NISTIR 7318 

Description and Results of the 2001 NIST/NOAA Interlaboratory 

Comparison Exercise Program for Organic Contaminants in 

Marine Mammal Tissues 

John R. Kucklick 

Rebecca S. Pugh 

Paul R. Becker 

Michele M. Schantz 

Stephen A. Wise 

Teresa K. Rowles 

 
NISTIR 7318 

Description and Results of the 2001 NIST/NOAA Interlaboratory 

Comparison Exercise Program for Organic Contaminants in 

Marine Mammal Tissues 

John R. Kucklick 

Rebecca S. Pugh 

Paul R. Becker 

Chemical Science and Technology Laboratory 

Analytical Chemistry Division 

Charleston Laboratory 

Michele M. Schantz 

Stephen A. Wise 

Chemical Science and Technology Laboratory 

Analytical Chemistry Division 

Teresa K. Rowles 

National Oceanic and Atmospheric Administration 

National Marine Fisheries Service 

September 2005

U.S. Department of Commerce 

Carlos M. Gutierrez, Secretary 

National Institute of Standards and Technology 

William A. Jeffrey, Director 
ii

TABLE OF CONTENTS 

LIST OF APPENDICES ............................................................................................................III

LIST OF TABLES ......................................................................................................................IV

ABSTRACT.................................................................................................................................VI

INTRODUCTION......................................................................................................................... 1

Background on Interlaboratory Comparison Exercises and Associated Quality 

Assurance Activities Conducted by NIST for the MMHSRP................................................ 2

Preparation, Analysis, and Distribution of Control Materials ................................................ 2 

Development of Standard Reference Materials (SRMs) .......................................................... 3 

Interlaboratory Comparison Exercises.................................................................................... 4 

MATERIALS USED IN THE 2001 EXERCISE ....................................................................... 8

Exercise Requirements and Target Analytes.......................................................................... 8

EVALUATION OF THE EXERCISE RESULTS................................................................... 11

Determination of Laboratory Analyte Means....................................................................... 11

Establishment of Consensus Values....................................................................................... 11

Reported Results...................................................................................................................... 11

Assignment of z-and p-scores ................................................................................................. 11

RESULTS AND DISCUSSION ................................................................................................. 13

CONCLUSIONS ......................................................................................................................... 14

LITERATURE CITED .............................................................................................................. 15
iii

LIST OF APPENDICES 

A. Tabular results of PCB congener, pesticide, and lipid data reported by all 

laboratories ..........................................................................................................................A-1-37 

B. Graphical results of PCB congener and lipid data reported by all laboratories. The 

Z-scores for Control Material V represent 25% of the assigned value so that Z = +1 

is the assigned value plus 25 %, Z = -1 is the assigned value minus 25 % and so 

forth. Error bars are ± 1 standard deviation. ..................................................................B-1-31 

C. Graphical results of pesticide and lipid data reported by all laboratories. The Zscores for Control Material V represent 25% of the assigned value so that Z = +1 is 

the assigned value plus 25 %, Z = -1 is the assigned value minus 25 % and so forth. 

Error bars are ± 1 standard deviation.................................................................................C-1-20 

D. Tabular summary of methods used for analysis by each laboratory..............................D-1-8 

E. Additional analyte data reported by individual laboratories ..........................................E-1-4 
iv

LIST OF TABLES 

Table 1: Interlaboratory Comparison Exercises Conducted for the 

MMHSRP from 1991-2001..............................................................................................................6 

Table 2: Laboratories Participating in the NIST/NOAA Interlaboratory 

Comparison Exercise Program for Organic Contaminants .......................................................9 

Table 3: Target Analytes for the NIST/NOAA Interlaboratory Comparison 

Exercise Program for Organic Contaminants in Marine Mammal Tissues ..............................10 

Table 4: PCB Congeners in Control Material V (ng/g wet mass) Reported by 

the Participants the NIST/NOAA Interlaboratory Comparison Exercise 

Program for Organic Contaminants in Marine Mammal Tissues..............................................18 

Table 5: Organochlorine Pesticides in Control Material V (ng/g wet mass) 

Reported by the Participants in the NIST/NOAA Interlaboratory 

Comparison Exercise Program for Organic Contaminants in Marine 

Mammal Tissues...............................................................................................................................19 

Table 6: PCB Congeners in SRM 1945 (ng/g wet mass) Reported by the 

Participants in the NIST/NOAA Interlaboratory Comparison Exercise 

Program for Organic Contaminants in Marine Mammal Tissues..............................................20 

Table 7: Organochlorine Pesticides in SRM 1945 (ng/g wet mass) Reported 

by the Participants in the NIST/NOAA Interlaboratory Comparison Exercise 

Program for Organic Contaminants in Marine Mammal Tissues..............................................21 
v

DISCLAIMER 

Certain commercial equipment or instruments are identified in this report to specify adequately 

the experimental procedures. Such identification does not imply recommendations or 

endorsement by the National Institute of Standards and Technology nor does it imply that the 

equipment or instruments are the best available for the purpose. 
vi

ABSTRACT 

The National Institute of Standards and Technology (NIST), in support of the National Oceanic 

and Atmospheric Administration’s Marine Mammal Health and Stranding Response Program 

(NOAA/MMHSRP) conducts annual interlaboratory comparison exercises for the determination 

of chlorinated pesticides, polychlorinated biphenyl congeners, and trace elements in marine 

mammal tissues. These exercises provide one mechanism for laboratories to evaluate their 

measurement quality and comparability for these constituents in marine mammal tissues. 

Eighteen laboratories participated in determining the concentrations of selected polychlorinated 

biphenyl congeners (PCBs) and organochlorine pesticides in a homogenized blubber control 

material “Marine Mammal Quality Assurance Exercise Control Material V” (Control Material 

V) and Standard Reference Material (SRM) 1945 Organics in Whale Blubber. This report 

includes the results reported by the participating laboratories, combined consensus data results, 

and summary statistics for each analyte in the samples. The numerical indices used to assess 

laboratory performance are also discussed. 
1

INTRODUCTION 

Laboratories measuring contaminants in the marine environment must assess the accuracy and 

precision of their measurements. Quality control of measurements made on marine 

environmental samples is vital to the accurate assessment of marine pollution and its effects on 

wildlife and human health. Often, reference materials are limited or not available for many 

marine matrices of interest (e.g., marine mammal tissues and marine fish). Consequently, marine 

resource management decisions may be based on subjective analytical results leading to potential 

environmental, health, or economic consequences. The National Institute of Standards and 

Technology’s (NIST’s) Analytical Chemistry Division has several programs to assess the data 

quality of laboratories and agencies performing chemical measurements on marine-related 

samples. NIST’s reference material production, interlaboratory comparison exercises, and 

environmental specimen banking all contribute to the accuracy of chemical measurements made 

on samples from the marine environment. 

NIST helps benchmark and improve the quality of analytical data gathered on the marine 

environment by administering annual interlaboratory comparison exercises. The largest exercise 

was initiated in 1987 and funded in part until 2000 by the National Oceanic and Atmospheric 

Administration (NOAA) National Status and Trends Marine Monitoring Program 

(NOAA/NS&T) (Cantillo and Parris, 1990, 1993; Cantillo 1995; Schantz et al., 1999). NIST 

provides mechanisms for assessing the interlaboratory and temporal comparability of data and 

for improving measurements of polycyclic aromatic hydrocarbons, polychlorinated biphenyl 

(PCB) congeners, and chlorinated pesticides in bivalve mollusk, sediment, and fish samples. In 

addition, the National Research Council of Canada, with support from NOAA, administers a 

similar interlaboratory comparison exercise for trace elements in marine environmental

materials. The NIST program for organic contaminants includes developing improved analytical 

methods, producing NIST Standard Reference Materials (SRMs) and other control materials, 

conducting annual interlaboratory comparison exercises, and coordinating workshops to discuss 

exercise results, thus providing a cooperative problem-solving forum for the participants. This 

program continues as the NIST Intercomparison Program for Organic Contaminants in the 

Marine Environment with partial support from fees paid by the participants (e.g., Schantz et al. 

2002).. 

Through the NIST National Marine Analytical Quality Assurance Program and with support 

from the NOAA Marine Mammal Health and Stranding Response Program (MMHSRP), the 

interlaboratory comparison activities have been expanded to include analyses of marine mammal 

tissues. The 2001 NIST/NOAA Interlaboratory Comparison Exercise Program for Organic 

Contaminants in Marine Mammal Tissues was modeled after the exercises described above. 

Specifically, this exercise was designed to help laboratories assess data comparability and quality 

relative to other groups providing measurements of organochlorine contaminants and trace 

elements in marine mammal tissues and to link these important measurements to a national 

metrology laboratory. The results of the exercises presented in this report should be useful for 

both assessing current methodology and reducing the variability of contaminant data reported on 

marine mammals. Future exercises will allow for the assessment of analytical data quality over 

time. This report summarizes the results of the 2001 organic contaminant exercise including 

methods used for analysis, data reported by the laboratories on the intercomparison materials, 
2

and numerical indices used to assess laboratory performance. A report synthesizing the 2001 

trace element results of this exercise is in preparation and results from the 2000 exercise are 

available in Kucklick et al., 2002. 

Background on Interlaboratory Comparison Exercises and Associated Quality Assurance 

Activities Conducted by NIST for the MMHSRP 

In 1987, the NOAA National Marine Fisheries Service (NMFS), Office of Protected Resources, 

established the National Marine Mammal Tissue Bank (NMMTB) as part of the National 

Biomonitoring Specimen Bank maintained by NIST. The NMMTB was designed for the longterm cryogenic archiving of marine mammal tissue specimens for future retrospective chemical 

analysis. In 1992, the Marine Mammal Health and Stranding Response Act (Public Law 102-

587) formally established the NMMTB. NMFS implemented this Act by instituting the 

MMHSRP. Components of this program include marine mammal stranding networks, response 

to unusual mortality events, information management, real-time measurement of tissue 

contaminants, specimen banking, and analytical quality assurance. 

Within the MMHSRP, real-time monitoring of contaminants in marine mammals is conducted by 

NMFS’s Northwest Fisheries Science Center (NWFSC), Environmental Conservation Division. 

This monitoring includes analyses of samples collected specifically for real-time analysis and 

subsamples of banked specimens. NIST also analyzes aliquots of banked specimens to establish 

a baseline of concentration values for comparing with data generated by the NWFSC and other 

laboratories analyzing these specimens, and to monitor changes in analyte levels during 

specimen storage. Numerous other laboratories worldwide analyze marine mammal tissues for 

research and monitoring purposes. To assess the accuracy and comparability of results among 

NIST, NWFSC, and other laboratories, NIST in collaboration with NMFS developed a quality 

assurance (QA) program for analytical measurements of contaminants in marine mammal 

tissues. This QA program, described by Wise et al. (1993), consists of (1) preparation, analysis, 

and distribution of marine mammal tissue control materials; (2) development of Standard 

Reference Materials (SRMs) for use in the analysis of marine mammal tissues; and (3) 

interlaboratory comparison exercises among laboratories analyzing marine mammal tissues. 

Preparation, Analysis, and Distribution of Control Materials 

 

Control materials, which are similar to the matrices being analyzed, are large batches of 

homogeneous material of which an aliquot can be used routinely in the analysis of unknown 

samples. They are useful for assessing the reproducibility of analyses. Given enough data, the 

materials may be value-assigned for a given constituent. The first control materials developed 

for the program were derived from liver and blubber tissues collected from pilot whales 

(Globicephala melaena) stranded in 1990 on Cape Cod, MA. Approximately 2 kg of each tissue 

were used to prepare tissue homogenates for use as analytical control materials and in 

interlaboratory comparison exercises. These homogenates are fresh frozen samples similar to 

marine mammal tissue samples routinely analyzed, rather than freeze-dried matrices frequently 

used as reference materials. The tissues were cryogenically pulverized and homogenized in 

Teflon mills to provide frozen powder-like materials (Zeisler et al., 1983). These two control 

materials were analyzed at NIST to determine concentrations of trace elements (Whale Liver 
3

Homogenate I) and organic contaminants (Whale Blubber Control Material) and to assess 

sample homogeneity (Wise et al., 1993). 

The concentrations of 40 trace elements plus methylmercury were determined in the Whale Liver 

Homogenate I using INAA, differential pulse and square wave stripping voltammetry, cold vapor 

atomic absorption spectroscopy (CVAAS) for Hg, and ion-exchange chromatography plus 

CVAAS (for methylmercury). Thirty PCB congeners and 16 chlorinated pesticides were 

determined in the Whale Blubber Control Material using gas chromatography-electron capture 

detection (GC-ECD) with both DB-5 and C-18 columns and gas chromatography-mass 

spectrometry (GC/MS) using a DB-5 column. The data resulting from analyses of both control 

materials, as well as a description of the analytical techniques and interpretation of the results, 

were published in Wise et al. (1993). 

In the 1999 interlaboratory comparison exercise, Whale Blubber Control Material was re-labeled 

Marine Mammal Blubber Control Material III, and distributed as an “unknown” to participants. 

Because the amount of this control material is now very limited, Whale Blubber Control 

Material IV is presently being developed as a replacement. Whale Blubber Control Material IV

was derived from blubber that was collected by the NMFS Beaufort Laboratory, from a single 

pilot whale that stranded in 1999 on Pea Island, North Carolina. This material was cryogenically 

homogenized and labeled, QC00-WB4 Whale Blubber Control Material IV, and was distributed 

as an “unknown” to participants in the 2000 interlaboratory comparison exercise for the organic 

analysis (Kucklick et al., 2002) 

Development of Standard Reference Materials (SRMs) 

One of the goals of the marine mammal QA program is to develop certified reference materials 

(CRMs) for validating analytical measurements of trace elements and organic contaminants in 

marine mammal tissues. At the beginning of the program, there were several CRMs available 

from NIST, the National Research Council of Canada (Ottawa, Canada), and the Community 

Bureau of Reference (Brussels, Belgium) for inorganic contaminants in marine tissues including 

oyster tissue, mussel tissue, fish muscle and liver tissue, and lobster tomalley. However, for 

organic contaminants such as PCB congeners and chlorinated pesticides, there were only mussel 

tissue, SRM 1974 Organics in Mussel Tissue (Mytilus edulis) and cod liver oil, SRM 1588 

Organics in Cod Liver Oil. SRM 1588, which serves as a suitable surrogate for a tissue extract 

with a high lipid content, had certified concentrations for 5 PCB congeners and 10 chlorinated 

pesticides and noncertified values for 20 additional PCB congeners and 4 additional chlorinated 

pesticides (Schantz et al., 1992). This material was reissued in 1998 as SRM 1588a and now 

has certified or reference values for over 80 organic contaminants. The mussel tissue SRM 1974 

was reissued as SRM 1974a and has certified or reference values for over 100 organic and 

inorganic constituents (Schantz et al., 1997). Due to the popularity of SRM 1974a, a new mussel 

tissue SRM, SRM 1974b, has recently been issued (Poster et al., 2003) 

Because of the lack of organic reference materials, blubber was selected as the first priority 

tissue for development of SRMs as part of the marine mammal QA Program. The experience 

gained from the preparation and analysis of the pilot whale blubber control material (Wise et al.,
4

1993) was used to develop SRM 1945 Organics in Whale Blubber, a certified material that can 

be used for validating measurements of organic contaminants in marine mammal blubber. 

SRM 1945 was prepared from approximately 15 kg of blubber that was collected in September 

1991 from a stranding of pilot whales on Cape Cod, MA. The material was cryogenically 

pulverized and homogenized in the same manner as described for the control materials. The 

resulting frozen blubber homogenate was analyzed using three different analytical techniques; 

two techniques were based on GC-ECD using two capillary columns of differing in selectivity 

and one method that utilized gas chromatography-mass spectrometry (GC/MS). The results of 

these three techniques provided certified concentrations for 27 PCB congeners (PCBs 18, 44, 49, 

52, 66, 87, 95, 99, 101/90, 105, 110, 118, 128, 138/163/164, 149, 151, 153, 156, 170/190, 180, 

183, 187, 194, 195, 201, 206, and 209) and 15 chlorinated pesticides (HCB, α-HCH, γ-HCH, 

heptachlor epoxide, oxychlordane, mirex, cis-chlordane, cis-nonachlor, trans-nonachlor, 2,4'-

DDE, 4,4'-DDE, 2,4'-DDD, 4,4'-DDD, 2,4’-DDT, and 4,4'-DDT). Noncertified values for two 

additional PCB congeners (PCB 28 and 31) and chlorinated pesticides (dieldrin and β-HCH) are 

available.

Analytical data for the certification of PCBs and chlorinated pesticides in SRM 1945 were 

published in Schantz et al. (1995). SRM 1945 complements the other frozen tissue SRM (e.g.,

SRM 1974b) by providing concentrations that are generally a factor of 10 to 100 times higher for 

the PCB congeners and chlorinated pesticides. Solvent extraction of the whale blubber produces 

an oil matrix similar to that of the cod liver oil SRM; however, the concentrations of PCB 

congeners and pesticides in SRM 1945 are generally 2 to 3 times lower than the NIST cod liver 

oil SRM except for the PCB congeners with higher degrees of chlorination, which have 

concentrations similar to the cod liver oil. 

Additional concentration values for constituents not originally measured in SRM 1945 (i.e.,

dioxins, furans, non-ortho PCBs, and polybrominated diphenyl ethers) have been provided for 

this material by the National Wildlife Research Center, Canadian Wildlife Service (CWS). 

These data are found in CWS Lab Services Section Reports CHEM-OC-97-40, CHEM-PCDD98-1, and CHEM-PCDD-98-4). SRM 1945 was found to have relatively high amounts of twelve 

polybrominated diphenyl ethers (tetra through hepta congeners). Data are also available on 

chiral organochlorine pollutants in SRM 1945 and SRM 1588 (Wong et al., 2002). More 

recently, measurements have been made by NIST on additional analytes in SRM 1945, including 

congeners of polychlorinated naphthalenes (PCNs), co-planar PCBs (77, 126, 169), 

polybrominated diphenyl ether congeners, and toxaphene (Kucklick et al., 2003). 

The low relative uncertainties associated with the majority of analyte concentrations (5 % to 10 

%) and the extensive list of certified analytes makes SRM 1945 a valuable resource for 

validating analytical methods for the determination of halogenated compounds in marine 

mammal blubber and other high lipid-containing materials.

 

Interlaboratory Comparison Exercises 

An interlaboratory comparison made on common, well-homogenized reference samples is one 

method to assess and improve data comparability. Such exercises currently involve the analysis 
5

of an unknown sample and a NIST-traceable SRM by the participants. A list of analytes to be 

compared among the laboratories is provided to the participants. Participants submit their results 

to the NIST coordinator who evaluates data comparability using the performance measures 

recommended by the International Union of Pure and Applied Chemistry (IUPAC, 1993). 

Reports on the comparability evaluation are provided to the participants. NIST then conducts a 

workshop with the participants to discuss the results and ways to improve comparability. 

Table 1 summarizes several important aspects of the interlaboratory comparison exercises 

conducted from 1991 to date, including target analytes, matrix samples, and the number of 

participating laboratories. The first exercise was conducted in 1991-92 and consisted of the 

distribution of the liver and blubber control materials to NWFSC and several other laboratories 

for analysis, i.e., Department of Fisheries and Oceans (DFO) Canada in Winnipeg, the 

Geochemical and Environmental Research Group (GERG) at Texas A&M University, and the 

Department of Analytical and Environmental Chemistry at the University of Ulm in Germany. 

In 1992, three laboratories (NIST, DFO Canada in Winnipeg, and the Department of Analytical 

and Environmental Chemistry at the University of Ulm in Germany) analyzed blubber 

subsamples from four to six beluga whales, the pilot whale blubber control material, and SRM 

1588 in an interlaboratory exercise to compare results of analyses for PCB congeners and 

chlorinated pesticides. The analytical methods used by these three laboratories are described in 

Schantz et al. (1996). To minimize variability resulting from the source of calibration solutions, 

all three laboratories used common solutions to prepare calibration standards. Different internal 

standards and volume correction standards were used by each laboratory. Each laboratory used 

its preferred methods of extraction (Soxhlet extraction at NIST, ball-milling extraction at DFO, 

and column extraction at Ulm). All three laboratories used a size-exclusion chromatography 

cleanup, but with different columns, to remove the lipid material from the extracts and separated 

the PCB congeners from the majority of the pesticides as part of the cleanup procedures. GCECD was used by all three laboratories for the final analysis employing different columns. The 

results of this exercise were published in Schantz et al. (1996). 

In 1993, two intercomparison exercises were initiated. The first exercise focused on determining 

PCB congeners and chlorinated pesticides in blubber tissue using the blubber homogenate 

prepared as a proposed SRM (see discussion above). This material was distributed to NWFSC 

and several other laboratories for analysis (i.e., DFO Canada, GERG, Northwestern Aquatic 

Sciences in Newport, OR, Arthur D. Little in Cambridge, MA, and University of Ulm). The 

second QA activity involved a "sample split" between NIST and NWFSC of blubber and liver 

samples from three marine mammals for both inorganic and organic analyses. These tissue 

samples were from specimens collected as part of the tissue banking and monitoring components 

of the program. In the past, for many of the liver and blubber specimens in the NMMTB, similar 

tissue samples were collected using NWFSC protocols and shipped to NWFSC for analysis as 

part of the monitoring effort. For this intercomparison exercise, NIST homogenized the selected 

liver and blubber samples from the tissue bank and provided a subsample of each homogenate to 

NWFSC. NIST and NWFSC analyzed tissue homogenate subsamples from the bank (liver for 

trace elements and blubber for PCBs and pesticides). The NWFSC analyzed the "monitoring" 

liver and blubber samples from the same animals. This provided an assessment of 

interlaboratory comparability on the same tissue homogenates and the comparability of the 
6

analyses of banked and the monitoring specimens collected from the same animals. This QA 

activity was an informal exercise only; the results have not been published.

Table 1: Interlaboratory comparison exercises conducted for the MMHSRP from 1991-2001. 

Dates Analytes Matrices Objective Number of Labs 

1991-92 PCBs/Cl 

pesticides1 

Trace 

Elements 

Whale blubber 

Whale liver CM2

Laboratory comparability 

Analytical control 

5 

2 

1992-93 PCBs/Cl 

pesticides 

Whale blubber; whale 

blubber CM 

Laboratory comparability 3 

1993-1994 PCBs Whale blubber; whale 

blubber CM 

SRM development 7 

1993-1997 PCBs/Cl 

pesticides 

and Trace 

Elements 

Whale / seal liver Sample comparability 2 

1997-1998 Trace 

Elements 

Whale liver Laboratory comparability 

and CM development 

3 

1999 PCBs/Cl 

Pesticides 

SRM 1945 Organics in 

Whale Blubber 

Whale blubber CM 

Laboratory comparability 10 

2000 PCBs/Cl 

Pesticides 

Trace 

Elements 

SRM 1945 Organics in 

Whale Blubber 

Whale Livers (QC91LH1) 

(QC97LH2) 

SRM 1946 Lake Superior 

Fish Tissue 

Laboratory comparability 

and program expansion 

13 

7 

2001 PCBs/Cl 

Pesticides 

Trace 

Elements 

SRM 1945 and Whale 

blubber CM 

Whale Livers 

(QC91LH1) 

(QC97LH2) 

Candidate 

SRM 1947 Lake 

Michigan Fish Tissue 

Laboratory comparability 

and program expansion 

18 

31 

1

Chlorinated pesticides 

2

Control material 

3

Department of Fisheries and Oceans
7

In 1995 NIST, in collaboration with NOAA, formally instituted the National Marine Analytical 

Quality Assurance Program (NMAQAP). The mission of this program is to expand QA and 

specimen banking activities in marine environmental research and monitoring. To help 

accomplish the NMAQAP mission, a NIST satellite laboratory was established in Charleston, 

SC, in association with the NOAA Center for Coastal Environmental Health and Biomolecular 

Research (CCEHBR). The NMMTB is housed both at the NIST-Gaithersburg and at the NISTCharleston facilities. NIST-Charleston provides the primary infrastructure for the marine 

mammal QA program and the NMMTB. 

In 1997, an interlaboratory comparison exercise on trace elements in beluga whale liver sample 

splits was initiated among NIST-Gaithersburg, NWFSC, and the Texas A&M University 

Veterinary Medical Center. All three laboratories were involved in analyzing marine mammal 

tissues collected in Arctic Alaska. For this exercise, sample splits of beluga whale liver tissues 

collected during the 1996 subsistence hunts in Alaska were provided to the participants, as well 

as the pilot whale liver control material (Whale Liver Homogenate I) and the new beluga whale 

liver control material (Whale Liver Homogenate II). 

The 1999 exercise included only laboratories conducting organic analyses. The 10 participating 

laboratories (NIST-Gaithersburg, NIST-Charleston, CCEHBR, NWFSC, GERG, Mississippi 

State Chemistry Laboratory, University of Connecticut, National Lab for Environmental Testing 

[Canada], Ehime University [Japan], and University of Utah) measured PCB congeners and 

organochlorine pesticides in SRM 1945 and in the “unknown,” Marine Mammal Blubber

Control Material III (this material is described above in the control material section). Also in 

1999, the program began holding meetings of participants in conjunction with the annual 

meeting of the Society of Environmental Toxicology and Chemistry (SETAC). 

Participation in the 2000 exercise included laboratories conducting both organic analyses (13 

laboratories) and inorganic analyses (7 laboratories). In 2001, there were approximately 18 

laboratories participating in the organic exercise and 31 laboratories participating in the 

inorganic exercise. Expanding the number of laboratories provides an essential benefit for the 

participants in yielding higher quality consensus data. NIST also benefits from the preliminary 

concentration data and information gained on the candidate SRM materials that are routinely 

inserted into the exercises. 

The QA program performs a major function in maintaining the quality of data resulting from the 

analysis of NMMTB specimens. Scientists requesting specimens from the bank for retrospective 

studies must demonstrate their analytical capabilities through appropriate QA activities, 

including participation in the NIST-administered QA program. In addition, NMFS requires that 

all researchers analyzing marine mammal tissues for contaminants under NMFS funding be 

participants in this program. This requirement ensures that the analytical results from marine 

mammal monitoring and research programs are of high quality and comparable. 
8

MATERIALS USED IN THE 2001 EXERCISE 

The 2001 NIST/NOAA Interlaboratory Comparison Exercise for Organochlorines in Marine 

Mammal Tissues (2000 MMQA) used two materials sent out to 21 laboratories of which, 18 of 

which submitted data for this exercise (Table 2). Participants were asked to make three 

measurements each on two materials: SRM 1945 “Organics in Whale Blubber” and MMQA-V 

the “unknown.” The unknown material consisted of blubber collected from a 55.5 kg female 

California sea lion (Zalophus californianus) that stranded near San Luis Obispo, California on 

July 4, 2000. The blubber was collected by personnel from the Marine Mammal Center, 

Sausalito, California, and sent frozen to the NIST-Charleston laboratory for cryogenic storage 

and processing. At NIST-Charleston, the blubber tissue was stored in a liquid nitrogen (LN2) 

vapor phase freezer at –150 °C until prepared for this exercise. The material was trimmed on 

Teflon sheeting using a titanium knife. The material was cryohomogenized at NIST Charleston 

according to established procedure (Zeisler et al., 1983). After homogenization, the material was 

placed inside a Teflon bag and blended manually by repeated inversion. The blended material 

was bottled as 12 g subsamples in glass jars and stored at -80 °C. One bottle of this material 

along with one bottle of SRM 1945 were sent either on dry ice or using a liquid nitrogen cooled 

biological dry shipper via overnight express to each participating laboratory. 

Exercise Requirements and Target Analytes 

A suite of analytes was chosen for the exercise based on those used in the NIST/NOAA/NS&T 

exercise and several additional analytes were included to broaden this list (Table 3). 

Laboratories were requested to make triplicate measurements of these compounds in each of the 

materials and report their data using a data template provided by NIST. Laboratories were also 

asked to provide results from additional analytes, such as coplanar PCBs or chlorobenzenes that 

were determined in the two materials. Results from the exercise were discussed during a 

workshop held in conjunction with the 2001 Society of Environmental Toxicology and 

Chemistry annual meeting held in Baltimore, MD. 
9

Table 2: Laboratories Participating in the 2001 NIST/NOAA Interlaboratory Comparison 

Exercise Program for Organic Contaminants. 

Mississippi State Chemical Lab 

112 Hand Chemical Lab 

Morrill Rd. 

MS State, MS 39762 

USA 

Geochemical and Environmental Research Group 

Texas A&M University 

833 Graham Road 

TAMU Mail Stop 3149 

USA 

213 National Food Safety and Toxicology Ctr 

Michigan State University 

East Lansing, MI 48824 

USA 

Fisheries and Oceans Canada 

Institute of Ocean Sciences 

9860 West Saanich Road 

Sidney, B.C. V8L-4B2 

Environmental Research Institute 

University of Connecticut 

Storrs, CT 06269 

USA 

Kumamoto University 

2-39-1, Kurokami, Kumamoto, 860-8555 

Japan

National Laboratory for Environmental Testing 

National Water Research Institute 

Environment Canada 

867 Lakeshore Road, P.O. Box 5050 

BURLINGTON, Ontario L7R 4A6 

Canada 

Department of Environmental Sciences 

Institute of Environmental & Natural Sciences 

Lancaster University 

Bailrigg 

Lancaster LA1 4YQ 

UK 

CEFAS Burnham Laboratory 

Remembrance Avenue 

Burnham on Crouch 

Essex CM0 8HA 

UK 

Center for Marine Environmental studies, 

Ehime University. 

