# Patent

## Title
DNA encoding a fusion receptor for oncostatin M and leukemia inhibitory

## Abstract

The plasmid is useful as a vector for breeding various microorganisms such as lactic acid bacteria, and the derivatives thereof are useful also as a shuttle vector (lactic acid bacteria--Escherichia coli).

## Background
The present invention relates to a novel circular double-stranded DNA plasmid pBUL1 derived from Lactobacillus delbrueckii subsp. bulgaricus, the utility and safety of which as a yogurt-producing bacterium are well known, and to derivatives thereof, as well as to microorganisms transformed with the plasmids.

Lactic acid bacteria are very useful microorganisms which have long been used in production of various fermented foods. If a recombinant DNA technology which has rapidly been developed in recent years could be applied to lactic acid bacteria, greater enhancement of the utility of the bacteria would be expected. In fact, host-vector systems with a fairly high efficiency have already been reported for lactic acid bacteria of some species of, for example, Lactococcus lactis (reference 1), Streptococcus salivarius subsp. thermophilus (reference 2), Lactobacillus plantarum (reference 3) and Lactobacillus casei (reference 4), and the current stage of the technical improvement is on an industrial scale use of host-vector systems on an industrial scale. However, despite of great efforts by many researchers, there has hitherto been no report of transformation of Lactobacillus delbrueckii subsp. bulgaricus and Lactobacillus delbrueckii subsp. lactis (hereinafter referred to as Lb. bulgaricus, and Lb. lactis, respectively), which are used extensively as bacteria for producing milk products such as yogurt. Application of broad-host-range plasmids, such as pNZ12 (reference 5), pGK12 (reference 6) and pIL253 (reference 7), with which transformants of lactic acid bacteria of some species have been obtained, to the above-mentioned two subspecies has been attempted, but transformation was not successful. However, establishment of host-vector systems of the abovementioned two subspecies has been earnestly desired. If a recombinant DNA technology is applied to microorganisms to be used in production of foods, the safety of vectors to be used for transformation of them must be established. As vectors for this purpose, those vectors are desirable which exist naturally in microorganisms that have been used to produce foods which have traditional been and the safety of which has been historically confirmed. On the other hand, fermented milk products such as yogurt are foods which have been eaten for a long period of time and their safety has been well confirmed. Therefore, the plasmids derived from microorganisms from fermented milk products, for example, those of the abovementioned two subspecies, are useful vectors in order to construct transformants of microorganims for food production. In addition they are also available vectors for transformation for the purpose of producing various physiologically active substances.

For the purpose of developing host-vector systems for Lb. bulgaricus and Lb. lactis, the present inventors variously investigated and studied plasmids of these subspecies. As a result, they are the first who have succeeded in isolating a plasmid from Lb. bulgaricus M-878 strain (FERM BP-3758) possessed by Meiji Institute of Health Science of Meiji Milk Products Co., Ltd., which has a length of about 7 kbp, which has a restriction endonuclease map as shown in FIG. 1, and which does not have recognition sites for BamHI, EcoRI, KpnI, PstI and SalI, the base sequence (SEQ ID No. 1) of the SmaI fragment with 1344 bp being represented as the Sequence Number 1 of the Sequence Table in Table 1 below. They named the plasmid pBUL1. __________________________________________________________________________ Sequence Table: Sequence Number: 1 Length of Sequence: 1344 Type of Sequence: Nucleic Acid Number of Strand: Double-stranded Topology: Linear Kind of Sequence: Other Nucleic Acid Origin: Name of Microorganism: Lactobacillus delbrueckii subsp. bulgaricus Name of Strain: M-878 Sequence: CCCGGGGCGA AACGACATGG GGCGCTCAAA CCATTGCTGA GGCGATCAAT TACGTGCAAG 60 CCCAGCATCC CGATCACGGC TATTTCCCAG CTCGCCAAAA TTCCGGCATG AGGGTTGTTG 120 AACCGGGTGA AAGAGCCACA GGCGAAACGC TTAGAATTAC GATTGACGGA CAGGAAAGAG 180 AATTTCCGTT CAACGGCTTT TTCTATAACC GGGATTATGA AATGACCGAG GTTGGGTTTG 240 CTAACAGGTT TGCCGATTGG TACGCCAAAG GGAAACTTGT TTATCACCCC GGCTTAAAGG 300 CGTGGCTTAT GTACAACCCA GAAACGGGGT CATGGATGCC GAATGAAGAC GACAGACTGG 360 GCAAGGATTT TAACCAGACC CCGGAAAAAC TCATCGATAA CTTGCGGATA AACCTCAAAT 420 TTGAACAACC GCTATGGAAA AAAGTTGGGT TTAATCCCCA AAAGCCTAAT AATCAAACAT 480 TCGGGGAAAA GGCTTATGCT AGTGGCTATA GCCGGATCAG CACGGCCGCT GGGCAAAAGG 540 CGACCCTTGA ATTAGCTCAG AGCCGTTTAA CCGTGCGTGC ATTTAACGAC TGTAAGACCG 600 AGCTTAACAC CCAGACAGGT TGGATTGACC TCAAAACGGG TGCTATTAGC CCTCACAACC 660 CGGCGAAACT TTTTGACAAG GTCACGGATG CTGGTTTGCC TAATAAAGCC ACAGAGGGTG 720 ATGGCGGCAA GCTCTGGGAT CGTTTCCTGA AAGAGACCTT TTGCGGCGAT CTTGATTTGA 780 TCGAGTTCGT ACAGGCCTGC ATAGGCTACA GCATTACGGG CAAAATCAAT GAACAGGTCA 840 TGTTTATCTG CAAAGGCAGT GGGGGCAACG GGAAGAGCAT TTTTCTTGAA TGCTTAAACG 900 AGGTGCTGGG CGATTATAGC TCTGTTATCC CAATAGAAAC GCTAACAGAC AACGGCAAGG 960 CTCAGCGTGA CGGATCAGCA CCAAGTCCAG ACCTTGCAAG CCTTGAGGGC AAGCGGTTCG 1020 TTATTACGAG CGAACCGAAA GAGCAGGTTA CAATCGATGC TGGGACGGTC AAAACGGTGA 1080 CGGGTGGCAC TAAGTTAAAA GTTAGAATGC TACACCAAAA CCCGATTGAG TTCCTGCCAC 1140 AGTTTAAAAT TTGGTGGCAA TCTAACGGCT TGCCAAACGT CAACTTTAAC GATTATGCTA 1200 TTTTACGGCG CTTGATCGTC ATCCCGTTTA AAAATGAGGT GCOAGAGGAT GCGGTAGATA 1260 TCAACCTCAA AAGCAAGCTA ATGAAAGAGA AAGAGTTTAT TTTAAAGTGG TGTGTTGAGG 1320 GCGTGGCTAA ATGGCAAGCC CGGG                                1344 __________________________________________________________________________

