

Reported Results: 25 Quantitative Results: 23 Median of Reported Results: 32.4

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
105

0.000

0.100

0.200

0.300

0.400

0.500

0.600

0.700

laboratory number

 mass fraction (ug/g)

indeno[1,2,3-cd]pyrene QA10OIL01

Assigned value = No assigned value 

Reported Results: 26 Quantitative Results: 3 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

0.80

0.90

1.00

laboratory number

mass fraction (ug/g)

indeno[1,2,3-cd]pyrene SRM 1582

Target Value = no target ug/g 

Reported Results: 25 Quantitative Results: 3 Median of Reported Results: not calculated

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
106

0.00

1.00

2.00

3.00

4.00

5.00

6.00

laboratory number

 mass fraction (ug/g)

benzo[ghi]perylene QA10OIL01

Assigned mean = 2.10 ug/g s = 0.49 ug/g 95% CI = 0.29 ug/g Assigned median = 2.01 ug/g 

Reported Results: 26 Quantitative Results: 12 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

1.00

2.00

3.00

4.00

5.00

6.00

laboratory number

mass fraction (ug/g)

benzo[ghi]perylene SRM 1582

Reference Value = 1.7 ug/g ; 95% CI 0.1 ug/g

Reported Results: 25 Quantitative Results: 12 Median of Reported Results: 1.97 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
107

0.00

0.50

1.00

1.50

2.00

2.50

3.00

3.50

laboratory number

 mass fraction (ug/g)

dibenz[a,h]anthracene QA10OIL01

Assigned value = No assigned value 

Reported Results: 24 Quantitative Results: 12 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.00

0.50

1.00

1.50

2.00

2.50

3.00

laboratory number

mass fraction (ug/g)

dibenz[a,h]anthracene SRM 1582

Target Value = no target ug/g 

Reported Results: 22 Quantitative Results: 8 Median of Reported Results: 1.22 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
108

0

200

400

600

800

1000

1200

1400

1600

laboratory number

 mass fraction (ug/g)

cis/trans-decalin QA10OIL01

Assigned mean = 856 ug/g s = 302 ug/g 95% CI = 198 ug/g Assigned median = 704 ug/g 

Reported Results: 9 Quantitative Results: 9 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

100

200

300

400

500

600

700

laboratory number

mass fraction (ug/g)

cis/trans-decalin SRM 1582

Target Value = no target ug/g 

Reported Results: 9 Quantitative Results: 9 Median of Reported Results: 400 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
109

0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

45.0

50.0

laboratory number

 mass fraction (ug/g)

dibenzofuran QA10OIL01

Assigned mean = 26.7 ug/g s = 5.9 ug/g 95% CI = 3.2 ug/g Assigned median = 25.7 ug/g 

Reported Results: 18 Quantitative Results: 13 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

45.0

laboratory number

mass fraction (ug/g)

dibenzofuran SRM 1582

Target Value = no target ug/g 

Reported Results: 17 Quantitative Results: 14 Median of Reported Results: 8.87 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
110

0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

80.0

laboratory number

 mass fraction (ug/g)

retene QA10OIL01

Assigned mean = 16.7 ug/g s = 6.3 ug/g 95% CI = 4.7 ug/g Assigned median = 19.8 ug/g 

Reported Results: 11 Quantitative Results: 8 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

80.0

laboratory number

mass fraction (ug/g)

retene SRM 1582

Target Value = no target ug/g 

Reported Results: 12 Quantitative Results: 10 Median of Reported Results: 44.5 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
111

0.0

5.0

10.0

15.0

20.0

25.0

laboratory number

 mass fraction (ug/g)

benzothiophene QA10OIL01

Assigned mean = 13.6 ug/g s = 7.3 ug/g 95% CI = 5.1 ug/g Assigned median = 9.68 ug/g 

Reported Results: 11 Quantitative Results: 8 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

laboratory number

mass fraction (ug/g)

benzothiophene SRM 1582

Target Value = no target ug/g 

Reported Results: 9 Quantitative Results: 9 Median of Reported Results: 8.31 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
112

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laboratory number

 mass fraction (ug/g)

dibenzothiophene QA10OIL01

Assigned mean = 49.1 ug/g s = 6.6 ug/g 95% CI = 2.7 ug/g Assigned median = 49.3 ug/g 

Reported Results: 25 Quantitative Results: 24 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

80.0

laboratory number

mass fraction (ug/g)

dibenzothiophene SRM 1582

certified Value = 33 ug/g ; 95% CI 2 ug/g

Reported Results: 25 Quantitative Results: 24 Median of Reported Results: 36.8 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
113

0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

laboratory number

 mass fraction (ug/g)

naphthobenzothiophene QA10OIL01

Assigned mean = 12.3 ug/g s = 7.0 ug/g 95% CI = 4.8 ug/g Assigned median = 14.6 ug/g 

Reported Results: 10 Quantitative Results: 9 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

45.0

laboratory number

mass fraction (ug/g)

naphthobenzothiophene SRM 1582

Target Value = no target ug/g 

Reported Results: 10 Quantitative Results: 9 Median of Reported Results: 13.5 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
114

0

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laboratory number

 mass fraction (ug/g)

1-methylnaphthalene QA10OIL01

Assigned mean = 1102 ug/g s = 137 ug/g 95% CI = 56 ug/g Assigned median = 1100 ug/g 