Tarumi 3-5-7, Matsuyama, Ehime 790-8566 

 Japan 

Baltic Sea Research Institute 

Seestrasse 15 

18119 Rostock 

Germany 

National Environmental Research Institute 

Department of Environmental Chemistry 

Frederiksborgvej 399 

DK-4000 Roskilde 

Denmark 

NOAA/National Marine Fisheries Service 

74 Magruder Rd 

Highlands, NJ 07732-0428 

USA 

NOAA/National Marine Fisheries Service 

Northwest Fisheries Science Center 

2725 Montlake Blvd East 

 Seattle, WA 98112-2097 

USA 

Energy and Geoscience Institute 

Department of Civil and Environmental Engineering 

University of Utah 

423 Wakara Way, Suite 300 

Salt Lake City Utah 84108 

USA 

NIST 

Hollings Marine Laboratory 

331 Fort Johnson Road 

Charleston, SC 29412 

USA 

NOAA/National Ocean Service 

Center for Coastal Environmental Health and 

Biomolecular Research 

219 Fort Johnson Road 

Charleston, SC 29412 

USA 

NIST 

100 Bureau Drive 

Building 224 

Gaithersburg, MD 20899-8692 

USA
10

Table 3: Target Analytes for the NIST/NOAA Interlaboratory Comparison Exercise Program 

for Organic Contaminants in Marine Mammal Tissues. 

Pesticides PCB Congeners 

Number Congener Substitution 

2,4’-DDT 18 2,2’,5-trichlorobiphenyl 

4,4’-DDT 28 2,4,4’-trichlorobiphenyl 

2,4’-DDE 31 2,4’,5-trichlorbiphenyl 

4,4’-DDE 44 2,2’,3,5’-tetrachlorobiphenyl 

2,4’-DDD 49 2,2’,4,5’-tetrachlorobiphenyl 

4,4’-DDD 52 2,2’,5,5’-tetrachlorobiphenyl 

HCB 66 2,3’,4,4’-tetrachlorobiphenyl 

α-HCH 87 2,2’,3,4,5’-tetrachlorobiphenyl 

γ-HCH 95 2,2’,3,5’,6-tetrachlorobiphenyl 

β-HCH 99 2,2’,4,4’,5-tetrachlorobiphenyl 

heptachlor epoxide 101 2,2’,4,5,5’-tetrachlorobiphenyl 

cis-chlordane 105 2,3,3’,4,4’-tetrachlorobiphenyl 

trans-chlordane 118 2,3’,4,4’,5-tetrachlorobiphenyl 

oxychlordane 128 2,2’,3,3’,4,4’-hexachlorbiphenyl 

cis-nonachlor 132 2,2’,3,3’,4,6’-hexachlorbiphenyl 

trans-nonachlor 138 2,2’,3,4,4’,5’-hexachlorbiphenyl 

dieldrin 149 2,2’,3,4’,5’,6-hexachlorobiphenyl 

mirex 151 2,2’,3,5,5’,6-hexachlorbiphenyl 

 153 2,2’,4,4’,5,5’-hexachlorbiphenyl 

 156 2,3,3’,4,4’,5-hexachlorbiphenyl 

 170 2,2’,3,3’,4,4’,5-heptachlorobiphenyl 

 180 2,2’,3,4,4’,5,5’-heptachlorobiphenyl 

 183 2,2’,3,4,4’,5’,6-heptachlorobiphenyl 

 187 2,2’,3,4’,5,5’,6-heptachlorobiphenyl 

 194 2,2’,3,3’,4,4’,5,5’-octachlorobiphenyl 

 195 2,2’,3,3’,4,4’,5,6-octachlorobiphenyl 

 201 2,2’,3,3’,4,5,5’,6’-octachlorobiphenyl 

 206 2,2’,3,3’,4,4’,5,5’,6-nonachlorobiphenyl 

 209 2,2’,3,3’,4,4’,5,5’,6,6’-decachlorobiphenyl 
11

EVALUATION OF THE EXERCISE RESULTS 

Determination of Laboratory Analyte Means 

Each laboratory reported the results of their analyses (Sample 1, Sample 2, and Sample 3) and 

the mean for each laboratory was calculated. Non-numerical results were reported as “NA” (not 

analyzed). None of the target analytes were below the limit of detection for the participating 

laboratories (Tables 4 - 7 and Appendix A). 

Establishment of Consensus Values 

The following guidelines were used by NIST for establishing the “assigned values” or 

“consensus values” for the exercise. The consensus values for Control Material V were the mean 

of all the reported laboratory means for a compound after the data were first screened by a 

Grubb’s Single Iteration Outlier Test (Motulsky, 1997). Generally, most or all the values were 

included in the determination of the consensus value. The target values for the SRM were the 

certified values, reference values, or the consensus values from the 1999 and 2000 exercises 

where SRM 1945 was also analyzed (Tables 6 and 7). In the NIST/NOAA/NS&T exercise, the 

assigned values for the unknown material were calculated based on the performance of each 

laboratory on the SRM. If the laboratory was within ± 30% of the SRM value, their values for 

the corresponding unknown were used to determine the consensus value. This approach was not 

used in the present study, since, as demonstrated in past exercises (Kucklick et al., 2002), that 

this can lead to the rejection of up to half of the data. The concentration of Control Material V 

was also significantly greater than SRM 1945 for many compounds. For instance, the 

concentration of 4,4’-DDE was 75 times greater in Control Material V than in SRM 1945. 

Hence there was concern regarding the performance on the SRM being reflective of the 

performance on the control material. 

 

Reported Results 

Laboratories were assigned a numerical identification code based on the order in which data 

were received with the exception of NIST-Charleston, which was Laboratory 1 and NIST 

Gaithersburg, which was Laboratory 17. The same code was used for both materials. The 

results from the analysis of Control Material V and SRM 1945 are summarized in Tables 4 - 7. 

Appendix A shows the tabulated results from the individual laboratories for both materials and 

the results are shown graphically in Appendix B. Appendix C gives the methods used for 

analysis by each laboratory and Appendix D shows data for additional analytes. 

Assignment of z-and p-scores 

Performance Scores: Different programs have different data quality needs. The acceptability of 

the results submitted by a laboratory will be decided by the individual program(s) for which the 

laboratory provides data. Typically, the program will use these exercise results in conjunction 

with the laboratory’s performance in the analysis of certified reference materials and/or control 

materials, and of other quality assurance samples. These exercise results are shown in a number 

of ways in this report to aid in the evaluation of data quality. 
12

IUPAC guidelines (IUPAC 1997) describe the use of “z-scores” and “p-scores” for assessment 

of accuracy and precision in interlaboratory comparison exercises, such as described in this 

report. These indices assess the difference between the result of the laboratory and the exercise 

assigned value, and can be used, with caution, to compare performance on different analytes and 

on different materials. 

Bias Assessment (z-score):

z = bias estimate / performance criterion 

 

or 

 

z = (x - X)/σ

 

where x is the individual laboratory result, X is the “Exercise Assigned Value,” and σ is the 

target value for the standard deviation. As described in the IUPAC guidelines, the choice of σ is 

dependent upon the data quality objective of a particular program. It can be fixed or determined 

by reference to validated methodology (e.g., the calculated σ from the exercise data, see Tables 4 

- 7. The fixed performance criterion is more useful in the comparison of a laboratory’s 

performance on different materials, while the use of the actual variation may be more useful 

within a given exercise, for example, if the determination of a particular analyte is more 

problematic than usual. 

The z-scores calculated using both approaches and applied to each laboratory’s data are given in 

Appendix A. The same criterion was adopted for use in this exercise as was used in the former 

NIST/NOAA/NS&T program, where the target standard deviation was set to 25 % of the 

exercise assigned value. The z-scores for the Control Material V represent 25 % of the assigned 

value so that Z = +1 is the assigned value plus 25 %, Z = -1 is the assigned value minus 25 % 

and so forth. Z-scores are also calculated based on the standard deviation of an analyte in the 

control material such that Z = +1 is one “exercise standard deviation” higher than the assigned 

value and Z = -1 is one “exercise standard deviation” lower than the assigned value and so forth. 

From a scientific point of view, IUPAC does not recommend the classification of z-scores, but 

does allow for such classification, e.g.,

⎪z⎪ ≤ 2 Satisfactory 

2 ≤ ⎪z⎪ ≤ 3 Questionable 

⎪z⎪ ≥ 3 Unsatisfactory 

The tables in Appendix A summarize the results and performance indices including the number 

of analytes that fall within each category for each laboratory. 

Precision Assessment (p-score):
13

 p = σlab / σtarget ≈ CVlab/ CVtarget

 

where σlab and σtarget are variance estimates for the individual laboratory and the target variance, 

respectively. The CVlab is the coefficient of variance (or ratio of standard deviation to the mean), 

while the CVtarget is a target value chosen by the participants. During the workshop that 

accompanied this exercise, a target CV of 15% was agreed upon, which is the same value used 

by the former NIST/NOAA/NS&T program. Note that the precision that p describes is that 

which occurs within a batch of analyses. Between batch variance is likely larger and was not 

assessed in this exercise. 

RESULTS AND DISCUSSION 

Summarized results are shown in Tables 4 through 7. The concentration of many 

organochlorines in Control Material V was considerably higher than in SRM 1945. The 

consensus value for the sum of PCB congeners in Control Material V (Table 3.1) was 8,240 ng/g 

wet mass versus 1,350 ng/g wet mass in SRM 1945 (sum of the certified and reference values 

Table 6). Likewise the sum of the organochlorine pesticides in Control Material V was 37,100 

ng/g wet mass versus 1,500 ng/g wet mass in SRM 1945 (sum of the reference values, Table 7). 

4,4’-DDE was present in the highest concentration in both samples with a consensus value of 

33,400 ng/g wet mass in Control Material V and certified value of 445 ng/g wet mass in SRM 

1945. The levels of total PCBs and organochlorine pesticides observed in Control Material V, 

while higher than SRM 1945, were in the range observed for California sea lions (Kajiwara et 

al., 2002). The mean “total extractable organics” determined in Control Material V was 6.42 % 

± 1.0 % (mean ± 95 % confidence interval; Table 4.1) and the average value determined by the 

participants in SRM 1945 was 71.8 % ± 2.6 % relative to the certified value of 74.3 % ± 0.45 % 

(Table 7). 

The relative scatter among the laboratories appeared similar for many of the compounds with 

some exceptions (Appendix B). As in previous years, this was especially noticeable for PCB 

congener 201 for both the control material and the SRM. This may be a result of the two 

nomenclature systems used for this PCB congener (Guitart et al., 1993). High biases (most 

values exceeding the certified value) were observed among the values determined in the SRM 

relative to the certified value for the following compounds: PCB 31, PCB 99, PCB 101/90, PCB 

138 (163+164), PCB 156, PCB 180, PCB 194, PCB 206, PCB 209, 4,4’-DDE, and 4,4’-DDD. 

Low biases (most values below the certified or reference value) were observed for PCB 18, PCB 

49, PCB 52, PCB 66, PCB 149, PCB 151, PCB 195, PCB 201, 4,4’-DDT, HCB, β-HCH, and 

trans-nonachlor. A number of laboratories reported results for analytes in addition to the target 

compounds (Appendix D). Additional analytes that were reported included other PCB congeners 

(Labs 1, 3, 4, 6, 8, 13, 17 and 18), coplanar PCBs (Labs 4 and 10), dioxins and furans (Lab 10), 

tris-(4-chlorophenyl) methane and methanol (Lab 10), other organochlorine pesticides (Labs 3 

and 4). 

The participants used a variety of different methods to analyze the materials in this exercise 

(Appendix E). Eleven laboratories used Soxhlet extraction to extract the materials, five used 

pressurized fluid extraction, and the remaining laboratories used other techniques including 
14

maceration with solvent and column elution. Of the laboratories that responded, eight 

laboratories performed a pre-separation on the sample extracts prior to GC analysis (i.e., 

fractionation), while nine laboratories did not. Eight laboratories used GC/MS to quantify the 

target analytes, while seven laboratories used GC-ECD. Four laboratories used a combination 

of the two techniques. Internal standards were used by 14 laboratories, while four laboratories 

used external standards. 

CONCLUSIONS 

During the Baltimore workshop there was some discussion regarding materials for future 

exercises. Blubber is typically the marine mammal tissue analyzed for organochlorine 

constituents; hence the program will continue to use this matrix. The exercise coordinators plan 

to vary the type of control material used to include other species of interest such as delphinids or 

other species. NIST is constrained somewhat on the choice of the material as a fairly large 

quantity (≈1 kg) is needed, and this amount of material is not available on a routine basis. SRM 

1945 will continue to be the SRM used in this exercise, since it is the only marine mammal tissue 

available with certified and reference values for organochlorine compounds. There was also 

discussion about including other analytes to the target list. For the 2003 exercise, 

polybrominated diphenyl ether congeners, toxaphene, and selected fatty acids were added as 

optional analytes. Participants were generally comfortable with the timing of the exercise. The 

timing of the 2003 exercise will be similar to the 2001 exercise, with sample material distributed 

to the participants in April 2003 and the results due 30 September 2003. A workshop will be 

held for the 2003 exercise in conjunction with the Biennial Conference on Biology of Marine 

Mammals to be held in Greensboro, North Carolina, in December 2003. 
15

 LITERATURE CITED 

Becker, P. R., Wise, S. A., Schantz, M. M., Koster, B. J., and Zeisler, R. “Alaska Marine 

Mammal Tissue Archival Project: Sample Inventory and Results of Analyses of Selected 

Samples for Organic Compounds and Trace Elements,” Rep. No. NISTIR 4731. U.S. Dept. 

of Commerce. (1992). 

Cantillo, A. Y. and Parris, R.M. National Status and Trends Program Quality Assurance Project: 

Trace Organic Intercomparison Exercise Results 1989-1990, NOAA Tech. Memo. 

NOS/ORCA 69 (1990) 

Cantillo, A. Y. and Parris, R. M. “Evaluation of Trace Organic NOAA Status and Trends Quality 

Assurance Project Performance,” in Quality Assurance for Analytical Laboratories, M. 

Parkany (ed.), Royal Society of Chemistry, M. Parkany (ed.), Royal Society of Chemistry, 

Special Publication No. 130 (1993) 

Cantillo, A. Y. NS&T Quality Assurance Project Intercomparison Results 1991-1995, NOAA 

Tech. Memo. 79 NOA/ORCA (1995). 

Guitart, R., Puig, P., and Gomez-Catalan, J. Requirement for a Standardized Nomenclature 

Criterion for PCBs: Computer-Assisted Assignment of Correct Congener Denomination and 

Numbering. Chemosphere 27:1451-1459. (1993). 

IUPAC “The International Harmonized Protocol for the Proficiency Testing of (Chemical) 

Analytical Laboratories,” Pure & Appl. Chem. 65:123-2144. (1993). 

Kajiwara, N., Kannan, K., Muroaka, M., Wantanabe, M., Takahashi, S., Gullard, F., Olsen, H., 

Blankership, A.L., Jones, P.D., Tanabe, S, and Giesy, J. Organochlorine pesticides, 

polychlorinated biphenyls, and butylin compounds in blubber and livers of stranded 

California sea lions, elephant seals, and harbor seals from coastal California, USA. Arch. 

Environ. Contam. Toxicol. 41:90-99 (2001). 

Kucklick, J. R., Christopher, S. J., Becker, P. R., Pugh, R. S., Porter, B. J., Schantz, M. M., 

Mackey, E. A., Wise, S. A., Rowles, T. K. Description and Results of the 2000 NIST/NOAA 

Interlaboratory Comparison Exercise Program for Organic Contaminants and Trace Elements 

in Marine Mammal Tissues. National Institute of Standards and Technology, NISTIR 6849, 

(2002). 

Kucklick, J. R., Tuerk, K. J., Vander Pol, S. S., Schantz, M. M., Wise, S. A. Polybrominated 

diphenyl ether congeners and toxaphene in selected marine standard reference materials. 

Anal. Bioanal. Chem. 378:1147-1151. (2004).

Motulsky, H. Grubbs' Test for Detecting Outliers. GraphPad Insight. Issue 14 (1997). 
16

Poster, D. L., Schantz, M. M., Kucklick, J. R., Lopez de Alda, M. J., Porter, B. J., Pugh, R., 

Wise, S. A. Three new mussel tissue standard reference materials (SRMs) for the 

determination of organic contaminants. Anal. Bioanal. Chem. 378: 1213-1231. (2004). 

Schantz, M. M., R. M. Parris, S.A. Wise, H. T. Won, and Turle, R. Polychlorinated biphenyl 

(PCB) congeners and chlorinated pesticides in a cod liver reference material. Chemosphere

24:1687-1698. (1992.). 

Schantz, M. M., Koster, B. J., Wise, S. A., and Becker, P. R. Determination of PCBs and 

Chlorinated Hydrocarbons in Marine Mammal Tissues. Sci. Total Environ. 139/140:323-345. 

(1993). 

Schantz, M. M., Koster, B. J., Oakley, L. M., Schiller, S. B., and Wise, S. A. Certification of 

Polychlorinated Biphenyl Congeners and Chlorinated Pesticides in a Whale Blubber 

Standard Reference Material. Anal. Chem. 34:901-910. (1995). 

Schantz, M. M., Wise, S. A., Segstro, M., Muir, D. C. G., and Becker, P. R. Interlaboratory 

Comparison Study for PCB Congeners and Chlorinated Pesticides in Beluga Whale Blubber. 

Chemosphere 33:1369-1390. (1996). 

Schantz, M. M., Demiralp, R., Greenberg, R. R., Hays, M. J., Parris, R. M., Porter, B. J., Poster, 

D. L., Sander, L. C., Sharpless, K. S., Wise, S. A., and Schiller, S. B. Certification of a 

Frozen Mussel Tissue Standard Reference Material (SRM 1974a) for Trace Organic 

Constituents. Fresenius J. Anal. Chem. 358:431-440. (1997). 

Schantz, M. M., Porter, R. M., and Wise, S. A. NIST/NOAA NS&T Intercomparison Exercise 

Program for Organic Contaminants in the Marine Environment. Description and Results of 

1999 Organic Intercomparison Exercise. NOAA Tech. Memo. 146 NOA/ORCA (1999). 

Schantz, M. M., Kucklick, J.R., Parris, R. M., and Wise, S.A. NIST Intercomparison Exercise 

Program for Organic Contaminants in the Marine Environment. Description and Results of 

2000 Organic Intercomparison Exercise. National Institute of Standards and Technology 

NISTIR 6837. (2002). 

Wise, S. A., Schantz, M. M., Koster, B. J., Demiralp, R., Mackey, E. A., Greenberg, R. R., 

Burow, M., Ostapczuk, P., and Lillestolen, T. I. Development of Frozen Whale Blubber and 

Liver Reference Materials for the Measurement of Organic and Inorganic Contaminants. 

Fresenius J. Anal. Chem. 345:270-277. (1993). 

Wong C.S., Hoekstra, P.F., Karlsson, H., Backus, S.M., Mabury, S.A., and Muir, D.C. 

Enantiomer fractions of chiral organochlorine pesticides and polychlorinated biphenyls in 

standard and certified reference materials. Chemosphere 49:1339-47 (2002). 

Wise, S. A. Quality Assurance of Contaminant Measurements in Marine Mammal Tissues. 

Coastal Zone 93: Proceedings of the 8th Symposium on Coastal and Ocean Management 
17

(New Orleans, LA), O.T. Magoon, W.S.Wilson, H. Converse, and L.T. Tobin, editors. 

ASCE, New York, NY. 3:2531-2541 (1993). 

Zeisler, R., Langland, J. K., and Harrison, J. K. Cryogenic Homogenization Procedure for 

Biological Tissues. Anal. Chem. 60: 2760-2765. (1983). 
Marine Mammal QA Material V Laboratory Reported Values 

PCB Congener 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 Mean 1 18 7.31 <8.2 7.58 9.28 9.89 4.77 54.3 NA 11.7 NA <LOD <LOD 7.40 <10 NA 5.53 6.12 NA 12.4 1

28 4.12 <8.2 2.64 3.35 NA 4.93 1.67 2.05 41.2 coelution 15.6 <LOD 2.07 <10 3.05 4.57 2.85 NA 7.33 1

31 coelution NA <1.83 <LOD NA 2.145 <1 9.85 NA coelution 2.89 NA <1.1 NA 0.717 2.37 1.73 NA 3.28 344 35.7 30.0 38.1 42.6 37.8 44.8 17.7 36.0 29.1 28.4 49.5 28.8 39.7 41.3 NA 29.0 36.0 NA 35.3 749 90.9 76.0 98.5 105 88.1 107 43.7 88.1 NA 84.3 105 93.1 97.7 110 38.5 65.0 coelution NA 86.1 252 145 128 154 146 134 NA 63.0 134 158 111 165 144 153 170 132 113 140 163 138 2

66/95* 197 203 208.3 246 193 192 <1 NA 107 142 202 213 NA 243 NA 146 203 see below 192 387 151 202 168 NA NA NA NA NA NA 107 170 144 180 193 0.142 123 126 NA 142 599 455 508 431 coelution 404 564 NA 468 NA 409 599 465 470 538 NA 370 430 430 467 6101 (+90) 419 261 472 coelution 381 540 190 471 577 278 476 443 493 369 NA 293 416 477 410 1105 154 139 163 200 166 189 67.0 180 144 177 196 127 167 255 90 173 137 170 161 4118 527 650 541 581 537 477 243 555 496 474 629 648 627 590 177 477 422 540 511 1128 142 175 187 243 172 222 80.7 196 215 98 231 262 NA 165 146 167 145 NA 178 5132 coelution NA NA NA 108 NA NA NA NA 225 NA 262 NA NA NA 91.0 114 NA 160 7138 (+163+164) 1098 398 1563 1173 1003 NA 533 1100 1357 1300 1289 1203 1533 1567 117 863 coelution 1567 1104 4149 307 437 334 386 334 503 147 328 NA 229 271 290 380 NA NA 270 269 313 320 8151 133 151 140 280 136 193 59.0 140 NA 80 137 109 160 126 NA 217 114 120 143 5153 2392 2490 2123 1473 1536 NA 840 1985 1867 1847 1851 1805 2233 2008 119 1500 1506 1867 1732 5

156 10.2 <11.1 6.6 <LOD <LOD 6.98 <1 31.4 NA 7.8 19.8 67.3 31.0 101 8.70 6.37 7.64 NA 25.4 3170 (+190) 236 110 268 262 235 249 117 283 223 195 285 167 260 273 114 233 185 233 218 5180 775 1111 964 671 731 1028 383 882 770 853 1649 696 877 729 NA 717 coelution 787 851 2183 225 240 237 314 218 280 106 NA NA 220 329 14.2 257 207 NA 197 162 243 217 7187 447 532 491 455 NA NA 223 662 394 380 540 204 463 484 NA 387 332 550 436 1194 160 161 182 209 158 212 49.3 178 NA 120 209 126 157 150 NA 157 124 NA 157 4

195 38.3 40.0 42.3 46.9 NA 42.3 NA NA 30.4 21.6 108 26.5 NA 81.2 NA 40.0 30.4 NA 45.7 2201 38.3 44.0 NA NA 40.5 261 NA NA NA coelution 50.4 177.0 NA 57.7 NA 90.0 30.5 NA 87.7 7206 79.5 70.0 93.3 91.2 82.2 109 NA NA 68.4 NA 101.7 71.2 NA 81.7 NA 84.3 64.7 NA 83.1 1209 84.7 51.0 60.9 87.2 61.2 79.3 NA 69.5 70.7 NA 82.9 75.4 NA 54.7 NA NA 55.2 NA 69.4 166 55.5 57.0 61.3 52.8 48.5 37.9 NA NA NA 52.4 42.5 83.8 NA NA 54.4 41.7 53.5 NA 53.4 1

95 141 146 147 193 144 154 NA NA NA 89.9 159 129 167 NA NA 104 150 113.33 141 2NA: Data were not availableOutliers are in Bold

Table 4: PCB Congeners in Control Material V (ng/g wet mass) Reported by the Participants the NIST/NOAA Interlaboratory Comparison Exercise ProgrMarine Mammal Tissues
Marine Mammal QA Material V Laboratory Reported Values 

Congener 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 Mean 1 SD 2,4’-DDT 57.4 51.3 63.1 172 71.3 NA 167 65.2 66.4 NA 62.2 NA 66.7 NA 102 70.0 65.8 NA 83.1 40 

4,4’-DDT 182 196 150 217 202 NA 96 206 252 206 148 204 137 NA 223 140 195 35 174 54 

2,4’-DDE 115 105 112 151 113 NA 29.7 98.3 160 NA 121 NA 78.7 NA 9.20 210 124 NA 110 52 4,4’-DDE 45695 41700 37067 31300 26000 NA 18676 32895 25367 50467 26654 52774 37667 NA 39100 5500 40888 22667 33401 1234

2,4’-DDD 37.8 28.0 41.9 109.5 50.6 NA 30.0 NA 324 NA 68.4 NA 36.0 NA 28.0 39.0 33.7 NA 68.9 83 

4,4’-DDD 1602 834 1070 1123 NA NA 483 1096 1057 951 756 1144 706 NA 1060 1000 1191 NA 1005 263 

HCB <1 <17 <2.39 1.29 0.140 NA <1 0.085 2.36 <5 2.96 NA <1.1 NA <5 4.50 <5 NA 1.89 1.7 

alpha-HCH 9.08 <8.9 7.47 coelution 5.55 NA 4.33 3.94 6.57 7.75 9.24 5.32 6.03 NA 7.10 4.80 <5 NA 6.43 1.7 beta-HCH 454 <59 441 328 341 NA NA 325 433 370 428 382 360 NA 460 590 interference NA 409 75 

gamma-HCH 7.74 <46 7.79 4.54 6.01 NA 3.00 6.12 8.03 7.00 11.4 4.69 30.3 NA <5 3.60 12.50 NA 8.67 7.1 

Heptachlor Epoxide 115 67.3 115 97.7 108 NA NA NA 65.3 102 101 NA NA NA 150 43.0 134 NA 99.8 31 Cis-Chlordane 31.0 45.3 41.5 coelution 44.4 NA 23.7 33.1 34.2 35.9 32.4 32.6 45.3 NA 80.0 26.0 61.3 NA 40.5 15 Trans-Chlordane 32.2 <41 3.17 NA 10.9 NA 4.3 1.32 5.80 9.6 <LOD <LOD 2.77 NA <5 5.70 39.5 NA 11.5 13 Oxychlordane 78.3 <164 79.3 73.9 58.1 NA NA 74.1 77.2 78.0 89.0 104 NA NA 99.0 70.0 66.1 NA 78.9 13 Cis-Nonachlor 95.3 120.0 119.3 39.1 NA NA NA 231 110 120 57.1 118 NA NA 107 79.0 118 NA 109 47 

Trans-Nonachlor 1323 1400 1213 968 1008 NA 657 1208 1193 1387 1030 1745 NA NA 1660 1100 1436 NA 1238 285 

Dieldrin 252 268 238 348 165 NA <1 NA 19.3 247 261 NA NA NA 65.0 180 252 NA 209 95 

Mirex 40.9 48.7 47.7 67.8 40.9 NA NA NA 41.3 NA 54.1 NA NA NA 29.0 25.0 42.0 NA 43.7 12 

Lipid (%) 6.17 6.33 6.00 0.659 6.13 6.98 3.40 6.84 8.80 6.59 4.93 7.44 21.7 7.67 8.04 6.60 6.54 10.00 7.27 4.1 NA: Data were not availableOutliers are in Bold

Table 5: Organochlorine Pesticides in Control Material V (ng/g wet mass) Reported by the Participants in the NIST/NOAA Interlaboratory Comparison ExeContaminants in Marine Mammal Tissues
PCB SRM 1945 Laboratory 

