# Patent

## Title
Vaccine carrier

## Abstract

The present invention relates to a hypoallergenic protein consisting of at least one hypoallergenic molecule derived from an allergen, which is fused or conjugated to at least one second non-allergenic protein or fragment thereof.

## Background
The present invention relates to novel hypoallergenic molecules and uses thereof.
Type I allergy is an IgE-mediated hypersensitivity disease affecting almost 25% of the population. It is based on the recognition of harmless airborne, insect, venom, food allergen and contact allergen antigens derived from per se harmless antigen sources such as pollen, insects, mold and animal proteins by specific immunoglobulin E. The crosslinking of effector cell-bound IgE antibodies leads to a release of inflammatory mediators (e.g., histamine, leukotrienes) and thus to the immediate symptoms of allergy (e.g., rhinoconjunctivitis, asthma, dermatitis, anaphylaxis). T-cell activation via IgE-dependent as well as IgE-independent mechanisms contributes to chronic allergic inflammation.
The probably only causative forms of allergy treatment is allergen-specific immunotherapy, which is based on the repeated administration of increasing amounts of allergen extracts for most sources. Numerous clinical studies have documented the clinical efficacy of injection immunotherapy and there is evidence for several immunological mechanisms underlying this treatment. Due to the difficulty to prepare high quality allergen extracts for certain allergen sources and the fact that the administration of allergens to patients can cause severe side effects, allergen-specific immunotherapy can only be recommended for certain patients groups and disease manifestations. It is especially difficult to treat patients with co-sensitizations to several different allergen sources and patients suffering from severe disease manifestations such as allergic asthma. Allergic asthma is one of the most vigorous manifestations of allergy, because it severely affects the quality of daily life, causes a high rate of hospitality admissions and can manifest itself in serious, life-threatening forms requiring intensive care of the patient.
Allergen extracts prepared from natural allergen-sources are crude in nature, and it is impossible to influence the quality and amounts of individual allergens in such preparations by technical means. They also contain numerous undefined non-allergenic components, and several recent studies indicate the poor quality of such extracts and document their great heterogeneity.
In the last decade great progress has been made in the field of molecular allergen characterization using recombinant DNA technology. A large number of the most important disease-eliciting allergens has been characterized down to the molecular level, and recombinant allergens mimicking the epitope complexity of natural allergen extracts have been produced. Moreover, several research groups have used the knowledge regarding allergen structures to develop defined new allergy vaccines. Genetic engineering, synthetic peptide chemistry and conjugation of allergens with immunostimulatory DNA sequences have been used to reduce the allergenic activity of the new vaccines and thus the rate of therapy-induced side effects. First promising clinical studies were conducted with such allergen derivatives. Interestingly, it turned out that although IgE-reactivity of genetically engineered recombinant allergens and allergen-derived synthetic T-cell epitope-containing peptides could be strongly reduced or even abolished, these derivatives still could induce systemic side effects appearing several hours after injection. For example, it was reported that T-cell epitope peptides of the major cat allergen, Fel d 1, induced asthma and bronchial hyper reactivity several hours after intracutaneous injection, and there is strong evidence that this effect is T-cell mediated and MHC-restricted.
These results indicate that the removal of IgE-reactivity diminishes IgE-mediated side effects since no immediate reactions were recorded in the course of these immunotherapy studies. However, the allergen-specific T-cell epitopes which have been preserved in the recombinant allergen derivatives as well as in the peptide mixtures are responsible for the late side effects (e.g. very problematic or atopic dermatitis, chronic T cell-mediated allergic skin manifestation). The side effects caused in the case of recombinant allergen-derivatives were relatively mild and in the case of the T-cell peptide vaccines may be overcome by adequate dosing. Both of the two new approaches therefore seem very promising for immunotherapy of allergic rhinoconjunctivitis but may have limitations when it comes to the treatment of severe forms of allergic asthma, where the induction of late side effects in the lung may be very problematic.
In order to administer and consequently to provoke an efficient immune response against peptides, polypeptides and proteins, adjuvants and/or carriers are regularly used. Complete Freund's adjuvant, for instance, is one of the most potent adjuvants available. However, because of its side effects, its use is not approved for humans. Therefore, there exists a need for vaccine compositions able to induce strong immune responses against peptides and polypeptides derived from allergens and of course of other antigens avoiding the use of complete Freund's adjuvant. Further, while BSA has been used successfully as a carrier in animal models it may not be appropriate for use in human vaccine compositions because of the risk of adverse reactions such as the risk of transmitting prion disease (variant Creutzfeldt-Jakob disease). A further challenge to the development of an effective vaccine against allergens is the need for an immune response able to rapidly decrease allergens in an individual or animal. Therefore, high concentrations of allergen-specific antibodies in the blood, which are mainly of the IgG subtype, are needed. In mucosal surfaces IgA antibodies are the primary subtype.
Cholera toxin, a known carrier protein in the art, is also used regularly as an adjuvant, eliminating the need for complete Freund's adjuvant in a vaccine composition. However, cholera toxin increases total and specific IgE antibody levels and leads to IgE-associated inflammatory reactions.
Due to the side effects provoked by most carrier proteins used for vaccination, there exists a need for carrier systems which are able to stimulate immune responses against allergens or other antigens, without using toxic adjuvants, without using poorly tolerated carrier proteins and, in certain situations, without stimulation of potentially pathologic immune responses. Novel carrier systems meeting these specifications can be used towards the formation of novel conjugates and compositions suitable for the treatment or prevention of diseases like allergic diseases.
In Bohle B. et al. (J. Immunol. 172 (11) (2004): 6642-6648) a recombinant fusion protein comprising an S-layer protein moiety and Bet v 1 moiety is described. This molecule comprises the native hyperallergenic Bet v 1 protein.
WO 2004/004761 relates to virus like particles which are fused to an immunogen and which may be used for immunisation.
In WO 2004/003143 the use of fusion proteins comprising a virus like particle and a hyperallergenic molecule as immunogen for vaccination is disclosed.
It is an object of the present invention to provide medicaments and carriers which overcome the aforementioned drawbacks and allow an allergen vaccination with reduced side effects.
Therefore, the present invention relates to a hypoallergenic protein consisting of at least one hypoallergenic molecule derived from an allergen, which is fused or conjugated to at least one second non-allergenic protein or fragment thereof.
In order to provoke an enhanced immune response against a molecule, in particular of a hypoallergenic molecule according to the present invention, said molecule is fused (by genetic engineering) or conjugated (by chemical reactions) to a carrier. A conventional and regularly employed carrier is, for instance, KLH (Keyhole limpet hemocyanin). KLH, which is isolated from the giant sea mollusc Megathura crenulata, is one of the most popular carrier proteins used to create an immunogen for injection. KLH induces a strong antibody response because of its large mass and because it is a non-mammalian protein.
The second protein (the “carrier” or “carrier protein”) to be fused or conjugated to a hypoallergenic molecule of the invention is not derived from an allergen (“non-allergenic”). However, the carrier protein used in the present invention may exhibit T cell reactivity and/or provoke an immune response against itself and the hypoallergenic molecule fused or conjugated to it when administered to an animal or human body. Consequently, if the carrier protein is derived from a pathogen (e.g. virus, bacteria etc.), (protecting) antibodies directed to said carrier and pathogens are produced.
As used herein, “hypoallergenic protein” means a fusion protein/polypeptide of a carrier of a non-allergenic source with a hypoallergenic molecule. Furthermore, a “hypoallergenic protein” is also intended to be a conjugation product (e.g. chemical coupling, adsorption) of a carrier with a hypoallergenic molecule.
“Hypoallergenic” as used herein, refers to molecules with reduced allergenic potential. Such molecules have a decreased capacity to provoke allergic reactions in an individual compared to the wild-type protein from which these molecules are derived.
The at least one hypoallergenic molecule derived from an allergen and fused/conjugated to a second protein is preferably C- and/or N-terminally truncated. “C- and/or N-terminal truncation”, as used herein, means that amino acid residues either from the N-terminus or from the C-terminus or both from the N- and C-terminus of the wild-type allergen are removed by deletion of at least 1, 2, 3, 4, 5, 7, 10, 15, 20, 30 amino acid residues.
The hypoallergenic molecules, i.e. peptides/polypeptides, comprise preferably 10 to 50 amino acids, more preferably 15 to 40 amino acids, in particular 20-30 amino acids and exhibit reduced IgE reactivity. These molecules are designed to exclude T-cell epitopes which may cause T-cell-mediated side effects. T-cell epitopes and molecules exhibiting reduced T-cell response may be determined and identified by methods known by the person skilled in the art (e.g., Bercovici N. et al. Clin Diagn Lab Immunol. (2000) 7:859-864).
It was found that it is possible to design peptide vaccines derived from allergens like the major grass pollen allergens, e.g., Phl p 1, and for the major birch pollen allergen, Bet v 1, using surface exposed peptides. The data obtained show that such peptide vaccines can be produced for any allergen whose primary structure is known according to IgE epitope mapping, three-dimensional structure data or computer-aided prediction of surface-exposed domains. However, the selection of suitable peptides which may be used for vaccination remains crucial, because not all peptides identified with these methods can be employed in vaccination. The peptides suitably used for vaccination purposes should exhibit reduced IgE-binding capacity and—in order to reduce or avoid late side effects—exhibit reduced T-cell reactivity.
The term “derived from an allergen”, as used herein, means that the hypoallergenic molecules according to the present invention are obtained directly from an allergen by fragmentation or truncation. The amino acid sequence of the hypoallergenic molecules of the present invention are preferably at least 80% identical, more preferably at least 90% identical, most preferably at least 95% identical, in particular 100% identical, to the amino sequence stretch of the wild-type allergen, from which the hypoallergenic molecule is derived. However, the molecules which are not 100% identical to the wild-type allergen fragments should be able to bind with at least 60%, preferably at least 70%, more preferably at least 80%, most preferably at least 90%, strength to an antibody or to antibodies, preferably to IgG antibodies, which are directed to said wild-type allergen fragments.
The degree of identity of a first amino acid sequence to a second amino acid can be determined by a direct comparison between both amino acid sequences using certain algorithms. Such algorithms are, for instance, incorporated in various computer programs (e.g. “BLAST 2 SEQUENCES (blastp)” (Tatusova et al. (1999) FEMS Microbiol. Lett. 174:247-25; Corpet F, Nucl. Acids Res. (1988) 16:10881-10890).
The truncated molecules according to the present invention can be defined as being parts of the complete allergen that induce less activation of allergen-specific T cells than the complete wild-type allergen (preferably at least a 30%, more preferably at least a 50%, most preferably at least a 70%, reduction), exhibit a more than 50% reduced (preferably more than 70%) allergenic activity as evaluated by IgE binding assays and ability to induce IgE-mediated cell activation and when coupled to a carrier as described induce IgG antibodies which inhibit the binding of polyclonal IgE from allergic patients to the complete wild-type allergen.
The peptides should contain sequences from the allergens to avoid overlaps with the mimotopes. Mimotopes, however, which are small peptide mimics (less than 15 amino acids) of antigen pieces and are obtained from random peptide libraries do not represent original, allergen-derived molecules as defined herein. They can not be used according to the invention because they are too small to induce a robust blocking IgG response.
The hypoallergenic molecules according to the present invention may be obtained by recombinant methods or chemical synthesis. Alternatively, it is, of course, also possible to obtain the molecules by enzymatic or chemical cleavage of the wild-type allergen or a polypeptide/protein harbouring the molecule of interest.
The hypoallergenic molecule may comprise preferably at least two truncated allergen molecules derived from at least one allergen, wherein the order of the truncated allergen fragments differs from the order of the fragments in the wild-type allergen if the at least two molecules are derived from the same allergen.
The hypoallergenic molecule according to the present invention may comprise one or more (preferably at least 2, more preferably at least 3) hypoallergenic molecules as defined herein, thus, resulting in a fusion protein. The single hypoallergenic molecules of the fusion protein, which, of course, also lacks IgE-binding capacity and lacks T-cell epitopes, may be derived from allergens of the same and/or of different origin. If the molecules are derived from the same allergen, the order in the hypoallergenic fusion protein should not be identical to the order in the wild-type allergen (this prevents the reconstitution and formation of IgE-binding sites) (see, e.g., WO2004/065414, Linhart B and Valenta R (Int Arch Allergy Immunol. (2004) 134:324-31)).
According to a preferred embodiment of the present invention the at least one hypoallergenic molecule is fused to the N-terminus and/or C-terminus of said at least one second protein or fragment thereof.
The allergen or fragments thereof may be conjugated chemically, e.g., or by recombinant methods to each other. If the allergen or fragment thereof is conjugated chemically to a carrier, said allergen or fragment should be provided with a terminal cysteine residue (resulting in a free sulfhydryl group). To said terminal, (N- or C-terminal) cysteine residue any maleimide-activated carrier protein may be conjugated, thus creating an immunogen/carrier complex. If the allergen or fragment thereof does not have a sulfhydryl group at a terminus, EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride) chemistry in order to couple amines (lysine) or carboxylic acids (glutamic, aspartic acid or 5-phosphate) to the carrier protein may be employed.
If the hypoallergenic molecule fused to the N- or C-terminus of the carrier, recombinant methods are employed.
According to a preferred embodiment of the present invention the at least one second protein is a viral, in particular RNA or DNA viral, bacterial fungal or protozoal protein.
The at least one second protein (“carrier”) may be of any of the above-mentioned origin. It is, however, in particular preferred to use proteins which provoke an immune response against the protein itself and the hypoallergenic molecule fused or conjugated thereto. Due to the induction of formation of (protective) antibodies directed also to the at least one second protein, the hypoallergenic protein according to the present invention may also be employed as vaccine for said second protein and its originating source (e.g. virus, bacteria, fungus). Of course it is also possible to use carrier proteins well known in the art (e.g. KLH) as the at least second protein.
The viral protein according to the present invention is preferably a capsid protein.
Viral capsid proteins are especially suited because they induce antiviral activity, provoke the formation of antibodies which block adhesion of viruses, e.g. rhinoviruses, to epithelial cells, exhibit an immuno-modulatory activity towards a Th1 response, increase the immunogenicity of the peptide (i.e., higher anti-peptide and hence higher levels of protective IgG antibodies), are suited and proved for prophylactic vaccination (virus vaccination) and are safe, when capsid proteins are used to whose humans are continuously exposed (e.g. rhinoviruses).
According to another preferred embodiment of the present invention the at least one viral capsid protein is derived from a human pathogenic virus, preferably a virus of the family of picornaviridae.
The virus of the family of picornaviridae is preferably of the genus of rhinoviruses, preferably of the species of human rhinoviruses, in particular human rhinovirus 89 and 14. The capsid protein may be VP1, VP2, VP3 and/or VP4.
The allergen to be fused to a viral capsid protein is preferably selected from the group consisting of major birch pollen allergens, in particular Bet v 1 and Bet v 4, major timothy grass pollen allergens, in particular Phl p 1, Phl p 2, Phl p 5, Phl p 6 and Phl p 7, major house dust mite allergens, in particular Der p 1 and Der p 2, major cat allergen Fel d 1, major bee allergens, major wasp allergens, profilins, especially Phl p 12, and storage mite allergens, especially Lep d 2.
Other suited allergens to be used according to the present invention can be derived from the following table.










ALLERGENS
















cDNA







(C) or




Biochem. ID or

protein
Reference,


Species Name
Allergen Name
Obsolete name
MW
(P)
Acc. No.















Ambrosia artemisiifolia
Amb a 1
antigen E
8
C
8, 20


short ragweed
Amb a 2
antigen K
38
C
8, 21



Amb a 3
Ra3
11
C
22



Amb a 5
Ra5
5
C
11, 23



Amb a 6
Ra6
10
C
24, 25



Amb a 7
Ra7
12
P
26


Ambrosia trifida
Amb t 5
Ra5G
4.4
C
9, 10, 27


giant ragweed


Artemisia vulgaris
Art v 1

27-29
C
28


mugwort
Art v 2

35
P
28A



Art v 3
lipid transfer protein
12
P
53



Art v 4
profilin
14
C
29


Helianthus annuus
Hel a 1

34

29A


sunflower
Hel a 2
profilin
15.7
C
Y15210


Mercurialis annua
Mer a 1
profilin
14-15
C
Y13271


Caryophyllales


Chenopodium album
Che a 1

17
C
AY049012, 29B


lamb's-quarters, pigweed,
Che a 2
profilin
14
C
AY082337


white goosefoot
Che a 3
polcalcin
10
C
AY082338


Salsola kali
Sal k 1

43
P
29C


Russian-thistle


Rosales


Humulus japonicus
Hum j 4w


C
AY335187


Japanese hop


Parietaria judaica
Par j 1
lipid transfer protein 1
15
C
see list of







isoallergens



Par j 2
lipid transfer protein 2

C
see list of







isoallergens



Par j 3
profilin

C
see list of isoallergens


Parietaria officinalis
Par o 1
lipid transfer protein
15

29D


B. Grasses


Poales


Cynodon dactylon
Cyn d 1

32
C
30, S83343


Bermuda grass
Cyn d 7


C
31, X91256



Cyn d 12
profilin
14
C
31a, Y08390



Cyn d 15

9
C
AF517686











Cyn d 22w
enolase
data pending













Cyn d 23
Cyn d 14
9
C
AF517685



Cyn d 24
Pathogenesis-related p.
21
P
pending


Dactylis glomerata
Dac g 1
AgDg1
32
P
32


orchard grass
Dac g 2

11
C
33, S45354



Dac g 3


C
33A, U25343



Dac g 5

31
P
34


Festuca pratensis
Fes p 4w

60
—


meadow fescue


Holcus lanatus
Hol l 1


C
Z27084


velvet grass


Lolium perenne
Lol p 1
group I
27
C
35, 36


rye grass
Lol p 2
group II
11
P
37, 37A, X73363



Lol p 3
group III
11
P
38



Lol p 5
Lol p IX, Lol p Ib
31/35
C
34, 39



Lol p 11
hom: trypsin inhibitor
16

39A


Phalaris aquatica
Pha a 1


C
40, S80654


canary grass


Phleum pratense
Phl p 1

27
C
X78813


timothy
Phl p 2


C
X75925, 41



Phl p 4


P
41A



Phl p 5
Ag25
32
C
42



Phl p 6


C
Z27082, 43



Phl p 11
trypsin inhibitor hom.
20
C
AF521563, 43A



Phl p 12
profilin

C
X77583, 44



Phl p 13
polygalacturonase
55-60
C
AJ238848


Poa pratensis
Poa p 1
group I
33
P
46


Kentucky blue grass
Poa p 5

31/34
C
34, 47


Sorghum halepense
Sor h 1


C
48


Johnson grass


C. Trees


Arecales


Phoenix dactylifera
Pho d 2
profilin
14.3
C
Asturias p.c.


date palm


Fagales


Alnus glutinosa
Aln g 1

17
C
S50892


alder


Betula verrucosa
Bet v 1

17
C
see list of isoallergens


birch
Bet v 2
profilin
15
C
M65179



Bet v 3


C
X79267



Bet v 4

8
C
X87153, S54819



Bet v 6
h: isoflavone reductase
33.5
C
see list of isoallergens



Bet v 7
cyclophilin
18
P
P81531


Carpinus betulus
Car b 1

17
C
see list of isoallergens


hornbeam


Castanea sativa
Cas s 1

22
P
52


chestnut
Cas s 5
chitinase



Cas s 8
lipid transfer protein
9.7
P
53


Corylus avellana
Cor a 1

17
C
see list of isoallergens


hazel
Cor a 2
profilin
14
C



Cor a 8
lipid transfer protein
9
C



Cor a 9
11S globulin-like protein
40/?
C
Beyer p.c.



Cor a 10
luminal binding prot.
70
C
AJ295617



Cor a 11
7S vicilin-like prot.
48
C
AF441864


Quercus alba
Que a 1

17
P
54


White oak


Lamiales


Oleaceae


Fraxinus excelsior
Fra e 1

20
P
58A, AF526295


ash


Ligustrum vulgare
Lig v 1

20
P
58A


privet


Olea europea
Ole e 1

16
C
59, 60


olive
Ole e 2
profilin
15-18
C
60A



Ole e 3

9.2

60B



Ole e 4

32
P
P80741



Ole e 5
superoxide dismutase
16
P
P80740



Ole e 6

10
C
60C, U86342



Ole e 7

?
P
60D, P81430



Ole e 8
Ca2+-binding protein
21
C
60E, AF078679



Ole e 9
beta-1,3-glucanase
46
C
AF249675



Ole e 10
glycosyl hydrolase hom.
11
C
60F, AY082335


Syringa vulgaris
Syr v 1

20
P
58A


lilac


Plantaginaceae


Plantago lanceolata
Pla 1 1

18
P
P842242


English plantain


Pinales


Cryptomeria japonica
Cry j 1

41-45
C
55, 56


sugi
Cry j 2


C
57, D29772


Cupressus arisonica
Cup a 1

43
C
A1243570


cypress


Cupressus sempervirens
Cup s 1

43
C
see list of isoallergens


common cypress
Cup s 3w

34
C
ref pending


Juniperus ashei
Jun a 1

43
P
P81294


mountain cedar
Jun a 2


C
57A, AJ404653



Jun a 3

30
P
57B, P81295


Juniperus oxycedrus
Jun o 4
hom: calmodulin
29
C
57C, AF031471


prickly juniper


Juniperus sabinoides
Jun s 1

50
P
58


mountain cedar


Juniperus virginiana
Jun v 1

43
P
P81825, 58B


eastern red cedar


Platanaceae


Platanus acerifolia
Pla a 1

18
P
P82817


London plane tree
Pla a 2

43
P
P82967



Pla a 3
lipid transfer protein
10
P
Iris p.c.


D. Mites


Acarus siro
Aca s 13
arthropod
14* 
C
AJ006774


mite

fatty acid binding prot.


Blomia tropicalis
Blo t 1
cysteine protease
39
C
AF277840


mite
Blo t 3
trypsin
24* 
C
Cheong p.c.



Blo t 4
alpha amylase
56
C
Cheong p.c.



Blo t 5


C
U59102



Blo t 6
chymotrypsin
25
C
Cheong p.c.



Blo t 10
tropomyosin
33
C
61



Blo t 11
paramyosin
110
C
AF525465, 61A



Blo t 12
Bt11a

C
U27479



Blo t 13
Bt6, fatty acid bind prot.

