Effluent Quality Assessment 

Jefferson Smurfit Corporation 

Fernandina Beach Mill 

Nassau County 

NPDES #FL0001104 

Sampled February 16, 2009 

June 2009

Biology Section 

Bureau of Laboratories 

Division of Environmental Assessment and Restoration 

Quality Manual No. 870346G 

NELAC Certification No. E31780
1

Jefferson Smurfit Corporation, Fernandina Beach Mill, North 8th Street, Fernandina Beach, 

Nassau County, Florida, 32035. NPDES #FL0001104. Effluent samples for bioassay and 

chemical analyses for this facility were collected on February 16, 2009. 

Introduction

Jefferson Smurfit Corporation, Fernandina Beach Mill, is an industrial facility which manufactures 

Kraft linerboard using an unbleached Kraft process. The facility has an industrial wastewater 

treatment system consisting of: 

• grit chamber with mechanical screening; 

• primary clarifier; 

• wastewater cooling tower; 

• a UNOX activated sludge system; 

• dual secondary clarifiers; 

• a holding pond for influent loading control; 

• dewatering presses for primary and secondary wastewater solids; 

• blending tank systems for primary and secondary solids. 

Effluent discharge from the facility is continuous and rainfall dependent. The facility has a design 

flow of 37.5 million gallons per day (MGD) (30 MGD design wastewater flow and 7.5 MGD 

stormwater flow) annual average daily flow (AADF). The AADF through the facility from January 

2008 through December 2008 was 16.39 MGD. 

Treated wastewater is discharged through a Parshall flume (outfall D-001) to the Class III Marine 

waters of the Amelia River via a multiport diffuser. The diffuser is 76.2 m (250 feet) long and has 

20 ports spaced at 3.8 m (12.5 feet) intervals. The point of discharge is located approximately at 

latitude 30o 41’ 09” N, longitude 81o 27’ 32” W. Sludge is disposed of at an off-site, company 

owned landfill located in Callahan, Nassau County, Florida. 

The facility has no current active Administrative Orders or Consent Orders. The facility has a 

mixing zone for dissolved oxygen, temperature, chronic toxicity, copper, nickel, and pH for 

effluent discharged from Outfall D-001 into the Amelia River. The mixing zone measures 106.22 

m in length by 20 m in width and has a surface are of 2,124 m2

. The mixing zone includes the 

entire water column from the surface to the bottom, and runs parallel to, and is centered on, the 

diffuser structure. A review of the facility’s Discharge Monitoring Report (DMR) data for the past 

year revealed several violations of permit conditions. The facility is required to prepare and to 

follow a Best Management Practices Plan. The facility completed an in-kind project to install five 

additional conductivity meters (total = 12) in its internal sewer pipelines. The facility submitted the 

final report of its Pollution Prevention Plan on July 16, 2006. 

Date Parameter & Units Limit Result

03/2008 pH (SU) 6.0 - 9.0 5.9

04/2008 Total Recoverable Nickel (mg/L) 24.2 29

05/2008 Total Recoverable Nickel (mg/L) 24.2 41

06/2008 Temperature (°F) 105 105.7

06/2008 Total Recoverable Nickel (mg/L) 24.2 27

List of permit violations (DMR data) and plant violations that 

occurred within the last year:

The results of a third year inspection performed on May 19, 2008, showed that the facility’s 

effluent was acutely toxic to Ceriodaphnia dubia, the AGP was less than the “problem” threshold 

of 10 mg/L dry-weight/L and effluent iron (310 μg/L) and nickel (39.2 μg/L) exceeded Class III 

Marine water quality criteria and/or permit conditions. (Facility information provided by Heather 

Webber of FDEP Northeast District.) 
2

Methods 

Samples were collected from the EFF-1 NPDES sampling point for Outfall D-001 by Eesa Ali and 

Stephen Nye (FDEP Northeast District) following DEP-SOP-001/01 FS 2400 Wastewater 

Sampling. All DEP SOPs are available on the web at: http://www.floridadep.org/labs/qa/sops.htm. 

See Appendix A for the completed chain of custody form. 

The 48-hour screening toxicity tests and algal growth potential test were performed following 

internal DEP SOPs TA07_01, TA07_02, and TA08_01 through TA08_09. All internal DEP SOPs 

are available on the web at: http://www.floridadep.org/labs/cgi-bin/sop/biosop.asp. Prior to test 

initiation, bioassay samples were aerated for 30 minutes at approximately 100 bubbles per 

minute, to decrease the dissolved oxygen concentration from 10.5 mg/L to 8.1 mg/L. 

