### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX- V9360) to Algae (Navicula pelliculosa)

PMRA Submission Number{

........ } EPA MRID Number43014201

Data Requirement:

Test material: DPX-V9360

Common name Nicosulfuron PMRA DATA CODE

EPA DP Barcode

OECD Data Point

EPA MRID

EPA Guideline {



### ............ }

386906 {



### ............ }

43014201

850.4500

Purity: 91.4%

Chemical name: IUPAC: 2-[[ 4,6-dimethoxypyrimidin-2-yl)-amino-carbonyl]aminosulfonyl]-N ,N-dimethyl-3- pyridinecarboxamide

CAS name: Not reported

CAS No.: 111991-09-04 Synonyms: Not reported

Primary Reviewer: Mia Howard

Staff Scientist, Cambridge Environmental, Inc.

Secondary Reviewer: Teri S. Myers

Environmental Scientist, CDM Smith

Primary Reviewer: Geoffrey Sinclair, Biologist

EPA/OPP/EFED/ERB 5

Secondary Reviewer(s): {

...................................... } {EPA/OECD/PMRA}

Reference/Submission No.: {.

.................... }

Company Code

Active Code

Use Site Category:

EPA PC Code 

### {

............ 

### }
{



### ............ } {



### ............ }

129008 [ForPMRA]
[For PMRA]
[For PMRA]

Date Evaluation Completed: {dd-mm-yyyy} 

### S . t

# ~.fir>~ tgna ure:
Date: 09/26/12

Signature:**~** , . · 

## 5

Date: 08/14/13**~**

Date: 8/26/13

Date: {

.. ;

......... }

CITATION: Hughes, J. 1992. DPX-V9360: Toxicity to Navicula pellicu/osa. An unpublished study performed by

Malcolm Pirnie, Inc., Tarrytown, NY. Laboratory project B382-153-6. Study sponsored by E.I. du Pont de

Nemours and Company, Wilmington, DE. Sponsor study number AMR 2320-92. Study completed 03 August

1992.

DISCLAIMER: This document provides guidance for EPA and PMRA reviewers on how to complete a data

evaluation record after reviewing a scientific study concerning the acute toxicity of a pesticide to aquatic

nonvascular plants. It is not intended to prescribe conditions to any external party for conducting this study nor to

establish absolute criteria regarding the assessment of whether the study is scientifically sound and whether the study satisfies any applicable data requirements. Reviewers are expected to review and to determine for each study,

on a case-by-case basis, whether it is scientifically sound and provides sufficient information to satisfy applicable

Page 1 of 15

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) to Algae ( Navicula pelliculosa )

PMRA Submission Number 

### {........} EPA MRID Number 43014201

Page 1 of 15 Data Requirement: PMRA DATA CODE {............}

EPA DP Barcode 386906

OECD Data Point {............}

EPA MRID 43014201

EPA Guideline 850.4500

Test material: DPX

- V9360 Purity: 91.4%

Common name Nicosulfuron

Chemical name: IUPAC : 2

- [[4,6

- dimethoxypyrimidin

- 2

- yl)

- amino

- carbonyl]aminosulfonyl]

- N,N

- dimethyl

- 3

- pyridinecarboxamide

CAS name : Not reported

CAS No. : 111991

- 09

- 04 Synonyms : Not reported

Primary Reviewer: Mia Howard Signature: Staff Scientist, Cambridge Environmental, Inc. Date: 09/26/ 12

Secondary Reviewer: Teri S. Myers Signature: Environmental Scientist, CDM Smith Date: 08/14 /13

Primary Reviewer: Geoffrey Sinclair, Biologist Date: 8/26/13

EPA/ OPP/EFED/ERB 5

Secondary Reviewer(s): {......................................} Date: {............} {EPA/OECD/PMRA}

Reference/Submission No.: {.....................}

Company Code {............} [For PMRA]

Active Code {............} [For PMRA] Use Site Category: {............} [For PMRA]

EPA PC Code 129008

Date Evaluation Completed: {dd

- mm

- yyyy}

CITATION : Hughes, J. 1992. DPX

- V9360: Toxicity to Navicula pelliculosa . An unpublished study performed by Malcolm Pirnie, Inc., Tarrytown, NY. Laboratory project B382

- 153

- 6. Study sponsored by E.I. du Pont de Nemours and Company, Wilmington, DE. Sponsor study number AMR 2320

- 92. Study completed 03 August

1992.

