Printing Date 2013-09-02 12:44:14 UTC 

Restriction of specific regulatory purposes 

 

Confidentiality 

 

Owner (TS 204240) 1-Propanesulfonic acid, 2-hydroxy-3-(2-propen-1-yloxy)-, sodium salt (1:1) / WIL Research Europe B.V. / 'sHertogenbosch / Netherlands 

Legal entity 

owner 

WIL Research Europe B.V. / 's-Hertogenbosch / Netherlands 

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Endpoint study record: Short-term toxicity to fish. WIL Research 

500677 

Administrative Data 

Data source 

Reference 

Materials and methods 

Test guideline 

GLP compliance 

Test materials 

Identity of test material same as for substance defined in section 1 (if not read-across) 

Test material identity 

Test material form 

Details on test material 

UUID 

 

IUC5-9e505c14-2e1d-4ecd-8112-48a34ab4665b 

Dossier UUID 

 

0 

Author 

 

BOL / WIL Research Europe B.V. / 's-Hertogenbosch / Netherlands 

Date 

 

2013-09-02 12:43:29 UTC 

Remarks 

 

 

Purpose flag key study; robust study summary 

Study result type experimental result Study period 13 May 2013 - 17 May 2013 

Reliability 1 (reliable without restriction) 

Rationale for reliability incl. 

deficiencies 

This study has been performed according to OECD and/or EC guidelines and according to GLP 

principles. 

Reference 

type 

Author Year Title Bibliographic 

source 

Testing 

laboratory 

Report 

no. 

Owner 

company 

Company 

study no. 

Report 

date 

study 

report 

L.M. 

Bouwman 

2013 96-HOUR ACUTE 

TOXICITY STUDY IN 

FATHEAD MINNOW 

WITH 1-

PROPANESULFONIC 

ACID, 2-HYDROXY-3-

(2-PROPEN-1-

YLOXY)-, SODIUM 

SALT (1:1) (STATIC) 

- WIL Research 

Europe B.V., 

Hambakenwetering 

7, 5231 DD, 'sHertogenbosch, 

The Netherlands 

500677 Society of 

Chemical 

Manufacturers 

and Affiliates, 

1850 M Street, 

NW Suite 700, 

WASHINGTON, 

DC 20036 USA 

- 2013-

07-18 

Qualifier Guideline Deviations 

according to EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test) (1996) no 

according to OECD Guideline 203 (Fish, Acute Toxicity Test) (1992) no 

yes 

yes 

Identifier Identity 

CAS number 52556-42-0; 123-34-2 and 7732-18-5 

other: Clear colourless liquid 

Molecular formula: C6H11NaO5S 

Molecular weight: 218.22 

Description: Clear colourless liquid 

Batch: 12E1101 

Purity/Composition 

Sodium-1-allyloxy-2- 

hydroxypropane sulphonate 3-allyl-1,2-propanediol 

Water 

CAS Number 

52556-42-0 

123-34-2 

7732-18-5 

Percent 

38.64 

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Analytical monitoring 

Details on sampling 

Details on analytical methods 

Vehicle 

Details on test solutions 

Test organisms 

Test organisms (species) 

Details on test organisms 

Study design 

Test type 

Water media type 

2.26 

59.1 

Test substance storage: At room temperature in the dark

Stability under storage conditions: Stable 

Expiry date: 13 August 2013 

Purity/composition correction factor required: No 

Hygroscopic: No 

Volatile: No 

Specific Gravity / Density: 1.17 

Stability in water: 1 year 

yes 

Samples for possible analysis were taken from the control and both replicates of the test concentration according to the schedule below. 

Frequency at t=0 h, t=48 h and t=96 h 

Volume 1.5 ml 

Storage Samples were stored in a freezer until analysis. 

Additionally, reserve samples of 1.5 ml were taken from all test solutions for possible analysis. If not already used, these samples were stored 

in a freezer for a maximum of three months after delivery of the draft report, pending on the decision of the sponsor for additional analysis. 

The samples were stored in the freezer (≤ -15°C). Storage stability under these conditions was demonstrated in WIL Research project 500674.

On the day of analysis, samples were defrosted at room temperature and analysed. If necessary, the samples were diluted with ISO-medium 

to obtain concentrations within the calibration range. 

Quantitative analysis was based on the analytical method validated for the test substance in WIL Research project 500674 

Analytical method: 

Instrument: Alliance Separation Module 2695 (Waters, Milford, MA, USA) 

Detector: Refractive Index Detector 2414 (Waters) 

Column: 

Stationary phase: Synergi Hydro-RP 80A 

Dimensions: 250 mm x 4.6 mm i.d., dp =4 μm 

Brand: Phenomenex, Torrance, CA, USA 

Column temperature: 40°C +/- 1°C 

Injection volume: 50 μl 

Mobile phase: water containing 0.05M Na2HPO4, 0.05M NaH2PO4 and 0.05M NaNO3 

Flow: 1 ml/min 

RI detection 

Temperature: 30°C 

Sensitivity: 4 

no 

The batch of 1-Propanesulfonic acid, 2-hydroxy-3-(2-propen-1-yloxy)-, sodium salt (1:1) tested was a clear colourless liquid and completely 

soluble in test medium at the concentration tested.

