# Patent

## Title
Glucoamylase variants with altered properties

## Abstract

The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.

## Background
CROSS REFERENCE TO RELATED APPLICATIONS
This application is a Divisional of U.S. patent application Ser. No. 13/251,116, filed Sep. 30, 2011, now U.S. Pat. No. 8,679,792, which is a Continuation of U.S. patent application Ser. No. 12/292,563, filed Nov. 20, 2008, now U.S. Pat. No. 8,058,033, which claims benefit to U.S. Provisional Patent Application No. 60/989,426 filed Nov. 20, 2007, the disclosures of which are incorporated herein by reference in their entirety for all purposes.


SEQUENCE LISTING
The sequence listing submitted via EFS, in compliance with 37 C.F.R. §1.52(e), is incorporated herein by reference. The sequence listing text file submitted via EFS contains the file “31060USC1D1-SEQLIST.txt”, created on Apr. 9, 2014, which is 98,304 bytes in size.
FIELD OF THE INVENTION
Glucoamylase variants advantageously have altered properties (e.g., improved thermostability and/or specific activity). Compositions comprising the variant glucoamylases, DNA constructs encoding the variants, and methods of producing the glucoamylase variants in host cells are provided.
BACKGROUND
Glucoamylase enzymes (glucan 1,4-α-glucohydrolases, EC 3.2.1.3) are starch hydrolyzing exo-acting carbohydrases, which catalyze the removal of successive glucose units from the non-reducing ends of starch or related oligo and polysaccharide molecules. Glucoamylases can hydrolyze both the linear and branched glucosidic linkages of starch (e.g., amylose and amylopectin).
Glucoamylases are produced by numerous strains of bacteria, fungi, yeast and plants. Particularly interesting, and commercially important, glucoamylases are fungal enzymes that are extracellularly produced, for example from strains of Aspergillus (Svensson et al. (1983) Carlsberg Res. Commun. 48:529-544; Boel et al., (1984) EMBO J. 3:1097-1102; Hayashida et al., (1989) Agric. Biol. Chem. 53:923-929; U.S. Pat. No. 5,024,941; U.S. Pat. No. 4,794,175 and WO 88/09795); Talaromyces (U.S. Pat. No. 4,247,637; U.S. Pat. No. 6,255,084 and U.S. Pat. No. 6,620,924); Rhizopus (Ashikari et al., (1986) Agric. Biol. Chem. 50:957-964; Ashikari et al., (1989) App. Microbiol. Biotech. 32:129-133 and U.S. Pat. No. 4,863,864); Humicola (WO 05/052148 and U.S. Pat. No. 4,618,579) and Mucor (Houghton-Larsen et al., (2003) Appl. Microbiol. Biotechnol. 62:210-217). Many of the genes that code for these enzymes have been cloned and expressed in yeast, fungal and/or bacterial cells.
Commercially, glucoamylases are very important enzymes and have been used in a wide variety of applications that require the hydrolysis of starch (e.g., for producing glucose and other monosaccharides from starch). Glucoamylases are used to produce high fructose corn sweeteners, which comprise over 50% of the sweetener market in the United States. In general, glucoamylases may be, and commonly are, used with alpha amylases in starch hydrolyzing processes to hydrolyze starch to dextrins and then glucose. The glucose may then be converted to fructose by other enzymes (e.g., glucose isomerases); crystallized; or used in fermentations to produce numerous end products (e.g., ethanol, citric acid, lactic acid, succinate, ascorbic acid intermediates, glutamic acid, glycerol and 1,3-propanediol). Ethanol produced by using glucoamylases in the fermentation of starch and/or cellulose containing material may be used as a source of fuel or for alcoholic consumption.
Although glucoamylases have been used successfully in commercial applications for many years, a need still exists for new glucoamylases with altered properties, such as improved specific activity and increased thermostability.
Different mutations have been made in glucoamylases of Aspergillus that enhance thermal stability and specific activity. Reference is made to U.S. Pat. No. 6,537,792; U.S. Pat. No. 6,352,851; Chen et al. (1996) Prot. Eng. 9:499-505, Chen et al., (1995) Prot Eng. 8:575-582; Fierobe et al. (1996) Biochem. 35:8698-8704; and Li et al., (1997) Prot. Eng. 10:1199-1204. The need still exists for providing glucoamylase variants with altered properties relative to their parent.
SUMMARY
The present disclosure relates to glucoamylase variants of a parent glucoamylase. The glucoamylase variants contain amino acid substitutions with in the catalytic domain and/or the starch-binding domain. The variants display altered properties, such as improved thermostability and/or specific activity.
In one aspect, the present disclosure relates to a glucoamylase variant comprising two or more amino acid substitutions corresponding to position 61, 73, 417, 430, 431, 503, 511, 535, 539, or 563 of SEQ ID NO: 2, or an equivalent position in a parent glucoamylase. In another aspect, the present disclosure relates a glucoamylase variant having at least 80%, 85%, 90%, 95%, or 99.5% sequence identity with a parent glucoamylase of SEQ ID NO: 1, 2, 3, 5, 6, 7, 8, or 9. In one embodiment, the parent glucoamylase has a catalytic domain with at least 80% sequence identity with SEQ ID NO: 1, 2, 3, 5, 6, 7, 8, or 9, or a starch binding domain with at least 80% sequence identity with SEQ ID NO: 1 or 2. In other aspect, the parent glucoamylase is SEQ ID NO: 1 or 2. A further aspect of the present disclosure relates to glucoamylase variant further comprising one or more amino acid substitutions corresponding to position: 4, 5, 12, 24, 43, 44, 45, 46, 47, 49, 51, 70, 75, 6, 94, 100, 108, 114, 116, 119, 122, 124, 125, 137, 141, 143, 146, 148, 169, 171, 172, 175, 178, 180, 181, 208, 211, 228, 242, 243, 245, 292, 294, 197, 309, 310, 313, 314, 315, 316, 317, 321, 340, 341, 350, 353, 356, 363, 368, 369, 375, 376, 395, 398, 401, 408, 409, 412, 415, 418, 421, 433, 436 or 451 of SEQ ID NO: 2, or an equivalent position in the parent glucoamylase. In some aspects, the glucoamylase variant further comprises one or more amino acid substitutions corresponding to position: 4, 5, 24, 29, 43, 44, 49, 70, 75, 76, 100, 108, 119, 124, 137, 146, 148, 169, 171, 172, 175, 178, 181, 208, 211, 243, 292, 294, 297, 314, 316, 317, 340, 341, 350, 356, 363, 368, 369, 376, 395, 401, 412, 433, 436 or 451 of SEQ ID NO: 2, or an equivalent position in a parent glucoamylase. In some aspects, the glucoamylase further comprises one or more amino acid substitutions corresponding to position: 5, 24, 43, 44, 49, 70, 75, 76, 94, 119, 141, 146, 148, 172, 175, 178, 180, 181, 208, 211, 243, 294, 309, 314, 353, 369, 375, or 409 of SEQ ID NO: 2, or an equivalent position in a parent glucoamylase. In some aspects, the glucoamylase further comprises one or more amino acid substitutions corresponding to position: 43, 44, or 294 of SEQ ID NO: 2, or an equivalent position in a parent glucoamylase.
In further aspects of the invention, the glucoamylase variant comprises two or more amino acid substitutions corresponding to position: N61I, G73F, L417R/V, T430A/M, A431L/Q, E503A/V, Q511H, A535R, A539R, or N563I/K of SEQ ID NO: 2, or an equivalent position in a parent glucoamylase. In some aspects, the glucoamylase variant further comprises one or more of the following substitutions: D4L/E/R/S/C/A/Q/W, F5C/M/N/R/S/T/V/W, 112L/R, D24E/L/Y/T, F29L/I/D/C/S/V/W, I43F/R/D/Y/S/Q, D44E/H/K/S/N/Y/F/R/C, Y47W, Y49N, Q70R/K/M/P/G/L/F, Q75R/K/A, R76L/M/K/T/P, P94L, D100W/I/Q/M/P/A/N, N119P/T/Y/D/E, N146S/G/C/H/E/D/T/W/L/F/M, Q148V/Y/H/A/C/D/G/M/R/S/T, Y169D/F, Q172C/A/D/R/E/F/H/V/L/M/N/S/T/V, F175H/A/G/R/S/T/C/W/Y, W178A/C/D/E/F/G/H/K/N/R/S/T/V/Y, E180A/C/G/H/I/L/N/P/Q/R/S/TN/Y/, V181E/C/D/G/H/I/P/T/Y/S/L/K/F/A, Q208L/A/C/E/N/F/H/T, S211C/R/E/A/Y/W/M/H/L/I/R/Q/T, E243S/R/N/M/Y/A/L, R245A/E/M/I/P/V, I292D/H/P/R/T/N/V/F/L, G294C/D/E/T/Q/I/A, K297F/L/P/T/M/D/N/Q/A/Y/H/S/R/W, R309A/C/G/H/I/N/P/Q/S/T/W/Y/L, Y310E/G/L/P/S/W/R/Q, D313Q, V314A/R/N/D/C/E/Q/G/H/I/L/K/M/F/P/S/T/W/Y, Y315F, Y316Q/R, N317T/H, K340D/T, K341F/D/P/V/G/S, T350S/E/A/N, Q356H/D/E, T363L/R/C/H/W, S368W/D/F/L, S369F, N376Q/T/H/S/V, Y395Q/R/S, A398S/I/T, S401C/V, R408S, N409W/T/K, T412A/H/K/G, R433H/Q, I436A/T, or S451M/T/H of SEQ ID NO: 2, or an equivalent position in a parent glucoamylase. In some aspects, the glucoamylase variant further comprises one or more of the following substitutions: I43F/R/D/Y/S/Q, D44E/H/K/S/N/Y/F/R/C, or G294C/D/E/T/Q/I/A of SEQ ID NO: 2, or an equivalent position in a parent glucoamylase.
In one aspect, the present disclosure relates a variant glucoamylase comprises comprising amino acid substitutions corresponding to positions: I43Q/D44C, D44C/G294C, I43Q/G294C, or I43Q/D44C/G294 of SEQ ID NO: 2, or an equivalent position in a parent glucoamylase. The glucoamylase variant has at least 80%, 85%, 90%, 95%, or 99.5% sequence identity with SEQ ID NO: 1, 2, 3, 5, 6, 7, 8, or 9. In one embodiment, the parent glucoamylase has a catalytic domain with at least 80% sequence identity with SEQ ID NO: 1, 2, 3, 5, 6, 7, 8, or 9, or a starch binding domain with at least 80% sequence identity with SEQ ID NO: 1 or 2.
The parent glucoamylase can the enzyme obtained from any of: a Trichoderma spp., an Aspergillus spp., a Humicola spp., a Penicillium spp., a Talaromycese spp., or a Schizosaccharmyces spp. In some aspects, the parent glucoamylase can be from a Trichoderma spp. or an Aspergillus spp.
In one aspect, the variant glucoamylase exhibits altered thermostability as compared to the parent glucoamylase. The altered thermostability can be increased thermostability. Alternatively, or in addition, the variant exhibits altered specific activity compared to the parent glucoamylase. The altered specific activity can be increased specific activity.
A further aspect of the disclosure is a polynucleotide encoding the variant described. A further aspect is a vector comprising the polynucleotide. A further aspect is a host cell containing the vector.
A further aspect of the disclosure is an enzyme composition including the glucoamylase variant. In one aspect, the enzyme composition is used in a starch conversion process or an alcohol fermentation process.
A further aspect of the invention is a method of producing a variant glucoamylase by culturing the host cell containing the polynucleotide under conditions suitable for the expression and production of the glucoamylase variant and producing the variant. The method may also include the step of recovering the glucoamylase variant from the culture.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1A depicts the Trichoderma reesei glucoamylase (TrGA) parent glucoamylase having 632 amino acids (SEQ ID NO: 1). The signal peptide is underlined, the catalytic region (SEQ ID NO: 3) starting with amino acid residues SVDDFI (SEQ ID NO: 160) and having 453 amino acid residues is in bold; the linker region is in italics; and the starch binding domain (SEQ ID NO: 161) is both italics and underlined. The mature protein, which includes the catalytic domain (SEQ ID NO: 3), linker region, and starch binding domain (SEQ ID NO: 161), is represented by SEQ ID NO: 2. FIG. 1B depicts the cDNA (SEQ ID NO: 4) that codes for the TrGA.
FIG. 2 depicts the plasmid pDONR-TrGA that includes the cDNA (SEQ ID NO: 4) of the parent TrGA.
FIG. 3 depicts the plasmids pREP3Y-DEST (A) and pREP3Y-TrGA (B).
FIGS. 4A-4B depict an alignment comparison of the catalytic domains of parent glucoamylases including glucoamylase derived from Aspergillus awamori (AaGA) (SEQ ID NO: 5); Aspergillus niger (AnGA) (SEQ ID NO: 6); Aspergillus orzyae (AoGA) (SEQ ID NO: 7); Trichoderma reesei (TrGA) (SEQ ID NO: 3); Humicola grisea (HgGA) (SEQ ID NO: 8); and Hypocrea vinosa (HvGA) (SEQ ID NO: 9). Identical amino acids are indicated by an asterisk (*).
FIGS. 4C-4D depict an alignment comparing the Starch Binding Domain (SBD) of parent glucoamylases including Trichoderma reesei (TrGA) (SEQ ID NO: 161), Humicola grisea (HgGA) (SEQ ID NO: 162), Thielavia terrestris (TtGA) (SEQ ID NO: 163), Thermomyces lanuginosus (ThGA) (SEQ ID NO: 164), Talaromyces emersonii (TeGA) (SEQ ID NO: 165), Aspergillus niger (AnGA) (SEQ ID NO: 166), and Aspergillus awamori (AaGA) (SEQ ID NO: 167). Identical amino acids are indicated by a dot (.).
FIG. 5A depicts the plasmid pTrex3g-DEST. FIG. 5B depicts the plasmid pTrex3g-TrGA. The plasmids were used as expression vectors for expression and production of variant glucoamylases in a Trichoderma reesei host.
FIGS. 6A and 6B depict the Vmax (μM glucose/sec) comparison between the parent (wild-type) TrGA and variants, V314, S211R, Q172F, and Q208N at 60° C. (FIG. 6A) and 32° C. (FIG. 6B) as further discussed in Example 8.
FIG. 7 depicts the activity of combinatorial variants on a starch substrate. Combinatorial variants described herein include ET7-1 (D24Y/V181L/Q208C/G294A/T353R/N375N/N409W), LR8 (Q172F/Q208N), LR12 (Q172F/S211R), LR6 (Q172F/Q208N/V314H), ET8-1 (D24E/V181K/E243Y/I292V/G294Q/N409K), and ET7-2 (Q24L/V181L/Q208C/G294A/T353R/N375Q/N409W). Activity is represented in units of absorption at 340 nm as a function of ng of indicated glucoamylase variants.
FIG. 8 depicts the activity of single site variants on a cornstarch substrate. Single site variants described herein include V314H, G294Q, S211R, Q208N, Q172F, G2941, and P94N. Activity is represented in units of absorption at 340 nm as a function of ng of indicated glucoamylase variants.
FIG. 9 depicts the glucoamylase activity of TrGA and the TrGA variant LR8 (Q172F/Q208N) on a sample of corn mash liquefact (NE).
FIG. 10 depicts the activity profile of TrGA and the TrGA variant LR8 (Q172F/Q208N) on a sample of corn mash liquefact (BSE).
FIG. 11 depicts the activity profile of TrGA and the TrGA variant LR8 (Q172F/Q208N) on a sample soluble cornstarch substrate.
FIG. 12 depicts a comparison of the three dimensional structures of Trichoderma reesei glucoamylase (black) (SEQ ID NO: 2) and Aspergillus awamorii glucoamylase (grey) viewed from the side.
FIG. 13 depicts a comparison of the three dimensional structures of Trichoderma reesei glucoamylase (black) and Aspergillus awamorii glucoamylase (grey) viewed from the top.
FIG. 14A depicts the plasmid pTTT-Dest. FIG. 14B depicts the plasmid pTTT-TrGA(B).



