# Scientific Assessment of Hypoxia in U.S. Coastal Waters

Interagency Working Group on Harmful Algal Blooms, Hypoxia, and Human Health September 2010 Dissolved oxygen (mg/L)

SalinityDepth (m) 

### 6 0

32 34 0

80

### This document should be cited as follows: Committee on Environment and Natural Resources. 2010. Scientific Assessment of Hypoxia in U.S. Coastal Waters. Interagency Working Group on Harmful Algal Blooms, Hypoxia, and Human Health of the Joint Subcommittee on Ocean Science and Technology. Washington, DC.



# Acknowledgements:

Many scientists and managers from Federal and state agencies, universities, and research institutions contributed to the knowledge base upon which this assessment depends. Man y thanks to all who contributed to this report, and special thanks to John Wickham and L ynn Dancy of NOAA National Centers for Coastal Ocean Science for their editing work.



## Cover and Sidebar Photos:
Background Cover and Sidebar: MODIS satellite image courtesy of the Ocean Biology Processing Group, NASA Goddard Space Flight Center. Cover inset photos from top: 1) CTD rosette, EPA Gulf Ecology Division; 2) CTD profile taken off the Washington coast, project funded by Bonneville Power Administration and NOAA Fisheries; Joseph Fisher, OSU, was chief scientist on the FV Frosti; data were processed and provided by Cheryl Morgan, OSU); 3) Dead fish, Christopher Deacutis, Rhode Island Department of Environmental Management; 4) Shrimp boat, EPA.

### Scientific Assessment of Hypoxia in U.S. Coastal Waters i

### Scientific Assessment of Hypoxia in U.S. Coastal Waters ii 

### This report is dedicated to the memory of Dr. Peter Eldridge, who was a member of the hypoxia report writing team and a research scientist with the U.S. Environmental Protection Agency. Peter had a great love and passion for the ocean, the environment, and science. Among Peter’s scientific contributions was the development of ecosystem models to address coastal environmental issues, such as coastal hypoxia, food web changes, and seagrass loss. Peter’s friendship and enthusiasm for science will be greatly missed. 

# Peter Eldridge (1946 – 2008)

### Scientific Assessment of Hypoxia in U.S. Coastal Waters iii Arctic Research Commission John Farrell
Department of Agriculture Louie Tupas
Department of Commerce National Oceanic and Atmospheric Administration Craig McLean Steve Murawski Roger Parsons
Department of Defense U.S. Army Corps of Engineers Charles Chesnutt Joan Pope
Department of Defense Office of Naval Research Linwood Vincent James Eckman
Department of Energy Office of Science Julie Carruthers James Ahlgrimm
Department of Health and Human Services Centers for Disease Control and Prevention Lorraine Backer G. David Williamson
Department of Health and Human Services Food and Drug Administration Robert Dickey William Jones
Department of Health and Human Services National Institutes of Health Allen Dearry
Department of Homeland Security U.S. Coast Guard Jonathan Berkson
Department of the Interior Kameran Onley Tim Petty
Department of the Interior Minerals Management Service James Kendall Walter Johnson Department of the Interior United States Geological Survey John Haines Linda Gunderson Department of Justice Matt Leopold Amber Blaha Department of State David Balton Liz Tirpak
Department of Transportation Maritime Administration Richard Corley U.S. Environmental Protection Agency Kevin Teichman Steven Hedtke

Executive Office of the President Council on Environmental Quality Hardy Pearce

Executive Office of the President Domestic Policy Council Paul Skoczylas

Executive Office of the President Office of Management and Budget Stuart Levenbach Kimberly Miller

Executive Office of the President Office of Science and Technology Policy Jerry Miller

Joint Chiefs of Staff Robert Winokur Nadeem Ahmad National Aeronautics and Space Administration Jack Kaye Eric Lindstrom

National Science Foundation Tim Killeen Julie Morris Phil Taylor

Marine Mammal Commission Robert Gisiner Tim Ragen

Smithsonian Institution Leonard Hirsch 

# Joint Subcommittee on Ocean Science and Technology (JSOST)

Steve Murawski, DOC/NOAA, Co-Chair Tim Killeen, NSF, Co-Chair Jerry Miller, OSTP, Co-Chair

### Scientific Assessment of Hypoxia in U.S. Coastal Waters iv Lorraine C. Backer (Co-Chair) Centers for Disease Control and Prevention

Paul A. Sandifer (Co-Chair) National Oceanic and Atmospheric Administration
Paula Bontempi Alternate: Fredric Lipschultz National Aeronautics and Space Administration

Herbert T. Buxton United States Geological Survey

David Garrison National Science Foundation
Rob Magnien Alternate: Quay Dortch National Oceanic and Atmospheric Administration

Steven Plakas U.S. Food and Drug Administration
Tim Ragen Alternate: Bob Gisiner Marine Mammal Commission

Teri Rowles National Oceanic and Atmospheric Administration
Joyce Saltsman U.S. Food and Drug Administration Juli Trtanj National Oceanic and Atmospheric Administration
Frederick L. Tyson National Institute of Environmental Health Sciences

Usha Varanasi Alternate: Walton Dickhoff National Oceanic and Atmospheric Administration

William Russo U.S. Environmental Protection Agency
Mark Walbridge Department of Agriculture



## Scientific support staff :

Elizabeth B. Jewett Cary B. Lopez Carolyn Sotka Virginia Fay National Oceanic and Atmospheric Administration
Cheryl L. Fossani National Science Foundation 

# JSOST Interagency Working Group on Harmful Algal Blooms, Hypoxia and Human Health (IWG-4H)

Primary Authors Elizabeth B. Jewett, Cary B. Lopez, David M. Kidwell, Suzanne B. Bricker National Oceanic and Atmospheric Administration
Marianne K. Burke , Mark R. Walbridge U.S. Department of Agriculture Peter M. Eldridge , Richard M. Greene, James D. Hagy III U.S. Environmental Protection Agency

Herbert T. Buxton U.S. Geological Survey

Robert J. Diaz Virginia Institute of Marine Science



## Major Contributors

Cheryl Brown U.S. Environmental Protection Agency

Bill Peterson National Oceanic and Atmospheric Administration Jay Peterson and Cheryl Morgan Oregon State University

### Scientific Assessment of Hypoxia in U.S. Coastal Waters v 

# Table of Contents

vi List of Figures

vi List of T ables

vii List of Bo xes
vii List of Case Studies

viii List of Acron yms
1 Ex ecutive Summary

7 Chapter 1. Legislativ e Background, Report Overview and Development Process

11 Chapter 2. Causes and Status of Hypo xia in U.S. Coastal Waters

25 Chapter 3. F ederal Hypoxia and Watershed Science Research: Status and Accomplishments

47 Chapter 4. Future R esearch Directions and Interagency Coordination

57 R eferences

68 Appendices

69 Appendix I. F ederal Agency Hypoxia or Hypoxia-related Research

83 Appendix II. Geogr aphic Case Studies

118 Appendix III. T able of U.S. Systems Impacted by Hypoxia

### Scientific Assessment of Hypoxia in U.S. Coastal Waters vi Page #

12 Figure 1. Global distribution of systems af fected by low dissolved oxygen.

14 F igure 2. Change in number of U.S. coastal areas experiencing hypoxia from 12 documented areas in 1960 to over 300 now

.
15 Figure 3. Comparison of the relative contribution of major sources of nitrogen pollution in three coastal ecosystems experiencing hypoxia.
16	 Figure 4. Conceptual diagram illustrating development and effects of hypoxia in stratified waters.

18 Figure 5. The range of ecological impacts exhibited as dissolved oxygen levels drop from saturation to anoxia.
19	 Figure 6. Conceptual view of how hypoxia alters ecosystem energy flow with example systems.

23 Figure 7. Relative magnitude and contribution of land management practices versus climate change factors to expansion or contraction of low dissolved oxygen.
25	 Figure 8. Conceptual diagram explaining how, in an adaptive management framework, scientific research informs management of environmental problems such as hypoxia.

27 Figure 9. Schematic describing general areas of hypoxia-related research.

29 Figure 10. Hypoxia is most intensively monitored in the largest and most impacted coastal systems in the United States. Examples include: a) Long Island Sound, b) Chesapeake Bay , c) Lake Erie, and d) Northern Gulf of Mexico.

31 Figure 1 1. Ensemble forecasts of the response of hypoxia to changes in riverine nitrogen load.

33 Figure 12. Trends in catch per unit effort for brown shrimp in the northern Gulf of Mexico.

34 Figure 13. Relationship between annual landings of brown shrimp in the northern Gulf of Mexico and the size of the hypoxic zone.
35	 Figure 14. Map of Gulf of Mexico with darker shaded areas indicating denser fish populations in the1960s.

37 Figure 15. Estimated nitrate delivery to the Gulf of Mexico for April, May, and June in1979 - 2008.
40	 Figure 16. Percent of the United States drained by artificial means such as tile drains.

42 Figure 17. 2007 Map of CEAP projects.

83 Figure A1. Geographic locations of hypoxia case studies. 

# List of Figures

**List of Tables**

Page #

13 T able 1. Percentage of U.S. estuaries and coastal water bodies with reports of hypoxia.

20 T able 2. Principal ecosystem characteristics and services impacted by hypoxia.

21 T able 3. Examples of hypoxia-related economic impacts.

22	 Table 4. Estimated influence of climate drivers on the extent and severity of hypoxia.

30 T able 5. Models developed since 2002 to forecast or simulate Gulf of Mexico hypoxia.

84 T able A1. Comparison of Physical Systems Represented by Case Studies in Appendix II.

### Scientific Assessment of Hypoxia in U.S. Coastal Waters vii Page #

7 Box 1. HABHRCA 2004 Reports and Assessments

8 Box 2. Legislation Relevant to Hypoxia

11	 Box 3. Hypoxia Definition

26 Box 4. Adaptive Management Approach for Gulf of Mexico Hypoxic Zone

27 Box 5. Adaptive Management Approach for Chesapeake Bay

28	 Box 6. Sound Science Leads to Significant Reductions in Hypoxia in Long Island Sound

29 Box 7. Hypoxia Advanced Warning Protects Drinking Water

38 Box 8. Application of SPARROW for Reducing Nutrients to the Gulf of Mexico

39 Box 9. EP A Works Closely With States to Develop and Adopt Nutrient Criteria

41 Box 10. Soil Drainage Research in Ohio

43 Box 1 1. Monitoring Winter Cover Crop Performance from Space

43	 Box 12. USDA Conservation Research Program Benefits in the Mississippi River Basin

54 Box 13. USGS Deployment of New Instruments to Measure W ater Flow and Sediment Flux 

# List of Boxes

Page #

85 Long Island Sound

89 Lake Erie

92 Chesapeake Bay

99 Pensacola Bay

102 Northern Gulf of Mexico

108	 Northeast Pacific Continental Shelf

112 Y aquina Bay

116 Hood Canal 

# List of Case Studies

### Scientific Assessment of Hypoxia in U.S. Coastal Waters viii ADMS Agricultural Drainage Management Systems
ARS Agricultural Research Service, USDA
BMP best management practice
CBEMP Chesapeake Bay Environmental Model Package
CEAP Conservation Effects Assessment Project
CENR Committee on Environment and Natural Resources
CHRP Coastal Hypoxia Research Program, NOAA
CRP Conservation Reserve Program, USDA
CSREES Cooperative State Research, Education, and Extension Service, USDA
DOD U.S. Department of Defense
DOE U.S. Department of Energy
DOI U.S. Department of the Interior
EISA Energy Independence and Security Act of 2007
EMAP Environmental Monitoring and Assessment Program
EMVL Environmental Modeling and Visualization Laboratory
EPA U.S. Environmental Protection Agency
ERS Economic Research Service, USDA
FSA Farm Service Agency, USDA
FVCOM Finite Volume Community Ocean Model
GLNPO Great Lakes National Program Office, EPA
HAB harmful algal bloom
HABHRCA Harmful Algal Bloom and Hypoxia Research and Control Act
HCDOP Hood Canal Dissolved Oxygen Program
IFYLE International Field Years on Lake Erie
IOOS Integrated Ocean Observing System
IWG-4H Interagency Working Group on HABs, Hypoxia, and Human Health
LaMP Lakewide Management Plan, Lake Erie
LISS Long Island Sound Study
LMAV Lower Mississippi Alluvial Valley
LUMCON Louisiana Universities Marine Consortium
MARB Mississippi Atchafalaya River Basin
MDA Maryland Department of Agriculture
NASA National Aeronautics and Space Administration NAWQA National Water-Quality Assessment Program, USGS
NBEP Narragansett Bay Estuary Program
NCER National Center for Environmental Research, EPA
NCII Nutrient Control Implementation Initiative NEP National Estuary Program, EPA
NERL National Exposure Research Laboratory, EPA
NERRS National Estuarine Research Reserve System, NOAA
NHEERL National Health and Environment Effects Research Laboratory, EPA
NMFS National Marine Fisheries Service, NOAA
NOAA National Oceanic and Atmospheric Administration, U.S. Department of Commerce
NRC National Research Council
NRCS Natural Resources Conservation Service, USDA
NRL Naval Research Laboratory, U.S. Department of Defense
NRMRL National Risk Management Research Laboratory, EPA
NSF National Science Foundation
OST Office of Science and T echnology, EPA
OW Office of Water, EPA
OWOW Office of Wetlands, Oceans, and Watersheds, EPA
PDO model Nitrogen-Phytoplankton-Detritus-Oxygen model
ReCON Real-time Coastal Observation Network
REMM Riparian Ecosystem Management Model
ReNuMa Regional Nutrient Management Model
ROMS Regional Ocean Model System RZWQM Root Zone Water Quality Model
SAV submerged aquatic vegetation
SEAMAP Southeast Area Monitoring and Assessment Program, NOAA
SPARROW SPAtially Referenced Regressions On Watershed Attributes
STAR Science to Achieve Results Program, EPA
STEWARDS Sustaining the Earth’s Watersheds Through Research, Data Analysis, and Synthesis
STORET STOrage and RETrieval data system, EPA
SWAT Soil and Water Assessment Tool
SWMP System-Wide Monitoring Program
SWWRP System-Wide Water Resources Program
TMDL total maximum daily load
USACE U.S. Army Corps of Engineers, DOD
USDA U.S. Department of Agriculture
USFWS U.S. Fish and Wildlife Service, DOI
USGS U.S. Geological Survey, DOI
WRSIS Wetland Reservoir Subirrigation System 

# List of Acronyms

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 1 

# The Problem 

# T he occurrence of hypoxia, or low dissolved oxygen, is increasing in coastal waters worldwide and represents a significant threat to the health and economy of our Nation’s coasts and Great Lakes. This trend is exemplified most dramatically off the coast of Louisiana andTexas, where the second largest eutrophicationrelated hypoxic zone in the world is associated with the nutrient pollutant load discharged by the Mississippi and Atchafalaya Rivers. Aquatic organisms require adequate dissolved oxygen to survive. The term “dead zone” is often used in reference to the absence of life (other than bacteria) from habitats that are devoid of oxygen. The inability to escape low oxygen areas makes immobile species, such as oysters and mussels, particularly vulnerable to hypoxia. These organisms can become stressed and may die due to hypoxia, resulting in significant impacts on marine food webs and the economy. Mobile organisms can flee the affected area when dissolved oxygen becomes too low. Nevertheless, fish kills can result from hypoxia, especially when the concentration of dissolved oxygen drops rapidly. New research is clarifying when hypoxia will cause fish kills as opposed to triggering avoidance behavior by fish. Further, new studies are better illustrating how habitat loss associated with hypoxia avoidance can impose ecological and economic costs, such as reduced growth in commercially harvested species and loss of biodiversity, habitat, and biomass. Transient or “diel-cycling” hypoxia, where conditions cycle from supersaturation of oxygen late in the afternoon to hypoxia or anoxia near dawn, most often occurs in shallow, eutrophic systems (e.g., nursery ground habitats) and may have pervasive impacts on living resources because of both its location and frequency of occurrence. Although coastal hypoxia can be caused by natural processes, a dramatic increase in the number of U.S. waters developing hypoxia is linked to eutrophication due to nutrient (nitrogen and phosphorus) and organic matter enrichment resulting from human activities. Sources of enrichment include point source discharges of wastewaters, nonpoint source atmospheric deposition, and nonpoint source runoff from croplands, lands used for animal agriculture, and urban and suburban areas. The incidence of hypoxia has increased ten-fold globally in the past 50 years and almost thirty-fold in the United States since 1960, with more than 300 systems recently experiencing hypoxia (Diaz & Rosenberg 2008; Table 1 and Appendix III). Diffuse runoff from nonpoint sources, such as agriculture fields, can be difficult to control, although improved production methods that reduce tillage, optimize fertilizer application, and buffer fields from waterways can mitigate water quality impairments. Despite the use of improved production methods in recent years, agriculture is still a leading source of nutrient pollution in many watersheds due, in part, to the high demand for nitrogen-intensive crops, principally corn. Furthermore, drainage practices, including tile drainage, have brought wetlands into crop production, short-circuited pathways (such as denitrification) that could ameliorate nutrient loading, and increased the transport of nitrogen into waterways. Atmospheric nitrogen deposition due to fossil fuel combustion has declined in many areas due to emission controls, but it remains an important source of diffuse nutrient loading. The difficulty of reducing nutrient inputs to coastal waters results from the close association between nutrient loading and a broad array of human activities in watersheds and explains the growth in the number and size of hypoxic zones. Unfortunately, hypoxia is not the only stressor impacting coastal ecosystems. Overfishing, harmful algal blooms (HABs), toxic contaminants, and physical alteration of coastal habitats associated with coastal development are several problems that co-occur with hypoxia and interact to decrease the ecological health of coastal waters and reduce the ecological services that they can provide. 

# Executive **Summary**

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 2 

## Executive Summary



# Legislative Mandates for Action The Harmful Algal Bloom and Hypoxia Research and Control Act (HABHRCA) mandated creation of this report, which serves as a thorough update to the first scientific assessment of hypoxia released in 2003. Several other legislative drivers also influence how Federal agencies work on coastal water quality including the Clean Water Act; the Food, Conservation, and Energy Act of 2008 (“Farm Bill”); the Energy Independence and Security Act of 2007; and the Coastal Zone Management Act. Responsibility for resolving hypoxia spans several Federal agencies (U.S. Department of Agriculture, U.S. Geological Survey, U.S. Environmental Protection Agency, and National Oceanic and Atmospheric Administration), which oversee research and management/control programs (Appendix I). States play a critical role in monitoring and managing hypoxia, but their efforts are not addressed in detail here because this report was mandated to focus on Federal efforts. 

# Adaptive Management
Adaptive management recognizes that science should inform management decision-making, not only as the original questions are posed but as scientific understanding continues to develop. Current scientific understanding of the factors contributing to hypoxia is derived from rigorous research that Federal agencies have been conducting and funding for at least 25 years. Federal hypoxia research has made significant progress in describing and quantifying the causes of hypoxia and reducing uncertainties required to confidently proceed with an adaptive management approach. For example, the ecological mechanisms linking nutrient loading to hypoxia in the Chesapeake Bay were quantified sufficiently to justify an initial 40% nutrient reduction goal in the mid-1980s; further research and sophistication in simulation models have been used subsequently to support three additional rounds of adaptive management leading to more detailed nutrient reduction strategies. From a national perspective, adaptive management approaches will have to be flexible enough to address differences among ecosystems (e.g., geography, level of watershed development, agricultural influence, nutrient loading, physical circulation of the waterbody) and future contingencies as they unfold. For example, it is likely that climate change will affect the incidence of hypoxia in coastal waters. A flexible management approach will enable response to climate impacts as they are realized. A flexible and meaningful adaptive management strategy for agricultural production―including biofuels― should be explored in order to ensure that agricultural products are produced in a manner that minimizes or prevents water quality impairments. Reducing hypoxia via nutrient loading reductions will require a concerted effort by a diverse group of stakeholders. Implementing these actions will likely require organized programs to monitor, study, and manage water quality problems. Such programs have already been established for a few coastal waterbodies (e.g., Lake Erie, Gulf of Mexico, Chesapeake Bay, Long Island Sound). In the case of other waterbodies where the problem has just recently been recognized and understood (e.g., Narragansett Bay, Rhode Island; Pensacola Bay, Florida), only nascent efforts, if any, are in place to address it.

Scientists use the box corer to sample sediments with negligible disturbance. Sediment cores are used to study sediment-oxygen-nutrient dynamics. Photo. L.Jewett, NOAA

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 3 

## Executive Summary



# Federal Research Accomplishments and Opportunities for Advancement
Understanding and managing hypoxia requires research and management actions to address entire watersheds and their coastal receiving waters. This report examines progress in: 1) understanding the dynamics of hypoxia where it occurs (i.e., in estuaries, coastal waters, and the Great Lakes); 2) understanding and monitoring nutrient fluxes in watersheds; and 3) understanding how to reduce nutrient transport across the landscape. Because responsibility for management of nutrient enrichment in watersheds is shared across several Federal agencies, coordination and information-sharing are critical. Presently, interagency collaboration is most extensive and effective in places where investments in scientific and management activities have been largest and sustained for the longest period of time (e.g., Chesapeake Bay). Responsibility for monitoring dissolved oxygen in most coastal and Great Lakes waterbodies lies with states or with Federal-state partnerships that utilize state monitoring programs to track water quality. For many coastal and Great Lakes waters where hypoxia occurs, fairly rigorous methods have been implemented for measuring dissolved oxygen and conveying this information to scientists and the public. However, the two largest hypoxic zones in the United States, located at the mouth of the Mississippi River and on the Oregon continental shelf, occur beyond the limits of state waters and, thus, rely on Federal support for monitoring. Monitoring informs coastal managers about water quality conditions in the ecosystems they oversee, and it helps support the development and verification of ecosystem simulation models used to guide management decisions. Since the last scientific assessment of hypoxia was written in 2003, many computer models have been developed or updated to simulate ecological processes related to hypoxia in estuarine, coastal, and Great Lakes ecosystems and their watersheds. Sophisticated physical transport models, which examine ocean currents and mixing, are increasingly being coupled with water quality models to examine alternatives for managing hypoxia. These models are most effective when they reflect a strong scientific understanding of the processes that control hypoxia development. Relatively simple regression models can also be effective for testing hypotheses regarding factorsthat control hypoxia, especially as more longterm data on hypoxia become available. The relative simplicity of regression analyses has also made them useful tools for short-term ecological forecasting. Regression analyses have been used for annual hypoxia forecasts for the northern Gulf of Mexico and Chesapeake Bay. Ecological box models, which simplify processes into broader geographic resolution, have improved greatly and have begun to incorporate bioeconomic components for assessing impacts of hypoxia on commercial and recreational fisheries. Advances have been particularly dramatic in watershed science. Watershed models now allow scientists to quantify the contribution of particular geographic regions to nutrients entering a coastal system. A suite of models connecting sources of nutrients in watersheds to the development of hypoxia in receiving waters, ideally with quantified uncertainty, is needed by resource managers to Researchers retrieve the CTD rosette, used for collecting water and measuring dissolved oxygen and other oceanographic parameters, after a sampling profile is completed during rough seas. Photo: L.Jewett, NOAA

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 4 

## Executive Summary

justify potentially costly actions to reduce nutrients and hypoxia. Use of a multiple model consensus, where models use different approaches and assumptions, provides one way to better inform management decisions. This approach is well known for its application to forecasting hurricane paths and predicting the effects of carbon dioxide fluctuations on climate. Monitoring stream and river flow and the associated nutrients is critically important for evaluating nutrient sources in the watershed and modeling fluxes to coastal waterbodies. Stream and river monitoring was made more efficient, maximizing the use of existing resources, through the redesign of the National Stream Quality Accounting Network in 2007. However, it is important not only to maintain the current streamflow gauges and water quality monitoring stations, but also to increase the number of streams monitored. Similar to hypoxia monitoring, the monitoring of nutrients in rivers and streams is required for the development and use of predictive models and to determine the cause-effect relationships between activities that alter water nutrient export and the changes in water quality in the receiving water. Expanded stream monitoring, in combination with modeling efforts, will allow design of effective nutrient reduction strategies and will improve the ability to track progress toward water quality goals. Thus, effective and sustained management of hypoxia will require long-term support of stream and river water quality monitoring programs. Research exploring hypoxia impacts on fish and invertebrates has shown negative effects on growth, reproduction, species composition, and ecological interactions among species. However, scaling up these impacts from individuals to entire fisheries populations or ecosystems is extremely complex and requires sophisticated ecosystemmodels that are generally beyond the state-ofthe-science. Thus, research should be focused on developing these models and linking them to those that include more conventional water quality components. An additional modeling challenge will be resolving the impacts of hypoxia on living resources in systems affected by a suite of stressors as we move toward more comprehensive ecosystem management approaches. Reducing nutrients from anthropogenic sources can lead to a reduction in coastal hypoxia and improvements in water quality throughout the watershed. Nutrient loads in some systems, such as Long Island Sound and Narragansett Bay, are being successfully reduced through targeting of wastewater treatment plants. However, nutrient loads from municipal and industrial facilities, defined as point sources, are more easily addressed than the diffuse sources of nutrients entering waterways as runoff from agricultural and urban lands. Significant Federal investments and scientific effort have focused on developing efficient and effective ways to reduce nutrient runoff, including land conservation programs (which provide incentives for farmers to take marginal agricultural land out of production, invest in working landsto reduce erosion and control nutrients, and reestablish wetlands), alternative drainage systems, remote sensing to target fertilizer applications, winter cover crops, and newly formulated fertilizers. Unfortunately, measures implemented on nonpoint sources to date have proven ineffective at reducing nutrient loads from large watersheds to levels needed to significantly reduce hypoxic zones. For example, reductions in total spring nitrogen loads to the Gulf of Mexico from 2001- 2005 were primarily from forms of nitrogen other than nitrate, which is a critical form fueling spring primary production leading to hypoxia. This highlights the importance of the nutrient composition, as well as seasonality, for reduction targets. Further, evidence suggests that some ecosystems may not respond as expected to nutrient management as they become nutrient saturated. Thus, major new initiatives should include rigorous scientific evaluation of the effectiveness of existing nutrient reduction strategies through local analyses of downstream water quality so that strategies can be refined accordingly. Continued research is also needed on new practices, such as those that enhance natural nutrient processes through

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 5 

## Executive Summary

reforestation, river diversions into wetlands, and vegetation buffer systems for streams and rivers. Such research has already led to management decisions to protect wetlands and to reforest portions of watersheds. Establishment of criteria for acceptable nutrient levels in both coastal areas and in rivers upstream from hypoxic zones is also a critical tool for designing management plans. These criteria can also be used to measure progress. 

# Conclusion
Hypoxia is a major contributor to the decline of coastal water quality observed in recent decades, and its extent has been expanding. It is part of the broader issue of nutrient-driven eutrophication. Eutrophication is also linked to increased HABs, loss of seagrasses, and other impacts on coastal ecosystems. For eutrophic ecosystems, concerted and coupled research and management efforts, along with stakeholder support, will be needed to rigorously identify, quantify, and implement nutrient reduction strategies that are effective and achievable. Furthermore, for systems such as the Oregon shelf where hypoxia is driven primarily by natural processes linked to variations in climate, improved scientific understanding will provide insight into future impacts of climate change on similar ecosystems. Moreover, knowledge gained will be important for developing forecasts of the extent and severity of low dissolved oxygen, which will help managers mitigate the impacts of hypoxia. If properly planned and executed, adaptive management of nutrient inputs will lead to significant reductions in hypoxia. However, if current practices are continued, the expansion of hypoxia in coastal waters will continue and increase in severity, leading to further impacts on marine habitats, living resources, economies, and coastal communities.

Sunset aboard EPA’s Ocean Survey Vessel Peter W. Anderson during a research cruise in the Gulf of Mexico hypoxic zone. Photo: EPA

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 7 

# Legislative **Background, Report Overview, and Development Process**  Chapter 1

1.1. Legislative Background
In the early 1980s, concern about low dissolved oxygen in coastal waterbodies of the United States led to the first national assessment of coastal hypoxia (Whitledge 1985), which found dissolved oxygen levels in many U.S. waterbodies on the decline as a result of eutrophication. By the 1990s, serious and large-scale water quality problems were identified, including harmful algal blooms (HABs) and hypoxia, most prominently in the northern Gulf of Mexico, Lake Erie, Chesapeake Bay, and Long Island Sound. These problems led to a national assessment of eutrophication in 1999 (Bricker et al. 1999), which was subsequently updated (Bricker et al. 2007), and an integrated assessment of hypoxia in the northern Gulf of Mexico (CENR 2000, U.S. EPA 2007), as well as the passage of the Harmful Algal Bloom and Hypoxia Research and Control Act of 1998 (HABHRCA, Public Law 105-383). HABHRCA was reauthorized by the Harmful Algal Bloom and Hypoxia Amendments Act of 2004 (HABHRCA 2004, Public Law 108-456). HABHRCA 2004 reconstituted the Interagency Task Force on HABs and Hypoxia. To fulfill the requirements of both HABHRCA 2004 and the Oceans and Human Health Act of 2004, the Interagency Task Force on HABs and Hypoxia was incorporated into the Interagency Working Groupon HABs, Hypoxia, and Human Health (IWG4H, see page iii) of the Joint Subcommittee on Ocean Science and Technology. HABHRCA 2004 required five reports to assess and recommend research programs on HABs and hypoxia in U.S. waters, including this report, a Scientific Assessment of Hypoxia in U.S. Coastal Waters (Box 1). HABHRCA 2004 stipulates that this report should: • 

### examine the causes, ecological consequences, and economic costs of hypoxia;

• 

### describe the potential ecological and economic costs and benefits of possible actions for preventing, controlling, and mitigating hypoxia;

• 

### evaluate progress made by and needs of Federal research programs; and

• 

### identify ways to improve coordination among Federal agencies with respect to research on hypoxia. HABHRCA 2004 also authorizes appropriations to the Secretary of Commerce and the National Oceanic and Atmospheric Administration (NOAA) to conduct research on hypoxia. Additional legislation affecting Federal research on hypoxia or factors that may affect hypoxia is listed in Box 2. The Coastal Zone Act Reauthorization Amendments of 1990 reauthorized the Coastal Zone Management Act



## Box 1. HABHRCA 2004 Reports and Assessments

• 

### Harmful Algal Bloom Management and Response: Assessment and Plan

• National Assessment of Efforts to Predict and Respond to Harmful Algal Blooms in U.S. Waters (Prediction and Response Report)

• Report on National Scientific Research, Development, Demonstration, and Technology Transfer Plan for reducing HAB Impacts (RDDTT Plan)

• 

### Scientific Assessment of Freshwater Harmful Algal Blooms

• 

### Scientific Assessment of Marine Harmful Algal Blooms

• 

### Scientific Assessment of Hypoxia in U.S. Coastal Waters

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 8 

## Chapter 1

and established a joint program between the U.S. Environmental Protection Agency (EPA) and the states to develop and implement coastal nonpoint pollution management programs. We note that EPA’s Mississippi River Basin program will be enhanced in 2011, pending enactment of the President’s proposed budget. The Water Resources Development Act authorizes flood control, navigation, and environmental projects and studies by the U.S. Army Corps of Engineers (USACE), and it states that the Secretary of the Army may participate in assessments of hypoxia in the northern Gulf of Mexico. Some of this legislation focuses on water quality in upland watersheds, which ultimately may affect the quality of water delivered to coastal ecosystems; therefore, it also impacts coastal hypoxia. The Clean Water Act aims to restore and maintain the chemical, physical, and biological integrity of the Nation’s waters by reducing point and nonpoint pollution sources, providing assistance to publicly owned treatment works for improving wastewater treatment, and maintaining the integrity of wetlands. The Food, Conservation, and Energy Act of 2008 (the “Farm Bill”) authorized $25 billion to support conservation programs administered by the U.S. Department of Agriculture (USDA) through its Natural Resources Conservation Service (NRCS) and Farm Service Agency (FSA). Finally, the Energy Independence and Security Act (EISA) of 2007 potentially has far-reaching environmental implications. The bill mandates production of 36 billion gallons of biofuels by the year 2022, including 15 billion gallons of corn-based ethanol. The legislation designates the EPA, USDA, and U.S. Department of Energy (DOE) as lead agencies in an interagency effort to develop criteria for sustainable development of biofuels, which include prevention of water quality and ecosystem impairments. 

# 1.2. Report Overview

This assessment focuses on the science that forms the basis for improving knowledge of the causes, impacts, and solutions for hypoxia in coastal ecosystems of the United States as of 2009. This report has four chapters and three appendices:

• 

### Chapter 1: Legislative background, report overview, and report development process;

• 

### Chapter 2:The current status of hypoxia in U.S. coastal waters, the spectrum of causal factors contributing to hypoxia, and the ecological and economic consequences of hypoxia;

• 

### Chapter 3: Status and accomplishments of current Federal monitoring, assessment, and research activities related to hypoxia, including research on coastal ecosystems and their watersheds that provides the basis for effective management actions;

• 

### Chapter 4: Directions for future science activities and opportunities to increase effectiveness and cost-efficiency through coordination of programs across Federal agencies;

• 

### Appendix I: Federal Government Hypoxia or Hypoxia-related Research;

• 

### Appendix II: Case studies that feature coastal ecosystems across the United States affected by hypoxia. The featured systems were selected to illustrate the range of circumstances causing hypoxia as well as the range in status of scientific understanding and management approaches being implemented (the case studies are referenced throughout the report); and

• 

### Appendix III: Details on U.S. coastal systems affected by hypoxia. 

# 1.3. The Report Development Process
This report was prepared by a task force associated with the IWG-4H that included representatives of Federal agencies participating in the science and management of coastal hypoxia. 

## Box 2. Legislation Relevant to Hypoxia

• 

### Harmful Algal Bloom and Hypoxia Research and Control Act

• 

### Coastal Zone Management Act

• 

### Water Resources Development Act

• 

### Clean Water Act

• 

### Food, Conservation, and Energy Act of 2008

• 

### Energy Independence and Security Act of 2007

• 

### Annual appropriations of Federal agencies

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 9 

## Legislative Background and Report Process

It builds on earlier reports to assess hypoxia in U.S. coastal waters by updating the assessments and summarizing the major advances in hypoxia research during the past five years. Specifically, this report draws on An Assessment of Coastal Hypoxia and Eutrophication in U.S. Waters (CENR 2003), which was called for in HABHRCA 1998. This report also recommends priorities for future hypoxia-related research across the U.S. government.

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 11 

# 2.1. The Issue of Hypoxia 

# H ypoxia, or water with dissolved oxygen that is too low to support fish and other important species (Box 3), has been recognized as one of the most important water quality problems worldwide (Diaz and Rosenberg 2008). Hypoxia has become a serious problem along all of the Nation’s coasts and in the Great Lakes. Hypoxic areas are often termed “dead zones” since the only organisms that thrive there are those that can live with little or no oxygen, often only bacteria. Most mobile organisms are able to avoid hypoxic waters by swimming or crawling away; organisms unable to move or those trapped within a zone of hypoxia become physiologically stressed and die if exposure is prolonged or severe (Diaz and Rosenberg 1995, Vaquer-Sunyer and Duarte 2008). Most coastal hypoxia is associated at the global scale with either areas of high population density or developed watersheds that export large quantities of nutrients and organic matter (Figure 1, Rabalais et al. 2007, Galloway et al. 2008, Diaz and Rosenberg 2008). The number of waterbodies with recorded and published accounts of low dissolved oxygen from around the globe has increased about an order of magnitude during the last 50 years, from less than 50 in the 1960s to about 400 by 2008 (Diaz and Rosenberg 2008). The number of waterbodies in the United States with documented hypoxia followed the same trend, increasing from 12 prior 

# Causes and Status of Hypoxia **in U.S. Coastal Waters** Chapter 2

to 1960 to over 300 by 2008 (Appendix III; Figure 2). The second largest eutrophication-related hypoxic area in the world (after the Baltic Sea, which is approximately 80,000 square kilometers, or km 2 

### , Karlson et al. 2002, Hansen et al. 2007) occurs in the United States, and is associated with the discharge from the Mississippi/Atchafalaya Rivers in the northern Gulf of Mexico. The Gulf of Mexico hypoxic area is similar in extent and volume to another large hypoxic area associated with China’s Changjiang River outflow (13,700 km 2 

### in 1999, Li et al. 2002). The northern Gulf of Mexico hypoxic area has increased substantially in size since the mid-1980s when it was first measured at about 4,000 km 2 

### (Rabalais et al. 2007). In 2008, it encompassed 20,719 km 2 

### , the second largest area on record (http://www.gulfhypoxia.net/). A concerted Federal interagency and multi
## Box 3. Hypoxia Definition

Hypoxia means “low oxygen”. In aquatic and marine systems, low oxygen generally refers to a dissolved oxygen concentration less than 2 to 3 milligrams of oxygen per liter of water (mg/L), but sensitive organisms can be affected at higher thresholds (4.5 mg/L). A complete lack of oxygen is called anoxia. Hypoxic waters generally do not have enough oxygen to support fish and other aquatic animals, and are sometimes called dead zones because the only organisms that can live there are microbes. The criteria set for health of various species in Chesapeake Bay are a good example of how one definition for hypoxia is not possible (EPA 2003).

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 12 

## Chapter 2

state effort has been underway since 2000 to find ways to reduce the size of the hypoxic area (CENR 2000, U.S. EPA 2007; see Gulf of Mexico Case Study, Appendix II). Much of the scientific interest and management concern about hypoxia is focused on its principal
cause, eutrophication , which is defined as “an increase in the rate of supply of organic matter to an ecosystem” (Nixon 1995). Eutrophication is most often associated with nutrient enrichment of coastal waters from urban and agricultural land runoff, wastewater treatment plant discharges, and air deposition of nutrients (Bricker et al. 2007, Galloway et al. 2008) (see Section 2.3.2). The combination of stressors associated with eutrophication, which includes both HABs and hypoxia, continues to degrade U.S. coastal waters, estuaries, and the Great Lakes. Nutrient management, primarily via improved treatment of industrial and municipal point source discharges, has reduced hypoxia in some systems (e.g., Los Angeles Harbor, Delaware River, and Hillsborough Bay in Florida). However, a concerted and sustained effort to address nonpoint sources of nutrients will be needed to allow more waterbodies to recover and to protect our estuarine and coastal resources for the future. 

# 2.2. Status of Hypoxia in the United States
Hypoxia is a serious problem in estuarine and coastal areas of the United States resulting from a diverse set of causes, including eutrophication driven mainly by nonpoint sources of nutrients (Diaz and Rosenberg 2008). The present day status of hypoxia contrasts with that of fifty years ago when it was primarily a problem in rivers resulting from inadequate treatment of municipal and industrial wastes. Through the 1970s, organic matter loading from sewage and industrial discharges was the most salient cause of eutrophication and hypoxia in the United States (Smith et al. 1987). The majority of the 69 hypoxic U.S. waterbodies known from that time period were related to discharges from wastewater treatment (e.g., Providence River, Melrose et al. 2007) or industry (e.g., Pensacola Bay, Hagy and Murrell 2007). Actions following the passage of the Clean Water Act in 1972 reduced these point source organic loads and, much later, began to reduce point source phosphorus and (even later) nitrogen loading, contributing to some improvements in low dissolved oxygen problems. The Clean Water Act, however, did not explicitly address management and regulation of nonpoint

Figure 1. Global distribution of systems affected by low dissolved oxygen.

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 13 

## The Problem of Hypoxia in U.S. Waters

sources of pollutants. It was in the 1970s that the rising effects of nonpoint runoff of nutrients were observed in water quality deterioration around the United States (Smith et al. 1987). The 1970s were also when first reports of hypoxia appeared for our larger coastal systems (northern Gulf of Mexico, New York Bight, Long Island Sound). By the 1980s, it became apparent that increasing nutrient loads from nonpoint sources were responsible for an expansion of eutrophic conditions in estuarine and coastal systems within the United States (reviewed by Bricker et al. 1999, 2007). Overall, the United States has led a global trend in which agricultural fertilizers are the leading nonpoint source of nutrient pollution, but atmospheric deposition from burning of fossil fuels and nitrogen fixation associated with legume crops also contribute significantly (Howarth et al. 1996). The primary sources of nutrient pollution vary substantially among watersheds (e.g., Figure 3), complicating the task of nutrient management. A current analysis of 647 U.S. coastal and estuarine ecosystems indicates that the percentage of systems with hypoxia has increased dramatically since the 1960s (Figure 2) and even since the 1980s (Table 1). The first national assessment of oxygen conditions in U.S. waters, conducted in the 1980s, found 38% of systems to have hypoxia (Whitledge 1985). Subsequent eutrophication assessments found greater than 50% of systems to have hypoxia (Bricker et al. 1996, 1997a, 1997b, 1998a, 1998b, 2007). Updating the information from all these sources finds that 307 of 647 ecosystems have hypoxia (Appendix III). Most of the increases since the 1980s occurred in the North Atlantic, South Atlantic, and Pacific regions and reflect an increase in the incidence of hypoxia. In the Middle Atlantic and Gulf of Mexico regions, the percentage of hypoxic systems was already high in the 1980s and remains high to date. Further, the third National Coastal Condition Report (U.S. EPA 2008) found water quality for Atlantic and Gulf coasts to range from fair to poor, based on data from the early 2000s. For the Great Lakes, there appears to have been little change in hypoxic conditions since the 1980s. Dissolved oxygen conditions have improved in some waterbodies due to intensive regulation of nutrient or carbon inputs, primarily from more advanced sewage and industrial treatment. The best examples are from smaller systems where point source discharges were the primary source of organic matter and nutrients, such as the Hudson River in New York and the Delaware River in Pennsylvania and New Jersey (Brosnan and O’Shea, 1996; Patrick, 1988). In larger systems where point sources have been intensely managed, dissolved oxygen conditions have not improved because of large nonpoint sources of nutrients. Examples include Chesapeake Bay and Lake Erie, which have a long history of nutrient management but continuing problems with large-scale hypoxia. (See Appendix II). Even with improved management of nonpoint sources, recovery of some large systems may be delayed due to nutrient and organic matter that have accumulated in the sediments over the years, thereby increasing oxygen demand, and, in turn, expanding the amount of hypoxia for a given load of nutrients (Turner et al. 2008). This Region % Hypoxic 1980s 1 % Hypoxic 1990s 2 % Hypoxic 2000s 3 % Hypoxic 2000s 4
North Atlantic 6 (1 of 17) 22 (4 of 18) 35 (7 of 20) 26 (10 of 38)
Mid-Atlantic 50 (19 of 38) 59 (13 of 22) 64 (14 of 22) 42 (76 of 180)
South Atlantic 18 (9 of 51) 64 (14 of 22) 91 (20 of 22) 55 (77 of 139)
Gulf of Mexico 69 (38 of 55) 84 (32 of 38) 68 (26 of 38) 51 (105 of 205)
Pacific 21 (6 of 29) 26 (10 of 39) 35 (13 of 37) 46 (37 of 80)
Great Lakes 1 of 5 lakes 2 of 5 lakes 2 of 5 lakes
Total Nation 38 (73 of 190) 52 (73 of 139) 58 (80 of 139) 47 (307 of 647)

1 Based on Whitledge 1985a; 2 Based on Bricker et al. 1996, 1997a, 1997b, 1998a, 1998b; 3 Based on Bricker et al. 2007; 4 From Appendix III. 

### Table 1. Percentage of U.S. estuaries and coastal waterbodies with reports of hypoxia.

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 14 

## Chapter 2

may be the case in the northern Gulf of Mexico and Chesapeake Bay (Hagy et al. 2004, Turner et al. 2008). Unfortunately, nutrient loading from agricultural and atmospheric sources is predicted to increase globally over the next 50 years (Tilman et al. 2001, Galloway et al. 2008), creating additional stress on coastal waterbodies already in decline. Federal research and management actions must accelerate the pace of water quality management in order to protect and restore valued coastal aquatic

resources. 

# 2.3. Causes of Hypoxia
Widespread and persistent hypoxia is generally not a natural condition in estuaries, coastal waters, or large lakes like the Great Lakes, with Lake Erie being an exception (Appendix II). However, naturally occurring oxygen depletion does occur, as in oceanic oxygen minimum zones, fjords, some stratified seas (e.g., the Black Sea), lakes and ponds, and swamps and backwaters that circulate poorly and have large loads of natural land-derived organic matter. Naturally occurring hypoxia is not the focus of this report, but it is important to consider these cases because human activities may increase the frequency, duration, and intensity of naturally occurring hypoxia (Cooper and Brush 1991, Helly and Levin 2004, Diaz and Rosenberg 2008). When the rate of oxygen consumption in aquatic environments increases such that the oxygen consumption rate exceeds resupply, oxygen concentrations can quickly decline to levels insufficient to sustain most animal life. Two conditions are generally required for the development and maintenance of hypoxia (Figure 4):

• 

### water column stratification that isolates a layer of bottom water and sediments from a usually well-oxygenated surface layer (see Section 2.3.1), and

• 

### a source of organic matter, which is then decomposed and depletes oxygen in the isolated bottom layer (see Section 2.3.2). Diel-cycling hypoxia, where conditions can cycle from supersaturation of oxygen late in the afternoon to hypoxia or anoxia near dawn, is a unique situation that does not require the first condition described above. This phenomenon can occur in unstratified waters and appears to be increasing in frequency (D’Avanzo and Kremer 1994, Verity et al. 2006, Shen et al. 2008). It most often occurs in shallow, eutrophic tidal creeks where oxygen exchange at the water surface

Figure 2. Change in number of U.S. coastal areas experiencing hypoxia from 12 documented areas in 1960 to over 300 now (Appendix III). Not shown here are one hypoxic system in Alaska and one in Hawaii.

