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Hylebos Waterway Fish Injury Studies

Individual Data and Quality Assurance Results

CASE NARRATIVES

Toxicopathic Conditions in Flatfish

Semivolatile Organics (Tables 1, 2)

Calibrations

The calibration data used to quantitate the analytes met the initial (Tables 1J, 2G) and

continuing (Tables IK, 1L and 2H) calibration criteria detailed in the

“Commencement Bay Quality Assurance Plan, 12/95” (QAP).

Method Blank Analysis

Method blanks were analyzed for CHs (Tables 1E, IF) and AHs (Table 2D). The

criteria in the QAP for method blanks were met (no more than 4 analytes to exceed 3

X the MDLs listed in Tables IM, 2I).

Surrogate Recoveries

Surrogate recoveries for samples analyzed by GC/MS for AHs and by GC/ECD for

PCBs, DDTs and pesticides (Tables 1A, 1D, 2A, and 2C) were within the guidelines

detailed in the QA Plan (50-125% recovery).

SRMAnalyses

An aliquot of NIST tissue SRM 1974a was analyzed with each of the sample sets, and

the results (Tables IE, IF, and 2D) met the criteria in the QA plan (>70% of

concentrations for the certified analytes that were present in NIST SRM 1974a in

concentrations greater than 10 times the MDL were within 35% of either end of the

NIST values. Noncertified values for the other analytes in the SRM and

concentrations from samples of the SRM analyzed previously are also shown in the

tables.

Sample Duplicates

Three samples were analyzed in duplicate for CHs (Tables 1H, 1I), one sample was

analyzed in duplicate for AHs (Table 2F) and the criteria in the QAP were met (QA

plan, Table 6.2).
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Reanalyses

There is no plan to reanalyze any samples.

GC/MS Confirmations

Six samples (Table 1N, 1O) were analyzed using GOMS to confirm the

presence of the pesticides and PCBs determined previously by GC/ECD.
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Hylebos Toxicopathic Conditions in Flatfish Study

Analyses for Chlorinated Hydrocarbons

Table 1 Notes

The concentrations of analytes were calculated using 4,4’-

dibromooctafluorobiphenyl as the surrogate standard.

The “Iess than” symbol (<) indicates that the analyte was not detected in

concentrations above the stated value.

Results were determined by gas chromatography with electron capture detection

(GC/ECD).

Concentrations less than 10 ng/g are rounded to two significant figures;

concentrations greater than or equal to 10 ng/g are rounded to three

significant figures.

The percent recoveries of the surrogate standard were calculated using

tetrachloro-o-xylene to correct for the fraction of the total extract used for the

HPLC cleanup step.

The concentration reported for “Total PCBs” is the sum of the 17 PCB congeners

multiplied by 2 (as defined in the QA Plan).

Set # and Sample # designations are intended for internal lab use and

identification only. Jar # and site name represent Hylebos Damage

Assessment official sample identification designations.

The sample weights used to calculate concentrations for the method blank are

the mean sample weights calculated for the field samples in the same set.
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Hylebos Toxicopathic Conditions in Flatfish Study

Analyses for Aromatic Hydrocarbons

Table 2 Notes

The concentrations of the analytes naphthalene and 2-methylnaphthalene were calculated using

naphthalene-d8 as the surrogate standard; analytes from acenaphthylene through pyrene were

calculated using acenaphthene-d10 as the surrogate standard; and analytes from

benz[a]anthracene through benzo[ghi]perylene were calculated using benzo[alpyrene-d12 as

the surrogate standard.

The “less than” symbol (<) indicates that the analyte was not detected in concentrations above

the stated value.

Results were determined by gas chromatography/mass spectrometry (GC/MS).

Concentrations less than 10 ng/g are rounded to two significant figures; concentrations greater

than or equal to 10 ng/g are rounded to three significant figures.

The percent recoveries of the surrogate standards were calculated using phenanthrene-d10 to

correct for the fraction of the total extract used for the HPLC clean-up step.

Set # and Sample # designations are intended for internal lab use and identification only. Jar #

and site name represent Hylebos Damage Assessment official sample identification

designations.

The sample weights used to calculate concentrations for the method blank are the mean sample

weights calculated for the field samples in the same set.
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