13339 Hagers Ferry Road

Huntersville, NC 28078-7929

McGuire Nuclear Complex - MG03A2

Phone: 980-875-5245 Fax: 980-875-4349

Order Summary Report

Analytical Laboratory

Order Number: J12070244

Project Name: ALLEN Somerville Waste Water

Lab Contact: Jason C Perkins

Date: 8/3/2012

Customer Address: 253 Plant Allen Road

Customer Name(s): Don Scruggs, Robbin Jolly, Ray Lidke, Bill Kennedy

Belmont, NC 28012

Phone: 980-875-5348

Report Authorized By:

(Signature)

Program Comments:

Please contact the Program Manager (Jason C Perkins) with any questions regarding this report.

Data Flags & Calculations:

Any analytical tests or individual analytes within a test flagged with a Qualifier indicate a deviation from the method quality 

system or quality control requirement. The qualifier description is found at the end of the Certificate of Analysis (sample results) 

under the qualifiers heading. All results are reported on a dry weight basis unless otherwise noted.

Data Package:

This data package includes analytical results that are applicable only to the samples described in this narrative. An estimation of 

the uncertainty of measurement for the results in the report is available upon request. This report shall not be reproduced, except 

in full, without the written consent of the Analytical Laboratory. Please contact the Analytical laboratory with any questions. The 

order of individual sections within this report is as follows:

Job Summary Report, Sample Identification, Technical Validation of Data Package, Analytical Laboratory Certificate of Analysis, 

Analytical Laboratory QC Reports, Sub-contracted Laboratory Results, Customer Specific Data Sheets, Reports & 

Documentation, Customer Database Entries, Test Case Narratives, Chain of Custody (COC)

Certification:

The Analytical Laboratory holds the following State Certifications : North Carolina (DENR) Certificate #248, South Carolina 

(DHEC) Laboratory ID # 99005. Contact the Analytical Laboratory for definitive information about the certification status of 

specific methods. 

Page 1 of 48
Sample ID's & Descriptions:

Sample ID Plant/Station

Collection 

Date and Time Collected By Sample Description

2012015396 ALLEN 16-Jul-12 5:40 AM Chris Williams FGD Purge Eff 

2012015397 ALLEN 16-Jul-12 5:46 AM Chris Williams EQ Tank Eff 

2012015398 ALLEN 16-Jul-12 6:12 AM Chris Williams BioReactor 1 Inf 

2012015399 ALLEN 16-Jul-12 6:12 AM Chris Williams BioReactor 1 Inf BLANK 

2012015400 ALLEN 16-Jul-12 6:03 AM Chris Williams BioReactor 2 Inf 

2012015401 ALLEN 16-Jul-12 6:03 AM Chris Williams BioReactor 2 Inf BLANK 

2012015402 ALLEN 16-Jul-12 6:17 AM Chris Williams BioReactor 2 Eff 

2012015403 ALLEN 16-Jul-12 6:17 AM Chris Greene BioReactor 2 Eff BLANK 

2012015404 ALLEN 16-Jul-12 4:30 AM Chris Williams Filter Blk 

2012015405 ALLEN 16-Jul-12 6:17 AM Chris Williams BioReactor 2 Eff BLANK DUP

10 Total Samples

Page 2 of 48
COC and .pdf report are in agreement with sample totals 

and analyses (compliance programs and procedures).

All Results are less than the laboratory reporting limits.

All laboratory QA/QC requirements are acceptable.

The Vendor Laboratories have been qualified by the 

Analytical Laboratory

Yes No

Technical Validation Review

Checklist:

Yes No

Yes No

Report Sections Included:

Job Summary Report Sub-contracted Laboratory Results

Sample Identification Customer Specific Data Sheets, Reports, & Documentation

Technical Validation of Data Package Customer Database Entries

Analytical Laboratory Certificate of Analysis

Analytical Laboratory QC Report

Chain of Custody

Reviewed By: DataBase Administrator Date: 8/3/2012

Yes

Electronic Data Deliverable (EDD) Sent Separately

Page 3 of 48
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12070244

2012015396

Collection Date: 16-Jul-12 5:40 AM

Site: FGD Purge Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY

Vendor Parameter Complete 1 V_PRISM

INORGANIC IONS BY IC

Bromide 17 mg/L 5 50 EPA 300.0 23-Jul-12 17:51 JAHERMA

Chloride 2300 mg/L 50 500 EPA 300.0 23-Jul-12 17:51 JAHERMA

Sulfate 1400 mg/L 50 500 EPA 300.0 23-Jul-12 17:51 JAHERMA

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) 18.1 ug/L 2.5 50 EPA 245.1 02-Aug-12 12:16 AGIBBS

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 106 mg/L 0.5 10 EPA 200.7 20-Jul-12 13:52 DJSULL1

Calcium (Ca) 2160 mg/L 0.1 10 EPA 200.7 20-Jul-12 13:52 DJSULL1

Iron (Fe) 77.2 mg/L 0.1 10 EPA 200.7 20-Jul-12 13:52 DJSULL1

Lithium (Li) 0.149 mg/L 0.05 10 EPA 200.7 20-Jul-12 13:52 DJSULL1

Magnesium (Mg) 447 mg/L 0.05 10 EPA 200.7 20-Jul-12 13:52 DJSULL1

Manganese (Mn) 2.81 mg/L 0.05 10 EPA 200.7 20-Jul-12 13:52 DJSULL1

Potassium (K) 26.6 mg/L 1 10 EPA 200.7 20-Jul-12 13:52 DJSULL1

Sodium (Na) 24.5 mg/L 0.5 10 EPA 200.7 20-Jul-12 13:52 DJSULL1

DISSOLVED METALS BY ICP-MS

Manganese (Mn) 161 ug/L 10 10 EPA 200.8 20-Jul-12 14:00 KRICHAR

Selenium (Se) 647 ug/L 10 10 EPA 200.8 20-Jul-12 14:00 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) 187 ug/L 10 10 EPA 200.8 19-Jul-12 12:11 KRICHAR

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 19-Jul-12 12:11 KRICHAR

Chromium (Cr) 132 ug/L 10 10 EPA 200.8 19-Jul-12 12:11 KRICHAR

Copper (Cu) 130 ug/L 10 10 EPA 200.8 19-Jul-12 12:11 KRICHAR

Nickel (Ni) 182 ug/L 10 10 EPA 200.8 19-Jul-12 12:11 KRICHAR

Selenium (Se) 923 ug/L 10 10 EPA 200.8 19-Jul-12 12:11 KRICHAR

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 19-Jul-12 12:11 KRICHAR

Zinc (Zn) 219 ug/L 10 10 EPA 200.8 19-Jul-12 12:11 KRICHAR

SELENIUM SPECIATION

Vendor Parameter Complete 1 V_AS&C

TOTAL DISSOLVED SOLIDS

Vendor Parameter complete 1 V_PACE

Page 4 of 48
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12070244

2012015396

Collection Date: 16-Jul-12 5:40 AM

Site: FGD Purge Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

TOTAL SUSPENDED SOLIDS

Vendor Parameter Complete 1 V_PACE

2012015397

Collection Date: 16-Jul-12 5:46 AM

Site: EQ Tank Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) 15.5 ug/L 2.5 50 EPA 245.1 02-Aug-12 12:18 AGIBBS

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 113 mg/L 0.5 10 EPA 200.7 20-Jul-12 13:56 DJSULL1