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 15 

## The Problem of Hypoxia in U.S. Waters

is insufficient to supply the respiratory needs of microbes and other organisms. This type of transient or diel hypoxia may cause pervasive impacts on living resources both because of the frequency with which it occurs (hours to days) and its location (small and shallow nursery ground habitats, e.g., Pepper Creek, Delaware; Tyler and Targett 2007, Tyler et al. 2008). The increased production of organic matter within a system, mostly in the form of phytoplankton productivity in response to anthropogenic nutrient pollution, contributes to both seasonal and diel-cycling hypoxia in coastal ecosystems. In shallow systems that are highly eutrophic, calm weather conditions and extended periods of cloud cover can cause low dissolved oxygen events (Tyler et al. 2008). Whereas physical factors create the physical conditions that are usually necessary for hypoxia to develop in bottom waters, nutrient-driven eutrophication provides the increased deposition of decomposing organic matter to the bottom layer, creating increased oxygen demand. Although physical factors can change over the long-term, they have not in most cases (e.g., Hagy et al. 2004, Greene et al. 2009). Thus, eutrophication is the principal cause of the long-term increases in hypoxia that have been observed. 2.3.1. Physical Factors Density stratification of the water column, in which a less dense layer of water floats on top of a denser bottom layer, is an almost universal characteristic of coastal systems subject to seasonal bottom water hypoxia. Stratification reduces the potential for oxygen from the atmosphere to replenish oxygen depleted at depth. In most cases involving marine systems, a vertical gradient of salinity, creating a halocline, is the most important factor contributing to density stratification. Surface heating, creating warmer surface water temperatures and thus a vertical gradient of temperature, or a thermocline, is the cause of stratification in lakes and can also contribute to density stratification in marine systems, especially during spring when deeper waters are relatively cold. Thermal stratification creates the potential for hypoxia in Lake Erie and is also known to be important in Long Island Sound and New York Bight (Boesch and Rabalais 1991, Hawley et al. 2005, Lee and Lwiza 2008). In the fall, cooling temperatures and decreasing freshwater inputs can destabilize summer stratification, leading to relatively abrupt mixing or “turnover” of the water column that eliminates seasonal hypoxia. Fall turnover is often associated with a wind event such as a storm or frontal passage. 

## Urban/Suburban Agriculture At mospheric 

# Chesapeake Bay

Urban/Suburban Agriculture Atmospheric

Urban/Suburban Agriculture

At mospheric 

# Narragansett Bay

Urban/Suburban Agriculture

Atmospheric

Agriculture Atmospheric

Urban/Suburban Pasture 

# Gulf of Mexico

Urba n/Suburban Pasture 

# Gulf of Mexico

Figure 3. Comparison of the relative contribution of major sources of nitrogen pollution in three coastal ecosystems experiencing hypoxia. Urban/suburban includes both point (industrial and sewage effluent) and nonpoint sources (residential run off). Data sources: Chesapeake Bay: Chesapeake Bay Program; Narragansett Bay: Nixon et al. 2008, Moore et al. 2004; Gulf of Mexico: Alexander et al. 2008. 

## Chesapeake Bay Narragansett Bay Gulf of Mexico

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 16 

## Chapter 2

In river-dominated estuaries and ocean margins, density stratification and other circulation features are strongly affected by the amount of less dense freshwater flowing out of associated rivers. Therefore, the extent and severity of seasonal hypoxia varies on an annual basis in relation to the magnitude of freshwater inputs. In larger systems, where freshwater is retained in the system for longer periods of time, summer hypoxia is usually coupled most closely to freshwater inputs during spring, when spring rains or snow melt cause high freshwater flow (e.g., Chesapeake Bay, Hagy et al. 2004; northern Gulf of Mexico, Greene et al. 2009). In smaller systems, physical conditions conducive to development of hypoxia may be coupled to freshwater inputs on a shorter time scale. For example, a single early fall pulse of freshwater associated with Hurricane Ivan stimulated development of an unseasonable period of hypoxia in Pensacola Bay, Florida, during fall 2004 (Hagy et al. 2006). Shen et al. (2008) observed periods of hypoxia lasting two to five days in a shallow mid-Atlantic creek following a rain event. Development and maintenance of hypoxia are strongly affected by water column mixing. Strong tidal mixing prevents water column stratification almost entirely in some systems. Where tides are not as strong, low dissolved oxygen events mayoccur during neap tides, the phase of the springneap tidal cycle when tidal mixing is minimal (Haas 1977). Stratification, and thus vulnerability to hypoxia, is reduced during the larger spring tides, which are associated with full and new moons, when tidal currents are stronger and generate increased mixing of the water column. Some estuaries, such as Mobile Bay, Alabama, Figure 4. Conceptual diagram illustrating development and effects of hypoxia in stratified waters (from Downing et al. 1999). The pycnocline is the boundary that stratifies and separates the bottom and surface water layers.

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 17 

## The Problem of Hypoxia in U.S. Waters

and Pensacola Bay, Florida, in the northeastern Gulf of Mexico have low amplitude tides (Hagy and Murrell 2007), and others, such as the Albermarle-Pamlico Estuarine System on the North Carolina coast, are virtually tideless (Luettich et al. 2002). These estuaries, which are also in a warmer climate that yields higher respiration and nutrient regeneration rates, are particularly susceptible to stratification and hypoxia. Wind is an especially important factor affecting hypoxia in these microtidal estuaries (Reynolds-Fleming and Luettich 2004, Park et al. 2007, Hagy and Murrell 2007).
2.3.2. Biological/chemical Factors Eutrophication , fundamentally a biological and biogeochemical process, is an important factor and indeed the principal cause of coastal hypoxia. In an update to the National Estuarine Eutrophication Assessment (Bricker et al. 2007, See Table I), eutrophication was recognized as a widespread problem, with 65% of assessed systems (99 of 141 systems had adequate data for analysis) showing moderate- to high-level problems. The most commonly reported eutrophication-related problems included hypoxia, losses of submerged grasses, excessive algal blooms (the most commonly reported problem), and numerousoccurrences of nuisance and toxic HABs. The midAtlantic was identified as the region most impacted by eutrophication. The majority (almost 60%) of estuaries assessed, with the exception of North Atlantic systems (Cape Cod north to Maine), were highly influenced by human-related activities that contributed to land-based sources of nutrient loads.The most commonly reported causes of nutrientrelated impairments were agricultural activities (row crops and livestock operations), wastewater treatment plants, urban runoff, and atmospheric deposition. Eutrophication-related problems were predicted to worsen in 65% of estuaries, whereas 19% of the assessed estuaries were expected to improve in the future. Analysis of the extent of change in 58 estuaries from the early 1990s to the early 2000s shows that most (55%) were unchanged, including all of the larger estuaries. The principal sources of nutrients that sustain coastal eutrophication vary among systems. Where significant rivers enter the coastal zone, nutrient loads associated with the freshwater discharge are usually the predominant source. Coastal systems lacking substantial riverine inputs may receive a substantial fraction of nutrient inputs via submarine groundwater discharge or direct atmospheric deposition, as in Barnegat Bay, New Jersey (Kennish et al. 2007). The major anthropogenic sources of nutrients to coastal waters are row crop agriculture and animal operations, industrial and municipal wastewater discharges, nonpoint source runoff from urban and suburban areas, and atmospheric deposition (Figure 3). Human activities have been linked to a dramatic increase in nitrogen cycling in the natural environment. Watersheds can become “nitrogen-saturated”, for example, when further additions of nitrogen do not elicit a response from the ecosystem. In nitrogen-saturated watersheds, the surplus nitrogen will theoretically leak to streams, groundwater, or the atmosphere (Vitousek et al. 1997). Loss or degradation of riverine and coastal wetlands can also contribute to eutrophication of coastal waters because wetlands naturally trap and retain nutrients (e.g., Valiela and Cole 2002). In most cases, eutrophication has been caused by an anthropogenic increase in nutrient inputs. In a few cases, large but, nonetheless, natural sources of nutrients have been implicated in sustaining high primary productivity in coastal systems. For example, some watersheds in southwest Florida have naturally high concentrations of phosphates that can support high levels of primary production (Turner et al. 2006a). Natural upwelling of nutrient-rich deep ocean water into shallow areas also can support large blooms of phytoplankton (Glenn et al. 1996, Chan et al. 2008) and can result in hypoxia. Upwelling of nutrient-rich waters has been implicated in the development of severe widespread hypoxia and, for the first time (in 2006), anoxia on Oregon’s inner continental shelf (Grantham et al. 2004, Chan et al. 2008). Transport of hypoxic water from the continental shelf into coastal embayments has also been documented along the Oregon coast (Brown

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 18 

## Chapter 2

et al. 2007). These developments seem to be linked to impacts of climate variation on ocean processes, such as intensity of upwelling winds, oxygen concentration in upwelled water, and water column respiration (Grantham et al. 2004, Chan et al. 2008). More subtle upwelling has been observed along the New Jersey coast and has been implicated in development of nearshore hypoxia (Glenn et al. 1996, 2004). Both of these cases, however, contrast with the situation for most coastal areas undergoing eutrophication, where the source of increased nutrient input is land-based and attributable to anthropogenic causes. Hypoxia can be related in some cases to documented long-term increases in algal biomass. For example, the long-term increase in chlorophyll- a (a proxy for phytoplankton biomass) in the Chesapeake Bay, as documented by Harding and Perry (1997), was concurrent with the increase in hypoxia documented by Hagy et al. (2004). In a few cases, hypoxic events have been related to high biomass HABs. For example, the one-time hypoxic event in the New York Bight (Garlo et al. 1979, Boesch and Rabalais 1991) and hypoxia in the Peace River-Charlotte Harbor system (Turner et al. 2006a) were both related to high biomass algalblooms. Further, massive mortalities of bottomdwelling organisms were associated with the cascading effects of algal toxicity, hypoxia, anoxia, and hydrogen sulfide poisoning during a toxic HAB event in Florida in 2005 (Landsberg et al. 2009). 

# 2.4. Consequences of Hypoxia
2.4.1. Ecological Consequences Ecological effects of hypoxia in coastal systems can vary in both degree and scale (Figure 5). The specific concentration of dissolved oxygen below which various animals suffocate varies, but for estuarine and marine species, effects generally appear when oxygen drops below about 3 mg/L (Diaz and Rosenberg 1995, Ritter and Montagna 1999, Rabalais et al. 2001, Breitburg et al. 2001, Karlson et al. 2002). However, the behavior of some organisms (e.g., fish, larvae) can be negatively affected at oxygen concentrations as high as 4-4.5 mg/L (Whitmore et al

. 1960, Kramer 1987, Breitburg 1994). Toxic hydrogen sulfide is often present in waters nearly or completely devoid of oxygen and significantly reduces survival (Gamenick et al. 1996). Fish kills are an obvious and very unpleasant consequence of hypoxia. The frequency andmagnitude of fish kills have increased as nutrientrelated eutrophication has worsened both hypoxia and HABs (Thurston 2002, Thronson and Quigg 2008). Fish kills related to hypoxia have been noted in waterbodies on all U.S. coasts, but the incidence of fish kills does not capture the extent of hypoxia impacts. Fish kills are much less widespread than hypoxia itself and sometimes occur only when several factors are present along with hypoxia (e.g., HABs as in Corsica River, Maryland, Bricker et al. 2007) or when hypoxia develops in a way that prevents fish from escaping. Other effects of hypoxia are less obvious, but more pervasive and likely more important overall. These include shifts in spatial distribution of organisms, changes in community structure caused by emigration of fish and mobile invertebrates,

Figure 5. The range of ecological impacts exhibited as dissolved oxygen levels drop from saturation to anoxia (based on Diaz and Rosenberg 1995, Rabalais et al. 2001).

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 19 

## The Problem of Hypoxia in U.S. Waters

mortality of sessile (immobile) bottom-dwelling organisms, and alteration or blockage of normal migration routes of fish and invertebrates. Hypoxic habitats that are avoided by organisms are functionally lost from the system (Breitburg 2002, Rabalais and Turner 2001, Sagasti et al. 2001). Because oxygen thresholds for avoidance are usually higher than for survival (Figure 5), habitat loss due to hypoxia is far greater than would be suggested by calculations based on recruitment or survival tolerances. Changes in community structure and habitat loss associated with hypoxia can also propagate to other components of the food web. For example, impacts to animals that act as crucial food web linkages between algal producers and top predators (e.g., benthic suspension feeders) result in a decreased flow of energy to predators and an increase to microbes (Figure 6, Diaz and Rosenberg 2008). Avoidance of hypoxia can also reduce energy flow to predators. Hypoxia can cause reduced growth (Taylor and Miller 2001), lower reproduction (Marcus et al. 2004), and other physiological effects (Wu et al. 2003). Shrimp and crabs exposed to hypoxia, for example, become immunocompromised and may suffer increased susceptibility to disease and mortality from bacterial infections (Le Moullac et al. 1998, Holman et al. 2004, Burgents et al. 2005; Tanner et al. 2006). Hypoxia has contributed to the collapse or impairment of a number of commercially important fisheries worldwide, including Norway lobster in the Kattegat Sea (Baden et al. 1990) and bottom-dwelling fish in the Baltic and Black Seas (Breitburg et al. 2001, Mee 2006). Effectively separating impacts of hypoxia on exploited fishery populations from those due to overfishing can be challenging (Breitburg et al. 2009). Most likely, the effect of hypoxia is to decrease productivity and resilience of exploited populations, making them more vulnerable to collapse in the face of heavy fishing pressure. The ecological impacts of hypoxia may be understood in terms of the ecosystem services normally provided by a healthy ecosystem, but lost as a result of hypoxia (Table 2). A full assessment of ecosystem services lost helps bridge the gap between ecological functions lost and their impact on people. In some cases, though not without challenges, ecosystem services can be assigned a reasonable dollar value. In these cases, analysis of services helps convey the economic costs associated with ecological impacts. Fisheries yield is one ecosystem service that can be impacted both directly and indirectly by hypoxia. Mortality of fisheries species is a direct mechanism by which services are lost. Loss of forage for bottom-feeding fish and shellfish due to hypoxia is probably more important in most cases and also amounts to a loss of ecosystem services. In the Chesapeake Bay, seasonal hypoxia lasts about three months and reduces the Bay’s total benthic secondary production by about 5% (Diaz and Schaffner 1990), or roughly 75,000 metric tons of biomass (Diaz and Rosenberg 2008). This is enough to feed about half the annual blue crab catch for a year. In the northern Gulf of Mexico, severe seasonal hypoxia can last up to six months and reduces benthic biomass by about 212,000 metric tons when the hypoxic zone is 20,000 km 2 

### (Rabalais et al. 2001). This lost biomass could Figure 6. Conceptual view of how hypoxia alters ecosystem energy flow with example systems (modified from Diaz and Rosenberg 2008).

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 20 

## Chapter 2

feed about 75% of the brown shrimp catch for a season (Diaz and Rosenberg 2008). It is unknown whether these systems can recover secondary production lost to hypoxia during periods with normal oxygen levels. The Chesapeake Bay has a recovery period of approximately nine months, whereas the northern Gulf of Mexico requires six months for recovery of the lost benthic production. Well-oxygenated conditions outside of summer, however, may be inadequate to support recovery since many other growth requirements (especially appropriate water temperatures) are optimal in the

summer. One of the less obvious ecosystem services lost during hypoxia is sediment mixing by benthic organisms, or ‘bioturbation.’ Reworking of sediments via bioturbation promotes oxygenation of sediments, improving habitat for benthic animals and promoting biogeochemical feedback processes that reduce nutrient recycling and limit eutrophication. In particular, bioturbation promotes coupled nitrification and denitrification, which eliminates excess bioavailable nitrogen from the ecosystem. Loss of these services amounts to additional nitrogen loading, which must be offset by additional controls on inputs (Hagy et al. 2004). When bioturbation is eliminated due to hypoxia, nitrogen is returned to the water as bioavailable ammonium, often with phosphorus as well, reinforcing a cycle of eutrophication (Aller 1994, Kemp et al. 2005). Determining the ecological consequences of hypoxia on ecosystem services (Table 2) is often complicated by cumulative and interacting impacts involving a variety of stressors. Quantification of the relationship between hypoxia and impacts on Impacts on Ecosystem Services Example Reference
Loss of biomass

Direct mortality of fisheries species Long Bay Koepfler et al. 2007 Direct mortality of prey species Northern Gulf of Mexico Rabalais et al. 2001 Reduced growth and production Chesapeake Bay Diaz and Schaffner 1990
Reduced recruitment Patuxent River Breitburg 1992
Loss of biodiversity

Elimination of sensitive species Pensacola Bay Hagy et al. 2006 Reduced diversity Pamlico River Tenore 1972 Increased susceptibility to disease and other stressors St. Johns River Mason 1998 Lower food web complexity Neuse River Estuary Baird et al. 2004
Loss of habitat

Crowding of organisms into suboptimal habitats Neuse River Estuary Eby et al. 2005 Increased predation risks (both natural and fishing) Northern Gulf of Mexico Craig and Crowder 2005 Forced migration from preferred habitat St. Josephs Bay Leonard and McClintock 1999 Altered or blocked migration routes New York Bight Sindermann and Swanson 1980 Altered energy and biogeochemical cycling

Increased energy flow through microbes Corpus Christi Bay Montagna and Ritter 2006 Production of toxic hydrogen sulfide Dead end canals Luther et al. 2004 Release of phosphorus from sediments Lake Erie Hawley et al. 2006 Release of ammonia and ammonium from sediments Chesapeake Bay Cowan and Boynton 1996 Loss of denitrification Chesapeake Bay Lewis et al. 2007 

### Table 2. Principal ecosystem characteristics and services impacted by hypoxia. Ecosystem services are processes by which the environment produces resources that are important to humans. Lost ecosystem characteristics highlighted here are critical for supporting ecosystem services. Not listed here are impacts to aesthetics or services lost due to eutrophication in general.

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 21 

## The Problem of Hypoxia in U.S. Waters

commercial and recreational living resources are needed by managers in order to improve coastal management and policy decision-making. Recent research advances in this area are discussed in Section 3.2.
2.4.2. Economic Consequences There is a growing collection of literature on the ecological consequences of hypoxia, but economic evaluations are lacking. Economic effects attributable to hypoxia are subtle and difficult to quantify even when mass mortality events occur. Much of the problem is related to multiple stressors (habitat degradation, overfishing, HABs, and pollution) acting on targeted commercial populations as well as factors that impact fishers’ economics (aquaculture, imports, economic costs of fishing, and fisheries regulations). Economic impacts that stem from the effects of hypoxia on fishery stocks are mostly subtle and tied to ecological impacts through reduced growth and reproduction. Other economic costs imposed on fishers are related to increased time on fishing grounds and costs of searching for stocks (e.g., to reach more distant fishing grounds beyond areas impacted by hypoxia). How these costs translate to impact on profits is complex, however, because in addition to the ramifications of reduced quantity, the unit value of landings on the market affects its total value and must be considered when evaluating the economic impacts. Although quantifying costs of hypoxia-related mortality events is difficult, there are some published examples (Table 3). Hypoxia in the early 1970s in Mobile Bay, Alabama was estimated to have killed over $500,000 worth of oysters (May 1973). An even greater economic cost was associated with the declining stock size associated with mortality and poor recruitment of oysters in years with severe hypoxia. A modeling study in the Patuxent River in Maryland estimated that the net value of striped bass fishing alone would decrease over the long-term by over $145 million if the entire Chesapeake Bay were impacted by hypoxia, which would preclude fishing in other sites (Lipton and Hicks 2003). Impacts of hypoxia on the overall health of the striped bass population and impacts to other Chesapeake fisheries were not included in this estimate but would substantially Event Impacts considered in Estimate Estimated Economic Impact Economic Impact in 2009 dollars Hypoxia in Mobile Bay in 1970’s (May 1973) Mortality to oysters $500,000 $2,400,000

Modeled hypoxia in Patuxent River, Maryland (Lipton and Hicks 2003) Striped bass fishing and associated activities in Patuxent River* $100,000** for dissolved oxygen below 5 mg/L in the River
$300,000** for anoxia in the River
$145,000,000** for dissolved oxygen less than 3mg/L in entire Chesapeake Bay (eliminates substitute fishing sites) $110,000**

$340,000**

$166,000,000**

New York Bight hypoxic event in the summer of 1976 (Figley et al. 1979) Surf clams, finfishes, ocean quahogs, sea scallops, lobsters $70,000,000 $265,000,000

* Note that the impact would be substantially greater if all target species were considered. **Net present value; the net present value represents the chronic ef fects of hypoxia on the value of striped bass fishing. 

### Table 3. Examples of hypoxia-related economic impacts. For a current perspective, impacts are also shown in 2009 dollars, calculated from the original values using the consumer price inflation index (http://www.bls.gov/cpi/).

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 22 

## Chapter 2

Climate Driver Direct Effect Secondary Effect Influence on Hypoxia
Increased temperature More evaporation Decreased stream flow

- Land-use and cover changes +/-
Less snow cover More nitrogen retention

- Warmer water Stronger stratification + Higher metabolic rates + More precipitation More stream flow Stronger stratification + More nutrient loading + More extreme rainfall Greater erosion of soil phosphorus + Less precipitation Less stream flow Weaker stratification

- Less nutrient loading

- Higher sea level Greater depth Stronger stratification + Greater bottom water volume

- Less hydraulic mixing + Less tidal marsh Diminished nutrient trapping + Summer winds and storms Weaker, less water column mixing More persistent stratification +

Stronger, more water column mixing Less persistent stratification

-

Shifting wind patterns Weaker/stronger upwelling potential +/- 

### Table 4. Estimated influence of climate drivers on the extent and severity of hypoxia (+ = more hypoxia) (Diaz 2008, Modified from Boesch et al. 2007).



### increase the overall economic consequences to fishers in the region. During the 1976 summer hypoxic event in the New York Bight, acute economic losses were estimated to have been over $70 million (Figley et al. 1979). Much of this loss was due to impacts to the surf clam resource, accounting for more than $60 million. These values consider short-term impacts from actual losses reported by fishers and potential losses due to resource mortality. Experience with hypoxic zones around the globe shows that both ecological and fisheries impacts become progressively more severe as hypoxia worsens (Diaz and Rosenberg 1995, Caddy 1993) and the area of suitable habitat declines (Bricker et al. 2006). Large systems around the globe have suffered serious ecological and economic consequences from seasonal hypoxia; most notably the Kattegat and the Black Sea. Consequences range from localized loss of catch and recruitment failure to complete system-wide loss of fishery species (Karlsen et al. 2002, Mee 1992). In systems where habitat is only partially lost to hypoxia, such as Long Island Sound and the Chesapeake Bay, catch rates for recreational fishing decline (Bricker et al. 2006). 

# 2.5. Future Considerations
2.5.1. Climate change Climate change will almost certainly influenceboth naturally occurring and eutrophicationrelated hypoxia, as well as the incidence of other ecological problems, such as HABs. To fully understand environmental change, it is necessary to consider the influence of multiple climate drivers (Table 4). In general, the expected long-term ecological changes favor progressively earlier onset of hypoxia each year and, possibly, longer overall duration (Boesch et al. 2007). Increasing average water temperature is one mechanism by which climate change may increase susceptibility of systems to hypoxia. Higher water temperatures promote increased water column

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 23 

## The Problem of Hypoxia in U.S. Waters

stratification, decreased solubility of oxygen, and increased metabolic rates, including oxygen consumption and nutrient recycling. Climate predictions also suggest large changes in precipitation patterns, but with significant uncertainty regarding what changes will occur in any given watershed (Christensen et al. 2007). Increased precipitation can be expected to promote increased runoff of nutrients to estuarine and coastal ecosystems and increased water column stratification within these systems, contributing to more severe oxygen depletion (Table 4, Global Climate Change Impacts in the U.S. 2009, Justic ́ et al. 2007, Najjar et al. 2000). Climate predictions for the Mississippi River basin suggest a 20% increase in river discharge (Miller and Russell 1992), which is expected to increase the average extent of hypoxia on the northern Gulf of Mexico shelf (Greene et al. 2009). Climate-associated changes in oceanic wind patterns can impact ocean circulation at a large scale. The severe hypoxia that has developed every year since 2002 along the coast of Oregon has been linked to climate-based changes in regional wind patterns, which affect water column stratification and delivery of nutrients from deep water to relatively shallow coastal areas.
2.5.2. Impacts of Biofuels Strategy The EISA of 2007 mandates production of 36 billion gallons of biofuels by the year 2022, with a 15 billion gallon limit for the amount to come from corn. Compared to other potential sources of biomass for biofuel production, such as perennial grasses or soybeans, corn is less efficient at taking up applied nutrients and usually requires higher nutrient application rates—these factors contribute to higher nutrient loadings (especially nitrogen) with corn (CBC 2007, NRC 2007). Increased production of corn-based ethanol biofuel is projected to exacerbate hypoxia in the Gulf of Mexico and other coastal areas (Greene et al. 2009, Rabalais et al. 2009). Donner and Kucharik (2008) estimate that expanded corn cultivation will increase the average annual flux of dissolved inorganic nitrogen to the Gulf of Mexico by 10- 34%. The acreage planted in corn grew by 19% between 2006 and 2007, replacing acreage in conservation reserve programs as well as soybeansand cotton (crops that are frequently less nitrogenintensive, particularly in the Upper Midwest; see Potter et al. 2006) (Turner et al. 2008, NASS 2007). However, this increase did not continue as high fertilizer prices, favorable prices for other crops, and a return to normal crop rotations led to a 7% decline in corn production in 2008 (NASS 2008). The EISA mandates that the U.S. biofuels strategy be ‘sustainable.’
2.5.3. Impacts of Future Management Decisions The extent of hypoxia in the future will depend on land management practices including agricultural practices.. Climate change will affect water column stratification, organic matter production, nutrient discharges, and rates of oxygen consumption. Land management will Figure 7. Relative magnitude and contribution (the larger the arrow, the larger the contribution) of land management practices versus climate change factors to expansion or contraction of low dissolved oxygen (modified from Diaz and Breitburg 2009).

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 24 

## Chapter 2

affect nutrient budgets and concentrations of nutrients applied to land through agriculture (Figure 7). For example, the expansion of agriculture for production of crops to be used for food and biofuels will, lacking preventive actions, result in increased nutrient loading to coastal waters and increased eutrophication effects (U.S. EPA 2007, Rabalais et al. 2007). On the other hand, improved agricultural conservation practices and large-scale implementation of nutrient best management practices (BMPs) can create dramatically better outcomes for water quality. The development of cellulosic ethanol production will enable farmers and forest managers to derive income from their lands while reducing nutrient runoff. This is an important aspect of the plans to achieve water quality improvements in the Chesapeake Bay region (Chesapeake Bay Commission and Commonwealth of Pennsylvania 2008). Application of these alternatives on agricultural lands that contribute disproportionately high nitrogen loads (e.g., tile-drained fields) will be especially beneficial. More widespread and aggressive implementation of nutrient removal technologies in wastewater and stormwater management also offers opportunities to reduce nutrient enrichment, eutrophication, and hypoxia. Together, comprehensive nutrient management has the potential to offset the impact of human population pressure, which otherwise will likely continue to be the main driving factor in the persistence and spreading of coastal hypoxia.

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 25 

# Federal Hypoxia and **Watershed Science Research: Status and Accomplishments**  Chapter 3



# T he serious and complex environmental issue of hypoxia and eutrophication cannot be studied or managed by any one Federal or state agency, but requires an organized, comprehensive approach. This chapter highlights Federal research accomplishments since 2003 when the last HABHRCA-mandated hypoxia assessment was compiled (CENR 2003). The Federal research presented herein is integral for informed decision-making and successful management of nutrients and hypoxia in an “adaptive management framework” (Figure 8). Under this framework, current scientific knowledge guides development of management goals with the option to continue to assess knowledge gaps. As scientific understanding advances, management goals are reassessed and adjusted as needed. Approaches taken in the Gulf of Mexico (Box 4), Chesapeake Bay (Box 5), and Long Island Sound (Box 6) provide good examples of the use of science to inform management decisions. In addition, regional governance structures involving multiple state and Federal partners are emerging as an important mechanism for mitigating hypoxia because most cases of coastal hypoxia are linked to nutrient loads resulting from watersheds that cross state boundaries. Examples include the Gulf of Mexico/ Mississippi River Watershed Nutrient Task Force, Gulf of Mexico Alliance, Gulf of Mexico Program, Great Lakes Regional Collaboration,

Figure 8. Conceptual diagram explaining how, in an adaptive management framework, scientific research informs management of environmental problems such as hypoxia (and vice versa).

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 26 

## Chapter 3

Chesapeake Bay Program, and Puget Sound Partnership. Many of the case studies presented in Appendix II provide additional descriptions of regional governance structures. Given the exponential increase in the number of systems experiencing hypoxia (Diaz and Rosenberg 2008), it is critical that more states take actions to reduce nutrient transport into the coastal zone. As highlighted in this chapter, each of the various research efforts conducted by Federal agencies or funded extramurally by Federal agencies has focused on understanding a different aspect of the hypoxia issue (Figure 9). Studies have addressed approaches for reducing nutrients at their sources, what happens to nutrients during transit through the watershed, how nutrients affect hypoxia and related water quality concerns in the coastal zone, and, finally, how hypoxia impacts aquatic life and ecological services provided by coastal systems. Recipients of extramural funds have included state governments and academic organizations that have coordinated their research with Federal research programs (see Appendix I). Many Federal agencies contribute to research and management of hypoxia and nutrients. The primary agencies include NOAA, EPA, USGS, and USDA

. NOAA has focused research funds and internal capabilities on monitoring and improving understanding of hypoxia and its impacts on commercially- and ecologically-important living resources in coastal waters. EPA bridges the continuum from freshwater ecosystems to estuaries and coastal waters and has focused resources on understanding and regulating nutrient inputs, such as those from wastewater treatment plants, as well as studying and modeling hypoxia in coastal
waters. USGS provides critical data through the measurement and modeling of freshwater and nutrient delivery to coastal waters throughout the Nation. Finally, USDA is responsible for developing and implementing strategies to reduce nutrient inputs to coastal waters from agricultural lands and urban ecosystems, which is a major cause of eutrophication and hypoxia in many systems. Recent research accomplishments for these Federal agencies are highlighted in this chapter and in the place-based case studies in Appendix II. Appendix I provides details about programs in Federal 

## Box 4. Adaptive Management Approach for Gulf of Mexico Hypoxic Zone

HABHRCA 1998 mandated an integrated scientific assessment of hypoxia in the northern Gulf of Mexico. The findings on how and why hypoxia forms in the Gulf informed the first Action Plan signed by Federal and state agencies in 2001. The final recommendation of the 2001 Action Plan called for an updated scientific assessment to be completed in five years which in turn would lead to an updated Action Plan. Following the release of the 2001 Action Plan, the Task Force completed a series of reports, including A Science Strategy to Support Management Decisions in 2004 and The Management Action Review in 2006. A series of four state-of-science symposia were also completed in 2006. These symposia and reports fed into a comprehensive EPA Science Advisory Board Report. The findings from the reassessment directly informed the 2008 Action Plan, which was signed in June 2008. The 2008 Action Plan recommends at least a 45% reduction in nitrogen and phosphorus flowing into the Gulf to reduce the size of the hypoxic zone to the 5000 km 2 target. Unfortunately, despite the positive intentions embodied in these various rounds of assessments and plans, the aerial extent of hypoxia in the Gulf has continued to grow, which indicates that an even broader, more forceful, perhaps regulatory, approach is needed.

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 27 

## Federal Hypoxia and Watershed Science Research

agencies which address various aspects of hypoxia. Where appropriate, the Federal agency responsible for conducting or funding specific research accomplishments is parenthetically highlighted in blue text. 

# 3.1. Improving Understanding of Hypoxia in Coastal Waters
Identifying and characterizing the causes of hypoxia are critical for effective ecosystem management and depend heavily upon monitoring programs and the development of models. Differences in factors that cause hypoxia among systems, ranging from primarily nonpoint source nutrients in the northern Gulf of Mexico to wastewater treatment plant effluent in Narragansett Bay (Figure 3), highlight one source of complexity in mitigating and controlling hypoxia in different coastal systems. The following sections highlight recent monitoring (Section 3.1.1) and modeling (Section 3.1.2) activities and advances in understanding of hypoxia characteristics and causes (Section 3.1.3).
3.1.1. Monitoring Hypoxia Monitoring of dissolved oxygen in coastal waters is usually conducted as a component of research and water quality monitoring programs. Many such programs are conducted through partnerships involving one or more agencies of the Federal government, one or more states, and local government. EPA ’s National Estuary Program (NEP) supports such partnerships around the country in order to implement environmental monitoring. As part of their respective missions, EPA and USGS conduct long-term assessments of 

## Box 5. Adaptive Management Approach for Chesapeake Bay

In 1983, following the conclusion of a 5-year EPA Chesapeake Bay Study and other water quality initiatives, Bay states and the Federal government agreed to reduce nitrogen inputs into the Bay through the first Chesapeake Bay Agreement. A Scientific and Technical Advisory Committee was formed at this time and released its first report in 1986. The scientific information and consensus in this report led to the Second Chesapeake Bay Agreement in 1987, calling for a basin-wide nutrient reduction strategy and more stringent nutrient reduction targets. Reevaluations in 1992 and 1997 then led to the Chesapeake 2000 Agreement

, which recommitted to goals of the prior agreements while outlining more specific strategies for achieving the targets. In support of the adaptive management process, the Chesapeake Bay Program recently agreed to a framework for a five-stage adaptive management model (Kaplan and Norton 2008). A recent assessment of water quality trends in rivers feeding the Bay showed significant improvements for nutrient loading for nitrogen (72%), total phosphorus (81%), and sediment (43%), indicating that management actions are having some effect in reducing nutrients and sediments (Langland et al. 2006). However, to date, the management of nutrient input in the Chesapeake Bay has not improved dissolved oxygen, although it has caused small-scale reversals in hypoxia (Diaz and Rosenburg 2008). Figure 9. Schematic describing general areas of hypoxia-related research. This illustration explains the organization of Chapter 3.

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 28 

## Chapter 3

environmental and ecological conditions, including dissolved oxygen, within selected bays, estuaries, and other coastal waters. The Integrated Ocean Observing System (IOOS; NOAA ), which seeks to integrate all ocean and coastal data within a national monitoring framework, does not currently include dissolved oxygen as one of its five high priority variables for synthesis. However, some of the regional ocean observing systems that contribute to IOOS collect dissolved oxygen data (Appendix I). There is regionally focused monitoring of dissolved oxygen in the Chesapeake Bay ( EPA
, NOAA, USGS ), Narragansett Bay ( NOAA, EPA ), Hood Canal, Lake Erie ( NOAA ), Long Island ( EPA ), and along the Oregon continental shelf ( NOAA, National Science Foundation or NSF ; see Figure 10 for examples). Most of the 27 National Estuarine Research Reserves ( NOAA –state partnerships, recently summarized by Sanger et al. 2002) monitor dissolved oxygen through the System-Wide Monitoring Program (SWMP). Finally, routine monitoring of dissolved oxygen as part of juvenile salmonid surveys from Oregon to the Canadian border along the shelf began in 2006 in order to monitor the upwelling dynamics that have caused periodic hypoxia off the coast of Oregon ( NOAA ). Monitoring in the northern Gulf of Mexico, the largest hypoxic zone in the United States (Figure 10d) and one of two hypoxic zones located in Federal waters (the other one is off the Oregon coast, see Appendix II) has received increasing attention in recent years. Since 1985, an annual shelf-wide survey has been conducted during July, when hypoxia is presumed to be most widespread. Although this has been funded as part of a competitive research program, the surveys have provided the principal metric for assessing progress toward the goal of a reduction in hypoxia outlined by the Gulf of Mexico/Mississippi River Watershed Nutrient Task Force (see Gulf of Mexico case study, Appendix II) and for validating hypoxia forecasts ( NOAA ). Other ship-based sampling of water quality (including dissolved oxygen) on the continental shelf and near major outlets of the Mississippi and Atchafalaya Rivers occurs annually and seasonally as a part of shelf-wide surveys and hypoxia-related research ( NOAA, EPA ). These surveys were designed to quantify key physical and biogeochemical processes influencing the development and persistence of hypoxia, and to support development of predictive models ( NOAA, EPA ). Seasonal field surveys using EPA ’s offshore survey vessel and satellite ocean color remote sensing were conducted in 2003, 2006, and 2007 to characterize the magnitude and variability in physical, biological, and chemical oceanographic conditions and processes along the northern Gulf continental shelf from the Mississippi River Delta to Texas ( EPA ). NOAA’s Gulf of Mexico Hypoxia Watch (http://ecowatch.ncddc.noaa.gov/hypoxia) has provided internet access to summer seasonal dissolved oxygen data collected through routine the Southeast Area Monitoring and Assessment Program (SEAMAP) groundfish monitoring cruises in June and July ( NOAA ). Several moored instrument arrays have also been deployed in the Gulf of Mexico hypoxic zone to obtain continuous records of dissolved oxygen. Efforts to improve and test the moored instruments are continuing ( NOAA, Minerals Management Service ).



## Box 6. Sound Science Leads to Significant Reductions in Hypoxia in Long Island Sound

Following progressive declines in water quality and increasing prevalence of hypoxia, the Long Island Sound Study (LISS) was formed in 1985 to guide the recovery of the Sound. The LISS released a Comprehensive Conservation Management Plan in 1994 that outlined 3-phases: 1) in 1990, freezing nitrogen loadings in critical areas to prevent worsening of hypoxia, 2) implementation of low-cost nitrogen reduction measures, and 3) reduction of nitrogen loads to target levels. In 1998, the LISS adopted a 58% nitrogen reduction target by 2014 and in 2001, the EPA approved state total maximum daily loads (TMDL) plans to achieve this goal. By 2005, point source nitrogen loads had been reduced by 20% from the peak in the early 1990’s and the severity of hypoxia declined (see Long Island Case Study in Appendix II).

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 29 

## Federal Hypoxia and Watershed Science Research

Recognizing the need to expand coastal monitoring in the northern Gulf of Mexico and move funding from a competitive research mechanism to an operational monitoring framework, NOAA convened a workshop in January 2007 entitled “Summit on Long-Term Monitoring of the Gulf of Mexico Hypoxic Zone: Developing the Implementation Plan for an Operational Observation System.” Three committees were formed as a result of the Summit (executive, technical, and stakeholder) and have worked to develop a monitoring implementation plan, which includes detailed designs and cost estimates. This plan was completed in December 2008. Operational monitoring for the northern Gulf of Mexico is important for tracking progress in remediating hypoxia and support forecasts and other scientific research needed to inform management. Implementation of this plan is a priority. 

## Box 7. Hypoxia Advanced Warning Protects Drinking Water

Since 2006, when three out of four Cleveland water treatment plans were exposed to anoxic waters carrying increased loads of certain metals, NOAA deployed water quality monitoring sensors as part of its Real-time Coastal Observation Network (ReCON) near the plants’ water intakes. The ReCON sensors provided advanced warning of hypoxia in 2007, allowing the Cleveland Water Division to adjust their water treatment approach to maintain the safety of water supplies for 1.5 million people.

Real-time Coastal Observation Network (ReCON)

Figure 10. Hypoxia is most intensively monitored in the largest and most impacted coastal systems in the United States. Examples include: a) Long Island Sound, b) Chesapeake Bay, c) Lake Erie, and d) Northern Gulf of Mexico. 

### a) b)

c)

d) Image: EcoCheck and Chesapeake Bay Program

Image: IFYLE Program Image: CT DEP

Image: LUMCON, NOAA

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 30 

## Chapter 3

3.1.2. Modeling and Forecasting Hypoxia Environmental models can range from simplestatistical equations to complex, computerintensive three-dimensional simulations that incorporate various aspects of physical, chemical, and biological processes in a coastal waterbody and its watershed. Models are essential tools for making scientific inferences when systems are too large or complex to conduct manipulative experiments. Weather forecast models are the most widely appreciated environmental models, but environmental models are also widely used for air quality forecasting and management, fisheries management, emergency management, and water quality management, among others. Models are particularly useful for understanding hypoxiabecause hypoxia reflects strongly coupled physicalchemical-biological processes. Computer intensive water quality models have been used for nearly three decades to predict the impact of nutrient management actions on specific water quality outcomes, including the extent and severity of hypoxia. Coupled watershed-water quality models have been a critical tool in the effort to manage nutrients, reduce hypoxia, and, more broadly, restore the health of the Chesapeake Bay ecosystem. The EPA Chesapeake Bay Program has funded water quality model development by the U.S. Army Corps of Engineers Waterways Experiment Station and others since 1987 and has relied heavily on the resulting models to guide nutrient reduction strategies throughout the Chesapeake Bay watershed (Koroncai et al. 2003; Cerco and Noel 2004, 2005). Model results provide options to be analyzed in the course of determining a nutrient management strategy. A deliberative process based on model results is necessary because all models, even if very useful, are imperfect and subject to error (Koroncai et al. 2003). While efforts to improve Chesapeake Bay modeling are ongoing, recent Federal modeling efforts have focused heavily on model development in the northern Gulf of Mexico hypoxic zone, which has followed a somewhat different trajectory. A variety of relatively simple models Table 5. Models developed since 2002 to forecast or simulate Gulf of Mexico hypoxia (adapted from Justic ́ et al. 2007).

Model Class Key Outputs Key Attributes
Greene et al. (2009) Statistical Substantial and sustained nitrogen and phosphorus reductions are required to reduce hypoxia. Indicated dependence of conclusions on model choice and input data and provides estimates of forecast uncertainty.
Justic ́ et al. (2002) Simulation Riverine nutrients play a major role in the development of hypoxia. Provides estimate of time series of oxygen at a single location. Includes mechanistic detail.
Scavia et al. (2003, 2004) Simulation Extensive hypoxia not common before mid-1970’s. A 40-45% reduction is nitrogen may be required to meet management goals. Provides estimates of forecast uncertainty using a biophysical modeling scheme.

Stow et al. (2005) Statistical Stratification is a predictor of hypoxia and the amount of stratification required to induce hypoxia has gone down (1982-2002). Uses statistical approach to characterize long-term change in hypoxia relative to stratification strength.

Turner et al. (2005, 2006) Statistical Hypoxic zone has only recently developed (1970’s or 1980’s) and nitrogen is the major driving factor controlling the size. Incorporates spring nitrate, river discharge, and year factor to predict hypoxia

Hetland and DiMarco (2008) Simulation Biological processes controlling hypoxia vary from east to west. Hypoxia is formed in place, and not advected across shelf. Uses 3-dimensional hydrodynamic model with simplified water quality model to explore implications of different controls on oxygen consumption for the spatial distribution of hypoxia.

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 31 

## Federal Hypoxia and Watershed Science Research

have been developed to forecast the response of hypoxia to various nutrient load scenarios. In addition, more complex models have been and are being developed to improve understanding of basic scientific questions (Table 5). Coupled hydrodynamic-water quality models have been developed for the northern Gulf but these models lack important mechanistic details, particularly in their representation of biogeochemical and other biological processes (Table 5). Despite being recently developed and still relatively basic, models have nonetheless been very influential in setting nutrient reduction targets for hypoxia in the northern Gulf of Mexico (U.S. EPA 2007, Justić et al. 2007; NOAA ). Simple regression models relating hypoxia to either nutrient loading (Turner et al. 2006b; NOAA ) or freshwater flow and nutrient concentration (Greene et al. 2009; EPA ) have proven surprisingly predictive. The model by Turner et al. (2006b) has been used annually since it was published to forecast the expected extent of hypoxia in midsummer. A suite of regression models (Greene et al. 2009; EPA ) explore how model selection may influence possible conclusions and applications. Both studies suggest that a decrease in nutrient loads from the Mississippi Atchafalaya River Basin (MARB) on the order of 45% will be sufficient to reduce the extent of hypoxia on the shelf to the goal (five-year average of 5,000 km 2 

### ) established in the first Gulf Hypoxia Action Plan. Turner et al. (2006b) suggest that hypoxia was not present on the shelf prior to the mid 1970s, whereas Greene et al. (2009) conclude that hypoxia likely occurred as early as the 1950s, but most likely was much less extensive and perhaps did not occur every year as it presently does. Scavia et al. (2003, 2004; NOAA ) employ a biophysical model to parameterize the physical regime on the northern Gulf of Mexico shelf and forecast hypoxia extent (Figure 11). Scavia et al. (2003, 2004) and Greene et al. (2009) each provide models that forecast the extent of hypoxia as a function of nutrient inputs with quantified bounds of forecast uncertainty, a useful development for management applications. These essentially empirical models provide a scientific rationale for the 45% reduction in both nitrogen and phosphorus loading that has been suggested to be needed in order to achieve the hypoxia reduction target for the Gulf. They lack, however, explicit simulation of ecological processes that might be used to better address fundamental questions regarding ecological controls on the extent and intensity of hypoxia as well as the impacts of hypoxia. For these applications, process-oriented dynamic simulation models would be preferable. These models wouldbe more similar to the coupled hydrodynamicwater quality models that have been developed for the Chesapeake Bay (Cerco and Noel 2004, 2005). For simulation models, the Regional Ocean Model System (ROMS), a multi-purpose, multidisciplinary, open source oceanic modeling tool, is being adapted to Narragansett Bay and will be coupled with an ecological model to improve understanding of the conditions driving hypoxia in that system ( NOAA ). The ROMS model is also now functional for the Chesapeake Bay, and

Figure 11. Ensemble forecasts of the response of hypoxia to changes in riverine nitrogen load . Loads are percent reduction from the 1980–1996 mean May–June total nitrogen loads. The grey shaded area, representing the ensemble forecast, contains all values from 1,000 simulations. The horizontal bar at 5,000 km 2 represents the Action Plan goal. The blue area represents the range of nitrogen load reductions needed to achieve that goal. Most of the Gulf of Mexico hypoxia models agree that an approximately 45% reduction in both nitrogen and phosphorus are required to reduce the size of the hypoxic zone to the goal set by the Mississippi River/Gulf of Mexico Watershed Nutrient Task Force (adapted from Scavia et al. 2003, 2004).Hypoxic Zone Area (km)

N Load Reduction

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 32 

## Chapter 3

a new project is focused on adding ecological and sediment components to predict impacts of climate and nutrients on hypoxia and the ecosystem ( NOAA ). The ROMS ecological hybrid model is also being adapted for the finer-scale resolution existing in the shallower, narrower Delaware Inland Bays which periodically experience fish kills due to severe diel hypoxic events ( NOAA ). A modified version of the ROMS model uses a number of oceanographic and meteorological variables to examine the effects of water column stratification and circulation on hypoxia in the northern Gulf of Mexico (Hetland and DiMarco 2008, NOAA ). Additionally, Justic ́ et al. (1996, 2002; NOAA ) developed another simulation model, a coupled biological-physical model to examine oxygen cycling dynamics in the core of the hypoxic zone. This model used a number of forcing functions including nitrate loads, monthly Mississippi River runoff, and oceanographic and meteorological conditions. It enabled the simulation of historical oxygen concentrations and the linking of Mississippi River nutrient loadings to eutrophication.