Reported Results: 24 Quantitative Results: 24 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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300

400

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laboratory number

mass fraction (ug/g)

1-methylnaphthalene SRM 1582

Target Value = no target ug/g 

Reported Results: 24 Quantitative Results: 24 Median of Reported Results: 351 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
115

0

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laboratory number

 mass fraction (ug/g)

2-methylnaphthalene QA10OIL01

Assigned mean = 1623 ug/g s = 259 ug/g 95% CI = 101 ug/g Assigned median = 1621 ug/g 

Reported Results: 25 Quantitative Results: 25 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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300

400

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600

700

laboratory number

mass fraction (ug/g)

2-methylnaphthalene SRM 1582

Target Value = no target ug/g 

Reported Results: 24 Quantitative Results: 24 Median of Reported Results: 528 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
116

0

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1800

laboratory number

 mass fraction (ug/g)

2,6-dimethylnaphthalene QA10OIL01

Assigned mean = 916 ug/g s = 293 ug/g 95% CI = 122 ug/g Assigned median = 997 ug/g 

Reported Results: 23 Quantitative Results: 22 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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300

400

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600

700

laboratory number

mass fraction (ug/g)

2,6-dimethylnaphthalene SRM 1582

Target Value = no target ug/g 

Reported Results: 23 Quantitative Results: 21 Median of Reported Results: 509 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
117

0

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laboratory number

 mass fraction (ug/g)

1,6,7-trimethylnaphthalene QA10OIL01

Assigned mean = 320 ug/g s = 119 ug/g 95% CI = 58 ug/g Assigned median = 306 ug/g 

Reported Results: 16 Quantitative Results: 16 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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200

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300

350

400

450

laboratory number

mass fraction (ug/g)

1,6,7-trimethylnaphthalene SRM 1582

Target Value = no target ug/g 

Reported Results: 16 Quantitative Results: 16 Median of Reported Results: 250 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
118

0

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350

laboratory number

 mass fraction (ug/g)

1-methylphenanthrene QA10OIL01

Assigned mean = 166 ug/g s = 43 ug/g 95% CI = 18 ug/g Assigned median = 173 ug/g 

Reported Results: 23 Quantitative Results: 23 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

50

100

150

200

250

300

350

laboratory number

mass fraction (ug/g)

1-methylphenanthrene SRM 1582

Target Value = no target ug/g 

Reported Results: 23 Quantitative Results: 23 Median of Reported Results: 83.3 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
119

0

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laboratory number

 mass fraction (ug/g)

C1-decalins QA10OIL01

Assigned mean = 1235 ug/g s = 390 ug/g 95% CI = 312 ug/g Assigned median = 1042 ug/g 

Reported Results: 9 Quantitative Results: 6 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

200

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600

800

1000

1200

1400

1600

laboratory number

mass fraction (ug/g)

C1-decalins SRM 1582

Target Value = no target ug/g 

Reported Results: 9 Quantitative Results: 7 Median of Reported Results: 859 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
120

0

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2500

laboratory number

 mass fraction (ug/g)

C2-decalins QA10OIL01

Assigned mean = 1221 ug/g s = 448 ug/g 95% CI = 359 ug/g Assigned median = 1064 ug/g 

Reported Results: 9 Quantitative Results: 6 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

500

1000

1500

2000

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3000

laboratory number

mass fraction (ug/g)

C2-decalins SRM 1582

Target Value = no target ug/g 

Reported Results: 9 Quantitative Results: 6 Median of Reported Results: 1533 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
121

0

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1600

laboratory number

 mass fraction (ug/g)

C3-decalins QA10OIL01

Assigned mean = 833 ug/g s = 449 ug/g 95% CI = 359 ug/g Assigned median = 757 ug/g 

Reported Results: 9 Quantitative Results: 6 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

500

1000

1500

2000

2500

laboratory number

mass fraction (ug/g)

C3-decalins SRM 1582

Target Value = no target ug/g 

Reported Results: 9 Quantitative Results: 6 Median of Reported Results: 1457 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
122

0

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600

800

1000

1200

1400

laboratory number

 mass fraction (ug/g)

C4-decalins QA10OIL01

Assigned mean = 696 ug/g s = 289 ug/g 95% CI = 231 ug/g Assigned median = 678 ug/g 

Reported Results: 9 Quantitative Results: 6 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

200

400

600

800

1000

1200

1400

1600

laboratory number

mass fraction (ug/g)

C4-decalins SRM 1582

Target Value = no target ug/g 

Reported Results: 9 Quantitative Results: 6 Median of Reported Results: 860 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
123

0

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3000

3500

4000

laboratory number

 mass fraction (ug/g)

C1-naphthalenes QA10OIL01

Assigned mean = 2115 ug/g s = 544 ug/g 95% CI = 233 ug/g Assigned median = 1833 ug/g 

Reported Results: 21 Quantitative Results: 21 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

200

400

600

800

1000

1200

laboratory number

mass fraction (ug/g)

C1-naphthalenes SRM 1582

Target Value = no target ug/g 

Reported Results: 21 Quantitative Results: 21 Median of Reported Results: 615 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
124

0

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1500

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2500

3000

3500

4000

4500

laboratory number

 mass fraction (ug/g)

C2-naphthalenes QA10OIL01

Assigned mean = 2409 ug/g s = 830 ug/g 95% CI = 339 ug/g Assigned median = 2173 ug/g 