FIG. 1 this is a restriction endonuclease map of pBUL1. The recognition site of each restriction enzyme is expressed by kbp unit, on the basis of BglII. pBUL1 does not have recognition sites for BamHI, EcoRI, KpnI, PstI and SalI. In the structure of pBUL1, the region necessary for replication of pBUL1 is shown by the thick line and the region corresponding to the base sequence of (SEQ ID No:1) the Sequence Number 1 (the third largest fragment of all SmaI fragments) is designated as "1344 bp SmaI fragment" in the outer periphery of the map.

FIG. 2 this shows a scheme of construction of the erythromycin-resistance cassette plasmid p8Eml, in which .DELTA. indicates the pUCll8-derived multiple cloning site.

FIG. 3 this shows restriction endonuclease maps of the derivatives of pBUL1, i. e., pX3, pX4, pS3 and pS4  (Although all of these plasmids are circular, they are represented as linear profiles on the basis of BglII.). In the drawing, the fine lines each indicate the sequence of pBUL1, and the thick lines each indicate the pAM.beta.1-derived erythromycin-resistance gene.

FIG. 4 this shows restriction endonuclease maps of plasmids pU8ST8 and pU8ST9. In the drawing, the striped arrows each indicate the L-lactate dehydrogenase gene of Streptococcus salivarius subsp. thermophilus M-192 strain (ST-LDH gene) and the fine lines each indicate the sequence of plasmid pUC118. bla indicates the ampicillin-resistance gene.

FIG. 5 this shows restriction endonuclease maps of plasmids pXL38, pXL39, pXL48 and pXL49 (Although all of these plasmids are circular, they are represented as linear profiles on the basis of BglII.). In the drawing, the fine lines each indicate the sequence of pBUL1, the thick lines each indicate the pAM.beta.1-derived erythromycin-resistance gene, and the striped arrows each indicate ST-LDH gene.

FIG. 6 this shows a scheme to locate the region required for replication of pBUL1 by the deletion method.

FIG. 7 this shows a restriction endonuclease map of pBR3.DELTA.18E1 plasmid by ligation of pX3.DELTA.18E plasmid with the E. coli plasmid (pBR322). In the drawing, the fine line indicates the sequence derived from plasmid pBR322, the thick line indicates the sequence derived from pX3.DELTA.18E, the arrow (Em.sup.r) indicates the erythromycin-resistance gene, and the arrow (Ap.sup.r) indicates the ampicillin-resistance gene.

