# Patent

## Title
Protein-liposome conjugates

## Abstract

This is a continuation-in-part of our copending application Ser. No. 611,669, filed on May 18, 1984 now abandoned.

## Background
Interleukin 1 (IL-1) is a protein produced by activated mononuclear phagocytes and performs a broad range of functions in host defense and immunoregulation (Dinarello, C. A. [1984] New England J. Med. 311, 1413-1418). Recently it has been demonstrated that Il-1 is first synthesized as a precursor molecule of about 270 amino acids in length (approximately 30,000 molecular weight) which is proteolytically processed into a smaller molecule (approximately 18,000 molecular weight) which possesses full biological activity (Auron, P. E., Webb, A. C., Rosenwasser, L. J., Mucci, S. F., Rich, A., Wolff, S. M., and Dinarello, C. A. [1984] Proc. Natl. Acad. Sci. USA 81). The sequence for human IL-1 is shown in Chart A.

The subject invention concerns truncated human IL-1 cDNA sequences which encode biologically-active human IL-1 proteins. These truncated cDNA sequences, and novel biologically-active human IL-1 proteins obtained therefrom, can be obtained by genetic engineering procedures using a clone containing the entire human IL-1 cDNA sequence as starting material. Specifically, with reference to Chart A, the nucleotide sequence located between residues 534 and 893 encode biologically-active IL-1 proteins. Within this range are two regions which encode biologically-active IL-1 proteins; i.e., (1) the nucleotide sequence located between residues 534 and 710, and (2) the nucleotide sequence located between residues 711 and 893.

The subject invention, advantageously, provides novel biologically-active human IL-1 proteins through use of novel truncated human IL-1 cDNA sequences. As disclosed above, the entire human IL-1 cDNA sequence is shown in Chart A. This sequence is the starting material for the preparation of the novel clones of the subject invention, as disclosed hereinafter in the Examples.

Clone (plasmid)pcD-415, which contains the cDNA for human monocyte IL-1, was deposited in an E. coli HB101 host in the permanent collection of the Northern Regional Research Laboratory, U.S. Department of Agriculture, Peoria, Ill., USA, on Apr. 27, 1984. The culture was assigned the accession number NRRL B-15770 by the repository. This deposit is available to the public upon the grant of a patent disclosing it. The deposit is also available as required by foreign patent laws in countries when counterparts of the subject application, or its progeny, are filed. However, it should be understood that the availability of a deposit does not constitute a license to practice the subject invention in derogation of patent rights granted by governmental action.

Recombinant plasmid pcD-415 can be isolated from its E. coli HB101 host by well-known procedures, e.g., using cleared lysate-isopycnic density gradient procedures, and the like.

Unlimited amounts of nucleic acid comprising nucleotide sequences coding for truncated human IL-1 can be made by the cloned human IL-1 cDNA of the subject invention. Further, the IL-1 proteins produced by the cloned cDNA of the subject invention can be used to induce the production of IL-2 by activating T-cells-IL-2 stimulates the T-cells to proliferate. As reported in Science, 221, 1362-1364, "Researchers from NIAID and the Food and Drug Administration (FDA), using a test tube assay, have recently found that interleukin-2 improved the function of T-cells from six AIDS patients" (p. 1362). In summary, the novel biologically-active human IL-1 proteins obtained via the cloned truncated human IL-1 cDNA sequences of the subject invention can be used in the same manner as native human IL-1.

The following examples are illustrative of the process and products of the subject invention but are not to be construed as limiting. All percentages are by weight and all solvent mixture proportions are by volume unless otherwise noted. for Proteins Corresponding to the DNA Sequences Located Between Nucleotide Positions 87 Through 677, and Positions 1355 Through 1396 Shown in Chart A

The IL-1 cDNA sequence (Chart A) contains three unique restriction endonuclease digestion sites that can be used to construct plasmids containing specific deletions aimed at isolating essential domains of IL-1. Proceeding 5' to 3' in the directional sense of protein coding by the cDNA, these three sites are located respectively named and positioned as follows: Hind III (pos. 483); Pvu II (pos. 678); and Xmn I (pos. 1355) (Note: all restriction endonuclease sites presented here are referenced to the location of the first nucleotide on the 3' side of scission as read along the protein coding "sense" strand of the cDNA). In addition a unique Pst I restriction site located upstream from the cDNA sequence (pos. -16) can also be used.

The first plasmid construction deletes all IL-1 cDNA nucleotide sequence between the Pvu II and Xmn I sites, described above, and is as follows: Plasmid pL1, as described by H. Okayama and P. Berg (1983) Molec. Cell. Biol. 3:280-289, and which can be purchased from Pharmacia (Piscataway, NJ), is digested completely with Xmn I and Hind III restriction endonucleases. Three products which result can be resolved by agarose gel electrophoresis.