The apparent salinity of the effluent (1.67 parts per thousand (ppth) based on conductivity and 

temperature measurements) exceeded the limit (1 ppth salinity) for performing algal growth 

potential analyses with the freshwater species, Pseudokirchneriella subcapitata (DEP SOP 

TA08_01). Therefore, the AGP analyses were performed as a marine test. The AGP sample was 

salinity adjusted to meet the minimum salinity requirements of the marine test species. 

Toxicity Test Results 

Tests were performed February 17 - 19, 2009. 

Ceriodaphnia dubia 48-hour acute screening bioassay – LC50 > 100%, 0% mortality* in 100% 

 sample at 48 hours 

Cyprinella leedsi 48-hour acute screening bioassay – LC50 > 100%, 0% mortality* in 100% 

 sample at 48 hours 

The bioassay samples were not acutely toxic to the test organisms. 

See Appendix B for bioassay bench sheets. See Appendix C for results of the last 20 standard 

reference toxicant (SRT) tests and the bench sheets and statistical analyses of the SRT tests that 

correspond to these facility tests. See Appendix D for test organism receipts. 

*NOTE: In a 48-hour acute screening test of 100% effluent, mortality of <20% provides 

confidence that the effluent meets the acute toxicity criterion of Florida’s Surface Water Quality 

Standards (Rule 62-302.500 (1)(a)4, F.A.C.). Mortality between 20-50% indicates low to 

moderate levels of toxicity, and further action may be required. Mortality of >50% indicates that 

the effluent fails to meet the minimum requirement to discharge to waters of the state (Rule 62-

4.244(3)(a), F.A.C.). 

Algal Growth Potential 

The salinity-adjusted effluent AGP was 3.0 U (U - Material was analyzed for but not detected; the 

report value is the minimum detection limit) mg dry wt/L of the saltwater species Dunaliella 

tertiolecta. Raschke and Schultz found that AGP values above 10.0 mg dry weight/L represent a 

“problem” threshold for marine receiving waters, implying nutrient enrichment (personal 

communication, Marshall Faircloth, et. al. FDEP, with Ron Raschke USEPA Region 4 1987). The 

analytical chemistry suggests that the effluent is nitrogen-limited. (Table 1; Maloney et al. 1974). 

See Table 1 for AGP results. 
3

Chemistry Results 

Total residual chlorine and total ammonia were not detected in the bioassay sample in the 

laboratory. The total ammonia concentration in the sample collected and preserved for chemical 

analysis was 0.16 Y (Y – The laboratory analysis was from an unpreserved or improperly 

preserved sample. The data may not be accurate) mg/L. Based on the pH, salinity, and 

temperature of the effluent as collected, the calculated unionized ammonia concentration was 

< 0.02 mg/L. 

Aluminum, copper, iron, nickel and zinc were detected in the effluent at levels that comply with 

Class III marine water quality criteria (WQC) (62-302.530, F.A.C.). Chromium-III was also 

detected in the effluent. Arsenic, cadmium and lead were detected between the laboratory 

method detection limits (MDL) and practical quantitation limits (PQL). Effluent Fecal Coliforms 

(2,000 colony forming units/100 mL (cfu/100 mL)) exceeded the Class III marine WQC of < 800 

cfu/100 mL on any one day. 

See Table 2 for results of analytes detected in the effluent, and corresponding limits. See 

Appendix E for a complete list of chemical analyses performed. See Appendix F for additional 

facility information. 

Conclusion 

The effluent sample collected from the EFF-1 NPDES sampling point for Outfall D-001 for this 

facility on February 16, 2009, was not acutely toxic to either test species during the 48-hour acute 

screening bioassays. The effluent AGP result was less than the "problem” threshold for marine 

receiving waters. Effluent Fecal Coliforms (2,000 colony forming units/100 mL (cfu/100 mL)) 

exceeded the Class III marine WQC of < 800 cfu/100 mL on any one day. Other water quality 

samples did not exceed any applicable water quality standards or violate any permit conditions. 

Literature Cited 

Maloney, T. E., W. E. Miller, and D. T. Specht. 1974. The Marine Algal Assay Procedure: 

Bottle Test. National Environmental Research Center Office of Research and 

Development. U. S. EPA, Corvallis, Oregon. 43 p. 
4

Jefferson Smurfit Corporation, Fernandina Beach Mill

Inorganic Total

Location N:P ratio N:P ratio

Effluent* 3.0 U 20.5 11.3

* - sample salinity adjusted to 7ppt

Table 1. Measured algal growth potential (AGP; mg dry weight/L) of the saltwater species 

Dunaliella tertiolecta and ratios of nitrogen to phosphorus for samples collected from the 

Jefferson Smurfit Corporation, Fernandina Beach Mill facility on February 16, 2009.

