57

SECTION C

CONCENTRATIONS OF METABOLITES OF FLUORESCENT

AROMATIC COMPOUNDS (FACs) IN BILE FROM MARINE MAMMALS

Explanatory notes for Table C-1 and C-2

Samples collected by the Alaska Department of Fish and Game are noted by *

after the sample number; other samples were collected by NOAA.

FACsNPH and FACsPHN represent the compounds that fluoresce at naphthalene and

phenanthrene wavelengths. Units are ng FACs per g bile.

† indicates that the animal was visibly oiled.

In specimen TS-HS-23F, the “F” denotes the sample is a fetus.

Bile was not collected from harbor seals JT-2, JT-3, JT-4, and JT-5, however, tissue

samples were collected and analyzed (Section D).
58
59

Table C-1. Concentrations of metabolites of fluorescent aromatic compounds (FACs, NPH for naphthalene, PHN for

phenanthrene; ng/g) in bile from harbor seals. The prefix for all lab numbers is 60.

Field FACs FACs Protein Lab no. Date

sample no. Site NPH PHN mg/ml for ACs collected

TS-HS-1*† Herring Bay/PWS 200,000 95,000 23.0 1324;1325 Apr-89

TS-HS-10*† Herring Bay/PWS 38,000 17,000 27.3 1380;1414;1458 Jun-89TS-HS-11*† Herring Bay/PWS 30,000 15,000 21.9 1388;1425;1459 Jun-89

TS-HS-20* Herring Bay/PWS 68,000 14,000 44.0 1391;1428;1462 Apr-90TS-HS-24* Herring Bay/PWS 36,000 5,400 32.4 1405;1440;1474 Apr-90TS-HS-22* Herring Bay/PWS 28,000 4,200 23.3 1393;1430;1464 Apr-90TS-HS-21* Herring Bay/PWS 22,000 4,000 17.0 1392;1429;1463 Apr-90TS-HS-9*† Herring Bay/PWS 4,900 1,300 68.0 1379;1413;1457 Jun-89TS-HS-8*† Bay of Isles/PWS 360,000 210,000 10.4 1322;1323;1449 Jun-89TS-HS-7*† Bay of Isles/PWS 53,000 36,000 10.9 1378;1397;1412;1424;1448;1477 Jun-89TS-HS-5*† Bay of Isles/PWS 52,000 32,000 13.2 1376;1410;1446 Jun-89TS-HS-25* Bay of Isles/PWS 67,000 12,000 84.4 1406;1441;1475 Apr-90TS-HS-2*† Bay of Isles/PWS 31,000 8,800 12.6 1373;1407;1443 Jun-89TS-HS-4*† Seal Island/PWS 46,000 25,000 15.4 1375;1409;1445 Jun-89TS-HS-3*† Seal Island/PWS 2,300 2,700 4.0 1374;1408;1444 Jun-89TS-HS-6*† Applegate Rocks/PWS 7,000 3,700 16.9 1377;1396;1411;1423;1447;1476 Jun-89TS-HS-19* Agnes Island/PWS 20,000 6,200 21.4 1390;1427;1461 Nov-89

692-HBSL-001 New Year Island/PWS 12,000 1,500 44.0 1309;1338;1304;1343 Mar-90

692-HBSL-003 Galena Bay/PWS 29,000 2,500 40.6 1313;1347;1308;1345 Apr-90

692-HBSL-002 Galena Bay/PWS 17,000 1,100 26.7 1312;1260;1307;1344 Apr-90

TS-HS-23* NE Eleanor Island/PWS 110,000 44,000 53.2 1394;1431;1465 Apr-90

TS-HS-23F* NE Eleanor Island/PWS 3,300 1,800 55.8 1395;1432;1466 Apr-90TS-HS-17*† Perl Island/Outer Kenai 7,700 2,200 14.1 724;758;793;817 Jul-89

TS-HS-18* Big Fort Island/Gulf of AK 1,400 170 14.8 1389;1426;1460 Oct-89

TS-HS-14*† West Amatuli - Barren Islands/Gulf of AK 14,000 8,000 18.5 721;755;779;790 Jun-89

TS-HS-15* Ushagat Island - Barren Islands/Gulf of AK 11,000 3,000 31.4 722;756;791;815 Jun-89TS-HS-16* Ushagat Island - Barren Islands/Gulf of AK 4,400 800 11.4 723;757;792;816 Jun-89TS-HS-12* Perenosa Bay - Afognak/Gulf of AK 5,600 730 16.8 719;753;777;788 Jun-89TS-HS-13* Between Perenosa and Seal Bay/Gulf of AK 7,200 2,200 23.8 720;754;778;789 Jun-89
60

Table C-2. Concentrations of metabolites of fluorescent aromatic compounds (FACs, NPH for naphthalene, PHN for

phenanthrene; ng/g) in bile from Steller sea lions. The prefix for all lab numbers is 60.

Field FACs FACs Protein Lab no. Date

sample no. Site NPH PHN mg/ml for ACs collected

SL-586* The Needle/PWS 37,000 8,500 30.8 739;744;745;775;805 Jul-89

SL-585* The Needle/PWS 19,000 3,700 9.0 738;743;774;804 Jul-89SL-583* The Needle/PWS 29,000 3,100 47.5 736;741;772;822 Jul-89SL-584* The Needle/PWS 31,000 3,000 34.6 737;742;773;823 Jul-89SL-582* The Needle/PWS 19,000 890 35.9 728;762;771;821 Jul-89SL-587* Chiswell Island/Gulf of AK 4,200 390 7.8 740;776;787;806 Jul-89

692-STSL-001 Flat Island/Gulf of AK 38,000 4,600 13.3 1326;1265;1259;1321;1346 Mar-90

SL-580* Ushagat Island - Barren Islands/Gulf of AK 13,000 2,700 39.2 726;760;795;796;819 Jun-89SL-581* Ushagat Island - Barren Islands/Gulf of AK 45,000 2,100 54.4 727;761;770;820 Jun-89SL-579* Ushagat Island - Barren Islands/Gulf of AK 18,000 1,200 29.9 725;759;794;818 Jun-89
61

SECTION D

CONCENTRATIONS OF AROMATIC COMPOUNDS (ACs) IN EDIBLE

TISSUES OF MARINE MAMMAL SAMPLES

Explanatory notes for Tables D-1 through D-12

Results on sample extracts were determined by gas chromatography/mass

spectrometry (GC/MS) using sequenced selected ion monitoring.