DISCLAIMER : This document provides guidance for EPA and PMRA reviewers on how to complete a data evaluation record after reviewing a scientific study concerning the acute toxicity of a pesticide to aquatic nonvascular plants. It is not intended to prescribe condition s to any external party for conducting this study nor to establish absolute criteria regarding the assessment of whether the study is scientifically sound and whether the study satisfies any applicable data requirements. Reviewers are expected to review a nd to determine for each study,

on a case

- by

- case basis, whether it is scientifically sound and provides sufficient information to satisfy applicable

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) to Algae ( Navicula pelliculosa )

PMRA Submission Number 

### {........} EPA MRID Number 43014201

Page 2 of 15 data requirements. Studies that fail to meet any of the conditions may be accepted, if appropriate; simil arly, studies that meet all of the conditions may be rejected, if appropriate. In sum, the reviewer is to take into account the totality of factors related to the test methodology and results in determining the acceptability of the study.

EXECUTIVE SUMMA RY :

In a 120

- hour acute toxicity study, cultures of freshwater diatom, Navicula pelliculosa

, were exposed to nicosulfuron (DPX

- V9360) at nominal concentrations of 0 (negative and solvent controls) and 30 μg/L under static

conditions. Measured concentratio ns were <5 (<LOD, negative and solvent controls) and 36 μg/L. The NOAEC and

EC 50 /IC 50 values based on cell density were <36 and >36 μg/L , respectively. The NOAEC and EC 50 /IC 50 values based on growth rate were <36 and > 36 μg/L, respectively. The NOAEC and EC 50 /IC 50 values based on biomass

were 36 and >36 μg/L respectively. The % growth inhibition of cell density in the treated algal culture as compared

to the negative control was 19%.

There were no compound related phytotoxic effects.

This toxicity study is classified as scientifically sound. It does not satisfy the guideline requirement for an acute algal ( Navicula pelliculosa ) toxicity study because it was designed under a prior registration with a lower applica tion rate. The application rate under the current registration request is double what was used for this study.

T his study does however provide valuable information and is classified as acceptable.

Results Synopsis

Test Organism: Freshwater diatom, Navicula pelliculosa Test Type (Flow

- through, Static, Static Renewal): Static

Cell density

EC 05 : Not calculable 95% C.I.: N/A

EC 50 : > 36 μg/L 95% C.I.: Not calculable

NOAEC: <36 μg/L Probit Slope: N/A

Growth rate

EC 05 : Not calculable 95% C.I.: N/A

EC 50 : > 36 μg/L 95% C. I.: Not calculable

NOAEC: <36 μg/L Probit Slope: N/A

Biomass

EC 05 : Not calculable 95% C.I.: N/A

EC 50 : > 36 μg/L 95% C.I.: Not calculable

NOAEC: 36 μg/L Probit Slope: N/A Endpoint(s) Effected: Cell density, growth rate

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) to Algae ( Navicula pelliculosa )

PMRA Submission Number 

### {........} EPA MRID Number 43014201

Page 3 of 15 I. MATERIALS AND METHODS

GUIDELINE FOLLOWED: The study followed a protocol which was designed to comply with the US

EPA’s Pesticide Assessment Guidelines and the Draft Guidelines for Non

Target Plant Testing for Registration of Pesticides in Canada. The following deviations from the US EPA OCSPP 850.4500 guideline (2012)

are noted:

1. Analytical verification was only performed at test initiation. It is recommended that the concentration

of test substance be verified at minimum at test initiation and termination.