Calcium (Ca) 1790 mg/L 0.1 10 EPA 200.7 20-Jul-12 13:56 DJSULL1

Iron (Fe) 64.0 mg/L 0.1 10 EPA 200.7 20-Jul-12 13:56 DJSULL1

Lithium (Li) 0.141 mg/L 0.05 10 EPA 200.7 20-Jul-12 13:56 DJSULL1

Magnesium (Mg) 463 mg/L 0.05 10 EPA 200.7 20-Jul-12 13:56 DJSULL1

Manganese (Mn) 2.38 mg/L 0.05 10 EPA 200.7 20-Jul-12 13:56 DJSULL1

Potassium (K) 26.0 mg/L 1 10 EPA 200.7 20-Jul-12 13:56 DJSULL1

Sodium (Na) 25.9 mg/L 0.5 10 EPA 200.7 20-Jul-12 13:56 DJSULL1

DISSOLVED METALS BY ICP-MS

Manganese (Mn) < 10 ug/L 10 10 EPA 200.8 20-Jul-12 14:04 KRICHAR

Selenium (Se) 626 ug/L 10 10 EPA 200.8 20-Jul-12 14:04 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) 140 ug/L 10 10 EPA 200.8 19-Jul-12 12:14 KRICHAR

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 19-Jul-12 12:14 KRICHAR

Chromium (Cr) 107 ug/L 10 10 EPA 200.8 19-Jul-12 12:14 KRICHAR

Copper (Cu) 112 ug/L 10 10 EPA 200.8 19-Jul-12 12:14 KRICHAR

Nickel (Ni) 149 ug/L 10 10 EPA 200.8 19-Jul-12 12:14 KRICHAR

Selenium (Se) 873 ug/L 10 10 EPA 200.8 19-Jul-12 12:14 KRICHAR

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 19-Jul-12 12:14 KRICHAR

Zinc (Zn) 172 ug/L 10 10 EPA 200.8 19-Jul-12 12:14 KRICHAR

2012015398

Collection Date: 16-Jul-12 6:12 AM

Site: BioReactor 1 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY

Vendor Parameter Complete 1 V_PRISM

Page 5 of 48
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12070244

2012015398

Collection Date: 16-Jul-12 6:12 AM

Site: BioReactor 1 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631

Vendor Parameter Complete 1 V_BRAND

MERCURY 1631 - DISSOLVED

Vendor Parameter Complete 1 V_BRAND

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 111 mg/L 0.5 10 EPA 200.7 20-Jul-12 14:00 DJSULL1

Calcium (Ca) 1820 mg/L 0.1 10 EPA 200.7 20-Jul-12 14:00 DJSULL1

Iron (Fe) < 0.1 mg/L 0.1 10 EPA 200.7 20-Jul-12 14:00 DJSULL1

Lithium (Li) 0.080 mg/L 0.05 10 EPA 200.7 20-Jul-12 14:00 DJSULL1

Magnesium (Mg) 360 mg/L 0.05 10 EPA 200.7 20-Jul-12 14:00 DJSULL1

Manganese (Mn) < 0.05 mg/L 0.05 10 EPA 200.7 20-Jul-12 14:00 DJSULL1

Potassium (K) 15.8 mg/L 1 10 EPA 200.7 20-Jul-12 14:00 DJSULL1

Sodium (Na) 25.9 mg/L 0.5 10 EPA 200.7 20-Jul-12 14:00 DJSULL1

DISSOLVED METALS BY ICP-MS

Manganese (Mn) < 10 ug/L 10 10 EPA 200.8 20-Jul-12 14:08 KRICHAR

Selenium (Se) 498 ug/L 10 10 EPA 200.8 20-Jul-12 14:08 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 10 ug/L 10 10 EPA 200.8 19-Jul-12 12:17 KRICHAR

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 19-Jul-12 12:17 KRICHAR

Chromium (Cr) 11.4 ug/L 10 10 EPA 200.8 19-Jul-12 12:17 KRICHAR

Copper (Cu) < 10 ug/L 10 10 EPA 200.8 19-Jul-12 12:17 KRICHAR

Nickel (Ni) < 10 ug/L 10 10 EPA 200.8 19-Jul-12 12:17 KRICHAR

Selenium (Se) 547 ug/L 10 10 EPA 200.8 19-Jul-12 12:17 KRICHAR

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 19-Jul-12 12:17 KRICHAR

Zinc (Zn) < 10 ug/L 10 10 EPA 200.8 19-Jul-12 12:17 KRICHAR

SELENIUM SPECIATION

Vendor Parameter Complete 1 V_AS&C

2012015399

Collection Date: 16-Jul-12 6:12 AM

Site: BioReactor 1 Inf BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631

Vendor Parameter Complete 1 V_BRAND

MERCURY 1631 - DISSOLVED

Vendor Parameter Complete 1 V_BRAND

Page 6 of 48
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12070244

2012015400

Collection Date: 16-Jul-12 6:03 AM

Site: BioReactor 2 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY

Vendor Parameter Complete 1 V_PRISM

MERCURY 1631

Vendor Parameter Complete 1 V_BRAND

MERCURY 1631 - DISSOLVED

Vendor Parameter Complete 1 V_BRAND

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 113 mg/L 0.5 10 EPA 200.7 20-Jul-12 14:04 DJSULL1

Calcium (Ca) 1830 mg/L 0.1 10 EPA 200.7 20-Jul-12 14:04 DJSULL1

Iron (Fe) < 0.1 mg/L 0.1 10 EPA 200.7 20-Jul-12 14:04 DJSULL1

Lithium (Li) 0.083 mg/L 0.05 10 EPA 200.7 20-Jul-12 14:04 DJSULL1

Magnesium (Mg) 359 mg/L 0.05 10 EPA 200.7 20-Jul-12 14:04 DJSULL1

Manganese (Mn) 0.062 mg/L 0.05 10 EPA 200.7 20-Jul-12 14:04 DJSULL1

Potassium (K) 18.3 mg/L 1 10 EPA 200.7 20-Jul-12 14:04 DJSULL1

Sodium (Na) 26.4 mg/L 0.5 10 EPA 200.7 20-Jul-12 14:04 DJSULL1

DISSOLVED METALS BY ICP-MS

Manganese (Mn) 56.8 ug/L 10 10 EPA 200.8 20-Jul-12 14:11 KRICHAR

Selenium (Se) 212 ug/L 10 10 EPA 200.8 20-Jul-12 14:11 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 10 ug/L 10 10 EPA 200.8 19-Jul-12 12:21 KRICHAR

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 19-Jul-12 12:21 KRICHAR

Chromium (Cr) < 10 ug/L 10 10 EPA 200.8 19-Jul-12 12:21 KRICHAR

Copper (Cu) < 10 ug/L 10 10 EPA 200.8 19-Jul-12 12:21 KRICHAR

Nickel (Ni) < 10 ug/L 10 10 EPA 200.8 19-Jul-12 12:21 KRICHAR

Selenium (Se) 231 ug/L 10 10 EPA 200.8 19-Jul-12 12:21 KRICHAR

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 19-Jul-12 12:21 KRICHAR

Zinc (Zn) < 10 ug/L 10 10 EPA 200.8 19-Jul-12 12:21 KRICHAR

SELENIUM SPECIATION

Vendor Parameter Complete 1 V_AS&C

2012015401

Collection Date: 16-Jul-12 6:03 AM

Site: BioReactor 2 Inf BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631

Vendor Parameter Complete 1 V_BRAND

Page 7 of 48
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12070244

2012015401

Collection Date: 16-Jul-12 6:03 AM

Site: BioReactor 2 Inf BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631 - DISSOLVED

Vendor Parameter Complete 1 V_BRAND

2012015402

Collection Date: 16-Jul-12 6:17 AM

Site: BioReactor 2 Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY

Vendor Parameter Complete 1 V_PRISM

INORGANIC IONS BY IC

Bromide 36 mg/L 5 50 EPA 300.0 23-Jul-12 16:57 JAHERMA

Chloride 3400 mg/L 50 500 EPA 300.0 23-Jul-12 16:57 JAHERMA

Sulfate 1400 mg/L 50 500 EPA 300.0 23-Jul-12 16:57 JAHERMA

MERCURY 1631

Vendor Parameter Complete 1 V_BRAND

MERCURY 1631 - DISSOLVED

Vendor Parameter Complete 1 V_BRAND

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 121 mg/L 0.5 10 EPA 200.7 20-Jul-12 14:08 DJSULL1