A hyphen (-) indicates that the analyte was not detected above the limit of

detection which ranged from 0.02 to 1 ng/g (ppb) wet weight.

Low levels of naphthalene found were often indistinguishable from those in

method blanks and were not reported. They are designated by “a.”

Some samples were analyzed in replicate as part of the quality assurance (QA)

plan—all of the data are included in the tables. Adjacent data columns with an “R”

after the Lab no. are replicate analyses of a sample.

In specimen TS-HS-23F, the “F” denotes the sample is a fetus.

The Steller sea lion samples were collected by the Alaska Department of Fish and

Game as agents under Marine Mammal Protection Act permit 584 issued to the

Alaska Fisheries Science Center, Seattle, WA (T.R. Loughlin).
62
63

Table D-1. Concentrations, ng/g (ppb) wet weight, of aromatic compounds (ACs) in samples of edible tissue of harbor seals.

General area: PWS PWS PWS PWS PWSSite : Herring Bay Herring Bay Herring Bay Herring Bay Herring BaySpecimen : TS-HS-1 TS-HS-9 TS-HS-10 TS-HS-11 TS-HS-20Date collected : Apr-89 Apr-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Apr-90 Apr-90 Apr-90

ACs Tissue type : blubber liver blubber liver muscle blubber liver muscle blubber liver muscle blubber liver muscle

Visibly oiled : Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes No No NoCollector : ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG

Lab no. : 1325 1324 1413 1379 1457 1414 1380 1458 1425 1388 1459 1428 1391 1462

naphthalene a a 95 a a 16 a a 12 a a 13 a aC1-naphthalenes 31 - 17 - - 2 - - 19 - - 4 - -C2-naphthalenes 110 0.8 9 - - - - - 9 - - - - -C3-naphthalenes 190 - 9 - - 10 - - 17 - - - - -C4-naphthalenes 84 - 1 - - 4 - - - - - - - -

acenaphthylene - - - - - - - - - - - - - -acenaphthene - - - - - - - - - - - - - -fluorene 12 - 3 - - - - - - - - - - -C1-fluorene 41 - 8 - - 9 - - 5 - - - - -C2-fluorene 19 - - - - - - - - - - - - -C3-fluorene 14 - - - - - - - - - - - - -phenanthrene 47 - 14 - - 13 - - 6 - - 2 - -

C1-phenanthrenes 81 - 9 - - 34 - - 7 - - - - -C2-phenanthrenes 18 - 1 - - 3 - - - - - - - -C3-phenanthrenes - - - - - - - - - - - - - -C4-phenanthrenes - - - - - - - - - - - - - -dibenzothiophene 46 1 7 - 0.7 11 - - 3 - - 0.3 - -C1-dibenzothiophenes 75 - - - - 37 - - 20 - - - - -C2-dibenzothiophenes 36 - - - - 9 - - - - - - - -

C3-dibenzothiophenes 0.8 - - - - - - - - - - - - -Sum of LACs 800 2 78 - 0.7 130 - - 86 - - 6 - -fluoranthene - - 5 - - 1 - - 3 - - 1 - -pyrene - - 2 - - - - - 2 - - 1 - -C1-fluoranthenes/pyrenes - - - - - - - - - - - - - -benz[a]anthracene - - - - - - - - 0.8 - - - - -chrysene 0.8 - - - - - - - 2 - - - - -C1-chrysenes/benz[a]anthracenes - - - - - - - - - - - - - -

C2-chrysenes/benz[a]anthracenes - - - - - - - - - - - - - -C3-chrysenes/benz[a]anthracenes - - - - - - - - - - - - - -C4-chrysenes/benz[a]anthracenes - - - - - - - - - - - - - -benzo[b]fluoranthene - - - - - - - - - - - - - -benzo[k]fluoranthene - - - - - - - - - - - - - -benzo[a]pyrene - - - - - - - - - - - - - -indeno[1,2,3-cd]pyrene - - - - - - - - - - - - - -

dibenz[a,h]anthracene - - - - 0.5 - - - - - - - - -benzo[ghi]perylene - - - - - - - - - - - - - -Sum of HACs 0.8 - 7 - 0.5 1 - - 8 - - 2 - -sample weight, grams : 3.14 3.10 3.06 3.01 5.09 3.02 2.97 5.10 3.05 3.12 5.11 3.09 3.02 5.09
64

Table D-2. Concentrations, ng/g (ppb) wet weight, of aromatic compounds (ACs) in samples of edible tissue of harbor seals.

General area: PWS PWS PWS PWS PWSSite : Herring Bay Herring Bay Herring Bay Bay of Isles Bay of IslesSpecimen : TS-HS-21 TS-HS-22 TS-HS-24 TS-HS-2 TS-HS-5Date collected : Apr-90 Apr-90 Apr-90 Apr-90 Apr-90 Apr-90 Apr-90 Apr-90 Apr-90 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89

ACs Tissue type : blubber liver muscle blubber liver muscle blubber liver muscle blubber liver muscle blubber liver muscle

Visibly oiled : No No No No No No No No No Yes Yes Yes Yes Yes YesCollector : ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG

Lab no. : 1429 1392 1463 1430 1393 1464 1440 1405 1474 1407 1373 1443 1410 1376 1446

naphthalene 13 a a 13 a a 21 a a 16 a a 15 a aC1-naphthalenes 4 - - 6 2 - 21 - - 3 - 5 2 - 5C2-naphthalenes - - - - 11 - - - - - - - 0.6 - -C3-naphthalenes - - - - - - - - - 3 - - - - -C4-naphthalenes - - - - - - - - - 0.7 - - 2 - -

acenaphthylene - - - - - - - - - - - - - - -acenaphthene - - - - - - - - - - - - - - -fluorene - - - 1 - - - - - - - - - - -C1-fluorene - - - - - - - - - 3 - - 4 - -C2-fluorene - - - - - - - - - - - - - - -C3-fluorene - - - - - - - - - - - - - - -phenanthrene 2 - - 4 0.9 - 6 - - 9 - 3 9 - -

C1-phenanthrenes - - - - 0.5 - - - - 15 - 0.7 17 - -C2-phenanthrenes - - - - 0.8 - - - - 1 - - - - -C3-phenanthrenes - - - - - - - - - - - - - - -C4-phenanthrenes - - - - - - - - - - - - - - -dibenzothiophene 0.3 - - 2 - - 3 - - 8 - - 8 - -C1-dibenzothiophenes - - - - - - - - - 15 - 0.8 22 - -C2-dibenzothiophenes - - - - - - - - - 3 - - 5 - -

C3-dibenzothiophenes - - - - - - - - - - - - - - -Sum of LACs 6 - - 13 15 - 30 - - 61 - 10 70 - 5fluoranthene 1 - - 2 - - 3 - - 2 - - 1 - -pyrene 0.8 - - 2 - - 3 - - 0.3 - - 0.4 - -C1-fluoranthenes/pyrenes - - - - - - - - - - - - - - -benz[a]anthracene - - - - - - 3 - - - - - - - -chrysene - - - 0.7 - - 4 - - - - - - - -C1-chrysenes/benz[a]anthracenes - - - - - - - - - - - - - - -

C2-chrysenes/benz[a]anthracenes - - - - - - - - - - - - - - -C3-chrysenes/benz[a]anthracenes - - - - - - - - - - - - - - -C4-chrysenes/benz[a]anthracenes - - - - - - - - - - - - - - -benzo[b]fluoranthene - - - 0.7 - - 2 - - - - - - - -benzo[k]fluoranthene - - - 0.7 - - 3 - - - - - - 0.4 -benzo[a]pyrene - - - 0.7 - - 2 - - - - - - - -indeno[1,2,3-cd]pyrene - - - - - - 5 - 0.6 - - - - - -

dibenz[a,h]anthracene - - - - - 0.3 10 - 1 - - - - - -benzo[ghi]perylene - - - - - - 4 - 0.3 - - - - - -Sum of HACs 2 - - 7 - 0.3 39 - 2 2 - - 1 0.4 -sample weight, grams : 2.98 2.97 5.06 3.10 3.06 5.03 3.02 3.03 5.00 3.10 3.08 5.01 3.08 3.04 5.07
65

Table D-3. Concentrations, ng/g (ppb) wet weight, of aromatic compounds (ACs) in samples of edible tissue of harbor seals.

General area: PWS PWS PWS PWSSite : Bay of Isles Bay of Isles Bay of Isles Seal IslandSpecimen : TS-HS-7 TS-HS-8 TS-HS-25 TS-HS-3Date collected : Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Apr-90 Apr-90 Apr-90 Jun-89 Jun-89 Jun-89

ACs Tissue type : blubber blubber liver liver muscle muscle blubber liver muscle blubber liver muscle blubber liver muscle

Visibly oiled : Yes Yes Yes Yes Yes Yes Yes Yes Yes No No No Yes Yes YesCollector : ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG

Lab no. : 1424R 1412R 1378R 1397R 1477R 1448R 1323 1322 1449 1441 1406 1475 1408 1374 1444

naphthalene 15 14 a a a a a a a 32 a a a a aC1-naphthalenes 10 6 - - - 1 - - - 24 - - - - 4C2-naphthalenes 34 24 - - 0.7 - 16 - - - - - - - -C3-naphthalenes 80 57 - - 1 - 32 - - - - - - - -C4-naphthalenes 45 24 - - - - 21 - - - - - - - -

acenaphthylene - - - - -- - -- 5 -- - - -acenaphthene - - - - -- - -- - -- - - -fluorene 6 4 - - - - 5 - - - - - - - -C1-fluorene 24 22 - - - - 12 - - - - - - - -C2-fluorene 15 6 - - - - 0.7 - - - - - - - -C3-fluorene 33 10 - - - - 20 - - - - - - - -phenanthrene 34 37 - - - - 24 - - 25 - - 5 - 3

C1-phenanthrenes 63 66 0.8 - 1 1 19 - - - - - 5 - 1C2-phenanthrenes 18 12 - - - - 1 - - - - - - - -C3-phenanthrenes 4 - - - -- - -- - -- - - -C4-phenanthrenes - - - - -- - -- - -- - - -dibenzothiophene 36 38 0.7 - 0.5 0.6 22 0.4 - - - - 3 - -C1-dibenzothiophenes 67 71 - - 2 2 29 - 1 - - - 8 - -C2-dibenzothiophenes 38 34 - - - - 5 - - - - - - - -

C3-dibenzothiophenes 1 - - - -- - -- - -- - - -Sum of LACs 510 410 2 - 5 5 210 0.4 1 54 - - 21 - 8fluoranthene 2 0.8 - - - - - - - 9 - - 2 - -pyrene 1 0.6 - - - - - - - - - - 0.4 - -C1-fluoranthenes/pyrenes - - - - -- - -- - -- - - -benz[a]anthracene - - - - -- - -- - -- - - -chrysene 0.6 - - - -- - -- 5 -- - - -

C1-chrysenes/benz[a]anthracenes - - - - -- - -- - -- - - -C2-chrysenes/benz[a]anthracenes - - - - -- - -- - -- - - -C3-chrysenes/benz[a]anthracenes - - - - -- - -- - -- - - -C4-chrysenes/benz[a]anthracenes - - - - -- - -- - -- - - -benzo[b]fluoranthene - - - - -- - -- - -- - - -benzo[k]fluoranthene - - - - -- - -- - -- - - -benzo[a]pyrene - - - - -- - -- - -- - - -indeno[1,2,3-cd]pyrene - - - - - - - - - 1 - 0.3 - - -

dibenz[a,h]anthracene - - - - - 0.4 - - - - - 0.5 - - -benzo[ghi]perylene - - - - -- - -- - -- - - -Sum of HACs 4 1 - - - 0.4 - - - 15 - 0.8 2 - -sample weight, grams : 3.01 3.10 3.07 3.01 4.98 5.04 3.12 3.10 5.08 2.95 3.00 5.06 3.03 3.12 5.10
66

Table D-4. Concentrations, ng/g (ppb) wet weight, of aromatic compounds (ACs) in samples of edible tissue of harbor seals.