C
U58106



Blo t 19
anti-microbial pep. hom.
7.2
C
Cheong p.c.


Dermatophagoides farinae
Der f 1
cysteine protease
25
C
69


American house dust mite
Der f 2

14
C
70, 70A, see list of







isoallergens



Der f 3
trypsin
30
C
63



Der f 7

24-31
C
SW: Q26456, 71



Der f 10
tropomyosin

C
72



Der f 11
paramyosin
98
C
72A



Der f 14
mag3, apolipophorin

C
D17686



Der f 15
98k chitinase
98
C
AF178772



Der f 16
gelsolin/villin
53
C
71A



Der f 17
Ca binding EF protein
53
C
71A



Der f 18w
60k chitinase
60
C
Weber p.c.


Dermatophagoides microceras
Der m 1
cysteine protease
25
P
68


house dust mite


Dermatophagoides pteronyssinus
Der p 1
antigen P1, cysteine protease
25
C
62, see list of


European house dust mite




isoallergens



Der p 2

14
C
62A-C, see list of







isoallergens



Der p 3
trypsin
28/30
C
63



Der p 4
amylase
60
P
64



Der p 5

14
C
65



Der p 6
chymotrypsin
25
P
66



Der p 7

22/28
C
67



Der p 8
glutathione transferase

C
67A



Der p 9
collagenolytic serine pro.

P
67B



Der p 10
tropomyosin
36
C
Y14906



Der p 14
apolipophorin like prot.

C
Epton p.c.


Euroglyphus maynei
Eur m 2


C
see list of isoallergens


mite
Eur m 14
apolipophorin
177
C
AF149827


Glycyphagus domesticus
Gly d 2


C
72B, see isoallergen


storage mite




list


Lepidoglyphus destructor
Lep d 2 Lep d 1

15
C
73, 74, 74A, see


storage mite




isoallergen list



Lep d 5


C
75, AJ250278



Lep d 7


C
75, AJ271058



Lep d 10
tropomyosin

C
75A, AJ250096



Lep d 13


C
75, AJ250279


Tyrophagus putrescentiae
Tyr p 2


C
75B, Y12690


storage mite


E. Animals


Bos domesticus
Bos d 2
Ag3, lipocalin
20
C
76, see isoallergen


domestic cattle




list


(see also foods)
Bos d 3
Ca-binding S100 hom.
11
C
L39834



Bos d 4
alpha-lactalbumin
14.2
C
M18780



Bos d 5
beta-lactoglobulin
18.3
C
X14712



Bos d 6
serum albumin
67
C
M73993



Bos d 7
immunoglobulin
160

77



Bos d 8
caseins
20-30

77


Canis familiaris
Can f 1

25
C
78, 79


(Canis domesticus)
Can f 2

27
C
78, 79


dog
Can f 3
albumin

C
S72946



Can f 4

18
P
A59491


Equus caballus
Equ c 1
lipocalin
25
C
U70823


domestic horse
Equ c 2
lipocalin
18.5
P
79A, 79B



Equ c 3
Ag3-albumin
67
C
79C, X74045



Equ c 4

17
P
79D



Equ c 5
AgX
17
P
Goubran Botros p.c.


Felis domesticus
Fel d 1
cat-1
38
C
15


cat (saliva)
Fel d 2
albumin

C
79E, X84842



Fel d 3
cystatin
11
C
79F, AF238996



Fel d 4
lipocalin
22
C
AY497902



Fel d 5w
immunoglobulin A
400

Adedoyin p.c.



Fel d 6w
immunoglobulin M
 800-1000

Adedoyin p.c.



Fel d 7w
immunoglobulin G
150

Adedoyin p.c.


Cavia porcellus
Cav p 1
lipocalin homologue
20
P
SW: P83507, 80


guinea pig
Cav p 2

17
P
SW: P83508


Mus musculus
Mus m 1
MUP
19
C
81, 81A


mouse (urine)


Rattus norvegius
Rat n 1

17
C
82, 83


rat (urine)


F. Fungi (moulds)


1. Ascomycota


1.1 Dothideales


Alternaria alternata
Alt a 1

28
C
U82633



Alt a 2

25
C
83A, U62442



Alt a 3
heat shock prot.
70
C
U87807, U87808



Alt a 4
prot. disulfideisomerase
57
C
X84217



Alt a 6
acid ribosomal prot. P2
11
C
X78222, U87806



Alt a 7
YCP4 protein
22
C
X78225



Alt a 10
aldehyde dehydrogenase
53
C
X78227, P42041



Alt a 11
enolase
45
C
U82437



Alt a 12
acid ribosomal prot. P1
11
C
X84216


Cladosporium herbarum
Cla h 1

13

83B, 83C



Cla h 2

23

83B, 83C



Cla h 3
aldehyde dehydrogenase
53
C
X78228



Cla h 4
acid ribosomal prot. P2
11
C
X78223



Cla h 5
YCP4 protein
22
C
X78224



Cla h 6
enolase
46
C
X78226



Cla h 12
acid ribosomal prot. P1
11
C
X85180


1.2 Eurotiales


Aspergillus flavus
Asp fl 13
alkaline serine protease
34

84


Aspergillus fumigatus
Asp f 1

18
C
M83781, S39330



Asp f 2

37
C
U56938



Asp f 3
peroxisomal protein
19
C
U20722



Asp f 4

30
C
AJ001732



Asp f 5
metalloprotease
40
C
Z30424



Asp f 6
Mn superoxide dismut.
26.5
C
U53561



Asp f 7

12
C
AJ223315



Asp f 8
ribosomal prot. P2
11
C
AJ224333



Asp f 9

34
C
AJ223327



Asp f 10
aspartic protease
34
C
X85092



Asp f 11
peptidyl-prolyl isomeras
24

84A



Asp f 12
heat shock prot. P90
90
C
85



Asp f 13
alkaline serine protease
34

84B



Asp f 15

16
C
AJ002026



Asp f 16

43
C
g3643813



Asp f 17


C
AJ224865



Asp f 18
vacuolar serine protease
34

84C



Asp f 22w
enolase
46
C
AF284645



Asp f 23
L3 ribosomal protein
44
C
85A, AF464911


Aspergillus niger
Asp n 14
beta-xylosidase
105
C
AF108944



Asp n 18
vacuolar serine protease
34
C
84B



Asp n 25
3-phytase B
 66-100
C
85B, P34754



Asp n ?

85
C
Z84377


Aspergillus oryzae
Asp o 13
alkaline serine protease
34
C
X17561



Asp o 21
TAKA-amylase A
53
C
D00434, M33218


Penicillium brevicompactum
Pen b 13
alkaline serine protease
33

86A


Penicillium chrysogenum
Pen ch 13
alkaline serine protease
34

87


(formerly P. notatum)
Pen ch 18
vacuolar serine protease
32

87



Pen ch 20
N-acetyl glucosaminidas
68

87A


Penicillium citrinum
Pen c 3
peroxisomal mem. prot.
18

86B



Pen c 13
alkaline serine protease
33

86A



Pen c 19
heat shock prot. P70
70
C
U64207



Pen c 22w
enolase
46
C
AF254643



Pen c 24
elongation factor 1 beta

C
AY363911


Penicillium oxalicum
Pen o 18
vacuolar serine protease
34

87B


1.3 Hypocreales


Fusarium culmorum
Fus c 1
ribosomal prot. P2
11* 
C
AY077706



Fus c 2
thioredoxin-like prot.
13* 
C
AY077707


1.4 Onygenales


Trichophyton rubrum
Tri r 2


C
88



Tri r 4
serine protease

C
88


Trichophyton tonsurans
Tri t 1

30
P
88A



Tri t 4
serine protease
83
C
88


1.5 Saccharomycetales


Candida albicans
Cand a 1

40
C
89



Cand a 3
peroxisomal protein
29
C
AY136739


Candida boidinii
Cand b 2

20
C
J04984, J04985


2. Basidiomycotina


2.1 Hymenomycetes


Psilocybe cubensis
Psi c 1



Psi c 2
cyclophilin
16

89A


Coprinus comatus
Cop c 1
leucine zipper protein
11
C
AJ132235


shaggy cap
Cop c 2



AJ242791



Cop c 3



AJ242792



Cop c 5



AJ242793



Cop c 7



AJ242794


2.2 Urediniomycetes


Rhodotorula mucilaginosa
Rho m 1
enolase
47
C
89B



Rho m 2
vacuolar serine protease
31
C
AY547285


2.3 Ustilaginomycetes


Malassezia furfur
Mala f 2
MF1, peroxisomal
21
C
AB011804, 90




membrane protein



Mala f 3
MF2, peroxisomal
20
C
AB011805, 90




membrane protein



Mala f 4
mitochondrial malate dehydrogenase
35
C
AF084828, 90A


Malassezia sympodialis
Mala s 1


C
X96486, 91



Mala s 5

18* 
C
AJ011955



Mala s 6

17* 
C
AJ011956



Mala s 7


C
AJ011957, 91A



Mala s 8

19* 
C
AJ011958, 91A



Mala s 9

37* 
C
AJ011959, 91A



Mala s 10
heat shock prot. 70
86
C
AJ428052



Mala s 11
Mn superoxide dismut.
23
C
AJ548421


3. Deuteromycotina


3.1 Tuberculariales


Epicoccum purpurascens
Epi p 1
serine protease
30
P
SW: P83340, 91B


(formerly E. nigrum)


G. Insects


Aedes aegyptii
Aed a 1
apyrase
68
C
L12389


mosquito
Aed a 2

37
C
M33157


Apis mellifera
Api m 1
phospholipase A2
16
C
92


honey bee
Api m 2
hyaluronidase
44
C
93



Api m 4
melittin
3
C
94



Api m 6

7-8
P
Kettner p.c.



Api m 7
CUB serine protease
39
C
AY127579


Bombus pennsylvanicus
Bom p 1
phospholipase
16
P
95


bumble bee
Bom p 4
protease

P
95


Blattella germanica
Bla g 1
Bd90k

C


German cockroach
Bla g 2
aspartic protease
36
C
96



Bla g 4
calycin
21
C
97



Bla g 5
glutathione transferase
22
C
98



Bla g 6
troponin C
27
C
98


Periplaneta americana
Per a 1
Cr-PII

C


American cockroach
Per a 3
Cr-PI
72-78
C
98A



Per a 7
tropomyosin
37
C
Y14854


Chironomus kiiensis
Chi k 10
tropomyosin
32.5*
C
AJ012184


midge


Chironomus thummi thummi
Chi t 1-9
hemoglobin
16
C
99


midge
Chi t 1.01
component III
16
C
P02229



Chi t 1.02
component IV
16
C
P02230



Chi t 2.0101
component I
16
C
P02221



Chi t 2.0102
component IA
16
C
P02221



Chi t 3
component II-beta
16
C
P02222



Chi t 4
component IIIA
16
C
P02231



Chi t 5
component VI
16
C
P02224



Chi t 6.01
component VIIA
16
C
P02226



Chi t 6.02
component IX
16
C
P02223



Chi t 7
component VIIB
16
C
P02225



Chi t 8
component VIII
16
C
P02227



Chi t 9
component X
16
C
P02228


Ctenocephalides felis felis
Cte f 1


cat flea
Cte f 2
M1b
27
C
AF231352



Cte f 3

25
C


Thaumetopoea pityocampa
Tha p 1

15
P
PIR: A59396, 99A


pine processionary moth


Lepisma saccharina
Lep s 1
tropomyosin
36
C
AJ309202


silverfish


Dolichovespula maculata
Dol m 1
phospholipase A1
35
C
100


white face hornet
Dol m 2
hyaluronidase
44
C
101



Dol m 5
antigen 5
23
C
102, 103


Dolichovespula arenaria
Dol a 5
antigen 5
23
C
104


yellow hornet


Polistes annularies
Pol a 1
phospholipase A1
35
P
105


wasp
Pol a 2
hyaluronidase
44
P
105



Pol a 5
antigen 5
23
C
104


Polistes dominulus
Pol d 1



Hoffman p.c.


Mediterranean paper wasp
Pol d 4
serine protease
32-34
C
Hoffman p.c.



Pol d 5



P81656


Polistes exclamans
Pol e 1
phospholipase A1
34
P
107


wasp
Pol e 5
antigen 5
23
C
104


Polistes fuscatus
Pol f 5
antigen 5
23
C
106


wasp


Polistes gallicus
Pol g 5
antigen 5
24
C
P83377


wasp


Polistes metricus
Pol m 5
antigen 5
23
C
106


wasp


Vespa crabo
Vesp c 1
phospholipase
34
P
107


European hornet
Vesp c 5
antigen 5
23
C
106


Vespa mandarina
Vesp m 1



Hoffman p.c.


giant asian hornet
Vesp m 5



P81657


Vespula flavopilosa
Ves f 5
antigen 5
23
C
106


yellowjacket


Vespula germanica
Ves g 5
antigen 5
23
C
106


yellowjacket


Vespula maculifrons
Ves m 1
phospholipase A1
33.5
C
108


yellowjacket
Ves m 2
hyaluronidase
44
P
109



Ves m 5
antigen 5
23
C
104


Vespula pennsylvanica
Ves p 5
antigen 5
23
C
106


yellowjacket


Vespula squamosa
Ves s 5
antigen 5
23
C
106


yellowjacket


Vespula vidua
Ves vi 5
antigen 5
23
C
106


wasp


Vespula vulgaris
Ves v 1
phospholipase A1
35
C
105A


yellowjacket
Ves v 2
hyaluronidase
44
P
105A



Ves v 5
antigen 5
23
C
104


Myrmecia pilosula
Myr p 1


C
X70256


Australian jumper ant
Myr p 2


C
S81785


Solenopsis geminata
Sol g 2



Hoffman p.c.


tropical fire ant
Sol g 4



Hoffman p.c.


Solenopsis invicta
Sol i 2

13
C
110, 111


fire ant
Sol i 3

24
C
110



Sol i 4

13
C
110


Solenopsis saevissima
Sol s 2



Hoffman p.c.


Brazilian fire ant


Triatoma protracta
Tria p 1
Procalin
20
C
AF179004, 111A.


California kissing bug


H. Foods


Gadus callarias
Gad c 1
allergen M
12
C
112, 113


cod


Salmo salar
Sal s 1
parvalbumin
12
C
X97824


Atlantic salmon


Bos domesticus
Bos d 4
alpha-lactalbumin
14.2
C
M18780


domestic cattle
Bos d 5
beta-lactoglobulin
18.3
C
X14712


(milk)
Bos d 6
serum albumin
67
C
M73993


see also animals
Bos d 7
immunoglobulin
160

77



Bos d 8
caseins
20-30

77


Cyprinus carpio
Cyp c 1
parvalbumin
12
C
129


(Common carp)


Gallus domesticus
Gal d 1
ovomucoid
28
C
114, 115


chicken
Gal d 2
ovalbumin
44
C
114, 115



Gal d 3
Ag22, conalbumin
78
C
114, 115



Gal d 4
lysozyme
14
C
114, 115



Gal d 5
serum albumin
69
C
X60688


Metapenaeus ensis
Met e 1
tropomyosin

C
U08008


shrimp


Penaeus aztecus
Pen a 1
tropomyosin
36
P
116


shrimp


Penaeus indicus
Pen i 1
tropomyosin
34
C
116A


shrimp


Penaeus monodon
Pen m 1
tropomyosin
38
C


black tiger shrimp
Pen m 2
arginine kinase
40
C
AF479772, 117


Todarodes pacificus
Tod p 1
tropomyosin
38
P
117A


squid


Helix aspersa
Hel as 1
tropomyosin
36
C
Y14855, 117B


brown garden snail


Haliotis midae
Hal m 1

49

117C


abalone


Rana esculenta
Ran e 1
parvalbumin alpha
11.9*
C
AJ315959


edible frog
Ran e 2
parvalbumin beta
11.7*
C
AJ414730


Brassica juncea
Bra j 1
2S albumin
14
C
118


oriental mustard


Brassica napus
Bra n 1
2S albumin
15
P
118A, P80208


rapeseed


Brassica rapa
Bra r 2
hom: prohevein
25

P81729


turnip


Hordeum vulgare
Hor v 15
BMAI-1
15
C
119


barley
Hor v 16
alpha-amylase



Hor v 17
beta-amylase



Hor v 21
gamma-3 hordein
34
C
119A,







SW: P80198


Secale cereale
Sec c 20
secalin


see isoall. list


rye


Triticum aestivum
Tri a 18
agglutinin


wheat
Tri a 19
omega-5 gliadin
65
P
PIR: A59156


Zea mays
Zea m 14
lipid transfer prot.
9
P
P19656


maise, corn


Oryza sativa
Ory s 1


C
119B, U31771


rice


Apium graveolens
Api g 1
hom: Bet v 1
16* 
C
Z48967


celery
Api g 4
profilin


AF129423



Api g 5

55/58
P
P81943


Daucus carota
Dau c 1
hom: Bet v 1
16
C
117D, see isoallergen


carrot
Dau c 4
profilin

C
AF456482


list


Corylus avellana
Cor a 1.04
hom: Bet v 1
17
C
see list of isoallergens


hazelnut
Cor a 2
profilin
14
C
AF327622



Cor a 8
lipid transfer protein
9
C
AF329829


Malus domestica
Mal d 1
hom: Bet v 1

C
see list of isoallergens


apple
Mal d 2
hom: thaumatin

C
AJ243427



Mal d 3
lipid transfer protein
9
C
Pastorello p.c.



Mal d 4
profilin
14.4*
C
see list of isoallergens


Pyrus communis
Pyr c 1
hom: Bet v 1
18
C
AF05730


pear
Pyr c 4
profilin
14
C
AF129424



Pyr c 5
hom: isoflavone reductas
33.5
C
AF071477


Persea americana
Pers a 1
endochitinase
32
C
Z78202


avocado


Prunus armeniaca
Pru ar 1
hom: Bet v 1

C
U93165


apricot
Pru ar 3
lipid transfer protein
9
P


Prunus avium
Pru av 1
hom: Bet v 1

C
U66076


sweet cherry
Pru av 2
hom: thaumatin

C
U32440



Pru av 3
lipid transfer protein
10
C
AF221501



Pru av 4
profilin
15
C
AF129425


Prunus domestica
Pru d 3
lipid transfer protein
9
P
119C


European plum


Prunus persica
Pru p 3
lipid transfer protein
10
P
P81402


peach
Pru p 4
profilin
14
C
see isoallergen list


Asparagus officinalis
Aspa o 1
lipid transfer protein
9
P
119D


Asparagus


Crocus sativus
Cro s 1

21

Varasteh A-R p.c.


saffron crocus


Lactuca sativa
Lac s 1
lipid transfer protein
9

Vieths p.c.


lettuce


Vitis vinifera
Vit v 1
lipid transfer protein
9
P
P80274


grape


Musa x paradisiaca
Mus xp 1
profilin
15
C
AF377948


banana


Ananas comosus
Ana c 1
profilin
15
C
AF377949


pineapple
Ana c 2
bromelain
22.8*
C
119E-G, D14059


Citrus limon
Cit l 3
lipid transfer protein
9
P
Torrejon p.c.


lemon


Citrus sinensis
Cit s 1
germin-like protein
23
P
Torrejon p.c.


sweet orange
Cit s 2
profilin
14
P
Torrejon p.c.



Cit s 3
lipid transfer protein
9
P
Torrejon p.c.


Litchi chinensis
Lit c 1
profilin
15
C
AY049013


litchi


Sinapis alba
Sin a 1
2S albumin
14
C
120


yellow mustard


Glycine max
Gly m 1
HPS
7
P
120A


soybean
Gly m 2

8
P
A57106



Gly m 3
profilin
14
C
see list of isoallergens



Gly m 4
(SAM22) PR-10 prot.
17
C
X60043, 120B


Vigna radiata
Vig r 1
PR-10 protein
15
C
AY792956


mung bean


Arachis hypogaea
Ara h 1
vicilin
63.5
C
L34402


peanut
Ara h 2
conglutin
17
C
L77197



Ara h 3
glycinin
60
C
AF093541



Ara h 4
glycinin
37
C
AF086821



Ara h 5
profilin
15
C
AF059616



Ara h 6
hom: conglutin
15
C
AF092846



Ara h 7
hom: conglutin
15
C
AF091737



Ara h 8
PR-10 protein
17
C
AY328088


Lens culinaris
Len c 1
vicilin
47
C
see list of isoallergens


lentil
Len c 2
seed biotinylated prot.
66
P
120C


Pisum savitum
Pis s 1
vicilin
44
C
see list of isoallergens


pea
Pis s 2
convicilin
63
C
pending


Actinidia chinensis
Act c 1
cysteine protease
30
P
P00785


kiwi
Act c 2
thaumatin-like protein
24
P
SW: P81370, 121


Capsicum annuum
Cap a 1w
osmotin-like protein
23
C
AJ297410


bell pepper
Cap a 2
profilin
14
C
AJ417552


Lycopersicon esculentum
Lyc e 1
profilin
14
C
AJ417553


tomato
Lyc e 2
b-fructofuranosidase
50
C
see isoallergen list



Lyc e 3
lipid transfer prot.
6
C
U81996


Solanum tuberosum
Sola t 1
patatin
43
P
P15476


potato
Sola t 2
cathepsin D inhibitor
21
P
P16348



Sola t 3
cysteine protease inhibitor
21
P
P20347



Sola t 4
aspartic protease inhibitor
16 + 4
P
P30941


Bertholletia excelsa
Ber e 1
2S albumin
9
C
P04403, M17146


Brazil nut
Ber e 2
11S globulin seed storage protein
29
C
AY221641


Juglans nigra
Jug n 1
2S albumin
19* 
C
AY102930


black walnut
Jug n 2
vicilin-like prot.
56* 
C
AY102931


Juglans regia
Jug r 1
2S albumin

C
U66866


English walnut
Jug r 2
vicilin
44
C
AF066055



Jug r 3
lipid transfer protein
9
P
Pastorello


Anacardium occidentale
Ana o 1
vicilin-like protein
50
C
see isoallergen list


Cashew
Ana o 2
legumin-like protein
55
C
AF453947



Ana o 3
2S albumin
14
C
AY081853


Ricinus communis
Ric c 1
2S albumin

C
P01089


Castor bean


Sesamum indicum
Ses i 1
2S albumin
9
C
121A, AF240005


sesame
Ses i 2
2S albumin
7
C
AF091841



Ses i 3
7S vicilin-like globulin
45
C
AF240006



Ses i 4
oleosin
17
C
AAG23840



Ses i 5
oleosin
15
C
AAD42942


Cucumis melo
Cuc m 1
serine protease
66
C
D32206


muskmelon
Cuc m 2
profilin
14
C
AY271295



Cuc m 3
pathogenesis-rel p. PR-1
16* 
P
P83834


I. Others


Anisakis simplex
Ani s 1

24
P
121B, A59069


nematode
Ani s 2
paramyosin
97
C
AF173004



Ani s 3
tropomyosin
41
C
121C, Y19221



Ani s 4

9
P
P83885


Argas reflexus
Arg r 1

17
C
AJ697694


pigeon tick


Ascaris suum
Asc s 1

10
P
122


worm


Carica papaya
Car p 3w
papain
23.4*
C
122A, M15203


papaya


Dendronephthya nipponica
Den n 1

53
P
122B


soft coral


Hevea brasiliensis
Hev b 1
elongation factor
58
P
123, 124


rubber (latex)
Hev b 2
1,3-glucanase
34/36
C
125



Hev b 3

24
P
126, 127



Hev b 4
component of
100-115
P
128




microhelix complex



Hev b 5

16
C
U42640



Hev b 6.01
hevein precursor
20
C
M36986, p02877



Hev b 6.02
hevein
5
C
M36986, p02877



Hev b 6.03
C-terminal fragment
14
C
M36986, p02877



Hev b 7.01
hom: patatin from B-serum
42
C
U80598



Hev b 7.02
hom: patatin from C-serum
44
C
AJ223038



Hev b 8
profilin
14
C
see list of isoallergens



Hev b 9
enolase
51
C
AJ132580



Hev b 10
Mn superoxide dismut.
26
C
see list of isoallergens



Hev b 11
class 1 chitinase

C
see list of isoallergens



Hev b 12
lipid transfer protein
9.3
C
AY057860



Hev b 13
esterase
42
P
P83269


Homo sapiens
Hom s 1

73* 
C
Y14314


human autoallergens
Hom s 2

10.3*
C
X80909



Hom s 3

20.1*
C
X89985



Hom s 4

36* 
C
Y17711



Hom s 5

42.6*
C
P02538


Triplochiton scleroxylon
Trip s 1
class 1 chitinase
38.5
P
Kespohl p.c.