AGP

(measured)
5

 Jefferson Smurfit Corporation 

Fernandina Beach Mill NPDES# 

FL0001104

Class III 

Criteria Effluent Limits

None Detected - - None Detected

Aluminum ≤ 1500 - 1,060

Arsenic ≤ 50 - 0.49 I

Cadmium ≤ 8.8 - 0.097 I

Calcium (mg/L) - - 97.9

Chromium-III - - 3.8

Copper ≤ 3.7 ≤ 12.8 1.07

Iron ≤ 300 - 190

Lead ≤ 8.5 - 0.5 I

Magnesium (mg/L) - - 36.5

Nickel ≤ 8.3 ≤ 24.2 5.59

Selenium ≤ 71 - 0.2 U

Silver ≤ 2.3 - 0.025 U

Zinc ≤ 86 - 30

Ortho-phosphate - - 0.008 I

Total Phosphorus - Report 0.3 Y

Total Ammonia - Report 0.16 Y

Un-ionized Ammonia - - ≤ 0.02 c

Nitrate and Nitrite - - 0.004 UJY

Total Kjeldahl Nitrogen - Report 3.4 Y

Organic Nitrogen - - 3.24 c

Total Nitrogen - - 3.402 c

Dissolved Oxygen (mg/L) ≥ 4.0 ≥ 1.7 s 10.5 Q£

pH (SU) 6.5 - 8.5 6.0 - 9.0 s 6.2

Conductivity (μmhos/cm) - - 2,913

Temperature (°C) - ≤ 40.56 35.2

Sample Depth (m) - - 1

Total Residual Chlorine (mg/L) ≤ 0.01 - ≤ 0.03 UQ£

Chlorophyll a (μg/L) - Corrected - - 11 I

Phaeophytin (μg/L) - - 1.6 U

Total Suspended Solids (mg/L) - 180.5 dp 53

CBOD, 5 day (mg/L), N - inhibited - 117.9 dp 9 A

Flow (MGD) - Report 16.39 a

Hardness (mg CaCO3) - - 394.76 c

Fecal Coliforms ≤ 400s # - 2,000 *

Value exceeds the Class III Water Quality Criteria or permit limits

a - Annual average

b - Value is calculated based on hardness

c - Value is calculated

d - daily maximum calculated from Lbs/day based on permitted wastewater flow of 30 MGD 

p - permitted value based on 24-hour composite sample

s - Single sample

I - The reported value is between the laboratory method detection limit and the laboratory practical quantitation limit

J - Estimated value

Q - Sample held beyond normal holding time

U - Material analyzed for but not detected; value reported is the method detection limit

Y - The laboratory analysis was from an unpreserved or improperly preserved sample. The data may not be accurate.

 # - If 10 or fewer samples are taken within a month, the single sample limit is 400 CFU (62-302.530(6), F.A.C.)

£ - Analyses performed in bioassay laboratory

* Analysis performed by Columbia Analytical Services, Inc., Jacksonville

Table 2. Effluent limits, Class III Criteria for predominantly marine waters and chemical 

and microbiological data for samples collected from the Jefferson Smurfit Corporation, 

Fernandina Beach Mill facility on February 16, 2009.

Nutrients (mg/L)

General Physical and Chemical Parameters

Microbiology (Number of colony forming units/100ml)

Effluent

Organic Constituents (μg/L)

Metals (μg/L unless otherwise noted)
6

Appendix A. Chain of Custody Form 
7

Appendix B. Bench Sheets 
8

Appendix B. (continued) 
9

Appendix B. (continued) 
10

Appendix B. (continued) 
11

Appendix B. (continued) 
12

Appendix B. (continued) 
13

Appendix C. SRT Bench Sheets and Statistical Analysis 

Florida Department of Environmental Protection

Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - 48-hour Acute Toxicity

Ceriodaphnia dubia (water flea) - Sodium Chloride (NaCl)