Models can also be used to assess estuarine system susceptibility to hypoxia as a result of nutrient enrichment. An initial version of a Nitrogen-Phytoplankton-Detritus-Oxygen (PDO) model is being developed to address estuarine susceptibility to nutrient enrichment, considering the effects of nutrient loading, flushing, stratification, denitrification, and temperature ( NOAA ). A preliminary PDO model is being tested on 87 estuaries and shows promise in predicting chlorophyll- a concentrations across a range of estuarine types. The model could be used to determine which estuaries, especially ones not currently impacted, are most susceptible to secondary effects of enrichment. This information could help drive preventative management actions.
Finally, as part of the International Field Years on Lake Erie (IFYLE, NOAA ), an international government and academic partnership, a major effort has been underway since 2005 to model various aspects of the Lake Erie ecosystem with a special emphasis on understanding the causes and dynamics of hypoxia. In an effort to develop a modeling framework that could help synthesize the data collected during IFYLE, a new project will employ a number of modeling approaches (e.g., watershed, river hydrology, lake hydrodynamics, lower food web, spatially explicit higher food web, statistical) to help identify the causes of hypoxia in Lake Erie and its consequences for the Lake Erie food web ( NOAA )

. 3.1.3. Identifying Causes and Characteristics of Hypoxia Chapter 2 provides an overview of the general causes of hypoxia. In addition, Appendix II covers a series of specific case studies which provide additional detail about causes and characteristics of hypoxia in highlighted systems. In general, advances have been made in understanding the relationship between various nutrients and the size or extent of hypoxia. For instance, determining that phosphorus is a limiting nutrient for primary production on portions of the continental shelf in northern Gulf of Mexico waters led to a call for reduction in both nitrogen and phosphorus levels being transported down the Mississippi River ( NOAA, EPA ). In addition, there has been improved understanding of the influences that physical oceanographic variables (e.g., salinity and currents) have on hypoxia formation and characteristics. Understanding what aspects of the physical system allow hypoxia to develop and tracking how it changes through time have been active areas of research for most systems that experience hypoxia. Additionally, hypoxic waters tend to remain near where they were formed, suggesting that currents do not move hypoxia across the shelf ( NOAA ). The structural features (horizontal and vertical characteristics) of hypoxia have been accurately reproduced using a combination of benthic respiration and hydrodynamic models. This has reaffirmed the observed spatial and temporal variability of the hypoxia, especially towards the end of the hypoxia season. Different processes regulate the formation of hypoxia in different regions of the hypoxic zone. Additionally, despite being disrupted by hurricanes (which serve to mix the water column and oxygenate the bottom

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 33 

## Federal Hypoxia and Watershed Science Research

layer), the hypoxic zone can be resilient, quickly reforming once the storm has passed ( NOAA ). The physical circulation of Narragansett Bay has been well studied through the use of strategically deployed shipboard and moored instruments. Recent results show that stratification is a major driver for hypoxia, with hypoxia happening most often in summer several days after a neap tide ( NOAA ). Meanwhile, efforts to determine the causes of hypoxia off the Oregon shelf (e.g., Chan et al. 2008) are being pursued after hypoxia and anoxia in waters less than 50 m deep caused massive mortality of benthic organisms ( NOAA, NSF ). Upwelling dynamics are important for many of the west coast systems experiencing hypoxia such as Yaquina Bay and Hood Canal (see Appendix II). National assessments by a variety of Federal agencies and Federal-state partnerships, with the support of academic scientists, have revealed and characterized widespread nutrient pollution in estuarine waters (CENR 2000, 2003; NRC 2000; U.S. Commission on Ocean Policy 2004). The EPA Water Quality Inventory reported that 17% of the estuarine waters assessed were impaired by low dissolved oxygen due to anthropogenic eutrophication (U.S. EPA 2002). EPA ’s NEP and National Coastal Assessment Program and the NOAA ’s National Centers for Coastal Ocean Science have been formally monitoring and assessing individual estuaries since the early 1990s and have broadly reported that estuarine water quality has been degraded by excess nutrients and eutrophication (U.S. EPA 2005, 2008; Bricker et al. 1999, 2007).

The first National Estuarine Eutrophication Assessment (Bricker et al. 1999, NOAA ), a survey of the extent, severity, types, probable causes, and future outlook of eutrophic symptoms, was conducted in the early 1990s. An update, examining the status of eutrophication in the early 2000s and the changes that have occurred since the early 1990s, was completed in 2007 (Bricker et al. 2007). For both reports, data and information were collected for 141 estuaries and coastal waterbodies from scientists and resource managers from academia, state, and Federal agencies (i.e., USGS, EPA
, and NOAA ), as well as nongovernmental organizations. This national assessment emphasizes the need for a coordinated and integrated effort that balances management action, efficient monitoring to assess the effectiveness of the management, focused research, and a communication campaign aimed at engaging the broader public. 

# 3.2. Quantifying and Modeling the Impacts of Hypoxia As discussed in Chapter 2, quantifying the impacts of hypoxia remains a critical information gap. The state-of-knowledge related to hypoxia impacts, as well as the research and management needs, were assessed in the NOAA -supported “Ecological Impacts of Hypoxia on Living Resources Workshop,” which was held in March 2007. A peer-reviewed special issue of the Journal of Experimental Marine Biology and Ecology
, which resulted from the 2007 workshop and contains the most up-to-date knowledge of hypoxia impacts, was published in 2009 (Lewitus et al. 2009). The major results from this workshop and Figure 12. Trends in catch per unit effort for brown shrimp in the northern Gulf of Mexico (modified from Downing et al. 1999).

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 34 

## Chapter 3

other Federally funded research are presented below. Quantifying the ecological impacts of hypoxia is essential for developing meaningful targets for hypoxia mitigation strategies (e.g., goals for areal extent or periodicity of hypoxia). A number of effects on living resources have been documented in both field and laboratory experiments. Many of these effects are dependent on both the temporal and spatial scale of hypoxic events and can be manifested through both direct and indirect impacts. Current research suggests that effects at both the population and individual levels are likely to be revealed not only through direct means, such as fish kills, but also through complex indirect, often sub-lethal mechanisms, such as reduced reproductive output and growth. However, separating the effects of hypoxia from other stressors that also negatively influence populations and individuals is extremely difficult. For example, trends in the catch per unit effort of brown shrimp in the northern Gulf of Mexico are consistent with impacts from hypoxia, habitat loss, overfishing, and economic factors (Figure 12). But the area of hypoxia may have a greater influence on annual landings (Figure 13). Models which relate experimental field and laboratory studies to population-level changes in fisheries and potential economic impacts are being developed for several systems including the Neuse/Pamlico River estuary, Lake Erie, Chesapeake Bay, Delaware Inland Bays, and northern Gulf of Mexico ( NOAA ).
3.2.1. Mortality and Altered Distribution of Fauna Faunal mortality (e.g., fish kills) has been documented in most coastal systems affected by hypoxia and is often a factor of the duration and extent of hypoxic conditions. Most of the species affected are invertebrates that are unable to move (e.g., clams) when dissolved oxygen becomes too low. This has been best documented in the Chesapeake Bay, where summer hypoxia typically causes mortality among immobile bottom-dwelling organisms (e.g., clams, mussels, and worms) in deep water (see Seitz et al. 2009). Similarly, mortality events in the northern Gulf of Mexico are typically limited to immobile benthos within the hypoxic zone, although mortality of mobile fauna has also been reported (see Rabalais et al. 2001). Elsewhere, the increasing frequency of transient hypoxic events in Puget Sound has resulted in large localized die-offs of fish and shellfish species, while die-offs of menhaden and other species have been increasing in frequency in the Neuse River, North Carolina and in Narragansett Bay, Rhode Island. These areas were also sites of benthic faunal die-offs, especially in Narragansett Bay where hypoxia has resulted in localized loss of blue mussel populations (Altieri and Witman 2006). Although it has been long known that mobile organisms, especially fish, avoid hypoxic waters, the extent and pattern of movements have only recently been documented. In most systems, including the Chesapeake Bay and the northern Gulf of Mexico, virtually all bottom-dwelling mobile species (e.g., Atlantic croaker, Craigand Crowder 2005), as well as some openwater species (e.g., bay anchovy, Taylor et al. 2007) are absent in bottom waters of hypoxic areas, while in Lake Erie fish are typically not found beneath the thermocline where dissolved oxygen can be low (Ludsin et al. 2009). Within Figure 13. Relationship between annual landings of brown shrimp in the northern Gulf of Mexico and the size of the hypoxic zone (O’Connor and Whitall 2007).

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## Federal Hypoxia and Watershed Science Research

Delaware’s coastal bays, juvenile weakfish avoid hypoxic conditions caused by diel oxygen cycling, resulting in the daily emigration of weakfish out of tidal tributaries and their preferred habitats (Tyler and Targett 2007). Movements of Atlantic croaker and shrimp in the Gulf of Mexico have produced a “halo effect” which describes the congregation of fish around the edge of the hypoxic zone (Craig and Crowder 2005, Craig et al. 2005). Changes in zooplankton distribution have also been observed in Lake Erie, the Gulf of Mexico, and the Chesapeake Bay, with changes in biomass size spectra detected in the latter two systems. There have also been anecdotal reports that commercial fishing has adapted to hypoxia-induced altered spatial distribution, possibly placing concentrated prey at risk of overfishing. As would be expected, an analysis of fishery landings in 22 ecosystems that experience seasonal hypoxia (> 40% of total bottom area affected) determined that pelagic species such asClupeoid fish were more abundant than bottomdwelling species such as crustaceans. Systems experiencing increased nutrient loadings, but not hypoxia, experienced the opposite effect on species composition, with more bottom-dwelling crustaceans than pelagic fish (Breitburg et al. 2008). Hypoxia-induced population migrations likely reflect an effective loss of preferred habitat for some species (Figure 14). The congregation of Atlantic croaker and brown shrimp along the edge of the hypoxic zone in the Gulf of Mexico (the halo-effect) suggests a loss of preferred habitat. Further, white shrimp spend a critical portion of their life cycle within the Gulf of Mexico and can be excluded from optimal spawning habitat. Hypoxia in the Chesapeake Bay has reduced and fragmented habitat for endangered sturgeon, likely hampering the species recovery (Niklitschek and Secor 2005). In addition to exclusion from preferred physical habitat, migrational patterns of species have been altered both in the Gulf of Mexico (e.g., brown and white shrimp) and along the coast of New Jersey (e.g., blue fish, Garlo et al. 1979). 3.2.2. Changes in Food Web Structure and Physiology An emerging, indirect impact of hypoxia is the alteration in predator-prey relationships and food web functions that often involve complex pathways through cascading effects. Shifts in diets can result in changes in the transfer of energy through the food chain. Hypoxia-induced shifts in diet have been documented in a number of systems and typically result in shifts from bottom-dwelling to pelagic food items (Baird and Ulanowicz 1989, Baird et al. 2004). Additionally, the availability of suitable prey can be limited for planktivorous fish (fish that eat plankton) during severe hypoxia, due to hypoxia-induced changes in plankton distribution and segregation from predators (Zhang et al. 2009). Conversely, it has been hypothesized that the altered spatial distribution of organisms can also increase predation by concentrating prey, such as along the edge of a hypoxic area. There can also be benefits to both prey and predators, but these are species dependent. For example, in Narragansett Bay, quahogs benefited from reduced predation pressure during moderate hypoxic conditions, whereas blue mussels suffered high mortality under similar conditions and thus received no benefit from reduced predation (Altieri 2008). Varying tolerance to hypoxia can also increase the abundance of certain predators. For example, hypoxia can lead to increased relative abundance of gelatinous zooplankton (jellyfish and comb jellies) which are more tolerant to low dissolved oxygen,

Figure 14. Map of Gulf of Mexico with darker shaded areas indicating denser fish populations in the 1960s. Red line outlines current general configuration and geographic location of the hypoxic zone. Source: Kevin Craig, FSU. 

### Pre-Hypoxia Fish Catch (1961-1965)



### 1997 Dead zone: oxygen ≤ 2 mg/L 1997 Hypoxic zone: oxygen < 2 mg/L

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which in turn leads to increased predation on fish larvae. The overall effects of hypoxia on food web structure have only recently begun to be quantified, but appear to be both species- and system-specific. Sustained, long-term hypoxia can ultimately lead to a total shift in food web structure, which has been observed in several systems (Rabalais et al. 2001, Baird et al. 2004).Researchers are increasingly examining sublethal physiological, reproductive, and bioenergetic impacts of hypoxia. A reported effect of hypoxia in commercially- and recreationally-relevant species is growth reduction. In the Gulf of Mexico, hypoxia has been linked to reductions in brown shrimp lipid content as well as size. In laboratory analyses, striped bass and menhaden growth were reduced, while field examinations have found reduced growth in both juvenile summer flounder and weakfish. Reproductive effects include alterations to reproductive organs, reduced production of offspring, and lowered rates of offspring survival. Atlantic croaker have also exhibited altered endocrine function and suppressed growth of ovaries and testes under similar conditions. Reduced egg production attributed to the effects of hypoxia has been found in Atlantic croaker, grass shrimp, and naked gobies in the Chesapeake Bay and Gulf of Mexico estuaries (Thomas et al. 2007). Further, egg survival can be reduced when eggs spawned within the water column sink into hypoxic water, as has been observed with bay anchovy and weakfish in the Chesapeake Bay (Breitburg 2002). Finally, a number of other sublethal or indirect physiological effects of hypoxia have also been documented including immune suppression in oysters (Anderson et al. 1998) and reduced consumption and respiration in fish and shellfish. 

# 3.3. Monitoring and Modeling Nutrient Flux in U.S. River Systems Monitoring streamflow and nutrient fluxes in river systems that feed coastal estuaries is essential to understanding and predicting coastal hypoxia. Furthermore, knowledge of the sources of nutrients ultimately delivered to the coastal zone and knowledge of processes that reduce nutrient loads during transit are required for the development of effective management strategies for reducing coastal hypoxia. It is not economically feasible to monitor all the sources and fluxes of nutrients in all rivers and streams in the United States. However, models using monitoring data from targeted watersheds, representative in terms of their climate, geography, land use, and management measures, can be extremely valuable tools. These models can identify the geographic areas with the highest nutrient contributions, the human activities that contribute the most nutrients, the ecosystems that reduce nutrient fluxes, and potentially the management measures that are most effective at mitigating nutrient fluxes.
3.3.1. Monitoring Nutrient Sources and Fluxes to Coastal Systems There are two major components of riverine monitoring in the United States. The first component is monitoring delivery of streamflow and nutrient fluxes to coastal environments. This monitoring requires sufficient temporal resolution to enable development of cause and effect linkages between the timing of nutrient fluxes and the development of hypoxia. The second component comprises monitoring of inland watersheds. This monitoring happens in watersheds of various scales and supports modeling applications that define the spatial distribution of the sources of nutrients and the human activities that affect those fluxes.

The USGS is the primary agency monitoring streamflow, nutrients, and other water quality constituents in rivers and streams throughout the United States. Other agencies rely on these data to determine effective conservation measures to protect downstream resources. Additional monitoring is conducted by state governments through water quality compliance programs under EPA oversight. Although limited by the objective and design, more and more of these data are being integrated into regional and national monitoring efforts. An important element of the design of programs monitoring nutrient delivery to coastal systems

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## Federal Hypoxia and Watershed Science Research

is the availability of continuous streamflow records and water quality measurements taken with sufficient frequency to enable accurate estimation of nutrients fluxes (mass per unit time) with adequate temporal resolution. The U.S. Commission on Ocean Policy (2004) cited a critical loss of USGS stream gauging capacity due to funding constraints, which has limited the ability to monitor water and other constituents entering our coasts. Most monitoring programs are designed to measure the concentration of nutrients at the time of sampling, and measurements are only taken a few times each year. However, flux estimates are essential to evaluating the causal relationship between nutrient delivery and hypoxia and to evaluate trends, including the performance of management actions.

The USGS National Stream Quality Accounting Network was redesigned in 2007 as a national coastal monitoring network with the primary goal of measuring delivery of streamflow and the associated fluxes of nutrients and selected other chemical constituents to coastal environments. The redesign maximizes existing resources and ongoing monitoring programs (such as the USGS National Water Quality Assessment Program and state monitoring programs). In 2008, five additional stations were added to this national coastal monitoring network as a result of the initial steps of implementation of the National Monitoring Network of the Ocean Action Plan, thereby increasing the number of large coastal rivers being monitored from 13 to 18. These 18 stations were selected because the rivers they monitor contribute the greatest percent of streamflow and nutrient fluxes to coastal environments. Seventeen stations are in the conterminous United States; the other is on the Yukon River in Alaska. Together, these stations measure more than 80% of the total discharge of streamflow, nitrogen, phosphorus, and suspended sediment to U.S. coastal waters ( USGS ). Samples are collected at most stations twelve times each year to enable accurate annual flux estimates for most constituents measured. Increased frequency of measurements is required to estimate seasonal or monthly fluxes with greater certainty and also to capture episodic events. This monitoring network does not address delivery of nutrients by smaller rivers to many local coastal ecosystems affected by hypoxia. Monitoring of inland watersheds is conducted by a diverse group of local programs. However, as

**0 100**  **200 300**  **400 500**  **600 700** **1979 1980** **1981 1982** **1983 1984** **1985 1986** **1987 1988** **1989 1990** **1991 1992** **1993 1994** **1995 1996** **1997 1998** **1999 2000** **2001 2002** **2003 2004** **2005 2006** **2007 Avg. 79-07** **2008**

**Year Dissolved Nitrite plus Nitrate Flux as N,** **in Thousands of Metric Tons June**  **May April** 

**Figure 2. Estimated April, May, and June dissolved nitrite plus nitrate flux as N to the Gulf of Mexico for 1979**  **through 2008. Maximum, minimum, and average fluxes are determined for the period 1979 through 2007.** ***Note that 2008 fluxes are preliminary as they are based on provisional data.** ***** **Maximum**

**Average**

**Minimum**

Figure 15. Estimated nitrate delivery to the Gulf of Mexico for April, May, and June 1979 - 2008. Maximum, minimum, and average fluxes are determined through 2007. *Note that the 2008 deliveries are preliminary as they are based on provisional data. Source: Brent Aulenbach, USGS, http://toxics.usgs.gov/hypoxia/mississippi/oct_jun/graphics. html

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indicated above, the design limits their applicability to coastal assessments. USGS also monitors inland watersheds in selected major river systems that drain to the coast, using the same sampling design suitable for estimating annual nutrient fluxes. This monitoring is conducted in the MARB and in the Chesapeake Bay drainages. These data also are used to develop watershed models described in the following section of this report. Within the MARB, measurements are made at 19 inland stations. The delivery of streamflow and nutrients from the Mississippi River basin to the Gulf of Mexico for the first nine months of the water year (October through June) is reported each year in July to enable Gulf scientists to analyze and forecast the size of the hypoxic zone in the northern Gulf of Mexico (Figure 10b). The hypoxic zone is estimated each year in late summer. In addition, a summary of nutrient loads for all Mississippi River stations for the period of record through 2006 is available on the Internet (http://toxics.usgs.gov/ hypoxia/mississippi/nutrient_flux_yield_est.html). This dataset is updated annually (Figure 15). 3.3.2. Modeling Nutrient Sources, Fate, and Transport Watershed and nutrient transport, yield, and flux modeling efforts are being undertaken ( EPA, NOAA, USDA, USGS ) to identify and manage the most significant nutrient sources within watersheds draining to coastal areas experiencing hypoxia. These modeling efforts include the SPAtially Referenced Regressions On Watershed Attributes (SPARROW) Model (http://water.usgs.gov/nawqa/ sparrow/), the Soil and Water Assessment Tool (SWAT) Model (http://www.brc.tamus.edu/swat/), and the Regional Nutrient Management Model (ReNuMa, Hong and Swaney 2007; http://www. eeb.cornell.edu/biogeo/nanc/usda/renuma.htm). The SPARROW model (Box 8) is a hybrid mechanistic/empirical, basin-scale simulation model developed by the USGS (Smith et al. 1997). It was developed to cover the entire conterminous United States. Since then, applications have been developed for watersheds of a wide range of scales, with data input refinements appropriate for the specific scale of application. It has been 

## Box 8. Application of SPARROW for Reducing Nutrients to the Gulf of Mexico

An improved SPARROW model published in 2008 (Alexander et al. 2008, USGS) found that much of the nitrogen and phosphorus delivered to the Gulf originates from certain regions and watersheds of the Mississippi River Basin, including many watersheds in the Central Mississippi and Ohio, which contribute more than 50% of the nutrients despite accounting for less than 30% of the total drainage area (see inset). The study reveals new details about sources of phosphorus, indicating that animal manure on pasture and range lands contributes nearly as much phosphorus as cultivated crops, 37% versus 43%, respectively

. The study reports that 66% of nitrogen originates primarily from cultivated crops, mostly corn and soybean, with animal grazing and manure contributing only about 5%. Atmospheric contributions also are important, accounting for 16% of nitrogen. These findings influenced the 2008 decision by the Mississippi River/Gulf of Mexico W atershed Nutrient Task Force to call for reductions in phosphorus, in addition to nitrogen, in an effort to control Gulf hypoxia.

Percentage of stream nutrient load delivered to the Gulf of Mexico: (a): total nitrogen; (b) total phosphorus. (Alexander et al. 2008).



### a) total nitrogen b) total phosphorus

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## Federal Hypoxia and Watershed Science Research

used to estimate nutrient loads and concentrations in streams and to determine the proportion of stream loads that are derived from major sources of nutrients, including land use, chemical use, and human activities (e.g., agriculture, atmospheric deposition, human wastes). The model accounts for the effects of climate, topography, soils, and the effects of aquatic ecosystems to determine nutrient transport in watersheds. Formal estimates of uncertainties in the stream nutrient loads and source contributions are also reported. The model has been applied to small and very large watersheds in the United States and internationally to assess nutrient sources and loads to the Mississippi River basin (Alexander et al. 2000, 2008), New England watersheds (Moore et al. 2004), the Chesapeake Bay watershed (Preston and Brakebill 1999), New Zealand river basins (Alexander et al. 2002; Elliott et al. 2005), and North Carolina coastal watersheds (McMahon et al. 2003). Many of these applications have demonstrated the particularutility of the model for quantifying the longdistance transport and delivery of nutrients to sensitive downstream locations such as estuaries. Federal and state environmental managers have used the SPARROW model to assess nutrient sources in streams, including its use for targeting nutrient reduction strategies in the Chesapeake Bay watershed (Preston and Brakebill, 1999) and in waters of the State of Kansas (Kansas Department of Health and Environment, 2004), and for developing total maximum daily loads (TMDLs) in the Connecticut River basin (NEIWPCC, 2004). Regional nutrient models are currently under development in selected regions with the goal of refining the national SPARROW model as part of the USGS National Water Quality Assessment (NAWQA) Program. The SWAT model was developed by the USDA Agricultural Research Service (ARS; Neitsch et al. 2004; Arnold et al. 1998). It is a physically based, deterministic, continuous, watershed simulation model. It predicts water, sediment, nutrient, and pesticide yields using spatial data on topography, soils, land cover, land management, and weather. The SWAT model has been applied in the Mississippi River basin, including for large watershed-scale applications (Arnold et al. 1999; Anand et al. 2007), to evaluate agricultural nutrient reduction strategies (Santhi et al. 2001; Vache et al. 2002; Hu et al. 2007), address tile-drained


## Box 9. EPA Works Closely With States to Develop and Adopt Nutrient Criteria

• 

### Direct assistance to states close to adopting criteria, particularly permitting implementation questions. In this regard, EPA’s Office of Science and Technology (OST) in the Office of Water works to develop tools to help states calculate the economic and ecological benefits of adopting water quality standards. OST has provided scientific peer review of proposed water quality standards packages for Vermont and draft nutrient criteria for Colorado. A cooperative assistance grant from EPA’s Gulf of Mexico Program to the Mississippi Department of Environmental Quality is assisting MS in all aspects of nutrient criteria development. MS will then lead the other Gulf of Mexico states in nutrient criteria setting for their own states through the Gulf of Mexico Alliance initiative.

• 

### Building capacity of States gathering and analyzing data. Over the past three years, OST has provided $2.5 million to support state nutrient criteria work. In 2008, EP A

is analyzing nine nutrient data sets that will be used for nutrient criteria. EPA OST also developed a number of webcasts that provided expert technical guidance on nutrient criteria issues, with examples from states that have successfully developed nutrient criteria.

• 

### Building a science-based foundation for developing new Clean Water Act water quality criteria for estuaries, wetlands and Large Rivers: OST published a wetlands criteria technical guidance manual, developed case studies for two estuarine criteria efforts (Yaquina Bay, Oregon and Pensacola Bay, Florida) in collaboration with EPA’s Office of Research and Development, and are supporting four states (Maine, New Hampshire, Florida, and California) who are making progress on estuarine criteria. OST is also supporting nutrient criteria development efforts on several large rivers: the Big Sioux (South Dakota), the Missouri River, the Red River, and the Ohio River.

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cropland (Du et al. 2006; Green et al. 2006), assess the impacts of climate change on nutrient export (Jha et al. 2006), and predict stream flow processes of a forested watershed in coastal South Carolina (Amatya et al. 2008). A study comparing SWAT and a statistical approach based on SPARROW in the United Kingdom (Grizzetti et al. 2005) suggested using SPARROW as a screening tool for identifying sources, and SWAT for testing management practice scenarios, but found that both models were useful for estimating nitrogen loading in aquatic environments. Similarly, ARS and the NRCS of USDA have developed a system of computer models, the Agricultural Non-Point Source Pollution Model, to predict nonpoint source pollutant loadings in agricultural watersheds. This model contains a continuous simulation surface runoff model designed to assist in developing BMPs and TMDLs, as well as risk and cost/benefit analyses.

The ReNuMA model is a watershed model that enables the examination of various scenarios for reducing nitrogen losses from the landscape ( EPA ). Components of the model incorporate biogeochemical complexities and the impact of varying management practices on reducing nutrient loads. Continual development of the model has included an improved ability to incorporate atmospheric deposition and improve characterization of on-field nutrient management techniques to allow for more accurate simulation of varying management scenarios ( NOAA ). 

# 3.4. Assessing, Managing, and Reducing Nutrient Inputs to Coastal Ecosystems This section discusses tools or management measures that are being studied and/or used to control or reduce nutrient delivery to coastal ecosystems. Nutrient fluxes to coastal ecosystems can primarily be reduced in two ways: (1) reducing nutrient inputs to rivers and streams, including losses of nutrients from farmlands and loads from point sources, urban and suburban nonpoint sources, and atmospheric deposition; and (2) restoring and enhancing natural denitrification and nutrient retention processes via restoration of riverine and coastal wetlands and in some areas, diversions of stream waters to coastal wetlands.
3.4.1. Reducing Nutrient Inputs to Rivers and Streams
3.4.1.1. Establishing Nutrient Criteria


### The Clean Water Act directs states and tribes to designate uses for their waters and adopt water quality criteria to protect those uses. Numeric nutrient standards are an important component to protecting water quality from excessive algal blooms and hypoxia because they drive water quality assessments and watershed management, facilitate development of TMDLs, provide quantitative targets to support nutrient trading programs, facilitate writing protective National Pollutant Discharge Elimination System permits, provide increased effectiveness in evaluating success of nutrient runoff minimization programs, and provide objective water quality baselines against which to measure environmental progress. Nutrient criteria benefit not only the local waters in which actions are focused, but also the receiving coastal waters. EPA ’s Office of Science and Technology (OST) in the Office of Water (OW) is providing both technical and financial assistance to states to help them develop and adopt numeric nutrient standards (Box 9).

Figure 16. Percent of the United States drained by artificial means such as tile drains (Jaynes and James 2007).

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## Federal Hypoxia and Watershed Science Research

from many local, state, and Federal agencies, including EPA and USGS . The mission of the ADMS Task Force is to reduce the loss of nitrogen and phosphorus from agricultural lands through drainage water management. The Task Force focuses on an eight-state region—comprised of Minnesota, Wisconsin, Iowa, Missouri, Illinois, Michigan, Indiana, and Ohio—that contains morethan 50 million acres of surface- and subsurfacedrained cropland. The Task Force works with farmers, contractors, and agricultural advisors to: 1) implement improved agricultural surface and subsurface drainage in both new and retrofitted systems; 2) reduce nitrate loads in drain outflow, a major source of poor stream water quality and hypoxia in the northern Gulf of Mexico; and 3) improve the efficiency of production and economic returns through managed surface and subsurface agricultural drainage. Implementation of managed drainage practices (NRCS Practice 554) in the Midwest began in 2004. Using demonstration sites, the Task Force educates local sponsors and farmers and works with them to implement managed drainage on their own land (http:// 3.4.1.2. Tools and Management Strategy Development for Reducing Nutrient Loadings 

### Reducing nutrient loadings to streams and rivers surrounding and downstream from agricultural lands has been an active area of research for USDA . The re-engineering of the Midwest over the past 50 years with tile drainage systems that allow farmers to control subsurface water levels has benefitted U.S. agriculture through increased yields, but has negatively affected water quality by speeding water and its solutes—such as nitrogen, phosphorus, pesticides, and sediment—into streams and rivers without allowing natural elimination processes to occur (Jaynes and James 2007) (Figure 16). As a result, USDA has made significant investments in conservation programs that remove marginal land from production, conservation improvements in working lands that reduce erosion and control nutrients, and the establishment of wetlands. In addition, USDA has worked with farmers and ranchers to encourage the use of buffer strips and improved tillage practices that are designed to prevent water quality impairments. Since nutrients often leave agricultural fields through drainage systems (e.g., tile drains, ditches), significant research by the USDA has focused on fine-tuning both surface and subsurface drainage applications (Box 10). In the Chesapeake Bay watershed, it has been estimated that nitrogen losses could be reduced by 40% by lowering the water table during planting and harvesting. Other modified surface drainage systems have the potential to reduce phosphorus by 30-50%, and nitrate and ammonium by 22-64%. In the Gulf of Mexico watershed, researchers have discovered that planting cool-season perennial forages such as alfalfa and grass directly over subsurface tile drains removes nitrate from the water table. Other options being explored include deep chiseling and the use of wood chips in drainage ditches to promote denitrification. Additionally, in 2003, NRCS and ARS ( USDA ) formed the Agricultural Drainage Management Systems (ADMS) Task Force, in concert with university research and extension personnel. The task force has since expanded to include scientists 

## Box 10. Soil Drainage Research in Ohio

Scientists at USDA ARS’ Soil Drainage Research Unit in Columbus, Ohio, have developed an innovative agricultural water management system called a Wetland Reservoir Subirrigation System (WRSIS), comprised of a wetland and a water storage reservoir linked to a network of subsurface pipes used at different times to either drain or irrigate crops through the root zone. Runoff and subsurface drainage are collected from cropland into a constructed wetland. Natural processes in the wetland treat the water by removing some of the nutrients, pesticides, and sediment. The water is then routed to a storage reservoir and held until needed to subirrigate the crops during dry parts of the growing season. The storage reservoir also provides a further opportunity for sediment and adsorbed nutrients to settle out of the water. Most of the time, a WRSIS operates as a closed loop, thus restricting offsite water release. Benefits of these systems include: (1) enhanced crop yields; (2) reduced offsite release of nutrients, pesticides, and sediment; (3) increased wetland vegetation and wildlife habitat; (4) carbon sequestration in soil; and (5) the potential to decrease flooding downstream (http://www.ars.usda.gov/ Research/docs.htm?docid=14999).

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extension.osu.edu/~usdasdru/ADMS/ADMSindex. htm).

USDA has invested in developing a variety of strategies and technologies for more effective management of fertilizer applications. USDA developed the Nitrate Leaching and Economic Analysis Package model to evaluate the sensitivity of different regions to nitrate loss. USDA ARS developed the Phosphorus Index, an assessment tool that has been adopted by USDA NRCS, EPA
, and various state agencies as the basis for their comprehensive phosphorus management plans. Adoption of this technology has been estimated to reduce annual phosphorus loadings in water by 56 million pounds and sediment by 2.1 billion pounds. Economic benefits to society have been estimated at greater than $600 million dollars per year.

Considerable research has also focused on developing sensors to detect the water and nitrogen status of crops, which allows growers to target fertilizer and water applications to specific crop needs. By reducing unnecessary fertilizer applications, this approach is also more economical. The sensors, some of which have been developed through the Small Business Innovation Research Program grants to private industry, can be either ground-based or airborne. Ground-based sensors can be mounted on central pivot or other automatic irrigation systems. Researchers are also exploring how to reduce nitrogen and phosphorus release from fertilizers. The benefits of crop rotation, specifically the planting of soy before corn, are being used to justify applying less chemical fertilizer to corn since planting soybeans builds up a nitrogen credit. Farmers can save up to $30 per hectare with this approach. Finally, cover crops are a proven technology for the eastern and southern states, but are still being researched and introduced to Midwest farmers. Cover crops (Box 11) may be the best technology for simultaneously reducing nitrogen, phosphorus, and sediment from row crops. Further, USDA ’s Conservation Reserve Program (CRP) is a voluntary program available to agricultural producers that protects environmentally sensitiveland through planting of resource-conserving, longterm vegetative covers (Box 12).

Based on a rich dataset of observations and an improved, process-based simulation model (called the Root Zone Water Quality Model or RZWQM), ARS scientists have summarized and quantified the effects of management on nitrogen losses to surface waters through a series of papers published as a special issue of the scientific journal Geoderma (Ahuja and Hatfield 2007). Both water and nitrogen losses from tile-drained agricultural systems in the Midwest are addressed, as influenced by a variety of management practices. This special issue is a key resource for future efforts to control the magnitude and extent of the hypoxic zone in the Gulf of Mexico by reducing nitrogen loadings from tile-drained agriculture in the Mississippi River basin. Also, USDA ’s Forest Service is developing practices to improve the capacity of nutrient-enriched watersheds to reduce nutrient loads in surface water and groundwater before they enter coastal waterbodies. For example, the creation of “hot spots,” places in a Figure 17. 2007 Map of CEAP projects, many of which are focused on the Mississippi River Watershed (http://www.nrcs.usda.gov/technical/nri/ceap/).

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## Federal Hypoxia and Watershed Science Research

ARS completed development of the Sustaining the Earth’s Watersheds Through Research, Data Analysis, and Synthesis (STEWARDS) database— an agency-supported repository for CEAP datasets collected as part of ARS’ Benchmark Watershed Research Network (available at http:// arsagsoftware.ars.usda.gov/stewards/).
3.4.2. Restoring and Enhancing Natural Nutrient Retention Processes to Reduce Pollution of Aquatic Environments Nutrient fluxes to coastal ecosystems can be reduced when nutrients already in stream systems are removed by natural processes that occur in riverine wetlands, certain types of reaches of watershed where denitrification could be enhanced, was shown to be an effective way to reduce the amount of nitrogen leaving urban watersheds (Strosneider et al. 2007, Pouyat et al. 2007), resulting in reduced nutrient loading of coastal
waters. In late 2002, the Office of Management and Budget asked NRCS ( USDA ) to quantify the benefits of Farm Bill-funded conservation programs. In 2003, the Conservation Effects Assessment Project (CEAP) (Figure 17, Box 11) was initiated by NRCS along with other USDA agencies (ARS; Cooperative State Research, Education, and Extension Service or CSREES; and FSA) to improve scientific understanding of the effects of conservation practices at the watershed scale, and to estimate conservation effects for reporting at regional and national scales. The results are being used to manage agricultural landscapes for environmental quality. CEAP has grown into a USDA -led multi-agency, multiresource effort (Maresch et al. 2008). Duriancik et al. (2008) provide a detailed description of the approach of CEAP, and of accomplishments during its first five years. Specific details of ARS research accomplishments during the first five years of CEAP are collected in a special issue of the Journal of Soil and Water Conservation (2008). 

## Box 11. Monitoring Winter Cover Crop Performance from Space

Planting winter cover crops is seen as an important management practice for reducing agricultural nutrient flows into the Chesapeake Bay and improving ecosystem health. The State of Maryland doubled its budget for its cover crops cost share program from $9 million to $18 million in 2008. This was enough for farmers to plant 500,000 acres of cover crops after the fall 2008 harvest. USDA scientists at the Beltsville Agricultural Research Center have formed a strong partnership with the Maryland Department of Agriculture (MDA) for the evaluation of winter cover crop performance on the Eastern Shore of Maryland which is closely linked to the Bay. The evaluation uses a unique combination of remotely sensed data and program implementation information such as field location, planting date, cover crop type, and planting method, all supplied by participant farmers during program enrollment. Based on this information, detailed evaluation of cover crop program performance for growth and nutrient uptake can be obtained for all enrolled cropland fields within a region based on analysis of satellite images. This powerful new approach has led to improvements in the operation of the MDA program for cover crops. It has also demonstrated how satellite data can be used routinely to implement and monitor this important state conservation program. There are plans to jointly develop an operational cover crop implementation and monitoring tool based on technologies developed by this collaboration. This research effort is part of the Choptank River Watershed CEAP project. Photo: Ben Longstaff, www.eco-check.org



## Box 12. USDA Conservation Research Program Benefits in the Mississippi River Basin

In the Mississippi River Basin, the CRP presence is estimated to confer the following benefits annually:

• Reduced nitrogen runoff: 295 million pounds

• Reduced phosphorus runoff: 66 million pounds

• Reduced sedimentation: 100 million tons

• Protected/restored wetlands: 1.2 million acres

• Carbon dioxide sequestered: 12 million metric tons

• Enhanced wildlife habitat

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## Chapter 3

river systems, and coastal/distributary wetlands. Existing and proposed freshwater diversions of the Mississippi River into the estuaries of southeastern Louisiana to mitigate land loss (from many factors including subsidence, salinity intrusion, etc.) have the ancillary benefit of removing nutrients.

The USGS SPARROW model was used to demonstrate that stream channel geometry can affect nitrogen removal in streams in the Mississippi River basin (Alexander et al. 2000). Small streams were found to have a significantly higher nitrogen removal rate, while larger streams acted more like conveyors, transporting the nitrogen load downstream. Therefore, the proximity of nitrogen inputs to larger streams is an important consideration for management actions because it influences whether the nitrogen in a stream will be carried to the Gulf of Mexico or removed. The Riparian Ecosystem Management Model (REMM) simulates hydrology, nutrient dynamics, and plant growth in land areas between the edge of fields and a waterbody. REMM output allows designers to develop buffer systems to help control nonpoint source pollution ( USDA ). Analysis of the optimal composition of riparian buffers for nutrient removal has led to various design criteria, including the recommendation that trees or forests be part of the buffer, and analysis of the effectiveness of various grass species as alternatives. The grass analysis determined that all grasses except timothy were beneficial for nutrient removal ( USDA ). Some of the natural processes that reduce nutrient fluxes to receiving waters include: 1) restoration of riverine wetlands by the U.S. Fish and Wildlife Service ( USFWS ) and USACE
, 2) management of river flows to increase conditions conducive to increased nutrient reductions, such as lock and dam management on the upper Mississippi River, and 3) coastal diversions onto the Mississippi River delta at projects like the Caernarvon Freshwater Diversion Project. Similarly, flow management on the Mississippi River that diverts 30% of flow down the Atchafalaya River provides increased opportunity for nutrient removal in extensive distributary wetlands in the lower reaches of that river. USDA created more than 12.1 million acres of new wetlands in 2005 and 2006 as a result of research showing that water leaving a wetland had only a fraction of the nutrients (18%) than in the subsurface water entering the wetlands from agricultural fields. The wetlands program within USDA CRP was augmented by the 2008 Food, Conservation, and Energy Act, with a specific emphasis on encouraging producers to construct wetlands to remove nitrogen before it enters
streams. The strategic integration of perennials into agro-ecosystems provides greater benefits than their spatial extent suggest (Schulte et al. 2006; USDA Forest Service). Current research at the field, watershed, and landscape scales in the upper Mississippi River basin is focused on several components that contribute to the mitigation of nutrient loading to the Mississippi River. Researchers have employed simulation models to extrapolate results from small-scale studies over broad scales. Also, research is being conducted on the funding and social mechanisms needed to work with private landowners because, to be successful, the research must be participatory. The USDA Forest Service’s Center for Bottomland Hardwood Research in Mississippi is a major partner in the effort to restore agricultural lands to bottomland hardwood ecosystems in the Mississippi Valley. An important part of the program is developing and delivering technology on how to design and install forested buffers to protect water quality.

The USDA Forest Service has conducted extensive research on reforestation restoration techniques, their costs, and expected benefits (Gardiner and Oliver 2005). The majority of reforestation projects in the Lower Mississippi Alluvial Valley (LMAV) use low intensity (less expensive, but less assured in obtaining desired benefits) methods that were established through the CRP and the Wetland Reserve Program (Gardiner and Oliver 2005). These programs provide subsidies for landowners willing to convert agricultural land to forest, range from ten year agreements to perpetual easements, and reimburse landowners for 75% to 100% of the

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## Federal Hypoxia and Watershed Science Research

costs of approved practices. By 2007, reforestation reclaimed an estimated 200,000 ha in the LMAV (Gardiner and Lockhart 2007) and 75% of this land is privately owned (Gardiner and Oliver 2005). A tool that will help achieve that goal is a decision support system that can identify the geographic areas with the greatest potential for restoration and provision of related ecosystem services. The Eco-Assessor, a decision support system developed by the USGS and EPA (Davis et al. 2002), can help planners and managers target areas with the greatest potential for nutrient removal from river water by increasing the weight of the most important functions in the model (water quality) and reducing the weight of less important functions (habitat, hydrology, and restorability). This can allow managers to target areas where the probability of restoration success is highest, and eliminate from consideration areas with less potential. This can sustain public support for wetland restoration by having the most accurate information available to the public. 

# 3.5. Economic Assessments
Determining the economic impacts of coastal hypoxia and the costs and benefits of nutrient reduction within watersheds has proven difficult and complex. However, some progress has been made and efforts are ongoing. Within the watershed, the USDA Economic Research Service (ERS) evaluated the economic costs of reducing nutrient loads to the Gulf of Mexico for the original Committee on Environment and Natural Resources (CENR) study (Doering et al. 1999). Since then, ERS has provided some additional insights on policy options for reducing nutrient loads to the Gulf. In 2001, ERS compared source reduction (fertilizer management) and interception strategies (wetland restoration) for controlling nitrogen loss in the Mississippi River basin (Ribaudo et al. 2001). ERS found that a standard on fertilizer applications was more cost-effective than restoring wetlands up to a particular level of total nitrogen loss reduction (about 26% reduction). Beyond this point, wetland restorations are more cost-effective. In 2005 ERS assessed the economic benefits of a water quality trading program between point and agricultural sources in the Mississippi River basin (Ribaudo et al. 2005). Allowing trades between point and agricultural sources was found to reduce overall nitrogen abatement costs. A geographically extensive trading program was also found to raise crop prices, increase production, erosion, and nutrient loss outside of the basin. Additionally, there are ongoing efforts to link the watershed models with the USDA REAP (Regional Environment and Agricultural Programming) economic model developed by USDA ( NOAA ). This linkage will allow for analyses of the economic costs of varying management scenarios and the corresponding benefits in reducing nutrient inputs As indicated in Section 3.3.2, limited knowledge of the ecological impacts of hypoxia on living resources has restricted analyses of the economic impacts of hypoxia to coastal communities. However, a recent bioeconomic model developed to examine hypoxia in the Neuse River estuary in North Carolina indicates the economic impacts of hypoxia may be high ( NOAA ). The model indicates that reductions in the spatial and temporal extent of hypoxia could increase profits to shrimpers, assuming no additional entry into the fishery was permitted. Preliminary estimates from this model have suggested that a 30% reduction in the number of hypoxic days in the Neuse River estuary could potentially increase shrimper profits by $2.5 million annually. As knowledge of the population and physiological impacts of hypoxia grows, it is anticipated that the economic consequences will become clearer.