Reported Results: 23 Quantitative Results: 23 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

500

1000

1500

2000

2500

laboratory number

mass fraction (ug/g)

C2-naphthalenes SRM 1582

Target Value = no target ug/g 

Reported Results: 23 Quantitative Results: 23 Median of Reported Results: 1080 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
125

0

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3000

3500

laboratory number

 mass fraction (ug/g)

C3-naphthalenes QA10OIL01

Assigned mean = 1571 ug/g s = 604 ug/g 95% CI = 252 ug/g Assigned median = 1383 ug/g 

Reported Results: 23 Quantitative Results: 23 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

500

1000

1500

2000

2500

laboratory number

mass fraction (ug/g)

C3-naphthalenes SRM 1582

Target Value = no target ug/g 

Reported Results: 23 Quantitative Results: 23 Median of Reported Results: 1062 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
126

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1800

laboratory number

 mass fraction (ug/g)

C4-naphthalenes QA10OIL01

Assigned mean = 712 ug/g s = 308 ug/g 95% CI = 126 ug/g Assigned median = 698 ug/g 

Reported Results: 23 Quantitative Results: 23 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

200

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600

800

1000

1200

1400

1600

1800

laboratory number

mass fraction (ug/g)

C4-naphthalenes SRM 1582

Target Value = no target ug/g 

Reported Results: 23 Quantitative Results: 23 Median of Reported Results: 740 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
127

0.0

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70.0

80.0

laboratory number

 mass fraction (ug/g)

C1-benzothiophenes QA10OIL01

Assigned mean = 40.1 ug/g s = 17.8 ug/g 95% CI = 13.2 ug/g Assigned median = 32.3 ug/g 

Reported Results: 9 Quantitative Results: 8 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

laboratory number

mass fraction (ug/g)

C1-benzothiophenes SRM 1582

Target Value = no target ug/g 

Reported Results: 9 Quantitative Results: 8 Median of Reported Results: 47.9 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
128

0.0

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20.0

30.0

40.0

50.0

60.0

70.0

laboratory number

 mass fraction (ug/g)

C2-benzothiophenes QA10OIL01

Assigned mean = 37.5 ug/g s = 13.8 ug/g 95% CI = 10.2 ug/g Assigned median = 35.6 ug/g 

Reported Results: 9 Quantitative Results: 8 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

50

100

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200

250

300

350

laboratory number

mass fraction (ug/g)

C2-benzothiophenes SRM 1582

Target Value = no target ug/g 

Reported Results: 9 Quantitative Results: 9 Median of Reported Results: 96.2 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
129

0

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400

laboratory number

 mass fraction (ug/g)

C3-benzothiophenes QA10OIL01

Assigned value = No assigned value 

Reported Results: 9 Quantitative Results: 8 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

200

400

600

800

1000

1200

1400

laboratory number

mass fraction (ug/g)

C3-benzothiophenes SRM 1582

Target Value = no target ug/g 

Reported Results: 9 Quantitative Results: 8 Median of Reported Results: not calculated

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
130

0.0

10.0

20.0

30.0

40.0

50.0

60.0

laboratory number

 mass fraction (ug/g)

C4-benzothiophenes QA10OIL01

Assigned mean = 28.8 ug/g s = 3.5 ug/g 95% CI = 2.8 ug/g Assigned median = 30.3 ug/g 

Reported Results: 8 Quantitative Results: 6 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

20

40

60

80

100

120

140

160

180

laboratory number

mass fraction (ug/g)

C4-benzothiophenes SRM 1582

Target Value = no target ug/g 

Reported Results: 8 Quantitative Results: 6 Median of Reported Results: 142 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
131

0

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700

800

900

laboratory number

 mass fraction (ug/g)

C1-fluorenes QA10OIL01

Assigned mean = 332 ug/g s = 146 ug/g 95% CI = 60 ug/g Assigned median = 299 ug/g 

Reported Results: 23 Quantitative Results: 23 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

50

100

150

200

250

300

350

400

450

laboratory number

mass fraction (ug/g)

C1-fluorenes SRM 1582

Target Value = no target ug/g 

Reported Results: 23 Quantitative Results: 22 Median of Reported Results: 115 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
132

0

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700

800

laboratory number

 mass fraction (ug/g)

C2-fluorenes QA10OIL01

Assigned mean = 384 ug/g s = 64 ug/g 95% CI = 28 ug/g Assigned median = 380 ug/g 

Reported Results: 22 Quantitative Results: 22 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

100

200

300

400

500

600

laboratory number

mass fraction (ug/g)

C2-fluorenes SRM 1582

Target Value = no target ug/g 

Reported Results: 22 Quantitative Results: 21 Median of Reported Results: 229 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
133

0

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400

500

600

laboratory number

 mass fraction (ug/g)

C3-fluorenes QA10OIL01

Assigned mean = 280 ug/g s = 87 ug/g 95% CI = 37 ug/g Assigned median = 267 ug/g 

Reported Results: 23 Quantitative Results: 23 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

50

100

150

200

250

300

350

400

laboratory number

mass fraction (ug/g)

C3-fluorenes SRM 1582

Target Value = no target ug/g 

Reported Results: 22 Quantitative Results: 22 Median of Reported Results: 230 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
134

0

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1400

laboratory number

 mass fraction (ug/g)

