NBS

PUBLICATIONS

A111Q3 ^S8b4

NBS SPECIAL PUBLICATION 260-1 04

U.S. DEPARTMENT OF COMMERCE/National Bureau of Standards

Standard Reference Materials:

Summary of the Biological

and Botanical Standards

Issued by the National

Bureau of Standards

qc R. Mavrodineanu and R. Alvarez

100

• U57

260-104

C 2

1985
TM he National Bureau of Standards' was established by an act of Congress on March 3, 1901. The

U Bureau's overall goal is to strengthen and advance the nation's science and technology and facilitate their effective application for public benefit. To this end, the Bureau conducts research and provides: (1) a

basis for the nation's physical measurement system, (2) scientific and technological services for industry and

government, (3) a technical basis for equity in trade, and (4) technical services to promote public safety.

The Bureau's technical work is performed by the National Measurement Laboratory, the National

Engineering Laboratory, the Institute for Computer Sciences and Technology, and the Institute for Materials

Science and Engineering

.

The National Measurement Laboratory

Provides the national system of physical and chemical measurement;

coordinates the system with measurement systems of other nations and

furnishes essential services leading to accurate and uniform physical and

chemical measurement throughout the Nation's scientific community, in- dustry, and commerce; provides advisory and research services to other Government agencies; conducts physical and chemical research; develops,

produces, and distributes Standard Reference Materials; and provides

calibration services. The Laboratory consists of the following centers:

• Basic Standards2 • Radiation Research

• Chemical Physics

• Analytical Chemistry

The National Engineering Laboratory

Provides technology and technical services to the public and private sectors to address national needs and to solve national problems; conducts research in engineering and applied science in support of these efforts; builds and maintains competence in the necessary disciplines required to carry out this research and technical service; develops engineering data and measurement

capabilities; provides engineering measurement traceability services; develops

test methods and proposes engineering standards and code changes; develops

and proposes new engineering practices; and develops and improves

mechanisms to transfer results of its research to the ultimate user. The

Laboratory consists of the following centers:

Applied Mathematics

Electronics and Electrical Engineering2 Manufacturing Engineering

Building Technology

Fire Research

Chemical Engineering2

The Institute for Computer Sciences and Technology

Conducts research and provides scientific and technical services to aid Federal agencies in the selection, acquisition, application, and use of computer technology to improve effectiveness and economy in Government

operations in accordance with Public Law 89-306 (40 U.S.C. 759), relevant Executive Orders, and other directives; carries out this mission by managing

the Federal Information Processing Standards Program, developing Federal ADP standards guidelines, and managing Federal participation in ADP

voluntary standardization activities; provides scientific and technological advisory services and assistance to Federal agencies; and provides the technical foundation for computer-related policies of the Federal Government. The Institute consists of the following centers:

Programming Science and

Technology

Computer Systems

Engineering

The Institute for Materials Science and Engineering

Conducts research and provides measurements, data, standards, reference

materials, quantitative understanding and other technical information fundamental to the processing, structure, properties and performance of materials; addresses the scientific basis for new advanced materials technologies; plans

research around cross-country scientific themes such as nondestructive

evaluation and phase diagram development; oversees Bureau-wide technical programs in nuclear reactor radiation research and nondestructive evaluation; and broadly disseminates generic technical information resulting from

its programs. The Institute consists of the following Divisions:

Ceramics

Fracture and Deformation 3 Polymers

Metallurgy

Reactor Radiation

'Headquarters and Laboratories at Gaithersburg, MD, unless otherwise noted; mailing address Gaithersburg, MD 20899.

: Some divisions within the center are located at Boulder, CO 80303. 'Located at Boulder, CO, with some elements at Gaithersburg, MD.
Standard Reference Materials:

Summary of the Biological and

Botanical Standards Issued by the

National Bureau of Standards

R. Mavrodineanu 1

Center for Analytical Chemistry

National Measurement Laboratory

National Bureau of Standards

Gaithersburg, MD 20899

and

R. Alvarez

Office of Standard Reference Materials

National Measurement Laboratory

National Bureau of Standards

Gaithersburg, MD 20899

1 Present Address: 227 Almeria Road

West Palm Beach, FL 33405

z

8<

Q

U.S. DEPARTMENT OF COMMERCE, Malcolm Baldrige, Secretary

NATIONAL BUREAU OF STANDARDS, Ernest Ambler, Director

Issued October 1985
Library of Congress Catalog Card Number: 85-600605

National Bureau of Standards Special Publication 260-104

Natl. Bur. Stand. (U.S.), Spec. Publ. 260-104, 68 pages (Oct. 1985)

CODEN: XNBSAV

U.S. GOVERNMENT PRINTING OFFICE

WASHINGTON: 1985

For sale by the Superintendent of Documents, U.S. Government Printing Office, Washington, DC 20402
Preface

Standard Reference Materials (SRM's) as defined by the National Bureau of Standards (NBS) are well-characterized materials, produced in quantity and certified for one or more physical or chemical properties. They are used to assure the accuracy and compatibility of measurements

throughout the Nation. SRM's are widely used as primary standards in many diverse fields in science, industry, and technology, both within the United States and throughout the world. They are also used extensively in the fields of environmental and clinical analysis. In many applications,

traceabi lify of quality control and measurement processes to the national measurement system is carried out through the mechanism and use of SRM's. For many of the Nation's scientists and technologists it is therefore of more than passing interest to know the details of the measurements

made at NBS in arriving at the certified values of the SRM's produced. An NBS series of papers, of which this publication is a member, called the NBS Special Publication - 260 Series , is reserved for this purpose.

This 2o0 Series is dedicated to the dissemination of information on different phases of the preparation, measurement, certification and use of NBS SRM's. In general, much more detail will be found in these papers than is generally allowed, or desirable, in scientific journal articles. This

enables the user to assess the validity and accuracy of the measurement processes employed, to judge

the statistical analysis, and to learn details of techniques and methods utilized for work entailing

the greatest care and accuracy. These papers also should provide sufficient additional information

not found on the certificate so that new applications in diverse fields not foreseen at the time the SRM was originally issued will be sought and found.

Inquiries concerning the technical content of this paper should be directed to the author(s).

Other questions concered with the availability, delivery, price, and so forth, will receive prompt

attention from:

Office of Standard Reference Materials

Nat iornl Bureau of Standards

Gaithersburg, MI) 20899

Stanley D. Rasberry, Chief

Office of Standard Reference Materials

iii
OTHER NBS PUBLICATIONS IN THIS SERIES

Catalog of NBS Standard Reference Materials

(1984-85 edition), Catherine H. Hudson, ed., NBS

Spec. Publ. 260 (February 1984). $5.50* SN003-

003-02558-5.

Michaelis, R. E., and Wyman, L. L. Standard

Reference Materials: Preparation of White Cast

Iron Spectrochemical Standards. NBS Misc. Publ. 260-1 (June 1964). COM 74- 1 106 1 **

Michaelis, R. E., Wyman, L. L., and Flitsch, R., Standard Reference Materials: Preparation of NBS Copper-Base Spectrochemical Standards. NBS Misc. Publ. 260-2 (October 1964). COM74-

11063**

Michaelis, R. E., Yakowitz, H., and Moore, G. A., Standard Reference Materials: Metallographic

Characterization of an NBS Spectrometric LowAlloy Steel Standard. NBS Misc. Publ. 260-3 (October 1964). COM74-1 1060** Hague, J. L. Mears, T. W., and Michaelis, R. E., Standard Reference Materials: Sources of

Information, NBS Misc. Publ. 260-4 (February

1965). COM74-11059

Alvarez, R., and Flitsch R., Standard Reference

Materials: Accuracy of Solution X-Ray Spectrometric Analysis of Copper-Base Alloys. NBS

Misc. Publ. 260-5 (March 1965). PB168068**

Shultz, J. I., Standard Reference Materials: Methods for the Chemical Analysis of White

Cast Iron Standards, NBS Misc. Publ. 260-6

(July 1965). COM74-11068**

Bell, R. K., Standard Reference Materials: Methods for the Chemical Analysis of NBS

Copper-Base Spectrochemical Standards. NBS

Misc. Publ. 260-7 (October 1965). COM74- 11067** Richmond, M.S., Standard Reference Materials: Analysis of Uranium Concentrates at the

National Bureau of Standards. NBS Misc. Publ. 260-8 (December 1965). COM74-1 1066** Anspach, S. C, Cavallo, L. M. Garfinkel, S. B. Hutchinson, J. M. R., and Smith, C. N., Standard Reference Materials: Half Lives of Materials Used in the Preparation of Standard Reference Materials of Nineteen Radioactive Nuclides

Issued by the National Bureau of Standards NBS Misc. Publ. 260-9 (November 1965). COM74-I1065**

Yakowitz. H., Vieth, D. L., Heinrich, K. F. J., and

Michaelis, R. E., Standard Reference Materials: Homogeneity Characterization of NBS Spectrometric Standards II: Cartridge Brass and LowAlloy Steel, NBS Misc. Publ. 260-10 (December

(1965). COM74-11064**

Napolitano, A., and Hawkins, E. G., Standard Reference Materials: Viscosity of Standard

Lead-Silica Glass, NBS Misc. Publ. 260-11 (November 1966). NBS Misc. Publ. 260-11** Yakowitz, H., Vieth, D. L., and Michaelis, R. E., Standard Reference Materials: Homogeneity

Characterization of NBS Spectrometric Standards III: White Cast Iron and Stainless Steel Powder Compact, NBS Misc. Publ. 260-12

(September 1966). NBS Misc. Publ. 260-12**

Spijkerman, J. L., Snediker, D. K.., Ruegg, F. C,

and DeVoe, J. R., Standard Reference Materials: Mossbauer Spectroscopy Standard for the Chemical Shift of Iron Compounds, NBS Misc. Publ. 260-13 (July 1967). NBS Misc. Publ.

260-13**

Menis, O., and Sterling, J. T., Standard Reference

Materials: Determination of Oxygen in Ferrous

Materials - SRM 1090, 1091, and 1092, NBS

Misc. Publ. 260-14 (September 1966). NBS Misc.

Publ. 260-14**

Passaglia, E., and Shouse, P. J., Standard Refer- ence Materials: Recommended Method of Use

of Standard Light-Sensitive Paper for Calibrat- ing Carbon Arcs Used in Testing Textiles for Colorfastness to Light, NBS Misc. Publ. 260-15 (June 1967). (Replaced by NBS Spec. Publ.

260-41.) Yakowitz, H., Michaelis, R. E., and Vieth, D. L., Standard Reference Materials: Homogeneity

Characterization of NBS Spectrometric Standards IV: Preparation and Microprobe Characterization of W-20% MO Alloy Fabricated by

Powder Metallurgical Methods, NBS Spec.

Publ. 260-16 (January 1969). COM74-1 1062** Catanzaro, E. J., Champion, C. E., Garner, E. L., Marinenko, G., Sappenfield, K. M., and Shields, W. R., Standard Reference Materials: Boric

Acid; Isotopic and Assay Standard Reference

Materials, NBS Spec. Publ. 260-17 (February

_1970). Out of Print. Geller, S. B., Mantek, P.A., and Cleveland, N.

G., Standard Reference Materials: Calibration of NBS Secondary Standard Magnetic Tape

(Computer Amplitude Reference) Using the Reference Tape Amplitude Measurement

"Process A," NBS Spec. Publ. 260-18 (November

1969). (See NBS Spec. Publ. 260-29.) Paule, R. C, and Mandel, J., Standard Reference

Materials: Analysis of Interlaboratory Measurements on the Vapor Pressure of Gold (Certifica- tion of Standard Reference Material 745). NBS

Spec. Publ. 260-19 (January 1970). PB190071**

Paule, R. C, and Mandel, J., Standard Reference

Materials: Analysis of Interlaboratory Measure- ments on the Vapor Pressures of Cadmium and

Silver, NBS Spec. Publ. 260-21 (January 1971). COM74- 1 1359** Yakowitz, H., Fiori, C. E., and Michaelis, R. E., Standard Reference Materials: Homogeneity

Characterization of Fe-3 Si Alloy, NBS Spec.

Publ. 260-22 (February 1971). COM74-1 1357**

Napolitano, A., and Hawkins, E. G., Standard

Reference Materials: Viscosity of a Standard

Borosilicate Glass, NBS Spec. Publ. 260-23 (December 1970). COM7I-00157**

Sappenfield, K. M., Marineko. G.. and Hague, J. L., Standard Reference Materials: Comparison

of Redox Standards, NBS Spec. Publ. 260-24 (January 1972). COM72-50058**

iv
Hicho, G. E., Yakowitz, H., Rasberry, S. D., and

Michaelis, R. E., Standard Reference Materials: A Standard Reference Material Containing

Nominally Four Percent Austenite, NBS Spec.

Publ. 260-25 (February 1971). COM74-1 1356** Martin, J. F., Standard Reference Materials:

National Bureau of Standards-US Steel Corporation Joint Program for Determining Oxygen and Nitrogen in Steel, NBS Spec. Publ. 260-26

(February 1971). 85 cents* PB 81 176620

Garner, E. L., Machlan, L. A., and Shields, W. R., Standard Reference Materials: Uranium

Isotopic Standard Reference Materials, NBS

Spec. Publ. 260-27 (April 1971). COM74-

1 1358**

Heinrich, K. F. J., Myklebust, R. L., Rasberry, S. D., and Michaelis, R. E., Standard Reference Materials: Preparation and Evaluation of SRM's 481 and 482 Gold-Silver and GoldCopper Alloys for Microanalysis, NBS Spec. Publ. 260-28 (August 1971). COM71-50365**

Geller, S. B , Standard Reference Materials: Cali- bration of NBS Secondary Standard Magnetic

Tape (Computer Amplitude Reference) Using

the Reference Tape Amplitude Measurement

-Process A-Model 2," NBS Spec. Publ. 260-29 (June 1971). COM71-50282

Gorozhanina, R. S., Freedman, A. Y., and

Shaievitch, A. B. (translated by M. C. Selby), Standard Reference Materials: Standard

Samples Issued in the USSR (A Translation from the Russian). NBS Spec. Publ. 260-30 (June

1971). COM71-50283**

Hust, J. G., and Sparks, L. L., Standard Reference

Materials: Thermal Conductivity of Electrolytic Iron SRM 734 from 4 to 300 K, NBS Spec. Publ. 260-31 (November 1971). COM71-50563**

Mavrodineanu, R., and Lazar, J. W., Standard

Reference Materials: Standard Quartz Cuvettes, for High Accuracy Spectrophotometry, NBS

Spec. Publ. 260-32 (December 1973). 55 cents* SN003-003-012I3-1 Wagner, H. L., Standard Reference Materials: Comparison of Original and Supplemental

SRM 705, Narrow Molecular Weight Distri- bution Polystyrene, NBS Spec. Publ. 260-33 (May 1972). COM72-50526**

Sparks, L. L., and Hust, J. G., Standard Reference

Materials: Thermoelectric Voltage, NBS Spec.

Publ. 260-34, (April 1972). COM72-5037I**

Sparks, L. L., and Hust, J. G., Standard Refer- ence Materials: Thermal Conductivity of

Austenitic Stainless Steel, SRM 735 from 5 to 280 K, NBS Spec. Publ. 260-35 (April 1972.) 35 cents* COM72-50368**

Cali, J. P., Mandel, J., Moore, L. J., and Young, D

S., Standard Reference Materials: A Referee Method for the Determination of Calcium in Serum, NBS SRM 915, NBS Spec. Publ. 260-36 (May 1972). COM72-50527**

Shultz, J. I. Bell., R. K. Rains, T. C, and Menis,

O., Standard Reference Materials: Methods of

Analysis of NBS Clay Standards, NBS Spec.

Publ. 260-37 (June 1972). COM72-50692**

Richmond, J. C, and Hsia, J. J., Standard Refer- ence Materials: Preparation and Calibration of Standards of Spectral Specular Reflectance, NBS Spec. Publ. 260-38 (May 1972). COM72-

50528**

Clark, A. F., Denson, V.A., Hust, J. G., and

Powell, R. L., Standard Reference Materials: The Eddy Current Decay Method for Resistivity Characterization of High-Purity Metals, NBS

Spec. Publ. 260-39 (May 1972). COM72-50529**

McAdie, H. G., Garn, P.D .and Menis, O., Standard Reference Materials: Selection of Thermal

Analysis Temperature Standards Through a Cooperative Study (SRM 758, 759, 760), NBS

Spec. Publ. 260-40 (August 1972.) COM72-

50776** Wood, L. A., and Shouse, P. J., Standard

Reference Materials: Use of Standard LightSensitive Paper for Calibrating Carbon Arcs Used in Testing Textiles for Colorfastness to Light, NBS Spec. Publ. 260-41 (August 1972) COM72-50775**

Wagner, H. L. and Verdier, P. H., eds.. Standard

Reference Materials: The Characterization of Linear Polyethylene, SRM 1475, NBS Spec. Publ. 260-42 (September 1972). COM72-

50944**

Yakowitz, H., Ruff, A. W., and Michaelis, R. E., Standard Reference Materials: Preparation and Homogeneity Characterization of an Austenitic Iron-Chromium-Nickel Alloy, NBS Spec. Publ. 260-43 (November 1972). COM73-50760**

Schooley, J. F., Soulen, R. J., Jr., and Evans, G. A.,

Jr., Standard Reference Materials: Preparation and Use of Superconductive Fixed Point

Devices, SRM 767, NBS Spec. Publ. 260-44 (December 1972). COM73-50037**

Greifer, B., Maienthal, E. J., Rains, T. C, and

Rasberry, S. D., Standard Reference Materials: Powdered Lead-Based Paint, SRM 1579, NBS

Spec. Publ. 260-45 (March 1973). COM73-

50226**

Hust, J. G., and Giarratano, P. J., Standard Refer- ence Materials: Thermal Conductivity and

Electrical Resistivity Standard Reference Materials: Austenitic Stainless Steel, SRM's 735 and

798, from 4 to 1200 K, NBS Spec. Publ. 260-46 (March 1975). SN003-003-0I278-5*

Hust, J. G., Standard Reference Materials: Electrical Resistivity of Electrolytic Iron, SRM 797, and Austenitic Stainless Steel, SRM 798, from 5 to 280 K, NBS Spec. Publ. 260-47 (February

1974). COM74-50I76** Mangum, B. W., and Wise, J. A., Standard Refer- ence Materials: Description and Use of Precision Thermometers for the Clinical Laboratory,

SRM 933 and SRM 934. NBS Spec. Publ. 260-48 (May 1974). 60 cents* SN003-003-01 278-5 Carpenter, B. S., and Reimer, G. M., Standard

Reference Materials: Calibrated Glass Standards for Fission Track Use, NBS Spec. Publ. 260-49 (November 1974). COM74-5I185

v
Hust. J. G.. and Giarratano, P. J., Standard Refer- ence Materials: Thermal Conductivity and Electrical Resistivity Standard Reference Materials:

Electrolytic Iron, SRM's 734 and 797 from 4 to 1000 K, NBS Spec. Publ. 260-50 (June 1975). $1.00* SN003-003-0 1425-7 Mavrodineanu, R., and Baldwin, J. R., Standard

Reference Materials: Glass Filters As a Standard Reference Material for Spectrophotometry;

Selection; Preparation; Certification; Use-SRM

930, NBS Spec. Publ. 260-51 (November 1975). $1.90* SN003-003-0148I-8

Hust, J. G., and Giarratano, P. J., Standard Refer- ence Materials: Thermal Conductivity and Electrical Resistivity Standard Reference Materials 730 and 799, from 4 to 3000 K, NBS Spec. Publ. 260-52 (September 1975). $1.05* SN003-003-

01464-8 Durst, R. A., Standard Reference Materials: Standardization of pH Measurements. NBS

Spec. Publ. 260-53 (December 1975, Revised). $1.05 SN003-003-01551-2

Burke, R. W., and Mavrodineanu, R., Standard

Reference Materials: Certification and Use of Acidic Potassium Dichromate Solutions as an

Ultraviolet Absorbance Standard, NBS Spec.

Publ. 260-54 (August 1977). $3.00* SN003-003-

01828-7

Ditmars, D. A., Cezairliyan, A., lshihara, S., and

Douglas, T. B., Standard Reference Materials: Enthalpy and Heat Capacity; Molybdenum

SRM 781, from 273 to 2800 K, NBS Spec. Publ. 260-55 (September 1977). $2.20* SN003-003-

01836-8

Powell, R. L., Sparks, L. L., and Hust, J. G., Standard Reference Materials: Standard

Thermocouple Materials, Pt.67: SRM 1967, NBS

Spec. Publ. 260-56 (February 1978). $2.20* SN003-003-0I8864

Cali, J. P. and Plebanski, T., Guide to United

States Reference Materials, NBS Spec. Publ. 260-57 (February 1978). $2.20* PB 277173

Barnes, J. D., and Martin, G. M., Standard Refer- ence Materials: Polyester Film for Oxygen Gas

Transmission Measurements SRM 1470, NBS

Spec. Publ. 260-58 (June 1979) $2.00* SN003-

003-02077 Chang, T., and Kahn, A. H. Standard Reference

Materials: Electron Paramagnetic Resonance

Intensity Standard; SRM 2601, NBS Spec.

Publ. 260-59 (August 1978) $2.30* SN003-

003-01975-5 Velapoldi, R. A., Paule, R. C, Schaffer, R., Mandel, J., and Moody, J. R., Standard Refer- ence Materials: A Reference Method for the Determination of Sodium in Serum, NBS Spec.

Publ. 260-60 (August 1978). $3.00* SN003-003

01978-0

Verdier, P. H., and Wagner. H. L., Standard Refer- ence Materials: The Characterization of Linear Polyethylene (SRM 1482, 1483, 1484), NBS

Spec. Publ. 260-61 (December 1978). $1.70* SN003-003-02006-1

Soulen, R. J., and Dove, R. B., Standard Reference

Materials: Temperature Reference Standard

for Use Below 0.5 K (SRM 768). NBS Spec.

Publ. 260-62 (April 1979). $2.30* SN003-003-

02047-8 Velapoldi, R. A., Paule, R. C, Schaffer, R. Mandel, J., Machlan, L. A., and Gramlich, J. W., Standard Reference Materials: A Reference Method for the Determination of Potassium in Serum. NBS Spec. Publ. 260-63 (May 1979). $3.75* SN003-003-02068

Velapoldi, R. A., and Mielenz, K. D., Standard

Reference Materials: A Fluorescence Standard

Reference Material Quinine Sulfate Dihydrate

(SRM 936), NBS Spec. Publ. 260-64 (January

1980). $4.25* SNO03-OO3-02 148-2 Marinenko, R. B., Heinrich, K. F. J., and Ruegg,

F. C, Standard Reference Materials: Micro- Homogeneity Studies of NBS Standard Reference

Materials, NBS Research Materials, and Other Related Samples. NBS Spec. Publ. 260-65

(September 1979). $3.50* SN003-003-021 14-1 Venable, W. H., Jr., and Eckerle, K L., Standard

Reference Materials: Didymium Glass Filters for Calibrating the Wavelength Scale of Spectro- photometers (SRM 2009, 2010, 2013). NBS Spec.