FIG. 8 this shows a restriction endonuclease map of pBR.DELTA.18E2 plasmid. The symbols are the same as those shown in FIG. 7

FIG. 9 this shows a restriction endonuclease map of p8X3.DELTA.18E1 plasmid by ligation of pX3.DELTA.18E plasmid with the E. coli plasmid (pUC118S). In the drawing, the fine line indicates the sequence derived from plasmid pUC118S, the thick line indicates the sequence derived from pX3.DELTA.18E, the arrow (Em.sup.r) indicates the erythromycin-resistance gene, and the arrow (Ap.sup.r) indicates the ampicillin-resistance gene.

FIG. 10 this shows a restriction endonuclease map of p8X3.DELTA.18E2 plasmid. The symbols are the same as those shown in FIG. 9.

Since the phenotype encoded by the pBUL1 was cryptic, an erythromycin-resistance gene was added to the plasmid as a selective marker in transformation experiments (Erythromycin may be referred to simply as "Em" hereunder.). As a result, the present inventors have succeeded in obtaining transformants expressing the above-mentioned selective marker in microorganisms of three genera, namely Bacillus subtilis, Lactococcus lactis subsp. lactis and Lactobacillus delbrueckii subsp. lactis. The fact indicates that the plasmid pBUL1 (hereinafter often referred to as "the plasmid of the invention") has a broad host range in Gram-positive bacteria the present invention has been completed on the basis of these findings.

Since the gene which participates in self-replicatability of pBUL1 is considered to be encoded in a part of the plasmid DNA of the present invention as reported in other plasmids, any other plasmid derivatives derived either by deletion of unnecessary regions of replication from the plasmid of the invention, or by insertion or addition of any other DNA to pBUL1 are also considered to have the same function as the plasmid of the invention. For instance, as described in Example 6 followed hereinafter, any plasmid causes no hindrance in replication, if it contains, as a region necessary for replication, a region necessary for replication in about 4 kbp DNA fragment indicated by the thick line in FIG. 1 between the recognition site for Eco47III and the position about 1.1 kbp apart clockwise from the ScaI site, namely about 0.45 kbp apart counterclockwise from the NdeI site. Therefore, the present invention is not restricted to only the plasmid pBUL1 itself but includes other derivative plasmids as obtained by modifying pBUL1 as well as other recombinant plasmids as obtained by inserting other gene(s), for example, marker(s) such as Em-resistance gene or exogeneous gene(s) such as L-lactate dehydrogenase gene, or other promoter(s) or operator(s) into it.

As derivative plasmids of pBUL1 of the present invention, for example, there are mentioned plasmids containing necessary regions for replication in the region of about 4 kbp shown as the thick line in FIG. 1 as mentioned above. As one example of them, there is the mentioned plasmid pX3.DELTA.18E (FIG. 6, the bottom). This plasmid can be replicated not only in Gram-positive bacteria such as lactic acid bacteria but also in E. coli, as is obvious from Example 8 . This plasmid itself is nothing but a plasmid with a broad-hostrange, which has been desired in this technical field. Since this plasmid is one which can be replicated not only in Gram-positive bacteria (e.g., lactic acid bacteria, Bacillus subtilis) but also in Gram-negative bacteria (e.g., E. coli), it is effective as a novel versatile shuttle vector which has extremely high applicability and can be used in both Gram-positive bacteria and Gram-negative bacteria. Another examples of the pBULl-derived plasmids can be obta by ligation of other plasmids (fragments) with the region of pBUL1 necessary for replication, involved in the region of about 4 kbp shown as the thick line area in FIG. 1, or a DNA fragment of pBUL1 which contains this region. As the other plasmids to be ligated, various plasmids may be used widely. As some examples of them, there are exemplified E. coli-derived plasmids such as pBR series plasmids and pUC series plasmids.

Also the thus ligated plasmids have been identified to be replicable in both lactic acid bacteria and E. coli, like the above-mentioned plasmid pX3.DELTA.18E, and they may also be used as plasmids with a broad-host-range which have heretofore been strongly desired in this technical field or as shuttle vectors between Gram-positive bacteria and Gram-negative bacteria. As examples of such ligated plasmids, there are exemplified pBR3.DELTA.18E1, pBR3.DELTA.18E2, p8X3.DELTA.18E1, p8X3.DELTA.18E2 (FIGS. 7 to 10), etc.

As mentioned above, plasmid pBUL1 and its derivative plasmids show a broad-host-range and are useful. Using them as a vector, expression of heterogeneous genes in lactic acid bacteria is possible.

For instance, where a recombinant plasmid was constructed by ligation of pBUL1 plasmid with an erythromycin-resistance gene as a selective marker gene and an L-lactate dehydrogenase gene derived from a yogurt lactococcus (Streptococcus salivaris su) (e.g., pXL38 in FIG. ) as an exogenous gene, and the recombinant plasmid was introduced into Lb. lactis which does not naturally have L-lactic acid producibility, the resulting erythromycin-resisrance transformant produced not only D-lactic acid but also almost the same amount of L-lactic acid as metabolic end-products.