DETAILED DESCRIPTION
Glucoamylases are commercially important enzymes in a wide variety of applications that require the hydrolysis of starch. Glucoamylases variants of described herein contains amino acid substitutions within the catalytic domain or the starch binding domain. The variants may display altered properties such as improved thermostability and/or specific activity. The variants with improved thermostability and/or specific activity may significantly improve the efficiency of glucose and fuel ethanol production from corn starch, for example.
1. Definitions
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. Singleton, et al., DICTIONARY OF MICROBIOLOGY AND MOLECULAR BIOLOGY, 2D ED., John Wiley and Sons, New York (1994), and Hale & Markham, THE HARPER COLLINS DICTIONARY OF BIOLOGY, Harper Perennial, N.Y. (1991) provide one of skill with the general meaning of many of the terms used herein. Certain terms are defined below for the sake of clarity and ease of reference.
As used herein, the term “glucoamylase (EC 3.2.1.3)” refers to an enzyme that catalyzes the release of D-glucose from the non-reducing ends of starch and related oligo- and polysaccharides.
The term “parent” or “parent sequence” refers to a sequence that is native or naturally occurring in a host cell. Parent sequences include, but are not limited to, the glucoamylase sequences set forth in SEQ ID NOs: 1, 2, 3, 5, 6, 7, 8, and 9.
As used herein, an “equivalent position” means a position that is common to two parent sequences that is based on an alignment of the amino acid sequence of the parent glucoamylase in question as well as alignment of the three-dimensional structure of the parent glucoamylase in question with the TrGA reference glucoamylase amino acid sequence (SEQ ID NO: 2) and three-dimensional sequence.
The term “TrGA” refers to a parent Trichoderma reesei glucoamylase sequence having the mature protein sequence illustrated in SEQ ID NO: 2 that includes the catalytic domain having the sequence illustrated in SEQ ID NO: 3. The isolation, cloning and expression of the TrGA are described in U.S. Pat. No. 7,413,887, which are incorporated herein by reference. In some embodiments, the parent sequence refers to a glucoamylase that is the starting point for protein engineering. The numbering of the glucoamylase amino acids herein is based on the sequence alignment of a glucoamylase with TrGA (SEQ ID NO: 2 and SEQ ID NO: 3).
The phrase “mature form of a protein or polypeptide” refers to the final functional form of the protein or polypeptide. To exemplify, a mature form of the TrGA includes the catalytic domain, linker region, and starch binding domain having the amino acid sequence of SEQ ID NO: 2.
As used herein, the terms “glucoamylase variant” and “variant” are used in reference to glucoamylases that have some degree of amino acid sequence identity to a parent glucoamylase sequence and that may retain the functional characteristics of a glucoamylase. A variant is similar to a parent sequence, but has at least one substitution, deletion or insertion in their amino acid sequence that makes them different in sequence from a parent glucoamylase. In some cases, variants have been manipulated and/or engineered to include at least one substitution, deletion, or insertion in their amino acid sequence that makes them different in sequence from a parent.
“Variants” may have at least 99.5%, at least 99%, at least 98%, at least 97%, at least 96%, at least 95%, at least 94%, at least 93%, at least 92%, at least 91%, at least 90%, at least 88%, at least 85%, at least 80%, at least 75%, at least 70%, at least 65%, at least 60%, at least 55%, at least 50%, or at least 45% sequence identity to a polypeptide sequence when optimally aligned for comparison. In some embodiments, the glucoamylase variant may have at least 99.5%, at least 99%, at least 98%, at least 97%, at least 96%, at least 95%, at least 94%, at least 93%, at least 92%, at least 91%, at least 90%, at least 88%, at least 85%, at least 80%, at least 75%, at least 70%, at least 65%, at least 60%, at least 55%, at least 50%, or at least 45% sequence identity to the catalytic domain of a parent glucoamylase. In some embodiments, the glucoamylase variant may have at least 99.5%, at least 99%, at least 98%, at least 97%, at least 96%, at least 95%, at least 94%, at least 93%, at least 92%, at least 91%, at least 90%, at least 88%, at least 85%, at least 80%, at least 75%, at least 70%, at least 65%, at least 60%, at least 55%, at least 50%, or at least 45% sequence identity to the starch binding domain of a parent glucoamylase. The sequence identity can be measured over the entire length of the parent or the variant sequence.
Sequence identity is determined using standard techniques known in the art (see e.g., Smith and Waterman, Adv. Appl. Math. 2: 482 (1981); Needleman and Wunsch, J. Mol. Biol. 48: 443 (1970); Pearson and Lipman, Proc. Natl. Acad. Sci. USA 85: 2444 (1988); programs such as GAP, BESTHT, FASTA, and TFASTA in the Wisconsin Genetics Software Package (Genetics Computer Group, Madison, Wis.); and Devereux et al., Nucleic Acid Res., 12: 387-395 (1984)).
The “percent (%) nucleic acid sequence identity” or “percent (%) amino acid sequence identity” is defined as the percentage of nucleotide residues or amino acid residues in a candidate sequence that are identical with the nucleotide residues or amino acid residues of the starting sequence (e.g., TrGA). The sequence identity can be measured over the entire length of the starting sequence (e.g., SEQ ID NO: 2)
Sequence identity is determined by known methods of sequence alignment. A commonly used alignment method is BLAST described by Altschul et al., (Altschul et al., J. Mol. Biol. 215: 403-410 (1990); and Karlin et al, Proc. Natl. Acad. Sci. USA 90: 5873-5787 (1993)). A particularly useful BLAST program is the WU-BLAST-2 program (see Altschul et al, Meth. Enzymol. 266: 460-480 (1996)). WU-BLAST-2 uses several search parameters, most of which are set to the default values. The adjustable parameters are set with the following values: overlap span=1, overlap fraction=0.125, word threshold (T)=11. The HSP S and HSP S2 parameters are dynamic values and are established by the program itself depending upon the composition of the particular sequence and composition of the particular database against which the sequence of interest is being searched. However, the values may be adjusted to increase sensitivity. A % amino acid sequence identity value is determined by the number of matching identical residues divided by the total number of residues of the “longer” sequence in the aligned region. The “longer” sequence is the one having the most actual residues in the aligned region (gaps introduced by WU-Blast-2 to maximize the alignment score are ignored).
Other methods find use in aligning sequences. One example of a useful algorithm is PILEUP. PILEUP creates a multiple sequence alignment from a group of related sequences using progressive, pair-wise alignments. It can also plot a tree showing the clustering relationships used to create the alignment. PILEUP uses a simplification of the progressive alignment method of Feng and Doolittle (Feng and Doolittle, J. Mol. Evol. 35: 351-360 (1987)). The method is similar to that described by Higgins and Sharp (Higgins and Sharp, CABIOS 5: 151-153 (1989)). Useful PILEUP parameters including a default gap weight of 3.00, a default gap length weight of 0.10, and weighted end gaps.
The term “optimal alignment” refers to the alignment giving the highest percent identity score.
As used herein the term “catalytic domain” refers to a structural region of a polypeptide, which contains the active site for substrate hydrolysis.
The term “linker” refers to a short amino acid sequence generally having between 3 and 40 amino acids residues that covalently bind an amino acid sequence comprising a starch binding domain with an amino acid sequence comprising a catalytic domain.
The term “starch binding domain” refers to an amino acid sequence that binds preferentially to a starch substrate.
As used herein, the terms “mutant sequence” and “mutant gene” are used interchangeably and refer to a polynucleotide sequence that has an alteration in at least one codon occurring in a host cell's parent sequence. The expression product of the mutant sequence is a variant protein with an altered amino acid sequence relative to the parent. The expression product may have an altered functional capacity (e.g., enhanced enzymatic activity).
The term “property” or grammatical equivalents thereof in the context of a polypeptide, as used herein, refers to any characteristic or attribute of a polypeptide that can be selected or detected. These properties include, but are not limited to oxidative stability, substrate specificity, catalytic activity, thermal stability, pH activity profile, resistance to proteolytic degradation, KM, KCAT, KCAT/KM ratio, protein folding, ability to bind a substrate and ability to be secreted.
The term “property” of grammatical equivalent thereof in the context of a nucleic acid, as used herein, refers to any characteristic or attribute of a nucleic acid that can be selected or detected. These properties include, but are not limited to, a property affecting gene transcription (e.g., promoter strength or promoter recognition), a property affecting RNA processing (e.g., RNA splicing and RNA stability), a property affecting translation (e.g., regulation, binding of mRNA to ribosomal proteins).
The terms “thermally stable” and “thermostable” refer to glucoamylase variants of the present disclosure that retain a specified amount of enzymatic activity after exposure to a temperature over a given period of time under conditions prevailing during the hydrolysis of starch substrates, for example, while exposed to altered temperatures.
The term “enhanced stability” in the context of a property such as thermostability refers to a higher retained starch hydrolytic activity over time as compared to another reference (i.e., parent) glucoamylases.
The term “diminished stability” in the context of a property such as thermostability refers to a lower retained starch hydrolytic activity over time as compared to another reference glucoamylase.
The term “specific activity” is defined as the activity per mg of glucoamylase protein. In some embodiments, the activity for glucoamylase is determined by the ethanol assay described herein and expressed as the amount of glucose that is produced from the starch substrate. In some embodiments, the protein concentration can be determined using the Caliper assay described herein.
The terms “active” and “biologically active” refer to a biological activity associated with a particular protein. It follows that the biological activity of a given protein refers to any biological activity typically attributed to that protein by those skilled in the art. For example, an enzymatic activity associated with a glucoamylase is hydrolytic and, thus an active glucoamylase has hydrolytic activity.
The terms “polynucleotide” and “nucleic acid”, used interchangeably herein, refer to a polymeric form of nucleotides of any length, either ribonucleotides or deoxyribonucleotides. These terms include, but are not limited to, a single-, double- or triple-stranded DNA, genomic DNA, cDNA, RNA, DNA-RNA hybrid, or a polymer comprising purine and pyrimidine bases, or other natural, chemically, biochemically modified, non-natural or derivatized nucleotide bases.
As used herein, the terms “DNA construct,” “transforming DNA” and “expression vector” are used interchangeably to refer to DNA used to introduce sequences into a host cell or organism. The DNA may be generated in vitro by PCR or any other suitable technique(s) known to those in the art. The DNA construct, transforming DNA or recombinant expression cassette can be incorporated into a plasmid, chromosome, mitochondrial DNA, plastid DNA, virus, or nucleic acid fragment. Typically, the recombinant expression cassette portion of an expression vector, DNA construct or transforming DNA includes, among other sequences, a nucleic acid sequence to be transcribed and a promoter. In some embodiments, expression vectors have the ability to incorporate and express heterologous DNA fragments in a host cell.
As used herein, the term “vector” refers to a polynucleotide construct designed to introduce nucleic acids into one or more cell types. Vectors include cloning vectors, expression vectors, shuttle vectors, plasmids, cassettes, and the like.
As used herein in the context of introducing a nucleic acid sequence into a cell, the term “introduced” refers to any method suitable for transferring the nucleic acid sequence into the cell. Such methods for introduction include but are not limited to protoplast fusion, transfection, transformation, conjugation, and transduction.
As used herein, the terms “transformed” and “stably transformed” refers to a cell that has a non-native (heterologous) polynucleotide sequence integrated into its genome or as an episomal plasmid that is maintained for at least two generations.
As used herein, the terms “selectable marker” and “selective marker” refer to a nucleic acid (e.g., a gene) capable of expression in host cells that allows for ease of selection of those hosts containing the vector. Typically, selectable markers are genes that confer antimicrobial resistance or a metabolic advantage on the host cell to allow cells containing the exogenous DNA to be distinguished from cells that have not received any exogenous sequence during the transformation.
As used herein, the term “promoter” refers to a nucleic acid sequence that functions to direct transcription of a downstream gene. The promoter, together with other transcriptional and translational regulatory nucleic acid sequences (also termed “control sequences”) is necessary to express a given gene. In general, the transcriptional and translational regulatory sequences include, but are not limited to, promoter sequences, ribosomal binding sites, transcriptional start and stop sequences, translational start and stop sequences, and enhancer or activator sequences.
A nucleic acid is “operably linked” when it is placed into a functional relationship with another nucleic acid sequence. For example, DNA encoding a secretory leader (i.e., a signal peptide), is operably linked to DNA for a polypeptide if it is expressed as a preprotein that participates in the secretion of the polypeptide. Generally, “operably linked” means that the DNA sequences being linked are contiguous, and, in the case of a secretory leader, contiguous and in reading phase.
As used herein the term “gene” refers to a polynucleotide (e.g., a DNA segment), that encodes a polypeptide and includes regions preceding and following the coding regions, as well as intervening sequences (introns) between individual coding segments (exons).
As used herein, “ortholog” and “orthologous genes” refer to genes in different species that have evolved from a common ancestral gene (i.e., a homologous gene) by speciation. Typically, orthologs retain the same function during the course of evolution. Identification of orthologs finds use in the reliable prediction of gene function in newly sequenced genomes.
As used herein, “paralog” and “paralogous genes” refer to genes that are related by duplication within a genome. While orthologs retain the same function through the course of evolution, paralogs evolve new functions, even though some functions are often related to the original one. Examples of paralogous genes include, but are not limited to genes encoding trypsin, chymotrypsin, elastase, and thrombin, which are all serine proteinases and occur together within the same species.
As used herein, the term “hybridization” refers to the process by which a strand of nucleic acid joins with a complementary strand through base pairing, as known in the art.
A nucleic acid sequence is considered to be “selectively hybridizable” to a reference nucleic acid sequence if the two sequences specifically hybridize to one another under moderate to high stringency hybridization and wash conditions. Hybridization conditions are based on the melting temperature (Tm) of the nucleic acid binding complex or probe. For example, “maximum stringency” typically occurs at about Tm−5° C. (5° below the Tm of the probe); “high stringency” at about 5-10° C. below the Tm; “intermediate stringency” at about 10-20° C. below the Tm of the probe; and “low stringency” at about 20-25° C. below the Tm. Functionally, maximum stringency conditions may be used to identify sequences having strict identity or near-strict identity with the hybridization probe; while an intermediate or low stringency hybridization can be used to identify or detect polynucleotide sequence homologs.
Moderate and high stringency hybridization conditions are well known in the art. An example of high stringency conditions includes hybridization at about 42° C. in 50% formamide, 5×SSC, 5×Denhardt's solution, 0.5% SDS and 100 μg/ml denatured carrier DNA followed by washing two times in 2×SSC and 0.5% SDS at room temperature and two additional times in 0.1×SSC and 0.5% SDS at 42° C. An example of moderate stringent conditions include an overnight incubation at 37° C. in a solution comprising 20% formamide, 5×SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH 7.6), 5×Denhardt's solution, 10% dextran sulfate and 20 mg/ml denaturated sheared salmon sperm DNA, followed by washing the filters in 1×SSC at about 37-50° C. Those of skill in the art know how to adjust the temperature, ionic strength, etc. as necessary to accommodate factors such as probe length and the like.
As used herein, “recombinant” includes reference to a cell or vector, that has been modified by the introduction of a heterologous or homologous nucleic acid sequence or that the cell is derived from a cell so modified. Thus, for example, recombinant cells express genes that are not found in identical form within the native (non-recombinant) form of the cell or express native genes that are otherwise abnormally expressed, under expressed or not expressed at all as a result of deliberate human intervention.
In an embodiment of the disclosure, mutated DNA sequences are generated with site saturation mutagenesis in at least one codon. In another embodiment, site saturation mutagenesis is performed for two or more codons. In a further embodiment, mutant DNA sequences have more than 50%, more than 55%, more than 60%, more than 65%, more than 70%, more than 75%, more than 80%, more than 85%, more than 90%, more than 95%, or more than 98% homology with the parent sequence. In alternative embodiments, mutant DNA is generated in vivo using any known mutagenic procedure such as, for example, radiation, nitrosoguanidine, and the like. The desired DNA sequence is then isolated and used in the methods provided herein.
As used herein, “heterologous protein” refers to a protein or polypeptide that does not naturally occur in the host cell.
An enzyme is “over-expressed” in a host cell if the enzyme is expressed in the cell at a higher level than the level at which it is expressed in a corresponding wild-type cell.
The terms “protein” and “polypeptide” are used interchangeability herein. In the present disclosure and claims, the conventional one-letter and three-letter codes for amino acid residues are used. The 3-letter code for amino acids as defined in conformity with the IUPAC-IUB Joint Commission on Biochemical Nomenclature (JCBN). It is also understood that a polypeptide may be coded for by more than one nucleotide sequence due to the degeneracy of the genetic code.
Variants of the disclosure are described by the following nomenclature: [original amino acid residue/position/substituted amino acid residue]. For example, the substitution of leucine for arginine at position 76 is represented as R76L. When more than one amino acid is substituted at a given position, the substitution is represented as 1) Q172C, Q172D or Q172R; 2) Q172C, D, or R, or 3) Q172C/D/R. When a position suitable for substitution is identified herein without a specific amino acid suggested, it is to be understood that any amino acid residue may be substituted for the amino acid residue present in the position. Where a variant glucoamylase contains a deletion in comparison with other glucoamylases the deletion is indicated with “*”. For example, a deletion at position R76 is represented as R76*. A deletion of two or more consecutive amino acids is indicated for example as (76-78)*.
A “prosequence” is an amino acid sequence between the signal sequence and mature protein that is necessary for the secretion of the protein. Cleavage of the pro sequence will result in a mature active protein.
The term “signal sequence” or “signal peptide” refers to any sequence of nucleotides and/or amino acids that may participate in the secretion of the mature or precursor forms of the protein. This definition of signal sequence is a functional one, meant to include all those amino acid sequences encoded by the N-terminal portion of the protein gene, which participate in the effectuation of the secretion of protein. They are often, but not universally, bound to the N-terminal portion of a protein or to the N-terminal portion of a precursor protein. The signal sequence may be endogenous or exogenous. The signal sequence may be that normally associated with the protein (e.g., glucoamylase), or may be from a gene encoding another secreted protein.
The term “precursor” form of a protein or peptide refers to a mature form of the protein having a prosequence operably linked to the amino or carbonyl terminus of the protein. The precursor may also have a “signal” sequence operably linked, to the amino terminus of the prosequence. The precursor may also have additional polynucleotides that are involved in post-translational activity (e.g., polynucleotides cleaved therefrom to leave the mature form of a protein or peptide).
“Host strain” or “host cell” refers to a suitable host for an expression vector comprising DNA according to the present disclosure.
The terms “derived from” and “obtained from” refer to not only a glucoamylase produced or producible by a strain of the organism in question, but also a glucoamylase encoded by a DNA sequence isolated from such strain and produced in a host organism containing such DNA sequence. Additionally, the term refers to a glucoamylase that is encoded by a DNA sequence of synthetic and/or cDNA origin and that has the identifying characteristics of the glucoamylase in question.
A “derivative” within the scope of this definition generally retains the characteristic hydrolyzing activity observed in the wild-type, native or parent form to the extent that the derivative is useful for similar purposes as the wild-type, native or parent form. Functional derivatives of glucoamylases encompass naturally occurring, synthetically or recombinantly produced peptides or peptide fragments that have the general characteristics of the glucoamylases of the present disclosure.
The term “isolated” refers to a material that is removed from the natural environment if it is naturally occurring.
A “purified” protein refers to a protein that is at least partially purified to homogeneity. In some embodiments, a purified protein is more than 10% pure, optionally more than 20% pure, and optionally more than 30% pure, as determined by SDS-PAGE. Further aspects of the disclosure encompass the protein in a highly purified form (i.e., more than 40% pure, more than 60% pure, more than 80% pure, more than 90% pure, more than 95% pure, more than 97% pure, and even more than 99% pure), as determined by SDS-PAGE.
As used herein, the term, “combinatorial mutagenesis” refers to methods in which libraries of variants of a starting sequence are generated. In these libraries, the variants contain one or several mutations chosen from a predefined set of mutations. In addition, the methods provide means to introduce random mutations that were not members of the predefined set of mutations. In some embodiments, the methods include those set forth in U.S. Pat. No. 6,582,914, hereby incorporated by reference. In alternative embodiments, combinatorial mutagenesis methods encompass commercially available kits (e.g., QuikChange® Multisite, Stratagene, San Diego, Calif.).
As used herein, the term “library of mutants” refers to a population of cells that are identical in most of their genome but include different homologues of one or more genes. Such libraries can be used, for example, to identify genes or operons with improved traits.
As used herein the term “dry solids content (DS or ds)” refers to the total solids of a slurry in % on a dry weight basis.
As used herein, the term “initial hit” refers to a variant that was identified by screening a combinatorial consensus mutagenesis library. In some embodiments, initial hits have improved performance characteristics, as compared to the starting gene.
As used herein, the term “improved hit” refers to a variant that was identified by screening an enhanced combinatorial consensus mutagenesis library.
As used herein, the term “target property” refers to the property of the starting gene that is to be altered. It is not intended that the present disclosure be limited to any particular target property. However, in some embodiments, the target property is the stability of a gene product (e.g., resistance to denaturation, proteolysis or other degradative factors), while in other embodiments, the level of production in a production host is altered. Indeed, it is contemplated that any property of a starting gene will find use in the present disclosure. Other definitions of terms may appear throughout the specification.
Where a range of values is provided, it is understood that each intervening value, to the tenth of the unit of the lower limit unless the context clearly dictates otherwise, between the upper and lower limits of that range is also specifically disclosed. Each smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in that stated range is encompassed within the disclosure. The upper and lower limits of these smaller ranges may independently be included or excluded in the range, and each range where either, neither or both limits are included in the smaller ranges is also encompassed within the disclosure, subject to any specifically excluded limit in the stated range. Where the stated range includes one or both of the limits, ranges excluding either or both of those included limits are also included in the disclosure.
Before the exemplary embodiments are described in more detail, it is to be understood that this disclosure is not limited to particular embodiments described, as such may, of course, vary. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present disclosure, exemplary methods and materials are now described.
As used herein and in the appended claims, the singular forms “a”, “an”, and “the” include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to “a gene” includes a plurality of such candidate agents and reference to “the cell” includes reference to one or more cells and equivalents thereof known to those skilled in the art, and so forth.
The publications discussed herein are provided solely for their disclosure prior to the filing date of the present application. Nothing herein is to be construed as an admission that the present disclosure is not entitled to antedate such publication by virtue of prior invention.
2. Parent Glucoamylases
In some embodiments, the present disclosure provides a glucoamylase variant. The glucoamylase variant is a variant of a parent glucoamylase, which may comprise both a catalytic domain and a starch binding domain. In some embodiments, the parent glucoamylase comprises a catalytic domain having an amino acid sequence as illustrated in SEQ ID NO: 1, 2, 3, 5, 6, 7, 8 or 9 or having an amino acid sequence displaying at least 80% sequence identity with one or more of the amino acid sequences illustrated in SEQ ID NO: 1, 2, 3, 5, 6, 7, 8, or 9. In yet other embodiments, the parent glucoamylase comprises a catalytic domain encoded by a DNA sequence that hybridizes under medium, high, or stringent conditions with a DNA encoding the catalytic domain of a glucoamylase having one of the amino acid sequences of SEQ ID NO: 1, 2 or 3.
In some embodiments, the parent glucoamylase comprises a starch binding domain having an amino acid sequence as illustrated in SEQ ID NO 1, 2, 161, 162, 163, 164, 165, 166, or 167, or having an amino acid sequence displaying at least 80% sequence identity with one or more of the amino acid sequence illustrated in SEQ ID NO: 1, 2, 161, 162, 163, 164, 165, 166, or 167. In yet other embodiments, the parent glucoamylase comprises a starch binding domain encoded by a DNA sequence that hybridizes under medium, high, or stringent conditions with a DNA encoding the starch binding domain of a glucoamylase having one of the amino acid sequences of SEQ ID NO: 1 or 2.
Predicted structure and known sequences of glucoamylases are conserved among fungal species (Coutinho et al., 1994, Protein Eng., 7:393-400 and Coutinho et al., 1994, Protein Eng., 7: 749-760). In some embodiments, the parent glucoamylase is a filamentous fungal glucoamylase. In some embodiments, the parent glucoamylase is obtained from a Trichoderma strain (e.g., T. reesei, T. longibrachiatum, T. strictipilis, T. asperellum, T. konilangbra and T. hazianum), an Aspergillus strain (e.g. A. niger, A. nidulans, A. kawachi, A. awamori and A. orzyae), a Talaromyces strain (e.g. T. emersonii, T. thermophilus, and T. duponti), a Hypocrea strain (e.g. H. gelatinosa, H. orientalis, H. vinosa, and H. citrina), a Fusarium strain (e.g., F. oxysporum, F. roseum, and F. venenatum), a Neurospora strain (e.g., N. crassa) and a Humicola strain (e.g., H. grisea, H. insolens and H. lanuginose), a Penicillium strain (e.g., P. notatum or P. chrysogenum), or a Saccharomycopsis strain (e.g., S. fibuligera).
In some embodiments, the parent glucoamylase may be a bacterial glucoamylase. For example, the polypeptide may be obtained from a gram-positive bacterial strain such as Bacillus (e.g., B. alkalophilus, B. amyloliquefaciens, B. lentos, B. licheniformis, B. stearothermophilus, B. subtilis and B. thuringiensis) or a Streptomyces strain (e.g., S. lividans).
In some embodiments, the parent glucoamylase will comprise a catalytic domain having at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 95% sequence identity, at least 97% sequence identity and also at least 98% sequence identity with the catalytic domain of the TrGA amino acid sequence of SEQ ID NO: 3.
In other embodiments, the parent glucoamylase will comprise a catalytic domain having at least 90% sequence identity, at least 93% sequence identity, at least 95% sequence identity, at least 96% sequence identity, at least 97% sequence identity, at least 98% sequence identity and also at least 99% sequence identity with the catalytic domain of the Aspergillus parent glucoamylase of SEQ ID NO: 5 or SEQ ID NO: 6.
In yet other embodiments, the parent glucoamylase will comprise a catalytic domain having at least 90% sequence identity, at least 95% sequence identity, at least 97% sequence identity and also at least 99% sequence identity with the catalytic domain of the Humicola grisea (HgGA) parent glucoamylase of SEQ ID NO: 8.
In some embodiments, the parent glucoamylase will comprise a starch binding domain having at least 80% sequence identity, at least 85% sequence identity, at least 90% sequence identity, at least 95% sequence identity, at least 97% sequence identity and also at least 98% sequence identity with the starch binding domain of the TrGA amino acid sequence of SEQ ID NO: 1, 2, or 161.
In other embodiments, the parent glucoamylase will comprise a starch binding domain having at least 90% sequence identity, at least 95% sequence identity, at least 97% sequence identity and also at least 99% sequence identity with the catalytic domain of the Humicola grisea (HgGA) glucoamylase of SEQ ID NO: 162.
In other embodiments, the parent glucoamylase will comprise a starch binding domain having at least 90% sequence identity, at least 95% sequence identity, at least 97% sequence identity and also at least 99% sequence identity with the catalytic domain of the Thielavia terrestris (TtGA) glucoamylase of SEQ ID NO: 163.
In other embodiments, the parent glucoamylase will comprise a starch binding domain having at least 90% sequence identity, at least 95% sequence identity, at least 97% sequence identity and also at least 99% sequence identity with the catalytic domain of the Thermomyces lanuginosus (ThGA) glucoamylase of SEQ ID NO: 164.
In other embodiments, the parent glucoamylase will comprise a starch binding domain having at least 90% sequence identity, at least 95% sequence identity, at least 97% sequence identity and also at least 99% sequence identity with the catalytic domain of the Talaromyces emersoniit (TeGA) glucoamylase of SEQ ID NO: 165.
In yet other embodiments, the parent glucoamylase will comprise a starch binding domain having at least 90% sequence identity, at least 93% sequence identity, at least 95% sequence identity, at least 96% sequence identity, at least 97% sequence identity, at least 98% sequence identity and also at least 99% sequence identity with the starch binding domain of the Aspergillus parent glucoamylase of SEQ ID NO: 166 or 167.
In some embodiments, the parent glucoamylase will have at least 80% sequence identity, at least 85% sequence identity, at least 88% sequence identity, at least 90% sequence identity, at least 93% sequence identity, at least 95% sequence identity, at least 96% sequence identity, at least 97% sequence identity, at least 98% sequence identity and also at least 99% sequence identity with the TrGA amino acid sequence of SEQ ID NO: 2.
In further embodiments, a Trichoderma glucoamylase homologue will be obtained from a Trichoderma or Hypocrea strain. Some typical Trichoderma glucoamylase homologues are described in U.S. Pat. No. 7,413,887 and reference is made specifically to amino acid sequences set forth in SEQ ID NOs: 17-22 and 43-47 of the reference.
In some embodiments, the parent glucoamylase is TrGA comprising the amino acid sequence of SEQ ID NO: 2, or a Trichoderma glucoamylase homologue having at least 80%, at least 85%, at least 88%, at least 90%, at least 93%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% sequence identity to the TrGA sequence (SEQ ID NO: 2).
A parent glucoamylase can be isolated and/or identified using standard recombinant DNA techniques. Any standard techniques can be used that are known to the skilled artisan. For example, probes and/or primers specific for conserved regions of the glucoamylase can be used to identify homologoues in bacterial or fungal cells (the catalytic domain, the active site, etc.). Alternatively, degenerate PCR can be used to identify homologues in bacterial or fungal cells. In some cases, known sequences, such as in a database, can be analyzed for sequence and/or structural identity to one of the known glucoamylases, including SEQ ID NO: 2, or a known starch binding domains, including SEQ ID NO: 161. Functional assays can also be used to identify glucoamylase activity in a bacterial or fungal cell. Proteins having glucoamylase activity can be isolated and reverse sequenced to isolate the corresponding DNA sequence. Such methods are known to the skilled artisan.
3. Glucoamylase Structural Homology
The central dogma of molecular biology is that the sequence of DNA encoding a gene for a particular enzyme, determines the amino acid sequence of the protein, this sequence in turn determines the three-dimensional folding of the enzyme. This folding brings together disparate residues that create a catalytic center and substrate binding surface and this results in the high specificity and activity of the enzymes in question.
Glucoamylases consist of as many as three distinct structural domains, a catalytic domain of approximately 450 residues that is structurally conserved in all glucoamylases, generally followed by a linker region consisting of between 30 and 80 residues that are connected to a starch binding domain of approximately 100 residues. The structure of the Trichoderma reesei glucoamylase with all three regions intact was determined to 1.8 Angstrom resolution herein (see Table 20 and Example 13). Using the coordinates (see Table 20) the structure was aligned with the coordinates of the catalytic domain from Aspergillus awamorii strain X100 that was determined previously (Aleshin, A. E., Hoffman, C., Firsov, L. M., and Honzatko, R. B. 1994 Refined crystal structures of glucoamylase from Aspergillus awamori var. X100. J Mol Biol 238: 575-591.). The Aspergillus awamori crystal structure only included the catalytic domain. As seen in FIGS. 12 and 13, the structure of the catalytic domains overlap very closely and it is possible to identify equivalent residues based on this structural superposition. It is believed that all glucoamylases share the basic structure depicted in FIGS. 12 and 13.
FIG. 12 is a comparison of the three dimensional structures of the Trichoderma glucoamylase (black) of SEQ ID NO: 1 (see FIG. 1 for amino acid sequence) and of Aspergillus awamorii (grey) viewed from the side. In this view the relationship between the catalytic domain and the linker region and the starch binding domain can be seen.
FIG. 13 is a comparison of the three dimensional structures of the Trichoderma glucoamylase (black) and of Aspergillus awamorii (grey) viewed from the top. The glucoamylases shown here and indeed all known glucoamylases to date share this structural homology. The conservation of structure correlates with the conservation of activity and a conserved mechanism of action for all glucoamylases. Given this high homology, changes resulting from site specific variants of the Trichoderma glucoamylase resulting in altered function would also have similar structural and therefore functional consequences in other glucoamylases. Therefore, the teachings of which variants result in desirable benefits can be applied to other glucoamylases.
A further crystal structure was produced using the coordinates in Table 20 for the Starch Binding Domain (SBD). The SBD for TrGA was aligned with the SBD for A. niger. As shown in FIG. 13, the structure of the A. niger and TrGA SBDs overlaps very closely. It is believed that while all starch binding domains share at least some of the basic structure depicted in FIG. 13, some SBDs are more structurally similar than others. For example, the TrGA SBD can be classified as within the carbohydrate binding module 20 family within the CAZY database (cazy.org). The CAZY database describes the families of structurally-related catalytic and carbohydrate-binding modules (or functional domains) of enzymes that degrade, modify, or create glycosidic bonds. Given a high structural homology, site specific variants of the TrGA SBD resulting in altered function would also have similar structural and therefore functional consequences in other glucoamylases having SBDs with similar structure to that of the TrGA SBD, particularly those classified within the carbohydrate binding module 20 family. Thus, the teachings of which variants result in desirable benefits can be applied to other SBDs having structural similarity.
Thus, the amino acid position numbers discussed herein refer to those assigned to the mature Trichoderma reesei glucoamylase sequence presented in FIG. 1. The present disclosure, however, is not limited to the variants of Trichoderma glucoamylase, but extends to glucoamylases containing amino acid residues at positions that are “equivalent” to the particular identified residues in Trichoderma reesei glucoamylase (SEQ ID NO: 2). In a embodiment of the present disclosure, the parent glucoamylase is Taleromyces glucoamylase and the substitutions are made at the equivalent amino acid residue positions in Taleromyces glucoamylase as those described herein. In other embodiments, the parent glucoamylase is one of those listed in Table 1. In further embodiments, the parent glucoamylase is a Penicillium glucoamylase, such as Penicillium chrysogenum, 
Structural identity determines whether the amino acid residues are equivalent. Structural identity is a one-to-one topological equivalent when the two structures (three dimensional and amino acid structures) are aligned. A residue (amino acid) position of a glucoamylase is equivalent to a residue of T. reesei glucoamylase if it is either homologous (i.e., corresponding in position in either primary or tertiary structure) or analogous to a specific residue or portion of that residue in T. reesei glucoamylase (having the same or similar functional capacity to combine, react, or interact chemically).
In order to establish identity to the primary structure, the amino acid sequence of a glucoamylase can be directly compared to Trichoderma reesei glucoamylase primary sequence and particularly to a set of residues known to be invariant in glucoamylases for which sequence is known. For example, FIGS. 4A and 4B herein shows the conserved residues between glucoamylases. FIGS. 4C and 4D show an alignment of starch binding domains from various glucoamylases. After aligning the conserved residues, allowing for necessary insertions and deletions in order to maintain alignment (i.e. avoiding the elimination of conserved residues through arbitrary deletion and insertion), the residues equivalent to particular amino acids in the primary sequence of Trichoderma reesei glucoamylase are defined. Alignment of conserved residues typically should conserve 100% of such residues. However, alignment of greater than 75% or as little as 50% of conserved residues is also adequate to define equivalent residues. Further, the structural identity can be used in combination with the sequence identity to identify equivalent residues.
For example, in FIGS. 4A and 4B, the catalytic domains of glucoamylases from six organisms are aligned to provide the maximum amount of homology between amino acid sequences. A comparison of these sequences shows that there are a number of conserved residues contained in each sequence as designated by an asterisk. These conserved residues, thus, may be used to define the corresponding equivalent amino acid residues of Trichoderma reesei glucoamylase in other glucoamylases such as glucoamylase from Aspergillus niger. Similarly, FIGS. 4C and 4D show the starch binding domains of glucoamylases from seven organisms aligned to identify equivalent residues.
Structural identity involves the identification of equivalent residues between the two structures. “Equivalent residues” can be defined by determining homology at the level of tertiary structure (structural identity) for an enzyme whose tertiary structure has been determined by X-ray crystallography. Equivalent residues are defined as those for which the atomic coordinates of two or more of the main chain atoms of a particular amino acid residue of the Trichoderma reesei glucoamylase (N on N, CA on CA, C on C and O on O) are within 0.13 nm and optionally 0.1 nm after alignment. Alignment is achieved after the best model has been oriented and positioned to give the maximum overlap of atomic coordinates of non-hydrogen protein atoms of the glucoamylase in question to the Trichoderma reesei glucoamylase. The best model is the crystallographic model giving the lowest R factor for experimental diffraction data at the highest resolution available.