General area: PWS PWS PWSSite : Seal Island Applegate Rocks Agnes IslandSpecimen : TS-HS-4 TS-HS-6 TS-HS-19Date collected : Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Nov-89 Nov-89 Nov-89

ACs Tissue type : blubber liver muscle blubber blubber liver liver muscle muscle blubber liver muscle

Visibly oiled : Yes Yes Yes Yes Yes Yes Yes Yes Yes No No NoCollector : ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG

Lab no. : 1409 1375 1445 1411R 1423R 1396R 1377R 1476R 1447R 1427 1390 1461

naphthalene 14 a a 14 13 a a a a 12 a aC1-naphthalenes 3 - 5 3 4 - - - 2 6 - -C2-naphthalenes - - 0.9 - - - - -- - - -C3-naphthalenes - - - - - - - -- - - -C4-naphthalenes - - - - - - - -- - - -

acenaphthylene - - - - - - - -- - - -acenaphthene - - - - - - - -- - - -fluorene - - - - - - - -- - - -C1-fluorene 0.7 - - - - - - -- - - -C2-fluorene - - - - - - - -- - - -C3-fluorene - - - - - - - -- - - -phenanthrene 3 - - 1 2 - - - - 3 - -

C1-phenanthrenes - - 2 - - - - -- - - -C2-phenanthrenes - - 4 - - - - -- - - -C3-phenanthrenes - - 1 - - - - -- - - -C4-phenanthrenes - - - - - - - -- - - -dibenzothiophene 3 - - - 0.4 - - - - 0.3 - -C1-dibenzothiophenes 2 - 2 - - - - -- - - -C2-dibenzothiophenes - - 0.6 - - - - -- - - -

C3-dibenzothiophenes - - - - - - - -- - - -Sum of LACs 12 - 16 4 6 - - - 2 9 - -fluoranthene - - - 0.6 0.9 - - - - 2 - -pyrene - - - - 0.4 - - - - 1 - 0.4C1-fluoranthenes/pyrenes - - - - - - - -- - - -benz[a]anthracene - - - - - - - -- - - -chrysene - - - - - - - -- - - -

C1-chrysenes/benz[a]anthracenes - - 0.6 - - - - -- - - -C2-chrysenes/benz[a]anthracenes - - - - - - - -- - - -C3-chrysenes/benz[a]anthracenes - - - - - - - -- - - -C4-chrysenes/benz[a]anthracenes - - - - - - - -- - - -benzo[b]fluoranthene - - - - - - - -- - - -benzo[k]fluoranthene - - - - - - - -- - - -benzo[a]pyrene - - - - - - - -- - - -indeno[1,2,3-cd]pyrene - - - - - - - -- - - -

dibenz[a,h]anthracene - - - - - - - -- - - -benzo[ghi]perylene - - - - - - - -- - - -Sum of HACs - - 0.6 0.6 1 - - - - 3 - 0.4sample weight, grams : 3.15 2.98 4.99 3.01 3.00 3.00 3.13 5.06 5.01 2.97 2.93 5.08
67

Table D-5. Concentrations, ng/g (ppb) wet weight, of aromatic compounds (ACs) in samples of edible tissue of harbor seals.

General area: PWS PWS PWS PWS PWSSite : New Year Island Little Green Island Little Green Island L. Green Island L. Green IslandSpecimen : 692-HBSL-001 JT-2 JT-3 JT-4 JT-5Date collected : Mar-90 Mar-90 Mar-90 Mar-90 Mar-90 Mar-90 Mar-90 Mar-90 Mar-90 Mar-90 Mar-90 Mar-90 Mar-90 Mar-90 Mar-90

ACs Tissue type : blubber kidney liver muscle blubber kidney liver liver blubber kidney liver kidney liver kidney liver

Visibly oiled : No No No No No No No No No No No No No No NoCollector : NOAA NOAA NOAA NOAA NOAA NOAA NOAA NOAA NOAA NOAA NOAA NOAA NOAA NOAA NOAA

Lab no. : 1309 1338 1304 1343 1310 1341 1266R 1305R 1311 1342 1306 1340 1371 1339 1372

naphthalene a a a a a a a a a a a a a a aC1-naphthalenes - - - - - - - - - - - - 3 - 3C2-naphthalenes - - 3 - 2 - 1 3 - 1 3 - 2 0.3 3C3-naphthalenes - - - - 1 - - - - - - - - - -C4-naphthalenes - - - - - - - - - - - - - - -

acenaphthylene - - - - 0.8 - - - - - - - - - -acenaphthene - - - - - - - - - - - - - - -fluorene - - - - 0.7 - - - - - - - - - -C1-fluorene - - - - - - - - - - - - - - -C2-fluorene - - - - - - - - - - - - - - -C3-fluorene - - - - - - - - - - - - - - -phenanthrene - - - - - - - - - - - - - - -

C1-phenanthrenes - - - - - - - - - - - - 0.5 - -C2-phenanthrenes - - - - - - - - - - - - - 0.2 -C3-phenanthrenes - - - - - - - - - - - - - - -C4-phenanthrenes - - - - - - - - - - - - - - -dibenzothiophene - - - - - - - - - - - - - 0.2 -C1-dibenzothiophenes - - - - - - - - - - - - - - -C2-dibenzothiophenes - - - - - - - - - - - - - - -