Thus, plasmid pBUL1 and its derivative plasmids are useful also as a vector for expression of heterogeneous genes in lactic acid bacteria.

For preparing the plasmid pBUL1 of the present invention, L. bulgaricus M-878 strain is first cultivated in a liquid medium which is used for cultivation of lactic acid bacteria, for example, LCM medium (reference 8), according to ordinary methods of cultivating lactic acid bacteria under ordinary incubation conditions. Next, the incubated cells are collected and are then subjected to lysis by known methods for lactic acid bacteria, for example, by using an enzyme such as lysozyme or mutanolysin, etc. From the resulting cell lysate, the intended plasmid can be isolated and purified by a conventional method, such as phenol extraction and cesium chloride density gradient centrifugation in the presence of ethidium bromide. For construction of the derivative plasmids of pBUL1 which are other plasmids of the present invention, the plasmid pBUL1 may be treated for digestion, ligation and others by known methods (reference 10).

In order to transform microorganisms by introducing thereinto a recombinant plasmid as obtained by inserting a selective marker gene to the plasmid pBUL1, a known method which is considered to be the best for the host may be selected from conventional methods, such as calcium chloride method, protoplast-polyethylene glycol method, electroporation method, etc., with no particular limitation, according to the characteristics of microorganisms to be transformed. Where lactic acid bacteria are used as a host, the electroporation method is preferred. A marker for selecting the transformants may be selected from various antibiotic-resistance genes known in this technical field. Where the transformed microorganisms are intended to be used in producing foods or medicines, use of markers which have been confirmed to be safe is desirable. If desired, other base sequences participating in control of expression, such as promoters, may also be inserted into the plasmids of the invention.

Recombinant plasmids are considered to be safe which are constructed from pBUL1 plasmid or its derivative, an enzyme gene derived from a microorganism used in food production and a safe selective marker gene. Therefore, the transformants of food-producing bacteria such as lactic acid bacteria with such a safe plasmid are also considered to be safe. Such safe transformants may be incubated by ordinary methods to produce a large amount of enzymes and physiologically active substance and they may be used in various food productions in ordinary ways to attain the intended objects. In addition, since the safety of the transformants is highly assured in any case, they do not cause by-production of any biohazards or harmful substances. Accordingly, it is expected that they could advantageously be utilized in industrial production of medicines and foods which especially need safe methods of production. Next the present invention will be explained hereunder by way of the following examples.

Lactobacillus delbrueckii subsp. bulgaricus M-878 strain (FERM BP-3758, as possessed by Meiji Institute of Health Sciences herein often referred to simply as M-878 strain) was subcultivated with a skim milk medium (liquid medium as prepared by dissolving 10% skim milk powder and 0.1% yeast extract in distilled water and sterilized at 121.degree. C. for 7 minutes) and inoculated (0.5%) in 6 liters of LCMG medium (as prepared by adding 1% (w/v) glucose to LC medium) and incubated at 37.degree. C. for 15 hours.

After the incubation, the cells were collected by centrifugation and washed twice with 20 m Tris-HCl buffer (pH 7.0). The washed cells were suspended in 480 ml of a hypertonic buffer (20 mM Tris buffer containing 0.3M raffinose, 5 mM magnesium chloride and 5 mM calcium chloride; pH 7.0). To this were added mutanolysin and lysozyme in an amount of 5 .mu.g/ml and 500 .mu.g/ml, respectively, as the final concentrations, and were incubated at 37.degree. C. for 10 minutes. Then, 54 ml of 250 mM EDTA (pH 8.0) was added to the resulting solution, which was then subjected to centrifugation to collect the precipitates.

The precipitates were then suspended in 240 ml of 50 mM Tris buffer (pH 8.0) containing 6.7% (w/v) sucrose and 25 mM EDTA. The resulting suspension was then processed according to Anderson & Mckay method (reference 9), from lysis with SDS to rough purification of plasmid DNA.

The crude plasmid DNA preparation thus obtained was subjected to RNase treatment by an ordinary method (reference 10) and then to cesium chloride density gradient centrifugation in the presence of ethidium bromide to obtain about 1 .mu.g of purified pBUL1 plasmid DNA.