C1-phenanthrenes/anthracenes QA10OIL01

Assigned mean = 731 ug/g s = 204 ug/g 95% CI = 83 ug/g Assigned median = 667 ug/g 

Reported Results: 23 Quantitative Results: 23 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

100

200

300

400

500

600

700

800

laboratory number

mass fraction (ug/g)

C1-phenanthrenes/anthracenes SRM 1582

Target Value = no target ug/g 

Reported Results: 23 Quantitative Results: 23 Median of Reported Results: 347 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
135

0

200

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600

800

1000

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1400

laboratory number

 mass fraction (ug/g)

C2-phenanthrenes/anthracenes QA10OIL01

Assigned mean = 670 ug/g s = 146 ug/g 95% CI = 61 ug/g Assigned median = 628 ug/g 

Reported Results: 23 Quantitative Results: 23 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

100

200

300

400

500

600

700

800

laboratory number

mass fraction (ug/g)

C2-phenanthrenes/anthracenes SRM 1582

Target Value = no target ug/g 

Reported Results: 23 Quantitative Results: 23 Median of Reported Results: 480 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
136

0

200

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800

1000

1200

laboratory number

 mass fraction (ug/g)

C3-phenanthrenes/anthracenes QA10OIL01

Assigned mean = 411 ug/g s = 109 ug/g 95% CI = 47 ug/g Assigned median = 398 ug/g 

Reported Results: 23 Quantitative Results: 22 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

200

400

600

800

1000

1200

laboratory number

mass fraction (ug/g)

C3-phenanthrenes/anthracenes SRM 1582

Target Value = no target ug/g 

Reported Results: 23 Quantitative Results: 22 Median of Reported Results: 427 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
137

0

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150

200

250

300

350

400

laboratory number

 mass fraction (ug/g)

C4-phenanthrenes/anthracenes QA10OIL01

Assigned mean = 195 ug/g s = 69 ug/g 95% CI = 30 ug/g Assigned median = 197 ug/g 

Reported Results: 23 Quantitative Results: 22 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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700

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900

laboratory number

mass fraction (ug/g)

C4-phenanthrenes/anthracenes SRM 1582

Target Value = no target ug/g 

Reported Results: 23 Quantitative Results: 21 Median of Reported Results: 235 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
138

0

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laboratory number

 mass fraction (ug/g)

C1-dibenzothiophenes QA10OIL01

Assigned mean = 125 ug/g s = 36 ug/g 95% CI = 15 ug/g Assigned median = 132 ug/g 

Reported Results: 23 Quantitative Results: 23 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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laboratory number

mass fraction (ug/g)

C1-dibenzothiophenes SRM 1582

Target Value = no target ug/g 

Reported Results: 23 Quantitative Results: 22 Median of Reported Results: 124 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
139

0

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laboratory number

 mass fraction (ug/g)

C2-dibenzothiophenes QA10OIL01

Assigned mean = 159 ug/g s = 47 ug/g 95% CI = 20 ug/g Assigned median = 156 ug/g 

Reported Results: 23 Quantitative Results: 23 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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150

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laboratory number

mass fraction (ug/g)

C2-dibenzothiophenes SRM 1582

Target Value = no target ug/g 

Reported Results: 23 Quantitative Results: 22 Median of Reported Results: 190 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
140

0

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laboratory number

 mass fraction (ug/g)

C3-dibenzothiophenes QA10OIL01

Assigned mean = 117 ug/g s = 26 ug/g 95% CI = 12 ug/g Assigned median = 114 ug/g 

Reported Results: 22 Quantitative Results: 20 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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100

150

200

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300

laboratory number

mass fraction (ug/g)

C3-dibenzothiophenes SRM 1582

Target Value = no target ug/g 

Reported Results: 22 Quantitative Results: 20 Median of Reported Results: 165 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
141

0

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laboratory number

 mass fraction (ug/g)

C4-dibenzothiophenes QA10OIL01

Assigned mean = 57.4 ug/g s = 20.2 ug/g 95% CI = 9.3 ug/g Assigned median = 56.0 ug/g 

Reported Results: 20 Quantitative Results: 18 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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laboratory number

mass fraction (ug/g)

C4-dibenzothiophenes SRM 1582

Target Value = no target ug/g 

Reported Results: 20 Quantitative Results: 17 Median of Reported Results: 101 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
142

0

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laboratory number

 mass fraction (ug/g)

C1-fluoranthenes/pyrenes QA10OIL01

Assigned mean = 72.8 ug/g s = 15.9 ug/g 95% CI = 7.0 ug/g Assigned median = 67.2 ug/g 

Reported Results: 23 Quantitative Results: 22 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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laboratory number

mass fraction (ug/g)

C1-fluoranthenes/pyrenes SRM 1582

Target Value = no target ug/g 

Reported Results: 23 Quantitative Results: 22 Median of Reported Results: 54.4 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
143

0

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laboratory number

 mass fraction (ug/g)

C2-fluoranthenes/pyrenes QA10OIL01

Assigned mean = 130 ug/g s = 33 ug/g 95% CI = 15 ug/g Assigned median = 125 ug/g 

Reported Results: 21 Quantitative Results: 19 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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100

150

200

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laboratory number

mass fraction (ug/g)

C2-fluoranthenes/pyrenes SRM 1582

Target Value = no target ug/g 

Reported Results: 20 Quantitative Results: 18 Median of Reported Results: 98.0 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
144