C3-dibenzothiophenes - - - - - - - 1 - - 2 - - - -Sum of LACs - - 3 - 4 - 1 4 - 1 5 - 6 0.7 6fluoranthene - - - - - - 0.2 - - - - - 0.3 - 0.3pyrene - - - - - - 0.3 - - - - - 0.2 - 0.1C1-fluoranthenes/pyrenes 2 - - - - - - - - - - - - - -benz[a]anthracene - - - - - - - - - - - - - - -chrysene - 0.2 - - - - - - - - - 0.1 - 0.1 -

C1-chrysenes/benz[a]anthracenes - - - - - - - - - - - - - - -C2-chrysenes/benz[a]anthracenes - - - - - - - - - - 1 - - - -C3-chrysenes/benz[a]anthracenes - - - - - - - - - - - - - - -C4-chrysenes/benz[a]anthracenes - - - - - - - - - - - - - - -benzo[b]fluoranthene - - - - - - - - 1 - - - - - -benzo[k]fluoranthene - - - - - - - - 2 - - - 0.4 - -benzo[a]pyrene - - - - - - - - - - - - - - -indeno[1,2,3-cd]pyrene - - - - - - - - - - 0.2 - - - -

dibenz[a,h]anthracene - - - - - - - - - - - - - - -benzo[ghi]perylene - - - - - - - - - - - - - - -Sum of HACs 2 0.2 - - - - 0.5 - 3 - 1 0.1 0.9 0.1 0.4sample weight, grams : 3.00 5.07 3.04 5.04 2.99 5.13 3.06 3.02 2.98 5.00 3.01 5.03 3.02 4.99 3.00
68

Table D-6. Concentrations, ng/g (ppb) wet weight, of aromatic compounds (ACs) in samples of edible tissue of harbor seals.

General area: PWS PWS PWS PWSSite : Galena Bay Galena Bay NE Eleanor Island NE Eleanor IslandSpecimen : 692-HBSL-002 692-HBSL-003 TS-HS-23 TS-HS-23FDate collected : Apr-90 Apr-90 Apr-90 Apr-90 Apr-90 Apr-90 Apr-90 Apr-90 Apr-90 Apr-90 Apr-90 Apr-90 Apr-90 Apr-90

ACs Tissue type : blubber kidney liver muscle blubber kidney liver muscle blubber liver muscle blubber liver muscle

Visibly oiled : No No No No No No No No No No No No No NoCollector : NOAA NOAA NOAA NOAA NOAA NOAA NOAA NOAA ADFG ADFG ADFG ADFG ADFG ADFG

Lab no. : 1312 1260 1307 1344 1313 1347 1308 1345 1431 1394 1465 1432 1395 1466

naphthalene a a a a a a a a 14 a a 10 a aC1-naphthalenes - - - - - - - - 7 - - 5 - -C2-naphthalenes 5 - 3 - 4 0.4 1 - 0.9 - - 0.3 - 6C3-naphthalenes - - - - - - - - - - - - - -C4-naphthalenes - - - - - - - - - - - - - -

acenaphthylene - - - - - - - - - - - - - -acenaphthene - - - - - - - - - - - - - -fluorene - - - - - - - - 0.7 - - - - -C1-fluorene - - - - - - - - - - - - - -C2-fluorene - - - - - - - - - - - - - -C3-fluorene - - - - - - - - - - - - - -phenanthrene - - - - - - - - 4 - - 3 - -

C1-phenanthrenes - - - - - - - - 1 - - 1 - 0.5C2-phenanthrenes - - - - - - - - - - - - - -C3-phenanthrenes - - - - - - - - - - - - - -C4-phenanthrenes - - - - - - - - - - - - - -dibenzothiophene - - - - - - - - 0.7 - - 0.3 - -C1-dibenzothiophenes - - - - - - - - - - - - - -C2-dibenzothiophenes - - - - - - - - - - - - - -

C3-dibenzothiophenes - - 1 - - - - - - - - - - -Sum of LACs 5 - 4 - 4 0.4 1 - 14 - - 10 - 6fluoranthene 4 - - - - - - - 1 - - 2 - -pyrene - - - - - - - - 1 - - 2 - 1C1-fluoranthenes/pyrenes - - - - 0.8 - - - - - - - - -benz[a]anthracene - - - - - - - - - - - - - -chrysene - - - - - - - - - - - - - -

C1-chrysenes/benz[a]anthracenes - - - - - - - - - - - - - -C2-chrysenes/benz[a]anthracenes - - - - - - - - - - - - - -C3-chrysenes/benz[a]anthracenes - - - - - - - - - - - - - -C4-chrysenes/benz[a]anthracenes - - - - - - - - - - - - - -benzo[b]fluoranthene - - - - - - - - - - - - - -benzo[k]fluoranthene - - - - - - - - - - - - - -benzo[a]pyrene - - - - - - - - - - - - - -indeno[1,2,3-cd]pyrene 0.9 - - - - - - - - - - - - -

dibenz[a,h]anthracene - - - - - - - - - - - - - -benzo[ghi]perylene - - - - - - - - - - - - - -Sum of HACs 5 - - - 0.8 - - - 2 - - 4 - 1sample weight, grams : 2.98 5.09 3.02 5.12 2.98 5.01 3.01 5.05 3.03 2.98 5.05 3.09 3.06 5.09
69

Table D-7. Concentrations, ng/g (ppb) wet weight, of aromatic compounds (ACs) in samples of edible tissue of harbor seals.