The pBUL1 plasmid was cut with various commercially available restriction enzymes, and the length of each fragment obtained was calculated after agarose gel electrophoresis. As a result, pBUL1 was identified to be a circular double-stranded DNA plasmid having the restriction endonuclease map in FIG. 1 with a total length of about 7.9 kbp. pBUL1 did not have recognition sites for BamHI, EcoRI, KpnI, PstI and SalI. Of five fragments obtained by digestion of pBUL1 with SmaI, the third largest fragment (1344 bp; the position of which has been designated in the outer periphery of the restriction endonuclease map in FIG. 1 as "1344 bp SmaI fragment") was. analyzed with respect to the base sequence thereof (SEG ID No: 1), which is shown as Sequence Number 1 in Table 1.

First, conjugatively transmissible plasmid pAM.beta.1  (reference 11) derived from Enterococcus faecalis was cut with HhaI and subjected to agarose gel electrophoresis to thereby cut out a gel fraction containing DNA fragments ranging about 1.1 kbp having the Em-resistance gene. DNAs were isolated from the thus cut-out gel using a GENECLEAN DNA purifying kit (product by BIO101 Co.). The DNA fragment was ligated to E. coli-derived pUC118 plasmid (product by Takara Shuzo Co.) according to the process shown in FIG. 2 to prepare "cassette plasmid"p8Eml in order to excise the Em-resistance gene successfully with various restriction enzymes. Next, about 0.25 .mu.g of p8Eml DNA was cut with XbaI and was ligated with a fragment as obtained by cutting about 0.025 .mu.g of pBUL1 DNA with XbaI. A half of the reaction mixture after the ligation was used in transformation of Bacillus subtilis 207-25 strain (reference 13) by the method of Chang et al (reference 12). One third of the transformed cells were spread on a plate of DM3 medium containing 25 .mu. g/ml of erythromycin and incubated for 2 days at 37.degree. C. to obtain Em-resistant transformants.

Plasmid DNAs were prepared from the transformants obtained, and the restriction endonuclease cleavage pattern of the plasmids was analyzed. When pBUL1 and the Em-resistance gene were ligated at XbaI site, five transformants of all the nine analyzed contained a plasmid having the restriction endonuclease map of A in FIG. 3 and two of them contained a plasmid having the restriction endonuclease map of B in FIG. 3. The plasmid with the map A in FIG. 3 was named pX3 and that with the map B in the same was named pX4.

In the same manner as above, except that p8Eml was cut with SmaI and pBUL1 was cut with ScaI, and the resulting fragments were subjected to blunt end ligation followed by transformation of Bacillus subtilis 207-25 strain with the ligated products, Em-resistant transformants were also obtained. Five of the six transformants, analyzed with respect to plasmids therein, contained a plasmid having the restriction endonuclease map of C in FIG. 3 and one of them contained a plasmid having the restriction endonuclease map of D in FIG. 3. These plasmids were named pS3 and pS4, respectively. As mentioned above, recombinant plasmids pX3, pX4, pS3 and pS4 (all having a length of about 9.0 kbp) were constructed by introducing the pAM.beta.1-derived Em-resistance gene (having a length of about 1.1 kb) to pBUL1. In addition, it was shown that pBUL1 could function as a plasmid replicon in Bacillus subtilis.

Lactococcus lactis subsp. lactis (hereinafter often referred to simply as "Lc. lactis") IL1403 strain (as obtained from Dr. Alain Chopin, INRA, France) was used. To this was introduced pBUL1 having the Em-resistance gene, whereby a transformant of Lc. lactis showing Em-resistance was successfully obtained. The details are mentioned below.

From the transformants of Bacillus subtilis obtained in Example 2, the recombinant plasmids, PX3 and pX4, constructed by inserting the Em-resistance gene into pBUL1 were prepared by the method of reference 14. Next, pX3 and pX4 each were introduced into Lc. lactis IL1403 strain according to the method of reference 1. The transformants were obtained by selecting on agar plates of BL medium (product by Eiken Chemical Co.) containing 25 .mu.g/ml of Em.

The transformants were incubated in LCMG medium, and plasmids were obtained from the cells after Anderson et al. (reference 9). The plasmids thus obtained were shown to have the same restriction enzyme recognizing sites as those of the plasmids used for the transformation. From the result, it was concluded that pBUL1 also functioned as a plasmid replicon in not only Bacillus subtilis but also Lc. lactis.

From the transformants of Lactococcus lactis subsp. lactis IL1403 obtained in Example 3, plasmids pX3 and pX were prepared. Using them, Lb. lactis ATCC 12315 strain and M-908 strain, possessed by Meiji Institute of Health Science, were transformed by electropotation. There was no report of success in transformation of Lactobacillus delbrueckii species being commercially useful despite studies by many researchers. Using the plasmid of the invention, transformation of Lb. lactis was attained for the first time, as mentioned below.