Publ. 260-66 (October 1979). $3.50* SN003-003-

02127-0

Velapoldi, R. A., Paule, R. C, Schaffer, R., Mandel,

J., Murphy, T. J., and Gramlich, J. W., Standard

Reference Materials: A Reference Method for the Determination of Chloride in Serum, NBS Spec.

Publ. 260-67 (November 1979). $3.75* SN003-

003-02 1 36-9 Mavrodineanu, R. and Baldwin, J. R., Standard

Reference Materials: Metal-On-Quartz Filters as a Standard Reference Material for Spectrophotometry-SRM 2031, NBS Spec. Publ. 260-68

(April 1980). $4.25* SN003-003-02 167-9 Velapoldi, R. A., Paule, R. C, Schaffer, R., Mandel, J., Machlan, L. A., Garner, E. L., and

Rains, T. C, Standard Reference Materials: A

Reference Method for the Determination of Lithium in Serum, NBS Spec. Publ. 260-69 (July)

1980). $4.25* SN003-003-02214-4 Marinenko, R. B., Biancaniello, F., Boyer, P. A., Ruff, A. W., DeRobertis, L., Standard Reference

Materials: Preparation and Characterization of an Iron-Chromium-Nickel Alloy for Microanalysis, NBS Spec. Publ. 260-70 (May 1981). $2.50* SN003-003-02328-1 Seward, R. W., and Mavrodineanu, R., Standard

Reference Materials: Summary of the Clinical Laboratory Standards Issued by the National Bureau of Standards, NBS Spec. Publ. 260-71 (November 1981). $6.50* SN003-003-02381-7

Reeder, D.J., Coxon, B., Enagonio, D., Christensen,

R. G., Schaffer, R., Howell, B. F., Paule, R. C,

Mandel, J., Standard Reference Materials: SRM

900, Antiepilepsy Drug Level Assay Standard, NBS Spec. Publ. 260-72 (June 1981). $4.25* SN003-003-02329-9

vi
Interrantc, C. G., and Hicho, G. E., Standard Refer- ence Materials: A Standard Reference Material Containing Nominally Fifteen Percent Austenite (SRM 486), NBS Spec. Publ. 260-73 (January

1982). $2.75* SN003-003-02386-8 Marinenko, R. B., Standard Reference Materials: Preparation and Characterization of K-4I1 and

K-4I4 Mineral Glasses for Microanalysis: SRM

470. NBS Spec. Publ. 260-74 (April 1982). $3.50 SN003-003-023-95-7

Weidner, V. R., and Hsia, J. J., Standard Reference

Materials: Preparation and Calibration of First Surface Aluminum Mirror Specular Reflectance Standards (SRM 2003a), NBS Spec. Publ. 260-75 (May 1982). $3.75 SN003-003-023-99-0

Hicho, G. E. and Eaton, E. E., Standard Reference Materials: A Standard Reference Material

Containing Nominally Five Percent Austenite (SRM 485a), NBS Spec. Publ. 260-76 (August

1982). $3.50 SN003-003-024-33-3 Furukawa, G. T„ Riddle, J. L., Bigge, W. G., and

Pfieffer, E. R., Standard Reference Materials: Application of Some Metal SRM's as Thermometric Fixed Points, NBS Spec. Publ. 260-77 (August 1982). $6.00 SN003-003-024-34-I

Hicho, G. E. and Eaton, E. E., Standard Reference

Materials: Standard Reference Material Containing Nominally Thirty Percent Austenite (SRM

487), NBS Spec. Publ. 260-78 (September 1982). $3.75* SN003-003-024-35-0 Richmond, J. C, Hsia, J. J. Weidner. V. R., and

Wilmering, D. B., Standard Reference Materials: Second Surface Mirror Standards of Specular

Spectral Reflectance (SRM's 2023. 2024, 2025), NBS Spec. Publ. 260-79 (October 1982). $4.50* SN003-003-024-47-3

Schaffer, R.. Mandel, J., Sun, T., Cohen. A., and

Hertz, H. S., Standard Reference Materials: Evaluation by an ID/ MS Method of the AACC

Reference Method for Serum Glucose, NBS

Spec. Publ. 260-80 (October 1982). $4.75* SN003-

003-024-43-1 Burke, R. W., and Mavrodincanu, R. (NBS retired), Standard Reference Materials: Accuracy in Analytical Spectrophotometry, NBS Spec. Publ. 260-81 (April 1983). $6.00* SN003-003-024-8

Weidner, V. R., Standard Reference Materials: White Opal Glass Diffuse Spectral Reflectance Standards for the Visible Spectrum (SRM's 2015 and 2016). NBS Spec. Publ. 260-82 (April 1983). $3.75* SN003-003-024-89-9**

Bowers, G. N., Jr., Alvarez, R., Cali, J. P. (NBS

retired). Eberhardt, K. R., Reeder, D. J., Schaffer, R., Uriano, G. A , Standard Reference

Materials: The Measurement of the Catalytic

(Activity) Concentration of Seven Enzymes in NBS Human Serum SRM 909, NBS Spec.

Publ. 260-83 (June 1983). $4.50* SN003-003-024-

99-6

Gills, T. E., Seward, R. W., Collins, R. J., and

Webster, W. C, Standard Reference Materials: Sampling. Materials Handling. Processing, and

Packaging of NBS Sulfur in Coal Standard

Reference Materials, 2682, 2683, 2684, and 2685, NBS Spec. Publ. 260-84 (August 1983). $4.50* SN003-003-025-20-8

Swyt, D. A., Standard Reference Materials: A Look

at Techniques for the Dimensional Calibration of Standard Microscopic Particles, NBS Spec. Publ. 260-85 (September 1983). $5.50* SN003-003-

025-21-6

Hicho, G. E. and Eaton, E. E., Standard Reference Materials: A Standard Reference Material

Containing Two and One-Half Percent Austenite, SRM 488, NBS Spec. Publ. 260-86 (December

1983). $1.75* SN003-003-025-4I-1 Mangum, B. W., Standard Reference Materials: SRM 1969: Rubidium Triple-Point - A Temperature Reference Standard Near 39.30 °C, NBS

Spec. Publ. 260-87 (December 1983). $2.25* SN003-003-025-44-5 Gladney, E. S.. Burns, C. E., Perrin, D. R., Roelandts, I., and Gills, T. E., Standard Reference Materials: 1982 Compilation of Elemental

Concentration Data for NBS Biological, Geological, and Environmental Standard Reference

Materials. Spec. Publ. 260-88 (March 1984). $7.00* SN003-003-02565-8

Hust, J. G., Standard Reference Materials: A Fine- Grained, Isotropic Graphite for Use as NBS

Thermophysical Property RM's from 5 to 2500 K, NBS Spec. Publ. 260-89 (September 1984). $4.50* SN003-003-02608-5 Hust, J. G., and Lankford, A. B.„ Standard

Reference Materials: Update of Thermal

Conductivity and Electrical Resistivity of

Electrolytic Iron, Tungsten, and Stainless Steel, NBS Spec. Publ. 260-90 (September 1984). $3.00* SN003-003-02609-3

Goodrich, L. F., Vecchia, D. F., Pittman, E. S., Ekin, J. W.. and Clark, A. F., Standard Reference

Materials: Critical Current Measurements on an NbTi Superconducting Wire Standard Reference

Material, NBS Spec. Publ. 260-91 (September

1984). $2.75* SN003-003-02614-0

Carpenter, B. S., Standard Reference Materials: Calibrated Glass Standards for Fission Track Use (Supplement to NBS Spec. 260-49). NBS

Spec. Publ.. 260-92 (September 1984). $1.50* SN003-003-02610-7

Ehrstein, J., Preparation and Certification of Standard Reference Materials for Calibration of Spreading Resistance Probes, NBS Spec. Publ. 260-93 (January 1985). $2.25* SN003-003-02633-6

Gills. T. E., Koch, W. F. Stolz, J. W., Kelly, W. R., Paulsen, P. J., Colbert, J. C. Kirklin, D. R., Pei, P.T.S., Weeks, S., Lindstrom, R. M.

Fleming, R. F., Greenberg, R R., and

Paule, R. C, Methods and Procedures Used at the National Bureau of Standards to Certify Sulfur

in Coal SRM's for Sulfur Content, Calorific Value, Ash Content, NBS Spec. Publ. 260-94 (December 1984). $2.25* SN003-003-02629-8

Mulholland, G. W„ Hartman, A. W., Hembree,

G. G., Marx, E., and Lettieri, T. R., Standard

Reference Materials: Development of a 1 m

Diameter Particle Size Standard, SRM 1690, NBS Spec. Publ. 260-95 (May 1985). SN0O3-OO3-

02665-4

Carpenter, B. S„ Gramlich, J. W., Greenberg, R. R., and Machlan, L. A., Standard Reference

Materials: Uranium-235 Isotopic Abundance Standard Reference Materials for Gamma

Spectrometry Measurements, NBS Spec. Publ. 260-96 (In Preparation).

vii
Mavrodineanu, R. and Gills, T. E., Standard

Reference Materials: Summary of the Coal, Ore,

Mineral, Rock, and Refractory Standards Issued by the National Bureau of Standards, NBS Spec.

Publ. 260-97 (September 1985). Hust, J. G , Standard Reference Materials: Glass Fiberboard SRM for Thermal Resistance, NBS

Spec Publ. 260-98 (August 1985). SN0O3-003-

02674-3

Callanan, J. E., Sullivan, S. A., and Vecchia, D. F., Standard Reference Materials: Feasibility Study

for the Development of Standards Using

Differential Scanning Calorimetry, NBS Spec.

Publ. 260-99 (August 1985). SN003-003-02675-

1

Taylor, J. K., Standard Reference Materials: Hand- book for SRM Users, NBS Spec. Publ. 260-100

(In Press). Mangum, B. W., Standard Reference Materials: SRM 1970, Succinonitrile Triple-Point Standard: A Temperature Reference Standard Near

58.08 °C, NBS Spec. Publ. 260-101 (In

Preparation). Mavrodineanu, R., Standard Reference Materials: Holmium Oxide Solution Wavelength Standard from 240- 650 nm, SRM 2034, NBS Spec. Publ. 260-102 (In Press). Hust, J. G., Standard Reference Materials: Glass Fiberblanket SRM for Thermal Resistance, NBS

Spec. Publ. 260-103 (September 1985). SN003-

003-02687-5 Mavrodineanu, R. and Alvarez, R., Standard

Reference Materials: Summary of the Biological and Botanical Standards Issued by the National Bureau of Standards, NBS Spec. Publ. 260-104

(In Press).

* Send order with remittance to Superintendent

of Documents, US Government Printing Office Washington, DC 20402. Remittance from

foreign countries should include an additional one-fourth of the purchase price for postage. •* May be ordered from: National Technical Information Services (NTIS). Springfield

Virginia 22161.
TABLE OF CONTENTS

Page

Preface iii

Abstract x

Introduction 1

Table 1. Categories of Standard Reference Materials available from the National Bureau of Standards 2

Biological and Botanical Standards 4

Table 2. Summary of the Biological and Botanical Standards 6

Table 3. Summary of the Analytical Data Obtained for the Biological and Botanical Standard Reference Materials and Research Material 12

Appendix I. Alphabetical Index by Standard Reference Material Name 14

Appendix II. Certificates for Biological and Botanical Standards

(listed in numerical order) 27

ix
Abs tract

This publication is a summary of the biological and botanical Standard Reference Materials and Research Materials issued by the National Bureau of Standards. The material, composition, certification, use, and remarks

concerning each of the ten materials described are pres- ented in tabular form. Copies of the Certificates of Analysis for these materials are contained in the appendix

for more detailed information.

Key Words: Biological materials; botanical materials;

chemical compositions; Research Material;

Standard Reference Material.

x
Introduction

Since its inauguration in 1901, the National Bureau of Standards (NBS) has issued nearly 2000 different Standard Reference Materials (SRM's). Many of these have been renewed several times, many have been replaced or discontinued as technology changed. Today, over 900 SRM's are available,

together with a large number of scientific publications related to the fundamental and applied

characteristics of these materials. Each material is certified for chemical composition, chemical

properties, or its physical or mechanical characteristics. Each SRM is provided with a Certificate

or Certificate of Analysis that contains the essential data concerning its properties or characteristics. The SRM's currently available cover a wide range of chemical, physical, and mechanical properties, and a corresponding wide range of measurement interests in practically all aspects of fundamental and applied science. These SRM's constitute a unique and invaluable means of transferring to the user accurate data obtained at NBS, and provide essential tools that can be used

to improve accuracy in practically all areas where measurements are performed.

In addition to SRM's, the National Bureau of Standards issues a variety of Research Materials

(RM's) having various properties described in individual "Reports of Investigation." They are intended primarily to further the scientific or technical research on that particular material.

Other materials, called Special Reference Materials (GM's), are also available from NBS. These are materials produced and certified by other Government agencies, standard organizations, or other

nonprofit organizations, that are considered useful to the public and for which no alternate method

of national distribution exists.

The various categories of materials available from NBS are given in Table 1 . This table lists

these materials according to their chemical composition, physical properties, or engineering

characteristics. A more detailed alphabetic enumeration of these materials is given in Appendix I. Table 1 and Appendix I were taken from NBS Special Publication 260, NBS Standard Reference Materials

Catalog, 1984-85 Edition . This publication lists every material available from the NBS Office of Standard Reference Materials.

Further information on the reference materials available from NBS may be obtained from the Office of Standard Reference Materials, National Bureau of Standards, Gaithersburg , MD 20899. Information on other NBS services may be obtained from the Technical Information and Publications

Division, National Bureau of Standards, Gaithersburg, MD 20899.

In addition to these types of materials, NBS provides many additional services. These include: Measurement Assurance Programs, Calibration and Related Measurement Services, Proficiency Sample

Programs, a National Voluntary Laboratory Accreditation Program, Standards Information Services,

Standard Reference Data, and Technical Information and Publications.

For sale by the Superintendent of Documents, U.S. Government Printing Office, Washington,

DC 20402, under Stock No. 003-003-02558-5 (Price $5.50, add 25 percent for foreign orders.)
Table 1. Categories of Standard Reference Materials available from the National Bureau

of Standards.

CERTIFIED CHEMICAL

Steels (chip form)

Plain carbon Low alloy

High alloy

Stainless

Tool

Steels (granular form)

Steels (solid form)

Ingot iron and low alloy

Special ingot irons and low alloy

Stainless

Specialty

High-temperature alloys

Tool

Steelmaking Alloys

Cast Irons (chip form)

Cast Steels, White Cast Irons, Ductile

Irons, and Blast Furnace Irons (solid form)

Nonferrous Alloys (chip form)

Aluminum "Benchmarks"

Cobalt

Copper

Copper "Benchmarks"

Lead

Magnesium

Nickel

Nickel Superalloy, Trace Elements

Nickel oxide

Selenium

Tin

Titanium

Zinc

Zirconium

Nonferrous Alloys (solid form)

Aluminum "Benchmarks"

Copper

Copper "Benchmarks"

Lead

Nickel

Titanium

Zinc

Zirconium

ilTION STANDARDS

Gases in Metals

High-Purity Metals

Electron Probe Microanalytical Standards

Primary, Working, and Secondary

Standard Chemicals

Microchemical Standards

Clinical Laboratory Standards

Biological Standards

Environmental Standards

Analyzed gases Analyzed liquids and solids

Permeation tubes

Industrial Hygiene Standards

Forensic Standards

Hydrocarbon Blends

Metallo-Organic Compounds

Fertilizers

Ores

Minerals, Refractories, Glasses, and Carbides

Cement

Trace Element Standards

Nuclear Materials

Special nuclear materials

Plutonium assay Plutonium isotopic

Uranium assay Uranium isotopic

Neutron density standards

Fission track glass standards

Isotopic Reference Standards

2

\
Table 1. continued.

CERTIFIED PHYSICAL PROPERTY STANDARDS

Ion Activity Standards

pH standards

pD standards

Ion selective electrodes

Mechanical and Metrology Standards

Magnification

Coating thickness

Glass

Elasticity

Density

Polymer

Rheology

Heat Standards

Superconductive the rmome trie fixed

point devices

Freezing Points

Defining fixed points

Determined reference points

Melting points

Calorimetric

Combustion

Solution

Heat source Enthalpy and heat capacity

Vapor pressure Thermal expansion

Thermocouple materials

Thermal resistance

Magnetic Standards

Magnetic susceptibility

Magnetic moment Paramagnetic resonance

ENGINEERING

Standard Rubber and RubberCompounding Materials

Reference Magnetic Tapes

Centerline Drawings, OCR-B

Sizing Standards

Glass spheres for particle size

Turbidimetric and fineness (cement)

RESEARCH MATERIALS

Optical Standards

Spectropho tome trie

Thermal emittance

Refractive index

Radioactivity Standards

Alpha-particle standards

Beta-particle and gamma-ray gas standards

Alpha-particle, beta-particle,

gamma-ray, and electron-capture

solution standards

Contemporary standard for carbon-14

dating laboratories

Environmental standards Low energy photon sources Gamma-ray "point-source" standards

Radium gamma-ray solution standards

Radium solution standards for random analysis

Radioactivity standard reference

materials currently not in stock

Metallurgical

Mbssbauer

X-ray Diffraction

Gas Transmission

Permittivity

Reference Fuels

Resistivity

TYPE STANDARDS

X-ray and Photographic Standards

Surface Flammabllity Standards

Smoke Density Chamber Standards

Water Vapor Permeance

Tape Adhesion Testing Standards

Color Standards

SPECIAL REFERENCE MATERIALS

3
Biological and Botanical Standards

The first NBS biological and botanical Standard Reference Material designed specifically for the analytical laboratory use was issued in January 1971. This standard, SRM 1571, Orchard Leaves,

was developed in response to requests from numerous analytical chemists.

Since then, the number of biological and botanical SRM's has grown to 10, and during the next decade this growth is expected to continue at much the same rate.

This publication is an attempt to describe in general terms the composition, certification, and use of these biological and botanical SRM's.

Table 2 contains the essential information concerning the material, composition, the certification parameters, and use. Under "Remarks," additional data such as storage conditions and stability

is provided. All the data and information contained in this table were extracted from the Certificates of Analysis issued for the SRM's included in the table. An examination of this table gives the reader a general view of these SRM's. For more detailed information, the individual

certificates reproduced in Appendix II should be consulted as well as the references cited in each

certificate.

A summary of the quantitative distribution of the 47 chemical elements determined in the 9 Standard Reference Materials and 1 Research Material from Table 2 is presented in Table 3. The

values with an asterisk are expressed in wt. %, the others are expressed in pg/g, and the data in parantheses are non-certified values.

The certificates in Appendix II are arranged in numerical order. The SRM's listed in the table include all of the biological and botanical standards that were issued or were in preparation by the end of 1984. These SRM's are the result of the concerted efforts of a number of scientists from the NBS National Measurement Laboratory as well as those from cooperating institutions. Each certifi- cate lists the individuals who contributed to the certification of the SRM.

In addition to the SRM's and their certificates, NBS issues a series of Special Publications

(SP), called the "260 Series," that relate directly to Standard Reference Materials as stated in the Preface. The^ list of available publications in the "260 Series" is given in the beginning of this

publication.

Other NBS publications, not in the "260 Series," and a number of NBS staff authored papers have been published that deal with specific SRM's or measurement techniques. Some of these are: SP 148,

The Role of Standard Reference Materials in Measurement Systems; SP 378, Accuracy in Spectrophotometry and Luminescence Measurements (255 pp., 1973); and SP 466, Standardization in Spectrophotometry and Luminescence Measurments (150 pp., 1977), contains papers of interest to analytical

chemists. Another publication that should be of particulate interest to the users of the SRM's described in Table 2 is SP 492, "Procedures Used at the National Bureau of Standards to Determine

Selected Trace Elements in Biological and Botanical Materials."

This work consists of a collection of analytical procedures used in the Center for Analytical

Chemistry, National Measurement Laboratory of the National Bureau of Standards for the determination

of trace levels of Ag, Al , As, Be, Bi, Ca, Cd, Cr, Cu, Fe, Hg, K, Mg, Mn, Mo, Na, Ni, Pb, Pt, Sb,

Se, Te , Tl , V, and Zn in biological and botanical materials. These procedures were critically

selected or adapted, and often specially developed, by the scientific staff members of the Center

for Analytical Chemistry to provide measurements with the best obtainable accuracy. They were considered to be most appropriate for the analysis and certification of various Standard Reference

Materials issued by NBS such as SRM 1577a, Bovine Liver, SRM 1567, Wheat Flour, etc.

The description of these procedures is given with sufficient detail to permit the analyst to use them as a protocol for routine analyses in the laboratory.

They are assembled according to the analytical disciplines involved in the measuring process:

sample preparation, neutron activiation analysis, spark source mass spectrometric isotope dilution,

atomic absorption and flame emission spectrometry, molecular absorption spectrometry, fluorescence

spectrometry, and polarography

.

4
Special Publication 492 contains also a detailed description of the methods and instrumentation used to produce high-purity reagents, including the analytical techniques used to test their

purity.

The important subject of blanks, a determining factor common to all analytical measurements, is examined including a detailed description of the environmental conditions necessary to control this

essential factor, and to insure a maximum protection against contamination.

The lyophilization method for preconcentration and drying of various analytical samples is also discussed in this work.

The analytical procedures assembled in these publications are believed to provide the best measuring capabilities available at this time. They are, however, continuously being revised,

improved, or replaced by more accurate ones.

Hence, it is recommended that the analyst who desires to be informed of these advancements

maintain a contact with the scientific staff of the Center for Analytical Chemistry at NBS.

Another work, NBS Special Publication 422, "Accuracy in Trace Analysis: Sampling, Sample

Handling, Analysis," Vol. I and II, P. D. LaFleur, Editor, issued in 1976 as a proceeding of the 7th Materials Research Symposium held at NBS in 1974, contains valuable information on the subject, to the analytical chemist.

NOTE: The use of proprietary designations in Table 2 is for information only, and should not be construed as an endorsement of the product by either the Department of Commerce or the National Bureau of Standards.

For complete bibliographic reference and ordering information, see "Other NBS Publications in This

Series," pp. iv.

5
Table 2. Summary of the Biological and Botanical Standards

SRM Material Composition

RM50

Albacore Tuna

Tuna fish muscle tissue from

albacore tuna caught in the San Diego, California area.

