# STUDY NO. 00-4208

SPONSOR STUDY NO. 211-MTBE-2G

GASOLINE MTBE VAPOR CONDENSATE

A TWO-GENERATION WHOLE-BODY INHALATION REPRODUCTION

TOXICITY STUDY IN RATS

Final Report

Volume III of IV

Submitted to: American Petroleum Institute (API)

1220 L Street, Northwest

Washington, D.C. 20005-4070

Attn: Thomas M. Gray, M.S., D.A.B.T.

Date: 14 June 2011

Page 966 of 1650

# Huntingdon Life Sciences 00-4208 Page 967

211-MTBE-2G Final Report

TABLE OF CONTENTS

Volume III

COVER PAGE .................................................................................................................966

TABLE OF CONTENTS .................................................................................................967

APPENDICES

AA. Individual Animal Gross and Mi croscopic Observations .......................................... 968

P 0 

# Generation

F 1 

# Generation

BB. Individual Animal Gross and Microscopic Observations for Neuropathology

Evaluations of F 1 

# Pups .............................................................................................. 1387

# Huntingdon Life Sciences 00-4208 Page 968

211-MTBE-2G Final Report

Individual Animal Gross

and Microscopic Observations

Preface Appendix AA

Table of Contents

P 0 

# Generat ion ................................................................................................... 969

F 1 

# Generat ion ................................................................................................. 1179

Key to Abbreviations

SC = Spinal Cord

Gang = Ganglia

Fibr = Fiber

Ventrl = Ventral

Gl = Gland

LN = Lymph Node



# Oviducts/Fallop = Oviducts/Fallopian

WNL = Within Normal Limits

Notes

1. “No microscopic observations on tissue” indicates that the pathologist did not

examine the tissue.

2. Unless otherwise specified in a histopathology note, the organ/tissue

examined was the requir ed (routine) section.

3. The dimensions of gross lesions are presented as a general range based on

the actual dimensions noted during macr oscopic examinations. The actual

dimensions of gross lesions can be found in the raw data.

4. The lungs for non-pregnant females were not required to be saved and this is

indicated by the notation “Tissue not saved per memo”.

Corresponding exposure levels for each group were as follows:

Group 1 - 0 mg/m 3



# Group 2 - 2,000 mg/m 3



# Group 3 - 10,000 mg/m 3



# Group 4 - 20,000 mg/m 3Page 969Page 970Page 971Page 972Page 973Page 974Page 975Page 976Page 977Page 978Page 979Page 980Page 981Page 982Page 983Page 984Page 985Page 986Page 987Page 988Page 989Page 990Page 991Page 992Page 993Page 994Page 995Page 996Page 997Page 998Page 999Page 1000Page 1001Page 1002Page 1003Page 1004Page 1005Page 1006Page 1007Page 1008Page 1009Page 1010Page 1011Page 1012Page 1013Page 1014Page 1015Page 1016Page 1017Page 1018Page 1019Page 1020Page 1021Page 1022Page 1023Page 1024Page 1025Page 1026Page 1027Page 1028Page 1029Page 1030Page 1031Page 1032Page 1033Page 1034Page 1035Page 1036Page 1037Page 1038Page 1039Page 1040Page 1041Page 1042Page 1043Page 1044Page 1045Page 1046Page 1047Page 1048Page 1049Page 1050Page 1051Page 1052Page 1053Page 1054Page 1055Page 1056Page 1057Page 1058Page 1059Page 1060Page 1061Page 1062Page 1063Page 1064Page 1065Page 1066Page 1067Page 1068Page 1069Page 1070Page 1071Page 1072Page 1073Page 1074Page 1075Page 1076Page 1077Page 1078Page 1079Page 1080Page 1081Page 1082Page 1083Page 1084Page 1085Page 1086Page 1087Page 1088Page 1089Page 1090Page 1091Page 1092Page 1093Page 1094Page 1095Page 1096Page 1097Page 1098Page 1099Page 1100Page 1101Page 1102Page 1103Page 1104Page 1105Page 1106Page 1107Page 1108Page 1109Page 1110Page 1111Page 1112Page 1113Page 1114Page 1115Page 1116Page 1117Page 1118Page 1119Page 1120Page 1121Page 1122Page 1123Page 1124Page 1125Page 1126Page 1127Page 1128Page 1129Page 1130Page 1131Page 1132Page 1133Page 1134Page 1135Page 1136Page 1137Page 1138Page 1139Page 1140Page 1141Page 1142Page 1143Page 1144Page 1145Page 1146Page 1147Page 1148Page 1149Page 1150Page 1151Page 1152Page 1153Page 1154Page 1155Page 1156Page 1157Page 1158Page 1159Page 1160Page 1161Page 1162Page 1163Page 1164Page 1165Page 1166Page 1167Page 1168Page 1169Page 1170Page 1171Page 1172Page 1173Page 1174Page 1175Page 1176Page 1177Page 1178Page 1179Page 1180Page 1181Page 1182Page 1183Page 1184Page 1185Page 1186Page 1187Page 1188Page 1189Page 1190Page 1191Page 1192Page 1193Page 1194Page 1195Page 1196Page 1197Page 1198Page 1199Page 1200Page 1201Page 1202Page 1203Page 1204Page 1205Page 1206Page 1207Page 1208Page 1209Page 1210Page 1211Page 1212Page 1213Page 1214Page 1215Page 1216Page 1217Page 1218Page 1219Page 1220Page 1221Page 1222Page 1223Page 1224Page 1225Page 1226Page 1227Page 1228Page 1229Page 1230Page 1231Page 1232Page 1233Page 1234Page 1235Page 1236Page 1237Page 1238Page 1239Page 1240Page 1241Page 1242Page 1243Page 1244Page 1245Page 1246Page 1247Page 1248Page 1249Page 1250Page 1251Page 1252Page 1253Page 1254Page 1255Page 1256Page 1257Page 1258Page 1259Page 1260Page 1261Page 1262Page 1263Page 1264Page 1265Page 1266Page 1267Page 1268Page 1269Page 1270Page 1271Page 1272Page 1273Page 1274Page 1275Page 1276Page 1277Page 1278Page 1279Page 1280Page 1281Page 1282Page 1283Page 1284Page 1285Page 1286Page 1287Page 1288Page 1289Page 1290Page 1291Page 1292Page 1293Page 1294Page 1295Page 1296Page 1297Page 1298Page 1299Page 1300Page 1301Page 1302Page 1303Page 1304Page 1305Page 1306Page 1307Page 1308Page 1309Page 1310Page 1311Page 1312Page 1313Page 1314Page 1315Page 1316Page 1317Page 1318Page 1319Page 1320Page 1321Page 1322Page 1323Page 1324Page 1325Page 1326Page 1327Page 1328Page 1329Page 1330Page 1331Page 1332Page 1333Page 1334Page 1335Page 1336Page 1337Page 1338Page 1339Page 1340Page 1341Page 1342Page 1343Page 1344Page 1345Page 1346Page 1347Page 1348Page 1349Page 1350Page 1351Page 1352Page 1353Page 1354Page 1355Page 1356Page 1357Page 1358Page 1359Page 1360Page 1361Page 1362Page 1363Page 1364Page 1365Page 1366Page 1367Page 1368Page 1369Page 1370Page 1371Page 1372Page 1373Page 1374Page 1375Page 1376Page 1377Page 1378Page 1379Page 1380Page 1381Page 1382Page 1383Page 1384Page 1385Page 1386

# Huntingdon Life Sciences 00-4208 Page 1387

211-MTBE-2G Final Report

Individual Animal Gross

and Microscopic Observations for

Neuropathology Evaluations of F 1 

# Pups

Preface Appendix BB

Key to Abbreviations

SC = Spinal Cord

Gang = Ganglia

Fibr = Fiber

Ventrl = Ventral

Note

Unless otherwise specified in a hist opathology note, the organ/tissue examined

was the required (routine) section.

Corresponding exposure levels for each group were as follows:

Group 1 - 0 mg/m 3



# Group 2 - 2,000 mg/m 3



# Group 3 - 10,000 mg/m 3



# Group 4 - 20,000 mg/m 3Page 1388Page 1389Page 1390Page 1391Page 1392Page 1393Page 1394Page 1395Page 1396Page 1397Page 1398Page 1399Page 1400Page 1401Page 1402Page 1403Page 1404Page 1405Page 1406Page 1407Page 1408Page 1409Page 1410Page 1411Page 1412Page 1413Page 1414Page 1415Page 1416Page 1417Page 1418Page 1419Page 1420Page 1421Page 1422Page 1423Page 1424Page 1425Page 1426Page 1427