0

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laboratory number

 mass fraction (ug/g)

C3-fluoranthenes/pyrenes QA10OIL01

Assigned mean = 126 ug/g s = 33 ug/g 95% CI = 14 ug/g Assigned median = 122 ug/g 

Reported Results: 22 Quantitative Results: 21 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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laboratory number

mass fraction (ug/g)

C3-fluoranthenes/pyrenes SRM 1582

Target Value = no target ug/g 

Reported Results: 21 Quantitative Results: 19 Median of Reported Results: 118 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
145

0

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laboratory number

 mass fraction (ug/g)

C4-fluoranthenes/pyrenes QA10OIL01

Assigned mean = 95.0 ug/g s = 32.5 ug/g 95% CI = 18.4 ug/g Assigned median = 87.0 ug/g 

Reported Results: 13 Quantitative Results: 13 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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150

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250

laboratory number

mass fraction (ug/g)

C4-fluoranthenes/pyrenes SRM 1582

Target Value = no target ug/g 

Reported Results: 13 Quantitative Results: 12 Median of Reported Results: 110 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
146

0

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laboratory number

 mass fraction (ug/g)

C1-naphthobenzothiophenes QA10OIL01

Assigned mean = 54.8 ug/g s = 18.4 ug/g 95% CI = 12.7 ug/g Assigned median = 57.1 ug/g 

Reported Results: 9 Quantitative Results: 9 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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40

50

60

70

80

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100

laboratory number

mass fraction (ug/g)

C1-naphthobenzothiophenes SRM 1582

Target Value = no target ug/g 

Reported Results: 9 Quantitative Results: 8 Median of Reported Results: 62.4 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
147

0

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120

140

laboratory number

 mass fraction (ug/g)

C2-naphthobenzothiophenes QA10OIL01

Assigned mean = 68.4 ug/g s = 22.7 ug/g 95% CI = 15.7 ug/g Assigned median = 69.7 ug/g 

Reported Results: 9 Quantitative Results: 8 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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60

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laboratory number

mass fraction (ug/g)

C2-naphthobenzothiophenes SRM 1582

Target Value = no target ug/g 

Reported Results: 9 Quantitative Results: 8 Median of Reported Results: 84.2 ug/g 

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
148

0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

80.0

90.0

laboratory number

 mass fraction (ug/g)

C3-naphthobenzothiophenes QA10OIL01

Assigned mean = 47.0 ug/g s = 14.0 ug/g 95% CI = 9.7 ug/g Assigned median = 47.8 ug/g 

Reported Results: 9 Quantitative Results: 8 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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40

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60

70

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laboratory number

mass fraction (ug/g)

C3-naphthobenzothiophenes SRM 1582

Target Value = no target ug/g 

Reported Results: 9 Quantitative Results: 7 Median of Reported Results: 79.2 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
149

0.0

10.0

20.0

30.0

40.0

50.0

60.0

laboratory number

 mass fraction (ug/g)

C4-naphthobenzothiophenes QA10OIL01

Assigned mean = 28.9 ug/g s = 10.3 ug/g 95% CI = 7.6 ug/g Assigned median = 30.7 ug/g 

Reported Results: 9 Quantitative Results: 7 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

20

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60

80

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120

laboratory number

mass fraction (ug/g)

C4-naphthobenzothiophenes SRM 1582

Target Value = no target ug/g 

Reported Results: 9 Quantitative Results: 7 Median of Reported Results: 52.3 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
150

0

50

100

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300

laboratory number

 mass fraction (ug/g)

C1-chrysenes QA10OIL01

Assigned mean = 108 ug/g s = 16 ug/g 95% CI = 7 ug/g Assigned median = 108 ug/g 

Reported Results: 22 Quantitative Results: 22 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

80.0

90.0

laboratory number

mass fraction (ug/g)

C1-chrysenes SRM 1582

Target Value = no target ug/g 

Reported Results: 21 Quantitative Results: 19 Median of Reported Results: 54.0 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
151

0

50

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150

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300

350

400

laboratory number

 mass fraction (ug/g)

C2-chrysenes QA10OIL01

Assigned mean = 130 ug/g s = 19 ug/g 95% CI = 9 ug/g Assigned median = 128 ug/g 

Reported Results: 22 Quantitative Results: 21 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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140

160

laboratory number

mass fraction (ug/g)

C2-chrysenes SRM 1582

Target Value = no target ug/g 

Reported Results: 21 Quantitative Results: 19 Median of Reported Results: 102 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
152

0

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180

laboratory number

 mass fraction (ug/g)

C3-chrysenes QA10OIL01

Assigned mean = 91.3 ug/g s = 24.7 ug/g 95% CI = 11.4 ug/g Assigned median = 92.7 ug/g 

Reported Results: 21 Quantitative Results: 19 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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laboratory number

mass fraction (ug/g)

C3-chrysenes SRM 1582

Target Value = no target ug/g 

Reported Results: 20 Quantitative Results: 18 Median of Reported Results: 91.5 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
153

0

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140

laboratory number

 mass fraction (ug/g)

C4-chrysenes QA10OIL01

Assigned mean = 65.4 ug/g s = 15.8 ug/g 95% CI = 8.6 ug/g Assigned median = 70.9 ug/g 