General area: Outer Kenai Gulf of AK Gulf of AK Gulf of AKSite : Perl Island Big Fort Island W. Amatuli Island - Barren Islands Ushagat Island - Barren IslandsSpecimen : TS-HS-17 TS-HS-18 TS-HS-14 TS-HS-15Date collected : Jul-89 Jul-89 Jul-89 Jul-89 Oct-89 Oct-89 Oct-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89

ACs Tissue type : blubber kidney liver muscle blubber liver muscle blubber kidney liver muscle blubber kidney liver muscle

Visibly oiled : Light Light Light Light No No No Moderate Moderate Moderate Moderate No No No NoCollector : ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG

Lab no. : 817 793 758 724 1426 1389 1460 779 790 755 721 815 791 756 722

naphthalene a a a a 14 a a a a a a aaaaC1-naphthalenes - - - - 5 - - - - - - ----

C2-naphthalenes - - 2 - - - - - - - - ----C3-naphthalenes - - - - - - - - - - - ----C4-naphthalenes - - 0.06 - - - - - - - - ----acenaphthylene - - - - - - - - - - - ----acenaphthene - - - - - - - - - - - ----fluorene - - - - - - - - - - - ----C1-fluorene - - 0.06 - - - - - - - - ----C2-fluorene - - - - - - - - - - - ----

C3-fluorene - - - - - - - - - - - ----phenanthrene 2 - - - 2 - - - - - - ----C1-phenanthrenes - - 0.1 - - - - - - - - ----C2-phenanthrenes - - 0.6 - - - - - - - - 0.9 - - -C3-phenanthrenes - - - - - - - - - - - 0.4 - - -C4-phenanthrenes - - - - - - - - - - - 0.1 - - -dibenzothiophene - - - - - - - - - - - ----

C1-dibenzothiophenes - - - - - - - - - - - ----C2-dibenzothiophenes - - 2 - - - - - - - - ----C3-dibenzothiophenes - - - - - - - - - - - ----Sum of LACs 2 - 5 - 7 - - - - - - 1 - - -fluoranthene - - 0.4 - 1 - - - - - - 0.3 - - -pyrene - - 0.3 - 0.8 - - - - - - 0.1 - - -C1-fluoranthenes/pyrenes - - 0.03 - - - - - - - - ----

benz[a]anthracene - - - - - - - - - - - ----chrysene - - - - - - - - - - - ----C1-chrysenes/benz[a]anthracenes - - - - - - - - - - - ----C2-chrysenes/benz[a]anthracenes - - - - - - - - - - - ----C3-chrysenes/benz[a]anthracenes - - 0.2 - - - - - - - - ----C4-chrysenes/benz[a]anthracenes - - - - - - - - - - - 2 - - -benzo[b]fluoranthene - - - - - - - - - - - ----benzo[k]fluoranthene - - - - - - - - - - - ----

benzo[a]pyrene - - - - - - - - - - - ----indeno[1,2,3-cd]pyrene - - - - - - - - - - - ----dibenz[a,h]anthracene - - - - - - - - - - - ----benzo[ghi]perylene - - - - - - - - - - - ----Sum of HACs - - 0.9 - 2 - - - - - - 2 - - -sample weight, grams : 3.06 5.08 5.04 5.11 3.02 3.03 5.03 5.03 5.05 5.23 5.07 3.02 5.03 - 5.05
70

Table D-8. Concentrations, ng/g (ppb) wet weight, of aromatic compounds (ACs) in samples of edible tissue of harbor seals.

General area: Gulf of AK Gulf of AK Gulf of AKSite : Ushagat Island - Barren Islands Perenosa Bay - Afognak Between Perenosa and Seal BaySpecimen : TS-HS-16 TS-HS-12 TS-HS-13Date collected : Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89

ACs Tissue type : blubber kidney liver muscle blubber kidney liver muscle blubber kidney liver muscle

Visibly oiled : No No No No No No No No No No No NoCollector : ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG

Lab no. : 816 792 757 723 777 788 753 719 778 789 754 720

naphthalene a a a a a a a a aaaaC1-naphthalenes - - - - - - - - - - 2 -C2-naphthalenes - - - - - - 1 - - - 1 -C3-naphthalenes - - 0.2 - - - - - ----C4-naphthalenes - - - - - - - - - - - 0.4

acenaphthylene - - - - - - 0.1 - - - 0.1 -acenaphthene - - - - - - - - ----fluorene - - - - - - - - ----C1-fluorene - - - - - - - - ----C2-fluorene - - - - - - - - ----C3-fluorene - - - - - - - - ----phenanthrene 1 - - - 2 - - - ----

C1-phenanthrenes - - 0.5 - - - - - ----C2-phenanthrenes - - 2 - - - 0.6 - - - 0.2 -C3-phenanthrenes - - 0.4 - - - - - ----C4-phenanthrenes - - - - - - - - ----dibenzothiophene - - - - 2 - - - ----C1-dibenzothiophenes - - 0.1 - - - - - ----C2-dibenzothiophenes - - - - - - 2 - - - 0.8 -

C3-dibenzothiophenes - - - - - - - - - - 0.1 -Sum of LACs 1 - 3 - 4 - 4 - - - 4 0.4fluoranthene - - 0.5 - - - 0.3 - - - 0.2 -pyrene - - 0.5 - - - 0.2 - - - 0.1 -C1-fluoranthenes/pyrenes - - 0.6 - - - - - ----benz[a]anthracene - - - - - - - - ----chrysene - - - - - - - - ----

C1-chrysenes/benz[a]anthracenes - - 0.04 - - - - - ----C2-chrysenes/benz[a]anthracenes - - 0.07 - - - - - ----C3-chrysenes/benz[a]anthracenes - - 0.7 - - - - - - - 0.06 -C4-chrysenes/benz[a]anthracenes - - - - - - - - ----benzo[b]fluoranthene - - - - - - - - ----benzo[k]fluoranthene - - - - - - - - ----benzo[a]pyrene - - - - - - - - ----indeno[1,2,3-cd]pyrene - - - - - - - - ----

dibenz[a,h]anthracene - - - - - - - - ----benzo[ghi]perylene - - - - - - - - ----Sum of HACs - - 2 - - - 0.5 - - - 0.4 -sample weight, grams : 3.03 5.21 5.05 5.20 5.04 5.11 5.00 5.16 - 5.06 5.10 5.18
71

Table D-9. Concentrations, ng/g (ppb) wet weight, of aromatic compounds (ACs) in samples of edible tissue of Steller sea lions.