Preparation of test solutions started with a nominal concentration of 1000 mg/l, containing 386 mg of the active ingredient (a.i.) Sodium-1-

allyloxy-2-hydroxypropane sulphonate. Fifteen minutes of magnetic stirring was applied to accelerate the dissolving of the test substance in the 

test medium. The final test concentration was clear and colourless. 

Pimephales promelas 

Species: Fathead minnow (Pimephales promelas, Teleostei, Cyprinidae) Rafinesque 

Source: In house culture (Batch FM 13-02) 

Mean length: 1.9 ± 0.1 cm 

Mean weight: 0.14 ± 0.04 g 

Reason for selection: This system has been selected as an internationally accepted species. 

Feeding: Daily with pelleted fish food (Cyprico Crumble Excellent (300-500 um), Coppens International bv, Helmond, The Netherlands)

Validity of batch: In the batch of fish used for the test, mortality during the seven days prior to the start of the test was less than 5%. 

static 

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Limit test 

Total exposure duration 

Post exposure observation period 

Test conditions 

Hardness 

Test temperature 

pH 

Dissolved oxygen 

Salinity 

Nominal and measured concentrations 

Details on test conditions 

Reference substance (positive control) 

Results and discussions 

Effect concentrations 

Details on results 

Results with reference substance (positive control) 

freshwater 

yes 

96 h 

none 

180 mg/l expressed as CaCO3 

22.5-23.0°C 

7.5-7.9 

6.8-9.2 mg/l 

not measured 

Nominal: 1000 mg/l 

Analysis of the samples taken at the start of the test showed that the actual test concentration was in agreement with nominal (102-103%). 

This concentration remained stable during the test period (99-101% of initial at the end of the test). Given these results, the effect parameters 

were based on the nominal exposure concentration. 

Test duration: 96 hours 

Test type: Static 

Test vessels: 6.5 litres, all-glass, containing 5 litres of test solution 

Test medium: Adjusted ISO medium with a hardness of 180 mg CaCO3 per litre and a pH of 7.7 ± 0.3. 

Number of fish: 7 for the control and 14 for the test concentration 

Number of replicates: 1 for the control; 2 for the test concentration, containing 7 fish each 

Loading: 0.20 g fish/litre, i.e. 7 fish per 5 litres of test medium 

Illumination: 16 hours photoperiod daily 

Aeration: The test media were not aerated during the test. 

Feeding: No feeding from 24 hours prior to the test and during the total test period 

Introduction of fish: Within 40 minutes after preparation of the test media from a holding tank with comparable water quality parameters and pH 

and temperature differences between test and holding tank media of less than 0.5 unit and 0.5°C. 

Euthanasia: At the end of the test the surviving fish were rapidly killed by exposing them to ca. 1.2% ethylene glycol monophenylether in water.

yes pentachlorophenol (PCP) 

Duration Endpoint Effect 

conc. 

Nominal/Measured Conc. based on Basis for 

effect 

Remarks (e.g. 95% CL) 

24 h LC50 > 1000 

mg/L 

nominal test mat. (analytically confirmed nominal 

concentration) 

mortality LC50: >386 mg active 

ingredient /L 

48 h LC50 > 1000 

mg/L 

nominal test mat. (analytically confirmed nominal 

concentration) 

mortality LC50: >386 mg active 

ingredient /L 

72 h LC50 > 1000 

mg/L 

nominal test mat. (analytically confirmed nominal 

concentration) 

mortality LC50: >386 mg active 

ingredient /L 

96 h LC50 > 1000 

mg/L 

nominal test mat. (analytically confirmed nominal 

concentration) 

mortality LC50: >386 mg active 

ingredient /L 

Behavioural abnormalities: none 

The 96h-LC50 was 0.20 mg/l based on nominal concentrations, with a 95% confidence interval between 0.16 and 0.32 mg/l. This effect was 

already reached within 72 hours of exposure. 

No historical data is available on the range of the 96h-LC50 for Fathead minnow. However, this test shows that toxicity is similar for Fathead 

minnow and carp (Teleostei Cyprinidae). For Carp the 96h-LC50 is generally between 0.10 and 0.46 mg/l based on historical data of reference 

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Reported statistics and error estimates 

Applicant's summary and conclusion 

Validity criteria fulfilled 

Conclusions 

tests performed approximately every 3 months from April 1988 until the end of 2000, and annually since then. 

No LC50 could be calculated because the test substance proved to be non-toxic (LC50 > maximum concentration tested). 

yes 

1-Propanesulfonic acid, 2-hydroxy-3-(2-propen-1-yloxy)-, sodium salt (1:1) induced no visible effects in fathead minnow at an analytically 

confirmed nominal concentration of 1000 mg/l. This concentration contained 386 mg of the active ingredient per litre. 

Hence, the 96h-LC50 was >1000 mg test substance/l, corresponding to >386 mg a.i./l. 

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