# Patent

## Title
Modification of lignin synthesis in plants

## Abstract

DNA sequences and plasmids are described, that by integration in a plant genome of sugar beet change the sucrose concentration. In addition, transgenic plants that, by introduction of the DNA sequences of the invention, have altered sugar concentrations, are described.

## Background
The present invention relates to DNA sequences and plasmids, containing these DNA sequences, which by integration into the genome of a sugar-beet plant, changes the sugar metabolism of the plant to be changed. The invention also relates to transgenic plants formed with the help of these sequences.

Sucrose is of central importance for the plant and serves many functions. For the long distance transport of photoassimilates and/or energy between various organs in plants, sucrose is almost exclusively used. The sucrose, which is transported in a specific heterotrophic organ, determines the growth and the development of this organ. Thus it is known, e.g. from EP 442 592, that transgenic plants, in which the transport away of the sucrose from the exporting leaves is inhibited by expression of an apoplastic invertase, shows a strong reduction in the growth of e.g. roots or tubers in the case of potato plants. For tobacco plants, the principal importance of sucrose as the central function for the long distance transport of energy carriers within the plant is described (von Schaewen et al, 1990, EMBO J 9: 3033-3044).

Further it is also known from EP 455 316 that DNA sequences present on plasmids, after introduction in a plant genome of a potato plant can affect the starch biosynthesis as well altering the amount and composition of the protein in the potato tubers.

While it has known that a reduction of the amount of sucrose imported in the heterotrophic organs, such as tubers and seeds, leads to loss of yield, it is not known whether an increase in the amount of sucrose in the photosynthetically active parts of the plant, mainly the leaves, leads to a better supply of heterotrophic organs and thus to an increase in yield.

Besides sucrose and/or the hexoses, glucose and fructose, derived from sucrose, have the property of protection of plants against frost damage at low temperatures. Frost damage is one of the main limiting factors in agricultural productivity in the northern hemisphere. Temperatures below freezing lead to the formation of ice crystals. Since the growing ice crystals consist of pure water, water is extracted from the cells as the temperature falls.

This dehydration has at least two potential damaging results: cell contracts following the loss of water. Highly concentrated salts and organic acids lead to membrane damage. cell membrane also expands again. The volume expansion puts a heavy mechanical load on the membrane.

It is thus clear that a freezing/dew cycle can lead to severe membrane damage of the cells and thus to damage to the plant.

It thus appears worth trying to hinder the freezing. One possible strategy is the increased formation of osmotically active substances in the cytosol of plant cells. This should lead to a lowering of the freezing point. Osmotically active substances include sucrose and/or the two hexoses derived from sucrose.

The increased formation of sucrose and/or the two hexoses at low temperatures is desirable in the growing plant. Another situation can exist in the harvested parts of a plant, especially in storage.

In relation to the economic aspects, sucrose thus possesses two especially important functions: lowering the freezing point in intact, growing plants.

The biosynthesis pathways for the formation of sucrose, either from the primary photosynthesis products (in the leaf) or by breakdown of starch (in the storage organs e.g. of potatoes), are known.

It is however, not known how and in what way changes of the carbohydrate concentration in sugar beet can be achieved since it is not possible to use even very similar genes, such as for example genes that code for a sucrose synthase, ADP-glucose pyrophosphorylase or sucrose phosphate synthase of the potato with satisfactory success for the preparation of sugar beet with changed sucrose concentration. An exact analysis and determination of the DNA sequences or sequence fragments for the sugar beet is thus required.

To change the sugar concentration in sugar beet, DNA sequences are now provided which code for the small and large subunit of the ADP glucose pyrophosphorylase, the sucrose synthase and the sucrose phosphate synthase of sugar beet (Seq. ID No 1-8).

The DNA sequences (Seq. ID No. 1-4) can be introduced into plasmids and in this way combined with steering elements for expression in eukaryotic cells. Such steering elements are, on the one hand, transcription promoters and, on the other hand, transcription terminators. Each plasmid comprises: suitable time point or in the specified developments in the transgenic plant or in determined genes of transgenic plants; which is into a protein, whereby the protein demonstrates an enzymatic activity which leads to a change of the sucrose concentration in the plant, or leads to the formation of a so-called anti-sense RNA which under-expresses the formation of the protein coded from an endogenous gene in the plant, that is involved in the sucrose biosynthesis; and termination and polyadenylation of the transcript.

The coding sequences named under b) are the sequences that code for the large and small subunit of the ADP glucose pyrophosphorylase, for the sucrose phosphate synthase and for the sucrose synthase of sugar beet.

The large subunit of the ADP-glucose-pyrophosphorylase has following nucleotide sequence (Seq. ID Nos. 1 and 2): ##STR1##

The small subunit of the ADP-glucose-pyrophosphalase has the following nucleotide sequence (Seq. ID Nos. 3 and 4): ##STR2##

The sucrose phosphate--synthase has the following nucleotide sequence (Seq. ID Nos. 5 and 6): ##STR3##

The sucrose-synthase has the following nucleotide sequence (Seq. ID Nos. 7 and 8): ##STR4##

These sequences can also be combined together in a suitable plasmid which leads to a combination of the individual characteristics, conditioned by the expression of the protein.

The promoter should ensure that the foreign gene is expressed in the plant. The promoter can be so chosen that the expression occurs only in specified tissues, at a determined time point in the plant's development or at a time point determined by outside influences. The promoter can be homologous or heterologous to the plant. Suitable promoters are e.g. the promoter of the 35S RNA of the cauliflower mosaic virus, the patatin promoter B33 (Rocha-Sosa et al. (1989) EMBO J 8: 23-29) or a promoter that ensures an expression only in photosynthetically active tissues. Other promoters can be used which ensure expression only in specified organs, such as the root, tuber, seed, stem or specified cell types such as mesophyllic, epidermal or transport cells.

The coding sequences described herein contain the information for the formation of an mRNA for the large subunit of the ADP-glucose-pyrophosphorylase and the sucrose-phosphate-synthase (SPS) and a part of the information for formation of the small subunit of the ADP-glucose-pyrophosphorylase as well as the sucrose-synthase, that are suitable for the formation of anti-sense RNA to the corresponding genes. Whether a translatable mRNA or an anti-sense nucleic acid is formed, depends on the orientation of the coding sequence in relation to the promoter. If the 3' end of the coding sequence is fused to the 3' end of the promoter, an anti-sense RNA results, while fusion of the 5' end of the coding to the 3' end of the promoter produces a translatable RNA. The latter leads to an increase of the enzyme activity in the cell, while the former leads to a reduction of the enzyme activity in the cell.

The coding sequence for the large and small subunit of the ADP-glucose-pyrophosphorylase, the sucrose phosphate synthase and the sucrose synthase can be one of those described in this invention or can be one that is derived by modifications of the sequences described above. Thereby especially modifications of the sequences can be considered which lead to by-passing of the plant's own regulation mechanisms. Modifications to the DNA sequences of the invention can be by known methods, such as e.g. base exchange or targeted or non-targeted mutagenesis. The so-formed derivatives of the DNA sequences of the invention are also within the scope of the invention.

With plasmids, which contain one or more of the DNA sequences of the invention, sugar beet can be transformed with the object of raising and/or reducing the enzyme activity and/or the change of the sucrose concentration.

For the introduction of the DNA sequences of the invention in sugar beet, a large number of cloning vectors are available, which contain a replication signal for E. coli and a marker, which allows a selection of the transformed cells.

According to the introduction method of the desired gene in the plant, other DNA sequences may be suitable. Should the Ti- or Ri-plasnid be used, e.g. for the transformation of the plant cell, then at least the right boundary, often however, both the right and left boundary of the Ti- and Ri-Plasmid T-DNA, is attached, as a flanking region, to the gene being introduced. The use of T-DNA for the transformation of plants cells has been intensively researched and is well described in EP 120 516; Hoekama, In: The Binary Plant Vector System, Offset-drukkerij Kanters B. V. Alblasserdam, (1985), Chapter V; Fraley, et al., Crit. Rev. Plant Sci., 4:1-46 and An et al. (1985) EMBO J. 4: 277-287. Once the introduced DNA is integrated in the genome, it is generally stable there and remains also in the offspring of the original transformed cells. It normally contains a selection marker, which induces resistance in the transformed plant cells against a biocide or antibiotic such as kanamycin, G 418, bleomycin, hygromycin or phosphinotricin etc. The individual marker employed should therefore allow the selection of transformed cells from cells, which lack the introduced DNA.

For the introduction of DNA into a plant, besides transformation using Agrobacteria, there are many other techniques available. These techniques include the fusion of protoplasts, microinjection of DNA and electroporation, as well as ballistic methods and virus infection. From the transformed plant material, whole plants can be regenerated in a suitable medium, which contains antibiotics or biocides for the selection. The resulting plants can then be tested for the presence of introduced DNA. No special demands are placed on the plasmids in injection and electroporation. Simple plasmids, such as e.g. pUC-derivatives can be used. Should however whole plants be regenerated from such transformed cells the presence of a selectable marker gene is necessary. The transformed cells grow within the plants in the usual manner (see also McCormick et al.(1986) Plant Cell Reports 5: 81-84). These plants can be grown normally and crossed with plants, that possess the same transformed genes or different genes. The resulting hybrid individuals have the corresponding phenotypical properties.

In order to understand the examples forming the basis of this invention all the processes necessary for these tests and which are known per se will first of all be listed:

1. Cloning process

The vectors pUC 18/19 and M13mp10 series (Yanisch-Perron et al. (1985) Gene 33: 103-119), as well as the vector EMBL 3 (Frischauf et al. (1983) J Mol Biol 170: 827-842) were used for cloning.

For the plant transformations, the gene constructs were cloned in the binary vector BIN 19 (Bevan (1984) Nucl. Acids Res 12: 8711-8720)

2. Bacterial strains

The E. coli strain BMH71-18 (Messing et al., Proc. Natl. Acad. Sci. USA (1977), 24, 6342-6346) or TB1 was used for the pUC and M13 mP vectors.

For the vector BIN19, the E. coli strain TB1 exclusively, was used. TB1 is a recombinant-negative, tetracycline-resistant derivative of strain JM101 (Yanisch-Perron et al., Gene (1985), 33, 103-119). The genotype of the TB1 strain is (Bart Barrel, personal communication): F' (traD36, proAB, lacI, lacZ.DELTA.M15), .DELTA.(lac, pro), SupE, this, recA, Sr1::Tn10(TcR).