Congener 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 Mean 1 SD 95% CI n 18 3.25 <8.2 3.71 24.2 4.13 1.81 3.00 NA <2 NA 1.53 <LOD 2.90 3.80 NA 2.63 5.03 NA 5.09 6.4 3.8 11 28 15.0 11.5 10.2 11.4 NA 15.2 15.3 8.33 35.2 coelution 14.0 14.2 10.8 18.3 14.7 11.0 12.7 NA 14.5 6.3 3.2 15 31 coelution NA 4.77 5.70 NA 4.92 2.00 5.55 NA coelution 3.07 NA 4.37 NA 5.04 3.27 3.25 NA 4.19 1.2 0.8 10 44 11.2 10.6 12.4 13.1 14.1 13.0 <1 12.1 14.3 9.32 13.4 7.68 13.3 16.3 NA 10.3 11.7 NA 12.2 2.2 1.1 15 49 15.9 13.3 18.5 22.1 19.3 17.9 1.50 16.7 NA 15.6 16.6 14.7 19.7 20.5 7.58 21.3 coelution NA 16.1 5.4 2.7 15 52 37.6 35.7 41.9 36.0 39.2 NA 18.7 34.0 37.0 29.1 37.4 32.0 42.3 42.1 38.3 36.3 42.9 39.3 36.5 5.9 2.8 17 66/95* 56.3 51.7 62.4 66.8 62.7 50.4 <1 NA 22.2 43.9 61.6 51.6 NA 64.5 NA 52.3 57.0 see below 54.1 12 6.3 13 87 23.1 22.0 26.3 NA NA NA NA NA NA 15.1 23.8 17.6 29.7 25.0 10.4 17.0 16.0 NA 20.5 5.7 3.4 11 99 56.9 55.0 62.6 coelution 62.5 62.3 NA 54.0 NA 51.4 67.4 49.1 54.0 89.0 NA 51.0 49.8 55.3 58.6 10 5.4 14 101 (+90) 77.9 41.3 93.7 coelution 75.5 88.7 63.7 79.7 115 50.5 88.2 68.1 81.7 64.0 NA 63.0 69.8 76.0 74.8 17 8.6 16 105 30.1 19.7 28.7 27.5 26.4 27.5 43.3 28.8 45.2 27.8 29.2 18.1 31.0 41.3 16.3 47.3 29.2 21.0 29.9 9.0 4.2 18 118 79.0 81.7 80.8 87.4 83.4 63.9 89.7 80.1 71.9 71.3 79.6 80.0 79.7 77.5 59.5 76.7 73.8 70.3 77.0 7.6 3.5 18 128 23.1 <55 23.9 136 21.7 23.8 24.3 22.9 30.2 14.0 39.9 10.3 NA 33.0 20.0 24.7 19.8 NA 31.2 30 15 15 132 coelution NA NA NA 16.5 NA NA NA NA 30.1 NA 27.6 NA NA NA 18.3 17.4 NA 22.0 6.4 5.6 5 138 (+163+164) 125 48.7 190 166 135 NA 163 140 127 158 209 118 180 162 71.6 123 coelution 217 146 45 22 16 149 73.4 82.7 88.2 90.3 83.1 132 74.7 78.8 NA 54.0 75.9 54.6 85.0 NA NA 80.3 95.0 65.0 80.8 18 9.3 15 151 26.8 24.7 27.7 114 24.8 40.2 24.7 25.3 NA 15.5 31.4 16.3 31.3 29.0 NA 24.0 27.5 19.7 31.5 23 11 16 153 251 238 240 185 191 NA 213 220 171 200 273 162 223 238 150 187 215 213 210 33 16 17 156 14.4 <11.1 11.5 12.3 10.4 10.7 19.3 13.2 NA 10.6 17.1 18.9 15.3 45.0 14.9 12.3 9.6 NA 15.7 8.7 4.4 15 170 (+190) 48.3 19.0 49.6 40.3 43.6 34.8 43.7 46.2 58.2 30.9 48.4 22.7 46.7 49.5 28.4 41.3 40.2 40.3 40.7 10 4.7 18 180 134 150 149 135 134 163 163 155 101 146 170 101 143 125 NA 133 coelution 167 142 21 10 16 183 40.7 35.3 41.3 63.1 36.5 44.3 47.0 NA NA 36.1 109.7 NA 42.0 42.0 NA 33.3 34.9 47.0 46.7 20 10 14 187 119 120 127 136 NA NA 120 178 100 104 136 28.1 117 106 NA 107 96.0 173 118 35 18 15 194 57.7 57.7 60.4 71.8 55.1 64.5 38.7 59.6 NA 42.8 55.8 33.1 53.0 45.0 NA 57.3 38.7 NA 52.7 11 5.5 15 195 10.7 10.0 10.4 9.19 NA 10.7 NA NA 2.00 5.69 30.5 5.18 NA 23.0 NA 12.0 16.7 NA 12.2 7.9 4.5 12 201 18.7 13.0 NA NA 24.7 97.6 NA NA 2.60 coelution 16.0 52.2 NA 25.0 NA 41.3 17.0 NA 30.8 27 17 10 206 48.9 38.3 48.4 41.5 47.7 56.4 NA NA 28.3 NA 41.6 38.1 NA 33.0 NA 65.3 35.3 NA 43.6 10 5.9 12 209 21.8 15.3 15.7 25.4 20.0 20.4 NA 19.1 8.57 NA 17.0 21.1 NA 13.0 NA NA 10.3 NA 17.3 5.0 2.8 12 66 22.0 18.7 23.7 21.0 20.1 15.2 NA NA NA 21.6 18.8 25.2 NA NA 20.0 21.7 22.7 NA 20.9 2.6 1.5 12 95 34.3 33.0 38.6 45.9 42.6 35.3 NA NA NA 22.3 42.8 26.5 42.0 NA NA 30.7 34.3 32.00 35.4 6.9 3.7 13 NA: Data were not available

Table 6: PCB Congeners in SRM 1945 (ng/g wet mass) Reported by the Participants in the NIST/NOAA Interlaboratory Comparison Exercise Program for Mammal Tissues
SRM 1945 Laboratory R

Compound 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 Mean s 95% CI n 2,4’-DDT 71.7 52.3 79.9 81.5 92.9 NA 123 83.0 62.2 NA 95.3 NA 68.3 NA 57.7 117 96.2 NA 84.1 22 12 13 

4,4’-DDT 229 158 181 217 243 NA 230 254 125 276 237 311 173 NA 162 183 232 46.7 213 50 25 15 

2,4’-DDE 14.9 11.3 15.1 38.5 7.30 NA 19.7 12.3 18.9 NA 19.4 NA 11.0 NA 104 8.30 12.8 NA 23.2 26 14 13 

4,4’-DDE 529 549 550 507 439 NA 510 479 442 714 545 598 523 NA 377 490 465 357 513 78 39 15 

2,4’-DDD 20.2 <15 25.8 25.8 26.5 NA 24.3 NA 63.7 NA 19.9 NA 17.3 NA 10.7 18.3 17.3 NA 25.0 14 8.2 11 

4,4’-DDD 114 58.3 152 112 NA NA 103 112 90.6 130 105 126 82.7 NA 85.6 117 126 NA 108 24 12 14 

HCB 25.6 21.7 30.5 33.8 23.1 NA 23.3 24.8 26.0 NA 29.8 24.9 32.7 NA 17.9 22.3 30.6 NA 26.3 4.6 2.4 14 

alpha-HCH 13.7 19.0 18.0 coelution 13.4 NA 28.7 12.4 18.7 15.1 12.1 11.1 15.0 NA 15.4 8.83 16.0 NA 15.7 4.8 2.5 14 beta-HCH 3.46 <59 8.36 NA 1.91 NA NA 1.90 <2 1.37 4.72 <LOD 1.93 NA <10 4.07 interference NA 3.47 2.3 1.6 8 gamma-HCH 2.35 NA 3.41 11.8 2.43 NA 19.7 2.58 7.66 2.64 7.82 1.74 9.50 NA <5 2.23 3.31 NA 6.23 5.3 2.9 13 Heptachlor Epoxide 12.2 <8 12.8 14.7 9.75 NA NA NA 4.00 11.9 13.1 NA NA NA 14.7 4.63 10.5 NA 10.7 3.8 2.3 10 Cis-Chlordane 51.2 47.3 58.3 coelution 45.1 NA 51.7 47.2 39.1 54.4 60.7 47.1 53.0 NA 43.7 42.0 48.4 NA 49.1 6.1 3.2 14 Trans-Chlordane 11.0 10.5 13.5 19.3 13.9 NA 24.0 11.2 8.83 13.8 12.7 11.1 11.0 NA <5 9.10 11.3 NA 13.1 4.1 2.2 14 Oxychlordane 21.7 <164 22.2 20.9 21.6 NA NA 23.4 13.8 18.8 24.5 21.4 NA NA 21.1 21.7 19.1 NA 20.8 2.7 1.5 12 Cis-Nonachlor 46.1 49.0 57.7 13.7 NA NA NA 98.0 24.5 55.1 50.4 47.7 NA NA 57.7 38.7 48.0 NA 49.1 20 12 12 Trans-Nonachlor 179 166 180 161 166 NA 147 166 138 223 178 180 NA NA 163 163 208 NA 172 22 12 14 

Dieldrin 54.1 <62 58.7 69.7 48.6 NA 227 NA 30.7 59.5 55.5 NA NA NA 18.3 42.7 39.0 NA 65.0 56 33 11 

Mirex 31.3 35.3 36.4 37.8 31.5 NA NA NA 29.2 NA 34.9 NA NA NA 19.8 8.50 28.9 NA 29.1 9.0 5.6 10 Lipid (%) 69.0 68.7 55.1 66.8 75.6 76.5 71.7 73.6 76.1 73.0 67.3 69.0 74.3 73.6 74.5 78.4 74.4 74.5 71.8 5.4 2.6 17 NA: Data were not available

Table 7: Organochlorine Pesticides in SRM 1945 (ng/g wet mass) Reported by the Participants in the NIST/NOAA Interlaboratory Comparison Exercise Contaminants in Marine Mammal Tissues
APPENDIX A 

Tabular results of PCB congener, pesticide, and lipid data reported by all laboratories.
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQAV AND SRM 1945

data are reported as if three figures are significant

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 7/19/2001 7/19/2001 7/20/2001 7/19/2001 7/19/2001 7/19/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

2,4’-DDT 57.2 57.0 57.9 73.6 67.8 73.9 57.4 0.8 71.7 4.8 83.1 22 106 14 -14,4’-DDT 182 184 180 232 226 231 182 1.0 229 1.4 184 21 245 15 0

2,4’-DDE 117 115 114 14.7 14.9 15.2 115 1.4 14.9 1.9 110 28 12.3 0.87 04,4’-DDE 44798 48538 43748 538 522 528 45695 5.5 529 1.5 33401 6050 445 37 12,4’-DDD 37.6 39.2 36.6 20.2 20.1 20.2 37.8 3.4 20.2 0.5 45.7 14 18.1 2.8 -04,4’-DDD 1605 1559 1641 116 112 114 1602 2.6 114 1.8 1005 138 133 10 2HCB <1 <1 <1 24.6 26.4 25.8 <1 <1 25.6 3.6 1.89 1.4 32.9 1.7α-HCH 9.70 8.34 9.20 13.4 13.9 13.6 9.08 7.6 13.7 1.9 6.43 1.0 16.2 3.4 1

β-HCH 457 452 452 3.24 3.54 3.59 454 0.6 3.46 5.6 409 42.5 8.00 1.4 0γ-HCH 7.36 8.22 7.64 2.67 2.17 2.23 7.74 5.7 2.35 11.5 6.87 1.64 3.30 0.81 0

Heptachlor Epoxide 113 118 114 11.8 12.1 12.6 115 2.5 12.2 3.3 99.8 18 10.8 1.3 0Cis-Chlordane 32.0 33.3 27.7 49.4 51.9 52.4 31.0 9.5 51.2 3.1 37.4 5.4 46.9 2.8 -0Trans-Chlordane 33.0 32.0 31.6 10.5 11.3 11.4 32.2 2.2 11.0 4.2 11.5 8.2 12.9 3.5 7Oxychlordane 81.2 77.5 76.3 22.6 21.4 21.2 78.3 3.3 21.7 3.5 78.9 7.4 19.8 1.9 0Cis-Nonachlor 94.2 96.1 95.6 44.6 45.4 48.5 95.3 1.0 46.1 4.4 98.4 17 48.7 7.6 -0Trans-Nonachlor 1333 1310 1327 175 182 180 1323 0.9 179 2.0 1238 150 231 11 0Dieldrin 255 255 246 55.6 53.5 53.3 252 1.9 54.1 2.3 209 56 37.5 3.9 0Mirex 39.2 42.3 41.3 30.0 31.6 32.1 40.9 3.8 31.3 3.6 43.7 7.5 18.9 2.8 -0

Category z (2

≤ 2 1

2 to 3 

≥ 3 

z (2

Lipid (mass fraction (%)) 5.98 6.30 6.23 69.5 68.4 69.2 6.2 2.73 69.0 0.82 6.4 2.08 74.3 0.45 -0

*Certified values are in bold **See text for explaination

Homog. V SRM 1945 SRM 1945 Homog. V

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass)

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQA V AND SRM 1945

data are reported as if three figures are significant

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 7/19/2001 7/19/2001 7/20/2001 7/19/2001 7/19/2001 7/19/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

18 7.11 7.29 7.54 3.29 3.28 3.18 7.31 3.0 3.25 1.8 7.73 1.5 4.48 0.88 -028 3.96 4.09 4.32 15.0 15.4 14.6 4.12 4.5 15.0 2.5 4.26 2.3 14.10 1.4 -031 coelution coelution coelution coelution coelution coelution 3.28 2.6 3.12 0.6944 35.9 35.9 35.3 10.9 11.9 10.7 35.7 1.0 11.2 5.8 35.3 3.8 12.2 1.4 049 90.5 91.0 91.1 16.1 16.2 15.4 90.9 0.4 15.9 2.9 86.1 11 20.8 2.8 052 144 146 144 37.1 38.8 37.0 145 0.7 37.6 2.8 143 8 43.6 2.5 066/95* see below see below see below see below see below see below 192 21 57.4 2.587 151 153 149 22.5 23.9 22.9 151 1.4 23.1 3.1 157 20 16.7 1.4 -099 456 463 448 57.5 56.7 56.3 455 1.7 56.9 1.1 467 34 45.4 5.4 -0101 (+90) 417 426 412 78.9 77.3 77.4 419 1.6 77.9 1.2 410 53 65.2 5.6 0105 155 156 152 29.9 28.9 31.5 154 1.4 30.1 4.4 161 19 30.1 2.3 -0118 528 536 519 79.4 77.3 80.2 527 1.7 79.0 1.9 511 59 74.6 5.1 0128 145 142 139 22.4 22.4 24.5 142 1.8 23.1 5.2 178 25 23.7 1.7 -0132 coelution coelution coelution coelution coelution coelution 160 68 29.1 6.0138 (+163+164) 1101 1149 1044 125 122 127 1098 4.8 125 2.2 1104 213 131.5 7.4 0149 305 316 301 73.2 73.4 73.6 307 2.6 73.4 0.3 320 43 107 8.4 -0151 132 137 130 26.1 27.4 27.0 133 2.5 26.8 2.5 134 20 28.7 5.2 0153 2369 2482 2326 253 249 252 2392 3.4 251 0.9 1833 199 213.0 19 1156 10.2 10.0 10.6 14.5 14.0 14.7 10.2 3.2 14.4 2.7 18.5 11 10.3 1.1 -1170 (+190) 236 230 242 47.5 46.2 51.1 236 2.5 48.3 5.2 218 27 40.6 2.6 0180 779 782 764 134 128 141 775 1.2 134 5.0 798 87 107 5.3 -0183 226 229 219 40.1 39.8 42.2 225 2.5 40.7 3.2 231 30 36.6 4.1 -0187 451 457 433 121 116 122 447 2.8 119 2.6 436 62 105 9.1 0194 167 154 160 57.1 56.1 60.0 160 4.0 57.7 3.5 165 16 39.6 2.5 -0195 39.7 37.9 37.2 11.1 10.5 10.5 38.3 3.5 10.7 3.4 40.0 9 17.7 4.3 -0201 37.9 38.4 38.5 17.9 18.6 19.7 38.3 0.8 18.7 4.9 87.7 52 17.0 0.89 -2206 82.2 78.9 77.2 48.0 47.7 51.0 79.5 3.2 48.9 3.7 83.1 7.8 31.1 2.7 -0

209 81.1 83.8 89.1 21.2 21.4 22.8 84.7 4.8 21.8 3.8 69.4 7.2 10.6 1.1 066 56.1 55.1 55.4 22.0 21.8 22.2 55.5 0.9 22.0 0.9 50.7 4 23.6 1.6 095 141 144 139 33.9 35.2 33.8 141 1.7 34.3 2.3 141 15 33.8 1.7 0

*Certified values are in bold **See text for explaination Category z (2

≤ 2 2

2 to 3 

≥ 3 

Homog. V SRM 1945 Homog. V SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass) 

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQAV AND SRM 1945

data are reported as if three figures are significant

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 10/2/2010 10/2/2001 10/2/2001 10/2/2001 10/2/2001 10/2/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

2,4’-DDT 60.0 45.0 49.0 50.0 55.0 52.0 51.3 15 52.3 4.8 83.1 22 106 14 -14,4’-DDT 226 184 179 142 172 161 196 13 158 9.6 184 21 245 15 0

2,4’-DDE 112 103 99.0 12.0 11.0 11.0 105 6.4 11.3 5.1 110 28 12.3 0.87 -04,4’-DDE 46000 40200 38900 552 547 547 41700 9.1 549 0.5 33401 6050 445 37 12,4’-DDD 32.0 27.0 25.0 <15 <15 <15 28.0 13 <15 <15 45.7 14 18.1 2.8 -14,4’-DDD 1010 777 715 63.0 58.0 54.0 834 19 58 7.7 1005 138 133 10 -0HCB <17 <17 <17 18.0 23.0 24.0 <17 <17 21.7 15 1.89 1.4 32.9 1.7α-HCH <8.9 <8.9 <8.9 18.0 18.0 21.0 <8.9 <8.9 19.0 9.1 6.43 1.0 16.2 3.4

β-HCH <59 <59 <59 <59 <59 <59 <59 <59 <59 <59 409 42.5 8.00 1.4γ-HCH <46 <46 <46 <46 <46 <46 <46 <46 <46 <46 6.87 1.64 3.30 0.81

Heptachlor Epoxide 71.0 70.0 61.0 <8 <8 <8 67.3 8.2 <8 <8 99.8 18 10.8 1.3 -1Cis- Chlordane 47.0 45.0 44.0 44.0 49.0 49.0 45.3 3.4 47.3 6.1 37.4 5.4 46.9 2.8 0Trans- Chlordane <41 <41 <41 <41 11.0 10.0 <41 <41 10.5 6.7 11.5 8.2 12.9 3.5

Oxychlordane <164 <164 <164 <164 <164 <164 <164 <164 <164 <164 78.9 7.4 19.8 1.9

Cis- Nonachlor 138.0 111.0 111.0 44.0 51.0 52.0 120.0 13.0 49.0 8.9 98.4 17 48.7 7.6 0Trans- Nonachlor 1490 1370 1340 164 170 164 1400 5.7 166 2.1 1238 150 231 11 0Dieldrin 293 259 251 <62 <62 <62 268 8.3 <62 <62 209 56 37.5 3.9 1Mirex 55.0 46.0 45.0 35.0 35.0 36.0 48.7 11.3 35.3 1.6 43.7 7.5 18.9 2.8 0

Category z (2

≤ 2 1

2 to 3 

≥ 3 

z (2

Lipid (mass fraction (%)) 6.40 6.50 6.10 69.0 68.0 69.0 6.3 3.29 68.7 0.84 6.4 2.08 74.3 0.45 -0

*Certified values are in bold **See text for explaination

Homog. V SRM 1945 SRM 1945 Homog. V

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass)

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQA V AND SRM 1945

data are reported as if three figures are significant

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 10/2/2001 10/2/2001 10/2/2001 10/2/2001 10/2/2001 10/2/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

18 <8.2 <8.2 <8.2 <8.2 <8.2 <8.2 <8.2 <8.2 <8.2 <8.2 7.73 1.5 4.48 0.88

28 <8.2 <11 <11 <11.3 11.5 <11.3 <8.2 <8.2 11.5 4.26 2.3 14.10 1.431 NA NA NA NA NA NA NA NA NA NA 3.28 2.6 3.12 0.6944 30.0 34.0 33.0 10.6 <10.5 <10.5 32.3 6.4 10.6 35.3 3.8 12.2 1.4 -049 76.0 82.0 81.0 14.0 13.0 13.0 79.7 4.0 13.3 4.3 86.1 11 20.8 2.8 -052 128 139 136 37.0 35.0 35.0 134 4.2 35.7 3.2 143 8 43.6 2.5 -066/95* see below see below see below see below see below see below 192 21 57.4 2.587 202 177 181 22.0 22.0 22.0 187 7.2 22.0 0.0 157 20 16.7 1.4 099 508 462 460 55.0 53.0 57.0 477 5.7 55.0 3.6 467 34 45.4 5.4 0101 (+90) 261 237 237 41.0 42.0 41.0 245 5.7 41.3 1.4 410 53 65.2 5.6 -1105 139 129 163 22.0 18.0 19.0 144 12.2 19.7 10.6 161 19 30.1 2.3 -0118 650 524 554 79.0 81.0 85.0 576 11.4 81.7 3.7 511 59 74.6 5.1 0128 175 183 182 <55 <55 <55 180 2.4 <55 <55 178 25 23.7 1.7 0132 NA NA NA NA NA NA NA NA NA NA 160 68 29.1 6.0138 (+163+164) 398 367 464 55 45 46 410 12.1 49 11.3 1104 213 131.5 7.4 -2149 437 369 360 82.0 83.0 83.0 389 10.8 82.7 0.7 320 43 107 8.4 0151 151 133 131 25.0 25.0 24.0 138 8.0 24.7 2.3 134 20 28.7 5.2 0153 2490 2100 2100 237 239 239 2230 10.1 238 0.5 1833 199 213.0 19 0156 <11.1 <11.1 <11.1 <11 <11 <11 <11 <11 <11 <11 18.5 11 10.3 1.1170 (+190) 110 113 115 20.0 18.0 19.0 113 2.2 19.0 5.3 218 27 40.6 2.6 -1180 1111 891 926 148 150 153 976 12.1 150 1.7 798 87 107 5.3 0

183 240 254 252 39.0 33.0 34.0 249 3.0 35.3 9.1 231 30 36.6 4.1 0187 532 439 444 117 120 123 472 11.1 120 2.5 436 62 105 9.1 0194 161 169 168 61.0 57.0 55.0 166 2.6 57.7 5.3 165 16 39.6 2.5 0195 40.0 40.0 39.0 <7 9.0 11.0 39.7 1.5 10.0 14.1 40.0 9 17.7 4.3 0201 44.0 37.0 35.0 14.0 13.0 12.0 38.7 12.2 13.0 7.7 87.7 52 17.0 0.89 -2206 70.0 74.0 75.0 42.0 35.0 38.0 73.0 3.6 38.3 9.2 83.1 7.8 31.1 2.7 -0

209 51.0 56.0 52.0 16.0 15.0 15.0 53.0 5.0 15.3 3.8 69.4 7.2 10.6 1.1 -066 57.0 53.0 53.0 18.0 19.0 19.0 54.3 4.3 18.7 3.1 50.7 4 23.6 1.6 095 146 142 142 34.0 33.0 32.0 143 1.6 33.0 3.0 141 15 33.8 1.7 0

*Certified values are in bold **See text for explaination Category z (2

≤ 2 2

2 to 3 

≥ 3 

Homog. V SRM 1945 Homog. V SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass) 

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQAV AND SRM 1945

data are reported as if three figures are significant

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 7/11/2001 7/11/2001 7/11/2001 7/11/2001 7/11/2001 7/11/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

2,4’-DDT 63.5 63.1 62.8 80.9 78.8 80.0 63.1 0.6 79.9 1.3 83.1 22 106 14 -14,4’-DDT 160 134 157 187 177 179 150 9.5 181 2.9 184 21 245 15 -0

2,4’-DDE 112 112 111 15.3 14.9 15.0 112 0.5 15.1 1.4 110 28 12.3 0.87 04,4’-DDE 37000 37500 36700 567 545 539 37067 1.1 550 2.7 33401 6050 445 37 02,4’-DDD 42.3 41.5 42.0 25.7 25.8 25.8 41.9 1.0 25.8 0.2 45.7 14 18.1 2.8 -04,4’-DDD 1070 1080 1060 152 152 153 1070 0.9 152 0.4 1005 138 133 10 0HCB <1.78 <2.39 <2.13 30.2 31.3 29.9 <2.39 <2.13 30.5 2.4 1.89 1.4 32.9 1.7α-HCH 7.53 7.49 7.39 17.5 18.9 17.7 7.47 1.0 18.0 4.2 6.43 1.0 16.2 3.4 0

β-HCH 436 446 441 6.57 9.05 9.46 441 1.1 8.36 18.7 409 42.5 8.00 1.4 0γ-HCH 8.57 7.45 7.35 3.60 3.33 3.31 7.79 8.7 3.41 4.7 6.87 1.64 3.30 0.81 0

Heptachlor Epoxide 114 116 114 12.6 13.3 12.5 115 1.0 12.8 3.4 99.8 18 10.8 1.3 0Cis- Chlordane 41.7 41.8 41.0 58.0 59.0 57.9 41.5 1.1 58.3 1.0 37.4 5.4 46.9 2.8 0Trans- Chlordane 3.15 2.92 3.44 13.3 13.5 13.6 3.17 8.2 13.5 1.1 11.5 8.2 12.9 3.5 -2Oxychlordane 78.1 80.3 79.4 23.1 22.0 21.6 79.3 1.4 22.2 3.5 78.9 7.4 19.8 1.9 0Cis- Nonachlor 119.0 120.0 119.0 57.7 57.6 57.7 119.3 0.5 57.7 0.1 98.4 17 48.7 7.6 0Trans- Nonachlor 1210 1230 1200 182 181 178 1213 1.3 180 1.2 1238 150 231 11 -0Dieldrin 238 241 236 58.3 59.5 58.4 238 1.1 58.7 1.1 209 56 37.5 3.9 0Mirex 47.3 48.6 47.2 37.1 36.8 35.3 47.7 1.6 36.4 2.6 43.7 7.5 18.9 2.8 0

Category z (2

≤ 2 1

2 to 3 

≥ 3 

z (2

Lipid (mass fraction (%)) 7.80 5.10 5.10 55.0 56.5 53.9 6.0 25.98 55.1 2.37 6.4 2.08 74.3 0.45 -0

*Certified values are in bold **See text for explaination

Homog. V SRM 1945 SRM 1945 Homog. V

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass)

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQA V AND SRM 1945

data are reported as if three figures are significant

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 7/11/2001 7/11/2001 7/11/2001 7/11/2001 7/11/2001 7/11/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

18 7.62 7.71 7.41 3.77 3.73 3.64 7.58 2.0 3.71 1.8 7.73 1.5 4.48 0.88 -028 2.90 <2.43 2.37 10.1 9.9 10.5 2.64 14.2 10.2 3.0 4.26 2.3 14.10 1.4 -131 <1.36 <1.83 <1.62 4.72 4.85 4.74 <1.60 <1.60 4.77 1.5 3.28 2.6 3.12 0.6944 38.1 38.1 38.0 12.6 12.3 12.3 38.1 0.2 12.4 1.4 35.3 3.8 12.2 1.4 049 98.4 99.9 97.3 18.5 18.8 18.2 98.5 1.3 18.5 1.6 86.1 11 20.8 2.8 052 153 156 152 41.0 43.0 41.6 154 1.4 41.9 2.5 143 8 43.6 2.5 066/95* see below see below see below see below see below see below 192 21 57.4 2.5 -487 170 169 166 26.7 25.9 26.2 168 1.2 26.3 1.5 157 20 16.7 1.4 099 430 433 431 63.1 62.7 62.0 431 0.4 62.6 0.9 467 34 45.4 5.4 -0101 (+90) 473 472 472 93.5 94.7 92.9 472 0.1 93.7 1.0 410 53 65.2 5.6 0105 164 165 161 28.9 28.8 28.4 163 1.3 28.7 0.9 161 19 30.1 2.3 0118 536 547 539 81.6 80.8 79.9 541 1.1 80.8 1.1 511 59 74.6 5.1 0128 186 190 186 24.1 24.3 23.2 187 1.2 23.9 2.5 178 25 23.7 1.7 0132 NA NA NA NA NA NA NA NA NA NA 160 68 29.1 6.0138 (+163+164) 1550 1580 1560 191 192 186 1563 1.0 190 1.7 1104 213 131.5 7.4 1149 333 336 334 88.6 88.6 87.3 334 0.5 88.2 0.9 320 43 107 8.4 0151 139 141 140 27.7 27.9 27.5 140 0.7 27.7 0.7 134 20 28.7 5.2 0153 2110 2150 2110 243 240 237 2123 1.1 240 1.3 1833 199 213.0 19 0156 6.78 6.84 6.06 11.9 11.6 11.0 6.6 6.6 11.5 4.0 18.5 11 10.3 1.1 -2170 (+190) 262 275 267 50.2 50.5 48.0 268 2.4 49.6 2.8 218 27 40.6 2.6 0180 939 989 964 151 151 146 964 2.6 149 1.9 798 87 107 5.3 0183 235 240 237 41.9 41.7 40.3 237 1.1 41.3 2.1 231 30 36.6 4.1 0187 489 497 488 128 129 125 491 1.0 127 1.6 436 62 105 9.1 0194 180 186 181 60.1 63.1 58.1 182 1.8 60.4 4.2 165 16 39.6 2.5 0195 42.0 43.4 41.5 10.5 10.6 10.0 42.3 2.3 10.4 3.1 40.0 9 17.7 4.3 0201 NA NA NA NA NA NA NA NA NA NA 87.7 52 17.0 0.89