It was frozen, ground, mixed,

lyophilized, ground again,

placed in polyethylene bags, and canned under nitrogen.

To improve homogeneity the material was reground , re- blended and recanned.

Minor elements: K 1.22%, Na

0.11%. Trace elements (ppr):

Hg 0.95; Se 3.6; Zn 13.6; As 3.3; Pb 0.46; Mn 1.3. Organic constituents (ppm):

heptadiene 0.6; toluene 0.7; limonene 0.4; 2-nonanone 0.7; 2-undecanone 0.1; 2,6-di-tbutyl-p-cresol 1.0; hexadecane, trace; heptadecane,

trace; pristane, 0.03.

1549 This material was obtained Non-Fat Milk Powder from a commercial source as

a portion of a single lot. The moisture content of the material was reduced to a low level by the spray process

.

Certified constituents:

(wt. %) Ca 1 .30; CI 1 .09; Mg 0.120; P 1.06; K 1.69; Na 0.497; S .351; (yg/g)

Cd .0005; Cr .0026; Cu .7; Fe 1.78; I 3.38; Pb 0.019; Mn .26; Hg .0003; Se .11; Zn 46.1. Non-certified

values given for 11 elements;

also for lactose and ascorbic

acid

.

The oysters were obtained by the FDA Bureau of Shellfish

Sanitation from a commercial

source, frozen in sealed

plastic bags. The material

was ground , f reeze-dried and powdered at the U.S. Army

Natick Research & Development

Command, Mass., blended and bottled at NBS and f reezedried again.

Certified minor constituents:

Ca 0.15%; Mg 0.128%; K 0.969%; Na 0.51%. Trace constituents

(yg/g): As 13.4; Cd 3.5; Cr 0.69; Cu 63.0; Fe 195; Pb 0.48; Mn 17.5; Hg 0.057;

Ni 1 .03; Rb 4 .45; Se 2.1; Ag 0.89; Sr 10.36; U 0.116;

V 2.3; Zn 852. Non-certified

information: CI (1.0%);

S (0.76%); P (0.81%); (yg/g) Br (55); Co (0.4); F (5.2);

I (2.8); Mo (£0.2); Tl (£0.005); Th (0.1). Homo- geneity was determined by neutron activation and atomic

absorption spectrometry on Na, CI, V, Mn, Mg, K, Cu, Zn, Cd. Calcium exhibits some inhomogeneity

.

6
Certification Use Remarks

RM 50 is not an SRM, hence, RM 50 is intended to be used RM 50, sealed in metal cans, none of the data presented in the measurement of inorganic should have an indefinite

are certified. For further and organic chemical species in storage life under normal

information see the Report of marine tissue at trace concen- room conditions. The open Investigation describing this trations. It should be useful sample can be kept for 6 Research Material. to scientists interested in months to 2 years in a polyevaluating analytical methods ethylene bag at 0 °C. and interlaboratory comparisons.

The analytical methods used in the certification were: atomic

absorption spectrometry; atomic

emission spectrometry; ion

chromatography, isotope dilution

mass spectrometry, neutron activation, and photon activation.

All measurements are based on a minimum 500 mg of the dried

material

.

SRM 1549 is intended for use in calibrating instrumentation and evaluating the reliability of analytical methods for the determination of constituents in milk, milk powders, and other

biological matrices.

The material should be kept

in its original bottle and stored at temperatures be- tween 10-30 °C. It should not

be exposed to intense sources

of radiation. The bottle

should be kept tightly closed

and stored in a desiccator in

the dark. This certification

is invalid after 3 years from date of shipping.

The analytical methods used in the certification were: atomic

absorption, flame and spark

emission spectrometry, inductively coupled plasma, thermal

ionization and isotope dilution,

isotope dilution mass spectrometry, spark source mass spectrometry; neutron activation,

and polarography . All measure- ments are based on a minimum

250 mg of the dried material.

SRM 1566 is intended for calibrating instrumentation and validating methodology for the chemical analysis of marine

animal tissue for minor and trace elements.

The material should be kept

in its original bottle, stored

at 10-30 °C in a dark desiccator. Under these conditions

it should be stable for 5 years from date of shipping.

7
Table 2. Biological and Botanical Standards.

SRM Material Composition

1567 Wheat Flour

Wheat flour was milled from a blend of Hard Red Spring and Hard Red Winter wheat grown in South Dakota and subjected to 2.5 megarads of Co-60 radiation

for microbiological control at Neutron Products, Inc.,

Dickerson, Md. The material

was passed through a sieve with

openings of 425 ym and blended.

Certified minor constituents: K 0.136%; Ca 0.019%. Trace constituents (yg/g): Fe 18.3; Zn 10.6; Mn 8.5; Na 8.0; Cu 2.0; Se 1.1; Cd 0.032;

Hg 0.001. Non-certified

information (yg/g): Br (9); Rb (1); Mo (0.4); Ni (0.18); As (0.006); Te (£0.002).

Homogeneity was determined by

instrument neutron activation measurements on Mn, K, Zn, Na, and Br.

1568

Rice Flour

Rice flour was prepared from

100% long grain from Arkansas

and subjected to 2.5 megarads

of Co-60 radiation for microbiological control at Neutron

Products, Inc., Dickerson, Md. The material was passed through

a sieve with openings of 425 ym

and blended.

Certified minor constituents: K 0.112%; Ca 0.014%. Trace constituents (yg/g): Mn 20.1; Zn 19.4; Fe 8.7; Na 6.0; Cu 2.2; As 0.41; Se 0.4; Cd 0.029; Co 0.02; Hg 0.0060.

Non-certified information

(yg/g): Rb (7); Mo (1.6); Br (1); Ni (0.16); Te (£0.002)

Homogeneity was determined by

instrumental neutron activation measurements on Mn, K, Zn, Na, and Br.

1569 Brewers Yeast

Yeast was obtained from the Nutrition Institute, U.S. Dept.

of Agriculture, Beltsville, Md. It was sieved (0.15 mm) and blended

.

Cr, 2.12 ± 0.05 yg/g determined on the sample

without drying and calculated

on a dry weight basis. Homogeneity was, determined by a neutron activation technique.

1572 Citrus Leaves

The plant material was collected

from the Lake Alfred area of central Florida and prepared at Michigan State University. It was air-dried, ground to pass a 425 ym sieve, dried at 85 °C and mixed. It was sterilized with

Co-60 radiation at the U.S. Army

Research & Development Command,

Natick, Mass.

Certified major and minor

constituents (wt. % on dry

weight basis): Ca 3.15; Mg 0.58; P 0.13; K 1.82;

S 0.407. Trace constituents

(yg/g): Al 92; As 3.1; Ba 21; Cd 0.03; Cr 0.8; Cu 16.5; I 1.84; Fe 90; Pb 13.3; Mn 23; Hg 0.08; Mo 0.17; Ni 0.6; Rb 4.84; Na 160; Sr 100, Zn 29. Non- certified values (yg/g):

Sb (0.04); Br (8.2); Ce (0.28); Cs (0.098); CI (414); Co (0.02) Eu (0.01); La

(0.19); Sm (0.052); Sc (0.01);

Se (0.025); Te (0.02) Tl

(£0.01); Sn (0.24); U (£0.15);

N (2.86 wt. %).

8
Certification Use Remarks

The analytical methods used in

the certification were: atomic

absorption, flame emission, and isotope dilution spark source mass spectrometry, neutron

activation, and polarography

.

All measurements are based on

a minimum 400 mg sample and are reported on a "dry-weight"

basis. Selenium and mercury

are determined on the material

without drying.

SRM 1567 is intended for cali- brating instrumentation and evaluating the reliability of analytical methods for the determination of minor and trace elements in wheat flour

and similar food products.

The material should be kept

in its original bottle, stored

at 10-30 °C in a dark desiccator. Under these conditions

it should be stable for 5 years from date of shipping.

Same as SRM 1567. Same as SRM 1567. Same as SRM 1567

Chromium w?s determined by neutron activation and by isotope dilution mass spectromtry.

The analyses were performed on the sample without drying and the results expressed on a dry

weight basis established on separate samples heated at 85 °C for 3 hrs. The samples

should not be dissolved in open vessels

.

SRM 1569 is intended for use in calibrating instrumentation and evaluating the accuracy of analytical methods for the determination of chromium in Brewers Yeast and other biological materials containing a volatile chromium component which

presents an especially difficult

problem.

The material should be kept

in its original bottle tightly

capped, and stored at 10-23 °C

in a dark desiccator.

The analytical methods used in the certification were: atomic

absorption and flame emission

spectrometry, atomic emission

spectrometry using inductively

coupled plasma, ion chromatography, isotope dilution

thermal source mass spectrometry, isotope dilution spark source mass spectrometry,

Kjeldahl method for nitrogen, neutron activation, photon

activation, polarography,

spectrophotometry.

SRM 1572 is intended for use in calibrating instrumentation and evaluating the analytical methods

used for the determination of major, minor, and trace elements

in botanical materials, agricultural food products, and similar

matrices

.

The material should be kept

tightly closed in its original

bottle, stored in a dark

desiccator at a temperature

between 10-30 ° C. A minimum

sample of 500 mg of the dried

material should be used for analyses

.

9
Table 2. Biological and Botanical Standards.

SRM Material Composition

1573 Tomato LeavesThe leaves were obtained from

a field of direct seeded tomatoes at the Horticultural

Research Center of the Michigan

State University. The airdried leaves were ground, dried

at 85 °C, mixed, packaged in polyethylene-lined drums, and sterilized with Co-60 radiation

at the U.S. Army Research & Development Command, Natick,

Mass.

Certified major and minor con- stituents (wt. %): K 4.46; Ca 3.00; P 0.34. Trace con- stituents (ug/g): Fe 690; Mn 238; Zn 62; Sr 44.9; Rb 16.5; Cu 11; Pb 6.3; Cr 4.5; As 0.27; Th 0.17;

U 0.061. Non-certified information, major and minor con- stituents (wt. %): N (5.0);

Mg (0.7); Al (0.12). Trace constituents (yg/g): B (30); Br (26); Cd (3); Ce (1.6); La (0.9); Co (0.6); Sc (0.13);

Hg (0.1); Tl (0.05); Eu (0.04)

Homogeneity was evaluated by

determining P, Fe, Mn, Zn, Rb,

Cu, Cr, As, and U.

1575

Pine Needles

Collected from the Manistee

State Park, Mich., Air-dried,

ground, and dried again at 85 °C. It was mixed, passed

through a 0.25 mm sieve, packaged in polyethylene drums, and sterilized with Co-60 radiation

at the U.S. Army Research & Development Command, Natick, Mass.

Certified minor elements:

Ca 0.41%; K 0.37%; P 0.12%. Trace constituents (pg/g): Mn 675; Al 545; Fe 200; Rb 11.7; Pb 10.8; Sr 4.8; Cu 3.0; Cr 2.6; As 0.21; Hg 0.15; Th 0.037; U 0.020.

Non-certified information:

N ( 1 .2%) ; and Br (9) ; Ni (3.5)

Ce (0.4); Cd (<0.5); Sb (0.2); La (0.2); Co (0.1); Tl (0.05); Sc (0.03); Eu (0.006) yg/g.

Homogeneity was established by

determining P, Al , Fe, Mn, Rb,

Cu, Cr, As, Hg, U, K, Ca, Sr, Pb, and Th.

1577a

Bovine Liver

The liver was obtained in the Portland, Oregon area. It was ground, mixed, and lyophilized

in polyethylene trays by

Oregon Freeze Dry Food Inc.,

Albany, Oregon. The material

was then powdered and packaged

in moisture-proof bags.

Certified elements (wt. %): CI 0.28; P 1.11; K 0.996; Na 0.243; S 0.78. Trace elements

(pg/g): As 0.047; Cd 0.44; Ca 120; Co 0.21; Cu 158; Fe

194; Pb 0.135; Mg 600; Mn 9.9; Hg 0.004; Mo 3.5; Rb 12.5; Se 0.71; Ag 0.04; Sr 0.138; U 0.00071; V 0.099; Zn 123. Non-certified information:

N (10.7 wt. %); and (ug/g);

Al (2); Sb (0.003), Br (9); Tl (0.003). Homogeneity was tested by analyzing randomly

selected samples and found to be satisfactory.

10
Certification Use Remarks

The analytical methods used in

the certification were: atomic

absorption and optical emission

spectroscopy, isotope dilution

and spark source mass spectrometry, Kjeldahl, neutron activation, nuclear track technique,

spectrophotometry, polarography

.

A minimum of 500 mg of the dried

material should be used for the analysis.

The analytical methods used in the certification were: atomic

absorption spectroscopy, isotope

dilution mass spectrometry,

isotope dilution spark source mass spectrometry, Kjeldahl

method, neutron activiation,

nuclear track technique, optical

emission spectroscopy, spectrophotometry, polarography. A minimum of 500 mg of the dried

material should be used for the analysis.

The analytical methods used in the certification were: atomic

absorption and flame emission

spectrometry, ion chromatogrphy

,

inductively coupled plasma emission spectrometry, isotope dilution thermal and spark source mass spectrometry, Kjeldahl

method for nitrogen, neutron

activation, polarography, and spectrophotometry. A minimum of 250 mg of the dried material

should be used for the analysis.

SRM 1573 is intended for calibrating instrumentation and validation of analytical methods

for the determination of major,

minor, and trace elements in botanical materials and other

agricultural products.

Same as SRM 1573.

SRM 1577a is intended for calibration of instrumentation and validation of methods in the chemical analysis of animal

tissue for major, minor, and trace elements.

The material should be kept

in the original bottle stored

at 10-30 °C in a dark desiccator. Under these conditions SRM 1573 should be stable for 5 years after

shipping date.

Same as SRM 1573.

Same as SRM 1573.

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13
Appendix i.

Name SRM Name SRM

Acetanilide 141c Aluminum, Freezing Point Standard 44f Acid Open-Hearth Steel. 0.2% Carbon 19G Aluminum, Magnetic Gram 763 Acid Potassium Phthalate 84j Susceptibility

A1SI 1045 Steel 20g Aluminum Oxide, Melting Point 742 A1SI 4340 Steel 361 Aluminum Rod Ultra Purity RM 1R

AIS1 4340 Steel 1261a Aluminum-26 Radioactivity Standard 4229

AISI 94B17 Steel (Modified) 362 Americium-241 Alpha-Particle 4904F

AIS1 94B17 Steel (Modified) 1262a Standard

Albacore Tuna RM 50 Americium-241 Gamma-ray Standard 4213

Alkali Lead Silicate Glass 712 Ammonium Dihydrogen Phosphate 194 Alpha Quartz 1878 Angiotensin I (Human) 998 Alumina (Reduction Grade) 699 Anisic Acid 142 Alumina Silicate Glass 714 Anticonvulsant Drug Level Assay i 3vs> Aluminosilicate Glass 715 Standard

Aluminum Alloy 85B Antiepilepsy Drug Level Assay

Aluminum Alloy 6011 (Modified) Of o 538 Standard

A 1 * All S f~\ 1 1 f \. M 1 C \\ Aluminum Alloy 6011 (Modified) 1258 Antimony - 1 25-Tellurium - 1 25m, 4/ /3b

A 1 All n/nr Aluminum Alloy 7075 859 Europium- 1 54, Europium-155 MixedAluminum Alloy 7075 1 Ten

1 23v fxtiUKMiuciiue r onu-oouice oiauuaiu

Aluminum Block, hddy Current 1 860 Ant imony - 1 2 5 -Tel 1 uri urn- 125m, /OD

Conductivity Europium- 1 54, Europium- 1 55 MixedAluminum Block, Eddy Current 1861 Radionuclide Solution Standard

Conductivity A O H 0 4C Snertrnpninhir Steel T411 J3 Aluminum Block, Eddy Current 1862 Standard

Conductivity Argillaceous Limestone 1C

Aluminum Block, Eddy Current 1863 Arsenic Trioxide Reductometric 83d

Conductivity Standard

Aluminum Brass Standard for 1 118 Assay-Isotopic Standard for Potassium 985

Optical Emission and X-ray Assay-Isotopic Standard for Rhenium 989

Spectroscopic Analysis Assay-Isotopic Standard for Silicon 990

Aluminum Brass Standard for C 1 1 1

8

Assay-Isotopic Standard /or Strontium 987

Optical Emission and X-ray 2% Austenite in Ferrite 488

Spectroscopic Analysis 5% Austenite in Ferrite 485a Aluminum Brass Standard for 1 1 19 15% Austenite in Ferrite 486

Optical Emission and X-ray }0% Austenite in Ferrite 487

Spectroscopic Analysis

Aluminum Brass Standard for CI 1 19 Optical Emission and X-ray

Spectroscopic Analysis

Aluminum Casting Alloy 356 855 Aluminum Casting Alloy 380 856 Aluminum Cube Ultra Purity RM 1C

Aluminum 2-Ethylhexanoate 1075a

14
Name SRM Name SRM

Austenitic Stainless Steel, Thermal 1460 Beryllium on Filter Media 2675 Conductivity and Electrical Bessemer Steel (Simulated) Hi"J Resistivity 0.1% Carbon

Austenitic Stainless Steel, Thermal 1461 Bilirubin 916

Conductivity and Electrical Bis( 1 -phenyl- 1 , 3-butanediono) 1080a

Resistivity copper (II) Austenitic Stainless Steel, Thermal 1462 Bis( 1 -phenyl- 1 , 3-butanediono) 1052b Conductivity and Electrical oxovanadium (IV)

Resistivity Black Porcelain Enamel for Directional 2021 Barium Crown Glass 713 Hemispherical Reflectance Barium Cyclohexanebutyrate 1051b Black Porcelain Enamel for Directional 2022 Barrium-133 Radioactivity Point-Source 4241

B

Hemispherical Reflectance Standard Blast Furnace Iron Standard 1143a Barium- 133 Radioactivity Standard 425 IB (Chill Cast White)

Basalt Rock 688 Blast Furnace Iron Standard 1 144a Base Oil 1083 (Chill Cast White)

Basic Electric Spectrographic Steel 404a B.O.H., 0.4C Spectrographic Steel 417a

Standard Standard

Basic Open-Hearth Steel, 0.1% Carbon 15g Boric Acid 951 Basic Open-Hearth Steel, 0.1% Carbon 335 Boron-Doped Silicon Slices for 1521 Basic Open-Hearth Steel, 0.1% Carbon 1228 Resistivity Measurements

Basic Open-Hearth Steel, 0.2% Carbon 1 lh Borosilicate Glass 93a

Basic Open-Hearth Steel, 0.4% Carbon 12H Borosilicate Glass 623

Basic Open-Hearth Steel, 0.5% Carbon 152A Borosilicate Glass 717

Basic Open-Hearth Steel, 0.8% Carbon 14f Borosilicate Glass 1825

Basic Open-Hearth Steel, 1% Carbon 1227 Borosilicate Glass, Thermal Expansion 731 (Disk) Bovine Liver 1577a

Basic Open-Hearth Steel, 1.1% Carbon 16f Bovine Serum Albumin 926

Basic Open-Hearth Steel, 1.1% Carbon 337 Bovine Serum Albumin (7% Solution) 927 0.4C Basic Oxygen Furnace Steel 178 Branched Polyethylene 1476

Bauxite (Arkansas) 69b Brewers Yeast 1569 Bauxite (Dominican) 697 Bright Copper Microhardness 1894 Bauxite (Jamaican) 698 Standard

Bauxite (Surinam) 696 Bright Nickel Microhardness Standard 1895 Benzene in Nitrogen 1805 Bromobenzoic Acid 2142 Benzene in Nitrogen 1806 Burnt Refractory 76a Benzene Permeation Device 1911 Burnt Refractory 77a Benzoic Acid 140b Burnt Refractory 78a Benzoic Acid 350a Cadmium Cyclohexanebutyrate 1053a Benzoic Acid Calorimetric Standard 39i Cadmium, Vapor Pressure 746

Benzothiazyl Disulfide Rubber 373f Calcium Carbonate 915 Compound Calcium 2-Ethylhexanoate 1074a Beryllium-Copper Standard 1122 Calcium in Low-Alloy (Silicon) Steel 1254 Beryllium-Copper Standard CI 122 Calcium Molybdate 71 Beryllium-Copper Standard CI 123 Calibrated Glass Beads 1004

Calibrated Glass Beads 1017a

Calibrated Glass Beads 1018a

Calibrated Glass Spheres 1003a Carbon Dioxide in Air 1670 Carbon Dioxide in Air 1671 Carbon Dioxide in Air 1672 Carbon Dioxide in Nitrogen 1674b Carbon Dioxide in Nitrogen 1675b Carbon Dioxide in Nitrogen 2619a (Combustion Efficiency Gas Standard)

Carbon Dioxide in Nitrogen 2620a (Combustion Efficiency Gas Standard)

15
Name SRM

Carbon Dioxide in Nitrogen 2621a

(Combustion Efficiency Gas Standard)

Carbon Dioxide in Nitrogen 2622a

(Combustion Efficiency Gas Standard)

Carbon Dioxide in Nitrogen 2623a

(Combustion Efficiency Gas Standard)

Carbon Dioxide in Nitrogen 2624a

(Combustion Efficiency Gas Standard)

Carbon Dioxide in Nitrogen 2625a

(Combustion Efficiency Gas Standard)

Carbon Dioxide in Nitrogen 2626a

(Combustion Efficiency Gas Standard)

Carbon Dioxide in Nitrogen (Mobile 2632 Source Emission Gas Standard)

Carbon Dioxide in Nitrogen (Mobile 2633 Source Emission Gas Standard)

Carbon Monoxide in Air (Ambient 2612a

Air Quality Gas Standard)

Carbon Monoxide in Air (Ambient 2613a

Air Quality Gas Standard)

Carbon Monoxide in Air (Ambient 2614a

Air Quality Gas Standard)

Carbon Monoxide in Nitrogen 1677c Carbon Monoxide in Nitrogen 1678c Carbon Monoxide in Nitrogen 1679c Carbon Monoxide in Nitrogen 1680b Carbon Monoxide in Nitrogen 1681b Carbon Monoxide in Nitrogen (Mobile 2635 Source Emission Gas Standard)

Carbon Monoxide in Nitrogen (Mobile 2636 Source Emission Gas Standard)

Carbon Monoxide in Nitrogen (Mobile 2637 Source Emission Gas Standard)

Carbon Monoxide in Nitrogen (Mobile 2638 Source Emission Gas Standard)

Carbon Monoxide in Nitrogen (Mobile 2639 Source Emission Gas Standard)

Carbon Monoxide in Nitrogen (Mobile 2640 Source Emission Gas Standard)

Carbon Monoxide in Nitrogen (Mobile 2641 Source Emission Gas Standard)

Carbon Monoxide in Nitrogen (Mobile 2642 Source Emission Gas Standard)