General area: PWS PWS PWSSite : The Needle The Needle The NeedleSpecimen : SL-582 SL-583 SL-584Date collected : Jul-89 Jul-89 Jul-89 Jul-89 Jul-89 Jul-89 Jul-89 Jul-89 Jul-89 Jul-89 Jul-89 Jul-89

ACs Tissue type : blubber kidney liver muscle blubber kidney liver muscle blubber kidney liver muscle

Visibly oiled : No No No No No No No No No No No NoCollector : ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG

Lab no. : 821 771 762 728 822 772 741 736 823 773 742 737

naphthalene a a a a a a a a aaaaC1-naphthalenes - - - - - - - - ----C2-naphthalenes - - - - - - 1 - ----C3-naphthalenes - - - - - - 2 0.3 - - - 0.6C4-naphthalenes - - 0.09 - - - - - ----

acenaphthylene - - - - - - - - ----acenaphthene - - - - - - - - ----fluorene - - - - - - - - ----C1-fluorene - - - - - - - - ----C2-fluorene - - - - - - - - ----C3-fluorene - - - - - - - - ----phenanthrene - - - - - - - - 1 - - -

C1-phenanthrenes - - 0.2 - - - - - ----C2-phenanthrenes - 0.09 0.4 - - - - - ----C3-phenanthrenes - - 0.5 - - - 0.2 - ----C4-phenanthrenes - - 0.1 - - - - - ----dibenzothiophene - - - - - - - - ----C1-dibenzothiophenes - - - - - - - - ----C2-dibenzothiophenes - - 0.2 - - - - - ----

C3-dibenzothiophenes - - - - - - - - ----Sum of LACs - 0.1 1 - - - 3 0.3 1 - - 0.6fluoranthene 0.5 - 0.4 - - 0.06 0.6 - - 0.2 0.1 -pyrene 0.1 0.06 0.3 - - - 0.9 - - 0.1 0.1 -C1-fluoranthenes/pyrenes - - 0.1 - - - - - ----benz[a]anthracene - - - - - - - - ----chrysene - - - - - - - - ----

C1-chrysenes/benz[a]anthracenes - - - - - - - - ----C2-chrysenes/benz[a]anthracenes - - - - - - - - ----C3-chrysenes/benz[a]anthracenes - - 0.3 - - - - - ----C4-chrysenes/benz[a]anthracenes - - - - - - - - ----benzo[b]fluoranthene - - - - - - - - ----benzo[k]fluoranthene - - - - - - - - ----benzo[a]pyrene - - - - - - - - ----indeno[1,2,3-cd]pyrene - - - - - - - - ----

dibenz[a,h]anthracene - - - - - - - - ----benzo[ghi]perylene - - - - - - - - ----Sum of HACs 0.6 0.1 1 - - 0.1 2 - - 0.3 0.2 -sample weight, grams : 3.05 5.06 5.17 5.16 3.03 5.14 5.04 5.09 3.03 5.05 5.03 5.04
72

Table D-10. Concentrations, ng/g (ppb) wet weight, of aromatic compounds (ACs) in samples of edible tissue of Steller sea lions.

General area: PWS PWS Gulf of AKSite : The Needle The Needle Chiswell IslandSpecimen : SL-585 SL-586 SL-587Date collected : Jul-89 Jul-89 Jul-89 Jul-89 Jul-89 Jul-89 Jul-89 Jul-89 Jul-89 Jul-89 Jul-89 Jul-89 Jul-89

ACs Tissue type : blubber kidney liver muscle blubber kidney liver liver muscle blubber kidney liver muscle

Visibly oiled : No No No No No No No No No No No No NoCollector : ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG

Lab no. : 804 774 743 738 805 775 745R 744R 739 806 776 787 740

naphthalene a a a a a a a aa a a a aC1-naphthalenes - - - - - - - -- - - - -C2-naphthalenes - - - - - - - -- - - - -C3-naphthalenes - - 1 1 - - 1 0.7 0.9 - - - 0.7C4-naphthalenes - - - - - - - -- - - - -

acenaphthylene - - - - - - - -- - - - -acenaphthene - - - - - - - -- - - - -fluorene - - - - - - - -- - - - -C1-fluorene - - - - - - - -- - - - -C2-fluorene - - - - - - - -- - - - -C3-fluorene - - - - - - - -- - - - -phenanthrene - - - - - - - -- - - - -

C1-phenanthrenes - - - - - - - -- - - - -C2-phenanthrenes - - - - - - - -- - - - -C3-phenanthrenes - - - - - - - -- - - - -C4-phenanthrenes - - - - - - - -- - - - -dibenzothiophene - - - - - - - -- - - - -C1-dibenzothiophenes - - - - - - - -- - - - -C2-dibenzothiophenes - - - - - - - -- - - - -

C3-dibenzothiophenes - - - - - - - -- - - - -Sum of LACs - - 1 1 - - 1 0.7 0.9 - - - 0.7fluoranthene 0.2 0.07 0.2 - 0.3 0.06 0.3 0.3 - 0.5 - - -pyrene - 0.06 0.1 - - - 0.3 0.2 - 0.1 - - -C1-fluoranthenes/pyrenes - - - - - - - -- - - - -benz[a]anthracene - - - - - - - -- - - - -chrysene - - - - - - - -- - - - -

C1-chrysenes/benz[a]anthracenes - - - - - - - -- - - - -C2-chrysenes/benz[a]anthracenes - - - - - - - -- - - - -C3-chrysenes/benz[a]anthracenes - - - - - - - -- - - - -C4-chrysenes/benz[a]anthracenes - - - - - - - -- - - - -benzo[b]fluoranthene - - - - - - - -- - - - -benzo[k]fluoranthene - - - - - - - -- - - - -benzo[a]pyrene - - - - - - - -- - - - -indeno[1,2,3-cd]pyrene - - - - - - - -- - - - -

dibenz[a,h]anthracene - - - - - - - -- - - - -benzo[ghi]perylene - - - - - - - -- - - - -Sum of HACs 0.2 0.1 0.3 - 0.3 0.1 0.6 0.5 - 0.6 - - -sample weight, grams : 3.01 5.09 5.07 5.04 3.04 5.02 5.07 5.02 5.05 3.07 5.06 5.07 5.15
73

Table D-11. Concentrations, ng/g (ppb) wet weight, of aromatic compounds (ACs) in samples of edible tissue of Steller sea lions.