2. The pH and total organic carbon, particulate matter, metal, pesticide, and chlorine contents of the dilution water were not reported.

3. This appears to be a limit test based on the estimated environmental concentration (EEC) that would result in an application r ate of 35 grams a.i. / hectare. The calculated EEC from the study is 24 ug/L. The tested concentration of 36 ug/L exceeds this. EPA regulations stipulates however that the limit test must be based on the highest application rate on the label rather than the consequent EEC. Additionally the application rate used to calculate this limit test is from 1992. Label changes as of 2011 suggest additional tests per EPA guidance are necessary with the higher application rate (calculated as 71 g / hectare

.

T hese deviations do not impact the validity of this study.

COMPLIANCE: Si gned and dated GLP, Quality Assurance and Data Confidential ity statements were provided. The study was performed in compliance w ith the FIFRA GLP Standards, 40 CFR Part 160.

A. MATERIALS:

1. Test material DPX

- V9360

Description: White solid

Lot No./Batch No. : Lot #31

Purity: 91.4%

Stability of compound

under test conditions: Reported as stable. Analytical verification was only performed at test

initiation. (OECD recommends water solubility, stability in water and light, pKa, Pow, and vapor pressure of test compound)

Storage conditions of

test chemicals: Room temperature

Physicochemic al properties of nicosulfuron

.

Parameter Values Comments

Water solubility at 20 Ε C 9000 mg/L Solubility value is at pH= 6.3; the temperature of this

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) to Algae ( Navicula pelliculosa )

PMRA Submission Number 

### {........} EPA MRID Number 43014201

Page 4 of 15 Parameter Values Comments

value was not specified

Vapor pressure Not reported

UV absorption Not reported

pKa Not reported

Kow Not reported

2. Test organism :

Name: Freshwater diatom, Navicula pelliculosa

EPA requires a nonvascular species: For tier I testing, only one species, S. capricornutum , to be tested; for tier II testing, S. costatum, A. flos

- aquae, S. capricorntum, and a freshwater diatom is

tested.

OECD suggests the following species are considered suitable: S. capricornutum, S. subspicatus, and C. vulgaris. If other species are use d, the strain should be reported

Strain: Not reported

Source: In

- house cultures originally obtained from the University of Texas Culture Collection, Austin, Texas Age of inoculum : (at test initiation)

Method of cultivation: Maintained in synthetic algal assay procedure nutrient medium with silicon (AAP/Si)

B. STUDY DESIGN:

1. Experimental Conditions

a. Range

- finding study

– N/A.

b. Definitive Study

Table 1: Experimental Parameters

Parameter Details Remarks

Criteria

Acclimation period: Continuously cultured in the laboratory

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) to Algae ( Navicula pelliculosa )

PMRA Submission Number 

### {........} EPA MRID Number 43014201

Page 5 of 15 Parameter Details Remarks

Criteria

Culturing media and conditions: (same as test or not)

Health: (any mortality observed) Same as test

None reported EPA recommends two week acclimation period.

OECD recommends an amount of algae suitable for the inoculation of test

cultures and incubated under the conditions of the test and used when still exponentially growing, normally after an incubation period of about 3 days. When the algal cultures contain deformed or abn ormal cells, they must

be discarded.

Test system

Static/static renewal

Renewal rate for static renewal Static

N/A EPA expects the test concentrations to be renewed every 3 to 4 days (one renewal for the 7 day test, 3

- 4 renewals for the 14 day test).

Incubation facility Psycrotherm Controlled

Environmental Incubator Shaker,

Model G

- 27

Duration of the test 120 hours

EPA requires: 96

- 120 hours

OECD: 72 hours

Test vessel

Material: (glass/stainless steel)

Size:

Fill volume: Glass

500 mL

100 mL Erlenmeyer flasks fitted with foam

stoppers

OECD recommends 250 ml conical flasks are suitable when the volume of

the test solution is 100 ml or use a culturing apparatus.