Calcium (Ca) 2020 mg/L 0.1 10 EPA 200.7 20-Jul-12 14:08 DJSULL1

Iron (Fe) 0.228 mg/L 0.1 10 EPA 200.7 20-Jul-12 14:08 DJSULL1

Lithium (Li) 0.092 mg/L 0.05 10 EPA 200.7 20-Jul-12 14:08 DJSULL1

Magnesium (Mg) 387 mg/L 0.05 10 EPA 200.7 20-Jul-12 14:08 DJSULL1

Manganese (Mn) 0.071 mg/L 0.05 10 EPA 200.7 20-Jul-12 14:08 DJSULL1

Potassium (K) 26.4 mg/L 1 10 EPA 200.7 20-Jul-12 14:08 DJSULL1

Sodium (Na) 28.9 mg/L 0.5 10 EPA 200.7 20-Jul-12 14:08 DJSULL1

DISSOLVED METALS BY ICP-MS

Manganese (Mn) 64.0 ug/L 5 5 EPA 200.8 20-Jul-12 14:15 KRICHAR

Selenium (Se) 5.10 ug/L 5 5 EPA 200.8 20-Jul-12 14:15 KRICHAR

Page 8 of 48
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12070244

2012015402

Collection Date: 16-Jul-12 6:17 AM

Site: BioReactor 2 Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 5 ug/L 5 5 EPA 200.8 19-Jul-12 12:24 KRICHAR

Cadmium (Cd) < 5 ug/L 5 5 EPA 200.8 19-Jul-12 12:24 KRICHAR

Chromium (Cr) < 5 ug/L 5 5 EPA 200.8 19-Jul-12 12:24 KRICHAR

Copper (Cu) < 5 ug/L 5 5 EPA 200.8 19-Jul-12 12:24 KRICHAR

Nickel (Ni) < 5 ug/L 5 5 EPA 200.8 19-Jul-12 12:24 KRICHAR

Selenium (Se) 5.50 ug/L 5 5 EPA 200.8 19-Jul-12 12:24 KRICHAR

Silver (Ag) < 5 ug/L 5 5 EPA 200.8 19-Jul-12 12:24 KRICHAR

Zinc (Zn) < 5 ug/L 5 5 EPA 200.8 19-Jul-12 12:24 KRICHAR

SELENIUM SPECIATION

Vendor Parameter Complete 1 V_AS&C

2012015403

Collection Date: 16-Jul-12 6:17 AM

Site: BioReactor 2 Eff BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631

Vendor Parameter Complete 1 V_BRAND

MERCURY 1631 - DISSOLVED

Vendor Parameter Complete 1 V_BRAND

2012015404

Collection Date: 16-Jul-12 4:30 AM

Site: Filter Blk 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

DISSOLVED METALS BY ICP-MS

Manganese (Mn) < 1 ug/L 1 1 EPA 200.8 20-Jul-12 12:37 KRICHAR

Selenium (Se) 2.23 ug/L 1 1 EPA 200.8 20-Jul-12 12:37 KRICHAR

2012015405

Collection Date: 16-Jul-12 6:17 AM

Site: BioReactor 2 Eff BLANK DUP

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631

Vendor Parameter Complete 1 V_BRAND

Page 9 of 48
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J12070244

2012015405

Collection Date: 16-Jul-12 6:17 AM

Site: BioReactor 2 Eff BLANK DUP

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631 - DISSOLVED

Vendor Parameter Complete 1 V_BRAND

Page 10 of 48
July 24, 2012

LIMS USE: FR - PROGRAM MANAGER

LIMS OBJECT ID: 92124528

92124528

Project:

Pace Project No.:

RE:

Program Manager

Duke Energy

,

J12070244

Dear Program Manager:

Enclosed are the analytical results for sample(s) received by the laboratory on July 17, 2012. The

results relate only to the samples included in this report. Results reported herein conform to the

most current TNI standards and the laboratory's Quality Assurance Manual, where applicable, unless

otherwise noted in the body of the report.

Analyses were performed at the Pace Analytical Services location indicated on the sample analyte

page for analysis unless otherwise footnoted.

If you have any questions concerning this report, please feel free to contact me.

Sincerely,

Kevin Godwin for

Kevin Herring

kevin.herring@pacelabs.com

Project Manager

Enclosures

REPORT OF LABORATORY ANALYSIS

This report shall not be reproduced, except in full,

without the written consent of Pace Analytical Services, Inc..

Page 1 of 9

Pace Analytical Services, Inc.

9800 Kincey Ave. Suite 100

Huntersville, NC 28078

(704)875-9092

Pace Analytical Services, Inc.

2225 Riverside Dr.

Asheville, NC 28804

(828)254-7176

Pace Analytical Services, Inc.

205 East Meadow Road - Suite A

Eden, NC 27288

(336)623-8921

Page 11 of 48
CERTIFICATIONS

Pace Project No.:

Project:

92124528

J12070244

Asheville Certification IDs

2225 Riverside Dr., Asheville, NC 28804

Florida/NELAP Certification #: E87648

Massachusetts Certification #: M-NC030

North Carolina Drinking Water Certification #: 37712

North Carolina Wastewater Certification #: 40

South Carolina Certification #: 99030001

Virginia Certification #: 00072

West Virginia Certification #: 356

Virgina/VELAP Certification #: 460147

REPORT OF LABORATORY ANALYSIS

This report shall not be reproduced, except in full,

without the written consent of Pace Analytical Services, Inc..

Page 2 of 9

Pace Analytical Services, Inc.

9800 Kincey Ave. Suite 100

Huntersville, NC 28078

(704)875-9092

Pace Analytical Services, Inc.

2225 Riverside Dr.

Asheville, NC 28804

(828)254-7176

Pace Analytical Services, Inc.

205 East Meadow Road - Suite A

Eden, NC 27288

(336)623-8921

Page 12 of 48
SAMPLE SUMMARY

Pace Project No.:

Project:

92124528

J12070244

Lab ID Sample ID Matrix Date Collected Date Received

92124528001 2012015396 Water 07/16/12 05:40 07/17/12 17:52

REPORT OF LABORATORY ANALYSIS

This report shall not be reproduced, except in full,

without the written consent of Pace Analytical Services, Inc..

Page 3 of 9

Pace Analytical Services, Inc.

9800 Kincey Ave. Suite 100

Huntersville, NC 28078

(704)875-9092

Pace Analytical Services, Inc.

2225 Riverside Dr.

Asheville, NC 28804

(828)254-7176

Pace Analytical Services, Inc.

205 East Meadow Road - Suite A

Eden, NC 27288

(336)623-8921

Page 13 of 48
SAMPLE ANALYTE COUNT

Pace Project No.:

Project:

92124528

J12070244

Lab ID Sample ID Method

Analytes

Analysts Reported Laboratory

92124528001 2012015396 SM 2540C LMD 1 PASI-A

SM 2540D LMD 1 PASI-A

REPORT OF LABORATORY ANALYSIS

This report shall not be reproduced, except in full,

without the written consent of Pace Analytical Services, Inc..

Page 4 of 9

Pace Analytical Services, Inc.

9800 Kincey Ave. Suite 100

Huntersville, NC 28078

(704)875-9092

Pace Analytical Services, Inc.

2225 Riverside Dr.

Asheville, NC 28804

(828)254-7176

Pace Analytical Services, Inc.

205 East Meadow Road - Suite A

Eden, NC 27288

(336)623-8921

Page 14 of 48
ANALYTICAL RESULTS

Pace Project No.:

Project:

92124528

J12070244

Sample: 2012015396 Lab ID: 92124528001 Collected: 07/16/12 05:40 Received: 07/17/12 17:52 Matrix: Water

Parameters Results Units Limit MDL DF Prepared Analyzed CAS No. Qual

Report

2540C Total Dissolved Solids Analytical Method: SM 2540C

Total Dissolved Solids 8180 mg/L 500 500 1 07/18/12 22:49

2540D Total Suspended Solids Analytical Method: SM 2540D

Total Suspended Solids 3630 mg/L 250 250 1 07/19/12 01:43

REPORT OF LABORATORY ANALYSIS

This report shall not be reproduced, except in full,

without the written consent of Pace Analytical Services, Inc..