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REFERENCES


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According to a preferred embodiment of the present invention the hypoallergenic molecule exhibits reduced IgE-binding capacity.
According to another preferred embodiment of the present invention the hypoallergenic molecule exhibits reduced T-cell reactivity.
However, also allergen fragments comprising at least one T-cell epitope may be used in the hypoallergenic protein according to the present invention.
“Exhibiting reduced IgE-binding capacity”, as used herein, means that the molecules according to the present invention show significantly reduced IgE-binding capacity or activity (at least 50% less, preferably at least 70% less, more preferably at least 80% less, even more preferably at least 90% less, most preferably at least 95% less, binding capacity compared to the wild-type allergen) or even lack them at all.
IgE-binding activity/capacity of molecules like peptides and proteins can be determined by, for example, an enzyme linked immunosorbent assay (ELISA) using, for example, sera obtained from a subject, (i.e., an allergic subject) that has been previously exposed to the wild-type allergen. Briefly, a peptide to be tested is coated onto wells of a microtiter plate. After washing and blocking the wells, an antibody solution consisting of the plasma of an allergic subject, who has been exposed to the peptide being tested or the protein from which it was derived, is incubated in the wells. A labelled secondary antibody is added to the wells and incubated. The amount of IgE-binding is then quantified and compared to the amount of IgE bound by a purified wild-type allergen.
Alternatively, the binding activity of a peptide can be determined by Western blot analysis. For example, a peptide to be tested is run on a polyacrylamide gel using SDS-PAGE. The peptide is then transferred to nitrocellulose and subsequently incubated with serum from an allergic subject. After incubation with the labelled secondary antibody, the amount of IgE bound is determined and quantified.
Another assay which can be used to determine IgE-binding activity of a peptide is a competition ELISA assay. Briefly, an IgE-antibody pool is generated by combining plasma from allergic subjects who have been shown by direct ELISA to be IgE-reactive with wild-type allergen. This pool is used in ELISA competition assays to compare IgE-binding to wild-type allergen to the peptide tested. IgE-binding for the wild-type allergen and the peptide being tested is determined and quantified.
A “T-cell epitope” means a protein (e.g., allergen) or fragment thereof, for which a T-cell has an antigen specific binding site, the result of binding to said binding site activates the T-cell. The term “exhibiting reduced T-cell reactivity”, as used herein, refers to molecules which exhibit a T-cell reactivity which is significantly reduced compared to the stimulation induced by the wild-type allergen from which the hypoallergenic molecule is derivedusing equimolar amounts in standard assays known in the art (reduced T-cell reactivity means at least 30%, preferably at least 50%, more preferably at least 70%, most preferably at least 90%, less stimulation of hypoallergenic molecules compared to the wildtype allergen at equimolar amounts). In a particular preferred embodiment of this invention, the molecules may “lack” T-cell epitopes and thus molecule shows reduced T-cell reactivity in the individual(s) to be treated (i.e., who is to receive an epitope-presenting valency platform molecule). It is likely that, for example, an allergen-derived molecule may lack a T-cell epitope(s) with respect to an individual, or a group of individuals, while possessing a T-cell epitope(s) with respect to other individual(s). Methods for detecting the presence of a T-cell epitope are known in the art and include assays which detect T-cell proliferation (such as thymidine incorporation). Immunogens that fail to induce statistically significant incorporation of thymidine above background (i.e., generally p less than 0.05 using standard statistically methods) are generally considered to lack T-cell epitopes, although it will be appreciated that the quantitative amount of thymidine incorporation may vary, depending on the immunogen being tested (see, e.g., Zhen L. et al. (Infect Immun. (2003) 71:3920-3926)). Generally, a stimulation index below about 2-3, more preferably less than about 1, indicates lack of T-cell reactivity and epitopes. The presence of T-cell epitopes can also be determined by measuring secretion of T-cell-derived lymphokines according to standard methods. The stimulation index (SI) may be calculated by dividing the proliferation rate (Thymidine uptake) of stimulated cells through the proliferation rate of unstimulated cells in medium alone. SI=1 means no stimulation, SI<1 indicates toxic effects and SI>1 indicates stimulation of cells. Location and content of T-cell epitopes, if present, can be determined empirically.
The cytokine secretion may be determined in addition to the stimulation of T cells. For example, IFN-gamma has been recognized as a harmful cytokine. Other examples may be TNF-alpha, IL-5, IL-4, IL-8 etc.
The allergen fragment is preferably composed of amino acids 151 to 177, 87 to 117, 1 to 30, 43 to 70 or 212 to 241 of Phl p 1, amino acids 93 to 128, 98 to 128, 26 to 53, 26 to 58, 132 to 162, 217 to 246, 252 to 283 or 176 to 212 of Phl p 5, amino acids 1 to 34 or 35 to 70 of chain 1 of Fel d 1, amino acids 1 to 34, 35 to 63 or 64 to 92 of chain 2 of Fel d 1, amino acids 30 to 59, 50 to 79 or 75 to 104 of Bet v 1, amino acids 1 to 33, 21 to 51, 42 to 73, 62 to 103 or 98 to 129 of Der p 2, amino acids 1 to 30, 20 to 50, 50 to 80, 90 to 125, 125 to 155 or 165 to 198 of Der p 7, amino acids 1-35, 36-70, 71-110, 111-145, 140-170, 175-205, 210-250 or 250-284 of Der p 10, amino acids 1 to 35, 35 to 72, 70 to 100 or 90 to 122 of Der p 21, amino acids 1 to 32, 15 to 48 or 32 to 70 of Clone 30, amino acids 19 to 58, 59 to 95, 91 to 120 or 121 to 157 of Alt a 1, amino acids 31 to 60, 45 to 80, 60 to 96 or 97 to 133 of Par j 2, amino acids 1 to 40, 36 to 66, 63 to 99, 86 to 120 or 107 to 145 of Ole e 1, amino acids 25 to 58, 99 to 133, 154 to 183, 277 to 307, 334 to 363, 373 to 402, 544 to 573, 579 to 608, 58 to 99, 125 to 165, 183 to 224, 224 to 261, 252 to 289, 303 to 340, 416 to 457, 460 to 500 or 501 to 542 of Fel d 2, amino acids 19 to 58, 52 to 91, 82 to 119, 106 to 144 or 139 to 180 of Can f 2, amino acids 19 to 56, 51 to 90, 78 to 118, 106 to 145 or 135-174 of Can f 1, amino acids 27 to 70, 70 to 100 or 92 to 132 of Art v 1, amino acids 31 to 70, 80 to 120, 125 to 155, 160 to 200, 225 to 263, 264 to 300 305 to 350 or 356 to 396 of Amb a 1, amino acids 1 to 34, 35 to 74, 74 to 115, 125 to 165, 174 to 213, 241 to 280, 294 to 333, 361 to 400 or 401 to 438 of Alt a 6, amino acids 1 to 40, 41 to 80, 81 to 120, 121 to 160 of Alt a 2 or fragments or sequence variations thereof.
The specific amino acid sequences of the above identified allergen-derived molecules are:













Peptide
Position
Sequence
SEQ ID No.



















Pep Alt a 1.1
19-58
APLESRQDTASCPVTTEGDYVWKISEFYGRKPEG-
23





TYYNSL


 


Pep Alt a 1.2
59-95
GFNIKATNGGTLDFTCSAQADKLEDHKWYSCGENSFM
24


 


Pep Alt a 1.3
 91-120
ENSFMDFSFDSDRSGLLLKQKVSDDITYVA
25


 


Pep Alt a 1.4
121-157
TATLPNYCRAGGNGPKDFVCQGVADAYITLVTLPKSS
26


 


Pep Alt a 2.1
 1-40
MHSSNNFFKDNIFRSLSKEDPDYSRNIEGQVIRLH-
27




WDWAQ


 


Pep Alt a 2.2
41-80
LLMLSAKRMKVAFKLDIEKDQRVWDRCTADDLK-
28




GRNGFKR


 


Pep Alt a 2.3
 81-120
CLQFTLYRPRDLLSLLNEAFFSAFRENRETIINTD-
29




LEYAA


 


Pep Alt a 2.4
121-160
KSISMARLEDLWKEYQKIFPSIQVITSAFRSIE-
30




PELTVYT


 


Pep Alt a 2.5
161-190
CLKKIEASFELIEENGDPKITSEIQLLKAS
31


 


Pep Alt a 6.1
 1-34
MTITKIHARSVYDSRGNPTVEVDIVTETGLHRAI
32


 


Pep Alt a 6.2
35-74
VTETGLHRAIVPSGASTGSHEACELRDGDKSKWG-
33




GKGVTK


 


Pep Alt a 6.3
 74-115
APALIKEKLDVKDQSAVDAFLNKLDGTTNKTNL-
34




GANAILGVS


 


Pep Alt a 6.4
125-165
EKGVPLYAHISDLAGT KKPYVLPVPF QNVLNG-
35




GSHAGGRLA


 


Pep Alt a 6.5
174-213
CEAPTFSEAMRQGAEVYQKLKALAKKTYGQSAGN-
36




VGDEGG


 


Pep Alt a 6.6
241-280
IKIAMDVASSEFYKADEKKYDLDFKNPDSDKSKWL-
37




TYEQL


 


Pep Alt a 6.7
294-333
VSIEDPFAEDDWEAWSYFFKTYDGQIVGDDLTVT-
38




NPEFIK


 


Pep Alt a 6.8
361-400
AKDAFGAGWGVMVSHRSGETEDVTIADIVVGLRS-
39




GQIKTG


 


Pep Alt a 6.9
401-438
APARSERLAKLNQILRIEEELGDNAVYAGNNFR-
40




TAVNL


 


Pep Amb a 1.1
31-70
EILPVNETRRLTTSGAYNIIDGCWRGKADWAEN-
41




RKALADC


 


Pep Amb a 1.2
 80-120
GGKDGDIYTVTSELDDDVANPKEGTLRFGAAQNR-
42




PLWIIFE


 


Pep Amb a 1.3
125-155
IRLDKEMVVNSDKTIDGRGAKVEIINAGFTL
43


 


Pep Amb a 1.4
160-200
NVIIHNINMHDVKVNPGGLIKSNDGPAAPRAGSDG-
44




DAISIS


 


Pep Amb a 1.5
225-263
GTTRLTVSNSLFTQHQFVLLFGAGDENIEDRGMLAT-
45




VAF


 


Pep Amb a 1.6
264-300
NTFTDNVDQRMPRCRHGFFQVVNNNYDKWGSYAIGGS
46


 


Pep Amb a 1.7
305-350
ILSQGNRFCAPDERSKKNVLGRHGEAAAESMKWN-
47




WRTNKDVLENGA


 


Pep Amb a 1.8
356-396
GVDPVLTPEQSAGMIPAEPGESALSLTSSAGVLSC-
48




QPGAPC


 


Pep Art v 1.1
27-70
SKLCEKTSKTYSGKCDNKKCDKKCIEWEKAQHGACH-
49




KREAGKES


 


Pep Art v 1.2
 70-100
SCFCYFDCSKSPPGATPAPPGAAPPPAAGGS
50


 


Pep Art v 1.3
 92-132
APPPAAGGSPSPPADGGSPPPPADGGSPPVDGG-
51




SPPPPSTH


 


Can f 1 Pep 1
19-56
QDTPALGKDTVAVSGKWYLKAMTADQEVPEKPDSVT-
52




PM


 


Can f 1 Pep 2
51-90
DSVTPMILKAQKGGNLEAKITMLTNGQCQNITVVL-
53




HKTSE


 


Can f 1 Pep 3
 78-118
CQNITVVLHKTSEPGKYTAYEGQRVVFIQPSPVRD-
54




HYILYC


 


Can f 1 Pep 4
106-145
QPSPVRDHYILYCEGELHGRQIRMAKLLGRD-
55




PEQSQEALE


 


Can f 1 Pep 5
135-174
RDPEQSQEALEDFREFSRAKGLNQEILELAQSETC-
56




SPGGQ


 


Can f 2 Pep 1
19-58
QEGNHEEPQGGLEELSGRWHSVALASNKSDLIKP-
57




WGHFRV


 


Can f 2 Pep 2
52-91
PWGHFRVFIHSMSAKDGNLHGDILIPQDGQCEK-
58




VSLTAFK


 


Can f 2 Pep 3
 82-119
CEKVSLTAFKTATSNKFDLEY-
59




WGHNDLYLAEVDPKSYL


 


Can f 2 Pep 4
106-144
NDLYLAEVDPKSYLILYMINQYN-
60




DDTSLVAHLMVRDLSR


 


Can f 2 Pep 5
139-180
VRDLSRQQDFLPAFESVCEDIGLHKDQIVVLS-
61




DDDRCQGSRD


 


Fel d 2 Pep 1
25-58
EAHQSEIAHRFNDLGEEHFRGLVLVAFSQYLQQC
62


 


Fel d 2 Pep 2
 99-133
CTVASLRDKYGEMADCCEKKEPERNECFLQHKDDN
63


 


Fel d 2 Pep 3
154-183
NEQRFLGKYLYEIARRHPYFYAPELLYYAE
64


 


Fel d 2 Pep 4
277-307
CADDRADLAKYICENQDSISTKLKECCGKPV
65


 


Fel d 2 Pep 5
334-363
VEDKEVCKNYQEAKDVFLGTFLYEYSRRHP
66


 


Fel d 2 Pep 6
373-402
LAKEYEATLEKCCATDDPPACYAHVFDEFK
67


 


Fel d 2 Pep 7
544-573
EKQIKKQSALVELLKHKPKATEEQLKTVMG
68


 


Fel d 2 Pep 8
579-608
VDKCCAAEDKEACFAEEGPKLVAAAQAALA
69


 


Fel d 2 Pep 9
58-99
CPFEDHVKLVNEVTEFAKGCVADQSAANCEK-
70




SLHELLGDKLC


 


Fel d 2 Pep 10
125-165
CFLQHKDDNPGFGQLVTPEADAMCTAFHENEQRFLG-
71




KYLYE


 


Fel d 2 Pep 11
183-224
EEYKGVFTECCEAADKAACLTPKVDALREKVLAS-
72




SAKERLKC


 


Fel d 2 Pep 12
224-261
CASLQKFGERAFKAWSVARLSQKFPKAE-
73




FAEISKLVTD


 


Fel d 2 Pep 13
252-289
FAEISKLVTDLAKIHKECCHGDLLECADDRADLAKY-
74




IC


 


Fel d 2 Pep 14
303-340
CGKPVLEKSHCISEVERDELPADLPPLAVD-
75




FVEDKEVC


 


Fel d 2 Pep 15
416-457
CELFEKLGEYGFQNALLVRYTKKVPQVST-
76




PTLVEVSRSLGKV


 


Fel d 2 Pep 16
460-500
CTHPEAERLSCAEDYLSVVLNRLCVLHEKTPVSER-
77




VTKC


 


Fel d 2 Pep 17
501-542
CTESLVNRRPCFSALQVDETYVPKEFSAETFTF-
78




HADLCTLPE


 


Pep Ole e 1.1
 1-40
EDIPQPPVSQFHIQGQVYCDTCRAGFITELSEFIP-
79




GASLR


 


Pep Ole e 1.2
36-66
GASLRLQCKDKENGDVTFTEVGYTRAEGLYS
80


 


Pep Ole e 1.3
63-99
GLYSMLVERDHKNEFCEITLISSGRKDCNEIPTEGWA
81


 


Pep Ole e 1.4
 86-120
GRKDCNEIPTEGWAKPSLKFKLNTVNGTTRTVNPL
82


 


Pep Ole e 1.5
107-145
LNTVNGTTRTVNPLGFFKKEALPKCAQVYNKL-
83




GMYPPNM


 


Pep Par j 2.1
31-60
GEEACGKVVQDIMPCLHFVKGEEKEPSKEC
84


 


Pep Par j 2.2
45-80
CLHFVKGEEKEPSKECCSGTKKLSEEVKTTEQKREA
85


 


Pep Par j 2.3
60-96
CCSGTKKLSEEVKTTEQKREACKCIVRATKGISGIKN
86


 


Pep Par j 2.4
 97-133
ELVAEVPKKCDIKTTLPPITADFDCSKIQSTIFRGYY
87


 


Der p 1 Pep 1
 1-30
TNACSINGNAPAEIDLRQMRTVTPIRMQGG
88


 


Der p 1 Pep 2
52-84
NQSLDLAEQELVDCASQHGCHGDTIPRGIEYIQ
89


 


Der p 1 Pep 3
 85-115
HNGVVQESYYRYVAREQSCRRPNAQRFGISN
90


 


Der p 1 Pep 4
 99-135
REQSCRRPNAQRFGISNYCQIYPPNVNKIREALAQTH
91


 


Der p 1 Pep 5
145-175
KDLDAFRHYDGRTIIQRDNGYQPNYHAVNIV
92


 


Der p 1 Pep 6
155-187
GRTIIQRDNGYQPNYHAVNIVGYSNAQGVDYWI
93


 


Der p 1 Pep 7
175-208
VGYSNAQGVDYWIVRNSWDTNWGDNGYGYFAANI
94


 


Der p 1 Pep 8
188-222
VRNSWDTNWGDNGYGYFAANIDLMMIEEYPYVVIL
95


 


Der p 2 Pep 1
 1-33
DQVDVKDCANHEIKKVLVPGCHGSEPCIIHRGK
96


 


Der p 2 Pep 2
21-51
CHGSEPCIIHRGKPFQLEAVFEANQNSKTAK
97


 


Der p 2 Pep 3
42-73
EANQNSKTAKIEIKASIEGLEVDVPGIDPNAC
98


 


Der p 2 Pep 4
 62-103
EVDVPGIDPNACHYMKCPLVKGQQYDIKYTWIVP-
99




KIAPKSEN


 


Der p 2 Pep 5
 98-129
APKSENVVVTVKVMGDNGVLACAIATHAKIRD
100


 


Der p 5 Pep 1
 1-35
MEDKKHDYQNEFDFLLMERIHEQIKKGELALFYLQ
101


 


Der p 5 Pep 2
25-60
KKGELALFYLQEQINHFEEKPTKEMKDKIVAEMDTI
102


 


Der p 5 Pep 3
65-95
DGVRGVLDRLMQRKDLDIFEQYNLEMAKKSG
103


 


Der p 5 Pep 4
 78-114
DLDIFEQYNLEMAKKSGDILERDLKKEEARVKKIEV
104


 


Der p 7 Pep 1
 1-30
DPIHYDKITEEINKAVDEAVAAIEKSETFD
105


 


Der p 7 Pep 2
20-50
VAAIEKSETFDPMKVPDHSDKFERHIGIIDL
106


 


Der p 7 Pep 3
50-80
LKGELDMRNIQVRGLKQMKRVGDANVKSEDG
107


 


Der p 7 Pep 4
 90-125
VHDDVVSMEYDLAYKLGDLHPNTHVISDIQDFVVEL
108


 


Der p 7 Pep 5
125-155
LSLEVSEEGNMTLTSFEVRQFANVVNHIGGL
109


 


Der p 7 Pep 6
165-198
LSDVLTAIFQDTVRAEMTKVLAPAFKKELERNNQ
110


 


Der p 10 Pep 1
 1-35
MEAIKKKMQAMKLEKDNAIDRAEIAEQKARDANLR
111


 


Der p 10 Pep 2
36-70
AEKSEEEVRALQKKIQQIENELDQVQEQLSAANTK
112


 


Der p 10 Pep 3
 71-110
LEEKEKALQTAEGDVAALNRRIQLIEEDLERSEER-
113




LKIAT


 


Der p 10 Pep 4
111-145
AKLEEASQSADESERMRKMLEHRSITDEERMEGLE
114


 


Der p 10 Pep 5
140-170
RMEGLENQLKEARMMAEDADRKYDEVARKLA
115


 


Der p 10 Pep 6
175-205
DLERAEERAETGESKIVELEEELRVVGNNLK
116


 