Details of AAP/Si medium

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) to Algae ( Navicula pelliculosa )

PMRA Submission Number 

### {........} EPA MRID Number 43014201

Page 6 of 15 Parameter Details Remarks

Criteria

pH at test initiation: pH at test termination:

Chelator used:

Carbon source: Salinity ( for marine algae ): 7.50

- 7.51

8.16

- 8.38

Na 2 EDTAx2H 2 O

NaHCO 3 N/A OECD recommends the medium pH after equilibration with air is ~8 with less than .001 mmol/l of chelator if used.

EPA recommends 20X

- AAP and

c helating agents (e.g. EDTA) in the nutrient medium for optimum cell growth. Lower concentrations of chelating agents (down to one

- third of the normal concentration recommended for AAP medium) may

be used in the nutrient medium used for test solution preparation if it is suspected that the chelator will

interact with the test material. ASTM reference, E1415

- 91and D

3978

- 80 (reapproved 1987).

If non

- standard nutrient medium was used, detailed composition provided (Yes/No) A standard medium was used and a detailed composition was also provided

Dilution water source/type: pH: salinity ( for marine algae ): water pretreatment (if any): Total Organic Carbon: particulate matter:

metals: pesticides:

chlorine: ASTM Type I reagent grade water Not reported

N/A None reported Not reported Not reported Not reported Not reported Not reported EPA pH: Skeletonema costatum = ~8.0 Others = ~7.5 from beginning to end of the test. EPA salinity: 30

- 35 ppt. EPA is against the use of dechlorinated water.

OECD: pH is measured at beginning of
the test and at 72 hours, it should not normally deviate by more than one unit during the test.

Indicate how the test material is added to the medium (added directly or used stock solution) Used a stock solution prepared in

DMF

Aeration or agitation Continuous shaking at 100 rpm

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) to Algae ( Navicula pelliculosa )

PMRA Submission Number 

### {........} EPA MRID Number 43014201

Page 7 of 15 Parameter Details Remarks

Criteria

Initial cells density 1x10 4 cells/mL

EPA requires an initial number of 3,000

- 10,000 cells/mL. For Anabaena flos

aquae, cell counts on day 2 are not required.

OECD recommends that the initial cell concentration be approximately 10,000 cells/ml for S

. capricornutum and S

. subspicatus . When other species are

used the biomass should be comparable.

Number of replicates

Control:

Solvent control:

Treatments: 4

4

4 Fifth control replicates were used solely for the measurement of pH at

72 hours

EPA requires a negative and/or solvent control with 3 or more replicates per

doses. Navicula sp.tests should be conducted with four replicate.

OECD preferably three replicates at each test concentration and ideally twice that number of controls. When a vehicle
is used to solubilize the test substance, additional controls containing the vehicle at the highest concentration used

in the test.

Test concentrations

Nominal: 0 (negative and solvent controls) and 30 μg/L Limit test based on maximum application rate in Canada of 35 grams / hectare and “worst case conditions of 24 ug/L)

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) to Algae ( Navicula pelliculosa )

PMRA Submission Number 

### {........} EPA MRID Number 43014201

Page 8 of 15 Parameter Details Remarks

Criteria

Measured (at initiation) : <5 (<LOD, negative and solvent controls) and 36 μg/L EPA requires at least 5 test concentrations, with each at least 60% of the next higher one.

OECD recommends at least five concentrations arranged in a geometric
series, with the lowest concentration tested should have no observed effect on the growth of the algae. The highest

concentration tested should inhibit growth by at least 50% relatively t o the control and, preferably, stop growth completely.