Date: 07/24/2012 09:25 AM Page 5 of 9

Pace Analytical Services, Inc.

9800 Kincey Ave. Suite 100

Huntersville, NC 28078

(704)875-9092

Pace Analytical Services, Inc.

2225 Riverside Dr.

Asheville, NC 28804

(828)254-7176

Pace Analytical Services, Inc.

205 East Meadow Road - Suite A

Eden, NC 27288

(336)623-8921

Page 15 of 48
QUALITY CONTROL DATA

Pace Project No.:

Project:

92124528

J12070244

QC Batch:

QC Batch Method:

Analysis Method:

Analysis Description:

WET/21674

SM 2540C

SM 2540C

2540C Total Dissolved Solids

Associated Lab Samples: 92124528001

Parameter Units

Blank

Result

Reporting

Limit Qualifiers

METHOD BLANK: 797493

Associated Lab Samples: 92124528001

Matrix: Water

Analyzed

Total Dissolved Solids mg/L ND 25.0 07/18/12 22:46

Parameter Units

LCS

Result

% Rec

Conc. % Rec Limits Qualifiers

LABORATORY CONTROL SAMPLE: 797494

Spike LCS

Total Dissolved Solids mg/L 250 262 105 80-120

Parameter Units

Dup

Result

Max

Result RPD RPD Qualifiers

92124722001

SAMPLE DUPLICATE: 797504

Total Dissolved Solids mg/L 17000 17100 1 20

REPORT OF LABORATORY ANALYSIS

This report shall not be reproduced, except in full,

without the written consent of Pace Analytical Services, Inc..

Date: 07/24/2012 09:25 AM Page 6 of 9

Pace Analytical Services, Inc.

9800 Kincey Ave. Suite 100

Huntersville, NC 28078

(704)875-9092

Pace Analytical Services, Inc.

2225 Riverside Dr.

Asheville, NC 28804

(828)254-7176

Pace Analytical Services, Inc.

205 East Meadow Road - Suite A

Eden, NC 27288

(336)623-8921

Page 16 of 48
QUALITY CONTROL DATA

Pace Project No.:

Project:

92124528

J12070244

QC Batch:

QC Batch Method:

Analysis Method:

Analysis Description:

WET/21677

SM 2540D

SM 2540D

2540D Total Suspended Solids

Associated Lab Samples: 92124528001

Parameter Units

Blank

Result

Reporting

Limit Qualifiers

METHOD BLANK: 797521

Associated Lab Samples: 92124528001

Matrix: Water

Analyzed

Total Suspended Solids mg/L ND 2.5 07/19/12 01:38

Parameter Units

LCS

Result

% Rec

Conc. % Rec Limits Qualifiers

LABORATORY CONTROL SAMPLE: 797522

Spike LCS

Total Suspended Solids mg/L 250 246 98 80-120

Parameter Units

Dup

Result

Max

Result RPD RPD Qualifiers

92124188002

SAMPLE DUPLICATE: 797523

Total Suspended Solids mg/L 25.4 23.0 10 20

Parameter Units

Dup

Result

Max

Result RPD RPD Qualifiers

92124513001

SAMPLE DUPLICATE: 797524

Total Suspended Solids mg/L ND ND 20

REPORT OF LABORATORY ANALYSIS

This report shall not be reproduced, except in full,

without the written consent of Pace Analytical Services, Inc..

Date: 07/24/2012 09:25 AM Page 7 of 9

Pace Analytical Services, Inc.

9800 Kincey Ave. Suite 100

Huntersville, NC 28078

(704)875-9092

Pace Analytical Services, Inc.

2225 Riverside Dr.

Asheville, NC 28804

(828)254-7176

Pace Analytical Services, Inc.

205 East Meadow Road - Suite A

Eden, NC 27288

(336)623-8921

Page 17 of 48
QUALIFIERS

Pace Project No.:

Project:

92124528

J12070244

DEFINITIONS

DF - Dilution Factor, if reported, represents the factor applied to the reported data due to changes in sample preparation, dilution of

the sample aliquot, or moisture content.

ND - Not Detected at or above adjusted reporting limit.

J - Estimated concentration above the adjusted method detection limit and below the adjusted reporting limit.

MDL - Adjusted Method Detection Limit.

PRL - Pace Reporting Limit.

RL - Reporting Limit.

S - Surrogate

1,2-Diphenylhydrazine (8270 listed analyte) decomposes to Azobenzene.

Consistent with EPA guidelines, unrounded data are displayed and have been used to calculate % recovery and RPD values.

LCS(D) - Laboratory Control Sample (Duplicate)

MS(D) - Matrix Spike (Duplicate)

DUP - Sample Duplicate

RPD - Relative Percent Difference

NC - Not Calculable.

SG - Silica Gel - Clean-Up

U - Indicates the compound was analyzed for, but not detected.

N-Nitrosodiphenylamine decomposes and cannot be separated from Diphenylamine using Method 8270. The result reported for

each analyte is a combined concentration.

Acid preservation may not be appropriate for 2-Chloroethylvinyl ether, Styrene, and Vinyl chloride.

Pace Analytical is TNI accredited. Contact your Pace PM for the current list of accredited analytes.

TNI - The NELAC Institute.

LABORATORIES

PASI-A Pace Analytical Services - Asheville

REPORT OF LABORATORY ANALYSIS

This report shall not be reproduced, except in full,

without the written consent of Pace Analytical Services, Inc..

Date: 07/24/2012 09:25 AM Page 8 of 9

Pace Analytical Services, Inc.

9800 Kincey Ave. Suite 100

Huntersville, NC 28078

(704)875-9092

Pace Analytical Services, Inc.

2225 Riverside Dr.

Asheville, NC 28804

(828)254-7176

Pace Analytical Services, Inc.

205 East Meadow Road - Suite A

Eden, NC 27288

(336)623-8921

Page 18 of 48
QUALITY CONTROL DATA CROSS REFERENCE TABLE

Pace Project No.:

Project:

92124528

J12070244

Lab ID Sample ID QC Batch Method QC Batch Analytical Method

Analytical

Batch

92124528001 2012015396 SM 2540C WET/21674

92124528001 2012015396 SM 2540D WET/21677

REPORT OF LABORATORY ANALYSIS

This report shall not be reproduced, except in full,

without the written consent of Pace Analytical Services, Inc..

Date: 07/24/2012 09:25 AM Page 9 of 9

Pace Analytical Services, Inc.

9800 Kincey Ave. Suite 100

Huntersville, NC 28078

(704)875-9092

Pace Analytical Services, Inc.

2225 Riverside Dr.

Asheville, NC 28804

(828)254-7176

Pace Analytical Services, Inc.

205 East Meadow Road - Suite A

Eden, NC 27288

(336)623-8921

Page 19 of 48
This data package contains the analytical results for the project identified above and includes a Case Narrative, Sample 

Results and Chain of Custody. Unless otherwise noted, all samples were received in acceptable condition and processed 

according to the referenced methods. 

Data qualifiers are flagged individually on each sample. A key reference for the data qualifiers appears at the end of this case 

narrative.

NC Certification No. 402

SC Certification No. 99012

NC Drinking Water Cert No. 37735

Case Narrative

07/24/2012

Duke Energy Corporation (04)

Jay Perkins

13339 Hagers Ferry Road

Huntersville, NC 28078

Project: Allen Shay/Miller Creek

Project No.: J12070244

Lab Submittal Date: 07/17/2012

Prism Work Order: 2070379

VA Certification No. 1287

Please call if you have any questions relating to this analytical report.