Date LC50 Mean LC50 Std Dev Lower Control Limit Upper Control Limit

07/03/07 1.99 2.14 0.22 1.70 2.58

07/31/07 1.94 2.14 0.22 1.71 2.58

09/04/07 1.95 2.13 0.22 1.70 2.57

10/02/07 2.04 2.11 0.21 1.68 2.53

10/30/07 2.35 2.11 0.21 1.68 2.54

12/04/07 2.13 2.09 0.20 1.67 2.51

01/22/08 1.92 2.08 0.20 1.66 2.50

02/05/08 2.25 2.08 0.20 1.68 2.48

03/04/08 2.15 2.08 0.20 1.68 2.49

04/01/08 2.50 2.11 0.22 1.68 2.55

05/06/08 2.19 2.11 0.22 1.68 2.55

06/03/08 1.91 2.10 0.22 1.66 2.54

07/08/08 2.21 2.11 0.22 1.67 2.55

08/05/08 2.07 2.10 0.22 1.67 2.54

09/03/08 2.22 2.09 0.21 1.68 2.51

10/11/08 2.16 2.11 0.20 1.70 2.51

11/04/08 2.22 2.10 0.20 1.70 2.49

12/02/08 2.26 2.12 0.19 1.75 2.49

01/06/09 2.36 2.16 0.16 1.83 2.48

02/03/09 2.24 2.15 0.16 1.83 2.47

Means are of the last 20 SRT tests.

Lower Control Limit = mean - 2 * Std Dev CV = 7.47

Upper Control Limit = mean + 2 * Std Dev EPA reference 75th quartile CV = 29%

Standard Reference Toxicant Data 

Ceriodaphnia dubia 48 hour LC50

1.50

2.00

2.50

3.00

07/03/0707/31/0709/04/0710/02/0710/30/0712/04/0701/22/0802/05/0803/04/0804/01/0805/06/0806/03/0807/08/0808/05/0809/03/0810/11/0811/04/0812/02/0801/06/0902/03/09

Test Date

g/L NaCl

LC50 Mean LC50 Lower Control Limit Upper Control Limit
14

Appendix C. (continued) 

Florida Department of Environmental Protection

Bureau of Laboratories

Standard Reference Toxicant (SRT) Test Data - 48-hour Acute Toxicity

Cyprinella leedsi (bannerfin shiner) - Sodium Chloride (NaCl)

Date LC50 Mean LC50 Std Dev Lower Control Limit Upper Control Limit

07/10/07 8.52 8.36 0.49 7.38 9.34

08/01/07 7.7 8.31 0.51 7.29 9.34

09/05/07 9.06 8.34 0.54 7.26 9.42

10/02/07 8.55 8.43 0.47 7.45 9.35

10/30/07 8.72 8.43 0.47 7.48 9.37

12/04/07 7.71 8.36 0.47 7.41 9.31

01/08/08 8.7 8.35 0.47 7.41 9.30

02/05/08 8.92 8.42 0.46 7.50 9.33

03/04/08 7.89 8.42 0.46 7.50 9.33

04/01/08 8.24 8.42 0.45 7.51 9.33

05/15/08 8.1 8.39 0.51 7.36 9.41

06/03/08 8.21 8.37 0.51 7.34 9.40

07/08/08 8.04 8.33 0.50 7.32 9.33

08/05/08 7.76 8.33 0.50 7.33 9.33

09/03/08 8.04 8.31 0.50 7.30 9.31

10/11/08 7.45 8.23 0.51 7.22 9.24

11/04/08 8.3 8.20 0.48 7.23 9.17

12/02/08 8.14 8.17 0.47 7.23 9.11

01/06/09 8.33 8.16 0.47 7.23 9.09

02/03/09 8.09 8.16 0.47 7.23 9.09

Means are of the last 20 SRT tests.

Lower Confidence Limit = mean - 2 * Std Dev CV = 5.7

Upper Confidence Limit = mean + 2 * Std Dev EPA reference 75th quartile CV = 19%

Standard Reference Toxicant Data 

Cyprinella leedsi 48-hour LC50

4

6

8

10

07/10/0708/01/0709/05/0710/02/0710/30/0712/04/0701/08/0802/05/0803/04/0804/01/0805/15/0806/03/0807/08/0808/05/0809/03/0810/11/0811/04/0812/02/0801/06/0902/03/09

Test Date

g/L NaCl

LC50 Mean LC50 Lower Control Limit Upper Control Limit
15

Appendix C. (continued) 
16

Appendix C. (continued) 
17

Appendix C. (continued) 
18

Appendix C. (continued) 
19

Appendix C. (continued) 

Ceriodaphnia dubia Acute Standard Reference Toxicant Test 

DATE: 2-3-09 DURATION: 48 hr TOXICANT: NaCl 

SPECIES: Ceriodaphnia dubia

RAW DATA: Concentration Number Mortalities 

 (g/L) Exposed 

 0.00 20 0 

 1.50 20 0 

 2.00 20 0 

 2.50 20 20 

 3.00 20 20 

 4.00 20 20 

SPEARMAN-KARBER TRIM: .00% 

SPEARMAN-KARBER ESTIMATES: LC50: 2.24 

95% CONFIDENCE LIMITS ARE NOT RELIABLE. 

