Publications

We thank John Stein and Gina Ylitalo for careful review of this manuscript, and Sharon Giese and Barbara Bennett for editorial comments and assistance. We would like to thank Dr. R. C. Gupta, Dr. M. V. Reddy, Dr. B. Dunn and, in particular, Dr. Kurt Randerath for many helpful discussions on the 32P-postlabeling process. In particular, we are indebted to Dr. Usha Varanasi for her insights and enthusiasm for the studies of genotoxicity in marine species. This work was supported, in part, by funds from NOAA's Coastal Ocean Program and the National Status and Trends Program.

Ballance, P., J. Morgan, G. McGregor, and B. Durkacz. 1984. Health and safety solutions: the preparation of radioactively labeled DNA. Safe Practitioner 2:25-26.

Beach, A. C., and R. C. Gupta. 1992. Human biomonitoring and the 32P-postlabeling assay. Carcinogenesis 13:1053-1074.

Dunn, B. P., J. J. Black, and A. Maccubbin. 1987. 32P-postlabeling analysis of aromatic DNA adducts in fish from polluted areas. Cancer Res. 47:6543-6548.

Gorelick, N. J., and G. N. Wogan. 1989. Fluoranthene-DNA adducts: identification and quantification by HPLC-32P-postlabeling. Carcinogenesis 10:1567-1577.

Gupta, R. C. 1985. Enhanced sensitivity of 32P-postlabeling analysis of aromatic carcinogen DNA adducts. Cancer Res. 45:5656-5662.

Gupta, R. C. 1989. Newly detected DNA damage at physiological temperature. Proc. Am. Assoc. Cancer Res. 30:599.

Gupta, R. C., and K. Earley. 1988. 32P-adduct assay: comparative recoveries of structurally diverse DNA adducts in the various enhancement procedures. Carcinogenesis 9:1687-1693.

Gupta, R. C., and K. Randerath. 1988. Analysis of DNA adducts by 32P-labeling and thin layer chromatography. In E. C. Friedberg and P. H. Hanawalt (editors), DNA Repair, Marcel Dekker, New York, p. 399-418.

Gupta, R. C., M. V. Reddy, and K. Randerath. 1982. 32P-Postlabeling analysis of nonradioactive aromatic carcinogen-DNA adducts. Carcinogenesis 3:1081-1092.

Johnson, L. L., C. M. Stehr, O. P. Olsen, M. S. Myers, S. M. Pierce, C. A. Wigren, B. B. McCain, and U. Varanasi. In press. Chemical contaminants and hepatic lesions in winter flounder (Pleuronectes americanus) from the northeast coast of the United States. Environ. Sci. Technol.

Johnson, R. A., and T. F. Walseth. 1979. The enzymatic preparation of [a-32P]ATP, [32P]GTP, [32P]cAMP,and [32P]GMP, and their use in the assay of adenylate and guanylate cyclases, and cyclic nucleotide phosphodiesterases. Ad. Cyclic Nucleotide Res. 10:135-167.

Josefsen, K., M. Wollike, and K. Buschard. 1993. An alternative approach to 32P radiation protection: source shielding. Nucleic Acids Res. 21:485-487.

Liu, T.-Y., S.-L. Cheng, T.-H. Ueng, Y.-F. Ueng, and C.-W. Chi. 1991. Comparative analysis of aromatic DNA adducts in fish from polluted and unpolluted areas by the 32P-postlabeling analysis. Bull. Environ. Contamin. Toxicol. 47:783-789.

Myers, M. S., L. L. Johnson, T. Hom, T. K. Collier, J. E. Stein, and U. Varanasi. 1993. In prep. Toxicopathic hepatic lesions in subadult English sole from Puget Sound, WA; Relationship to other indicators of contaminant exposure. Available Northwest Fisheries Science Center, 2725 Montlake Blvd. E., Seattle, WA. 98112.

Poginsky, B., B. Blomeke, A. Hewer, D. H. Phillips, L. Karbe, and H. Marquardt. 1990. 32P-postlabelling analysis of hepatic DNA of benthic fish from European waters. Proceed. Am. Assoc. Cancer Res. 31:96.

Randerath, K., and E. Randerath. 1964. Ion-exchange chromatography of nucleotides on polyethyleneimine-cellulose thin layers. J. Chromatogr. 16:111-125.

Randerath, K., E. Randerath, H. P. Agrawal, and M. V. Reddy. 1984. Biochemical (postlabelling) methods for analysis of carcinogen-DNA adducts. In A. Berlin, M. Draper, K. Hemminki, and H. Vanio (editors), Monitoring human exposures to carcinogenic and mutagenic agents, International Agency for Research on Cancer, Lyon, France, p. 217-231.