The transformation of the plasmids into the potato plants was carried out using Agrobacterium tumefaciens strain LBA4404 (Bevan, (1934), Nucl. Acids Res. 12, 8711-8720).

TransporTation of Agrobacterium tumefaciens

In the case of BIN19 derivatives, the insertion of the DNA into the Agrobacterium was effected by direct transformation in accordance with the method of Holsters et al., (1978) (Mol Gene Genet 163: 181-187). The plasmid DNA of the transformed Agrobacterium was isolated in accordance with the method of Birnboim and Doly (1979) (Nucl Acids Res 7: 1513-1523) and was analysed by gel electrophoresis after suitable restriction cleavage.

4. Sucrose phosphate-synthase activity test

The sucrose phosphate-synthase activity was determined according to the method of Siegel and Stitt (1990, Plant Science 66: 205-210) in a two stage analysis. To 180 .mu.l of a solution of 50 mM HEPES/KOH (pH 7.4), 5 mM magnesium chloride, 5 mM fructose-6-phosphate, 25 mM glucose-6-phosphate and 6 mM uridine-5'-diphosphoglucose, 20 .mu.l of probe was added and incubated for 10 minutes at 25.degree. C. It was heated for 3 minutes at 95.degree. C., to complete the reaction. After centrifuging, the supernatant was spectroscopically analysed for the liberation of uridine-5'-diphosphate, whereby a pyruvate-kinase coupling enzyme reaction was used. Preparations without hexose phosphate, as well as the measurement of the recovery of added uridine-5'-diphosphate act as controls.

Cloning of cDNA to large and small subunits of the ADP glucose pyrophosphorylase of sugar beet.

From the storage roots of 3-4 month old sugar beet plants grown in the greenhouse, RNA was isolated according to the method of Logemann et al (1987, Anal Biochem 163, 16-20). Resulting from poly-A+-RNA, a cDNA library was laid down according to the method of Gubler and Hoffmann (1983, Gene 25, 263) in the expression vector Lambda Zap II XR. To this there was used an oligo-dT primer provided with an XhoI recognising position and for synthesis of the first cDNA strand, methylated cytidine nucleotide was inserted. After synthesis of the two strands, an EcoRI-adaptor was attached and removed to one side by again cutting with the restriction endonuclease XhoI. In this way the hemimethylation of cDNA was hindered so that an internal XhoI recognition position is cut. By these procedures there is obtained a population of cDNA molecules that can be cloned directly into the EcoRI/XhoI cut DNA of the phage Lambda. After packing of recombinant phage-DNA in phage heads, 200000 plaque forming units of the bank were plated out for infection of a bacterial colony and then each is probed with the total cDNA fragment of the large and/or subunit of the AGPase of potato (Muller-Roeber et al., 1990, MGG 224, 136-146) as an EcoRI-fragment. The recombinant phages corresponding to the hybridising signal were isolated. By in vivo excision, plasmids were cut out from the Lambda zap-genome, which carry a double stranded cDNA as insertion. The plasmids were transformed in bacterial cells. The plasmid-DNA was then propagated in the bacteria. After checking the size of the insertions, individual clones were analysed by determination of the primary sequence.

Cloning of cDNA to sucrose-phosphate-synthase (SPS) from sugar beet.

From the storage roots of 3-4 month old sugar beet plant grown in the greenhouse, RNA was isolated according to the method of Logemann et al (1987, Anal Biochem 163, 16-20). Resulting from poly-A+-RNA, a cDNA library was laid down according to the method of Gubler and Hoffmann (1983, Gene 25, 263) in the expression vector Lambda Zap II XR. To this there was used an oligo-dT primer provided with an XhoI recognising position and for synthesis of the first cDNA strand, methylated cytidine nucleotide was inserted. After synthesis of the two strands, an EcoRI-adaptor was attached and removed to one side by again cutting with the restriction endonuclease XhoI. In this way the hemimethylation of cDNA was hindered so that an internal XhoI recognition position is cut. By these procedures there is obtained a population of cDNA molecules that can be cloned directly into the EcoRI/XhoI cut DNA of the phage Lambda. After packing of recombinant phage-DNA in phage heads, 200000 plaque forming units of the bank were plated out for infection of a bacterial colony and then each is probed with the total cDNA fragment of the sucrose-phosphate-synthase (SPS) from spinach (Sonnewald, 1992, Planta) as NotI. The recombinant phages corresponding to the hybridising signal were isolated. By in vivo excision, plasmids were cut out from the Lambda zap-genome, which carry a double stranded cDNA as insertion. The plasmids were transformed in bacterial cells. The plasmid-DNA was then propagated in the bacteria. After checking the size of the insertions, individual clones were analysed by determination of the primary sequence.

Cloning of cDNA to sucrose-synthase from sugar beet.

From the storage roots of 3-4 month old sugar beet plants grown in the greenhouse, RNA was isolated according to the method of Logemann et al (1987, Anal Biochem 163, 16-20). Resulting from poly-A+-RNA, a cDNA library was laid down according to the method of Gubler and Hoffmann (1983, Gene 25, 263) in the expression vector Lambda Zap II XR. To this there was used an oligo-dT primer provided with an XhoI recognising position and for synthesis of the first cDNA strand, methylated cytidine nucleotide was inserted. After synthesis of the two strands, an EcoRI-adaptor was attached and removed to one side by again cutting with the restriction endonuclease XhoI. In this way the hemimethylation of cDNA was hindered so that an internal XhoI recognition position is cut. By these procedures there is obtained a population of cDNA molecules that can be cloned directly into the EcoRI/XhoI cut DNA of the phage Lambda. After packing of recombinant phage-DNA in phage heads, 200000 plaque forming units of the bank were plated out for infection of a bacterial colony and then each is probed with both EcoRI/BgIII sub fragments sucrose synthase from maize (Worrell et al., 1991, Plant Cell 3, 1121-1130). The recombinant phages corresponding to the hybridising signal were isolated. By in vivo excision, plasmids were cut out from the Lambda zap-genome, which carry a double stranded cDNA as insertion. The plasmids were transformed in bacterial cells. The plasmid-DNA was then propagated in the bacteria. After checking the size of the insertions, individual clones were analysed by determination of the primary sequence.

Determination of the nuclectide sequence of the ADP glucose pyrophosphorylase, the sucrose synthase and the sucrose phosohate synthase of sugar beet and derivation of the corresponding amino acid sequences

The nucleotide sequences of the instructions obtained from Examples 1-3, were determined by standard methods by means of the dideoxy method (Sanger et al. (1977) Proc. Natl. Acad. Sci. USA, 74, 5463-5467). The nucleotide sequences and the amino acid sequences derived therefrom are given in the sequence protocols Seq. ID No. 1-4.

The sequences are shown earlier; the protocols are as follows:

SEO ID NO: 1

SEO ID NO: 3

SEO ID NO: 5

SEO ID NO: 7 SEQUENCE LISTING (1) GENERAL INFORMATION: (iii) NUMBER OF SEQUENCES: 8 (2) INFORMATION FOR SEQ ID NO:1: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1925 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (vi) ORIGINAL SOURCE: (A) ORGANISM: Beta vulgaris (C) INDIVIDUAL ISOLATE: ADP Glucose Pyrophosphorylase, large subunit (vii) IMMEDIATE SOURCE: (A) LIBRARY: phage lamda zap (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 206..1774 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1: CAAAAGAAAAACTTCCCATTTCTACTTCTTTGCACAATATAATTTCCCACCAATTTTTCT60 TTAAATTTCTCACTTTCATTTAATCAGTTTTCAGCAACATTCTGATACTCGACAACCCAC120 TTTCTGTTCTCCCAAGATTCCAAACCTCTGATTCTCATTCCACTAATATTTTTGCTTATT180 TTTTTTCTGGATTTAAAGAAAAGCTATGGATGCAAGTGCAGCAGCCATAAAT232 MetAspAlaSerAlaAlaAlaIleAsn 15 GTCAATGCCCATTTAACAGAAGTTGGAAAGAAACGTTTTTTAGGAGAG280 ValAsnAlaHisLeuThrGluValGlyLysLysArgPheLeuGlyGlu 10152025 AGAATCAGTCAAAGTTTGAAGGGTAAAGATCTGAGAGCTCTGTTTTCA328 ArgIleSerGlnSerLeuLysGlyLysAspLeuArgAlaLeuPheSer 303540 AGAACTGAGAGCAAGGGTAGAAATGTCAATAAACCTGGGGTTGCATTT376 ArgThrGluSerLysGlyArgAsnValAsnLysProGlyValAlaPhe 455055 TCTGTTCTCACCTCAGATTTTAATCAAAGTGTTAAAGAATCTTTGAAA424 SerValLeuThrSerAspPheAsnGlnSerValLysGluSerLeuLys 606570 TATGAGCCAGCATTATTTGAATCTCCAAAAGCTGACCCAAAAAATGTG472 TyrGluProAlaLeuPheGluSerProLysAlaAspProLysAsnVal 758085 GCTGCAATTGTGCTGGGTGGTGGTGCTGGGACTCGCCTCTTTCCTCTT520 AlaAlaIleValLeuGlyGlyGlyAlaGlyThrArgLeuPheProLeu 9095100105 ACTAGCAGGAGAGCTAAGCCAGCAGTGCCAATTGGAGGGTGTTACAGG568 ThrSerArgArgAlaLysProAlaValProIleGlyGlyCysTyrArg 110115120 CTGATTGATGTGCCTATGAGCAACTGCATCAACAGTGGCATTAGAAAG616 LeuIleAspValProMetSerAsnCysIleAsnSerGlyIleArgLys 125130135 ATTTTCATTCTTACCCAGTTCAATTCGTTTTCGCTTAATCGTCATCTT664 IlePheIleLeuThrGlnPheAsnSerPheSerLeuAsnArgHisLeu 140145150 GCTCGAACCTATAATTTTGGAGATGGTGTGAATTTTGGGGATGGCTTT712 AlaArgThrTyrAsnPheGlyAspGlyValAsnPheGlyAspGlyPhe 155160165 GTGGAGGTTTTTGCTGCTACACAAACACCTGGAGAATCAGGAAAGAAA760 ValGluValPheAlaAlaThrGlnThrProGlyGluSerGlyLysLys 170175180185 TGGTTCCAGGGCACCGCTGATGCAGTAAGACAGTTTTTCTGGGCATTT808 TrpPheGlnGlyThrAlaAspAlaValArgGlnPhePheTrpAlaPhe 190195200 GAGGATTCCAAATCCAAGGATGTCGAGCATATAGTTATTTTATCCGGT856 GluAspSerLysSerLysAspValGluHisIleValIleLeuSerGly 205210215 GATCATCTTTACCGAATGGATTACATGAGTTTTTGGCAGAAGCACATT904 AspHisLeuTyrArgMetAspTyrMetSerPheTrpGlnLysHisIle 220225230 GACACCAATGCTGATATTACAGTGTCATGCATACCCATGGATGACAGC952 AspThrAsnAlaAspIleThrValSerCysIleProMetAspAspSer 235240245 CGTGCATCGGATTATGGGCTGATGAAGATTGATCACACTGGACGCATT1000 ArgAlaSerAspTyrGlyLeuMetLysIleAspHisThrGlyArgIle 250255260265 GTCCATTTTGCAGAAAAACCCAAGGGTTCTGATCTAACAGCAATGCAA1048 ValHisPheAlaGluLysProLysGlySerAspLeuThrAlaMetGln 270275280 GTAGATACAACTGTTCTTGGGCTCTCTGACCTTGAAGCTATGTCAAAT1096 ValAspThrThrValLeuGlyLeuSerAspLeuGluAlaMetSerAsn 285290295 CCATATATTGCATCAATGGGTGTTTATGTCTTTCGAACGGATGTTCTT1144 ProTyrIleAlaSerMetGlyValTyrValPheArgThrAspValLeu 300305310 ATGGAGCTTCTCAATCGAAAATACCCTTCAAGCAATGATTTTGGCTCT1192 MetGluLeuLeuAsnArgLysTyrProSerSerAsnAspPheGlySer 315320325 GAGATTATTCCTTCAGCTGTAGGAGAGTCTAATGTTCAGGCATATCTA1240 GluIleIleProSerAlaValGlyGluSerAsnValGlnAlaTyrLeu 330335340345 TTTAATGACTACTGGGAGGATATCGGAACCATAAAGTCTTTCTTTGAT1288 PheAsnAspTyrTrpGluAspIleGlyThrIleLysSerPhePheAsp 350355360 TCCAATTTGGCCCTTACACAACAGCCTCCCAAGTTTGAATTCTACGAT1336 SerAsnLeuAlaLeuThrGlnGlnProProLysPheGluPheTyrAsp 365370375 CCAAAAACACCTTTTTATACATCTGCAAGATTTCTGCCTCCTACAAAA1384 ProLysThrProPheTyrThrSerAlaArgPheLeuProProThrLys 380385390 GTCGACAGGTGCAAGATTGTCGATTCCATTGTATCCCATGGTTGTTTT1432 ValAspArgCysLysIleValAspSerIleValSerHisGlyCysPhe 395400405 CTACAGGAGTCTAGCATCCAACATTCCATTGTTGGTGTTCGCTCAAGA1480 LeuGlnGluSerSerIleGlnHisSerIleValGlyValArgSerArg 410415420425 TTAGAGTCCGGGGTTGAGTTCCAGGACACCATGATGATGGGCGCAGAT1528 LeuGluSerGlyValGluPheGlnAspThrMetMetMetGlyAlaAsp 430435440 TACTATCAAACTGAATCAGAAATTGCTTCTCTGCTTGCTGAGGGAAAG1576 TyrTyrGlnThrGluSerGluIleAlaSerLeuLeuAlaGluGlyLys 445450455 GTTCCTGTTGGTGTCGGACAGAATACCAAAATAAAGAATTGCATAATT1624 ValProValGlyValGlyGlnAsnThrLysIleLysAsnCysIleIle 460465470 GACAAGAACGCCAAAATTGGAAAAGATGTGGTAATCGCAAACACGGAT1672 AspLysAsnAlaLysIleGlyLysAspValValIleAlaAsnThrAsp 475480485 GGTGTTGAGGAAGCAGATAGACCAAATGAAGGCTTTTACATCAGGTCG1720 GlyValGluGluAlaAspArgProAsnGluGlyPheTyrIleArgSer 490495500505 GGCATTACCATCATTTTGAAGAACGCAACCATACAAGACGGTCTTGTG1768 GlyIleThrIleIleLeuLysAsnAlaThrIleGlnAspGlyLeuVal 510515520 ATTTAGATTTAATCATAACCTCATTAGAAAGAAATAATTTTGCATGATTTCCTTTT1824 Ile* CATGTAACCTAAACTGGCTAAACCACGAGGTTTTCTCATCTGTATATATAATATGTCTAT1884 AACTATGGATAATCTTAATAAAAAAAAAAAAAAAAAAAAAA1925 (2) INFORMATION FOR SEQ ID NO:2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 522 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2: MetAspAlaSerAlaAlaAlaIleAsnValAsnAlaHisLeuThrGlu 151015 ValGlyLysLysArgPheLeuGlyGluArgIleSerGlnSerLeuLys 202530 GlyLysAspLeuArgAlaLeuPheSerArgThrGluSerLysGlyArg 354045 AsnValAsnLysProGlyValAlaPheSerValLeuThrSerAspPhe 505560 AsnGlnSerValLysGluSerLeuLysTyrGluProAlaLeuPheGlu 65707580 SerProLysAlaAspProLysAsnValAlaAlaIleValLeuGlyGly 859095 GlyAlaGlyThrArgLeuPheProLeuThrSerArgArgAlaLysPro 100105110 AlaValProIleGlyGlyCysTyrArgLeuIleAspValProMetSer 115120125 AsnCysIleAsnSerGlyIleArgLysIlePheIleLeuThrGlnPhe 130135140 AsnSerPheSerLeuAsnArgHisLeuAlaArgThrTyrAsnPheGly 145150155160 AspGlyValAsnPheGlyAspGlyPheValGluValPheAlaAlaThr 165170175 GlnThrProGlyGluSerGlyLysLysTrpPheGlnGlyThrAlaAsp 180185190 AlaValArgGlnPhePheTrpAlaPheGluAspSerLysSerLysAsp 195200205 ValGluHisIleValIleLeuSerGlyAspHisLeuTyrArgMetAsp 210215220 TyrMetSerPheTrpGlnLysHisIleAspThrAsnAlaAspIleThr 225230235240 ValSerCysIleProMetAspAspSerArgAlaSerAspTyrGlyLeu 245250255 MetLysIleAspHisThrGlyArgIleValHisPheAlaGluLysPro 260265270 LysGlySerAspLeuThrAlaMetGlnValAspThrThrValLeuGly 275280285 LeuSerAspLeuGluAlaMetSerAsnProTyrIleAlaSerMetGly 290295300 ValTyrValPheArgThrAspValLeuMetGluLeuLeuAsnArgLys 305310315320 TyrProSerSerAsnAspPheGlySerGluIleIleProSerAlaVal 325330335 GlyGluSerAsnValGlnAlaTyrLeuPheAsnAspTyrTrpGluAsp 340345350 IleGlyThrIleLysSerPhePheAspSerAsnLeuAlaLeuThrGln 355360365 GlnProProLysPheGluPheTyrAspProLysThrProPheTyrThr 370375380 SerAlaArgPheLeuProProThrLysValAspArgCysLysIleVal 385390395400 AspSerIleValSerHisGlyCysPheLeuGlnGluSerSerIleGln 405410415 HisSerIleValGlyValArgSerArgLeuGluSerGlyValGluPhe 420425430 GlnAspThrMetMetMetGlyAlaAspTyrTyrGlnThrGluSerGlu 435440445 IleAlaSerLeuLeuAlaGluGlyLysValProValGlyValGlyGln 450455460 AsnThrLysIleLysAsnCysIleIleAspLysAsnAlaLysIleGly 465470475480 LysAspValValIleAlaAsnThrAspGlyValGluGluAlaAspArg 485490495 ProAsnGluGlyPheTyrIleArgSerGlyIleThrIleIleLeuLys 500505510 AsnAlaThrIleGlnAspGlyLeuValIle 515520 (2) INFORMATION FOR SEQ ID NO:3: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1763 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (vi) ORIGINAL SOURCE: (A) ORGANISM: Beta vulgaris (C) INDIVIDUAL ISOLATE: ADP Glucose Pyrophosphorylase, small subunit (vii) IMMEDIATE SOURCE: (A) LIBRARY: phage lamda zap (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 3..1472 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3: GGATAACTGTGCCATCAACCTCCTCAAAGAACCTCCAAAATAGCCTC47 IleThrValProSerThrSerSerLysAsnLeuGlnAsnSerLeu 151015 GCATTCTCCTCTTCTTCTCTCTCCGGCGACAAAATTCAAACGACGTCA95 AlaPheSerSerSerSerLeuSerGlyAspLysIleGlnThrThrSer 202530 TTTCTCAACCGCCGATATTGTAGAATCTCTTCTAGAGCTCCGATTGTT143 PheLeuAsnArgArgTyrCysArgIleSerSerArgAlaProIleVal 354045 GTCTCTCCCAAAGCTGTTTCTGATTCTAAGAATTCGCAGACTTGTCTT191 ValSerProLysAlaValSerAspSerLysAsnSerGlnThrCysLeu 505560 GACCCTGAAGCCAGCCGTAGTGTTCTTGGTATTATACTTGGAGGTGGT239 AspProGluAlaSerArgSerValLeuGlyIleIleLeuGlyGlyGly 657075 GCTGGTACACGTCTTTACCCGTTGACTAAGAAGAGAGCCAAACCAGCC287 AlaGlyThrArgLeuTyrProLeuThrLysLysArgAlaLysProAla 80859095 GTGCCACTCGGTGCTAATTATAGGCTTATTGATATCCCAGTGAGCAAT335 ValProLeuGlyAlaAsnTyrArgLeuIleAspIleProValSerAsn 100105110 TGTTTGAACAGTAATATTTCCAAAATATATGTTCTTACACAATTCAAT383 CysLeuAsnSerAsnIleSerLysIleTyrValLeuThrGlnPheAsn 115120125 TCTGCTTCTCTGAATCGTCATCTTTCGCGGGCATATGCTAGCAACATG431 SerAlaSerLeuAsnArgHisLeuSerArgAlaTyrAlaSerAsnMet 130135140 GGAGGATACAAAAATGAGGGGTTTGTAGAAGTTCTTGCTGCTCAGCAA479 GlyGlyTyrLysAsnGluGlyPheValGluValLeuAlaAlaGlnGln 145150155 AGTCCAGAGAATCCAAACTGGTTTCAGGGTACAGCTGATGCTGTTAGG527 SerProGluAsnProAsnTrpPheGlnGlyThrAlaAspAlaValArg 160165170175 CAATATCTGTGGCTTTTCGAAGAGCACAATGTTCTTGAGTACTTGATT575 GlnTyrLeuTrpLeuPheGluGluHisAsnValLeuGluTyrLeuIle 180185190 CTTGCTGGTGACCATTTGTATCGAATGGATTATGAAAGATTTGTCCAA623 LeuAlaGlyAspHisLeuTyrArgMetAspTyrGluArgPheValGln 195200205 GCTCACAGAGAAACTGATGCAGACATTACTGTTGCTGCATTGCCAATG671 AlaHisArgGluThrAspAlaAspIleThrValAlaAlaLeuProMet 210215220 GATGAAAAGCGTGCTACTGCATTTGGTTTGATGAAAATTGATGAAGAA719 AspGluLysArgAlaThrAlaPheGlyLeuMetLysIleAspGluGlu 225230235 GGAAGAATTATTGAGTTTGCCGAGAAACCGAAAGGAGAACAATTGAAA767 GlyArgIleIleGluPheAlaGluLysProLysGlyGluGlnLeuLys 240245250255 GCTATGAAGGTTGATACCACAATCCTGGGTCTGGACGATGAGAGAGCA815 AlaMetLysValAspThrThrIleLeuGlyLeuAspAspGluArgAla 260265270 AAAGAAATGCCATTCATAGCCAGCATGGGCATATATGTTATTAGCAAA863 LysGluMetProPheIleAlaSerMetGlyIleTyrValIleSerLys 275280285 GATGTAATGCTTAATCTGCTTCGGGAGCAATTTCCTGGTGCTAATGAT911 AspValMetLeuAsnLeuLeuArgGluGlnPheProGlyAlaAsnAsp 290295300 TTTGGAAGTGAAGTTATTCCAGGCGCCACTTCCATAGGGTTGAGAGTC959 PheGlySerGluValIleProGlyAlaThrSerIleGlyLeuArgVal 305310315 CAAGCTTATTTGTATGATGGTTACTGGGAGGATATTGGTACCATTGAA1007 GlnAlaTyrLeuTyrAspGlyTyrTrpGluAspIleGlyThrIleGlu 320325330335 GCTTTTTACAATGCTAACTTGGGAATCACCAAAAAGCCGGTGCCAGAT1055 AlaPheTyrAsnAlaAsnLeuGlyIleThrLysLysProValProAsp 340345350 TTTAGCTTCTATGATCGTTCATCTCCAATTTATACACAACCTCGGTAT1103 PheSerPheTyrAspArgSerSerProIleTyrThrGlnProArgTyr 355360365 TTGCCTCCTTCAAAGATGCTTGATGCTGATATAACTGACAGCGTCATC1151 LeuProProSerLysMetLeuAspAlaAspIleThrAspSerValIle 370375380 GGTGAAGGCTGTGTTATTAAGAACTGTAAGATTCATCATTCTGTTATC1199 GlyGluGlyCysValIleLysAsnCysLysIleHisHisSerValIle 385390395 GGACTTCGATCTTGTATCTCGGAGGGTGCAATCATTGAGGACACACTG1247 GlyLeuArgSerCysIleSerGluGlyAlaIleIleGluAspThrLeu 400405410415 TTGATGGGAGCTGATTATTATGAGACTGATGCTGATCGGAAATTCCTG1295 LeuMetGlyAlaAspTyrTyrGluThrAspAlaAspArgLysPheLeu 420425430 GCTGCTAAGGGTAGTGTACCTATTGGAATTGGGAATGCACGTATTGGG1343 AlaAlaLysGlySerValProIleGlyIleGlyAsnAlaArgIleGly 435440445 GATGATGTCAAGATTATCAACAGTGACAATGTACAAGAAGCAGCAAGA1391 AspAspValLysIleIleAsnSerAspAsnValGlnGluAlaAlaArg 450455460 GAAACAGACGGATACTTCATAAAGAGCGGAATAGTCACTATAATCAAG1439 GluThrAspGlyTyrPheIleLysSerGlyIleValThrIleIleLys 465470475 GACGCCATGATTCCAAGTGGAACTGTAATCTAGAAATGGAGCATATAATAAAT1492 AspAlaMetIleProSerGlyThrValIle* 480485490 ATCACTGCCTATTTACAGTACCTATCTGAGTCTCCCACCATGACCCTTTGATTCAATCTT1552 TTAGTTATGTAAATATTTTTGGCTTTTGCGATTTTGCCATAAATTTGAAGAAGCGAGGAT1612 TCAGGGACGATAGTGCTATGAATTGGAAGAAAGGATTTGGGGGATATCTTTGTAAAGACA1672 TTTTGACTACTGGGCACTAAAAATTTGGTAATGCTATACCAAAATATATAAAAAGATCTT1732 GCTGGGTTTTGGTAAAAAAAAAAAAAAAAAA1763 (2) INFORMATION FOR SEQ ID NO:4: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 489 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4: IleThrValProSerThrSerSerLysAsnLeuGlnAsnSerLeuAla 151015 PheSerSerSerSerLeuSerGlyAspLysIleGlnThrThrSerPhe 202530 LeuAsnArgArgTyrCysArgIleSerSerArgAlaProIleValVal 354045 SerProLysAlaValSerAspSerLysAsnSerGlnThrCysLeuAsp 505560 ProGluAlaSerArgSerValLeuGlyIleIleLeuGlyGlyGlyAla 65707580 GlyThrArgLeuTyrProLeuThrLysLysArgAlaLysProAlaVal 859095 ProLeuGlyAlaAsnTyrArgLeuIleAspIleProValSerAsnCys 100105110 LeuAsnSerAsnIleSerLysIleTyrValLeuThrGlnPheAsnSer 115120125 AlaSerLeuAsnArgHisLeuSerArgAlaTyrAlaSerAsnMetGly 130135140 GlyTyrLysAsnGluGlyPheValGluValLeuAlaAlaGlnGlnSer 145150155160 ProGluAsnProAsnTrpPheGlnGlyThrAlaAspAlaValArgGln 165170175 TyrLeuTrpLeuPheGluGluHisAsnValLeuGluTyrLeuIleLeu 180185190 AlaGlyAspHisLeuTyrArgMetAspTyrGluArgPheValGlnAla 195200205 HisArgGluThrAspAlaAspIleThrValAlaAlaLeuProMetAsp 210215220 GluLysArgAlaThrAlaPheGlyLeuMetLysIleAspGluGluGly 225230235240 ArgIleIleGluPheAlaGluLysProLysGlyGluGlnLeuLysAla 245250255 MetLysValAspThrThrIleLeuGlyLeuAspAspGluArgAlaLys 260265270 GluMetProPheIleAlaSerMetGlyIleTyrValIleSerLysAsp 275280285 ValMetLeuAsnLeuLeuArgGluGlnPheProGlyAlaAsnAspPhe 290295300 GlySerGluValIleProGlyAlaThrSerIleGlyLeuArgValGln 305310315320 AlaTyrLeuTyrAspGlyTyrTrpGluAspIleGlyThrIleGluAla 325330335 PheTyrAsnAlaAsnLeuGlyIleThrLysLysProValProAspPhe 340345350 SerPheTyrAspArgSerSerProIleTyrThrGlnProArgTyrLeu 355360365 ProProSerLysMetLeuAspAlaAspIleThrAspSerValIleGly 370375380 GluGlyCysValIleLysAsnCysLysIleHisHisSerValIleGly 385390395400 LeuArgSerCysIleSerGluGlyAlaIleIleGluAspThrLeuLeu 405410415 MetGlyAlaAspTyrTyrGluThrAspAlaAspArgLysPheLeuAla 420425430 AlaLysGlySerValProIleGlyIleGlyAsnAlaArgIleGlyAsp 435440445 AspValLysIleIleAsnSerAspAsnValGlnGluAlaAlaArgGlu 450455460 ThrAspGlyTyrPheIleLysSerGlyIleValThrIleIleLysAsp 465470475480 AlaMetIleProSerGlyThrValIle 485 (2) INFORMATION FOR SEQ ID NO:5: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 3635 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (vi) ORIGINAL SOURCE: (A) ORGANISM: Beta vulgaris (C) INDIVIDUAL ISOLATE: Saccharosephosphate Synthase (vii) IMMEDIATE SOURCE: (A) LIBRARY: phage lamda zap (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 30..3167 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5: GGGCTGCAGGGAAGCTCTGAACTTCAAAAATGGCGGGAAATGATTGGATAAAC53 MetAlaGlyAsnAspTrpIleAsn 15 AGTTATTTAGAGGCAATTCTGGATGTGGGTCCAGGACTTGATGATGCA101 SerTyrLeuGluAlaIleLeuAspValGlyProGlyLeuAspAspAla 101520 AAATCATCTTTGCTTTTGAGAGAAAGAGGCAGGTTTAGTCCTACTCGT149 LysSerSerLeuLeuLeuArgGluArgGlyArgPheSerProThrArg 25303540 TACTTTGTTGAAGAAGTTATCACTGGTTTTGATGAAACCGACCTTCAT197 TyrPheValGluGluValIleThrGlyPheAspGluThrAspLeuHis 455055 CGTTCATGGGTTCGGGCACAAGCAACAAGGAGTCCTCAAGAGAGGAAT245 ArgSerTrpValArgAlaGlnAlaThrArgSerProGlnGluArgAsn 