Der p 10 Pep 7
210-250
SEEKAQQREEAHEQQIRIMTTKLKEAEARAEFAERS-
117




VQKLQ


 


Der p 10 Pep 8
250-284
QKEVDRLEDELVHEKEKYKSISDELDQTFAELTGY
118


 


Der p 21 Pep 1
 1-35
MFIVGDKKEDEWRMAFDRLMMEELETKIDQVEKGL
119


 


Der p 21 Pep 2
35-72
LHLSEQYKELEKTKSKELKEQILRELTIGENFMKGAL
120


 


Der p 21 Pep 3
 70-100
GALKFFEMEAKRTDLNMFERYNYEFALESIK
121


 


Der p 21 Pep 4
 90-122
YNYEFALESIKLLIKKLDELAKKVKAVNPDEYY
122


 


Clone 30 Pep 1
 1-32
MANDNDDDPTTTVHPTTTEQPDDKFECPSRFG
123


 


Clone 30 Pep 2
15-48
PTTTEQPDDKFECPSRFGYFADPKDPHKFYICSN
124


 


Clone 30 Pep 3
32-70
GYFADPKDPHKFYICSNWEAVHKDCPGNTRWNEDEE
125




TCT


 


Bet v 1 Pep 1
30-59
LFPKVAPQAISSVENIEGNGGPGTIKKISF
126


 


Bet v 1 Pep 2
50-79
GPGTIKKISFPEGFPFKYVKDRVDEVDHTN
127


 


Bet v 1 Pep 3
 75-104
VDHTNFKYNYSVIEGGPIGDTLEKISNEIK
128


 


Fel d 1 chain
 1-34
EICPAVKRDVDLFLTGTPDEYVEQVAQYKALPVVC
129


1 Pep 1


 


Fel d 1 chain
35-70
LENARILKNCVDAKMTEEDKENALSLLDKIYTSPLC
130


1 Pep 2


 


Fel d 1 chain
 1-34
VKMAITCPIFYDVFFAVANGNELLLDLSLTKVNAC
131


2 Pep 1


 


Fel d 1 chain
35-63
TEPERTAMKKIQDCYVENGLISRVLDGLVC
132


2 Pep 2


 


Fel d 1 chain
64-92
CMTTISSSKDCMGEAVQNTVEDLKLNTLGR
133


2 Pep 3


 


Phl p 5 Pep 1
 98-128
CGAASNKAFAEGLSGEPKGAAESSSKAALTSK
134


 


Phl p 5 Pep 2
26-58
ADLGYGPATPAAPAAGYTPATPAAPAEAAPAGKC
135


 


Phl p 5 Pep 3
132-162
AYKLAYKTAEGATPEAKYDAYVATLSEALRIC
136


 


Phl p 5 Pep 4
217-246
CEAAFNDAIKASTGGAYESYKFIPALEAAVK
137


 


Phl p 5 Pep 5
252-283
TVATAPEVKYTVFETALKKAITAMSEAQKAAKC
138


 


Phl p 5 Pep 6
176-212
CAEEVKVIPAGELQVIEKVDAAFK-
139




VAATAANAAPANDK


 


Phl p 5 Pep 1a
 93-128
CFVATFGAASNKAFAEGLSGEPKGAAESSSKAALTSK
141


 


Phl p 5 Pep 2b
26-53
ADLGYGPATPAAPAAGYTPATPAAPAEAC
142









The terms “fragments thereof” and “sequence variations thereof” refer to peptides which are deduced from the allergen-derived molecules disclosed herein and show biochemical properties (e.g. the capacity to prevent IgE binding to the allergen from which those molecules are derived from) which are comparable or identical to said allergen-derived molecules. The fragments of the present invention comprise at least 5, preferably at least 7, more preferably at least 10, successive and/or a maximum of 95%, preferably a maximum of 90%, more preferably a maximum of 80% amino acid residues of the allergen-derived molecule. The term “sequence variation” includes modifications of the peptides such as fragmentation (see above), amino acid substitutions (e.g. with other natural or non-natural amino acids or amino acid derivatives), deletions or additions. “Sequence variation” refers also to said allergen-derived molecules of the above table, wherein at least 1, preferably at least 2, more preferably at least 3, even more preferably at least 4 (5, 6, 7, 8, 9, 10, 15, 20) amino acid residues are added to the C- and/or N-terminus.
It is noted that the clone 30 allergen is an allergen derived from the house dust mite Dermatophagoides pteronyssinus and consists of the following sequence: MANDNDDDPTTTVHPTTTEQPDDKFECPSRFGYFADPKDPHKFYICSNWEAVHKDCPGNTRWNEDEETCT (SEQ ID No. 140; see also AT A 733/2006).
According to the present invention also peptides are encompassed which are at least 80% identical, preferably 90% identical, to the amino sequences disclosed above.
Another aspect of the present invention relates to a nucleic acid molecule encoding a fused hypoallergenic protein according to the present invention.
Another aspect of the present invention relates to a vector comprising a nucleic acid molecule according to the present invention.
Said vector is preferably an expression vector.
The vector harbouring the nucleic acid molecule of the present invention may be used for cloning purposes or for the production of expression vectors. Said vector can be a plasmid, cosmid, virus, bacteriophage or any other vector commonly used in genetic engineering, and can include, in addition to the nucleic acid molecule of the invention, eukaryotic or prokaryotic elements for the control of the expression, such as regulatory sequences for the initiation and the termination of the transcription and/or translation, enhancers, promoters, signal sequences and the like.
According to a preferred embodiment of the present invention the vector is a bacterial, fungal, insect, viral or mammalian vector.
The vector of the present invention may preferably be employed for cloning and expression purposes in various hosts. Therefore, said vector comprises besides a nucleic acid encoding for a hypoallergenic molecule or fusion protein according to the present invention host specific regulatory sequences.
Another aspect of the present invention relates to a host comprising a nucleic acid molecule or a vector according to the present invention.
The nucleic acid molecule and the vector according to the present invention may be introduced into a suitable host. Said molecule may be incorporated into the genome of the host. The vector may exist extrachromosomally in the cytoplasm or incorporated into the chromosome of the host.
Yet another aspect of the present invention relates to an antibody directed against a hypoallergenic molecule, hypoallergenic fusion protein or a fusion protein according to the present invention.
According to a preferred embodiment of the present invention the antibody is a monoclonal or polyclonal antibody.
Antibodies according to the present invention include, but are not limited to, polyclonal, monoclonal, multispecific, humanized or chimeric antibodies, single chain antibodies, Fab fragments, F(ab′) fragments and epitope-binding fragments of any of the above. Furthermore, antibodies are considered to be immunoglobulin molecules and immunologically active portions of immunoglobulin molecules, i.e., molecules that contain an antigen binding site that immunospecifically binds an antigen. The immunoglobulin molecules of the invention are preferably of the types IgG, IgM, IgD, IgA and IgY, class (e.g., IgG1, IgG2, IgG3, IgG4, IgA1 and IgA2) or subclass of immunoglobulin molecule.
Polyclonal antibodies can be prepared by administering a polypeptide of the invention, preferably using an adjuvant, to a non-human mammal and collecting the resultant antiserum. Improved titers can be obtained by repeated injections over a period of time. There is no particular limitation to the species of mammals which may be used for eliciting antibodies; it is generally preferred to use rabbits or guinea pigs, but horses, cats, dogs, goats, pigs, rats, cows, sheep, camels etc., can also be used. In the production of antibodies, a definite amount of immunogen of the invention is, e.g., diluted with physiological saline solution to a suitable concentration, and the resulting diluted solution is mixed with, e.g., complete Freund's adjuvant to prepare a suspension or with mineral gels such as aluminum hydroxide, surface active substances such as lysolecithin, pluronic polyols, polyanions, peptides, oil emulsions, keyhole limpet hemocyanins, dinitrophenol, and potentially useful human adjuvants such as BCG (bacille Calmette-Guerin) and corynebacterium parvum. The suspensions and mixtures are administered to mammals, e.g., intraperitoneally, e.g., to a rabbit, using from about 50 μg to about 2,500 μg polypeptide of the invention per administration. The suspension is preferably administered about every two weeks over a period of up to about 2-3 months, preferably about 1 month, to effect immunization. The antibody is recovered by collecting blood from the immunized animal after the passage of 1 to 2 weeks after the last administration, centrifuging the blood and isolating serum from the blood.
Monoclonal antibodies may, e.g., be of human or murine origin. Murine monoclonal antibodies may be prepared by the method of Köhler and Milstein (Köhler, G. and Milstein, C., Nature 256 (1975) 495), e.g., by fusion of spleen cells of hyperimmunized mice with an appropriate mouse myeloma cell line.
A chimeric antibody is a molecule in which different portions of the antibody are derived from different animal species, such as antibodies having a variable region derived from a murine monoclonal antibody and a human immunoglobulin constant region. Methods for producing chimeric antibodies are known in the art. See, e.g., Morrison, Science 229:1202 (1985); Oi et al., BioTechniques 4:214 (1986); Gillies et al., (1989) J. Immunol. Methods 125:191-202; U.S. Pat. No. 5,807,715; U.S. Pat. No. 4,816,567 and U.S. Pat. No. 4,816,397.
Humanized antibodies are antibody molecules from a non-human species antibody that binds the desired antigen having one or more complementarity determining regions (CDRs) from the non-human species and framework regions from a human immunoglobulin molecule. Often, framework residues in the human framework regions will be substituted with the corresponding residue from the CDR donor antibody to alter, preferably improve, antigen binding. These framework substitutions are identified by methods well known in the art, e.g., by modelling of the interactions of the CDR and framework residues to identify framework residues important for antigen binding and sequence comparison to identify unusual framework residues at particular positions (see, e.g., Queen et al., U.S. Pat. No. 5,585,089; Riechmann et al., Nature 332:323 (1988)). Antibodies can be humanized using a variety of techniques known in the art including, for example, CDR-grafting (EP 239,400; WO 91/09967; U.S. Pat. No. 5,225,539; U.S. Pat. No. 5,530,101; and U.S. Pat. No. 5,585,089), veneering or resurfacing (EP 592,106; EP 519,596; Padlan, Molecular Immunology 28(4/5):489-498 (1991); Studnicka et al., Protein Engineering 7(6):805-814 (1994); Roguska. et al., PNAS 91:969-913 (1994)), and chain shuffling (U.S. Pat. No. 5,565,332).
The antibodies according to the present invention may advantageously be used for desensitization of an individual suffering from an allergy, in particular from house dust mite allergy. For passive immunisation the antibody is preferably an IgG or a derivative thereof (e.g., chimeric or humanized antibody). Furthermore, this antibody may also be used for desensibilisation of an individual.
Another aspect of the present invention relates to a vaccine formulation comprising a hypoallergenic protein or an antibody according to the present invention.
The vaccine formulation according to the present invention may be formulated as known in the art and necessarily adapted to the way of administration of said vaccine formulation.
Preferred ways of administration of the vaccine formulation (of the present invention) include all standard administration regimes described and suggested for vaccination in general and allergy immunotherapy specifically (orally, transdermally, intraveneously, intranasally, via mucosa, rectally, etc). However, it is particularly preferred to administer the molecules and proteins according to the present invention subcutaneously or intramusculary.
The vaccine formulation according to the present invention may only comprise a viral capsid protein or fragments thereof of a member of the genus of rhinovirus
Said formulation preferably further comprises at least one adjuvant, pharmaceutical acceptable excipient and/or preservative.
In order to increase the immunogenicity of the hypoallergenic molecules according to the present invention, adjuvants, for instance, may be used in a medicament according to the present invention. An adjuvant according to the present invention is an auxiliary agent which, when administered together or in parallel with an antigen, increases its immunogenicity and/or influences the quality of the immune response. Hence, the adjuvant can, e.g., considerably influence the extent of the humoral or cellular immune response. Customary adjuvants are, e.g., aluminum compounds, lipid-containing compounds or inactivated mycobacteria.
Generally, adjuvants can be of different forms, provided that they are suitable for administration to human beings. Further examples of such adjuvants are oil emulsions of mineral or vegetal origin, mineral compounds such as aluminium phosphate or hydroxide, or calcium phosphate, bacterial products and derivatives, such as P40 (derived from the cell wall of Corynebacterium granulosum), monophosphoryl lipid A (MPL, derivative of LPS) and muramyl peptide derivatives and conjugates thereof (derivatives from mycobacterium components), alum, incomplete Freund's adjuvant, liposyn, saponin, squalene, etc. (see, e.g., Gupta R. K. et al. (Vaccine 11:293-306 (1993)) and Johnson A. G. (Clin. Microbiol. Rev. 7:277-289).
According to another preferred embodiment of the present invention said formulation comprises 10 ng to 1 g, preferably 100 ng to 10 mg, especially 0.5 μg to 200 μg of said hypoallergenic molecule or antibody.
Another aspect of the present invention relates to the use of a hypoallergenic protein or an antibody according to the present invention for manufacturing a medicament for the treatment or prevention of a viral infection and/or an allergy in a human or animal.
Said medicament preferably further comprises at least one adjuvant, pharmaceutical acceptable excipient and/or preservative.
The medicament according to the present invention may be used for active (administration of the hypoallergenic protein and/or molecules of the invention) as well as for passive immunization (antibodies directed to the hypoallergenic protein and/or molecules of the invention).
According to a preferred embodiment of the present invention said medicament comprises 10 ng to 1 g, preferably 100 ng to 10 mg, especially 0.5 μg to 200 μg of said hypoallergenic molecule, nucleic acid molecule, vector, host or antibody.
The medicament is preferably administered to an individual in amount of 0.01 mg/kg body weight to 5 mg/kg body weight, preferably 0.1 mg/kg body weight to 2 mg/kg body weight.
The particular dosage regimen, i.e., dose, timing and repetition, will depend on the particular individual and that individual's medical history. Empirical considerations, such as the half life, will generally contribute to determination of the dosage. Frequency of administration may be determined and adjusted over the course of therapy.
The individual to whom the medicament according to the present invention is administered is preferably an individual or animal which is at risk to become an allergy.
Subjects having or at risk of having an allergic condition, disorder or disease include subjects with an existing allergic condition or a known or a suspected predisposition towards developing a symptom associated with or caused by an allergic condition. Thus, the subject can have an active chronic allergic condition, disorder or disease, an acute allergic episode, or a latent allergic condition, disorder or disease. Certain allergic conditions are associated with seasonal or geographical environmental factors. Thus, at risk subjects include those at risk from suffering from a condition based upon a prior personal or family history, and the season or physical location, but which the condition or a symptom associated with the condition may not presently manifest itself in the subject.
The administration of the medicament according to the present invention, which comprises at least one hypoallergenic molecule as described herein, to an individual may prevent sensitization of said individual or may induce an appropriate immune response to allergens. If the medicament of the present invention is used to prevent sensitization, it should be administered to an individual prior to the first contact with said allergen. Therefore, it is preferred to administer the medicament according to the present invention to neonates and children. It turned out that also the administration of the medicament according to the present invention to pregnant individuals will induce the formation of antibodies directed against allergens in the unborn child. It is especially beneficiary to use hypoallergenic molecules according to the present invention for such therapies, because due to the lack of T-cell epitopes side effects occurring in the course of allergen immunotherapy can significantly be reduced or even be completely avoided.
Yet another aspect of the present invention relates to the use of a hypoallergenic protein or an antibody according to the present invention for the diagnosis of an allergy and/or a viral infection in an individual.
Another aspect of the present invention relates to the use a viral capsid protein from a virus of the family of picornaviridae as a carrier in medicaments or vaccines or for diagnosing a viral infection, in particular common cold.
As a valuable alternative to the widely spread KLH carrier protein viral capsid proteins of viruses of the family of picornaviridae may be used. The carrier may be conjugated chemically or fused with recombinant techniques to peptides, proteins and polypeptides or other antigens. Furthermore, the viral capsid protein may be used to detect, e.g., antibodies directed to said capsid protein in the serum of an individual.
One of the advantages of such a carrier is that not only the antigen fused or conjugated thereon may be exposed to the immune system, but also an immune response against the capsid protein of a rhinovirus is induced. Consequently, such a vaccination leads to the prevention and/or treatment of diseases caused by rhinoviruses. The virus is preferably of the species of human rhinoviruses, in particular human rhinovirus 89 and 14.
Another aspect of the present invention relates to a hypoallergenic molecule derived from Phl p 5 (Genbank Nr. X7435) having a C- and/or N-terminal truncation and lacking substantially IgE-binding capacity.
Grass pollen is one of most potent outdoor seasonal sources of airborne allergens responsible for hay fever and allergic asthma.
More than 40% of allergic individuals display IgE-reactivity with grass pollen allergens, which are divided into more than 11 groups. More than 80% of the grass pollen allergic patients react with group 5 allergens.
Group 5 allergens are non-glycosylated, highly homologous proteins with a molecular mass range from 25-33 kD. Several group 5 allergens have been cloned and/or immunologically characterized.
The trial to reduce the allergenic activity by introducing pointmutations, mutations of several amino acids in row or deletions showed no effect (Schramm G, et al. J Immunol 1999; 162: 2406-1435). IgE-binding regions of Phl p 5 (Flicker S, et al. J Immunol 2000; 165: 3849-3859) have already been described and the three-dimensional structure has been solved (Maglio O, et al. 2002. Protein Eng. 15:635-642).
It turned out that in particular the Phl p 5 peptides according to the present invention, which are C- and/or N-terminally truncated and lack IgE-binding capacity, may be employed for the active vaccination of individuals.
According to a preferred embodiment of the present invention the truncated molecule lacks T-cell epitopes.
As already outlined above, late side effects of allergen immunotherapy can be significantly reduced or even be avoided if the hypoallergenic molecules substantially lack T-cell epitopes.
Truncated Phi p 5 molecules lacking T-cell epitopes are composed of amino acids 93 to 128, 98 to 128, 26 to 53, 26 to 58 or 252 to 283 of Phl p 5 or fragments or sequence variations thereof.
In particular these truncated molecules substantially show no T-cell epitopes and are, nevertheless, able to provoke an appropriate immune response directed against the wild-type allergen.
According to another preferred embodiment of the present invention the hypoallergenic truncated Phl p 5 is composed of amino acids 132 to 162, 217 to 246 or 176 to 212 of Phl p 5 or sequence variations thereof.
These hypoallergenic molecules comprise one or more T-cell epitopes but lack IgE-binding capacity.
Another aspect of the present invention relates to a hypoallergenic molecule derived from Fel d 1 (Genbank Nr. X62477 and X62478) having a C- and/or N-terminal truncation and lacking IgE-binding capacity.
Allergies to animals affect up to 40% of allergic patients. In the domestic environment, allergies to the most popular pets, cats and dogs, are particularly prevalent and connected with perennial symptoms. Animal allergens are present in dander, epithelium, saliva, serum or urine. Exposure to the allergens can occur either by direct skin contact or by inhalation of particles carrying the allergens. The major cat and dog allergens were shown to be present widespread and could even be detected in non-pet owning households and in public places, e.g., schools. This can be attributed to the high and increasing number of households keeping pets in industrialized countries (about 50%) and the high stability of the allergens, which are carried off and distributed.
Fel d 1 was identified as the major cat allergen, which is recognized by more than 90% of cat allergic patients. Fel d 1 represents a 38 kDa acidic glycoprotein of unknown biological function. It consists of two identical non-covalently linked heterodimers, which, again, are composed of two polypeptide chains antiparallely linked by three disulfide bonds. Chain 1 and chain 2 are encoded on different genes, each consisting of 3 exons. Recombinant Fel d 1 (rFel d 1), expressed as a chain 2- to chain 1 fusion protein, has been generated in E. coli. This recombinant Fel d 1 is able to completely mimic the immunological properties of the wild-type allergen.
Peptides derived from the major cat allergen Fel d 1, and lacking IgE-binding capacity are suitable, e.g., for immunotherapy and prophylactic allergy vaccination. These peptides may be comprised in a larger polypeptide or be coupled to a suitable carrier protein such as keyhole limpet hemocyanin (KLH). The Fel d 1-derived synthetic peptides—like the Phl p 5 and allergen-derived peptides disclosed herein—are capable of inducing an IgG response, i.e., the production of so called “blocking antibodies” or “protective antibodies”. These antibodies prevent IgE-binding to the allergen Fel d 1. A significant reduction in allergic symptoms may thus be achieved.
According to a preferred embodiment of the present invention the truncated molecule exhibits reduced T-cell reactivity.
In order to avoid or to significantly reduce late side effects the Fel d 1 derived hypoallergenic molecule exhibits reduced T-cell reactivity as defined in the present invention.
The truncated Fel d 1 is preferably composed of amino acids 1 to 34 or 35 to 70 of chain 1 of Fel d 1, amino acids 1 to 34, 35 to 63 or 64 to 92 of chain 2 of Fel d 1 or sequence variations thereof.
Another aspect of the present invention relates to hypoallergenic molecules being composed of or comprising amino acids 1 to 33, 21 to 51, 42 to 73, 62 to 103 or 98 to 129 of Der p 2, amino acids 1 to 30, 20 to 50, 50 to 80, 90 to 125, 125 to 155 or 165 to 198 of Der p 7, amino acids 1 to 35, 35 to 72, 70 to 100 or 90 to 122 of Der p 21, amino acids 1 to 32, 15 to 48 or 32 to 70 of Clone 30, amino acids 19 to 58, 59 to 95, 91 to 120 or 121 to 157 of Alt a 1, amino acids 31 to 60, 45 to 80, 60 to 96 or 97 to 133 of Par j 2, amino acids 1 to 40, 36 to 66, 63 to 99, 86 to 120 or 107 to 145 of Ole e 1, amino acids 25 to 58, 99 to 133, 154 to 183, 277 to 307, 334 to 363, 373 to 402, 544 to 573, 579 to 608, 58 to 99, 125 to 165, 183 to 224, 224 to 261, 252 to 289, 303 to 340, 416 to 457, 460 to 500 or 501 to 542 of Fel d 2, amino acids 19 to 58, 52 to 91, 82 to 119, 106 to 144 or 139 to 180 of Can f 2, amino acids 19 to 56, 51 to 90, 78 to 118, 106 to 145 or 135-174 of Can f 1, amino acids 27 to 70, 70 to 100 or 92 to 132 of Art v 1, amino acids 31 to 70, 80 to 120, 125 to 155, 160 to 200, 225 to 263, 264 to 300 305 to 350 or 356 to 396 of Amb a 1, amino acids 1 to 34, 35 to 74, 74 to 115, 125 to 165, 174 to 213, 241 to 280, 294 to 333, 361 to 400 or 401 to 438 of Alt a 6, amino acids 1 to 40, 41 to 80, 81 to 120, 121 to 160 of Alt a 2 or fragments or sequence variations thereof.
Another aspect of the present invention relates to a hypoallergenic fusion protein comprising at least two hypoallergenic molecules according to the present invention exhibiting reduced IgE-binding capacity and exhibiting optionally reduced T-cell reactivity.
The hypoallergenic molecules of the present invention which are derived from an allergen and lack IgE-binding capacity may be fused recombinantly or conjugated chemically to each other. As single components (allergen fragments) of the fusion protein/polypeptide also said fusion protein/polypeptide lacks IgE-binding capacity.
The fusion protein according to the present invention may comprise at least two, preferably at least three, more preferably at least four, even more preferably at least five, hypoallergenic molecules according to the present invention. It is, of course, also possible to fuse the hypoallergenic molecules to other peptides, polypeptides and proteins not derived from allergens. These peptides, polypeptides and proteins may when administered to an individual also induce an immunologic reaction or may act as a carrier or exhibit enzymatic activities. The hypoallergenic molecules in the fusion protein according to the present invention may be coupled directly to each other or via a linker which is preferably composed of amino acid residues.
Methods for the production of fusion proteins are well known in the art and can be found in standard molecular biology references such as Sambrook et al. (Molecular Cloning, 2nd ed., Cold Spring Harbor Laboratory Press, 1989) and Ausubel et al. (Short Protocols in Molecular Biology, 3rd ed; Wiley and Sons, 1995). In general, a fusion protein is produced by first constructing a fusion gene which is inserted into a suitable expression vector, which is, in turn, used to transfect a suitable hosT-cell. In general, recombinant fusion constructs are produced by a series of restriction enzyme digestions and ligation reactions which result in the desired sequences being incorporated into a plasmid. If suitable restriction sites are not available, synthetic oligonucleotide adapters or linkers can be used as is known by those skilled in the art and described in the references cited above. The polynucleotide sequences encoding allergens and native proteins can be assembled prior to insertion into a suitable vector or the sequence encoding the allergen can be inserted adjacent to a sequence encoding a native sequence already present in a vector. Insertion of the sequence within the vector should be in frame so that the sequence can be transcribed into a protein. It will be apparent to those of ordinary skill in the art that the precise restriction enzymes, linkers and/or adaptors required as well as the precise reaction conditions will vary with the sequences and cloning vectors used. The assembly of DNA constructs, however, is routine in the art and can be readily accomplished by a person skilled in the art.
According to a preferred embodiment of the present invention the molecules are fused to each other in an order differing from the order of the fragments in the wild-type allergen if the at least two molecules are derived from the same allergen.
The fusion protein according to the present invention may comprise at least two hypoallergenic molecules which are derived from the same wild-type allergen. In such a case the single molecules (allergen fragments) are fused to each other in an order differing from the order in the wild-type allergen. Such an approach prevents the re-formation of potential IgE-binding sites/epitopes in the hypoallergenic fusion protein.
Another aspect of the present invention relates to a nucleic acid molecule coding for a hypoallergenic molecule and a fusion protein according to the present invention.
The nucleic acid molecule of the present invention may be employed, e.g., for producing said molecules recombinantly.
Said nucleic acid molecule may—according to another aspect of the present invention—be comprised in a vector.
This vector is preferably an expression vector.
Another aspect of the present invention relates to a vaccine formulation comprising a hypoallergenic molecule, a fusion protein or an antibody according to the present invention.
The formulation further comprises preferably at least one adjuvant, pharmaceutical acceptable excipient and/or preservative.
The use of particular carrier substances such as KLH (keyhole Limpet Hemocyanin) is also among the latest current methods to increase immune responses. The hypoallergenic molecules of the present invention may also be fused or conjugated to viral capsid proteins which may act also as a carrier (see above).
According to a preferred embodiment of the present invention said formulation comprises 10 ng to 1 g, preferably 100 ng to 10 mg, especially 0.5 μg to 200 μg of said hypoallergenic molecule or antibody.
The vaccine formulation may be substantially composed as the medicament of the present invention (see above).
Another aspect of the present invention relates to the use of a hypoallergenic molecule, a fusion protein, or an antibody according to the present invention for manufacturing a medicament for the treatment or prevention of an allergy in an individual.
The hypoallergenic molecule, fusion protein and antibody according to the present invention may be used for vaccination of an individual. This vaccination may reduce or prevent the allergic response caused by a wild-type allergen.
According to a preferred embodiment of the present invention said medicament further comprises at least one adjuvant, pharmaceutical acceptable excipient and/or preservative.
The medicament according to the present invention comprises preferably 10 ng to 1 g, preferably 100 ng to 10 mg, especially 0.5 μg to 200 μg of said immunogenic molecule, nucleic acid molecule, vector, host or antibody.
According to another preferred embodiment of the present invention the medicament is administered to an individual in the amount of 0.01 mg/kg body weight to 5 mg/kg body weight, preferably 0.1 mg/kg body weight to 2 mg/kg body weight.
According to a preferred embodiment of the present invention said individual is at risk to get an allergy.
Another aspect of the present invention relates to the use of a hypoallergenic molecule, a fusion protein or an antibody according to the present invention for diagnosing an allergy or monitoring the progress of an allergy therapy in an individual.
The hypoallergenic molecule, fusion protein or antibody according to the present invention may not only be used in medicaments but can also be suitably employed for various diagnostic purposes. For instance, these molecules and fusion proteins may be used for diagnosing an allergy by exposing, e.g., a sample of an individual comprising histamine releasing cells to said polypeptide (see, e.g., Purohit et al., Clin. Exp. Allergy 35 (2005): 186-192). Furthermore, these molecules, fusion proteins and antibodies may be immobilized on a surface in order to form a polypeptide array/chip. Such arrays may be used, e.g., in high throughput screening in order to diagnose an allergy in a number of samples taken from a number of individuals.