  
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Equivalent residues that are functionally analogous to a specific residue of Trichoderma reesei glucoamylase are defined as those amino acids of the enzyme that may adopt a conformation such that they either alter, modify or contribute to protein structure, substrate binding or catalysis in a manner defined and attributed to a specific residue of the Trichoderma reesei glucoamylase. Further, they are those residues of the enzyme (for which a tertiary structure has been obtained by X-ray crystallography) that occupy an analogous position to the extent that, although the main chain atoms of the given residue may not satisfy the criteria of equivalence on the basis of occupying a homologous position, the atomic coordinates of at least two of the side chain atoms of the residue lie with 0.13 nm of the corresponding side chain atoms of Trichoderma reesei glucoamylase. The coordinates of the three dimensional structure of Trichoderma reesei glucoamylase are set forth in Table 15 and can be used as outlined above to determine equivalent residues on the level of tertiary structure.
Some of the residues identified for substitution are conserved residues whereas others are not. In the case of residues that are not conserved, the substitution of one or more amino acids is limited to substitutions that produce a variant that has an amino acid sequence that does not correspond to one found in nature. In the case of conserved residues, such substitutions should not result in a naturally-occurring sequence.
4. Variants
The variants according to the disclosure include at least one substitution, deletion or insertion in the amino acid sequence of a parent glucoamylase that makes the variant different in sequence from the parent glucoamylase. In some embodiments, the variants of the disclosure will have at least 20%, at least 40%, at least 60%, at least 80%, at least 85%, at least 90%, at least 95%, or at least 100% of the glucoamylase activity of the TrGA activity of SEQ ID NO: 2.
In some embodiments, the variants according to the disclosure will comprise a substitution, deletion, or insertion in at least one amino acid position of the parent TrGA (SEQ ID NO: 2), or in an equivalent position in the sequence of another parent glucoamylase having at least 80% sequence identity to the TrGA sequence, including but not limited to, at least 90%, at least 93%, at least 95%, at least 97%, and at least 99% sequence identity.
In other embodiments, the variant according to the disclosure will comprise a substitution, deletion or insertion in at least one amino acid position of a fragment of the parent TrGA, wherein the fragment comprises the catalytic domain of the TrGA sequence (SEQ ID NO: 3) or in an equivalent position in a fragment comprising the catalytic domain of a parent glucoamylase having at least 80% sequence identity to the fragment of the TrGA sequence, including but not limited to, at least 90%, at least 95%, at least 97%, and at least 99%. In some embodiments, the fragment will comprise at least 400, 425, 450, and/or 500 amino acid residues.
In other embodiments, the variant according to the disclosure will comprise a substitution, deletion or insertion in at least one amino acid position of a fragment of the parent TrGA, wherein the fragment comprises the starch binding domain of the TrGA sequence (SEQ ID NO: 161) or in an equivalent position in a fragment comprising the starch binding domain of a parent glucoamylase having at least 80% sequence identity to the fragment of the TrGA sequence, including but not limited to, at least 90%, at least 95%, at least 97%, and at least 99%. In some embodiments, the fragment will comprise at least 40, 50, 60, 70, 80, 90, 100, and/or 109 amino acid residues of TrGA starch binding domain (SEQ ID NO: 161).
In some embodiments, when the parent glucoamylase includes a catalytic domain, a linker region, and a starch binding domain, the variant will comprise a substitution, deletion or insertion in at least one amino acid position of a fragment comprising part of the linker region. In some embodiments, the variant will comprise a substitution deletion, or insertion in the amino acid sequence of a fragment of the TrGA sequence (SEQ ID NO: 2).
Structural identity with reference to an amino acid substitution means that the substitution occurs at the equivalent amino acid position in the homologous glucoamylase or parent glucoamylase. The term equivalent position means a position that is common to two parent sequences that is based on an alignment of the amino acid sequence of the parent glucoamylase in question as well as alignment of the three-dimensional structure of the parent glucoamylase in question with the TrGA reference glucoamylase amino acid sequence and three-dimensional sequence. For example, with reference to FIG. 5, position 24 in TrGA (SEQ ID NO: 2 or 3) is D24 and the equivalent position for Aspergillus niger (SEQ ID NO: 6) is position D25, and the equivalent position for Aspergillus oryzea (SEQ ID NO: 7) is position D26. See FIGS. 12 and 13 for an exemplary alignment of the three-dimensional sequence.
In some embodiments, the glucoamylase variant will include at least one substitution in the amino acid sequence of a parent. In further embodiments, the variant may have more than one substitution (e.g., two, three, or four substitutions).
In some embodiments, a glucoamylase variant comprises a substitution, deletion or insertion, and typically a substitution in at least one amino acid position in a position corresponding to the regions of non-conserved amino acids as illustrated in FIG. 5 (e.g., amino acid positions corresponding to those positions that are not designated by “*” in FIG. 5).
While the variants can be in any position in the mature protein sequence (SEQ ID NO: 2), in one embodiment, a glucoamylase variant comprises one or more substitutions in the following positions in the amino acid sequence set forth in SEQ ID NO: 2: 4, 5, 12, 24, 29, 43, 44, 45, 46, 47, 49, 51, 61, 70, 73, 75, 76, 94, 100, 108, 114, 116, 119, 122, 124, 125, 137, 143, 146, 148, 169, 171, 172, 175, 178, 180, 181, 208, 211, 228, 242, 243, 245, 292, 294, 297, 309, 310, 313, 314, 315, 316, 317, 321, 340, 341, 350, 353, 356, 363, 368, 369, 375, 376, 395, 398, 401, 408, 409, 412, 415, 417, 418, 421, 430, 431, 433, 436, 451, 503, 511, 535, 539, or 563; or in an equivalent position in a parent glucoamylase. In some embodiments, the parent glucoamylase will have at least 90%, at least 95%, at least 96%, at least 97% at least 98%, and at least 99% identity with SEQ ID NO: 2. In other embodiments the parent glucoamylase will be a Trichoderma glucoamylase homologue.
In some embodiments, the glucoamylase variant comprises one or more substitutions in the following positions in the amino acid sequence set forth in SEQ ID NO: 2:
D4, F5, I12, D24, F29, I43, D44, P45, D46, Y47, Y49, W51, N61, Y70, G73, Q75, R76, P94, D100, K108, K114, F116, N119, R122, Q124, R125, G137, N146, Q148, Y169, N171, Q172, F175, W178, E180, V181, Q208, S211, W228, N242, E243, R245, I292, G294, K297, R309, Y310, D313, V314, Y315, Y316, N317, W321, K340, K341, T350, Q356, T363, S368, S369, N376, Y395, A398, S401, R408, N409, T412, L417, H418, W421, T430, A431, R433, I436, S451, E503, Q511, A535, A539, or N563; or an equivalent position in parent glucoamylase (e.g., a Trichoderma glucoamylase homologue).
In other embodiments, the variant of a glucoamylase parent comprises one or more substitutions in the following positions in the amino acid sequence set forth in SEQ ID NO:2: 4, 5, 24, 29, 43, 44, 49, 61, 70, 73, 75, 76, 100, 108, 119, 124, 137, 146, 148, 169, 171, 172, 175, 178, 181, 208, 211, 243, 292, 294, 297, 314, 316, 317, 340, 341, 350, 356, 363, 368, 369, 376, 395, 401, 409, 412, 417, 430, 431, 433, 436, 451, 503, 511, 535, 539, or 563; or an equivalent position in a parent glucoamylase (e.g., a Trichoderma glucoamylase homologue).
In further embodiments, the variant of a glucoamylase parent comprises at least one of the following substitutions in the following positions in an amino acid sequence set forth in SEQ ID NO: 2: D4L/E/R/S/C/A/Q/W, F5C/M/N/R/S/T/V/W, I12L/R, D24E/L/Y/T, F29L/I/D/C/S/V/W, I43F/R/D/Y/S/Q, D44E/H/K/S/N/Y/F/R/C, Y47W, Y49N, N61D/I/L/Q/V/W, Q70R/K/M/P/G/L/F, G73F/C/L/W, Q75R/K/A, R76L/M/K/T/P, P94L, D100W/I/Q/M/P/A/N, N119P/T/Y/D/E, N146S/G/C/H/E/D/T/W/L/F/M, Q148V/Y/H/A/C/D/G/M/R/S/T, Y169D/F, Q172C/A/D/R/E/F/H/V/L/M/N/S/T/V, F175H/A/G/R/S/T/C/W/Y, W178A/C/D/E/F/G/H/K/N/R/S/T/V/Y, E180A/C/G/H/I/L/N/P/Q/R/S/T/V/Y/, V181E/C/D/G/H/I/P/T/Y/S/L/K/F/A, Q208L/A/C/E/N/F/H/T, S211C/R/E/A/Y/W/M/H/L/I/R/Q/T, E243S/R/N/M/Y/A/L, R245A/E/M/I/P/V, I292D/H/P/R/T/N/V/F/L, G294C/D/E/T/Q/I/A, K297F/L/P/T/M/D/N/Q/A/Y/H/S/R/W, R309A/C/G/H/I/N/P/Q/S/T/W/Y/L, Y310E/G/L/P/S/W/R/Q, D313Q, V314A/R/N/D/C/E/Q/G/H/I/L/K/M/F/P/S/T/W/Y, Y315F, Y316Q/R, N317T/H, K340D/T, K341F/D/P/V/G/S, T350S/E/A/N, Q356H/D/E, T363L/R/C/H/W, S368W/D/F/L, S369F, N376Q/T/H/S/V, Y395Q/R/S, A398S/I/T, S401C/V, R408S, N409W/T/K, T412A/H/K/G, L417A/D/E/F/G/I/K/Q/R/S/T/V/W/Y, T430A/E/F/G/H/I/K/M/N/Q/R/V, A431C/E/H/I/L/M/Q/R/S/W/Y, R433H/Q, I436A/T, S451M/T/H, E503A/C/D/H/S/V/W, Q511C/G/H/I/K/T/V, A535E/F/G/K/L/N/P/R/S/T/V/W/Y, A539E/H/M/R/S/W, or N563/A/C/E/I/K/L/Q/TN; or a substitution in an equivalent position in a parent glucoamylase homologue.
In some embodiments, the glucoamylase variant comprises at least one substitution in a position corresponding to the amino acid residue position set forth in SEQ ID NO: 2: 5, 24, 43, 44, 49, 61, 70, 73, 75, 76, 94, 119, 146, 148, 172, 175, 178, 180, 181, 208, 211, 245, 294, 353, 315, 375, 409, 309, 314, 369, 412, 417, 430, 431, 503, 511, 535, 539, or 563; or an equivalent position in a homologous parent glucoamylase.
In some representative embodiments, the glucoamylase variant comprises at least one substitution selected from the group consisting of F5W, D24E, I43R, I43Y, I43Q, I435, I43F, D44C, D44R, Y47W, Y49N, N61I, Q70K, G73F, Q75R, R76L, P94L, N119P/T/Y/D, N146S/D/T/E/W/L, Q148V N171D, Q172C/D/R/E/F/V/L/T, F175R/W/Y, W178K/N/Y, E180H/N/V/R, V181E/F/G/I/H, Q208A/T/N, S211H/M/L/R, R245E, R245M, G294C, R309W, V314F/G/H/K/P/R/Y, Y315F, S369F, T412K, L417R, L417V, T430A, T430M, A431L, A431Q, E503A, E503V, Q511H, A535R, A539R, N563I, and N563K corresponding to the position set forth in SEQ ID NO: 2, or an equivalent position in a homologous parent glucoamylase.
In further particular embodiments, the glucoamylase variant comprises at least one substitution of an amino acid residue selected from the positions corresponding to position 5, 43, 44, 61, 73, 75, 76, 94, 108, 119, 124, 146, 148, 171, 172, 175, 178, 180, 181, 208, 211, 294, 297, 314, 316, 412, 417, 430, 431, 503, 511, 535, 539, or 563 of SEQ ID NO: 2, or an equivalent position in a Trichoderma glucoamylase homologue. In some embodiments, the substitution is at a position corresponding to position number 43, 44, 61, 73, 148, 172, 175, 178, 180, 208, 211, 294, 297, 314, 412, 417, 430, 431, 503, 511, 535, 539, or 563 of SEQ ID NO: 2, or an equivalent position in a Trichoderma glucoamylase homologue.
In some representative embodiments, the substitution is at a position corresponding to position number 43, 44, 61, 73, 108, 124, 171, 172, 208, 211, 294, 314, 316, 417, 430, 431, 503, 511, 535, 539, or 563 of SEQ ID NO: 2, or a homologous parent glucoamylase (e.g., Trichoderma glucoamylase homologue).
In some embodiments, the glucoamylase variants comprise multiple substitutions. Some of the multiple substitutions will include a substitution at one or more of the positions equivalent to and including the positions 24, 43, 44, 108, 124, 171, 175, 181, 208, 243, 292, 294, 297, 310, 314, 363, 417, 430, 431, 503, 511, 535, 539, or 563 of SEQ ID NO: 2. Some typical multiple substitutions will include one or more of the positions equivalent to and corresponding to positions 43, 44, 61, 73, 08, 124, 171, 208, 211, 294, 314 417, 430, 431, 503, 511, 535, 539, or 563 of SEQ ID NO: 2.
Some examples of variants with multiple substitutions include substitutions at positions:
D24/I43/D44/F175/V181/V314/T363;
D24/Q208/I292/G294/K297/Y310;
V181/E243/I292/k297/N317/Y395;
D24/V181/Q208/G294/T363/N376/N409;
D24/V181/I292/G294/E243/N409;
I43R/E243/I292/G294/K297;
I43/D44/N61/L417/E503/Q511/A539;
I43/D44/L417/E503/Q511/A539;
I43/N61/L417/T430/Q511/A539;
I43/N61/L417/E503/Q511/A539;
I43/N61/T430/A431/Q511/A539;
I43/N61/T430/Q511;
I43/N61/T430/Q511/A539;
I43/N61/Q511;
I43/N61/Q511/A539;
I43/G73/T430;
I43/L417/E503/Q511/A539;
I43/L417/Q511;
I43/L417/T430/A431/Q511/A539;
I43/L417/T430/Q511;
I43/L417/T430/Q511/A539;
I43/L417/E503/A539;
I43/L417/E503/Q511/A539;
I43/T430;
I43/T430/A431/E503/Q511;
I43/T430/A431/Q511;
I43/T430/A431/Q511/A539;
I43/T430/E503/Q511;
I43/T430/Q511;
I43Q/T430/Q511/A539;
I43/A431/Q511;
I43/T430/E503/Q511/N563;
I43/T430/E503/A535/N563;
I43/E503/Q511/A539;
I43/Q511/A539;
D44/G73/L417/N563;
D44/G73/E503/Q511;
D44/G73/N563;
D44/L417/N563;
D44/T430/Q511/A535;
D44/E503/Q511/N563;
G73/T430/E503/Q511;
G73/T430/Q511;
G294/L417/A431;
G294/L417/A431;
G294/L417/A431/Q511;
L417/T430/A431/Q511/A535/A539/N563;
L417/A431/Q511;
L417/T430/Q511/A535/N563;
L417/T430/Q511/A539/N563; and E503/N563;
of SEQ ID NO: 2, or equivalent positions in parent glucoamylases and particularly Trichoderma glucoamylase homologues.
Some variants with multiple substitutions may include the substitutions at positions:
Y47F/W, Y315F/W;
D24E,L/I43F,R/D44H,N/F175H/V181K,L/V314D,H,K/T363R;
D24L,W,Y/Q208F/I292F,N,V/G294A,I,Q/K297A/Y310F,Q,R;
V181F,K,L/E243A,N,M,R,Y/I292F,L,N,V/K297A,D,H,M,N,Q/N317H/Y395Q,R;
D24E,L,Y/V181F,K,L/Q208C,F/G294A,I,Q/T363R/N376Q/N409K,W;
D24E,L,Y/V181F,K,L/I292F,L,N,V/G294A,I,Q/E243A,M,N,R,Y/N409K,W;
I43R/E243A,M,N,R,Y/I292F,L,N,V/G294A/K297A,D,H,M,N,Q,S,R,W,Y;
I43Q/D44C/N61I/L417V/E503A/Q511H/A539R;
I43Q/D44C/L417V/E503A/Q511H/A539R;
I43Q/N61I/L417V/E503A/Q511H/A539R;
I43Q/N61I/L417V/T430M/Q511H/A539R;
I43R/N61I/L417R,V/E503A/Q511H/A539R;
I43Q/N61I/T430A/A431L/Q511H/A539R;
I43Q/N61I/T430A/Q511H;
I43Q/N61I/T430A/Q511H/A539R;
I43Q/N61I/T430M/Q511H/A539R I43Q/N61I/Q511H;
I43Q/N61I/Q511H/A539R;
I43R/G73F/T430A;
I43Q/L417V/T430A/A431L/Q511H/A539R;
I43Q/L417V/T430A/Q511H;
I43Q/L417V/T430A/Q511H/A539R;
I43R/L417R/E503A/A539R;
I43R,Q/L417V/E503A/Q511H/A539R;
I43Q/L417V/Q511H;
I43R,Q/T430A;
I43Q/T430A/A431L/E503A/Q511H;
I43Q/T430A/A431L/Q511H;
I43Q/T430A/A431L/Q511H/A539R;
I43Q/T430A/E503A/Q511H;
I43Q/T430A/Q511H;
I43Q/T430A,M/Q511H/A539R;
I43R/T430A/E503A,V/Q511H/N563K;
I43Q/A431L/Q511H;
I43Q/E503A/Q511H/A539R;
I43Q/Q511H/A539R;
D44C/G73F/E503V/Q511H;
D44C/G73F/L417R/N563K;
D44C/G73F/N563K;
D44C/L417R/N563K;
D44R/E503A/Q511H/N563I;
D44R/T430A/Q511H/A535R;
G73F/T430A/E503V/Q511H;
G73F/T430A/Q511H;
G294C/L417R/A431L;
G294C/L417R/A431L,Q/Q511H;
G294C/L417V/A431Q;
L417R,V/A431L,Q/Q511H;
L417V/T430A/A431L,Q/Q511H/A535R/A539R/N563I;
L417V/T430A/Q511H/A535R/N563I;
L417V/T430A/Q511H/A539R/N563I; and
E503A/N563I
of SEQ ID NO: 2, or equivalent positions in parent glucoamylases and particularly Trichoderma glucoamylase homologue.
A number of parent glucoamylases have been aligned with the amino acid sequence of TrGA. FIGS. 4A and 4B include the catalytic domain of the following parent glucoamylases: Aspergillus awamori (AaGA) (SEQ ID NO: 5); Aspergillus niger (AnGA) (SEQ ID NO: 6); Aspergillus orzyae (AoGA) (SEQ ID NO: 7), Humicola grisea (HgGA) (SEQ ID NO: 8) and Hypocrea vinosa (HvGA) (SEQ ID NO: 9). The percent identity of the catalytic domains is represented in Table 1 below. FIGS. 4C and 4D include the starch binding domain of the following parent glucoamylases: Trichoderma reesei (TrGA) (SEQ ID NO: 161), Humicola grisea (HgGA) (SEQ ID NO: 162), Thielavia terrestris (TtGA) (SEQ ID NO: 163), Thermomyces lanuginosus (ThGA) (SEQ ID NO: 164), Talaromyces emersonii (TeGA) (SEQ ID NO: 165), Aspergillus niger (AnGA) (SEQ ID NO: 166), and Aspergillus awamori (AaGA) (SEQ ID NO: 167). In some embodiments, for example, the variant glucoamylase will be derived from a parent glucoamylase that is an Aspergillus glucoamylase and the variant will include at least one substitution in a position equivalent to a position set forth in SEQ ID NO: 2, and particularly in a position corresponding to D4, F5, I12, D24, F29, I43, D44, P45, D46, Y47, Y49, W51, N61, Y70, G73, Q75, R76, P94, D100, K108, K114, F116, N119, R122, Q124, R125, G137, N146, Q148, Y169, N171, Q172, F175, W178, E180, V181, Q208, S211, W228, N242, E243, R245, I292, G294, K297, R309, Y310, D313, V314, Y315, Y316, N317, W321, K340, K341, T350, Q356, T363, S368, S369, N376, Y395, A398, S401, R408, N409, T412, L417, H418, W421, T430, A431, R433, I436, S451, E503, Q511, A535, A539, or N563.
Endo-H removal of N-linked sugars in the Trichoderma reesei glucoamylase had a stabilizing effect (when looking at Tm). Thus, variants having an N171D substitution can have increased thermostability as compared to the wild-type parent. In some embodiments, variants having one or more substitutions at sites having N-linked sugars are provided, including N171D in Trichoderma reesei (SEQ ID NO: 2).