These products are approximately 518, 782, and 1544 base pairs in length. The 518 base pair fragment is isolated from the agarose gel using standard techniques. Another plasmid, e.g., pUC-8 (Messing, J. and Vieira, J. [1982] Gene 19:269-276), which can be purchased from Pharmacia, is used as source of a DNA fragment which can be used as a linker segment to attach the Pst I restriction site located at one end of the 518 base pair fragment to a Hind III site which will be described below. pUC-8 contains a polycloning site with adjacent Pst I and Hind III sites and can be substituted for by other similar DNAs such as pUC-9 or M13mp8 or M13mp9 double stranded replicative forms. These DNAs can be purchased from Pharmacia. The pUC-8 plasmid is digested with Pst I and mixed with the 518 base pair fragment derived from pL1. The two fragments are ligated by T4 DNA ligase under conditions of excess pUC-8. Two products which result represent two different ligated orientations of the 518 fragment with respect to the linearized pUC-8. The two different orientations cannot easily be isolated from each other since each possesses the same molecular size (approximately 3660 base pairs). Isolation is accomplished by first digesting the 3660 base pair DNA mixture with Hind III endonuclease which causes the original mixture to be fragmented into 4 products of approximately 3650, 3140, 528, and 10 base pairs in length. These products can readily be resolved by standard agarose gel electrophoresis and the 528 base pair, pL1-derived, fragment (which now possess Hind III cohesive ends) is isolated.

The original human IL-1 cDNA plasmid (pcD-415), contained in the E. coli HB101 host, is isolated using standard plasmid preparation procedures. This plasmid is digested with both Pvu II and Xmn I restriction endonucleases to yield three products which are resolvable by agarose gel electrophoresis (approximate sizes are 675, 1633, and 2379 base pairs). The 1633 and 2379 base pair fragments are isolated from the gel and ligated in the presence of T4 DNA ligase to the pL1-derived, 528 base-pair fragment, described above. Two different plasmid constructs result, one of which has the proper orientation for the DNA fragments. The correct construct can readily be isolated by taking advantage of the fact that the ampicillin resistance gene contained within the pcD-415 plasmid will be properly reassembled only in the plasmid construction containing the desired IL-1 cDNA fragment orientation. Therefore E. coli HB101 cells transformed with the mixture containing both plasmids will only yield viable E. coli cells containing the proper construct when the cells are grown in the presence of ampicillin. From these cells the final construct (which is referred to as pcD-415.DELTA.Pvu/Xmn) can be isolated using standard plasmid isolation procedures. This plasmid contains truncated human IL-1 cDNA that codes for a protein corresponding to the DNA sequence located between nucleotide positions 87 through 677 and positions 1355 through 1396 shown in Chart A. for a Protein Corresponding to the DNA Sequence Located Between Nucleotide Positions 492 Through 893 Shown in Chart A

This plasmid is constructed such that all the cDNA sequence between the upstream Pst I site and the Hind III site contained within the human IL-1 sequence is deleted. The starting material is plasmid pcD-415. Plasmid pcD-415 is digested with Hind III endonuclease and the two products (approximately 1016 and 3676 base pairs) resolved by agarose gel electrophoresis. The 3676 base pair fragment is isolated from the gel and mixed with the pL1-derived, 528 base pair (Hind III cohesive-ended) fragment prepared for use in constructing pcD-415.DELTA.Pvu/Xmn in Example 1. Ligation of these DNAs by T4 ligase results in two different plasmid products which can be purified and distinguished by transformation of E. coli HB101 cells and restriction mapping of the isolated plasmids. A Pvu II and Pst I double digestion permits clear identification of the product. The final product with the required deletion is referred to as pcD-415.DELTA.Pst/Hin. This plasmid contains a truncated human IL-1 cDNA that codes for a protein corresponding to the DNA sequence located between nucleotide positions 492 through 893 shown in Chart A. for Proteins Corresponding to the DNA Sequence Located Between Nucleotide Positions 492 Through 677 and Positions 1355 Through 1396 Shown in Chart A

This construction is a combination of both deletions described above located within a single plasmid. The pcD-415.DELTA.Pst/Hin plasmid, described above, is digested with Pvu II and Xmn I to yield three agarose gel resolvable products (approximately 675, 1150, and 2379 base pairs). The 1150 and 2379 base pair fragments are isolated and ligated to yield two possible products which can be resolved in a fashion analogous to that described in Example 1 by selection of transformed E. coli HB101 in the presence of ampicillin. The final product with the required deletions is referred to as pcD-415.DELTA.Pst/Hin-.DELTA.Pvu/Xho.