206 91.8 95.5 92.5 49.0 49.7 46.5 93.3 2.1 48.4 3.5 83.1 7.8 31.1 2.7 0

209 60.2 62.4 60.1 15.8 16.4 15.0 60.9 2.1 15.7 4.5 69.4 7.2 10.6 1.1 -066 60.5 61.1 62.3 23.8 23.9 23.5 61.3 1.5 23.7 0.9 50.7 4 23.6 1.6 095 147 147 147 38.7 38.9 38.3 147 0.0 38.6 0.8 141 15 33.8 1.7 0

*Certified values are in bold **See text for explaination Category z (2

≤ 2 2

2 to 3 

≥ 3 

Homog. V SRM 1945 Homog. V SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass) 

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQAV AND SRM 1945

data are reported as if three figures are significant

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 10/9/2001 10/9/2001 10/9/2001 10/2/2001 10/2/2001 10/2/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

2,4’-DDT 172 175 169 78.2 79.9 86.4 172.1 1.9 81.5 5.3 83.1 22 106 14 4

4,4’-DDT 215 220 215 205 212 235 217 1.4 217 7.2 184 21 245 15 0

2,4’-DDE 153 152 147 37.0 37.8 40.6 151 2.3 38.5 4.8 110 28 12.3 0.87 14,4’-DDE 31900 31300 30700 510 498 513 31300 1.9 507 1.6 33401 6050 445 37 -02,4’-DDD 111 112 106 27.1 26.9 23.4 109.5 2.6 25.8 8.0 45.7 14 18.1 2.8 54,4’-DDD 1120 1130 1120 112 113 111 1123 0.5 112 1.1 1005 138 133 10 0HCB 1.12 1.25 1.51 33.3 31.7 36.4 1.29 16 33.8 7.1 1.89 1.4 32.9 1.7 -1α-HCH coelution coelution coelution coelution coelution coelution 6.43 1.0 16.2 3.4

β-HCH 327 320 338 23.0 17.4 2.85 328 2.7 14.41 72.2 409 42.5 8.00 1.4 -0γ-HCH 4.58 4.48 4.54 6.40 25.03 3.91 4.54 1.1 11.78 98.0 6.87 1.64 3.30 0.81 -1

Heptachlor Epoxide 98.7 98.5 95.9 14.9 15.7 13.4 97.7 1.6 14.7 7.8 99.8 18 10.8 1.3 -0Cis- Chlordane coelution coelution coelution coelution coelution coelution 37.4 5.4 46.9 2.8

Trans- Chlordane 17.3 16.8 16.5 17.8 17.8 22.3 16.9 2.6 19.3 13.3 11.5 8.2 12.9 3.5 1Oxychlordane 74.5 74.4 72.9 19.9 20.2 22.5 73.9 1.2 20.9 7.0 78.9 7.4 19.8 1.9 -0Cis- Nonachlor 38.9 39.8 38.7 13.2 13.4 14.6 39.1 1.5 13.7 5.7 98.4 17 48.7 7.6 -2Trans- Nonachlor 983 971 951 160 158 164 968 1.7 161 1.8 1238 150 231 11 -0Dieldrin 350 350 343 71.9 69.2 68.0 348 1.2 69.7 2.9 209 56 37.5 3.9 2Mirex 63.2 71.1 69.1 36.4 36.9 40.1 67.8 6.1 37.8 5.4 43.7 7.5 18.9 2.8 2

Category z (2

≤ 2 1

2 to 3 

≥ 3 

z (2

Lipid (mass fraction (%)) 0.67 0.64 0.66 65.1 66.7 68.6 0.7 2.73 66.8 2.66 6.4 2.08 74.3 0.45 -3

*Certified values are in bold **See text for explaination

Homog. V SRM 1945 SRM 1945 Homog. V

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass)

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQA V AND SRM 1945

data are reported as if three figures are significant

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 10/9/2001 10/9/2001 10/9/2001 10/2/2001 10/2/2001 10/2/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

18 8.59 9.45 9.80 24.0 23.6 25.0 9.28 6.7 24.21 2.8 7.73 1.5 4.48 0.88 028 3.33 3.50 3.23 10.4 12.5 11.1 3.35 4.1 11.4 9.5 4.26 2.3 14.10 1.4 -031 <LOD <LOD <LOD 5.93 7.58 3.57 <LOD <LOD 5.70 35 3.28 2.6 3.12 0.6944 41.2 44.6 42.2 11.0 10.0 18.2 42.6 4.1 13.1 34 35.3 3.8 12.2 1.4 049 106 106 103 20.6 21.1 24.5 105 2.0 22.1 9.7 86.1 11 20.8 2.8 052 152 145 141 32.8 34.9 40.3 146 3.8 36.0 11 143 8 43.6 2.5 066/95* see below see below see below see below see below see below 192 21 57.4 2.587 NA NA NA NA NA NA NA NA NA NA 157 20 16.7 1.4

99 coelution coelution coelution coelution coelution coelution 467 34 45.4 5.4

101 (+90) coelution coelution coelution coelution coelution coelution 410 53 65.2 5.6

105 192 222 186 26.4 26.7 29.5 200 9.8 27.5 6.3 161 19 30.1 2.3 1118 581 588 575 83.8 85.2 93.1 581 1.1 87.4 5.7 511 59 74.6 5.1 0128 259 239 233 132.1 133.2 142.6 243 5.6 136.0 4.2 178 25 23.7 1.7 1132 NA NA NA NA NA NA NA NA NA NA 160 68 29.1 6.0138 (+163+164) 1180 1190 1150 161 162 173 1173 1.8 166 4.1 1104 213 131.5 7.4 0149 388 394 376 87.7 88.7 94.6 386 2.4 90.3 4.1 320 43 107 8.4 0151 283 285 274 112 113 119 280 2.1 114.5 3.6 134 20 28.7 5.2 4153 1490 1490 1440 180 181 194 1473 2.0 185 4.1 1833 199 213.0 19 -0156 <LOD <LOD <LOD 12.0 11.8 13.2 <LOD <LOD 12.3 6.2 18.5 11 10.3 1.1

170 (+190) 262 264 259 42.8 37.8 40.5 262 1.0 40.3 6.2 218 27 40.6 2.6 0180 677 675 660 134 132 139 671 1.4 135 2.9 798 87 107 5.3 -0183 316 319 308 61.3 62.0 66.1 314 2.0 63.1 4.0 231 30 36.6 4.1 1187 458 463 445 132 134 142 455 2.1 136 3.9 436 62 105 9.1 0194 209 211 207 69.3 70.0 76.2 209 1.0 71.8 5.2 165 16 39.6 2.5 1195 47.3 48.5 44.9 9.0 8.4 10.2 46.9 3.9 9.19 10 40.0 9 17.7 4.3 0201 NA NA NA NA NA NA NA NA NA NA 87.7 52 17.0 0.89

206 87.6 94.1 92.0 40.2 40.3 44.0 91.2 3.6 41.5 5.2 83.1 7.8 31.1 2.7 0

209 86.4 88.1 87.1 23.7 24.7 27.9 87.2 1.0 25.4 8.6 69.4 7.2 10.6 1.1 166 54.7 53.3 50.4 19.1 19.5 24.3 52.8 4.1 21.0 14 50.7 4 23.6 1.6 095 193 196 190 43.3 46.1 48.1 193 1.6 45.9 5.3 141 15 33.8 1.7 1

*Certified values are in bold **See text for explaination Category z (2

≤ 2 2

2 to 3 

≥ 3 

Homog. V SRM 1945 Homog. V SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass) 

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQAV AND SRM 1945

data are reported as if three figures are significant

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 7/19/2001 7/19/2001 7/20/2001 7/19/2001 7/19/2001 7/19/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

2,4’-DDT 58.8 87.6 67.6 90.9 94.2 93.7 71.3 20.7 92.9 1.9 83.1 22 106 14 -04,4’-DDT 185 232 188 252 225 253 202 13.0 243 6.5 184 21 245 15 0

2,4’-DDE 113 114 113 8.87 6.53 6.50 113 0.5 7.30 19 110 28 12.3 0.87 04,4’-DDE 25600 26300 26100 425 458 435 26000 1.4 439 3.9 33401 6050 445 37 -02,4’-DDD 52.3 53.7 45.8 28.2 23.9 27.5 50.6 8.3 26.5 8.7 45.7 14 18.1 2.8 04,4’-DDD NA NA NA NA NA NA NA NA NA NA 1005 138 133 10HCB 0.150 0.150 0.120 21.4 24.7 23.3 0.140 12.4 23.1 7.2 1.89 1.4 32.9 1.7 -3α-HCH 5.51 5.73 5.41 13.1 13.0 14.1 5.55 2.9 13.4 4.5 6.43 1.0 16.2 3.4 -0

β-HCH 344 372 307 1.87 1.90 1.96 341 9.6 1.91 2.4 409 42.5 8.00 1.4 -0γ-HCH 6.29 5.30 6.43 2.33 2.38 2.57 6.01 10.3 2.43 5.2 6.87 1.64 3.30 0.81 -0

Heptachlor Epoxide 105 111 107 10.1 9.3 9.8 108 2.8 9.75 4.0 99.8 18 10.8 1.3 0Cis- Chlordane 39.4 44.8 49.0 46.2 42.4 46.8 44.4 10.8 45.1 5.3 37.4 5.4 46.9 2.8 0Trans- Chlordane 10.3 10.7 11.6 14.8 12.6 14.3 10.9 6.1 13.9 8.3 11.5 8.2 12.9 3.5 -0Oxychlordane 56.0 64.0 54.4 21.9 20.5 22.3 58.1 8.8 21.6 4.4 78.9 7.4 19.8 1.9 -1Cis- Nonachlor NA NA NA NA NA NA NA NA NA NA 98.4 17 48.7 7.6Trans- Nonachlor 1020 1090 915 163 166 170 1008 8.7 166 2.1 1238 150 231 11 -0Dieldrin 159 173 162 49.0 46.1 50.6 165 4.5 48.6 4.7 209 56 37.5 3.9 -0Mirex 41.5 41.2 40.0 29.1 34.6 30.9 40.9 1.9 31.5 8.9 43.7 7.5 18.9 2.8 -0

Category z (2

≤ 2 1

2 to 3 

≥ 3 

z (2

Lipid (mass fraction (%)) 6.14 6.11 6.13 73.5 81.2 72.2 6.1 0.25 75.6 6.44 6.4 2.08 74.3 0.45 -0

*Certified values are in bold **See text for explaination

Homog. V SRM 1945 SRM 1945 Homog. V

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass)

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQA V AND SRM 1945

data are reported as if three figures are significant

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 7/19/2001 7/19/2001 7/20/2001 7/19/2001 7/19/2001 7/19/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

18 10.60 9.56 9.50 3.81 4.47 4.11 9.89 6.3 4.13 8.0 7.73 1.5 4.48 0.88 128 NA NA NA NA NA NA NA NA NA NA 4.26 2.3 14.10 1.431 NA NA NA NA NA NA NA NA NA NA 3.28 2.6 3.12 0.6944 40.1 36.6 36.8 13.3 14.8 14.2 37.8 5.2 14.1 5.4 35.3 3.8 12.2 1.4 049 90.5 87.0 86.9 18.4 20.2 19.2 88.1 2.3 19.3 4.7 86.1 11 20.8 2.8 052 138 132 132 37.3 41.2 39.1 134 2.6 39.2 5.0 143 8 43.6 2.5 -066/95* see below see below see below see below see below see below 192 21 57.4 2.587 NA NA NA NA NA NA NA NA NA NA NA 20 16.7 1.4

99 409 403 400 58.9 66.2 62.5 404 1.1 62.5 5.8 467 34 45.4 5.4 -0101 (+90) 387 379 376 71.3 79.7 75.4 381 1.5 75.5 5.6 410 53 65.2 5.6 -0105 166 170 161 25.4 27.8 26.1 166 2.7 26.4 4.7 161 19 30.1 2.3 0118 538 540 534 78.2 88.4 83.7 537 0.6 83.4 6.1 511 59 74.6 5.1 0128 172 173 170 20.1 23.3 21.6 172 0.9 21.7 7.4 178 25 23.7 1.7 -0132 110 104 110 14.7 17.8 16.9 108.0 3 16.5 10 160 68 29.1 6.0 -1138 (+163+164) 1000 1010 1000 126 144 134 1003 0.6 135 6.7 1104 213 131.5 7.4 -0149 334 334 333 78.2 88.0 83.2 334 0.2 83.1 5.9 320 43 107 8.4 0151 136 139 134 23.7 26.4 24.2 136 1.8 24.8 5.8 134 20 28.7 5.2 0153 1540 1540 1529 180 203 191 1536 0.4 191 6.0 1833 199 213.0 19 -0156 <LOD <LOD <LOD 9.2 11.3 10.7 <LOD <LOD 10.4 10.7 18.5 11 10.3 1.1

170 (+190) 233 237 234 40.3 47.3 43.2 235 0.9 43.6 8.1 218 27 40.6 2.6 0180 726 735 731 126 144 133 731 0.6 134 6.8 798 87 107 5.3 -0183 216 220 217 34.1 39.1 36.3 218 1.0 36.5 6.9 231 30 36.6 4.1 -0187 NA NA NA NA NA NA NA NA NA NA 436 62 105 9.1

194 154 160 160 51.2 60.0 54.1 158 2.2 55.1 8.1 165 16 39.6 2.5 -0195 NA NA NA NA NA NA NA NA NA NA 40.0 9 17.7 4.3

201 42.6 38.0 41.0 22.2 28.0 24.0 40.5 5.8 24.7 12.0 87.7 52 17.0 0.89 -2206 74.5 85.3 86.9 44.6 51.3 47.1 82.2 8.2 47.7 7.1 83.1 7.8 31.1 2.7 0

209 48.8 65.2 69.6 19.8 20.1 20.0 61.2 17.9 20.0 0.8 69.4 7.2 10.6 1.1 -066 53.1 48.3 44.2 18.3 20.2 21.7 48.5 9.2 20.1 8.5 50.7 4 23.6 1.6 -095 143 142 148 41.2 46.3 40.3 144 2.2 42.6 7.6 141 15 33.8 1.7 0

*Certified values are in bold **See text for explaination Category z (2

≤ 2 2

2 to 3 

≥ 3 

Homog. V SRM 1945 Homog. V SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass) 

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQAV AND SRM 1945

data are reported as if three figures are significant

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements NA NA NA NA NA NA ng/g wet %RSD ng/g wet %RSD Value Value (25

2,4’-DDT NA NA NA NA NA NA NA NA NA NA 83.1 22 106 144,4’-DDT NA NA NA NA NA NA NA NA NA NA 184 21 245 152,4’-DDE NA NA NA NA NA NA NA NA NA NA 110 28 12.3 0.87

4,4’-DDE NA NA NA NA NA NA NA NA NA NA 33401 6050 445 372,4’-DDD NA NA NA NA NA NA NA NA NA NA 45.7 14 18.1 2.8

4,4’-DDD NA NA NA NA NA NA NA NA NA NA 1005 138 133 10HCB NA NA NA NA NA NA NA NA NA NA 1.89 1.4 32.9 1.7

α-HCH NA NA NA NA NA NA NA NA NA NA 6.43 1.0 16.2 3.4

β-HCH NA NA NA NA NA NA NA NA NA NA 409 42.5 8.00 1.4γ-HCH NA NA NA NA NA NA NA NA NA NA 6.87 1.64 3.30 0.81

Heptachlor Epoxide NA NA NA NA NA NA NA NA NA NA 99.8 18 10.8 1.3

Cis- Chlordane NA NA NA NA NA NA NA NA NA NA 37.4 5.4 46.9 2.8Trans- Chlordane NA NA NA NA NA NA NA NA NA NA 11.5 8.2 12.9 3.5

Oxychlordane NA NA NA NA NA NA NA NA NA NA 78.9 7.4 19.8 1.9

Cis- Nonachlor NA NA NA NA NA NA NA NA NA NA 98.4 17 48.7 7.6Trans- Nonachlor NA NA NA NA NA NA NA NA NA NA 1238 150 231 11Dieldrin NA NA NA NA NA NA NA NA NA NA 209 56 37.5 3.9Mirex NA NA NA NA NA NA NA NA NA NA 43.7 7.5 18.9 2.8

Category z (2

≤ 2 

2 to 3 

≥ 3 

z (2

Lipid (mass fraction (%)) 6.90 6.93 7.12 77.1 76.3 76.2 7.0 1.71 76.5 0.64 6.4 2.08 74.3 0.45 0

*Certified values are in bold **See text for explaination

Homog. V SRM 1945 SRM 1945 Homog. V

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass)

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQA V AND SRM 1945

data are reported as if three figures are significant

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 10/4/2001 10/4/2001 10/4/2001 10/4/2001 10/4/2001 10/4/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

18 4.37 4.92 5.01 1.88 1.81 1.74 4.77 7.3 1.81 3.9 7.73 1.5 4.48 0.88 -128 4.80 NA 5.06 15.7 15.1 14.9 4.93 3.7 15.2 2.7 4.26 2.3 14.10 1.4 031 2.09 NA 2.20 4.84 4.88 5.03 2.15 3.6 4.92 2 3.28 2.6 3.12 0.69 -144 43.7 45.3 45.4 13.0 13.6 12.5 44.8 2.1 13.0 4.2 35.3 3.8 12.2 1.4 149 106 108 108 18.2 18.3 17.3 107.3 1.1 17.9 3.1 86.1 11 20.8 2.8 152 NA NA NA NA NA NA NA NA NA NA 143 8 43.6 2.5

66/95* see below see below see below see below see below see below NA NA NA NA 192 21 57.4 2.587 NA NA NA NA NA NA NA NA NA NA 157 20 16.7 1.4

99 563 543 585 61.7 64.5 60.7 564 3.7 62.3 3.2 467 34 45.4 5.4 0101 (+90) 535 521 564 86.4 91.7 87.9 540 4.1 88.7 3.1 410 53 65.2 5.6 1105 187 191 189 26.6 27.5 28.4 189 1.1 27.5 3.3 161 19 30.1 2.3 0118 460 493 478 62.5 65.0 64.1 477 3.5 63.9 2.0 511 59 74.6 5.1 -0128 221 218 228 22.7 24.1 24.6 222 2.3 23.8 4.1 178 25 23.7 1.7 1132 NA NA NA NA NA NA NA NA NA NA 160 68 29.1 6.0138 (+163+164) NA NA NA NA NA NA NA NA NA NA 1104 213 131.5 7.4149 447 522 539 104 124 167 503 9.7 131.7 24.4 320 43 107 8.4 2151 170 195 214 31.7 35.9 52.9 193 11.4 40.2 27.9 134 20 28.7 5.2 1153 NA NA NA NA NA NA NA NA NA NA 1833 199 213.0 19156 7.00 7.00 6.93 10.2 10.8 11.0 7.0 0.6 10.7 3.9 18.5 11 10.3 1.1 -2170 (+190) 259 240 249 38.2 36.5 29.6 249 3.8 34.8 13.1 218 27 40.6 2.6 0180 994 1040 1050 160 164 164 1028 2.9 163 1.4 798 87 107 5.3 1183 257 288 295 40.1 44.1 48.8 280 7.2 44.3 9.8 231 30 36.6 4.1 0187 NA NA NA NA NA NA NA NA NA NA 436 62 105

194 210 211 216 63.4 64.5 65.5 212 1.5 64.5 1.6 165 16 39.6 2.5 1

195 37.8 44.0 45.1 9.11 10.4 12.6 42.3 9.3 10.7 16.5 40.0 9 17.7 4.3 0201 234.0 280.0 269.0 81.6 96.3 115.0 261.0 9.2 97.6 17.1 87.7 52 17.0 0.89 7206 108.0 111.0 108.0 51.4 60.1 57.6 109.0 1.6 56.4 7.9 83.1 7.8 31.1 2.7 1

209 78.8 79.1 80.0 19.9 20.8 20.5 79.3 0.8 20.4 2.2 69.4 7.2 10.6 1.1 066 38.2 NA 37.6 15.0 15.6 14.9 37.9 1.1 15.2 2.5 50.7 4 23.6 1.6 -195 155 141 167 34.2 35.4 36.2 154 8.4 35.3 2.9 141 15 33.8 1.7 0

*Certified values are in bold **See text for explaination Category z (2

≤ 2 2

2 to 3 

≥ 3 

Homog. V SRM 1945 Homog. V SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass) 

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Laboratory 

SAMPLES: MMQAV AND SRM 1945

data are reported as if three figures are significant

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-score 

Date(s) of measurements 11/10/2001 11/10/2001 11/10/2001 11/10/2001 11/10/2001 11/10/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25%) 

2,4’-DDT 160 170 170 120 120 130 166.7 3.5 123 4.7 83.1 22 106 14 4.0 

4,4’-DDT 96.0 91.0 100 220 240 230 95.7 4.7 230 4.3 184 21 245 15 -1.9 

2,4’-DDE 24.0 33.0 32.0 18.0 20.0 21.0 29.7 17 19.7 7.8 110 28 12.3 0.87 -2.9 

4,4’-DDE 18200 17200 20629 450 530 550 18676 9.4 510 10 33401 6050 445 37 -1.8 

2,4’-DDD 30.0 29.0 31.0 19.0 26.0 28.0 30.0 3.3 24.3 19 45.7 14 18.1 2.8 -1.4 

4,4’-DDD 500 490 460 100 100 110 483 4.3 103 5.6 1005 138 133 10 -2.1 

HCB <1 <1 <1 24.0 24.0 22.0 <1 <1 23.3 4.9 1.89 1.4 32.9 1.7

α-HCH 4.00 4.00 5.00 27.0 29.0 30.0 4.33 13 28.7 5.3 6.43 1.0 16.2 3.4 -1.3 

β-HCH NA NA NA NA NA NA NA NA NA NA 409 42.5 8.00 1.4

γ-HCH 3.00 3.00 3.00 19.0 20.0 20.0 3.00 0.0 19.7 2.9 6.87 1.64 3.30 0.81 -2.3 

Heptachlor Epoxide NA NA NA NA NA NA NA NA NA NA 99.8 18 10.8 1.3

Cis- Chlordane 22.0 24.0 25.0 49.0 53.0 53.0 23.7 6.5 51.7 4.5 37.4 5.4 46.9 2.8 -1.5 

Trans- Chlordane 5.0 4.0 4.0 22.0 24.0 26.0 4.33 13 24.0 8.3 11.5 8.2 12.9 3.5 -2.5 

Oxychlordane NA NA NA NA NA NA NA NA NA NA 78.9 7.4 19.8 1.9

Cis- Nonachlor NA NA NA NA NA NA NA NA NA NA 98.4 17 48.7 7.6

Trans- Nonachlor 640 650 680 130 150 160 657 3.2 147 10.4 1238 150 231 11 -1.9 

Dieldrin <1 <1 <1 92.0 150.0 440.0 <1 <1 227 82 209 56 37.5 3.9Mirex NA NA NA NA NA NA NA NA NA NA 43.7 7.5 18.9 2.8

Category z (25%) 

≤ 2 6 

2 to 3 4

≥ 3 1

z (25%) 

Lipid (mass fraction (%)) 3.40 NA NA 71.7 NA NA 3.4 71.7 6.4 2.08 74.3 0.45 -1.9 

*Certified values are in bold **See text for explaination

Homog. V SRM 1945 SRM 1945 Homog. V

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass)

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Laboratory 

SAMPLES: MMQA V AND SRM 1945

data are reported as if three figures are significant

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-score 

Date(s) of measurements 11/10/2001 11/10/2001 11/10/2001 11/10/2001 11/10/2001 11/10/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25%) 

18 55.0 53.0 55.0 3.00 3.00 3.00 54.3 2.1 3.00 0.0 7.73 1.5 4.48 0.88 24.1 

28 2.00 1.00 2.00 15.0 16.0 15.0 1.67 35 15.3 3.8 4.26 2.3 14.10 1.4 -2.4 31 <1 <1 <1 2 2 2 <1 <1 2.0 0 3.28 2.6 3.12 0.6944 19.0 16.0 18.0 <1 <1 <1 17.7 8.6 <1 <1 35.3 3.8 12.2 1.4 -2.0 

49 43.0 42.0 46.0 <1 2.0 1.0 43.7 4.8 1.50 47 86.1 11 20.8 2.8 -2.0 

52 67.0 58.0 64.0 18.0 20.0 18.0 63.0 7.3 18.7 6.2 143 8 43.6 2.5 -2.2 

66/95* <1 <1 <1 <1 <1 <1 <1 <1 <1 <1 192 21 57.4 2.5

87 NA NA NA NA NA NA NA NA NA NA 157 20 16.7 1.4

99 NA NA NA NA NA NA NA NA NA NA 467 34 45.4 5.4

101 (+90) 180 180 210 61.0 65.0 65.0 190 9.1 63.7 3.6 410 53 65.2 5.6 -2.1 

105 73 60 68 42.0 44.0 44.0 67.0 9.8 43.3 2.7 161 19 30.1 2.3 -2.3 

118 250 230 250 83.0 89.0 97.0 243 4.7 89.7 7.8 511 59 74.6 5.1 -2.1 

128 85.0 71.0 86.0 23.0 25.0 25.0 80.7 10 24.3 4.7 178 25 23.7 1.7 -2.2 

132 NA NA NA NA NA NA NA NA NA NA 160 68 29.1 6.0138 (+163+164) 490 520 590 160 160 170 533 9.6 163 3.5 1104 213 131.5 7.4 -2.1 

149 160 130 150 71.0 76.0 77.0 147 10 74.7 4.3 320 43 107 8.4 -2.2 151 60 58 59 23.0 25.0 26.0 59.0 1.7 24.7 6.2 134 20 28.7 5.2 -2.2 

153 870 770 880 210 210 220 840 7.2 213 2.7 1833 199 213.0 19 -2.2 

156 <1 <1 <1 19.0 19.0 20.0 <1 <1 19.3 3.0 18.5 11 10.3 1.1

170 (+190) 120 110 120 42.0 44.0 45.0 117 4.9 43.7 3.5 218 27 40.6 2.6 -1.9 

180 380 350 420 150 170 170 383 9.2 163 7.1 798 87 107 5.3 -2.1 183 110 97 110 46.0 47.0 48.0 106 7.1 47.0 2.1 231 30 36.6 4.1 -2.2 

187 220 210 240 110 120 130 223 6.8 120 8.3 436 62 105 9.1 -2.0 194 52.0 45.0 51.0 37.0 38.0 41.0 49.3 7.7 38.7 5.4 165 16 39.6 2.5 -2.8 

195 NA NA NA NA NA NA NA NA NA NA 40.0 9 17.7 4.3

201 NA NA NA NA NA NA NA NA NA NA 87.7 52 17.0 0.89

206 NA NA NA NA NA NA NA NA NA NA 83.1 7.8 31.1 2.7

209 NA NA NA NA NA NA NA NA NA NA 69.4 7.2 10.6 1.166 NA NA NA NA NA NA NA NA NA NA 50.7 4 23.6 1.695 NA NA NA NA NA NA NA NA NA NA 141 15 33.8 1.7

*Certified values are in bold **See text for explaination Category z (25%) 

≤ 2 4 

2 to 3 13 

≥ 3 1

Homog. V SRM 1945 Homog. V SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass) 

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQAV AND SRM 1945

data are reported as if three figures are significant

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 8/10/2001 8/10/2001 8/10/2001 8/10/2001 8/10/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

2,4’-DDT 65.7 64.8 NA 79.5 87.5 82.1 65.2 1.0 83.0 4.9 83.1 22 106 14 -0

4,4’-DDT 204 207 NA 245 271 245 206 1.0 254 5.9 184 21 245 15 0

2,4’-DDE 97.9 98.7 NA 12.5 12.7 11.8 98.3 0.6 12.3 3.8 110 28 12.3 0.87 -04,4’-DDE 33533 32256 NA 469 496 471 32895 2.7 479 3.2 33401 6050 445 37 -02,4’-DDD NA NA NA NA NA NA NA NA NA NA 45.7 14 18.1 2.8

4,4’-DDD 1108 1083 NA 107 124 105 1096 1.6 112 9.1 1005 138 133 10 0HCB 0.08 0.09 NA 25.8 25.3 23.3 0.09 8.3 24.8 5.3 1.89 1.4 32.9 1.7 -3α-HCH 4.07 3.80 NA 12.8 13.1 11.3 3.94 4.9 12.4 7.8 6.43 1.0 16.2 3.4 -1

β-HCH 315 335 NA 1.95 2.07 1.68 325 4.3 1.90 11 409 42.5 8.00 1.4 -0γ-HCH 6.10 6.13 NA 2.79 2.80 2.16 6.12 0.3 2.58 14 6.87 1.64 3.30 0.81 -0