Respectfully,

PRISM LABORATORIES, INC.

VP Laboratory Services Reviewed By

Data Qualifiers Key Reference:

HT Sample received and analyzed outside of the hold time.

MDL Method Detection Limit

BRL Below Reporting Limit

RPD Relative Percent Difference

*

Results reported to the reporting limit. All other results are reported to the MDL with values between MDL and 

reporting limit indicated with a J.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 1 of 9

Page 20 of 48
Sample Receipt Summary

07/24/2012

Prism Work Order: 2070379

Client Sample ID Lab Sample ID Matrix Date Sampled Date Received

2012015396/FGD Purge Eff 2070379-01 Water 07/16/12 07/17/12

2012015398/BioReactor 1 Inf 2070379-02 Water 07/16/12 07/17/12

2012015400/BioReactor 2 Inf 2070379-03 Water 07/16/12 07/17/12

2012015402/BioReactor 2 Eff 2070379-04 Water 07/16/12 07/17/12

Samples received in good condition at 5.6 degrees C unless otherwise noted.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 2 of 9

Page 21 of 48
Laboratory Report

07/24/2012

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation (04) Project: Allen Shay/Miller Creek

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 2070379-01

Prism Work Order: 2070379

Time Collected: 07/16/12 05:40

Time Submitted: 07/17/12 17:30

2012015396/FGD Purge Eff

J12070244

General Chemistry Parameters

pH 6.9 HT pH Units 1 *SM4500-H B 7/18/12 16:00 JAB P2G0342

Total Alkalinity 38 mg/L 5.0 0.66 1 *SM2320 B 7/18/12 15:45 JAB P2G0343

Carbonate Alkalinity BRL mg/L 5.0 0.66 1 *SM2320 B 7/18/12 15:45 JAB P2G0344

Bicarbonate Alkalinity 38 mg/L 5.0 0.66 1 *SM2320 B 7/18/12 15:45 JAB P2G0345

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 3 of 9

Page 22 of 48
Laboratory Report

07/24/2012

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation (04) Project: Allen Shay/Miller Creek

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 2070379-02

Prism Work Order: 2070379

Time Collected: 07/16/12 06:12

Time Submitted: 07/17/12 17:30

2012015398/BioReactor 1 Inf

J12070244

General Chemistry Parameters

pH 7.2 HT pH Units 1 *SM4500-H B 7/18/12 16:00 JAB P2G0342

Total Alkalinity 46 mg/L 5.0 0.66 1 *SM2320 B 7/18/12 15:45 JAB P2G0343

Carbonate Alkalinity BRL mg/L 5.0 0.66 1 *SM2320 B 7/18/12 15:45 JAB P2G0344

Bicarbonate Alkalinity 46 mg/L 5.0 0.66 1 *SM2320 B 7/18/12 15:45 JAB P2G0345

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 4 of 9

Page 23 of 48
Laboratory Report

07/24/2012

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation (04) Project: Allen Shay/Miller Creek

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 2070379-03

Prism Work Order: 2070379

Time Collected: 07/16/12 06:03

Time Submitted: 07/17/12 17:30

2012015400/BioReactor 2 Inf

J12070244

General Chemistry Parameters

pH 7.5 HT pH Units 1 *SM4500-H B 7/18/12 16:00 JAB P2G0342

Total Alkalinity 160 mg/L 5.0 0.66 1 *SM2320 B 7/18/12 15:45 JAB P2G0343

Carbonate Alkalinity BRL mg/L 5.0 0.66 1 *SM2320 B 7/18/12 15:45 JAB P2G0344

Bicarbonate Alkalinity 160 mg/L 5.0 0.66 1 *SM2320 B 7/18/12 15:45 JAB P2G0345

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 5 of 9

Page 24 of 48
Laboratory Report

07/24/2012

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation (04) Project: Allen Shay/Miller Creek

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 2070379-04

Prism Work Order: 2070379

Time Collected: 07/16/12 06:17

Time Submitted: 07/17/12 17:30

2012015402/BioReactor 2 Eff

J12070244

General Chemistry Parameters

pH 7.2 HT pH Units 1 *SM4500-H B 7/18/12 16:00 JAB P2G0342

Total Alkalinity 180 mg/L 5.0 0.66 1 *SM2320 B 7/18/12 15:45 JAB P2G0343

Carbonate Alkalinity BRL mg/L 5.0 0.66 1 *SM2320 B 7/18/12 15:45 JAB P2G0344

Bicarbonate Alkalinity 180 mg/L 5.0 0.66 1 *SM2320 B 7/18/12 15:45 JAB P2G0345

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 6 of 9

Page 25 of 48
7/24/12

Level II QC Report

13339 Hagers Ferry Road Project No: J12070244

Duke Energy Corporation (04) Project: Allen Shay/Miller Creek

Huntersville, NC 28078

Attn: Jay Perkins

Prism Work Order: 2070379

Time Submitted: 7/17/2012 5:30:00PM

Result Limit

Reporting

Units Level

Spike

Result

Source

%REC

%REC

Limits RPD

RPD

 Analyte Limit Notes 

General Chemistry Parameters - Quality Control

Batch P2G0342 - NO PREP

LCS (P2G0342-BS1) Prepared & Analyzed: 07/18/12 

pH 6.91 pH Units 6.860 101 99-101

Batch P2G0343 - NO PREP

Blank (P2G0343-BLK1) Prepared & Analyzed: 07/18/12 

Total Alkalinity BRL 5.0 mg/L

LCS (P2G0343-BS1) Prepared & Analyzed: 07/18/12 

Total Alkalinity 245 5.0 mg/L 250.0 98 90-110

LCS Dup (P2G0343-BSD1) Prepared & Analyzed: 07/18/12 

Total Alkalinity 248 5.0 mg/L 250.0 99 90-110 2 200

Batch P2G0344 - NO PREP

Blank (P2G0344-BLK1) Prepared & Analyzed: 07/18/12 

Carbonate Alkalinity BRL 5.0 mg/L

LCS (P2G0344-BS1) Prepared & Analyzed: 07/18/12 

Carbonate Alkalinity 245 5.0 mg/L 90-110

LCS Dup (P2G0344-BSD1) Prepared & Analyzed: 07/18/12 

Carbonate Alkalinity 248 5.0 mg/L 90-110 2 200

Batch P2G0345 - NO PREP

Blank (P2G0345-BLK1) Prepared & Analyzed: 07/18/12 

Bicarbonate Alkalinity BRL 5.0 mg/L

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 7 of 9

Page 26 of 48
7/24/12

Level II QC Report

13339 Hagers Ferry Road Project No: J12070244

Duke Energy Corporation (04) Project: Allen Shay/Miller Creek

Huntersville, NC 28078

Attn: Jay Perkins

Prism Work Order: 2070379

Time Submitted: 7/17/2012 5:30:00PM

Result Limit

Reporting

Units Level

Spike

Result

Source

%REC

%REC

Limits RPD

RPD

 Analyte Limit Notes 

General Chemistry Parameters - Quality Control

Batch P2G0345 - NO PREP

LCS (P2G0345-BS1) Prepared & Analyzed: 07/18/12 

Bicarbonate Alkalinity 245 5.0 mg/L 250.0 98 90-110

LCS Dup (P2G0345-BSD1) Prepared & Analyzed: 07/18/12 

Bicarbonate Alkalinity 248 5.0 mg/L 250.0 99 90-110 2 200

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 8 of 9

Page 27 of 48
Page 9 of 9

Page 28 of 48
 

3958 6th Ave NW • Seattle, WA 98107 • T: 206-632-6206 • F: 206-632-6017 • www.brooksrand.com • brl@brooksrand.com 

August 2, 2012 

Duke Energy 

ATTN: Jay Perkins

Scientific Support-Laboratory

13339 Hagers Ferry Road 

Huntersville NC 28078 

jcperkins@duke-energy.com 

labcustomer@duke-energy.com 

RE: Project DUK-HV1201 Client Project: J12070244 

Dear Mr. Perkins, 

On July 18, 2012, Brooks Rand Labs (BRL) received three (3) wastewater samples and three 

(3) corresponding field blanks. An aliquot was removed from each sample bottle and filtered into 

a separate container designed for dissolved mercury (Hg) analysis. The sample volume from 

the original container was logged-in for total Hg analysis. All samples were received, prepared, 

analyzed, and stored according to BRL SOPs and EPA methodology. 