Cyprinella leedsi Acute Standard Reference Toxicant Test 

DATE: 2-3-09 DURATION: 48 hr TOXICANT: NaCl 

SPECIES: Cyprinella leedsi

RAW DATA: Concentration Number Mortalities 

 (g/L) Exposed 

 0.00 20 0 

 6.00 20 0 

 7.00 20 2 

 8.00 20 7 

 9.00 20 18 

 10.00 20 20 

SPEARMAN-KARBER TRIM: .00% 

SPEARMAN-KARBER ESTIMATES: LC50: 8.09 

 95% LOWER CONFIDENCE: 7.81 

 95% UPPER CONFIDENCE: 8.39 
20

Appendix D. Test Organism Receipts 
21

Appendix D. (continued) 
22

Appendix E. Chemical Analyses performed on the effluent from the Jefferson Smurfit CorporatiBeach Mill Plant Outfall D-001, sampled on February 16, 2009.

Date Sampled Field ID Analysis Component Result Remark Units M

2/16/2009 11:30 Effluent EFD-1 AGP/LN Algal Growth Potential 3 U mg DryWt/L 3 2/16/2009 11:30 Effluent EFD-1 Chlorophyll/Grain Size/BOD Biochemical Oxygen Demand-5 Day,N-Inhib 9 A mg/L 02/16/2009 11:30 Effluent EFD-1 Chlorophyll/Grain Size/BOD Phaeophytin-a 1.6 U ug/L 12/16/2009 11:30 Effluent EFD-1 Chlorophyll/Grain Size/BOD Chlorophyll-a, Corrected 11 I ug/L 32/16/2009 11:30 Effluent EFD-1 Overflow Fecal Coliforms-Membrane Filter 2,000 #/100 mL 1 2/16/2009 11:30 Effluent EFD-1 Toxicology Bioassay-Acute-Screen-FW-Fish, LC50 100 L LC50 2/16/2009 11:30 Effluent EFD-1 Toxicology Bioassay-Acute-Screen-FW-C.dubia, LC50 100 L LC50 2/16/2009 11:30 Effluent EFD-1 Metals Cadmium 0.097 I ug/L 02/16/2009 11:30 Effluent EFD-1 Metals Calcium 97.9 mg/L 02/16/2009 11:30 Effluent EFD-1 Metals Arsenic 0.49 I ug/L 02/16/2009 11:30 Effluent EFD-1 Metals Magnesium 36.5 mg/L 02/16/2009 11:30 Effluent EFD-1 Metals Aluminum 1060 ug/L 62/16/2009 11:30 Effluent EFD-1 Metals Zinc 30 ug/L 5 2/16/2009 11:30 Effluent EFD-1 Metals Chromium 3.8 ug/L 02/16/2009 11:30 Effluent EFD-1 Metals Iron 190 ug/L 32/16/2009 11:30 Effluent EFD-1 Metals Copper 1.07 ug/L 02/16/2009 11:30 Effluent EFD-1 Metals Silver 0.025 U ug/L 02/16/2009 11:30 Effluent EFD-1 Metals Nickel 5.59 ug/L 02/16/2009 11:30 Effluent EFD-1 Metals Selenium 0.2 U ug/L 02/16/2009 11:30 Effluent EFD-1 Metals Lead 0.5 I ug/L 02/16/2009 11:30 Effluent EFD-1 Nutrients Kjeldahl Nitrogen 3.4 Y mg N/L 02/16/2009 11:30 Effluent EFD-1 Nutrients Ammonia-N 0.16 Y mg N/L 02/16/2009 11:30 Effluent EFD-1 Nutrients Total-P 0.3 Y mg P/L 02/16/2009 11:30 Effluent EFD-1 Nutrients O-Phosphate-P 0.008 I mg P/L 02/16/2009 11:30 Effluent EFD-1 Nutrients TSS 53 mg/L 12/16/2009 11:30 Effluent EFD-1 Nutrients NO2NO3-N 0.004 UJY mg N/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Diazinon 0.055 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Gamma-BHC 0.011 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Parathion Methyl 0.11 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Dieldrin 0.022 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Endosulfan I 0.022 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Endrin Aldehyde 0.022 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Heptachlor 0.082 U ug/L 0
23

Appendix E. (continued)