Solvent (type, percentage, if used) 0.01% N,N

- dimethylformamide (DMF)

Method and interval of analytical

verification The concentration of the test

substance in the test solution was

measured at 0 hours using high performance liquid chromatography It is recommended that the

concentration of test substance be

verified at minimum at test initiation

and termination.

Test conditions Temperature: Photoperiod: Light intensity and quality: 24 ± 2°C

Continuous

4306 ± 646 lux , cool

- white fluorescent lighting EPA temperature: Skeletonema : 20 Ε C,

Others: 24

- 25 Ε C; EPA photoperiod: S. costatum 14 hr light/ 10 hr dark, Others: Continuous; EPA light:

Anabaena: 2.0 Klux (±15%), Others: 4

- 5 Klux (±15%)

OECD recommended the temperature in the range of 21 to25 o C maintained at ±

2 o C and continuous uniform illumination provided at approximately 8000 Lux measured with a spherical collector.

Reference chemical (if used)

name: concentrations: N/A

Other parameters, if any N/A

2. Observations:

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) to Algae ( Navicula pelliculosa )

PMRA Submission Number 

### {........} EPA MRID Number 43014201

Page 9 of 15 Table 2: Observation parameters

Parameters Details Remarks

Criteria

Parameters measured including the growth inhibition/other toxicity

symptoms Cell density

Growth rate

Biomass EPA recommends the growth of the algae expressed as the cell count per mL, biomass per volume, or degree of growth as determined by spectrophotometric means. Measurement technique for cell density and other end points Cell density was measured using the Coulter Counter, Model ZM, an electronic particle counter. The growth rate was ca lculated

from cell counts using a logarithmic equation. The

biomass was determined as the area under the growth curve. EPA recommends the measurement technique of cell counts or chlorophyll a

OECD recommends the electronic particle counter, microscope with counting chamber, fluorimeter, spectrophotometer, and colorimeter. (note: in order to provide useful measurements at low cell concentrations when using a spectrophotometer, it may be necessar y to use cuvettes with a light path of at least 4 cm).

Observation intervals Every 24 hours
EPA and OECD: every 24 hours.

Other observations, if any Cells were microscopically

examined at test termination for morphological abnormalities

Indicate whether there was an exponential growth in the control Yes. After 96and 120 hours, the

mean cell densities of the negative control wee 181x10 4

cells/mL and 219x10 4 cells/mL, respectively. EPA requires control cell count at termination to be 2X initial count or by a factor of at least 16 during the test.

OECD: cell concentration in control cultures should have increased by a factor of at least 16 within three days.

Were raw data included? Yes

II. RESULTS and DISCUSSION:

A. INHIBITORY EFFECTS:

After 120 hours, the mean cell density of the negative control was 218.8x10 4 cells/mL, yielding inhibitions compared to the negative control of

- 23 and 19% for the solvent control and 36 μg/L exposure group, respectively. There was not a significant difference between the two control groups. The treatment group mean was signific antly different than that of the pooled control group.

After 120 hours, the mean growth rate of the negative control was 0.539 , yielding inhibitions compared to the

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) to Algae ( Navicula pelliculosa )

PMRA Submission Number 

### {........} EPA MRID Number 43014201

Page 10 of 15 negative control of

- 1 and 4 % for the solvent control and 36 μg/L exposure group, respect ively. There was not a significant difference between the two control groups. The treatment group mean was significantly different

than that of the pooled control group.

After 120 hours, the mean biomass in the negative control was 3,325,688, yielding inhibitions compared to the negative control of

- 28 and 14% for the solvent control and 36 μg/L exposure group, respectively. There was not a significant difference between the two control groups. The treatment group mean was n ot significantly

different than that of the pooled control group.

There was not a major change in pH during the study. There were no phytotoxic effects.