The results were blank-corrected as described in the calculations section of the applicable 

SOP(s) and may be evaluated using adjusted reporting limits to account for sample aliquot size. 

Please refer to the Sample Results page for sample-specific detection limits and other details. 

Data used for regulatory purposes has a 24 hour filtration holding time requirement. Nonregulatory purposed data has a 48 hour filtration holding time. The samples were received 

within the 48 hour filtration requirement. 

The total Hg analysis for BioReactor 2 Inf Hg Blk (1229025-07) produced a detectable 

concentration of 65.5 ng/L. The sample was re-analyzed and confirmed the reported result. 

While this result was less than the associated field sample, the field sample result (178 ng/L) 

was within 10x the concentration of the blank and contamination was considered significant. All 

data was reported without additional qualification, aside from concentration qualifiers, and all 

associated quality control sample results met the acceptance criteria. 

BRL, an accredited laboratory, certifies the reported results of all analyses for which BRL is 

NELAP accredited meet all NELAP requirements. For more details, see the Report Information

page of the report. Please feel free to contact me if you have any questions regarding this 

report. 

Sincerely, 

 

 

Tiffany Stilwater 

Project Manager 

tiffany@brooksrand.com 

Page 29 of 48
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Definition of Data Qualifiers

(Effective 9/23/09)

Laboratory Accreditation

BRL is accredited by the National Environmental Laboratory Accreditation Program (NELAP) through the State of Florida 

Department of Health, Bureau of Laboratories (E87982) and is certified to perform many environmental analyses. BRL is 

also certified by many other states to perform environmental analyses. For a current list of our 

accreditations/certifications, please visit our website at <http://www.brooksrand.com/default.asp?contentID=586>. Results 

reported relate only to the samples listed in the report.

Report Information

BLK

BRL

BS

CAL

CCV

CRM

D

DUP

ICV

MSD

ND

NR

PS

REC

RPD

RSD

SCV

SOP

method blank 

Brooks Rand Labs

laboratory fortified blank

calibration standard

continuing calibration verification

certified reference material

dissolved fraction

duplicate

initial calibration verification

matrix spike duplicate

non-detect

non-reportable

post preparation spike

percent recovery

relative percent difference

relative standard deviation

secondary calibration verification

standard operating procedure

MDL

MRL

MS

method detection limit

method reporting limit

matrix spike

SRM

T

COC

standard reference material

total recoverable fraction

chain of custody record 

Common Abbreviations

These qualifiers are based on those previously utilized by Brooks Rand Labs, those found in the EPA SOW ILM03.0, 

Exhibit B, Section III, pg. B-18, and the USEPA Contract Laboratory Program National Functional Guidelines for 

Inorganic Superfund Data Review; USEPA; January 2010. These supersede all previous qualifiers ever employed by 

BRL.

Detected by the instrument, the result is > the MDL but ≤ the MRL. Result is reported and considered an estimate.

B

E 

An estimated value due to the presence of interferences. A full explanation is presented in the narrative.

H 

Holding time and/or preservation requirements not met. Result is estimated.

J 

Estimated value. A full explanation is presented in the narrative.

J-M

Duplicate precision (RPD) for associated QC sample was not within acceptance criteria. Result is estimated.

J-N

Spike recovery for associated QC sample was not within acceptance criteria. Result is estimated.

M 

Duplicate precision (RPD) was not within acceptance criteria. Result is estimated.

N 

Spike recovery was not within acceptance criteria. Result is estimated.

R 

Rejected, unusable value. A full explanation is presented in the narrative.

U 

Result is ≤ the MDL or client requested reporting limit (CRRL). Result reported as the MDL or CRRL.

X 

Result is not BLK-corrected and is within 10x the absolute value of the highest detectable BLK in the batch. 

Result is estimated.

Field Quality Control Samples

Please be notified that certain EPA methods require the collection of field quality control samples of an appropriate type 

and frequency; failure to do so is considered a deviation from some methods and for compliance purposes should only be 

done with the approval of regulatory authorities. Please see the specific EPA methods for details regarding required field 

quality control samples.

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 30 of 48
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Information

Sample Lab ID Report Matrix Type Sampled Received

BioReactor 1 Inf 1229025-01 Influent Sample 07/16/2012 07/18/2012

BioReactor 1 Inf 1229025-02 Influent Sample 07/16/2012 07/18/2012

BioReactor 1 Inf Hg Blk 1229025-03 DIW Field Blank 07/16/2012 07/18/2012

BioReactor 1 Inf Hg Blk 1229025-04 DIW Field Blank 07/16/2012 07/18/2012

BioReactor 2 Inf 1229025-05 Influent Sample 07/16/2012 07/18/2012

BioReactor 2 Inf 1229025-06 Influent Sample 07/16/2012 07/18/2012

BioReactor 2 Inf Hg Blk 1229025-07 DIW Field Blank 07/16/2012 07/18/2012

BioReactor 2 Inf Hg Blk 1229025-08 DIW Field Blank 07/16/2012 07/18/2012

BioReactor 2 Eff 1229025-09 Effluent Sample 07/16/2012 07/18/2012

BioReactor 2 Eff 1229025-10 Effluent Sample 07/16/2012 07/18/2012

BioReactor 2 Eff Hg Blk 1229025-11 DIW Field Blank 07/16/2012 07/18/2012

BioReactor 2 Eff Hg Blk 1229025-12 DIW Field Blank 07/16/2012 07/18/2012

BioReactor 2 Eff DUP 1229025-13 Effluent Sample 07/16/2012 07/18/2012

BioReactor 2 Eff DUP 1229025-14 Effluent Sample 07/16/2012 07/18/2012

Batch Summary

Analyte Lab Matrix Method Prepared Analyzed Batch Sequence

Hg Water EPA 1631 07/20/2012 07/23/2012 B121255 1200555

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 31 of 48
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Results

Sample Analyte Report Matrix Basis Result Qualifier MDL MRL Unit Batch Sequence

BioReactor 1 Inf

1229025-01 Hg Influent T 1720 3.16 8.42 ng/L B121255 1200555

1229025-02 Hg Influent D 461 3.03 8.08 ng/L B121255 1200555

BioReactor 1 Inf Hg Blk

1229025-03 Hg DIW T 0.15 U 0.15 0.40 ng/L B121255 1200555

1229025-04 Hg DIW D 0.15 U 0.15 0.41 ng/L B121255 1200555

BioReactor 2 Eff

1229025-09 Hg Effluent T 37.1 0.74 1.99 ng/L B121255 1200555

1229025-10 Hg Effluent D 3.17 0.15 0.40 ng/L B121255 1200555

BioReactor 2 Eff DUP

1229025-13 Hg Effluent T 40.2 0.78 2.09 ng/L B121255 1200555

1229025-14 Hg Effluent D 4.15 0.15 0.41 ng/L B121255 1200555

BioReactor 2 Eff Hg Blk

1229025-11 Hg DIW T 0.15 U 0.15 0.40 ng/L B121255 1200555

1229025-12 Hg DIW D 0.15 U 0.15 0.40 ng/L B121255 1200555

BioReactor 2 Inf

1229025-05 Hg Influent T 178 0.79 2.09 ng/L B121255 1200555

1229025-06 Hg Influent D 7.24 0.15 0.40 ng/L B121255 1200555

BioReactor 2 Inf Hg Blk

1229025-07 Hg DIW T 65.5 0.15 0.41 ng/L B121255 1200555

1229025-08 Hg DIW D 0.15 U 0.15 0.40 ng/L B121255 1200555

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 32 of 48
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Accuracy & Precision Summary

