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Hylebos Waterway Fish Injury Studies

Individual Data and Quality Assurance Results

CASE NARRATIVES

Reproductive Toxicology in Flatfish

Semivolatile Organics (Tables 1, 2)

Calibrations

The calibration data used to quantitate the analytes met the initial (Tables 1J, 2G) and

continuing (Tables 1K, 1L and 2H) calibration criteria detailed in the

“Commencement Bay Quality Assurance Plan, 12/95” (QAP).

Method Blank Analysis

Method blanks were analyzed for CHs (Tables 1E, IF) and AHs (Table 2D). The

criteria in the QAP for method blanks were met (no more than 4 analytes to exceed 3

X the MDLs listed in Tables 1M, 2I).

Surrogate Recoveries

Surrogate recoveries for samples analyzed by GC/MS for AHs and by GC/ECD for

PCBs, DDTs and pesticides (Tables 1A, 1D, 2A, and 2C) were within the guidelines

detailed in the QA Plan (50-125% recovery).

SRM Analyses

An aliquot of NIST tissue SRM 1974a was analyzed with each of the sample sets, and

the results (Tables 1E, 1F, and 2D) met the criteria in the QA plan (>70% of

concentrations for the certified analytes that were present in NIST SRM 1974a in

concentrations greater than 10 times the MDL were within 35% of either end of the

NIST values. Noncertified values for the other analytes in the SRM and

concentrations from samples of the SRM analyzed previously are also shown in the

tables.

Sample Duplicates

One sample was analyzed in duplicate (Tables I H, 11, and 2F) and the criteria in the

QAP were met (QA plan, Table 6.2).
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Reanalyses

There is no plan to reanalyze any samples.

GUMS Confiymations

Two samples (Table 1N, 1O) were analyzed using GC/MS to confirm thepresence of

the pesticides and PCBs determined previously by GC/ECD.
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Hylebos Reproductive Toxicology in Flatfish Study.

Analyses for Chlorinated Hydrocarbons

Table 1 Notes

The concentrations of analytes were calculated using 4,4'dibromooctafluorobiphenyl as the

surrogate standard.

The “less than” symbol (<) indicates that the analyte was not detected in concentrations

above the stated value.

Results were determined by gas chromatography with electron capture detection (GC/

ECD).

Concentrations less than 10 ng/g are rounded to two significant figures; concentrations

greater than or equal to 10 ng/g are rounded to three significant figures.

The percent recoveries of the surrogate standard were calculated using

tetrachloro-o-xylene to correct for the fraction of the total extract used for the HPLC

cleanup step.

The concentration reported for “Total PCBs” is the sum of the 17 PCB congeners multiplied

by 2 (as defined in the QA Plan).

Set # and Sample # designations are intended for internal lab use and identification only.

Jar # and site name represent Hylebos Damage Assessment official sample identification designations.

The sample weights used to calculate concentrations for the method blank are the mean

sample weights calculated for the field samples in the same set.
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Hylebos Reproductive Toxicology in Flatfish Study.

Analyses for Aromatic Hydrocarbons

Table 2 Notes

The concentrations of the analytes naphthalene and 2-methyinaphthalene were calculated using

naphthalene-d8 as the surrogate standard; analytes from acenaphthylene through pyrene were

calculated using acenaphthene-d10 as the surrogate standard; and analytes from

benz[alanthracene through benzo[ghi]perylene were calculated using benzo[a]pyrene-d12 as the

surrogate standard.

The “less than” symbol (<) indicates that the analyte was not detected in concentrations above the

stated value.

Results were determined by gas chromatography/mass spectrometry (GC/MS).

Concentrations less than 10 ng/g are rounded to two significant figures; concentrations greater than

or equal to 10 ng/g are rounded to three significant figures.

The percent recoveries of the surrogate standards were calculated using phenanthrene-d10 to correct

for the fraction of the total extract used for the HPLC clean-up step.

Set # and Sample # designations are intended for internal lab use and identification only. Jar # and

site name represent Hylebos Damage Assessment official sample identification designations.

The sample weights used to calculate concentrations for the method blank are the mean sample

weights calculated for the field samples in the same set.
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