Carbon- 14 Radioactivity Standard 4245

Carbon-14 Radioactivity Standard 4246

Carbon Steel 1224 Carbon Steel, 0.6% 13g

Cast iron 4k

Cast Iron 5L

Cast Iron 6g

Cast Iron 7G

Cast Iron Car Wheel 122h

Cast Steel 3 CI 173 Cast Steel Standard 1138a

Cast Steel Standard 1 ]39a

Name SRM

Catalyst Package for Lubricant 1817 Oxidation

Centerline Drawings for Optical 1901 Character Recognition, B

Characters Centroid Color Chart 2106

Centroid Color Kit 2107

Cesium-137, Barium-137m Point-Source 4200B

Radioactivity Standard

Cesium-137, Barium-137m Point-Source 4207

Radioactivity Standard

Cesium-137 Burn-Up Standard 4233B

Cesium- 134 Radioactivity Standard 4250B

Channel Black Rubber Compound 375g

Chlorine-36 Beta-ray Standard 4943

Chlorine-36 Radioactivity Standard 4422L

Chlorobenzoic Acid 2144 Chrome Refractory 103a Chromium-Molybdenum-Aluminum 106B

Steel Chromium-Molybdenum Steel 36b

Chromium-Molybdenum Steel 133B

Chromium-Nickel-Molybdenum Steel 139b Chromium-Nickel-Molybdenum Steel 1222 17Chromium-9 Nickel-0.2 Selenium Steel 339 Chromium-Nickel Spectrographic Steel 408a

Standard

15Chromium-7 Nickel Steel 344 16Chromium-4 Nickel Steel 345 Chromium-51 Radioactivity Standard 4400L-F

Chromium Steel 163 Chromium-Tungsten Steel 155 Chromium-Vanadium Spectrographic 407a

Steel Standard

Cholesterol 911a

Chrysotile Asbestos Fibers 1876

Citrus Leaves 1 572

Clinical Laboratory Thermometer 934

Cobalt Cyclohexanebutyrate 1055b Cobalt-Molybdenum-Tungsten Steel 153

A

Cobalt-57 Radioactivity Standard 4408L-C

Cobalt-60 Radioactivity Standard 49I5D

Commerical Bronze Standard for 1115

Optical Emission and X-ray

Spectroscopic Analysis

Commercial Bronze Standard for CI 1 15 Optical Emission and X-ray

Spectroscopic Analysis

Commercial Bronze Standard for 1116

Optical Emission and X-ray

Spectroscopic Analysis

16
Name SRM

Commercial Bronze Standard for CI 116 Optical Emission and X-ray

Spectroscopic Analysis

Commercial Bronze Standard for 1117

Optical Emission and X-ray

Spectroscopic Analysis

Commercial Bronze Standard for CI 117

Optical Emission and X-ray

Spectroscopic Analysis

Common Lead Isotopic Standard 981 Copper Concentrate 332 Copper Heat Capacity Test Specimen RM5

Copper-Nickel-Chromium Cast Iron 115A

Copper Ore, Mill Heads 330 Copper Ore, Mill Tails 331 Copper-Thermal Expansion 736a Copper, Secondary Freezing Point 45d

Standard

Cortisol (Hydrocortisone) 921

Creatinine 914

Cr-Mo Low Alloy Steel 1270 Cr-Mo Steel (ASTM A-2 13) 291 Cr-Mo (SAE 4140) Spectrographs 414

Steel Standard

Cr-Mo (SAE 4150) Spectrographic 427

Steel Standard

Cr-Mo (SAE X4130) Spectrographic 418a

Steel Standard

Cr-Ni-Mo Steel (AISI 8620) 293 18Cr-10Ni Steel (AISI 304L) 101f Cr-V Steel (Modified) 363 Cr-V Steel (Modified) 1263a Cr-V Steel (SAE 6150) 30f

Crystalline Potassium Dichromate 935

Crystalline Potassium Iodide. 2032 Heterochromatic Stray Radiant

Energy Standard

Crystalline (Ruby) Electron 2601 Paramagnetic Resonance

Absorption Intensity Standard

Cupro-Nickel (CDA 706) 1275 Cupro-Nickel (CDA 715) 1276 Cupro-Nickel, 10% (CDA 706) Doped 875

Name SRM

Cupro-Nickel, 10% (CDA 706) High 874

Purity

Cystine 143c Dextrose 41b

D-Glucose 917

Dibutyltin Bis(2-ethylhexanoate) 1057b Didymium Glass Filter for Checking 2009

the Wavelength Scale of Spectrophotometers

Didymium Glass Fitler for Checking

the Wavelength Scale of Spectrophotometers

Disodium Hydrogen Phosphate 18611c Disodium Hydrogen Phosphate 218611 D-Mannitol 920 Dolomitic Limestone 88a DnnpH Platinum Do 11—1 r^r\n(^H Platinum Do 1 Li

Leucine v_asi iron 1/1

1

Electrical Residual Resistivity Ratio "7 AO StanHarH

nice 1 1 uiy uc iron niecu l-.' lo/"*tf-/-»l\jti/-» IrAn uiy uc iron i zoja

niec u uiy uc lion, i nermai

Conductivity and Electrical

Resistivity

Electrolytic Iron, Thermal 1 4o4 Conductivity and Electrical

Resistivity Electronic and Magnetic Alloy 1159 Standard

Electronic and Magnetic Alloy 1160 Standard

Enriched Boric Acid 952 Equal-Atom Lead Isotopic Standard 982

Estuarine Sediment 1646 Europium- 152 Point-Source Standard 4218E

Europium- 152 Radioactivity Standard 4370B

Extra Dense Lead Glass 709 Fe-Cr-Ni Alloy Microprobe Standard 479a

Fe-3Si Alloy Microprobe Standard 483

Feldspar 70a Feldspar 99a Ferrochromium (Low Carbon) 196 Ferrochromium Silicon 689 Ferroniobium 340 Ferrophosphorus 90

Ferrosilicon 58a

Ferrosilicon 59a

Ferrosilicon (75% Si) 195

First Surface Aluminum Mirror for 2003a

Specular Reflectance

First Surface Mirror, Gold on Glass 2008a

17
Name SRM Name SRM

Fission Track Glass Standard 961 Gold- 198 Radioactivity Standard 4405 L-B

Fission Track Glass Standard 962a Gold-Silver Wires for Microprobe 481

Fission Track Glass Standard 963a Analysis

Fission Track Glass Standard 964 Gold, Vapor Pressure 745

Flint Clay 97a Gray Cast Iron 334 Fluorobenzoic Acid 2143 Halocarbons (in methanol) for Water 1639 Fluorspar 79a Analysis

Free-Cutting Brass 1103 High-Alloy Steel (A-743) C1288

Free-Cutting Brass CI 104 High-Alloy Steel (AISI 310 Mod.) C1287

Freeze-Dried Urine 2670 High-Alloy Steel, (AISI 414 Mod.) C1289

Freeze-Dried Urine Certified 2671a High-Alloy White Cast 892

for Fluoride High-Alloy White Cast Iron 890

Freeze-Dried Urine Certified 2672a High-Alloy White Cast Iron 891

for Mercury High-Carbon Ferrochromium 64c

Fused-Silica Thermal Expansion 739 High-Carbon Ferromanganese 68c Gadolinium-148 Alpha-Particle 4907 High-Carbon Steel (Modified) 364 Standard High-Carbon Steel (Modified) 1264a Gallium Melting-Point Standard 1968 High-Grade Fluorspar 180 Gallium-67 Radioactivity Standard 4416L-D High-Nickel Steel 126c Gas Furnace Black Rubber Compound 382a High-Nickel Steel 1158 Gasometric Set (1095-1099) 1089 High-Purity Gold 685 Gasometric Standard for Unalloyed 357 High-Purity Platinum 680L1A

Zirconium High-Purity Platinum 680L2A

Gasometric Standard for Unalloyed 358 High-Purity Platinum Thermoelement 1967 Zirconium High-Purity Zinc 682 Generator Columns for Polynuclear 1644 High-Silicon Steel 179 Aromatic Hydrocarbons High-Silicon Steel 1134 Gilding Metal 1 1 12 High-Silicon Steel 1135 Gilding Metal CU12 High-Silicon Steel (Calcium Bearing) 125b Gilding Metal 1113 High-Sulfur Steel 105 Gilding Metal CI 113 High-Sulfur Steel 129c Gilding Metal 1 1 14 High-Sulfur Steel 1136 Gilding Metal CI 1 14 High Temperature Alloy A286 348

Glasses for Microchemical Analysis 1871 High Temperature Alloy M308 1197 Glasses for Microchemical Analysis 1872 High TeYnperature Alloy L605 and SI 199 Glasses for Microchemical Analysis 1873 S816

Glasses for Microchemical Analysis 1874 High-Temperature Alloy 1206-2 Glasses for Microchemical Analysis 1875 High-Temperature Alloy 1207-1 Glass Fibers for Microanalysis RM 31 High-Temperature Alloy 1207-2 Glass Filter for Transmittance 2030 High-Temperature Alloy 1208-1 Measurement High-Temperature Alloy 1208-2 Glass Filters for Spectrophotometry 930D Homogeneous River Sediment for RM 45B

Glass Fluorescence Source 477 Radioactivity Measurements

Glass Sand 81a Human Liver, Environmental 4352

Glass Sand 165a Radioactivity Glass Spheres 1019a Human Lung, Environmental 4351 Gold Coating on Glass Sealing Alloy 1 398a Radioactivity

Gold Coating on Nickel 1379 Human Serum 909

Gold Coating on Nickel 1380 Gold Coating on Nickel 1 399b

Gold-Copper Wires for Microprobe 482

Analysis

Gold-195 Radioactivity Standard 4421L

18
Name

Hydrogen in Unalloyed Titanium

Hydrogen in Unalloyed Titanium

Hydrogen in Unalloyed Titanium

Hydrogen in Unalloyed Titanium

Hydrogen-3 Radioactivity Standard

Hydrogen-3 Radioactivity Standard

Hydrogen-3 Toluene Radioactivity

Standard

4-Hydroxy-3 methoxy-DL-mandelic

Acid (VMA)

ICTA High Temperature Set

Differential Thermal Analysis

ICTA Low Temperature Set Differential Thermal Analysis

ICTA Mod Temperature Set Differential Thermal Analysis

ICTA Mid Temperature Set Differential Thermal Analysis

ICTA Polystyrene Differential Thermal Analysis

ICTA Thermogravimetry Set Incoloy, 901 and Hastelloy X

Inconels, Alloy 600 (Chips)

Inconels, Alloy 600 (Solid)

Inconels, Alloy 625 (Chips)

Inconels, Alloy 625 (Solid)

Incoloy, Alloy 800 (Chips)

Incoloy, Alloy 800 (Solid)

Incoloy, Alloy 825 (Chips)

Incoloy, Alloy 825 (Solid)

Indium-Ill Radioactivity Standard

Ingot Iron Spectrographic Steel Standard

Intermediate Purity Selenium

Intermediate-Purity Zinc

Iodine- 123 Radioactivity Standard

Iodine- 125 Radiactivity Standard

Iodine- 129 Radioactivity Standard

Iodine-131 Radioactivity Standard

Iron Foil Mossbauer Standard

Iron-55 Low-Energy Photon Standard

Iron Metal (Clinical Standard)

Iron Ore (Labrador)

Iron Ore (Nimba)

SRM Name SRM

352b Iron Ore (Sibley) 27f 1086 Iron Ore Concentrate (Canada) 690

1087 Iron-59 Radioactivity Standard 4411L-B

1088 Isobutylene-Isoprene (Butyl) Rubber 1495 4361 Isobutylene-Isoprene (Butyl) Rubber 388L

4926C Isotopic Standard for Bromine 977 4947 Isotopic Standard for Chlorine 975

Isotopic Standard for Chromium 979

925 Isotopic Standard for Copper 976

Isotopic Standard for Magnesium 980

GM 760 Isotopic Standard for Silver 978 Krypton-85 Gaseous Radioactivity 4308C

GM 757 Standard

Krypton-85 Radioactivity Standard 4235

GM 759 Krypton-85 Radioactivity Standard 4935C

Lead-Barium Glass 89 GM 758 Lead-Base Bearing Metal 53e Lead-Base Bearing Metal 1132 GM 754 Lead Cyclohexanebutyrate 1059c Lead in Reference Fuel 1636a GM 761 Lead in Reference Fuel 1637a

SI 198 Lead in Reference Fuel 1638a 864 Lead Nitrate 928

1244 Lead on Filter Media 2674

865 Lead-203 Radioactivity Standard 4420L

1245 Lead, Secondary Freezing Point 49e

866 Standard

1246 Lead-Silica Glass 1827 867 Lead-Silica Glass (Viscosity) 711 1247 Lead-Silica Glass for dc Volume 624 4417L-C Resistivity

420a Lead-Silica Glass for Dielectric 774 Constant 726 Lead 206 Spike Assay and Isotopic 991 728 Solution Standard

4414L-C Leaded-Tin Bronze Alloy 1035 4407L-H Light-Sensitive Paper 700d

4949B Light-Sensitive Paper 701d

4401 L-I Light-Sensitive Plastic Chip 703

1541 Linear Polyethylene 1475 4260C Linear Polyethylene 1482 937 Linear Polyethylene 1483 692 Linear Polyethylene 1484 693 Linerboard, Standard for Tape 1810 Adhesion Testing

Liquid Absorbance Standard for 931c

Ultraviolet and Visible Spectrophotometry

Lithium Carbonate 924

Lithium Ore 181 Lithium Ore 182 Lithium Ore 183 Low-Alloy Steel, (AISI 4130) 1225 Low Alloy Steel 1226 Low Alloy Steel (A242 Mod.) C1285

Low-Alloy Steel, AISI 4130 72g

Low Alloy Steel (AISI 1526, Modified) 1269 Low-Alloy Steel (Hy 80) 1286

19
Name SRM Name SRM

Low-Alloy -Meet J>et (OOl-Oo?) CAAQ 0OO0 Naval Brass Standards for Optical 1108 Low-Carbon Silicon Steel 111/- 1 J I L Emission and Spectroscopic

Low-Carbon Silicon Steel i mia Analysis

Low-Carbon Stainless Steel (AISI 1 fyf\r I DDL Naval Brass Standards for Optical CI 108

1 1 A I \ i l oL

)

Emission and Spectroscopic

Magnesium-base Alloy 1 7 1

1 / 1 Analysis

Magnesium Cyclohexanebutyrate 11 0A1 UD I rL Neutral Glass 716 Magnesium Gluconate Dihydrate Q7Q y Ly Neutron Density Monitor Wire 953 Magnetic Coating on Magnetic 1 3ASa 1 JD ja Nickel-Chromium Cast Iron 82b

Substrate (Nickel on Steel) Nickel-Chromium-Molybdenum Cast Iron 107C

Magnetic Coating on Magnetic 1 1AA.Q i ->ooa Nickel-Chromium Steel 32E

Substrate (Nickel on Steel) Nickel-Copper Alloy 882 Magnetic Coating on Non-Magnetic 1 1A7^ i jo /a Nickel Cyclohexanebutyrate 1065b

Substrate (Nickel and Chromium Nickel Oxide, No. 1 671 on brass Nickel Oxide, No. 2 672 Magnetic Tape, High Density a 7 OZjU Nickel Oxide, No. 3 673 Manganese Fluoride, Magnetic Gram 7AA / OO Nickel-63 Radioactivity Standard 4226

Susceptibility Nickel Silver (CDA 762) 879 Manganese Ore Zju 7 Nickel Siver (CDA 770) 880 Manganese-54 Point-Source 4W 1 fc. Nickel Spectrographic Steel Standard 409b

Radioactivity Standard Nickel Sphere, Magnetic Moment 772 Manganese-54 Radioactivity Standard 4ZJ A~> S7/ Nickel Steel 33d

Manganese Steel i Ann Ni-Cr-Mo-V Steel 1173 Manganous Cyclohexanebutyrate i nA7i-> i uozrj Nicotinic Acid 148 Maraging Steel 1 1 SA 1 1 DO Niobium-94 Gamma-ray Standard 420 IB

Metal on Quartz Filters for ZUj 1 Nitric Oxide in Nitrogen 1683b

Spectrophotometry Nitric Oxide in Nitrogen 1684b

Metals on Filter Media ZD7 A7AK / OD Nitric Oxide in Nitrogen 1685b Methane in Air I ODoa Nitric Oxide in Nitrogen 1686b Methane in Air 1

i ASQa o jva Nitric Oxide in Nitrogen 1687b Methane in Air 1

i AAMj ooua Nitric Oxide in Nitrogen (Mobile 2627

Medium Manganese Spectrographs 4UJd Source Emission Gas Standard)

Steel Standard Nitric Oxide in Nitrogen (Mobile 2628 Mercapt oben zot hiazole JOJd Source Emission Gas Standard)

Mercury, Freezing Point 1A1 /4j Nitric Oxide in Nitrogen (Mobile 2629 Mercury-203 Radioactivity Standard AA44 1 fi I

1 8L Source Emission Gas Standard)

Mercury in Water, jug/mL 1 f,A\h, 1 041D Nitric Oxide in Nitrogen (Mobile 2630

Mercury in Water, ng/mL 1

1 A47K 04ZD Source Emission Gas Standard)

Microcopy Resolution Test Chart 1 n 1 Ho 1 u i ua Nitric Oxide in Nitrogen (Mobile 2631 Microprobe Standard — Cartridge Brass 478 4 / o Source Emission Gas Standard)

Mineral Glasses for Microanalysis 4 / U Nitrogen Dioxide in Air (Stationary 2653 Molybdenum Concentrate j jj Source Emission Gas Standard)

\A i ~\ 1 v h/H ti 1 1 m M^'jt I a r\ 'i c 1 t \i iviv. m v uuc ii u 1 1 1

, ileal v^ai'ueity 781 Nitrogen Dioxide in Air (Stationary 2654

Molybdenum-99 Radioactivity AA44 171 1 ZL-riH Source Emission Gas Standard)

Standard Nitrogen Dioxide in Air (Stationary 2655

iviuiyuueuum- 1 uiigsieii-v^iiiuiniuiii- 1 TiAA Source Emission Gas Standard)

v driauiuiii oieei Nitrogen Dioxide in Air (Stationary 2656

Naval Rracc ^tanHarH? for f )ntipsil 1 106 Source Emission Gas Standard)

Emission and Spectroscopic Nitrogen Dioxide Permeation Device 1629a

Analysis 4-Nitrophenol 938 Naval Brass Standards for Optical CI 106 Emission and Spectroscopic

Analysis

Naval Brass Standards for Optical 1107 Emission and Spectroscopic

Analysis

Naval Brass Standards for Optical CI 107 Emission and Spectroscopic

Analysis

20
Name

Nodular Cast Iron Nominal One Micrometer Polystyrene

Spheres Non-Fat Powdered Milk

Nonmagnetic Coating on Magnetic

Substrate (Copper and Chromium

on Steel) Nonmagnetic Coating on Magnetic

Substrate (Copper and Chromium

on Steel) Nonmagnetic Coating on Magnetic

Substrate (Copper and Chromium

on Steel) Nonmagnetic Coating on Magnetic

Substrate (Copper and Chromium

on Steel) Nonmagnetic Coating on Magnetic

Substrate (Copper and Chromium

on Steel) Nonmagnetic Coating on Magnetic

Substrate (Copper and Chromium

on Steel) NPL GM Alpha Alumina

NPL GM Alpha Alumina

NPL GM Alpha Alumina

NPL GM Alpha Alumina

NPL GM Graphitized Carbon Black NPL GM Graphitized Carbon Black NPL GM Melting Point Set NPL GM Non-porous Silica NPL GM Non-porous Silica N-tertiary-Butyl-2-benzothiazolesulfenarnide Rubber Compound

Obsidian Rock

Octaphenylcyclotetrasiloxane

Oil Furnace Black Rubber Compound

Opal Glass Powder

Optical Emission and X-ray

Spectroscopic Analysis

Optical Microscope Linewidth Measurement Standard

Optical Microscope Linewidth Measurement Standard

Optical Microscope Linewidth Measurement Standard

IS! " mo

342a Organics in Shale Oil 1580 1690 Oxalic Acid 4990C

Oxygen in Ferrous Materials 1090

1549 Ingot Iron 1359 Oxygen in Ferrous Materials 1091

(Stainless Steel AISI 431)

Oxygen in Ferrous Materials Vacuum 1092 1360 Melted Steel Oxygen in Maraging Steel 1094 Oxygen in Nitrogen (Gas Standard) 2657

1361b Oxygen in Nitrogen (Gas Standard) 2658 Oxygen in Nitrogen (Gas Standard) 2659 Oxygen in Titanium-Base Materials 355

1362a Oxygen in Valve Steel 1093 Oyster Tissue 1566

Palladium, Magnetic Gram 765 1363a Susceptibility Penetrant Test Block 1850 Peruvian Soil, Environmental 4355

1364a Radioactivity

Petroleum Crude Oil 1582 Phosphate Rock (Florida) 120b 8005 Phosphor Bronze (CDA 521) 871 8006 Phosphor Bronze (CDA 544) 872 8007 Phosphorized Copper, Cu VIII C125!

8008 Phosphorized Copper, Cu IX C1252

8001 Phosphorized Copper, Cu X CI 253 8002 Phosphorus-32 Radioactivity Standard 4406L-G

8000 Photographic Step Tablet 1008 8003 Pine Needles 1575 8004 Plastic Clay 98a 384d Platinum, Magnetic Gram 764

Susceptibility 278 Plutonium-238 Alpha Particle Standard 4906 B

1066a Plutonium-240 Alpha-Particle Emission- 4338

378b Rate Solution Standard

91 Plutonium-239 Alpha-Particle Solution 4331

1 102 Standard

Plutonium-242 Alpha-Particle Solution 4334B

474 Standard

Plutonium Isotopic Standard 946

475 Plutonium Isotopic Standard 947 Plutonium Isotopic Standard 948 476 Plutonium Metal 949f Plutonium Metal (Standard Matrix 945

Materia!)