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# Future Research Directions **and Interagency Coordination** Chapter 4

4.1. Introduction 

# F ederal research programs are addressing many aspects of eutrophication, nutrient enrichment and hypoxia. Despite decades of research, however, management efforts to reduce nutrients—particularly from nonpoint sources—and their adverse impacts on coastal ecosystems have not made significant headway, in part due to increased development and population in coastal watersheds. The task of managing nutrients and associated hypoxia within and across diverse landscapes and political jurisdictions is complicated by a growing, but still incomplete, understanding of the status of eutrophication of coastal ecosystems and the processes that control water quality responses to excess nutrients. But there are significant opportunities for advancement. Scientific data and tools are, as of yet, inadequate to fully inform management actions directed at hypoxia in all of the coastal environments impacted by hypoxia. Ideally, such tools would allow managers to readily establish reasonable science-based goals by quantitatively linking management actions to nutrient loads, nutrient loads to coastal water quality, and coastal water quality to ecological outcomes of concern. This chapter brings together conclusions of a number of reports that independently address research endeavors that could best improve management of hypoxia in U.S. coastal waters. These reports resulted from deliberative, inclusive, and cooperative efforts or workshops focused on determining research priorities. Research priorities related to hypoxia in the northern Gulf of Mexico have received more concerted Federal deliberation recently than priorities for other systems and have been addressed in at least five major reports. These include 1) the 2008 Gulf Hypoxia Action Plan (Mississippi River/Gulf of Mexico Watershed Nutrient Task Force 2008), 2) the Hypoxia in the Northern Gulf of Mexico, An Update by the EPA Science Advisory Board (U.S. EPA 2007)

, 3) the Gulf of Mexico Hypoxia Monitoring Implementation Plan (Gulf of Mexico Hypoxia Monitoring Implementation Plan Steering Committee 2009), 4) the Science Strategy to Support Management Decisions Related to Hypoxia in the Northern Gulf of Mexico and Excess Nutrients in the Mississippi River Basin (Mississippi River/Gulf of Mexico Watershed Nutrient Task Force 2004), and 5) Nutrient Control Actions for Improving Water Quality in the Mississippi River Basin and Northern Gulf of Mexico (NRC 2008). The Lake Erie Research Planning Workshop Report (NOAA 2004) addressed research needs for Lake Erie, including priorities for addressing hypoxia. The introduction to the special issue of the Journal of Experimental Marine Biology and Ecology on the Ecological Impacts of Hypoxia on Living Resources (Kidwell et al. 2009) provides a framework for guiding research aimed at assessing the effects of hypoxia on living resources. Broad guidance regarding research priorities has been suggested by national assessments such as the National Estuarine Eutrophication Assessment (Bricker et al. 2007). Although research is clearly being done to support management of eutrophication and hypoxia in systems, such as Chesapeake Bay, Long Island Sound, Narragansett Bay, and Hood Canal, recent documentation of an overall research strategy for these coastal ecosystems does not exist.

Some of the identified research needs are common across coastal systems or regions of the country, but there are also differences. These differences result from variability in physical and ecological properties and in the research carried

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out to date. Four main categories of research needs are identified in this report and discussed below. Within each category, system-specific or regional differences in research needs are examined. 

# 4.2. Improved Characterization and Quantification of Hypoxia
Effective management of hypoxia requires that the causes, extent, and severity of hypoxia be adequately understood and quantified. If the causes are not thoroughly understood, effective management actions cannot be formulated. Adequate monitoring information is required to know the scope of the hypoxia problem and to understand if hypoxia is expanding or being reduced as a result of management actions. Adequate quantification of hypoxia is needed for individual coastal ecosystems that experience recurrent and severe hypoxia (e.g., the northern Gulf of Mexico) and for the Nation’s coastal waters as a whole. Causes of hypoxia are relatively well understood in some systems (e.g., Chesapeake Bay, Gulf of Mexico, Long Island Sound), but there are many other U.S. systems that have only relatively recently been identified as experiencing hypoxia. The general phenomena of transient hypoxia due to diel-cycling of dissolved oxygen levels can have dramatic consequences, such as fish kills, because of its frequency and location of occurrence. This phenomenon is probably more widespread and problematic than currently documented. Process studies are needed to elucidate system dynamics and clarify causes. For example, improved understanding of how nutrient and sediment dynamics contribute to hypoxia will lead to better predictive models. Further, it is currently hypothesized that some systems have experienced regime shifts (e.g., Gulf of Mexico, Chesapeake Bay) due to nutrient overloading which has the effect of increasing the amount of hypoxia for a given load of nutrients. Determining factors underlying such changes is important for refining models and management actions. Hypoxia models provide the ability to test hypotheses about the causes (i.e., nutrient levels, freshwater flow, stratification, sediment dynamics, etc.). Further model development and transfer to operational use by coastal managers should be pursued.
Monitoring of hypoxia in U.S. coastal waters is currently imbalanced, with some systems monitored very well (e.g., Chesapeake Bay) and others monitored much less frequently. In some cases, a significant effort has been expended, but substantial gaps in knowledge remain because the affected area is very large and costly to sample adequately (e.g., Gulf of Mexico hypoxic zone). Monitoring strategies have often been inadequate to characterize the incidence of hypoxia because time or space scales for sampling are too broad. Further, in some waters, hypoxia occurs periodically and primarily (or exclusively) at night and cannot be captured with regular boat surveys during the day.

4.2.1. Hypoxia Surveys Hypoxia is often difficult to quantify since there is usually considerable temporal and spatial variability. Multiple surveys within a season are usually required to assess goals or standard in a scientifically valid manner and are critical for measuring progress and supporting long-term adaptive management strategies. For example, the extent of hypoxia in the Chesapeake Bay mainstem is monitored by Maryland, Virginia, and EPA at least monthly throughout the summer. This sampling design allows for an accurate assessment of the extent of the problem and has helped managers set dissolved oxygen criteria for the Bay and track progress toward meeting those criteria.In the northern Gulf of Mexico, however, one midsummer shelf-wide survey is conducted by the Louisiana Universities Marine Consortium as part of NOAA’s NGOMEX research program. Given the considerable variability of hypoxia during the summer in this region, one cruise does not provide the coverage needed to monitor progress toward the hypoxia reduction goal. Regularly scheduled shelf-wide monitoring of the northern Gulf of Mexico during the warmer months of April through September would provide the rigorous quantification of hypoxia that is required to assess the goal on an annual basis. This need has been documented in response to a summit that was held

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## Future Research and Coordination

to address the current deficiencies in monitoring the hypoxic zone in the northern Gulf of Mexico (Gulf of Mexico Hypoxia Monitoring Implementation Plan Steering Committee 2009). Although the Chesapeake Bay and some other systems (e.g., Long Island Sound) have been relatively well-sampled, there is no national program or strategy to systematically assess the extent, duration, frequency, or intensity of hypoxia in U.S. coastal waters. A survey program or coordinated monitoring network designed with an appreciation of the temporal dynamics and likely spatial distribution of hypoxia would provide a more complete representation of the extent of hypoxia nationwide.
4.2.2. Instrumented Observing Systems Ship-based surveys cannot reasonably address the extent of hypoxia in all U.S. coastal waters due to cost and, particularly, because of the temporal dynamics of hypoxia in some coastal systems. This challenge can likely be met by new technologies for measuring dissolved oxygen, particularly optical sensors, that can be deployed in the field to provide continuous measurements. Sensors are increasingly stable in their calibrations and resistance to biofouling. Their data can be relayed to investigators in near real-time via wireless data networks and other telemetry technologies. Instrumented observing systems require an initialinfrastructure investment, but then may offer costsavings relative to traditional surveys. Moreover, only continuously deployed instruments offer a practical means of characterizing temporal dynamics of hypoxia over a range of time scales, including diel-cycling hypoxia. Finally, in situ observing in the Gulf of Mexico presents special consideration. The frequency of hurricanes makes maintaining instrumented observing systems expensive due to the high cost of insurance and replacement costs when devices are lost in major storms; such expense is not feasible for most academic researchers. Only a Federal partnership with state and regional entities is likely to succeed in establishing and maintaining a comprehensive national monitoring capability for hypoxia.
4.2.3. Other Advanced Technologies Autonomous underwater vehicles, such as gliders and powered underwater vehicles, can carry sensor payloads and provide valuable information on the spatial distribution of hypoxia and related water quality variables. Like moored instrumented observing systems, they may generate cost savings by automating sampling and reducing the need for costly ship and boat operations. Hypoxia cannot be monitored directly via satellite remote sensing, but greater use of remote sensing could provide important data on factors that cause or control hypoxia, including chlorophyll- a , water clarity, suspended solids, and potentially other variables. Integration of adequate real-time data streams into operational nowcast-forecast simulation modeling systems (e.g., NOAA PORTS models in Mobile Bay, Narragansett Bay, and elsewhere) has the potential to provide both spatially and temporally An important tool for oceanographic research and water quality sampling - the CTD rosette. Photo: EPA Gulf Ecology Division

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## Chapter 4

resolved characterizations of hypoxia in coastal waters with reasonable and quantifiable accuracy.
4.2.4. Improved Modeling of Hypoxia Because hypoxia results from interactions between physical and biological processes in time and space, simulation models—especially coupled physical-biological water quality models—are especially useful. Scientists increasingly rely on models to test assumptions about the causes of hypoxia. In the past several decades, the technical difficulty, cost to implement, and computational requirements for running these models and evaluating their output have limited their use to a few relatively well-funded modeling programs, usually working in the highest profile areas (e.g., Chesapeake Bay). Recently, this situation has begun to change dramatically. Desktop computers today are vastly more powerful than they were a few years ago. Powerful parallel computing clusters can also be assembled at reasonable cost. Although the models remain complex, it is now possible for more investigators to consider implementing and using them. New and efficient open-source modeling codes such as ROMS (Shchepetkin and McWilliams 2005) and the Finite Volume Community Ocean Model (FVCOM) (Chen et al. 2006) offer powerful and advanced capabilities such as data assimilation, while also enabling relatively simple tests of concepts (e.g., Hetland and DiMarco 2008). The data needed to support the models can increasingly be obtained, sometimes in real-time or near real-time via internet data portals. It now seems possible that even reasonably complex models could be developed and used to support water quality management and other public needs in many coastal systems, or potentially the entire U.S. coast. Another consequence of the lower cost of implementing simulations models is that it is now possible and even desirable for several independent or “competing” modeling efforts to address similar issues for the same geographic location. This development is a positive one because scientific debate and consensus-building—which promote scientific advancement—cannot occur effectively if there is only a single modeling program. As an example, scientists working to address hypoxia in the northern Gulf of Mexico clearly indicated their preference for using a variety of different models relying on different approaches and assumptions (Mississippi River/Gulf of Mexico Watershed Nutrient Task Force 2004). The Federal government should continue to support modeling efforts by supporting open-source model code development, programs that maintain large-scale modeling efforts, and programs that provide data products that facilitate development of local-scale water quality models. 

# 4.3. Improved Characterization of Impacts of Hypoxia
Concern with hypoxia stems from the associated loss of human and aquatic life uses in affected coastal systems. To set reasonable objectives for management of hypoxia, and to justify and sustain efforts to achieve those objectives, it is important to quantify the impacts, both in terms of aquatic life use and ecosystem services provided to humans. Further, it is important to quantify losses that have or are presently occurring, those that will result from a further increase in hypoxia, and those ecosystem services that could be restored by reducing hypoxia. A known continuum of hypoxia effects has been empirically related to dissolved oxygen concentrations (e.g., Diaz and Rosenberg 1995; Kidwell et al. 2009), providing guidance on likely effects of hypoxia, even in the absence of direct studies of effects in a particular coastal system. However, direct and systematic assessments of ecological effects of hypoxia in coastal ecosystems, which are important for accurately evaluating impacts, are relatively rare. Dramatic and visible fish kills associated with hypoxia can garner significant public attention, but more serious effects are often much less obvious, such as reduced production. In systems subject to increases in hypoxia over time, the public and even scientists can fail to recognize both what has been lost and what could be regained by restoring water quality.

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## Future Research and Coordination

4.3.1. Impacts Surveys Priority research needs related to impacts of hypoxia in coastal waters focus on: 1) characterizing current and past biological condition in ecosystems affected by hypoxia 2) characterizing chronic, sub-lethal effects on individuals, 3) quantifying effects at the population and community level, and 4) characterizing interactions between hypoxia and other stressors. Characterizing biological condition of coastal ecosystems, such as via faunal assessments, is one of the most direct ways to evaluate ecological impacts due to hypoxia. Improved faunal monitoring presents an opportunity to increase our understanding. In the northern Gulf of Mexico, NOAA SEAMAP conducts trawl surveys to assess fish abundance, but does not examine benthic community condition. In some less-studied systems where hypoxia has been documented (e.g., Pensacola Bay, Florida), very little data are available to characterize biological condition, and no substantial attempt has been made to relate condition to the extent of hypoxia. These data limitations contrast with more extensive studies in the Chesapeake Bay system, where decades of benthic and fish surveys describe the effects of hypoxia.
4.3.2. Assessing Biological Responses and Modeling Models are invaluable tools for quantifying the effects of hypoxia, sometimes providing insights into biotic effects of hypoxia that would be impossible to resolve from empirical observations alone. Studies that provide a detailed understanding of the effects of low oxygen on biota present an opportunity to better support these models and increase what can be learned from them. For example, motile species can sometimes avoid exposure to hypoxia by moving away. Species may also modify feeding and other behaviors, impacting growth, reproduction, and often predator avoidance. Further research describing these behaviors would support better models to predict effects of hypoxia on individuals, populations, and communities. Quantification of shifts in energy flows within entire food webs as a result of eutrophication and hypoxia (e.g., Baird et al. 2004) would provide integrated perspectives on the effect of hypoxia on marine life in coastal systems. Unfortunately, hypoxia is rarely the only stressor in coastal systems. Therefore, better data and models are needed to examine hypoxia interactions with additional stressors such as fisheries exploitation, habitat loss, and invasive species. Finally, development of bio-economic models could help assess effects of hypoxia on economically important fisheries and other ecosystem services, thereby helping provide an improved understanding of the possible economic benefits from nutrient management.
4.3.3. Assessing Economic Impacts For accurate economic analyses, data are needed that account for the many sources of variability influencing population dynamics and landings. In addition, more knowledge on hypoxia occurrence and distribution as well as data on effects on various ecosystem components are needed. Similar limitations are encountered when trying to estimate economic losses from HABs (Anderson et al. 2000). Long-term monitoring programs that measure dissolved oxygen at proper spatial and temporal scales coupled with ecosystem models described in the previous section are critical for resolving the ecological impacts that then set the stage for assessing the economic impacts attributable to hypoxia. 

# 4.4. Quantifying Nutrient Flux to Coastal Waters
Freshwater inflow and nutrient inputs to coastal waters are the primary causes of eutrophication and hypoxia in coastal waters. These factors remain important areas of uncertainty for coastal science and management. Poor quantification of these factors makes it much harder to relate hypoxia and the associated water quality effects in coastal waters to the specific anthropogenic causes in their drainage basins. Tracking nutrients delivered to coastal waters back to source areas and human activities on the landscape is essential for designing effective management strategies to reduce nutrient losses within the drainage basins and to reduce nutrient delivery to coastal systems. Additionally,

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it is important to quantify the environmental processes that influence transport and fate of nutrients within basins as they affect ultimate delivery to coastal ecosystems.
4.4.1. Monitoring Nutrient Sources and Fluxes to Coastal Ecosystems Monitoring streamflow and nutrient fluxes theoretically is straight-forward. Load estimatescan be made using well-established regressionbased models (Cohn et al. 1992; Runkel et al. 2004) or related methods (Aulenbach and Hooper 2006) that rely on streamflow monitoring data and regular measures of water chemistry. However, the number of gauged rivers is small, and contributing drainage basins can extend over broad areas quite distant from the coast. As a result, significant monitoring is required to provide sufficient data to make adequate estimates of the delivery of streamflow and nutrients to the coast and the source areas and human activities within the basin that affect them. Although simple in theory, there remains room to improve the statistical methods of load estimation directed largely at reducing the frequency and costs associated with water quality monitoring. Nutrient load estimates with adequate temporal resolution to develop cause-and-effect linkages to the onset, extent, and duration of hypoxia and to support empirical and simulation models of coastal systems currently are not available in the United States. This is largely a result of insufficient frequency of water quality monitoring and inadequate streamflow gauging. Acceptably accurate estimates of loads are needed at monthly or higher frequencies (e.g., weekly). However, increased temporal resolution comes at the expense of increased limits of uncertainty unless there is a commensurate increase in the frequency of sampling. Continuous real-time nutrient monitoring stations offer a potential solution. These have been pioneered in a few locations such as the mouths of the Mississippi and Atchafalaya Rivers. However, the technology needs further development to be more practical and reliable and to provide data on a sufficient suite of water quality constituents. Monitoring riverine delivery of nutrients beyond tidal waters to coastal systems has been complicated by difficulties in stream gauging and representative sampling in tidal reaches. New techniques employing acoustic doppler current profilers have made these measurements possible and reduced the uncertainty associated with nutrient removal or additions in tidal river reaches (Box 13). Measurements of point source loadings of nutrients are also needed. For most coastal waters, these are not sufficiently accessible. The Chesapeake Bay Program’s Nutrient Point Source Database provides a good example of what should be implemented much more broadly. A consistent, systematic, and comprehensive nationwide approach for estimating nutrient loads to coastal waters from all upland sources (fluvial, diffuse, point source, atmospheric) would be a very valuable tool to support science and management related to hypoxia in U.S. coastal Dr. Peter Eldridge extracts sediment cores to investigate sediment biogeochemical processes in the Gulf of Mexico hypoxic zone. Photo: EPA Gulf Ecology Division

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## Future Research and Coordination

waters. Estimates for atmospheric deposition, both dry and wet, to coastal systems is a source of great uncertainty in the understanding of nitrogen and carbon cycling in watersheds.
4.4.2. Modeling Source Areas, Source Mechanisms, and Trends in Nutrient Loads Modeling is a valuable tool for interpreting data across a broad range of scales and employing a range of approaches from purely deterministic simulation modeling to statistically based empirical approaches. The interpretation of trends in streamflow and nutrient loads provides important feedback to adaptive management. Separation of natural variations from those induced by human activities, such as changes in land use practices and management actions to reduce nutrient loses, can be extremely challenging. New statistical models are needed to implement such time series analyses.

There are insufficient resources to monitor streamflow and nutrient loads at all inland watersheds. As a result, monitoring is done in watersheds representative of others with similar climate, land use, other measures of nutrient inputs, wetlands, and management actions. In turn, the models help identify the representative watersheds. The models then extend the monitoring data across the entire basin to show the different contributions geographically and to identify the relative contributions from different anthropogenic sources. Such spatial information should be considered when management actions are implemented so that resources are invested where they will have the greatest benefit. Although the SPARROW model has been applied at a national scale (Smith et al. 1997), as well as regional (e.g., Preston and Brakebill 1999, Moore et al. 2004) and smaller scales, one limitation of SPARROW is that it has been applied to estimate average annual loading. Other approaches are needed where seasonal or interannual differences must be resolved. Isolated implementation of models, such as Hydrological Simulation Program-Fortran, can meet this need, but it has not been developed for most of the Nation’s estuaries. Ancillary data on human activities across the landscape that affect nutrient inputs are critical to the development of models that extend monitoring data across large contributing drainage basins. These ancillary data include information on chemical use, land use, and information on the types and distribution of management actions intended to mitigate nutrient losses to streams. The data include type of crop or animal agriculture; the extent, pattern, and intensity of agricultural drainage; the extent of riverine ecosystems that affect instream nutrient concentrations; and improved estimates of direct nutrient inputs (atmospheric deposition and point source discharges). Furthermore, to make accurate projections of future trends, research is needed to improve understanding of the consequences of long-term drivers, such as climate change, changes in cropping systems, extent of tile drainage, and biofuels production. Groundwater also can provide a significant contribution of water and nutrients to coastal waterbodies. It is difficult to quantify these flows and loads. Although ground-water flow system models can improve estimates of these contributions, they are difficult and expensive to develop. Cost-effective models are needed to assess the relative role of ground water in coastal ecosystems and to quantify its contribution where significant.

4.4.3. Nutrient Processes in Watersheds Quantification of the biogeochemical processes that affect nutrient transport on the landscape and within streams will also support development of sound land and water quality management practices. These processes affect how nutrients are transported from lands with various land use practices (crop agriculture, animal agriculture, urban, suburban) to streams and include various components of the hydrologic cycle (runoff, groundwater seepage, tile drainage, and atmospheric transport). Biogeochemical processes determine nutrient transport and persistence downstream to receiving waters, including nutrient transformation and removal. Ecosystem types that are most effective at reducing instream nutrient

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loads and the characteristics that make them most efficient must be identified. These include riverine, estuarine, coastal, wetland, and delta ecosystems.

Research is needed on small watersheds to integrate understanding of the source of nutrients; the role of groundwater, surface waters, riparian zones and wetlands in the transport of nutrients to the stream; and the downstream persistence of nutrients. A better understanding is needed of the temporal and seasonal variation in nutrient fluxes and the time lags associated with changes in drivers (increased inputs or mitigation measures) and resulting changes in nutrient loads. The role of various environmental media (sediment and organic matter) in defining nutrient budgets is poorly understood. Improved approaches are needed to characterize water and nutrient balances for land areas to which mitigation measures may be applied to ensure that resources are invested in the highest priority areas and to areas where management actions will have the greatest effect on nutrient loads to receiving coastal waters. Most research on nutrient cycling in watersheds is driven largely by concerns for science-based management actions that improve local water quality conditions. Typically, this research is not driven by concerns for water quality effects on coastal ecosystems, which can be quite distant— both geographically and culturally. An important goal of research on nutrient processes that is pertinent to hypoxia should be to consider aspects that are important to downstream receiving waters. 

# 4.5. Approaches for Reducing Nutrient Inputs to Coastal Waters
This research is directed at developing improved management tools for mitigating excessive nutrient fluxes to coastal ecosystems. It has two major components: 1) reducing nutrient inputs – research to identify BMPs for reducing nutrient inputs to streams, including losses from agricultural lands, point source inputs, urban/suburban runoff, and atmospheric deposition; and 2) enhancing nutrient removal – research to identify the instream nutrient reductions taking place in wetland areas, including reconstructed wetlands, river reaches amenable to nutrient removal processes, constructed riverine wetlands, and coastal diversions. Process research on nutrient cycling and transport is fundamental to both of these components. Understanding where nutrients are entering the watershed and how mucheach region is contributing will lead to more costeffective targeting of nutrient reduction strategies.

4.5.1. Evaluation of Nutrient Reduction Strategies Evaluations of nutrient reduction strategies should continue in a deliberate and measurableway to promote the most efficient and costeffective management alternatives. CEAP (see Section 3.4.1.2) is already proving to be a good tool for this purpose and may warrant expansion to more regions of the United States. CEAP and other scientific assessments should be continued to evaluate the effectiveness of current nutrient reduction management techniques, while developing ways to maximize their effectiveness 

## Box 13. USGS Deployment of New Instruments to Measure Water Flow and Sediment Flux

USGS is now deploying acoustic doppler current profilers on a variety of platforms to monitor a range of hydrologic measurements and applications, including computing continuous records of streamflow for tidally or backwater affected streams, measuring velocity fields with high spatial and temporal resolution, and estimating suspended-sediment concentrations.

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## Future Research and Coordination

and improve implementation and maintenance, including market-based environmental stewardship programs. Other assessments which should improve nutrient reduction management include: 1) socioeconomic and bioeconomic assessments of alternative nutrient management scenarios through the development of integrated economic and watershed models; 2) more thorough evaluation of the efficacy of dual nutrient (nitrogen and phosphorous) control practices; and 3) evaluation of the most effective ways to market nutrient reduction strategies to farmers. Further, the importance of seasonality and nutrient form should be considered in nutrient reduction strategies. Finally, as the understanding of climate change impacts becomes more refined, this new knowledge needs to be applied to current nutrient reduction strategies.

4.5.2. Biofuels Research Research on biofuel life cycles is needed to assess the environmental effects, economics, and resource availability of biofuel feedstocks. Research has already shown fewer environmental impacts if cellulosic feedstocks are used instead of corn. Region-specific biofuel production systems should be encouraged to maximize productionwhile minimizing environmental impacts. “Regionspecific” implies a more localized approach to the type of feedstock used for biofuel distillation (e.g., considering what grows best where), thereby reducing distance between farms and biofuel plants and closing the loop on byproducts reuse (e.g., feeding corn mash to live stock).
4.5.3. Local Water Quality Concerns In inland states, there are inherent difficulties in developing an appreciation for the implications that nutrient inputs to rivers ultimately have on coastal ecosystems. Therefore, it is important to consider both local and regional water quality concerns in nutrient studies and management strategies. Studies should consider the implications of excess nutrients in local streams and estimate the costs in terms of both lost ecosystem services and the local and regional benefits associated with improving local water quality conditions. Excess nutrients in streams, rivers, and lakes can result in a plethora of problems, including unsightly algal blooms, fish kills, nitrate or ammonia toxicity, and contaminated drinking water. The safety standard (maximum contaminant level) for nitrate in drinking water established by the EPA is 10 mg/L (Ward et al. 2005). Nitrate levels at or above this level have been known to cause a potentially fatal blood disorder in infants under six months of age called methemoglobinemia or “blue-baby” syndrome. Therefore, when selecting nutrient management strategies, it is imperative to look for ‘win-win’ situations that improve both local and coastal water and habitat quality.
4.5.4. Nutrient Management Technology Development Given the development of new technologies, such as the use of remote sensing for new applications (which include assessment of crop nutrient needs), it is important to continue to develop nutrient management technologies (e.g., cover cropping, siting of BMPs, stormwater controls) to reduce nitrogen and phosphorus inputs to the coasts arising from both agriculture and urban/suburban sources. Achieving higher sequestration of applied nutrients by crops and reducing losses from soils without compromising agricultural yield should be one of the central goals of this research. In general, the science and management practices related to control of nonpoint nutrient sources is far behind that for point sources, such as wastewater treatment plants.
4.5.5. Improve Natural Nutrient Removal Processes Evaluation and exploration of methods to enhance natural processes that remove nutrients from streams and rivers should be a high priority. Naturally vegetated watersheds, and particularly those that contain wetlands, are highly effective at nutrient retention and are important in both improving and sustaining water quality. Research is needed on how to identify the most promising land areas and methods to restore the ecosystem services provided by vegetated watersheds and wetlands. Also, adaptation research is needed to increase the resiliency of forests, rangelands, and aquatic areas in an effort to mitigate the adverse

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impacts of climate change. As the understanding of ecosystem processes improves, methods to evaluate ecosystem services and to develop incentives for the public will be needed. 

# 4.6. Existing and Future Coordination
In the immediate future, there is a strong need for improved data access across agencies. Access to data is critical for model development. Coordinated, consolidated, and improved access to data, relevant to particular watersheds and to hypoxia in the coastal zone and for use by watershed and coastal zone managers, should be available across agencies and to the public. NOAA provides data access through the National Oceanographic Data Center (http://www.nodc. noaa.gov/) which requires all NOAA -funded researchers to deposit data within two years of project completion. EPA’s STOrage and RETrieval (STORET) data system provides the same function for EPA -funded research (http:// www.epa.gov/storet/about.html). USGS data are stored in the USGS National Water Information System, which has a website for public access to the data (http://waterdata.usgs.gov/nwis). EPA and USGS have made significant progress toward providing scientists and policy makers an easier way to integrate access to their large water quality databases. A common suite of web services allow for the automated sharing of water monitoring data via a common format and terms (http://qwwebservices.usgs.gov/). USDA ’s ARS has recently developed STEWARDS (http://arsagsoftware.ars.usda.gov/stewards/) as a repository for data collected as part of ARS’ watershed research network activities towards the CEAP croplands initiative. All of the agencies need to improve access and usability and provide an interface among their various databases. Growing cooperation among Federal agencies and among Federal, state, local, and tribal agencies has enhanced hypoxia monitoring and research capabilities. Most coastal regions with hypoxia problems in the United States now have well coordinated, multi-agency, multi-state management plans with informed, active science and technology advisory committees, many of which evolved from the National Estuary Program initiative. Some of these regional partnerships have existed for decades while others are just forming. For instance, the Chesapeake Bay Program (http:// www.chesapeakebay.net/), which was founded in 1983, engages a range of stakeholders and has been responsible for organizing real, tangible actions to improve water quality in the Bay. On the other hand, the Puget Sound Partnership, which was just formed in 2007, is a relatively new effort to address the worsening water quality resulting from development of the Puget Sound watershed. A recently released report (December 2008) from the National Research Council of the National Academies recommends the creation of a Mississippi River Basin Nutrient Control Implementation Initiative (NCII) to be established jointly within the USDA and EPA (http://www. nap.edu/catalog.php?record_id=12544). The NCII would focus on many of the research recommendations outlined in Section 4.6 (e.g.,pilot studies on nutrient reduction, analysis of costeffective nutrient reduction strategies, etc.), but more importantly it would solidify coordination between the two Federal agencies primarily responsible for improving water quality in freshwater U.S. systems. Ultimately, though, there is no national policy to confront the issues of eutrophication and hypoxia, and no single Federal agency is in charge of coordinating a Federal response. As evidenced in this report, coordinated efforts are required across at least four major agencies ( EPA
, NOAA
, USGS
, USDA , as well as others) in order to address both the impacts of hypoxia and its root causes. Currently, the IWG-4H provides some measure of communication and coordination about hypoxia across agencies. In addition, interagency communication happens through deliberations in other IWGs which address related issues, such as the sustainable development of biofuels.

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## Executive Summary References

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## Appendices



# Appendices

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# Appendix I: Federal Agency Hypoxia or **Hypoxia-related Research**
I.A. Department of Agriculture I.A.1. Agricultural Research Service

The Agricultural Research Service (ARS) is an in-house scientific research agency of the U.S. Department of Agriculture. ARS conducts research to develop and transfer solutions to agricultural problems of high national priority and disseminates information to: 1) ensure high-quality , safe food, and other agricultural products; 2) assess the nutritional needs of Americans; 3) sustain a competitive agricultural economy; 4) enhance the natural resource base and the environment; and 5) provide economic opportunities for rural citizens, communities, and society as a whole.

The Water Availability and Watershed Management National Program is part of Natural Resources and Sustainable Agricultural Systems, one of four broad areas for ARS research. The national program structure allows ARS to link USDA scientists in laboratories around the country to address research problems of local, regional, national, and international interest, such as hypoxia. http://www .ars.usda.gov/ research/programs/programs.htm?NP_CODE=211

The Conservation Effects Assessment Project (CEAP) provides the farming community, the conservation community, the general public, the Office of Management and Budget, legislators, and others involved with environmental policy issues, with an account of the environmental ef fects or benefits obtained from USDA conservation program expenditures. CEA P is jointly managed by ARS; the Cooperative State Research, Education, and Extension Service (CSREE S); Farm Service Agency (FSA); and Natural Resources Conservation Service (NRCS). http://www.nrcs.usda.gov/TECHNICAL/NRI/ceap/ STEWARDS (Sustaining the Earths Watersheds Agricultural Research Data System) was created to house data collected as part of CEAP assessment for croplands. This data system organizes and documents soil, water, climate, land-management, and socioeconomic data from multiple agricultural watersheds across the United States, allowing users to search, download, visualize, and explore data for research and conservation management purposes. The National Agricultural Library, Beltsville, MD, has developed a series of six CEAP-related bibliographic databases that are available to the public through their website: http://www.nal.usda.gov/wqic/. This dynamic relational database offers more than 5,200 citations of current research findings from around the globe to help scientists and the public understand the environmental effects and benefits of conservation practices implemented through various USDA conservation programs. http://arsagsoftware.ars.usda.gov/stewards/. 

# I.A.2. Cooperative State Research, Education, and Extension Service

CSREES provides Federal assis tance in the form of grants for research, education, and extension activities related to agriculture. The CSREES Water Program focuses on creating and disseminating knowledge that ensures a safe and reliable source of water to meet the needs for food, fiber , and ener gy production; human use and economic growth; and maintenance and protection of natural environmental systems and ecosystem services. CSREES’ unique niche is conducting research, education, and extension programs to protect and improve water resources in agricultural, rural, and urbanizing watersheds (including forest lands, rangelands, and croplands).

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CSREES has been a partner with ARS, NRCS, and FSA in the CEAP. Thirteen watershed projects were jointly funded by CSREES and NRCS to evaluate the effectiveness of conservation practices on water quality at the watershed scale. These 13 watershed projects serve as examples of collaborative work between land grant universities and NRCS. The projects are unique in that they combine evaluation of the biophysical effects of conservation practices and the socioeconomic context of the watershed location. The watershed projects also combine research and extension/outreach activities – involving agricultural producers in project outcomes. Through CEAP, the land grant university system has developed substantial capacity to increase the understanding of ef fects of conservation practices and the effectiveness of conservation programs.CSREES and NRCS collaborated to fund two projects to synthesize results from the 13 watershedscale projects. These two projects will build a knowledge base to evaluate impacts of conservation practices over broad regions, improve management of agricultural landscapes, and inform policy decisions at the local, state, and national scale. The two projects differ in their approach – the first uses a synoptic overview of the 13 watershed studies and the second uses a modeling framework to spatially distribute results from the 13 watershed to broader geographic regions.CSREES also provides funding for a national network of regional projects that focus on performancebased programs to address water resource issues. The ten regional projects are geographically aligned with U.S. EPA regions (see www.usawaterquality.org). These regional projects are directly involved with addressing hypoxia through research, education, and extension programs on animal manure management, nutrient management, and social and economic policy sciences. They make research, education, and extension resources of the university system more accessible to Federal, State, and local water resources improvement efforts. Through these projects, new or expanded opportunities develop for agricultural producers and agriculturally impacted communities to adopt voluntary approaches for the improvement of water resources. They 

## promote delivery of multi-state programming to address regional and/or multiregional water quality concerns

. 

# I.A.3. Farm Service Agency The primary FSA activity related to hypoxia is the Conservation Reserve Program (CRP). CRP is a voluntary program available to agricultural producers to help them safeguard environmentally sensitive land. Producers enrolled in CRP plant long-term, resource-conserving covers to improve the quality of water, control soil erosion, and enhance wildlife habitat. In return, FSA provides participants with rental payments and cost-share assistance. Contract duration is between 10 and 15 years. CRP protects millions of acres of American topsoil from erosion and is designed to safeguard the Nation’s natural resources. By reducing water runoff and sedimentation, CRP protects groundwater and helps improve the condition of freshwater and marine resources. Acreage enrolled in the CRP is planted with resource-conserving vegetative covers, making the program a major contributor to increased wildlife populations in many parts of the country. http://www.fsa.usda.gov/FSA/webapp?area=home&subject=cop r&topic=crp


# I.A.4. U.S. Forest Service

The USDA Forest Service (http://www.fs.fed.us/research/) has a mission to sustain the health, diversity, and productivity of the Nation’s forests and grasslands to meet the needs of present and future generations. The research and development arm of the Forest Service works at the forefront of science to improve the health and use of our Nation’s forests and grasslands. Today, more than 500 Forest Service researchers work across the 50 states, U.S. territories, and commonwealths in a range of biological,

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physical, and social science fields to promote sustainable management of the Nation’ s diverse forests and rangelands and the ecosystem services they provide. The work has a steady focus on informing policy and land management decisions, including those on degraded river ecosystems. The researchers work independently and with a range of partners, including other agencies, academia, nonprofit groups, and industry. The information and technology produced through basic and applied science programs is available to the public for its benefit and use. With over two-thirds of the Nation’s freshwater originating on forested lands, the role of the Forest Service to manage and restore watersheds is indispensable. The Forest Service works to improve management practices and techniques, develop best management practices that are cost-ef fective, and lead efforts in the advancement of markets and payments for ecosystem services. In addition, the Forest Service is working to develop methods for assessing changes in watershed condition as a result of changing demographics and development pressures. The Forest Service has developed a national strategy for consistent characterization of watershed condition. The characterization will be used to prioritize, identify and implement integrated watershed-scale improvement projects on National Forests. Phase 1 will determine the initial (baseline) watershed condition on National Forest System lands using consistent, broadly available data. Phase 2 is a six-step process for classifying watersheds at a finer scale and prioritizing them for treatment, identifying integrated projects that would move them into improved condition classes. Phase 3 involves validating and monitoring watershed condition.


# I.A.5. Economic Research Service

The Economic Research Service (ERS) is the main source of economic information and research from USDA. It brings the perspective of economic analysis to critical issues confronting farmers, agribusiness, consumers, and policy makers. In general, ERS’s data program collects information that can be used in an assessment of policy options in the Mississippi Basin. In conjunction with USDA National Agricultural Statistics Service, ERS collects data through the Agricultural Resource Management Survey on production practices, conservation practices and input use for major crops in major crop producing states. ERS uses these and other data to routinely report on the status and trends of water resource indicators such as tillage practices, conservation practices, chemical input use, and conservation program expenditures for water quality and water conservation. http://www .ers.usda.gov/



# I.B. Department of Commerce I.B.1. National Oceanic and Atmospheric Administration

I.B.1.a. National Ocean Service
National Centers for Coastal Ocean Science, Center for Sponsored Coastal Ocean Research Gulf of Mexico Ecosystems and Hypoxia Assessment Program (NGOMEX). To address the issue of hypoxia in the northern Gulf of Mexico, NOAA’s Center for Sponsored Coastal Ocean Research supports multi-year, competitive, interdisciplinary research projects to develop a fundamental understanding of the northern Gulf of Mexico ecosystem with a focus on the causes and ef fects of the hypoxic zone and the prediction of its future extent and impacts. The research program, which began in 2003 as a result of the HABHRCA 1998 legislation, focuses on developing a predictive capability for the Louisiana continental shelf ecosystem within an adaptive management framework by connecting monitoring, data analysis, model predictions, and management actions with continuous feedback for improvement. Current studies

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are documenting the dynamics of the hypoxic zone over the Louisiana continental shelf and helping to better define the biological, chemical, and physical processes that influence hypoxic zone development and determine its extent, and impacts on fisheries. http://www.cop.noaa.gov/stressors/pollution/current/ gomex-factsheet.html Coastal Hypoxia Research Program (CHRP) . The purpose of the CHRP program, which awarded its first research grants in 2005, is to expand NOAA’s research capability to address hypoxia in other regions experiencing hypoxia beyond the northern Gulf of Mexico. This program is providing research results and modeling tools to help coastal resource managers assess alternative management strategies and make informed decisions for preventing or mitigating the impacts of hypoxia on coastal ecosystems. Determining the causes of hypoxia, developing the capability to predict its occurrence in response to varying levels of anthropogenic stress, and evaluating the subsequent ecological, economic, and social impacts are necessary to assess potential management alternatives. Key areas of CHRP research include: 1) the development of a predictive capability for the spatial and temporal extent of hypoxia given current and potential anthropogenic and natural forcing scenarios and potential management alternatives, 2) t he determination of the current ecological and economic impacts of hypoxia in a region, 3) the development of ecological forecasts and economic valuations of the impacts of changes in a hypoxic region’s spatial or temporal extent, and 3) the development of models that predict the susceptibility of coastal systems to the formation of hypoxic waters, thereby allowing managers to better focus monitoring and assessment programs which should result in more efficient and successful protection and restoration efforts. http://www.cop.noaa.gov/stressors/pollution/current/gomex-factsheet. html National Centers for Coastal Ocean Science, Center for Coastal Monitoring and Assessment NOAA’s Center for Coastal Monitoring and Assessment recently completed an ambitious update to the National Estuarine Eutrophication Assessment (http://ccma.nos.noaa.gov/publications/eutroupdate), which assesses nutrient conditions and direct/indirect impacts in U.S. estuaries. The report thoroughly analyzed available data and assigned condition rankings to estuaries. Geographic accounts of hypoxia are included in the report. Changes in eutrophication status since the last assessment give a sense of the growing incidences of hypoxia in coastal waters.
Office of Ocean and Coastal Resource Management National Estuarine Research Reserve System (NERRS). The principal mission of the NERRS System-Wide Monitoring Program (SWMP) is to develop quantitative measurements of short-term variability and long-term changes in the water quality, biotic diversity, and land-use/land -cover characteristics of estuaries and estuarine ecosystems for the purposes of contributing to ef fective coastal zone management. Long-term monitoring and iterative habitat assessments conducted within the network of 27 NERRS sites are intended to improve the fundamental understanding of the temporal and spatial dynamics of estuarine processes, and to provide baseline information to evaluate subsequent changes in the ecological status of estuarine ecosystems in response to natural perturbations and anthropogenic disturbance. Federal coordination of the NERRS SWMP allows for the recognition of significant ecological trends (improvement or degradation) in estuaries at national, regional, and local levels. Moreover, continued operation of the long-term monitoring effort within the network of representative NERRS sites will provide valuable data to inform assessments and/or models on the cumulative ef fects of environmental stressors in estuarine ecosystems located throughout the U.S. coastal zone. Monitoring at each SWMP site includes continuous measurements of dissolved oxygen levels and monthly nutrient

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sampling. Between 2005 and 2007, NERRS organized the SWMP data into several regional reports, including for the Northeast, Mid-Atlantic and the Pacific coast.


## I.B.1.b. Oceanic and Atmospheric Research Great Lakes Environmental Research Laboratory NOAA’s Great Lakes Environmental Research Laboratory, in collaboration with researchers from the United States and Canada, is undertaking one of the largest, most comprehensive Lake Erie research field programs ever. The International Field Years on Lake Erie (IFYLE) program, which started in 2005 and is projected to continue through 2010, aims to improve understanding of lar ge-scale (time and space) driving forces of ecosystem dynamics. The project has a special research focus on hypoxia and harmful algal blooms and their impacts in Lake Erie. National Sea Grant College Program The National Sea Grant College Program is a competitive awarded research program which leverages state participation with Federal funds. It has focused some research funding on hypoxia research, specifically in the Southeast coastal states.



## I.B.1.c. National Marine Fisheries Service NOAA National Marine Fisheries Service (NMFS) conducts fish surveys/mapping of the entire Gulf coast, with bottom dissolved oxygen as one of the measured parameters, in late June/early July and then again in early September as part of its routine Southeast Area Monitoring and Assessment Program’s (SEAMAP’s) groundfish surveys. The dissolved oxygen data are provided to the public via the Gulf of Mexico Hypoxia Watch website (http://ecowatch.ncddc.noaa.gov/hypoxia), which is managed by NOAA’s National Environmental Satellite, Data, and Information Service . NOAA NMFS scientists have also studied the impact of hypoxia on Gulf coast fisheries. NMFS has also added dissolved oxygen monitoring to its salmonid surveys along the Pacific Northwest to monitor hypoxia on the continental shelf.



# I.C. Department of Defense I.C.1. U.S. Army Corps of Engineers
System-Wide Water Resources Program (SWWRP) . The U.S. Army Corps of Engineers (USACE) is developing watershed, regional, and system-wide assessment tools that are applicable to nutrient and sediment assessments and decision support. As part of the SWWRP, one-, two, and three-dimensional hydrologic, hydrodynamic, and water quality models have been developed and demonstrated in various watershed, riverine, reservoir, and estuarine applications. Example applications include assessment of nutrient and sediment loading in the upper Eau Galle, Wisconsin, watershed and hydrodynamic assessments in the Upper Auglaize, Ohio, watershed. Basic research in nitrogen dynamics has been done in conjunction with pool fluctuations in the Upper Mississippi River

. Response in reservoir water quality to loadings has been studied in numerous USACE reservoirs. Response to nutrient and sediment loads in estuaries has been assessed in the Chesapeake Bay. Data management and visualization toolsusing CorpsGlobe (developed with Google Earth technologies) have been fielded for displaying multidimensional modeling results in a geospatial and temporal context for decision support.

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Chesapeake Bay Environmental Model Package (CBEMP). The CBEMP is the product of a twentyyear partnership among the USACE Baltimore District, the USACE Engineer Research and Development Center, and the EPA Chesapeake Bay Program. The CBEMP is a coupled system of models aimed and managing and reducing eutrophication in the Bay. In particular, the model is aimed at restoring living resources, reducing anoxia, and improving water clarity. The present phase of the study continues the partnership and is focused on the simulation of suspended solids and living resources interactions. The current focus is to assess how management of suspended solids in the watershed may improve Chesapeake Bay water quality and remove water quality impairments, such as low dissolved oxygen, algal blooms, and poor water clarity. USACE has completed an initial calibration with runs of various management scenarios and is revising the model based on the results of the initial scenarios.



# I.D. U.S. Environmental Protection Agency I.D.1. Regional Offices
Great Lakes National Program Office

The EPA Great Lakes National Program Office ( GLNPO) brings together Federal, state, tribal, local, and industry partners in an integrated, ecosystem approach to protect, maintain, and restore the chemical, biological, and physical integrity of the Great Lakes. In respect to hypoxia, the program monitors Lake ecosystem indicators, such as dissolved oxygen, and manages and provides public access to Great Lakes data (http://www.epa.gov/glnpo/monitor.html). Each year, GLNPO uses its funding to assist Great Lakes partners in these areas through grants, interagency agreements, and contracts (http://www .epa.gov/glnpo/ fund/glf.html). In 2002, the GLNPO held a funding competition focused specifically on hypoxia in western Lake Erie. Gulf of Mexico Program Office The Gulf of Mexico Program was formed in 1988 by the EPA as a nonregulatory, inclusive partnership to provide a broad geographic focus on the major environmental issues in the Gulf. The mission of the Program is “to facilitate collaborative actions to protect, maintain, and restore the health and productivity of the Gulf of Mexico in ways consistent with the economic well-being of the Region.” The partnership includes representatives from state and local governments and citizens in each of the five Gulf States; the private sector (business and industry); Federal agencies responsible for research, monitoring, environmental protection, and natural resource management; and the academic community

. This partnership has been very effective in providing a mechanism for addressing complex problems that cross Federal, state, and international jurisdictional lines; a better coordination among Federal, state, and local programs that has increased the effectiveness and efficiency of the long-term commitment to manage and protect Gulf resources; a regional perspective to access and provide the information and address research needs required for effective management decisions; and a forum for affected groups using the Gulf, public and private educational institutions, and the general public to participate in the solution process. Through its partnerships, the Program is working with the scientific community; policymakers at the Federal, state, and local levels; and the public to help preserve and protect the Gulf. It has made significant progress in identifying the environmental issues in the Gulf ecosystem and in or ganizing a program to address those issues. The Program provides a tool to leverage the resources of 18 different Federal agencies, a variety of environmentally minded agencies from the states of Alabama, Florida, Louisiana, Mississippi, and Texas; and numerous public and private organizations. Spanning the broad

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range of environmental concerns from the local to the regional and national presents a significant challenge to the agencies and organizations that have partnered under the banner of the Gulf of Mexico Program, and the Program has met that challenge. Chesapeake Bay Program Office The Chesapeake Bay Program Office represents the Federal government in the implementation of strategies to meet the restoration goals of the Chesapeake Bay Program. The Chesapeake Bay Program is a unique regional partnership leading and directing restoration of the Chesapeake Bay . Its mission is to lead and empower others to protect and restore the Chesapeake Bay ecosystem for future generations. 