606570 ACTAGATTGGAGAACATGTGTTGGAGAATTTGGAATTTGGCTCGTCAG293 ThrArgLeuGluAsnMetCysTrpArgIleTrpAsnLeuAlaArgGln 758085 AAGAAGCAGCTTGAGAATGAAGAAGCTCAGCGGAAGACAAAACGTCGT341 LysLysGlnLeuGluAsnGluGluAlaGlnArgLysThrLysArgArg 9095100 ATGGAGCTTGAGAGGGGTCGTCGAGAAGCAACTGCTGATATGTCGGAG389 MetGluLeuGluArgGlyArgArgGluAlaThrAlaAspMetSerGlu 105110115120 GACTTATCAGAAGGCGAAAAGGACATTTCAGCTCATGGTGATAGCACC437 AspLeuSerGluGlyGluLysAspIleSerAlaHisGlyAspSerThr 125130135 CGTCCTAGATTGCCAAGAATAAATTCTCTTGATGCTATGGAGACATGG485 ArgProArgLeuProArgIleAsnSerLeuAspAlaMetGluThrTrp 140145150 ATTAGTCAACAAAAGGAAAAAAAACTCTACCTTGTTTTGATAAGTCTT533 IleSerGlnGlnLysGluLysLysLeuTyrLeuValLeuIleSerLeu 155160165 CATGGTTTGATACGAGGTGAAAACATGGAACTTGGCCGTGATTCTGAT581 HisGlyLeuIleArgGlyGluAsnMetGluLeuGlyArgAspSerAsp 170175180 ACTGGTGGTCAGGTTAAGTATGTGGTTGAGCTTGCAAGGGCTCTAGGT629 ThrGlyGlyGlnValLysTyrValValGluLeuAlaArgAlaLeuGly 185190195200 TCGATGCCAGGTGTTTATAGAGTTGATTTGCTAACTAGGCAAGTTTCA677 SerMetProGlyValTyrArgValAspLeuLeuThrArgGlnValSer 205210215 TCTCCTGACGTGGATTGGAGTTATGGGGAGCCTACTGAGATGCTGAAT725 SerProAspValAspTrpSerTyrGlyGluProThrGluMetLeuAsn 220225230 CCAAGGGATTCCAATGGTTTTGATGATGATGATGATGAAATGGGAGAG773 ProArgAspSerAsnGlyPheAspAspAspAspAspGluMetGlyGlu 235240245 AGTAGTGGTGCTTACATTGTTCGTATACCATTTGGGCCGAGGGATAAG821 SerSerGlyAlaTyrIleValArgIleProPheGlyProArgAspLys 250255260 TATATCGCAAAAGAAGAGCTTTGGCCCTATATTCCTGAATTTGTTGAT869 TyrIleAlaLysGluGluLeuTrpProTyrIleProGluPheValAsp 265270275280 GGTGCTCTAAACCACATAGTTCAAATGTCCAAAGTTTTAGGTGAGCAA917 GlyAlaLeuAsnHisIleValGlnMetSerLysValLeuGlyGluGln 285290295 ATTGGTAGCGGGGAAACAGTTTGGCCAGTTGCCATTCATGGACATTAT965 IleGlySerGlyGluThrValTrpProValAlaIleHisGlyHisTyr 300305310 GCTGATGCTGGTGATTCTGCTGCTCTTCTTTCTGGTGGCCTAAATGTT1013 AlaAspAlaGlyAspSerAlaAlaLeuLeuSerGlyGlyLeuAsnVal 315320325 CCAATGCTTTTAACGGGGCATTCTCTTGGCCGAGACAAGTTAGAGCAG1061 ProMetLeuLeuThrGlyHisSerLeuGlyArgAspLysLeuGluGln 330335340 CTCCTCAAACAGGGTCGAATGTCTAAAGATGACATAAACAATACATAC1109 LeuLeuLysGlnGlyArgMetSerLysAspAspIleAsnAsnThrTyr 345350355360 AAAATAATGCGTAGGATAGAAGCCGAAGAGTTATCACTTGATGCCTCT1157 LysIleMetArgArgIleGluAlaGluGluLeuSerLeuAspAlaSer 365370375 GAGATAGTCATAACTAGTACAAGACAAGAAATAGAAGAGCAATGGCAC1205 GluIleValIleThrSerThrArgGlnGluIleGluGluGlnTrpHis 380385390 CTCTATGATGGGTTTGATCCTGTGCTAGAACGTAAACTCCGTGCTAGG1253 LeuTyrAspGlyPheAspProValLeuGluArgLysLeuArgAlaArg 395400405 ATGAAGCGTGGTGTAAGCTGTTATGGAAGGTTCATGCCCCGGATGGTT1301 MetLysArgGlyValSerCysTyrGlyArgPheMetProArgMetVal 410415420 GTTATTCCTCCTGGAATGGAATTCAATCATATTGTTCCACATGAGGGT1349 ValIleProProGlyMetGluPheAsnHisIleValProHisGluGly 425430435440 GATATGGATGGTGAAACAGAAGAAACTGAAGAGCATCCTACATCACCT1397 AspMetAspGlyGluThrGluGluThrGluGluHisProThrSerPro 445450455 GATCCACCTATCTGGGCTGAGATTATGCGCTTCTTTTCTAAACCAAGG1445 AspProProIleTrpAlaGluIleMetArgPhePheSerLysProArg 460465470 AAGCCAATGATACTTGCCCTTGCTAGGCCTGACCCGAAGAAGAATATC1493 LysProMetIleLeuAlaLeuAlaArgProAspProLysLysAsnIle 475480485 ACGACTTTGGTCAAAGCATTTGGAGAATGCCGTCCACTAAGGGAGCTA1541 ThrThrLeuValLysAlaPheGlyGluCysArgProLeuArgGluLeu 490495500 GCTAATCTTACTCTTATAATGGGTAACCGAGATGGTATTGACGAGATG1589 AlaAsnLeuThrLeuIleMetGlyAsnArgAspGlyIleAspGluMet 505510515520 TCAAGCACCAGTTCTTCAGTTCTCCTGTCAGTGCTTAAGCTAATTGAT1637 SerSerThrSerSerSerValLeuLeuSerValLeuLysLeuIleAsp 525530535 CAATACGACCTTTATGGTCAAGTAGCATACCCCAAACATCACAAGCAA1685 GlnTyrAspLeuTyrGlyGlnValAlaTyrProLysHisHisLysGln 540545550 GCTGATGTTCCTGAGATTTATCGTTTGGCAGCAAAGACAAAGGGAGTC1733 AlaAspValProGluIleTyrArgLeuAlaAlaLysThrLysGlyVal 555560565 TTTATTAATCCAGCTTTTATTGAGCCATTTGGGCTGACTCTAATAGAG1781 PheIleAsnProAlaPheIleGluProPheGlyLeuThrLeuIleGlu 570575580 GCAGCAGCTCATGGTTTACCGATGGTTGCTACGAAAAATGGAGGCCCT1829 AlaAlaAlaHisGlyLeuProMetValAlaThrLysAsnGlyGlyPro 585590595600 GTTGATATCCAGAGGGTCCTTGATAATGGTCTTCTTGTGGATCCTCAT1877 ValAspIleGlnArgValLeuAspAsnGlyLeuLeuValAspProHis 605610615 GAGCAGCAGTCTATTGCTACTGCTTTGCTGAAGCTTGTTGCTGATAAG1925 GluGlnGlnSerIleAlaThrAlaLeuLeuLysLeuValAlaAspLys 620625630 CAACTATGGACAAAATGCCAGCAAAATGGACTGAAAAATATTCATCTC1973 GlnLeuTrpThrLysCysGlnGlnAsnGlyLeuLysAsnIleHisLeu 635640645 TACTCTTGGCCAGAGCATTCGAAGACATACCTATCTCGAATAGCCAGT2021 TyrSerTrpProGluHisSerLysThrTyrLeuSerArgIleAlaSer 650655660 TCGAGACAAAGGCAACCACAGTGGCAAAGAAGTAGTGATGAAGGGCTT2069 SerArgGlnArgGlnProGlnTrpGlnArgSerSerAspGluGlyLeu 665670675680 GACAATCAAGAGCCTGAATCTCCAAGTGATTCTTTAAGAGATATAAAG2117 AspAsnGlnGluProGluSerProSerAspSerLeuArgAspIleLys 685690695 GATATATCTCTAAACCTTGAAGTTCTCGTTAGACCGGAGAAAAGGGTG2165 AspIleSerLeuAsnLeuGluValLeuValArgProGluLysArgVal 700705710 AAGACGTTGAAAATCTTGGGATTGATGACAAAAGCAAATTCGAGAATG2213 LysThrLeuLysIleLeuGlyLeuMetThrLysAlaAsnSerArgMet 715720725 CTGTTATGTTCATGGTCTAATGGTGTCCATAAGATGCTTCGGAAGGCT2261 LeuLeuCysSerTrpSerAsnGlyValHisLysMetLeuArgLysAla 730735740 CGGTTCTCTGACAAAGTAGATCAGGCTTCTAGTAAATATCCAGCATTT2309 ArgPheSerAspLysValAspGlnAlaSerSerLysTyrProAlaPhe 745750755760 AGGAGGAGAAAACTTATATATGTTATTGCTGTAGACGGGGATTATGAA2357 ArgArgArgLysLeuIleTyrValIleAlaValAspGlyAspTyrGlu 765770775 GATGGACTTTTTGATATTGTTCGGAGGATATTTGATGCTGCTGGCAAG2405 AspGlyLeuPheAspIleValArgArgIlePheAspAlaAlaGlyLys 780785790 GAGAAGATTGAAGGTTCCATCGGGTTTATATTGTCAACATCCTATTCT2453 GluLysIleGluGlySerIleGlyPheIleLeuSerThrSerTyrSer 795800805 ATGCCCGAAATTCAGAACTATTTGCTATCAAAAGGCTTCAATCTTCAT2501 MetProGluIleGlnAsnTyrLeuLeuSerLysGlyPheAsnLeuHis 810815820 GATTTTGATGCATATATATGCAACAGTGGGAGTGAGTTGTACTATTCA2549 AspPheAspAlaTyrIleCysAsnSerGlySerGluLeuTyrTyrSer 825830835840 TCTTTGAACTCAGAGGAGAGTAATATTATAGCAGATTCAGATTACCAT2597 SerLeuAsnSerGluGluSerAsnIleIleAlaAspSerAspTyrHis 845850855 TCACACATAGAGTACAGATGGGGTGGAGAAGGCCTTAGAAGGACTTTG2645 SerHisIleGluTyrArgTrpGlyGlyGluGlyLeuArgArgThrLeu 860865870 CTTCGCTGGGCAGCTTCCATCACAGAAAAAAATGGTGAAAACGAAGAA2693 LeuArgTrpAlaAlaSerIleThrGluLysAsnGlyGluAsnGluGlu 875880885 CAGGTTATTACTGAAGATGAAGAAGTTTCTACGGGTTATTGCTTTGCG2741 GlnValIleThrGluAspGluGluValSerThrGlyTyrCysPheAla 890895900 TTTAAAATAAAGAACCAAAATAAGGTTCCCCCTACGAAGGAGCTCCGC2789 PheLysIleLysAsnGlnAsnLysValProProThrLysGluLeuArg 905910915920 AAGTCAATGAGGATTCAAGCTCTTCGTTGCCATGTGATTTACTGTCAG2837 LysSerMetArgIleGlnAlaLeuArgCysHisValIleTyrCysGln 925930935 AACGGATCTAAAATGAATGTGATTCCAGTACTAGCATCCCGTTCTCAA2885 AsnGlySerLysMetAsnValIleProValLeuAlaSerArgSerGln 940945950 GCCCTCAGGTATCTTTATGTTCGTTGGGGAGTTGAGTTGTCGAAGATG2933 AlaLeuArgTyrLeuTyrValArgTrpGlyValGluLeuSerLysMet 955960965 GTTGTCTTTGTTGGAGAATGTGGTGACACAGATTATGAAGGCTTGCTT2981 ValValPheValGlyGluCysGlyAspThrAspTyrGluGlyLeuLeu 970975980 GGCGGGGTCCATAAAACCGTAATACTGAAGGGAGTCTCCAACACTGCT3029 GlyGlyValHisLysThrValIleLeuLysGlyValSerAsnThrAla 9859909951000 TTAAGGTCTCTCCATGCCAACAGAAGTTACCCTCTTTCACATGTCGTG3077 LeuArgSerLeuHisAlaAsnArgSerTyrProLeuSerHisValVal 100510101015 TCGCTTGACAGCCCCAATATTGGCGAGGTGAGCAAAGGGTGCAGCAGC3125 SerLeuAspSerProAsnIleGlyGluValSerLysGlyCysSerSer 102010251030 TCCGAGATCCAGTCCATCGTCACAAAACTCTCCAAAGCTTAA3167 SerGluIleGlnSerIleValThrLysLeuSerLysAla* 103510401045 