Plutonium-244 Spike Assay and 996

Isotopic Standard

Polychlorinated Biphenyls in Oil 1581 Polycrystalline Alumina Elasticity 718 Standard

Polyester Plastic Film Tor Oxygen 1470 Gas Transmission

Polyisobutylene Solution in Cetane 1490 Polystyrene 1478 Polystyrene 1479 Polystyrene (Broad Molecular Weight) 706

Polystyrene (Narrow Molecular 705 Weight)

Polystyrene Spheres 1691 Portland Cement (Black) 1880

21
Name SKM Name sKIVl

Portland Cement (Blue) 635 Quartz on Filter Media 2679a

Portland Cement (Clear) 639 Quinine Sulfate Dihydrate 936

Portland Cement (Gold) 634 Radiogenic Lead Isotopic Standard 983

Portland Cement (Green) 638 Radium-226 Gamma-ray Standard 4956

Portland Cement (Pink) 637 Radium-226 Gamma-ray Standard 4957

Portland Cement (Red) 633 Radium-226 Gamma-ray Standard 4958

Portland Cement (White) 1881 Radium-226 Gamma-ray Standard 4959

Portland Cement (Yellow) 636 Radium-226 Gamma-ray Standard 4960

Portland Cement Fineness Standard 114n Radium-226 Gamma-ray Standard 4961 Potassium Chloride 2202 Radium-226 Gamma-ray Standard 4962

Potassium Chloride (Clinical Standard) 918 Radium-226 Gamma-ray Standard 4963

Potassium Chloride (Primary 999 Radium-226 Gamma-ray Standard 4964B

Chemical) Radium Standard (Blank Solution) 4952B

Potassium Chloride for Solution 1655 Radon-226 for Radon Analysis 4953C

Calorimetry Red Brass 1109 Potassium Dichromate 136d Red Brass CI 109 Potassium Dihydrogen Phosphate 200 Red Brass 1110 Potassium Dihydrogen Phosphate 1861c Red Brass ClllO

Potassium Dihydrogen Phosphate 21861 Red Brass 1111 Potassium Erucate 1076 Red Brass cnn

Potassium Feldspar 607 Reduced Iron Oxide 691 Potassium Fluoride 2203 Reference Fuel Isooctane 1816a Potassium Hydrogen Phthalate 185e Reference Fuel n-Heptane 1815a Potassium Hydrogen Tartrate 188 Reflection Step Tablet 2061 Potassium Iodide with Attenuator 2033 Refractive Index Glass 1820 Potassium Nitrate 193 Refractive Index Silicone Liquids 1823 Potassium Tetroxalate 189 Refractive Index. Soda-Lime Glass 1822 Powdered Lead Based Paint 1579 Relative Stress-Optical Coefficient 708

Priority Pollutant Polynuclear 1647 Glass Aromatic Hydrocarbons (in Resulfurized-Rephosphorized Steel C1221

Acetonitrile) Rice Flour 1568 Propane in Air 1665b River Sediment 1645 Propane in Air 1666b River Sediment, Environmental 4350B

Propane in Air 1667b Radioactivity

Propane in Air 1668b Rocky Flats Soil Number 1, 4353

Propane in Air 1669b Environmental Radioactivity

Propane in Nitrogen (Mobile Source 2643 Rubidium Melting Point 1969 Emission Gas Standard) Rutile Ore 670 Propane in Nitrogen (Mobile Source 2644 Scanning Electron Microscope 484c

Emission Gas Standard) Magnification Standard

Propane in Nitrogen (Mobile Source 2645 Scanning Electron Microscope 2069

Emission Gas Standard) Performance Standard

Propane in Nitrogen (Mobile Source 2646 Secondary Standard Flexible Disk 3210

Emission Gas Standard) Cartridge (Computer Amplitude

Propane in Nitrogen (Mobile Source 2647 Reference)

Emission Gas Standard) Secondary Standard Magnetic Tape 3200 Propane in Nitrogen (Mobile Source 2648 Secondary Standard Magnetic Tape 1600 Emission Gas Standard) Cassette Propane in Nitrogen (Mobile Source 2649 Secondary Standard Magnetic Tape 3216 Emission Gas Standard) Cartridge (Computer Amplitude

Propane in Nitrogen (Mobile Source 2650 Reference)

Emission Gas Standard) Second Surface Aluminum Mirror for 2023 Propane in Nitrogen and Oxygen 2651 Specular Reflectance (Mobile Source Emission Gas

Standard)

Propane in Nitrogen and Oxygen 2652 (Mobile Source Emission Gas

Standard)

Quartz Cuvette for Spectrophotometry 932 Quartz for Hydrofluoric Acid 1654

Solution Calorimetry

22
Name SRM

Second Surface Aluminum Mirror for 2024

Specular Reflectance Second Surface Aluminum Mirror with 2025 Wedge for Specular Reflectance Selenium-Bearing Steel 1170b Selenium-75 Radioactivity Standard 4409L-D

Sheet Brass 37E

Silica Brick 198

Silica Brick 199 Silicon-Aluminum Alloy 87a

Silicon Bronze 158A

Silicon Density Standard 1840

Silicon Density Standard 1841

Silicon Metal 57a

Silicon Powder, Spacing Standard 640a

for X-ray Diffraction

Silicon Power Device Level 1522

Resistivity Standard

Silicon Resistivity Standard for Eddy 1523 Current Testers

Silver 2-Ethylhexanoate 1077a

Silver-Gold Thermocouple Wire 733

Silver, Vapor Pressure 748

Sintered and Arc-Cast Tungsten, 1465 Thermal Conductivity and

Electrical Resistivity Sintered and Arc-Cast Tungsten, 1466 Thermal Conductivity and

Electrical Resistivity Sintered and Arc-Cast Tungsten, 1467 Thermal Conductivity and

Electrical Resistivity Sintered and Arc-Cast Tungsten, 1468 Thermal Conductivity and

Electrical Resistivity

Smoke Density Chamber Standard 1007a (Flaming Exposure Condition)

Smoke Density Chamber Standard 1006b (Non-flaming Exposure Condition)

Soda-Lime Container Glass 621 Soda-Lime Flat Glass 620 Soda-Lime Float Glass 1830 Soda-Lime Glass 1826 Soda-Lime Glass Powder 92

Name SRM

VAn>i I i rv* xnoor lilocc oOUa-LlIllC OllCCl Ul£lJ»b 1831

n I tm£* W 1 1 1 o (IIqcc OUUa-Lirilc OlllCa VJiahb 622

Cz-i/io T i m ^ 1 1

1

OUUa-LlIIlC OlMCac q (tVJlass 1 ii cc 710

^nHa.T imp ^ilir*a Ola^Q for T inuiflii^ 773 Tpmnpratnrp

QnH 1

1

i m Rir*s rhnn^tp 191a SoHinm Riparhnnatp 2191 QnH JUUl1 1U1 m1 1 1 C^s\ v -.i rhnn3 1

i " 'l l il tpu 192a SnHiiim OUU1UI11 Carhnnatp V— a 1 UUllitlL 2192

2201 Sodium Chloride (Clinical Standard) Q 1 Q Sodium Cyclohexanebutyrate ii n^Qh \juyo

Sodium Oxalate Reductometric huh

oldllUal U Sodium Pyruvate Q1f) 7 1 U

OOU1UII1 1 til dUUI die L/Ct_aIiyUI alt 187b

(^DUl Rrtrtia Ay ^

J JnoIHpt 127b Solder 1131

Snpnfll Nuolpar Containpr DOT fil\A 9940

15 eal

Snpr*ifil iJJ.'L*. lal NJiiclpar 1 i Li t_ H tl 1 Contatnpr V^VJI 1 I tl 1 1 1 L 1 SS cral , t_ il 1 . 9941

Snpfifll Nurlpar Contftinpr Tvnp A 9942

10i u gai. eal Snpcial Nnclpar Containpr Tvnp A 9943

55JJ gal. eal Special Nuclear Material Package 9910

u|iL\. ii v'l^i a|unL nigwi nun aiiu 461

I ow-Allnv Stppl Standard fRnd^

Snprtrnpranhif* Tnc*nt Iron anH 462 Low-Alloy Steel Standard (Rod)

Snpctrnpranhir uULt u wti auuiv1 lugui Tnpot Irnn i i KJ 1 1 anH ailvl 463 Low-Alloy Steel Standard (Rod)

Snpotropranhif* UuLv 11 U auillvv lllgUL Intent Trnn 11 Ull and anu 464

Low-Alloy Steel Standard (Rod)

Snprtrnpranhif* kJUV.^11 uh' uUlllv Tnpot llltvH Iron 11 Ull and ClllvJ 465 Low-Alloy Steel Standard (Rod)

Snpptrnprflnhip Intent Iron anH 466 Low-Alloy Steel Standard (Rod)

JUCL ^r^ft11rcsom Ugl dLJHlV* v\V\ \c Tncrnt lllt^Ul Iron 11U1I dllLl stnH 467 Low-Alloy Steel Standard (Rod)

Snpptrnorjinnip o i >c ^_ 1 1 j i ci 1 1 1 ^ Tnuot iiigwi Trr^n nun diiu nnH 468 Low-Alloy Steel Standard (Rod)

^r*pr*t jpcL iirAffra ugi n ir* Inont iiigui Iron nun and anu 1 166 LjUW rtllUJf JICC1 JlallUdl u 442

StanHarH JlUllUUl Vl opCC 1 1 (J£, 1 apil lt_ olallllCdS OICC1 443

Jlallual ^ nft ^ rrlLI Spectrographic Stainless Steel 444

Standard

Spectrographic Stainless Steel D849

Standard (Disc)

Spectrographic Stainless Steel D850

Standard (Disc)

Spectrographic Stainless Steel 445 Standard (Group II)

23
Name

Spectrographic Stainless Steel Standard (Group II) Spectrographic Stainless Steel Standard (Group II) Spectrographic Stainless Steel Standard (Group II) Spectrographic Stainless Steel Standard (Group II) Spectrographic Stainless Steel Standard (Group II) Spectrographic Stainless Steel Standard (Rod)

Spectrographic Stainless Steel Standard (Rod)

Spectrographic Steel Standard (Disc)

Spectrographic Steel Standard (Disc)

Spectrographic Steel Standard (Rod)

Spectrographic Steel Standard (Rod)

Spectrographic Steel Standard (Rod)

Spectrographic Steel Standard (Rod)

Spectrographic Steel Standard (Rod)

Spectrographic Steel Standard (Rod)

Spectrographic Steel Standard (Rod)

Spectrographic Steel Standard (Rod)

Spectrographic Steel Standard (Rod)

Spectrographic Steel Standard (Rod)

Spectrographic Tool Steel Standard

Spectrographic Tool Steel Standard

Spectrographic Tool Steel Standard

Spectrographic Tool Steel Standard

Spectrographic Tool Steel Standard

Spectrographic Tool Steel Standard

Spectrographic Tool Steel Standard

Spectrographic Tool Steel Standard

Spectrographic Tool Steel Standard

(Disc)

Spectrographic Tool Steel Standard

(Disc)

Spectrographic Tool Steel Standard

(Disc)

Spectrographic Zinc-Base Die-Casting

Alloy A

Spectrographic Zinc-Base Die-Casting

Alloy B

Spectrographic Zinc-Base Die-Casting

Alloy C

Spectrographic Zinc-Base Die-Casting

Alloy D

Spectrographic Zinc-Base Die-Casting

Alloy E

Spectrographic Zinc-Base Die-Casting

Alloy F

Spectrographic Zinc Spelter Standard

Spectroscopic Titanium-Base Standard

Spectroscopic Titanium-Base Standard

Spectroscopic Titanium Base Standard

SRM Name

446 Spectroscopic Titanium-Base Standard

447

Spectroscopic Titanium-Base Standard

Spectroscopic Titanium-Base Standard

448

Spheroidized Iron Carbide in Ferrite Spreading Resistance Calibration

449

(100) n-Type Silicon Spreading Resistance Calibration

450

(100) p-Type Silicon Spreading Resistance Calibration

849

(111) n-Type Silicon Spreading Resistance Calibration

850

(111) p-Type Silicon

Stabilized Wine

Stainless Steel D803a Stainless Steel D807a Stainless Steel 803a Stainless Steel (AISI 446) 804a Stainless Steel (AISI 446) 805a Stainless Steel, 13% Chromium

807a Stainless Steel, Cr-Ni

808a Stainless Steel, Cr-Ni

809a Stainless Steel, Cr-Ni

817b Stainless Steel, Cr-Ni

820a Stainless Steel, Cr-Ni

821 Stainless Steel, Cr-Ni

827 Stainless Steel, Cr-Ni

436 Stainless Steel, Cr-Ni

437 Stainless Steel, Cr-Ni-Mo

438 Stainless Steel, Cr-Ni-Nb

439 Stainless Steel, Cr-Ni-Ti

440 Stainless Steel for Pitting or Crevice

441 Corrosion

837 Stainless Steel Thermal Expansion

840 Stearic Acid Rubber Compound

D837 Steel (AISI 1211)

Steel (Lead-Bearing)

D840 Strontium Cyclohexanebutyrate

Strontium-85 Radioactivity Standard

D841 Strontium-89 Radioactivity Standard

Styrene-butadiene Rubber (Type 1500) 625 Succinonitrile Freezing Point Sucrose 626 Sulfate and Nitrate on Filter Media

Sulfur Dioxide in Nitrogen

627 Sulfur Dioxide in Nitrogen

Sulfur Dioxide in Nitrogen 628 Sulfur Dioxide in Nitrogen

Sulfur Dioxide in Nitrogen 629 Sulfur Dioxide in Nitrogen

630

631

641 642

643

SRM

644

645 646

493 2529

2528

2527

2526

1590 121d

123c 160b

367

1267 73c CI 151 1151a CI 152 1152a CI 153 1153a C1154

1154a

1155 1172

1171 1890

738 372h

368 1169b

1070a 4403L-B

4945D

386h

1970

17c 2673

1661a

1662a

1663a

1664a

1693 1694

24
Name SRM Name SRM

Sulfur Dioxide in Nitrogen

Sulfur Dioxide Permeation Tube

(2 cm tube) Sulfur Dioxide Permeation Tube

(5 cm tube) Sulfur Dioxide Permeation Tube

(10 cm tube) Sulfur in Coal

Sulfur in Coal

Sulfur in Coal

Sulfur in Coal

Sulfur in Residual Fuel Oil Sulfur in Residual Fuel Oil Sulfur in Residual Fuel Oil Sulfur in Residual Fuel Oil Sulfur in Residual Fuel Oil Sulfur in Residual Fuel Oil Sulfur Rubber Compound

Superconductive Thermometric Fixed

Point Device

Superconductive Thermometric Fixed

Point Device

Surface Flammability Standard

Synthetic Sapphire

Technetium-99 Radioactivity Standard

Technetium-99m Radioactivity

Standard

Tetrachloroethylene in Nitrogen

Thallium-201 Radioactivity Standard

Thermal Resistance, Fibrous Glass

Batt Thermal Resistance, Fibrous Glass Board

Thorium-228, Thallium-208 Gamma-ray

Point-Source Standard

Tin-Base Bearing Metal

Tin, Freezing Point

Tin-1 13-Indium-l 13m Radioactivity

Standard

Tin- 121m Point-Source Gamma-ray

Emission-Rate Standard

Tin, Secondary Freezing Point Standard

Titanium Alloy

Titanium-Base Alloy

Titanium-Base Alloy

1696 Titanium-Base Alloy (Unalloyed) 650

Titj}niiim-Ra<;p Allov ^1 Jnallnvpfi^ 651 Titanium-Base Alloy (Unalloyed) 652

1676 Titanium Dioxide 154b Toluene 21 lc 167S Tomato Leaves 1573 Tool Steel (AISI M2) 132b Tool Steel (AISI M2) 1157 ZOO J Tool Steel Abrasive Wear Standard 1857

ZOo't TVarpflllov ^Niokpl-Rasp 897 ZOoD Hiph-Tpmnpraturp l l tL 11 I t-IIIUV.i UIU1 V 'Allov^ L I l\J J J 1619 Tracealloy (Nickel-Base 898

i ozua High-Temperature Alloy)

1 OZ 1 D Tracealloy (Nickel-Base 899

1 OZZD High-Temperature Alloy)

i oz ja Trace Elements in a Glass Matrix 610

1 A74-> Trace Elements in a Glass Matrix 611 n}7 1 n'g Trace Elements in a Glass Matrix 612

/Old Trace Elements in a Glass Matrix 613 Trace Elements in a Glass Matrix 614

/DO Trace Elements in a Glass Matrix 615 Trace Elements in a Glass Matrix 616

1UUZC Trace Elements in a Glass Matrix 617 770 Trace Elements in Coal (Bituminous) 1632a 4788 Trace Elements in Coal (Sub- 1635 fa a i nu i + iun-1 bituminous Trace Elements in Coal Fly Ash 1633a

1 808 QUO Trace Elements in Fuel Oil 1634a 44041 F Trace Elements in Water 1643a

1451 Trace Mercury in Coal 1630

2,2,4-Trimethylpentane 217c

1 4SOh Tripalmitin 1595

Tris, Basimetric 723a

470f>P Tris, for Solution Calorimetry 724a Tris(hydroxymethyl)aminomethane 922 S4H Tris(hydroxymethyl)aminomethane 923

741 hydrochloride

4402L-C Tris(l -phenyl- 1, 3-butanediono) 1078b Chromium (III) Tris(l -phenyl- 1, 3-butanediono) 1079b

Iron (III) 47p tzg Triphenyl Phosphate 1071b Tungsten Carbide 276a 654a Tungsten-Chromium-Vanadium Steel 50c 171h 1 / J\J Tungsten Concentrate 277

176 Tungsten, Heat Capacity 782 Tungsten-20% Molybdenum Alloy 480

Electron Microprobe Standard

Tungsten Thermal Expansion 737 Unalloyed Copper 1034 Unalloyed Copper, Cu "O" 393 Unalloyed Copper, Cu IV 457 Unalloyed Copper, Cu XI 454

Unalloyed Copper, Cu I (Chip) 394 Unalloyed Copper, Cu II (Chip) 395 Unalloyed Copper, Cu III (Chip) 396 Unalloyed Copper, Cu V (Chip) 398 Unalloyed Copper, Cu VI (Chip) 399 Unalloyed Copper, Cu VII (Chip) 400

Unalloyed Copper, Cu I (Rod) 494

25
Name SRM

Unalloyed Copper, Cu II (Rod) 495 Unalloyed Copper, Cu III (Rod) 496

Unalloyed Copper, Cu V (Rod) 498 Unalloyed Copper, Cu VI (Rod) 499 Unalloyed Copper, Cu VII (Rod) 500 Unalloyed Titanium 354 Uranium Isotopic Standard (Nominally U-0002

depleted to 0.02%)

Uranium Isotopic Standard U-005a

Uranium Isotopic Standard U-010

(Nominally 1% Enriched)

Uranium Isotopic Standard U-015

(Nominally 1.5% Enriched)

Uranium Isotopic Standard U-020

Uranium Isotopic Standard U-030a

Uranium Isotopic Standard U-050

(Nominally 5% Enriched)

Uranium Isotopic Standard U-100

(Nominally 10% Enriched)

Uranium Isotopic Standard U-150

(Nominally 15% Enriched)

Uranium Isotopic Standard U-200

(Nominally 20% Enriched)

Uranium Isotopic Standard U-350

(Nominally 35% Enriched)

Uranium Isotopic Standard U-500

(Nominally 50% Enriched)

Uranium Isotopic Standard U-750

(Nominally 75% Enriched)

Uranium Isotopic Standard U-800

(Nominally 80% Enriched)

Uranium Isotopic Standard U-850

(Nominally 85% Enriched)

Uranium Isotopic Standard U-900

(Nominally 90% Enriched)

Uranium Isotopic Standard U-930

(Nominally 93% Enriched)

Uranium Isotopic Standard U-970

(Nominally 97% Enriched)

Uranium Metal 960

Uranium Oxide 950b

Uranium Oxide 969 Uranium-233 Spike Assay and 995

Isotopic Solution Standard

Uranium-235 Spike Assay and 993

Isotopic Solution Standard

Urban Dust/Organics 1649 Urban Particulate Matter 1648 Urea 912a Urea 2141 Urea 2152

Uric Acid 913 Vanadium and Nickel in Residual 1618 Fuel Oil Vanadium in Curde Oil 8505 Vanadium-49 Low-Energy Photon 4266

Standard

Waspaloy 349 Wear-Metals in Lubricating Oil 1084

(100 ppm)

Name SRM

Wear-Metals in Lubricating Oil 1085

(300 ppm)

Wheat Flour 1567 White Cast Iron 338 White Cast Iron (Disc) 1 145 White Cast Iron (Disc) 1 146 White Cast Iron (Disc) 1 1 50 White Ceramic Tile for Directional 2019b

Hemispherical Reflectance White Ceramic Tile for Directional 2020

Hemispherical Reflectance White Iron 3d

White Opan Glass Diffuse Spectral 2015

Reflectance Standard for the

Visible Spectrum

Xenon- 127 Gaseous Radioactivity 4309G

Standard

Xenon-133 Gaseous Radioactivity 43071 Standard

Xenon-133 Gaseous Radioactivity 4415L-I Standard

Xenon-133, Xenon-137, Krypton-85 4310B

Mixed Gaseous Radioactivity

Standard

X-ray Film Step Tablet 1001 X-ray Powder Diffraction Intensity 674

Standard

X-ray Powder Diffraction (Mica) 675 Low 2 Theta

Ytterbium- 169 Radioactivity Standard 4419L-B

Zinc-Base Alloy (Die Casting) 94c Zinc Concentrates 113a Zinc Concentrates 329 Zinc Cyclohexanebutyrate 1073b

Zinc, Freezing Point 740

Zinc, Freezing Point Standard 43h

Zinc Metal 683 Zinc Oxide Rubber Compound 370e

Zircaloy-2 360a

Zircaloy-4 Metal 1237

Zircaloy-4 Metal 1238 Zircaloy-4 Metal 1239 Zirconium-Barium Chromate 1651 Formulation for Heat-Source Powder Calorimetry

Zirconium-Barium Chromate 1652 Formulation for Heat-Source Powder Calorimetry

Zirconium-Barium Chromate 1653 Formulation for Heat-Source Powder Calorimetry

Zirconium Metal 1234 Zirconium Metal 1235 Zirconium Metal 1236

26
U.S. Department of Commerce

• luaniuiH. Krepa

Secretary Appendix II. Certificates for Biological and Botanical

Standards (listed in numerical order).

National Bu«*U of Standard! EmeM Ambler, Acting Director Rational ^nttmx of Standards

^Report of (Slrtbegtigatton

Research Material 50

Albacore Tuna

P. D. LaFleur and W. P. Reed

A lyophilized (freeze-dried) marine biological tissue sample has been prepared in an attempt to satisfy many of

the analytical requirements for a base line marine reference material. Tuna fish muscle tissue was chosen for this purpose because of availability, use as a fish foodstuff, and its oceanographic interest. The tuna is available as a Research Material (RM), in sets of two cans. Each can contains approximately 35 grams of lyophilized tuna tissue in a polyethylene bag, inside the hermetically-sealed, nitrogen filled can.