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) to Algae ( Navicula pelliculosa )

PMRA Submission Number 

### {........} EPA MRID Number 43014201

Page 11 of 15 Table 3: Effect of nicosulfuron on algal growth ( freshwater diatom, Navicula pelliculosa )

Treatment

Nominal (and measured)

concentrations

μg/L Initial cell density (x10 4

cells/mL) Cell density (x10 4 cells/mL) at

72 hours 96 hours 120 hours

cell count % inhibition *

Negative control 1 36.7 181.2 218.8 N/A

Solvent control 1 59.6 222.8 268.7

- 23

30 (36) 1 25.2 169.8 176.3 19

Reference chemical (if used) N/A

*Calculated by the reviewer relative to the negative control mean

Table 4: Effect of nicosulfuron on algal growth (freshwater diatom, Navicula pelliculosa )

Treatment

Nominal (and measured)

concentrations

μg/L Growth rate %

inhibition * Cumulative

biomass (area under the curve) %

inhibition *

Negative control 0.539 N/A 3325688 N/A

Solvent control 0.543

- 1 4240400

- 28

30 (36) 0.517 4 2856565 14
*Calculated by the reviewer relative to the negative control mean

Table 5 : Statistical endpoint values.* (calculated by the study author relative to a pooled control group)

Statistical Endpoint Cell density G rowth rate Biomass

NOAEC ( μg /L) > 36 > 36 36

EC 50 (μg/L) Not calculated

Reference chemical, if used

NOAEC

IC 50 /EC 50 N/A

* Do not use this table, if the study was deemed unacceptable.

B. REPORTED STATISTICS:

The study author statistically analyzed the endpoints for cell density, growth rate, and biomass. The mean

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) to Algae ( Navicula pelliculosa )

PMRA Submission Number 

### {........} EPA MRID Number 43014201

Page 12 of 15 values of the two control groups were compared using a two

- tailed t

- test. Because there were no statistically significant differences, the negative and solvent control groups were combined in a pooled control that was compared to the treatment group in all subsequent analyses. The treatment groups were compared to the pooled control using one

- tailed t

- tests. All analyses were performed using SAS So ftware (SAS Institute, Cary, North Carolina).

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) to Algae ( Navicula pelliculosa )

PMRA Submission Number 

### {........} EPA MRID Number 43014201

Page 13 of 15 C. VERIFICATION OF STATISTICAL RESULTS:

Statistical Method: The study author statistically analyzed the endpoints for cell density, growth rate, and

biomass using mean measured exposure concentrations . The means of the two control groups were compared using two

- tailed t

- test. There were no significant differences between the means of the negative and solvent control groups. All further calculations were performed relative to the negative control group only . The NOAEC values were calculated using by comparing the treatment group to the negative control group via one

tailed t

- tests. All statistical analyses were performed using Microsoft Excel. The EC 50 values were empirically

estimated.

Cell density

EC 05 : Not calculable 95% C.I.: N/A

EC 50 : > 36 μg/L 95% C.I.: Not calculable

NOAEC: 36 μg/L Probit Slope: N/A

Growth rate

EC 05 : Not calculable 95% C.I.: N/A

EC 50 : > 36 μg/L 95% C.I.: Not calculable

NOAEC: 36 μg/L Probit Slope: N/A

Biomass

EC 05 : Not calculable 95% C.I.: N/A

EC 50 : > 36 μg/L 95% C.I.: Not calculable

NOAEC: 36 μg/L Probit Slope: N/A

D. STUDY DEFICIENCIES:

This appears to be a limit test based on the estimated environmental concentration (EEC) that would result in an application rate of 35 grams a.i. / hectare. The calculated EEC from the study is 24 ug/L. The tested concentration of 36 ug/L exceeds this. EPA regulations stipulate however that the limit test must be based on the highest application rate on the label rather than the consequent EEC. Additionally the application rate used to calculate this limit test is from 1992. Label changes as of 2011 suggest additional tests per EPA guidance are necessary with the higher application rate (calculated as 71 g / hectare )

.