Batch: B121255

Analyte Native Spike Result Units REC & Limits RPD & Limits

Method: EPA 1631

Lab Matrix: Water

Sample

B121255-SRM1 Certified Reference Material (1229081, NIST 1641d 1000x dilution)

Hg 15.68 15.66 ng/L 100% 85-115

B121255-MS1 Matrix Spike (1229033-03)

Hg 18.99 96.17 121.9 ng/L 107% 71-125

B121255-MSD1 Matrix Spike Duplicate (1229033-03)

Hg 18.99 97.88 129.7 ng/L 113% 71-125 246%

B121255-MS2 Matrix Spike (1229037-01)

Hg 1.26 6.538 7.49 ng/L 95% 71-125

B121255-MSD2 Matrix Spike Duplicate (1229037-01)

Hg 1.26 6.560 8.39 ng/L 109% 71-125 2411%

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 33 of 48
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Method Blanks & Reporting Limits

Batch: B121255

Method: EPA 1631

Matrix: Water

Analyte: Hg

Sample Result Units

B121255-BLK1 0.15 ng/L

B121255-BLK2 0.07 ng/L

B121255-BLK3 0.07 ng/L

B121255-BLK4 0.07 ng/L

Average: 0.09 Standard Deviation: 0.04 MDL: 0.15

Limit: 0.50 Limit: 0.10 MRL: 0.40

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 34 of 48
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Instrument Calibration

Sequence: 1200555

Instrument: HGAAS-2 Method: EPA 1631

Date: 07/23/2012

Total Mercury and Mercury Speciation by CVAFS

Analyte: Hg

Lab ID True Value Result Units REC & Limits

1200555-IBL1 5.74 pg of Hg

1200555-IBL2 6.69 pg of Hg

1200555-IBL3 8.85 pg of Hg

1200555-IBL4 9.94 pg of Hg

1200555-CAL1 25.00 23.82 pg of Hg 95%

1200555-CAL2 100.0 103.5 pg of Hg 104%

1200555-CAL3 500.0 487.7 pg of Hg 98%

1200555-CAL4 2500 2627 pg of Hg 105%

1200555-CAL5 10000 9926 pg of Hg 99%

1200555-ICV1 1568 1566 pg of Hg 100% 85-115

1200555-CCV1 500.0 505.2 pg of Hg 101% 77-123

1200555-CCB1 10.0 pg of Hg

1200555-CCV2 500.0 536.1 pg of Hg 107% 77-123

1200555-CCV3 500.0 509.0 pg of Hg 102% 77-123

1200555-CCB2 6.77 pg of Hg

1200555-CCV4 500.0 511.2 pg of Hg 102% 77-123

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 35 of 48
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Containers

Lab ID: 1229025-01 Report Matrix: Influent

Sample: BioReactor 1 Inf Sample Type: Sample Received: 07/18/2012

Collected: 07/16/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71628390

10

Bottle FLPE Hg-T 500 mL none n/a Cooler

Lab ID: 1229025-02 Report Matrix: Influent

Sample: BioReactor 1 Inf Sample Type: Sample Received: 07/18/2012

Collected: 07/16/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71659890

20

Bottle FLPE Hg-T 250 mL none n/a Cooler

Comments: Split from 500 mL

Lab ID: 1229025-03 Report Matrix: DIW

Sample: BioReactor 1 Inf Hg Blk Sample Type: Field Blank Received: 07/18/2012

Collected: 07/16/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71628390

10

Bottle FLPE Hg-T 500 mL none n/a Cooler

Lab ID: 1229025-04 Report Matrix: DIW

Sample: BioReactor 1 Inf Hg Blk Sample Type: Field Blank Received: 07/18/2012

Collected: 07/16/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71659890

20

Bottle FLPE Hg-T 250 mL none n/a Cooler

Comments: split from 500 mL

Lab ID: 1229025-05 Report Matrix: Influent

Sample: BioReactor 2 Inf Sample Type: Sample Received: 07/18/2012

Collected: 07/16/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71628390

10

Bottle FLPE Hg-T 500 mL none n/a Cooler

Lab ID: 1229025-06 Report Matrix: Influent

Sample: BioReactor 2 Inf Sample Type: Sample Received: 07/18/2012

Collected: 07/16/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71659890

20

Bottle FLPE Hg-T 250 mL none n/a Cooler

Comments: split from 500mL

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 36 of 48
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Containers

Lab ID: 1229025-07 Report Matrix: DIW

Sample: BioReactor 2 Inf Hg Blk Sample Type: Field Blank Received: 07/18/2012

Collected: 07/16/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71353280

10

Bottle FLPE Hg-T 500 mL none n/a Cooler

Lab ID: 1229025-08 Report Matrix: DIW

Sample: BioReactor 2 Inf Hg Blk Sample Type: Field Blank Received: 07/18/2012

Collected: 07/16/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71659890

20

Bottle FLPE Hg-T 250 mL none n/a Cooler

Comments: split from 500mL

Lab ID: 1229025-09 Report Matrix: Effluent

Sample: BioReactor 2 Eff Sample Type: Sample Received: 07/18/2012

Collected: 07/16/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71628390

10

Bottle FLPE Hg-T 500 mL none n/a Cooler

Lab ID: 1229025-10 Report Matrix: Effluent

Sample: BioReactor 2 Eff Sample Type: Sample Received: 07/18/2012

Collected: 07/16/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71659890

20

Bottle FLPE Hg-T 250 mL none n/a Cooler

Comments: split from 500mL

Lab ID: 1229025-11 Report Matrix: DIW

Sample: BioReactor 2 Eff Hg Blk Sample Type: Field Blank Received: 07/18/2012

Collected: 07/16/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71353280

10

Bottle FLPE Hg-T 500 mL none n/a Cooler

Lab ID: 1229025-12 Report Matrix: DIW

Sample: BioReactor 2 Eff Hg Blk Sample Type: Field Blank Received: 07/18/2012

Collected: 07/16/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71659890

20

Bottle FLPE Hg-T 250 mL none n/a Cooler

Comments: split from 500mL

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 37 of 48
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Containers

Lab ID: 1229025-13 Report Matrix: Effluent

Sample: BioReactor 2 Eff DUP Sample Type: Sample Received: 07/18/2012

Collected: 07/16/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71629390

10

Bottle FLPE Hg-T 500 mL none n/a Cooler

Lab ID: 1229025-14 Report Matrix: Effluent

Sample: BioReactor 2 Eff DUP Sample Type: Sample Received: 07/18/2012

Collected: 07/16/2012

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71659890

20

Bottle FLPE Hg-T 500 mL none n/a Cooler

Comments: split from 500mL

Shipping Containers

Cooler

Tracking No: 535305192208 via FedEx

Temperature: 1.4 °C

Coolant Type: Ice

Description: Cooler

Damaged in transit? No

Returned to client? No

Custody seals present? No

Custody seals intact? No

COC present? Yes

Received: July 18, 2012 8:45

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 38 of 48
Page 39 of 48
 

July 25, 2012 

Jay Perkins 

Duke Energy Analytical Laboratory 

Mail Code MGO3A2 (Building 7405) 

13339 Hagers Ferry Rd. 

Huntersville, NC 28078 

(704) 875-5245 

Project: Allen Shay/MillerCreek (LIMS#J12070244) 

Dear Mr. Perkins, 

Attached is the report associated with four (4) aqueous samples submitted for selenium 

speciation analysis on July 17, 2012. The samples were received in a sealed cooler at -0.5°C on 

July 18, 2012. Selenium speciation analysis was performed via ion chromatography inductively 

coupled plasma collision reaction cell mass spectrometry (IC-ICP-CRC-MS). Any issues 

associated with the analysis are addressed in the following report. 