Material Application

This particular RM is intended to be used in the measurement of elements present at trace concentrations. In addition, some measurements of trace hydrocarbons have been made. The material represents a typical marine

tissue that has been lyophilized. It should prove useful to those scientists who may wish to evaluate analytical methods, or to use a generally available "real" sample matrix in interlaboratory comparisons. It is important to note, however, that this is not a Standard Reference Material and none of the data presented here are certified. For the convenience of the analyst using this material, we have included a discussion for each component

reported. It is apparent that there is significant heterogeneity for some elements, specifically the "bone-seeking"

elements. The homogeneity of other elements appears to be acceptable. In most instances the heterogeneity of

the material is observed between samples from the same can. There is some evidence that this heterogeneity is due primarily to fine particles of cartilage or bone present with the tissue in this RM.

Material Preparation The tuna tissue used in this research material is from albacore tuna caught in the San Diego area in July of 1971. The tuna was cleaned, filleted, frozen and transported to a lyophilization facility. It was thawed, ground, and mixed using stainless steel equipment. For the final mixing, the entire lot was held in a single stainless steel container. The tuna was then lyophilized in aluminum trays lined with polyethylene. After lyophilization, the material was again ground in a stainless-steel mill, carefully transferred to new, individual polyethylene bags, and canned under nitrogen for storage.

Preliminary studies performed on these canned samples raised serious problems about the homogeneity of the

material. In an effort to improve this condition the material was reground, reblended and recanned under the same conditions, as before. Except where stated, all measurements provided in this report were made on the reprocessed material.. During the second regrinding, fibrous material tended to "float" to the top due to the action of the mixer. This material was discarded.

(over)
Material Use The lyophilized tuna tissue, sealed in metal cans, should have an indefinite storage life under normal room conditions. Thus far, no evidence exists to indicate deterioration of the material with time as long as the can remains

sealed. Once opened, the possibility exists that the raw material will turn rancid. Researchers using only a

portion of the material have successfully stored the remainder by placing the polyethylene bag in a glass jar with

lid and storing at or below 0°C. A shelf life of 6 months is not unusual under these circumstances and a 2 year

stability has been reported. The freeze-dried material represents only about 30% of the original weight of the tuna tissue. Consequently, for direct comparisons with fresh tissue the researcher may wish to adjust the sample

weight for this difference. Please note, however, that all values in this "Report of Investigation" are based on the

lyophilized weight of the tuna tissue. The dissolution of this material is not difficult and researchers have reported using a variety of techniques. These techniques include nitric and perchloric acid digestion, nitric-sulfuric-perchloric (4:1:1) acid digestion, low temperature ashing, and oxygen combustion. Because of the apparent presence of small bits of cartilage and/or bone in the sample, the sample size for analysis should be 250 mg or greater to obtain reproducible

results. For "bone-seeking" elements (e.g., Pb, U, Ca, Sr) the sample may be nonhomogeneous even with much

larger sample sizes.

For the elements listed below, sufficient analytical work has been performed to permit some evaluation of the

data. These brief evaluations are not certified values, but only judgments as to the amount of the element

present. Problems encountered by analysts making these measurements are also described.

Mercury The question of the mercury content of various foodstuffs has been studied by many investigators It is hoped

that this material will provide a base line RM for environmental studies of mercury in food. A value of 0.95 ± 0.

1

ppm encompasses the means of all of the reported values for mercury with one exception and extensive studies have indicated good homogeneity for this element. Agreement among methods appears to be good. The data show very little bias among the three methods used. Sample sizes for these measurements have been 0.25 grams and larger. The question of volatile mercury was explored by one investigator who reported the mercury content decreasing

after opening the container. This decrease amounted to approximately 0. 1 ppm over a period of 3 weeks after opening even though stored at -25 °C. This work has not been confirmed. Further work by several investi- gators has suggested that 80-90% of the mercury content is present as methylmercury.

Selenium

This element was determined by four laboratories using both neutron activation analysis and atomic absorption

spectroscopy. The range of reported values (x) is from 3 27 to 4.01 ppm. A most probable value is 3.6 ± 0.4. There has been very little indication of heterogeneity for this element

Zinc The analysis for zinc was performed by three laboratories using two analytical methods, neutron activation

analysis and atomic absorption analysis. The range of mean values (X) is 1 1 4 to I4.6ppm. Our estimate of the probable value is 13.6 ± I ppm. No random behavior has been noted in the analysis ol this element.

(Aj25|jjc- The analysis for arsenic was performed by 4 laboratories using both neutron activation and atomic absorption

techniques. Data obtained within each laboratory appears to be consistent, although there appears to be some

disagreement among laboratories analyzing for this element. I he range of average values (x) between laboratories is 2.74 to 4.6 ppm. The recommended value for the arsenic content is 3 3 + 0 4 ppm

28
Lead

There is a limited amount of data available for lead. The average value is 0.46 ppm. The homogeneity of the material for this element is questionable and the range of individual values is quite large. The use of this Research Material as a control, or for the development of methods for lead cannot be recommended. The isotopic composition, however, has been determined and is: 20, Pb 52.2%;

20, Pb 21.5%;1MPb 24.9%; and JMPb 1.38%

Other Elements Manganese was found to be distributed homogeneously and the measured value in the original lot of tuna (not the reblended material) was 1.3 ppm. Sodium also was found to be homogeneous in the original lot of material and the reported value was 0.1 1%. Potassium was found to be distributed homogeneously and the measured value on the original lot of tuna (not the reblended material) was 1.22%. The following elements have been found to be heterogeneously distributed

in the tuna tissue: uranium, thorium, calcium, and strontium.

Organic Materials The tuna research material was subjected to headspace sampling and analysis. The concentrations given in the following table are to be considered only as order-of-magnitude estimates of the hydrocarbon compounds

present, as they were calculated relative to the amount of naphthalene added to the sample and most of the compounds identified were not aromatic hydrocarbons. The value for the largest constituent, 2.6-di-t-butyl-p-cresol, is an especially poor estimate. However, this compound is interesting because it is a common antioxidant used in food packaging. It probably has its origins

in one of the handling steps between tuna collection and packaging in plastic. An indication of petroleum pollution in the tuna sample comes from the aliphatic hydrocarbons present. The identification of the monoterpene. limonene, is reasonably certain. As with pristane this compound is composed of isoprene units, but limonene is generally considered to be a product of plant biosynthesis. Its origin in this sample is uncertain, but it may arise from plant material ingested by tuna.

Identification and Approximate Quantitation of Major Isolated Organic Constituents

Compound Identification* Amount Present (ppm)

Heptadiene (?) 0.6 Toluene 0.7 limonene 0.4 2-nonunone ('.') 0.7 2-undccanonc (?) 0.

1

2.6-di-t-butyl-p-cresol 1.0 llexadecane trace Hcptadecanc trace Pristane 0.03

•Identification followed by a (?) is probable but not definite.

Summary

I he analytical values presented in this report represent the authors' evaluation of a considerable amount of

data, lor many elements where t he reports were inconclusive, probable values are not given. If more information becomes available in the future, this report will he updated. Your help in reporting data will be appreciated.

Send any reports to:

Office ol Standard Reference Materials (R-701)

H3I6. C hemistry National Bureau of Standards Washington. DC. 20234 May 12. 1977

29
U. S. Department 'of Commerce

MalcolnrBaldrigc Secretary

National Bureau brStandards

Ernesi Amottr. Director Rational ^mmn of j^tandartfe

Certificate of

Standard Reference Material 1549

Non-Fat Milk Powder

This Standard Reference Material (SRM) is intended primarily for use in calibrating instrumentation and evaluating the

reliability of analytical methods for the determination of constituents in milk, milk powders, and other biological

matrices.

Certified Values of Constituents: The certified concentrations of the constituent elements are shown in Table 1. Certified values are based on results obtained by definitive methods of known accuracy; or alternatively, from concordant results by two or more independent analytical methods.

Additional Information on Composition: Noncertified concentrations of additional constituent elements are given for information only in Table 2. Noncertified concentrations of lactose and ascorbic acid were determined by high

performance liquid chromatography; and for lactose only, by nuclear magnetic resonance.

Notice and Warnings to Users:

Expiration of Certification: This certification is invalid after 3 years from the date of shipping. Should it become

invalid before then, purchasers will be notified by NBS.

Stability: The material should be kept in its original bottle and stored at temperatures between 10- 30 'C. It should net '>e exposed to intense sources of radiaiion. The bottle should be kept tightly closed and stored in a desiccator in the dark.

Use: A minimum sample of 500 mg of the dried material (see Instructions for Drying) should be used for any analytical determination to be related to the certified values of this certificate.

Dissolution procedures should be designed to effect complete dissolution, but without losses of volatile elements, such as mercury. Dissolution for these determinations should be carried out in a closed system.

Statistical consultation was provided by K.R Ebcrhardt of ihe Statistical Engineering Divis'on. Thj o/erall direction and cocrdinatioi of the analyses were under the chairmanship of E.L. Garner, Chief of the Inorganic Analytical Research Division, and W.E. May, Chief of the Organic Analytical Research Division. The technical and support aspects involved in the preparation, certification, and issuance of this Standard Reference

Material were coordinated through the Office of Standard Reference Materials by R. Alvarez.

Gaithersburg, MD 20899 Stanley D. Rasberry, Chief

July 29, 1985 Office of Standard Reference Materials (Revision of Certificates (over) dated 4-17-84 and

1-14-85)

30
Instructions for Drying Samples of this SRM must be dried before weighing according to the following procedure: Dry

for 48 hours at 20 to 25 °C in a vacuum oven at a pressure not greater than 30 Pa (0.2 mm Hg).

Analysts:

Center for Analytical Chemistry, National Bureau of Standards:

1. E.S. Beary

2. J.M. Brown-Thomas

3. T.A. Butler

4. B. Coxon

5. M.S. Epstein

6. J.D. Fassett

7. J.W. Gramlich

8. R.R. Greenberg

9. W.R. Kelly

10. H.M. Kingston

11. W.F. Koch

12. G.M. Lambert

13. G.J. Lutz

14. J.R. Moody

15. T.J. Murphy

16. P.J. Paulsen

17. T.C. Rains

18. T.A. Rush

19. M.E. Watson

20. R.L. Walters, Jr.

21. L. Watts

Cooperating Analysts:

22. R.W. Dabeka, Food Research Division, Health Protection Branch, Tunney's Pasture, Ottawa, Ontario, Canada.

23. L. Kosta, A.R. Byrne, M. Dermelj, Institute "Josef Stefan", Ljubljana, Yugoslavia.

24. C. Veillon and K. Patterson, Beltsville Human Nutrition Research Center, U.S. Department of Agriculture,

Beltsville, MD.

Element

Table 1. Certified Concentrations of Constituent Elements

Calcium

Chlorine

3 ' 5 '

Magnesium2 '

Phosphorus

Element ~ . • lb. 5b Cadmium

Chromium4c 5b

r, lb,2i.5b Copper

. ,. 4l.6 Iodine

. 4«,5l Iron

Concentration,

weight, %

1.30

1.09 0.120

1.06

Element ±0.05 ± .02 ± .003 ± .02

Concentration, Mg/g

0.0005 + 0.0002

.0026 ± .0007

.7

3.38

1.78

Potassium

Sodiura

2c ' 5

Sulfur

3

' 4*

Element

2b. 5a

lb. 4a

lb. 2a. 5a

.1

.02

.10

Lead Manganese w la. 5b Mercury

. Id. 4b. 5a. 5b Selenium

1. Atomic absorption spectrometry

a. cold vapor

b. electrothermal

c. flame

d. hydride generation

2. Atomic emission spectrometry

a. dc nlasma

b. flame

c. inductively coupled plasma

3. Ion chromatography

Zinc

4

lc. 2c. 4b. 5a

Concentration,

weight, %

169 ±0.03

0.497 ± .010

.351 ± .005

Concentration, Mg/g

0.019 ±0.003

26 ± .06 .0003 ± .0002

11 ± .01

46.1 ±2.2

Isotope dilution mass spectrometry

a. thermal ionization

b. spark source

c. electron impact

5. Neutron activation

a. instrumental

b. radiochemical

6. Photon Activation

Notes: (1.) Analytical values are based on the "dry-weight" of material (see Instructions for Drying)

(2.) Except for Fe, the stated uncertainty includes the union of 95% confidence intervals computed separately for each analytical method. It includes the effects of measurement

error, possible effects of known systematic errors, and between-method differences The

uncertainty for Fe h f^ven as a 95% confidence interval for the weighted mean of the mass

spectrometric and neutron activation values, and includes an allowance (added linearly) for systematic error in the methods. The weights were chosen to minimize the estimated mean squared error of the weighted mean, as described in "Approximately Linear Models," by J. Sacks am' D. Ylvisaker, Annals of Statistics 6. pp. 1122-1137, 1978.

31
Table 2. Noncertified Concentratio ns of Constituent Elements

Element Aluminum

Antimony

Arsenic Bromine

Cobalt

Fluorine

Concentration, Mg/g

( 2 )

( 0.00027)

( .0019 ) (12 )

( 0.0041 )

( -20 )

Element Molybdenum

Rubidium

Silicon

Silver Tin

Concentration, Mg/g

( 0.34 )

( U ) «50 )

( <0.0003)

( <0.02 )

Table 3. Noncertified Concentrations of Organic Constituents

Compound

Lactose

Compound

Ascorbic Acid

Number of Determinations

Number of Determinations

10

Concentration,* weight %

49 ± 3

45 ± 2

Concentration, Mg/g

53 ± 5

Method

High Performance

Liquid Chromatography

Proton Nuclear Magnetic Resonance

Methods

High Performance

Liquid Chromatography

Uncertainties represent one standard deviation.

32
U. S. Depart*rtt*f Commerce MBlcolJMialdriae

Rational ££uram of j^tartdartts

Certificate of (Analysts

Standard Reference Material 1566

Oyster Tissue

This Standard Reference Material is intended primarily for use in calibrating instrumentation and validating methodology for the chemical analysis of marine animal tissue.

Certified Values of Constituent Elements: The certified values for the constituent elements are shown in Table I

.

Certified values are based on results obtained by reference methods of known accuracy; or alternatively, from

results obtained by two or more independent and reliable analytical methods. Non-certified values are given for information only in Table 2. All values are based on a minimum sample size of 250 mg of the dried material. NOTICE AND WARNINGS TO USERS

Expiration of Certification; This certification is invalid after 5 years from the date of shipping. Should it become

invalid before then, purchasers will be notified by NBS.

Storage: The material should be kept tightly closed in its original bottle and stored in a desiccator at temperatures between 10-30° C. It should not be exposed to intense sources of radiation, including ultraviolet lamps or

sunlight.

Use: A minimum sample weight of 250 mg of the dried material (see Instructions for Drying) is necessary for any certified value in Table 1 to be valid within the stated uncertainty. The bottle should be shaken well before each use, and closed tightly immediately after use. The statistical analysis of the data was performed by K. R. Eberhardt and H. H. Ku of the Statistical Engineering Division. The overall direction and coordination of the analytical chemistry measurements leading to this certificate were performed in the NBS Center lor Analytical Chemistry by P. D. LaFleur.

The technical and support aspects involved in the preparation, certification, and issuance of t his Standard

Reference Material were coordinated through the Office of Standard Reference Materials by R. Alvarez.

Washington, D.C. 20234 George A. Uriano, Chief Kebruary 22, 1983 Office of Standard Relerence Materials (Revision of Certificate Dated 12-12-79)

(over)

33
Instructions for Drying: Before weighing, samples of SRM 1 566 should be dried to constant weight by one of

the following procedures:

1. Reduced-pressure drying at room temperature for 48 hours over MglClftih in a vacuum desiccator at approximately 1.3 x 10 Pa (100 mm Hg).

2. Vacuum drying at room temperature for 24 hours at a pressure of approximately 30 Pa (0.2 mm Hg)

using a cold trap.

3. Freeze drying for 20 hours at a pressure of approximately 3 Pa (0.02 mm Hg).

Source and Preparation of Material: The oysters lor this reference material were obtained by the FDA Bureau

of Shellfish Sanitation from a commercial source. They had been shucked, frozen, and packaged in sealed

plastic bags. The oyster material was ground, freeze-dried, and powdered at the U.S. Army Natick Research and

Development Command, Natick, Mass., under the direction of L. Hinnegardt and G. C. Walker. At NBS,

preliminary analyses of the material homogeneity indicated that an improvement in homogeneity would be

required to establish more reliable certified values for a minimum sample size of 250 mg. Accordingly, the material was cryogenically ground by J. R. Moody and J. Matwey. It was then blended and bottled at NBS, after which it was again freeze-dried at the Natick, Mass., laboratory.

Homogeneity Assessment: Randomly selected bottles of SRM 1 566 were sampled and tested for homogeneity

by neutron activation and atomic absorption spectrometry. No inhomogeneity was observed for the following elements determined by neutron activation: Na, CI, V, and Mn. The values for Mg, K, Cu, Zn, and Cd

determined by atomic absorption spectrometry were within the imprecision of the method; however, Ca does

exhibit some inhomogeneity-approximately 4% relative standard deviation.

Analysts:

Center for Analytical Chemistry, National Bureau of Standards:

1. J. V. Bailey 17. W. R. Kelly

2. C. Blundell 18. H. M. Kingston

3. T. J. Brady 19. W. F. Koch

4. M. Diaz 20. R. M. Lindstrom

5. L. P. Dunstan 21. G. J. Lutz

6. M. S. Epstein 22. L. A. Machlan

7. J. D. Fassett 23. W. A. MacCrehan

8. M. Gallorini 24. E. J. Maienthal

9. E. L. Garner 25. J. Maples

10 T. E. Gills 26. O. Menis

1

1

J. W. Gramlich 27. J. D. Messman

12. R. R. Greenberg 28. J. R. Moody

13. S. Hanamura 29. L. J. Moore

14 S. Harrison 30. T. J. Murphy

15. E. F. Heald 31. P. .1. Paulsen

16. G. M. Hyde 32. T. C. Rains

33. H. L. Rook

Cooperating Analysts:

34. University of Tokyo, Tokyo, Japan; Y. Dokiya (NBS Guest Worker).

35. Division of Chemistry, National Research Council of Canada, Ottawa, Canada; S. Berman,

A. Desaulniers, J. McLaren, A. Mykytiuk, D. Russell, and S. Willie.

36 Ibaraki Electrical Communication Laboratory, Nippon Telegraph and Telephone Public Corporation,

Tokai, Ibaraki, Japan; K. Kudo and K. Kobayashi.

37. Food Research Division, Health Protection Branch, Tunney's Pasture, Ottawa, Ontario, Canada;

R. W. Dabtka, A. D. McKenzie. and H. B. S. Conacher.

34
Element Calcium

,d

Magnesium'

Element

1

Arsenic

,f,«,h

Cadmium

Chromium

Copper'

1 ''""'

. b.c.c.r Iron

. .a.d.e.h Lead Manganese

1*' 1

Mercury

Table I. Certified Values of Constituent Elements

Element'

Potassium Sodium6 ''

Element'

Nickel*'""

Rubidium*

1 '

Selenium'''''

Silver"

,f

„ b,<l Strontium Uranium Vanadium —. a,c,d,e,r,h Zinc

Content . Wt, Percent

0.15 ± 0.02

0.128 ± 0.009

Content

2

, /ig/g

13.4 ± 1.9

3.5 ± 0.4

0.69 ± 0.27

63.0 ± 3.5

195 ± 34

0.48 ± 0.04

17.5 ± 1.2

0.057 + 0.015

Content . Wt. Percent

0.969 ± 0.005

0.51 ± 0.03

Content

2

, /ig/g

1.03 ± 0.19

4.45 ± 0.09

2.1 ± 0.5

0.89 ± 0.09

10.36 ± 0.56 0.1I6± 0.006

2.3 ± 0.1

852 ± 14

1. Analytical Methods:

Atomic absorption spectroscopy Atomic emission spectroscopy, flame

1 Atomic emission spectroscopy.

inductively coupled plasma

J,Isotope dilution mass spectrometry. thermal ionization

' Isotope dilution mass spectrometry, spark source

1 Neutron activation

f Photon activation Polarography

2. Based on dry weight. (For drying instructions, see the section of this certificate on Instructions for Drying.) The estimated uncertainty is given as 95 percent tolerance limits for coverage of at least 95 percent of the measured values of all bottles of SRM 1566. For a given element, the following statement can be made at a confidence limit of 95 percent. "If the concentrations were measured for all bottles, at least 95 percent of these measured values should fall within the indicated limits." The concept of tolerance limits is discussed in Chapter 2, Experimental Statistics, NBS Handbook 91, 1966, and page 14, The Role of Standard Reference

Materials in Measurement Systems, NBS Monograph 148, 1975.

Table 2. Non-certified Values of Constituent Elements

Content'

(Wt. Percent)

(1.0)

Element

Chlorine

Sulfur Phosphorous

Bromine

Cobalt

Fluorine

Iodine Molybdenum

Thallium Thorium

(0.76)

(0.81)

(/Jg/g)

(55)

(0.4)

(5.2)

(2.8)

(<j)2)

(<0.005)

(0.1)

Based on dry weight. (For drying instructions, see the section ol

this cerlilicate on Instructions lor Drying.)

35
U.S. Department of Commerce

Juanita M Kreps

Secretary

National Birtw\j of Standards

(Smesl Ambler, Acling Pirwtor

Rational ££itram of j^tandarcta

Certificate of (Analgste

Standard Reference Material 1567

Wheat Flour

This Standard Reference Material is intended primarily for calibrating instrumentation and evaluating the

reliability of analytical methods for the determination of minor and trace elements in wheat flour and similar agricultural food products.

Certified Values of Constituent Elements: The certified values for the constituent elements are shown in Table 1

.

They are based on results obtained by two or more independent, reliable analytical methods. Non-certified values which are given for information only, appear in Table 2.

All values are based on a minimum sample size of 400 mg and are reported on a "dry-weight" basis.

Notice and Warnings to Users:

Expiration of Certification: This certification will be invalid after 5 years from the date of shipping. Should it be

invalidated before then, purchasers will be notified by NBS.

Storage: The material should be kept in its original bottle and stored at temperatures between 10-30 °C. It should not be exposed to intense sources of radiation, including ultraviolet lamps or sunlight. Ideally, the bottle should be kept in a desiccator in the dark at the temperature indicated.

Use: The following procedures should be followed to relate the analytical determinations to the values reported

in this Certificate. The bottle should be shaken well before each use, and a minimum sample of 400 mg of the material should be used. Selenium and mercury should be determined in the material without drying and the concentration values adjusted for the moisture content of the material using separate samples. Other elements may be determined either on samples without drying as indicated above or on samples vacuum-dried for 24 hr as indicated under "Instructions for Drying." The overall direction and coordination of the technical measurements leadingto thisCertificatcwcre performed under the chairmanship of H. L. Rook. The technical and support aspects involved in the preparation, certification, and issuance ol this Standard

Reference Material were coordinated through the Office of Standard Reference Materials by R. Alvarez.