TCAGATATCTGCTGCTTTCTTTTGGGTAAGCAAGGTTTCATCTTATATGATTATATCATA3227 AGATACTATATAAGCACCTTATTGGTAAGTCAGTCCCATAATAATAATGTACTTCAGAAC3287 CACAATACTTAAAAGTTGGTTCAGTAGTGATTAGTCTCATAATAATCATATAATTACACA3347 TCCGCTGTTAACTAGTGGTAATATCTAAGCTCAACAATAAAGATGTAAAATGCTAGTATG3407 GAAATGAATTGCTAGCTGTTGATCTCTTTCCCTTTATTCTGTATTATTTCTTTCCTCATC3467 TCATGTAAAAACAATTTTCTGAAGGTGTACAGTTTTTTCCCCTTATATATCTGTATTATT3527 TCTACTATTTTTTGTTTGTAAGAATATCCTCTCATCGAGGAGTGATAATTAAATAACCGG3587 CTTGCTAAATATAAAGCTTATTCGAGTTAAAAAAAAAAAAAAAAAAAA3635 (2) INFORMATION FOR SEQ ID NO:6: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1045 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6: MetAlaGlyAsnAspTrpIleAsnSerTyrLeuGluAlaIleLeuAsp 151015 ValGlyProGlyLeuAspAspAlaLysSerSerLeuLeuLeuArgGlu 202530 ArgGlyArgPheSerProThrArgTyrPheValGluGluValIleThr 354045 GlyPheAspGluThrAspLeuHisArgSerTrpValArgAlaGlnAla 505560 ThrArgSerProGlnGluArgAsnThrArgLeuGluAsnMetCysTrp 65707580 ArgIleTrpAsnLeuAlaArgGlnLysLysGlnLeuGluAsnGluGlu 859095 AlaGlnArgLysThrLysArgArgMetGluLeuGluArgGlyArgArg 100105110 GluAlaThrAlaAspMetSerGluAspLeuSerGluGlyGluLysAsp 115120125 IleSerAlaHisGlyAspSerThrArgProArgLeuProArgIleAsn 130135140 SerLeuAspAlaMetGluThrTrpIleSerGlnGlnLysGluLysLys 145150155160 LeuTyrLeuValLeuIleSerLeuHisGlyLeuIleArgGlyGluAsn 165170175 MetGluLeuGlyArgAspSerAspThrGlyGlyGlnValLysTyrVal 180185190 ValGluLeuAlaArgAlaLeuGlySerMetProGlyValTyrArgVal 195200205 AspLeuLeuThrArgGlnValSerSerProAspValAspTrpSerTyr 210215220 GlyGluProThrGluMetLeuAsnProArgAspSerAsnGlyPheAsp 225230235240 AspAspAspAspGluMetGlyGluSerSerGlyAlaTyrIleValArg 245250255 IleProPheGlyProArgAspLysTyrIleAlaLysGluGluLeuTrp 260265270 ProTyrIleProGluPheValAspGlyAlaLeuAsnHisIleValGln 275280285 MetSerLysValLeuGlyGluGlnIleGlySerGlyGluThrValTrp 290295300 ProValAlaIleHisGlyHisTyrAlaAspAlaGlyAspSerAlaAla 305310315320 LeuLeuSerGlyGlyLeuAsnValProMetLeuLeuThrGlyHisSer 325330335 LeuGlyArgAspLysLeuGluGlnLeuLeuLysGlnGlyArgMetSer 340345350 LysAspAspIleAsnAsnThrTyrLysIleMetArgArgIleGluAla 355360365 GluGluLeuSerLeuAspAlaSerGluIleValIleThrSerThrArg 370375380 GlnGluIleGluGluGlnTrpHisLeuTyrAspGlyPheAspProVal 385390395400 LeuGluArgLysLeuArgAlaArgMetLysArgGlyValSerCysTyr 405410415 GlyArgPheMetProArgMetValValIleProProGlyMetGluPhe 420425430 AsnHisIleValProHisGluGlyAspMetAspGlyGluThrGluGlu 435440445 ThrGluGluHisProThrSerProAspProProIleTrpAlaGluIle 450455460 MetArgPhePheSerLysProArgLysProMetIleLeuAlaLeuAla 465470475480 ArgProAspProLysLysAsnIleThrThrLeuValLysAlaPheGly 485490495 GluCysArgProLeuArgGluLeuAlaAsnLeuThrLeuIleMetGly 500505510 AsnArgAspGlyIleAspGluMetSerSerThrSerSerSerValLeu 515520525 LeuSerValLeuLysLeuIleAspGlnTyrAspLeuTyrGlyGlnVal 530535540 AlaTyrProLysHisHisLysGlnAlaAspValProGluIleTyrArg 545550555560 LeuAlaAlaLysThrLysGlyValPheIleAsnProAlaPheIleGlu 565570575 ProPheGlyLeuThrLeuIleGluAlaAlaAlaHisGlyLeuProMet 580585590 ValAlaThrLysAsnGlyGlyProValAspIleGlnArgValLeuAsp 595600605 AsnGlyLeuLeuValAspProHisGluGlnGlnSerIleAlaThrAla 610615620 LeuLeuLysLeuValAlaAspLysGlnLeuTrpThrLysCysGlnGln 625630635640 AsnGlyLeuLysAsnIleHisLeuTyrSerTrpProGluHisSerLys 645650655 ThrTyrLeuSerArgIleAlaSerSerArgGlnArgGlnProGlnTrp 660665670 GlnArgSerSerAspGluGlyLeuAspAsnGlnGluProGluSerPro 675680685 SerAspSerLeuArgAspIleLysAspIleSerLeuAsnLeuGluVal 690695700 LeuValArgProGluLysArgValLysThrLeuLysIleLeuGlyLeu 705710715720 MetThrLysAlaAsnSerArgMetLeuLeuCysSerTrpSerAsnGly 725730735 ValHisLysMetLeuArgLysAlaArgPheSerAspLysValAspGln 740745750 AlaSerSerLysTyrProAlaPheArgArgArgLysLeuIleTyrVal 755760765 IleAlaValAspGlyAspTyrGluAspGlyLeuPheAspIleValArg 770775780 ArgIlePheAspAlaAlaGlyLysGluLysIleGluGlySerIleGly 785790795800 PheIleLeuSerThrSerTyrSerMetProGluIleGlnAsnTyrLeu 805810815 LeuSerLysGlyPheAsnLeuHisAspPheAspAlaTyrIleCysAsn 820825830 SerGlySerGluLeuTyrTyrSerSerLeuAsnSerGluGluSerAsn 835840845 IleIleAlaAspSerAspTyrHisSerHisIleGluTyrArgTrpGly 850855860 GlyGluGlyLeuArgArgThrLeuLeuArgTrpAlaAlaSerIleThr 865870875880 GluLysAsnGlyGluAsnGluGluGlnValIleThrGluAspGluGlu 885890895 ValSerThrGlyTyrCysPheAlaPheLysIleLysAsnGlnAsnLys 900905910 ValProProThrLysGluLeuArgLysSerMetArgIleGlnAlaLeu 915920925 ArgCysHisValIleTyrCysGlnAsnGlySerLysMetAsnValIle 930935940 ProValLeuAlaSerArgSerGlnAlaLeuArgTyrLeuTyrValArg 945950955960 TrpGlyValGluLeuSerLysMetValValPheValGlyGluCysGly 965970975 AspThrAspTyrGluGlyLeuLeuGlyGlyValHisLysThrValIle 980985990 LeuLysGlyValSerAsnThrAlaLeuArgSerLeuHisAlaAsnArg 99510001005 SerTyrProLeuSerHisValValSerLeuAspSerProAsnIleGly 101010151020 GluValSerLysGlyCysSerSerSerGluIleGlnSerIleValThr 1025103010351040 LysLeuSerLysAla 1045 (2) INFORMATION FOR SEQ ID NO:7: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 2563 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) MOLECULE TYPE: cDNA (vi) ORIGINAL SOURCE: (A) ORGANISM: Beta vulgaris (C) INDIVIDUAL ISOLATE: Saccharosesynthase (vii) IMMEDIATE SOURCE: (A) LIBRARY: phage lamda zap (ix) FEATURE: (A) NAME/KEY: CDS (B) LOCATION: 3..2303 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7: CTGCAGGAGGGAAACAAATTCTTAGCGATGGCCCGTTTAGCGAAGTT47 AlaGlyGlyLysGlnIleLeuSerAspGlyProPheSerGluVal 151015 CTTAGGTCTGCTCAGGAAGCAATAGTTGTTCCTCCCTTTGTTGCTATA95 LeuArgSerAlaGlnGluAlaIleValValProProPheValAlaIle 202530 GCAGTCCGTCCAAGACCTGGAGTTTGGGAATATGTTCGTGTTAATGTC143 AlaValArgProArgProGlyValTrpGluTyrValArgValAsnVal 354045 TCTGAACTGAATGTGGAGCAGCTAACTGTGTCTGAGTATCTCCATTTC191 SerGluLeuAsnValGluGlnLeuThrValSerGluTyrLeuHisPhe 505560 AAGGAAGAACTTGTGGATGGAAAGGCTGATGACCACTATGTGCTTGAG239 LysGluGluLeuValAspGlyLysAlaAspAspHisTyrValLeuGlu 657075 CTTGATTTCGAGCCTTTTAATGAATCAGTTCCACGTCCAACTCGCTCT287 LeuAspPheGluProPheAsnGluSerValProArgProThrArgSer 80859095 TCATCAATTGGTAATGGTGTTCAGTTCCTCAATCGGCACCTGTCATCA335 SerSerIleGlyAsnGlyValGlnPheLeuAsnArgHisLeuSerSer 100105110 AGCATGTTCTGCAACAAAGATTGCTTGGAGCCGTTACTTGATTTTCTT383 SerMetPheCysAsnLysAspCysLeuGluProLeuLeuAspPheLeu 115120125 AGAGTGCACAAACATAAAGGAGTTGTCATGATGTTGAATGATCGGATA431 ArgValHisLysHisLysGlyValValMetMetLeuAsnAspArgIle 130135140 CAGACTATCCAGCGTCTTCAGTCTGCATTGTCTAAAGCTGAGGATTAT479 GlnThrIleGlnArgLeuGlnSerAlaLeuSerLysAlaGluAspTyr 145150155 CTTATCAAACTTCCAGCAGATACACCTTACTCTGAGTTCGAATTTGTA527 LeuIleLysLeuProAlaAspThrProTyrSerGluPheGluPheVal 160165170175 ATCCAAGGTATGGGTTTTGAAAGAGGCTGGGGTGATACTGCTGAAAGG575 IleGlnGlyMetGlyPheGluArgGlyTrpGlyAspThrAlaGluArg 180185190 GTTCTAGAAATGATGCATCTACTACTAGATATCCTTCAGGCTCCCGAT623 ValLeuGluMetMetHisLeuLeuLeuAspIleLeuGlnAlaProAsp 195200205 CCGTCTACATTAGAGACATTTCTGGGAAGACTTCCCATGGTGTTTAAT671 ProSerThrLeuGluThrPheLeuGlyArgLeuProMetValPheAsn 210215220 GTGGTCATTTTGTCTGTACATGGATATTTTGGACAGGCACATGTGCTC719 ValValIleLeuSerValHisGlyTyrPheGlyGlnAlaHisValLeu 225230235 GGCTTGCCTGACACTGGTGGGCAGATAGTTTATATACTTGACCAAGTG767 GlyLeuProAspThrGlyGlyGlnIleValTyrIleLeuAspGlnVal 240245250255 CGGTCTCTGGAACATGAAATGCTCCAACGAATAAAGAAGCAAGGACTA815 ArgSerLeuGluHisGluMetLeuGlnArgIleLysLysGlnGlyLeu 260265270 