Reported Results: 19 Quantitative Results: 15 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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laboratory number

mass fraction (ug/g)

C4-chrysenes SRM 1582

Target Value = no target ug/g 

Reported Results: 18 Quantitative Results: 15 Median of Reported Results: 78.1 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
154

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

laboratory number

 mass fraction (ug/g)

Carbazole QA10OIL01

Assigned mean = 8.19 ug/g s = 2.48 ug/g 95% CI = 2.43 ug/g Assigned median = 7.68 ug/g 

Reported Results: 7 Quantitative Results: 4 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

laboratory number

mass fraction (ug/g)

Carbazole SRM 1582

Reference Value = 3.4 ug/g ; 95% CI 0.3 ug/g

Reported Results: 6 Quantitative Results: 4 Median of Reported Results: 8.49 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
155

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

laboratory number

 mass fraction (ug/g)

18a(H)-22,29,30-Trisnorhopane QA10OIL01

Assigned mean = 6.97 ug/g s = 1.35 ug/g 95% CI = 0.94 ug/g Assigned median = 6.93 ug/g 

Reported Results: 11 Quantitative Results: 8 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

45.0

laboratory number

mass fraction (ug/g)

18a(H)-22,29,30-Trisnorhopane SRM 1582

Target Value = no target ug/g 

Reported Results: 10 Quantitative Results: 10 Median of Reported Results: 21.8 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
156

0.0

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10.0

15.0

20.0

25.0

30.0

laboratory number

 mass fraction (ug/g)

17a(H)-22,29,30-Trisnorhopane QA10OIL01

Assigned mean = 7.07 ug/g s = 2.16 ug/g 95% CI = 1.34 ug/g Assigned median = 6.98 ug/g 

Reported Results: 15 Quantitative Results: 12 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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laboratory number

mass fraction (ug/g)

17a(H)-22,29,30-Trisnorhopane SRM 1582

Target Value = no target ug/g 

Reported Results: 14 Quantitative Results: 13 Median of Reported Results: 47.0 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
157

0.0

10.0

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30.0

40.0

50.0

60.0

70.0

laboratory number

 mass fraction (ug/g)

17α(H),21β(H)-30-Norhopane QA10OIL01

Assigned mean = 17.1 ug/g s = 4.3 ug/g 95% CI = 2.8 ug/g Assigned median = 15.9 ug/g 

Reported Results: 14 Quantitative Results: 11 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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laboratory number

mass fraction (ug/g)

17α(H),21β(H)-30-Norhopane SRM 1582

Target Value = no target ug/g 

Reported Results: 13 Quantitative Results: 13 Median of Reported Results: 139 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
158

0.0

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

18.0

laboratory number

 mass fraction (ug/g)

18a(H)-30-Norneohopane QA10OIL01

Assigned mean = 6.46 ug/g s = 2.17 ug/g 95% CI = 1.60 ug/g Assigned median = 5.58 ug/g 

Reported Results: 11 Quantitative Results: 8 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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laboratory number

mass fraction (ug/g)

18a(H)-30-Norneohopane SRM 1582

Target Value = no target ug/g 

Reported Results: 10 Quantitative Results: 10 Median of Reported Results: 37.1 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
159

0.00

1.00

2.00

3.00

4.00

5.00

6.00

7.00

8.00

laboratory number

 mass fraction (ug/g)

17a(H)-Diahopane QA10OIL01

Assigned mean = 4.19 ug/g s = 1.33 ug/g 95% CI = 0.98 ug/g Assigned median = 4.47 ug/g 

Reported Results: 10 Quantitative Results: 7 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0.0

5.0

10.0

15.0

20.0

25.0

30.0

laboratory number

mass fraction (ug/g)

17a(H)-Diahopane SRM 1582

Target Value = no target ug/g 

Reported Results: 9 Quantitative Results: 2 Median of Reported Results: not calculated

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
160

0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

80.0

90.0

laboratory number

 mass fraction (ug/g)

17α(H),21β(H)-Hopane QA10OIL01

Assigned mean = 43.8 ug/g s = 9.2 ug/g 95% CI = 4.8 ug/g Assigned median = 43.6 ug/g 

Reported Results: 16 Quantitative Results: 14 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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laboratory number

mass fraction (ug/g)

17α(H),21β(H)-Hopane SRM 1582

Target Value = no target ug/g 

Reported Results: 15 Quantitative Results: 15 Median of Reported Results: 256 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
161

0.0

5.0

10.0

15.0

20.0

25.0

laboratory number

 mass fraction (ug/g)

17α(H),21β(H)-22R-Homohopane QA10OIL01

Assigned mean = 12.8 ug/g s = 2.8 ug/g 95% CI = 1.7 ug/g Assigned median = 12.1 ug/g 

Reported Results: 12 Quantitative Results: 11 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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laboratory number

mass fraction (ug/g)

17α(H),21β(H)-22R-Homohopane SRM 1582

Target Value = no target ug/g 

Reported Results: 12 Quantitative Results: 12 Median of Reported Results: 73.3 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
162

0.0

5.0

10.0

15.0

20.0

25.0

30.0

laboratory number

 mass fraction (ug/g)

17α(H),21β(H)-22S-Homohopane QA10OIL01

Assigned mean = 15.7 ug/g s = 3.4 ug/g 95% CI = 2.0 ug/g Assigned median = 15.4 ug/g 