# I.D.2. Office of Water
Office of Wetlands, Oceans, and Watersheds The office of Wetlands, Oceans, and Watersheds (OWOW) provides leadership, policy directions, and financial support to ten regional offices as well as states, tribes, and territories. OWOW administers a number of programs that are directed at reducing nutrient pollution and its resultant impairments in watershed and coastal waters. OWOW facilitates states and tribes in the listing of impaired waters, which includes those that have been degraded by excess nutrients and are unable to meet the water quality standards set by states, territories, or authorized tribes. From this list, OWOW provides assistance for the development of total maximum daily loads (TMDLs), each of which is a calculation of the maximum amount of pollution that waters can receive and still meet water quality standards. Using TMDLs, OWOW is investigating the use of water quality trading programs as a possible way to control nutrient pollution. Additional relevant OWOW programs include the nonpoint source program, targeted watershed grants, the National Water Quality Inventory, and National Aquatic Resource Surveys. OWOW also supports and provides leadership to the Mississippi Watershed/Gulf of Mexico Nutrient Task Force Coordinating Committee and participates and provides leadership to the Gulf of Mexico Alliance Water Quality and Nutrient Reduction teams. In addition, OWOW manages the Ocean Survey Vessel Bold , which supports coastal ocean assessment, monitoring and process-oriented research in U.S. waters, including numerous systems impacted by nutrients and hypoxia. EPA’s National Estuary Program (NEP) was established by Congress in 1987 to improve the quality of estuaries of national importance. For information on the NEP, see National and Interagency Efforts, Section I.F.3. Office of Science and Technology The Office of Science and Technology (OST) produces regulations, guidelines, methods, standards, science-based criteria, and studies that are critical components of national programs that protect aquatic environments from the impacts of nutrient pollution. OST provides both technical and financial assistance to states to help them adopt numeric nutrient water quality standards. OST provides direct assistance to states by providing implementation guidance, addressing technical and policy issues that states raise, and providing technical information to support states rulemaking for standards. OST assists in building the scientific capacity for states through support for sampling and monitoring, training, data and statistical analysis, and modeling assistance for developing scientifically defensible nutrient criteria. OST also communicates the importance of managing nutrient pollution by integrating nutrient messages in communications and outreach products related to water quality standards.

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# I.D.3. Office of Research and Development The mission of the Office of Research and Development is to conduct cutting-edge research and foster the sound use of science and technology to fulfill EPA’s statutory and regulatory requirements. Its research laboratories are structured along the risk paradigm by focusing research on exposure, ef fects, risk assessment and risk management. The National Exposure Research Laboratory The National Exposure Research Laboratory (NERL) conducts research and development that leads to improved methods, measurements, and models to assess and predict exposures of humans and ecosystems to harmful pollutants and other conditions in air, water, soil, and food. NERL is conducting atmospheric deposition research and modeling of nitrogen, sulfur, and mercury as stressor inputs to water quality and ecosystem models to provide forecasts of changes in deposition due to management actions stemming from Clean Air Act regulations The National Health and Environment Effects Research Laboratory The National Health and Environment Effects Research Laboratory (NHEERL) conducts research to determine the impacts of environmental stressors on human and ecosystem health as well as the degree to which those stressors cause harm. NHEERL conducts research that improves the science supporting nutrient criteria development in estuaries and coastal waters. Case studies in Pensacola Bay , Florida, and Yaquina Bay, Oregon, have provided approaches that may be used to develop nutrient criteria and protect aquatic life. Additionally, NHEERL research on Gulf hypoxia is developing statistical, semi-empirical and coupled numerical model applications, data products, and other tools to quantify the biogeochemical processes and ecosystem responses to nutrient loads, quantify sources of uncertainty in nutrient load–hypoxia response relationships, and forecast the effects of nutrient management actions and load reduction targets on the extent of hypoxia. In situ observations and analyses from coastal and continental shelf cruises provide the empirical bases for the numerical simulation models of eutrophication and hypoxia on the continental shelf. Through an interagency agreement with the Naval Research Laboratory (NRL), a coupled water quality-sediment diagenesis model is being integrated into the NRL coastal ocean hydrodynamic model. The integrated numerical simulation model will provide improved mechanistic understanding of the processes regulating Gulf hypoxia.

The National Center for Environment Assessment

The National Center for Environment Assessment develops new methods for r isk assessment and applies those methods to the cutting-edge issues that the U.S. EP A

faces.
The National Risk Management Research Laboratory The National Risk Management Research Laboratory (NRMRL) conducts research and technical assistance to provide the scientific basis to support the development of strategies and technologies to protect and restore ground water, surface water, and ecosystems impacted by man-made and natural processes. NRMRL conducts nutrient fate and transport modeling, incorporating surface water-groundwater interactions, in the Mississippi River Basin. It also conducts research to improve manure management practices and reduce impacts of manure runoff on water quality. In addition, the Laboratory’s research on best management practices focuses on evaluating how restoration techniques may enhance nutrient retention and attenuation in watersheds that are small enough for management practices to have a significant impact.

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The National Center for Environmental Research The National Center for Environmental Research (NCER) supports high-quality research that will provide the scientific bases for national environmental decisions. NCER supports cutting-edge, extramural research through competitions for grants, fellowships, and innovative small business research contracts. NCER’s Science to Achieve Results (STAR) Program funds research grants and graduate fellowships related to various aspects of hypoxia, including nutrient source, fate, and transport. Overall, STAR awards about 150 research grants and 125 graduate fellowships each year. NCER also makes awards under joint Request for Applications with partnering agencies. These grants and fellowships have been awarded to universities and nonprofit research institutions in all 50 states, Guam, Puerto Rico, and the District of Columbia.
Environmental Monitoring and Assessment Program The Environmental Monitoring and Assessment Program (EMAP) is a research program to develop the tools necessary to monitor and assess the status and trends of national ecological resources. EMAP’ s goal is to develop the scientific understanding for translating environmental monitoring data from multiple spatial and temporal scales into assessments of current ecological condition and forecasts of future risks to natural resources. The EMAP program also provides extramural funding through the STAR program. Through their Great Rivers Project, EMAP is providing an assessment of the ecological conditions in the Mississippi River Basin using a probability-based sampling design. This assessment will allow for statelevel assessment of those rivers within or adjacent to a state’s borders. National Environmental Scientific Computing Center and the Environmental Modeling and Visualization Laboratory The National Environmental Scientific Computing Center and the Environmental Modeling and Visualization Laboratory (EMVL) is an EPA-managed, contractor-operated facility designed to support EPA’s scientific modeling and large-scale data management research programs through the application of high performance computing and visualization tools. The mission of EMVL falls in two categories: 1) to provide the high performance computing resources necessary to support environmental research of global proportions and improved science for the development of regulations and 2) to assist researchers in translating data into knowledge by using visual images to facilitate insight into that data. Currently
, EMVL is supporting NHEERL research on Gulf hypoxia by assisting in the development of an integrated hydrodynamic, water quality, and sediment diagenesis simulation model for hypoxia in the Gulf of Mexico.



# I.E. Department of Interior I.E.1. U.S. Geological Survey

Integrated Studies of Coastal Ecosystems USGS conducts long-term assessments of the environmental and ecological conditions in aquatic ecosystems across the Nation in selected bays, estuaries, and other coastal environments. The research addresses a broad range of science issues related to environmental health and ecosystem management, including nutrient dynamics, algal blooms, and the role of terrestrial contributions of contaminants. The research in these ecosystems is coordinated closely with other Federal and local governmental agencies. Information about such programs are available at:

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San Francisco Bay: http://sfbay.wr.usgs.gov/access/wqdata/ Chesapeake Bay: http://chesapeake.usgs.gov/ Gulf of Mexico: http://toxics.usgs.gov/hypoxia/ Tampa Bay: http://gulfsci.usgs.gov/tampabay/ Galveston Bay: http://gulfsci.usgs.gov/galveston/ Boston Harbor: http://woodshole.er.usgs.gov/project-pages/bostonharbor/ Puget Sound: http://puget.usgs.gov/

National Streamflow Information Program The National Streamflow Information Program (http://water.usgs.gov/nsip) provides streamflow information at more than 7,000 stream gauges nationwide, funded by the USGS and over 800 local, state, and other Federal agencies, many of which represent downstream measuring sites for rivers dischar ging to the coast. These data are essential to understanding the role of and trends in freshwater contributions to coastal ecosystems and to quantifying the associated loadings of nutrients and other chemicals. Water Quality Monitoring The USGS National Stream Quality Accounting Network measures the annual transport of nutrients, dissolved solids, selected pesticides, and suspended-sediment from selected lar ge rivers to coastal waters of the United States and from major inland sub-basins for priority lar ge rivers, where the source origins of chemical loads are needed to guide management actions. The network includes monitoring at approximately 18 major coastal rivers that contribute over 80 percent of the total dischar ge of streamflow
, nitrogen, phosphorus, and suspended sediment to coastal waters of the United States. Nineteen additional stations within the Mississippi River Basin are monitored to define the source origins of streamflow and nutrients ultimately delivered to the Gulf of Mexico to guide management actions to mitigate hypoxia in the northern Gulf of Mexico. Other major river systems where chemical loads are monitored at inland stream sites to guide management actions include the Chesapeake Bay and San Francisco Bay watersheds. USGS water quality monitoring of flow and chemical delivery to coastal waters is used to make estimates of national coastal delivery, such as those developed by the Heinz Center (http://www.heinzctr.org/ecosystems/2002report/national/mvmt_n.shtml). The National Water Quality Assessment (NAWQA) (http://water.usgs.gov/nawqa/) Program conducts assessments of the quality of the Nation’s waters to provide an understanding of water quality conditions and how those conditions may vary locally, regionally, and nationally; whether conditions are getting better or worse over time; and how natural features and human activities af fect those conditions. NA WQA Program scientists collect and interpret data about surface- and groundwater chemistry , hydrology , land use, stream habitat, and aquatic life in parts or all of nearly all 50 states using a nationally consistent study design and uniform methods of sampling analysis. Water quality monitoring for national status and trends provide important data on the occurrence and distribution of nutrients and other water quality constituents in representative basins throughout the Nation. These data are used to develop the SPARROW and other water quality models that provide significant knowledge of the source areas of nutrients and the human activities that affect nutrient loads. Groundwater Resources Program Freshwater delivered through groundwater discharge to coastal waters is essential to ecosystem function and coastal circulation, and can have a significant influence on the health of these ecosystems.

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The USGS Groundwater Resources Program (http://water.usgs.gov/ogw/gwrp) conducts studies along the Atlantic coast related to ground-water discharge and its role in coastal salinity balance and the role of the associated nutrient contributions in coastal hypoxia. Additional studies in these areas are conducted on a cooperative basis with local government agencies. Nutrient Dynamics and Biochemical Cycling in Wetland and Riverine Ecosystems USGS conducts research to improve the understanding of the biogeochemical cycling of nutrients in riverine and wetland ecosystems. Ecosystem management of ecosystems is emerging as an effective means of protecting natural resources and reducing nutrient inputs to receiving waters. Ef fective ecosystem management requires knowledge of the biogeochemical cycling of nutrients (requiring analysis of internal and external sources and sinks of nutrients) and understanding of the processes that control these sources and sinks. In numerous aquatic ecosystems, many of these processes are poorly understood as are their potential interactions with other ecosystem properties, such as plant production. This research is being conducted primarily by the USGS National Research Program ( http://water.usgs.gov/nrp) , the NAWQA Program, the National Wetlands Research Center ( http://www.nwrc.usgs.gov) , and the Upper Midwest Environmental Science Center ( http://www.umesc.usgs.gov)

.


# I.F. National and Regional Interagency Efforts I.F.1. Integrated Ocean Observing Systems The U.S. Integrated Ocean Observing System (IOOS) (http://www.ocean.us) is a coordinated national network of observations and data transmission, data management, and communications intended to routinely and continuously acquire and disseminate quality controlled data and information on current and future states of the oceans and Great Lakes from the global scale of ocean basins to local scales of coastal ecosystems. The IOOS is part of the U.S. Integrated Earth Observing System, the U.S. contribution to the Global Ocean Observing System (http://www.ioc-goos.org), and a contribution to the international Global Earth Observation System of Systems.



# I.F.2. National Monitoring Network and the National Water Quality Monitoring Council In response for calls for a National Water Quality Monitoring Network for streamflow and the associated chemical delivery to coastal waters, the National Water Quality Monitoring Council (http:// acwi.gov/monitoring), a subgroup of the Advisory Committee on Water Information, was charged with designing this network. The design was developed by 80 representatives working through the National Water Quality Monitoring Council, including representatives from Federal, state, and local government organizations, universities, water associations, and the private sector. The Council is co-chaired by the USGS and the EPA, and its other Federal members include NOAA, Tennessee Valley Authority, USACE, USDA, and the remaining Department of the Interior (DOI) agencies. The Network will coordinate water quality monitoring across the Nation to provide a comprehensive database that supports assessment of the health of ocean, coastal, and Great Lakes resources. Additional information on the national monitoring network, including reports for the pilot phase conducted in three geographic areas (Delaware Bay , San Francisco Bay, and Lake Michigan) is available on at http://acwi.gov/monitoring/network.

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# I.F.3. National Estuary Program EPA’s National Estuary Program (NEP; http://www.epa.gov/owow/estuaries) is a national network of 28 programs working for collaborative solutions for estuaries designated by Congress as of critical importance. Created in 1987 under the Clean Water Act, the NEPs are charged with protecting and restoring U.S. estuaries by engaging state and Federal agencies, nongovernmental or ganizations, and local communities in planning and management. EPA provides base funding and Federal oversight for each of the NEPs, but program management is typically the responsibility of an estuary stakeholder -based management committee. Section 320 of the Clean Water Act (National Estuary Program) states that one of the main purposes of the NEP is to develop a comprehensive watershed ecosystem plan for the conservation and management of natural resources in NEP estuaries. NEPs are required to have inclusive stakeholder representation on any management or advisory committee. Representatives of state and Federal agencies (including EP A, USDA, and NOAA), interstate or regional agencies, local governments, industry and business, public and private educational institutions and the general public, should comprise such a committee. 

# I.F.4. Regional Efforts
Chesapeake Bay The Chesapeake Bay Program (http://www.chesapeakebay.net) is a regional partnership charged with leading and directing restoration of the Chesapeake Bay. This partnership includes representatives from Maryland, Virginia, the District of Columbia, Pennsylvania, the Chesapeake Bay Commission, and citizen advisory groups. The Federal government is formally represented by the U.S. EPA, but a number of additional Federal partners are involved, including NOAA, USDA, National Park Service, USGS, USFWS, Federal Housing Administration, U.S. Coast Guard, National Aeronautics and Space Administration (NASA), U.S.Postal Service, General Services Administration, National Capital Planning Commission, Department of Defense, and Department of Education. Scientific guidance is provided by a Scientific Technical Advisory Committee. The Chesapeake Bay Program is leading efforts to improve water quality in the Bay, especially related to nutrients, with the goal of improving dissolved oxygen levels and habitat quality through working with farmers, developers, homeowners and local governments to reduce pollutants. In 1998, the EP A and state members of the Bay Program (known as the Chesapeake Executive Council) signed the second Chesapeake Bay Agreement which called for a 40% reduction in nitrogen and phosphorus inputs by the year 2000. This nutrient reduction target was reaffirmed in 1992 and expanded to include Bay tributaries and a call for detailed tributary nutrient management strategies. A third agreement, Chesapeake 2000
, also reaffirmed the 40% reduction target, but extended the target date to 2010 and outlined more specific strategies to meet the goal.

Northern Gulf of Mexico The Mississippi River/Gulf of Mexico Watershed Nutrient Task Force was formed in 1997 to understand the cause and effects of eutrophication in the Gulf of Mexico and to coordinate and support nutrient management activities within the Mississippi River Watershed. Members of the Task Force include executive level representatives from ten states, the EPA, NOAA, USACE, DOI, and USDA. Task Force members have designated senior managers within their respective agencies to serve on a Coordination Committee, which advises the Task Force on actions and manages workgroups and committees. In 2001, the Task Force released an “Action Plan” (Mississippi River/Gulf of Mexico Watershed Nutrient Task Force 2001) to address Gulf hypoxia and nutrient pollution within the watershed,

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setting a goal of reducing the annual size of the hypoxic zone to 5,000 km 2 

### by 2015. Following a comprehensive scientific reassessment process that included an EP A

Science Advisory Board Report (U.S. EPA 2007), the Task Force released a “2008 Action Plan” (Mississippi River/Gulf of Mexico Watershed Nutrient Task Force 2008) that reaffirmed the goal of 5,000 km 2 

### , although it was recognized the 2015 target may not be met, and placed additional focus on the management of phosphorus in addition to nitrogen. To reach this goal, the “2008 Action Plan” (Mississippi River/Gulf of Mexico Watershed Nutrient Task Force 2008) also recommended nitrogen and phosphorus reductions of at least 45%. The Gulf of Mexico Alliance was formed in 2004 as a partnership between the five Gulf States (Florida, Alabama, Mississippi, Louisiana, and Texas) with the goal of increasing regional collaboration to protect and enhance the ecological and economic health of the Gulf. Although NOAA and EPA are designated as Federal coordinators, the Alliance actively partners with NSF, NASA, USACE, USDA, DOD, DOE, DOI, Department of Transportation, Department of Health and Human Services, and the Department of State. Following its formation, the Alliance released a three-year action plan in 2006 (http://www.gulfofmexicoalliance.org) that identified reducing nutrient inputs as one of five priority issue areas. Under the priority, increasing regional coordination for the development of TMDL’s and addressing the hypoxic zone in the northern Gulf through an aligned Gulf States position were identified as key actions. Through its second action plan that began in 2009, the Alliance will continue to facilitate the development of coordinated nutrient criteria and reduce hypoxia in coastal waters and estuaries through increased coordination with Gulf Hypoxia Task Force and targeted watershed strategies.
Narragansett Bay Significant cooperative efforts have been undertaken in Narragansett Bay to both understand and mitigate hypoxia. The Narragansett Bay Estuary Program (NBEP, http://www.nbep.org) was established in 1993 as part of the NEP . EPA, USDA, Rhode Island and Massachusetts state environmental agencies, academic partners (University of Rhode Island) and other nongovernmental stakeholders participate in the management of NBEP. The NBEP also works closely with other Federal partners including NOAA, USACE, USGS, and USFWS. Since 1998, scientists from the NBEP, along with volunteer scientists from academic institutions and state agencies, have been conducting monthly (or more often) dissolved oxygen surveys. The data from these surveys are available at: http://www.geo.brown.edu/georesearch/ insomniacs/links.html. A major menhaden fish kill in August 2003 was investigated by NBEP scientists in the context of the developing knowledge of dissolved oxygen dynamics in Narragansett Bay

. The Rhode Island state legislature passed legislation the following year to reduce nitrogen inputs from wastewater treatment plants.

Great Lakes In December 2004, the Great Lakes Regional Collaboration was launched as a result of a presidential executive order, creating a unique partnership of key members from Federal, state, and local governments, tribes, and other stakeholders for the purpose of developing a strategic action plan. The Federal members of the Task force are cabinet level secretaries of the Departments of State, Commerce, Defense, Agriculture, Housing and Urban Development, Transportation, and the Interior; EPA; and the Council on Environmental Quality. This strategic plan, the Great Lakes Regional Collaboration Strategy , was signed in December 2005 and is intended to build upon the extensive regional ef forts to date, working together toward a common goal of restoring and protecting the Great Lakes ecosystem for this and future generations. Although hypoxia is not particularly emphasized, nutrient reduction is an important part of the strategy. http://www.glrc.us

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Puget Sound A dramatic 2003 fish kill in Hood Canal which was caused by the temporary upwelling of hypoxic waters precipitated strong public and scientific interest in understanding hypoxia dynamics in the Puget Sound ecosystem. The goal of the Hood Canal Dissolved Oxygen Program (HCDOP) supported through Congressional funding to the University of Washington is to determine the sources of low dissolved oxygen in Hood Canal and its effect on marine life. HCDOP has worked with local, state, Federal, and tribal government policy makers to develop potential corrective actions that will restore and maintain a level of dissolved oxygen that will not stress marine life. HCDOP is a partnership of 28 or ganizations that conducts monitoring and analysis and develops potential corrective actions to address the low dissolved oxygen problem in Hood Canal, including Federal (NOAA, USGS, USFWS. USACE, EP A

and U.S. Navy), state, and tribal entities. http://www.hoodcanal.washington.edu In 2007, the state legislature created the Puget Sound Partnership which took over the role of the Puget Sound Action Team. The goals of the partnership are to prioritize cleanup and improvement projects; coordinate Federal, state, local, tribal, and private resources; encourage cooperation; and make decisions based on sound science—some of which will be provided by the HCDOP . Several Federal agencies are represented on the partnership councils, including NOAA, Navy, USGS, USFWS, and EPA. http://www.
psp.wa.gov Long Island Sound The Long Island Sound Study (LISS) was formed by EPA, the State of New York, and the State of Connecticut to cooperatively address the degraded ecosystem within Long Island Sound. This partnership also involves relevant nonprofit organizations, concerned citizens, as well as NOAA, EPA, USGS, USFWS, and USDA. The LISS completed a Comprehensive Conservation and Management Plan that identified hypoxia in the Sound as one of seven priority issues. T o address hypoxia, in 1998 the LISS adopted a 58.5% nutrient reduction target for nitrogen by 2014, and nutrient management plans for New York and Connecticut were formally approved by EPA in 2001. These nutrient management plans are focusing on both nonpoint and point source pollution. Scientific support for the management plan is developed through the LISS Scientific Technical Advisory Committee, with financial support provided through a cooperative agreement among the EPA and the New York and Connecticut Sea Grant Programs. The LISS also funds the monitoring of hypoxia, water quality, and plankton. http://www. longislandsoundstudy.net

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# Appendix II. Geographic Case Studies
Introduction and Background to Case Studies
The case studies presented here highlight selected coastal ecosystems that are geographically diverse and represent the spectrum of estuarine and coastal ocean ecosystems af fected by hypoxia. The selected cases describe the range of circumstances, understanding, and scientific and management approaches across the United States (Figure A1). They provide examples of monitoring and research programs that have been or are being used to develop management plans. In some instances the outcomes ofimplemented management measures are also presented and should serve as encouragement that nutrientrelated dissolved oxygen problems can be improved with proper management. The exception is hypoxia along the Oregon coast, which has occurred since 2002 and is more related to climate impacts on upwelling-favorable winds on the west coast. Yet, coastal management is still pertinent to the Oregon shelf which has thriving coastal fisheries negatively impacted by hypoxia. These case studies provide the basis for successful management approaches that may be used in other systems in the United States.

Figure A1. Geographic locations of hypoxia case studies. Large blue dots represent cases studies; red dots represent systems that have a documented hypoxia issue.

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 84 

## AppendicesSiteWaterbodyarea (km2)Watershedarea (km2)Averagedepth(m)Tidalheight(m)Freshwaterinflowm3/dAveragesalinity(psu)WatershedpopulationNEEA Results*:Dissolvedoxygen ratingEarly1990sEarly2000sLong Island Sound3,25912,773201.91.55 x 107284.91 x 106ModerateHighLake Erie25,744---190---0---------Chesapeake BayMainstem6,974795847.30.451.05 x 108166.41 x 106HighHighPensacola Bay477176503.00.422.58 x 107183.71 x 105ModerateLowNorthern Gulf ofMexico31,7432,968,304200.291.53 x 109297.30 x 107HighHighYaquina Bay146342.11.96.60 x 105226.03x103LowLowOregon Shelf26,600------3 - 4(nearshore)5.6 x 10832---------Hood Canal3962768702.27.45 x 106263.49 x 104ModerateHigh* from Bricker, S., B. Longstaff, W. Dennison, A. Jones, K. Boicourt, C. Wicks and J. Woerner. 2007. Effects of Nutrient Enrichment in the Nation’sEstuaries: A Decade of Change, National Estuarine Eutrophication Assessment Update. NOAA Coastal Ocean Program Decision Analysis Series No. 26.National Centers for Coastal Ocean Science, Silver Spring, MD. 322 pp. http://ccma.nos.noaa.gov/news/feature/Eutroupdate.htmlTable A1. Comparison of Physical Systems Represented by Case Studies in Appendix II

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# Long Island Sound
Physical Description of the System
Long Island Sound is a large (3,056 km 2 

### ) glacial outwash estuary, shared by the States of Connecticut and New York (Figure 1). Its unique configuration connects it with the Atlantic Ocean via the Race in the east and via the East River in the west. The Connecticut River, very near the Sound’s eastern terminus, contributes about two thirds of its freshwater input. The Housatonic and Thames Rivers also contribute significant volumes of fresh water to this system. The influence of the East River (a tidal strait) promotes a stratified salinity structure in the western sound especially in the spring runoff period when freshwater from the Hudson River basin is transported into western Long Island Sound. Salinity variability is less distinct in the eastern sound where salinities tend to be higher

. Tidal amplitude ranges from about two meters in the west to less than one meter in the east. The Sound is moderately flushed, with mean residence times of two to three months. A highly developed watershed contributes to low dissolved oxygen problems (from Bricker et al. 1997, 2007). 

# History of Hypoxia (issue, causes, economic, and ecosystem impacts)
Long Island Sound has a large and highly developed watershed (Table A1). Nitrogen contributions from the watershed, combined with strong summer thermal stratification in its western half, renders Long Island Sound susceptible to seasonal hypoxia. Since 1985, the causes and ef fects of hypoxia have been the subject of intensive monitoring, modeling, and research through the Long Island Sound Study (LISS), part of the EPA National Estuary Program (see Appendix I, Interagency Efforts). Hypoxia most seriously affects the strongly stratified western half of the Sound where dissolved oxygen concentrations fall well below Connecticut and New York’s water quality standards each summer (Figure 2). Dissolved oxygen levels below 3 mg/L are usually observed, levels below 2 mg/L are not uncommon, and during some years portions of the Sound’s bottom waters become anoxic (<1 mg/L; Figures 2 and 3). Long Island Sound is surrounded by a highly urbanized landscape, including New York City in the west and large, sprawling populated areas in Long Island and Connecticut. Primary sources of nitrogen include Figure 1. Location of Long Island Sound (P. Stacey, CT DEP).

Figure 2. The frequency and location of hypoxia in Long Island Sound bottom waters from 1991-2008 (P. Stacey CT DEP).

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sewage treatment plants, nonpoint source runoff, and atmospheric deposition, all driven by human influences in the watershed and airshed (Figure 4). Monitoring of Long Island Sound conducted by the Connecticut Department of Environmental Protection on behalf of the LISS has shown an annual recurrence and persistence of hypoxia over the last 15 years. Despite significant reductions in nitrogen loads by both Connecticut and New York under a total maximum daily load (TMDL) approved in 2001, dissolved oxygen improvements have been slow and masked by weather-driven variability and effects of climate change. Considering that nature contributes, at most, about 10,000 metric tons of nitrogen every year to the Sound from weathering and nitrogen fixation in the watershed, the nearly 38,000 metric tons per year added by more than 100 sewage treatment plants located along the coast and throughout the drainage basin have greatly enriched the ecosystem (Figure 5). Another 12,500 metric tons of nitrogen are contributed each year from nonpoint sources coming from excessive fertilizer added to lawns or agricultural crops, atmospheric emissions (from automobiles, power plants, and industry), and human and animal wastes (including home septic systems). Population continues to grow within the already densely populated Long Island Sound basin. This growth contributes to the nitrogen load through sewage treatment plants as well as through various nonpoint sources, and the impact is large. Since 1985, Connecticut’s land conversion rate to developed uses was 11.3% for a population that had grown about 8.6%. Per capita consumption of land is outstripping population growth in Connecticut and throughout the basin. Nitrogen enrichment, coupled with the Sound’s sensitivity to hypoxia due to relatively long residence time and seasonally strong stratification, leads to unhealthy conditions that are environmentally and economically costly for the Sound and its users. Furthermore, submerged aquatic vegetation (SAV) decline has been observed in many eastern Long Island Sound embayments and is Figure 3. Areal extent and duration of Long Island Sound hypoxia, 1987-2007. The average areal extent is 521 km 2 and average duration is 58 days. (P. Stacey CT DEP).

Figure 4. Sources of nitrogen to Long Island Sound – baseline condition (P.Stacey, CT DEP).

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believed to be linked to nitrogen enrichment. Nitrogen reductions to protect SA V will be much more stringent and difficult to attain than for low dissolved oxygen (Stacey 2007). 

# Science and Management Actions (to date and planned)
Since 1985, the causes and effects of hypoxia have been the subject of intensive monitoring, modeling, and research through the LISS. Through the Long Island Sound partnership, a dissolved oxygen TMDL was completed by Connecticut and New York and approved by U.S. EPA in 2001. Both states have aggressively pursued sewage treatment plant nitrogen control using biological processes, and nitrogen loads are trending downward. New York has relied upon traditional permitting programs to limit individual sewage treatment plants while Connecticut has instituted a statewide nitrogen trading program, called Nitrogen Credit Exchange, for 79 municipal sewage treatment plants (CT DEP 2007). The trading program works as an economic engine, forcing action towards the most cost-ef fective and environmentally beneficial projects. Collectively, the two states have accomplished a 30% reduction in sewage treatment plant nitrogen loads towards the TMDL target of 60–65% by 2014. The nitrogen trading program has proven to be a viable alternative for improving dissolved oxygen conditions in Long Island Sound and has accelerated the schedule to meet the TMDL wasteload allocation deadline. Potential savings with nitrogen trading are in the $200-400 million range over individual permitting approaches. The Nitrogen Credit Exchange could serve as a working model for incentive-based trading in other states. Connecticut and New York are also relying on stormwater permitting and nonpoint source programs to meet a 10% reduction target for urban and agricultural lands. However, the nonpoint sources are more difficult and costly to control, especially atmospheric deposition, much of which originates from jurisdictions outside of Connecticut and New York. If attained, promised reductions from Federal Clean Air Act initiatives will help Long Island Sound tremendously. The LISS is also working with Massachusetts, New Hampshire, and Vermont, states that share the Long Island Sound watershed, to ascertain what level of reduction might be achieved in a cost-ef fective manner by those states. Figure 5. Estimated nitrogen loads to Long Island Sound, 1991-2004 (P . Stacey, CT DEP).

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# Future Outlook
Population continues to grow within the already densely populated Long Island Sound basin. This growth contributes to the nitrogen load from sewage treatment plants as well as nonpoint sources. Despite the high land conversion rate since 1985, nitrogen loads have been reduced by some degree, largely through active management of sewage treatment. However, nutrient-related water quality problems have not improved as much as anticipated, in part, because of the high rate of per capita land consumption (sprawl) relative to population growth. As a result, management measures are offset by the increase in nutrient inputs related to population increases. In addition, the ef fects of climate change appear to have strengthened stratification in the Sound, exacerbating hypoxia. 

# References

Bricker S, Clement C, Frew S, Harmon M, Harris M, Pirhalla D. 1997. NOAA ’s Estuarine Eutrophication Survey. Volume 2: Mid-Atlantic Region. Silver Spring, MD. Office of Ocean Resources Conservation Assessment. 51 pp. Bricker S, Longstaff B, Dennison W, Jones A, Boicourt K, Wicks C, Woerner J. 2007. Effects of Nutrient Enrichment in the Nation’s Estuaries: A Decade of Change, National Estuarine Eutrophication Assessment Update. NOAA Coastal Ocean Program Decision Analysis Series No. 26. National Centers for Coastal Ocean Science, Silver Spring, MD. 322 pp. http://ccma.nos.noaa.gov/news/feature/Eutroupdate.html CT DEP. 2007. Connecticut’s Nitrogen Credit Exchange – An Incentive- based Water Quality Trading Program. The Connecticut Department of Environmental Protection, Bureau of Waer Protection and Land Reuse. Hartford, CT. Stacey PE. Personal Communication. Connecticut Department of Environmental Protection. Stacey PE. 2007. Long Island Sound, Ct & NY: Point source reductions lessened hypoxia in 1990s. In: Bricker S, Longstaff B, Dennison W, Jones A, Boicourt K, Wicks C, Woerner J. 2007. Effects of Nutrient Enrichment in the Nation’s Estuaries: A Decade of Change, National Estuarine Eutrophication Assessment Update. NOAA Coastal Ocean Program Decision Analysis Series No. 26. National Centers for Coastal Ocean Science, Silver Spring, MD. 322 pp. http://ccma.nos.noaa.gov/news/feature/Eutroupdate.html

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# Lake Erie
Physical Description of the System
Lake Erie is the shallowest of the Great Lakes with an average depth of 19 meters. It has three identifiable sub-basins: the eastern, central, and western basins. Lake Erie is deepest in the eastern basin and becomes increasingly shallow towards the west. Because it is the shallowest of the Great Lakes, it warms more quickly in the summer and cools more rapidly in the fall. The shallow depth and warm temperatures lead to high productivity. Thermal stratification, caused by warming of the upper layer, occurs throughout the Lake every summer. However, vulnerability to hypoxia is not consistent basin to basin. Eighty percent of the Lake’s water inflow comes from the Detroit River, but most of the nutrient and sediment loading comes from other tributaries, notably the Maumee River . Lake Erie receives the highest sediment loads of all the Great Lakes (Letterhos 2007). 

# History of Hypoxia (issue, causes, economic and ecosystem impacts)

The central basin of Lake Erie is the most vulnerable to hypoxia because it is sufficiently deep to develop thermal stratification, but the volume of water below the thermocline (or temperature gradient) is small enough that oxygen is depleted quickly. The water below the thermocline is cut off from oxygen in the surface water due to the stratification. The bottom waters in the central basin frequently go hypoxic during late summer through early fall (Charlton 1980, Rosa and Burns 1987). In contrast, the much deeper eastern basin, which also develops thermal stratification, has a much higher water volume below the thermocline, so the oxygen is not depleted before the water column mixes when the cold weather returns in the fall.The shallower western basin experiences stronger winddriven circulation that prevents stratification, and thus hypoxia, during most summers. Although late summer hypoxia is a natural phenomena in Lake Erie probably dating back thousands of years (Delorme 1982), evidence suggests that the summer oxygen depletion rates increased during the 1950s and 1960s due to increased phosphorus loadings from point and nonpoint sources. Even the shallower western basin developed hypoxia during low wind periods (Hartman 1972, Leach and Nepszy 1976, Beeton 1963). Concerted phosphorus reduction programs led to improved conditions until late 1990s. However, the extent of bottom water hypoxia has returned to pre-action levels. The cause is much less clear, but likely related to increases in nutrient inputs from nonpoint sources (Richards, Unpublished Data) (Figure 1). Total phosphorous and soluble reactive phosphorous concentrations in the mainstem of the Lake have remained low, but levels in the nearshore areas are rising. Phosphorus, however, may no longer be the limiting nutrient; nitrogen and carbon may be playing greater roles (Letterhos 2007). The effects of hypoxia on the Lake Erie ecosystem are not entirely clear. Some benthic species, such as burrowing mayflies (an important prey species), disappeared during periods of the most intense hypoxia (i.e., 1950s and 1980s) (Britt 1955, Carr and Hiltunen 1965, Krieger et al.1996). The loss of a thermal refuge due to low oxygen in the cooler bottom waters probably contributed to the decline of Figure 1. Hypoxia in Lake Erie in 2006. Source: IFYLE Program.

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several commercially valuable benthic fishes (e.g., lake whitefish and burbot). The arrival of the zebra mussel in the 1980s caused tremendous ecological change and may be contributing to hypoxia. The effect on Lake Erie fisheries is currently an area of active research (see Appendix I, NOAA/GLERL International Field Years on Lake Erie). 

# Science and Management Actions (to date and planned)
The Great Lakes Water Quality Agreement of 1978 (http://www.epa.gov/glnpo/glwqa/1978/index.html) was a binational agreement between Canada and the United States which set strict limits on phosphorus loadings, a goal which was met in the 1980s (Hawley et al. 2006). The agreement tar geted inputs from wastewater treatment plants, limited the use of phosphorus in deter gents, and led to development of best management practices to reduce phosphorus from agricultural runof f. By the late 1980s, the goals outlined in the agreement were largely achieved and the incidence and extent of hypoxia significantly decreased (Hawley et al. 2006). However, the severity of hypoxia and levels of phosphorus in the lake began to increase in the 1990’s, although the cause is relatively unknown (Hawley et al. 2006). In 1987 the governments of Canada and the United States also made a commitment, as part of the Great Lakes Water Quality Agreement, to develop a Lakewide Management Plan for the Great Lakes. The Lakewide Management Plan (LaMP) for Lake Erie (http://www.epa.gov/greatlakes/erie.html) is coordinated by Federal, state, and provincial government agencies in the two countries. Under the guidance of these agencies, the LaMP unites a network of stakeholders in actions to restore and protect the Lake Erie ecosystem. The LaMP provides an opportunity to link efforts to work toward the common goal of restoring Lake Erie for future generations. In 2005, the International Field Years on Lake Erie (IFYLE) was initiated by researchers from the United States and Canada to conduct comprehensive research in the Lake, with an initial focus on hypoxia and harmful algal blooms. The primary objectives of this effort focus on quantifying the spatial extent of hypoxia to enable the development of forecasts as well as to determine the ecological consequences of hypoxia to Lake Erie living resources. In December 2005, the Great Lakes Regional Collaboration (GLRC) released a Strategy to Restore and Protect the Great Lakes (http://www.glrc.us/strategy.html), which addressed nonpoint source pollution in the recommendations. More recently, the President’s 2010 Budget provided $475 million in EPA’s budget for a new Environmental Protection Agency-led, interagency Great Lakes Restoration Initiative. This Great Lakes Restoration Initiative is guided by the GLRC strategy and will tar get the most significant problems in the region, including invasive aquatic species, nonpoint source pollution, and contaminated sediment. 

# Future Outlook
The IFYLE will hopefully elucidate the most important factors contributing to the increase in hypoxia since the mid-1990s. Further, the impressive international cooperative effort in the region, represented by the Great Lakes Water Quality Agreement, LaMPs, Great Lakes Regional Collaboration (http://www. glrc.us/), and the Great Lakes Restoration Initiative, to identify and reduce stressors in Lake Erie and the other Great Lakes improves the chance of reducing hypoxia in the future. Management actions that reduce nutrient loadings in Lake Erie may have positive ramifications for both freshwater harmful algal blooms and hypoxia.

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# References
Beeton AM. 1963. Limnological survey of Lake Erie in 1959 and 1960. Great Lakes Fishery Commission Technical Report 6: 1-32.

Britt NW. 1955. Stratification in western Lake Erie in summer of 1953: effects on the Hexagenia (Ephemeroptera) population. Ecology 36: 239-244. Carr JF, Hiltunen K. 1965. Changes in the bottom fauna of western Lake Erie from 1930 to 1961. Limnology and Oceanography 10: 551-569. Charlton MN. 1980. Oxygen depletion in Lake Erie: has there been any change? Canadian Journal of Fisheries and Aquatic Sciences 37: 72-81. Delorme LD. 1982. Lake Erie oxygen: the prehistoric record. Canadian Journal of Fisheries and Aquatic Sciences 39: 1021-1029. Hartman WL. 1972. Lake Erie: effects of exploitation, environmental changes and new species on the fishery resources. Journal of the Fisheries Research Board of Canada 29: 899-912. Hawley N, Johengen TH, Rao YR, Ruberg SA, Beletsky D, Ludsin SA, Eadie BJ, Schwab DJ, Croley TE, Brandt SB. 2006. Lake Erie Hypoxia Prompts Canada-U.S. Study. EOS Transactions 87(32): 313-324. Krieger KA, Schloesser DW, Manny BA, Trisler CE, Heady SE, Ciborowski JJH, Muth KM. 1996. Recovery of burrowing mayflies (Ephemeroptera: Ephemeridae: Hexagenia ) in western Lake Erie. Journal of Great Lakes Research 22: 254-263. Leach JH, Nepszy SJ. 1976. The fish community in Lake Erie. Journal of the Fisheries Research Board of Canada 33: 622-638. Letterhos J. 2007. Lake Erie Background from a Nutrient Perspective Prepared for the Ohio Phosphorus T ask Force. Ohio EPA http://www.epa.state.oh.us/dsw/lakeerie/ptaskforce/LakeErieBackground.pdf Richards RP. Unpublished Data. Heidelberg University, Tiffin, Ohio. Rosa F, Burns NM. 1987. Lake Erie central basin oxygen depletion changes from 1929-1980. Journal of Great Lakes Research 13: 684-696.

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# Chesapeake Bay
Physical Description of the System
The Chesapeake Bay is a drowned river valley that is almost 300 km long with a relatively deep (20- 30 m) and narrow (1-4 km) central channel (Kemp et al. 2005) (Figure 1). Broad shallow areas flank this central channel over its entire length (Boicourt et al. 1999), and the mean depth of the estuary is 6.5 m (Kemp et al. 2005). Typically, the Bay is stratified with the surface freshwater isolating the deep and saline channel waters and limiting vertical mixing. Although occasionally strong wind events may cause some vertical exchange, stratification quickly returns due to the north-south salinity gradient (Boicourt 1992). There is a natural tendency for oxygen depletion in the deeper waters of the Bay due to the narrow and deep channel, persistent stratification, wide and shallow (and productive) sills flanking the channel, and a long water residence time (Boicourt 1992). Relative to other estuaries, the Chesapeake Bay watershed is large (172,000 km 2 

### ) compared to the size and volume of the estuary (Bricker et al. 1999). The shoreline is long (18,800 km) and many rivers drain the watershed into the Bay. The largest tributary is the Susquehanna River which accounts for 41% of the watershed. The Bay is closely connected to its watershed which delivers freshwater to the Bay at an average rate of 2,300 m 3 

### /s. Year-to-year fluctuations in river flow result in highly variable inputs of freshwater, nutrients, and sediment. The volume and nutrient composition of these flows influence stratification and productivity in the Bay (Kemp et al. 2005). This watershed is heavily populated, with an average density of 156 people per km 2 

### . In addition to the major population centers surrounding and connecting Baltimore and Annapolis, Maryland; Washington D.C.; and Richmond, Virginia; agriculture is a prominent feature of the watershed’s landscape (Bricker et al. 2007). 

# History of Hypoxia (issue, causes, economic and ecosystem impacts)
A recent assessment of eutrophic conditions in the Bay showed that it is among the most impacted estuaries in the United States. Nitrogen loads are high, and the Bay is susceptible to development of nutrient-related problems (Bricker et al. 2007). Eutrophic symptoms occur periodically or persistently and over extensive areas. These symptoms include high concentrations of chlorophyll- a , hypoxia, loss of submerged aquatic vegetation, nuisance and toxic algal blooms, and excessive growth of macroalgae. Throughout the Bay and its tributaries, symptoms have either worsened or not changed between 1999 and present (Bricker et al. 2007) (Figure 2). Primary sources of nutrients include agriculture, wastewater
, and urban runoff. Other sources include septic tanks, sewer overflows, and atmospheric deposition. The sources of nutrient inputs and their transport have been described in detail by Boynton et al. (1995) who determined that nitrogen and phosphorus inputs from the watershed and atmosphere increased by six toeight fold and 13 to 24 fold, respectively, between pre-colonial times and the mid-1980s. About oneFigure 1. Dissolved oxygen conditions in the Chesapeake Bay in 2005 http://www.chesapeakebay.net/ bayforecastspring2006.htm www.eco-check.org

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fourth of the nitrogen and one-third of the phosphorus are from point sources, with the rest from nonpoint terrestrial and atmospheric sources (see Chapter 1, Figure 3). Several seasonally varying factors influence the dynamics of oxygen depletion from bottom-waters of the Bay. The decline in dissolved oxygen in the spring appears to be controlled primarily by physical processes (e.g., stratification). Whereas, the late spring decline and the extent of summer hypoxia appear to be influenced by the level of phytoplankton production and by water temperature (Hagy et al. 2004), both of which influence the respiration rate. On an annual basis, the amount of freshwater inflow is a good predictor of bottom-water oxygen depletion, because it controls water column stratification and, therefore, the rate of oxygen replenishment through vertical mixing. Freshwater runof f also delivers nutrients, which fertilize phytoplankton growth. Year-to-year variations in runoff have also been correlated with production and sedimentation of organic matter (Boynton and Kemp 2000) and increased dissolved oxygen demand. Evidence from sediment studies in the Chesapeake Bay suggests that hypoxia has increased in occurrence over many decades (Kemp et al. 2005). Geochemical and paleontological methods were used to interpret this indirect ecological history (Brush 1984), and results suggest that eutrophication increased starting in the 17 th 

### century, coinciding with settlement by Europeans. Eutrophication accelerated during the 20 th 

### century as indicated by a pronounced shift in the ratio of planktonic to, typically , benthic diatoms, reflecting a decline in both water clarity and benthic algal production (Cooper and Brush 1993). Increases in fossil abundance of hypoxia-tolerant species (Karlsen et al. 2000), iron pyrite (Cooper and Brush 1991, 1993), and geochemical changes (Zimmerman and Canuel 2002) provide additional sedimentary evidence for development of hypoxia in the 20 th 

### century.

Figure 2. Volume of Chesapeake Bay affected by hypoxia/anoxia by year. http://www.chesapeakebay.net/bayforecastspring2006.htm www.eco-check.org

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Year-to-year variations in hypoxia extent and intensity are correlated with the amount of nitrogen entering the Bay. The amount of hypoxia generated from a given level of nitrogen loading has, however, been more severe in recent years relative to the past (Hagy et al. 2004). Since the early 1990s, the volume of hypoxic water has increased, while nitrogen loading has leveled or declined (Boesch et al. 2001, Hagy et al. 2004, Langland et al. 2006)). In the mid-1980s, there was a shift in the relationship between hypoxia and nitrogen loading, suggesting that the Bay has passed an ecological threshold and is now more susceptible to eutrophication processes. This shift may have reduced the capacity of natural processes to both oxygenate the water column and trap nutrients in the sediments (Kemp et al. 2005). Further compounding the effects of eutrophication has been the dramatic loss of oyster beds (currently biomass is approximately 1% of the 19 th 

### century levels, Newell 1988). Oysters strongly influence the Bay ecosystems through an ability to assimilate nutrients and rebound from hypoxic events (Kemp et al. 2005). Oysters reduce concentrations of phytoplankton and other suspended particles through filter

feeding, thereby increasing light levels reaching the sediment (e.g., Cohen et al. 1984, Newell and Koch 2004). The loss of oysters has reduced water clarity, which in turn has negatively impacted the growth of vital submerged aquatic vegetation. It has been estimated that at the height of ecosystem health, Bay waters were filtered once every four days, but the depleted filter feeder populations now require more than a year to filter the same volume (Newell 1988). Although heavy fishing pressure and other stressors in Chesapeake Bay make it difficult to isolate effects of eutrophication on fish, an increased ratio of pelagic (water column-dwelling) to demersal (bottom-dwelling) fish species documented for the Chesapeake Bay (Kemp et al. 2005) is indicative of increased eutrophication. Some of the most noticeable changes were the increase in pelagic Atlantic Menhaden, and the decline in demersal blue crab and oyster landings. This shift from demersaldominated to pelagic-dominated fisheries has been observed for other coastal systems and is attributed, in part, to bottom-water hypoxia (e.g., Caddy 1993). It is not clear how hypoxia influences the habitat requirements of particular fish and invertebrates, but some species, such as stur geons, can no longer reproduce or rear young in the Bay due to the lack of habitat with adequate oxygen and temperature levels (Niklitschek 2001, as cited in Kemp et al. 2005). Whereas impacts to fisheries have been difficult to determine, impacts of hypoxia on benthic animals have been well documented. The diversity, abundance, and productivity of many benthic animals is affected by seasonal hypoxia in the Bay, particularly in deeper water (Holland et al. 1987, Diaz and Rosenberg 1995), and the effective loss of habitat and fauna as a result of hypoxia can have profound effects on ecosystem energetics (Diaz and Rosenberg 2008). Estimates of biomass lost due to hypoxia are approximately 10,000 megatons of carbon per year, or 5% of the Bay’s total secondary production. Under healthy circumstances, an estimated 60% of benthic energy would be passed up the food chain in the Bay; therefore, it is estimated that hypoxic conditions in the Bay result in 6,000 megatons of carbon being lost as food energy for fisheries (Diaz and Rosenberg 2008). 