GATGTGACTCCTAGAATTCTTATCGTGAGTCGGTTGATTCCTGACGCT863 AspValThrProArgIleLeuIleValSerArgLeuIleProAspAla 275280285 AAAGGGACCACGTGCAATCAACGTATGGAGAAAGTCAGTGGAACAGAG911 LysGlyThrThrCysAsnGlnArgMetGluLysValSerGlyThrGlu 290295300 CATGCTAGTATCCTGAGAGTTCCTTTCCGATCAGAGAAAGGAATCCTC959 HisAlaSerIleLeuArgValProPheArgSerGluLysGlyIleLeu 305310315 CGCAAATGGATATCTAGATTTGATGTATGGCCTTATTTAGAGACCTTC1007 ArgLysTrpIleSerArgPheAspValTrpProTyrLeuGluThrPhe 320325330335 ACTGAGGATGCAGCTGGTGAAATTATTGGCGAGTTGCAGGGTCGTCCA1055 ThrGluAspAlaAlaGlyGluIleIleGlyGluLeuGlnGlyArgPro 340345350 GATCTGATAATTGGCAACTACAGCGATGGGAATATAGTTGCTTCTTTA1103 AspLeuIleIleGlyAsnTyrSerAspGlyAsnIleValAlaSerLeu 355360365 TTGTCCCACAAAATGGGTGTCACCCAGTGCAATATAGCCCATGCATTG1151 LeuSerHisLysMetGlyValThrGlnCysAsnIleAlaHisAlaLeu 370375380 GAGAAAACCAAGTATCCAGATTCTGATATTTACTGGAAAAGATTTGAG1199 GluLysThrLysTyrProAspSerAspIleTyrTrpLysArgPheGlu 385390395 GACAAATATCACTTCTCGTGTCAATTTTCAGCTGACTTGATGGCAATG1247 AspLysTyrHisPheSerCysGlnPheSerAlaAspLeuMetAlaMet 400405410415 AATCATGCTGATTTCATCATTACGAGTACTTACCAAGAGATAGCTGGA1295 AsnHisAlaAspPheIleIleThrSerThrTyrGlnGluIleAlaGly 420425430 ACGAAGAATACTGTTGGTCAATATGAAAGCCATAAGGCCTTTACTTTT1343 ThrLysAsnThrValGlyGlnTyrGluSerHisLysAlaPheThrPhe 435440445 CCGGGGCTGTATCGGGTGGTTCACGGGATTGATGTCTTTGATCCCAAG1391 ProGlyLeuTyrArgValValHisGlyIleAspValPheAspProLys 450455460 TTTAATATTGTCTCGCCAGGGGCAGACATGGCCATCTACTTCCCATTT1439 PheAsnIleValSerProGlyAlaAspMetAlaIleTyrPheProPhe 465470475 TCAGAGAAGGATGTCACCTGTCTCACTTCACTTCATAGACTTATAGAG1487 SerGluLysAspValThrCysLeuThrSerLeuHisArgLeuIleGlu 480485490495 CAGCTCCTATTCAAACCTGAGCAGAACGAAGAACACATTGGTGTATTA1535 GlnLeuLeuPheLysProGluGlnAsnGluGluHisIleGlyValLeu 500505510 GATGATACCTCAAAGCCAATTATATTTTCCATGGCGAGGCTAGACCGT1583 AspAspThrSerLysProIleIlePheSerMetAlaArgLeuAspArg 515520525 GTGAAGAATATAACAGGGCTGGTAGAGTGCTATGGCAAGAATGCGAAA1631 ValLysAsnIleThrGlyLeuValGluCysTyrGlyLysAsnAlaLys 530535540 CTCAGGGAACTGGCAAACCTGGTTGTAGTGGCTGGGTACAATGATGTA1679 LeuArgGluLeuAlaAsnLeuValValValAlaGlyTyrAsnAspVal 545550555 AAAAAGTCGAATGACAGGGAGGAAATTGCCGAAATCGAGAAGATGCAC1727 LysLysSerAsnAspArgGluGluIleAlaGluIleGluLysMetHis 560565570575 AGGCTTATACAGGAGTATAATTTAAGAGGACAATTTCGCTGGATTGCT1775 ArgLeuIleGlnGluTyrAsnLeuArgGlyGlnPheArgTrpIleAla 580585590 TCTCAAACAAATAGAGTACGAAATGGTGAACTCTATCGCTACATTTGT1823 SerGlnThrAsnArgValArgAsnGlyGluLeuTyrArgTyrIleCys 595600605 GACAAAGGAGGTATTTTTGCGCAGCCTGCATTTTATGAAGCATTTGGG1871 AspLysGlyGlyIlePheAlaGlnProAlaPheTyrGluAlaPheGly 610615620 CTTACAGTTGTTGAAGCCATGACCTGTGGTCTTCCCACATTTGCTACC1919 LeuThrValValGluAlaMetThrCysGlyLeuProThrPheAlaThr 625630635 TGCCACGGTGGTCCAGCTGAGATTATAGAAGACGGTGTTTCAGGATTT1967 CysHisGlyGlyProAlaGluIleIleGluAspGlyValSerGlyPhe 640645650655 CATATCGATCCATATCATGCTGATCAGGCAGAAAAAATGACTGAATTC2015 HisIleAspProTyrHisAlaAspGlnAlaGluLysMetThrGluPhe 660665670 TTTGTCAAGTGCAGAGAGGATCCAAACTACTGGACTAAAATCTCTGCA2063 PheValLysCysArgGluAspProAsnTyrTrpThrLysIleSerAla 675680685 GGAGGGTTACTAAGGATCAAAGAAAGATATACCTGGCAAAAGTATTCT2111 GlyGlyLeuLeuArgIleLysGluArgTyrThrTrpGlnLysTyrSer 690695700 GAAAGGTTAATGACATTGGCAGGGGTGTATGGTTTCTGGAAATATGTC2159 GluArgLeuMetThrLeuAlaGlyValTyrGlyPheTrpLysTyrVal 705710715 TCTAAACTAGAGAGAAGAGAGACACGACGTTATCTTGAGATGTTCTAC2207 SerLysLeuGluArgArgGluThrArgArgTyrLeuGluMetPheTyr 720725730735 ATTTTGAAGTTCCGTGATCTGGCCAACTCTGTTCCGCTGGCAACAGAT2255 IleLeuLysPheArgAspLeuAlaAsnSerValProLeuAlaThrAsp 740745750 GAAGAGCCTTCTACTACTGATGCAGTTGCGACATTCCGTGGACCTTGA2303 GluGluProSerThrThrAspAlaValAlaThrPheArgGlyPro* 755760765 ACGCTGCTGCTTACTGAGGTTCCAAGTTGTGTATATATTACTGTGAAAGGAATAAGTGTA2363 GCTACACAAAAGGTTCTCAACTATTAGTATCTTCTCTGTGTAAATAACGAGAGTGAAAAA2423 TGTAATATTGTTGATGTCTTGAAAACTGAGTTTGCTTTGTTTATTTTTAAGTGTATGACA2483 ATATGTATCATATAACGGATTCTTCAGTGATCATATCAAAAACTACTGACCATCGAAGTT2543 AATGAAAATCGACAGCAACA2563 (2) INFORMATION FOR SEQ ID NO:8: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 766 amino acids (B) TYPE: amino acid (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8: AlaGlyGlyLysGlnIleLeuSerAspGlyProPheSerGluValLeu 151015 ArgSerAlaGlnGluAlaIleValValProProPheValAlaIleAla 202530 ValArgProArgProGlyValTrpGluTyrValArgValAsnValSer 354045 GluLeuAsnValGluGlnLeuThrValSerGluTyrLeuHisPheLys 505560 GluGluLeuValAspGlyLysAlaAspAspHisTyrValLeuGluLeu 65707580 AspPheGluProPheAsnGluSerValProArgProThrArgSerSer 859095 SerIleGlyAsnGlyValGlnPheLeuAsnArgHisLeuSerSerSer 100105110 MetPheCysAsnLysAspCysLeuGluProLeuLeuAspPheLeuArg 115120125 ValHisLysHisLysGlyValValMetMetLeuAsnAspArgIleGln 130135140 ThrIleGlnArgLeuGlnSerAlaLeuSerLysAlaGluAspTyrLeu 145150155160 IleLysLeuProAlaAspThrProTyrSerGluPheGluPheValIle 165170175 GlnGlyMetGlyPheGluArgGlyTrpGlyAspThrAlaGluArgVal 180185190 LeuGluMetMetHisLeuLeuLeuAspIleLeuGlnAlaProAspPro 195200205 SerThrLeuGluThrPheLeuGlyArgLeuProMetValPheAsnVal 210215220 ValIleLeuSerValHisGlyTyrPheGlyGlnAlaHisValLeuGly 225230235240 LeuProAspThrGlyGlyGlnIleValTyrIleLeuAspGlnValArg 245250255 SerLeuGluHisGluMetLeuGlnArgIleLysLysGlnGlyLeuAsp 260265270 ValThrProArgIleLeuIleValSerArgLeuIleProAspAlaLys 275280285 GlyThrThrCysAsnGlnArgMetGluLysValSerGlyThrGluHis 290295300 AlaSerIleLeuArgValProPheArgSerGluLysGlyIleLeuArg 305310315320 LysTrpIleSerArgPheAspValTrpProTyrLeuGluThrPheThr 325330335 GluAspAlaAlaGlyGluIleIleGlyGluLeuGlnGlyArgProAsp 340345350 LeuIleIleGlyAsnTyrSerAspGlyAsnIleValAlaSerLeuLeu 355360365 SerHisLysMetGlyValThrGlnCysAsnIleAlaHisAlaLeuGlu 370375380 LysThrLysTyrProAspSerAspIleTyrTrpLysArgPheGluAsp 385390395400 LysTyrHisPheSerCysGlnPheSerAlaAspLeuMetAlaMetAsn 405410415 HisAlaAspPheIleIleThrSerThrTyrGlnGluIleAlaGlyThr 420425430 LysAsnThrValGlyGlnTyrGluSerHisLysAlaPheThrPhePro 435440445 GlyLeuTyrArgValValHisGlyIleAspValPheAspProLysPhe 450455460 AsnIleValSerProGlyAlaAspMetAlaIleTyrPheProPheSer 465470475480 GluLysAspValThrCysLeuThrSerLeuHisArgLeuIleGluGln 485490495 LeuLeuPheLysProGluGlnAsnGluGluHisIleGlyValLeuAsp 500505510 AspThrSerLysProIleIlePheSerMetAlaArgLeuAspArgVal 515520525 LysAsnIleThrGlyLeuValGluCysTyrGlyLysAsnAlaLysLeu 530535540 ArgGluLeuAlaAsnLeuValValValAlaGlyTyrAsnAspValLys 545550555560 LysSerAsnAspArgGluGluIleAlaGluIleGluLysMetHisArg 565570575 LeuIleGlnGluTyrAsnLeuArgGlyGlnPheArgTrpIleAlaSer 580585590 GlnThrAsnArgValArgAsnGlyGluLeuTyrArgTyrIleCysAsp 595600605 LysGlyGlyIlePheAlaGlnProAlaPheTyrGluAlaPheGlyLeu 610615620 ThrValValGluAlaMetThrCysGlyLeuProThrPheAlaThrCys 625630635640 HisGlyGlyProAlaGluIleIleGluAspGlyValSerGlyPheHis 645650655 IleAspProTyrHisAlaAspGlnAlaGluLysMetThrGluPhePhe 660665670 ValLysCysArgGluAspProAsnTyrTrpThrLysIleSerAlaGly 675680685 GlyLeuLeuArgIleLysGluArgTyrThrTrpGlnLysTyrSerGlu 690695700 ArgLeuMetThrLeuAlaGlyValTyrGlyPheTrpLysTyrValSer 705710715720 LysLeuGluArgArgGluThrArgArgTyrLeuGluMetPheTyrIle 725730735 LeuLysPheArgAspLeuAlaAsnSerValProLeuAlaThrAspGlu 740745750 GluProSerThrThrAspAlaValAlaThrPheArgGlyPro 755760765 __________________________________________________________________________

