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Hylebos Waterway Fish Injury Studies

Individual Data and Quality Assurance Results

CASE NARRATIVE

Reproductive Toxicology in Flatfish

(Revised 1/22/97)

Rapid PCB Analysis of Livers (Table 1)

Calibrations

The calibration data used to quantitate the analytes (Tables 1J and 1K) met thecontinuing

calibration criteria detailed in the “Hylebos Waterway Fish InjuryQuality Assurance Plan,

4/25/95” (QAP).

Method Blank Analysis

The criteria in the QAP for method blanks (Tables 1E and 1F) were met (nomore than 4

analytes to exceed 4 X the MDL).

Surrogate Recoveries

Surrogate recoveries for samples analyzed by HPLC/PDA for dioxin-like PCBs and other

selected PCBs and pesticides (Tables 1A, 1D and 1G) were within the guidelines detailed

in the QA Plan (60-125% recovery).

Control Material Analyses

An aliquot of NIST whale blubber control materW (QC91-WB1) was analyzed with each

of the sample sets and the results (Tables 1E and 1F) met the criteria in the QA plan

(concentrations of >70% of these selected analytes, [PCBs 105, 118,138,153,156,180,

p,p’-DDE (at 266 nm), o,p’-DDD, p,p’-DDD, pp’-DDT, hexachlorobenzene] were within

±50% of the published reference values). Non-certified values for the other analytes in

the NIST control material are also shown in the tables.

Sample Duplicates

Eleven samples were analyzed in duplicate (Tables 1H and 1I) and the criteria(RSD ≤

50%) in the QAP were met (QA plan, Table 4).
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Reanalyses

The method blank in one sample set (HY015 through HY028) contained a large

interfering peak and therefore the English sole liver samples from that set were

re-extracted, and reanalyzed as samples HY154 through HY161. The following samples

from that set were not re-extracted because < 0.20 g of sample remained to be extracted

and thus these samples were not analyzed: 94.35281, 94.3530, 94.3531, 94.3532, 94.3535

and 94.3544. One sample (94.3577) was inadvertently not analyzed with the other

samples from the same sample set (HY043 through HY056). Therefore, the QA samples

(method blank, NIST whale blubber control material and samples duplicates) from this

sample set were reanalyzed with the sample 943577.

Table Revision (01/22/97)

Table 1B-p2. For sample 94.3600, the concentration value of.CB189 was inadvertently

missed and was reported as <0.16 ng/g, wet weight. The correct value for CB189 is 1.8

ng/g wet weight.
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Hylebos Waterway Damage Assessment Project/1994-1995

Rapid Analyses for PCBs

Table I Notes

The concentrations of analytes were calculated using 1,7,8-trichlorodibenzo-p-dioxin as the

surrogate standard.

The “less than” symbol (<) indicates that the analyte was not detected in concentrations

above the stated value.

Analyte concentrations were determined by high-performance liquid chromatography with

photodiode array detection WLC/PDA) at 220 nm except p,p’-DDE concentrations; this

analyte was analyzed by BrLC/PDA at 266 nm.

Analyte concentrations are rounded to two significant figures.

The percent recoveries of the surrogate standard were calculated using 1,2,3,4-

tetrachlorodibenzo-p-dioxin added after the acidic silica gel cleanup step.

Extract number designation was used for internal lab use and identification only. Field

number and site name represent Hylebos Damage Assessment official sample

identification designations.

The sample weights used to calculate concentrations for the method blanks are the mean

sample weights calculated for the field samples in the corresponding sample set.
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