Date Sampled Field ID Analysis Component Result Remark Units M

2/16/2009 11:30 Effluent EFD-1 Pesticides Methoxychlor 0.055 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Toxaphene 0.82 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Butylate 0.22 UJ ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Metalaxyl 0.27 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Norflurazon 0.16 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Prometryn 0.16 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Alpha-BHC 0.011 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides DDD-p,p' 0.022 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Malathion 0.16 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Phorate 0.055 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides DDT-p,p' 0.033 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Azinphos Methyl 0.22 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Ethion 0.055 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Fenamiphos 0.22 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Fonofos 0.11 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Mevinphos 0.22 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Naled 0.87 UJ ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Endrin 0.055 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Chlorpyrifos Methyl 0.11 U ug/L 0

2/16/2009 11:30 Effluent EFD-1 Pesticides Ametryn 0.055 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Beta-BHC 0.011 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Parathion Ethyl 0.16 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Heptachlor Epoxide 0.022 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Endosulfan Sulfate 0.022 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Aldrin 0.11 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Simazine 0.055 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Chlorpyrifos Ethyl 0.055 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Atrazine 0.055 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Alachlor 0.65 UJ ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Delta-BHC 0.011 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Endosulfan II 0.022 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides DDE-p,p' 0.022 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Chlordane 0.22 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Metribuzin 0.11 U ug/L 0
24

Appendix E. (continued)

Date Sampled Field ID Analysis Component Result Remark Units M

2/16/2009 11:30 Effluent EFD-1 Pesticides Metolachlor 0.55 UJ ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Ethoprop 0.11 UJ ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Bromacil 0.22 U ug/L 02/16/2009 11:30 Effluent EFD-1 Pesticides Hexazinone 0.11 U ug/L 02/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants 2,4-Dichlorophenol 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants 2,4-Dinitrotoluene 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants 2-Methyl-4,6-dinitrophenol 3.2 U ug/L 32/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Anthracene 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Bis(2-chloroethoxy)methane 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Chrysene 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Hexachlorobutadiene 3.2 U ug/L 32/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Indeno(1,2,3-cd)pyrene 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Nitrobenzene 2.1 U ug/L 22/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Phenanthrene 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants 2,6-Dinitrotoluene 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants 4-Chlorophenyl phenyl ether 2.1 U ug/L 22/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Benzo(b)fluoranthene 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Di-n-butyl phthalate 5.3 U ug/L 52/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Hexachlorobenzene 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants N-Nitrosodiphenylamine/ Diphenylamine 3.2 U ug/L 32/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants 2-Nitrophenol 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants 3,3'-Dichlorobenzidine 42 U ug/L 42/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants 4-Chloro-3-methylphenol 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Benzo(k)fluoranthene 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Butyl benzyl phthalate 5.3 U ug/L 52/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Fluorene 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Hexachlorocyclopentadiene 3.2 UJ ug/L 32/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants 1,2,4-Trichlorobenzene 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants 2-Chloronaphthalene 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants 4-Nitrophenol 16 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Dibenzo(a,h)anthracene 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Dimethyl phthalate 11 UJ ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Fluoranthene 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants 2,4-Dimethylphenol 11 U ug/L 1
25

Appendix E. (continued)

Date Sampled Field ID Analysis Component Result Remark Units M

2/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants 2-Chlorophenol 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants 4-Bromophenyl phenyl ether 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Benzidine 110 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Bis(2-chloroisopropyl)ether 3.2 U ug/L 32/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Diethyl phthalate 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Isophorone 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Naphthalene 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants 2,4-Dinitrophenol 16 U ug/L 1

2/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Acenaphthylene 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Bis(2-ethylhexyl)phthalate 16 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Hexachloroethane 3.2 U ug/L 32/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants N-Nitrosodimethylamine 2.1 U ug/L 22/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Benzo(g,h,i)perylene 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Bis(2-chloroethyl)ether 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Di-n-octyl phthalate 1.1 UJ ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Pyrene 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants 2,4,6-Trichlorophenol 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Acenaphthene 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Benzo(a)anthracene 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Benzo(a)pyrene 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants N-Nitrosodi-n-propylamine 2.1 U ug/L 22/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Pentachlorophenol 3.2 UJ ug/L 32/16/2009 11:30 Effluent EFD-1 Priority Organic Pollutants Phenol 1.1 U ug/L 12/16/2009 11:30 Effluent EFD-1 Field Parameter pH 6.21 2/16/2009 11:30 Effluent EFD-1 Field Parameter Temperature 35.15 C 2/16/2009 11:30 Effluent EFD-1 Field Parameter Sample Depth 1 m 2/16/2009 11:30 Effluent EFD-1 Field Parameter Specific Conductance 2913 umhos/cm 2/16/2009 11:50 Equipment Blank Metals Chromium 0.3 U ug/L 02/16/2009 11:50 Equipment Blank Metals Nickel 0.25 U ug/L 02/16/2009 11:50 Equipment Blank Metals Calcium 0.075 U mg/L 02/16/2009 11:50 Equipment Blank Metals Arsenic 0.25 U ug/L 02/16/2009 11:50 Equipment Blank Metals Aluminum 60 U ug/L 62/16/2009 11:50 Equipment Blank Metals Cadmium 0.03 U ug/L 02/16/2009 11:50 Equipment Blank Metals Zinc 5 U ug/L 5 
26