Heptachlor Epoxide NA NA NA NA NA NA NA NA NA NA 99.8 18 10.8 1.3

Cis- Chlordane 32.6 33.5 NA 46.3 48.1 47.2 33.1 1.9 47.2 1.9 37.4 5.4 46.9 2.8 -0Trans- Chlordane 1.26 1.37 NA 11.0 11.5 11.2 1.32 5.9 11.2 2.0 11.5 8.2 12.9 3.5 -3Oxychlordane 77.6 70.6 NA 23.1 23.8 23.3 74.1 6.7 23.4 1.6 78.9 7.4 19.8 1.9 -0Cis- Nonachlor 230 232 NA 97.9 100 96.2 231 0.6 98.0 2.0 98.4 17 48.7 7.6 5Trans-Nonachlor 1201 1215 NA 164 176 158 1208 0.9 166 5.4 1238 150 231 11 -0Dieldrin NA NA NA NA NA NA NA NA NA NA 209 56 37.5 3.9Mirex NA NA NA NA NA NA NA NA NA NA 43.7 7.5 18.9 2.8

Category z (2

≤ 2 1

2 to 3 

≥ 3 

z (2

Lipid (mass fraction (%)) 7.22 6.46 _________ 72.9 74.3 73.5 6.8 7.86 73.6 0.99 6.4 2.08 74.3 0.45 0

*Certified values are in bold **See text for explaination

Homog. V SRM 1945 SRM 1945 Homog. V

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass)

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQA V AND SRM 1945

data are reported as if three figures are significant

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 8/10/2001 8/10/2001 8/10/2001 8/10/2001 8/10/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

18 NA NA NA NA NA NA NA NA NA NA 7.73 1.5 4.48 0.88

28 2.34 1.75 NA 8.6 8.4 8.0 2.05 20.4 8.3 3.4 4.26 2.3 14.10 1.4 -231 13.7 6.04 NA 5.83 5.78 5.04 9.9 55 5.6 8 3.28 2.6 3.12 0.69 844 36.0 35.9 NA 12.1 12.3 11.8 36.0 0.2 12.1 2.2 35.3 3.8 12.2 1.4 049 88.4 87.8 NA 16.8 17.2 16.0 88.1 0.5 16.7 3.8 86.1 11 20.8 2.8 052 132 135 NA 34.3 35.3 32.5 134 1.8 34.0 4.1 143 8 43.6 2.5 -066/95* NA NA NA NA NA NA NA NA NA NA 192 21 57.4 2.5

87 NA NA NA NA NA NA NA NA NA NA 157 20 16.7 1.4

99 469 467 NA 54.0 55.3 52.5 468 0.3 54.0 2.6 467 34 45.4 5.4 0101 (+90) 473 470 NA 79.7 82.5 77.0 471 0.4 79.7 3.4 410 53 65.2 5.6 0105 177 183 NA 27.7 30.6 27.9 180 2.7 28.8 5.6 161 19 30.1 2.3 0118 561 549 NA 78.2 84.9 77.3 555 1.6 80.1 5.2 511 59 74.6 5.1 0128 195 197 NA 22.6 24.3 21.8 196 0.8 22.9 5.5 178 25 23.7 1.7 0132 NA NA NA NA NA NA NA NA NA NA 160 68 29.1 6.0138 (+163+164) 1105 1095 NA 140 146 134 1100 0.6 140 4.2 1104 213 131.5 7.4 0149 326 330 NA 79.6 82.1 74.7 328 0.8 78.8 4.8 320 43 107 8.4 0151 139 142 NA 24.6 27.0 24.4 140 1.5 25.3 5.8 134 20 28.7 5.2 0153 1968 2002 NA 214 234 213 1985 1.2 220 5.4 1833 199 213.0 19 0156 33.6 29.1 NA 13.1 13.5 13.0 31.4 10.1 13.2 2.1 18.5 11 10.3 1.1 2170 (+190) 276 289 NA 42.9 51.3 44.5 283 3.3 46.2 9.6 218 27 40.6 2.6 1180 877 887 NA 150 162 152 882 0.8 155 4.3 798 87 107 5.3 0183 NA NA NA NA NA NA NA NA NA NA 231 30 36.6 4.1

187 657 667 NA 173 188 175 662 1.1 178 4.5 436 62 105 9.1 2194 172 184 NA 60.0 61.3 57.3 178 4.9 59.6 3.4 165 16 39.6 2.5 0195 NA NA NA NA NA NA NA NA NA NA 40.0 9 17.7 4.3

201 NA NA NA NA NA NA NA NA NA NA 87.7 52 17.0 0.89

206 NA NA NA NA NA NA NA NA NA NA 83.1 7.8 31.1 2.7

209 67.6 71.3 NA 20.4 19.4 17.9 69.5 3.7 19.2 6.8 69.4 7.2 10.6 1.1 066 NA NA NA NA NA NA NA NA NA NA 50.7 4 23.6 1.695 NA NA NA NA NA NA NA NA NA NA 141 15 33.8 1.7

*Certified values are in bold **See text for explaination Category z (2

≤ 2 1

2 to 3 

≥ 3 

Homog. V SRM 1945 Homog. V SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass) 

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQAV AND SRM 1945

data are reported as if three figures are significant

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 9/10/2001 9/10/2001 9/10/2001 9/10/2001 9/10/2001 9/10/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

2,4’-DDT 64.4 70.1 64.8 59.0 69.5 58.0 66.4 4.8 62.2 10 83.1 22 106 14 -04,4’-DDT 258 254 243 118 137 121 252 3.1 125 8.1 184 21 245 15 1

2,4’-DDE 158 164 158 22.5 22.4 11.9 160 2.2 18.9 32 110 28 12.3 0.87 14,4’-DDE 25500 25100 25500 426 456 443 25367 0.9 442 3.4 33401 6050 445 37 -12,4’-DDD 334 338 299 78.8 59.0 53.4 324 6.6 63.7 21 45.7 14 18.1 2.8 244,4’-DDD 1080 1080 1010 80.8 93.8 97.2 1057 3.8 90.6 9.6 1005 138 133 10 0HCB <2 2 2 25.2 30.5 22.3 2.36 3.6 26.0 16.0 1.89 1.4 32.9 1.7 1α-HCH 7.40 6.20 6.10 16.0 29.1 11.1 6.57 11 18.7 50 6.43 1.0 16.2 3.4 0

β-HCH 429 460 411 <2 <2 <1 433 5.7 <2 <2 409 42.5 8.00 1.4 0γ-HCH 7.60 8.20 8.30 10.80 7.80 4.38 8.03 4.7 7.66 41.9 6.87 1.64 3.30 0.81 0

Heptachlor Epoxide 52.9 89.7 53.4 <2 <2 4.0 65.3 32 4.00 99.8 18 10.8 1.3 -1Cis-Chlordane 31.5 37.4 33.7 38.1 39.3 39.8 34.2 8.7 39.1 2.2 37.4 5.4 46.9 2.8 -0Trans-Chlordane 5.43 4.48 7.48 7.5 8.9 10.1 5.80 26 8.83 15 11.5 8.2 12.9 3.5 -2Oxychlordane 91.9 71.5 68.1 13.1 14.5 13.9 77.2 17 13.8 5.1 78.9 7.4 19.8 1.9 -0Cis-Nonachlor 107 117 105 23.1 25.2 25.3 110 5.9 24.5 5.1 98.4 17 48.7 7.6 0Trans-Nonachlor 1190 1270 1120 138 145 131 1193 6.3 138 5.1 1238 150 231 11 -0Dieldrin 19.0 26.3 12.5 40.1 41.7 10.4 19.3 36 30.7 57 209 56 37.5 3.9 -3Mirex 41.7 46.9 35.4 27.1 29.8 30.6 41.3 14 29.2 6.3 43.7 7.5 18.9 2.8 -0

Category z (2

≤ 2 1

2 to 3 

≥ 3 

z (2

Lipid (mass fraction (%)) 6.62 9.46 10.31 75.7 70.6 82.0 8.8 21.97 76.1 7.50 6.4 2.08 74.3 0.45 1

*Certified values are in bold **See text for explaination

Homog. V SRM 1945 SRM 1945 Homog. V

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass)

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQA V AND SRM 1945

data are reported as if three figures are significant

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 9/10/2001 9/10/2001 9/10/2001 9/10/2001 9/10/2001 9/10/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

18 8.84 16.7 9.60 <2 <2 <2 11.7 37 <2 <2 7.73 1.5 4.48 0.88 228 36.2 30.0 57.3 46.7 47.2 11.6 41.2 35 35.2 58.0 4.26 2.3 14.10 1.4 3431 NA NA NA NA NA NA NA NA NA NA 3.28 2.6 3.12 0.6944 30.9 25.5 30.8 24.7 12.8 5.40 29.1 11 14.3 68.1 35.3 3.8 12.2 1.4 -049 NA NA NA NA NA NA NA NA NA NA 86.1 11 20.8 2.8

52 160 168 146 41.8 42.0 27.3 158 7.0 37.0 22.8 143 8.5 43.6 2.5 066/95* 108 107 106 22.2 23.4 21.0 107 0.9 22.2 5.4 192 21 57.4 2.5 -187 NA NA NA NA NA NA NA NA NA NA 157 20 16.7 1.4

99 NA NA NA NA NA NA NA NA NA NA 467 34 45.4 5.4

101 (+90) 599 588 545 115 122 108 577 4.9 115 6.1 410 53 65.2 5.6 1105 125 159 149 35.0 67.4 33.2 144 12 45.2 43 161 19 30.1 2.3 -0118 511 505 473 73.2 74.0 68.4 496 4.1 71.9 4.2 511 59 74.6 5.1 -0128 216 226 203 32.7 29.8 28.0 215 5.4 30.2 7.9 178 25 23.7 1.7 0132 NA NA NA NA NA NA NA NA NA NA 160 68 29.1 6.0138 (+163+164) 1390 1380 1302 131 140 109 1357 3.5 127 13 1104 213 131.5 7.4 0149 NA NA NA NA NA NA NA NA NA NA 320 43 107 8.4

151 NA NA NA NA NA NA NA NA NA NA 134 20 28.7 5.2

153 1910 1910 1782 166 177 171 1867 4.0 171 3.2 1833 199 213.0 19 0156 NA NA NA NA NA NA NA NA NA NA 18.5 11 10.3 1.1

170 (+190) 229 229 210 58.5 63.9 52.1 223 4.9 58.2 10 218 27 40.6 2.6 0180 792 788 731 101 110 91 770 4.4 101 9.5 798 87 107 5.3 -0183 NA NA NA NA NA NA NA NA NA NA 231 30 36.6 4.1

187 394 396 392 98.7 107 95.2 394 0.5 100 6.0 436 62 105 9.1 -0194 NA NA NA NA NA NA NA NA NA NA 165 16 39.6 2.5

195 29.7 30.3 31.1 2.80 0.802 2.40 30.4 2.3 2.00 52.8 40.0 9 17.7 4.3 -1201 NA NA NA NA NA 2.6 NA NA NA NA 87.7 52 17.0 0.89

206 70.6 69.4 65.2 30.0 31.2 23.6 68.4 4.1 28.3 14 83.1 7.8 31.1 2.7 -0

209 69.9 73.1 69.1 11.3 9.6 4.8 70.7 3.0 8.57 39 69.4 7.2 10.6 1.1 066 NA NA NA NA NA NA NA NA NA NA 50.7 4 23.6 1.695 NA NA NA NA NA NA NA NA NA NA 141 15 33.8 1.7

*Certified values are in bold **See text for explaination Category z (2

≤ 2 1

2 to 3 

≥ 3 

Homog. V SRM 1945 Homog. V SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass) 

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQAV AND SRM 1945

data are reported as if three figures are significant

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 8/19-22/01 8/19-22/01 8/19-22/01 8/19-22/01 8/19-22/01 8/19-22/01 ng/g wet %RSD ng/g wet %RSD Value Value (25

2,4’-DDT NA NA NA NA NA NA NA NA NA NA 83.1 22 106 144,4’-DDT 200 205 214 255 251 321 206 3.4 276 14 184 21 245 15 0

2,4’-DDE NA NA NA NA NA NA NA NA NA NA 110 28 12.3 0.87

4,4’-DDE 46600 50400 54400 722 711 710 50467 7.7 714 0.9 33401 6050 445 37 22,4’-DDD NA NA NA NA NA NA NA NA NA NA 45.7 14 18.1 2.8

4,4’-DDD 930 924 1000 127 132 131 951 4.4 130 2.0 1005 138 133 10 -0

HCB <5 <5 <5 28.2 28.2 28.7 <5 <5 28.4 1.0 1.89 1.4 32.9 1.7α-HCH 7.29 7.79 8.16 14.7 15.1 15.5 7.75 5.6 15.1 2.6 6.43 1.0 16.2 3.4 0β-HCH 322 399 388 1.31 1.42 1.37 370 11 1.37 4.0 409 42.5 8.00 1.4 -0γ-HCH 6.81 6.98 7.20 2.64 2.62 2.65 7.00 2.8 2.64 0.6 6.87 1.64 3.30 0.81 0

Heptachlor Epoxide 108 95.9 102 12.3 11.8 11.6 102 5.9 11.9 3.0 99.8 18 10.8 1.3 0Cis-Chlordane 34.3 36.3 37.0 52.5 54.7 56.1 35.9 3.9 54.4 3.3 37.4 5.4 46.9 2.8 -0Trans-Chlordane 9.9 8.15 10.8 14.0 13.2 14.3 9.60 14 13.8 4.1 11.5 8.2 12.9 3.5 -0Oxychlordane 73.5 77.3 83.1 18.2 18.9 19.3 78.0 6.2 18.8 3.0 78.9 7.4 19.8 1.9 0Cis-Nonachlor 122 108 129 51.4 60.0 53.9 120 8.9 55.1 8.0 98.4 17 48.7 7.6 0Trans-Nonachlor 1240 1330 1590 222 223 224 1387 13 223 0.4 1238 150 231 11 0Dieldrin 201 258 281 59.7 61.0 57.8 247 17 59.5 2.7 209 56 37.5 3.9 0Mirex NA NA NA NA NA NA NA NA NA NA 43.7 7.5 18.9 2.8

Category z (2

≤ 2 1

2 to 3 

≥ 3 

z (2

Lipid (mass fraction (%)) 6.37 6.59 6.80 72.5 73.0 73.6 6.6 3.26 73.0 0.75 6.4 2.08 74.3 0.45 0

*Certified values are in bold **See text for explaination

Homog. V SRM 1945 SRM 1945 Homog. V

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass)

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQA V AND SRM 1945

data are reported as if three figures are significant

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 8/19-22/01 8/19-22/01 8/19-22/01 8/19-22/01 8/19-22/01 8/19-22/01 ng/g wet %RSD ng/g wet %RSD Value Value (25

18 NA NA NA NA NA NA NA NA NA NA 7.73 1.5 4.48 0.88

28 coelution coelution coelution coelution coelution coelution 4.26 2.3 14.10 1.431 coelution coelution coelution coelution coelution coelution 3.28 2.6 3.12 0.6944 27.3 28.4 29.4 9.36 9.04 9.55 28.4 3.7 9.3 2.8 35.3 3.8 12.2 1.4 -049 81.3 84.0 87.6 15.6 15.0 16.1 84.3 3.7 15.6 3.5 86.1 11 20.8 2.8 -052 107 111 116 29.2 28.2 30.0 111 4.1 29.1 3.1 143 8 43.6 2.5 -066/95* see below see below see below see below see below see below 192 21 57.4 2.587 103 110 109 15.2 14.8 15.4 107 3.5 15.1 2.0 157 20 16.7 1.4 -199 392 414 420 51.3 50.1 52.7 409 3.6 51.4 2.5 467 34 45.4 5.4 -0101 (+90) 266 282 285 50.4 49.7 51.4 278 3.7 50.5 1.7 410 53 65.2 5.6 -1105 170 181 179 27.7 27.2 28.5 177 3.3 27.8 2.4 161 19 30.1 2.3 0118 455 484 482 70.9 70.0 73.0 474 3.4 71.3 2.2 511 59 74.6 5.1 -0128 94.2 99.4 100 14.1 13.6 14.3 97.7 3.1 14.0 2.6 178 25 23.7 1.7 -1132 218 230 228 30.1 29.4 30.8 225.3 2.9 30.1 2.3 160 68 29.1 6.0 1138 (+163+164) 1250 1330 1320 158 155 161 1300 3.4 158 1.9 1104 213 131.5 7.4 0149 220 233 235 53.9 53.0 55.1 229 3.6 54.0 2.0 320 43 107 8.4 -1151 76.7 81.5 81.2 15.5 15.2 15.9 79.8 3.4 15.5 2.3 134 20 28.7 5.2 -1153 1730 1850 1960 201 197 202 1847 6.2 200 1.3 1833 199 213.0 19 0156 7.58 7.95 7.74 10.5 10.4 10.9 7.76 2.4 10.6 2.5 18.5 11 10.3 1.1 -2170 (+190) 189 200 196 31.2 30.2 31.3 195 2.9 30.9 2.0 218 27 40.6 2.6 -0180 823 870 865 147 143 148 853 3.0 146 1.8 798 87 107 5.3 0183 213 224 224 35.9 35.0 37.3 220 2.9 36.1 3.2 231 30 36.6 4.1 -0187 367 387 385 104 102 105 380 2.9 104 1.5 436 62 105 9.1 -0194 116 122 121 43.2 42.0 43.1 120 2.7 42.8 1.6 165 16 39.6 2.5 -1195 20.9 22.0 21.8 5.71 5.51 5.86 21.6 2.7 5.69 3.1 40.0 9 17.7 4.3 -1201 coelution coelution coelution coelution coelution coelution 87.7 52 17.0 0.89 -4

206 NA NA NA NA NA NA NA NA NA NA 83.1 7.8 31.1 2.7

209 NA NA NA NA NA NA NA NA NA NA 69.4 7.2 10.6 1.1

66 50.4 53.1 53.6 22.0 21.4 21.4 52.4 3.3 21.6 1.6 50.7 4 23.6 1.6 095 85.9 91.0 92.7 22.4 21.7 22.8 89.9 3.9 22.3 2.5 141 15 33.8 1.7 -1

*Certified values are in bold **See text for explaination Category z (2

≤ 2 2

2 to 3 

≥ 3 

Homog. V SRM 1945 Homog. V SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass) 

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQAV AND SRM 1945

data are reported as if three figures are significant

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 6/3/2001 6/3/2001 6/3/2001 6/3/2001 6/3/2001 6/3/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

2,4’-DDT 65.6 58.7 62.3 93.6 98.7 93.6 62.2 5.5 95.3 3.1 83.1 22 106 14 -14,4’-DDT 155 139 149 237 239 237 148 5.7 237 0.5 184 21 245 15 -0

2,4’-DDE 127 115 121 17.4 22.0 18.8 121.0 4.8 19.4 12 110 28 12.3 0.87 04,4’-DDE 31714 18813 29436 563 507 563 26654 25.8 545 5.9 33401 6050 445 37 -02,4’-DDD 73.0 63.9 68.2 19.0 21.7 19.0 68.4 6.7 19.9 7.9 45.7 14 18.1 2.8 24,4’-DDD 887 550 832 106 104 106 756 23.9 105 1.4 1005 138 133 10 -1

HCB 3.07 2.93 2.89 30.5 28.3 30.5 2.96 3.2 29.8 4.1 1.89 1.4 32.9 1.7 2α-HCH 9.20 8.96 9.55 12.1 12.2 12.1 9.24 3.2 12.1 0.6 6.43 1.0 16.2 3.4 1

β-HCH 455 414 416 4.86 4.44 4.86 428 5.4 4.72 5.1 409 42.5 8.00 1.4 0γ-HCH 12.57 10.38 11.35 7.60 8.26 7.60 11.4 9.6 7.82 4.8 6.87 1.64 3.30 0.81 2

Heptachlor Epoxide 109 92 101 13.1 13.1 13.1 101 8.4 13.1 0.3 99.8 18 10.8 1.3 0Cis-Chlordane 32.2 30.9 34.1 61.6 63.3 57.1 32.4 5.0 60.7 5.3 37.4 5.4 46.9 2.8 -0Trans-Chlordane <LOD <LOD <LOD 12.5 13.1 12.5 <LOD <LOD 12.7 2.7 11.5 8.2 12.9 3.5Oxychlordane 94.6 84.0 88.3 24.2 25.3 24.2 89.0 6.0 24.5 2.6 78.9 7.4 19.8 1.9 0Cis-Nonachlor 59.8 53.2 58.3 50.6 50.1 50.6 57.1 6.1 50.4 0.5 98.4 17 48.7 7.6 -1Trans-Nonachlor 1210 753 1126 181 172 181 1030 23.6 178 2.7 1238 150 231 11 -0Dieldrin 277 249 256 54.9 56.5 54.9 261 5.7 55.5 1.7 209 56 37.5 3.9 1Mirex 56.6 51.9 53.9 33.5 37.7 33.5 54.1 4.3 34.9 7.0 43.7 7.5 18.9 2.8 0

Category z (2

≤ 2 1

2 to 3 

≥ 3 

z (2

Lipid (mass fraction (%)) 5.37 4.85 4.58 71.6 67.5 62.9 4.9 8.14 67.3 6.46 6.4 2.08 74.3 0.45 -0

*Certified values are in bold **See text for explaination

Homog. V SRM 1945 SRM 1945 Homog. V

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass)

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQA V AND SRM 1945

data are reported as if three figures are significant

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 6/3/2001 6/3/2001 6/3/2001 6/3/2001 6/3/2001 6/3/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

18 <LOD <LOD <LOD 1.53 1.53 1.53 <LOD <LOD 1.53 0.2 7.73 1.5 4.48 0.88

28 15.30 15.82 15.54 13.8 14.5 13.8 15.55 1.7 14.0 3.2 4.26 2.3 14.10 1.4 1031 2.99 2.77 2.91 2.97 3.29 2.97 2.89 3.9 3.07 6.1 3.28 2.6 3.12 0.69 -044 50.2 47.3 51.1 14.1 12.1 14.1 49.5 4.0 13.4 8.6 35.3 3.8 12.2 1.4 149 111.1 100.1 104.8 17.2 15.5 17.2 105 5.3 16.6 5.8 86.1 11 20.8 2.8 052 175 157 163 36.4 39.5 36.4 165 5.5 37.4 4.7 143 8 43.6 2.5 066/95* see below see below see below see below see below see below 192 21 57.4 2.5 -487 176 162 172 22.4 26.6 22.4 170 4.2 23.8 10 157 20 16.7 1.4 099 628 578 592 69.2 76.0 57.1 599 4.3 67.4 14 467 34 45.4 5.4 1101 (+90) 503 482 445 85.0 96.3 83.5 476 6.2 88.2 7.9 410 53 65.2 5.6 0105 197 193 199 28.5 30.7 28.5 196 1.7 29.2 4.4 161 19 30.1 2.3 0118 665 592 629 77.4 83.9 77.4 629 5.8 79.6 4.7 511 59 74.6 5.1 0128 242 219 231 40.0 40.9 38.8 231 4.9 39.9 2.7 178 25 23.7 1.7 1132 NA NA NA NA NA NA NA NA NA NA 160 68 29.1 6.0138 (+163+164) 1504 946 1418 202 227 198 1289 23.3 209 7.5 1104 213 131.5 7.4 0149 285 261 267 72.4 82.7 72.4 271 4.6 75.9 7.8 320 43 107 8.4 -0151 149 131 132 30.5 33.2 30.5 137 7.4 31.4 5.0 134 20 28.7 5.2 0153 2160 1335 2057 261 296 261 1851 24 273 7.6 1833 199 213.0 19 0156 21.4 18.2 19.8 16.2 18.8 16.2 19.8 7.9 17.1 8.9 18.5 11 10.3 1.1 0170 (+190) 299 270 287 47.3 50.7 47.3 285 5.2 48.4 4.2 218 27 40.6 2.6 1180 1907 1216 1825 175 171 164 1649 23 170 3.2 798 87 107 5.3 4

183 345 312 329 109.4 110.4 109.4 329 5.0 110 0.5 231 30 36.6 4.1 1187 568 509 543 131 147 131 540 5.5 136 6.8 436 62 105 9.1 1194 217 197 212 53.3 60.8 53.3 209 4.9 55.8 7.8 165 16 39.6 2.5 1195 115.6 99.9 108.5 27.6 35.8 28.1 108.0 7.3 30.5 15 40.0 9 17.7 4.3 6201 53.3 44.7 53.0 15.8 16.3 15.8 50.4 9.7 16.0 2.0 87.7 52 17.0 0.89 -1206 106.1 95.9 103.1 39.0 47.0 39.0 101.7 5.1 41.6 11 83.1 7.8 31.1 2.7 0

209 87.3 77.8 83.6 15.9 19.7 15.6 82.9 5.8 17.0 13 69.4 7.2 10.6 1.1 066 45.2 40.6 41.7 18.2 20.2 18.2 42.5 5.6 18.8 6.1 50.7 4 23.6 1.6 -095 169 151 158 46.9 40.6 40.9 159 5.5 42.8 8.2 141 15 33.8 1.7 0

*Certified values are in bold **See text for explaination Category z (2

≤ 2 2

2 to 3 

≥ 3 

Homog. V SRM 1945 Homog. V SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass) 

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Laboratory 

SAMPLES: MMQAV AND SRM 1945

data are reported as if three figures are significant

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-score 

Date(s) of measurements 15, Sept. 2001 15, Sept. 2001 15, Sept. 2001 15, Sept. 2001 15, Sept. 2001 15, Sept. 2001 ng/g wet %RSD ng/g wet %RSD Value Value (25%) 

2,4’-DDT NA NA NA NA NA NA NA NA NA NA 83.1 22 106 14

4,4’-DDT 198 197 216 307 309 316 204 5.2 311 1.6 184 21 245 15 0.4 

2,4’-DDE NA NA NA NA NA NA NA NA NA NA 110 28 12.3 0.87

4,4’-DDE 60411 47995 49917 519 642 634 52774 12.7 598 11.5 33401 6050 445 37 2.3 

2,4’-DDD NA NA NA NA NA NA NA NA NA NA 45.7 14 18.1 2.8

4,4’-DDD 1095 1116 1220 118 121 139 1144 5.9 126 8.8 1005 138 133 10 0.6 

HCB ND ND ND 22.7 24.7 27.3 ND ND 24.9 9.2 1.89 1.4 32.9 1.7

α-HCH 4.96 5.51 5.50 10.4 11.4 11.6 5.32 6.0 11.1 5.5 6.43 1.0 16.2 3.4 -0.7 

β-HCH 369 380 398 <LOD <LOD <LOD 382 3.8 <LOD <LOD 409 42.5 8.00 1.4 -0.3 

γ-HCH 4.32 4.79 4.96 1.64 1.69 1.89 4.69 7.0 1.74 7.4 6.87 1.64 3.30 0.81 -1.3 

Heptachlor Epoxide NA NA NA NA NA NA NA NA NA NA 99.8 18 10.8 1.3

Cis-Chlordane 28.8 28.1 40.9 46.8 45.9 48.7 32.6 22.0 47.1 3.0 37.4 5.4 46.9 2.8 -0.5 

Trans-Chlordane <LOD <LOD <LOD 10.8 13.2 9.2 <LOD <LOD 11.1 18.2 11.5 8.2 12.9 3.5Oxychlordane 102.1 99.8 110.0 20.6 21.2 22.5 103.9 5.1 21.4 4.5 78.9 7.4 19.8 1.9 1.3 

Cis-Nonachlor 112.5 115.2 126.7 48.4 46.6 48.0 118.1 6.4 47.7 2.0 98.4 17 48.7 7.6 0.8 

Trans-Nonachlor 1773 1608 1854 176 194 171 1745 7.2 180 6.8 1238 150 231 11 1.6 

Dieldrin NA NA NA NA NA NA NA NA NA NA 209 56 37.5 3.9Mirex NA NA NA NA NA NA NA NA NA NA 43.7 7.5 18.9 2.8

Category z (25%) 

≤ 2 9 

2 to 3 1

≥ 3 0

z (25%) 

Lipid (mass fraction (%)) 7.44 7.44 7.44 69.0 69.0 69.0 7.4 0.00 69.0 0.00 6.4 2.08 74.3 0.45 0.6 

*Certified values are in bold **See text for explaination

Data as Submitted by Laboratory (ng/g wet mass)

Homog V SRM 1945

Homog. V SRM 1945 SRM 1945 Homog. V

Values for Materials (ng/g wet mass)
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Laboratory 

SAMPLES: MMQA V AND SRM 1945

data are reported as if three figures are significant

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-score 

Date(s) of measurements 15, Sept. 2001 15, Sept. 2001 15, Sept. 2001 15, Sept. 2001 15, Sept. 2001 15, Sept. 2001 ng/g wet %RSD ng/g wet %RSD Value Value (25%) 

18 <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD <LOD 7.73 1.5 4.48 0.88

28 <LOD <LOD <LOD 13.0 17.6 12.0 <LOD <LOD 14.2 21.2 4.26 2.3 14.10 1.431 NA NA NA NA NA NA NA NA NA NA 3.28 2.6 3.12 0.6944 22.5 32.0 32.0 5.5 8.9 8.7 28.8 19.0 7.7 25.0 35.3 3.8 12.2 1.4 -0.7 

49 92.5 92.3 94.6 15.2 14.6 14.4 93.1 1.3 14.7 2.6 86.1 11 20.8 2.8 0.3 

52 143 143 146 32.8 31.7 31.4 144 1.1 32.0 2.2 143 8 43.6 2.5 0.0 

66/95* see below see below see below see below see below see below 192 21 57.4 2.5