Lb. lactis ATCC 12315 strain or M-908 strain as subcultivated in a skim milk medium was inoculated in LCMG medium in a concentration of 2% and incubated for 2 hours at 42.degree. C. The cells were collected and washed, and then suspended in EP buffer (containing 0.4M sucrose, 1 mM magnesium chloride and 7 mM potassium dihydrogenphosphate; pH 7.4) at a concentration of OD.sub.660 =4.0 and cooled on ice. 0.8 ml of the cell suspension was put in an electroporation cuvette, about 0.1 to 2 .mu.g of pX3 or pX4 plasmid was added thereto, and an electric pulse of 25 .mu.F at 2.5 kV was discharged thereto with Gene Pulser (manufactured by Bio-Rad Co.).

Immediately after the discharge, the cells were suspended in 4 ml of an expression medium (LCMG medium to which 0.2M raffinose, 5 mM magnesium chloride and 1% lactose had been added) and incubated statically for 2.5 hours at 37.degree. C. All the culture liquid thus incubated was poured into several plates and 10 to 15 ml of BL agar medium (sterilized and kept at 50.degree.C.) containing 25 .mu.g/ml of Em was added thereto and mixed. After the culture medium solidified, the plates were incubated anaerobically in Gaspak system (manufactured by Beckton-Dickinson Co.) at 37.degree. C. for 2 to 4 days, and the transformants were selected. According to the method, about 10 to 100 transformants per .mu.g of the plasmid DNA were obtained.

The Em-resistant clones thus obtained showed a strong Em-resistance (>1 mg Em/ml) and had a plasmid having the same restriction endonuclease map as pX3 or pX4. From these results, they were confirmed to be transformants. Further, using the pX3 or pX4 plasmid DNA obtained from the transformants, the transformation frequency in Lb. lactis ATCC 12315 strain increased by about 10 times.

No transformants of Lb. lactis have heretofore been obtained by using other plasmids such as pGK12 or IL253 under the same conditions as above. From the above, it is obvious that the pBUL1 of the present invention is useful as a vector for Lb. lactis. Dehydrogenase Gene inserted in pBUL1)

A restriction enzyme SspI fragment (about 1.2 kbp) containing a gene (Japanese Patent Application No. 2-45976) coding for L-lactate dehydrogenase (hereinafter referred to as "ST-LDH") derived from a lactic acid bacterium Streptococcus salivarius subsp. thermophilus M-192 strain (possessed by Meiji Institute of Health Science) was inserted into the SmaI recognition site of pUCll8, to construct the recombinant plasmids pU8ST8 and pU8ST9 as shown in FIG. 4-A and FIG. 4-B, respectively. These recombinant plasmids were cut with BamHI and KpnI and subjected to agarose gel electrophoresis. About 0.02 .mu.g of DNA fragments containing the ST-LDH gene was cut out and isolated using a GENECLEAN DNA purification kit (manufactured by BIO101 Co. ). These DNA fragments were ligated with about 0.3 .mu.g of pX3 or pX4, obtained in Example 2, cut with BamHI and KpnI. Using this ligation mixture, Lc. lactis IL1403 strain was transformed, whereby transformants having the plasmids as shown in FIG. 5 were obtained. These plasmids were named pXL38, pXL39, pXL48 and pXL49, respectively.

These plasmids were prepared by the method of reference 9 and were used for transformation of Lactobacillus delbrueckii subsp. lactis ATCC 12315 strain by the same method as shown in Example 4. As a result, Em-resistant transformants were obtained. These carried plasmids each having the same restriction endonuclease map as the introduced plasmids.

These transformants were incubated in a skim milk medium. The culture was diluted with distilled water and subjected to centrifugation. The lactic acid in the resulting supernatant was measured by the use of a lactic acid measuring kit (F Kit L-lactic Acid; manufactured by Boehringer Mannheim). As a result, L-lactic acid, which is not naturally produced at all by the host, was detected in an amount almost equivalent to D-lactic acid produced.

The cells of one of the transformants were disrupted and the cell extract was prepared. As a result, L-lactate dehydrogenase activity, which is not detected in the host cells, was detected in the cell extract of the transformant. The L-lactate dehydrogenase from the transformant was purified and the sequence of the 18 N-terminal amino acids thereof was examined. As a result, it was identical to that of ST-LDH. From the above-mentioned results, it was shown that a heterogeneous gene expression was possible in Lb. lactis with pBUL1 as a replicon. a Deletion Method)

pX3 was cut with BamHI and KpnI and subjected to deletion in the direction as indicated in FIG. 6, by the use of a DNA deletion kit for kilosequencing (manufactured by Takara Shuzo Co.). Each reaction mixture was applied to transform Lc. lactis IL1403 in the same method as in Example 3, and the transformants were selected in the presence of 25 .mu.g/ml of Em.