TABLE 1







AaGA
AnGA
AoGA
HgGA
HvGA
TrGA






















AaGA
100
95
58
53
57
56


AnGA

100
59
53
57
56


AoGA


100
55
56
56


HgGA



100
61
63


HvGA




100
91


TrGA





100









The present disclosure also provides glucoamylase variants having at least one altered property (e.g., improved property) as compared to a parent glucoamylase and particularly to the TrGA. In some embodiments, at least one altered property (e.g., improved property) is selected from the group consisting of acid stability, thermal stability and specific activity. Typically, the altered property is increased acid stability, increased thermal stability and/or increased specific activity. The increased thermal stability typically is at higher temperatures. In one embodiment, the increased pH stability is at high pH. In a further embodiment, the increased pH stability is at low pH.
The glucoamylase variants of the disclosure may also provide higher rates of starch hydrolysis at low substrate concentrations as compared to the parent glucoamylase. The variant may have a higher Vmax or lower Km than a parent glucoamylase when tested under the same conditions. For example the variant glucoamylase may have a higher Vmax at a temperature range of 25° C. to 70° C. (e.g., at 25° C. to 35° C.; 30° C. to 35° C.; 40° C. to 50° C.; at 50° C. to 55° C. and at 55° C. to 62° C.). The Michaelis-Menten constant, Km and Vmax values can be easily determined using standard known procedures.
5. Thermal Stability
Thermostable Variants
In one aspect, the disclosure relates to a variant glucoamylase having altered thermal stability at altered temperatures as compared to a parent or wild type. Altered temperatures include increased or decreased temperatures. In some embodiments, the glucoamylase variant will have improved thermostability such as retaining at least 50%, 60%, 70%, 75%, 80%, 85%, 90%, 92%, 95%, 96%, 97%, 98% or 99% enzymatic activity after exposure to altered temperatures over a given time period, for example, at least 60 minutes, 120 minutes, 180 minutes, 240 minutes, 300 minutes, etc. In some embodiments, the variant has increased thermal stability compared to the parent glucoamylase at selected temperatures in the range of 40 to 80° C., also in the range of 50 to 75° C. and in the range of 60 to 70° C., and typically at a pH range of 4.0 to 6.0. In some embodiments, the thermostability is determined as described in the Examples.
In some embodiments, particularly interesting variants in connection with an improvement in thermostability include one or more deletions, substitutions or insertions and particularly substitutions in the following positions in the amino acid sequence set forth in SEQ ID NO: 2: D4, F5, I12, D24, F29, I43, D44, P45, D46, Y47, Y49, W51, N61, Y70, G73, Q75, R76, P94, D100, K108, K114, F116, N119, R122, Q124, R125, G137, N146, Q148, Y169, N171, Q172, F175, W178, E180, V181, Q208, S211, W228, N242, E243, R245, I292, G294, K297, R309, Y310, D313, V314, Y315, Y316, N317, W321, K340, K341, T350, Q356, T363, S368, S369, N376, Y395, A398, S401, R408, N409, T412, L417, H418, W421, T430, A431, R433, I436, S451, E503, Q511, A535, A539, or N563; or an equivalent position in a parent glucoamylase. In some embodiments, the parent glucoamylase will be a Trichoderma glucoamylase homologue, and in typical embodiments, the parent glucoamylase will have at least 90%, at least 95%, or at least 98% sequence identity to SEQ ID NO: 2.
6. Chimeric Glucoamylases
Glucoamylase variants of the disclosure may also include chimeric or hybrid glucoamylases with, for example a starch binding domain (SBD) from one glucoamylase and a catalytic domain and linker from another. For example, a hybrid glucoamylase can be made by swapping the SBD from AnGA with the SBD from TrGA, making a hybrid with the AnGA SBD and the TrGA catalytic domain and linker. Alternatively, the SBD and linker from AnGA can be swapped for the SBD and linker of TrGA.
7. Specific Activity
In another aspect, the disclosure relates to a variant glucoamylase having altered specific activity as compared to a parent or wild-type glucoamylase.
In some embodiments, particularly interesting variants in connection with an improvement in specific activity include one or more deletions, substitutions or insertions and particularly substitutions in the following positions in the amino acid sequence set forth in SEQ ID NO: 2: D4, F5, I12, D24, F29, I43, D44, P45, D46, Y47, Y49, W51, N61, Y70, G73, Q75, R76, P94, D100, K108, K114, F116, N119, R122, Q124, R125, G137, N146, Q148, Y169, N171, Q172, F175, W178, E180, V181, Q208, S211, W228, N242, E243, R245, I292, G294, K297, R309, Y310, D313, V314, Y315, Y316, N317, W321, K340, K341, T350, Q356, T363, S368, S369, N376, Y395, A398, S401, R408, N409, T412, L417, T430, A431, H418, W421, R433, I436, S451, E503, Q511, A535, A539, or N563; or an equivalent position in a parent glucoamylase. In some embodiments, variants of the disclosure having improved specific activity include a substitution in the following positions in the amino acid sequence set forth in SEQ ID NO: 2: D4, D24, I43, D44, N61, Y70, G73, Q75, R76, D100, K108, N119, Q124, N146, Q148, N171, Q172, F175, V181, Q208, S211, E243, R245, I292, G294, K297, V314, Y316, N317, K340, K341, T350, Q356, T363, S368, N376, Y395, A398, S401, N409, T412, L417, T430, A431, I436, S451, E503, Q511, A535, A539, or N563; or an equivalent position in a parent glucoamylase. In some embodiments, the parent glucoamylase will comprise a sequence having at least 90% or 95% sequence identity to the sequence of SEQ ID NO: 2.
8. Polynucleotides
The present disclosure also relates to isolated polynucleotides encoding a variant glucoamylase of the disclosure. The polynucleotides encoding a variant glucoamylase may be prepared by established techniques known in the art. The polynucleotides may be prepared synthetically, such as by an automatic DNA synthesizer. The DNA sequence may be of mixed genomic (or cDNA) and synthetic origin prepared by ligating fragments together. The polynucleotides may also be prepared by polymerase chain reaction (PCR) using specific primers. In general, reference is made to Minshull J., et al., (2004), Engineered protein function by selective amino acid diversification, Methods 32(4):416-427. Also a number of companies now synthesize DNA such as Geneart AG, Regensburg, Germany.
The present disclosure also provides isolated polynucleotides comprising a nucleotide sequence (i) having at least 70% identity to SEQ ID NO: 4, or (ii) being capable of hybridizing to a probe derived from the nucleotide sequence set forth in SEQ ID NO: 4, under conditions of intermediate to high stringency, or (iii) being complementary to a nucleotide sequence having at least 90% sequence identity to the sequence set forth in SEQ ID NO: 4. Probes useful according to the disclosure may include at least 50, 100, 150, 200, 250, 300 or more contiguous nucleotides of SEQ ID NO: 4.
The present disclosure further provides isolated polynucleotides that encode variant glucoamylases that comprise an amino acid sequence comprising at least 80% amino acid sequence identity to SEQ ID NO: 2. In some embodiments, the variant glucoamylases have at least 80% amino acid sequence identity to SEQ ID NO: 2. In some embodiments, the variant glucoamylases have at least 90% amino acid sequence identity to SEQ ID NO: 2. In some embodiments, the variant glucoamylases have at least 93% amino acid sequence identity to SEQ ID NO: 2. In some embodiments, the variant glucoamylases have at least 95% amino acid sequence identity to SEQ ID NO: 2. In some embodiments, the variant glucoamylases have at least 97% amino acid sequence identity to SEQ ID NO: 2. In some embodiments, the variant glucoamylases have at least 98% amino acid sequence identity to SEQ ID NO: 2. In some embodiments, the variant glucoamylases have at least 99% amino acid sequence identity to SEQ ID NO: 2. The present disclosure also provides expression vectors comprising any of the polynucleotides provided above.
The present disclosure also provides fragments (i.e., portions) of the DNA encoding the variant glucoamylases provided herein. These fragments find use in obtaining partial length DNA fragments capable of being used to isolate or identify polynucleotides encoding mature glucoamylase enzymes described herein from filamentous fungal cells (e.g., Trichoderma, Aspergillus, Fusarium, Penicillium, Schizosaccharomyces, and Humicola), or a segment thereof having glucoamylase activity. In some embodiments, fragments of the DNA may comprise at least 50, 100, 150, 200, 250 300 or more contiguous nucleotides. In some embodiments, portions of the DNA provided in SEQ ID NO: 4 may be used in obtaining parent glucoamylase and particularly Trichoderma glucoamylase homologues from other species, such as filamentous fungi that encode a glucoamylase.
9. DNA Constructs and Vectors
According to one embodiment of the disclosure, a DNA construct comprising a polynucleotide as described above encoding a variant glucoamylase encompassed by the disclosure and operably linked to a promoter sequence is assembled to transfer into a host cell.
The DNA construct may be introduced into a host cell using a vector. The vector may be any vector that when introduced into a host cell is typically integrated into the host cell genome and is replicated. Vectors include cloning vectors, expression vectors, shuttle vectors, plasmids, phage particles, cassettes and the like. In some embodiments, the vector is an expression vector that comprises regulatory sequences operably linked to the glucoamylase coding sequence.
Examples of suitable expression and/or integration vectors are provided in Sambrook et al., (1989) supra, and Ausubel (1987) supra, and van den Hondel et al. (1991) in Bennett and Lasure (Eds.) MORE GENE MANIPULATIONS IN FUNGI, Academic Press pp. 396-428 and U.S. Pat. No. 5,874,276. Fungal Genetics Stock Center Catalogue of Strains (FGSC, <www.fgsc.net>) also discloses useful vectors. Particularly useful vectors include vectors obtained from, for example, Invitrogen and Promega.
Specific vectors suitable for use in fungal host cells include vectors such as pFB6, pBR322, pUC18, pUC100, pDONRTM201, pDONRTM221, pENTRTM, pGEM®3Z and pGEM®4Z. A general purpose expression vector useful in Aspergillus includes pRAX with a glaA promoter, and in Hypocrea/Trichoderma includes pTrex3g with a cbh1 promoter.
Suitable plasmids for use in bacterial cells include pBR322 and pUC19 permitting replication in E. coli and pE194 for example permitting replication in Bacillus. 
In some embodiments, the promoter shows transcriptional activity in a bacterial or a fungal host cell and may be derived from genes encoding proteins either homologous or heterologous to the host cell. The promoter may be a mutant, a truncated and/or a hybrid promoter. The above-mentioned promoters are known in the art.
Examples of suitable promoters useful in fungal cells and particularly filamentous fungal cells such as Trichoderma or Aspergillus cells include such exemplary promoters as the T. reesei promoters cbh1, cbh2, egl1, egl2, eg5, xln1 and xln2. Other examples of useful promoters include promoters from A. awamori and A. niger glucoamylase genes (glaA) (see, Nunberg et al., (1984) Mol. Cell. Biol. 4:2306-2315 and Boel et al., (1984) EMBO J. 3:1581-1585), A. oryzae TAKA amylase promoter, the TPI (triose phosphate isomerase) promoter from S. cerevisiae, the promoter from Aspergillus nidulans acetamidase genes and Rhizomucor miehei lipase genes.
Examples of suitable promoters useful in bacterial cells include those obtained from the E. coli lac operon; Bacillus licheniformis alpha amylase gene (amyL), B. stearothermophilus amylase gene (amyM); Bacillus subtilis xylA and xylB genes, the beta-lactamase gene, and the tac promoter.
In one embodiment, the promoter is one that is native to the host cell. For example, when T. reesei is the host, the promoter is a native T. reesei promoter. In another embodiment, the promoter is one that is heterologous to the fungal host cell. In some embodiments the promoter will be the parent glucoamylase promoter such as the TrGA promoter.
In some embodiments, the DNA construct includes nucleic acids coding for a signal sequence that is an amino acid sequence linked to the amino terminus of the polypeptide that directs the encoded polypeptide into the cell's secretory pathway. The 5′ end of the coding sequence of the nucleic acid sequence may naturally include a signal peptide coding region that is naturally linked in translation reading frame with the segment of the glucoamylase coding sequence that encodes the secreted glucoamylase or the 5′ end of the coding sequence of the nucleic acid sequence may include a signal peptide that is foreign to the coding sequence. In some embodiments, the DNA construct includes a signal sequence that is naturally associated with a parent glucoamylase gene from that a variant glucoamylase has been obtained. In some embodiments the signal sequence will be the sequence depicted in SEQ ID NO: 1 or a sequence having at least 90%, at least 94% and at least 98% sequence identity thereto. Effective signal sequences may include the signal sequences obtained from glucoamylases of other filamentous fungal enzymes, such as from Trichoderma (T. reesei glucoamylase), Humicola (H. insolens cellulase or H. grisea glucoamylase), Aspergillus (A. niger glucoamylase and A. oryzae TAKA amylase), and Rhizopus. 
In additional embodiments, a DNA construct or vector comprising a signal sequence and a promoter sequence to be introduced into a host cell are derived from the same source. In some embodiments, the native glucoamylase signal sequence of a Trichoderma glucoamylase homologue, such as a signal sequence from a Hypocrea strain may be used.
In some embodiments, the expression vector also includes a termination sequence. Any terminator sequence functional in the host cell may be used in the present disclosure. In one embodiment, the termination sequence and the promoter sequence are derived from the same source. In another embodiment, the termination sequence is homologous to the host cell. Useful terminator sequence include terminator sequences obtained from the genes of Trichoderma reesei cbh1; A. niger or A. awamori glucoamylase (Nunberg et al. (1984) supra, and Boel et al., (1984) supra), Aspergillus nidulans anthranilate synthase, Aspergillus oryzae TAKA amylase, or A. nidulans trpC (Punt et al., (1987) Gene 56:117-124).
In some embodiments, an expression vector includes a selectable marker. Examples of typical selectable markers include ones that confer antimicrobial resistance (e.g., hygromycin and phleomycin). Nutritional selective markers also find use in the present disclosure including those markers known in the art as amdS (acetamidase), argB (ornithine carbamoyltransferase) and pyrG (orotidine-5′ phosphate decarboxylase). Markers useful in vector systems for transformation of Trichoderma are known in the art (see, e.g., Finkelstein, chapter 6 in BIOTECHNOLOGY OF FILAMENTOUS FUNGI, Finkelstein et al. Eds. Butterworth-Heinemann, Boston, Mass. (1992); Kinghorn et al. (1992) APPLIED MOLECULAR GENETICS OF FILAMENTOUS FUNGI, Blackie Academic and Professional, Chapman and Hall, London; Berges and Barreau (1991) Curr. Genet. 19:359-365; and van Hartingsveldt et al., (1987) Mol. Gen. Genet. 206:71-75). In a typical embodiment, the selective marker is the amdS gene, which encodes the enzyme acetamidase, allowing transformed cells to grow on acetamide as a nitrogen source. The use of A. nidulans amdS gene as a selective marker is described in Kelley et al., (1985) EMBO J. 4:475-479 and Penttilä et al., (1987) Gene 61:155-164.
Methods used to ligate the DNA construct comprising a nucleic acid sequence encoding a variant glucoamylase, a promoter, a terminator and other sequences and to insert them into a suitable vector are well known in the art. Linking is generally accomplished by ligation at convenient restriction sites. If such sites do not exist, synthetic oligonucleotide linkers are used in accordance with conventional practice. (See, Sambrook (1989) supra, and Bennett and Lasure, MORE GENE MANIPULATIONS IN FUNGI, Academic Press, San Diego (1991) pp 70-76.). Additionally, vectors can be constructed using known recombination techniques (e.g., Invitrogen Life Technologies, Gateway Technology).
10. Host Cells
The present disclosure also relates to host cells comprising a polynucleotide encoding a variant glucoamylase of the disclosure, which are used to produce the glucoamylases of the disclosure. In some embodiments, the host cells are selected from bacterial, fungal, plant and yeast cells. The term host cell includes both the cells, progeny of the cells and protoplasts created from the cells that are used to produce a variant glucoamylase according to the disclosure.
In some embodiments, the host cells are fungal cells and typically filamentous fungal host cells. The term “filamentous fungi” refers to all filamentous forms of the subdivision Eumycotina (See, Alexopoulos, C. J. (1962), INTRODUCTORY MYCOLOGY, Wiley, New York). These fungi are characterized by a vegetative mycelium with a cell wall composed of chitin, cellulose, and other complex polysaccharides. The filamentous fungi of the present disclosure are morphologically, physiologically, and genetically distinct from yeasts. Vegetative growth by filamentous fungi is by hyphal elongation and carbon catabolism is obligatory aerobic. In the present disclosure, the filamentous fungal parent cell may be a cell of a species of, but not limited to, Trichoderma, (e.g., Trichoderma reesei, the asexual morph of Hypocrea jecorina, previously classified as T. longibrachiatum, Trichoderma viride, Trichoderma koningii, Trichoderma harzianum) (Sheir-Neirs et al., (1984) Appl. Microbiol. Biotechnol 20:46-53; ATCC No. 56765 and ATCC No. 26921); Penicillium sp., Humicola sp. (e.g., H. insolens, H. lanuginosa and H. grisea); Chrysosporium sp. (e.g., C. lucknowense), Gliocladium sp., Aspergillus sp. (e.g., A. oryzae, A. niger, A. sojae, A. japonicus, A. nidulans, and A. awamori) (Ward et al., (1993) Appl. Microbiol. Biotechnol. 39:738-743 and Goedegebuur et al., (2002) Genet. 41:89-98), Fusarium sp., (e.g., F. roseum, F. graminum F. cerealis, F. oxysporuim and F. venenatum), Neurospora sp., (N. crassa), Hypocrea sp., Mucor sp., (M. miehei), Rhizopus sp. and Emericella sp. (see also, Innis et al., (1985) Sci. 228:21-26). The term “Trichoderma” or “Trichoderma sp.” or “Trichoderma spp.” refer to any fungal genus previously or currently classified as Trichoderma. 
In some embodiments, the host cells will be gram-positive bacterial cells. Non-limiting examples include strains of Streptomyces, (e.g., S. lividans, S. coelicolor and S. griseus) and Bacillus. As used herein, “the genus Bacillus” includes all species within the genus “Bacillus,” as known to those of skill in the art, including but not limited to B. subtilis, B. licheniformis, B. lentus, B. brevis, B. stearothermophilus, B. alkalophilus, B. amyloliquefaciens, B. clausii, B. halodurans, B. megaterium, B. coagulans, B. circulans, B. lautus, and B. thuringiensis. It is recognized that the genus Bacillus continues to undergo taxonomical reorganization. Thus, it is intended that the genus include species that have been reclassified, including but not limited to such organisms as B. stearothermophilus, which is now named “Geobacillus stearothermophilus.”
In some embodiments the host cell is a gram-negative bacterial strain, such as E. coli or Pseudomonas sp. In other embodiments, the host cells may be yeast cells such as Saccharomyces sp., Schizosaccharomyces sp., Pichia sp., or Candida sp.
In other embodiments, the host cell will be a genetically engineered host cell wherein native genes have been inactivated, for example by deletion in bacterial or fungal cells. Where it is desired to obtain a fungal host cell having one or more inactivated genes known methods may be used (e.g. methods disclosed in U.S. Pat. No. 5,246,853, U.S. Pat. No. 5,475,101, and WO 92/06209). Gene inactivation may be accomplished by complete or partial deletion, by insertional inactivation or by any other means that renders a gene nonfunctional for its intended purpose (such that the gene is prevented from expression of a functional protein). In some embodiments, when the host cell is a Trichoderma cell and particularly a T. reesei host cell, the cbh1, cbh2, egl1 and egl2 genes will be inactivated and/or typically deleted. Typically, Trichoderma reesei host cells having quad-deleted proteins are set forth and described in U.S. Pat. No. 5,847,276 and WO 05/001036. In other embodiments, the host cell is a protease deficient or protease minus strain.
11. Transformation of Host Cells
Introduction of a DNA construct or vector into a host cell includes techniques such as transformation; electroporation; nuclear microinjection; transduction; transfection, (e.g., lipofection mediated and DEAE-Dextrin mediated transfection); incubation with calcium phosphate DNA precipitate; high velocity bombardment with DNA-coated microprojectiles; and protoplast fusion. General transformation techniques are known in the art (See, e.g., Ausubel et al., (1987), supra, chapter 9; and Sambrook (1989) supra, and Campbell et al., (1989) Curr. Genet. 16:53-56).
Transformation methods for Bacillus are disclosed in numerous references including Anagnostopoulos C and J. Spizizen (1961) J. Bacteriol. 81:741-746 and WO 02/14490.
Transformation methods for Aspergillus are described in Yelton et al (1984) Proc. Natl. Acad. Sci. USA 81:1470-1474; Berka et al., (1991) in Applications of Enzyme Biotechnology, Eds. Kelly and Baldwin, Plenum Press (NY); Cao et al., (2000) Science 9:991-1001; Campbell et al., (1989) Curr. Genet. 16:53-56 and EP 238 023. The expression of heterologous protein in Trichoderma is described in U.S. Pat. No. 6,022,725; U.S. Pat. No. 6,268,328; Harkki et al. (1991); Enzyme Microb. Technol. 13:227-233; Harkki et al., (1989) Bio Technol. 7:596-603; EP 244,234; EP 215,594; and Nevalainen et al., “The Molecular Biology of Trichoderma and its Application to the Expression of Both Homologous and Heterologous Genes”, in MOLECULAR INDUSTRIAL MYCOLOGY, Eds. Leong and Berka, Marcel Dekker Inc., NY (1992) pp. 129-148). Reference is also made to WO96/00787 and Bajar et al., (1991) Proc. Natl. Acad. Sci. USA 88:8202-8212 for transformation of Fusarium strains.
In one specific embodiment, the preparation of Trichoderma sp. for transformation involves the preparation of protoplasts from fungal mycelia (See, Campbell et al., (1989) Curr. Genet. 16:53-56; Pentilla et al., (1987) Gene 61:155-164). Agrobacterium tumefaciens-mediated transformation of filamentous fungi is known (See, de Groot et al., (1998) Nat. Biotechnol. 16:839-842). Reference is also made to U.S. Pat. No. 6,022,725 and U.S. Pat. No. 6,268,328 for transformation procedures used with filamentous fungal hosts.
Typically, genetically stable transformants are constructed with vector systems whereby the nucleic acid encoding the variant glucoamylase is stably integrated into a host strain chromosome. Transformants are then purified by known techniques.
In some further embodiments, the host cells are plant cells, such as cells from a monocot plant (e.g. corn, wheat and sorghum) or cells from a dicot plant (e.g. soybean). Methods for making DNA constructs useful in transformation of plants and methods for plant transformation are known. Some of these methods include Agrobacterium tumefaciens mediate gene transfer; microprojectile bombardment, PEG mediated transformation of protoplasts, electroporation and the like. Reference is made to (U.S. Pat. No. 6,803,499, U.S. Pat. No. 6,777,589; Fromm et al (1990) Biotechnol. 8:833-839; Potrykus et al (1985) Mol. Gen. Genet. 199:169-177.
12. Production of Proteins
The present disclosure further relates to methods of producing the variant glucoamylases comprising transforming a host cell with an expression vector comprising a polynucleotide encoding a variant glucoamylase according to the disclosure, optionally culturing the host cell under conditions suitable for production of the variant glucoamylase and optionally recovering the glucoamylase.
In the expression and production methods of the present disclosure the host cells are cultured under suitable conditions in shake flask cultivation, small scale or large scale fermentations (including continuous, batch and fed batch fermentations) in laboratory or industrial fermentors, with suitable medium containing physiological salts and nutrients (see, e.g., Pourquie, J. et al., BIOCHEMISTRY AND GENETICS OF CELLULOSE DEGRADATION, eds. Aubert, J. P. et al., Academic Press, pp. 71-86, 1988 and Ilmen, M. et al., (1997) Appl. Environ. Microbiol. 63:1298-1306). Common commercially prepared media (e.g., Yeast Malt Extract (YM) broth, Luria Bertani (LB) broth and Sabouraud Dextrose (SD) broth) find use in the present disclosure. Representative culture conditions for bacterial and filamentous fungal cells are known in the art and may be found in the scientific literature and/or from the source of the fungi such as the American Type Culture Collection and Fungal Genetics Stock Center. In cases where a glucoamylase coding sequence is under the control of an inducible promoter, the inducing agent (e.g., a sugar, metal salt or antimicrobial), is added to the medium at a concentration effective to induce glucoamylase expression.
In some embodiments, the present disclosure relates to methods of producing the variant glucoamylase comprising cultivating a plant or plant cell comprising a polynucleotide encoding a variant glucoamylase according to the disclosure under conditions suitable for the production of the variant and optionally recovering the glucoamylase.
In some embodiments, in order to evaluate the expression of a variant glucoamylase by a cell line that has been transformed with a polynucleotide encoding a variant glucoamylase encompassed by the disclosure, assays are carried out at the protein level, the RNA level and/or by use of functional bioassays particular to glucoamylase activity and/or production. Some of these assays include Northern blotting, dot blotting (DNA or RNA analysis), RT-PCR (reverse transcriptase polymerase chain reaction), or in situ hybridization, using an appropriately labeled probe (based on the nucleic acid coding sequence) and conventional Southern blotting and autoradiography.
In addition, the production and/or expression of a variant glucoamylase may be measured in a sample directly, for example, by assays directly measuring reducing sugars such as glucose in the culture medium and by assays for measuring glucoamylase activity, expression and/or production. In particular, glucoamylase activity may be assayed by the 3,5-dinitrosalicylic acid (DNS) method (see, Goto et al., (1994) Biosci. Biotechnol. Biochem. 58:49-54). In additional embodiments, protein expression, is evaluated by immunological methods, such as immunohistochemical staining of cells, tissue sections or immunoassay of tissue culture medium, (e.g., by Western blot or ELISA). Such immunoassays can be used to qualitatively and quantitatively evaluate expression of a glucoamylase. The details of such methods are known to those of skill in the art and many reagents for practicing such methods are commercially available.
The glucoamylases of the present disclosure may be recovered or purified from culture media by a variety of procedures known in the art including centrifugation, filtration, extraction, precipitation and the like.
13. Compositions
The variant glucoamylases may be used in enzyme compositions including but not limited to starch hydrolyzing and saccharifying compositions, cleaning and detergent compositions (e.g., laundry detergents, dish washing detergents, and hard surface cleaning compositions), alcohol fermentation compositions, and in animal feed compositions. Further the variant glucoamylases may be used in baking applications, such as bread and cake production, brewing, healthcare, textile, environmental waste conversion processes, biopulp processing, and biomass conversion applications.
In some embodiments, an enzyme composition including a variant glucoamylase encompassed by the disclosure obtained in culture media or recovered and purified from the culture medium will be optionally used in combination with any one or combination of the following enzymes—alpha amylases, proteases, pullulanases, isoamylases, cellulases, hemicellulases, xylanases, cyclodextrin glycotransferases, lipases, phytases, laccases, oxidases, esterases, cutinases, xylanases, granular starch hydrolyzing enzyme and other glucoamylases. In one embodiment, the proteases are acid fungal proteases (AFP). In a further embodiment, the acid fungal proteases are from Trichoderma (e.g., NSP-24, see also US 2006/0154353, published Jul. 13, 2006, incorporated herein by reference). In a further embodiment, the phytase is from Buttiauxiella. spp. (e.g., BP-17, see also variants disclosed in PCT patent publication WO 2006/043178).
In some representative compositions, the variant glucoamylases of the disclosure will be combined with an alpha amylase, such as fungal alpha amylases (e.g., Aspergillus sp.) or bacterial alpha amylases (e.g., Bacillus sp. such as B. stearothermophilus, B. amyloliquefaciens and B. licheniformis) and variants and hybrids thereof. In one embodiment the alpha amylase is an acid stable alpha amylase. In one embodiment, the alpha amylase is a granular starch hydrolyzing enzyme (GSHE). In one embodiment, the alpha amylase is Aspergillus kawachi alpha amylase (AKAA), see U.S. Pat. No. 7,037,704. Commercially available alpha amylases contemplated for use in the compositions of the disclosure are known and include GZYME G997, SPEZYME FRED, SPEZYME XTRA, STARGEN (Danisco US, Inc, Genencor Division), TERMAMYL 120-L and SUPRA (Novozymes, Biotech.) and VIRIDIUM (Diversa).
In other embodiments, the variant glucoamylases of the disclosure may be combined with other glucoamylases. In some embodiments, the glucoamylases of the disclosure will be combined with one or more glucoamylases derived from strains of Aspergillus or variants thereof, such as A. oryzae, A. niger, A. kawachi, and A. awamori; glucoamylases derived from strains of Humicola or variants thereof, particularly H. grisea, such as the glucoamylase having at least 90%, 93%, 95%, 96%, 97%, 98% and 99% sequence identity to SEQ ID NO: 3 disclosed in WO 05/052148; glucoamylases derived from strains of Talaromyces or variants thereof, particularly T. emersonii; glucoamylases derived from strains of Athelia and particularly A. rolfsii; glucoamylases derived from strains of Penicillium, particularly P. chrysogenum. 
14. Uses
In particular, the variant glucoamylases may be used for starch conversion processes, and particularly in the production of dextrose for fructose syrups, specialty sugars and in alcohol and other end-product (e.g., organic acid, ascorbic acid, and amino acids) production from fermentation of starch containing substrates (G. M. A van Beynum et al., Eds. (1985) STARCH CONVERSION TECHNOLOGY, Marcel Dekker Inc. NY). Dextrins produced using variant glucoamylase compositions of the disclosure may result in glucose yields of at least 80%, at least 85%, at least 90% and at least 95%. Production of alcohol from the fermentation of starch substrates using glucoamylases encompassed by the disclosure may include the production of fuel alcohol or portable alcohol. In some embodiments, the production of alcohol will be greater when the variant glucoamylase is used under the same conditions as the parent glucoamylase. In some embodiments, the production of alcohol will be between about 0.5% and 2.5% better, including but not limited to 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%. 1.7%, 1.8%, 1.9%, 2.0%, 2.1%, 2.2%, 2.3%, and 2.4% more alcohol than the parent glucoamylase.
In one representative embodiment, the variant glucoamylases of the disclosure will find use in the hydrolysis of starch from various plant-based substrates, which are used for alcohol production. In some embodiments, the plant-based substrates will include corn, wheat, barley, rye, milo, rice, sugar cane, potatoes and combinations thereof. In some embodiments, the plant-based substrate will be fractionated plant material, for example a cereal grain such as corn, which is fractionated into components such as fiber, germ, protein and starch (endosperm) (U.S. Pat. No. 6,254,914 and U.S. Pat. No. 6,899,910). Methods of alcohol fermentations are described in THE ALCOHOL TEXTBOOK, A REFERENCE FOR THE BEVERAGE, FUEL AND INDUSTRIAL ALCOHOL INDUSTRIES, 3rd Ed., Eds K. A. Jacques et al., 1999, Nottingham University Press, UK. In certain embodiments, the alcohol will be ethanol. In particular, alcohol fermentation production processes are characterized as wet milling or dry milling processes. In some embodiments, the variant glucoamylase will be used in a wet milling fermentation process and in other embodiments the variant glucoamylase will find use in a dry milling process.
Dry grain milling involves a number of basic steps, which generally include: grinding, cooking, liquefaction, saccharification, fermentation and separation of liquid and solids to produce alcohol and other co-products. Plant material and particularly whole cereal grains, such as corn, wheat or rye are ground. In some cases the grain may be first fractionated into component parts. The ground plant material may be milled to obtain a coarse or fine particle. The ground plant material is mixed with liquid (e.g., water and/or thin stillage) in a slurry tank. The slurry is subjected to high temperatures (e.g., 90° C. to 105° C. or higher) in a jet cooker along with liquefying enzymes (e.g., alpha amylases) to solublize and hydrolyze the starch in the grain to dextrins. The mixture is cooled down and further treated with saccharifying enzymes, such as glucoamylases encompassed by the instant disclosure, to produce glucose. The mash containing glucose may then be fermented for approximately 24 to 120 hours in the presence of fermentation microorganisms, such as ethanol producing microorganism and particularly yeast (Saccharomyces spp.). The solids in the mash are separated from the liquid phase and alcohol such as ethanol and useful co-products such as distillers' grains are obtained.
In some embodiments, the saccharification step and fermentation step are combined and the process is referred to as simultaneous saccharification and fermentation or simultaneous saccharification, yeast propagation and fermentation.
In other embodiments, the variant glucoamylase is used in a process for starch hydrolysis wherein the temperature of the process is carried out at a temperature of between 30° C. and 75° C. and also at a temperature of between 40° C. and 65° C. at a pH range of between pH 3.0 and pH 6.5. The fermentation processes in some embodiments include milling of a cereal grain or fractionated grain and combining the ground cereal grain with liquid to form a slurry that is then mixed in a single vessel with a variant glucoamylase according to the disclosure and optionally other enzymes such as but not limited to alpha amylases, other glucoamylases, phytases, proteases, pullulanases, isoamylases or other enzymes having granular starch hydrolyzing activity and yeast to produce ethanol and other co-products (U.S. Pat. No. 4,514,496, WO 04/081193 and WO 04/080923).
In some embodiments, the disclosure pertains to a method of saccharifying a liquid starch solution, which comprises an enzymatic saccharification step using a variant glucoamylase of the disclosure.
The present disclosure also provides an animal feed comprising at least one variant glucoamylase encompassed by the disclosure. Methods of using a glucoamylase enzyme in the production of feeds comprising starch are provided in WO 03/049550, filed Dec. 13, 2002 (herein incorporated by reference in its entirety). Briefly, the glucoamylase variant is admixed with a feed comprising starch. The glucoamylase is capable of degrading resistant starch for use by the animal.
Other objects and advantages of the present disclosure are apparent from the present Specification.
EXAMPLES
The following examples are provided in order to demonstrate and further illustrate certain representative embodiments and aspect of the present disclosure and are not to be construed as limiting the scope thereof.
In the disclosure and experimental section that follows, the following abbreviations apply: GA (glucoamylase); GAU (glucoamylase unit); wt % (weight percent); ° C. (degrees Centigrade); rpm (revolutions per minute); H2O (water); dH2O (deionized water); dIH2O (deionized water, Milli-Q filtration); aa or AA (amino acid); by (base pair); kb (kilobase pair); kD (kilodaltons); g or gm (grams); μg (micrograms); mg (milligrams); μL (microliters); ml and mL (milliliters); mm (millimeters); μm (micrometer); M (molar); mM (millimolar); μM (micromolar); U (units); V (volts); MW (molecular weight); sec(s) or s(s) (second/seconds); min(s) or m(s) (minute/minutes); hr(s) or h(s) (hour/hours); DO (dissolved oxygen); ABS (Absorbance); EtOH (ethanol); PSS (physiological salt solution; m/v (mass/volume); and MTP (microtiter plate); N (Normal); DP1 (monosaccharides); DP2 (disaccharides); DP>3 (oligosaccharides, sugars having a degree of polymerization greater than 3); ppm (parts per million).
The methods used to provide variants are described below. However, it should be noted that different methods may be used to provide variants of a parent molecule and the disclosure is not limited to the methods used in the examples. It is intended that any suitable means for making variants and selection of variants may be used.
pNPG Glucoamylase Activity Assay for 96-Well Microtiter Plates:
The reagent solutions were: NaAc buffer: 200 mM sodium acetate buffer pH 4.5; Substrate: 50 mM p-nitrophenyl-α-D-glucopyranoside (Sigma N-1377) in NaAc buffer (0.3 g/20 ml) and stop solution: 800 mM glycine-NaOH buffer pH 10. 30 μl filtered supernatant was placed in a fresh 96-well flat bottom MTP. To each well 50 μl NaAc buffer and 120 μl substrate was added and incubated for 30 minutes at 50° C. (Thermolab systems iEMS Incubator/shaker HT). The reaction was terminated by adding 100 μl stop solution. The absorbance was measured at 405 nm in a MTP-reader (Molecular Devices Spectramax 384 plus) and the activity was calculated using a molar extinction coefficient of 0.011 μM/cm.
Thermal Stability Assay:
Crude supernatant (100 μl) was added to 100 μl 50 mM NaAc buffer pH 4.5. the sample was equally divided over 2 MTP. One MTP (initial plate) was incubated for 1 hr at 4° C. and the other MTP (residual plate) was incubated at 60° C. (Thermolab systems iEMS Incubator/Shaker HT) for 1 hr. The residual plate was chilled for 15 min on ice. Activity is measured on both plates using the ethanol application assay described below, with the following modification: the amount of sample taken for the thermostability assay is 25 μl and the amount of 30 mM NaAc buffer pH 4.0 is 35 μl.
Thermostability is calculated as % residual activity as follows:




  
    
      
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The crude supernatant material is tested for remaining glucose in the culture medium after the growth period. If remaining glucose is found, the absorbance value is subtracted from the measured absorbance values of both the initial activity as the residual activity.
Bradford Assay for Protein Quantification in 96-Well Microtiter Plates:
The reagent solution was Bradford Quickstart work solution (BioRad cat#500-0205). 100 μl of 10 kD-filtered supernatant was placed in a fresh 96-well flat bottom plate. To each well 200 μl reagent was added and incubated for 5 minutes at room temperature. The absorbance was measured at 595 nm in a MTP-reader (Molecular Devices Spectramax 384 plus). Protein concentrations were calculated according to a Bovine Serum Albumin (BSA) (0-50 μg/ml) standard curve.
Hexokinase Activity Assay:
Hexokinase cocktail: 10-15 minutes prior to use, 90 ml water was added to a BoatIL container glucose HK R1(IL test glucose (HK) kit, Instrument Laboratory #182507-40) and gently mixed. 85 μl of Hexokinase cocktail were added to 100 μl of dH2O. 15 μl of sample were added to the mixtures and incubated for 5 mins in the dark at room temperature. Absorbance was read at 340 nm in a MTP-reader. Glucose concentrations were calculated according to a glucose (0-1 mg/ml) standard curve.
Assay Conditions Ethanol Application:
To prepare the 8% stock solution, 8 g of soluble corn starch (Sigma #S4180) was suspended in 40 ml dH2O at room temperature. Fifty milliliters of boiling dH2O was added to the slurry in a 250 ml flask and cooked for 5 minutes. The starch solution was cooled to 25° C. and the volume adjusted to 100 ml with dH2O. The 4% (m/v) soluble starch working solution was prepared by diluting (1:1) the stock solution with 100 mM sodium acetate buffer pH 3.7.
For the screening assay, 5 ml crude supernatant was diluted with 175 μl, 50 mM NaAc buffer pH 4.5 in a flat bottom 96-well MTP. Sixty microliters of this dilution was added to 120 μl 4% soluble corn starch and incubated at 900 rpm for 2 hrs at 32° C. (Thermolab systems iEMS Incubator/Shaker HT). The reaction was stopped by adding 90 μl 4° C.-cold Stop Solution (800 mM Glycine-NaOH buffer, pH 10). The sample was placed on ice. Starch was spun down at 1118×g at 15° C. for 5 minutes (SIGMA 6K15) and 15 μl supernatant was used in the Hexokinase activity assay described above to determine the glucose content.
The crude supernatant material was tested for remaining glucose in the culture medium after the growth period. If remaining glucose was found, the amount of glucose produced by the glucoamylase was not calculated.
Assay Conditions Sweetener Application:
To prepare the 8% stock solution, 8 g of soluble starch (Sigma #S4180) was suspended in 40 ml water at room temperature. Then, 50 ml of boiling dH2O was added to the slurry in a 250 ml flask and cooked for 5 mins. The starch solution was cooled to 25° C. and the volume adjusted to 100 ml with dH2O. The 4% (m/v) soluble starch working solution was prepared by diluting stock solution 1:1 with 100 mM sodium acetate buffer pH 4.5.
Fifty microliters of 80 mM NaAc buffer pH 4.5 were placed in a fresh 96-well flat bottom plate. To each well, 120 μl 4% soluble cornstarch and 5 μl 10 kD-filtered supernatant was added and incubated for 1 hr at 60° C. The reaction was stopped by adding 90 μl 4° C. cold Stop Solution (800 mM Glycine-NaOH buffer, pH 10.0). The sample was placed on ice for 30 mins Starch was spun down at 716 rpm at 15° C. for 5 minutes (Sigma 6K15, centrifuge) and 15 μl of the supernatant was used in the Hexokinase activity assay described herein to determine glucose content.
Example 1
Construction of the pREP3Y-TrGA Vector
The TrGA expression cassette composed of the DNA sequence (SEQ ID NO: 4) encoding the TrGA signal peptide, the pro-sequence, and the mature protein, including the catalytic domain, linker region and starch binding domain, was cloned into pDONRTM201, a Gateway® Entry vector (Invitrogen, Carlsbad, Calif., USA). The TrGA expression cassette was cloned into the Gateway compatible destination vector pREP3Y-DEST (FIG. 3) by the Gateway® LR recombination reaction.
The pRep3Y-TrGA expression vector (FIG. 3B) enabled the expression of the TrGA protein (SEQ ID NO: 2) in Schizosaccharomyces pombe. 
Sixty-five TrGA site saturated mutagenesis (SSM) libraries were constructed using the pDONR-TrGA entry vector (FIG. 2) as a template and the primers listed in Table 2. The mutagenesis primers used in the experiments all contain the triple DNA sequence code NNS (N=A,C,T,G and S═C or G) at the position that corresponds with the codon of the TrGA sequence to be mutated (SEQ ID NO: 2) and initiated random incorporation of nucleotides at that position. Construction of each SSM library started with two PCR amplifications using the Gateway forward (pDONR201-FW) and a specific reverse mutagenesis primer (Table 3), and Gateway reversed (pDONR201-RV) primer and a specific forward mutagenesis primer (Table 2) (equal positions for the mutagenesis primers). Phusion high fidelity DNA polymerase (Finnzymes O Y, Espoo, Finland) was used for PCR amplification (0.2 μM primers, 25 cycles) according to the protocol provided by Finnzymes. Briefly, 1 μl (SEQ ID NO: 1), DNA fragment of both specific PCR mixes, both targeted at the same codon were added to 48 μl of fresh PCR reaction solution together with primers Gateway FW and Gateway RV (Invitrogen) and mixed. This fusion PCR amplification (22 cycles) resulted in a linear expression cassette DNA fragment with a specific TrGA codon randomly mutated and unique Gateway recombination sites on both ends. Purification of this DNA fragment (ChargeSwitch® PCR clean-up, Invitrogen, Carlsbad USA) and a BP recombination reaction (Invitrogen, Carlsbad, USA) with pDONR201 (Invitrogen) generated a circular multimeric DNA (entry vector) that was subsequently transformed to E. coli Max efficiency DH5α (Invitrogen) and plated on 2×TY medium [Bacto Tryptone (Difco) 16 g/L, Bacto Yeast Extract (Difco) 10 g/L, NaCL 5 g/L] supplemented with 50 μg/mL kanamycin.