If you have any questions, please feel free to contact me at your convenience. 

 Sincerely, 

 

 Russell Gerads 

 Vice President 

 Applied Speciation and Consulting, LLC 

 

Page 40 of 48
Applied Speciation and Consulting, LLC 

Report prepared for: 

Jay Perkins 

Duke Energy Analytical Laboratory 

Mail Code MGO3A2 (Building 7405) 

13339 Hagers Ferry Rd. 

Huntersville, NC 28078 

Project: Allen Shay/MillerCreek (LIMS#J12070244) 

July 25, 2012 

1. Sample Reception 

Four (4) aqueous samples in 125mL HDPE bottles (provided by Applied Speciation and 

Consulting) were submitted for selenium speciation analysis on July 17, 2012. The samples 

were received on July 18, 2012 in a sealed container at -0.5°C. 

The samples were received in a laminar flow clean hood, void of trace metals contamination 

and ultra-violet radiation, and was designated a discrete sample identifier. An aliquot of each 

sample was filtered (0.45μm) and each filtrate was stored in a secure, monitored cryofreezer 

(maintained at a temperature of -80oC) until selenium speciation analysis could be performed 

via ion chromatography inductively coupled plasma collision reaction cell mass spectrometry 

(IC-ICP-CRC-MS). 

2. Sample Preparation 

All sample preparation is performed in laminar flow clean hoods known to be free from trace 

metals contamination. All applied water for dilutions and sample preservatives are 

monitored for contamination to account for any biases associated with the sample results. 

Selenium Speciation Analysis by IC-ICP-CRC-MS Prior to analysis, an aliquot of each 

sample was filtered with a syringe filter (0.45μm) and injected directly into a sealed 

autosampler vial. No further sample preparation was performed as any chemical alteration of 

a sample may shift the equilibrium of the system, resulting in changes in speciation ratios. 

3. Sample Analysis 

All sample analysis is preceded by a minimum of a five-point calibration curve spanning the 

entire concentration range of interest. Calibration curves are performed at the beginning of 

each analytical day. All calibration curves, associated with each species of interest, are 

Page 41 of 48
standardized by linear regression resulting in a response factor. All sample results are 

instrument blank corrected to account for any operational biases associated with the 

analytical platform. 

Prior to sample analysis, all calibration curves are verified using second source standards 

which are identified as initial calibration verification standards (ICV). 

Ongoing instrument performance is identified by the analysis of continuing calibration 

verification standards (CCV) and continuing calibration blanks (CCB) at a minimal interval 

of every ten analytical runs. 

Selenium Speciation Analysis by IC-ICP-CRC-MS Each sample for selenium speciation 

analysis was analyzed by ion chromatography inductively coupled plasma collision reaction 

cell mass spectrometry (IC-ICP-CRC-MS) on July 18, 2012. An aliquot of each sample is

injected onto an anion exchange column and mobilized by a basic (pH > 7) gradient. The 

eluting selenium species are then introduced into a radio frequency (RF) plasma where 

energy-transfer processes cause desolvation, atomization, and ionization. The ions are 

extracted from the plasma through a differentially-pumped vacuum interface and travel 

through a pressurized chamber (CRC) containing a reaction gas which preferentially reacts 

with interfering ions of the same target mass to charge ratios (m/z). A solid-state detector 

detects ions transmitted through the mass analyzer and the resulting current is processed by a 

data handling system. 

Retention times for each eluting species are compared to known standards for species 

identification. 

4. Analytical Issues 

The overall analyses went well and no significant analytical issues were encountered. All 

quality control parameters associated with the samples were within acceptance limits. 

The estimated method detection limits (eMDLs) for selenite, selenate, and selenocyanate are 

generated from replicate analyses of the lowest standard in the calibration curve. Not all 

selenium species are present in preparation blanks; therefore, eMDL calculations based on 

preparation blanks are artificially biased low. 

The eMDL for methylseleninic acid and selenomethionine is calculated from the average 

eMDL of selenite, selenate, and selenocyanate. The calibration does not contain 

methylseleninic acid or selenomethionine due to impurities in these standards which would 

bias the results for other selenium species. 

Page 42 of 48
If you have any questions or concerns regarding this report, please feel free to contact me. 

Sincerely, 

Russell Gerads 

Vice President 

Applied Speciation and Consulting, LLC 

Page 43 of 48
Sample Results

Sample ID Se(IV) Se(VI) SeCN MeSe(IV) SeMe

Unknown Se 

Species (n)

FGD Purge Eff ND (<2.2) 723 ND (<1.2) ND (<1.6) ND (<1.6) 0 (0)

BioReactor 1 Inf 10.3 487 ND (<0.31) 1.02 ND (<0.40) 0.45 (1)

BioReactor 2 Inf 19.6 192 ND (<0.31) 0.75 ND (<0.40) 0 (0)

BioReactor 2 Eff ND (<0.56) ND (<0.33) ND (<0.31) ND (<0.40) ND (<0.40) 0 (0)

All results reflect the applied dilution and are reported in μg/L

ND = Not detected at the applied dilution

SeCN = Selenocyanate

MeSe(IV) = Methylseleninic acid

SeMe = Selenomethionine

Unknown Se Species = Total concentration of all unknown Se species observed by IC-ICP-MS

n = number of unknown Se species observed

Report Generated by: Russell Gerads

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: Allen Shay/MillerCreek

Contact: Jay Perkins

Date: July 25, 2012

LIMS #J12070244

Page 44 of 48
Quality Control Summary - Preparation Blank Summary

Analyte (μg/L) PBW1 PBW2 PBW3 PBW4 Mean StdDev eMDL* eMDL 250x eMDL 1000x

Se(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.0022 0.56 2.2

Se(VI) 0.000 0.000 0.000 0.000 0.000 0.000 0.0013 0.33 1.3

SeCN 0.000 0.000 0.000 0.000 0.000 0.000 0.0012 0.31 1.2

MeSe(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.0016 0.40 1.6

SeMe 0.000 0.000 0.000 0.000 0.000 0.000 0.0016 0.40 1.6

eMDL = Estimated Method Detection Limit

*Please see narrative regarding eMDL calculations

Quality Control Summary - Certified Reference Materials

Analyte (μg/L) CRM True Value Result Recovery

Se(IV) LCS 9.57 9.37 97.9

Se(VI) LCS 9.48 9.09 95.9

SeCN LCS 8.92 8.82 98.9

MeSe(IV) LCS 6.47 6.78 104.7

SeMe LCS 9.32 8.15 87.4

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: Allen Shay/MillerCreek

Contact: Jay Perkins

LIMS #J12070244

Date: July 25, 2012

Report Generated by: Russell Gerads

Page 45 of 48
Quality Control Summary - Matrix Duplicates

Analyte (μg/L) Sample ID Rep 1 Rep 2 Mean RPD

Se(IV) Batch QC 47.4 48.0 47.7 1.4

Se(VI) Batch QC 22.8 20.7 21.7 9.7

SeCN Batch QC ND (<1.2) ND (<1.2) NC NC

MeSe(IV) Batch QC 2.4 2.4 2.4 1.8

SeMe Batch QC ND (<1.6) ND (<1.6) NC NC

ND = Not detected at the applied dilution

NC = Value was not calculated due to one or more concentrations below the eMDL

Quality Control Summary - Matrix Spike/ Matrix Spike Duplicate

Analyte (μg/L) Sample ID Spike Conc MS Result Recovery Spike Conc MSD Result Recovery RPD

Se(IV) Batch QC 5560 6427 114.7 5560 6463 115.4 0.6

Se(VI) Batch QC 5045 5465 107.9 5045 5522 109.0 1.0

SeCN Batch QC 4575 4586 100.2 4575 4599 100.5 0.3

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: Allen Shay/MillerCreek

Contact: Jay Perkins

LIMS #J12070244

Date: July 25, 2012

Report Generated by: Russell Gerads

Page 46 of 48
Page 47 of 48
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