Plasmids harboured by the Em-resistant transformants were prepared and their restriction enzyme recognition sites were examined. As a result, the shortest deletant of pX3 was obtained whose deletion proceeded to the position in pX3 shown in FIG. 6, namely, the position about 1.1 kbp clockwise apart from ScaI site and about 0.4 kbp counterclockwise apart from NdeI site. The deleted plasmid was named pX3.DELTA.18 and it was further cut with both PstI and either of BglII, SphI and Eco47III and then subjected to self-circularizing ligation. The reaction mixture was used in transformation of Lc. lactis IL1403 strain and the selection of the transformants was performed as in Example 3. As a result, transformants having plasmids of various sizes were obtained. The plasmid pX3.DELTA.18E as shown in FIG. 6 is the shortest one, which was formed by cutting pX3.DELTA.18 with PstI and Eco47III followed by self-ligation. The region necessary for replication of pBUL1 was found to be contained in about 4 kbp DNA fragment of pX3.DELTA.18E. Plasmid with E. coli Plasmid)

pX3.DELTA.18E plasmid obtained in Example 6 was prepared from Lc. lactis IL1403 strain by the method of reference 9. The thus obtained pX3.DELTA.18E was cut with SphI in the multi-cloning site and was ligated with a DNA fragment obtained by cutting E. coli plasmid pBR322 (manufactured by Takara Shuzo Co.) or pUC118 with SphI. The reaction mixture was used to transform E. coli TG1 strain [.DELTA.(lac-pro)supE thi hsdD/F' traD36  proA+B+lacI.sup.q lacZ.DELTA.M15](manufactured by Amersham Co.) by the well-known calcium chloride method. The transformants were selected with 500 .mu.g Em/ml. They had a recombinant plasmid composed of pX3.mu.18E and pBR322 or pUC118, as shown in FIG. 7 to FIG. 10 (pBR3.mu.18E1, pBR3.mu.18E2; p8X3.mu.18E1, p8X 3.mu.18E 2).

These transformants showed ampicillin resistance (50 .mu.g/ml) derived from E. coli pBR322 and pUC118 plasmids. These recombinant plasmids isolated and purified from E. coli were used to transform Lc. lactis IL1403 strain and transformants were selected with 25 .mu.g/ml of Em as shown in Example 3. As a result, Em-resistant transformants were obtained. The plasmids possessed by the transformants were prepared and the restriction enzyme-recognition sites were examined. As a result, they each had the same restriction endonuclease map as the map of those prepared from E. coli. From these results, pX3.DELTA.18E, one of the derivatives of pBUL1, was found to be a useful shuttle vector if it is ligated with an E. coli plasmid. For the transformants with such a recombinant plasmid could be selected with ampicillin and Em, or Em when the host was either E. coli or lactic acid, respectively. Thus, the usefulness of pBUL1 was further proved.

The plasmid pX3.DELTA.18E, obtained in Example 6, was prepared from the transformant of Lc. lactis IL1403 by the method of reference 9, and was used to transform E. coli TG1 strain in the same manner as in Example 7. The thus obtained Em-resistant (Em 500 .mu.g/ml) transformant had a plasmid having the same restriction endonuclease map as that of pX3.DELTA.18E used for the transformation. From the fact, it was found that the pX3.DELTA.18E plasmid, a derivative of pBUL1, is replicable even in E. coli, that E. coli with pX3.DELTA.18E may be selected on the basis of the Em resistance and that pX3.DELTA.18E is useful as a shuttle vector between Gram-positive bacteria and Gram-negative bacteria. Thus, the usefulness of pBUL1 was further proved.

References: 3119-3123. 2130-2137. 173-177. 549-552. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. Kiso Kohza (Lectures on Fundamental Microbiology) (Ando, T. and Sakaguchi, K. (eds.)), pp. 168-215 (1987)

Advantage of the Invention:

The species Lactobacillus delbrueckii is an industrially useful lactic acid bacterium, including subsp. bulgaricus and subsp. lactis which are important in production of yogurt and cheese and subsp. delbrueckii which is important in production of lactic acid. However, with respect to Lactobacillus delbrueckii, neither transformation nor the presence of an autonomously replicable plasmid has heretofore been reported. The plasmid of the present invention is the first reported up to now. Using the plasmid of the present invention as a replicon, transformation of Lb. lactis which is one of this species, has become possible for the first time. Accordingly, it is expected that various genes are inserted into the plasmid of the present invention to give recombinant plasmids which are introduced into subspecies of Lactobacillus delbrueckii to construct improved strains having useful characteristics, for example, a high lactose metablizing ability or an improved milk protein degrading activity. In addition, the present invention provides a plasmid with a broad-host-range shuttle vector. Thus, the effectiveness of the present invention is further elevated.