Reported Results: 12 Quantitative Results: 11 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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160

laboratory number

mass fraction (ug/g)

17α(H),21β(H)-22S-Homohopane SRM 1582

Target Value = no target ug/g 

Reported Results: 12 Quantitative Results: 12 Median of Reported Results: 81.7 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
163

0.0

10.0

20.0

30.0

40.0

50.0

60.0

70.0

80.0

90.0

laboratory number

 mass fraction (ug/g)

13b(H)17a(H)-Diacholestane 20S QA10OIL01

Assigned mean = 42.3 ug/g s = 7.2 ug/g 95% CI = 5.3 ug/g Assigned median = 41.2 ug/g 

Reported Results: 10 Quantitative Results: 8 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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40

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laboratory number

mass fraction (ug/g)

13b(H)17a(H)-Diacholestane 20S SRM 1582

Target Value = no target ug/g 

Reported Results: 9 Quantitative Results: 9 Median of Reported Results: 95.7 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
164

0

10

20

30

40

50

60

70

80

90

100

laboratory number

 mass fraction (ug/g)

5a(H),14a(H),17a(H)-Cholestane 20S QA10OIL01

Assigned mean = 46.5 ug/g s = 25.2 ug/g 95% CI = 17.4 ug/g Assigned median = 46.9 ug/g 

Reported Results: 10 Quantitative Results: 8 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

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laboratory number

mass fraction (ug/g)

5a(H),14a(H),17a(H)-Cholestane 20S SRM 1582

Target Value = no target ug/g 

Reported Results: 9 Quantitative Results: 9 Median of Reported Results: 233 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
165

0

10

20

30

40

50

60

70

80

90

100

laboratory number

 mass fraction (ug/g)

5a(H),14a(H),17a(H)-Cholestane 20R QA10OIL01

Assigned mean = 44.4 ug/g s = 23.9 ug/g 95% CI = 13.0 ug/g Assigned median = 50.5 ug/g 

Reported Results: 15 Quantitative Results: 13 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

100

200

300

400

500

600

laboratory number

mass fraction (ug/g)

5a(H),14a(H),17a(H)-Cholestane 20R SRM 1582

Target Value = no target ug/g 

Reported Results: 14 Quantitative Results: 13 Median of Reported Results: 344 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
166

0.0

10.0

20.0

30.0

40.0

50.0

60.0

laboratory number

 mass fraction (ug/g)

5a(H),14a(H),17a(H)-24-Ethylcholestane 20S QA10OIL01

Assigned mean = 26.1 ug/g s = 11.2 ug/g 95% CI = 7.7 ug/g Assigned median = 26.2 ug/g 

Reported Results: 10 Quantitative Results: 8 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

50

100

150

200

250

300

350

400

laboratory number

mass fraction (ug/g)

5a(H),14a(H),17a(H)-24-Ethylcholestane 20S SRM 1582

Target Value = no target ug/g 

Reported Results: 11 Quantitative Results: 9 Median of Reported Results: 223 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
167

0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

45.0

laboratory number

 mass fraction (ug/g)

5a(H),14a(H),17a(H)-24-Ethylcholestane 20R QA10OIL01

Assigned mean = 23.6 ug/g s = 9.9 ug/g 95% CI = 6.1 ug/g Assigned median = 19.9 ug/g 

Reported Results: 13 Quantitative Results: 11 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

50

100

150

200

250

300

350

400

450

500

laboratory number

mass fraction (ug/g)

5a(H),14a(H),17a(H)-24-Ethylcholestane 20R SRM 1582

Target Value = no target ug/g 

Reported Results: 13 Quantitative Results: 12 Median of Reported Results: 255 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
168

0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

45.0

50.0

laboratory number

 mass fraction (ug/g)

5a(H),14b(H),17b(H)-Cholestane 20R QA10OIL01

Assigned mean = 25.5 ug/g s = 8.1 ug/g 95% CI = 4.8 ug/g Assigned median = 24.6 ug/g 

Reported Results: 13 Quantitative Results: 11 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

50

100

150

200

250

300

laboratory number

mass fraction (ug/g)

5a(H),14b(H),17b(H)-Cholestane 20R SRM 1582

Target Value = no target ug/g 

Reported Results: 12 Quantitative Results: 12 Median of Reported Results: 179 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
169

0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

45.0

50.0

laboratory number

 mass fraction (ug/g)

5a(H),14b(H),17b(H)-Cholestane 20S QA10OIL01

Assigned mean = 24.9 ug/g s = 9.7 ug/g 95% CI = 6.4 ug/g Assigned median = 22.3 ug/g 

Reported Results: 11 Quantitative Results: 9 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

50

100

150

200

250

300

laboratory number

mass fraction (ug/g)

5a(H),14b(H),17b(H)-Cholestane 20S SRM 1582

Target Value = no target ug/g 

Reported Results: 10 Quantitative Results: 10 Median of Reported Results: 174 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
170

0.0

10.0

20.0

30.0

40.0

50.0

60.0

laboratory number

 mass fraction (ug/g)

5a(H),14b(H),17b(H)-24-Ethylcholestane 20R QA10OIL01

Assigned mean = 26.0 ug/g s = 9.7 ug/g 95% CI = 5.5 ug/g Assigned median = 24.8 ug/g 

Reported Results: 14 Quantitative Results: 12 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

50

100

150

200

250

300

laboratory number

mass fraction (ug/g)