The present invention is further illustrated by the following figures and examples, however, without being restricted thereto.
FIG. 1A shows a schematic overview of the vector p89VP1.
FIGS. 1B and 1C show the DNA sequence of the multiple cloning site of the pET-17b vector and the 89VP1 encoding gene.
FIG. 1D shows the schematic representation of three possibilities for creating nucleic acid fusions.
FIG. 2 shows a Coomassie blue stained 12% SDS-PAGE gel containing purified 89VP1 his-tagged protein (Lane 1: 5 μg molecular marker; Lane 2-5: 10 μl 89VP1 elution samples).
FIG. 3 shows IgG recognition of 14VP1: Immunoblotting of 14VP1 and controls. Dots are visualized by autoradiography (Lane 1-6: Incubation with 1:500-1:16000 diluted rabbit anti-14VP1 antiserum; Lane 7-12: Incubation with 1:500-1:16000 diluted preimmune serum).
FIG. 4 shows 89VP1-specific IgG1 response in mice. Groups of mice were immunized with different antigens as indicated on top of each box. 89VP1-specific IgG1 titers were measured by ELISA and are expressed as OD values on the y-axis. The results are shown as box plots, where 50% of the values are within the boxes and non-outliers between the bars. The line within the boxes indicates the median values.
FIG. 5 shows Phl p 1-specific IgG1 response in mice. Groups of mice were immunized with different antigens as indicated on top of each box. rPhl p 1-specific IgG1 titers were measured by ELISA and are expressed as optical value (OD 405 nm) on the y-axis. The optical value corresponds to the level of IgG1 antibody in the mouse sera. The results are shown as box plots were 50% of the values are within the boxes and non-outliers between the bars. The line within the boxes indicates the median values.
FIG. 6 shows Timothy grass pollen extract-specific IgG1 response in immunized mice. Groups of mice were immunized with different antigens as indicated on top of each box. Timothy grass pollen extract-specific IgG1 titers were measured by ELISA and are expressed as optical value (OD 405 nm) on the y-axis. The optical value corresponds to the level of IgG1 antibody in the mouse sera. The results are shown as box plots, where 50% of the values are within the boxes and non-outliers between the bars. The line within the boxes indicates the median values.
FIG. 7 shows the mean of % inhibition of patient's IgE-binding to rPhl p 1 by preincubation with antisera against rPhl p 1, r89P5 and KLHP5 of all 19 patients. The % inhibition is shown on the y-axis. The results are shown as bars.
FIG. 8 shows the proliferation of spleen cells of immunized mice. T-cells of immunized mice with different antigens as indicated on top of each box were stimulated with peptide 5, 89VP1(89) and KLH. Medium was used as a reference. At the y-axis the stimulation index is shown. The results are displayed in bars.
FIG. 9 shows IgG1, IgG2, IgG4 and IgA response to 14VP1, 89VP1 and rPhl p 1 detected in human sera by ELISA measurement. 10 patient's sera were tested for four antibodies specific for 89VP1, 14VP1 and rPhl p 1 as indicated on top of each box. Sera taken in autumn and winter are shown on the left hand and right hand of each “bar pair”, respectively. Titers were measured by ELISA and are expressed as optical value (OD 405 nm) on the y-axis. The optical value corresponds to the level of antibody in the human sera. The results are shown as box plots, where 50% of the values are within the boxes and non-outliers between the bars. The line within the boxes indicates the median values.
FIG. 10 shows the detection of anti-89VP1 and anti-rPhl p 1 antibodies in sera of allergic patients. 5 Phl p 1 allergic patient's sera were tested for seven antibodies specific for 89VP1 (left bar of each pair) and rPhl p 1 (right bar of each pair). Titers were measured by ELISA and are expressed as optical value (OD 405 nm) on the y-axis. The optical value corresponds to the level of antibody in the human sera. The results are shown as box plots, where 50% of the values are within the boxes and non-outliers between the bars. The line within the boxes indicates the median values.
FIG. 11 shows anti-14VP1 IgG binding to HRV14 protein extract and purified 14VP1 (Lane 1 and 4: 5 μg Marker; lane 2 and 4: Virus extract; lane 2 and 5: 5 μg 14VP1). Blot A und B was incubated with anti-14VP1 immune serum and preimmune serum, respectively. Bound IgG was detected by Western blotting and visualized by autoradiography.
FIG. 12 shows the neutralization of HRV14 (Lane A (cell control): Cells after preincubation of HRV14 with a dilution of anti-14VP1 immune serum 1:102-1:108 (row A1-A6); Lane B: Cells after preincubation of HRV14 with a dilution of preimmune serum 1:102-1:108 (row B1-B6); Lane C: cells after Incubation with HRV14 10 TCD50-106 TCD50 (row C1-C6); D5: cells after incubation with preimmune serum; D6: cells after incubation with immune serum). Cells were plated out in all wells and colored with crystal violet after three days.
FIG. 13 shows IgG reactivity of anti-peptide antisera with the complete Phl p 5 allergen. IgG reactivities (OD values) to Phl p 5 are shown for 3 serum samples (bleeding 1: preimmune serum; bleedings 2-3: serum samples collected in monthly intervals) obtained from 6 rabbits, each of which was immunized with one of the KLH-conjugated peptides.
FIG. 14 shows allergenic activity of rPhl p 5, and the peptide mix as detected by CD203c expression. Heparinized blood samples from three patients allergic to Phl p 5 were incubated with serial dilutions from 10−4 to 10 μg/mL of recombinant allergen, an equimolar mix of Phl p 5 derived peptides, anti-IgE or control buffer (co, x-axis). Cells were then stained with CD203c mAb and analyzed for CD203c expression on a FACScan. The stimulation index (SI) is displayed on the y-axis.
FIG. 15 shows identification of Phl p 5-derived peptides which induce low lymphoproliferative responses. PBMCs from timothy pollen allergic patients were stimulated with different concentrations of peptides and, for control purposes, with interleukin-2 (x-axis). Stimulation indices (SI) are indicated on the y-axis.
FIG. 16 shows Fel d 1-derived synthetic peptides which induce Fel d 1 specific IgG immune responses in rabbits. Six rabbits were immunized with the KLH-conjugated Fel d 1-derived synthetic peptides or unconjugated rFel d 1 and 3-4 bleedings were drawn in monthly intervals. IgG reactivities of the preimmune sera and the antisera to ELISA plate-bound rFel d 1 are shown as optical densities (O.D. values, y-axis).
FIG. 17 shows the low allergenic activity of Fel d 1-derived synthetic peptides as determined by CD63 and CD203c expression on basophils of allergic patients. PBMCs from 5 cat allergic patients were incubated with serial dilutions of Fel d 1 (closed boxes) or a mixture of Fel d 1-derived synthetic peptides (open boxes) (x-axis). For patient RR PBMCs were also probed with serial dilutions of Fel d 1-derived synthetic peptides as single components. Induction of expression of surface markers CD203c and CD63 was measured as mean fluorescence intensities, and calculated stimulation indices are shown on the y-axis.
FIG. 18 shows that a treatment with KLH-coupled Bet v 1-derived peptides reduces lymphoproliferative responses to rBet v 1 in sensitized mice. T-cell proliferation was measured in spleen cell cultures after in vitro stimulation with the recombinant birch pollen allergen Bet v 1 (white bars), KLH (black bars), or the peptide mix (grey bars). The bars represent the mean stimulation indices (SI±SD) for the different groups of mice.
FIG. 19 shows the prophylactic vaccination of naive mice with KLH-coupled Bet v 1-derived peptides which reduces lymphoproliferative responses to rBet v 1 after sensitization.
FIG. 20 shows that the prophylactic vaccination of naive mice with KLH-coupled Bet v 1-derived peptides induces a Bet v 1-specific IgG response and primes for the induction of allergen-specific IgG responses by the complete allergen. IgG responses (OD values: y-axis) to Bet v 1 were measured in the four treatment groups at different points of time (x-axis).
FIG. 21 shows a comparison of IgE-reactivity: IgE binding capacity of Phl p 5 derived peptides (1, 2) and variants (1a, 2b) was determined in dot blot assays applying 0.2 □g/dot using sera from 7 grass-pollen allergic patients (p1-p7) and the serum from a non-atopic individual (NHS). rPhl p 5 was used as positive control and HSA as negative control. Bound IgE was detected with 125 I-labelled anti-human IgE.
FIG. 22 shows a lymphoproliferative responses of Phl p 5 derived peptides (1, 2) and variants (1a, 2b). PBMCs from grass pollen allergic patients were stimulated with different concentrations of peptides and, for control purposes, with equimolar concentrations of rPhl p 5. Stimulation indices (SI) are indicated on the y-axis.
FIG. 23 shows the cross protection of anti VP1 antibodies.



EXAMPLES
Example 1
Construction of Vector p89VP1
Virus stock samples were prepared for RT-PCR by addition of 1 μl of RNase inhibitor (Boehringer GmbH, Germany) to a final concentration of 0.01 U/μl after RNA extraction from cell culture supernatants by QIAamp viral RNA kit (Qiagen, Germany).
Plasmid p89VP1 (FIG. 1A) was constructed by replacing the NdeI/EcoRI fragment of the multiple cloning site of pET-17b with the cDNA sequence encoding for the VP1 protein of human rhinovirus strain 89 (89VP1). The NdeI and EcoRI restriction sites (in pET-17b) were used for insertion of AseI/EcoRI linked 89VP1 with ATG at the 5′ end, six histidin followed by the stop-codon TGA at the 3′ end (FIG. 1B).
The insertion of 89VP1 in pET-17b was confirmed by nucleotide sequencing.
After NdeI/AseI fusion instead of the NdeI site CATAAT was created and could not be cut with any available enzyme. Therefore, the site was mutated to CTTAAG, the restriction site of Afl II. To insert a further allergen fragment, the ACCGTT sequence at the 3′ end was mutated to ACCGGT, the restriction site of AgeI. The amino acid sequences are displayed below the nucleotide sequences of 89VP1. The restriction sites are shown underlined in FIG. 1B.
Said Afl II and AgeI restriction sites were created with the Quick change site mutagenesis Kit (Stratagene).
cDNAs for gene fragments can thus be inserted for easy purification into the remaining restrictions sites either at the 5′ end (using Afl II) or at the 3′ end (using AgeI) of 89VP1 or into both sites as indicated in FIG. 1C. Recombinant allergen fragments will thus be expressed at the N-terminus and/or the C-terminus of the 89VP1.







TABLE I







Cloning and mutagenesis primers (5′ to 3′)











SEQ




ID


89VP1 cloning

No.





89VP1forward
CGGAATTCATTAATATGAACCCAGTTGAAAAT-
1



TATATAGATAGTGTATTA


 


89VP1 reverse
CGATTAATTCAGTGGTGGTGGTGGTGGTG-
2



GACGTTTGTAACGGTAA


 


Mutagenesis


Afl II
CTTTAAGAAGGAGATATACTTAAGATGAAC-
3


forward
CCAGTTG


 


Afl II
CAACTGGGTTCATCTTAAGTATATCTCCTTCT-
4


reverse
TAAAG


 


AgeI forward
CCTGATGTTTTTACCGGTACAAACGTCCACCAC
5


 


AgeI reverse
GTGGTGGACGTTTGTACCGGTAAAAACATCAGG
6









Example 2
Cloning of a Construct Expressing a 89VP1-Allergen Fragment Fusion Protein
The approach described above was exemplified for a C-terminal Phl p 1 allergen fragment, namely peptide 5 (CVRYTTEGGTKTEAEDVIPEGWKADTAYESK; M. Focke et al. FASEB J (2001) 15:2042-4). The peptide 5 DNA sequence was amplified by PCR using the Phl p 1 cDNA (GenBank: X78813) as a template (Primers: Phl p 1 forward 5′-CGCGCTTAAGATGGTCCGCTACACCACCGAGGGC-3′ (SEQ ID No. 7); Phl p 1 reverse 5′-CGCGCTTAAGCTTGGACTCGTAGGCGGTGTCGGC-3′ (SEQ ID No. 8)). The PCR product was inserted into the Afl II restriction site of the p89VP1 vector, and the resulting construct is referred to as vector p89P5 and gene product r89P5.
Example 3
Expression and Purification of 89VP1 Peptide 5-Fusion Protein and 89VP1
In order to achieve expression of 89VP1 or r89P5 (recombinant 89VP1-peptide 5 fusion protein), plasmids were transformed into E. coli BL21 (DE3). Transformed E. coli cells were grown in 250 ml LB medium containing 100 mg/l ampicillin at 37° C. to an optical density (600 nm) of 0.4, and protein expression was induced by addition of isopropyl-beta-D-thiogalactosidase (IPTG) to a final concentration of 1 mM. E. coli cells were harvested after 4 hours by centrifugation at 3500 rpm at 4° C. for 10 min. Purification was performed with the Qiagen protocol using Qiagen Ni-NTA and columns. The cell pellet was resuspended under denaturing conditions in 5 ml 6 M guanidinium hydrochloride for 1 hour. After centrifugation (20 min, 10000×g) the supernatant was incubated with 1 ml Ni-NTA for an additional hour. The suspension was then loaded on a column, washed twice with 5 ml wash buffer (8 M urea pH 6.3) and then eluted with 4 ml elution buffer (8 M urea pH 3.5). Renaturation was achieved after dialysis with decreasing molarity of urea.
Purity and size of the purified protein were analyzed by SDS-PAGE as shown in FIG. 2. Protein bands correlated with the calculated protein size of 33.6 kD. Integrity of the proteins was also confirmed by Western blot analysis, using anti-histidine tag antibody.
Example 4
Detection of 14VP1-Specific Rabbit Antibodies by Immunoblotting
5 μg 14VP1 (VP1 protein of the human rhinovirus strain 14) and controls (Bet v 1, Phl p 5, BSA) were dotted onto nitrocellulose membrane strips. These strips were exposed to a dilution of rabbit anti-14VP1 antiserum (lane 1-6) and preimmune serum (lane 8-12). Bound rabbit IgG antibodies were detected with 125I-labeled donkey anti-rabbit IgG 1:1000 and visualized by autoradiography (FIG. 3).
Dot blot analysis of rabbit anti-14 VP1 serum shows that 14VP1 is strongly immunogenic. IgG antibodies were still detected with a 1:16000 dilution of the antiserum in contrast to the preimmune serum and the Bet v 1, Phl p 5 and BSA controls.
Example 5
89VP1-Specific Antibody Response in Immunized Mice Determined by ELISA
In order to determine the immunogenicity of 89VP1 and its ability to act as a carrier for peptide 5, groups of six week old female balb/c mice (Charles River) were immunized with the following antigens: KLH, KLH maleimide coupled peptide 5 (KLHP5) and KLH EDC coupled peptide 5 (KLHP5edc). The chemical coupling was made with the Imject maleimide activated mcKLH and Imject Immunogen EDC Kit (Pierce). The maleimide group reacts with SH groups and the EDC reacts with carboxyl and amino groups to form stable bonds. Groups of 4 mice were immunized with 89VP1, r89P5 and 89P5edc and 2 mice were immunized with peptide 5 only (5 μg each and mixed 1:2 with aluminium hydroxide). The mice were immunized subcutaneously in approximately three-week intervals and bled from the tail veins. The 89VP1-specific IgG1 antibody level was determined by ELISA.
ELISA plates (Nunc) were coated with 5 μg/ml 89VP1. The mice anti-89VP1, anti-r89P5 and peptide 5 sera were diluted 1:500. Bound IgG1 was detected with rat anti-mouse IgG1 (BD Pharmingen) 1:1000 and then with goat anti rat IgG POX-coupled (Amersham Bioscience) 1:2000. The optical value (OD 405 nm) is displayed on the y-axis and corresponds to the level of IgG1 antibody in the mouse sera (FIG. 4).
KLHP5, KLHP5edc, KLH and peptide 5 were used as controls. IgG1 antibodies were detected with an increasing titer during immunization in mice injected with 89VP1 (89VP1, 89P5edc and r89P5). Rabbits immunized with 89VP1, r89P5 and KLHP5 show the same result.
Example 6
rPhl p 1-Specific Antibody Response in Immunized Mice Determined by ELISA
To evaluate whether immunization with r89P5 will induce IgG antibodies that react with complete Phl p 1, the same method and the same mice sera were used as described in example 5. ELISA plates were coated with 5 μg/ml rPhl p 1 and the IgG1 antibody titer was determined (FIG. 5).
All Phl p 1 derived peptides either coupled to KLH or 89VP1 induced rPhl p 1 specific IgG1 antibodies with increasing responses during immunizations. Rabbits immunized with r89P5 and KLHP5 show the same result.
Example 7
ELISA Detection of Timothy Grass Pollen Extract-Specific IgG1 Antibodies
Immunization of mice and ELISA analysis was performed as described in section 5. Whole timothy grass pollen extract was coated (100 μg/ml) on ELISA plates and the IgG1 antibody titer was determined (FIG. 6).
After three immunizations extract-specific IgG1 antibodies could be detected in mice immunized with peptide 5.
Example 8
Rabbit Anti-r89P5 Antibodies Block Patient's IgE-Binding to rPhl p 1
To determine the ability of peptide-induced rabbit Ig to inhibit the binding of allergic patients' IgE antibodies to rPhl p 1, ELISA plates were coated with 1 μg/ml rPhl p 1, washed and blocked. The plates were preincubated with 1:100-diluted rabbit anti-peptide (89P5, KLHP5), a rabbit anti rPhl p 1 and, for control purposes, with the corresponding preimmune sera. After washing, plates were incubated with human sera from Phl p 1-allergic patients (1:3 diluted) and bound IgE was detected with mouse anti-human IgE (Pharmingen 1:1000) and then with sheep anti-mouse IgG POX-coupled (Amersham Bioscience) 1:2000. The percentage of inhibition of IgE-binding achieved by preincubation with the anti peptide antisera was calculated as follows: 100−ODi/ODp×100.
ODi and ODp represent the extinctions after preincubation with the rabbit immune and preimmune serum, respectively. Table 2 shows the capacity of anti-Phl p 1 peptide antibodies to inhibit the binding of 19 allergic patients' IgE to complete rPhl p 1. FIG. 7 displays the mean inhibition (in %) of all anti-rPhl p 1, anti-r89P5 and anti-KLHP5 immune sera. Anti-peptide sera blocked the IgE-binding much better then rPhl p 1. The ability for inhibition is with 89P5 and KLHP5 almost alike. Table 2 shows the inhibition (in %) of all 19 patients.