87 137 148 148 17.7 16.9 18.1 144 4.6 17.6 3.5 157 20 16.7 1.4 -0.3 

99 466 458 470 51.1 48.1 48.2 465 1.3 49.1 3.4 467 34 45.4 5.4 0.0 

101 (+90) 444 438 448 70.6 66.4 67.2 443 1.2 68.1 3.3 410 53 65.2 5.6 0.3 

105 53 191 137 5.3 24.6 24.4 127 54.9 18.1 61.4 161 19 30.1 2.3 -0.8 

118 647 639 658 82.1 79.2 78.8 648 1.5 80.0 2.3 511 59 74.6 5.1 1.1 

128 262 260 266 6.4 12.1 12.2 262 1.2 10.3 32.4 178 25 23.7 1.7 1.9 

132 262 260 266 24.4 29.1 29.3 262.3 1 27.6 10 160 68 29.1 6.0 2.6 138 (+163+164) 1174 1202 1232 101 126 127 1203 2.4 118 12.8 1104 213 131.5 7.4 0.4 

149 291 281 296 48.6 57.1 57.9 290 2.7 54.6 9.4 320 43 107 8.4 -0.4 151 108 108 111 14.4 17.2 17.3 109 1.3 16.3 10.2 134 20 28.7 5.2 -0.8 

153 1801 1781 1832 144 171 172 1805 1.4 162 9.9 1833 199 213.0 19 -0.1 

156 66.9 67.1 67.9 17.6 19.5 19.6 67.3 0.7 18.9 5.8 18.5 11 10.3 1.1 10.5 

170 (+190) 165 166 169 23.0 22.5 22.5 167 1.2 22.7 1.4 218 27 40.6 2.6 -0.9 

180 693 689 705 104 100 100 696 1.1 101 2.0 798 87 107 5.3 -0.5 183 14 14 14 NA NA NA 14 1.5 NA NA 231 30 36.6 4.1 -3.8 

187 202 201 207 29 27 28 204 1.7 28 2.7 436 62 105 9.1 -2.1 194 123 123 132 32.8 33.0 33.6 126 4.4 33.1 1.2 165 16 39.6 2.5 -0.9 

195 26.1 26.3 27.1 4.8 5.2 5.5 26.5 1.9 5.2 6.2 40.0 9 17.7 4.3 -1.3 

201 176.2 175.2 179.7 52.6 51.7 52.4 177.0 1.4 52.2 0.9 87.7 52 17.0 0.89 4.1 

206 70.9 71.3 71.4 34.8 41.1 38.3 71.2 0.4 38.1 8.3 83.1 7.8 31.1 2.7 -0.6 

209 74.6 75.8 75.6 19.5 22.6 21.3 75.4 0.8 21.1 7.5 69.4 7.2 10.6 1.1 0.3 66 82.8 83.2 85.5 26.6 25.6 23.3 83.8 1.8 25.2 6.6 50.7 4 23.6 1.6 2.6 95 128 129 130 27.0 25.7 26.7 129 0.9 26.5 2.5 141 15 33.8 1.7 -0.4 

*Certified values are in bold **See text for explaination Category z (25%) 

≤ 2 20 

2 to 3 3

≥ 3 3

Homog V SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass) 

Homog. V SRM 1945 Homog. V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQAV AND SRM 1945

data are reported as if three figures are significant

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 07/26/01 07/26/01 07/26/01 07/26/01 07/26/01 07/26/01 ng/g wet %RSD ng/g wet %RSD Value Value (25

2,4’-DDT 63.0 65.0 72.0 72.0 75.0 58.0 66.7 7.1 68.3 13 83.1 22 106 14 -04,4’-DDT 150 110 150 180 190 150 137 17 173 12 184 21 245 15 -1

2,4’-DDE 81.0 72.0 83.0 11.0 11.0 11.0 78.7 7.4 11.0 0.0 110 28 12.3 0.87 -14,4’-DDE 40000 34000 39000 530 560 480 37667 8.5 523 7.7 33401 6050 445 37 02,4’-DDD 35.0 35.0 38.0 19.0 17.0 16.0 36.0 4.8 17.3 8.8 45.7 14 18.1 2.8 -04,4’-DDD 778 610 730 80.0 89.0 79.0 706 12 82.7 6.7 1005 138 133 10 -1

HCB <1.1 <1.0 <0.77 32.0 34.0 32.0 <1 <1 32.7 3.5 1.89 1.4 32.9 1.7

α-HCH 5.60 5.90 6.60 16.0 15.0 14.0 6.03 8.5 15.0 6.7 6.43 1.0 16.2 3.4 -0

β-HCH 380 320 380 2.00 2.30 1.50 360 9.6 1.93 21 409 42.5 8.00 1.4 -0γ-HCH 36.00 20.00 35.00 7.60 15.0 5.90 30.33 30 9.50 51 6.87 1.64 3.30 0.81 13

Heptachlor Epoxide NA NA NA NA NA NA NA NA NA NA 99.8 18 10.8 1.3

Cis- Chlordane 45.0 45.0 46.0 53.0 55.0 51.0 45.3 1.3 53.0 3.8 37.4 5.4 46.9 2.8 0Trans- Chlordane 2.90 2.60 2.80 11.0 11.0 11.0 2.77 5.5 11.0 0.0 11.5 8.2 12.9 3.5 -3Oxychlordane NA NA NA NA NA NA NA NA NA NA 78.9 7.4 19.8 1.9

Cis- Nonachlor NA NA NA NA NA NA NA NA NA NA 98.4 17 48.7 7.6Trans- Nonachlor NA NA NA NA NA NA NA NA NA NA 1238 150 231 11Dieldrin NA NA NA NA NA NA NA NA NA NA 209 56 37.5 3.9Mirex NA NA NA NA NA NA NA NA NA NA 43.7 7.5 18.9 2.8

Category z (2

≤ 2 

2 to 3 

≥ 3 

z (2

Lipid (mass fraction (%)) 22.00 21.00 22.00 74.0 75.0 74.0 21.7 2.66 74.3 0.78 6.4 2.08 74.3 0.45 9

*Certified values are in bold **See text for explaination

Homog. V SRM 1945 SRM 1945 Homog. V

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass)

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQA V AND SRM 1945

data are reported as if three figures are significant

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 07/26/01 07/26/01 07/26/01 07/26/01 07/26/01 07/26/01 ng/g wet %RSD ng/g wet %RSD Value Value (25

18 7.90 6.70 7.60 2.90 2.80 3.00 7.40 8.4 2.90 3.4 7.73 1.5 4.48 0.88 -028 2.30 2.20 1.70 11.0 12.0 9.4 2.07 16 10.8 12 4.26 2.3 14.10 1.4 -231 <1.1 <1.0 <0.77 4.40 4.10 4.60 <1 <1 4.37 6 3.28 2.6 3.12 0.6944 41.0 37.0 41.0 13.0 14.0 13.0 39.7 5.8 13.3 4.3 35.3 3.8 12.2 1.4 049 100.0 93.0 100.0 19.0 20.0 20.0 97.7 4.1 19.7 2.9 86.1 11 20.8 2.8 052 160 140 160 40.0 44.0 43.0 153 7.5 42.3 4.9 143 8 43.6 2.5 066/95* see below see below see below see below see below see below 192 21 57.4 2.587 190 170 180 28.0 32.0 29.0 180 5.6 29.7 7.0 157 20 16.7 1.4 099 490 430 490 52.0 57.0 53.0 470 7.4 54.0 4.9 467 34 45.4 5.4 0101 (+90) 520 450 510 78.0 89.0 78.0 493 7.7 81.7 7.8 410 53 65.2 5.6 0105 180 150 170 28.0 35.0 30.0 167 9.2 31.0 12 161 19 30.1 2.3 0118 680 530 670 78.0 88.0 73.0 627 13 79.7 9.6 511 59 74.6 5.1 0128 NA NA NA NA NA NA NA NA NA NA 178 25 23.7 1.7

132 NA NA NA NA NA NA NA NA NA NA 160 68 29.1 6.0138 (+163+164) 1600 1400 1600 180 190 170 1533 7.5 180 5.6 1104 213 131.5 7.4 1149 400 350 390 88.0 90.0 77.0 380 7.0 85.0 8.2 320 43 107 8.4 0151 170 150 160 30.0 34.0 30.0 160 6.3 31.3 7.4 134 20 28.7 5.2 0153 2300 2100 2300 220 240 210 2233 5.2 223 6.8 1833 199 213.0 19 0156 33.0 28.0 32.0 14.0 16.0 16.0 31.0 8.5 15.3 7.5 18.5 11 10.3 1.1 2170 (+190) 270 240 270 47.0 50.0 43.0 260 6.7 46.7 7.5 218 27 40.6 2.6 0180 910 820 900 140 150 140 877 5.6 143 4.0 798 87 107 5.3 0183 270 240 260 41.0 44.0 41.0 257 6.0 42.0 4.1 231 30 36.6 4.1 0187 490 420 480 120 120 110 463 8.2 117 4.9 436 62 105 9.1 0194 170 140 160 56.0 52.0 51.0 157 9.8 53.0 5.0 165 16 39.6 2.5 -0195 NA NA NA NA NA NA NA NA NA NA 40.0 9 17.7 4.3

201 NA NA NA NA NA NA NA NA NA NA 87.7 52 17.0 0.89

206 NA NA NA NA NA NA NA NA NA NA 83.1 7.8 31.1 2.7

209 NA NA NA NA NA NA NA NA NA NA 69.4 7.2 10.6 1.1

66 NA NA NA NA NA NA NA NA NA NA 50.7 4 23.6 1.695 180 150 170 39.0 46.0 41.0 167 9.2 42.0 8.6 141 15 33.8 1.7 0

*Certified values are in bold **See text for explaination Category z (2

≤ 2 1

2 to 3 

≥ 3 

Homog. V SRM 1945 Homog. V SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass) 

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQAV AND SRM 1945

data are reported as if three figures are significant

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 7/1/2001 7/1/2001 7/1/2001 7/1/2001 7/1/2001 7/1/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

2,4’-DDT NA NA NA NA NA NA NA NA NA NA 83.1 22 106 144,4’-DDT NA NA NA NA NA NA NA NA NA NA 184 21 245 152,4’-DDE NA NA NA NA NA NA NA NA NA NA 110 28 12.3 0.87

4,4’-DDE NA NA NA NA NA NA NA NA NA NA 33401 6050 445 372,4’-DDD NA NA NA NA NA NA NA NA NA NA 45.7 14 18.1 2.8

4,4’-DDD NA NA NA NA NA NA NA NA NA NA 1005 138 133 10HCB NA NA NA NA NA NA NA NA NA NA 1.89 1.4 32.9 1.7

α-HCH NA NA NA NA NA NA NA NA NA NA 6.43 1.0 16.2 3.4

β-HCH NA NA NA NA NA NA NA NA NA NA 409 42.5 8.00 1.4γ-HCH NA NA NA NA NA NA NA NA NA NA 6.87 1.64 3.30 0.81

Heptachlor Epoxide NA NA NA NA NA NA NA NA NA NA 99.8 18 10.8 1.3

Cis-Chlordane NA NA NA NA NA NA NA NA NA NA 37.4 5.4 46.9 2.8

Trans-Chlordane NA NA NA NA NA NA NA NA NA NA 11.5 8.2 12.9 3.5Oxychlordane NA NA NA NA NA NA NA NA NA NA 78.9 7.4 19.8 1.9

Cis-Nonachlor NA NA NA NA NA NA NA NA NA NA 98.4 17 48.7 7.6

Trans-Nonachlor NA NA NA NA NA NA NA NA NA NA 1238 150 231 11Dieldrin NA NA NA NA NA NA NA NA NA NA 209 56 37.5 3.9Mirex NA NA NA NA NA NA NA NA NA NA 43.7 7.5 18.9 2.8

Category z (2

≤ 2 

2 to 3 

≥ 3 

z (2

Lipid (mass fraction (%)) 7.67 8.90 7.44 70.4 81.2 69.2 8.0 9.81 73.6 9.03 6.4 2.08 74.3 0.45 1

*Certified values are in bold **See text for explaination

Homog. V SRM 1945 SRM 1945 Homog. V

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass)

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQA V AND SRM 1945

data are reported as if three figures are significant

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 7/1/2001 7/1/2001 7/1/2001 7/1/2001 7/1/2001 7/1/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

18 <10 <10 <10 3.50 4.10 NA <10 <10 3.80 11 7.73 1.5 4.48 0.8828 <10 <10 <10 17.6 18.9 NA <10 <10 18.3 5.0 4.26 2.3 14.10 1.431 NA NA NA NA NA NA NA NA NA NA 3.28 2.6 3.12 0.6944 30.9 40.5 52.5 15.7 16.8 NA 41.3 26 16.3 4.8 35.3 3.8 12.2 1.4 049 95.6 109 125 18.6 22.4 NA 109.9 13.4 20.5 13 86.1 11 20.8 2.8 152 160 163 187 38.8 45.4 NA 170 8.7 42.1 11 143 8.5 43.6 2.5 066/95* 199 228 302 63.3 65.7 NA 243.0 22 64.5 2.6 192 21 57.4 2.5 187 160 178 242 22.0 28.0 NA 193 22 25.0 17 157 20 16.7 1.4 099 538 502 575 85.0 93.0 NA 538 6.8 89.0 6.4 467 34 45.4 5.4 0101 (+90) 320 341 447 59.0 69.0 NA 369 18.4 64.0 11 410 53 65.2 5.6 -0105 203 249 313 39.5 43.1 NA 255 22 41.3 6.2 161 19 30.1 2.3 2118 563 547 660 72.0 83.0 NA 590 10 77.5 10 511 59 74.6 5.1 0128 160 154 181 31.0 35.0 NA 165 8.6 33.0 8.6 178 25 23.7 1.7 -0132 NA NA NA NA NA NA NA NA NA NA 160 68 29.1 6.0138 (+163+164) 1401 1537 1764 155 168 NA 1567 12 162 5.7 1104 213 131.5 7.4 1149 NA NA NA NA NA NA NA NA NA NA 320 43 107 8.4

151 121 123 134 26.0 32.0 NA 126 5.6 29.0 15 134 20 28.7 5.2 -0153 1828 1898 2298 228 247 NA 2008 13 238 5.7 1833 199 213.0 19 0156 85.0 100 119 41.0 49.0 NA 101.3 17 45.0 13 18.5 11 10.3 1.1 17170 (+190) 247 263 308 46.0 53.0 NA 273 12 49.5 10 218 27 40.6 2.6 1180 683 725 780 118 131 NA 729 6.7 125 7.4 798 87 107 5.3 -0183 176 210 235 41.0 43.0 NA 207 14 42.0 3.4 231 30 36.6 4.1 -0187 439 470 542 102 110 NA 484 11 106 5.3 436 62 105 9.1 0194 152 146 153 43.0 47.0 NA 150 2.5 45.0 6.3 165 16 39.6 2.5 -0195 77.7 75.5 90.4 19.0 27.0 NA 81.2 9.9 23.0 25 40.0 9.3 17.7 4.3 4201 54.0 58.0 61.0 23.0 27.0 NA 57.7 6.1 25.0 11 87.7 52 17.0 0.89 -1206 79.3 76.8 89.0 31.0 35.0 NA 81.7 7.9 33.0 8.6 83.1 7.8 31.1 2.7 -0

209 50.0 53.0 61.0 12.0 14.0 NA 54.7 10 13.0 10.9 69.4 7.2 10.6 1.1 -066 NA NA NA NA NA NA NA NA NA NA 50.7 4.3 23.6 1.695 NA NA NA NA NA NA NA NA NA NA 141 15 33.8 1.7

*Certified values are in bold **See text for explaination Category z (2

≤ 2 2

2 to 3 

≥ 3 

Homog. V SRM 1945 Homog. V SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass) 

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Laboratory 

SAMPLES: MMQAV AND SRM 1945

data are reported as if three figures are significant

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-score 

Date(s) of measurements 7/4-5/01 7/4-5/02 7/4-5/03 7/4-5/04 7/4-5/05 7/4-5/06 ng/g wet %RSD ng/g wet %RSD Value Value (25%) 

2,4’-DDT 102.0 87.0 97.0 56.7 58.9 57.5 95.3 8.0 57.7 1.9 83.1 22 106 14 0.6 

4,4’-DDT 223 181 210 157 170 158 205 10.5 162 4.5 184 21 245 15 0.5 

2,4’-DDE 9.20 10.8 9.20 115 90.0 106 9.73 9.5 104 12 110 28 12.3 0.87 -3.6 

4,4’-DDE 39100 35300 30100 362 379 390 34833 13 377 3.7 33401 6050 445 37 0.2 

2,4’-DDD 28.0 22.0 24.0 10.5 11.5 10.2 24.7 12 10.7 6.3 45.7 14 18.1 2.8 -1.8 

4,4’-DDD 1060 1040 1020 82.4 88.7 85.6 1040 1.9 86 3.7 1005 138 133 10 0.1 

HCB <5 <5 <5 20.0 16.7 17.0 <5 <5 17.9 10 1.89 1.4 32.9 1.7

α-HCH 7.10 6.20 6.80 16.9 15.1 14.2 6.70 6.8 15.4 8.9 6.43 1.0 16.2 3.4 0.2 

β-HCH 460 462 463 <10 <10 <10 462 0.3 <10 <10 409 42.5 8.00 1.4 0.5 

γ-HCH <5 <5 <5 <5 <5 <5 <5 <5 <5 <5 6.87 1.64 3.30 0.81

Heptachlor Epoxide 150 123 130 17.6 13.5 12.9 134 10 14.7 17 99.8 18 10.8 1.3 1.4 

Cis-Chlordane 80.0 63.0 71.0 47.4 44.1 39.7 71.3 12 43.7 8.8 37.4 5.4 46.9 2.8 3.6 

Trans-Chlordane <5 <5 <5 <5 <5 <5 <5 <5 <5 <5 11.5 8.2 12.9 3.5Oxychlordane 99.0 78.0 74.0 25.2 19.9 18.1 83.7 16.1 21.1 17.5 78.9 7.4 19.8 1.9 0.2 

Cis-Nonachlor 107 96.0 108 57.7 58.5 57.0 104 6.4 57.7 1.3 98.4 17 48.7 7.6 0.2 

Trans-Nonachlor 1660 1610 1560 164 169 155 1610 3.1 163 4.4 1238 150 231 11 1.2 

Dieldrin 65 60 56 17.5 24.1 13.4 60.3 7.5 18.3 29 209 56 37.5 3.9 -2.8 Mirex 29.0 22.0 26.0 23.1 17.6 18.6 25.7 14 19.8 15 43.7 7.5 18.9 2.8 -1.7 

Category z (25%) 

≤ 2 12 

2 to 3 1

≥ 3 2

z (25%) 

Lipid (mass fraction (%)) 8.04 7.55 7.20 74.4 76.4 72.7 7.6 5.55 74.5 2.49 6.4 2.08 74.3 0.45 0.7 

*Certified values are in bold **See text for explaination

Homog. V SRM 1945 SRM 1945 Homog. V

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass)

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Laboratory 

SAMPLES: MMQA V AND SRM 1945

data are reported as if three figures are significant

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-score 

Date(s) of measurements 5/21/2001 5/21/2001 5/21/2001 5/21/2001 5/21/2001 5/21/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25%) 

18 NA NA NA NA NA NA NA NA NA NA 7.73 1.5 4.48 0.88

28 3.31 2.98 2.87 16.8 11.7 15.7 3.05 7.5 14.7 18.2 4.26 2.3 14.10 1.4 -1.1 31 0.642 0.7 0.809 5.18 4.31 5.63 0.7 12 5.0 13 3.28 2.6 3.12 0.69 -3.1 44 NA NA NA NA NA NA NA NA NA NA 35.3 3.8 12.2 1.4

49 41.6 36.6 37.4 8.5 6.2 8.0 38.5 7.0 7.6 16.0 86.1 11 20.8 2.8 -2.2 

52 142 128 125 42.7 31.4 40.9 132 6.9 38.3 15.8 143 8 43.6 2.5 -0.3 

66/95* NA NA NA NA NA NA NA NA NA NA 192 21 57.4 2.5

87 0.118 0.166 <0.1 11.4 8.7 11.0 0.142 23.9 10.4 13.9 157 20 16.7 1.4 -4.0 

99 NA NA NA NA NA NA NA NA NA NA 467 34 45.4 5.4

101 (+90) NA NA NA NA NA NA NA NA NA NA 410 53 65.2 5.6

105 98 85 88 17.6 13.7 17.6 90 7.6 16.3 13.8 161 19 30.1 2.3 -1.8 

118 168 166 196 66.1 49.1 63.2 177 9.5 59.5 15.3 511 59 74.6 5.1 -2.6 

128 148 147 144 22.1 16.6 21.4 146 1.4 20.0 14.9 178 25 23.7 1.7 -0.7 

132 NA NA NA NA NA NA NA NA NA NA 160 68 29.1 6.0138 (+163+164) 110 136 104 78 60 77 117 14.6 72 13.8 1104 213 131.5 7.4 -3.6 

149 NA NA NA NA NA NA NA NA NA NA 320 43 107 8.4

151 NA NA NA NA NA NA NA NA NA NA 134 20 28.7 5.2

153 122 113 121 168 121 162 119 4.2 150 17.0 1833 199 213.0 19 -3.7 

156 10.2 7.8 8.1 16.6 12.0 16.2 8.7 15.0 14.9 17.1 18.5 11 10.3 1.1 -2.1 

170 (+190) 124 109 108 32.6 23.0 29.6 114 7.9 28.4 17.3 218 27 40.6 2.6 -1.9 

180 NA NA NA NA NA NA NA NA NA NA 798 87 107 5.3

183 NA NA NA NA NA NA NA NA NA NA 231 30 36.6 4.1

187 NA NA NA NA NA NA NA NA NA NA 436 62 105 9.1

194 NA NA NA NA NA NA NA NA NA NA 165 16 39.6 2.5

195 NA NA NA NA NA NA NA NA NA NA 40.0 9 17.7 4.3

201 NA NA NA NA NA NA NA NA NA NA 87.7 52 17.0 0.89

206 NA NA NA NA NA NA NA NA NA NA 83.1 7.8 31.1 2.7

209 NA NA NA NA NA NA NA NA NA NA 69.4 7.2 10.6 1.166 57.7 52.2 53.2 22.9 16.3 20.8 54.4 5.4 20.0 16.9 50.7 4 23.6 1.6 0.3 95 NA NA NA NA NA NA NA NA NA NA 141 15 33.8 1.7

*Certified values are in bold **See text for explaination Category z (25%) 

≤ 2 6 

2 to 3 3

≥ 3 4

Homog. V SRM 1945 Homog. V SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass) 

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQAV AND SRM 1945

data are reported as if three figures are significant

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 10/17/2001 10/17/2001 10/17/2001 10/17/2001 10/17/2001 10/17/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

2,4’-DDT 70.0 78.0 71.0 130.0 110.0 110.0 73.0 6.0 116.7 9.9 83.1 22 106 14 -04,4’-DDT 140 170 150 220 160 170 153 10 183 18 184 21 245 15 -0

2,4’-DDE 210 220 190 9.00 7.70 8.20 207 7.4 8.30 7.9 110 28 12.3 0.87 34,4’-DDE 5500 5500 5300 510 480 480 5433 2.1 490 3.5 33401 6050 445 37 -32,4’-DDD 39.0 48.0 41.0 20.0 18.0 17.0 42.7 11 18.3 8.3 45.7 14 18.1 2.8 -04,4’-DDD 1000 1200 1200 130 110 110 1133 10 117 9.9 1005 138 133 10 0HCB 4.50 2.40 2.40 24.0 20.0 23.0 3.10 39 22.3 9.3 1.89 1.4 32.9 1.7 2α-HCH 4.80 4.30 4.90 10.0 8.10 8.40 4.67 6.9 8.83 12 6.43 1.0 16.2 3.4 -1

β-HCH 590 700 640 4.80 4.00 3.40 643 8.6 4.07 17 409 43 8.00 1.4 2γ-HCH 3.60 3.60 4.00 2.00 2.20 2.50 3.73 6.2 2.23 11 6.87 1.64 3.30 0.81 -1

Heptachlor Epoxide 43.0 51.0 49.0 5.20 4.40 4.30 47.7 8.7 4.6 11 99.8 18 10.8 1.3 -2Cis- Chlordane 26.0 34.0 31.0 48.0 39.0 39.0 30.3 13 42.0 12 37.4 5.4 46.9 2.8 -0

Trans- Chlordane 5.70 7.20 7.20 10.0 8.50 8.80 6.70 13 9.10 8.7 11.5 8.2 12.9 3.5 -1Oxychlordane 70.0 80.0 78.0 24.0 19.0 22.0 76.0 7.0 21.7 12 78.9 7.4 19.8 1.9 -0Cis- Nonachlor 79.0 96.0 90.0 44.0 35.0 37.0 88.3 9.8 38.7 12 98.4 17 48.7 7.6 -0Trans- Nonachlor 1100 1200 1100 170 160 160 1133 5.1 163 3.5 1238 150 231 11 -0Dieldrin 180 210 200 51.0 37.0 40.0 197 7.8 42.7 17 209 56 37.5 3.9 -0Mirex 25.0 27.0 23.0 9.10 8.40 8.00 25.0 8.0 8.50 6.6 43.7 7.5 18.9 2.8 -1

Category z (2

≤ 2 1

2 to 3 

≥ 3 

z (2

Lipid (mass fraction (%)) 6.60 8.20 7.80 83.0 76.7 75.4 7.5 11.05 78.4 5.19 6.4 2.08 74.3 0.45 0

*Certified values are in bold **See text for explaination

Homog. V SRM 1945 SRM 1945 Homog. V

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass)

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQA V AND SRM 1945

data are reported as if three figures are significant

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 10/17/2001 10/17/2001 10/17/2001 10/17/2001 10/17/2001 10/17/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

18 5.60 5.70 5.30 2.70 2.60 2.60 5.53 3.8 2.63 2.2 7.73 1.5 4.48 0.88 -128 4.60 4.70 4.40 11.0 11.0 11.0 4.57 3.3 11.0 0.0 4.26 2.3 14.10 1.4 031 2.20 2.50 2.40 3.50 3.10 3.20 2.37 6.5 3.27 6.4 3.28 2.6 3.12 0.69 -144 30.0 30.0 27.0 11.0 9.9 10.0 29.0 6.0 10.3 5.9 35.3 3.8 12.2 1.4 -049 66.0 67.0 62.0 23.0 21.0 20.0 65.0 4.1 21.3 7.2 86.1 11 20.8 2.8 -152 110 120 110 37.0 36.0 36.0 113 5.1 36.3 1.6 143 8 43.6 2.5 -066/95* see below see below see below see below see below see below 192 21 57.4 2.587 120 130 120 16.0 19.0 16.0 123 4.7 17.0 10 157 20 16.7 1.4 -099 360 380 370 56.0 49.0 48.0 370 2.7 51.0 8.5 467 34 45.4 5.4 -0101 (+90) 290 300 290 65.0 62.0 62.0 293 2.0 63.0 2.7 410 53 65.2 5.6 -1105 180 180 160 51.0 48.0 43.0 173 6.7 47.3 8.5 161 19 30.1 2.3 0118 480 490 460 80.0 75.0 75.0 477 3.2 76.7 3.8 511 59 74.6 5.1 -0128 170 170 160 27.0 23.0 24.0 167 3.5 24.7 8.4 178 25 23.7 1.7 -0132 91.0 95.0 87.0 20.0 18.0 17.0 91.0 4.4 18.3 8.3 160 68 29.1 6.0 -1138 (+163+164) 860 890 840 130 120 120 863 2.9 123 4.7 1104 213 131.5 7.4 -0149 270 280 260 83.0 79.0 79.0 270 3.7 80.3 2.9 320 43 107 8.4 -0151 260 200 190 25.0 23.0 24.0 217 17 24.0 4.2 134 20 28.7 5.2 2153 1500 1500 1500 200 180 180 1500 0.0 187 6.2 1833 199 213.0 19 -0156 6.50 6.40 6.20 13.0 12.0 12.0 6.37 2.4 12.3 4.7 18.5 11 10.3 1.1 -2170 (+190) 240 240 220 43.0 41.0 40.0 233 4.9 41.3 3.7 218 27 40.6 2.6 0180 720 730 700 140 130 130 717 2.1 133 4.3 798 87 107 5.3 -0183 200 200 190 35.0 32.0 33.0 197 2.9 33.3 4.6 231 30 36.6 4.1 -0187 380 400 380 110 100 110 387 3.0 107 5.4 436 62 105 9.1 -0194 160 160 150 60.0 57.0 55.0 157 3.7 57.3 4.4 165 16 39.6 2.5 -0195 42.0 40.0 38.0 12.0 12.0 12.0 40.0 5.0 12.0 0.0 40.0 9 17.7 4.3 0201 93.0 92.0 85.0 44.0 40.0 40.0 90.0 4.8 41.3 5.6 87.7 52 17.0 0.89 0206 86.0 87.0 80.0 72.0 62.0 62.0 84.3 4.5 65.3 8.8 83.1 7.8 31.1 2.7 0