E. REVIEWER **=** S COMMENTS:

The results of the reviewer agreed with those of the study author, even though the study author performed

an alyses relative to a pooled control group and the reviewer performed analyses relative to the negative control only. The study author did not directly state NOAEC values, but the reviewer extracted them based on the reported results of the t

- tests.

The in

- life phase of the definitive test was conducted from 16

- 21 March 1992.

F. CONCLUSIONS:

This toxicity study is classified as scientifically sound. It does not satisfy the guideline requirement for an acute

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) to Algae ( Navicula pelliculosa )

PMRA Submission Number 

### {........} EPA MRID Number 43014201

Page 14 of 15 algal ( Navicula pelliculosa ) toxicity stu dy because it was designed under a prior registration with a lower application rate. The application rate under the current registration request is double what was used for this study. It is therefore classified as supplemental qualitative.

After 120 hours, the most sensitive endpoint was cell density, which was inhibited by 19% in the treatment relative to the negative control.

Cell density

EC 05 : Not calculable 95% C.I.: N/A

EC 50 : > 36 μg/L 95% C.I.: Not calculable

NOAEC: <36 μg/L

Probit Slope: N/A

Growth rate

EC 05 : Not calculable 95% C.I.: N/A

EC 50 : > 36 μg/L 95% C.I.: Not calculable

NOAEC: <36 μg/L Probit Slope: N/A

Biomass

EC 05 : Not calculable 95% C.I.: N/A

EC 50 : > 36 μg/L 95% C.I.: Not calculable

NOAEC: 36 μg/L Probit Slope: N/A Endpoint(s) Effected: Cell density, growth rate

III. REFERENCES :

American Society for Testing and Materials (ASTM), 1991, "Standard Specification for R eagent Water, D 1193

- 77", Annual Book of ASTM

Standards. Vol 11.01.

Environmental Protection Agency, National Eutrophication Research Program, 1971, Algal Assay Bottle Test

Environmental Protection Agency, PR Notice 86

- 5, Notice to Producers, Formulators, Distributors and Registrants, July 29, 1986.

Environmental Protection Agency, Federal Insecticide, Fungicide and Rodenticide Act (FIFRA); Good Laboratory Practice Standards; Final Rule, 40 CFR Part 160, Federal Register, Vol. 54, No. 158, pp. 34052

- 34074, August 17, 1989.

Hoist, R.W. and T.C Ellwanger, 1982, Pe sticide Assessment Guidelines, Subdivision J Hazard Evaluation: Nontarget Plants, EPA

- 540/9

- 82

- 020.

Keddy, CJ., C. Boutin, and K. Freemark, 1991, Non

- target plant testing and evaluation for chemical pesticides: Draft guidelines for registration of pesticides in Canada.

### Data Evaluation Report on the Acute Toxicity of Nicosulfuron (DPX

- V9360) to Algae ( Navicula pelliculosa )

PMRA Submission Number 

### {........} EPA MRID Number 43014201

Page 15 of 15 Miller, W.E., J.C Greene and T. Shiroyama, 1978, The Seknastmm capricornu tum Printz Algal

- Assay Bottle Test, EPA

- 600/9

- 78

- 018.

Payne, A.G. and R.H. Hali, 1979, "A method for measuring algal toxicity and application to the safety as sessment of new chemical, "Aquatic Toxicology. ASTM STP 667, L.L. Testing and Materials, pp. 171

- 180.

Urban, DJ. and NJ. Cook, 1986, Hazard Evaluation Standard Evaluation Procedure, Ecological Risk Assessment, EPA 540/9

- 86

- 167.

APPENDIX I. OUTPUT OF THE REVIEWER’S STATISTICAL VERIFICATION

T

- tests performed in Microsoft Excel:



### t

- tests
Endpoint Groups compared p

- value
cell density controls 0.182915
cell density treatment vs. neg control 0.030644
growth rate controls 0.781678
growth rate treatment vs. neg control 0.047208

biomass controls 0.09706

biomass treatment vs. neg control 0.186677