TABLE 2







% inhibition of 19 patients' IgE-binding to rPhl p 1


after incubation with rabbit anti-rPhl p 1, r89P5 and anti-KLHP5


antisera









% inhibition












patient
rPhl p 1
r89P5
KLHP5
















1
32.343
68.291
68.213



2
29.373
64.915
61.509



3
10.367
59.469
66.270



4
28.087
73.906
71.330



5
13.808
49.358
45.372



6
22.597
66.259
67.331



7
5.375
26.667
18.902



8
22.478
42.612
47.979



9
5.019
39.822
56.837



10
13.756
53.878
63.047



11
26.444
58.430
57.944



12
25.795
67.243
62.458



13
41.330
75.694
79.517



14
35.543
85.714
87.012



15
45.796
84.255
75.185



16
32.641
76.508
77.412



17
26.483
63.171
47.735



18
19.229
85.750
86.642



19
31.142
62.428
71.086










Example 9
T-Cell Proliferation of Mouse Spleen Cells after Antigen Stimulation
Groups of three mice were immunized with KLH, KLHP5 and KLHP5edc. Groups of 4 mice were immunized four times with 89VP1, r89P5 and 89P5edc, and 2 mice were immunized with peptide 5 only (5 μg each). Spleen cells were taken 10 days after the last immunization and single cell cultures were stimulated in triplicates in 96 well plates with peptide 5 (P5), 89VP1, KLH, Con A and a medium as a positive and negative control, respectively. After four days radioactive [3H]thymidine 0.5 μCi was added to each well. Cells were then harvested with a Packard Cell Harvester onto unifilter plates after 15 hours. Cell-associated radioactivity was measured with a beta-counter. Stimulation indices where calculated and are shown at the y-axis. The antigen which was used for stimulation is shown on the x-axis. Each box represents the data of the antigen which was used for immunization of the mice (FIG. 8). All values above two count as positive. The KLH and KLHP5 immunized mice are only positive when stimulated with KLH and the peptide 5 mice are completely negative. The KLHP5edc group is also negative which corresponds to the ELISA results. Cells from r89P5, 89P5edc and 89VP1 immunized mice proliferated only after stimulation with 89VP1. The naive control mouse shows no proliferation in all cases. These results show that T-cell epitopes are provided by the carrier 89VP1 and not by the peptide 5.
Example 10
Detection of 14VP1-, 89VP1- and rPhl p 1-Specific Antibodies in Human Sera Obtained in Autumn and Winter by ELISA
Five human sera of randomly chosen persons were taken in autumn and five in winter. The IgG1, IgG2, IgG4 and IgA antibody level against 14VP1, 89VP1 and rPhl p 1 was determined by ELISA as described in example 5. Human IgG1, IgG2, IgG4 and IgA were detected (BD Pharmingen) 1:1000 with sheep anti mouse IgG POX-coupled (Amersham Bioscience) 1:2000. A high anti-14VP1 and 89VP1 IgG1 titer of sera taken in autumn and winter could be detected (FIG. 9). The anti-rPhl p 1 IgG1 antibody titer was much lower. IgG2, IgG4 and IgA antibodies could be detected in all cases at a very low level. The VP1 proteins of the different HRV strains are closely related and cross-reactivity has been shown in other studies.
Example 11
Anti-89VP1 and Anti-rPhl p 1 Antibodies of Phl p 1 Allergic Patients
Sera of five Phl p 1 allergic patients were taken and an ELISA experiment was performed as described in example 5. ELISA plates were coated with rPhl p 1 and 89VP1 and the specific IgM, IgG1, IgG2, IgG3, IgG4, IgA and IgE antibody titer were determined (FIG. 10). More anti-89VP1 IgG1 antibodies than anti-rPhl p 1 IgG1 antibodies could be detected.
Example 12
Detection of Anti-14VP1 Antibody Binding to the Whole Virus by Western Blot Analysis
The IgG antibody binding of sera of the 14VP1 injected rabbit to the whole virus was confirmed by using the whole HRV14 virus (lane 2 and 5) and 5 μg purified 14VP1 (lane 3 and 6) as control. The virus extract was separated by 12% SDS-Page and blotted onto nitrocellulose membrane. Rabbit anti-14VP1 antiserum (lane 1-3) 1:500 and preimmune serum (lane 4-6) 1:500 were tested for binding to HRV14 and 14VP1. Bound IgG was detected with 125I-labelled donkey anti-rabbit antibody and visualized by autoradiography (FIG. 11).
The binding of 14VP1-antiserum could be detected at the same seize (33.6 kD) as 14VP1.
Example 13
Anti-14VP1 Antibodies Neutralization of Intact Human Rhinovirus 14
The tissue culture dosis 50 (TCD50) of HRV14 was determined. Therefore, a virus dilution from 1:102-1:108 in MEM-Eagle 1% FCS and 40 mM MgCl2 was performed and incubated in 24 well plates at 34° C. in a humidified 5% CO2 atmosphere together with HeLa Ohio cells for three days. A control without the virus was also spread out.
The cytotoxic effect of the virus was visualized with crystal violet after three days and the TCD50 (the dilution where 50% of the cells are dead) was calculated.
Serum dilutions and virus (100TCD50) in row A and B were incubated at 34° C. After 2 hours cells were spread out in all wells. D5 and D6 are serum controls. The experimental schema is shown in FIG. 12. The neutralization effect of the antibodies was detected after three days with crystal violet (FIG. 12).
Example 14
Characteristics of Phl p 5-Derived Synthetic Peptides
Peptides were synthesized using Fmoc-strategy with HBTU-activation (0.1 mmol small-scale cycles) on the Applied Biosystems peptide synthesizer Model 433A as described. (Focke et al. Faseb J (2001) 15:2042). After in-depth analysis of the Phl p 5 allergen six Phl p 5-derived peptides ranging from 31 (P1: 3026 Dalton) to 38 (P6: 3853 Dalton) amino acids in length which are rich in solvent-exposed amino acids were prepared (Table 3).
These peptides have isoelectric points between 4.32 and 8.98 and three of them (peptide 3, 4 and 6) may contain human T-cell epitopes.








TABLE 3







Characteristics of non-allergenic Phl p 5-derived



synthetic peptides. Position (in relation to the Phl p 5


molecule), sequence, length, molecular weight (MW),


isoelectric point (pI) and presence of T-cell epitopes


of the Phl p 5-derived peptides are displayed. The


cysteine residue added to facilitate the coupling


is marked in bold and underlined.















aa

aa


T-cell




position
Sequence
length
MW
pI
epitope


















Pept. 1
 98-128
CGAASNKAFAEGLSGEP-
32
3026
8.16
−



(SEQ ID

KGAAESSSKAALTSK


No. 9)


 


Pept. 2
26-58
ADLGYGPATPAAPAAGYT-
34
3068
4.37
−


(SEQ ID

PATPAAPAEAAPAGKC


No. 10)


 


Pept. 3
132-162
AYKLAYKTAEGATPEAKY-
32
3482
6.29
+


(SEQ ID

DAYVATLSEALRIC


No. 11)


 


Pept. 4
217-246
CEAAFNDAIKASTG-
31
3236
4.87
+


(SEQ ID

GAYESYKFIPALEAAVK


No. 12)


 


Pept. 5
252-283
TVATA-
33
3501
8.98
−


(SEQ ID

PEVKYTVFETALKKAITAM-


No. 13)

SEAQKAAKC


 


Pept. 6
176-212
CAEEVKVIPAGELQVIEK-
38
3853
4.66
+


(SEQ ID

VDAAFKVAATAANAAPANDK


No. 14)









Example 15
Phl p 5-Derived Peptides Lack IgE Reactivity and Allergenic Activity
15.1. Lack of IgE Reactivity
To analyze the IgE reactivity of the six Phl p 5-derived peptides, the isolated as well as KLH-coupled Phl p 5-derived peptides were compared with complete rPhl p 5 regarding IgE-binding capacity by ELISA using sera from 29 grass pollen allergic patients (Table 4).







TABLE 4







Serological characterization of 29 grass pollen allergic


patients and a non-allergenic control. Sex, age, total


serum IgE levels (kU/L), timothy extract-specific IgE (kUA/L),


IgE antibodies specific for rPhl p 5 and the 6 isolated (P1-P6) and


KLH-coupled (KLH-P1-KLH-P6) peptides were measured by ELISA


and ODs (optical densities) are shown. Dashes indicate the lack


of IgE reactivity to the isolated as well as to the KLH-coupled


peptides.











total
IgE
IgE (OD)





















IgE
timothy
rPhl








Patient
sex
age
(kU/L)
kUA/L)
p 5
P1
P2
P3
P4
P5
P6





















1
m
29
140.0
25.90
1.437
—
—
—
—
—
—


2
m
39
76.2
10.50
0.456
—
—
—
—
—
—


3
f
29
100.0
33.50
0.699
—
—
—
—
—
—


4
f
31
261.0
28.10
0.930
—
—
—
—
—
—


5
m
33
380.0
32.00
0.545
—
—
—
—
—
—


6
f
31
278.0
37.00
1.720
—
—
—
—
—
—


7
m
43
128.0
20.70
1.118
—
—
—
—
—
—


8
f
29
200.0
18.40
0.489
—
—
—
—
—
—


9
f
34
76.6
18.70
0.571
—
—
—
—
—
—


10
m
35
144.0
39.30
0.157
—
—
—
—
—
—


11
f
33
79.2
29.60
0.574
—
—
—
—
—
—


12
f
30
30.3
10.70
0.350
—
—
—
—
—
—


13
f
34
106.0
20.80
0.395
—
—
—
—
—
—


14
f
52
448.0
43.00
1.320
—
—
—
—
—
—


15
f
25
294.0
95.50
1.638
—
—
—
—
—
—


16
m
30
471.0
82.60
0.752
—
—
—
—
—
—


17
m
44
2000.0
100.00
2.500
—
—
—
—
—
—


18
f
30
168.0
66.60
0.806
—
—
—
—
—
—


19
m
42
512.0
50.30
1.175
—
—
—
—
—
—


20
f
28
253.0
54.00
1.954
—
—
—
—
—
—


21
m
30
315.0
100.00
1.054
—
—
—
—
—
—


22
f
42
401.0
89.50
2.297
—
—
—
—
—
—


23
f
28
100.0
82.10
1.802
—
—
—
—
—
—


24
m
42
52.5
3.52
0.885
—
—
—
—
—
—


25
m
34
136.0
6.11
2.036
—
—
—
—
—
—


26
m
30
31.2
9.17
1.909
—
—
—
—
—
—


27
m
36
24.9
4.34
0.233
—
—
—
—
—
—


28
f
41
41.5
2.19
0.281
—
—
—
—
—
—


29
f
51
370.0
90.10
1.296
—
—
—
—
—
—


NHS
m
39
0.0
0.00
0.065
—
—
—
—
—
—









ELISA plates (Nunc Maxisorp, Denmark) were coated with Phl p 5-derived peptides (5 μg/ml) or rPhl p 5 as control (5 μg/ml), washed and blocked. Subsequently, plates were incubated with 1:3 diluted sera from 29 grass pollen allergic patients and from a non-atopic individual overnight at 4° C. Grass pollen allergic patients suffering from allergic rhinoconjunctivitis and/or asthma were selected according to case history indicative for seasonal grass pollinosis and characterized by skin prick testing with timothy pollen extract and serological CAP-FEIA (Pharmacia Diagnostics, Uppsala, Sweden) testing. Total IgE levels in the sera were determined by CAP measurements (Pharmacia). IgE antibodies specific for rPhl p 5 were determined by ELISA. Sera from 29 grass pollen allergic patients and a non-atopic individual were used for IgE competition studies. The grass pollen allergic patients group consisted of 13 males and 16 females with a mean age of 35 years (ranging from 25-51 years) (Table 4).
Bound IgE antibodies were detected with a 1:1000 diluted alkaline-phosphatase-coupled mouse monoclonal anti-human IgE antibody (Pharmingen, Calif.).
Total IgE levels and grass pollen extract-specific IgE ranged from 24.9-2000 kU/L (mean: 262.7) and 2.2-100 kUA/L (mean: 41.5), respectively. All patients contained rPhl p 5-specific IgE antibodies ranging between 0.157-2.530 OD units (mean: 1.082 OD units), but none of the 29 patients showed IgE reactivity to any of the peptides (P1-P6) or to an equimolar mix of the peptides (OD≦0.08). This result demonstrates that no serum contained detectable IgE antibodies with specificity for any of the six Phl p 5 derived peptides.
15.2. Reduced Allergenic Activity of the Peptides as Detected by CD 203 C Expression on Basophils: Basophil Activation and Flow Cytometry:
The upregulation of CD 203 c has been described as a surrogate marker for allergen-induced basophil activation and degranulation (Hauswirth et al., J Allergy Clin Immunol 2002; 110:102). Therefore, the allergenic activity of complete rPhl p 5 allergen and an equimolar mix of peptides by measuring CD 203 c upregulation on basophils of grass pollen allergic patients was compared.
Peripheral blood cells were obtained from 3 allergic donors after informed consent had been given. Heparinized blood aliquots (100 μl) were incubated with serial dilutions of recombinant allergens (10-4 to 10 μg/ml), anti-IgE antibody (1 μg/ml) or control buffer (phosphate-buffered saline=PBS) for 15 minutes at 37° C. After incubation, cells were washed in PBS containing 20 mM EDTA. Cells were then incubated with 10 μl of PE-conjugated CD203c mAb 97A6 for 15 minutes at room temperature (RT). Thereafter, erythrocytes were lysed with 2 mL FACSTM Lysing Solution. Cells were then washed, resuspended in PBS, and analyzed by two-color flow cytometry on a FACScan (Becton Dickinson), using Paint-a-Gate Software. Allergen-induced upregulation of CD203c was calculated from mean fluorescence intensities (MFIs) obtained with stimulated (MFIstim) and unstimulated (MFIcontrol) cells, and expressed as stimulation index (MFIstim: MFIcontrol). An SI of ≧2.0 (≧2-fold upregulation) was considered indicative of a specific response.
As shown in FIG. 14 it was found that complete rPhl p 5 shows a dose-dependent (10-4 to 10 μg/mL) increase in expression of CD203c on peripheral blood basophils in a sensitized individual, whereas an equimolar mix of the peptides shows no effect.
Determination of CD 203c expression on basophils from grass-pollen allergic patients indicates that no allergenic activity can be observed with the Phl p 5 derived peptides.
Example 16
Immunization with Phl p 5 Derived Peptides Induces IgG Antibodies Reactive with rPhl p 5 and Natural Allergens from Different Grass Species
16.1. Recombinant Allergens and Allergen Extracts
Purified recombinant Phl p 5 were expressed in E. coli as described (Vrtala et al. J of Immunol (1993) 151:4773-4781).
Grass pollen from Phleum pratense, Lolium perenne, Poa pratensis, Dactylis glomerata, Secale cereale, Triticum aestivum, Avena sativa, Hordeum vulgare, Anthoxanthum odoratum were obtained from Allergon Pharmacia (Sweden), and an aqueous pollen extract was prepared as described (Vrtala et al., Int Arch Allergy Immunol (1993) 102:160-9.).
16.2. Immunization of Rabbits
HPLC-purified peptides were coupled to KLH (keyhole limpet hemocyanin, MW 4.5×103-1.3×107, Pierce, USA) according to the manufacturers advice and purified using a Conjugation Kit (Sigma, USA).
Rabbits were immunized with each of the KLH conjugated-peptides (200 μg/injection) and, for control purposes, with complete rPhl p 5 using Freunds complete and incomplete adjuvant (Charles River). Serum samples were obtained in four week intervals.
16.3. Reactivity of Rabbit Antibodies with Complete rPhl p 5 and Natural Allergens from Different Grass Species
In order to investigate whether antibodies induced after immunization with KLH-coupled peptides recognize the rPhl p 5, natural Phl p 5 and Phl p 5-related grass pollen allergens from other grass pollen species, ELISA experiments were performed.
For ELISA detection, plates (Nunc Maxisorp, Denmark) were coated with pollen allergen extracts (100 μg/ml: Phleum pratense, Lolium perenne, Poa pratensis Dactylis glomerata, Secale cereale, Triticum aestivum, Avena sativa, Hordeum vulgare, Anthoxanthum odoratum) or purified recombinant allergen (5 μg/ml: rPhl p 5). ELISA plates were washed and blocked and subsequently incubated with rabbit anti-peptide antisera and corresponding pre-immunsera diluted 1:2500. Bound rabbit IgG was detected with a HRP-coupled goat anti-rabbit Ig antiserum (Jackson Immunresearch, Pennsylvania). Results represent means of duplicate determination with an error of <5% (FIG. 13, Table 5).
Table 5: Crossreactivity of anti-Phl p 5 peptide antisera with rPhl p 5 and natural group 5 allergens from Phleum pratense, Lolium perenne, Poa pratensis, Dactylis glomerata, Secale cereale, Triticum aestivum, Avena sativa, Hordeum vulgare, Anthoxanthum odoratum. IgG reactivities (OD values) of peptide antisera (anti-P1 to anti-P6) to Phl p 5 and pollen extracts from grass pollen are displayed for 6 rabbits which were immunized with KLH-conjugated Phl p 5-derived peptides (P1-P6).










Crossreactivity of anti-peptide antisera with rPhl p 5


and grass pollen extracts














anti-




anti-



P1
anti-P2
anti-P3
anti-P4
anti-P5
P6

















rPhl p 5a
1.115
2.418
1.336
1.600
1.540
2.142


Phleum pratense
0.227
1.155
0.955
0.703
1.138
1.000


Lolium perenne
0.056
1.320
0.834
0.238
0.163
2.500


Poa pratensis
0.070
1.491
1.045
1.880
2.200
2.500


Dactylis glomerata
0.060
0.390
0.728
0.689
0.154
0.657


Secale cereale
0.090
0.292
0.777
0.676
0.162
0.843


Triticum aestivum
0.116
1.076
0.734
0.404
0.570
0.703


Avena sativa
0.150
0.790
1.029
0.551
0.224
1.494


Anthoxanthum
0.114
1.209
1.531
0.827
1.114
1.115


Hordeum vulgare
0.080
1.972
1.150
1.184
0.602
1.513









16.4. Immunization with Phl p 5-Derived Peptides Induces Cross-Reactive IgG Antibodies
Immunization with each of the peptides induced a robust Phl p 5-specific IgG response which became detectable four weeks after the first immunization and increased after the second immunization (FIG. 13). Immunization with peptide 2 induced the highest Phl p 5 specific IgG response followed by peptides 6, 4, 5 and 1 which induced the lowest response. With the exception of anti-peptide 1 antibodies which lacked reactivity with group 5 allergens in Lolium perenne, Poa pratensis, Dactylis glomerata, Secale cereale and Hordeum vulgare, the other peptide antisera cross-reacted with each of the grass pollen extracts tested (Table 5).
Example 17
Immunization with Phl p 5-Derived Peptides Induces IgG Antibodies which Inhibit the Binding of Crass Pollen Allergic Patients IgE to Phl p 5
17.1. Inhibition of Allergic Patients' IgE-Binding to rPhl p 5a by Peptide-Specific IgG
Information regarding the capacity of the peptides to induce blocking antibodies is important since blocking antibodies were shown to play a major role in immunotherapy.
In order to examine the ability of peptide-induced rabbit Ig to inhibit the binding of allergic patients IgE to complete rPhl p 5 ELISA, competition experiments were performed using sera from 29 grass allergic patients.
ELISA plates were coated with rPhl p 5 (1 μg/ml) and preincubated either with a 1:250 dilution of each of the anti-peptide antisera (anti-P1-anti-P6), a mixture of the anti-peptide antisera, an anti-rPhl p 5 antiserum or for control purposes, with the corresponding preimmune sera or a mixture of the preimmune sera. After washing, the plates were incubated with 1:3 diluted sera from 29 grass pollen allergic patients and bound IgE antibodies were detected with the alkaline phosphatase-coupled mouse monoclonal anti-human IgE antibody (Pharmingen). The percentage inhibition of IgE-binding achieved by preincubation with the anti-peptide antisera was calculated as follows: % inhibition of IgE-binding=100−ODI/ODP×100. ODI and ODP represent the extinctions after preincubation with the rabbit's immune and preimmune serum, respectively. Preincubation of Phl p 5 with peptide-induced rabbit IgG inhibited allergic patients IgE reactivity to a varying degree. The average degree of inhibition of IgE binding ranged from 19.3% for anti-peptide 6 IgG to 28.5% with anti-peptide 1 IgG. Rabbit antibodies raised agains complete Phl p 5 induced a mean inhibition of IgE binding of 43.6%.