TABLE 2







Forward primers used to generate TrGA SSM libraries











SEQ


Prim-

ID


ers
DNA SEQUENCE 5′ TO 3′
NO:





pDONR-
TCGCGTTAACGCTAGCATGGATCTC
10


201-




FW




 


D4F
TCTGTTGACNNSTTCATCAGCACCGAGACGC
11


 


F5F
TCTGTTGACGACNNSATCAGCACCGAGACGCCTA
12


 


I12F
ATCAGCACCGAGACGCCTNNSGCACTGAACAATCTTCTTT
13


 


D24F
CTTTGCAATGTTGGTCCTNNSGGATGCCGTGCATTCGGCA
14


 


F29F
CCTGATGGATGCCGTGCANNSGGCACATCAGCTGGTGCGG
15


 


I43F
ATTGCATCTCCCAGCACANNSGACCCGGACTACTATTACA
16


 


D44F
GCATCTCCCAGCACAATTNNSCCGGACTACTATTACATGT
17


 


P45F
TCTCCCAGCACAATTGACNNSGACTACTATTACATGTGGA
18


 


D46F
CCCAGCACAATTGACCCGNNSTACTATTACATGTGGACGC
19


 


Y47F
AGCACAATTGACCCGGACNNSTATTACATGTGGACGCGAG
20


 


Y49F
ATTGACCCGGACTACTATNNSATGTGGACGCGAGATAGCG
21


 


W51F
CCGGACTACTATTACATGNNSACGCGAGATAGCGCTCTTG
22


 


Y70F
GACCGCTTCACCGAAACGNNSGATGCGGGCCTGCAGCGCC
23


 


Q75F
ACGTACGATGCGGGCCTGNNSCGCCGCATCGAGCAGTACA
24


 


R76F
TACGATGCGGGCCTGCAGNNSCGCATCGAGCAGTACATTA
25


 


P94F
CTCCAGGGCCTCTCTAACNNSTCGGGCTCCCTCGCGGACG
26


 


D100F
CCCTCGGGCTCCCTCGCGNNSGGCTCTGGTCTCGGCGAGC
27


 


K114F
AAGTTTGAGTTGACCCTGNNSCCTTTCACCGGCAACTGGG
28


 


F116F
GAGTTGACCCTGAAGCCTNNSACCGGCAACTGGGGTCGAC
29


 


N119F
CTGAAGCCTTTCACCGGCNNSTGGGGTCGACCGCAGCGGG
30


 


R122F
TTCACCGGCAACTGGGGTNNSCCGCAGCGGGATGGCCCAG
31


 


R125F
AACTGGGGTCGACCGCAGNNSGATGGCCCAGCTCTGCGAG
32


 


N146F
AAGTGGCTCATCAACAACNNSTATCAGTCGACTGTGTCCA
33


 


Q148F
CTCATCAACAACAACTATNNSTCGACTGTGTCCAACGTCA
34


 


Y169F
CTCAACTATGTTGCCCAGNNSTGGAACCAAACCGGCTTTG
35


 


Q172F
GTTGCCCAGTACTGGAACNNSACCGGCTTTGACCTCTGGG
36


 


F175F
TACTGGAACCAAACCGGCNNSGACCTCTGGGAAGAAGTCA
37


 


W178F
CAAACCGGCTTTGACCTCNNSGAAGAAGTCAATGGGAGCT
38


 


El80F
GGCTTTGACCTCTGGGAANNSGTCAATGGGAGCTCATTCT
39


 


V181F
TTTGACCTCTGGGAAGAANNSAATGGGAGCTCATTCTTTA
40


 


Q208F
CTTGCTGCCACTCTTGGCNNSTCGGGAAGCGCTTATTCAT
41


 


S211F
ACTCTTGGCCAGTCGGGANNSGCTTATTCATCTGTTGCTC
42


 


W228F
TGCTTTCTCCAACGATTCNNSGTGTCGTCTGGTGGATACG
43


 


N242F
GACTCCAACATCAACACCNNSGAGGGCAGGACTGGCAAGG
44


 


E243F
TCCAACATCAACACCAACNNSGGCAGGACTGGCAAGGATG
45


 


R245F
ATCAACACCAACGAGGGCNNSACTGGCAAGGATGTCAACT
46


 


I292F
GTCGACTCCTTCCGCTCCNNSTACGGCGTGAACAAGGGCA
47


 


G294F
TCCTTCCGCTCCATCTACNNSGTGAACAAGGGCATTCCTG
48


 


K297F
TCCATCTACGGCGTGAACNNSGGCATTCCTGCCGGTGCTG
49


 


R309F
GCTGCCGTCGCCATTGGCNNSTATGCAGAGGATGTGTACT
50


 


Y310F
GCCGTCGCCATTGGCCGGNNSGCAGAGGATGTGTACTACA
51


 


D313F
ATTGGCCGGTATGCAGAGNNSGTGTACTACAACGGCAACC
52


 


V314F
GGCCGGTATGCAGAGGATNNSTACTACAACGGCAACCCTT
53


 


Y315F
CGGTATGCAGAGGATGTGNNSTACAACGGCAACCCTTGGT
54


 


Y316F
TATGCAGAGGATGTGTACNNSAACGGCAACCCTTGGTATC
55


 


N317F
GCAGAGGATGTGTACTACNNSGGCAACCCTTGGTATCTTG
56


 


W321F
TACTACAACGGCAACCCTNNSTATCTTGCTACATTTGCTG
57


 


K340F
GATGCCATCTACGTCTGGNNSAAGACGGGCTCCATCACGG
58


 


K341F
GCCATCTACGTCTGGAAGNNSACGGGCTCCATCACGGTGA
59


 


T350F
TCCATCACGGTGACCGCCNNSTCCCTGGCCTTCTTCCAGG
60


 


Q356F
ACCTCCCTGGCCTTCTTCNNSGAGCTTGTTCCTGGCGTGA
61


 


T363F
GAGCTTGTTCCTGGCGTGNNSGCCGGGACCTACTCCAGCA
62


 


S368F
GTGACGGCCGGGACCTACNNSAGCAGCTCTTCGACCTTTA
63


 


S369F
ACGGCCGGGACCTACTCCNNSAGCTCTTCGACCTTTACCA
64


 


N376F
AGCTCTTCGACCTTTACCNNSATCATCAACGCCGTCTCGA
65


 


Y395F
CTCAGCGAGGCTGCCAAGNNSGTCCCCGCCGACGGTTCGC
66


 


A398F
GCTGCCAAGTACGTCCCCNNSGACGGTTCGCTGGCCGAGC
67


 


S401F
TACGTCCCCGCCGACGGTNNSCTGGCCGAGCAGTTTGACC
68


 


R408F
CTGGCCGAGCAGTTTGACNNSAACAGCGGCACTCCGCTGT
69


 


N409F
GCCGAGCAGTTTGACCGCNNSAGCGGCACTCCGCTGTCTG
70


 


T412F
TTTGACCGCAACAGCGGCNNSCCGCTGTCTGCGCTTCACC
71


 


H418F
ACTCCGCTGTCTGCGCTTNNSCTGACGTGGTCGTACGCCT
72


 


W421F
TCTGCGCTTCACCTGACGNNSTCGTACGCCTCGTTCTTGA
73


 


R433F
TTGACAGCCACGGCCCGTNNSGCTGGCATCGTGCCCCCCT
74


 


I436F
ACGGCCCGTCGGGCTGGCNNSGTGCCCCCCTCGTGGGCCA
75


 


S451F
AGCGCTAGCACGATCCCCNNSACGTGCTCCGGCGCGTCCG
76
















TABLE 3







Primers used to generate TrGA SSM libraries











SEQ




ID


Primer
DNA sequence 5′ to 3′
NO:





pDON-
GTAACATCAGAGATTTTGAGACAC
 77


201-




RV




 


D4R
GCGTCTCGGTGCTGATGAASNNGTCAACAGA
 78


 


F5R
TAGGCGTCTCGGTGCTGATSNNGTCGTCAACAGA
 79


 


I12R
AAAGAAGATTGTTCAGTGCSNNAGGCGTCTCGGTGCTGAT
 80


 


D24R
TGCCGAATGCACGGCATCCSNNAGGACCAACATTGCAAAG
 81


 


F29R
CCGCACCAGCTGATGTGCCSNNTGCACGGCATCCATCAGG
 82


 


I43R
TGTAATAGTAGTCCGGGTCSNNTGTGCTGGGAGATGCAAT
 83


 


D44R
ACATGTAATAGTAGTCCGGSNNAATTGTGCTGGGAGATGC
 84


 


P45R
TCCACATGTAATAGTAGTCSNNGTCAATTGTGCTGGGAGA
 85


 


D46R
GCGTCCACATGTAATAGTASNNCGGGTCAATTGTGCTGGG
 86


 


Y47R
CTCGCGTCCACATGTAATASNNGTCCGGGTCAATTGTGCT
 87


 


Y49R
CGCTATCTCGCGTCCACATSNNATAGTAGTCCGGGTCAAT
 88


 


W51R
CAAGAGCGCTATCTCGCGTSNNCATGTAATAGTAGTCCGG
 89


 


Y70R
GGCGCTGCAGGCCCGCATCSNNCGTTTCGGTGAAGCGGTC
 90


 


Q75R
TGTACTGCTCGATGCGGCGSNNCAGGCCCGCATCGTACGT
 91


 


R76R
TAATGTACTGCTCGATGCGSNNCTGCAGGCCCGCATCGTA
 92


 


P94R
CGTCCGCGAGGGAGCCCGASNNGTTAGAGAGGCCCTGGAG
 93


 


D100R
GCTCGCCGAGACCAGAGCCSNNCGCGAGGGAGCCCGAGGG
 94


 


K114R
CCCAGTTGCCGGTGAAAGGSNNCAGGGTCAACTCAAACTT
 95


 


F116R
GTCGACCCCAGTTGCCGGTSNNAGGCTTCAGGGTCAACTC
 96


 


N119R
CCCGCTGCGGTCGACCCCASNNGCCGGTGAAAGGCTTCAG
 97


 


R122R
CTGGGCCATCCCGCTGCGGSNNACCCCAGTTGCCGGTGAA
 98


 


R125R
CTCGCAGAGCTGGGCCATCSNNCTGCGGTCGACCCCAGTT
 99


 


N146R
TGGACACAGTCGACTGATASNNGTTGTTGATGAGCCACTT
100


 


Q148R
TGACGTTGGACACAGTCGASNNATAGTTGTTGTTGATGAG
101


 


Y169R
CAAAGCCGGTTTGGTTCCASNNCTGGGCAACATAGTTGAG
102


 


Q172R
CCCAGAGGTCAAAGCCGGTSNNGTTCCAGTACTGGGCAAC
103


 


F175R
TGACTTCTTCCCAGAGGTCSNNGCCGGTTTGGTTCCAGTA
104


 


W178R
AGCTCCCATTGACTTCTTCSNNGAGGTCAAAGCCGGTTTG
105


 


El80R
AGAATGAGCTCCCATTGACSNNTTCCCAGAGGTCAAAGCC
106


 


V181R
TAAAGAATGAGCTCCCATTSNNTTCTTCCCAGAGGTCAAA
107


 


Q208R
ATGAATAAGCGCTTCCCGASNNGCCAAGAGTGGCAGCAAG
108


 


S211R
GAGCAACAGATGAATAAGCSNNTCCCGACTGGCCAAGAGT
109


 


W228R
CGTATCCACCAGACGACACSNNGAATCGTTGGAGAAAGCA
110


 


N242R
CCTTGCCAGTCCTGCCCTCSNNGGTGTTGATGTTGGAGTC
111


 


E243R
CATCCTTGCCAGTCCTGCCSNNGTTGGTGTTGATGTTGGA
112


 


R245R
AGTTGACATCCTTGCCAGTSNNGCCCTCGTTGGTGTTGAT
113


 


I292R
TGCCCTTGTTCACGCCGTASNNGGAGCGGAAGGAGTCGAC
114


 


G294R
CAGGAATGCCCTTGTTCACSNNGTAGATGGAGCGGAAGGA
115


 


K297R
CAGCACCGGCAGGAATGCCSNNGTTCACGCCGTAGATGGA
116


 


R309R
AGTACACATCCTCTGCATASNNGCCAATGGCGACGGCAGC
117


 


Y310R
TGTAGTACACATCCTCTGCSNNCCGGCCAATGGCGACGGC
118


 


D313R
GGTTGCCGTTGTAGTACACSNNCTCTGCATACCGGCCAAT
119


 


V314R
AAGGGTTGCCGTTGTAGTASNNATCCTCTGCATACCGGCC
120


 


Y315R
ACCAAGGGTTGCCGTTGTASNNCACATCCTCTGCATACCG
121


 


Y316R
GATACCAAGGGTTGCCGTTSNNGTACACATCCTCTGCATA
122


 


N317R
CAAGATACCAAGGGTTGCCSNNGTAGTACACATCCTCTGC
123


 


W321R
CAGCAAATGTAGCAAGATASNNAGGGTTGCCGTTGTAGTA
124


 


K340R
CCGTGATGGAGCCCGTCTTSNNCCAGACGTAGATGGCATC
125


 


K341R
TCACCGTGATGGAGCCCGTSNNCTTCCAGACGTAGATGGC
126


 


T350R
CCTGGAAGAAGGCCAGGGASNNGGCGGTCACCGTGATGGA
127


 


Q356R
TCACGCCAGGAACAAGCTCSNNGAAGAAGGCCAGGGAGGT
128


 


T363R
TGCTGGAGTAGGTCCCGGCSNNCACGCCAGGAACAAGCTC
129


 


S368R
TAAAGGTCGAAGAGCTGCTSNNGTAGGTCCCGGCCGTCAC
130


 


S369R
TGGTAAAGGTCGAAGAGCTSNNGGAGTAGGTCCCGGCCGT
131


 


N376R
TCGAGACGGCGTTGATGATSNNGGTAAAGGTCGAAGAGCT
132


 


Y395R
GCGAACCGTCGGCGGGGACSNNCTTGGCAGCCTCGCTGAG
133


 


A398R
GCTCGGCCAGCGAACCGTCSNNGGGGACGTACTTGGCAGC
134


 


S401R
GGTCAAACTGCTCGGCCAGSNNACCGTCGGCGGGGACGTA
135


 


R408R
ACAGCGGAGTGCCGCTGTTSNNGTCAAACTGCTCGGCCAG
136


 


N409R
CAGACAGCGGAGTGCCGCTSNNGCGGTCAAACTGCTCGGC
137


 


T412R
GGTGAAGCGCAGACAGCGGSNNGCCGCTGTTGCGGTCAAA
138


 


H418R
AGGCGTACGACCACGTCAGSNNAAGCGCAGACAGCGGAGT
139


 


W421R
TCAAGAACGAGGCGTACGASNNCGTCAGGTGAAGCGCAGA
140


 


R433R
AGGGGGGCACGATGCCAGCSNNACGGGCCGTGGCTGTCAA
141


 


I436R
TGGCCCACGAGGGGGGCACSNNGCCAGCCCGACGGGCCGT
142


 


S451R
CGGACGCGCCGGAGCACGTSNNGGGGATCGTGCTAGCGCT
143









For each library, after overnight incubation at 37° C., colonies were pooled by resuspension of the clones in PSS. From the pooled E. coli transformants, total plasmid was isolated (Qiagen) using standard techniques. Briefly 1 μl of the plasmid solution was added to 1 μl of pRep3Y destination vector (FIG. 1A) solution and added to the LR CLONASETM II enzyme mix according to the protocol supplied by Invitrogen. A circular multimeric DNA was generated and transformed to E. coli Max EfficiencyDH5α as described by the supplier.
After overnight incubation at 37° C., 96 single colonies of each library were picked from 2×TY agar plates with 100 μg/ml ampicillin and grown for 24 hrs at 37° C. in a MTP containing 200 μL 2×TY medium with 100 μg/ml ampicillin. Cultures were used for sequence analyses (BaseClear B.V., Leiden, Netherlands).
The library numbers ranged from 1 to 65 with an addition referring to the codon of the TrGA sequence that is randomly mutated. After selection, each library included a maximum of 19 TrGA variants. These variants were individually transferred to Schizosaccharomyces pombe according to manufacturers instruction. (Zymo Research, Orange Calif. USA).
S. pombe transformations were plated on selective medium (EMM agar, Qbiogene, Irvine, USA Cat. No. 4110-232) and incubated at 28° C. for 4 days. Transformants were purified from the transformation plate by streaking the colonies on EMM agar.
Example 2
Description of the Growth Conditions and the Sample Pre-Treatment
S. pombe transformants were inoculated in 96 well microtiter plates (MTP) containing selective medium (2×EMM-C) [64.4 g/L EMM Broth (Qbiogene Cat. No: 4110-032), 0.62 g/L Complete Supplement Mixture (CSM-HIS-LEU-TRP, Qbiogene, Cat. No. 4530-122)] and incubated overnight at 28° C. From the overnight incubated microtiter plate, 200 μl of grown S. pombe culture was inoculated in 20 ml of 2×EMM-C liquid medium in a 100 ml shake flask and incubated for 4 days at 26° C. at 280 rpm in a Multitron shaking incubator (Infors AG, Bottmingen, Switzerland). From the grown culture, 2 ml of S. pombe culture was sampled and centrifuged for 5 min at 14,000 rpm (Sigma). The supernatant was transferred into a 10 kD Vivaspin 500 HT filter set-up (VivaScience AG, Hannover, Germany) and centrifuged for 25 min at 1000 g. The retentate was diluted back to the original start volume with 50 mM NaAc pH 4.5 supplemented with 0.015% Tween-80. This solution was used in the different assays.
Example 3
Construction of a Combinatorial Library of 4 Variants of TrGA
(A) Experiments were conducted for the construction of TrGA-variants carrying combinations of the following single site mutations: Q172F; Q208N; S211R and V314H. A review of the variants is shown below:
a) Q172F; Q208N
b) Q172F; S211R
c) Q172F; V314H
d) Q208N; S211R
e) Q208N V314H
f) S211R; V314H
g) Q172F; Q208N; S211R
h) Q172F; Q208N; V314H
i) Q172F; S211R; V314H
j) Q208N; S211R; V314H
k) Q172F; Q208N; S211R; V314H
The Quikchange® multi site-directed mutagenesis (QCMS) kit (Stratagene) was used to construct the library. The 5′ phosphorylated primers used to create the library are shown in Table 4. Optimal results in terms of incorporation of full length primers as well as significant reduction in primer-derived errors were obtained by the use of HPLC, PAGE or any other type of purified primers (Invitrogen).







TABLE 4







Primers used to construct selected combinatorial


variants











SEQ




ID


Primer
Sequence
NO:





I43R
GCATCTCCCAGCACACGAGACCCGGACTACTAT
144


 


I43Y
GCATCTCCCAGCACATACGACCCGGACTACTAT
145


 


R76L
GATGCGGGCCTGCAGCTGCGCATCGAGCAGTAC
146


 


N119T
CTGAAGCCTTTCACCGGCACCTGGGGTCGACCGCAGCGGG
147


 


N119Y
TGAAGCCTTTCACCGGCTACTGGGGTCGACCGCAGCGGG
148


 


N119D
CTGAAGCCTTTCACCGGCGACTGGGGTCGACCGCAGCGGG
149


 


N146
AGTGGCTCATCAACAACGASTATCAGTCGACTGTGT
150


 


N146T
AGTGGCTCATCAACAACACCTATCAGTCGACTGTGT
151


 


N146W
GTGGCTCATCAACAATGGTATCAGTCGACTGTGT
152


 


N146L
AGTGGCTCATCAACAACCTGTATCAGTCGACTGTGT
153


 


N146S
AGTGGCTCATCAACAACTCCTATCAGTCGACTGTGT
154


 


Q172D/E
TTGCCCAGTACTGGAACGASACCGGCTTTGACCTCTGG
155


 


Q172V/L
TTGCCCAGTACTGGAACSTGACCGGCTTTGACCTCTGG
156


 


Q172T
TTGCCCAGTACTGGAACACCACCGGCTTTGACCTCTGG
157


 


Q172R
TTGCCCAGTACTGGAACCGAACCGGCTTTGACCTCTGG
158


 


Q172C
TTGCCCAGTACTGGAACTGCACCGGCTTTGACCTCTGG
159









The template plasmid pDONR-TrGA (FIG. 2) was used to construct the combinatorial library using the QCMS kit of Stratagene. The library was constructed as described by the supplier with modified primer concentrations used in the reactions. Specifically, 4 μl pDONR-TrGA (25-50 ng) was mixed with 11 μl of sterile distilled water; 1.5 μl of dNTP; 2.5 μl of 10×QCMS-buffer; 1 μl of enzyme blend and 1 μl of each mutant primer mix giving a total of 100 ng of primers in each reaction. The PCR conditions were 95° C. for 1 min, followed by 30 cycles of 95° for 1 min, 55° C. for 1 min, and 65° C. for 6 min, in an MJ Research thermocycler using thin-walled 0.2 ml PCR tubes. The reaction product was digested with 1 μl of Dpn1 from the QCMS kit by incubating at 37° C. overnight. A PCR purification kit (Qiagen) was used for sample purification and a second round of digestion was carried out with Dpn1 (Stratagene) for 1 hour at 37° C.
The reaction mixture was transformed to into E. coli Max efficiency DH5α (Invitrogen) and plated on selective agar (2×TY supplemented with 50 μg kanamycin/ml). After overnight incubation at 37° C., 96 single colonies were picked for sequence analysis (BaseClear B. V., Leiden, Netherlands). The combinatorial variants were cloned and expressed in a T. reesei host strain as described below and in WO 06/060062.
(B) A further six combinatorial libraries (Table 5) were synthetically made by Geneart (Regensburg, Germany) and were tested for thermal stability and in ethanol and sweetener application assays as described herein.







TABLE 5





Combinatorial libraries
















1
D24E, L/I43F, R/D44H, N/F175H/V181K, L/V314D, H, K/T363R


2
D24L, W, Y/Q208F/I292F, L, N, V/G294A, I, Q/K297A/Y310F, Q, R


3
V181F, K, L/E243A, N, M, R, Y/I292F, L, N, V/K297A, D, H, M, N, 



Q/N317H/Y395Q, R


4
D24E, L, Y/V181F, K, L/Q208C/F/G294A, I, Q/T363R/N376Q/



N409K, W


5
D24E, L, Y/V181F, K, L/I292F, L, N, V/G294A, I, Q/E243A, M, N, R, 



Y/N409K, W


6
I43R/E243A, M, N, R, Y/I292F, L, N, V/G294A/K297A, D, H, M, N, 



Q, S, R, W, Y









Example 4
Variants with Improved Thermal Stability
The parent TrGA molecule under the conditions described had a residual activity between 15 and 44% (day-to-day variation). The performance index was calculated based on the TrGA thermostability of the same batch. The performance indices are the quotients PI=(Variant residual activity)/(TrGA residual activity). A performance index >1 indicates an improved stability. Variants that have a thermal stability performance index of more than 1.0 are shown in the following Table 6.







TABLE 6







Thermostability screening











PI Thermal




Stability, 



Variant
60° C., pH 4.5







D4P
1.05



I12E
1.09



I12Y
1.40



D24L
1.09



D24W
1.13



D24Y
1.03



I43R
1.28



D44N
1.06



D44Q
1.10



Q75N
1.09



R76K
1.03



N146D
1.20



N146E
1.24



N146L
1.10



N146V
1.28



N146W
1.17



Q148D
1.02



F175I
1.02



F175Y
1.06



E180A
1.41



E180D
1.02



E180G
1.13



E180I
1.41



E180L
1.38



E180M
1.10



E180N
1.27



E180Q
1.72



E180R
1.59



E180V
1.08



E180W
1.30



E180Y
1.31



V181I
1.20



V181K
1.12



V181L
1.06



V181Q
1.09



V181R
1.07



Q208F
1.06



Q208T
1.17



Q208V
1.15



S211D
1.10



S211E
1.02



S211I
1.31



S211M
1.90



E243A
1.19



E243H
1.04



E243M
1.53



E243N
1.35



E243P
1.06



E243R
1.21



E243S
1.09



E243T
1.48



E243Y
1.43



I292F
1.17



I292L
1.10



I292N
1.31



I292V
1.02



G294A
1.30



G294C
1.41



G294D
1.31



G294E
1.34



G294H
1.17



G294I
2.15



G294L
2.01



G294P
1.13



G294Q
1.91



G294R
1.34



G294V
1.10



K297A
1.47



K297C
1.10



K297D
1.50



K297F
1.24



K297G
1.25



K297H
1.63



K297L
1.62



K297M
1.62



K297N
1.87



K297Q
1.82



K297R
1.29



K297S
1.22



K297T
1.33



K297V
1.10



K297W
1.85



K297Y
1.71



R309S
1.08



Y310C
1.06



Y310F
1.35



Y310L
1.11



Y310Q
1.40



Y310R
1.61



Y315E
1.24



Y315H
1.48



Y315L
1.35



Y315N
1.17



Y315P
1.19



Y315Q
1.43



Y315T
1.34



Y316D
1.06



N317H
1.26



N317Q
1.09



K340H
1.02



K340R
1.09



K341I
1.10



K341V
1.07



T350G
1.08



T350P
1.08



T350S
1.33



Q356L
1.20



T363N
1.30



S368C
1.12



S368E
1.07



S368F
1.16



S368H
1.26



S368I
1.15



S368L
1.33



S368N
1.21



S368P
1.05



S368Q
1.10



S368R
1.14



S368T
1.15



S368W
1.16



S369A
1.22



S369D
1.05



S369F
1.20



S369G
1.05



S369K
1.12



S369L
1.49



S369M
1.36



S369N
1.25



S369P
1.16



S369R
1.12



S369T
1.25



N376F
1.12



N376G
1.26



N376H
1.21



N376K
1.40



N376L
1.34



N376P
1.05



N376Q
1.11



N376S
1.09



N376V
1.19



N376W
1.12



N376Y
1.05



Y395A
1.05



Y395C
1.02



Y395F
1.03



Y395G
1.13



Y395H
1.10



Y395L
1.50



Y395N
1.20



Y395Q
1.18



Y395R
1.14



Y395S
1.13



Y395T
1.04



A398C
1.10



A398D
1.39



A398F
1.05



A398G
1.17



A398H
1.33



A398I
1.41



A398K
1.47



A398L
1.44



A398N
1.23



A398P
1.38



A398Q
1.43



A398R
1.59



A398S
1.14



A398T
1.25



A398V
1.29



A398W
1.45



A398Y
1.38



S401A
1.12



S401E
1.08



S401I
1.05



S401N
1.12



S401P
1.15



S401R
1.25



S401T
1.26



S401V
1.18



R408A
1.14



R408E
1.41



R408G
1.15



R408H
1.12



R408I
1.19



R408K
1.80



R408L
1.55



R408N
1.09



R408Q
1.23



R408S
1.17



N409A
1.25



N409C
1.18



N409D
1.21



N409E
1.27



N409F
1.32



N409G
1.14



N409H
1.29



N409I
1.56



N409K
1.44



N409L
1.57



N409M
1.17



N409Q
1.03



N409R
1.29



N409V
1.11



N409W
1.58



T412L
1.10



S451R
1.01










Example 5
High Performing Variants from an Ethanol Screening Assay
Variants were tested in an ethanol screening assay using the assays described above. Table 7 shows the results of the screening assay for variants with a Performance Index (PI)>1.0 compared to the parent TrGA PI. The PI is a measure of specific activity (activity/mg enzyme). The PI of the specific activity is the quotient “Variant-specific activity/WT-specific activity.” The PI of the specific activity is 1.0 and a variant with a PI>1.0 has a specific activity that is greater than the parent TrGA. The specific activity is the activity measured by the ethanol screening assay divided by the results obtained in the Bradford assay described above.







TABLE 7







Ethanol Screening











P.I.