Since the plasmid of the invention is one isolated from a strain of Lb. bulgaricus existing in yogurt, its safety has been confirmed historically. In addition, since the plasmid of the invention can replicate not only in species of Lactobacillus delbrueckii but also in other bacteria important in the food industry, such as the genera Bacillus and Lactococcus, it is expected to be useful not only in the production of various foods but also in breeding of micro-organisms which produce various enzymes and physiologically active substances.

Reference to Deposited Microorganism under Rule 13-2:

1. Lactobacillus delbrueckii subsp. bulgaricus M-878 Microorganism: Technology, Ministry of International Trade and Industry SEQUENCE LISTING (1) GENERAL INFORMATION: (iii) NUMBER OF SEQUENCES: 1 (2) INFORMATION FOR SEQ ID NO:1: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1344 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: double (D) TOPOLOGY: linear (ii) MOLECULE TYPE: DNA (vi) ORIGINAL SOURCE: (A ) ORGANISM: Lactobacillus delbrueckii (B) STRAIN: M-878 (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1: CCCGGGGCGAAACGACATGGGGCGCTCAAACCATTGCTGAGGCGATCAATTACGTGCAAG60 CCCAGCATCCCGATCACGGCTATTTCCCAGCTCGCCAAAATTCCGGCATGAGGGTTGTTG120 AACCGGGTGA AAGAGCCACAGGCGAAACGCTTAGAATTACGATTGACGGACAGGAAAGAG180 AATTTCCGTTCAACGGCTTTTTCTATAACCGGGATTATGAAATGACCGAGGTTGGGTTTG240 CTAACAGGTTTGCCGATTGGTACGCCAAAGGGAAACTTGTTTATCACCCCGGCTT AAAGG300 CGTGGCTTATGTACAACCCAGAAACGGGGTCATGGATGCCGAATGAAGACGACAGACTGG360 GCAAGGATTTTAACCAGACCCCGGAAAAACTCATCGATAACTTGCGGATAAACCTCAAAT420 TTGAACAACCGCTATGGAAAAAAGTTGGGTTT AATCCCCAAAAGCCTAATAATCAAACAT480 TCGGGGAAAAGGCTTATGCTAGTGGCTATAGCCGGATCAGCACGGCCGCTGGGCAAAAGG540 CGACCCTTGAATTAGCTCAGAGCCGTTTAACCGTGCGTGCATTTAACGACTGTAAGACCG600 AGCTTAACAC CCAGACAGGTTGGATTGACCTCAAAACGGGTGCTATTAGCCCTCACAACC660 CGGCGAAACTTTTTGACAAGGTCACGGATGCTGGTTTGCCTAATAAAGCCACAGAGGGTG720 ATGGCGGCAAGCTCTGGGATCGTTTCCTGAAAGAGACCTTTTGCGGCGATCTTGA TTTGA780 TCGAGTTCGTACAGGCCTGCATAGGCTACAGCATTACGGGCAAAATCAATGAACAGGTCA840 TGTTTATCTGCAAAGGCAGTGGGGGCAACGGGAAGAGCATTTTTCTTGAATGCTTAAACG900 AGGTGCTGGGCGATTATAGCTCTGTTATCCCA ATAGAAACGCTAACAGACAACGGCAAGG960 CTCAGCGTGACGGATCAGCACCAAGTCCAGACCTTGCAAGCCTTGAGGGCAAGCGGTTCG1020 TTATTACGAGCGAACCGAAAGAGCAGGTTACAATCGATGCTGGGACGGTCAAAACGGTGA1080 CGGGTGGCAC TAAGTTAAAAGTTAGAATGCTACACCAAAACCCGATTGAGTTCCTGCCAC1140 AGTTTAAAATTTGGTGGCAATCTAACGGCTTGCCAAACGTCAACTTTAACGATTATGCTA1200 TTTTACGGCGCTTGATCGTCATCCCGTTTAAAAATGAGGTGCGAGAGGATGCGGT AGATA1260 TCAACCTCAAAAGCAAGCTAATGAAAGAGAAAGAGTTTATTTTAAAGTGGTGTGTTGAGG1320 GCGTGGCTAAATGGCAAGCCCGGG1344

## Claims

1. Isolated plasmid pBUL1.

2. The DNA fragment designated by the thick line in FIG. 1 which region is about 4 kbp between the position of the Eco47III site and the position about 1.1 kbp clockwise from the ScaI site and about 0.45 kbp counterclockwise from the NdeI site, which region is necessary for replication of pBUL1.

3. A plasmid into which the DNA fragment of claim 2 has been inserted.

4. A microorganism transformed with the plasmid of claim 3, wherein the microorganism is Lactococcus lactis.

5. A microorganism transformed with the plasmid of claim 3, wherein the microorganism is Bacillus subtilis.