TABLE 6







Rabbit anti-Phl p 5 peptide antisera inhibit serum


IgE-binding of timothy pollen allergic patients to Phl p 5. The


percentage inhibition of IgE-binding to Phl p 5 is displayed for


each patient after preincubation of Phl p 5 with anti-peptide


antisera (anti-P1-anti-P6), with a mix of the six anti-peptide


antisera (anti-P1-P6) or with an anti-rPhl p 5 antiserum. The


mean percentage inhibition is displayed in the bottom line.









% Inhibition of IgE-binding to Phl p 5 with antisera specific for















Patient
P1
P2
P3
P4
P5
P6
P1-P6
Phl p 5


















1
0
5
4
0
0
0
0
nd


2
1
10
4
0
0
0
0
nd


3
28
35
28
39
37
38
46
50


4
33
40
33
42
35
45
54
20


5
0
0
3
8
8
0
9
nd


6
46
34
39
47
47
34
21
56


7
41
48
46
49
50
45
49
60


8
41
8
34
18
39
0
0
 8


9
6
0
0
0
0
0
0
nd


10
34
46
30
35
39
42
48
38


11
28
0
32
0
28
9
0
nd


12
33
0
27
4
33
0
0
nd


13
30
31
29
0
29
11
15
 0


14
46
14
49
17
47
2
8
15


15
48
44
55
22
46
25
23
72


16
41
0
44
24
41
19
8
28


17
52
71
50
57
49
59
73
82


18
43
17
42
0
32
0
10
 0


19
5
17
19
16
7
0
4
nd


20
42
54
43
38
38
41
48
65


21
39
51
46
43
43
43
40
39


22
44
49
44
46
44
40
50
70


23
38
54
40
42
48
40
50
66


24
23
0
15
0
0
0
0
nd


25
0
35
0
8
4
28
43
nd


26
51
26
31
21
24
0
19
nd


27
14
15
3
0
9
11
25
nd


28
9
0
17
0
9
0
0
nd


29
10
44
11
31
21
28
24
73


mean
28.5
25.8
28.2
20.9
27.8
19.3
23.0
  43.6





nd: not done.






Example 18
Phl p 5-Derived Peptides Induce Low Specific Lymphoproliferative Responses
18.1. Lymphoproliferation Assays
In order to identify peptides with the lowest possible T-cell reactivity to minimize therapy-related side effects, the T-cell reactivity was examined by lymphoproliferation assays. Peripheral blood mononuclear cells (PBMC) were isolated from 2 grass pollen allergic patients by Ficoll (Amersham Pharmacia Biotech, UK) density gradient centrifugation. PBMC (2×105) were cultured in triplicates in 96-well plates (Nunclone; Nalge Nunc International, Denmark) in 200 μl serum-free Ultra Culture medium (BioWhittaker, Rockland, Me.) supplemented with 2 mM L-glutamine (SIGMA, USA), 50 μM beta-mercaptoethanol (SIGMA) and 0.1 mg gentamicin per ml (SIGMA) at 37° C. and 5% CO2 in a humidified atmosphere. Cells were stimulated with different concentrations of synthetic peptides (1.25, 0.6 and 0.3 μg per well) and, for comparison, with 4 U Interleukin-2 per well (Boehringer Mannheim, Germany) and with medium alone. After 6 d culture 0.5 μCi per well [3H]thymidine (Amersham Pharmacia Biotech) was added and 16 h thereafter incorporated radioactivity was measured by liquid scintillation counting using a microbeta scintillation counter (Wallac ADL, Germany). Mean cpm were calculated from the triplicates, and stimulation indices (SI) were calculated as the quotient of the cpm obtained by antigen or interleukin-2 stimulation and the unstimulated control.
PBMCs from timothy pollen allergic patients were stimulated with different concentrations of synthetic peptides. Stimulation indices with peptides were significantly lower than with IL2. Phl p 5-derived peptides induced low specific lymphoproliferative responses. The lowest response was seen with peptide 5 followed by peptide 4.
Example 19
Characteristics of Fel d 1-Derived Synthetic Peptides
In order to obtain peptides suitable for cat allergy vaccination, five peptides which are 30 to 36 amino acids long and cover the whole molecule were designed according to the known amino acid sequence of Fel d 1.
Peptides were synthesized using a Fmoc (9-fluorenylmethoxycarbonyl)-strategy with HBTU (2-(1H-benzotriazol-1-yl) 1,1,3,3 tetramethyluronium hexafluorophosphate)-activation (0.1 mmol small-scale cycles) on the Applied Biosystems peptide synthesizer Model 433A (USA). Preloaded PEG-PS (polyethylenglycol polystyrene) resins (0.15-0.2 mmol/g loading) (PerSeptive Biosystems, UK) were used as solid phase to build up the peptides. Chemicals were purchased from Applied Biosystems. Coupling of amino acids was confirmed by conductivity monitoring in a feedback control system. One cystein residue was added to peptides 1, 3, 4, and 5 to facilitate coupling to carriers (Table 7). Peptides were cleaved from the resins with a mixture of 250 μl distilled water, 250 μl triisopropylsilan (Fluka, Switzerland), 9.5 ml TFA for 2 h and precipitated in tert-butylmethylether (Fluka, Switzerland) (Focke 2001). The identity of the peptides was checked by mass-spectrometry and peptides were purified to >90% purity by preparative HPLC (PiChem, Austria).








TABLE 7







Molecular characteristics of Fel d 1-derived synthetic



peptides. Position within the native Fel d 1 molecule,


amino acid sequence, number of amino acids,


calculated molecular weight (MW) and theoretical


isoelectric point (pI) of the Fel d 1-derived synthetic


peptides are shown. All peptides are soluble in water.













Position
Amino acid sequence
aa length
MW
pI

















Pept. 1 SEQ
chain 1,
EICPAVKRDVDLFLTGTP-
35
3911
4.30



ID No. 15
aa 1-34
DEYVEQVAQYKALPVVC


 


Pept. 2 SEQ
chain 1,
LENARILKNCVDAKMTEEDKEN-
36
4083
4.72


ID No. 16
aa 35-70
ALSLLDKIYTSPLC


 


Pept. 3 SEQ
chain 2,
VKMAITCPIFYDVFFAVANG-
35
3835
4.56


ID No. 17
aa 1-34
NELLLDLSLTKVNAC


 


Pept. 4 SEQ
chain 2,
TEPERTAMKKIQDCYVENG-
30
3382
4.93


ID No. 18
aa 35-63
LISRVLDGLVC


 


Pept. 5 SEQ
chain 2,
CMTTISSSKD-
30
3246
4.78


ID No. 19
aa 64-92
CMGEAVQNTVEDLKLNTLGR









The five Fel d 1-derived synthetic peptides have molecular weights in the range of 3246 to 4083 Dalton and have calculated isoelectric points of from 4.30 to 4.93. All five peptides are watersoluble and Peptides 1, 2 and 3 may contain human T-cell epitopes (Table 7).







TABLE 8







Reduced IgE reactivity of Fel d 1-derived synthetic


peptides compared to rFel d 1





















cat












dander





total
specific





IgE
IgE
rFel d 1
Peptide 1
Peptide 2
Peptide 3
Peptide 4
Peptide 5


Patient
sex
age
(kU/l)
(kUA/l)
(O.D.)
(O.D.)
(O.D.)
(O.D.)
(O.D.)
(O.D.)




















1
f
36
>2000
48.2
2.314
0.112
—
—
—
0.056


2
m
27
798
62.2
2.255
0.069
0.123
—
—
0.140


3
m
33
153
9.48
1.394
—
—
—
—
—


4
m
25
122
13.2
1.194
1.998
0.113
0.186
—
0.073


5
f
42
267
42.1
1.793
0.074
—
—
—
0.677


6
f
35
494
37.0
2.007
—
—
—
—
0.204


7
m
27
129
31.3
2.259
—
—
—
—
0.149


8
m
36
1150
13.5
1.384
—
—
—
—
0.130


9
f
32
580
17.3
0.569
—
—
—
—
—


10
f
22
189
4.65
0.553
0.051
—
—
—
0.057


11
f
53
>2000
>100
2.838
0.504
—
—
—
0.644


12
f
75
4567
47.3
2.519
—
0.060
—
—
0.161


13
m
34
>2000
40.0
1.244
—
—
—
—
—


14
m
n.d.
n.d.
1.99
0.178
—
—
—
—
—


NHS
f
27
<2
<0.35
—
—
—
—
—
—









Example 20
Fel d 1-Derived Synthetic Peptides have Reduced IgE Reactivity Compared to rFel d 1 and Fel d 1-Derived Synthetic Peptides Lack Allergenic Activity
Serum IgE reactivity to the Fel d 1-derived synthetic peptides in order to identify hypoallergenic peptides suited for vaccination was investigated.
The diagnosis of IgE-mediated cat allergy was based on anamnesis, skin prick testing (Allergopharma, Reinbek, Germany) and measurement of total serum IgE as well as of cat dander-specific serum IgE (CAP-FEIA, Pharmacia Diagnostics, Sweden). Non-allergic persons were included for control purposes.
20.1. IgE-Binding Capacity Measured in ELISA Assays
The IgE-binding capacity of the five Fel d 1-derived synthetic peptides was compared with that of the complete rFel d 1 allergen using sera from 14 cat allergic patients. ELISA plates (Nunc Maxisorb, Denmark) were coated with Fel d 1-derived synthetic peptides or rFel d 1 as control (0.5 μg/well), washed and blocked. Plates were then incubated overnight at 4° C. with 1:5 diluted sera from cat allergic patients and from a non-atopic individual. Bound IgE antibodies were detected with a 1:2500 diluted horse-raddish-peroxidase labeled anti-human IgE antibody (KPL, USA).
Sera from 7 female and 7 male cat allergic patients at the age of 22 to 75 years were subjected to CAP-FEIA determinations. Measured total IgE levels ranged from 122 to >4000 kU/l and cat dander specific IgE levels from 1.99 to >100 kUA/l (Table 7). In ELISA assays the IgE reactivity of all 14 tested sera to the major cat allergen Fel d 1 was confirmed. Results were obtained as optical densities (OD) and ranged from 0.178 to 2.838 OD units. IgE reactivity of the 14 sera to Fel d 1-derived synthetic peptides was measured in the same ELISA assay. It was found that IgE-binding was retained for Peptides 1, 2, 3, and 5. IgE-binding was observed for 6/14 sera to Peptide 1, for 3/14 sera to Peptide 2, for 1/14 sera to Peptide 3 and for 10/14 sera to Peptide 5. Measured OD units were between 0.051 and 1.998 for Peptide 1, between 0.060 and 0.123 for Peptide 2, 0.186 for Peptide 3 and between 0.056 and 0.677 for Peptide 5. In summary, all measured OD units were considerably lower than the respective values measured for the whole Fel d 1 allergen.
This demonstrates that Fel d 1-derived synthetic peptides have a reduced IgE reactivity compared to the whole Fel d 1 allergen. Fel d 1-derived synthetic peptides can therefore be considered hypoallergenic, providing the advantage of reduced IgE-mediated side-effects, when used in SIT.
20.2. Specific Induction of Expression of Surface Markers CD203c and CD63 on Human Basophils (FIG. 17)
Since IgE-binding is a prerequisite but not ample for induction of type 1 allergic reactions that also require cross-link of effector cell bound specific IgE, the actual allergenic activity of Fel d 1-derived synthetic peptides was investigated in basophil activation assays. These assays detect an allergen-specific upregulation of surface markers CD203c and CD63, both recognized as markers for basopil activation (Hauswirth et al. J Allergy Clin Immunol. (2002) 110:102-109).
Heparinized blood samples were taken from 5 cat-allergic patients after informed consent had been given. Blood aliquots (100 μl) were incubated with serial dilutions of rFel d 1, Fel d 1-derived synthetic peptides as single components or as equimolar mixture, anti-IgE antibody or buffer (PBS) for 15 minutes at 37° C. After incubation, cells were washed in PBS containing 20 mM EDTA. Cells were then incubated with 10 μl of PE-conjugated CD203c mAb 97A6 and 20 μl of FITC-conjugated CD63 mAb CLB-gran12 for 15 minutes at room temperature. Thereafter, the samples were subjected to erythrocyte lysis with 2 ml FACSTM Lysing Solution. Cells were then washed, resuspended in PBS, and analyzed by two-color flow cytometry on a FACScan (Becton Dickinson, USA), using Paint-a-Gate Software. Allergen-induced upregulation of CD203c and CD63 was calculated from mean fluorescence intensities (MFIs) obtained with stimulated (MFIstim) and unstimulated (MFIcontrol) cells, and expressed as stimulation index (MFIstim:MFIcontrol). An SI of more than 2.0 (i.e. more than 2-fold upregulation) was considered indicative of a specific (diagnostic) response.
On basophils of all five studied cat-allergic patients (RR, EB, KC, MG and SM) stimulation with rFel d 1 induced an allergen-specific upregulation of surface markers CD203c and CD63. The upregulation of CD203c and CD63 was observed to be dose-dependent for 4/5 patients (RR, KC, MG and SM). For these patients CD203c stimulation indices ranged from 1.1 (SM) to 3.2 (RR) for the lowest tested concentration of 0.001 μg rFel d 1/ml and from 3.6 (KC) to 6.2 (RR) for the highest tested concentration of 10 μg rFel d 1/ml. CD63 stimulation indices determined in the same assay ranged from 1.1 (RR) to 2.0 (MG) for the lowest tested rFel d 1 concentration of 0.001 μg/ml and from 3.9 (RR) to 7.3 (MG) for the highest tested rFel d 1 concentration of 10 μg/ml. For Patient EB 0.001 μg/ml Fel d 1 were already sufficient to induce a high-level upregulation of surface markers CD203c and CD63 preventing an observation of dose-dependency of the surface marker upregulation.
Basophils from all five cat-allergic patients were probed with five increasing concentrations (0.005, 0.05, 0.5, 5 and 50 μg/ml) of an equimolar mix of the five Fel d 1-derived synthetic peptides. Basophils from patient RR were additionally probed with five increasing concentrations of the five single Fel d 1-derived synthetic peptides (0.001, 0.01, 0.1, 1 and 10 μg/ml). Peptides were found to be deficient in upregulating the basophil surface markers CD203c and CD63. Peptides were unable to induce any increased expression of CD203c and CD63 on cells of patient RR, KC and SM. A slight upregulation of CD203c (SI=2.3) and of CD63 (SI=2.5) could be detected for patient MG but only for the highest tested concentration of 50 μg peptide mixture/ml, while the lower concentrations applied had also no stimulating effect. A more pronounced upregulation of CD203c (SI=4.2) and CD63 (SI=4.3) was observed for patient EB but, again, only for the highest tested peptide mixture concentration. In both cases, patient MG and EB, the rate of upregulation after stimulation with peptides was considerably lower than the corresponding values for stimulation with the whole Fel d 1 allergen.
This demonstrates that Fel d 1-derived synthetic peptides provide the advantage of holding a lower allergenic activity than the whole Fel d 1 allergen. This is relevant for a decreased risk of IgE-mediated side-effects when Fel d 1-derived synthetic peptides are used in SIT.
Example 21
Immunization with Fel d 1-Derived Synthetic Peptides Induces IgG Antibodies Reactive with the Whole rFel d 1 Allergen
Fel d 1-derived synthetic peptides were shown to be deficient in IgE-binding. As candidate molecules for vaccination, which aims at the induction of allergen-specific IgG antibodies, peptides must retain specific allergen structures and must still be able to induce an IgG immune response specific for the whole allergen. In order to find out whether Fel d 1-derived synthetic peptides fulfill these requirements, immunization experiments in rabbits were performed.
Rabbits were immunized with uncoupled rFel d 1 and KLH-coupled Fel d 1-derived synthetic peptides. HPLC-purified peptides were coupled to KLH via their cysteine residues, using an Imject Maleimide Activated Immunogen Conjugation Kit (Pierce, USA).
Rabbits (New Zealand White rabbits) were immunized with the immunogens (200 μg/injection) using CFA (first immunization) and IFA (first booster injection after 4 weeks; a second booster injection with incomplete adjuvant was given after 7 weeks) (Charles River Breeding Laboratories, Germany). Rabbits were bled 8 weeks after the first immunization and in four-week intervals thereafter.
The induction of peptide- and rFel d 1-specific antibodies was monitored in ELISA assays. ELISA plates (Nunc Maxisorb, Denmark) were coated with rFel d 1 (0.5 μg/well), washed and blocked. Plate-bound rFel d 1 was then probed in duplicates with 1:1000 diluted rabbit antisera and the corresponding rabbit preimmune sera, and bound IgG was detected with an 1:2000 diluted hoseraddish-peroxidase labelled goat anti-rabbit antiserum (Jackson ImmunoResearch Inc., USA). Means of duplicates were calculated and showed errors of less than 5%.
Immunization with Fel d 1-derived synthetic peptides induces Fel d 1 reactive IgG antibodies. Eight weeks after the first immunization with each of the five Fel d 1-derived synthetic peptides, IgG antibodies reactive to the whole Fel d 1 allergen could be detected in each of the five rabbit antisera. IgG antibody levels remained at comparable levels in the subsequent bleedings (FIG. 16).
Anti-Peptide 1, anti-Peptide 2, anti-Peptide 4 and anti-Peptide 5 rabbit antisera showed IgG reactivities to Fel d 1 at about the same magnitude than the anti-Fel d 1 rabbit antiserum. Also the anti-Peptide 3 rabbit antiserum showed a distinct but a somewhat lower IgG reactivity to Fel d 1.
This indicates that all 5 Fel d 1-derived synthetic peptides are candidate molecules to induce an Fel d 1 specific IgG antibody response.
Example 22
Fel d 1-Derived Synthetic Peptides Induce Weaker Lymphoproliferative Responses than Fel d 1
Desired candidate molecules for improved SIT do not only offer the advantage of reduced IgE-mediated side effects but also of reduced T-cell mediated side effects. In order to investigate the T-cell activating properties of Fel d 1-derived synthetic peptides, lymphoproliferative assays were performed.
PBMCs were isolated from 7 cat-allergic patients by Ficoll (Amersham Pharmacia Biotech, UK) density gradient centrifugation. PBMC (2×105) were cultured in triplicates in 96-well plates (Nunclone, Nalgene Nunc International, Denmark) in 200 μl serum-free Ultra Culture medium (Cambrex, Belgium) supplemented with 2 mM L-glutamine (Sigma, USA), 50 μM β-mercaptoethanol (Sigma) and 0.1 mg gentamicin per ml (Sigma) at 37° C. using 5% CO2 in a humidified atmosphere. Cells were stimulated with different concentrations (5, 2.5, 1.25 and 0.6 μg/well) of rFel d 1 and Fe d 1-derived synthetic peptides as single components or as equimolar mixture and, for control purposes, with 4 U interleukin-2 or with medium alone. After 6 days of culture, 0.5 μCi per well 3H-thymidine (Amersham Pharmacia Biotech) was added and 16 h thereafter, incorporated radioactivity was measured by liquid scintillation counting using a microbeta scintillation counter (Wallac ADL, Germany), and mean cpm were calculated from the triplicates. The stimulation index (SI) was calculated as the quotient of the cpm obtained by antigen or interleukin-2 stimulation and the unstimulated medium control.
IL-2 stimulated proliferation of PBMC from all 7 tested cat-allergic patients, resulting in stimulation indices of 9.8 for RR, 5.2 for EB, 3.2 for KC, 6.7 for MG, 6.3 for SM, 15.7 for RA and of 13.9 for AR.
Fel d 1-derived synthetic peptides induced lower stimulation indices (Table 9).







TABLE 9







Fel d 1-derived synthetic peptides which on an


equimolar basis induce weaker lymphoproliferative responses than


Fel d 1 can be identified. PBMCs from 7 cat-allergic patients


were stimulated with serial dilutions of rFel d 1 or Fel d 1-derived


synthetic peptides as single components. Specific lymphoproliferative


responses are shown as stimulation indices.