Variant
32° C., pH 4














D4A
1.07



D4C
1.08



D4E
1.23



D4L
1.34



D4R
1.18



D4S
1.17



F5C
1.35



I12L
1.19



I12R
1.13



D24E
1.60



D24L
1.19



D24W
1.03



D24Y
1.14



F29A
1.05



F29C
1.12



F29D
1.20



F29E
1.05



F29I
1.26



F29L
1.42



F29Q
1.01



F29S
1.07



F29V
1.06



I43D
1.14



I43F
1.33



I43R
1.21



I43Y
1.05



D44E
1.37



D44F
1.07



D44G
1.03



D44H
1.11



D44K
1.09



D44N
1.07



D44S
1.08



D44Y
1.07



Y70E
1.02



Y70G
1.06



Y70K
1.01



Y70M
1.36



Y70P
1.15



Y70R
1.40



Y70S
1.04



Q75A
1.10



Q75K
1.77



R76K
1.06



R76L
1.11



R76M
1.13



R76N
1.02



R76T
1.04



R76V
1.05



R76W
1.02



R76Y
1.05



D100A
1.08



D100I
1.14



D100L
1.03



D100M
1.12



D100N
1.06



D100P
1.09



D100Q
1.14



D100T
1.06



D100W
1.19



D100Y
1.05



N119E
1.02



N119F
1.03



N119Y
1.28



N146C
1.11



N146E
1.02



N146G
1.11



N146H
1.07



N146K
1.06



Q148H
1.10



Q148N
1.05



Q148V
1.18



Q148W
1.05



Q148Y
1.16



Y169D
1.18



Y169F
1.10



Y169H
1.05



Y169R
1.02



Q172E
1.08



Q172G
1.05



Q172R
1.22



Q172S
1.03



F175C
1.18



F175H
1.26



F175T
1.28



F175W
1.16



F175Y 
1.05



V181F 
1.28



V181K 
1.35



V181L 
1.37



V181R 
1.01



Q208A 
1.22



Q208C 
1.17



Q208F 
1.12



Q208H 
1.02



Q208I 
1.02



Q208L 
1.32



S211A 
1.30



S211E 
1.30



S211G 
1.05



S211L 
1.04



S211M 
1.05



S211R 
1.34



S211W 
1.07



S211Y 
1.08



E243A 
1.23



E243L 
1.20



E243M 
1.26



E243N 
1.28



E243R 
1.31



E243Y 
1.25



I292F 
1.23



I292H 
1.04



I292L 
1.21



I292N 
1.27



I292R 
1.02



I292V 
1.24



G294A 
1.91



G294I
1.92



G294Q
1.99



K297A
1.82



K297D
1.87



K297H
1.79



K297M
1.91



K297N
1.87



K297Q
1.85



K297R
1.71



K297S
1.72



K297W
1.70



K297Y
1.80



R309L
1.43



Y310F
1.05



Y310Q
1.16



Y310R
1.24



V314D
1.10



V314F
1.04



V314H
1.31



V314K
1.08



V314L
1.02



V314N
1.05



V314R
1.06



Y316R
1.42



Y316W
1.05



N317H
1.14



N317K
1.02



N317S
1.03



N317T
1.23



K340D
1.33



K340T
1.16



K341D
1.04



K341F
1.64



K341G
1.64



K341L
1.04



K341N
1.05



K341S
1.06



T350A
1.56



T350D
1.04



T350E
1.59



T350H
1.03



T350N
1.06



T350Q
1.05



T350R
1.02



Q356D
1.69



Q356E
1.07



Q356H
1.03



Q356K
1.03



T363A
1.04



T363C
1.54



T363G
1.02



T363H
1.09



T363N
1.02



T363R
1.61



T363V
1.05



T363W
1.08



S368D
1.11



S368F
1.08



S368H
1.04



S368L
1.07



S368M
1.03



S368N
1.02



S368W
1.24



S369F
1.68



S369M
1.04



S369T
1.05



N376G
1.05



N376H
1.10



N376Q
1.16



N376S
1.06



N376T
1.12



N376V
1.64



Y395A
1.02



Y395C
1.05



Y395G
1.02



Y395Q
1.63



Y395R
1.20



Y395S
1.09



A398D
1.05



A398P
1.03



S401A
1.04



S401D
1.01



S401G 
1.04



S401N 
1.02



S401V 
1.06



N409K 
1.30



N409L 
1.04



N409W 
1.31



T412A 
1.04



T412G 
1.06



T412K 
1.05



R433Q 
1.16



I436A 
1.32



I436H 
1.02



I436T 
1.03



S451A 
1.03



S451M 
1.28



S451T 
1.09



S451Y 
1.03










Example 6
High Performing Variants from a Sweetener Screening Assay
Variants were tested in a sweetener screening assay as described hereinabove. Table 8 shows the results of the screening assay wherein variants with a Performance Index (PI)>1.00 compared to the parent TrGA PI are shown. The PI is a measure of specific activity (activity/mg enzyme). The PI of the specific activity is the quotient “Variant-specific activity/WT-specific activity.” The PI of the specific activity is 1.0 and a variant with a PI>1.0 has a specific activity that is greater than the parent TrGA.







TABLE 8







Sweetener screening











P.I. 60° C., 



Variant
pH 4.5














D4E
1.09



D4L
1.03



D4S
1.07



D24E
1.45



D24L
1.31



D24Y
1.01



I43D
1.05



I43F
1.31



I43R
1.28



D44E
1.09



D44H
1.12



D44N
1.31



Y70F
1.26



Y70L
1.22



Q75K
1.12



R76K
1.11



R76M
1.03



R76P
1.13



R76T
1.11



R76W
1.07



D100Y
1.04



N119E
1.12



N119Y
1.01



N146D
1.05



N146E
1.11



Q148D
1.02



Q148W
1.05



Q172H
1.05



Q172Y
1.03



F175H
1.42



F175Y
1.11



V181A
1.10



V181F
1.01



V181K
1.43



V181L
1.42



Q208C
1.08



Q208F
1.20



Q208H
1.11



Q208L
1.03



Q208N
1.03



Q208S
1.06



Q208T
1.12



S211H
1.16



S211M
1.16



S211R
1.34



S211W
1.09



E243A
1.06



E243F
1.01



E243N
1.05



E243R
1.14



E243S
1.09



E243Y
1.07



R245A
1.01



I292N
1.04



I292V
1.12



G294A
1.06



G294Q
1.02



K297A
1.04



K297D
1.10



K297Q
1.07



V314D
1.22



V314H
1.85



V314K
1.34



V314L
1.13



V314N
1.08



V314R
1.20



V314Y
1.05



Y316R
1.20



N317H
1.25



N317K
1.03



K340D
1.21



K340E
1.05



K341D
1.08



K341G
1.22



K341L
1.08



K341N
1.08



K341S
1.12



T350H
1.03



T350L
1.04



Q356D
1.31



Q356E
1.04



Q356K
1.05



T363C
1.08



T363G
1.04



T363N
1.02



T363R
1.50



S368G
1.04



S368M
1.03



N376G
1.02



N376Q
1.07



Y395Q
1.01



A398H
1.03



A398S
1.03



S401A
1.01



N409K
1.19



N409T
1.02



N409W
1.01



T412G
1.06



T412S
1.03



I436D
1.02



I436Q
1.06



I436T
1.16



S451D
1.01



S451E
1.09



S451F
1.02



S451H
1.11



S451T
1.11










Example 7
Construction of Vectors and Transformation into Trichoderma reesei Host Cells
A. Construction of Expression Vectors Comprising a Polynucleotide Encoding a Variant GA.
The TrGA expression cassette comprising the DNA sequence SEQ ID NO: 4 was cloned into pDONRTM 201, a Gateway® Entry vector (Invitrogen, Carlsbad, Calif.). The TrGA expression cassette was cloned into the Gateway compatible destination vector pTrex3g-DEST (FIG. 5), which is also described in WO 06/060062, by the Gateway® LR recombination reaction. The pTrex3g-TrGA expression vector (FIG. 5) enabled the expression of the TrGA protein (SEQ ID NO: 2) in a Trichoderma reesei host. Vectors were constructed, which included modified TrGA cDNA coding at least the following variants (1) V314H; (2) S211R; (3) Q208N and (4) Q172F.
B. Transformation.
An expression vector containing a variant GA was transformed into a T. reesei host strain derived from RL-P37 (IA52) and having various gene deletions (Δ cbh1, Δcbh2, Δegl1, Δegl2) using particle bombardment by the PDS-1000/Helium System (BioRad Cat. No. 165-02257). The protocol is outlined below, and reference is also made to examples 6 and 11 of WO 05/001036.
A suspension of spores (approximately 5×108 spores/ml) from the strain of T. reesei was prepared. One hundred to two hundred microliters of spore suspension were spread onto the center of plates of Minimal Medium (MM) acetamide medium. The MM acetamide medium had the following composition: 0.6 g/L acetamide; 1.68 g/L CsCl; 20 g/L glucose; 20 g/L KH2PO4; 0.6 g/L CaCl2.2H2O; 1 ml/L 1000× trace elements solution: 20 g/L agar; and pH 5.5; 1 ml/L 400× trace element salt solution; citric acid 175 g/L, FeSO4.7H2O 200 g/L, ZnSO4.7H2O 16 g/L, CuSO4.5H2O 3.2 g/L, MnSO4H2O 1.4 g/L, H3BO3 0.8 g/L. The spore suspension was allowed to dry on the surface of the MM acetamide medium.
Transformation followed the manufacturers instruction. Briefly, 60 mg of M10 tungsten particles were placed in a microcentrifuge tube. 1 mL of ethanol was added and allowed to stand for 15 minutes. The particles were centrifuged at 15,000 rpm for 15 seconds. The ethanol was removed and the particles were washed three times with sterile dH2O before 1 mL of 50% (v/v) sterile glycerol was added. 25 μl of tungsten particle suspension was placed into a microcentrifuge tube. While continuously vortexing, the following were added: 0.5-5 μl (100-200 ng/μl) of plasmid DNA, 25 μl of 2.5 M CaCl2 and 10 μl of 0.1 M spermidine. The particles were centrifuged for 3 seconds. The supernatant was removed and the particles were washed with 200 μl of 70% (v/v) ethanol and centrifuged for 3 seconds. The supernatant was removed and 24 μl 100% ethanol was added, mixed by pipetting, and the tube was placed in an ultrasonic bath, 8 μl aliquots of particles were removed and placed onto the center of macrocarrier disks that were held in a desiccator. Once the tungsten/DNA suspension had dried the microcarrier disk was placed in the bombardment chamber along with the plate of MM acetamide with spores and the bombardment process was performed according to the manufacturers instructions. After bombardment of the plated spores with the tungsten/DNA particles, the plates were incubated at 28° C. Transformed colonies were picked to fresh plates of MM acetamide after 4 days (Pentilla et al. (1987) Gene 61: 155-164).
C. Demonstration of GA Activity from the Expressed Variant TrGA in Transformed Cells.
After 5 days growth on MM acetamide plates transformants displaying stable morphology were inoculated into 250 ml shake flasks containing 30 ml of Proflo medium. Proflo medium contained: 30 g/L α-lactose; 6.5 g/L (NH4)2SO4; 2 g/L KH2PO4; 0.3 g/L MgSO4.7H2O; 0.2 g/L CaCl2.2H2O; 1 ml/L 400× trace element salt solution: citric acid 175 g/L, FeSO4.7H2O 200 g/L, ZnSO4.7H2O 16 g/L, CuSO4.5H2O 3.2 g/L, MnSO4.H2O 1.4 g/L, H3BO3 0.8 g/L; 2 ml/L 10% Tween 80; 22.5 g/L ProFlo cottonseed flour (Traders protein, Memphis, Tenn.); 0.72 g/L CaCO3. After two days growth at 28° C. and 140 rpm, 10% of the Proflo culture was transferred to a 250 ml shake flask containing 30 ml of Lactose Defined Media. The composition of the Lactose defined Media was as follows 5 g/L (NH4)2SO4; 33 g/L1,4-piperazinebis(propanesulfonic acid) buffer; 9 g/L casamino acids; 4.5 g/L KH2PO4; 1.0 g/L MgSO4.7H2O; 5 ml/L Mazu DF60-P antifoam (Mazur Chemicals, Ill.); 1000× trace element solution; pH 5.5; 40 ml/L of 40% (w/v) lactose solution was added to the medium after sterilization. The Lactose Defined medium shake flasks were incubated at 28° C., 140 rpm for 4-5 days.
Samples of the culture supernatant were mixed with an appropriate volume of 2× sample loading buffer with reducing agent. Mycelium was removed by centrifugation and the supernatant was analyzed for total protein (BCA Protein Assay Kit, Pierce Cat. No. 23225).
GA activity was measured using the p-nitrophenyl-alpha-D-glucopyranoside (pNPG) assay with pNPG as a substrate (Sigma N-1377). In this assay the ability of glucoamylase to catalyze the hydrolysis of p-nitrophenyl-alpha-D-glucopyranoside (pNPG) to glucose and p-nitrophenol is measured. At an alkaline pH, the nitrophenol forms a yellow color that is proportional to glucoamylase activity and is monitored at 405 nm and compared against an enzyme standard measured as a GAU (Elder, M. T. and Montgomery R. S., Glucoamylase activity in industrial enzyme preparations using colorimetric enzymatic method, Journal of AOAC International, vol. 78(2), 1995). One GAU is defined as the amount of enzyme that will produce 1 gm of reducing sugar calculated as glucose per hour from a soluble starch substrate (4% ds) at pH 4.2 and 60° C.
The protein profile was determined by PAGE electrophoresis on NuPAGE® Novex 10% Bis-Tris Gel with MES SDS Running Buffer (Invitrogen, Carlsbad, Calif., USA).
Example 8
Small Scale Applications Testing of Selected Variants on Soluble Starch
Trichoderma reesei host strains expressing the single variants a) V314H, b) S211R, c) Q172F and d) Q208N were grown in fed-batch 14L fermentors at 34° C., pH 3.5 in nutrient media including glucose (Cerelose DE99), KH2PO4, MgSO4.7H2O, (NH4)2SO4, CaCl2.2H2O, trace elements and Mazu anti-foam (DF6000K). Upon glucose depletion growth temperature and pH were shifted to 28° C. and 4.0, respectively. Cell material was removed by filtration and culture supernatants were collected and concentrated to contain greater than 90% glucoamylase as total protein.
Various kinetic properties were determined for glucose production on soluble potato starch at pH 4.3 at 32° C. and at 60° C. and compared to the wild-type TrGA. Each of the four variants demonstrated increased Vmax (μM glucose/sec) values as compared to the wild type (TrGA) indicating elevated catalytic rates (kcat (sec−1)). FIG. 6 illustrates the Vmax of two replicates for each temperature tested.
Example 9
Method to Determine the Performance on EtOH Production of Variants
Validation of the screening was performed on the variants that were identified as having a higher performance index as compared to the parent TrGA (see Table 7/8) using a novel small scale Ethanol application test. Twenty-four variants derived from site evaluation and combinatorial (Table 9) libraries were selected and transformed directly into T. reesei for expression and testing on larger scale. The variants were tested for thermal unfolding using Differential Scanning calorimetry (DSC analysis described herein below) and performance using a novel secondary small-scale ethanol application assay. The method consisted of two steps: 1) injection of variants onto an anion exchange column to accurately determine the protein concentration; and 2) titration of variants with three different TrGA concentrations (0.3-0.15-0.075 g/28 g ds) in order to calculate their performance on ethanol production relative to the wild type molecule.







TABLE 9







List of combinatorial variants








variant
mutation





LR8
Q172F/Q208N


LR6
Q172F/Q208N/V314H


LR12
Q172F/S211R


SW3-1
D24E/I43R/D44N/F175H/V181L/V314H/T353R


SW3-2
D24L/I43F/D44N/F175H/V181L/V314H/T353R


ET4-1
D24L/Q208Q/I292V/G294A/K297A/Y310R


ET4-2
D24W/Q208F/I292V/G294Q/K297A/Y310R


ET5-1
V181L/E243A/I292N/K297N/N317N/Y395Q


ET5-2
V181L/E243R/I292F/K297A/N317N/Y395Q


ET7-1
D24Y/V181L/Q208C/G294A/T353R/N375N/N409W


ET7-2
D24L/V181L/Q208C/G294A/T353R/N375Q/N409W


ET8-1
D24E/V181K/E243Y/I292V/G294Q/N409K


ET8-2
D24E/V181F/E243R/I292N/G294I/N409W


ET9-1
I043R/E243R/I292F/G294A/K297A


ET9-2
I043R/E243R/I292L/G294A/K297M









Protein Purification and Determination

A crude enzyme preparation was purified using an AKTA explorer 100 FPLC system (Amersham Biosciences, Piscataway, N.J.). β-Cyclodextrin was (Sigma-Aldrich, Zwijndrecht, The Netherlands; 85.608-8) coupled to epoxy-activated Sepharose beads (GE Healthcare, Diegem, Belgium; 17-0480-01). The column was used to capture glucoamylases from the enzyme preparation. Enzyme was eluted from the beads using 25 mM Tris buffer pH 7.5 or 50 mM sodium acetate buffer pH 4.3 containing 10 mM α-cyclodextrin (Sigma, 28705). Purified samples were analyzed by SDS-PAGE. To accurately determine the protein concentration of the variants an FPLC based protein determination method was developed. The protein concentration of the purified marker TrGA molecule was first determined using a standard Bradford protocol (Bio-Rad cat#500-0205). Subsequently, purified samples were injected onto a ResourceQ_1 ml column (GE Healthcare) and enzyme was eluted with 25 mM Tris pH buffer containing 500 mM NaCl. Peak area was determined and the protein concentration was calculated relative to the peak area of the TrGA standard with known concentration.
Small Scale EtOH Application
Table 10 summarizes the production of ethanol and sugars (DP1, DP2, DP>3) by different combinatorial variants. A sample of corn mash liquefact obtained and diluted to 26% DS using thin stillage. The pH of the slurry was adjusted to pH 4.3 using 4N sulphuric acid. A 100 g aliquot of mash was placed into a 32° C. water bath and allowed to equilibrate. After 100 μl 400 ppm urea addition, 1 ml purified variant TrGA enzyme sample (150 μg/ml) or purified TrGA (300, 150, 75 μg/ml) was added to each corn mash sample. Finally, 333 μl of 30 minutes hydrated 15 g in 45 ml DI water solution of Red Star Red yeast (Lesaffre yeast Corp. Milwaukee, Wis.) was added to each sample. Samples were taken at 5, 21, 28, 48 and 52 hours and analyzed by HPLC using an Aminex HPX-87H column 9 (Bio-Rad).
Ethanol and Carbohydrate Determinations
A 2 ml eppendorf centrifuge tube was filled with fermentor beer and cooled on ice for 10 minutes. The sample was centrifuged for 3 minutes at 14.000×g and 500 μl of the supernatant was transferred to a test tube containing 50 μl of kill solution (1.1 N sulfuric acid) and allowed to stand for 5 minutes. 5.0 ml of water was added to the test tube and then filtered into a 0.22 μm filter plate (multiscreen, Millipore, Amsterdam, the Netherlands) and run on HPLC. Column Temperature: 60° C.; mobile phase: 0.01 N sulfuric acid; flow rate 0.6 ml/min; detector: RI; injection volume: 20 μl. The column separates molecules based on charge and molecular weight; DP1 (monosaccharides); DP2 (disaccharides); DP3 (trisaccharides); DP>3 (oligosaccharides sugars having a degree of polymerization greater than 3); succinic acid; lactic acid; glycerol; methanol; ethanol.
DSC Analysis
The melting temperature of purified enzyme samples (0.2-0.4 mg/ml) was determined using Differential Scanning calorimetry (DSC).







TABLE 10







Production of ethanol and saccharides














DP > 3
DP2
DP1
ethanol



hours
(m/v)%
(m/v)%
(m/v)%
(v/v)%















TrGA (0.3 mg)
5.5
3.46
2.70
0.91
1.02



21.5
3.40
0.50
0.06
6.80



28.5
1.68
1.46
0.07
8.13



46
0.04
0.71
0.06
10.21



52.5
0.04
0.45
0.03
10.96


TrGA (0.150 mg)
5.5
3.40
2.43
0.15
1.00



21.5
3.78
0.21
0.03
4.23



28.5
3.86
0.20
0.03
5.07



46
2.73
0.52
0.06
7.86



52.5
1.70
0.87
0.04
7.92


TrGA (0.075 mg)
5.5
3.43
2.16
−0.01
0.94



21.5
3.54
0.20
0.03
3.10



28.5
3.43
0.18
0.03
3.14



46
3.93
0.18
0.05
4.65



52.5
4.01
0.18
0.03
4.79


ET7-1
5.5
3.45
2.53
0.21
1.00



21.5
3.94
0.22
0.04
4.77



28.5
3.89
0.23
0.04
5.58



46
1.58
1.22
0.06
8.64



52.5
0.62
1.50
0.04
9.14


LR8
5.5
3.43
2.50
0.17
1.00



21.5
3.96
0.22
0.04
4.79



28.5
3.86
0.21
0.04
6.21



46
1.27
1.11
0.07
9.17



52.5
0.45
1.24
0.04
8.73


LR12
5.5
3.47
2.51
0.16
1.05



21.5
3.86
0.22
0.04
4.44



28.5
3.94
0.22
0.04
5.30



46
2.09
1.08
0.07
8.56



52.5
0.99
1.52
0.04
9.16


LR6
5.5
3.37
2.44
0.18
0.96



21.5
3.88
0.21
0.04
4.44



28.5
3.90
0.20
0.04
5.10



46
2.44
0.64
0.08
8.59



52.5
1.27
1.01
0.04
8.97


ET8-1
5.5
3.46
2.53
0.22
0.99



21.5
3.99
0.21
0.04
4.86



28.5
3.90
0.21
0.04
5.76



46
1.29
1.11
0.08
8.94



52.5
0.47
1.25
0.04
9.56


ET7-2
5.5
3.57
2.46
0.17
1.02



21.5
4.26
0.21
0.03
4.21



28.5
4.37
0.20
0.04
5.14



46
3.87
0.27
0.05
7.21



52.5
3.27
0.33
0.03
8.07









Table 11 represents the final ethanol yields and the performance of the variants at 0.15 mg dosage. The performance was calculated by interpolation of the 0.3 mg and 0.15 mg values of the TrGA by the values of the variants.







TABLE 11







Ethanol Yields












EtOH
performance 



variant
%(v/v)
relative to TrGA















TrGA 0.3 mg
10.21




TrGA 0.15 mg
7.86
1.00



TrGA 0.075 mg 
4.65




ET7-1
8.64
1.33



LR8
9.17
1.56



LR12
8.56
1.30



LR6
8.59
1.31



ET8-1
8.94
1.46



ET7-2
7.21
0.72










All combinatorial variants except ET7-2 performed better than TrGA wild type. LR8 performed the best with a 1.56 improved performance.
Table 12 gives an overview of all single site and combinatorial variants tested using the small-scale ethanol application assay. Variants that are shaded in Table 12 had a better performance than TrGA and also had a higher thermal unfolding temperature (dTm).







TABLE 12







Performance and thermal unfolding of variants relative to TrGA















The results showed that Chromatography (FPLC) was a useful tool to accurately determine the protein concentration. The results also showed that titration of variants with three TrGA concentrations was a valuable method to determine the performance of variants on small scale. Seven variants performed better than TrGA wild type (see Table 12) and also had a higher thermal unfolding temperature and the variants that did not perform as well as TrGA also had a lower Tm.
Example 10
Specific Activity Determination of a Selected Set of Combinatorial and Single Site Variants and Substrate Specificity of LR8
The specific activity of a set of the combinatorial variants and several single site variants that were used to construct combinatorial variants was analyzed (Table 13). LR8 (PI 1.56 determined with small scale application assay) was further studied with respect to substrate specificity. This was done by setting up an MTP assay to determine the glucose production rates of GA variants and to determine substrate specificity of the LR8 variant. The MTP assay was found to discriminate between variants and all variants except ET7-1 showed higher rates than the wild-type (wt) Trichoderma reesei glucoamylase. Further, several variants (LR8/ET8/Q172F) performed 20-30% better than TrGA. LR8 performed better on soluble corn starch and two different samples of corn mash liquefact compared to wild-type.
Substrates used in the following experiments were soluble corn starch stock solution prepared as follows: 8 g soluble corn starch (Sigma # S4180) was dissolved in 100 ml milliQ water and heated in a microwave for 1 minute. The dispersion was boiled for 5 minutes and after cooling the volume was adjusted to 100 ml. 4% soluble corn starch was prepared by diluting the stock solution 1:1 with 100 mM NaAc buffer pH 4. In one experiment, a corn liquefact substrate (NE) was prepared using a moisture analyzer to measure % ds, then substrate was diluted 7.5× with 50 mM NaAc to finally obtain 4% ds. The substrate was centrifuged for 5′ at 2000×g and the supernatant was filtered with a 0.22 μm filter. In another experiment, a corn liquefact substrate (BSE) was prepared in the same way, except that the substrate was diluted 10× before centrifugation.
The enzyme was diluted using the Stock solution of 150 μg enzyme/ml (3 μg/180 μl reaction mixture). Solutions were further diluted with 50 mM NaAc pH 4.0 as follows: 300 ng (10×), 200 ng, 150 ng, 100 ng, 75 ng, 50 ng, 25 ng, 10 ng/180 μl reaction mixture
The assay was performed as follows: 40 μl 50 mM NaAc pH 4.0, 120 μl 4% soluble corn starch, and 20 μl enzyme were added to each well. Samples were incubated for 2 hr at 32° C. 900 rpm and terminated on ice after addition of 90 μl 800 mM glycine-NaOH buffer pH 10 for 5 mM The plate was centrifuged for 5 mM at 2000 rpm at 15° C. To a fresh plate, 85 μl milliQ water and 100 μl hexokinase cocktail (Il test glucose (HK) kit, Instrumental Laboratory #182507-40) and 20 μl supernatant were added. For a glucose (0-1 mg/ml) calibration line 20 μl glucose stock was added instead. Plates were incubated for 10 min at room temperature in the dark followed by absorption measurement at 340 nm using the Spectramax.







TABLE 13







performance relative to wt















graph1
300
200
150
100
75
50
25
10 ng GA


















TrGA
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00


ET7-1
0.94
0.98
0.91
0.92
0.63
0.38
3.50
0.53


LR8
0.93
1.00
1.22
0.91
0.64
0.06
−0.89
−3.99


LR12
0.94
0.99
1.17
0.83
0.66
−0.15
−0.99
0.62


LR6
0.94
1.01
1.16
0.99
0.55
0.17
−1.67
−4.36


ET8-1
0.87
0.95
1.29
1.02
0.62
−0.05
−1.64
−3.42


ET7-2
0.94
1.00
1.24
0.93
0.50
0.22
−0.62
−1.40


graph2


V314H
0.95
0.97
1.24
0.91
0.78
0.54
0.74
1.89


G294Q
0.98
0.99
1.03
1.21
1.10
0.19
−0.34
−1.12


S211R
0.97
0.99
1.21
1.05
0.94
0.27
−1.32
0.53


Q208N
0.97
1.01
1.13
0.95
0.90
−0.05
−1.01
−2.81


Q172F
0.99
1.04
1.31
1.23
1.32
0.38
−1.59
−2.20


G294I
0.91
0.96
1.07
1.25
0.80
0.09
−1.20
−3.66


P94N
0.98
1.00
1.24
1.09
1.05
0.29
6.29
−3.80


TrGA
1.00
1.00
1.00
1.00
1.00
1.00
1.00
1.00









The results of the assay to determine the glucose production rates of by GA variants are shown in FIGS. 7 and 8. In these figures, the relative performance to TrGA was calculated per amount of enzyme added. Conclusions were drawn from the linear region of the graph at 150 ng of enzyme. The results in FIGS. 7, 8 and Table 13 showed that LR8, ET8, ET7-2, S211R, Q172F and P94N performed better than wild-type over the linear range.
To determine the substrate specificity of LR8, the performance of LR8 and TrGA wild-type was tested on substrates (soluble corn starch, and the two corn mash substrates produced in Example 10) used in screening and application. When analyzed by HPLC, the substrates showed a difference in degree of polymerization (DP) pattern (see FIGS. 9-11). In NE and BSE DP1→=DP4 is present while soluble corn starch consist of at least four or more glucose molecules. On all substrates LR8 performed better than wild-type (see FIGS. 9, 10 and 11).
Example 11
Screening and Characterization of Variants Expressed in Trichoderma Reesei Using TrGA Site Evaluation Libraries (SELs) in the pTTT Vector
Additional TrGA variants, particularly variants with substitutions within the SBD, were created and screened directly in Trichoderma reesei. Similar to Example 1, another ten TrGA site saturated mutagenesis (SSM) libraries were constructed using the pDONR-TrGA entry vector as a template and primers listed in Table 14. The sites include: N61, G73, L417, T430, A431, E503, Q511, A535, A539, and N563. Among these sites, E503, Q511, A535, A539, and N563 are located within the starch binding domain of the TrGA. Subsequently, the recombination was performed with the pTTT-Dest vector (FIG. 14) using the LR CLONASETM II enzyme mix according to the protocol supplied by Invitrogen. The recombination products were transformed into E. coli Max efficiency DH5α (Invitrogen) and plated on 2×TY medium [Bacto Tryptone (Difco) 16 g/L, Bacto Yeast Extract (Difco) 10 g/L, NaCL 5 g/L] supplemented with 100 μg/mL ampicillin. After overnight incubation at 37° C., 96 single colonies of each library were picked from 2×TY agar plates with 100 μg/ml ampicillin and grown for 24 hrs at 37° C. in a MTP containing 200 μL 2×TY medium with 100 μg/ml ampicillin. Cultures were used for sequence analyses (ABI3100 sequence analyzer, Applied Biosystems). Each library contained from 15 to 19 different TrGA variants in the final expression vector. These variants were individually transformed into T. reesei.