## Claims

1. An isolated DNA molecule comprising a DNA sequence, wherein the sequence comprises Seq. ID No. 1.

2. An isolated DNA molecule comprising a DNA sequence wherein the sequence comprises Seq. ID No. 3.

3. An isolated DNA molecule comprising a DNA sequence wherein the sequence comprises Seq. ID No. 5.

4. An isolated DNA molecule comprising a DNA sequence wherein the sequence comprises Seq. ID No. 7.

5. An isolated DNA molecule selected from the group consisting of: (i) SEQ ID NO. 1; (ii) SEQ ID NO. 3; (iii) SEQ ID No. 5; (iv) SEQ ID NO. 7; and (v) SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, and SEQ ID NO. 7.

6. An isolated DNA molecule having a sequence selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, and SEQ ID NO. 7.

7. An isolated DNA molecule having a nucleotide sequence comprising SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, and SEQ ID NO. 7.

8. A plasmid comprising at least one isolated DNA molecule as in any ot claims 1, 2, 3, 4, 5, 6, or 7.

9. A plasmid comprising: NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, and SEQ ID NO, 7, wherein said coding sequence, upon expression produces a protein; and

10. A plasmid comprising: NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, and SEQ ID NO. 7, wherein said coding sequence is in an anti-sense orientation with respect to the promoter; and

11. A method for producing a transformed plant cell comprising transforming the plant cell so that it comprises and expresses an isolated DNA molecule as claimed in any one of claims 1, 2, 3, 4, 5, 6, or 7.

12. A method for producing a transformed plant comprising transforming a plant cell so that it comprises and expresses an isolated DNA molecule as claimed in any one of claims 1, 2, 3, 4, 5, 6, or 7 and regenerating the transformed plant from the plant cell.

13. A method for producing a transformed plant cell comprising transforming the plant cell so that it comprises an isolated DNA molecule as claimed in any one of claims 1, 2, 3, 4, 5, 6, or 7 in an anti-sense orientation.

14. A method for producing a transformed plant comprising transforming a plant cell so that it comprises an isolated DNA molecule as claimed in any one of claims 1, 2, 3, 4, 5, 6, or 7 in an anti-sense orientation and regenerating the transformed plant from the plant cell.

15. A method for producing a transformed plant cell comprising transforming the plant cell so that it comprises the plasmid of claim 8 and expresses the isolated DNA molecule therein.

16. A method for producing a transformed plant comprising transforming a plant cell so that it comprises the plasmid of claim 8 and expresses the isolated DNA molecule therein, and regenerating the transformed plant from the plant cell.

17. A method for producing a transformed plant cell comprising transforming the plant cell so that it comprises the plasmid of claim 8 and the isolated DNA molecule therein is in an anti-sense orientation.

18. A method for producing a transformed plant comprising transforming a plant cell so that it comprises the plasmid of claim 8 and the isolated DNA molecule therein is in an anti-sense orientation, and regenerating the transformed plant from the plant cell.

19. A method of producing a plant or plant cell comprising the steps of: NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, and SEQ ID NO. 7 positioned in a sense orientation; and cell.

20. A method of producing a plant or plant cell comprising the steps of: NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, and SEQ ID NO. 7 positioned in an anti-sense orientation; and cell.

21. A transformed plant cell comprising at least one isolated DNA molecule as in any of claims 1, 2, 3, 4, 5, 6, or 7.

22. A transformed plant comprising at least one isolated DNA molecule as in any of claims 1, 2, 3, 4, 5, 6, or 7.

23. A transgenic sugar beet plant comprising at least one isolated DNA molecule as in any of claims 1, 2, 3, 4, 5, 6, or 7.

24. A plant or plant cell transformed with a plasmid according to claim 9.

25. A plant or plant cell transformed with a plasmid according to claim 10.

26. A transformed plant cell comprising the plasmid of claim 8.

27. A transformed plant comprising the plant cell of claim 21.

28. A transformed plant comprising the plant cell of claim 26.

29. A transformed plant comprising the plasmid of claim 8.

30. A transformed plant comprising an isolated DNA molecule having a nucleotide sequence comprising SEQ ID. NO. 1.

31. A transformed plant comprising an isolated DNA molecule having a nucleotide sequence comprising SEQ ID. NO. 3.

32. A transformed plant comprising an isolated DNA molecule having a nucleotide sequence comprising SEQ ID. NO. 5.

33. A transformed plant comprising an isolated DNA molecule having a nucleotide sequence comprising SEQ ID. NO. 7.

34. A transformed plant comprising an isolated DNA molecule having a nucleotide sequence comprising SEQ ID. NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, and SEO ID NO. 7.

35. A transformed plant cell comprising an isolated DNA molecule therein, and regenerating the plant from the plant cell having a nucleotide sequence selected from the group consisting of: SEQ ID. NO. 1; SEQ ID NO. 3; SEQ ID NO. 5; SEQ ID NO. 7; and SEQ ID. NO. 1, SEQ ID NO. 3, SEQ ID NO. 5, and SEQ ID NO. 7.