Appendix E. (continued)

Date Sampled Field ID Analysis Component Result Remark Units M

2/16/2009 11:50 Equipment Blank Metals Copper 0.5 U ug/L 02/16/2009 11:50 Equipment Blank Metals Magnesium 0.04 U mg/L 02/16/2009 11:50 Equipment Blank Metals Selenium 0.2 U ug/L 02/16/2009 11:50 Equipment Blank Metals Lead 0.2 U ug/L 02/16/2009 11:50 Equipment Blank Metals Iron 30 U ug/L 32/16/2009 11:50 Equipment Blank Metals Silver 0.01 U ug/L 02/16/2009 11:50 Equipment Blank Nutrients Total-P 0.02 U mg P/L 02/16/2009 11:50 Equipment Blank Nutrients O-Phosphate-P 0.004 U mg P/L 02/16/2009 11:50 Equipment Blank Nutrients Ammonia-N 0.01 U mg N/L 02/16/2009 11:50 Equipment Blank Nutrients Kjeldahl Nitrogen 0.08 U mg N/L 02/16/2009 11:50 Equipment Blank Nutrients NO2NO3-N 0.004 U mg N/L 02/16/2009 11:50 Equipment Blank Pesticides DDD-p,p' 0.022 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Diazinon 0.056 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Hexazinone 0.11 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Norflurazon 0.17 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Phorate 0.056 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Heptachlor 0.011 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Alachlor 0.67 UJ ug/L 02/16/2009 11:50 Equipment Blank Pesticides Beta-BHC 0.011 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides DDT-p,p' 0.033 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Dieldrin 0.022 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Heptachlor Epoxide 0.022 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Azinphos Methyl 0.22 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Bromacil 0.22 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Mevinphos 0.22 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Naled 0.89 UJ ug/L 02/16/2009 11:50 Equipment Blank Pesticides Simazine 0.056 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Endrin Aldehyde 0.022 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Malathion 0.17 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Delta-BHC 0.011 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Methoxychlor 0.056 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Toxaphene 0.83 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Ethoprop 0.11 UJ ug/L 02/16/2009 11:50 Equipment Blank Pesticides Metalaxyl 0.28 U ug/L 0
27

Appendix E. (continued)

Date Sampled Field ID Analysis Component Result Remark Units M

2/16/2009 11:50 Equipment Blank Pesticides Metolachlor 0.56 UJ ug/L 02/16/2009 11:50 Equipment Blank Pesticides Chlordane 0.22 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Endosulfan II 0.022 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Alpha-BHC 0.011 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Aldrin 0.011 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Parathion Methyl 0.11 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Parathion Ethyl 0.17 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Metribuzin 0.11 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Fenamiphos 0.22 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Ethion 0.056 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Chlorpyrifos Ethyl 0.056 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Ametryn 0.056 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Endosulfan I 0.022 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Fonofos 0.11 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Chlorpyrifos Methyl 0.11 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Atrazine 0.056 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Endrin 0.056 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Endosulfan Sulfate 0.022 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides DDE-p,p' 0.022 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Gamma-BHC 0.011 U ug/L 02/16/2009 11:50 Equipment Blank Pesticides Butylate 0.22 UJ ug/L 02/16/2009 11:50 Equipment Blank Pesticides Prometryn 0.17 U ug/L 02/16/2009 11:50 Equipment Blank Priority Organic Pollutants 2,4-Dichlorophenol 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants 2,4-Dinitrophenol 16 U ug/L 12/16/2009 11:50 Equipment Blank Priority Organic Pollutants 3,3'-Dichlorobenzidine 42 U ug/L 42/16/2009 11:50 Equipment Blank Priority Organic Pollutants 4-Bromophenyl phenyl ether 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Acenaphthene 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Benzo(a)anthracene 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Benzo(a)pyrene 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Benzo(k)fluoranthene 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Fluoranthene 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Hexachlorobenzene 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Hexachloroethane 3.1 U ug/L 32/16/2009 11:50 Equipment Blank Priority Organic Pollutants 2,6-Dinitrotoluene 1 U ug/L 1 
28

