186

Hylebos Waterway Fish Injury Studies

Individual Data and Quality Assurance Results

CASE NARRATIVES

Juvenile Salmon Injury

Semivolatile Organics (Tables 1, 2)

Calibrations

The calibration data used to quantitate the analytes met the initial (Tables U, 2G)

and continuing (Tables lK, 1L and 2H) calibration criteria detailed in the

“Commencement Bay Quality Assurance Plan, 12/95” (QAP).

Method Blank Analysis

Method blanks were analyzed for CHs (Tables 1E, IF) and AHs (Table 21)). The

criteria in the QAP for method blanks were met (no more than 4 analytes to exceed

3 X the MDLs listed in Tables 1M, 21).

Surrogate Recoveries

Surrogate recoveries for samples analyzed by GC/MS for AlIs and by GC/ECD for

PCBs, DDTs and pesticides (Tables 1A, 11), 2A, and 2C) were within the

guidelines detailed in the QA Plan (50-125% recovery).

SRMAnalyses

An aliquot of NIST tissue SRM 1974a was analyzed with each of the sample sets,

and the results (Tables 1E, IF, and 2D) met the criteria in the QA plan (>70% of

concentrations for the certified analytes that were present in NIST SRM 1974a in

concentrations greater than 10 times the MIDL were within 35% of either end of

the NIST values. Noncertified values for the other analytes in the SRM are also

shown in the tables.

Sample Duplicates

Four samples were analyzed in duplicate (Tables 1H, 11, and 217) and the criteria

in the QAP were met (QA plan, Table 6.2). In several instances, the concentrations

of analytes were so low that they were not detected in one sample but were

detected in the duplicate--in these cases the RSD may be >50%, but is irrelevant.
187

Matrix Spike Analyses I

The recoveries for the spiked analytes (AHs, PCBs, DDTs, and pesticides;

Tables 1M, 21) were within the 50-125% range for at least 80% of the spiked

analytes as set forth in the QAP.

Reanalyses

There is no plan to reanalyze any samples.

GUMS Confirmations

Four samples (Table 1 N, 10) were analyzed using GOMS to confirm the

presence of the pesticides and PCBs determined previously by GOECD.
188

Hylebos Juvenile Salmon Injury Study

Analyses for Chlorinated Hydrocarbons

Table 1 Notes

The concentrations of analytes were calculated using 4,4'-dibromooctafluorobiphenyl as the surrogate

standard.

The “less than” symbol (<) indicates that the analyte was not detected in concentrations above the stated

value.

Results were determined by gas chromatography with electron capture detection (GC/ECD).

Concentrations less than 10 ng/g are rounded to two significant figures; concentrations greater than or

equal to 10 ng/g are rounded to three significant figures.

The percent recoveries of the surrogate standard were calculated using tetrachloro-o-xylene to correct for

the fraction of the total extract used for the HPLC cleanup step.

The concentration reported for “Total PCBs” is the sum of the 17 PCB congeners multiplied by 2 (as

defined in the QA Plan).

Set # and Sample # designations are intended for internal lab use and identification only. Jar # and site

name represent Hylebos Damage Assessment official sample identification designations.

The sample weights used to calculate concentrations for the method blank are the mean sample weights

calculated for the field samples in the same set.
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218