General area: Gulf of AK Gulf of AK Gulf of AKSite : Flat Island Ushagat Island - Barren Islands Ushagat Island - Barren IslandsSpecimen : 692-STSL-001 SL-579 SL-580Date collected : Mar-90 Mar-90 Mar-90 Mar-90 Mar-90 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89 Jun-89

ACs Tissue type : blubber blubber kidney liver muscle blubber kidney liver muscle blubber kidney kidney liver muscle

Visibly oiled : No No No No No No No No No No No No No NoCollector : NOAA NOAA NOAA NOAA NOAA ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG ADFG

Lab no. : 1326R 1265R 1259 1321 1346 818 794 759 725 819 795R 796R 760 726

naphthalene a a a aa a a aa a a a a aC1-naphthalenes - - - -- - - -- - - - - -C2-naphthalenes - - - 1 0.5 - - - - -----C3-naphthalenes - - - -- - - -- - - - - -C4-naphthalenes - - - -- - - -- - - - - -

acenaphthylene - - - -- - - -- - - - - -acenaphthene - - - -- - - -- - - - - -fluorene - - - -- - - -- - - - - -C1-fluorene - - - -- - - -- - - - - -C2-fluorene - - - -- - - -- - - - - -C3-fluorene - - - -- - - -- - - - - -phenanthrene - 3 - - - 1 - - - -----

C1-phenanthrenes - - - -- - - 0.08 - -----C2-phenanthrenes 2 - - - - - - 1 - -----C3-phenanthrenes 6 12 - - - - - 0.2 - -----C4-phenanthrenes - - - -- - - -- - - - - -dibenzothiophene - - - -- - - -- - - - - -C1-dibenzothiophenes - - - -- - - -- - - - - -C2-dibenzothiophenes 6 - - - - - - 0.6 - -----

C3-dibenzothiophenes - - - - - - - 0.2 - - - - - -Sum of LACs 14 15 - 1 0.5 1 - 2 - -----fluoranthene - 2 - - - - - 0.3 - 0.5 ----pyrene - 2 - - - - - 0.3 - 0.4 ----C1-fluoranthenes/pyrenes - - - - - - - 0.1 - - - - - -benz[a]anthracene - - - -- - - -- - - - - -chrysene - 0.2 - - - - - - - -----

C1-chrysenes/benz[a]anthracenes - - - -- - - 0.04 - -----C2-chrysenes/benz[a]anthracenes - - - -- - - -- - - - - -C3-chrysenes/benz[a]anthracenes - - - - - - - 0.2 - - - - - -C4-chrysenes/benz[a]anthracenes - - - -- - - -- - - - - -benzo[b]fluoranthene - 0.3 - - - - - - - -----benzo[k]fluoranthene - 0.2 - - - - - - - -----benzo[a]pyrene - 0.2 - - - - - - - -----indeno[1,2,3-cd]pyrene - - - -- - - -- - - - - -

dibenz[a,h]anthracene - - - -- - - -- - - - - -benzo[ghi]perylene - - - -- - - -- - - - - -Sum of HACs - 5 - - - - - 0.9 - 0.9 ----sample weight, grams : 3.08 3.05 5.07 3.04 5.10 3.00 5.02 5.14 5.05 3.03 5.07 5.01 5.14 5.18
74

Table D-12. Concentrations, ng/g (ppb) wet weight, of aromatic compounds (ACs) in samples of edible tissue of Steller sea lions.

General area: Gulf of AKSite : Ushagat Island - Barren IslandsSpecimen : SL-581Date collected : Jun-89 Jun-89 Jun-89 Jun-89

ACs Tissue type : blubber kidney liver muscle

Visibly oiled : No No No NoCollector : ADFG ADFG ADFG ADFG

Lab no. : 820 770 761 727

naphthalene aaaaC1-naphthalenes ----C2-naphthalenes - - 2 0.08C3-naphthalenes - - 0.1 -C4-naphthalenes ----

acenaphthylene - - 0.06 -acenaphthene ----fluorene ----C1-fluorene ----C2-fluorene ----C3-fluorene ----phenanthrene ----

C1-phenanthrenes - - 0.3 -C2-phenanthrenes - 0.1 0.7 -C3-phenanthrenes - - 0.5 -C4-phenanthrenes ----dibenzothiophene - - 0.1 -C1-dibenzothiophenes ----C2-dibenzothiophenes - - 1 -

C3-dibenzothiophenes - - 0.7 -Sum of LACs - 0.1 5 0.1fluoranthene 0.3 - 0.4 -pyrene - - 0.5 -C1-fluoranthenes/pyrenes - - 0.3 -benz[a]anthracene ----chrysene ----

C1-chrysenes/benz[a]anthracenes ----C2-chrysenes/benz[a]anthracenes - - 0.04 -C3-chrysenes/benz[a]anthracenes - - 0.3 -C4-chrysenes/benz[a]anthracenes ----benzo[b]fluoranthene ----benzo[k]fluoranthene ----benzo[a]pyrene ----indeno[1,2,3-cd]pyrene ----

dibenz[a,h]anthracene ----benzo[ghi]perylene ----Sum of HACs 0.3 - 2 -sample weight, grams : 3.01 5.13 5.21 5.09