# Science and Management Actions (to date and planned)
Eutrophication and its influence on hypoxia have been studied extensively in the Chesapeake Bay in an effort to produce information useful for effective management of water quality and critical habitats (Boesch et al. 2001, Kemp et al. 2005). For example, Bay research has demonstrated that nitrogen and phosphorus limitation for phytoplankton growth vary seasonally and regionally (Fisher et al. 1999, Malone et al. 1986), underscoring the need to regulate both nitrogen and phosphorus inputs to the Bay

. In addition, studies in this system have shown that nitrogen removal from Chesapeake Bay through denitrification appears to be inhibited compared with estimates for other coastal ecosystems that do not experience seasonal hypoxia (Kemp et al. 1990). The Chesapeake Bay has also been subjected to long-

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term water quality monitoring, as well as a long-term benthic monitoring program that has been a key metric for assessing progress towards nutrient- and hypoxia-related goals. Historically, nutrient dynamics within the watershed have received less attention than in the Bay proper (Boesch et al. 2001), but there has recently been an increasing focus on the watershed processes. For example, it is clear that riparian zones, even in urban areas, are ‘hotspots’ of ecological function and can be effective at sequestering nutrients from runoff (Groffman et al. 2003). However, riparian zones can contribute to nitrogen loading in the Bay watershed when they under go the “urban stream syndrome” (Groffman et al. 2004), which occurs where impermeable surfaces increase runoff leading to channel incision and lower water tables, thereby reducing denitrification potential in the watershed. This information has contributed to ongoing water quality improvement ef forts. A reduction in eutrophication has been a top priority for management of the Chesapeake Bay in the last few decades (Boesch et al. 2001). Scientific research has improved public awareness and political interest in reversing eutrophication in the Chesapeake Bay (Malone et al. 1993), and a series of policies and adaptive management plans have evolved (Boesch et al. 2001). The Chesapeake Bay became the first estuary in the United States to be targeted by Congress for restoration and protection. An outgrowth of this recognition was the formation of a regional partnership of Bay states, the District of Columbia, and Federal agencies. An agreement to cooperatively work together to protect the Bay was codified in The Chesapeake Bay Agreement of 1983 (http://www.chesapeakebay.net/content/publications/cbp_12512. pdf). This agreement was signed by what would become the Chesapeake Executive Council, a group comprised of the governors of Maryland, Virginia, and Pennsylvania; the mayor of the District of Columbia; the administrator of the EPA; and the chair of the Chesapeake Bay Commission. In support of this agreement and the Chesapeake Bay Program, a Scientific and T echnical Advisory Committee was formed to provide the most recent and accurate information. In 1987, a second agreement was signed, pledging to reduce nitrogen and phosphorus inputs into the Bay by 40% by the year 2000. In 1992, amendments were added, reaffirming the original goals of the 1987 agreement, but also pledging the development of tributary-specific strategies to reduce nutrient inputs. Recognizing a lack of progress towards restoration of the Bay, members of the Chesapeake Bay Program drafted and signed the Chesapeake 2000 Agreement (C2K; http://www.chesapeakebay.net/ content/publications/cbp_12081.pdf), which provided comprehensive and specific direction for improving the water quality in the Bay, particularly related to nutrients. Recognizing the need to address Bay water quality within the watershed, the governors of Delaware, New York, and West Virginia entered into an agreement with the Chesapeake Executive Council to meet the goals of the C2K. This agreement was more aggressive than the previous one and called for, based on 1985 levels, a 48% reduction in nitrogen and 53% in phosphorus inputs. In 2003 and 2004, 36 tributary strategies were completed that outlined specific measures for each tributary to reduce the inputs of nutrients into the Bay

. A history of Chesapeake Bay eutrophication and the evolution of public policy and awareness are available in Boesch et al. (2001). Even more recently, in May 2009, President Obama issued Executive Order 13508 on Chesapeake Bay Protection and Restoration. To bring the full weight of the federal government to address the Chesapeake’s challenges, the Executive Order established the Federal Leadership Committee (FLC) for the Chesapeake Bay, which is chaired by the Administrator of the U.S. Environmental Protection Agency and includes senior representatives from the departments of Agriculture, Commerce, Defense, Homeland Security, Interior and Transportation. The EO charged the FLC with developing and implementing a new strategy for protection and restoration of the Chesapeake region. One of the primary strategies of the EO is “to restore clean water” in the bay, with the specific goal of reducing nitrogen, phosphorus, sediment

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and other pollutants to meet Bay water quality goals for dissolved oxygen, clarity , chlorophyll-a and toxic contaminants (http://executiveorder.chesapeakebay.net/). Research on nutrient cycling in soil-water systems of the Chesapeake Bay watershed provides fundamental insight into the movement of nutrients in agricultural systems and their impact on water resources. This research has led to the development of models for better predictions of nutrient transport via runoff as well as practical tools, such as the “Phosphorus Index” (adopted by all states in the Bay watershed), to guide nutrient management decisions on agricultural fields. The advent of Comprehensive Nutrient Management Planning has resulted in the development and implementation of best management practices (BMPs) for water and air quality protection. These BMPs range from traditional conservation practices—such as cover crops, riparian buffers, and constructed wetlands—to new technologies for precision application of fertilizers and manures to new practices for controlling and filtering drainage waters. Some of these BMPs can be transferred to nonagricultural uses, and ongoing research promises to deliver another generation of BMPs that immobilize nutrients and sediment. A recent assessment of water quality trends in rivers feeding the Bay showed significant improvements in loadings of nitrogen (72% of sites showed downward trends), total phosphorus (81% of sites), and sediment (43% of sites), indicating that management actions are having some ef fect in reducing nutrients and sediments (Langland et al. 2006). However, to date, lower nutrient input has not improved dissolved oxygen levels overall in the Chesapeake Bay, although it has caused small-scale reversals in hypoxia (Diaz and Rosenburg 2008). Clearly, there are complex and poorly understood mechanisms that are acting to delay recovery of some ecosystem components (e.g., seagrass beds), and these mechanisms are priorities for scientific attention. Restoration of seagrass, oyster , and marsh habitats are expected to help the Bay’s recovery from eutrophication and hypoxia by priming key ecological processes that will enhance recovery through biological positive-feedback mechanisms (Kemp et al. 2005). 

# Future Outlook
Small improvements in dissolved oxygen conditions are expected for the mainstem of the Chesapeake Bay, and the outlook for rivers draining into the Bay ranges from slight deterioration to slight improvement (Bricker et al. 2007). This projection is an improvement from the last eutrophication outlook presented in 1999 (Bricker et al. 1999), when the Bay and its tributaries were expected to experience small to large levels of deterioration. These projections are based on the prediction of expected nutrient load increases from wastewater treatment, septic tanks, agriculture, and urban runof f. Current potential management concerns include increasing nutrient loads from sources such as wastewater treatment, agriculture, urban runoff, atmospheric deposition, on-site septic tanks, and combined sewer overflow, particularly because an increase in population is expected in the watershed. To mitigate these potential problems, Bricker et al. (2007) suggests changes in land use policies that could limit urban sprawl and concentrate development, which could lead to more efficient treatment of runof f and waste.



# References
Boesch DF, Brinsfield RB, Magnien RE. 2001. Chesapeake Bay eutrophication: Scientific understanding, ecosystem restoration, and challenges for agriculture. J. Environ. Qual. 30: 303-320. Boicourt WC, Kuzmic M, Hopkins TS. 1999. The inland sea: circulation of Chesapeake Bay and the Northern Adriatic. In T. Malone, A. Malej, L. Harding, N. Smodlaka, R. Turner (eds.) Ecosystems at the land-sea margin: drainage basin to coastal sea. American Geophysical Union, Washington DC. P. 81-129.

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Boicourt WC. 1992. Influences of circulation processes on dissolved oxygen in the Chesapeake Bay . In: Smith DE, Leffler M, Mackiernan G (eds.) Oxygen dynamics in the Chesapeake Bay: a synthesis of recent researchy

. Maryland Sea Grant Publication, College Park, MD, p 7-59. Boynton WR, Garber JH, Summers R, Kemp WM. 1995. Inputs, transformations, and transport of nitrogen and phosphorus in Chesapeake Bay and selected tributaries. Estuaries 18: 285-314. Boynton WR, Kemp WM. 2000. Influence of river flow and nutrient loading on selected ecosystem processes and properties in Chesapeake Bay. In: Hobbie J. (ed.) Estuarine science: a synthetic approach to research and practice. Island Press, Washington, DC, p. 269-298.
Bricker SB, Clement CG, Pirhalla DE, Orlando SP, Farrow DRG. 1999. National Estuarine Eutrophication Assessment: effects of nutrient enrichment in the nation’s estuaries. NOAA, National Ocean Service, Centers for Coastal Ocean Science, Silver Spring, MD. Bricker S, Longstaff B, Dennison W, Jones A, Boicourt K, Wicks C, Woerner J. 2007. Effects of nutrient enrichment in the Nation’s Estuaries: A decade of change. NOAA Coastal Ocean Program Decision Analysis Series No. 26. National Centers for Coastal Ocean Science, Silver Spring, MD. 322 pp. Brush GS. 1984. Stratigraphic evidence of eutrophication in an estuary . W ater Resour. Res. 20: 531-541. Caddy JF. 1993. Toward a comparative evaluation of human impacts on fishery ecosystems of enclosed and semienclosed seas. Rev. Fish Sci 1: 57-95. Cohen RRH, Dresler PV, Philips EJP, Cory RL. 1984. The effect of the Asiatic clam Corbidula fulminea on phytoplankton of the Potomac River, Maryland. Limnol. Oceanogr. 29:170-18. Cooper SR, Brush GS. 1991. Long-term history of Chesapeake Bay anoxia. Science 254:992-996. Cooper SR, Brush GS. 1993. A 2500-year history of anoxia and eutrophication in Chesapeake Bay. Estuaries 16: 617-626. Diaz RJ, Rosenberg R. 1995. Marine benthic hypoxia: a refvfiew of its ecological ef fects and the behavioral responses of benthic macrofauna. Oceanogr. Mar. Biol. Annu. Rev. 33:245-303. Diaz RJ, Rosenberg R. 2008. Spreading dead zones and consequences for marine ecosystems. Science 321:926- 929. Fisher TR, Gustafson AB, Sellner K, Lacuture R, Haas LW, Magnien R, Karrh R, Michael B. 1999. Spatial and temporal variation in resource limitation in Chesapeake Bay. Mar. Biol. 133: 763-778 Hagy JD, Boynton WR, Keefe CW, Wood KV. 2004. Hypoxia in Chesapeake Bay, 1950-2001: Long-term change in relation to nutrient loading and river flow. Estuaries 27: 634-658. Holland AF, Shaughnessy AT, Hiegel MH. 1987. Long-term variation in mesohaline Chesapeake Bay macrobenthos: spatial and temporal patterns. Estuaries 10: 227-245. Groffman PM, Crawford MK. 2003. Denitrification potential in urban riparian zones. Journ. Environ. Qual. 32:1144- 1149. Groffman PM, Law NL, Belt KT, Band LE, Fisher GT. 2004. Nitrogen fluxes and retention in urban watershed ecosystems. Ecosystems 7: 398-403.
Karlsen AW, Cronin TM, Ishman ES, Willard DA, Holmes CW, Marot M, Kerhin R. 2000. Historical trends in Chesapeake Bay dissolved osygen based on benthic Foraminifera from sediment cores. Estuaries 23: 488-508. Kemp WM, Sampou P, Caffrey J, Mayer M, Henriksen K, Boynton WR. 1990. Ammonium recycling versus denitrification in Chesapeake Bay sediments. Limnol. Oceanogr. 35: 1545-1536. Kemp WM, Boynton WR, Adolf JE, Boesch DF, Boicourt WC, Brush G, Cornwell JC, Fisher TR, Glibert PM, Hagy JD, Harding LW, Houde ED, Kimmel DG, Miller WD, Newell RIE, Roman MR, Smith EM, Stevenson JC. 2005. Eutrophication of Chesapeake Bay: Historical trends and ecological interactions. Mar . Ecol. Prog. Ser . 303: 1-29. Langland MJ, Raffensperger JP, Moyer DL, Landwehr JM, Schwarz GE. 2006. Changes in Streamflow and Water Quality in Selected Nontidal Basins in the Chesapeake Bay Watershed, 1985-2004. Scientific Investigations Report 2006–5178 U.S. Department of the Interior, U.S. Geological Survey. 74p.

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Malone T, Kemp WM, Ducklow H, Boynton W, Tuttle J, Jonas R. 1986. Lateral variation in the production and fate of phytoplankton in a partially stratified estuary. Mar Ecol Prog Ser. 32: 149-160. Malone T, Boynton W, Horton T, Stevenson C. 1993. Nutrient loading to surface waters: Chesapeake case study. In: Uman MF (ed.) Keeping pace with science and engineering. National Academy Press, W ashington, DC p. 8-38. Newell RIE. 1988. Ecological changes in Chesapeake Bay: Are they the result of overharvesting the Eastern oyster (Crassostrea virginica)? In: Lynch MP, Krome EC (eds.) Understanding the estuary: advances in Chesapeake Bay research. Chesapeake Research Consortium Publication 129 (CBP/TRS 24/88) Bloucester Point, V A. pp. 536- 546. Newell RIE, Koch EW. 2004. Modeling seagrass density and distribution in response tochanges in turbidity from bivalve filtration and seagrass sediment stabilization. Estuaries 27: 793-806. Niklitschek EJ. 2001. Bioenergetics modeling and assessment of suitable habitat for juvenile Atlantic and Shortnose stugeons (Acipenser oxyrinchus and A. brevirostrum) in the Chesapeake Bay. PhD. Dissertation, University of Maryland, College Park, MD. Zimmerman AR, Canuel EA. 2002. Sediment geochemical records of eutrophication in the mesohaline Chesapeake Bay. Limn. Oceanogr. 47: 1084-1093.

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# Pensacola Bay
Physical Description of the System
The Pensacola Bay system is a network of estuarine bays located in the far western panhandle of Florida. The Bay system is arranged in two major arms that combine to form Pensacola Bay proper. The component bays include Escambia Bay, Blackwater Bay, East Bay, and Pensacola Bay (Figure 1). Santa Rosa Sound is relatively distinct shallow back-bay that joins the lower reach of Pensacola Bay and separates Santa Rosa Island from the mainland eastward to Destin, Florida, and Choctawhatchee Bay, Florida. The combined Pensacola Bay system, excluding Santa Rosa Sound, is medium sized (370 km 2 

### ) and shallow (mean depth is 3 m). Tides occur once per day and are relatively small, 15 to 65 centimeters. Three major watersheds drain into the Bay via the Escambia, Blackwater, and Yellow Rivers (Figure 1). 

# History of Hypoxia (issue, causes, economic and ecosystem impacts)
The condition of the Pensacola Bay system, particularly that of Escambia Bay , became a matter of public concern as early as the late 1960s after significant industrial point source dischar ges began and extensive fish kills and hypoxia were observed (Olinger et al. 1975). The lar ge fish kills prompted actions that ultimately led to elimination of the point source loadings by the mid-1970s. Olinger et al. (1975) provides a remarkable early compilation of ecological conditions in the Bay , intended principally to document the recovery of the system following reductions in industrial waste loads. These reports indicate that hypoxia was present in the Bays in the early 1970s, but does not provide enough data to evaluate the average extent or severity. EPA monitored the Bay quarterly from 1996-2001 (U.S. EPA 2005) and monthly from 2002- 2004 (Hagy and Murrell 2007). Whereas differences in survey methodology and data reporting mostly preclude quantitative analysis of ecological changes since the early 1970s, comparison with the most recent data suggests that neither recovery nor further degradation has occurred. The major ecological concerns of the early 1970s continue: bottom water hypoxia, loss of seagrass habitats, toxic contamination, and degradation of biotic communities (Hagy et al. 2008). Hypoxia occurs frequently and extensively in bottom waters of Pensacola Bay (Hagy and Murrell 2007). An average of 25% of the Bay bottom is affected during the summer. Moderate river flow conditions create the highest potential for hypoxia in the Bay. In 2004, nearly 40% of the Bay bottom was hypoxic for two consecutive months (Hagy et al. 2008). Strong winds can bring hypoxic water onto shallow shoals, giving hypoxia the potential to degrade nearly all habitats in the Bay . Hypoxia has not been observed in the lower Bay, south and west of Bayou Texar, where tidal exchange with the Gulf of Mexico appears to be adequate to prevent hypoxia. Figure 1. Map of Pensacola Bay, Florida.

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The causes of hypoxia in Pensacola Bay include strong salinity stratification, low tidal mixing ener gy, and sluggish estuarine circulation, which create an optimal physical environment for hypoxia (Hagy and Murrell 2007). On the other hand, water clarity in the Bay is often relatively high, chlorophyll -a concentrations are low to moderate, and phytoplankton production is moderate (Murrell et al. 2007). Correspondingly, rates of oxygen consumption in the water and sediments are relatively low (Murrell et al. 2009). These reflect low to moderate rates of nutrient loading, which can be related to low population density and minimal nutrient-intensive uses of much of the watershed (Hagy et al. 2008). The simultaneously low level of anthropogenic nutrient enrichment and eutrophication and high degree of hypoxia and seagrass loss suggests that the Bay has not been able to recover from earlier impacts. The impact of hypoxia on biological communities in Pensacola Bay has not been well documented, but one can infer from the extent, frequency, and severity of hypoxia that affected habitats are almost certainly in a poor ecological condition. Limited data from the Florida Inshore Monitoring and Assessment Program show that the numbers of benthic macrofauna in Pensacola Bay in 2003 were only 5 to 10% of numbers in healthier regions of the Florida west coast. Livingston (1999) found that substantial biomass of infaunal animals occurred in only a narrow zone around the perimeter of the Bay
, beyond the reach of persistent hypoxia. It has also been shown that chronic exposure to hypoxia imposed physiological stress on fish in Pensacola Bay, preventing them from reproducing (Thomas et al. 2007). Although the massive seagrass loss in Pensacola Bay has not been linked directly to hypoxia, it could reduce the abundance of animals that graze on algae growing on seagrasses, thus inhibiting their recovery

. For example, grass shrimp enhanced growth of Ruppia maritima , a seagrass that was once abundant in Pensacola Bay (McCall and Rakocinski 2007). Conceivably, the massive loss of seagrasses that has occurred and the extensive recurrent hypoxia could be mutually reinforcing, promoting persistence of these conditions despite currently unremarkable rates of nutrient loading. 

# Science and Management Actions (to date and planned)
The record of scientific research addressing water quality and ecological condition in Pensacola Bay began in earnest with the Olinger et al. (1975) studies. These studies documented many of the key features of the ecology of the Bay, including the incidence of hypoxia and some of its physical causes. Papers by EPA scientists, mostly since 2000, provide more detail on many aspects of the physical and biological conditions that control hypoxia in the Bay (see Hagy et al. 2008). EP A

will continue studies of Pensacola Bay as part of a regional research program in the northeast Gulf of Mexico. The project willfocus on providing data and methods to develop numeric water quality criteria for nutrients and nutrientrelated water quality parameters. Because Pensacola Bay is a relatively small estuary, no management programs have been created specifically to manage and improve its water quality (e.g., such as those created for Chesapeake Bay or the Gulf of Mexico). Florida’s Impaired Waters Rule provides some numeric guidelines for listing estuaries as impaired for chlorophyll- a and dissolved oxygen, but there are no enforceable water quality standards for nutrients or dissolved oxygen for the Bay. On the other hand, the state of Florida has recently increased its focus on developing water quality criteria for nutrients in its estuaries and coastal waters. Portions of Escambia Bay have been listed as impaired for an excessive increase in chlorophyll- a . Establishing levels for total maximum daily loads has been proposed and would call for significant reductions in both nitrogen and phosphorus loading to upper Escambia Bay. These actions signal an increase in regulatory attention to nutrients in Pensacola Bay. A continuation of scientific research by EPA and others could provide an improved scientific basis for these actions. Presently, the best hope for improved management of water quality in the Bay seems to be the likely prospect that the State of Florida will soon develop and adopt numeric nutrient criteria for

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Pensacola Bay. EPA is collaborating with the Florida Department of Environmental Protection to support its efforts to achieve this objective for Pensacola Bay and other estuaries in Florida. 

# Future Outlook
The relatively undeveloped status of a large portion of the watershed of Pensacola Bay is one of its best assets. Arguably, low population density in the watershed has prevented eutrophication and hypoxia from becoming much worse. However, the population of the coastal counties surrounding the Bay, especially Santa Rosa County, Florida, is projected to grow nearly 3.5-fold by 2060 (Zwick and Carr 2006), most likely increasing nutrient loading to this sensitive system. A regulatory approach utilizing the authority of the Clean Water Act and based on numeric nutrient criteria will be the best way of ensuring that this growth occurs in a manner that does not further degrade the Bay

. Although the available research clearly indicates that increased nutrients could harm water quality in the Bay , it is less clear that nutrient reductions alone will be sufficient to restore the Bay. A more active restoration program may be needed. Research is needed to determine the best methods for restoring ecological function in the Bay in order to simultaneously increase the extent of seagrass habitats, reduce hypoxia, and promote better biological condition overall. 

# References
Hagy JD III, Murrell MC. 2007. Susceptibility of a Gulf of Mexico estuary to hypoxia: An analysis using box models. Estuarine, Coastal and Shelf Science. 74: 239-253 Hagy JD III, Kurtz JC, Greene RM. 2008. An approach for developing numeric nutrient criteria for a Gulf coast estuary. U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Research Triangle Park, NC. EPA/600/R-08/004. 48 pp. Livingston RJ. 1999. Ecology and Trophic Organization, Section 5a, Volume 4 in Pensacola Bay System Environmental Study, unpublished report from Champpion International Corporation, 125 pp. McCall DD, Rakocinski CF. 2007. Grass Shrimp (Palaemonetes spp.) play a pivotal trophic role inenhancing Ruppia maritima. Ecology 88(3): 618-624. Murrell M C, Hagy JD III, Lores EM, Greene RM. 2007. Phytoplankton production in relation to nutrient distributions in a subtropical estuary: importance of freshwater flow. Estuaries and Coasts. 30(3): 390-402. Murrell MC, Campbell JG, Hagy JD III, Caffrey J. 2009. Effects of irradiance on benthic and water column processes in a shallow micro-tidal estuary: Pensacola Bay, Florida, USA. Estuarine, Coastal and Shelf Science 81: 501-512. Olinger LW, Rogers RG, Fore PL, Todd RL, Mullins BL, Bisterfield FT, Wise LA. 1975. Environmental and recovery studies of Escambia Bay and the Pensacola Bay system, Florida. Report No. 904/9-76-016, U.S. Environmental Protection Agency, Atlanta, Georgia. 468 pp. Thomas P. Saydur Rahman M, Khan IA, Kummer JA. 2007. Widespread endocrine diisruption and reproductive impainment in an estuarine fish population exposed to seasonal hypoxia. Proceedings of the Royal Society B. 274: 2693-2701. U.S. EPA. 2005. The Ecological Condition of the Pensacola Bay System, Northwest Florida (1994-2001). U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, Gulf Breeze, FL. EPA/620/R-05/002.
Zwick PD, Carr MH. 2006. Florida 2060, A population distribution scenario for the State of Florida. Geoplan Center, University of Florida, Gainsville, FL, USA. 29 pp.

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# Northern Gulf of Mexico
Physical Description of the System
The largest zone of oxygen-depleted coastal waters in the United States and arguably the second largest on Earth is in the northern Gulf of Mexico on the Louisiana continental shelf (Figure 1). The Gulf hypoxic zone typically occurs in waters 5-60 m in depth up to 125 km of fshore from west of the Mississippi River Delta and occasionally extending to the upper T exas coast. The dominant sources of freshwater, sediments, and nutrients to the northern Gulf of Mexico are the Mississippi and Atchafalaya Rivers. The watershed for these rivers (commonly referred to as the Mississippi Atchafalaya River Basin, or MARB) encompasses 41% of the contiguous United States (Figure 2). The average annual streamflow delivered from the MARB to the Gulf of Mexico during the period from 1981 to 2005 was 21,700 cubic meters per second (m 3 

### /s); the average annual flux of total nitrogen and total phosphorus during that period was 1.47 and 0.14 million metric tons, respectively (Aulenbach et al. 2007). Streamflow from the MARB enters the Gulf of Mexico through two deltas— about two thirds of the flow enters via the Mississippi River Birdfoot Delta (southeast of New Orleans, Louisiana), and one third via the Atchafalaya River Delta (200 km west on the central Louisiana coast).The freshwater discharge is carried predominantly westward along the Louisiana/Texas inner to midcontinental shelf, especially during peak spring discharge. This coastal ocean margin is characterized as a relatively shallow, open coastline with complex circulation patterns, weak tidal energies, generally high water temperatures, seasonally varying stratification strength, and lar ge inputs of freshwater that effectively result in an unbounded estuary, stratified for much of the year. Water column density stratification, which is critical to bottom water oxygen depletion, is dominated by vertical salinity gradients, but thermal warming of surface waters intensifies summer stratification strength. W ater column structure is also highly influenced by wind stress, frontal weather bands, hurricanes, and mixing of buoyant river plumes. 

# History of Hypoxia (issue, causes, economic and ecosystem impacts)

Extensive bottom water hypoxia forms each year between May and September. Since 1985 (when systematic mapping was started), the hypoxic area has averaged 13,808 km 2 

### and achieved its maximum size in 2002 at 22,000 km 2 

### (Figure 3). While low dissolved oxygen is commonly considered a bottom-water condition,oxygen-depleted waters often extend up into the lower half to two-thirds of the water column. Longterm increases in nutrient loads from the MARB, coupled with water column stratification, have been implicated as the primary causes of hypoxia (CENR 2000, U.S. EPA 2007). However, the complex physical, chemical, and biological processes along the coastal ocean mar gin—which consume, transform, and remineralize riverine nutrients and organic matter and ultimately result in bottom water oxygen depletion—remain poorly resolved in space and time. Figure 1. Map of bottom water oxygen levels in mg/L. Black line is less then 2 mg/L (hypoxic). Data source: N. Rabalais, Louisiana Universities Marine Consortium, NOAA, map by B. Babin.

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The nutrients delivered with freshwater inputs support primary productivity within the immediate vicinity of the river discharges as well as across the broader continental shelf. The settling of fixed carbon to the lower water column and seabed in the form of senescent phytoplankton, zooplankton fecal pellets, or aggregates provides a large carbon source for decomposition by aerobic bacteria, which in turn leads to hypoxia. Tropical storms, hurricanes, and cold front passages disrupt the hypoxia until stratification reestablishes and oxygen depletion processes continue.Low oxygen events on the LouisianaTexas continental shelf have been reconstructed over the past centuries using the relative abundance of low oxygen tolerant benthic foraminifera in sediment cores (Osterman et al. 2005). These records show that low oxygen events have increased over the past 50 years. Additionally, regression model hindcasts using historical Mississippi River discharge and nitrate concentrations indicated that large-scale hypoxia has likely been present along the continental shelf since the mid-1950s (Greene et al. 2009). More recently, the areal extent of the hypoxic zone increased from an average of 6,900 km 2 

### during 1985-1992 to 15,570 km 2 

### during 2005-2009 (Rabalais and Turner 2006, LUMCON 2009) (Figure 3). The increased prevalence of Gulf hypoxia over recent decades has been related to increases in nutrient loads (CENR 2000, U.S. EPA 2007). However, it has been demonstrated that hypoxia events have occurred for centuries driven by high streamflow events that flush nutrients from wetland ecosystems and stratify ocean waters (Osterman et al. 2005, Swarzenski et al. 2008). Alterations of the Mississippi and Atchafalaya Rivers for transportation and flood control over two centuries have significantly lessened the assimilation of nutrients in the watershed and changed the pattern of freshwater dischar ge to the coastal ocean margin (U.S. EPA 2007). Concurrent increases in anthropogenic inputs of nutrients to the watershed have contributed to increased eutrophication and hypoxia (from Bricker et al. 1998, Rabalais in Bricker et al. 2007). The region supports some of the most valuable commercial and recreational fisheries in the United States (Diaz and Solow 1999, Chesney et al. 2000, Zimmerman and Nance 2001). For example, T exas and Louisiana lead all states in catches of shrimp, which is the lar gest U.S. commercial fishery (NOAA 2007). However, partitioning the impacts of hypoxia on living resources from other ecosystem stressors (e.g., climate change and overfishing) has proven difficult and significant knowledge gaps remain. Fish kills are occasionally reported and significant impacts to benthic fauna have been well documented (e.g., Rabalais et al. 2001). Zimmerman and Nance (2001) suggest that severe hypoxic conditions may block the migration of shrimp from nearshore to offshore habitats. Additionally, brown shrimp are subjected to a significant amount of habitat loss due to hypoxia (Craig et al. 2005), congregating in suboptimal environments along the hypoxic zone edge, possibly causing a reduction in growth (Craig and Crowder 2005). Recent advancements in biomarker techniques have suggested that hypoxia may be causing Figure 2 Mississippi River Watershed with Dead Zone shown in Red (modified from Mississippi River/Gulf of Mexico Watershed Nutrient Task Force 2004).

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reproductive impacts in croaker as well (Thomas et al. 2007). 

# Science and Management Actions (to date and planned)
In 1998, the EPA established the Mississippi River/Gulf of Mexico Watershed Nutrient Task Force. The Task Force brought together Federal agencies, states, and tribes to consider options for reducing, mitigating, and controlling hypoxia in the northern Gulf of Mexico. In 1999 and 2000, the results of an integrated science assessment, requested by the T ask Force and conducted under the auspices of the National Science and Technology Council, was published (CENR 2000, Brezonik et al. 1999, Diaz and Solow 1999, Doering et al. 1999, Goolsby et al. 1999, Mitsch et al. 1999, and Rabalais et al. 1999) (http://oceanservice.noaa.gov/products/pubs_hypox.html). Using this science assessment, the Task Force published its first “Action Plan”, entitled Action Plan for Reducing, Mitigating, and Controlling Hypoxia in the Northern Gulf of Mex ico (Mississippi River/Gulf of Mexico Watershed Nutrient Task Force 2001), which was endorsed by Federal agencies, states, and tribal governments. The 2001 Action Plan coastal goal was to reduce the five-year running average size of the hypoxic zone to 5,000 km 2 

### by 2015 (Figure 3). It estimated that an overall reduction in nitrogen load of 30–45% would be required. The 2008 Plan identifies the requirement that phosphorus as well as nitrogen loads be reduced and increases the required reduction to at least 45%. The 2008 Plan also notes that reductions in nitrogen loads from 2001-2005 were from nitrogen forms other than nitrate, which is the primary form fueling spring primary production that leads to hypoxia. The Action Plan was based on a series of voluntary and incentive-based activities that address both reducing nutrient inputs and increasing assimilation in aquatic ecosystems, including proper timing and amount of fertilizer applications, best management practices on agricultural lands, restoration and creation of wetlands, river hydrology remediation, riparian buffer strips, nutrient removal from stormwater and wastewater, and coastal diversions. In 2007, an updated science assessment was conducted by the EPA Science Advisory Board under the oversight of the Task Force (U.S. EPA 2007). This update science assessment was used by the Task Force to develop the Gulf Hypoxia Action Plan for Reducing, Mitigating, and Controlling Hypoxia in the Northern Gulf of Mexico and Improving Water Quality in the Mississippi River Basin (Mississippi River/ Gulf of Mexico Watershed Nutrient Task Force 2008) (see Box 4 in Chapter 2). This plan retains the coastal goal of reducing the hypoxic zone to less than 5,000 km 2 

### by 2015, but understands the difficulty of meeting the goal. In this regard, the Task Force accepts the advice of the EPA Science Advisory Board on this issue: “The 5,000 km 2 

### target remains a reasonable endpoint for continued use in an adaptive management context; however, it may no longer be possible to achieve this goal by 2015... it is even more important to proceed in a directionally correct fashion to manage factors af fecting hypoxia than to wait for greater precision in setting the goal for the size of the zone. Much can be learned byFigure 3. Northern Gulf of Mexico hypoxic area by year, including 5 year running and longterm averages. Action Plan Goal is 5000 km 2

.

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implementing management plans, documenting practices, and measuring their ef fects with appropriate monitoring programs” (U.S. EPA 2007). The 2008 Action Plan, also like the 2001 Action Plan, calls for a reassessment after five years. The next reassessment will be conducted in 2013. On June 16, 2010, USDA released a report, “Effects of Conservation Practices on Cultivated Cropland in the Upper Mississippi River Basin.”(http://www.nrcs.usda.gov/technical/nri/ceap/umrb/index.html) This report, part of USDA’s Conservation Effects Assessment Project (CEAP), showed that agricultural producers in the Upper Mississippi River Basin have had great success in reducing soil erosion through widespread adoption of sediment controlling conservation activities. The assessment also showed that more widespread adoption of effective nutrient control practices is needed to meet the challenges associated with nitrogen and phosphorous leaching and runoff. USDA has committed $320 million to improve water quality in priority watersheds through its Mississippi River Basin Healthy W atersheds Initiative (MRBI). 

# Future Outlook
Although overall total annual nutrient loads to the northern Gulf from 2001–2005 suggests a decline in nitrogen inputs relative to the previous 24-year average (Mississippi River/Gulf of Mexico W atershed Nutrient Task Force 2008), reductions in nitrate during the critical spring period have not occurred. Further, the five-year running average (2005–2009) of the hypoxic zone is 15,570 km 2 

### , which is more than three times the Action Plan goal of 5,000 km 2 

### . The adaptive management approach put forth in the Action Plans of 2008 and 2001 commits to a reliance on sound science and continual feedback between the interpretation of new information and improved management actions as the key to tar geting actions within watersheds where they will be most effective. Significant variability in nutrient export rates, hypoxic zone size, and other parameters resulting from anthropogenic and/or climate change will make it difficult to assess the outcome of nutrient management actions. However , tar geted monitoring on representative watersheds, continued monitoring of streamflow and nutrient flux, and research that addresses biogeochemical processes and improved model application will provide the most ef fective means of measuring results and providing feedback on performance.



# References
Aulenbach BT, Buxton HT, Battaglin WA, Coupe RH. 2007. Streamflow and nutrient fluxes of the MississippiAtchafalaya River Basin and subbasins for the period of record through 2005: U.S. Geological Survey Open-File Report 2007-1080. http://toxics.usgs.gov/pubs/of-2007-1080/index.html Brezonik PL, Bierman VJ, Alexander R, Anderson J, Barko J, Dortch M, Hatch L, Hitchcock GL, Keeney D, Mulla D, Smith V, Walker C, Whitledge T, Wiseman WJ. 1999. Effects of reducing nutrient loads to surface waters within the Mississippi River Basin and Gulf of Mexico. Topic 4 Report for the Integrated Assessment on Hypoxia in the Gulf of Mexico. NOAA Coastal Ocean Program Decision Analysis Series No. 18. Silver Spring, MD: National Oceanic and Atmospheric Administration.
Bricker S, Clement C, Frew S, Harmon M, Harris M, Pirhalla D. 1998. NOAA ’ s Estuarine Eutrophication Survey. Volume 4: Gulf of Mexico Region. Silver Spring, MD. Office of Ocean Resources Conservation Assessment. 78 pp. CENR (Committee on Environment and Natural Resources). 2000. Integrated Assessment of Hypoxia in the Northern Gulf of Mexico. National Science and Technology Council Committee on Environment and Natural Resources, Washington, D.C. Chesney EJ, DM Baltz, RG Thomas. 2000. Louisiana estuarine and coastal fisheries and habitats: perspectives from a fish’s eye view. Ecological Applications 10: 350-366. Craig JK, Crowder LB. 2005. Hypoxia-induced habitat shifts and energetic consequences in Atlantic croaker and

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brown shrimp on the Gulf of Mexico shelf. Mar. Ecol. Prog. Ser. 294:79-94. Craig JK, Crowder LB, Henwood TA. 2005. Spatial distribution of brown shrimp(Farfantepenaeus aztecus) on the northwestern Gulf of Mexico shelf: effects of abundance and hypoxia. Canadian Journal of Aquatic Science 62:1295-1308. Diaz RJ, Solow A. 1999. Ecological and economic consequences of hypoxia—Topic 2, Gulf of Mexico hypoxia assessment: Silver Spring, MD, NOAA Coastal Ocean Program Decision Analysis Series, 86 pp., http:// oceanservice.noaa.gov/products/hypox_t2final.pdf Doering OC, Diaz-Hermelo F, Howard C, Heimlich R, Hitzhusen F, Kazmierczak R, Lee J, Libby L, Milon W, Prato T, Ribaudo M. 1999. Evaluation of economic costs and benefits of methods for reducing nutrient loads to the Gulf of Mexico. Topic 6 Report for the Integrated Assessment on Hypoxia in the Gulf of Mexico. NOAA Coastal Ocean Program Decision Analysis Series No. 20. Silver Spring, MD: National Oceanic and Atmospheric Administration. Goolsby DA, Battaglin WA, Lawrence GB, Artz RS, Aulenbach BT, Hooper RP, Keeney DR, Stensland GS. 1999. Flux and sources of nutrients in the Mississippi-Atchafalaya River Basin. T opic 3 Report for the Integrated Assessment on Hypoxia in the Gulf of Mexico. NOAA Coastal Ocean Program Decision Analysis Series No. 17. Silver Spring, MD: National Oceanic and Atmospheric Administration. Greene RM, Lehrter JC, Hagy JD III. 2009. Multiple regression models for hindcasting and forecasting midsummer hypoxia in the Gulf of Mexico. Ecological Applications 19(5): 1161-1175. LUMCON. 2009. ‘Dead Zone’ again rivals record size: Press Release, Louisianna Universities Marine Consortium (LUMCON), http://www.gulfhypoxia.net/research/shelfwidecruises/2008/PressRelease08.pdf Mississippi River/Gulf of Mexico Watershed Nutrient Task Force. 2001. Action Plan for Reducing, Mitigating, and Controlling Hypoxia in the Northern Gulf of Mexico. Washington, D.C.: Offices of Wetlands, Oceans, and Watersheds, U.S. Environmental Protection Agency. Mississippi River/Gulf of Mexico Watershed Nutrient Task Force. 2004. A Science strategy to support management decisions related to hypoxia in the Northern Gulf of Mexico and excess nutrients in the Mississippi River Basin: Prepared by the Monitoring Modeling and Research Workgroup of the Mississippi River/Gulf of Mexico Watershed Nutrient Task Force, U.S. Geological Survey Circular 1270, 58 pp. Mississippi River/Gulf of Mexico Watershed Nutrient Task Force. 2008. Gulf Hypoxia Action Plan 2008 for Reducing, Mitigating, and Controlling Hypoxia in the Northern Gulf of Mexico and Improving Water Quality in the Mississippi River Basin. Washington, D.C. Mitsch WJ, Day JW, Gilliam JW, Groffman PM, Hey DL, Randall GW, Wang N. 1999. Reducing nutrient loads, especially nitrate-nitrogen, to surface water, ground water, and the Gulf of Mexico. Topic 5 Report for the Integrated Assessment on Hypoxia in the Gulf of Mexico. NOAA Coastal Ocean Program Decision Analysis Series No. 19. Silver Spring, MD: National Oceanic and Atmospheric Administration. NOAA. 2007. Nutrient Enhanced Coastal Ocean Productivity (NECOP) Program: National Oceanic and Atmospheric Administration, Washington, DC. http://www.aoml.noaa.gov/ocd/necop Osterman LE, Poore RZ, Swarzenski PW, Turner RE. 2005. Reconstructing a180-yr record of natural and anthropogenic induced hypoxia from the sediments of the Louisiana continental shelf: Geology 33(4): 329–332. Rabalais NN, Turner RE, Justic D, Dortch Q, Wiseman WJ. 1999. Characterization of hypoxia. Topic 2 Report for the Integrated Assessment on Hypoxia in the Gulf of Mexico. NOAA Coastal Ocean Program Decision Analysis Series No. 15. Silver Spring, MD: National Oceanic and Atmospheric Administration. Rabalais NN, Harper DE Jr., Turner RE. 2001. Response of nekton and demersal and benthic fauna to decreasing oxygen concentrations, p. 115-128. In: NN Rabalais and RE Turner (eds), Coastal Hypoxia Consequences for Living Resources and Ecosystems. Coastal and Estuarine Studies 58, American Geophysical Union, W ashington D.C. Rabalais NN, Turner RE. 2006. Oxygen depletion in the Gulf of Mexico adjacent to the Mississippi River, in Neretin, L.N., ed., Past and present marine water column anoxia: NATO Science Series, IV-Earth and Environmental Sciences, Kluwer, pp. 225–245. Swarzenski PW, Campbell PL, Osterman LE, Poore RZ. 2008. A 1000-year sediment record of recurring hypoxia off the Mississippi River: the potnetial role of terrestrially-derived organic matter inputs. Marine Chemistry 109: 130- 142.

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Thomas P, Rahman MS, Khan IA, Kummer JA. 2007. Widespread endocrine disruption and reproductive impairment in an estuarine fish population exposed to seasonal hypoxia. Proc. Royal Society B-Biological Sciences 274: 2693-2702.

U.S. EPA. 2007. Hypoxia in the Northern Gulf of Mexico: An Update by the EPA Science Advisory Board: EPA-SAB08-004, December 2007, Washington, D.C., 275 pp. Zimmerman AR, Nance JM. 2001, Effects of hypoxia on the shrimp industry of Louisiana and Texas, In Rabalais, NN, Turner RE (eds.), Coastal hypoxia—Consequences for living resources coastal and estuarine studies: Washington, D.C., American Geophysical Union, chap. 15, v. 58, pp. 293–310.

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# Northeast Pacific Continental Shelf **(Oregon/Washington)**
Physical Description of the System The Oregon and Washington Shelf out to 200 meters water depth encompasses approximately 26,600 square kilometers (km 2 

### ) and has an average width of approximately 40 km. Generally, the shelf narrows from north to south, with a shelf width of more than 60 km off Washington and less than 20 km along southern Oregon. The exception to this general trend is a region called Heceta Bank in the central region of Oregon (43.8° to 44.6° N) which extends out to 65 km. The shelf is part of the Northern California Current system. This system has strong seasonal variability, with northerly (from the north) winds dominating from April to October, and southerly winds dominating through the winter. The strong northerly winds inducecoastal upwelling which brings nutrient-rich, lowoxygen waters onto the shelf (Figure 1). The major source of terrestrial input to the system is from the Columbia River, with an annual mean outflow of 6484 cubic meters per second (m 3 

### /s, 15 year mean from 1992 – 2006). River outflow tends to be low during the summer growing season and the input of nutrients from the river is negligible compared to contributions from upwelled waters (Hickey and Banas 2003). Thus, hypoxia off Washington and Oregon is unique in that it is due to natural sources rather than anthropogenic sources. Economically, upwelling-induced productivity in Oregon-Washington Shelf waters supports a $50 million fishery for dungeness crabs. This is a pot fishery, so crabs trapped in the pot fishing gear are killed during summertime hypoxic events. The value of the salmon fishery is on the order of $10-20 million; however, the impact of hypoxia on the landings of the crabs and salmon has not yet been evaluated economically. 