TABLE 14







Primers used to generate additional TrGA SSM


libraries









AA-




posi-
F/



tion
R
DNA sequence 5′ to 3′





 61
F
AGATAGCGCTCTTGTCTTCAAGNNSCTCATCGACCGC


 


 61
R
CTTGAAGACAAGAGCGCTATC


 


 73
F
CTTCACCGAAACGTACGATGCGNNSCTGCAGCGCCGC


 


 73
R
CGCATCGTACGTTTCGGTGAA


 


417
F
CAGCGGCACTCCGCTGTCTGCGNNSCACCTGACGTGGT


 


417
R
CGCAGACAGCGGAGTGCCGCT


 


430
F
GTACGCCTCGTTCTTGACAGCCNNSGCCCGTCGGGCT


 


430
R
GGCTGTCAAGAACGAGGCGTA


 


431
F
CGCCTCGTTCTTGACAGCCACGNNSCGTCGGGCTGGC


 


431
R
CGTGGCTGTCAAGAACGAGGC


 


503
F
CTCCGTGGCCGTCACCTTCCACNNSCTCGTGTCGACACA


 


503
R
GTGGAAGGTGACGGCCACGGA


 


511
F
GCTCGTGTCGACACAGTTTGGCNNSACGGTCAAGGTG


 


511
R
GCCAAACTGTGTCGACACGAG


 


535
F
GAGCGCCGCCGTGGCTCTGGACNNSGTCAACTATGCCGATA


 


535
R
GTCCAGAGCCACGGCGGCGCTCGTGCT


 


539
F
GGCTCTGGACGCCGTCAACTATNNSGATAACCACCCCCTGT


 


539
R
ATAGTTGACGGCGTCCAGAGCCACGGC


 


563
F
CGTCGTGGAGTACAAGTACATCNNSGTGGGCCAAGATGGCTCC


 


563
R
GATGTACTTGTACTCCACGACGTCTCC









The SELs were transformed into T. reesei using the PEG-protoplast method (see, e.g., Pentillä et al. (1987) Gene 61: 155-164). The T. reesei host is a strain derived from RL-P37 (IA52) and having four gene deletions (Δ cbh1, Δcbh2, Δegl1, Δegl2; i.e., “quad-deleted;” see U.S. Pat. No. 5,847,276, WO 92/06184, and WO 05/001036). Transformation mixtures containing up to 600 ng of DNA and 1-5×105 protoplasts in a total volume of 25 μl were treated with 200 ml of 25% PEG solution, diluted with 2 volumes of 1.2 M sorbitol solution, mixed with 3% selective top agarose MM with acetamide and poured onto 2% selective agarose with acetamide either in 24 well microtiter plates. The plates were incubated at 28° C. for 5 to 8 days. Spores from the total population of transformants regenerated on each individual well were harvested from the plates using a solution of 0.85% NaCl, 0.015% Tween 80. Spore suspensions were used to inoculate fermentations in 96 well MTPs. In the case of 24 well MTPs, an additional plating step on a fresh 24 well MTP with selective acetamide MM was introduced in order to enrich the spore number.
The transformants were fermented in MTPs and the culture supernatants containing the expressed protein variants were used for assays. In brief, MTPs containing 200 μl of LD-GSM medium were inoculated in quadruplicate with spore suspensions of T. reesei transformants expressing TrGA variants (more than 104 spores per well). The plates were incubated at 28° C. with 230 rpm shaking and 80% humidity for 6 days. Culture supernatants were harvested by vacuum filtration. The supernatants were used in different assay for screening of variants with improved properties.
Variants exhibiting a performance index of more than 1.0 for thermal stability, specific activity, and both thermal stability and specific activity are shown in Tables 15-17.







TABLE 15







Thermal stability screening of additional TrGA variants











PI of thermal



Variants
stability














N061V
1.06



G073F
1.44



G073M
1.01



G073N
1.10



G073W
1.36



L417I
1.04



L417K
1.20



L417M
1.05



L417Q
1.04



L417R
1.20



L417V
1.07



L417Y
1.01



T430A
1.05



T430E
1.02



T430F
1.06



T430H
1.10



T430I
1.04



T430K
1.08



T430M
1.17



T430N
1.13



T430Q
1.05



T430R
1.13



T430S
1.17



T430V
1.05



A431I
1.03



A431N
1.03



A431P
1.08



A431R
1.08



A431V
1.03



E503A
1.43



E503C
1.39



E503S
1.02



E503T
1.04



E503V
1.68



Q511A
1.11



Q511D
1.12



Q511H
1.33



Q511N
1.14



Q511S
1.15



A535D
1.12



A535K
1.24



A535N
1.37



A535P
1.59



A535R
1.26



A539E
1.32



A539H
1.17



A539M
1.05



A539R
1.36



A539S
1.30



N563A
1.14



N563C
1.49



N563E
1.44



N563I
1.65



N563K
1.77



N563L
1.60



N563Q
1.29



N563T
1.31



N563V
1.53

















TABLE 16







Specific activity screening of additional TrGA variants.











PI of specific



Variants
activity














N061D
1.05



N0611
1.21



N061L
1.18



N061Q
1.08



N061V
1.11



N061W
1.02



G073C
1.02



G073L
1.07



G073W
1.03



L417A
1.12



L417D
1.19



L417E
1.10



L417F
1.08



L417G
1.19



L4171
1.10



L417K
1.02



L417Q
1.04



L417R
1.30



L417S
1.05



L417T
1.10



L417V
1.21



L417W
1.05



L417Y
1.10



T430A
1.19



T430E
1.15



T430F
1.09



T430G
1.16



T430H
1.15



T4301
1.06



T430K
1.24



T430M
1.16



T430N
1.07



T430Q
1.15



T430R
1.04



T430V
1.09



A431C
1.04



A431E
1.08



A431H
1.11



A4311
1.20



A431L
1.21



A431M
1.12



A431Q
1.22



A431R
1.11



A431S
1.09



A431W
1.04



A431Y
1.13



E503C
1.05



E503D
1.06



E503H
1.01



E503S
1.10



E503W
1.04



Q511C
1.07



Q511G
1.06



Q511H
1.05



Q5111
1.10



Q511K
1.09



Q511T
1.04



Q511V
1.04



A535E
1.19



A535F
1.06



A535G
1.02



A535K
1.07



A535L
1.02



A535N
1.04



A535P
1.14



A535R
1.22



A535S
1.06



A535T
1.04



A535V
1.04



A535W
1.09



A535Y
1.13



A539E
1.08



A539M
1.03



A539S
1.02



A539W
1.06



A539R
1.22

















TABLE 17







Additional TrGA variants displaying both increased thermal 


stability and specific activity












PI of
PI of




specific
thermal



Variants
activity
stability















N061V
1.11
1.06



G073W
1.03
1.36



L4171
1.10
1.04



L417K
1.02
1.20



L417Q
1.04
1.04



L417R
1.30
1.20



L417V
1.21
1.07



L417Y
1.10
1.01



T430A
1.19
1.05



T430E
1.15
1.02



T430F
1.09
1.06



T430H
1.15
1.10



T430I
1.06
1.04



T430K
1.24
1.08



T430M
1.16
1.17



T430N
1.07
1.13



T430Q
1.15
1.05



T430R
1.04
1.13



T430V
1.09
1.05



A431I
1.20
1.03



A431R
1.11
1.08



E503C
1.05
1.39



E503S
1.10
1.02



Q511H
1.05
1.33



A535K
1.07
1.24



A535N
1.04
1.37



A535P
1.14
1.59



A535R
1.22
1.26



A539E
1.08
1.32



A539M
1.03
1.05



A539R
1.22
1.36



A539S
1.02
1.30










Example 12
Characterization of a Selected Set of Single Site and Combinatorial Variants
Based on the results of Examples 4-6 and 11, a selected set of combinatorial variants, and single site variants were further characterized for their alter properties. The selected set includes single site and combinatorial variants with substitution(s) at: I43, D44, N61, G73, G294, L417, T430, A431, E503, Q511, A535, A539, and/or N563. Variants were purified from large-scale fermentation, and PIs of thermal stability and specific activities were determined Specifically, specific activities were determined using different substrates, including DP7, cornstarch, and liquefact. The results are shown in Table 18 and 19.







TABLE 18







PIs of a selected set of single site variants, each of which is from a 


500 ml fermentation













P.I.
P.I.
P.I.



P.I. DP7-
CornStarch-
Thermal
Liquefact-


Variants
FPLC
FPLC
Stability
FPLC














N61I
1.16
1.35
1.00
1.66


A431L
1.15
1.38
1.18
1.51


L417V
1.18
1.32
1.02
1.40


A431Q
1.06
1.20
0.92
1.24


G294C
1.01
0.84
0.94
1.23


N563K
1.07
1.12
1.97
1.15


Q511H
1.05
1.09
1.52
1.13


T430M
1.05
1.15
0.89
1.09


E503A
1.08
1.16
1.40
1.09


I43Q
1.11
1.24
0.94
1.08


A539R
1.15
1.37
1.43
1.08


I43R
1.03
1.07
1.41
1.07


L417R
1.23
1.27
1.51
1.04


T430A
1.13
1.35
1.23
1.04


G73F
1.06
1.06
1.45
1.03


D44R
0.97
1.06
1.46
0.98


N563I
1.09
1.22
2.06
0.92


D44C
0.80
0.82
0.96
0.91


E503V
1.17
1.07
1.66
0.88


A535R
1.09
1.44
1.47
0.85
















TABLE 19







PIs of a selected set of combinatorial variants

















P.I.







P.I.
Corn
P.I.
P.I.





DP7-
Starch-
Thermal
Liquefact-


Ferm.
Variant
Substitutions
FPLC
FPLC
Stability
FPLC
















100 ml
C4
G73F/T430A/E503V/Q511H
1.05
1.06
2.57
1.01


100 ml
C9
D44C/G73F/L417R/N563K
0.95
1.00
2.57
1.22


100 ml
C11
D44C/G73F/N563K
0.83
1.04
2.42


500 ml
ALL5
I43R/L417V/E503A/Q511H/A539R
1.22
1.48
2.37
1.87


100 ml
C15
D44C/L417R/N563K
0.95
1.09
2.30
0.99


100 ml
C8
D44C/G73F/N563K
0.87
1.05
2.28


500 ml
C5
D44C/G73F/N563K
0.90
0.71
2.16
0.97


500 ml
TS1
I43R/T430A/E503V/A535R/N563K


2.13


500 ml
C7
D44C/G73F/E503V/Q511H
0.90
0.79
2.10
0.89


500 ml
ALL1
I43Q/D44C/L417V/E503A/Q511H/A539R
1.00
1.27
1.95
1.59


500 ml
CS4
L417V/T430A/Q511H/A539R/N563I
1.21
1.53
1.95
1.73


100 ml
C2
I43R/G73F/T430A
1.09
1.25
1.91
1.18


500 ml
RB7
I43Q/T430A/Q511H/L417V/A539R
1.11
1.36
1.85
2.17


500 ml
C1
G73F/T430A/Q511H
1.20
1.07
1.84
0.96


500 ml
ALL2
I43Q/L417V/E503A/Q511H/A539R
1.16
1.52
1.84
1.96


500 ml
ALL6
I43R/N61I/L417V/E503A/Q511H/A539R
1.06
1.53
1.84
2.24


500 ml
ALL8
I43R/N61I/L417R/E503A/Q511H/A539R
1.10
1.53
1.84
2.11


500 ml
RB20
I43Q/Q511H/A539R/T430M/N61I/L417V
1.01
1.55
1.79
1.95


500 ml
ALL4
I43Q/N61I/L417V/E503A/Q511H/A539R
1.08
1.52
1.79
1.73


500 ml
CS3
L417V/T430A/Q511H/A535R/N563I
0.98
1.40
1.69
1.78


500 ml
RB9
I43Q/T430A/Q511H/A431L/E503A
0.93
1.39
1.69
1.55


500 ml
RB17
I43Q/Q511H/A539R/E503A
1.12
1.38
1.68
1.40


500 ml
ALL7
I43R/L417R/E503A/A539R
1.04
1.54
1.67
1.62


500 ml
TS4
I43R/T430A/E503A/Q511H/N563K
0.98
1.33
1.66
1.33


500 ml
RB18
I43Q/Q511H/A539R/T430M
1.06
1.44
1.64
1.40


100 ml
C12
I43R/T430A/
1.15
1.35
1.60
1.26


500 ml
RB16
I43Q/Q511H/A539R/N61I
1.11
1.43
1.60
1.34


500 ml
TS5
D44R/T430A/Q511H/A535R
0.89
1.22
1.59
1.21


500 ml
RB8
I43Q/T430A/Q511H/A431L/A539R
1.02
1.38
1.59
2.18


500 ml
RB11
I43Q/T430A/Q511H/L417V/A539R/
1.05
1.36
1.58
2.03




A431L


500 ml
CS1
L417V/T430A/A431L/Q511H/A535R/
1.08
1.71
1.56
2.35




A539R/N563I


500 ml
LQ5
L417R/A431L/Q511H
0.95
1.21
1.56
1.88


500 ml
CS2
L417V/T430A/A431Q/Q511H/A535R/
1.07
1.71
1.56
2.32




A539R/N563I


500 ml
TS2
D44R/E503A/Q511H/N563I
0.90
1.14
1.53
1.23


500 ml
RB15
I43Q/Q511H/A539R
1.19
1.42
1.52
1.69


500 ml
RB13
I43Q/Q511H/L417V
1.06
1.38
1.50
1.50


500 ml
RB5
I43Q/T430A/Q511H/A539R
0.99
1.45
1.47
1.59


500 ml
RB4
I43Q/T430A/Q511H/E503A
1.07
1.29
1.47
1.50


500 ml
RB19
I43Q/Q511H/A539R/T430M/N61I
1.00
1.50
1.47
1.88


500 ml
ALL3
I43Q/D44C/N61I/L417V/E503A/
0.82
1.30
1.43
1.64




Q511H/A539R


500 ml
LQ4
G294C/L417R/A431Q/Q511H
1.02
1.19
1.43
1.65


500 ml
RB2
I43Q/T430A/Q511H/L417V
1.09
1.38
1.42
1.87


500 ml
TS3
E503A/N563I/
0.94
1.17
1.39
1.04


500 ml
RB12
I43Q/Q511H/N61I
0.96
1.25
1.37
1.56


500 ml
RB14
I43Q/Q511H/A431L
1.01
1.31
1.37
1.55


500 ml
LQ6
L417V/A431Q/Q511H
0.94
1.33
1.35
2.05


100 ml
C14
I43Q/T430A/Q511H
1.09
1.34
1.33
1.46


500 ml
LQ3
G294C/L417V/A431L/Q511H
0.80
1.21
1.29
2.07


500 ml
RB3
I43Q/T430A/Q511H/A431L
1.20
1.25
1.29
1.70


500 ml
RB10
I43Q/T430A/Q511H/N61I/A539R/A431L
0.87
1.47
1.29
1.73


500 ml
RB1
I43Q/T430A/Q511H/N61I
1.19
1.15
1.27
1.47


100 ml
C13
I43Q/T430A/
1.07
1.29
1.10
1.45


500 ml
LQ1
G294C/L417R/A431L
0.90
1.08
1.04
1.77


500 ml
LQ2
G294C/L417V/A431Q
0.80
1.08
1.02
2.08


500 ml
RB6
I43Q/T430A/Q511H/N61I/A539R


0.94









Example 11
Crystal Structure of TrGA
The complete three dimensional structure of Trichoderma reesei (Hypocrea jecorina) glucoamylase (TrGA) was determined at 1.9 Å resolution. Table 20 shows the coordinates for the Trichoderma glucoamylase crystal structure. TrGA was crystallized in an intact form containing 599 residues and all post-translational modifications that would normally occur in the natural host. The crystal structure was produced and analyzed as follows:
For protein expression and purification, the gene encoding H. jecorina GA was cloned and expressed according to the protocols described in the U.S. Pat. No. 7,413,887.
The TrGA protein material used for all crystallization experiments was initially purified in one step by anion exchange chromatography as follows: concentrated culture supernatants of expressed TrGA, consisting of 180 mg/ml total protein, were prepared by diluting sample 1:10 in a 25 mM Tris-HCl, pH 8.0 buffer. A HiPrep 16/10 Q Sepharose FF column (GE Helthcare) was employed for the anion exchange purification. The HiPrep column was equilibrated with 4 column volumes (CV) starting buffer (25 mM Tris-HCl, pH 8.0) followed by application of 10 ml of the diluted protein sample. An 8 CV linear gradient of 0 to 140 mM NaCl in the running buffer (25 mM Tris-HCl, pH 8.0) was applied to elute bound protein. Bound TrGA eluted from the HiPrep Q sepharose column at a salt concentration of approximately 80 mM NaCl. Fractions containing pure TrGA protein were pooled and concentrated to 50 mg/ml using a 25 ml Vivaspin centrifugal concentration tube (Viva Science) with a molecular weight cutoff (MWCO) of 10 kD. Purified and concentrated TrGA material was buffer exchanged using a DG-10 desalting column (Bio-Rad) equilibrated with 50 mM sodium acetate buffer, pH 4.3. Protein concentrations were determined by measuring the absorbance at 280 nm. The initially purified and concentrated TrGA protein stock was thereafter stored at −20° C.
Two additional purification steps, on additional anion exchange purification, and a size exclusion purification, were introduced to enhance the crystability of the TrGA protein material. These two additional purification steps were performed as follows. In the first anion exchange purification step a 10 ml MonoQ column (GE Helthcare) was employed. A Sample of 1 ml of the initially purified and frozen TrGA material (50 mg protein) was thawed and the buffer was changed to 20 mM Tris-HCl, pH 8.0, by repeated dilution of the sample to 6 ml in the new buffer, followed by a concentration of the sample again to 0.5 ml using a 6 ml 5 kD MWCO concentration tube. The TrGA sample was diluted after the last concentration step in distilled water until a conductivity of the protein sample was reached that corresponded to the conductivity of the starting buffer of the anion purification, i.e. 25 mM Tris-HCl, pH 8.0. The MonoQ column was first equilibrated with 4 column volumes (CV) starting buffer, followed by application of the diluted protein sample to the column Bound protein was eluted from the MonoQ column by two different gradients. In the first a 4 CV linear pH gradient was applied where the pH of the starting buffer was decreased from 8.0 to 6.0. In the second gradient an 8 CV long salt gradient was applied in which the salt concentration was increased from 0 to 350 mM NaCl in the running buffer (25 mM Tris-HCl, pH 6.0). Bound TrGA was found to elute from the column during the second salt gradient at an approximate NaCl concentration of 150 mM. Fractions containing TrGA were pooled and concentrated to 2 ml using a 6 ml 5 kD MWCO Vivaspin concentration tube. The concentrated TrGA sample was thereafter applied to a Superdex 200 16/60 size exclusion column (GE Helthcare) equilibrated with 4 CV of 20 mM Tris-Cl, pH 8.0, and 50 mM NaCl, which also was used as running buffer. Fractions from the main elution peak after the size exclusion purification were pooled and concentrated to an approximate protein concentration of 7.5 mg/ml using a 6 ml 5 kD MWCO Vivaspin concentration tube.
For protein crystallization, the protein sample that was used to find the initial TrGA crystallization conditions was a sample of the TrGA material that was purified once by anion exchange purification and thereafter stored at −20° C. The TrGA protein sample was thawed and diluted with 50 mM sodium acetate buffer, pH 4.3, to approximately 12 mg/ml, prior to the initial crystallization experiments. The orthorhombic X-ray dataset, was used to solve the TrGA structure by molecular replacement (MR), and the high-resolution orthorhombic dataset, used for the final orthorhombic space group TrGA structure model. The orthorhombic TrGA crystals were found to grow in solution consisting of 25% PEG 3350, 0.20M ammonium acetate, 0.10M Bis-Tris pH 5.5 (reservoir solution), using the vapor-diffusion method with hanging drops (McPherson 1982), at 20° C. Crystallization drops were prepared by mixing equal amounts of protein solution (12 mg/ml) and reservoir solution to a final volume of 10 μl. The TrGA crystals were found to belong to the orthorhombic space group P212121 with approximate cell dimensions: a=52.2 Å, b=99.2 Å, c=121.2 Å, and have a calculated Vm of 2.3 (Matthews 1968) with one molecules in the asymmetric unit.
For X-ray data collection, the two orthorhombic TrGA datasets were collected from single crystals mounted in sealed capillary tubes, at room temperature. The initial lo-resolution orthorhombic TrGA X-ray dataset, used to solve the structure by molecular replacement methods (MR), was collected on a home X-ray source, an MSC/Rigaku (Molecular Structures Corp., The Woodlands, Tex.) Raxis IV++ image plate detector with focusing mirrors using Cu Kα radiation from a Rigaku RU200 rotating anode generator. This dataset was processed, scaled, and averaged using the d*trek software provided by MSC/Rigaku. The C centered monoclinic dataset was collected from a single frozen TrGA crystal at 100K, equilibrated in a cryo-protective agent comprised of 25% PEG 3350, 15% Glycerol 50 mM CaCl2 and 0.1 M Bis-Tris pH 5.5 as cryoprotectant, mounted in rayon-fiber loops, and plunge frozen in liquid nitrogen prior to transportation to the synchrotron. The high-resolution orthorhombic (1.9 Å) data set and the C centric monoclinic dataset (1.8 Å) were both collected at a synchrotron source, beam line 911:5 at MAX LAB in Lund, Sweden. Both datasets that were collected at a synchrotron source were processed with MOSFLM, and scaled with program SCALA included in the CCP4 program package (Collaborative Computational Project Number 4 1994). All subsequent data processing was performed using the CCP4 program package (Collaborative Computational Project Number 4 1994), unless otherwise stated. A set of 5% of the reflections from each data set was set aside and used for monitoring the R-free (Brünger, A (1992) Nature, 355:472-475).
The TrGA structure was initially solved by MR with the automatic replacement program MOLREP (Collaborative Computational Project Number 4 1994), included in the CCP4 program package, using the initial lo-resolution orthorhombic dataset, and using the coordinates of Aspergillus. awamori GA (AaGA) variant X100 (pdb entry 1GLM (Aleshin et al. (1994) J. Mol. Boil. 238: 575-591) as search model. The A. awamori GA search model was edited to remove all glycosylation moieties attached to the protein molecule as N- and O-glycosylations, and all solvent molecules before carrying out the MR experiments. All reflections between 36.8 and 2.8 Å resolution, from the initial lo resolution TrGA dataset, was used for the MR solution. The MR program found a single rotation function solution, with a maxima of 11.1σ above background, the next highest maxima was 3.8σ above the background. The translation function solution gave an R-factor of 48.7% and had a contrast factor of 17.4. The MR solution was refined for 10 cycles of restrained least squares refinement using the program Refmac 5.0 (Murshudov et al (1997) Acta Crystallogr. D53: 240-255). This lowered the crystallographic R-factor to 31.1% while the R-free value dropped from 42.2% to 41.1%.
The refined MR solution model was used to calculate an initial density map from the lo-resolution orthorhombic TrGA dataset. Electron density for a disulfide bridge between residues 19 and 26 of TrGA, a disulfide bridge not present in the A. awamori variant X100 structure model, could readily be identified in this electron density map. This was taken as an indication that the electron density map was of sufficient quality to be used to build a structure model of TrGA from its amino acid sequence. The initial TrGA structure model, based on the lo-resolution dataset, was refined with alternating cycles of model building using Coot (Emsley and Cowtan, (2004) Acta Crystallogr. D boil Crystallogr. 60: 2126-2132), and maximum likelihood refinement using Refmac 5.0.
The resolution of the initial TrGA structure model was extended to the resolution of the high-resolution orthorhombic dataset (1.9 Å) by refining the initial TrGA structure model against the high-resolution dataset for 10 cycles of restrained refinement using the program Refmac 5.0. Most water molecules in the structure models were located automatically by using the water picking protocols in the refinement programs, and then manually selected or discarded by inspection by eye. All structural comparisons were made with either Coot (Emsley and Cowtan (2004) supra) or O (Jones et al. (1991) Acta Crystallogr. A47: 110-119), and figures were prepared with PyMOL (Delano W. L. (2002) The PyMOL Molecular Graphics System. Palo Alto, Calif., USA; Delano Scientific).
From these results, it can be seen that the TrGA catalytic core segment followed the same (α/α)6-barrel topology described by Aleshin et al. 1992 for the AaGA, consisting of a double barrel of alpha helices with the C-terminal of the outer helix leading into the N-terminus of an inner helix. It was possible to identify key differences in the electron density such as the disulfide bridge between residues 19 and 26 and an insertion (residues 257-260) relative to AaGA. The segment comprising 80-100 also underwent extensive model rebuilding. One major glycosylation site was identified at Asn 171, which had up to four glycoside moieties attached. A similar glycosylation site was identified in AaGA. Additionally, the catalytic core containing three cis-peptides between residues 22-23, 44-45 and 122-123 were conserved between TrGA and AaGA. Overall there was an rms variation of 0.535 Å between 409 out of 453 Ca atoms when comparing the coordinates of the catalytic cores of TrGA and AaGA.
Example 14
Homology Between TrGA and AaGA
The crystal structure of the TrGA identified in Example 13, was superposed on the previously identified crystal structure of the Aspergillus awamori GA (AaGA). The AaGA crystal structure was obtained from the protein database (PDB) and the form of AaGA that was crystallized was the form containing only a catalytic domain. The structure of the Trichoderma reesei glucoamylase with all three regions intact was determined to 1.8 Angstrom resolution herein (see Table 15 and Example 12). Using the coordinates (see Table 20) the structure was aligned with the coordinates of the catalytic domain from Aspergillus awamorii strain X100 that was determined previously (Aleshin, A. E., Hoffman, C., Firsov, L. M., and Honzatko, R. B. 1994 Refined crystal structures of glucoamylase from Aspergillus awamori var. X100. J Mol Biol 238: 575-591 and the PDB). As seen in FIGS. 12 and 13 the structure of the catalytic domain overlapped very closely and allowed the identification of equivalent residues based on this structural superposition.
Based on this analysis, sites were identified that could be mutated in TrGA and result in increased stability and/or specific activity. These sites include 108, 124, 175 and 316 at the active site. Also identified were specific pairwise variants Y47W/Y315F and Y47F/Y315W. Other sites identified were I43, D44, P45, D46, R122, R125, V181, E242, Y310, D313, V314, N317, R408, and N409. Because of the high structural homology it is expected that beneficial variants found at sites in TrGA would have similar consequences in Aspergillus awamori and other homologous glucoamylases.
Various modifications and variations of the described methods and system of the disclosure will be apparent to those skilled in the art without departing from the scope and spirit of the disclosure. Although the disclosure has been described in connection with specific representative embodiments, it should be understood that the subject matters as claimed should not be unduly limited to such specific embodiments. Indeed, various modifications of the described modes for carrying out the disclosure that are obvious to those skilled in the art are intended to be within the scope of the following claims.