Washington, D C. 20234 J. Paul Cali, Chief January 3, 1978 Office of Standard Reference Materials

(over)

36
Table I . Certified Values of Constituent Elements

3

Minor Constituents

Element

Potassium Calcium

Trace Constituents

Element

Iron Zinc Manganese

Sodium

Content

18.3 ± 1.0 10.6 ± 1.0 8.5 ±0.5

8.0 ± 1.5

Content

Wt. Percent

0.136 ± 0.004

b

0.019 ± 0.001

Element Copper

Selenium Cadmium

Mercury

Content

2.0 ± 0.3

1.1 ±0.2

0.032 ± 0.007

0.001 ± 0.0008

Analytical values are based on the "dry-weight" of material (see Instructions for Drying). Selenium and mercury should be deter- mined on samples without drying and the results adjusted to a "dry-weight" basis by determining moisture on separate samples The estimated uncertainty is based on judgment and represents an evaluation of the combined effects of method imprecision,

possible systematic errors among methods, and material variability for samples 400 mg or more. (No attempt was made to derive exact statistical measures of imprecision because several methods were involved in the determination of the constituents).

Table 2. Non-certified Values for Constit uent Elements

3

NOTE: The values shown in this table are not certified because they are not based on the results of two or more

independent reliable methods. These values are included for information only.

Trace Constituents

Element Bromine Rubidium Molybdenum

Content Mg/g Element

Nickel Arsenic Tellurium

Content Mg/g

(0.18) (0.006) (<0.002)

Analytical values arc bused on the "dry-weight" of material {see Instructions for Drying).

37
Preparation of Material: The wheat flour for this Standard Reference Material was described by the supplier

as milled from a blend of Hard Red Spring and Hard Red Winter wheat grown primarily in South Dakota. The

flour was taken from the mill packer during the middle of a run to obtain homogeneous material. The flour had

been bleached and brominated in accordance with standard treatments for commercial bakery use. At NBS,

the material was passed through a sieve with openings of 425 ixm (No. 40) and blended. The bottled material was

then subjected to 2.5 megarads of Co-60 radiation for microbiological control at Neutron Products, Inc., Dickerson, Md.

Homogeneity Assessment: A preliminary evaluation of homogeneity was made by instrumental neutron activa- tion using samples of 1 50 to 300 mg and counting the activities from radionuclides of Mn, K, Zn, Na, and Br. The homogeneity of other certified elements was evaluated using samples of 400 mg or less with the exception of mercury and calcium for which 500 mg and 1 g, respectively, were used. The uncertainties for the concentrations

in Table 1 include these results.

Instructions for Drying: Except for selenium and mercury, elements may be determined on samples which have

been dried as follows: Vacuum-dry the material at approximately 25 °C for 24 hours at a pressure not greater than 70 Pa (0.5 mm Hg)

with a cold trap at a temperature of about —30 °C or below.

Se and Hg should be determined on undried samples: other elements may be so determined. However, because

the Certificate values are reported on a "dry-weight" basis, the elemental concentrations determined on undried samples should be adjusted for the moisture content of the samples. The moisture content, which was

approximately 9% when bottled, should be determined on separate samples by either the vacuum-drying

procedure described above or drying the sample in air inanovenat 85°Cfor 24 hours. Both of these procedures

yielded the same loss in weight. Samples for analysis should not be oven-dried lest elements be lost by volatilization.

Analytical Methods Used and Analysts

Analytical Methods

A. Atomic absorption spectrometry

B. Flame emission spectrometry

C. Isotope dilution spark source mass spectrometry

D. Neutron activation

E. Polarography

Analysts

Analytical Chemistry Division, National Bureau of Standards

9. R. M. Lindstrom

Cooperating Analysts

IS. W. R Wolf and J. Holden, Nutrition Institute, U.S. Department of Agriculture, Beltsville, Md.

1. J. R. Baldwin

2. 1. J. Brady

10. G. J. Lutz

1 1. E. J. Maienthal

12. R. Mavrodineanu

13. J. D. Messman

14. R. M. Morris

15. P. J. Paulsen

16. T. C. Rains

17. P. A. Sleeth

3. M. G. Diaz

4 L. P. Dunstan

5. M. S. Epstein

6. M. Gallorini

7. T. E. Gills

8. R. R. Grecnberg

38
U- S. Department of Commerce

Malcolm fialdrige Secretary

'

! *>r"-V

National Buratt oTSlandards

Ernesi 'Ambfct. Cirecior

Addendum to

Rational £?ttram of j^tantlartfe

(Certificate of (Analgsi*

Standard Reference Material 1567

Wheat Flour

Additional Certification The following certified value is to be added to Table I.

Table l. Certified Values of Constituent Elements

2

Element Content, ng/g Lead 0.020 ±0.0 1

0

h

Lead should be delermined on samples without drying and the results adjusted to a "dry-weight" basis by determining moisture on separate samples The estimated uncertainty, based on judgment, is for samples 2 g or more.

Analytical Methods Used and Analysts

Inorganic Analytical Research Division, National Bureau ol Standards.

Isotope dilution, mass spectrometry, I. L. Barnes and E. S. Beary; Polarography, E. J. Maienthal.

Cooperating Analyst

R. W. Dabeka, Food Directorate, Health Protection Branch, Ottawa, Canada.

39
U.S. Departm^^af Commerce

Notional Bufttfto oi Standards Emest Angler,' Acring Director ^aiiimal ^Bureau of jitawianta

(Certificate of JWalggis

Standard Reference Material 1568

Rice Flour

This Standard Reference Material is intended primarily for calibrating instrumentation and evaluating the

reliability of analytical methods for the determination of minor and trace elements in rice flour and similar agricultural food products.

Certified Values of Constituent Elements: The certified values for the constituent elements are shown in Table I

.

They are based on results obtained by two or more independent, reliable analytical methods. Non-certified values which are given for information only, appear in Table 2.

All values are based on a minimum sample size of 400 mg and are reported on a "dry-weight" basis.

Notice and Warnings to Users:

Expiration of Certification: This certification will be invalid after 5 years from the date of shipping. Should it be

invalidated before then, purchasers will be notified by NBS.

Storage: The material should be kept in its original bottle and stored at temperatures between 10-30 °C. It should not be exposed to intense sources of radiation, including ultraviolet lamps or sunlight. Ideally, the bottle should be kept in a desiccator in the dark at the temperature indicated.

Use: The following procedures should be followed to relate the analytical determinations to the values reported

in this Certificate. The bottle should be shaken well before each use, and a minimum sample of 400 mg of the material should be used. Selenium and mercury should be determined in the material without drying and the concentration values adjusted for the moisture content of the material using separate samples. Other elements may be determined either on samples without drying as indicated above or on samples vacuum-dried for 24 hr as indicated under "Instructions for Drying." The overall direction and coordination of the technical measurements leading to this Certificate were performed under the chairmanship of H. L. Rook. The technical and support aspects involved in the preparation, certification, and issuance of this Standard

Reference Material were coordinated through the Office of Standard Reference Materials by R Alvarez.

Washington, D.C. 20234 .1. Paul C all. Chief January 3, 1978 Office oi Standard Reference Materials

(over)

40
Table 1 . Certified Values of Constituent Elements

3

Minor Constituents

Element

Potassium Calcium

Trace Constituents

Element Manganese

Zinc

Iron Sodium

Copper

Content Mg/g

20. 1 ± 0.4

19.4 ± 1.0 8.7 ± 0.6

6.0 ± 1.5 2.2 ± 0.3

Content

Wt. Percent

0.112 ± 0.002

b

0.014 ± 0.002

Element

Arsenic Selenium Cadmium

Cobalt Mercury

Content Mg/g

0.41 ±0.05

0.4 ±0.1

0.029 ± 0.004

0.02 ±0.01

0.0060 ± 0.0007

Analytical values are based on the "dry-weight" of material (see Instructions for Drying). Selenium and mercury should be deter- mined on samples without drying and the results adjusted to a "dry-weight" basis by determining moisture on separate samples. The estimated uncertainty is based on judgment and represents an evaluation of the combined effects of method imprecision,

possible systematic errors among methods, and material variability for samples 400 mg or more. (No attempt was made to derive exact statistical measures of imprecision because several methods were involved in the determination of the constituents).

Table 2. Non-certified Values for Constituent Elements* NOTE: The values shown in this table are not certified because they are not based on the results of two or more

independent reliable methods. These values are included for information only.

Trace Constituents

Element Kubidium Molybdenum

Bromine

Content Mg/g

(7) (1.6)

(1)

Element

Nickel Tellurium

Content Mg/g

(0.16) (^.0.002)

Analytical values art- based on the "dry-weight" o! material (see Instructions lor Drying).

41
Preparation of Material: The rice flour for this Standard Reference Material was described by the supplier as 100% long grain from Arkansas. At NBS, the material was passed through a sieve with openings of 425 urn

(No. 40) and blended. The bottled material was then subjected to 2.5 megarads of Co-60 radiation for microbiological control at Neutron Products, Inc., Dickerson, Md.

Homogeneity Assessment: A preliminary evaluation of homogeneity was made by instrumental neutron activa- tion using samples of 1 50 to 300 mg and counting the activities from radionuclides of Mn, K, Zn, Na, and Br. The homogeneity of other certified elements was evaluated using samples of 400 mg or less with the exception of mercury and calcium for which 500 mg and 1 g, respectively, were used. The uncertainties for the concentrations

in Table 1 include these results.

Instructions for Drying : Except for selenium and mercury, elements may be determined on samples which have

been dried as follows: Vacuum-dry the material at approximately 25 °C for 24 hours at a pressure not greater than 70 Pa (0.5 mm Hg)

with a cold trap at a temperature of about -30 °C or below.

Se and Hg should be determined on undried samples; other elements may be so determined. However, because

the Certificate values are reported on a "dry-weight" basis, the elemental concentrations determined on undried samples should be adjusted for the moisture content of the samples. The moisture content, which was

approximately 9% when bottled, should be determined on separate samples by either the vacuum-drying

procedure described above or drying the sample in air in an oven at 85 °C for 24 hours. Both of these procedures

yielded the same loss in weight. Samples for analysis should not be oven-dried lest elements be lost by volatilization.

Analytical Methods Used and Analysts

Analytical Methods

A. Atomic absorption spectrometry

B. Flame emission spectrometry

C. Isotope dilution spark source mass spectrometry

D. Neutron activation

E. 1'oldi ography

Analysts

Analytical Chemistry Division, National Bureau of Standards

J. R. Baldwin T J. Brady M. G. Diaz

L. P. Dunstan M. S. Epstein M. Gallorini

7. T. E. Gills

8. R. R. Greenberg

9. R. M. Lindstrom

10. G. J. Lutz

11. E. J. Maienthal

12. R. Mavrodineanu

13. J. D. Messman

14. R. M. Morris

15. P. J. Paulsen

16. T. C. Rains

17. P. A. Sleeth

Cooperating Analysis

18. W. R Wolf and J. Holden, Nutrition Institute, U.S. Department of Agriculture, Beltsville, Md

42
U. S. Department of Commerce

Malcolm Baldnge

'ficQRtvryr

National Bump of Standards

Ernest 'Ambfcr. Director

Addendum to

Rational |8uraut of jitandanfe

(Htxtiixtntt of ^nalgsts

Standard Reference Material 1568

Rice Flour

Additional Certification The following certified value is to be added to Table I.

Table I. Certified Values of Constituent Elements

3

Element Concentration, ngjg

Lead 0.045 ± 0.0l0

b

Lead should be determined on samples without drying and the results adjusted to a "dry-weight" basis by determining moisture on separate samples. The estimated uncertainty, based on judgment, is for samples 2 g or more.

Analytical Methods Used and Analysts

Inorganic Analytical Research Division, National Bureau of Standards.

Isotope dilution, mass spectrometry, 1. L. Barnes and E. S. Beary; Polarography, E. J. Maienthal.

Cooperating Analyst

R. W. Dabeka, Food Directorate, Health Protection Branch, Ottawa, Canada.

43
U.S. Department of Commerce

Elliot L. Richardson,

Secretary

National Bureau of Standards

Ernest Ambler. Acting Director

This Standard Reference Material is intended for use in calibrating instrumentation and evaluating the accuracy

of analytical methods for the determination of chromium in brewers yeast and other biological materials. SRM 1569 and like materials contain a volatile chromium component which presents an especially difficult analytical problem. Care should be taken to avoid its loss; see, "Preparation of Biological Materials for Chromium Analysis," W. R. Wolf and F. E. Greene [1],

•Calculated on a dry weight basis from determinations made on samples without drying- (See "Precautions"

below.) A minimum sample size of 150 mg should be used.

The certified value is based on concordant results by independent analytical methods; the uncertainty is estimated from the imprecision of the methods and inhomogeneity of the material.

The overall direction and coordination of the technical measurements leading to this certificate were performed

under the chairmanship of L. McClendon.

The technical aspects leading to the preparation, certification and issuance of this material were coordinated through the Office of Standard Reference Materials by R. Alvarez.

[1] Wolf, W. R. and Greene, F. E., Preparation of Biological Materials for Chromium Analysis, Proceedings

of the 7th Materials Research Symposium, Accuracy in Trace Analysis; Sampling, Sample Handling and

Analysis, NBS Spec. Publ. 422, U. S. Government Printing Office, Washington, D.C. (August 1976).

Standard Reference Material 1569

Brewers Yeast

Chromium concentration: 2. 12 ± 0.05 uglg

Washington, D.C. 20234 September 7, 1976

J. Paul Cali, Chief

Office of Standard Reference Materials

(over)

44
Analyses were performed in the NBS Analytical Chemistry Division by L. McClendon (neutron activation) and

by L. Dunstan and E. Garner (isotope dilution, mass spectrometry). Cooperative analyses were also made by W. R. Wolf, Nutrition Institute, U.S. Department of Agriculture, Beltsville, Md.

The material was furnished by the Nutrition Institute, U.S. Department of Agriculture, Beltsville, Md. At NBS,

it was passed through a sieve having openings of 0.15 mm (U.S. Series 100 standard sieve) and blended.

Precautions :

( 1) The analytical determinations should be made on samples without drying. The determinations should be corrected to a dry weight basis by heating separate samples at 85 °C for 3 hr to determine the weight loss.

(2) Samples should not be dissolved in open vessels.

Material Homogeneity was determined by a neutron activation technique using 150-mg random samples from

bottled material representing different locations of the bulk material. The statistical test pattern was proposed

by J. Mandel of the NBS Institute for Materials Research.

Stability : The material should be kept in its original bottle and stored at temperatures between 10-23 °C. Exposure to moisture should be minimized by tightly capping the bottle immediately after use. Ideally, the bottle should be kept in a desiccator at the temperature indicated.
U. S. Department of Commerce

Malcolm Baldnge

"Secretary

I V-* •

Manorial B au

Ernesi 'Ambter. Uwcctor

This Standard Reference Material (SRM) is intended primarily for use in calibrating instrumentation and evaluating

the reliability of analytical methods for the determination of major, minor, and trace elements in botanical materials,

agricultural food products, and similar matrices.

Certified Values of Constituent Elements: The certified values for the constituent elements are shown in Table 1. They

are based on results obtained either by definitive methods of known accuracy or by two or more independent analytical methods. Non-certified values, which are given for information only, appear in Table 2.

Notice and Warnings to Users:

Expiration of Certification: This certification is invalid 5 years after the shipping date. Should it be invalidated before

then, purchasers will be notified by NBS.

Stability: The material should be kept in its original bottle and stored at temperatures between 10-30 °C. It should not be exposed to intense sources of radiation, ideally, the bottle should be kept tightly closed in a desiccator in the dark at the temperature indicated.

Use: The bottle should be shaken well before each use. A minimum sample of 500 mg of the dried material (see Instructions for Drying) should be used for any analytical determination to be related to the certified values of this certificate.

Statistical consultation was provided by K. Kafadar of the Statistical Engineering Division. The overall direction and coordination of the analyses leading to this certification were performed under the chairmanship of E L. Garner, Chief of the Inorganic Analytical Research Division. The technical and support aspects involved in the preparation, certification, and issuance of this Standard Reference

Material were coordinated through the Office of Standard Reference Materials by R. Alvarez.

Washington, D.C. 20234 George A. Uriano, Chief December 20, 1982 Office of Standard Reference Materials (Revision of Certificate

Standard Reference Material 1572

Citrus Leaves

dated 2-22-82) (over)

46
Table I. Certified Values of Constituent Elements Major and Minor Constituents

Content

' Element (Wt. Percent) Cn If I ) I m 3 15 + o. 10 M agnesium A Cfl -+- A A3 U. jo 2: U.IM Phosphorus 0.13 ±0.02

Potassium* 1.82 ±0.06

Sulfur 0.407 ± 0.009

Trace Constituents Element Content,' ng/g Element Content,

1 ^g, g Aluminum 92 ± 15 Manganese 23 ±2

Arsenic 3.1 ± 0.3 Mercury 0.08 ± 0.02 Barium 21 ±3 Molybdenum 0.17 ± 0.09 Cadmium 0.03 ± 0.01 Nickel 0.6 ± 0.3 Chromium 0.8 ± 0.2 Rubidium* 4.84 ± 0.06 Copper 16.5 ± 1.0 Sodium 160 ± 20

Iodine 1.84 ± 0.03 Strontium* 100 ±2

Iron 90 ± 10 Zinc 29 ±2

Lead* 13.3 ± 2.4

Based on dry weight; For drying instructions, see the section of this certificate on instructions for Drying. The uncertainties are based on judgment and represent an evaluation of the combined effects of method imprecision, possible systematic errors among methods, and material variability for samples weighing 500 mg or more. •For those elements determined by definitive methods, the uncertainties are given as 95%/ 95% statistical tolerance intervals. See The Role of Standard

Reference Materials in Measurement Systems. NBS Monograph 148, 1975 p 14.

47
Table 2. Non-certified Values for Constituent Elements NOTE: The lollowing values are not certified because they are not based on the results of either a definitive method of known accuracy or two or more independent methods. These values are included for information only. Major Constituent

Element

Nitrogen

Content,

(Wt. Percent)

(2.86)

Trace Constituents Element Content, <ng/g Element

( 0.04 )

( 8.2 )

( 0.28 )

( 0.098) (414 )

( 0.02 )

( 0.01 )

( 0.19 )

Analytical values are based on the "dry weight'* of material (See Instructions for Drying ). Not sulficiently homogeneous for certification.

Antimony

Bromine Cerium

Cesium

Chlorine Cobalt Europium Lanthanum

Samarium

Scandium

Selenium

Tellurium

2

Thallium

Tin Uranium

Content, mK/K

( 0.052)

( 0.01 )

( 0.025)

( 0.02 ) (<0-01 )

( 0.24 ) (<0.15 )

Instructions for Drying: Samples of this SRM must be dried before weighing and analysis by either of the following

procedures:

1. Drying for 2 hours in air in an oven at 85 °C.

2. Drying for 24 hours at 20 to 25 °C and at a pressure not greater than 30 Pa (0.2 mm Hg).

Additional Information on Analyses: This SRM contains siliceous material, which is an integral part of the sample. The

values in Tables I and 2 are based on analyses performed on the entire sample. Therefore, dissolution procedures should

be capable of complete dissolution of the sample but should not result in losses of volatile elements, such as arsenic and

mercury.

Source and Preparation of Material: The plant material for this SRM was collected and prepared under the direction of A. L. Kenworthy. Michigan State University. Its source was the Lake Alfred area of central Florida. The material was

air-dried, ground in a comminuting machine to pass a 425- film (No. 40) sieve, dried at 85 °C, and thoroughly mixed in a

leed blender Alter packaging the material in polyethylene-lined fiber drums, it was sterilized in situ with cobalt-60

radiation. The sterilization procedure was carried out at the U.S. Army Research and Development Command, Natick, Mass. under the direction ol A. Brynjollsson.

48
Analytical Methods Used and Analyses

Analytical Methods:

A. Atomic absorption spectrometry

B. Atomic emission spectrometry, flame

C. Atomic emission spectrometry, inductively coupled plasma

D. Ion chromatography

E. Isotope dilution thermal source mass spectrometry

F. Isotope dilution spark source mass spectrometry

G. Kjeldahl method for nitrogen H Neutron activation

I. Photon activation

J. Polarography

K. Spectrophotometry

Analysts:

Inorganic Analytical Research Division, National Bureau of Standards

1. l.L. Barnes 14. R.M. Lindstrom

2. E.S. Beary 15. G.J. Lutz

3. K.A. Brletic 16. L.A. Machlan

4. T.A. Butler 17. E.J. Maienthal

5. E.R. Deardorff 18. J.R. Moody

6. J.W. Gramlich 19. T.J. Murphy

7. R.R. Greenberg 20. P.J. Paulsen

8. S. Hanamura 21. L.J. Powell

9. E.F. Heald 22. T.C. Rains

10. W.R. Kelly 23. T.A. Rush

II. H.M. Kingston 24. P.A. Sleeth

12. W.F. Koch 25. R.L. Watters, Jr.

13. G.M. Lambert 26. R. Zeisler

Cooperating Analysts:

1. M. lhnat. Chemistry and Biology Research Institute, Agriculture Canada, Ottawa, Canada.

2. M. Gallorini, E. Orvini, and M. DiCasa, Consiglio Nazionale delle Ricerche. Centro di Radiochimica e Analisi per Attivazione presso l'lnstituto di Chimica Generate dell' Universita. Pavia, Italy.

3. L. Kosta, A. R. Byrne, and A. Prosenc, Institute "Josef Stefan," Ljubljana, Yugoslavia.

4. J. B. Jones, Jr., Department of Horticulture, University of Georgia. Athens, Georgia.

5. U. M. Cowgill, Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania.

49
U.S. Department of Commerce

Elliot L. Richardson,

Secretary

National Bureau of Standards

Ernest Ambler. Acting Director

This Standard Reference Material is intended primarily for calibrating instrumentation and evaluating the

reliability of analytical methods for the determination of major, minor, and trace elements in botanical materials and other agricultural products.

Certified Values of Constituent Elements: The certified values for the constituent elements are shown in Table 1

.

They are based on results obtained either by reference methods of known accuracy or by two or more inde- pendent, reliable analytical methods. Non-certified values, which are given for information only, appear in Table 2. All values are based on a minimum sample size of 500 mg of the material dried as indicated under

"Instructions for Drying."

Notice and Warnings to Users: Expiration of Certification: This certification will be invalid 5 years after the shipping date. Should it be invali- dated before then, purchasers will be notified by NBS.

Stability: The material should be kept in its original bottle and stored at temperatures between 10-30 °C. It should not be exposed to intense sources of radiation, including ultraviolet lamps or sunlight. Ideally, the bottle should be kept in a desiccator in the dark at the temperature indicated.