# History of Hypoxia (issue, causes, economic and ecosystem impacts)
Evidence of hypoxia on the Oregon-Washington continental shelf indicates that it is a seasonal occurrence (Figure 1) that has been present since at least the 1960s (Figure 2). However, there has not been a consistent sampling program developed to Figure 1. Seasonal change in bottom water oxygen concentration at a station 5 miles from shore off Newport, OR. The horizontal line at 1.4 ml/L represents hypoxic waters. Note that hypoxic waters appear only during the upwelling season (May-October). Source: Bill Peterson, NOAA NWFSC.

Figure 2. Oxygen concentration (ml/L) within 10m of the bottom at a station 5 miles offshore of Newport, OR. The thick, red line indicates hypoxic waters (1.4 ml/L). Although hypoxia was observed in the 1960s, it has been observed more frequently in this century. Source: Bill Peterson, NOAA NWFSC.

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assess the degree and magnitude of hypoxia over time (note the lack of any data between 1970 and 1998 in Figure 2). Data have been collected three to four times per year since 1998 during broad-scale surveys of zooplankton and salmon and biweekly since late 2005 (Figure 1). Cross-shelf transects (Figure 3) show that the hypoxic bottom waters can extend at least 20 to 30 meters off the bottom and occupy up to 30% of the water column. Low oxygen water moves onto the shelf in early spring (April or May) during the onset of upwelling. Throughout the summer, the oxygen level of bottom waters is reduced through the biological degradation of organic
matter. Wider regions of the shelf, where circulation patterns retain water for longer periods of time, tend to have more persistent and severe oxygen depletion (Figure 4). In September 2007, 8,600 km 2 

### of the shelfhad hypoxic bottom waters, covering 63% of the shelf area surveyed (Figure 4), making the OregonWashington shelf the second largest hypoxic region in the continental United States, second only to the Gulf of Mexico (which averages roughly 14,000 km 2 

### ). Severe hypoxia events were observed in 2002 (Grantham et al. 2004) and 2006 (Chan et al. 2008). From these events, it is clear that in some areas, such as Heceta Bank (just south of Newport), oxygen concentrations fall to very low values and can persist through much of the summer , ultimately killing or displacing nearly all of the bottom-dwelling organisms. Recent work on the impacts of hypoxic waters on the development of copepods indicate that oxygen concentrations below 1 ml/L (or 1.43 mg/L) greatly reduces the hatching rate of copepod eggs (Peterson unpublished). In instances where copepods are not able to move out of hypoxic zones, the reduced hatching rate may also be accompanied by reduced

Sept 1998 Sept 1999 Sept 2000 Sept 2001

Sept 2002 Sept 2003 Sept 2004 Sept 2005

Sept 2006Depth (m)

Longitude (W) Sept 2007 Sept 2008

Figure 3. Oxygen concentration along a 46 km (25 mile) transect across the Oregon shelf (NH Line of f Newport, Oregon). The thick, red contour indicates the region of hypoxia (1.4 ml/L). The gray shaded area is the bottom bathymetry. Source: Jay Peterson, NOAA/OSU CIMRS.

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egg production (Marcus 2001, Sedlacek and Marcus 2005), severely impacting recruitment of species critical to the food web of many commercially important fish species. The cause of the hypoxia is driven primarily by an interaction between circulation and biological activity. The typical upwelling season extends from April to October. During this time, periods of strong winds from the north bring nutrient-rich, low-oxygen waters onto the shelf. The nutrient-rich waters are mixed into the surface layer and fuel a highly productive planktonic food chain. However , vertical transport of fecal pellets of grazers and sinking of nutrient-depleted phytoplankton blooms results in the degradation of massive amounts of organic matter on the sea floor. Depending on the vertical flux and on the retention time of the water mass, the oxygen consumption can rapidly lead to hypoxic and even anoxic conditions. 

# Science and Management Actions (to date and planned)
To date, no specific scientific programs have been established to monitor the status of hypoxic zones along the Oregon-Washington shelf. The development of hypoxic regions along the coast is primarily driven by naturally occurring physical and biological processes. Changes in the amount of nutrients upwelled to surface waters, water temperatures, and retention of water masses on the shelf likely contribute to the persistence, size, and severity of hypoxic zones. The low oxygen zones have been shown to greatly impact bottom-dwelling fishes, shellfish, and other or ganisms inhabiting the shallow banks (i.e., Heceta Bank). 

# Future Outlook
A coordinated research and monitoring effort aimed at informing resource managers is needed. The combination of increased awareness of the spatial extent of hypoxia, its impact on the shelf ecosystem, Figure 4. Distribution and extent of hypoxic waters (bold 1.4 ml/L contour) along the Oregon-Washington shelf. Shown is the minimum oxygen value regardless of the depth it occurred (but the minimum values was almost always at the deepest sampling). Panels show the extent in September 2006 (far left), and the seasonal progression during 2007 (May , June and Sept). The dashed line along the coast represents the 200 m isobath. Data Source: Cheryl Morgan (OSU and CIMRS) and Bill Peterson (NOAA NWFSC), project supported by Bonneville Power Administration.

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and the availability of instruments that can easily be integrated into current scientific programs will provide additional data on the timing and extent of hypoxia along the Oregon-W ashington shelf. This improved understanding may also lead to an ability to forecast the extent and severity of low dissolved oxygen along the shelf which could be a useful tool for fishery managers. Research programs—such as those carried out through the Pacific Coastal Ocean Observing System (NOAA), the Partnership for Interdisciplinary Studies of Coastal Oceans (Oregon State University , et al.), the Oregon Coastal Ocean Observing System (Oregon State University), the Olympic Coast National Marine Sanctuary (NOAA/National Marine Sanctuaries), and the Bonneville Power Administration (Oregon State University, NOAA)—collect hydrographic data, including dissolved oxygen, physical, and biological data along the Oregon-Washington shelf. Combined with regional and broad-scale hydrographic data, it will be possible to gain a better understanding of the potential mechanisms driving the variability of the hypoxic zone. Understanding how natural versus anthropogenically induced changes in climate influence the spatial extent and severity of hypoxic zones along the shelf requires consistent, long-term observations across the entire region of interest. 

# References
Chan F, Barth JA, Lubchenco J, Kirincich A, Weeks H, Peterson WT, Menge BA. 2008. Emergence of Anoxia in the California Current Large Marine Ecosystem. Science 319:920 Grantham BA, Chan F, Nielsen KJ, Fox DS, Barth JA, Huyer A, Lubchenco J, Menge BA. 2004. Upwelling-driven nearshore hypoxia signals ecosystem and oceanographic changes in the northeast Pacific. Nature 429:749-754 Hickey BM, Banas NS. 2003. Oceanography of the U.S. Pacific Northwest coastal ocean and estuaries with application to coastal ecology. Estuaries 26:1010-1031 Marcus NH. 2001. Zooplankton: Responses to the consequences of hypoxia. In: Rabalais NN, T urner RE (eds) Coastal and Estuarine Studies: Coastal Hypoxia, Vol 58. American Geophysical Union, Washington D.C., p 49-60
Peterson WT. Unpublished data. National Oceanic and Atmospheric Administration, Northwest Fisheries Science Center. Sedlacek C, Marcus NH. 2005. Egg production of the copepod Acartia tonsa : The influence of hypoxia and food concentration. J Exp Mar Biol Ecol 318:183-190.

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# Yaquina Bay
Physical Description of the System
Yaquina Estuary is a small, drowned, river valley estuary located along the central Oregon coast of the United States (Figure 1) with an estuarine surface area of 19 square kilometers (km 2 

### ) and a watershed area of 650 km 2 

### (Lee et al. 2006). Approximately 48% of the estuarine area is intertidal. November through April (wet season) is a period of high precipitation along the Oregon coast when the estuary is river dominated. From May through October (dry season) the estuary switches from riverine to marine dominance and a saltwedge extends fairly far upriver. Two tributaries, the Yaquina River and Big Elk Creek, with similarly sized drainage areas, contribute approximately equally to the long-term median freshwater inflow of 7.5 cubic meters per second (m 3 

### /s). The estuary is well mixed under low flow conditions, and partially- to well-mixed during winter high inflow conditions. T ides are semidiurnal with a mean tidal range of approximately 1.9 m (Shirzad et al. 1989). The estuary is divided into two zones, one of which is dominated by ocean input (Zone 1) and the other which is more influenced by watershed and point source inputs (Zone 2). Due to the small volume of the estuary and the strong tidal forcing, there is close coupling between the estuary and the coastal ocean. Approximately 70% of the volume of the estuary is exchanged with the coastal ocean during each tidal cycle (from Brown et al. 2007, Bricker et al. 1998). Like other estuaries in the Pacific Northwest that are adjacent to the California Current System, Y aquina Estuary is influenced by seasonal wind-driven upwelling which moves relatively cool, nutrient-rich water into the estuary during April through September. 

# History of Hypoxia (issue, causes, economic and ecosystem impacts)
The Yaquina watershed is heavily forested with deciduous, evergreen, and shrub land-use classes constituting 85% of the watershed (Lee et al. 2006, based on NOAA 2001 Coastal Change Analysis Program data). Although primarily forested and showing little “urban footprint,” the watershed has been impacted by a variety of disturbances, including logging which began in the mid-1800s and continues to the present. In addition to the direct effects of logging on erosion and water quality, rafting of logs can also affect aquatic habitats by physical disturbance, alteration of flow regimes, and accumulation of wood and bark debris and sawdust which can smother the benthos and result in low dissolved oxygen and/or elevated hydrogen sulfide (Sedell et al. 1991). There are three other nutrient sources influencing low dissolved oxygen in the Yaquina Estuary: sewage from municipal discharges, industrial discharges, and nonpoint sewage inputs specifically from septic systems. The Yaquina Estuary watershed contains Figure 1. Location map of Yaquina Estuary in Oregon. The estuary is divided into “marine dominated” (Zone 1) and “riverine dominated” (Zone 2) segments (Lee et al. 2006) based on the relative proportion of oceanic-derived nutrients versus terrestrially derived nutrients (from Brown et al. 2007).

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the cities of Toledo and Newport; however, only the “Bay Front” of Newport lies within the watershed boundaries. Although the National Estuarine Eutrophication Assessment (Bricker et al. 2007) reported that Yaquina has only low level problems with dissolved oxygen, there are strong seasonal patterns within the estuary (Figure 2). Oxygen levels in the estuary are comparatively stable during the wet season, but show a decline during the dry season. The wet season dissolved oxygen values have an overall mean of 9.7 mg/L, which is well above the threshold considered to be hypoxic, indicating no significant problems. The dry season data show a decline to an overall mean value of 5.8 mg/L but it increases again to wet season values. Zone 1 (marine-dominated) and Zone 2 (riverine-dominated) follow the same pattern, but Zone 2 appears to have the lower values overall. The lowest values during the dry season are approaching hypoxic conditions (< 2 mg/L). However, even during the dry season, most values are above the threshold considered to be hypoxic, indicating minimal problems with dissolved oxygen in this system. During 1960–1984, there was a noted improvement in dissolved oxygen concentrations in Zone 2 (riverine-dominated), but it is not clear if the cause of the observed trend was from the decrease in logging or the improved sewage treatment. Recent (2002-2006) dissolved oxygen levels in Zone 2 are similar to dissolved oxygen levels during the mid-1980s, suggesting that there have been no recent changes in dissolved oxygen levels. Since 2002, there has been an increase in the incidence of hypoxic events on the Oregon shelf (Grantham et al. 2004) that have the potential to influence dissolved oxygen levels within the estuary
, especially during periods of low oxygen water upwelling (and particularly in Zone 1). Data collected 3.7 km from the mouth of the estuary show that hypoxic shelf water is imported into Y aquina Estuary during

Figure 2. Seasonal pattern (1960-1984) of dissolved oxygen at all locations in the Yaquina Estuary and River with squares and triangles representing samples from Zones 1 (oceanic dominated) and 2 (river dominated), respectively . Solid line is nonlinear least-squares fit to data, which was modeled as a constant during wet season and a cosine function of date during the dry season (n = 869; from Brown et al. 2007).

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flood tides. A time series of dissolved oxygen and salinity data measured during July 9-19, 2002, coinciding with a documented hypoxic event on the Oregon shelf off of Newport, Oregon (Grantham et al. 2004), shows the import of hypoxic shelf water into the estuary (Figure 3a). Minimum dissolved oxygen levels occurred during maximum salinities, coincident with flooding tides. In addition, minimum dissolved oxygen levels occur during minimum water temperatures (~ 9 degrees C), which is indicative of recently upwelled water. These results show that, like other Pacific Coast estuaries, dissolved oxygen conditions in the lower portion of the estuary are strongly influenced by ocean conditions due to close coupling between the shelf and the estuary resulting from strong tidal forcing and upwelling during the wet season. Although this has been reported with increasing frequency recently, it is not a new phenomenon. A study of the Yaquina Estuary attributed low dissolved oxygen concentrations (5 mg/L) in the lower estuary to coastal upwelling during July 1968 (Gibson 1974). 

# Science and Management Actions (to date and planned)
In response to observed problems with sewage in the early 1900s, a combined stormwater/sewage system that discharged raw sewage into the Yaquina River was constructed in Toledo in 1926, and then upgraded in 1954 to a primary treatment facility to handle the municipal waste from the city of T oledo. This facility, which discharges into the Yaquina Estuary (about 22 km from the mouth of the estuary), was upgraded to secondary treatment in 1981. In the late 1980s and early 1990s, the city of T oledo made improvements to their stormwater collection system, reducing the bypassing of the treatment plant during high flow periods. In 1996, the Toledo plant had a discharge of 0.979 million gallons per day with a design capacity of 3.5 million gallons per day (www.epa.gov/OW-OWM.html/mtb/cwns/1996report2/ or.htm). In addition to the Toledo municipal discharge, a number of houses along the Yaquina Estuary and River have on-site septic systems, some which were previously failing. The primary environmental impact of these septic systems appears to be microbial contamination and they have all been repaired. A combined sewage discharge with a pump station was constructed for Newport in the mid-1950s, which eliminated the direct discharge of sewage from Newport into Yaquina Estuary (Brown et al. 2007). A municipal sewage system with primary treatment and an offshore discharge was constructed in Newport in 1964, which has since been upgraded to secondary treatment. Addressing issues associated with ocean input of nutrients and the seasonal shift in dominance of riverine and oceanic loading is critical in the process of developing nutrient criteria for estuaries in the Pacific Northwest region. Several studies have been conducted to try to understand sources and variability in order to develop appropriate nutrient criteria and management measures. Figure 3. a) Time-series of dissolved oxygen and salinity and b) salinity versus dissolved oxygen showing import of hypoxic ocean water at a station 3.7 km from mouth of estuary (from Brown et al. 2007).

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# Future Outlook
Although presently hypoxia is not a significant problem in this estuary , it may worsen in the future since the population of Lincoln County, where Toledo and Newport are located, is predicted to increase by 12% by 2020. It is possible that conditions will remain the same, if wastewater treatment and other land-based nutrient management measures are maintained and improved. However , hypoxia and other nutrient-related problems may worsen due to the additional nutrient loads associated with the population increase if nutrient management infrastructures are overloaded. This is particularly true in Zone 2 (riverine-dominated), since this zone is more influenced by land-based sources of nutrients than Zone 1. Historical reduction of dissolved oxygen in Zone 2 suggests that this system can be impacted by watershed activities even in the presence of strong flushing. However , if the population of Newport increases, water quality in the lower estuary may also worsen as a result. The import of low dissolved oxygen water into the estuary from the coastal ocean may result in this system being susceptible to hypoxia in the future. 

# References
Bricker S, Longstaff B, Dennison W, Jones A, Boicourt K, Wicks C, Woerner J. 2007. Effects of Nutrient Enrichment in the Nation’s Estuaries: A Decade of Change, National Estuarine Eutrophication Assessment Update. NOAA Coastal Ocean Program Decision Analysis Series No. 26. National Centers for Coastal Ocean Science, Silver Spring, MD. 322 pp. http://ccma.nos.noaa.gov/news/feature/Eutroupdate.html Bricker S, Clement C, Frew S, Harris M, Pirhalla D. 1998. NOAA’s Estuarine Eutrophication Survey. Volume 5: Pacific Coast Region. Silver Spring, MD. Office of Ocean Resources Conservation Assessment. 75 pp. Brown CA, Nelson WG, Boese BL, DeWitt TH, Eldridge PM, Kaldy JE, Lee II H, Power JH, Young DR. 2007. An Approach to Developing Nutrient Criteria for Pacific Northwest Estuaries: A Case Study of Yaquina Estuary, Oregon. USEPA Office of Research and Development, National Health and Environmental Effects Laboratory, Western Ecology Division. EPA/600/R-07/046. Gibson GC. 1974. Oyster mortality study- summary report 1966-1972. Fish Commission of Oregon, Management and Research Division. United States Department of Commerce, National Marine Fisheries Service. 37 pp. Grantham BA, Chan F, Nielsen KJ, Fox DS, Barth JA, Huyer A, Lubchenco J, Menge BA. 2004. Upwelling-driven nearshore hypoxia signals ecosystem and oceanographic changes in the northeast Pacific

. Nature 429:749-754. Lee H. II, Brown CA, Boese BL, Young DR (eds.). 2006. Proposed Classification Scheme for Coastal Receiving Waters Based on SAV and Food Web Sensitivity to Nutrients, Volume 2: Nutrient Drivers, Seagrass Distributions, and Regional Classifications of Pacific Northwest Estuaries, United States Environmental Protection Agency Report, Office of Research and Development, National Health and Environmental Ef fects Laboratory . Internal Report. Sedell JR, Leone N, Duval WS. 1991. Water Transportation and Storage of Logs. In: Influences of Forest and Rangeland Management on Salmonid Fishes and Their Habitats. American Fisheries Society Special Publication 19:325-368. Shirzad FF, Orlando SP, Klein CJ, Holliday SE, Warren MA, Monaco ME. 1989. National estuarine inventory: Supplement 1, Physical and Hydrologic characteristics, The Oregon estuaries., National Oceanic and Atmospheric Administration, Rockville, MD.

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# Hood Canal

Physical Description of the System
Despite its name, Hood Canal is a natural formation which has experienced hypoxia periodically dating back centuries (Brandenberger et al. 2009). It is a sub-basin of Puget Sound, Washington, with a fjord-like structure (Figure 1), including a natural sill at the mouth which restricts circulation with greater Puget Sound; it has a surface area of 386 square kilometers (km 2 

### ) (King County 2001). Restricted and slow circulation coupled with a strong salinity stratification and high productivity make it conducive to low dissolved oxygen conditions (Newton 2007). 

# History of Hypoxia (issue, causes, economic and ecosystem impacts)
Hypoxic events have increased in intensity, duration, and spatial extent since the 1990s, causing fish kills that prompted the Washington State Department of Fish and Wildlife to close many fisheries in Hood Canal in 2003. Southern Hood Canal, towards Lynch Cove (Figure 2), has experienced more hypoxia than the rest of the canal, and the measured dissolved oxygen concentrations are now lower in this area than they were in the 1950s and 1960s (Newton 2007). Hypoxia also develops seasonally along the main stem of Hood Canal, and transient upwelling of these waters caused by wind events has caused fish kills that garnered considerable public attention. Mortality events of living resources have been reported back to the 1920s (Fagergren et al. 2004). Ecosystem impacts such as these, however, have increased in frequency since 2002 including an extensive event in September 2006 (Newton 2007). Nitrogen inputs, especially from the Lynch Cove area, have been implicated, but nitrate from inflowing seawater (Paulson et al. 2006), changes in circulation and flushing (e.g., modified river flows), stratification, and algal blooms are also important (HCDOP 2007). 

# Science and Management Actions (to date and planned)
As a result of heightened attention to Hood Canal environmental deterioration, two significant developments may lead to improvements in hypoxia in the future. First, the Hood Canal Dissolved Oxygen Program (http://www. hoodcanal.washington.edu/index.jsp), a partnership of 28 organizations, including NOAA, EPA, USGS, USACE, and the U.S. Navy, was created in 2005 to study oxygen dynamics through broad-scale community involvement, concerted assessments, and development of models. In

addition to the Hood Canal Dissolved Oxygen Program, but operating on a larger scale, the Puget Sound Partnership (http://www.psp.wa.gov/), a new state agency, was Figure 1. Diagram of Cross Section of Hood Canal with prevailing geography and physical conditions (Newton 2007).

Figure 2. August 2006 interpolation reflecting typical pattern of low DO concentrations in Hood Canal (Newton 2007).

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established in 2007 to develop a coordinated, region-wide response to the deterioration of the Sound. The group adopted an ambitious action plan in 2008. 

# Future Outlook
The factors driving hypoxia in Hood Canal are still being clarified, especially the role of nutrients derived from human activities. Creation of the Puget Sound Partnership in 2007—which represents a broad coalition of citizen, Federal, state, and tribal agencies and interests—should allow development of deliberate protection actions for Puget Sound, and Hood Canal specifically. It is important to develop a comprehensive management plan before the situation worsens given the expected growth in the coastal population around Puget Sound. 

# References
Brandenberger JM, Louchouarn P, Crecelius EA. 2009, submitted . Historical reconstruction of redox sensitive metals in two basins of Puget Sound: Evaluating Natural and Post Urbanization Signatures of Hypoxia. Submitting to Geochimica et Cosmochimica Acta. Fagergren D, Criss A, Christensen D. 2004. Hood Canal low dissolved oxygen: Preliminary assessment and corrective action plan: Olympia, Washington, Puget Sound Action Team, Publication #PSAT04-06, 43 pp. plus appendices. HCDOP (Hood Canal Dissolved Oxygen Program). 2007. http://www.hoodcanal.washington.edu/observations/ historicalcomparison.jsp King County. 2001. Features Of Puget Sound Region: Oceanography And Physical Processes, Chapter 3 of the State of the Nearshore Report, King County Department of Natural Resources, Seattle, W A, 2001. Newton JA. 2007. Hood Canal, WA: The complex factors causing low dissolved oxygen events require ongoing research, monitoring, and modeling. In: Bricker S, Longstaff B, Dennison W, Jones A, Boicourt K, Wicks C, Woerner J. 2007. Effects of Nutrient Enrichment in the Nation’s Estuaries: A Decade of Change, National Estuarine Eutrophication Assessment Update. NOAA Coastal Ocean Program Decision Analysis Series No. 26. National Centers for Coastal Ocean Science, Silver Spring, MD. 322 pp. http://ccma.nos.noaa.gov/news/feature/Eutroupdate.html Paulson AJ, Konrad CP, Frans LM, Noble M, Kendall C, Josberger EG, Huffman RL, Olsen TD. 2006. Freshwater and saline loads of dissolved inorganic nitrogen to Hood Canal and Lynch Cove, western Washington: U.S. Geological Survey Scientific Investigations Report 2006-5106, 92 pp.

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State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Great Lakes Region Michigan Lake Huron, Central Basin None 1990 Ahrnsbrak and Wing 1998 Michigan Lake Michigan, Central Basin None 1990 Ahrnsbrak and Wing 1998 Michigan Lake Superior, Central Basin None 1990 Ahrnsbrak and Wing 1998 New York Lake Ontario, Kingston Basin Documented 1990 Ahrnsbrak and Wing 1998 New York Lake Ontario, Main Basin None 1990 Ahrnsbrak and Wing 1998 New York Lake Ontario, Prince Edward Bay Concern 1990 Ahrnsbrak and Wing 1998

Ohio Lake Erie, Central Basin Documented 1700 Suitable whitefish habitat reduced by low dissolved oxygen. Benthos reduced. Periodic hypolimnetic anoxia prior to European settlement. Global warming will lower dissolved oxygen from higher microbial metabolism. Increase in anoxia from 1930s to 1970s. Hartman 1972, Krieger 1985, Rosa and Burns 1987, Delorme 1982, Edwards et al. 2005, Blumberg and DiToro 1990, Krieger et al. 2007 Ohio Lake Erie, Eastern Basin None 1990 Ahrnsbrak and Wing 1998 Ohio Lake Erie, Western Basin Documented 1950 Mortality of mayflies in 1953. Mayfly populations partially recovered in 1990s. Occasional hypoxia, improved in 1990s. Britt 1955, Krieger 1985, Gerlofsma and Ciborowski 1999



### Gulf of Mexico Region

Alabama Alabama Inner Shelf Documented 1990 Area off Dauphin Island hypoxic. Dissolved oxygen declined from 1980s to 1990s. Rabalais et al. 1985, Rabalais 1998, Byrnes et al. 1999 Alabama Bon Secour Bay Documented 2000 Mortality of benthos and loss of oysters. Rikard et al. 2000 

# Appendix III. Table of U.S. Systems **Impacted by Hypoxia**

Table A1. Systems in the United States by region and their dissolved oxygen condition. Regions are based on Bricker et al. 2007. Analysis current through December 2008. Documented indicates systems with low dissolved oxygen measurements leading to hypoxia and evidence that hypoxia was caused, at least in part, by human activity—mainly related to eutrophica

tion, but also hydraulic modifications such as dams and canal construction. Concern indicates systems that exhibit primary or secondary effects of eutrophication, including elevated nutrient levels, elevated chlorophyll levels, or harmful algal blooms. These systems are impaired by nutrients and are possibly at risk of developing hypoxia. Some of the systems classified as areas of concern may already be experiencing hypoxia, but there is a lack dissolved oxygen data. None indicates systems with no measured low dissolved oxygen and with low expression of primary or secondary effects of eutrophication.

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 119 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Alabama Meaher Park Documented 2000 Data is from water quality monitoring stations. Mobile Bay NEP 2008 Alabama Mobile Bay Documented 1860 Occasional shoreward migration of hypoxia stressed fish and crustaceans (Jubilees). May have been hypoxic in 1800s. Has winter hypoxia. Stratification a major factor. Some improvement in dissolved oxygen between 1900s and 2000s. Pennock et al. 1994, May, 1973, Loesch 1960, Engle and Summers 1999, Rabalais et al. 1985, Bricker et al. 1999, 2007 Alabama Mobile River Concern 1980 Rabalais et al. 1985 Alabama Tensaw River Concern 1980 Rabalais et al. 1985 Alabama Weeks Bay Documented 1990 Mangrove area. Sanger et al. 2002 Alabama Wolf Bay Documented 1980 Part of regional water quality monitoring program. Engle et al. 1999, Rabalais et al. 1985 Florida Alafia River Documented 1980 Windsor 1985 Florida Alaqua Creek None 1980 Windsor 1985 Florida Allen Creek Concern 1980 Windsor 1985 Florida Alligator Alley Canal Documented 1970 Windsor 1985

Florida Alligator Creek Concern 1980 Windsor 1985

Florida Apalachee Bay Documented 1970 Localized hypoxia and anoxia in 1970s attributed to pulp mills in Econfina and Fenholloway Rivers, some dissolved oxygen improvements from1990s to 2000s. Windsor 1985, Bricker et al. 1999, Heck 1976, Livingston 1975

Florida Apalachicola Bay Documented 1990 No hypoxia in 1980s, observed in 1990s. Some improvement in dissolved oxygen in 2000s. Lowery 1998, Sanger et al. 2002, Windsor 1985, Bricker et al. 1999 Florida Apalachicola River None 1980 Windsor 1985

Florida Aucilla River Concern 1980 Windsor 1985 Florida Bayou Chico Documented 1990 Summers et al. 1997 Florida Bayou Marcus Creek Documented 1980 Windsor 1985

Florida Bear Creek Concern 1980 Windsor 1985 Florida Big Lagoon Documented 1990 Not hypoxic in 1980s. Part of regional water quality monitoring program. Whitledge 1985, Engle et al. 1999

Florida Big Lostman's Bay Concern 1980 Windsor 1985

Florida Blackwater River None 1980 Windsor 1985 Florida Caloosahatchee River Documented 1990 Windsor 1985, Bricker et al. 1997, 2007 Florida Carpenters Creek/Bayou Texar Concern 1980 Windsor 1985

Florida Carrabelle River None 1980 Windsor 1985

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 120 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Florida Charlotte Harbor Documented 1980 1980s to 1990s improvement in water quality from nutrient management, 2000s decline in water quality as population expanded. Dissolved oxygen declined from 1970s to 1990s, than increased to 1970s levels by end of 1990s, declined again in 2000s. Associated wet season and stratification, hurricane disturbance resulted in more low dissolved oxygen. Tomasko et al 2006, Turner et al. 2006, Windsor 1985

Florida Chatham Bay/ River Concern 1980 Windsor 1985

Florida Choctawhatchee Bay Documented 1980 Some improvement in dissolved oxygen between 1990s and 2000s. Lowery 1998, Windsor 1985, Bricker et al. 1999, 2007 Florida Choctawhatchee River None 1980 Windsor 1985

Florida Clowers Creek Documented 1980 Windsor 1985 Florida Cross Bayou Canal Documented 1980 Windsor 1985

Florida Crystal Bay Concern 1980 Windsor 1985 Florida Crystal River Concern 1980 Windsor 1985 Florida Deadman Bay None 1980 Windsor 1985 Florida Deerpoint Lake Concern 1980 Windsor 1985 Florida Delaney Creek Documented 1990 Windsor 1985 Florida East Bay, Pensacola Documented 1980 Some improvement in dissolved oxygen between 1980s and 2000s. Improved from nutrient management, municipal and industrial. Windsor 1985

Florida East Bay, Apalachicola None 1980 Windsor 1985

Florida Econfina Creek None 1980 Windsor 1985 Florida Econfina River Documented 1970 Some hypoxia in 1970s, dissolved oxygen improved with effluent control. Winter and summer hypoxia. Windsor 1985, Livingston 1975

Florida Eleven Mile Creek Documented 1980 Windsor 1985

Florida Escambia Bay Documented 1960 Loss of fisheries species, fish kills. Summers et al. 1997, Windsor 1985

Florida Escambia River None 1980 Windsor 1985 Florida Everglades GAC Canal Documented 1970 Windsor 1985

Florida Fenholloway River Documented 1970 Hypoxia and anoxia in 1970 attributed to pulp mill effluents. Dissolved oxygen improved with effluent control. Winter and summer hypoxia and anoxia. Windsor 1985, Livingston 1975

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 121 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Florida Florida Bay Documented 2000 Some improvement in dissolved oxygen between 1990s and 2000s but hypoxic events may be more frequent in 2000s. Bricker et al. 1999, 2007, TIEE 2008

Florida Gordon River Documented 1980 Windsor 1985 Florida Hillsborough Bay Documented 1970 Almost complete mortality of benthos in late summer. Improved from nutrient management. Santos and Simon 1980, Windsor 1985, Bricker et al. 2007 Florida Hillsborough River Documented 1980 Windsor 1985

Florida Inner Continental Shelf Documented 2000 Mortality of benthos. Mortality of fishes from combined effects of HAB and low dissolved oxygen. Low dissolved oxygen from HAB (toxic dinoflagellate Karenia brevis ) in August 2005. Multiple stressors involved in mortalities. Landsberg 2009

Florida Jones and Jackson Creeks Documented 1980 Windsor 1985

Florida Lake Panasoffkee None 1980 Windsor 1985

Florida Lake Rousseau None 1980 Windsor 1985 Florida Little Aucilla River Documented 1980 Windsor 1985

Florida Long Bayou Documented 1980 Windsor 1985 Florida Looe Key Documented 1980 Mortality of benthos and corals. General eutrophication affecting water quality, dissolved oxygen conditions worsened from 1990s to 2000s. Rainfall events followed by low dissolved oxygen in seagrass and coral reef areas. Lapointe and Matzie 1996, Whitledge 1985, Bricker et al. 2007

Florida Martin Bayou Concern 1980 Windsor 1985 Florida Mulatto and Indian Bayous Documented 1980 Windsor 1985

Florida Munson Slough Documented 1980 Windsor 1985 Florida Myakka River Documented 1980 Combination of agricultural runoff and swamp drainage. Windsor 1985

Florida North Bay Concern 1980 Windsor 1985 Florida Ochlockonee Bay None 1980 Windsor 1985

Florida Ochlockonee River None 1980 Windsor 1985

Florida Old River Documented 1990 Part of regional water quality monitoring program. Engle et al. 1999

Florida Otter Creek None 1980 Windsor 1985 Florida Patch Reef Documented 1990 Mortality of benthos. Rainfall events followed by low dissolved oxygen in seagrass and coral reef areas. Lapointe and Matzie 1996

Florida Peace River Documented 1980 Hurricanes worsened hypoxia in 2004. Worse after hurricanes in 2004. Algal blooms from nutrients and sewage. Windsor 1985, Tomasko et al. 2006, Turner et al. 2006

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 122 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Florida Pensacola Bay Documented 1960 Loss of fisheries species, fish kills. Reduction in shrimp catch starting in 1960s attributed to multiple stressors including low dissolved oxygen. Some improvement in dissolved oxygen between 1980s and 2000s. Lowery 1998, Hagy et al. 2006, Windsor 1985, Hagy and Murrell 2007

Florida Perdido Bay Documented 1960 Dissolved oxygen poor in 1960s, but improved 1970s to 1980s. Dissolved oxygen declined from 1990s to 2000s. Stratification and industrial effluents highly significant factors, high nutrient loads. Flemer et al. 1999, Whitledge 1985, Bricker et al. 1999, 2007

Florida Perdido River Documented 1960 Windsor 1985 Florida Philippi Creek Documented 1980 Windsor 1985 Florida Pine Channel Documented 1990 Mortality of benthos. Rainfall events followed by low dissolved oxygen in seagrass and coral reef areas. Lapointe and Matzie 1996

Florida Port Pine Documented 1990 Mortality of benthos. Rainfall events followed by low dissolved oxygen in seagrass and coral reef areas. Lapointe and Matzie 1996

Florida Rocky Creek None 1980 Windsor 1985

Florida Rookery Bay Documented 1990 Dissolved oxygen levels declined from 1990s to 2000s. Rabalais 1998, Sanger et al. 2002, Bricker et al. 1999, 2007 Florida Santa Rosa Sound None 1980 Windsor 1985

Florida Sarasota Bay Documented 1980 SAV recovering. Dissolved oxygen levels improved from 1990s to 2000s. Windsor 1985, Bricker et al. 1999, 2007 Florida Sopchoppy River None 1980 Windsor 1985 Florida Spring Warrior Creek None 1980 Windsor 1985

Florida St. Andrew Bay Documented 1970 Hypoxia reduced from nutrient management, municipal and industrial. Windsor 1985

Florida St. George Sound Documented 1990 No dissolved oxygen problem in 1980s. Part of regional water quality monitoring program. Engle et al. 1999, Windsor 1985

Florida St. Joseph Bay Documented 1990 Avoidance by mobile fauna and brittlestar migration. Was not hypoxic in 1980s. Windsor 1985, Leonard and McClintock 1999 Florida St. Marks River None 1980 Windsor 1985 Florida Steinhatchee River None 1980 Windsor 1985

Florida Suwannee River Concern 1980 Windsor 1985

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 123 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Florida Tampa Bay Documented 1980 SAV recovering. Improved conditions for dissolved oxygen from 1980s to 1990s, dissolved oxygen worsened from 1990s to 2000s. Improved from nutrient management. Leverone 1995, Lowery 1998, Bricker et al. 1999, 2007

Florida Ten Mile Creek None 1980 Windsor 1985 Florida Ten Thousand Islands North Documented 2000 Bricker et al. 1999, 2007 Florida Ten Thousand Islands South Documented 2000 Bricker et al. 1999, 2007
Florida Turkey Creek None 1980 Windsor 1985 Florida Useppa Island Documented 1940 Some fish mortality. Low dissolved oxygen from HAB ( Gymnodinium ). Gunter et al. 1948

Florida Waccasassa Bay None 1980 Windsor 1985 Florida Wakulla River Concern 1980 Windsor 1985 Florida Watson Bayou Documented 1980 Part of regional water quality monitoring program. Windsor 1985, Engle et al. 1999 Florida Weeki Wachee Springs Documented 1980 Windsor 1985

Florida West Bay Concern 1980 Windsor 1985 Florida Whitaker Bayou Documented 1980 Windsor 1985 Florida Withlacoochee River Concern 1980 Windsor 1985

Florida Yellow River None 1980 Windsor 1985 Louisiana Amite River Documented 1990 Reduced benthos and loss of large clams. Penland et al. 2002 (http://pubs.usgs.gov/ of/2002/of02-206/ index.html) Louisiana Atchafalaya Bay Documented 2000 Not hypoxic in 1980s. Stratification highly significant factor. Rabalais et al. 1985, Bricker et al. 2007

Louisiana Atchafalaya River None 1980 Rabalais et al. 1985, Bricker et al. 2007 Louisiana Balize Delta Documented 1950 Low dissolved oxygen present in 1950s based on foraminifera proxies. Brunner et al. 2006

Louisiana Barataria Bay Documented 1950 Not hypoxic in 1980s. Rabalais et al. 1985, Bricker et al. 2007, Engle et al. 1999 Louisiana Blind Bay Concern 1990 Engle et al. 1999 Louisiana Breton Sound Documented 1990 Not hypoxic in 1980s. Rabalais et al. 1985, Bricker et al. 2007, Engle et al. 1999 Louisiana Caillou Bay None 1980 Rabalais et al. 1985 Louisiana Calcasieu Estuary Concern 1980 Whitledge 1985

Louisiana Calcasieu Lake Concern 1980 Some improvement in dissolved oxygen levels between 1990s and 2000s. Rabalais et al. 1985, Bricker et al. 1999, 2007

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 124 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Louisiana Calcasieu Pass/ Channel Concern 1980 Rabalais et al. 1985, Bricker et al. 2007
Louisiana Chandeleur Sound Documented 1990 Not hypoxic in 1980s. Rabalais et al. 1985, Bricker et al. 2007, Engle et al. 1999 Louisiana East Cote Blanche Bay None 1980 Rabalais et al. 1985

Louisiana Graden Island Bay Documented 1990 Part of regional water quality monitoring program. Engle et al. 1999

Louisiana Grand Bay Documented 1990 Part of regional water quality monitoring program. Engle et al. 1999

Louisiana Lake Boudreaux None 1980 Rabalais 1998 Louisiana Lake Cataouatche Documented 1970 Fish kills. Rabalais 1998

Louisiana Lake Maurepas None 1970 Rabalais 1998 Louisiana Lake Penchant Concern 1980 Low dissolved oxygen may have caused bass to leave the lake. Rabalais et al. 1985

Louisiana Lake Pontchartrain Documented 1970 Reduced species diversity. Loss of large clams. 250 km 2 USGS estimate. Abadie and Poirrier 2000, Rabalais et al. 1985, Penland et al. 2002 Louisiana Lake Tambour None 1980 Rabalais 1998 Louisiana Mermentau River None 1990 Bricker et al. 1997

Louisiana Mississippi/ Atchafalaya River Plumes Documented 1970 Avoidance by mobile fauna. Mortality of benthos with annual recolonization, Loss of biomass. Area coincides with historic white and brown shrimp fishing grounds. Annual hypoxia developed in 1970s, is now the largest hypoxic area in US. Major events in Mississippi watershed may have led to low dissolved oxygen prior to 1700s. Turner and Rabalais 1994, Justic et al. 1996, Sen Gupta et al. 1996, Rabalais et al. 2007, Rabalais and Turner 2001, Osterman et al. 2007, Craig and Crowder 2005 Louisiana Moss Bay None 1980 Rabalais 1998 Louisiana Terrebonne Bay Concern 1980 Rabalais et al. 1985 Louisiana Timbalier Bay None 1980 Rabalais et al. 1985 Louisiana Vermilion Bay Documented 2000 Not hypoxic in 1980s. Stratification highly significant factor. Rabalais et al. 1985, Bricker et al. 2007

Louisiana Wax Lake Documented 1990 Part of regional water quality monitoring program. Engle et al. 1999

Louisiana West Cote Blanche Bay None 1980 Rabalais et al. 1985

Louisiana West Hackberry Documented 1980 Mortality with annual recolonization. Associated with brine seep. Gaston 1985

Mississippi Back Bay Documented 1970 Low dissolved oxygen occurs but not a recurring problem in 1980s. Part of regional water quality monitoring program. Engle et al. 1999, Rabalais et al. 1985

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 125 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Mississippi Bayou Casotte/ Chico Documented 1970 Rabalais 1998

Mississippi Bayou La Batre Concern 1980 Rabalais 1998

Mississippi Big Lake Documented 1980 Episodic algal blooms. Rabalais et al. 1985, Rabalais 1998

Mississippi Biloxi Bay Concern 1970 Low dissolved oxygen occurs but not a recurring problem in 1980s. Engle et al. 1999, Rabalais et al. 1985

Mississippi Biloxi River Concern 1990 Rabalais 1998 Mississippi Escatawpa River Documented 1970 Rabalais et al. 1985 Mississippi Jourdan River Concern 1990 Rabalais 1998 Mississippi Lake Borgne Concern 1980 Rabalais et al. 1985 Mississippi Little River Concern 1980 Rabalais 1998 Mississippi Mississippi Inner Shelf, Deeper None 1950 Based on foraminifera proxies, there was no low dissolved oxygen in deeper areas >50 m. Brunner et al. 2006

Mississippi Mississippi Inner Shelf, Shallow Documented 1950 Sporadic low dissolved oxygen in 1800s associated with flood events, by 1940s low dissolved oxygen increased and intensified in 1960s based on foraminifera proxies. Rabalais et al. 1985, Brunner et al. 2006

Mississippi Mississippi River, Lower None 1980 Rabalais et al. 1985

Mississippi Mississippi Sound East Documented 1980 Dissolved oxygen improved from 1990s to 2000s. Stratification highly significant factor. Rabalais et al. 1985, Bricker et al. 1999, 2007

Mississippi Mississippi Sound West Documented 1950 Low dissolved oxygen in 1950s, intensified in 1960s based on foraminifera proxies. Rabalais et al. 1985, Bricker et al. 2007, Brunner et al. 2006
Mississippi Pascagoula Bay Concern 1980 Dissolved oxygen declined from 1980s to 1990s. Rabalais et al. 1985, Rabalais 1998 Mississippi Pascagoula River Documented 1970 Hypoxic from March to October. Rabalais 1998

Mississippi Pearl River None 1980 Rabalais et al. 1985, Rabalais 1998 Mississippi St. Louis Bay None 1980 Rabalais et al. 1985 Mississippi Tchoutacabouffa River Concern 1990 Rabalais 1998

Mississippi West Fowl River Concern 1980 Rabalais 1998 Mississippi Wolf River None 1990 Rabalais 1998
Texas Alazan Bay Concern 1980 Rabalais 1998

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 126 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Texas Aransas Bay Documented 2000 Diel hypoxia alters nursery value of SAV beds. Larval red drum, Sciaenops ocellatus , growth reduced when exposed to hypoxia >8 hours. Open Bay had little low dissolved oxygen in 1980s. Increased nutrient loads lower dissolved oxygen in SAV beds. Lowery 1998, Rabalais et al. 1985, Perez-Domingues et al. 2006

Texas Arroyo Colorado Documented 1980 Hypoxia or anoxia occur yearround. Engle et al. 1999, Rabalais et al. 1985 Texas Baffin Bay Documented 1980 Rabalais et al. 1985, Bricker et al. 2007 Texas Brazos River Documented 1980 Rabalais et al. 1985, Bricker et al. 2007 Texas Brazos Santiago Pass None 1980 Rabalais 1998

Texas Brownsville Ship Channel Documented 1980 Rabalais 1998

Texas Bryan Mound, deep Documented 1980 Decreased diversity and abundance of mobile species at the onset of hypoxia. Mortality of benthos with annual recolonization. Harper et al. 1981, 1991

Texas Bryan Mound, shallow Documented 1970 Stressed fishes, mass mortality of benthos with multi-year
recovery. Harper et al. 1981, 1991

Texas Cayo del Grullo Concern 1980 Rabalais 1998
Texas Cedar Bayou Documented 1980 Rabalais et al. 1985
Texas Chocolate Bay Documented 1990 Part of regional water quality monitoring program. Engle et al. 1999

Texas Colorado River None 1980 Rabalais et al. 1985
Texas Copano Bay None 1980 Rabalais 1998
Texas Corpus Christi Bay Documented 1980 Reduced, biomass at hypoxic stations is 96% less than at stations with normal oxygen levels. Hypoxia expanded in the 1990s. Low bottom dissolved oxygen when bottom salinity was high. Ritter and Montagna 1999, Montagna and Ritter 2006, Montagna and Kalke 1992, Applebaum et al. 2005 Texas Cox Bay None 1980 Rabalais 1998
Texas Dickinson Bayou Documented 1980 Rabalais et al. 1985
Texas East Bay Documented 1980 Whitledge 1985
Texas Espiritu Santo Bay None 1980 Rabalais 1998

Texas Freeport Documented 1970 Avoidance, some mortality of fishes. Mortality of benthos with multi-year
recovery. Harper and Rabalais 1995

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 127 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Texas Freeport Ship Channel None 1980 Rabalais et al. 1985

Texas Galveston Bay Concern 1970 Lowery 1998, Rabalais et al. 1985 Texas Guadalupe Estuary None 1980 Lowest frequency of low dissolved oxygen of all Texas estuaries in 1980s. Rabalais et al. 1985, Rabalais 1998

Texas Keller Bay None 1980 Rabalais 1998 Texas Laguna Madre, Lower Documented 1980 Sewage and shrimp farming, most low dissolved oxygen in deeper areas, diel low dissolved oxygen in shallow

areas. Ziegler and Benner 1998, Rabalais et al. 1985, Bricker et al. 2007

Texas Laguna Madre, Upper Documented 1980 Stratification highly significant factor, most low dissolved oxygen in deeper areas. Rabalais et al. 1985, Bricker et al. 2007