Appendix E. (continued)

Date Sampled Field ID Analysis Component Result Remark Units M

2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Bis(2-chloroethoxy)methane 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Butyl benzyl phthalate 5.2 U ug/L 52/16/2009 11:50 Equipment Blank Priority Organic Pollutants Di-n-octyl phthalate 1 UJ ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Hexachlorobutadiene 3.1 U ug/L 32/16/2009 11:50 Equipment Blank Priority Organic Pollutants Hexachlorocyclopentadiene 3.1 UJ ug/L 32/16/2009 11:50 Equipment Blank Priority Organic Pollutants 2,4,6-Trichlorophenol 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants 2,4-Dinitrotoluene 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants 4-Chloro-3-methylphenol 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Dibenzo(a,h)anthracene 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Fluorene 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants N-Nitrosodiphenylamine/ Diphenylamine 3.1 U ug/L 32/16/2009 11:50 Equipment Blank Priority Organic Pollutants 2-Chloronaphthalene 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants 2-Methyl-4,6-dinitrophenol 3.1 U ug/L 32/16/2009 11:50 Equipment Blank Priority Organic Pollutants 4-Chlorophenyl phenyl ether 2.1 U ug/L 22/16/2009 11:50 Equipment Blank Priority Organic Pollutants Acenaphthylene 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Benzidine 100 U ug/L 12/16/2009 11:50 Equipment Blank Priority Organic Pollutants Di-n-butyl phthalate 5.2 U ug/L 52/16/2009 11:50 Equipment Blank Priority Organic Pollutants Diethyl phthalate 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Phenol 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants 1,2,4-Trichlorobenzene 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Benzo(g,h,i)perylene 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants N-Nitrosodimethylamine 2.1 U ug/L 22/16/2009 11:50 Equipment Blank Priority Organic Pollutants Nitrobenzene 2.1 U ug/L 22/16/2009 11:50 Equipment Blank Priority Organic Pollutants Pentachlorophenol 3.1 UJ ug/L 32/16/2009 11:50 Equipment Blank Priority Organic Pollutants 2,4-Dimethylphenol 10 U ug/L 12/16/2009 11:50 Equipment Blank Priority Organic Pollutants 2-Nitrophenol 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Benzo(b)fluoranthene 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Bis(2-chloroethyl)ether 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Chrysene 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Dimethyl phthalate 10 UJ ug/L 12/16/2009 11:50 Equipment Blank Priority Organic Pollutants Indeno(1,2,3-cd)pyrene 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants N-Nitrosodi-n-propylamine 2.1 U ug/L 22/16/2009 11:50 Equipment Blank Priority Organic Pollutants 2-Chlorophenol 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants 4-Nitrophenol 16 U ug/L 1
29

Appendix E. (continued)

Date Sampled Field ID Analysis Component Result Remark Units M

2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Anthracene 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Naphthalene 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Phenanthrene 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Pyrene 1 U ug/L 1 2/16/2009 11:50 Equipment Blank Priority Organic Pollutants Bis(2-chloroisopropyl)ether 3.1 U ug/L 32/16/2009 11:50 Equipment Blank Priority Organic Pollutants Bis(2-ethylhexyl)phthalate 16 U ug/L 12/16/2009 11:50 Equipment Blank Priority Organic Pollutants Isophorone 1 U ug/L 1 
30

APPENDIX F. EPA Last Page 

Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

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Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

1 N 2 5 3 F L 0 0 0 1 1 0 4 11 12 0 9 0 2 1 6 18 B 19 S 20 2

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Transaction Code NPDES NUMBER YR/MO/DA Insp Type Inspector Fac Type

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66

Biological Analyses of the Jefferson Smurfit Corporation, 

Fernandina Beach Mill facility effluent sampled on February 16, 

2009. NPDES #FL0001104.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

The Bioassay of the Jefferson Smurfit Corporation, Fernandina 

Beach Mill facility effluent sampled on February 16, 2009. NPDES 

#FL0001104.

Fill Out This Section For All Surface Water Discharger Inspections(CEI, CSI, CBI, PAI, XSI-RI Optional)

Remarks

The Priority Pollutants Analysis of the Jefferson Smurfit 

Corporation, Fernandina Beach Mill facility effluent sampled on 

February 16, 2009. NPDES #FL0001104.