5a(H),14b(H),17b(H)-24-Ethylcholestane 20R SRM 1582

Target Value = no target ug/g 

Reported Results: 13 Quantitative Results: 13 Median of Reported Results: 139 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
171

0.0

5.0

10.0

15.0

20.0

25.0

30.0

35.0

40.0

45.0

50.0

laboratory number

 mass fraction (ug/g)

5a(H),14b(H),17b(H)-24-Ethylcholestane 20S QA10OIL01

Assigned mean = 25.4 ug/g s = 8.4 ug/g 95% CI = 5.5 ug/g Assigned median = 23.1 ug/g 

Reported Results: 12 Quantitative Results: 9 

Solid line : exercise assigned value (EAV); dotted line: z=±1 (25% from EAV); dotted/dashed 

line: z=±2 (50% from EAV); dashed line: z=±3 (75% from EAV)

0

20

40

60

80

100

120

140

160

180

200

laboratory number

mass fraction (ug/g)

5a(H),14b(H),17b(H)-24-Ethylcholestane 20S SRM 1582

Target Value = no target ug/g 

Reported Results: 11 Quantitative Results: 10 Median of Reported Results: 115 ug/g

Solid line: value from Certificate of Analysis ; dotted line: 95% confidence limits; dashed line: 30% 

from 95% confidence limits
172

Appendix E

Participants in the Sediment Interlaboratory Study QA10OIL01 in 

Alphabetical Order by Organization

Alpha Analytical, Inc.

320 Forbes Blvd

Mansfield, MA 02048

Elizabeth Porta

ALS Environmental Division

5424 97th St. NW

Edmonton, AB T6E 5C1 CANADA

Deib Birkholz / Sarah Stilson

Battelle Analytical & Environmental Chemistry Laboratory

397 Washington Street

Duxbury, MA 02332

Kerylynn Krahforst

Columbia Analytical Services (CAS)

9143 Philips Highway, Suite 200

Jacksonville, FL 32256

Joe Wiegel / Karenya Fedele

Columbia Analytical Services (CAS)

1 Mustard Street, Ste. 250

Rochester, NY 14609

Mike Perry

Columbia Analytical Services (CAS)

1317 South 13th Avenue

Kelso, WA 98626

Greg Salata / Julie Gish

Florida International University

Department of Chemistry and Biochemistry and Southeast Environmental Research 

Center (SERC)

3000 NE 151 Street

Biscayne Bay Campus

Marine Science Building MSB 356

North Miami, FL 33181

Piero Gardinali
173

NIST

Hollings Marine Laboratory

331 Fort Johnson Road

Charleston, SC 29412

John Kucklick

NIST

100 Bureau Drive, MS 8392

Gaithersburg, MD 20899-8392

Michele Schantz

New York State Department of Health

Biggs Laboratory Room D-539

Wadsworth Center

Empire State Plaza

Albany, NY 12237

Kenneth Aldous / Shannon Shaver

NOAA/NCCOS/NOS

Chemical Contaminants Research Program

Center for Coastal Environmental Health and Biomolecular Research

331 Fort Johnson Road

Charleston, SC 29412

Ed Wirth

NOAA/NMFS/Alaska Fisheries Science Center

Auke Bay Laboratories

17109 Pt Lena Loop Road

Juneau, AK 99801

Mark Carls / Marie Larsen

NOAA/NMFS/NW Fisheries Science Center

2725 Montlake Blvd. East

Seattle, WA 98112-2097

Jennie Bolton / Catherine Sloan / Gina Yitalo

Pace Analytical Services, Inc. Minneapolis

1700 Elm Street

Minneapolis, MN 55414

Charity Nowlan

RJ Lee Group, Inc

350 Hochberg Road

Monroeville, PA 15146

Keith Rickabaugh / Alan Levine / Steven Schlager
174

RJ Lee Group, Inc. 

Center for Laboratory Sciences

2710 North 20th Avenue

Pasco, WA 99301

Yemane Yohannes

TDI/B&B Laboratories, Inc

1902 Pinon

College Station, TX 77845

Juan Ramirez

TestAmerica Laboratories

900 Lakeside Drive

Mobile, AL 36693

Charles Newton / Eron Schellinger

TestAmerica Laboratories

880 Riverside Parkway 

West Sacramento, CA 95605

Karla Buechler / Steven Rogers

TestAmerica Laboratories

2417 Bond Street

University Park, IL 60484

Michael Healy / Terese Preston / Gary Rynker / Garth Swaney

TestAmerica Laboratories

301 Alpha Drive

RIDC Park

Pittsburgh, PA 15238

Larry Matko / Sharon Bacha

TestAmerica Laboratories

5815 Middlebrook Pike

Knoxville, TN 37921

Tom Yoder/ Bruce Wagner

TestAmerica Laboratories

30 Community Drive

Suite 11

South Burlington, VT 05403

Bryce Stearns
175

TestAmerica Laboratories

5755 8th Street East

Tacoma, WA 98424

Kathy Kreps / Coleen McKean

Texas A&M University

Geochemical and Environmental Research Group

833 Graham Road

College Station, TX 77845

Jóse Sericano / Terry Wade

University of Iowa

State Hygienic Laboratory

#H 101 OH, 102 Oakdale Campus

Iowa City, Iowa 52242-5002

Terry Cain and Michael Wichman