Texas Laguna Salada Concern 1980 Rabalais 1998
Texas Lavaca Bay Documented 1990 Part of regional water quality monitoring program. Engle et al. 1999

Texas Matagorda Bay Documented 1980 Lowery 1998, Rabalais et al. 1985, Bricker et al. 2007 Texas Matagorda Ship Channel Documented 1980 Rabalais 1998

Texas Mesquite Bay None 1980 Rabalais 1998
Texas Mission Bay None 1980 Rabalais 1998
Texas Mission-Aransas Estuary Documented 1980 Rabalais et al. 1985, Rabalais 1998

Texas Nueces Bay Documented 1980 Rabalais 1998
Texas Nueces Estuary Concern 1980 Rabalais 1998
Texas Offatts Bayou Documented 1930 Mass mortality of benthos and fishes. First occurrence 1936. Part of regional water quality monitoring program. Gunter 1942, Engle et al. 1999, White et al. 1984 Texas Oso Bay Concern 1980 Rabalais 1998
Texas Port Arthur Canal Concern 1980 Rabalais et al. 1985

Texas Redfish Bay None 1980 Rabalais 1998
Texas Rio Grand Tidal Concern 1980 Rabalais et al. 1985
Texas Sabine Lake Concern 1980 Rabalais et al. 1985 Texas Sabine Pass Concern 1980 Rabalais et al. 1985
Texas Sabine-Neches Estuary Documented 1980 Rabalais et al. 1985

Texas San Antonio Bay Documented 1980 Dissolved oxygen declined from 1980s to 1990s. Stratification highly significant factor. Lowery 1998, Rabalais et al. 1985

Texas San Antonio River None 1980 Rabalais 1998

Texas San Bernard River Documented 1990 Part of regional water quality monitoring program. Engle et al. 1999

Texas South Bay None 1980 Rabalais 1998

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 128 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Texas Texas Inner Shelf Concern 1980 Rabalais et al. 1985

Texas Tres Palacios Bay None 1980 Rabalais 1998

Texas Trinity Bay Concern 1980 Whitledge 1985
Texas Trinity-San Jacinto Estuary Concern 1990 Rabalais 1998

Texas Upper Galveston Bay Channel Documented 1980 Part of regional water quality monitoring program. Engle et al. 1999, Rabalais et al. 1985

Texas West Bay Concern 1980 Rabalais et al. 1985 

### Mid-Atlantic Region

Connecticut Ash Creek Documented 1980 Whitledge 1985 Connecticut Black Rock Harbor None 1980 Whitledge 1985

Connecticut Bridgeport Harbor None 1980 Whitledge 1985

Connecticut Burr Creek Documented 1980 Whitledge 1985 Connecticut Connecticut River Documented 1980 Episodic events in tidal freshwater zone. Whitledge 1985, Bricker et al. 2007 Connecticut Housatonic River None 1980 Whitledge 1985 Connecticut Johnson Creek Concern 1980 Whitledge 1985 Connecticut Naugatuck River Concern 1980 Whitledge 1985 Connecticut Pawcatuck River Concern 1980 Whitledge 1985 Connecticut Quinnipiac River Concern 1980 Whitledge 1985 Connecticut Thames River None 1980 Whitledge 1985 Connecticut Yellow Mill Channel Concern 1980 Whitledge 1985

Delaware Bald Eagle Creek Documented 2000 Fish kills. Eutrophication and limited tidal exchange with adjacent body of water. Luther et al. 2004

Delaware Blackwater Landing Documented 1990 Sanger et al. 2002

Delaware Dead-end canals/ Inland bays Documented 1990 Dead-end canals have limited tidal exchange with adjacent body of water. Maxted et al. 1997

Delaware Delaware Bay None 1980 Whitledge 1985 Delaware Delaware River Documented 1910 Recovering American shad and striped bass fishery. Low dissolved oxygen first recorded in 1915. Recovered from low dissolved oxygen through nutrient management. Weisberg et al. 1996, Summers et al. 1997, Patrick 1988

Delaware Indian River Bay Documented 1980 Whitledge 1985 Delaware Little Assawoman Bay Concern 1980 Fish kills. Whitledge 1985, Bricker et al. 2007 Delaware Murderkill River Documented 1960 Caused by sewage discharge. deWitt and Daiber 1974 Delaware Pepper Creek Documented 2000 Fish migrate in and out of creek depending on dissolved oxygen levels. Daily cycle of dissolved oxygen from supersaturated to hypoxic. Tyler 2004, Tyler and Targett 2007

Delaware Rehoboth Bay Documented 1980 Whitledge 1985 Maryland Anacostia River Concern 1980 Whitledge 1985

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 129 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Maryland Assawoman Bay Documented 1980 No hypoxia in 1970s. Whitledge 1985, Bricker et al. 2007, Boynton et al. 1996 Maryland Back Creek None 1980 Whitledge 1985 Maryland Back River Documented 1980 Whitledge 1985 Maryland Baltimore Harbor Documented 1980 Whitledge 1985

Maryland Big Annemessex River None 1980 Whitledge 1985, CBP 2008 Maryland Bird River None 1980 Whitledge 1985

Maryland Bodkin Creek Documented 1980 Whitledge 1985 Maryland Bohemia None 1990 CBP 2008 Maryland Bush River None 1980 Whitledge 1985

Maryland Cabin John Creek Concern 1980 Whitledge 1985

Maryland Chesapeake Bay Mainstem Documented 1930 Low dissolved oxygen killed crabs in crab pots. Mortality of benthos with annual recolonization. Seasonal anoxia detected in sediment record as far back as 1934-1948. Low dissolved oxygen events in the 1800s. Low dissolved oxygen occurrence has increased from 1980 to 2000, area of hypoxia and anoxia has increased. Zimmerman and Canual 2000, Newcombe and Horne 1938, Officer et al. 1984, Seliger et al. 1985, Holland et al., 1987, Boesch et al. 2001, Hagy et al. 2004, Kemp et al. 2005 Maryland Chester River Documented 1980 Avoidance and some mortality of fishes. Mortality of benthos with annual recolonization. Low dissolved oxygen events occur in June-Aug. Dissolved oxygen declined from 1990s to 2000s Whitledge 1985, Bricker et al. 1999, 2007. CBP 2008

Maryland Chincoteague Bay Concern 1980 No hypoxia in 1970s. Whitledge 1985, Bricker et al. 2007, Boynton et al. 1996 Maryland Choptank River Documented 1980 Low dissolved oxygen events occur in June-Sept. Whitledge 1985, Bricker et al. 2007

Maryland Corsica River Documented 2000 Fish kill from combined stress of HAB toxins and low dissolved oxygen. Bricker et al. 2007

Maryland Dead-end canals Documented 1990 Dead-end canals have limited tidal exchange with adjacent body of water. Maxted et al. 1997

Maryland Eastern Bay None 1990 CBP 2008 Maryland Elk River None 1980 Whitledge 1985 Maryland Fishing Bay None 1990 CBP 2008

Maryland Gunpowder River None 1980 Whitledge 1985, CBP 2008

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 130 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Maryland Honga None 1990 CBP 2008

Maryland Isle of Wight Bay Documented 1980 No hypoxia in 1970s. Whitledge 1985, Bricker et al. 2007, Boynton et al. 1996 Maryland Jug Bay Documented 1990 Sanger et al. 2002 Maryland Little Choptank None 1990 CBP 2008 Maryland Little River/ Browns Creek None 1980 Whitledge 1985

Maryland Magothy Documented 1990 CBP 2008 Maryland Manokin None 1990 CBP 2008

Maryland Manokin River Concern 1980 Whitledge 1985 Maryland Mattawoman Creek None 1980 Whitledge 1985, CBP 2008 Maryland Middle None 1990 CBP 2008 Maryland Miles River None 1980 Whitledge 1985 Maryland Nanjemoy Creek None 1980 Whitledge 1985 Maryland Nanticoke River None 1980 Whitledge 1985 Maryland Nassawango Creek None 1980 Whitledge 1985

Maryland Newport Bay None 1980 No hypoxia in 1970s. Whitledge 1985, Bricker et al. 2007, Boynton et al. 1996 Maryland Northeast River None 1980 Whitledge 1985 Maryland Oxon Run Concern 1980 Whitledge 1985 Maryland Patapsco River Documented 1990 Riedel et al. 1999, CBP 2008 Maryland Patuxent River Documented 1930 Avoidance, low egg hatching/ larval mortality, bay anchovy eggs killed by hypoxia. Mortality of benthos with annual recolonization. Duration and spatial extent of low dissolved oxygen has increased from 1930-79. Dissolved oxygen declined from 1990s to 2000s. Indications that hypoxia occurred in 1700s from watershed modifications and wet periods. Rise in anoxia in 1970s linked to nutrient runoff and wet weather. Keister et al. 2000, Breitburg et al. 1997, 1997, Bricker et al. 1999, 2007, Cronin and Vann 2003

Maryland Piscataway Creek Concern 1980 Whitledge 1985

Maryland Pocomoke River None 1980 Whitledge 1985 Maryland Port Tobacco River Concern 1980 Whitledge 1985

Maryland Potomac River Documented 1910 Mortality of benthos with annual recolonization. Low dissolved oxygen in May-Sept; increased spatial coverage of hypoxia from 1910s. Some improvement in dissolved oxygen levels from 1990s to 2000s. Sale and Skinner 1917, Whitledge 1985, Bricker et al. 1999, 2007, CBP 2008

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 131 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Maryland Rhode River None 1990 CBP 2008 Maryland Rock Creek Concern 1980 Whitledge 1985 Maryland Sassafras River None 1980 Whitledge 1985 Maryland Seneca Creek Concern 1980 Whitledge 1985 Maryland Severn River Documented 1990 CBP 2008
Maryland Sinepuxent Bay Concern 1980 No hypoxia in 1970s. Whitledge 1985, Bricker et al. 2007, Boynton et al. 1996 Maryland St. Leonard Creek Documented 1990 Summers et al. 1997

Maryland St. Martin River None 1970 No hypoxia in 1970s. Boynton et al. 1996 Maryland St. Marys River Concern 1980 Whitledge 1985 Maryland Susquehanna River Concern 1980 Whitledge 1985

Maryland Swan Creek None 1980 Whitledge 1985

Maryland Tangier Sound Concern 1990 Rabalais 1998, Bricker et al. 2007 Maryland Turville Creek None 1970 No hypoxia in 1970s. Boynton et al. 1996 Maryland Wicomico River None 1990 CBP 2008 Maryland Wye River None 1980 Whitledge 1985 Massachusetts Acushnet River Concern 1970 Fish kill, maybe dissolved oxygen related August 1973. Whitledge 1985

Massachusetts Apponaganset Bay None 1970 Whitledge 1985

Massachusetts Buzzards Bay Documented 1980 Summer hypoxia. Whitledge 1985, Bricker et al. 2007, Buzzards Bay NEP 2008 Massachusetts Clarks Cove None 1970 Whitledge 1985 Massachusetts New Bedford Harbor Documented 1930 Loss of fishes and shellfish. Low dissolved oxygen may have occurred in 1800s associated with whaling and textile industries, by 1930 water quality poor. Whitledge 1985, NOAA 2008

Massachusetts Tauton River Documented 1970 Whitledge 1985 New Jersey Barnegat Bay Documented 1980 Mass mortality of benthos with annual recolonization. Whitledge 1985, Moser 1998

New Jersey Great Egg Harbor River Documented 1980 Whitledge 1985, Glenn et al. 1996 New Jersey Inland Bays Concern 2000 Bricker et al. 2007 New Jersey Little Egg Inlet Documented 1970 Garlo et al. 1979 New Jersey Metedeconk River None 1980 Whitledge 1985

New Jersey Mullica River Estuary Documented 1990 Dissolved oxygen low in 1980s, hypoxic in 1990s. Whitledge 1985, Glenn et al. 1996 New Jersey Newark Bay Concern 1980 Whitledge 1985 New Jersey Passaic River Concern 1980 Whitledge 1985

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 132 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

New Jersey Raritan Bay Documented 1970 Some improvement in dissolved oxygen from 1990s to 2000s. Improved from nutrient management. Christensen and Packard 1976, Whitledge 1985, Bricker et al. 1999, 2007 New Jersey Raritan River Documented 1970 Whitledge 1985 New Jersey Sandy Hook Bay None 1980 Whitledge 1985 New Jersey Toms River None 1980 Whitledge 1985 New Jersey TownsendHereford Inlet Documented 1990 Glenn et al. 1996, 2004 New Jersey Tuckahoe River None 1980 Whitledge 1985 New Jersey Wading River Concern 1980 Whitledge 1985 New York Arthur Kill Documented 1970 Whitledge 1985 New York Berger Basin Documented 1970 Rhoads et al. 2001 New York Carmans River Documented 1990 Diel hypoxia present at times. Zaikowski et al. 2008 New York East River Documented 1920 Decline in dissolved oxygen recorded in 1910-1930. Now dissolved oxygen improved from nutrient management. Parker and O'Reilly 1991, Whitledge 1985

New York Flushing Bay Documented 1990 Rhoads et al. 2001 New York Gardiners Bay None 1980 Whitledge 1985 New York Grassy Bay Documented 1970 Areas anoxic in June-August. Rhoads et al. 2001, Bricker et al. 2007
New York Great South Bay Documented 1980 Improved, but tidal creeks periodically hypoxic between July and September. Diel hypoxia may occur. Whitledge 1985, Bricker et al. 2007, Zaikowski et al. 2008

New York Hackensack Concern 1980 Whitledge 1985 New York Harlem River Documented 1970 Whitledge 1985 New York Hudson River Documented 1960 Recovered from low dissolved oxygen through nutrient management. Bronsnan and O'Shea 1996, Whitledge 1985
New York Kill van Kull River Documented 1970 Whitledge 1985

New York Long Island Sound Documented 1970 Avoidance and some mortality of fishes. About 30% of Sound benthos impacted by hypoxia. Dissolved oxygen declined from 1990s to 2000s. Area of hypoxia has increased from 1980s. Hypoxic June to September; duration, frequency, spatial coverages all increased since 1987. Howell and Simpson 1994, Welsh et al. 1994, Schimmel et al. 1999, Anderson and Taylor 2001, Lee and Lwiza 2007

New York Lower Bay Concern 1980 Whitledge 1985 New York Mill Basin Documented 1990 Avoidance by mobile fauna. Rhoads et al. 2001

New York Mohawk River Concern 1980 Whitledge 1985 New York Narrows Documented 1970 Whitledge 1985, Lee and Lwiza 2007

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 133 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

New York New York Bight Documented 1970 Surf clam/ finfish mortality, Avoidance, northward migration of bluefish blocked. Mass mortality of benthos with multi-year recovery. Large economic losses. Calm weather led to stratification and bloom of Ceratium tripos , one time event. May have involved upwelling on nutrient-rich
water. Segar and Berberian 1976, Garlo et al. 1979, Sindermann and Swanson 1980, Swanson and Parker 1988, Figley et al. 1979, Azarovitz et al. 1979, Boesch and Rabalais 1991

New York New York Bight Sewage Dump Documented 1970 Summer hypoxia associated with sewage dump site, 10% saturation at center of dump site. Segar and Berberian 1976

New York New York City Harbor Documented 1920 Stressed fishes. Mass mortality of benthos with annual recolonization. Decline in dissolved oxygen recorded in 1910-1930. Now dissolved oxygen improved from nutrient management. Parker and O'Reilly 1991

New York Norton Basin Documented 1990 Avoidance by mobile fauna. Rhoads et al. 2001

New York Patchogue River Documented 1990 Benthic species richness severely impacted. Channelization induced stratification an important factor. Deep areas hypoxic year round. Zaikowski et al. 2008

New York Shellbank Basin Documented 1990 Rhoads et al. 2001 New York Swan River Documented 1990 Benthic species richness moderately to severely impacted. Channelization induced stratification an important factor. Zaikowski et al. 2008

New York Tivolie South Documented 1990 Sanger et al. 2002 New York Upper Bay Concern 1980 Whitledge 1985 Rhode Island Greenwich Bay Documented 2000 Fish kill in 2003. 0-4% of area <2.3 mg/l dissolved oxygen. Deacutis et al. 2006, Melrose et al. 2007 Rhode Island Mount Hope Bay Documented 2000 0.5-2.5% of area <2.3 mg/l dissolved oxygen. Deacutis et al. 2006, Melrose et al. 2007 Rhode Island Narragansett Bay Documented 1950 Reduced abundance or absence of benthos. Hypoxic June to September, related to high nutrient loads. Hypoxia and anoxia present in 1950s in upper part of bay, low dissolved oxygen widespread by 1980s. Dissolved oxygen declined from 1990s to 2000s. Whitledge 1985, Bricker et al. 1999, 2007, Melrose et al. 2007, Bergondo et al. 2005, Deacutis et al. 2006, Cicchetti et al. 2006 Rhode Island Pettaquamscutt River Documented 1990 Wilkin and Barnes 1997 Rhode Island Potters Cove Documented 1990 Sanger et al. 2002 Rhode Island Providence River Documented 1980 Related to sewage discharges, 27-39% of the river was <2.3 mg/l dissolved oxygen. Whitledge 1985, Bergondo et al. 2005, Deacutis et al. 2006, Melrose et al. 2007 Virginia Appomattox River None 1990 CBP 2008

Virginia Back Bay None 1980 Whitledge 1985

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 134 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Virginia Chesapeake Bay, lower Documented 1980 Lower benthic diversity, lower biomass, lowerproportion of deepdwelling biomass at hypoxia-affected

areas. Dauer et al 1992

Virginia Chickahominy None 1990 CBP 2008 Virginia Chuckatuck River None 1990 CBP 2008

Virginia Cobb Bay None 1980 Whitledge 1985 Virginia Corrotoman River None 1990 CBP 2008

Virginia East River None 1990 CBP 2008 Virginia Elizabeth River, Eastern Branch Documented 1990 CBP 2008

Virginia Elizabeth River, Southern Branch Documented 1990 CBP 2008

Virginia Elizabeth River, Western Branch Concern 1990 CBP 2008

Virginia Goodwin Island Documented 1990 Low dissolved oxygen in submerged aquatic vegetation bed. Sanger et al. 2002

Virginia Grays Creek None 1990 CBP 2008 Virginia Hog Island Bay None 1980 Whitledge 1985 Virginia James River None 1980 Dissolved oxygen improved from nutrient management. Whitledge 1985

Virginia Jones-Gilligan Creek None 1990 CBP 2008

Virginia Lafayette River Concern 1990 CBP 2008 Virginia Lynnhaven Inlet None 1990 CBP 2008 Virginia Mattaponi River Documented 1990 CBP 2008 Virginia Mobjack Bay Documented 1990 CBP 2008 Virginia Mud Creek None 1990 CBP 2008 Virginia Nansemond River Documented 1990 CBP 2008

Virginia North River None 1990 CBP 2008 Virginia Onancock Creek Documented 2000 Wang 2005 Virginia Pagan River None 1990 CBP 2008 Virginia Pamunkey River None 1990 CBP 2008 Virginia Paradise Creek None 1990 CBP 2008 Virginia Piankatank River None 1990 CBP 2008 Virginia Pocomoke Sound None 1980 Whitledge 1985 Virginia Poropotank River None 1990 CBP 2008

Virginia Queen Creek None 1990 CBP 2008 Virginia Rappahannock River Documented 1980 Avoidance by mobile fauna. Mortality of benthos with annual recolonization. Some anoxia in summer. Llansó 1992, Kuo et al. 1991

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 135 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Virginia Robinson Creek None 1990 CBP 2008 Virginia Sarah Creek None 1990 CBP 2008 Virginia Scuffletown Creek None 1990 CBP 2008

Virginia Severn Creek None 1990 CBP 2008 Virginia South Bay None 1980 Whitledge 1985 Virginia Taskinas Creek Documented 1990 Sanger et al. 2002 Virginia Timberneck Creek None 1990 CBP 2008

Virginia Totuskey Creek None 1990 CBP 2008 Virginia Urbanna Creek None 1990 CBP 2008 Virginia Ware River None 1990 CBP 2008 Virginia Warwick River None 1990 CBP 2008 Virginia Weeks Creek None 1990 CBP 2008 Virginia Willoughby Bay None 1990 CBP 2008 Virginia York River Documented 1970 Avoidance by mobile fauna with reduced diversity following low dissolved oxygen events. Little to no response in the benthos. Hypoxia associated with spring-neap tidal cycle. Haas 1977, Pihl et al. 1991, 1992, Diaz et al. 1992, Sagasti et al 2001, 2003, Seitz et al. 2003



### Northeast Region

Maine Androscoggin River None 1980 Whitledge 1985, Bricker et al. 2007 Maine Blue Hill Bay None 1980 Whitledge 1985 Maine Casco Bay None 1980 Whitledge 1985, Bricker et al. 2007 Maine Cobscook Bay None 1980 Whitledge 1985 Maine Damariscotta River None 1980 Whitledge 1985

Maine Englishman Bay None 1980 Whitledge 1985 Maine Fore River None 1980 Whitledge 1985 Maine Frenchman Bay None 1980 Whitledge 1985 Maine Great Bay Concern 2000 Evidence of decline in dissolved oxygen levels. Bricker et al. 2006

Maine Kennebec River None 1980 Whitledge 1985 Maine Machias Bay None 1980 Whitledge 1985 Maine Machias River Estuary None 1980 Whitledge 1985

Maine Muscongus Bay None 1980 Whitledge 1985 Maine Narraguagus Bay None 1980 Whitledge 1985 Maine Narraguagus River Concern 1980 Whitledge 1985

Maine Passamaquoddy Bay None 1980 Whitledge 1985

Maine Penobscot Bay/ River None 1980 Whitledge 1985

Maine Pleasant Bay None 1980 Whitledge 1985 Maine Saco Bay None 1980 Whitledge 1985

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 136 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Maine Sheepscot Bay None 1980 Whitledge 1985 Maine St. Croix River None 1980 Whitledge 1985 Maine Wells Bay Documented 1990 Sanger et al. 2002 Maine Wells Inlet Documented 1990 Daily cycle of dissolved oxygen from supersaturated to hypoxic, Submerged aquatic vegetation bed. Sanger et al. 2002

Maine Wohoa Bay None 1980 Whitledge 1985

Massachusetts Boston Harbor None 1980 Entire region recovered from low dissolved oxygen through nutrient management. Whitledge 1985, Bricker et al. 1999, 2007, Diaz et al. 2008 Massachusetts Cape Cod Bay Documented 1990 Blooms. Whitledge 1985, Rabalais 1998
Massachusetts Charles River Documented 1980 Taylor 2000

Massachusetts Dorchester Bay Documented 1980 Had poor water quality in 1980s. Low dissolved oxygen in 1980s now improved from nutrient management. Maciolek et al. 2005, Diaz et al. 2008

Massachusetts Duxbury Bay None 1980 Whitledge 1985 Massachusetts Fore River Documented 1980 Neponset River Watershed Association 2004 Massachusetts Herring River Documented 1980 Fish kills, decline of alewife fishery. Anoxia, 1-3 weeks. Portnoy 1991

Massachusetts Massachusetts Bay None 1990 Bricker et al. 2007

Massachusetts Merrimack River Documented 1970 Whitledge 1985 Massachusetts Plum Island Sound None 1990 Rabalais 1998, Bricker et al. 2007
Massachusetts Waquoit Bay Documented 1990 Daily cycle of dissolved oxygen from saturated to hypoxic. Fritz et al. 1996, D'Avanzo and Kremer 1994, Bricker et al. 2007 New Hampshire Great Bay Documented 1980 Whitledge 1985, Bricker et al. 2007

New Hampshire Hampton Harbor Estuary None 1990 Rabalais 1998

New Hampshire Piscataqua River None 1980 Whitledge 1985, Bricker et al. 2007 

### South Atlantic Region

Florida Amelia River Documented 1970 Windsor 1985 Florida Banana River Concern 1980 Windsor 1985

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 137 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Florida Biscayne Bay Documented 1970 Related to releases of freshwater from canals. Dissolved oxygen declined from 1990s to 2000s. Bricker et al. 1999, 2007, Windsor 1985, Leverone 1995

Florida Canal 17 Documented 1980 Whitledge 1985 Florida Cedar Creek Documented 1980 Windsor 1985 Florida East Holloway Canal Documented 1980 Windsor 1985

Florida Eau Gallie Harbor Concern 1980 Whitledge 1985

Florida Gulf and West Canals Documented 1980 Windsor 1985

Florida Halifax River Documented 1980 Windsor 1986 Florida Haw Creek Documented 1980 Windsor 1985 Florida Hillsboro Canal Documented 1980 Windsor 1985 Florida Indian River Documented 2000 Improvement in dissolved oxygen levels from 1990s to 2000s. Bricker et al. 1999, 2007, Windsor 1985

Florida Lofton Creek Concern 1980 Windsor 1985 Florida Loxahatchee River Concern 1980 Windsor 1985

Florida Matanzas River Concern 1980 Windsor 1985 Florida Miami Canal Documented 1980 Windsor 1985 Florida Mosquito Lagoon Concern 1980 Windsor 1985

Florida Naples Bay Documented 1980 Windsor 1985 Florida Nassau River None 1980 Windsor 1985 Florida North and South New River Canals Documented 1980 Windsor 1985

Florida North River Documented 1980 Windsor 1987 Florida Ortega River Documented 1980 Windsor 1985 Florida Pottsburg Creek Documented 1980 Windsor 1985 Florida Ribault River Documented 1980 Windsor 1985 Florida Rice Creek Documented 1980 Windsor 1985 Florida Sebastian Creek Documented 1980 Windsor 1985 Florida Sisters Creek Concern 1980 Windsor 1985 Florida South Amelia River Concern 1980 Windsor 1985

Florida South Relief Canal Documented 1980 Windsor 1985

Florida Spruce Creek None 1980 Windsor 1985 Florida St. Johns River Documented 1970 Mortality of benthos, massive fish kills with unknown cause, may be multiple
stressors. Windsor 1985, Mason 1998

Florida St. Lucie Canal Concern 1980 Windsor 1985

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 138 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Florida St. Lucie River Documented 1980 Chamberlain and Hayward 1996, Windsor 1985 Florida St. Marys River Documented 1970 Anoxia develops in

summer. No significant declining trend in dissolved oxygen from 1960s to 2000s Windsor 1985, Verity et al. 2006

Florida Sykes Creek Documented 1980 Windsor 1985 Florida Tenmile Creek Documented 1980 Windsor 1985 Florida Tolomato River Concern 1980 Windsor 1985 Florida Tomoka River Concern 1980 Windsor 1985 Florida Trout River Documented 1980 Windsor 1985 Florida Turkey Creek Documented 1980 Windsor 1986 Florida West Palm Beach Canal Documented 1980 Windsor 1985

Florida Whitewater Bay Concern 1980 Windsor 1985 Georgia Altamaha Sound Concern 2000 Declining trend in dissolved oxygen related to nutrient loadings. No hypoxia in 1980s. Stanley 1985, Verity et al. 2006

Georgia Altamaha River Documented 2000 Significant declining trend in dissolved oxygen from 1970s to 2000s. No hypoxia in 1980s. Stanley 1985, Verity et al. 2006

Georgia Brunswich River Concern 1970 Stanley 1985 Georgia Brunswick Harbor Concern 1970 Stanley 1985

Georgia Cumberland Sound Documented 2000 Declining trend in Dissolved oxygen related to nutrient loadings. Stanley 1985, Verity et al. 2006

Georgia Doboy Sound None 1980 Stanley 1985 Georgia Duplin River Documented 1970 Daily cycle of dissolved oxygen from supersaturated to hypoxic. Frankenberg 1976, Stanley 1985

Georgia Medway River None 1970 Stanley 1985 Georgia Ogeechee River Documented 2000 Significant declining trend in dissolved oxygen from 1960s to 2000s. No hypoxia in 1970s. Stanley 1985, Verity et al. 2006

Georgia Ogeechee Sound Concern 2000 Declining trend in dissolved oxygen related to nutrient loadings. Stanley 1985, Verity et al. 2006

Georgia Ossabaw Sound Documented 1980 Related to organic matter washing out of swamps and marshes. Dissolved oxygen declined from 1990s to 2000s. Stanley 1985, Bricker et al. 1999, 2007

Georgia Sapelo Island Documented 2000 Daily cycle of dissolved oxygen from supersaturated to hypoxic. Sanger et al. 2002

Georgia Sapelo Sound Concern 1990 No hypoxia in 1980s, declining trend is dissolved oxygen for 2000s. Stanley 1985, Verity et al. 2006

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 139 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Georgia Satilla River Documented 2000 Significant declining trend in dissolved oxygen from 1960s to 1990s. Not hypoxic in 1970s. Stanley 1985, Verity et al. 2006

Georgia Savannah River Documented 1980 Nonpoint sources a factor. Significant declining trend in dissolved oxygen from 1960s to 2000s. Stanley 1985, Verity et al. 2006, Bricker et al. 2007

Georgia Skidaway River Estuary Concern 1990 High rate of microbial respiration causes low dissolved oxygen despite strong vertical and horizontal mixing. Declining trend in dissolved oxygen related to nutrient loadings. Verity et al. 2006, Bricker et al. 2007

Georgia St. Andrews Sound Concern 1990 No hypoxia in 1980s, declining trend is dissolved oxygen for 2000s. Stanley 1985, Verity et al. 2006

Georgia St. Catherine Sound None 1980 Stanley 1985

Georgia St. Marys River Concern 1970 Stanley 1985 Georgia St. Simons Sound None 1980 Stanley 1985, Rabalais 1998, Bricker et al. 2007 Georgia Turtle River Documented 1970 Stanley 1985 Georgia Tybee Creek None 1970 Stanley 1985 Georgia Wassaw Sound None 1980 Stanley 1985 Georgia Wilmington River None 1970 Stanley 1985

North Carolina Ace Basin Documented 1990 Sanger et al. 2002 North Carolina Albemarle Sound Documented 1970 Mortality of benthos and mobile fauna. Hurricane increased freshwater and nutrient input. Hypoxia has worsened in 2000s. Stanley 1985, Bricker et al. 1999, 2007, Paerl et al. 2000 North Carolina Alligator River None 1980 Stanley 1985 North Carolina Barnards Creek Documented 2000 Combination of agricultural, urban, suburban runoff contribute to nutrient loading. Mallin et al. 2006

North Carolina Black River Documented 1990 Combination of agricultural, urban, suburban runoff contribute to nutrient loading. Mallin et al. 2006

North Carolina Bogue Sound Concern 1980 Stanley 1985 North Carolina Browns Creek Concern 2000 Combination of agricultural, urban, suburban runoff contribute to nutrient loading. Mallin et al. 2006

North Carolina Cape Fear River Documented 1970 Fish kills, reduced benthos. Stanley 1985, Mallin et al. 1999, 2006, Posey et al. 1999 North Carolina Carolina Beach Lake Documented 2000 Combination of agricultural, urban, suburban runoff contribute to nutrient loading. Mallin et al. 2006

North Carolina Chowan River Documented 1980 Stanley 1985

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 140 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

North Carolina Colly Creek Concern 2000 Combination of agricultural, urban, suburban runoff contribute to nutrient loading. Mallin et al. 2006

North Carolina Core Sound None 1980 Stanley 1985 North Carolina Currituck Sound None 1980 Stanley 1985 North Carolina Dutchman Creek Concern 1970 Stanley 1985 North Carolina Futch Creek Documented 2000 Combination of agricultural, urban, suburban runoff contribute to nutrient loading. MacPherson et al. 2007, Mallin et al. 2006 North Carolina Great Coharie Creek Documented 2000 Combination of agricultural, urban, suburban runoff contribute to nutrient loading. Mallin et al. 2006

North Carolina Hammond Creek Documented 2000 Combination of agricultural, urban, suburban runoff contribute to nutrient loading. Mallin et al. 2006

North Carolina Hewletts Creek Documented 2000 Heavily developed watershed. MacPherson et al. 2007, Mallin et al. 2006 North Carolina Little Coharie Creek Concern 2000 Combination of agricultural, urban, suburban runoff contribute to nutrient loading. Mallin et al. 2006

North Carolina Little River None 1980 Stanley 1985

North Carolina Masonboro Inlet Documented 1990 Sanger et al. 2002 North Carolina Motts Creek Documented 2000 Combination of agricultural, urban, suburban runoff contribute to nutrient loading. Mallin et al. 2006

North Carolina Neuse River Estuary Documented 1980 Fish kills, mortality of oyster. Reduced prey abundance and habitat quality for fish. Mortality of benthos with decreased abundance. Hypoxia may have developed earlier based on Macoma populations. Improvement in dissolved oxygen from 1990s to 2000s. Paerl et al. 1995, 1998, Lenihan and Peterson 1998, Lenihan 1999, Whitledge 1985, Buzzelli et al. 2002, Bishiop et al. 2006, Bell and Eggleston 2005, Baird et al. 2004, Aumann et al. 2006, Powers et al. 2005 North Carolina New River Documented 1980 Stanley 1985, Bricker et al. 2007 North Carolina Newport River None 1980 Stanley 1985 North Carolina North River None 1980 Stanley 1985 North Carolina Northeast Cape Fear River Documented 1990 Combination of agricultural, urban, suburban runoff contribute to nutrient loading. Mallin et al. 2006

North Carolina Pages Creek Documented 2000 Combination of agricultural, urban, suburban runoff contribute to nutrient loading. MacPherson et al. 2007, Mallin et al. 2006

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 141 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

North Carolina Pamlico River Documented 1960 Mortality of fishes, mass mortality of benthos, low macrobenthic diversity and density in summer, recolonization by winter. Stanley 1985, Tenore 1972, Hobbie et al. 1975, Stanley and Nixon 1992

North Carolina Pamlico Sound Documented 1990 Common finfish species (pinfish, spot, croaker) had skin lesions and signs of systemic bacterial infections. No hypoxia in 1960s through the 1980s, low dissolved oxygen developed in late 1990s. Hurricane increased freshwater and nutrient input. Paerl et al. 2000, Stanley 1985

North Carolina Pasquotank River None 1980 Stanley 1985

North Carolina Perquimans River None 1980 Stanley 1985

North Carolina Pungo River None 1980 Stanley 1985

North Carolina Six Runs Creek Documented 2000 Combination of agricultural, urban, suburban runoff contribute to nutrient loading. Mallin et al. 2006

North Carolina Smith Creek Documented 2000 Combination of agricultural, urban, suburban runoff contribute to nutrient loading. Mallin et al. 2006

North Carolina White Oak River None 1980 Stanley 1985

South Carolina Ashley River None 1980 Stanley 1985

South Carolina Beresford Creek Documented 1990 Lerberg et al. 2000

South Carolina Broad River Documented 2000 Dissolved oxygen improved from 1990s to 2000s. Bricker et al. 1999, 2007 South Carolina Bull Creek Documented 1990 Lerberg et al. 2000

South Carolina Bulls Bay None 1980 Stanley 1985 South Carolina Charleston Harbor Documented 2000 Improved wastewater treatment, phosphate ban. Improvement in dissolved oxygen from 1990s to 2000s. Bricker et al. 1999, 2007, Windsor 1985

South Carolina Combahee River None 1980 Stanley 1985

South Carolina Cooper River Concern 1980 Whitledge 1985 South Carolina Coosaw River None 1980 Stanley 1985 South Carolina Copper River Concern 1980 Stanley 1985 South Carolina Deep Creek Documented 1990 Lerberg et al. 2000 South Carolina Diesel Creek Documented 1990 Lerberg et al. 2000 South Carolina Foster Creek Documented 1990 Lerberg et al. 2000 South Carolina James Island Creek Documented 1990 Avoidance by fishes, mortality of benthos. Burnett 1997

South Carolina Kiawah River None 1980 Stanley 1985 South Carolina Little River Inlet None 1980 Stanley 1985

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 142 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

South Carolina Long Bay Documented 2000 Mortality of flounder. Recently documented hypoxia may be related to multiple factors including high chlorophyll a levels. Koepfler et al. 2007

South Carolina Malind Creek Documented 2000 Gillett et al. 2005 South Carolina Murrells Inlet None 1980 Stanley 1985 South Carolina New Market Creek Documented 1990 Lerberg et al. 2000

South Carolina North Edisto River Concern 1980 Stanley 1985, Bricker et al. 2007 South Carolina North Inlet Documented 2000 Stanley 1985, Sanger et al. 2002 South Carolina North Santee River None 1980 Stanley 1985

South Carolina Okatee Creek Documented 2000 Gillett et al. 2005 South Carolina Orange Grove Creek Documented 1990 Lerberg et al. 2000

South Carolina Pee Dee River Concern 1980 Stanley 1985 South Carolina Port Royal Sound None 1980 Stanley 1985

South Carolina Rathall Creek Documented 1990 Lerberg et al. 2000 South Carolina Santee River Concern 1990 Rabalais 1998, Bricker et al. 2007
South Carolina Shem Creek Documented 1990 Lerberg et al. 2000

South Carolina South Atlantic Bight Concern 1970 Dissolved oxygen around 3 mg/l at 100 m depth in 1973- 74. Stanley 1985

South Carolina South Edisto River None 1980 Stanley 1985

South Carolina South Santee River None 1980 Stanley 1985

South Carolina St. Helena Sound Documented 1980 Stanley 1985, Bricker et al. 2007 South Carolina Stono River Documented 1990 No hypoxia in 1980s, low dissolved oxygen developed in 1990s. Stanley 1985, Bricker et al. 1999, 2007 South Carolina Wando River Concern 1980 Stanley 1985, Whitledge 1985 South Carolina Winyah Bay Documented 1980 Stanley 1985, Bricker et al. 2007 

### Pacific Coast Region and Hawaii

Alaska Ward Cove Documented 1950 Periodic fish kill in 1950s. Related to pulp mill discharge 1954-1997, circulation is pore in cove, dissolved oxygen still low in areas. Karna 2003

California Alamitos Bay Documented 2000 Rabalais 1998 California Anaheim Bay to Balsa Chica Concern 1990 Rabalais 1998

California Azevedo Pond Documented 1990 Diel cycling, low dissolved oxygen related to high primary production. Beck and Bruland 2000

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 143 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

California Big Lagoon Documented 1980 Collias 1985, Engle et al. 1999 California Coyote Creek Documented 1970 Fisherman reported absence of fish and pelagic invertebrates, fish returned after hypoxia ended. Sewage spill. Cloern and Oremland 1983

California Drakes Estero None 1980 Collias 1985 California Eel River None 1990 Rabalais 1998, Bricker et al. 2007 California Elkhorn Slough Documented 1990 Dissolved oxygen improved from 1990s to 2000s. Bricker et al. 1999, 2007, Sanger et al. 2002 California Elkhorn Slough, Upper Pond Documented 2000 Bricker et al. 1999, 2007, Sanger et al. 2002 California Humboldt Bay/ Arcata Bay None 1980 Collias 1985

California Klamath River None 1990 Rabalais 1998, Bricker et al. 2007
California Long Beach Harbor Documented 1960 Whitledge 1985

California Los Angeles Harbor Documented 1950 Mass mortality ofbenthos with multiyear recovery. Improved from nutrient management. Reish 1955, 2000

California Mission Bay None 1990 Rabalais 1998, Bricker et al. 2007 California Monteray Bay Documented 2000 Combination of anthropogenic and natural factors. Okey 2003

California Morro Bay None 1990 Rabalais 1998, Bricker et al. 2007
California Newport Bay Documented 1980 Dissolved oxygen improved from 1990s to 2000s. Collias 1985, Rabalais 1998 California North San Francisco Bay Estuary Documented 2000 Fish Kill Anoxia develops. Lehman et al. 2004

California Palos Verde Shelf None 1970 Organic content of sediment enriched from sewage, but no hypoxia. Smith et al. 2001, Weisberg (Personal Communication) California San Diego Bay Documented 1980 Collias 1985, Rabalais 1998

California San Francisco Bay North Concern 1980 Collias 1985

California San Francisco Bay South Documented 1960 Hypoxia eliminated with sewage treatment plants. Recovered from low dissolved oxygen through nutrient management. Nichols et al. 1986, Bricker et al. 2007

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 144 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

California San Joaquin River Documented 1960 Salmon migration blocked when dissolved oxygen <6 mg/l. High bacterial respiration and nitrification make dissolved oxygen low, stratification is weak. Sewage discharge and nonpoint runoff are sources of organic matter, primary production also contributes. First <2 mg/l dissolved oxygen in 1972. Lehman et al. 2004, Bricker et al. 2007, Jassby and Van Nieuwenhuyse 2005

California San Pedro Bay None 1990 Rabalais 1998, Bricker et al. 2007 California Santa Monica Bay Documented 1990 Rabalais 1998, Bricker et al. 2007 California Smith River None 1980 Collias 1985 California Tijuana Estuary Documented 2000 Dissolved oxygen improved from 1990s to 2000s. Bricker et al. 2007, Sanger et al. 2002 California Tomales Bay/ Bodega Harbor Concern 1980 Collias 1985

Hawaii O'ahu, Off Southern Shore Documented 2000 Mortality of benthos with low species richness and abundance. Fish aquaculture. Lee et al. 2006

Oregon Alsea River None 1980 Collias 1985 Oregon California Current System Documented 2000 Mortality of fishes and mass mortality of benthos. Shifting wind patterns lead to extensive shallow shelf hypoxia. Related to upwelling and phytoplankton bloom. Hypoxia developed on inner shelf in 2000s. Grantham et al. 2004, Chan et al. 2008

Oregon Columbia River None 1980 Collias 1985, Keefer et al. 2008 Oregon Coos Bay None 1980 Collias 1985 Oregon Coquille River None 1980 Collias 1985 Oregon Nehalem River None 1980 Collias 1985 Oregon Nestucca Bay None 1980 Collias 1985 Oregon Netarts Bay None 1980 Collias 1985 Oregon Rogue River Documented 1980 Collias 1985, Sanger et al. 2002 Oregon Siletz Bay None 1980 Collias 1985 Oregon Siuslaw River None 1980 Collias 1985 Oregon South Slough Documented 1990 Daily cycle of dissolved oxygen from supersaturated to hypoxic. Sanger et al. 2002

Oregon Tillamook Bay None 1980 Collias 1985 Oregon Umpqua River Concern 1970 1970s intrusion of low dissolved oxygen (<2 mg/l). No hypoxia in 1980s. Collias 1985, Brown et al. 2007

Oregon Yaquina Bay Documented 2000 No hypoxia in 1980s. Low dissolved oxygen water at times advected into estuary from shelf. Collias 1985, Brown et al. 2007

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 145 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Washington Admiralty Inlet Concern 1990 Washington State Dept. Ecol. 2002 Washington Bellingham Bay Concern 1990 No hypoxia in 1980s. Collias 1985, Washington State Dept. Ecol. 2002 Washington Budd Inlet Documented 1980 Whitledge 1985, Washington State Dept. Ecol. 2002 Washington Commencement Bay Concern 1990 Washington State Dept. Ecol. 2002 Washington Dana Passage None 1990 Washington State Dept. Ecol. 2002 Washington Discovery Bay Documented 1990 Washington State Dept. Ecol. 2002 Washington DRA002 None 1990 Washington State Dept. Ecol. 2002 Washington ELD002 None 1990 Washington State Dept. Ecol. 2002 Washington Elliott Bay Documented 1990 Washington State Dept. Ecol. 2002 Washington GOR001 None 1990 Washington State Dept. Ecol. 2002 Washington Grays Harbor Concern 1980 Collias 1985 Washington Hood Canal Documented 1930 Fish did not enter dissolved oxygen minimum later in water column while invertebrates did. Dissolved oxygen declined from 1990s to 2000s. Paulson et al. 1993, Bricker et al. 2007, Parker-Stetter and Horne 2008

Washington Lynch Cove Documented 1980 Low dissolved oxygen all year round. Whitledge 1985, Washington State Dept. Ecol. 2002 Washington Oakland Bay None 1990 Washington State Dept. Ecol. 2002 Washington Padilla Bay Documented 1990 No hypoxia in 1980s, Low dissolved oxygen in submerged aquatic vegetation bed. Collias 1985, Sanger et al. 2002

Washington Penn Cove Documented 2000 Washington State Dept. Ecol. 2002 Washington POD007 None 1990 Washington State Dept. Ecol. 2002 Washington Port Gamble Concern 1990 Washington State Dept. Ecol. 2002 Washington Port Gardner None 1980 Collias 1985 Washington Port Orchard System Concern 1980 Collias 1985

Washington Port Susan Documented 1980 Collias 1985 Washington Possession Sound Concern 1990 Washington State Dept. Ecol. 2002 Washington Puget Sound None 1990 Rabalais 1998, Bricker et al. 2007 Washington Samish Bay Concern 1980 No hypoxia in 1980s. Collias 1985 Washington Saratoga Passage Documented 1990 Washington State Dept. Ecol. 2002

### Scientific Assessment of Hypoxia in U.S. Coastal Waters 146 Appendix III. Table of Systems

State System Condition Decade Condition First Recorded Faunal Response Trends and Other Comments Reference

Washington Sequim/ Discovery Bays Documented 1980 Collias 1985

Washington Sinclair Inlet Concern 1990 Washington State Dept. Ecol. 2002 Washington Skagit Bay Documented 1990 Rabalais 1998 Washington South Puget Sound Documented 1980 Collias 1985

Washington Straight of Georgia Concern 1990 Washington State Dept. Ecol. 2002 Washington TOT001 None 1990 Washington State Dept. Ecol. 2002 Washington West Point Documented 1990 Washington State Dept. Ecol. 2002 Washington Whidbey Basin Documented 1980 Collias 1985, Rabalais 1998 Washington Willapa Bay None 1980 Collias 1985 Washington Willapa River Documented 1990 Washington State Dept. Ecol. 2002 

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