209 NA NA NA NA NA NA NA NA NA NA 69.4 7.2 10.6 1.1

66 42.0 43.0 40.0 23.0 21.0 21.0 41.7 3.7 21.7 5.3 50.7 4 23.6 1.6 -095 110 110 92 31.0 29.0 32.0 104 10 30.7 5.0 141 15 33.8 1.7 -1

*Certified values are in bold **See text for explaination Category z (2

≤ 2 2

2 to 3 

≥ 3 

Homog. V SRM 1945 Homog. V SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass) 

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQAV AND SRM 1945

data are reported as if three figures are significant

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 10/26/2001 10/26/2001 10/26/2001 10/26/2001 10/26/2001 10/26/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

2,4’-DDT 65.8 63.3 64.1 99.4 98.6 90.5 64.4 2.0 96.2 5.1 83.1 22 106 14 -04,4’-DDT 195 199 187 231 228 237 194 3.2 232 2.0 184 21 245 15 0

2,4’-DDE 124 120 121 12.6 12.9 12.8 122 1.7 12.8 1.2 110 28 12.3 0.87 04,4’-DDE 40888 39677 41129 466 469 460 40565 1.9 465 1.0 33401 6050 445 37 02,4’-DDD 33.7 36.0 33.0 17.3 17.5 17.1 34.2 4.6 17.3 1.2 45.7 14 18.1 2.8 -14,4’-DDD 1191 1228 1180 123 126 129 1200 2.1 126 2.4 1005 138 133 10 0HCB <5 <5 <5 30.3 31.1 30.5 <5 <5 30.6 1.4 1.89 1.4 32.9 1.7α-HCH <5 <5 <5 15.6 16.2 16.1 <5 < 16.0 2.0 6.43 1.0 16.2 3.4

β-HCH interferenceinterferenceinterferenceinterferenceinterferenceinterference 409 42.5 8.00 1.4γ-HCH 12.50 13.20 13.90 3.17 3.17 3.58 13.2 5.3 3.31 7.2 6.87 1.64 3.30 0.81 3

Heptachlor Epoxide 134 128 127 10.7 10.3 10.6 130 2.9 10.5 2.0 99.8 18 10.8 1.3 1Cis-Chlordane 61.3 58.6 63.2 49.4 48.3 47.6 61.0 3.8 48.4 1.9 37.4 5.4 46.9 2.8 2Trans-Chlordane 39.5 41.9 43.2 10.7 11.8 11.3 41.5 4.5 11.3 4.9 11.5 8.2 12.9 3.5 10Oxychlordane 66.1 66.1 64.7 19.6 18.1 19.7 65.6 1.2 19.1 4.7 78.9 7.4 19.8 1.9 -0Cis-Nonachlor 118 120 116 48.9 44.7 50.5 118 1.7 48.0 6.2 98.4 17 48.7 7.6 0Trans-Nonachlor 1436 1430 1382 214 211 200 1416 2.1 208 3.5 1238 150 231 11 0Dieldrin 252 245 249 38.1 38.9 40.1 249 1.4 39.0 2.6 209 56 37.5 3.9 0Mirex 42.0 39.6 40.4 29.5 28.6 28.7 40.7 3.0 28.9 1.7 43.7 7.5 18.9 2.8 -0

Category z (2

≤ 2 1

2 to 3 

≥ 3 

z (2

Lipid (mass fraction (%)) 6.54 6.82 6.52 74.3 74.4 74.4 6.6 2.53 74.4 0.09 6.4 2.08 74.3 0.45 0

*Certified values are in bold **See text for explaination

Homog. V SRM 1945 SRM 1945 Homog. V

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass)

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Labor

SAMPLES: MMQA V AND SRM 1945

data are reported as if three figures are significant

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-s

Date(s) of measurements 10/26/2001 10/26/2001 10/26/2001 10/26/2001 10/26/2001 10/26/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25

18 6.04 6.19 6.14 5.04 5.11 4.93 6.12 1.2 5.03 1.8 7.73 1.5 4.48 0.88 -028 3.01 3.11 2.44 12.5 12.9 12.6 2.85 12.7 12.7 1.6 4.26 2.3 14.10 1.4 -131 1.77 1.72 1.69 3.28 3.22 3.26 1.7 2.3 3.3 1 3.28 2.6 3.12 0.69 -144 36.5 37.5 34.0 11.7 11.9 11.4 36.0 5.0 11.7 2.2 35.3 3.8 12.2 1.4 049 coelution coelution coelution coelution coelution coelution 86.1 11 20.8 2.8

52 140 144 135 41.7 43.7 43.2 140 3.2 42.9 2.4 143 8 43.6 2.5 -066/95* see below see below see below see below see below see below 192 21 57.4 2.587 124 128 127 16.0 16.1 15.9 126 1.6 16.0 0.6 157 20 16.7 1.4 -099 425 443 422 49.7 50.9 48.7 430 2.6 49.8 2.2 467 34 45.4 5.4 -0101 (+90) 405 429 415 70.6 70.5 68.3 416 2.9 69.8 1.9 410 53 65.2 5.6 0105 138 137 136 28.8 29.5 29.4 137 0.7 29.2 1.3 161 19 30.1 2.3 -0118 429 427 409 74.6 71.5 75.2 422 2.6 73.8 2.7 511 59 74.6 5.1 -0128 142 148 145 19.1 20.3 20.1 145 2.1 19.8 3.2 178 25 23.7 1.7 -0132 118 114 110 17.4 17.7 17.2 114 3.5 17.4 1 160 68 29.1 6.0 -1138 (+163+164) coelution coelution coelution coelution coelution coelution 1104 213 131.5 7.4149 276 271 261 94.2 94.6 96.3 269 2.8 95.0 1.2 320 43 107 8.4 -0151 115 119 109 27.2 27.4 28.0 114 4.4 27.5 1.5 134 20 28.7 5.2 -0153 1540 1509 1469 216 213 217 1506 2.4 215 1.0 1833 199 213.0 19 -0156 7.91 7.41 7.59 9.64 9.43 9.71 7.64 3.3 9.6 1.5 18.5 11 10.3 1.1 -2170 (+190) 183 187 185 39.6 41.7 39.4 185 1.1 40.2 3.2 218 27 40.6 2.6 -0180 coelution coelution coelution coelution coelution coelution 798 87 107 5.3183 165 161 161 35.9 34.7 34.0 162 1.4 34.9 2.8 231 30 36.6 4.1 -1187 334 335 326 100 94 94 332 1.5 96 3.3 436 62 105 9.1 -1194 124 125 124 38.3 38.2 39.5 124 0.5 38.7 1.9 165 16 39.6 2.5 -1195 31.2 31.8 28.2 16.2 17.0 16.8 30.4 6.3 16.7 2.5 40.0 9 17.7 4.3 -1201 29.4 32.8 29.2 17.9 16.5 16.5 30.5 6.6 17.0 4.8 87.7 52 17.0 0.89 -2206 66.5 63.6 64.0 35.9 35.6 34.3 64.7 2.4 35.3 2.4 83.1 7.8 31.1 2.7 -0

209 57.6 52.7 55.4 10.0 10.4 10.6 55.2 4.4 10.3 3.0 69.4 7.2 10.6 1.1 -066 54.2 55.2 51.0 23.1 22.1 22.9 53.5 4.1 22.7 2.3 50.7 4 23.6 1.6 095 147 155 148 34.2 33.8 34.9 150 2.9 34.3 1.6 141 15 33.8 1.7 0

*Certified values are in bold **See text for explaination Category z (2

≤ 2 2

2 to 3 

≥ 3 

Homog. V SRM 1945 Homog. V SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass) 

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Laboratory 

SAMPLES: MMQAV AND SRM 1945

data are reported as if three figures are significant

PESTICIDE AND 

LIPID ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-score 

Date(s) of measurements 7/19/2001 7/19/2001 7/20/2001 7/19/2001 7/19/2001 7/19/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25%) 

2,4’-DDT NA NA NA NA NA NA NA NA NA NA 83.1 22 106 14

4,4’-DDT 36 40 29 44 49 47 35 15.9 47 5.4 184 21 245 15 -3.2 

2,4’-DDE NA NA NA NA NA NA NA NA NA NA 110 28 12.3 0.87

4,4’-DDE 22000 23000 23000 430 350 290 22667 2.5 357 20 33401 6050 445 37 -1.3 

2,4’-DDD NA NA NA NA NA NA NA NA NA NA 45.7 14 18.1 2.8

4,4’-DDD NA NA NA NA NA NA NA NA NA NA 1005 138 133 10

HCB NA NA NA NA NA NA NA NA NA NA 1.89 1.4 32.9 1.7

α-HCH NA NA NA NA NA NA NA NA NA NA 6.43 1.0 16.2 3.4β-HCH NA NA NA NA NA NA NA NA NA NA 409 42.5 8.00 1.4

γ-HCH NA NA NA NA NA NA NA NA NA NA 6.87 1.64 3.30 0.81

Heptachlor Epoxide NA NA NA NA NA NA NA NA NA NA 99.8 18 10.8 1.3

Cis- Chlordane NA NA NA NA NA NA NA NA NA NA 37.4 5.4 46.9 2.8

Trans- Chlordane NA NA NA NA NA NA NA NA NA NA 11.5 8.2 12.9 3.5Oxychlordane NA NA NA NA NA NA NA NA NA NA 78.9 7.4 19.8 1.9

Cis- Nonachlor NA NA NA NA NA NA NA NA NA NA 98.4 17 48.7 7.6

Trans- Nonachlor NA NA NA NA NA NA NA NA NA NA 1238 150 231 11

Dieldrin NA NA NA NA NA NA NA NA NA NA 209 56 37.5 3.9Mirex NA NA NA NA NA NA NA NA NA NA 43.7 7.5 18.9 2.8

Category z (25%) 

≤ 2 1

2 to 3 0

≥ 3 1

z (25%) 

Lipid (mass fraction (%)) 10.00 10.00 10.00 74.4 74.6 74.6 10.0 0.00 74.5 0.15 6.4 2.08 74.3 0.45 2.2 

*Certified values are in bold **See text for explaination

Homog. V SRM 1945 SRM 1945 Homog. V

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass)

Homog V SRM 1945
2001 EXERCISE FOR ORGANOCHLORINES IN MARINE MAMMAL TISSUES Laboratory 

SAMPLES: MMQA V AND SRM 1945

data are reported as if three figures are significant

PCB CONGENER 

ANALYSES Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3 lab mean lab lab mean lab Assigned 95% CL Target* 95% CL z-score 

Date(s) of measurements 7/19/2001 7/19/2001 7/20/2001 7/19/2001 7/19/2001 7/19/2001 ng/g wet %RSD ng/g wet %RSD Value Value (25%) 

18 NA NA NA NA NA NA NA NA NA NA 7.73 1.5 4.48 0.88

28 NA NA NA NA NA NA NA NA NA NA 4.26 2.3 14.10 1.431 NA NA NA NA NA NA NA NA NA NA 3.28 2.6 3.12 0.6944 NA NA NA NA NA NA NA NA NA NA 35.3 3.8 12.2 1.4

49 NA NA NA NA NA NA NA NA NA NA 86.1 11 20.8 2.8

52 180 160 150 33.0 47.0 38.0 163 9.4 39.3 18 143 8 43.6 2.5 0.6 

66/95* see below see below see below see below see below see below 192 21 57.4 2.5 -4.0 

87 NA NA NA NA NA NA NA NA NA NA 157 20 16.7 1.4

99 460 410 420 54.0 51.0 61.0 430 6.2 55.3 9.3 467 34 45.4 5.4 -0.3 

101 (+90) 440 470 520 66.0 81.0 81.0 477 8.5 76.0 11 410 53 65.2 5.6 0.7 

105 170 160 180 17.0 25.0 21.0 170 5.9 21.0 19 161 19 30.1 2.3 0.2 

118 520 520 580 66.0 71.0 74.0 540 6.4 70.3 5.7 511 59 74.6 5.1 0.2 

128 NA NA NA NA NA NA NA NA NA NA 178 25 23.7 1.7

132 NA NA NA NA NA NA NA NA NA NA 160 68 29.1 6.0138 (+163+164) 1800 1400 1500 210 210 230 1567 13 217 5.3 1104 213 131.5 7.4 1.7 

149 350 280 310 71.0 62.0 62.0 313 11 65.0 8.0 320 43 107 8.4 -0.1 151 100 120 140 17.0 19.0 23.0 120 17 19.7 16 134 20 28.7 5.2 -0.4 

153 2000 1600 2000 230 190 220 1867 12 213 9.8 1833 199 213.0 19 0.1 

156 NA NA NA NA NA NA NA NA NA NA 18.5 11 10.3 1.1

170 (+190) 190 260 250 51.0 40.0 30.0 233 16 40.3 26 218 27 40.6 2.6 0.3 

180 720 820 820 220 140 140 787 7.3 167 28 798 87 107 5.3 -0.1 183 270 220 240 62.0 45.0 34.0 243 10 47.0 30 231 30 36.6 4.1 0.2 

187 570 550 530 230 150 140 550 3.6 173 28 436 62 105 9.1 1.0 194 NA NA NA NA NA NA NA NA NA NA 165 16 39.6 2.5

195 NA NA NA NA NA NA NA NA NA NA 40.0 9 17.7 4.3

201 NA NA NA NA NA NA NA NA NA NA 87.7 52 17.0 0.89

206 NA NA NA NA NA NA NA NA NA NA 83.1 7.8 31.1 2.7

209 NA NA NA NA NA NA NA NA NA NA 69.4 7.2 10.6 1.166 NA NA NA NA NA NA NA NA NA NA 50.7 4 23.6 1.695 110 110 120 26.0 27.0 43.0 113 5.1 32.0 30 141 15 33.8 1.7 -0.8 

*Certified values are in bold **See text for explaination Category z (25%) 

≤ 2 14 

2 to 3 0

≥ 3 1

Homog. V SRM 1945 Homog. V SRM 1945

Data as Submitted by Laboratory (ng/g wet mass) Values for Materials (ng/g wet mass) 

Homog V SRM 1945
APPENDIX B 

Graphical results of PCB congener and lipid data reported by all laboratories. The Zscores for Control Material V represent 25% of the assigned value so that Z = +1 is the 

assigned value plus 25 %, Z = -1 is the assigned value minus 25 % and so forth. Error bars 

are ± 1 standard deviation. 
Control Material V (MMQAV)

PCB 18

Assigned value = 7.73 ng/g s = 2.2 ng/g 95% CI = ± 1.5 ng/g (wet basis)

Reported Results: 10 Quantitative Results: 9

0

2

4

6

8

10

12

14

16

18

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 18

Value = 4.48 ± 0.88 ng/g (wet basis)

Reported Results: 11 

0

1

2

3

4

5

6

7

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 28

Assigned value = 4.26 ng/g s = 3.9 ng/g 95% CI = ±2.3 ng/g (wet basis)

Reported Results: 12 Quantitative Results: 11

0

2

4

6

8

10

12

14

16

18

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 28

Value = 14.1 ± 1.4 ng/g (wet basis)

Reported Results: 15 

0

5

10

15

20

25

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 31

Assigned value = 3.28 ng/g s = 3 .3 ng/g 95% CI = ±2.64 ng/g (wet basis)

Reported Results: 6 Quantitative Results: 6

0

2

4

6

8

10

12

14

16

18

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 31

Value = 3.12 ± 0.69 ng/g (wet basis)

Reported Results: 10 

0

1

2

3

4

5

6

7

8

9

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 44

Assigned value = 35.3 ng/g s = 7.8 ng/g 95% CI = ±3.9 ng/g (wet basis)

Reported Results: 16 Quantitative Results: 16

0

10

20

30

40

50

60

70

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 44

Value = 12.2 ± 1.4 ng/g (wet basis)

Reported Results: 15 

0

5

10

15

20

25

30

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 49

Assigned value = 86.1 ng/g s = 22 ng/g 95% CI = ± 11 ng/g (wet basis)

Reported Results: 15 Quantitative Results: 15

0

20

40

60

80

100

120

140

160

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 49

Value = 20.8 ± 2.8 ng/g (wet basis)

Reported Results: 15 

0

5

10

15

20

25

30

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 52

Assigned value = 143 ng/g s = 17 ng/g 95% C = ± 8.5 ng/g (wet basis)

Reported Results: 17 Quantitative Results: 16

0

50

100

150

200

250

300

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 52

Value = 43.6 ± 2.5 ng/g (wet basis)

Reported Results: 17 

0

10

20

30

40

50

60

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 66

Assigned value = 50.7 ng/g s = 7.2 ng/g 95% C = ± 4.3 ng/g (wet basis)

Reported Results: 12 Quantitative Results: 11

0

10

20

30

40

50

60

70

80

90

100

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 66

Value = 23.6 ± 1.6 ng/g (wet basis)

Reported Results: 12 

0

5

10

15

20

25

30

35

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 95

Assigned value = 141 ng/g s = 27 ng/g 95% CI = ± 15 ng/g (wet basis)

Reported Results: 13 Quantitative Results: 13

0

50

100

150

200

250

300

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 95

Value = 33.8 ± 1.7 ng/g (wet basis)

Reported Results: 13 

0

10

20

30

40

50

60

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 66/95

Assigned value = 192 ng/g s = 39 ng/g 95% CI = ± 21 ng/g (wet basis)

Reported Results: 13 Quantitative Results: 13

0

50

100

150

200

250

300

350

400

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 66/95

Value = 57.4 ± ng/g (wet basis)

Reported Results: 13 

0

10

20

30

40

50

60

70

80

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 87

Assigned value = 157 ng/g s = 31 ng/g 95% CI = ± 20 ng/g (wet basis)

Reported Results: 11 Quantitative Results: 10

0

50

100

150

200

250

300

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 87

Value = 16.7 ± 1.4 ng/g (wet basis)

Reported Results: 11 

0

5

10

15

20

25

30

35

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 99

Assigned value = 467 ng/g s = 65 ng/g 95% CI = ± 34 ng/g (wet basis)

Reported Results: 14 Quantitative Results: 14

0

100

200

300

400

500

600

700

800

900

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 99

Value = 45.4 ± 5.4 ng/g (wet basis)

Reported Results: 14 

0

10

20

30

40

50

60

70

80

90

100

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 101/90

Assigned value = 410 ng/g s = 108 ng/g 95% CI = ± 53 ng/g (wet basis)

Reported Results: 16 Quantitative Results: 16

0

100

200

300

400

500

600

700

800

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 101/90

Value = 65.2 ± 5.6 ng/g (wet basis)

Reported Results: 16 

0

20

40

60

80

100

120

140

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 105

Assigned value = 161 ng/g s = 42 ng/g 95% CI = ± 19 ng/g (wet basis)

Reported Results: 18 Quantitative Results: 18

0

50

100

150

200

250

300

350

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 105

Value = 30.1 ± 2.3 ng/g (wet basis)

Reported Results: 18 

0

10

20

30

40

50

60

70

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 118

Assigned value = 511 ng/g s = 128 ng/g 95% CI = ± 59 ng/g (wet basis)

Reported Results: 18 Quantitative Results: 18

0

100

200

300

400

500

600

700

800

900

1000

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 118

Value = 74.6 ± 5.1 ng/g (wet basis)

Reported Results: 18 

0

20

40

60

80

100

120

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 128

Assigned value = 178 ng/g s = 50 ng/g 95% CI = ± 25 ng/g (wet basis)

Reported Results: 16 Quantitative Results: 16

0

50

100

150

200

250

300

350

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 128

Value = 23.7 ± 1.7 ng/g (wet basis)

Reported Results: 15 

0

5

10

15

20

25

30

35

40

45

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 132

Assigned value = 160 ng/g s = 78 ng/g 95% CI = ± 68 ng/g (wet basis)

Reported Results: 5 Quantitative Results: 5

0

50

100

150

200

250

300

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 132

Value = 39.1 ± 16 ng/g (wet basis)

Reported Results: 5 

0

10

20

30

40

50

60

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 138/163/164

Assigned value = 1104 ng/g s = 435 ng/g 95% CI = ± 213 ng/g (wet basis)

Reported Results: 16 Quantitative Results: 16

0

500

1000

1500

2000

2500

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 138/163/164

Value = 132 ± 7.4 ng/g (wet basis)

Reported Results: 16 

0

50

100

150

200

250

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 149

Assigned value = 320 ng/g s = 85 ng/g 95% CI = ± 43 ng/g (wet basis)

Reported Results: 15 Quantitative Results: 15

0

100

200

300

400

500

600

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 149

Value = 107 ± 8.4 ng/g (wet basis)

Reported Results: 15 

0

20

40

60

80

100

120

140

160

180

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 151

Assigned value = 134 ng/g s = 39 ng/g 95% CI = ± 20 ng/g (wet basis)

Reported Results: 16 Quantitative Results: 15

0

50

100

150

200

250

300

350

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 151

Value = 28.7 ± 5.2 ng/g (wet basis)

Reported Results: 16 

0

10

20

30

40

50

60

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 153

Assigned value = 1833 ng/g s = 406 ng/g 95% CI = ± 199 ng/g (wet basis)

Reported Results: 17 Quantitative Results: 16

0

500

1000

1500

2000

2500

3000

3500

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 153

Value = 213 ± 19 ng/g (wet basis)

Reported Results: 17 

0

50

100

150

200

250

300

350

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 156

Assigned value = 18.5 ng/g s = 19 ng/g 95% CI = ± 11 ng/g (wet basis)

Reported Results: 12 Quantitative Results: 11

0

10

20

30

40

50

60

70

80

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 156

Value = 10.3 ± 1.1 ng/g (wet basis)

Reported Results: 15 

0

5

10

15

20

25

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 170/190

Assigned value = 218 ng/g s = 58 ng/g 95% CL = 27 ng/g (wet basis)

Reported Results: 18 Quantitative Results: 18

0

50

100

150

200

250

300

350

400

450

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 170/190

Value = 40.6 ± 2.6 ng/g (wet basis)

Reported Results: 18 

0

10

20

30

40

50

60

70

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 180

Assigned value = 798 ng/g s = 171 ng/g 95% CI = ± 87 ng/g (wet basis)

Reported Results: 16 Quantitative Results: 15

0

500

1000

1500

2000

2500

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 180

Value = 107 ± 5.3 ng/g (wet basis)

Reported Results: 16 

0

50

100

150

200

250

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 183

Assigned value = 231 ng/g s = 57 ng/g 95% CI = ± 30 ng/g (wet basis)

Reported Results: 15 Quantitative Results: 14

0

50

100

150

200

250

300

350

400

450

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 183

Value = 36.6 ± 4.1 ng/g (wet basis)

Reported Results: 14 

0

20

40

60

80

100

120

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 187

Assigned value = 436 ng/g s = 122 ng/g 95% CI = ± 62 ng/g (wet basis)

Reported Results: 15 Quantitative Results: 15

0

100

200

300

400

500

600

700

800

900

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 187

Value = 105 ± 9.1 ng/g (wet basis)

Reported Results: 15 

0

50

100

150

200

250

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 194

Assigned value = 165 ng/g s = 31 ng/g 95% CI = ± 16 ng/g (wet basis)

Reported Results: 15 Quantitative Results: 14

0

50

100

150

200

250

300

350

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 194

Value = 39.6 ± 2.5 ng/g (wet basis)

Reported Results: 15 

0

10

20

30

40

50

60

70

80

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 195

Assigned value = 40 ng/g s = 16 ng/g 95% CI = ± 9.3 ng/g (wet basis)

Reported Results: 12 Quantitative Results: 11

0

20

40

60

80

100

120

140

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 195

Value = 17.7 ± 4.3 ng/g (wet basis)

Reported Results: 12 

0

5

10

15

20

25

30

35

40

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 201

Assigned value = 88 ng/g s = 79 ng/g 95% CI = ± 52 ng/g (wet basis)

Reported Results: 9 Quantitative Results: 9

0

50

100

150

200

250

300

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 201

Value = 17.0 ± 0.89 ng/g (wet basis)

Reported Results: 10 

0

10

20

30

40

50

60

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 206

Assigned value = 83 ng/g s = 14 ng/g 95% CI = ± 7.8 ng/g (wet basis)

Reported Results: 12 Quantitative Results: 12

0

20

40

60

80

100

120

140

160

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 206

Value = 31.1 ± 2.7 ng/g (wet basis)

Reported Results: 12 

0

10

20

30

40

50

60

70

80

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

PCB 209

Assigned value = 69 ng/g s = 13 ng/g 95% CI = ± 7.2 ng/g (wet basis)

Reported Results: 12 Quantitative Results: 12

0

20

40

60

80

100

120

140

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

PCB 209

Value = 10.6 ± 1.1 ng/g (wet basis)

Reported Results: 12 

0

5

10

15

20

25

30

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
APPENDIX C 

Graphical results of pesticide and lipid data reported by all laboratories. The Z-scores for 

Control Material V represent 25% of the assigned value so that Z = +1 is the assigned value 

plus 25 %, Z = -1 is the assigned value minus 25 % and so forth. Error bars are ± 1
Control Material V (MMQAV)

2,4’-DDT

Assigned value = 83.1 ng/g s = 40 ng/g 95% CI = ± 22 ng/g (wet basis)

Reported Results: 13 Quantitative Results: 13

0

20

40

60

80

100

120

140

160

180

200

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

2,4’-DDT

Value = 106 ± 14 ng/g (wet basis)

Reported Results: 13 

0

20

40

60

80

100

120

140

160

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

4,4’-DDT

Assigned value = 184 ng/g s = 41 ng/g 95% CI = ± 21 ng/g (wet basis)

Reported Results: 16 Quantitative Results: 15

0

50

100

150

200

250

300

350

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

4,4’-DDT

Value = 245 ± 15 ng/g (wet basis)

Reported Results: 15 

0

50

100

150

200

250

300

350

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

2,4’-DDE

Assigned value = 110 ng/g s = 52 ng/g 95% CI = ± 28 ng/g (wet basis)

Reported Results: 13 Quantitative Results: 13

0

50

100

150

200

250

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

2,4’-DDE

Value = 12.3 ± 0.87 ng/g (wet basis)

Reported Results: 13 

0

5

10

15

20

25

30

35

40

45

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

4,4’-DDE

Assigned value = 33401 ng/g s = 12347 ng/g 95% CI = ± 6050 ng/g (wet basis)

Reported Results: 16 Quantitative Results: 16

0

10000

20000

30000

40000

50000

60000

70000

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

4,4’-DDE

Value = 445 ± 37 ng/g (wet basis)

Reported Results: 15 

0

100

200

300

400

500

600

700

800

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

2,4’-DDD

Assigned value = 46 ng/g s = 24 ng/g 95% CI = ± 14 ng/g (wet basis)

Reported Results: 12 Quantitative Results: 11

0

20

40

60

80

100

120

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

2,4’-DDD

Value = 18.1 ± 2.8 ng/g (wet basis)

Reported Results: 11 

0

5

10

15

20

25

30

35

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

4,4’-DDD

Assigned value = 1005 ng/g s = 263 ng/g 95% CI = ± 138 ng/g (wet basis)

Reported Results: 14 Quantitative Results: 14

0

200

400

600

800

1000

1200

1400

1600

1800

2000

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

4,4’-DDD

Value = 133 ± 10 ng/g (wet basis)

Reported Results: 14 

0

20

40

60

80

100

120

140

160

180

200

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

HCB

Assigned value = 1.89 ng/g s = 1.7 ng/g 95% CI = ± 1.4 ng/g (wet basis)

Reported Results: 6 Quantitative Results: 6

0

1

2

3

4

5

6

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

HCB

 Value = 33 ± 2 ng/g (wet basis)

Reported Results: 14 

0

5

10

15

20

25

30

35

40

45

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

alpha-HCH

Assigned value = 6.43 ng/g s = 1.7 ng/g 95% CI = ± 1.0 ng/g (wet basis)

Reported Results: 12 Quantitative Results: 12

0

2

4

6

8

10

12

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

alpha-HCH

Value = 16.2 ± 3.4 ng/g (wet basis)

Reported Results: 14 

0

5

10

15

20

25

30

35

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

beta-HCH

Assigned value = 409 ng/g s = 75 ng/g 95% CI = ± 43 ng/g (wet basis)

Reported Results: 12 Quantitative Results: 12

0

100

200

300

400

500

600

700

800

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

beta-HCH

Value = 8.0 ± 1.4 ng/g (wet basis)

Reported Results: 8 

0

2

4

6

8

10

12

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

Lipid

Assigned value = 6.42 % s = 2.1 % 95% CI = ± 0.99 %

Reported Results: 18 Quantitative Results: 17

0

5

10

15

20

25

30

35

40

45

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

Lipid

Value = 74.3 % ± 0.45 % 

Reported Results: 17 

 

0

5

10

15

20

25

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

Heptachlor Epoxide

Assigned value = 100 ng/g s = 31 ng/g 95% CI = ± 18 ng/g (wet basis)

Reported Results: 11 Quantitative Results: 11

0

20

40

60

80

100

120

140

160

180

200

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

Heptachlor Epoxide

Value = 10.8 ± 1.3 ng/g (wet basis)

Reported Results: 10 

 