5 μg/w
2.5 μg/w
1.25 μg/w
0.6 μg/w
















Patient RR
rFel d 1
2.6
1.8
1.5
1.9



Peptide 1
1.9
0.6
1.3
1.5



Peptide 2
2.1
1.3
2.0
1.6



Peptide 3
3.5
2.8
2.0
3.0



Peptide 4
2.5
2.4
1.5
0.8



Peptide 5
1.7
0.9
2.3
0.7


Patient EB
rFel d 1
8.2
2.9
1.6
1.5



Peptide 1
1.3
0.9
1.0
1.2



Peptide 2
2.4
1.7
1.8
1.6



Peptide 3
1.1
1.2
1.4
1.7



Peptide 4
3.6
3.6
3.2
2.3



Peptide 5
2.2
2.1
1.4
2.1


Patient KC
rFel d 1
0.8
1.2
1.3
5.2



Peptide 1
0.7
1.0
1.1
1.1



Peptide 2
1.2
1.5
1.0
1.1



Peptide 3
0.6
0.5
0.5
0.6



Peptide 4
1.6
1.4
1.3
1.1



Peptide 5
1.3
1.4
0.9
1.4


Patient MG
rFel d 1
2.9
2.3
2.3
2.2



Peptide 1
1.8
1.4
1.4
1.1



Peptide 2
1.2
1.3
1.4
0.9



Peptide 3
1.1
0.5
0.6
0.7



Peptide 4
1.1
1.5
1.8
1.0



Peptide 5
1.5
1.2
1.6
0.8


Patient SM
rFel d 1
2.3
1.6
1.8
1.1



Peptide 1
1.1
1.0
0.8
1.0



Peptide 2
1.8
1.1
1.3
1.2



Peptide 3
2.6
2.1
2.1
1.5



Peptide 4
1.9
1.6
1.7
1.1



Peptide 5
2.3
1.3
1.4
1.0


Patient RA
rFel d 1
3.2
1.2
2.4
1.2



Peptide 1
0.8
0.7
1.3
1.1



Peptide 2
1.2
0.5
1.7
1.6



Peptide 3
2.0
2.3
1.6
0.9



Peptide 4
3.0
1.3
1.1
0.6



Peptide 5
0.4
0.6
0.9
0.9


Patient AR
rFel d 1
1.4
0.6
0.9
1.0



Peptide 1
1.0
0.5
1.7
0.7



Peptide 2
0.7
0.6
0.9
0.6



Peptide 3
1.6
1.6
2.1
1.0



Peptide 4
1.0
0.7
0.7
0.6



Peptide 5
0.8
0.5
0.3
0.5









Example 23
IgG Antibodies Induced by Immunization with Fel d 1-Derived Synthetic Peptides Inhibit Binding of Cat-Allergic Patients IgE to the Whole Fel d 1 Allergen
The ability of peptide-induced rabbit Ig to inhibit the binding of allergic patients' IgE antibodies to complete rFel d 1 was examined in ELISA competition assays. ELISA plates (Nunc Maxisorb, Denmark) were coated with rFel d 1 (0.05 μg/well), washed and blocked. Plate-bound rFel d 1 was then preincubated with 1:100 diluted rabbit anti-peptide antisera (single anti-peptide antisera as well as a mixture of anti-peptide antisera were used), rabbit anti-rFel d 1 antiserum, and for control purposes also with the respective rabbit preimmune sera. After the plates had been washed, they were incubated with 1:5 diluted human sera from cat-allergic patients. Bound IgE antibodies were detected with a 1:2500 diluted horse-raddish-peroxidase labeled anti-human IgE antibody (KPL, USA). The percentage inhibition of IgE-binding achieved by preincubation with the anti-peptide antisera was calculated as follows: % inhibition of IgE-binding=100−O.D.I/O.D.P×100, with O.D.I being the measured optical density after preincubation with rabbit immune sera and O.D.P with rabbit preimmune sera.
Preincubation of ELISA-plate bound Fel d 1 with the 5 anti-peptide rabbit antisera resulted in inhibition patterns that varied between the 14 different tested sera from cat-allergic patients. Anti-Peptide 1 rabbit antiserum blocked patients' IgE-binding to Fel d 1 for 13/14 tested patients' sera, anti-Peptide 2 rabbit antiserum for 8/14, anti-Peptide 3 rabbit antiserum for 13/14, anti-Peptide 4 rabbit antiserum for 9/14 and anti-Peptide 5 rabbit antiserum for 5/14.
Also the range of inhibition showed variations between the different antisera. Among the single tested anti-peptide rabbit antisera, anti-Peptide 1 rabbit antiserum showed best inhibition rates with inhibitions from 0-55% (average 29%). With anti-Peptide 2 rabbit antiserum inhibition rates of 0-18% (average 5%) could be achieved, with anti-Peptide 3 rabbit antiserum of 0-29% (average 11%), with anti-Peptide 4 rabbit antiserum of 0-24% (average 8%) and with anti-Peptide 5 rabbit antiserum of 0-18% (average 4%).
A mix of all 5 anti-peptide rabbit antisera inhibited patients' IgE-binding to Fel d 1 most efficiently with inhibitions achieved for all patients' sera and inhibition rates of 25-84% (average 59%). These inhibitions were even more pronounced than that achieved by preincubation with the anti-Fel d 1 rabbit antiserum (Table 10).







TABLE 10







Rabbit antisera raised against Fel d 1-derived


synthetic peptides inhibit binding of human IgE to Fel d 1. The


percentage inhibition of IgE-binding to Fel d 1 achieved by pre-


incubation of Fel d 1 with rabbit antisera are shown for 14 patients


and as means. Preincubations were performed with 5 rabbit


antisera raised against the 5 Fel d 1-derived synthetic peptides


(anti-Peptide 1-5), a mix of the 5 anti-peptide antisera (Mix)


and an antiserum raised against Fel d 1 (anti-rFel d 1).




















Mix of









the 5








anti-



anti-
anti-
anti-
anti-
anti-
peptide
anti-


Patient
Peptide 1
Peptide 2
Peptide 3
Peptide 4
Peptide 5
antisera
rFel d 1

















1
48
18
29
20
18
78
64


2
24
0
8
0
0
67
43


3
55
11
5
17
8
84
74


4
38
7
11
24
8
66
49


5
10
5
5
12
0
54
48


6
33
0
12
5
0
68
46


7
6
1
10
5
0
58
45


8
44
3
17
10
0
60
53


9
26
17
12
15
16
53
43


10
0
0
10
0
0
31
26


11
38
0
0
0
0
52
56


12
47
0
22
0
7
75
51


13
27
2
8
0
0
56
41


14
16
0
6
5
0
25
25


mean
29
5
11
8
4
59
47









When the anti-Peptide 1 rabbit antiserum was combined with each of the other anti-peptide antisera, the inhibition of allergic patients IgE binding was substantially increased reaching almost values (e.g. anti-peptide 1+4:41%, anti-peptide 1+5:42%) obtained with anti-rFel d 1 antibodies (67%) (Table 11).












TABLE 11






anti-Peptide
anti-Peptide
anti-Peptide
anti-Peptide




1 +
1 +
1 +
1 +
anti-



anti
anti
anti
anti
rFel


Patient
Peptide 2
Peptide 3
Peptide 4
Peptide 5
d 1




















1
61
49
61
63
75


2
24
17
28
28
74


3
60
52
62
57
86


4
43
33
43
40
68


5
17
9
27
30
67


6
37
24
42
46
73


7
26
21
36
34
74


8
51
46
53
55
72


9
40
28
46
43
61


10
16
11
30
34
40


11
45
35
47
45
78


12
52
40
56
59
76


13
29
17
29
32
62


14
7
10
14
16
28


mean
36
28
41
42
67









Example 24
Bet v 1-Derived Peptides Induce Bet v 1-Specific IgG Responses Receiving T-Cell Help from Carrier-Derived T-Cell Epitopes and Reduce Bet v 1-Specific T-Cell Proliferation
Bet v 1-derived, surface-exposed B cell peptides have already been shown to induce a protective Bet v 1-specific IgG response in a mouse model of therapeutic and prophylactic vaccination (Focke M et al. Clin Exp Allergy (2004) 34:1525-1533). In Focke M et al. (2004), 6 Bet v 1-derived peptides were coupled to the carrier molecule KLH before immunization of mice. In the present example it is shown that the Bet v 1-specific IgG response induced with these peptides (Table 1) is driven by the help of T-cell epitopes derived from the carrier but not from the Bet v 1 allergen. It was surprisingly found that none of the Bet v 1-derived peptides having the sequences LFPKVAPQAISSVENIEGNGGPGTIKKISF (SEQ ID No. 20), GPGTIKKISFPEGFPFKYVKDRVDEVDHTN (SEQ ID No. 21) and VDHTNFKYNYSVIEGGPIGDTLEKISNEIK (SEQ ID No. 22) induced relevant T-cell responses, and it could be demonstrated that the majority of T-cell responses was directed against the carrier molecule, KLH (FIGS. 18 and 19). This finding is of great importance for the reduction of side effects during therapeutic vaccination and for reducing the risk of a potential sensitization during prophylactic vaccination. It has been demonstrated in the past that allergen-derived peptides lacking any IgE reactivity but containing allergen-derived T-cell epitopes induced side effects due to T-cell activation. Allergen-derived peptides lacking IgE and T-cell reactivity as exemplified for the Bet v 1 peptides, induce neither IgE nor T-cell mediated side-effects during therapeutic vaccination. When used for prophylactic vaccination, the peptides will induce a Bet v 1-specific protective IgG response without priming of Bet v 1-specific T-cells. This should minimize the risk of pre-priming an allergic immune response through the vaccine which might pave the road for a subsequent allergic sensitization.
In this example the allergen- and carrier-specific T-cell responses in a mouse model of therapeutic and prophylactic allergen vaccination were dissected. Bet v 1-derived peptides 2, 3, and 6 (Focke M et al. (2004)) were chosen and tested on whether they contain any of the known Bet v 1-specific T-cell epitopes in BALB/c mice. The mice were immunized as follows (Table 10 shows the sensitization and treatment protocol): Groups of BALB/c mice (n=5) were immunized with 10 μg recombinant Bet v 1 (Biomay, Austria) and/or a mixture of the synthetic Bet v 1-derived peptides 2, 3, and 6 (10 μg of each). Peptides were coupled to KLH as previously described (Focke M et al. (2004)). For immunization, Bet v 1 and the peptide mix were adsorbed to aluminium hydroxide (Alu-Gel-S, Serva, Germany) in a total volume of 150 μl/mouse.







TABLE 12





Sensitization and treatment protocol.


















Sensitization
Therapy


groups (n = 5)
(rBet v 1)
(peptides KLH)





no Sensitization/no Therapy (S−/T−)
—
—


Sensitization/no Therapy (S+/T−)
day 0, 20, 40
—


no Sensitization/Therapy (S−/T+)
—
day 60, 80, 100


Sensitization/Therapy (S+/T+)
day 0, 20, 40
day 60, 80, 100






Prophylaxis
Sensitization



(peptides KLH)
(rBet v 1)





no Prophylaxis/Sensitization (P−/S+)
—
day 60, 80, 100


Prophylaxis/no Sensitization (P+/S−)
day 0, 20, 40
—


Prophylaxis/Sensitization (P+/S+)
day 0, 20, 40
day 60, 80, 100









Allergen-, peptide-, and carrier-specific lymphoproliferation was analyzed in a T-cell proliferation assay. Spleens were removed under sterile conditions and homogenized. After lysis of erythrocytes, cells were washed and resuspended in complete medium (RPMI, 10% fetal calf serum, 0.1 mg/ml gentamycin, 2 mM glutamine). Single cell suspensions were plated into 96-well round-bottom plates at a concentration of 2×105 cells/well and stimulated with concavalin A (0.5 μg/well) as a positive control, rBet v 1 (2 μg/well), KLH (2 μg/well), the peptide mix (0.34 μg of each peptide/well) or the medium alone for 4 days. The cultures were pulsed with 0.5 μCi/well tritiated thymidine for 16 h and harvested. The proliferation responses were measured by scintillation counting. The ratio of the mean proliferation after antigen stimulation and medium control values, i.e. the stimulation index (SI), was calculated.
Interestingly, it could be shown that therapeutic vaccination with Bet v 1-derived peptides could reduce Bet v 1-specific proliferation in Bet v 1 sensitized mice (group S+/T+) compared to the sensitized but untreated group S+/T−. In the sensitized and treated group no peptide-specific proliferation could be measured, but according to the carrier effect, a KLH-specific proliferation was observed. The peptide vaccine alone (group S−/T+) induced mainly KLH-specific T-cells, but almost no Bet v 1-specific T-cell response (FIG. 18).
Prophylactic vaccination with the peptides induced almost no Bet v 1-specific proliferation (group P+/S−) compared to the Bet v 1-sensitized group P−/S+ but KLH-specific proliferation. In prophylactically vaccinated and subsequently sensitized mice (group P+/S+) Bet v 1 specific proliferation was remarkably reduced, furthermore, no peptide-specific response could be observed in any mouse group (FIG. 19).
Thus, it could be shown in an allergy mouse model that prophylactic vaccination with carrier-bound allergen-derived B cell peptides did not prime peptide-specific T-cells, almost no allergen-specific but carrier-specific T-cells. Prophylactic vaccination preceding allergic sensitization but also therapeutic vaccination of sensitized mice reduces allergen-specific T-cell proliferation.
Prophylactic treatment with Bet v 1-derived peptides induced Bet v 1-specific IgG responses without help by Bet v 1-specific T-cells. Furthermore, prophylactic treatment increased Bet v 1-specific IgG responses induced by the Bet v 1 allergen already 20 and 40 days after first sensitization (FIG. 20).
These results demonstrate that the peptide vaccine induces a Bet v 1-specific IgG response which can be boosted by allergen exposure.
Example 25
Der p 2-Derived Peptides Showing Reduced IgE Binding Capacity
The IgE binding capacity of Der p 2-derived peptides was determined as described in examples 15.1 and 20.1 employing the peptides according to table 13 and using sera of individuals suffering from house dust mite allergy.







TABLE 13







Der p 2-derived peptides













SEQ


Peptide
Position
Sequence
ID No.













Der p 2 Pep 1
1-33
DQVDVKDCANHEIKKVLVPG
96




CHGSEPCIIHRGK


 


Der p 2 Pep 2
21-51 
CHGSEPCIIHRGKPFQLEAV
97




FEANQNSKTAK


 


Der p 2 Pep 3
42-73 
EANQNSKTAKIEIKASIEGL
98




EVDVPGIDPNAC


 


Der p 2 Pep 4
62-103
EVDVPGIDPNACHYMKCPLV
99




KGQQYDIKYTWIVPKIAPKSEN


 


Der p 2 Pep 5
98-129
APKSENVVVTVKVMGDNGVL
100




ACAIATHAKIRD









The results clearly show that the Der p 2 derived peptides of the present invention exhibit significantly reduced IgE binding capacity.







TABLE 14







Results














rDer




peptide



p 2
peptide 1
peptide 2
peptide 3
peptide 4
5

















means
1.080
0.010
0.015
0.004
0.031
0.006


(n = 50)









Example 26
Variations in the Length of Peptides have No Effect on Peptides' IgE-Binding Capacity, T-Cell Reactivity and Immunogenicity
26.1. Design of Peptides
To study the effect of variation in peptides length on IgE-binding capacity, T-cell reactivity and immunogenicity variants of Phl p 5 derived peptides were designed by increasing the length of peptide 1 (P1) and decreasing the length of peptide 2 (P2) by a few amino-acids (Table 15).
Table 15: Position, sequence, length in number of aminoacids and molecular weight of synthetic Phl p 5 derived peptides (1, 2) and variants thereof (1a, 2b)







TABLE 15







Variants of PHL p 5-derived synthetic peptides















Molecular



Position

Number
Weight



aa
Sequence
of aa
(MW)















Peptide 1
 98-128
CGAASNKAFAEGLSG
32
3026




EPKGAAESSSKAAL




TSK


 


1a
 93-128
CFVATFGAASNKAFAE
37
3592




GLSGEPKGAAESSSKA




ALTSK


 


Peptide 2
26-58
ADLGYGPATPAAPAAG
34
3068




YTPATPAAPAEAAPAG




KC


 


2b
26-53
ADLGYGPATPAAPAAG
29
2644




YTPATPAAPAEAC









26.2. Lack of IgE-Reactivity
To analyse the IgE-reactivity of Phl p 5 derived peptides 1, 2 and their variants 1a, 2b dot-blot assays were performed applying 0.2 μg peptide/dot and using sera from 7 grass-pollen allergic patients (p1-p7) and the serum from a non-atopic individual (NHS). Bound IgE was detected with 125 I-labelled anti-human IgE (Phadia, Uppsala, Sweden). rPhl p 5 was used as positive control and HSA as negative control. Patients react with rPhl p 5 but not with the peptides and peptide variants (FIG. 21).
26.3. Lymphoproliferative Responses
PBMCs from 2 grass pollen allergic patients were stimulated with different concentrations of Phl p 5 derived peptides 1, 2, their variants 1a, 2b and for control purposes with rPhl p 5. Stimulation indices obtained with the peptides were significantly lower than those obtained with rPhl p 5 (FIG. 22).
26.4. Immunogenicity of Peptide Variants
Rabbits were immunized with KLH-coupled Phl p 5 derived peptides and variants. ELISA experiments were used to measure IgG reactivity of the obtained rabbit antisera to peptides and their variants (Table 16). Immunization with peptides and their variants induced cross-reactive IgG antibodies recognizing the peptide and the corresponding variant.
Table 16: Cross-reactivity of anti-Phl p 5 peptide antisera raised in rabbits by immunization with KLH-conjugated peptides. IgG reactivities of peptide antisera to peptides (1, 2) and variants (1a, 2b) are displayed. No reactivity was observed with preimmune-sera (pre P1, pre P1a, pre P2, pre P2b)

    
    
        a. Anti peptide 1 antiserum (anti P1) cross-reacts with peptide 1 variant (1a) and anti peptide 1a antiserum (anti P1a) cross-reacts with peptide 1.
        b. Anti peptide 2 antiserum (anti P2) cross-reacts with peptide 2 variant (2b) and anti peptide 2b antiserum (anti P2b) cross-reacts with peptide 2.
    
    









TABLE 16





Cross-reactivity of rabbit-antisera







a)














pre P1
anti P1
pre P1a
anti P1a







P1
0.041
0.880
0.052
0.947



P1a
0.038
0.705
0.048
0.859











b)














pre P2
anti P2
pre P2b
anti P2b







P2
0.089
1.168
0.042
1.175



P2b
0.075
0.954
0.053
1.122










26.5. Peptide Induced Rabbit Antisera Inhibit Grass Pollen Allergic Patients' IgE Binding to rPhl p 5
The ability of rabbit anti-peptide 2 and 2b IgGs to inhibit human IgE binding to rPhl p 5 was studied in competition ELISAs. ELISA plate bound rPhl p 5 was preincubated with anti P2, anti P2b and, for control purposes, with anti Phl p 5 antisera. Plates were then exposed to sera from 12 grass pollen allergic patients. The percentage of inhibition of IgE-binding to rPhl p 5 is displayed in Table 17. Anti peptide 2 and anti peptide 2b antisera inhibit patients' IgE binding to rPhl p 5 to the same extent.
Competition ELISAs were also performed with rabbit anti peptide 1 and 1a antisera. In example 17 (Immunization with Phl p 5 derived peptides induces IgG antibodies which inhibit the binding of grass pollen allergic patients IgE to Phl p 5) the anti peptide 1 (P1) antibodies inhibited patients IgE binding to Phl p 5 with a mean inhibition rate of 28.5%. Similar results were obtained with anti peptide 1a antiserum which gave an inhibition rate of 23.7%.
Table 17: Inhibition of patients' IgE binding to rPhl p 5 by anti peptide antisera. Anti peptide 2 and anti peptide 2b antisera inhibit patients' IgE binding to rPhl p 5 to the same extent. ELISA plate bound rPhl p 5 was preincubated with anti P2, anti P2b and, for control purposes, with anti Phl p 5 antisera. Plates were then exposed to sera from 12 grass pollen allergic patients. The percentage of inhibition of IgE-binding to rPhl p 5 is displayed.







TABLE 17







% Inhibition of IgE-binding












patient
anti P2
anti P2b
anti Phl p 5
















1
33.38
24.40
84.77



2
52.20
57.40
87.00



3
52.70
54.85
90.81



4
51.44
59.76
78.26



5
43.19
49.15
77.93



6
47.04
52.02
83.68



7
62.67
58.00
76.62



8
52.36
50.27
74.44



9
57.63
50.91
88.13



10
35.10
37.99
75.03



11
44.44
41.24
68.39



12
47.56
45.41
77.34










Example 27
Cross-Protection of Anti-VP1 Antibodies
Human rhinoviruses comprise over hundred different strains. In this neutralization test it is shown that the rhinovirus infection of one strain can also be inhibited by VP1 specific antibodies of another strain. HeLa cells were seeded out in the wells at equal density. 100TCD50 HRV14 was preincubated with dilutions of anti-14VP1- and anti-89VP1 antibodies (undiluted; 1:2-1:32 wells 1-6) and added to the wells in lane A and D, respectively. In lanes B and C 100TCD50 HRV 89 preincubated with dilutions of anti-14VP1- and anti-89VP1 antibodies, respectively, were added to the cells. After 3 days live cells were stained violet. Anti-89VP1 antibodies and anti-14VP1 antibodies block the infection of HRV14 in a comparable manner. The antibodies raised against 14VP1 and 89VP1 also inhibit the infection of HRV89 up to the same concentration.

## Claims

1. A recombinant hypoallergenic protein comprising a peptide fused or conjugated to a viral protein or a viral protein fragment, wherein the peptide is selected from the group consisting of amino acids 1 to 33 of SEQ ID NO:10; amino acids 2 to 31 of SEQ ID NO:12, and amino acids 2 to 38 of SEQ ID NO:14.

2. The recombinant hypoallergenic protein according to claim 1, wherein the peptide is fused to the N-terminus, the C-terminus or both of the viral protein or the viral protein fragment.

3. The hypoallergenic protein according to claim 1, wherein the viral protein is a capsid protein.

4. The hypoallergenic protein according to claim 1, wherein the viral protein is a human pathogenic virus protein.

5. The hypoallergenic protein according to claim 4, wherein the human pathogenic virus is a human rhinovirus 89 or human rhinovirus 14.

6. A nucleic acid molecule encoding the recombinant hypoallergenic protein according to claim 1.

7. A vector comprising the nucleic acid molecule according to claim 6.

8. The vector according to claim 7, wherein said vector is an expression vector.

9. The vector according to claim 7, wherein said vector is a bacterial vector.

10. An isolated cell, comprising the nucleic acid molecule according to claim 7.

11. A composition, comprising
the recombinant hypoallergenic protein according to claim 1;
and an adjuvant, a pharmaceutically acceptable carrier, a preservative or a combination thereof.

12. The composition according to claim 11, which comprises 10 ng to 1 g of said recombinant hypoallergenic protein.

13. The hypoallergenic protein according to claim 4, wherein the human pathogenic virus is a picornavirus.

14. The composition according to claim 12, wherein the viral protein is a capsid protein.

15. The composition according to claim 12, wherein the virus protein is a human rhinovirus 89 protein or a human rhinovirus 14 protein.

16. The composition according to claim 11, wherein the virus protein is a human pathogenic virus protein.

17. The recombinant hypoallergenic protein according to claim 1, wherein the peptide is amino acids 1 to 33 of SEQ ID NO:10.

18. The recombinant hypoallergenic protein according to claim 1, wherein the peptide is amino acids 2 to 31 of SEQ ID NO:12.

19. The recombinant hypoallergenic protein according to claim 1, wherein the peptide is amino acids 2 to 38 of SEQ ID NO:14.

20. A method for treating a grass pollen allergy in a subject in in need thereof, the method comprising administering the recombinant hypoallergenic protein according to claim 1.

21. A method for treating a grass pollen allergy in a subject in in need thereof, the method comprising administering the composition according to claim 11.