Use: The bottle should be shaken well before each use. A minimum sample of 500 mg of the dried material

(see Instructions for Drying) should be used for any analytical determination to be related to the certified values of this certificate. The overall direction and coordination of the technical measurements leading to this certificate were performed under the chairmanship of H. L. Rook. The overall coordination of the cooperative work performed by the Commission of European Communities, Joint Research Center, Ispra Establishment, Italy, was by G. Rossi of

the Chemistry Division. The technical and support aspects involved in the preparation, certification, and issuance of this Standard

Reference Material were coordinated through the Office of Standard Reference Materials by T. W. Mears and

R. Alvarez

trf

Standard Reference Material 1573

Tomato Leaves

Washington, D.C. 20234 October 18, 1976

J. Paul Cali, Chief

Office of Standard Reference Materials

(over)

i 50
Table I. Certified Values of Constituent Elements'

Major and Minor Constituents

Trace Constituents

Element

Iron Manganese

Zinc Strontium Rubidium

Copper

Element

Potassium Calcium Phosphorus

Content Mg/g

690 ±25

238 ± 7 62 ± 6

44.9 ± 0.3

16.5 ± 0.1

11 ± 1

Content

Wt. Percent

4.46 ± 0.03

3.00 ± 0.03 0.34 ± 0.02

Element

Lead Chromium

Arsenic Thorium

Uranium

Content Mg/g

6.3 ±0.3

4.5 ±0.5

0.27 ±0.05

0.17 ±0.03

0.061 ±0.003

"Analytical values are based on the "dry-weight" of material (See Instructions for Drying). The uncertainties of the values shown in Table 1 include allowances for inhomogeneity, method imprecision, and an estimate of possible biases of the analytical methods used.

Table 2. Non-certified Values for Constituent Elements' NOTE: The following values are not certified because they are not based on the results of either a reference method of known accuracy or two or more independent methods. These values are included for information

only. Major and Minor Constituents

Trace Constituents

Element

Element

Nitrogen

Magnesium

Aluminum

Content fg g

Content

Wt. Percent

(5.0)

(0.7) (0.12)

Element Boron (30) Cobalt Bromine (26) Scandium Cadmiumb

( 3) Mercury Cerium (1.6) Thallium Lanthanum (0.9) Europium

'Analytical values are based on the "dry weight" of material (See Instructions for Drying). 'Cadmium was not sufficiently homogeneous for certification.

Content Mg/g

(0.6) (0.13)

(0.1) (0.05)

(0.04)

51
Instructions for Drying: Samples of this Standard Reference Material must be dried before weighing by either of the following procedures:

1. Drying in air in an oven at 85 °C for 2 hours.

2. Lyophilization using a cold trap at or below —50 °C at a pressure not greater than 30 Pa (0.2 mm Hg) for 24 hours. NOTE: Drying either in an oven at 105 °C or in a vacuum oven at 75°C causes large losses of volatiles other than water and should not be used.

Additional Information on Analyses: This Standard Reference Material contains siliceous material, which is an

integral part of the sample. The analyses reported in Tables I and 2 were performed on the entire sample. Therefore, dissolution procedures should be capable of complete dissolution of the sample, but should not result in losses of volatile elements, such as arsenic and mercury.

Source and Preparation of Material: The plant material for this SRM was collected and prepared under the

direction of A. L. Kenworthy of Michigan State University, East Lansing, Mich. Its source was a field plot of

direct seeded tomatoes that had been established at the Horticultural Research Center of the University. For the preparation of the SRM, the terminal portions of the plants were clipped, air-dried, and ground in a comminuting machine. After grinding, the material was dried at 85 °C, thoroughly mixed in a feed blender, packaged in polyethylene-lined fiber drums, and sterilized in situ with cobalt-60 radiation. The sterilization procedure was carried out at the U.S. Army Research and Development Command, Natick, Mass. under the direction of A. Brynjolfsson. At NBS, a preliminary evaluation of the material homogeneity indicated that its improvement would be required to establish more reliable certified values. Therefore, the material was resieved and the portion that had passed a polypropylene sieve having openings of 0.25 mm (equivalent to a U.S. series 60

standard sieve) was retained for the SRM.

Homogeneity Assessment: Material homogeneity was evaluated by determining nine of the certified elements,

P, Fe, Mn, Zn, Rb, Cu, Cr, As, and U on samples of 500 mg or less taken at various locations of the freeze-dried bulk material. The other certified elements, K, Ca, Sr, Pb, and Th were determined using sample weights not exceeding one gram. The uncertainties of the concentrations given in Table 1 include these results.

Analytical Methods Used and Analysts

Analytical Methods

A. Atomic absorption spectroscopy

B. Isotope dilution mass spectrometry

C. Isotope dilution spark source mass spectrometry D. Kjeldahl method for nitrogen

E. Neutron activation

F. Nuclear track technique G. Optical emission spectroscopy H. Spectrophotometry

I. Polarography

(over)
Analysts

Analytical Chemistry Division, National Bureau of Standards

1. R. W. Burke

2. B. S. Carpenter

3. E. R. Deardorff

4. B. I. Diamondstone

5. L. J. Dunstan

6. M. S. Epstein

7. R. H. Filby

8. E. L. Garner

9. T. E. Gills

10. J. W. Gramlich

11. S. H. Harrison

12. R. M. Lindstrom

13. L. A. Machlan

14. L. T. McClendon

15. L. J. Moore

16. T. J. Murphy

17. P. J. Paulsen

18. T. C. Rains

19. H. L. Rook

Cooperating Analysts

20. Chemistry Division, Standards and Reference Substances Secretariat, Commission of European Communities, Joint Research Center, Ispra Establishment, Italy.

G. Serrini G. Renaux W. Leyendecker

E. Orthmann

R. Pietra G. Guzzi

F. Colombo

F. Girardi N. Toussaint

21. Y. Nemoto, K.. Okamoto, and K. Fuwa, Division of Chemistry and Physics, National Institute for Environ- mental Studies, Yatabe, Ibaraki, Japan.

22. L. Kosta, Institute "Josef Stefan," Ljubljana, Yugoslavia.

23. J. B. Jones, Jr. and R. Isaac, University of Georgia, Athens, Georgia.

53
U.S. Department of Commerce

Elliot L. Richardson.

Secretary

National Bureau of Standards

Ernest Ambler. Acting Director

Rational ^Bureau of Jltantiartta

Certificate of (Analysis

Standard Reference Material 1575

Pine Needles

This Standard Reference Material is intended primarily for calibrating instrumentation and evaluating the

reliability of analytical methods for the determination of major, minor, and trace elements in botanical materials and other agricultural products.

Certified Values of Constituent Elements: The certified values for the constituent elements are shown in Table 1 . They are based on results obtained either by reference methods of known accuracy or by two or more

independent, reliable analytical methods. Non-certified values, which are given for information only, appear in Table 2. All values are based on a minimum sample size of 500 mg of the material dried as indicated under "Instructions for Drying."

Notice and Warnings to Users:

Expiration of Certification: This certification will be invalid 5 years after the shipping date. Should it be invali- dated before then, purchasers will be notified by NBS.

Stability.: The material should be kept in its original bottle and stored at temperatures between 10-30 °C. It should not be exposed to intense sources of radiation, including ultraviolet lamps or sunlight. Ideally, the bottle should be kept in a desiccator in the dark at the temperature indicated.

Use: The bottle should be shaken well before each use. A minimum sample of 500 mgof the dried material (see Instructions for Drying) should be used for any analytical determination to be related to the certified values of

this certificate. The overall direction and coordination of the technical measurements leading to this certificate were per- formed under the chairmanship of H. L. Rook. The overall coordination of the cooperative work performed by

the Commission of European Communities, Joint Research Center, Ispra Establishment, Italy, was by G. Rossi

of the Chemistry Division. The technical and support aspects involved in the preparation, certification, and issuance of this Standard

Reference Material were coordinated through the Office of Standard Reference Materials by T. W. Mears and

R. Alvarez.

Washington, D.C. 20234 October 18, 1976

J. Paul Cali, Chief

Office of Standard Reference Materials

(over)

54
Table 1. Certified Values of Constituent Elements'

Major and Minor Constituents

Trace Constituents

Element Manganese

Aluminum

Iron Rubidium

Lead

Strontium

Element Calcium

Potassium Phosphorus

Content

675 ±

545 ±

200 ±

11.7 ±

10.8 ±

4.8 ±

15 30

10

0.1 0.5 0.2

Content Wt. Percent

0.41 ±0.02

0.37 ± 0.02 0.12 ± 0.02

Element Copper

Chromium

Arsenic Mercury

Thorium

Uranium

'Analytical values are based on the "dry-weight" of material (See Instructions for Drying).

Content E&LL—

3.0 ± 0.3

2.6 ± 0.2 0.21 ±0.04

0.15 ±0.05

0.037 ± 0.003 0.020 ± 0.004

The uncertainties of the values of the constituents shown in Table 1 include allowances for material inhomogeneity, method imprecision, and an estimate of possible biases of the analytical methods used.

Table 2. Non-certified Values for Constituent Elements' NOTE: The following values are not certified because they are not based on the results of either a reference method of known accuracy or two or more independent methods. These values are included for information

only. Maior Constituent

Content

Element Wt. Percent

Nitrogen (1.2)

Trace Constituents

Content Content Element Mg/g Element Mg/g Bromine (9) Lanthanum (0.2) Nickel (3.5) Cobalt (0.1) Cerium (0.4) Thallium (0.05) Cadmium6 K0.5) Scandium (0.03) Antimony (0.2) Europium (0.006)

'Analytical values are based on the "dry-weight" of material (See Instructions for Drying) 'Cadmium was not sufficiently homogeneous for certification

55
Instructions for Drying: Samples of this Standard Reference Material must be dried before weighing by either of the following procedures:

1. Drying in air in an oven at 85 °C for 2 hours.

2. Lyophilization using a cold trap at or below -50 °C at a pressure not greater than 30 Pa (0.2 mm Hg) for 24 hours. NOTE: Drying either in an oven at 105 °C or in a vacuum oven at 75°C causes large losses of volatiles other than water and should not be used.

Additional Information on Anaiyses:This Standard Reference Material contains siliceous material,which is an

integral part of the sample. The analyses reported in Tables 1 and 2 were performed on the entire sample. Therefore, dissolution procedures should be capable of complete dissolution of the sample but should not result in losses of volatile elements, such as arsenic and mercury.

Source and Preparation of Material: The plant material for this SRM was collected and prepared under the

direction of A. L. Kenworthy of Michigan State University, East Lansing, Mich. Its source was Manistee State Park, approximately 65 km north of Muskegon, Mich. For the preparation of the SRM, the material was air- dried, and ground in a comminuting machine. After grinding the material, it was dried at 85 °C, thoroughly mixed in a feed blender, packaged in polyethylene-lined fiber drums, and sterilized in situ with cobalt-60 radiation. The sterilization procedure was carried out at the U.S. Army Research and Development Command,

Natick, Mass. under the direction of A. Brynjolfsson. At NBS, preliminary evaluation of the material homogeneity indicated that its improvement would be required to establish more reliable certified values. Therefore,

the material was resieved and the portion that passed a polypropylene sieve having openings of 0.25 mm (equivalent to a U.S. series 60 standard sieve) was retained for the SRM.

Homogeneity Assessment: Material homogeneity was evaluated by determining ten ot the certified elements, P, Al, Fe, Mn, Rb, Cu, Cr, As, Hg, and U on samples of 500 mg or less taken at various locations of the freeze-dried bulk material. The other certified elements, K, Ca, Sr, Pb, and Th were determined using sample weights not exceeding one gram. The uncertainties for the concentrations given in Table 1 include these results.

Analytical Methods Used and Analysts

Analytical Methods

A. Atomic absorption spectroscopy

B. Isotope dilution mass spectrometry

C. Isotope dilution spark source mass spectrometry

D. Kjeldahl method for nitrogen

E. Neutron activation

F. Nuclear track technique G. Optical emission spectroscopy H. Spectrophotometry

I. Polarography

(over)

56
Analysts

Analytical Chemistry Division. National Bureau of Standards

1. R. W. Burke

2. B. S. Carpenter

3. E. R. Deardorff

4. B. I. Diamondstone

5. L. J. Dunstan

6. M. S. Epstein

7. R. H. Filby

8. E. L. Garner

9. T. E. Gills

1 1. S. H. Harrison

12. R. M. Lindstrom

13. L. A. Machlan

14. E. J. Maienthal

15. L. T. McClendon

16. L. J. Moore

17. T. J. Murphy

18. P. J. Paulsen

19. H. L. Rook

10. J. W. Gramlich

Cooperating Analysts

20. Chemistry Division, Standards and Reference Substances Secretariat, Commission of European Com

munities, Joint Research Center, Ispra Establishment, Italy.

F. Girardi

21. Y. Nemoto, K. Okamoto, and K.Fuwa, Division of Chemistry and Physics, National Institute for Environ

mental Studies, Yatabe, Ibaraki, Japan.

22. R. Schelenz, Federal Research Center for Nutrition, Karlsruhe, West Germany.

23. L. Kosta, Institute "Josef Stefan," Ljubljana, Yugoslavia.

24. J. B. Jones, Jr. and R. Isaac, University of Georgia, Athens, Georgia.

G. Guzzi

A. Colombo

R. Pietra N. Toussaint
U. S. Department of Commerce

Malcolm Baldngc

Secretary

Njtionjl Burwu of Sl.jnd.jrd* ErnfNl Amhkr. Director

This Standard Reference Material (SRM) is intended primarily for use in calibrating instrumentation and evaluating

the reliability of analytical methods for the determination of major, minor, and trace elements in animal tissue and other

biological matrices.

Certified Values of Constituent Elements: The certified values for the constituent elements are shown in Table 1. Certified values are based on results obtained by definitive methods of known accuracy; or alternatively, from results obtained by two or more independent analytical methods. Noncertified values are given for information only in Table 2.

Notice and Warnings to Users:

Expiration of Certification: This certification is invalid after 5 years from the date of shipping. Should it become invalid before then, purchasers will be notified by NBS.

Stability : The material should be kept in its original bottle and stored at temperatures between 10-30 °C. It should not be exposed to intense sources of radiation. The bottle should be kept tightly closed and stored in a desiccator in the dark.

Use: A minimum sample of 250 mg of the dried material (see Instructions for Drying) should be used for any analytical determination to be related to the certified values of this Certificate.

Dissolution procedures should be designed to effect complete solution, but without losses of volatile elements, such as mercury. Dissolution for these determinations should be carried out in a closed system.

Statistical consultation was provided by K..R. Eberhardt and T.R. Crichton of the Statistical Engineering Division. The overall direction and coordination of the analyses leading to this certification were under the chairmanship of

E.L. Garner, Chief of the Inorganic Analytical Research Division. The technical and support aspects involved in the preparation, certification, and issuance of this Standard Reference

Material were coordinated through the Office of Standard Reference Materials by R. Alvarez.

Gaithersburg, MD 20899 Stanley D. Rasberry, Chief February 1, 1985 Office of Standard Reference Materials (Revision of Certificates dated 3-5-82, 6-15-82) (over)

Standard Reference Material 1577a

Bovine Liver

b8
Table 1. Certified Values of Constituent Elements

Element

Chlorine Phosphorus

Potassium* Sodium

Sulfur

Content," (Wt. Percent)

0.28 ±0.01

1.11 ±0.04

0.996 ± 0.007

0.243 ± 0.013

0.78 ±0.01

Element

Arsenic Cadmium

Calcium

Cobalt Copper

Iron Lead* Magnesium

Manganese

120

Content,

(Mg/g)

0.047 ± 0.006

0.44 ± 0.06 ± 7

0.2! ± 0.05 158

194

± 7 ±20

0.135 ± 0.015 600 ±15

9.9 ± 0.8

Element Mercury Molybdenum

Rubidium*

Selenium

Silver Strontium* Uranium* Vanadium*

Zinc

Dry weight: For drying instructions, sec the section of this Certificate on Instructions for Drying.

Content,

(Mg/g) ±0.002 ±0.5

±0.1 ±0.07

±0.01 ±0.003

0.00071 ±0.00003

0.099 ± 0.008 123 ±8

0.004

3.5

12.5

0.71 0.04

0.138

The estimated uncertainties are based on judgment and represent an evaluation of the combined effects of method

imprecision, possible systematic errors among methods, and material variability for samples weighing 250 mg or more. •For those elements determined by definitive methods, the uncertainties are given as 95%/95% statistical tolerance

limits. See "The Role of Standard Reference Materials in Measurement Systems," NBS Monograph 148, 1975 p 14.

Table 2. Noncertified Values of Constituent Elements

Content, Element

Nitrogen

(Wt. Percent)

(10.7)

Element Aluminum

Antimony

Bromine

Thallium

Content, Mg/g

(2) (0.003)

(9) (0.003) Dry weight: For drying instructions, see the section of this Certificate on instructions for Drying.
Instructions for Drying: Samples of this SRM must be dried before weighing according to the following procedure: Dry

for 24 hours at 20 to 25 °C in a vacuum oven at a pressure not greater than 30 Pa (0.2 mm Hg).

Source and Preparation of Material: The liver for this standard was obtained in the Portland, Oregon, area. The gross fat, major blood vessels, and "skin** were removed and the liver was ground. The ground liver was then mixed, transferred to polyethylene-lined trays, and

lyophilized by Oregon Freeze Dry Foods, Inc., Albany, Oregon. After lyophilization, the liver was powdered in a Tornado mill, packaged in moisture-proof bags, and then transported to the National Bureau of Standards.

Analysts and Analytical Methods Used

Analytical Methods:

A. Atomic absorption spectrometry

B. Atomic emission spectrometry, flame

C. Atomic emission spectrometry, inductively coupled plasma

D. Ion chromatography

E. Isotope dilution thermal source mass spectrometry

F. Isotope dilution spark source mass spectrometry G. Kjeldahl method for nitrogen H. Neutron activation

I. Polarography

J. Spectrophotometry

Analysts:

Analytical Chemistry Division, National Bureau of Standards:

1. J.V. Bailey 15. W.R. Kelly

2. I.L. Barnes 16. H.M. Kingston

3. E.S. Beary 17. W.F. Koch

4. CO. Blundell 18. G.M. Lambert

5. K.A. Brletic 19. R.M. Lindstrom

6. T.A. Butler 20. G.J. Lutz

7. E.R. Deardorff 21. L.A. Machlan

8. M.S. Epstein 22. E.J. Maienthal

9. J.D. Fassett 23. T.J. Murphy

10. J.W. Gramlich 24. P.J. Paulsen

II. R.R. Greenberg 25. L.J. Powell

12. S. Hanamura 26. T.C. Rains

13. S.H. Harrison 27. T.A. Rush

14. E.F. Heald 28. R.L. Watters, Jr.

29. R. Zeisler

Cooperating Analysts:

30. M. Gallorini and E. Orvini, Consiglio Nazionale delle Ricerche, Centro di Radiochimica e Analisi per Attivazione

presso P Instituto di Chimica Generate deU'Universita, Pavia, Italy.

31. L. Kosta, A.R. Byrne, M. Dermelj, Institute "Josef Stefan", Ljubljana, Yugoslavia.

60


Technical Publications

Periodical

Journal of Research—The Journal of Research of the National Bureau of Standards reports NBS research and development in those disciplines of the physical and engineering sciences in which the Bureau is active. These include physics, chemistry, engineering, mathematics, and computer sciences. Papers cover a broad

range of subjects, with major emphasis on measurement methodology and the basic technology underlying

standardization. Also included from time to time are survey articles on topics closely related to the Bureau's

technical and scientific programs. Issued six times a year.

Nonperiodicals

Monographs—Major contributions to the technical literature on various subjects related to the Bureau's scientific and technical activities. Handbooks—Recommended codes of engineering and industrial practice (including safety codes) developed in cooperation with interested industries, professional organizations, and regulatory bodies.

Special Publications—Include proceedings of conferences sponsored by NBS, NBS annual reports, and other

special publications appropriate to this grouping such as wall charts, pocket cards, and bibliographies.

Applied Mathematics Series—Mathematical tables, manuals, and studies of special interest to physicists,

engineers, chemists, biologists, mathematicians, computer programmers, and others engaged in scientific and

technical work.

National Standard Reference Data Series—Provides quantitative data on the physical and chemical properties

of materials, compiled from the world's literature and critically evaluated. Developed under a worldwide pro- gram coordinated by NBS under the authority of the National Standard Data Act (Public Law 90-396).

NOTE: The Journal of Physical and Chemical Reference Data (JPCRD) is published quarterly for NBS by

the American Chemical Society (ACS) and the American Institute of Physics (AIP). Subscriptions, reprints,

and supplements are available from ACS, 1155 Sixteenth St., NW, Washington, DC 20056.

Building Science Series—Disseminates technical information developed at the Bureau on building materials,

components, systems, and whole structures. The series presents research results, test methods, and perfor- mance criteria related to the structural and environmental functions and the durability and safety

characteristics of building elements and systems.

Technical Notes—Studies or reports which are complete in themselves but restrictive in their treatment of a

subject. Analogous to monographs but not so comprehensive in scope or definitive in treatment of the subject

area. Often serve as a vehicle for final reports of work performed at NBS under the sponsorship of other government agencies.

Voluntary Product Standards—Developed under procedures published by the Department of Commerce in Part 10, Title 15, of the Code of Federal Regulations. The standards establish nationally recognized re- quirements for products, and provide all concerned interests with a basis for common understanding of the

characteristics of the products. NBS administers this program as a supplement to the activities of the private

sector standardizing organizations.

Consumer Information Series—Practical information, based on NBS research and experience, covering areas of interest to the consumer. Easily understandable language and illustrations provide useful background

knowledge for shopping in today's technological marketplace.

Order the above NBS publications from: Superintendent of Documents, Government Printing Office,

Washington, DC 20402. Order the following NBS publications—FIPS and NBSIR 's—from the National Technical Information Service, Springfield, VA 22161.

Federal Information Processing Standards Publications (FIPS PUB)—Publications in this series collectively constitute the Federal Information Processing Standards Register. The Register serves as the official source of

information in the Federal Government regarding standards issued by NBS pursuant to the Federal Property

and Administrative Services Act of 1949 as amended, Public Law 89-306 (79 Stat. 1127), and as implemented

by Executive Order 1 1717 (38 FR 12315, dated May 11, 1973) and Part 6 of Title 15 CFR (Code of Federal

Regulations).

NBS Interagency Reports (NBSIR)—A special series of interim or final reports on work performed by NBS

for outside sponsors (both government and non-government). In general, initial distribution is handled by the sponsor; public distribution is by the National Technical Information Service, Springfield, VA 22161, in paper copy or microfiche form.
U.S. Department of Commerce

National Bureau of Standards

Gaithersburg, MD 20899

Official Business

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