Ray, S., B. P. Dunn, J. F. Payne, L. Fancey, and P. Belands. 1991. Aromatic DNA-carcinogen adducts in Beluga whales from the Canadian Arctic and Gulf of St. Lawrence. Mar. Pollut. Bull. 22:392-396.

Reddy, M. V., and G. R. Blackburn 1990. 32P-postlabeling assay for carcinogen-DNA adducts: description of beta shielding apparatus and semi-automatic spotting and washing devices that facilitate the handling of multiple samples. Carcinogenesis 11:683-687.

Reddy, M. V., K. Hemminki, and K. Randerath. 1991. Postlabeling analysis of polycyclic aromatic hydrocarbon-DNA adducts in white blood cells of foundry workers. J. Toxico. Environ. Health 34:177-185.

Reddy, M. V., and K. Randerath. 1986. Nuclease P1-mediated enhancement of sensitivity of 32P-postlabeling test for structurally diverse DNA adducts. Carcinogenesis 7:1543-1551.

Reddy, M. V., and K. Randerath. 1987. 32P-analysis of DNA adducts in somatic and reproductive tissues of rats treated with the anticancer antibiotic, mitomycin C. Mutat. Res. 179:75-88.

Reichert, W. L., J. E. Stein, B. French, P. Goodwin, and U. Varanasi. 1992. Storage phosphor imaging technique for detection and quantitation of DNA adducts measured by the 32P-postlabeling assay. Carcinogenesis 13:1475-1479.

Schurdak, M. E., D. B. Stong, D. Warshawsky, and K. Randerath. 1987. 32P-Postlabeling analysis of DNA adduction in mice by synthetic metabolites of the environmental carcinogen, 7H-dibenzo[c,g]carbazole: chromatographic evidence for 3-hydroxy-7H-dibenzo[c,g]carbazole being a proximate genotoxicant in liver but not skin. Carcinogenesis 8:591-597.

Segerback, D., and P. Vodica. 1993. Recoveries of DNA adducts of polycyclic aromatic hydrocarbons in the 32P-postlabeling assay. Carcinogenesis 14:2463-2469.

Sikka, H. C., A. R. Steward, C. Kandaswami, J. P. Rutkowski, J. Zaleski, K. Earley, and R. C. Gupta. 1991. Metabolism of benzo[a]pyrene and persistence of DNA adducts in the brown bullhead (Ictalurus nebulosus). Comp. Biochem. and Physiol.: 100C:25-28.

Slobodien, M. 1980. Radiation hazards in the laboratory. In A. A. Fuscaldo, B. J. Erlick, and B. Hindmann (editors), Laboratory safety: Theory and practice, Academic Press, New York, p. 59-112.

Spencer, G. G., A. C. Beach, and R. C. Gupta. 1993. Improved thin-layer chromatographic separation of 32P-postlabeled DNA adducts. J. Chromatogr. 612:295-301.

Stein, J. E., T. K. Collier, W. L. Reichert, E. Casillas, T. Hom, and U. Varanasi. 1992. Bioindicators of contaminant exposure and sublethal effects: studies with benthic fish in Puget Sound, Washington. Environ. Toxicol. Chem. 11:701-714.

Stein, J. E., W. L. Reichert, B. French, and U. Varanasi. 1993. 32P-postlabeling analysis of DNA adduct formation and persistence in English sole (Pleuronectes vetulus) exposed to benzo[a]pyrene and 7H-dibenzo[c,g]carbazole. Chem-Biol. Interact. 88:55-69.

Swanstrom, R., and P. B. Shank. 1978. X-ray intensifying screens greatly enhance the detection by autoradiography of the radioactive isotopes 32P and 125I. Analyt. Biochem. 86:184-192.

Talaska, G., J. H. Roh, and T. Getek. 1992. Review: 32P-postlabeling and mass spectrometric methods for analysis of bulky, polyaromatic carcinogen-DNA adducts in humans. J. Chromatogr. 580:293-323.

Varanasi, U., W. L. Reichert, and J. E. Stein. 1989a. 32P-postlabeling analysis of DNA adducts in liver of wild English sole (Parophrys vetulus) and winter flounder (Pseudopleuronectes americanus). Cancer Res. 49:1171-1177.

Varanasi, U., W. L. Reichert, B.-T. L. Eberhart, and J. E. Stein. 1989b. Formation and persistence of benzo(a)pyrene-diolepoxide-DNA adducts in liver of English sole (Parophrys vetulus). Chem-Biol. Interact. 69:203-216.

Watson, W. P. 1987. Review: post-labeling for detecting DNA damage. Mutagenesis 2:319-331.

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