This plasmid contains a truncated human IL-1 cDNA that codes for proteins corresponding to the DNA sequence located between nucleotide positions 492 through 677 and positions 1355 through 1396 shown in Chart A.

The cDNA transcript can be obtained from the clones in essentially pure form by standard art methods. For example, the cDNA transcript can be clipped from a plasmid by a BamHI-Pst I double-digestion (Okayama, H. and Berg, P. [1983] Molec. Cell. Biol. 3:280-289) and isolated by standard procedures. The essentially pure cDNA thus obtained can be used for subcloning into a different transfer vector.

As is well known in the art, the amino acid sequence of a protein, e.g., the IL-1 proteins of the invention, is determined by the nucleotide sequence of the DNA. Because of the redundancy of the genetic code, i.e., more than one coding nucleotide triplet (codon) can be used for most of the amino acids used to make proteins, different nucleotide sequences can code for a particular amino acid. Thus, the genetic code can be depicted as follows: Phenylalanine (Phe) TTK      Histidine (His) CAK Leucine (Leu) XTY      Glutamine (Gln) CAJ Isoleucine (Ile) ATH      Asparagine (Asn) AAK Methionine (Met) ATG      Lysine (Lys)   AAJ Valine (Val) GTL      Aspartic acid (Asp) GAK Serine (Ser) QRS      Glutamic acid (Glu) GAJ Proline (Pro) CCL      Cysteine (Cys) TGK Threonine (Thr) ACL      Tryptophan (Try) TGG Alanine (Ala) GCL      Arginine (Arg) WGZ Tyrosine (Tyr) TAK      Glycine (Gly)  GGL Termination Signal TAJ Termination signal TGA ______________________________________

Key: Each 3-letter deoxynucleotide triplet corresponds to a trinucleotide of mRNA, having a 5'-end on the left and a 3'-end on the right. All DNA sequences given herein are those of the strand whose sequence corresponds to the mRNA sequence, with thymine substituted for uracil. The letters stand for the purine or pyrimidine bases forming the deoxynucleotide sequence.

The above shows that the novel amino acid sequences of the human IL-1 proteins of the subject invention can be prepared by nucleotide sequences other than those disclosed herein. Functionally equivalent nucleotide sequences encoding the novel amino acid sequences of these human IL-1 proteins, or fragments thereof having IL-1 activity, can be prepared by known synthetic procedures. Accordingly, the subject invention includes such functionally equivalent nucleotide sequences.

Thus the scope of the subject invention includes not only the specific nucleotide sequences depicted herein, but also all equivalent nucleotide sequences coding for molecules with substantially the same human IL-1 biological activity. The term "equivalent" is being used in its ordinary patent usage here as denoting a nucleotide sequence which performs substantially as the nucleotide sequence identified herein to produce molecules with substantially the same human IL-1 biological activity in essentially the same kind of hosts. Within this definition are subfragments which have human IL-1 biological activity.

It is well within the skill of those in the genetic engineering art to use the nucleotide sequences encoding human IL-1 activity of the subject invention to produce human IL-1 proteins via microbial processes. Fusing the sequences into an expression vector and transforming or transfecting into hosts, either eukaryotic (yeast or mammalian cells) or prokaryotic (bacterial cells), are standard procedures used in producing other well-known proteins, e.g., insulin, interferons, human growth hormone, and the like. Similar procedures, or obvious modifications thereof, can be employed to prepare human IL-1 proteins by microbial means or mammalian tissue-culture technology in accord with the subject invention.

The nucleotide sequences obtained from IL-1 clone pCD-415 also can be prepared by a "gene machine" by procedures well known in the art. This is possible because of the disclosure of the nucleotide sequence. However, it is generally recognized in the art at this time that obtention of the desired nucleotide sequence from a clone, e.g., pCD-415, is the most expedient way to practice an invention such as disclosed and claimed herein.

The restriction enzymes disclosed can be purchased from Bethesda Research Laboratories, Gaithersburg, MD, or New England Biolabs, Beverly, MA. The enzymes are used according to the instructions provided by the supplier.

The various methods employed in the preparation of the plasmids and transformation of host organisms are well known in the art. These procedures are all described in Maniatis, T., Fritsch, E. F., and Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, New York. Thus, it is within the skill of those in the genetic engineering art to extract DNA from microbial cells, perform restriction enzyme digestions, electrophorese DNA fragments, tail and anneal plasmid and insert DNA, ligate DNA, transform cells, e.g., E. coli cells, prepare plasmid DNA, electrophorese proteins, and sequence DNA. ##STR1##

## Claims

1. A protein having the following amino acid sequence:

2. A protein having the following amino acid sequence: ##STR2##

3. A protein having the following amino acid sequence: ##STR3##