0

2

4

6

8

10

12

14

16

18

20

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

Cis-Chlordane

Assigned value = 37.4 ng/g s = 10 ng/g 95% CI = ± 5.4 ng/g (wet basis)

Reported Results: 14 Quantitative Results: 13

0

10

20

30

40

50

60

70

80

90

100

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

Cis-Chlordane

Value = 46.8 ± 2.8 ng/g (wet basis)

Reported Results: 14 

 

0

10

20

30

40

50

60

70

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

Trans-Chlordane

Assigned value = 11.5 ng/g s = 13 ng/g 95% CI = ± 8.2 ng/g (wet basis)

Reported Results: 10 Quantitative Results: 10

0

5

10

15

20

25

30

35

40

45

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

Trans-Chlordane

Value = 12.1 ± 1.2 ng/g (wet basis)

Reported Results: 14 

 

0

5

10

15

20

25

30

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

Oxychlordane

Assigned value = 78.9 ng/g s = 13 ng/g 95% CI = ± 7.4 ng/g (wet basis)

Reported Results: 12 Quantitative Results: 12

0

20

40

60

80

100

120

140

160

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

Oxychlordane

Value = 19.8 ± 1.9 ng/g (wet basis)

Reported Results: 12 

 

0

5

10

15

20

25

30

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

Cis-Nonachlor

Assigned value = 98.4 ng/g s = 28 ng/g 95% CI = ± 17 ng/g (wet basis)

Reported Results: 12 Quantitative Results: 11

0

50

100

150

200

250

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

Cis-Nonachlor

Value = 48.7 ± 7.6 ng/g (wet basis)

Reported Results: 12 

 

0.0

20.0

40.0

60.0

80.0

100.0

120.0

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

Trans-Nonachlor

Assigned value = 1238 ng/g s = 285 ng/g 95% CI = ± 150 ng/g (wet basis)

Reported Results: 14 Quantitative Results: 14

0

500

1000

1500

2000

2500

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

Trans-Nonachlor

Value = 231 ± 11 ng/g (wet basis)

Reported Results: 14 

 

0

50

100

150

200

250

300

350

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

Dieldrin

Assigned value = 209 ng/g s = 95 ng/g 95% CI = ± 56 ng/g (wet basis)

Reported Results: 11 Quantitative Results: 11

0

50

100

150

200

250

300

350

400

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

Dieldrin

Value = 37.5 ± 3.9 ng/g (wet basis)

Reported Results: 11 

 

0

10

20

30

40

50

60

70

80

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

Mirex

Assigned value = 44 ng/g s = 12 ng/g 95% CI = ± 7.5 ng/g (wet basis)

Reported Results: 10 Quantitative Results: 10

0

10

20

30

40

50

60

70

80

90

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

Mirex

Value = 28.9 ± 2.8 ng/g (wet basis)

Reported Results: 10 

 

0

5

10

15

20

25

30

35

40

45

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

ng/g wet basis

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
Control Material V (MMQAV)

Lipid

Assigned value = 6.41 % s = 2.41 % 95% CI = ± 1.0 % (wet basis)

Reported Results: 18 Quantitative Results: 17

0

5

10

15

20

25

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

%

Assigned Value

± 1 Z

± 2 Z

± 3 Z

SRM 1945

Lipid

Value = 74.3 ± 0.45 % (wet basis)

Reported Results: 17 

 

0

20

40

60

80

100

120

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Laboratory

%

Certified or Reference Value

± Uncertainty

± 30 % of Ceritfied or Reference Value
APPENDIX D 

Tabular summary of methods used for analysis by each laboratory.
Summary of Methods Used

Lab No. Procedure used to measure Lipid

SRM 1945 Homog. V SRM 1945 Homog. V

1 1 1

Subsampled the dichloromethane extract,

evaporated the solvent and weighed the remaining

residue. wet wet

2 1.3 2.1 gravimetric; 1/10 of sample dried at 104 o

C wet wet

3 1 1

Methylene chloride extraction modified Dye and

Bligher. wet wet

4 1.7 4.9

After extraction with dichloromethane, each extract

was concentrated to 10ml. 2ml of this extract was

added to a previously weighed aluminum

weighling pan. The contents were allowed to

evaporate, and the pan was reweighed. wet wet

5 1 1

Gravimetric determination of non-volatile

extractable weight. wet wet

6 0.4 0.8

A weighed portion of the sample ground with

anhydrous Na2SO4, eluted with 1:1 DCM:hexane;

solvent removed under nitrogen. Concentrated

sample is baked at 35 C for 48 hours. The dried

sample is placed in a desiccator over sodium

sulfate to cool and then weighed. wet wet

7 5.02 4.65

Following hexane extraction, 50 mL of extract

evaported to dryness. wet wet

8 0.5 0.5 Extactable lipid. dry dry

9 1.3 4.8 Gravimetric wet wet

10 0.55 1.3

Gravimetric determination from portion of the

Soxhlet extraction. wet wet

11 0.6 0.6 Gravimetric weight of extract. wet wet

12 2.3 2.4

An aliquot of the Soxhlet extract was dried and

weighed. wet wet

13 0.25 0.5

Samples were evaporated just to dryness after

exraction, flask weighed. wet wet

14 1 1

Gravimeteric; extracts from Soxhlet extract dried

to constant weight. wet wet

15 0.2-0.8 0.2-0.8 Gravimetric of whole extract. wet wet

16 0.24 0.24 wet wet

17 0.6 1

From extract, remove 90 uL and allow to dry.

Percent lipid based on gravimetric ratios. wet wet

18 1.5 5.1 Gravimetric. wet wet

Approximate mass of 

sample extracted (g):

Were "wet" or "dry" 

samples extracted?
Summary of Methods Used

Lab No. Extraction Method Extraction Solvent

Extraction 

Time Extraction Other

1

Pressurized Fluid Extaction 

(Dionex, ASE) Dichloromethane 15 min

Sample dried with 45 g sodium sulfate, then packed

into a 33 mL PFE cell. PFE Conditions: cell temp

100 deg. C. equilibration 5 min, static time 5 min,

cell pressure 2000 psi and there were three cylces.

Samples were first run through a Gel Permeation

chromatogramphy column (PL Gel 600 mm) using

DCM.

2

Accelerated Solvent 

Extraction Dichloromethane 20 min

3

NOAA Technical 

Memorandum NOS ORCA 

71 Volume IV, Chapter 2 Dichloromethane

Sodium sulfate added to samples prior to

extraction; Silica/Alumina and HPLC-SEC

chromatography; see reference above.

4 Soxhlet extraction Dichloromethane 18 h

Each extract was eluted through a glass

chromatography column packed with florisil,

silica,and alumina and was concentrated to 10 mL.

2 mL of each extract was taken for the lipid

determination. The volume of the remaining eluate

of Homogenate V was adjusted to 10 mL and split

into two 5mL portions. Final sample cleanup was

done using 2 Phenogel size exclusion HPLC

columns in series. The two resulting extracts 5 mL

sample was injected into the HPLC columns by

manually over-filling a 5 mL loop with 6 mL of

sample. The extracts were combined and

concentrated to 1 mL for initial GC screening, then

diluted to 10 mL for final analysis.

5 Soxhlet Dichloromethane 12 h

6

Weighed sample is spiked

with I.S., ground with 

anhydrous Na2SO4, 

transferred to a column and 

eluted with solvent.

1:1 

dichloromethane:hexane

approximately 1 

h

7 Soxhlet n Hexane 4 h

8

Soxhlet extraction with 

mixed solvent system 

(hexane:acetone) Hexane:acetone (4:1) 6 h

9 ASE

Dichloromethane + 

acetone (1:1) 5 min x 3

10 Soxhlet

Mixture of hexane: 

diethylether (1:3) 8 h Sample was ground with anhydrous sodium sulfate. 

11 Tissumizer Dichloromethane 3 min x 3 NaSO4

12 Soxhlet

Dichloromethane:hexane 

(4:1) 7 h

13

Soxhlet, with sodium 

sulphate added to sample Dichloromethane 18 h

14 Soxhlet

Dichloromethane and 

hexane (3:1) 16 h

15

Soxhlet; PCB's, Pesticides 

ASE

PCBs, Hexane: 

Pesticides, 1:1 Hexane : 

Acetone 7 h

16 Blubber extraction Dichloromethane:hexane

17 PFE Dichloromethane approx. 15 min 100 o

C, 2000 psi, 3 cycles

18 Modified soxhlet extractor Dichloromethane/hexane 6 h
Summary of Methods Used

Lab No. Instrument Column Phase Length (m) Column i.d. (mm) Film Thickness (um) Points Co

1 Pest. GC-MS DB-5ms 60 0.25 0.25 5 1-1

PCB GC-MS DB-5ms 60 0.25 0.25 5 1-1

2 Pest. GC/MS EI DB-5 30 0.25 0.25 5 0

PCB 5 0

3 Pest. GC-MS (SIM) DB-5 60 0.25 0.25 10

0

PCB GC-MS (SIM) DB-5 60 0.25 0.25 8 0.00

4 Pest. GC-ECD DB-5 60 0.25 0.25 1 10

PCB GC-ECD DB-5 60 0.25 0.25 1 10

5 Pest. GC-ECD HP5-MS 30 0.25 0.25 4 2-1

PCB GC-ECD HP5-MS 30 0.25 0.25 4 0.5-

6 Pest.

PCB Autospec DB-5 55 0.25 0.1 5

0.

7 Pest. GC-ECD 5% phenyl methyl siloxane 50 0.2 0.33 6

e.g

0.

PCB GC-ECD 5% phenyl methyl siloxane 50 0.2 0.33 7

e.g. 

7

8 Pest. GC-MS 8 0,25

PCB GC-ECD 7 0,5 - 

9 Pest. HP 6890 GC/ECD DB-XLB/DB-5 60/60 0.25 0.25 5 2.5-

PCB HP 6890 GC/ECD DB-XLB/DB-5 60/60 0.25 0.25 5 2.5-
Summary of Methods Used

Lab No. Instrument Column Phase Length (m) Column i.d. (mm) Film Thickness (um) Points Co

10 Pest. GC-ECD DB-1 30 0.25 0.25 1 1

PCB GC-EI-MS DB-1 30 0.25 0.25 1 4

11 Pest. GC-ECD DB-5 30 0.25 0.25 5 5-2

PCB GC-ECD DB-5 30 0.25 0.25 5 5-

12 Pest. GC-ECD DB -1 30 0.25 0.25

PCB GC-MS DB-1 30 0.25 0.25 5 10-

13 Pest. GC-EIMS CPSil-8 50 0.25 0.12 7 2.5 - 

PCB GC-EIMS CPSil-8 50 0.25 0.12 7

1

14 Pest. PE ZB-5 30 0.25 0.25

PCB

15 Pest. GC db-5, db-608 30 0.53 1.5, 0.83 3 2 to 

PCB GC/MS DB-XLB 60 0.25 0.25 5 1 to 

16 Pest. GC-ECD DB5/DB17ht 60 0.25 250 6

2 

PCB GC-ECD DB5/DB17ht 60 0.25 250 6

2 

17 Pest. GC/MS DB-17MS 60 0.25 0.25 4 6n

PCB GC/MS DB-17MS 60 0.25 0.25 4 6n

18 Pest. GC-MS-MS DB 5 30 0.25 0.25

PCB GC-MS-MS DB 5 30 0.25 0.25
Summary of Methods Used

Lab Number

Method of 

Quantitation Added Prior to Extraction 

Indentity of Internal Standards 

Added Prior to Chromatographic 

Analysis

1 Pest. IS 4,4'-DDD-d 8, 4,4'-DDT-d 8, 13C-cis chlordane

PCB IS PCB 103 and PCB 198

2 Pest. IS PCB 103 and PCB 198 Phenanthrene-d10

PCB IS PCB 103 and PCB 198 Phenanthrene-d10

3 Pest. IS PCB 103 tetrachloro-o-xylene

PCB IS PCB 103 tetrachloro-o-xylene

4 Pest.

IS For SRM 1945; 

ES for Homog. V Ronnel Tetrachloro-m-xylene (TCMX)

PCB

IS For SRM 1945; 

ES for Homog. V 4,4' Dibromooctafluorobiphenyl (DOB), PCB 198 PCB 103

5 Pest. ES

PCB ES

6 Pest.

PCB IS

(ALL 13C-PCB):15, 77, 126, 169, 28, 118, 105, 156, 52, 

101, 128, 180, 194, 208, 209; and PCB 38-d 5

depending on fraction either 13CPCB 101 or 13C-PCB 111

7 Pest. IS PCB 155

PCB IS PCB 155

8 Pest. IS

PCB-198 (GC-MS); PCB-3 , PCB-40 , PCB-198 (GCECD)

13C-transchlordane (GC-MS); PCB53, PCB-55 PCB-207 (GC-ECD)

PCB IS PCB-3 , PCB-40 , PCB-198 PCB-53 , PCB-55 , PCB-207

9 Pest. IS

1-bromo-2-nitrobenzene (BNB), 

dibromooctofluorobiphenyl (DBOFB), 

octachloronaphthalene (OCN) BNB

PCB IS BNB,DBOFB, OCN BNB

10 Pest. ES

PCB ES

11 Pest. IS PCB 103 PCB 198, DBOFBP

PCB IS PCB 103 PCB 198, DBOFBP

12 Pest. ES

PCB IS 13C-28, 52, 101, 153, 138, 180, 209

13 Pest. IS 13C labelled PCBs: 28, 52, 101, 138, 153, 180 PCB 30, 13C labelled PCB 141

PCB IS 13C labelled PCBs: 28, 52, 101, 138, 153, 180 PCB 30, 13C labelled PCB 141

14 Pest.

PCB ES PCB 30 (Surrogate)

15 Pest. ES

PCB IS Chrysene-d 12

16 Pest. ES PCNB DOB

PCB ES PCB 103, 207, 209 DOB

17 Pest. IS 4,4'-DDD-d 8 and 4,4'-DDT-d 8

PCB IS PCB 103 and PCB 198

18 Pest. IS,ES

PCB IS,ES
Summary of Methods Used

Lab Number

Any Other Internal 

Standards? Added When?

IS Surrogate Standards Used 

for Quantitation Were Added:

If the IS/surrogates Added After 

Extraction/Cleanup, Were Results 

Corrected for Recovery?

1 Pest. Prior to extraction

PCB

2 Pest. Prior to extraction

PCB

3 Pest. tetrachloro-o-xylene Just prior to HPLC-SEC Prior to extraction

PCB tetrachloro-o-xylene No

4 Pest.

1,2,3-Trichlorobenzene 

(123-TCB) Before HPLC cleanup.

After extraction/cleanup prior to 

GC analysis

PCB PCB 192

5 Pest.

PCB

6 Pest. Prior to extraction Yes

PCB

7 Pest. PCB 155

Post analysis for samples 

requiring dilution

After extraction/cleanup prior to 

GC analysis No

PCB PCB 155

8 Pest.

After extraction/cleanup prior to 

GC analysis No

PCB

9 Pest. DBOFBP Before extraction

After extraction/cleanup prior to 

GC analysis No

PCB DBOFBP

10 Pest.

PCB

11 Pest. PCB 198, DBOFBP Before extraction Prior to extraction

PCB PCB 198, DBOFBP

12 Pest. Yes

PCB Added a 13C-105 

Just before GC-MS injection as 

an internal standard

13 Pest.

After extraction/cleanup prior to 

GC analysis No

PCB

14 Pest. No

PCB

15 Pest.

After extraction/cleanup prior to 

GC analysis No

PCB

16 Pest. Prior to extraction No

PCB

After extraction/cleanup prior to 

GC analysis.

17 Pest. Prior to extraction

PCB

18 Pest. 13C labeled DDE, DDT After extraction before cleanup

PCB

13C labeled PCB 52, 101, 

153, 138, 180
Summary of Methods Used

Lab Number Recovery Range (%)

Were PCBs 

Separated 

From Pesticides 

Prior to GC?

Does PCB 132 coelute 

with PCB 153 or with 

PCB 105 or is it 

separated from both?

Please Note Any Differences in Procedures Used for 

SRM 1945 Analyses from Those Used for Homog. V 

1 Pest. Yes

132 Coelutes with 153, 

Resolved from 105

PCB

2 Pest. No Unknown

PCB

3 Pest. 100-126 No

132 Coelutes with 153, 

Resolved from 105

PCB 100-126

4 Pest.

Ronnel: Homog. V (73.0 ± 4.1),

SRM1945 (107.4 ± 1.9%); 123-TCB:

Homog. V (67.8 ± 0.7), SRM1945

(101.1 ± 1.5) No

132 Coelutes with 153, 

Resolved from 105

After the column cleanup and lipid determination for each

SRM 1945 sample was done, the volume was adjusted to

10mL. Two extracts for each SRM sample was prepared

by taking 1mL of the sample and diluting it to 5mL. Final

sample cleanup of each sample portion was done by the

same procedure as for Homogenate V. The two portions of

HPLC cleaned extract for SRM 1945 sample 1 and 2 were

combined together during the concentration step to a final

volume of 1mL for GC analysis. For sample 3, only 1

portion was concentrated to 1mL.

PCB

DOB: Homog. V (67.8 ± 0.7),

SRM1945 (101.1 ± 1.5); PCB 198:

Homog. V (93.1 ± 2.0), SRM1945

(117.2 ± 11.2); PCB 192: Homog. V

(128.8 ± 2.5), SRM1945 (101.0 ± 

5 Pest. Yes Separates

PCB

6 Pest. 5-86 No

132 Coelutes with 153, 

Resolved from 105

PCB

7 Pest. Yes Coelutes with Both

PCB

8 Pest.

99.0 - 108.7 (GC-MS); 75.4 - 110.4 

(GC-ECD) No Separates

SRM 1945 was analysed with three replicates, while

Homogenate V was analysed with two replicates.

PCB 75.4 - 110.4

9 Pest. No

PCB 132 Was Not in 

Standard Mixture

The quantity of samples used for analysis are about three

times more for SRM 1945 than for Whale Blubber

Homegenate V.

PCB

10 Pest. Yes Separates

PCB

11 Pest. No

PCB

12 Pest. Yes

PCB 86 - 105

13 Pest. No

132 Coelutes with 153, 

Resolved from 105

PCB 67 - 91

14 Pest. Coelutes

PCB 75 - 105

15 Pest. Yes Separates

PCB

16 Pest. 58 - 69 Yes Separates

PCB 58 - 71

17 Pest. No Separates

PCB

18 Pest. No

132 Coelutes with 153, 

Resolved from 105

PCB
APPENDIX E 

Additional analyte data reported by individual laboratories.
Additional Analytical Results

Homog V Homog V Homog V SRM 1945 SRM 1945 SRM 1945

Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

Lab 1 Analyte (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt)

PCB 63 1.67 1.76 2.09 1.52 1.67 2.27

PCB 74 57.9 56.7 56.3 16.8 16.5 16.8

PCB 70+76 59.8 60.0 59.4 12.5 12.4 12.9

PCB 56+60 11.7 11.5 12.5 10.3 10.1 10.4

PCB 92+84+89 100 102 100 24.2 25.0 24.5

PCB 110 161 167 158 33.2 33.0 33.6

PCB 154 30.4 33.1 31.3 6.49 7.17 7.64

PCB 82 19.2 19.4 18.0 4.62 4.23 4.31

PCB 107 21.8 22.2 21.1 8.10 8.33 7.79

PCB 146 291 297 284 42.4 41.9 43.3

PCB 158 315 319 297 39.2 38.1 40.0

PCB 163 60.4 63.5 64.1 12.2 11.2 10.9

PCB 174 88.0 88.9 86.2 30.6 30.6 31.3

PCB 157 8.11 7.16 7.61 4.35 3.93 4.09

Lab 3 PCB 17 <1.57 <2.10 <1.87 2.05 <2.34 <1.69

PCB 70 <1.80 <2.41 <2.15 10.2 10.4 10.3

PCB 74 63.4 63.2 63.2 17.2 17.7 17.2

PCB 82 19.2 19.4 19.1 3.92 4.04 3.85

PCB 110 173 173 174 36.5 36.4 36

PCB 158 76.6 77.8 76.7 10.4 10.3 9.95

PCB 171 77.8 80.2 78.8 11.3 11.3 10.8

PCB 177 187 192 188 30.6 30.9 29.9

PCB 191 8.77 9.23 8.82 <2.01 <2.71 <1.95

PCB 199 219 226 219 88.1 88.3 83.8

PCB205 4.06 4.17 4.1 <2.01 <2.71 <1.96

PCB 208 38.8 39.8 38.8 24.6 24.7 23.7

Nonachlor III 139 137 137 28.2 28.6 27.9

Lab 4 Heptachlor 1.36 1.81 1.76 3.53 4.27 1.20

Aldrin 0.08 0.07 0.08 0.22 DL 0.45

Chlorpyrifos DL DL DL DL DL DL

Endrin 4.30 4.31 4.14 25.8 25.9 28.0

Endosulfan I OtherB OtherB OtherB OtherB OtherB OtherB

Endosulfan II 7.52 7.70 7.90 46.6 46.2 49.1

Endosulfan Sulfate 23.8 24.7 23.9 121 126 144

PCB 77 OtherD OtherD OtherD OtherD OtherD OtherD

PCB 110 OtherD OtherD OtherD OtherD OtherD OtherD

PCB 126 3.13 3.08 2.97 8.16 29.4 33.4

PCB 169 1.98 1.72 1.64 14.9 14.7 16.6

Other: We found that these pairs of analytes coelute from our DB-5 column.

OtherA : PCB 8 and alpha-HCH OtherB

 : endosulfan I and PCB 101 OtherC : alpha-chlordane and PCB 99.

OtherD

 : Since PCB 77and PCB 110 coelute from our column and the response of each congener vary from each other, the relative ratios

of two congeners can not be determined. We do not report the concentrations of either PCB 77 or PCB 110.

One replicate value for PCB 126 for SRM 1945 is questionable.

Lab 6 PBC 52 (+73) 167 171 172 40.6 41.5 39.3

PCB 87 (+115) 190 185 201 23.4 24.5 22.8

PCB 132 (+153) 3290 3830 3930 328 378 505

PCB 138 (+163+164+160)) 2080 2250 2300 225 250 297

PCB 187 (+182) 530 592 610 144 152 174

Lab 8 PCB 110 136 135 24.6 26.9 24.7

PCB 188 30.3 30.3 8.28 8.74 7.45

Lab 10 Tris(4-chlorophenyl) methane 152 148 159 1.29 1.21 1.32

Tris(4-chlorophenyl) methanol 149 140 153 1.60 1.54 1.60

PCDD/DFs and non-ortho coplanar PCBs in SRM1945

BRIEF DESCRIPTION OF PROCEDURES USED:

Mass of sample extracted: Homogenate V NA g, WET basis SRM 1945 4.5-4.8 g, WET basis

Method used for determining percentage lipid: Gravimetric determination from portion of the Soxhlet extraction

Were "wet" or "dry" samples extracted? Homogen, V NA SRM 1945 wet

Extraction method: Soxhlet

Extraction solvent: Mixture of hexane:dichloromethane (1:3)

Extraction time: 8 hours

Extraction - other: Sample was ground with anhydrous sodium sulfate. 
Additional Analytical Results

Sample extract cleanup method: A gel premeantion chromatography (GPC) (Gel: Bio-bead S-X3, Column: 2cm i.d. 50cm length)

After GPC, a silica-gel column was used for clean-up.

After Silica-gel column, an alumina and a carbon impressed silica-gel column were 

used for separation between PCBs and PCDD/DFs.

Analytical method used (e.g., GC-MS (include ionization type), GC-ECD):

Analyt. Instr. Column Phase Col. Length, m Col. i.d., mm Col. film thickness, μm

Non-ortho PCBs, Hp-OCDD/DFs GC-EI-HRMS DB-5ms 60 0.25 0.25

Te-HxCDD/DFs GC-EI-HRMS CP-Sil 88 60 0.25 0.1

Method of quantitation (IS = internal standard, ES = external standard):

DDs/DFs IS

Non-ortho PCBs IS

IF internal standard method was used, please complete the following section:

Identity of internal standards/surrogates used that were added PRIOR to extraction of sample:

PCDD/DFs (All 13C12-PCDD/DFs) 2378-TeCDD, 12378-PeCDD, 123678-HxCDD, 1234678-HpCDD, OCDD, 

2378-TeCDF, 12378-PeCDF, 123678-HxCDF, 123789-HxCDF, 1234678-HpCDF, OCDF

Non-ortho PCBs (All 13C12-PCBs) CB77,CB 81, CB126, CB169

 Added after extraction/cleanup and JUST PRIOR to chromatographic analysis:

PCDD/DFs (All 13C12-PCDDs) 1234-TeCDD and 123789-HxCDD

Non-ortho PCBs 13C12 1234-TeCDD

 Any others? Added at what point in analyses: no

PCDD/DFs ________________________________________________________________________

Non-ortho PCBs ________________________________________________________________________

 IS/surrogate standards used for quantitation calculations were: 

________ those added prior to extraction

yes those added after extraction/cleanup and just prior to chromatographic analysis

 If the IS/surrogates added after extraction/cleanup extraction were used for quantitation, 

 were results corrected for percent recovery? yes

 Percent recovery range:

PCDD/DFs 35-105%

Non-ortho PCBs 80-97%

Calibration Curve

Points Conc. Range

Te-PeCDD/DFs 5 0.4-16 ng/ml

Hx-HpCDD/DFs 5 0.8-32 ng/ml

OCDD/DF 5 1.6-64ng/ml

Non-ortho PCBs 5 2.5-100ng/ml

RESULTS:

Homog V Homog V Homog V SRM 1945 SRM 1945 SRM 1945

(percent) (percent) (percent) (percent) (percent) (percent)

Analyst (Initials) NA NA NA M.W. M.W. M.W.

Date(s) of measurements (m/d/y) NA NA NA 8/23-27/01 8/23-27/01 8/23-27/01

Homog V Homog V Homog V SRM 1945 SRM 1945 SRM 1945

PCDD/DFCongener Sample 1 Sample 2 Sample 3 Sample 1 Sample 2 Sample 3

(ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt) (ng/g wet wt)

2378-TeCDD NA NA NA 0.000569 0.000435 0.000443

12378-PeCDD NA NA NA 0.000584 <0.0005 <0.0005

123478-HxCDD NA NA NA <0.0005 <0.0005 <0.0005

123678-HxCDD NA NA NA 0.00117 0.00102 0.00112

123789-HxCDD NA NA NA <0.0005 <0.0005 <0.0005

1234678-HpCDD NA NA NA 0.00124 0.00116 0.00115

OCDD NA NA NA 0.00297 0.00242 0.00217

2378-TeCDF NA NA NA 0.00613 0.00577 0.00598

12378-PeCDF NA NA NA 0.000886 0.000687 0.000655

23478-PeCDF NA NA NA 0.00103 0.00106 0.00107

123478-HxCDF NA NA NA 0.000647 0.000555 0.000559

123678-HxCDF NA NA NA 0.000757 0.000681 0.000634

123789-HxCDF NA NA NA <0.0005 <0.0005 <0.0005

234678-HxCDF NA NA NA 0.00183 0.00152 0.00150

1234678-HpCDF NA NA NA 0.00105 0.000840 0.000834

1234789-HCDF NA NA NA <0.0005 <0.0005 <0.0005

OCDF NA NA NA <0.001 <0.001 <0.001

Additional Analytical Results

Non-ortho PCB congener

PCB 77 NA NA NA 0.381 0.368 0.369

PCB 81 NA NA NA 0.0576 0.0579 0.0556

PCB 126 NA NA NA 0.129 0.131 0.126

PCB 169 NA NA NA 0.117 0.116 0.117

Lab 13 PCB 74 71.0 58.0 67.0 18.0 21.0 18.0

PCB 110 200 170 200 38.0 46.0 40.0

PCB 114 8.00 7.30 7.99 2.10 2.59 2.28

PCB 141 28.0 27.0 28.0 16.0 19.0 17.0

PCB 157 21.0 21.0 21.0 7.61 9.54 8.77

PCB 158 100 120 105 10.0 12.0 11.0

PCB 167 13.0 11.0 10.0 9.20 12.0 9.72

PCB 174 95.0 86.0 99.0 24.0 27.0 25.0

PCB 199 11.0 10.0 11.0 3.84 4.36 3.88

PCB 203 210 200 220 72.0 73.0 68.0

Lab 17 PCB 45 & 49 108 112 103 22.7 23.4 23.2

PCB 74 55 56 51.9 14.4 14.3 14.7

PCB 70 <2 <2 <2 10.6 10.6 9.83

PCB 92 84.8 89.4 56 15.8 15.5 15.2

PCB 110 141 144 143 26.7 26.8 27.2

PCB 146 252 255 240 33.4 32.2 34.7

PCB 163 263 266 247 29.7 30.7 32.5

PCB 138 + 158 779 772 740 94.2 93.4 100

PCB 174 99.8 114 100 26.8 26.8 28.5

PCB 180+193 617 611 593 102 108 111

Lab 18 PCB 92 69.0 58.0 81.0 13.0 11.0 8.0

PCB 146 280 300 300 36.0 37.0 25.0

PCB 177 160 150 150 44.0 29.0 26.0

PCB 110 (+ 77) 170 160 180 29.0 41.0 29.0