TABLE 20







CRYST1  52.185  99.232 121.240  90.00 90.00 90.00


ORIGX1    1.000000 0.000000 0.000000     0.00000


ORIGX2    0.000000 1.000000 0.000000     0.00000


ORIGX3    0.000000 0.000000 1.000000     0.00000


SCALE1    0.019163 −0.000001 −0.000001     0.00000


SCALE2    0.000000 0.010077 0.000000     0.00000


SCALE3    0.000000 0.000000 0.008248     0.00000

















ATOM
1
N
SER
A
1
−30.485
30.567
−21.185
1.00
37.11


ATOM
2
CA
SER
A
1
−30.568
29.350
−20.326
1.00
37.00


ATOM
3
CB
SER
A
1
−31.953
28.707
−20.424
1.00
37.27


ATOM
4
OG
SER
A
1
−32.137
28.089
−21.695
1.00
40.11


ATOM
5
C
SER
A
1
−29.519
28.345
−20.772
1.00
35.91


ATOM
6
O
SER
A
1
−29.043
28.415
−21.911
1.00
35.46


ATOM
7
N
VAL
A
2
−29.170
27.425
−19.867
1.00
34.51


ATOM
8
CA
VAL
A
2
−28.302
26.293
−20.179
1.00
33.56


ATOM
9
CB
VAL
A
2
−28.142
25.339
−18.955
1.00
33.84


ATOM
10
CG1
VAL
A
2
−27.349
24.103
−19.316
1.00
34.20


ATOM
11
CG2
VAL
A
2
−27.468
26.057
−17.827
1.00
34.79


ATOM
12
C
VAL
A
2
−28.846
25.506
−21.363
1.00
32.48


ATOM
13
O
VAL
A
2
−28.086
25.109
−22.245
1.00
31.10


ATOM
14
N
ASP
A
3
−30.160
25.286
−21.381
1.00
31.43


ATOM
15
CA
ASP
A
3
−30.791
24.530
−22.457
1.00
31.38


ATOM
16
CB
ASP
A
3
−32.283
24.323
−22.190
1.00
32.17


ATOM
17
CG
ASP
A
3
−32.522
23.492
−20.943
1.00
35.28


ATOM
18
OD1
ASP
A
3
−32.413
22.251
−21.028
1.00
36.80


ATOM
19
OD2
ASP
A
3
−32.786
24.092
−19.870
1.00
40.63


ATOM
20
C
ASP
A
3
−30.556
25.153
−23.818
1.00
30.59


ATOM
21
O
ASP
A
3
−30.282
24.446
−24.778
1.00
30.19


ATOM
22
N
ASP
A
4
−30.644
26.477
−23.875
1.00
29.89


ATOM
23
CA
ASP
A
4
−30.369
27.244
−25.083
1.00
29.99


ATOM
24
CB
ASP
A
4
−30.601
28.731
−24.822
1.00
31.12


ATOM
25
CG
ASP
A
4
−32.088
29.121
−24.785
1.00
34.16


ATOM
26
OD1
ASP
A
4
−32.991
28.260
−24.925
1.00
36.06


ATOM
27
OD2
ASP
A
4
−32.340
30.332
−24.608
1.00
39.96


ATOM
28
C
ASP
A
4
−28.925
27.049
−25.579
1.00
28.65


ATOM
29
O
ASP
A
4
−28.697
26.881
−26.770
1.00
28.51


ATOM
30
N
PHE
A
5
−27.961
27.096
−24.660
1.00
26.74


ATOM
31
CA
PHE
A
5
−26.553
26.860
−24.994
1.00
25.21


ATOM
32
CB
PHE
A
5
−25.666
27.110
−23.764
1.00
25.59


ATOM
33
CG
PHE
A
5
−24.244
26.646
−23.931
1.00
26.03


ATOM
34
CD1
PHE
A
5
−23.395
27.259
−24.854
1.00
27.29


ATOM
35
CE1
PHE
A
5
−22.063
26.823
−25.009
1.00
27.33


ATOM
36
CZ
PHE
A
5
−21.593
25.783
−24.228
1.00
26.77


ATOM
37
CE2
PHE
A
5
−22.425
25.181
−23.286
1.00
28.42


ATOM
38
CD2
PHE
A
5
−23.749
25.617
−23.144
1.00
28.42


ATOM
39
C
PHE
A
5
−26.352
25.438
−25.539
1.00
24.23


ATOM
40
O
PHE
A
5
−25.659
25.244
−26.544
1.00
23.56


ATOM
41
N
ILE
A
6
−26.974
24.458
−24.892
1.00
22.71


ATOM
42
CA
ILE
A
6
−26.835
23.065
−25.312
1.00
22.36


ATOM
43
CB
ILE
A
6
−27.491
22.106
−24.299
1.00
21.86


ATOM
44
CG1
ILE
A
6
−26.744
22.181
−22.956
1.00
22.27


ATOM
45
CD1
ILE
A
6
−27.384
21.347
−21.834
1.00
22.36


ATOM
46
CG2
ILE
A
6
−27.571
20.669
−24.848
1.00
21.69


ATOM
47
C
ILE
A
6
−27.388
22.855
−26.723
1.00
22.84


ATOM
48
O
ILE
A
6
−26.753
22.216
−27.573
1.00
21.76


ATOM
49
N
SER
A
7
−28.556
23.420
−26.996
1.00
23.10


ATOM
50
CA
SER
A
7
−29.146
23.175
−28.309
1.00
23.90


ATOM
51
CB
SER
A
7
−30.627
23.570
−28.320
1.00
25.04


ATOM
52
OG
SER
A
7
−30.717
24.982
−28.282
1.00
30.08


ATOM
53
C
SER
A
7
−28.340
23.874
−29.422
1.00
22.78


ATOM
54
O
SER
A
7
−28.186
23.337
−30.508
1.00
22.94


ATOM
55
N
THR
A
8
−27.800
25.053
−29.140
1.00
22.50


ATOM
56
CA
THR
A
8
−26.984
25.780
−30.115
1.00
23.05


ATOM
57
CB
THR
A
8
−26.834
27.247
−29.698
1.00
23.65


ATOM
58
OG1
THR
A
8
−28.138
27.839
−29.700
1.00
27.60


ATOM
59
CG2
THR
A
8
−25.939
28.018
−30.660
1.00
26.76


ATOM
60
C
THR
A
8
−25.601
25.159
−30.307
1.00
21.46


ATOM
61
O
THR
A
8
−25.109
25.051
−31.437
1.00
21.38


ATOM
62
N
GLU
A
9
−24.978
24.768
−29.200
1.00
19.11


ATOM
63
CA
GLU
A
9
−23.596
24.269
−29.243
1.00
18.01


ATOM
64
CB
GLU
A
9
−22.959
24.334
−27.847
1.00
17.76


ATOM
65
CG
GLU
A
9
−21.449
23.945
−27.794
1.00
17.71


ATOM
66
CD
GLU
A
9
−20.536
24.892
−28.609
1.00
20.86


ATOM
67
OE1
GLU
A
9
−20.949
26.010
−28.971
1.00
19.89


ATOM
68
OE2
GLU
A
9
−19.389
24.500
−28.909
1.00
19.22


ATOM
69
C
GLU
A
9
−23.462
22.846
−29.784
1.00
17.77


ATOM
70
O
GLU
A
9
−22.423
22.505
−30.368
1.00
18.05


ATOM
71
N
THR
A
10
−24.485
22.020
−29.593
1.00
15.87


ATOM
72
CA
THR
A
10
−24.404
20.609
−29.958
1.00
17.31


ATOM
73
CB
THR
A
10
−25.677
19.823
−29.525
1.00
17.46


ATOM
74
OG1
THR
A
10
−25.768
19.860
−28.090
1.00
17.46


ATOM
75
CG2
THR
A
10
−25.616
18.374
−30.037
1.00
18.42


ATOM
76
C
THR
A
10
−24.026
20.346
−31.430
1.00
17.40


ATOM
77
O
THR
A
10
−23.073
19.615
−31.682
1.00
17.22


ATOM
78
N
PRO
A
11
−24.764
20.934
−32.412
1.00
18.30


ATOM
79
CA
PRO
A
11
−24.346
20.649
−33.798
1.00
18.11


ATOM
80
CB
PRO
A
11
−25.440
21.317
−34.662
1.00
18.57


ATOM
81
CG
PRO
A
11
−26.094
22.310
−33.771
1.00
19.16


ATOM
82
CD
PRO
A
11
−25.975
21.779
−32.361
1.00
18.54


ATOM
83
C
PRO
A
11
−22.963
21.231
−34.142
1.00
17.81


ATOM
84
O
PRO
A
11
−22.241
20.655
−34.964
1.00
17.74


ATOM
85
N
ILE
A
12
−22.601
22.353
−33.520
1.00
16.85


ATOM
86
CA
ILE
A
12
−21.279
22.936
−33.731
1.00
16.66


ATOM
87
CB
ILE
A
12
−21.161
24.319
−33.112
1.00
17.25


ATOM
88
CG1
ILE
A
12
−22.194
25.267
−33.751
1.00
19.25


ATOM
89
CD1
ILE
A
12
−22.289
26.635
−33.101
1.00
21.45


ATOM
90
CG2
ILE
A
12
−19.714
24.855
−33.270
1.00
18.75


ATOM
91
C
ILE
A
12
−20.170
22.023
−33.178
1.00
16.30


ATOM
92
O
ILE
A
12
−19.155
21.798
−33.848
1.00
14.64


ATOM
93
N
ALA
A
13
−20.360
21.527
−31.951
1.00
15.28


ATOM
94
CA
ALA
A
13
−19.375
20.627
−31.304
1.00
15.19


ATOM
95
CB
ALA
A
13
−19.788
20.332
−29.883
1.00
15.31


ATOM
96
C
ALA
A
13
−19.204
19.326
−32.092
1.00
15.37


ATOM
97
O
ALA
A
13
−18.083
18.834
−32.297
1.00
13.56


ATOM
98
N
LEU
A
14
−20.320
18.743
−32.531
1.00
15.13


ATOM
99
CA
LEU
A
14
−20.225
17.503
−33.285
1.00
16.06


ATOM
100
CB
LEU
A
14
−21.630
16.921
−33.510
1.00
17.33


ATOM
101
CG
LEU
A
14
−21.689
15.563
−34.212
1.00
20.02


ATOM
102
CD1
LEU
A
14
−20.946
14.460
−33.471
1.00
23.09


ATOM
103
CD2
LEU
A
14
−23.150
15.225
−34.390
1.00
21.86


ATOM
104
C
LEU
A
14
−19.506
17.749
−34.623
1.00
15.61


ATOM
105
O
LEU
A
14
−18.651
16.947
−35.039
1.00
14.82


ATOM
106
N
ASN
A
15
−19.853
18.852
−35.285
1.00
15.30


ATOM
107
CA
ASN
A
15
−19.236
19.228
−36.567
1.00
16.34


ATOM
108
CB
ASN
A
15
−19.848
20.545
−37.073
1.00
16.07


ATOM
109
CG
ASN
A
15
−19.232
21.010
−38.388
1.00
18.31


ATOM
110
OD1
ASN
A
15
−19.565
20.487
−39.431
1.00
17.60


ATOM
111
ND2
ASN
A
15
−18.312
21.987
−38.325
1.00
21.40


ATOM
112
C
ASN
A
15
−17.736
19.450
−36.405
1.00
15.35


ATOM
113
O
ASN
A
15
−16.926
18.954
−37.198
1.00
15.29


ATOM
114
N
ASN
A
16
−17.385
20.180
−35.355
1.00
14.82


ATOM
115
CA
ASN
A
16
−15.992
20.555
−35.144
1.00
15.27


ATOM
116
CB
ASN
A
16
−15.872
21.693
−34.148
1.00
15.41


ATOM
117
CG
ASN
A
16
−16.276
23.023
−34.737
1.00
16.53


ATOM
118
OD1
ASN
A
16
−16.517
23.136
−35.954
1.00
18.08


ATOM
119
ND2
ASN
A
16
−16.326
24.050
−33.896
1.00
16.35


ATOM
120
C
ASN
A
16
−15.159
19.362
−34.723
1.00
14.91


ATOM
121
O
ASN
A
16
−13.975
19.261
−35.099
1.00
15.34


ATOM
122
N
LEU
A
17
−15.771
18.460
−33.956
1.00
14.29


ATOM
123
CA
LEU
A
17
−15.114
17.191
−33.610
1.00
13.90


ATOM
124
CB
LEU
A
17
−16.003
16.346
−32.672
1.00
13.94


ATOM
125
CG
LEU
A
17
−15.351
15.065
−32.133
1.00
16.81


ATOM
126
CD1
LEU
A
17
−15.933
14.708
−30.779
1.00
20.06


ATOM
127
CD2
LEU
A
17
−15.484
13.880
−33.097
1.00
19.31


ATOM
128
C
LEU
A
17
−14.763
16.409
−34.880
1.00
14.06


ATOM
129
O
LEU
A
17
−13.613
15.957
−35.073
1.00
12.69


ATOM
130
N
LEU
A
18
−15.774
16.215
−35.730
1.00
13.19


ATOM
131
CA
LEU
A
18
−15.589
15.441
−36.957
1.00
14.25


ATOM
132
CB
LEU
A
18
−16.952
15.027
−37.545
1.00
13.56


ATOM
133
CG
LEU
A
18
−17.717
14.013
−36.684
1.00
16.49


ATOM
134
CD1
LEU
A
18
−19.171
13.874
−37.165
1.00
16.33


ATOM
135
CD2
LEU
A
18
−17.020
12.647
−36.655
1.00
18.51


ATOM
136
C
LEU
A
18
−14.703
16.132
−38.007
1.00
13.49


ATOM
137
O
LEU
A
18
−14.083
15.435
−38.820
1.00
14.69


ATOM
138
N
CYS
A
19
−14.613
17.462
−37.964
1.00
13.01


ATOM
139
CA
CYS
A
19
−13.629
18.223
−38.760
1.00
13.22


ATOM
140
CB
CYS
A
19
−13.796
19.738
−38.556
1.00
14.20


ATOM
141
SG
CYS
A
19
−15.125
20.407
−39.642
1.00
16.22


ATOM
142
C
CYS
A
19
−12.182
17.808
−38.450
1.00
13.86


ATOM
143
O
CYS
A
19
−11.278
18.043
−39.259
1.00
13.42


ATOM
144
N
ASN
A
20
−11.968
17.219
−37.272
1.00
13.21


ATOM
145
CA
ASN
A
20
−10.594
16.850
−36.830
1.00
13.62


ATOM
146
CB
ASN
A
20
−10.394
17.184
−35.324
1.00
13.52


ATOM
147
CG
ASN
A
20
−10.242
18.687
−35.055
1.00
16.17


ATOM
148
OD1
ASN
A
20
−10.035
19.119
−33.897
1.00
17.34


ATOM
149
ND2
ASN
A
20
−10.343
19.486
−36.090
1.00
11.87


ATOM
150
C
ASN
A
20
−10.262
15.381
−37.116
1.00
13.99


ATOM
151
O
ASN
A
20
−9.238
14.857
−36.646
1.00
14.28


ATOM
152
N
VAL
A
21
−11.123
14.705
−37.875
1.00
13.41


ATOM
153
CA
VAL
A
21
−10.923
13.287
−38.167
1.00
14.20


ATOM
154
CB
VAL
A
21
−12.177
12.448
−37.827
1.00
14.30


ATOM
155
CG1
VAL
A
21
−11.953
10.971
−38.189
1.00
15.30


ATOM
156
CG2
VAL
A
21
−12.517
12.553
−36.312
1.00
14.17


ATOM
157
C
VAL
A
21
−10.551
13.136
−39.644
1.00
14.35


ATOM
158
O
VAL
A
21
−11.255
13.642
−40.491
1.00
15.68


ATOM
159
N
GLY
A
22
−9.461
12.449
−39.953
1.00
15.67


ATOM
160
CA
GLY
A
22
−9.061
12.300
−41.377
1.00
15.70


ATOM
161
C
GLY
A
22
−9.843
11.182
−42.060
1.00
17.34


ATOM
162
O
GLY
A
22
−10.453
10.358
−41.397
1.00
17.15


ATOM
163
N
PRO
A
23
−9.806
11.117
−43.404
1.00
18.42


ATOM
164
CA
PRO
A
23
−9.009
11.946
−44.278
1.00
18.20


ATOM
165
CB
PRO
A
23
−8.716
10.990
−45.446
1.00
18.64


ATOM
166
CG
PRO
A
23
−9.983
10.171
−45.560
1.00
18.81


ATOM
167
CD
PRO
A
23
−10.568
10.092
−44.153
1.00
18.59


ATOM
168
C
PRO
A
23
−9.761
13.182
−44.753
1.00
19.05


ATOM
169
O
PRO
A
23
−9.183
14.055
−45.426
1.00
19.36


ATOM
170
N
ASP
A
24
−11.034
13.288
−44.385
1.00
18.76


ATOM
171
CA
ASP
A
24
−11.878
14.305
−44.996
1.00
19.39


ATOM
172
CB
ASP
A
24
−13.015
13.636
−45.781
1.00
20.87


ATOM
173
CG
ASP
A
24
−13.920
12.784
−44.913
1.00
24.34


ATOM
174
OD1
ASP
A
24
−13.502
12.291
−43.839
1.00
27.70


ATOM
175
OD2
ASP
A
24
−15.079
12.580
−45.330
1.00
28.83


ATOM
176
C
ASP
A
24
−12.452
15.372
−44.061
1.00
18.08


ATOM
177
O
ASP
A
24
−13.208
16.245
−44.509
1.00
17.78


ATOM
178
N
GLY
A
25
−12.100
15.331
−42.775
1.00
16.65


ATOM
179
CA
GLY
A
25
−12.634
16.343
−41.852
1.00
16.02


ATOM
180
C
GLY
A
25
−12.152
17.718
−42.292
1.00
15.70


ATOM
181
O
GLY
A
25
−11.033
17.849
−42.811
1.00
16.22


ATOM
182
N
CYS
A
26
−12.979
18.752
−42.086
1.00
15.10


ATOM
183
CA
CYS
A
26
−12.698
20.078
−42.637
1.00
15.46


ATOM
184
CB
CYS
A
26
−13.899
21.037
−42.475
1.00
15.47


ATOM
185
SG
CYS
A
26
−14.147
21.739
−40.786
1.00
16.91


ATOM
186
C
CYS
A
26
−11.407
20.731
−42.116
1.00
15.65


ATOM
187
O
CYS
A
26
−10.896
21.643
−42.763
1.00
15.80


ATOM
188
N
ARG
A
27
−10.879
20.259
−40.973
1.00
15.02


ATOM
189
CA
ARG
A
27
−9.615
20.808
−40.443
1.00
14.56


ATOM
190
CB
ARG
A
27
−9.819
21.480
−39.066
1.00
15.00


ATOM
191
CG
ARG
A
27
−10.695
22.728
−39.164
1.00
15.15


ATOM
192
CD
ARG
A
27
−10.826
23.551
−37.888
1.00
14.30


ATOM
193
NE
ARG
A
27
−11.874
24.566
−38.080
1.00
15.05


ATOM
194
CZ
ARG
A
27
−13.160
24.420
−37.761
1.00
17.96


ATOM
195
NH1
ARG
A
27
−13.623
23.293
−37.211
1.00
17.37


ATOM
196
NH2
ARG
A
27
−14.009
25.415
−38.025
1.00
19.55


ATOM
197
C
ARG
A
27
−8.489
19.776
−40.394
1.00
15.49


ATOM
198
O
ARG
A
27
−7.389
20.079
−39.888
1.00
15.40


ATOM
199
N
ALA
A
28
−8.768
18.577
−40.910
1.00
15.17


ATOM
200
CA
ALA
A
28
−7.805
17.484
−40.988
1.00
16.06


ATOM
201
CB
ALA
A
28
−8.163
16.374
−39.975
1.00
15.52


ATOM
202
C
ALA
A
28
−7.744
16.913
−42.394
1.00
16.96


ATOM
203
O
ALA
A
28
−7.453
15.730
−42.581
1.00
17.60


ATOM
204
N
PHE
A
29
−8.028
17.756
−43.380
1.00
17.45


ATOM
205
CA
PHE
A
29
−8.188
17.272
−44.744
1.00
18.68


ATOM
206
CB
PHE
A
29
−8.728
18.376
−45.636
1.00
19.45


ATOM
207
CG
PHE
A
29
−9.299
17.864
−46.919
1.00
20.86


ATOM
208
CD1
PHE
A
29
−8.515
17.827
−48.071
1.00
23.76


ATOM
209
CE1
PHE
A
29
−9.042
17.343
−49.267
1.00
25.46


ATOM
210
CZ
PHE
A
29
−10.357
16.889
−49.318
1.00
22.85


ATOM
211
CE2
PHE
A
29
−11.151
16.924
−48.180
1.00
24.78


ATOM
212
CD2
PHE
A
29
−10.611
17.408
−46.973
1.00
22.71


ATOM
213
C
PHE
A
29
−6.853
16.783
−45.296
1.00
19.10


ATOM
214
O
PHE
A
29
−5.862
17.501
−45.224
1.00
19.40


ATOM
215
N
GLY
A
30
−6.830
15.558
−45.816
1.00
18.73


ATOM
216
CA
GLY
A
30
−5.603
15.008
−46.398
1.00
19.00


ATOM
217
C
GLY
A
30
−4.717
14.307
−45.399
1.00
19.69


ATOM
218
O
GLY
A
30
−3.657
13.809
−45.768
1.00
19.61


ATOM
219
N
THR
A
31
−5.133
14.255
−44.123
1.00
18.86


ATOM
220
CA
THR
A
31
−4.450
13.384
−43.165
1.00
19.14


ATOM
221
CB
THR
A
31
−4.846
13.689
−41.689
1.00
18.79


ATOM
222
OG1
THR
A
31
−6.265
13.579
−41.559
1.00
18.61


ATOM
223
CG2
THR
A
31
−4.410
15.106
−41.262
1.00
16.47


ATOM
224
C
THR
A
31
−4.812
11.925
−43.498
1.00
19.11


ATOM
225
O
THR
A
31
−5.713
11.661
−44.313
1.00
19.69


ATOM
226
N
SER
A
32
−4.107
10.982
−42.881
1.00
19.74


ATOM
227
CA
SER
A
32
−4.367
9.554
−43.094
1.00
20.00


ATOM
228
CB
SER
A
32
−3.411
8.722
−42.248
1.00
20.73


ATOM
229
OG
SER
A
32
−2.064
8.973
−42.612
1.00
21.56


ATOM
230
C
SER
A
32
−5.806
9.217
−42.704
1.00
20.57


ATOM
231
O
SER
A
32
−6.334
9.778
−41.732
1.00
20.70


ATOM
232
N
ALA
A
33
−6.443
8.319
−43.452
1.00
19.94


ATOM
233
CA
ALA
A
33
−7.768
7.823
−43.068
1.00
19.61


ATOM
234
CB
ALA
A
33
−8.232
6.729
−44.035
1.00
19.31


ATOM
235
C
ALA
A
33
−7.764
7.285
−41.637
1.00
19.10


ATOM
236
O
ALA
A
33
−6.906
6.482
−41.264
1.00
19.49


ATOM
237
N
GLY
A
34
−8.742
7.719
−40.856
1.00
17.74


ATOM
238
CA
GLY
A
34
−8.878
7.282
−39.473
1.00
18.31


ATOM
239
C
GLY
A
34
−7.988
8.020
−38.473
1.00
18.48


ATOM
240
O
GLY
A
34
−8.050
7.739
−37.271
1.00
19.07


ATOM
241
N
ALA
A
35
−7.173
8.959
−38.937
1.00
17.05


ATOM
242
CA
ALA
A
35
−6.329
9.723
−38.000
1.00
17.17


ATOM
243
CB
ALA
A
35
−5.167
10.376
−38.730
1.00
17.10


ATOM
244
C
ALA
A
35
−7.173
10.784
−37.271
1.00
16.55


ATOM
245
O
ALA
A
35
−8.174
11.271
−37.808
1.00
17.35


ATOM
246
N
VAL
A
36
−6.793
11.130
−36.051
1.00
15.39


ATOM
247
CA
VAL
A
36
−7.490
12.198
−35.328
1.00
14.41


ATOM
248
CB
VAL
A
36
−8.142
11.687
−34.031
1.00
15.02


ATOM
249
CG1
VAL
A
36
−8.903
12.828
−33.349
1.00
16.72


ATOM
250
CG2
VAL
A
36
−9.081
10.520
−34.336
1.00
16.45


ATOM
251
C
VAL
A
36
−6.407
13.201
−34.944
1.00
14.36


ATOM
252
O
VAL
A
36
−5.421
12.816
−34.311
1.00
14.44


ATOM
253
N
ILE
A
37
−6.566
14.454
−35.331
1.00
13.68


ATOM
254
CA
ILE
A
37
−5.614
15.470
−34.893
1.00
13.67


ATOM
255
CB
ILE
A
37
−5.528
16.687
−35.849
1.00
13.66


ATOM
256
CG1
ILE
A
37
−6.847
17.486
−35.901
1.00
14.31


ATOM
257
CD1
ILE
A
37
−6.773
18.712
−36.864
1.00
14.21


ATOM
258
CG2
ILE
A
37
−5.158
16.214
−37.260
1.00
14.62


ATOM
259
C
ILE
A
37
−6.041
15.908
−33.505
1.00
13.27


ATOM
260
O
ILE
A
37
−7.235
16.011
−33.224
1.00
12.99


ATOM
261
N
ALA
A
38
−5.081
16.159
−32.630
1.00
13.03


ATOM
262
CA
ALA
A
38
−5.445
16.697
−31.333
1.00
12.81


ATOM
263
CB
ALA
A
38
−4.235
16.680
−30.377
1.00
12.73


ATOM
264
C
ALA
A
38
−6.046
18.122
−31.497
1.00
12.45


ATOM
265
O
ALA
A
38
−6.939
18.503
−30.775
1.00
12.23


ATOM
266
N
SER
A
39
−5.555
18.870
−32.482
1.00
12.90


ATOM
267
CA
SER
A
39
−5.973
20.246
−32.769
1.00
12.85


ATOM
268
CB
SER
A
39
−5.512
21.211
−31.657
1.00
12.63


ATOM
269
OG
SER
A
39
−5.312
22.563
−32.108
1.00
12.57


ATOM
270
C
SER
A
39
−5.281
20.593
−34.090
1.00
13.33


ATOM
271
O
SER
A
39
−4.215
20.043
−34.376
1.00
13.48


ATOM
272
N
PRO
A
40
−5.880
21.500
−34.885
1.00
13.12


ATOM
273
CA
PRO
A
40
−5.248
21.999
−36.108
1.00
13.76


ATOM
274
CB
PRO
A
40
−6.407
22.689
−36.860
1.00
14.41


ATOM
275
CG
PRO
A
40
−7.386
23.045
−35.797
1.00
14.32


ATOM
276
CD
PRO
A
40
−7.223
22.081
−34.665
1.00
13.18


ATOM
277
C
PRO
A
40
−4.126
23.010
−35.860
1.00
14.27


ATOM
278
O
PRO
A
40
−3.474
23.420
−36.824
1.00
14.43


ATOM
279
N
SER
A
41
−3.864
23.381
−34.599
1.00
13.42


ATOM
280
CA
SER
A
41
−2.799
24.336
−34.318
1.00
14.56


ATOM
281
CB
SER
A
41
−2.788
24.817
−32.840
1.00
14.40


ATOM
282
OG
SER
A
41
−3.962
25.574
−32.534
1.00
16.91


ATOM
283
C
SER
A
41
−1.446
23.713
−34.676
1.00
14.51


ATOM
284
O
SER
A
41
−1.123
22.626
−34.218
1.00
13.96


ATOM
285
N
THR
A
42
−0.650
24.433
−35.470
1.00
15.63


ATOM
286
CA
THR
A
42
0.652
23.924
−35.919
1.00
16.17


ATOM
287
CB
THR
A
42
0.750
23.997
−37.458
1.00
17.02


ATOM
288
OG1
THR
A
42
0.267
25.283
−37.890
1.00
17.03


ATOM
289
CG2
THR
A
42
−0.110
22.906
−38.078
1.00
16.03


ATOM
290
C
THR
A
42
1.814
24.732
−35.322
1.00
17.44


ATOM
291
O
THR
A
42
2.967
24.297
−35.382
1.00
17.10


ATOM
292
N
ILE
A
43
1.509
25.913
−34.787
1.00
18.37


ATOM
293
CA
ILE
A
43
2.510
26.786
−34.171
1.00
20.62


ATOM
294
CB
ILE
A
43
2.923
27.952
−35.114
1.00
20.73


ATOM
295
CG1
ILE
A
43
3.550
27.428
−36.411
1.00
21.88


ATOM
296
CD1
ILE
A
43
3.788
28.507
−37.508
1.00
22.99


ATOM
297
CG2
ILE
A
43
3.895
28.910
−34.409
1.00
21.41


ATOM
298
C
ILE
A
43
1.908
27.395
−32.935
1.00
21.00


ATOM
299
O
ILE
A
43
0.796
27.921
−32.995
1.00
21.76


ATOM
300
N
ASP
A
44
2.683
27.470
−31.874
1.00
21.61


ATOM
301
CA
ASP
A
44
2.237
27.975
−30.572
1.00
23.04


ATOM
302
CB
ASP
A
44
2.408
29.506
−30.492
1.00
24.75


ATOM
303
CG
ASP
A
44
2.170
30.064
−29.098
1.00
31.28


ATOM
304
OD1
ASP
A
44
2.362
29.340
−28.094
1.00
37.92


ATOM
305
OD2
ASP
A
44
1.766
31.260
−28.997
1.00
40.00


ATOM
306
C
ASP
A
44
0.782
27.608
−30.196
1.00
21.65


ATOM
307
O
ASP
A
44
−0.046
28.502
−29.981
1.00
22.69


ATOM
308
N
PRO
A
45
0.441
26.449
−29.805
1.00
19.86


ATOM
309
CA
PRO
A
45
1.356
25.320
−29.775
1.00
18.66


ATOM
310
CB
PRO
A
45
0.883
24.549
−28.549
1.00
18.40


ATOM
311
CG
PRO
A
45
−0.653
24.763
−28.586
1.00
18.13


ATOM
312
CD
PRO
A
45
−0.899
26.066
−29.318
1.00
20.04


ATOM
313
C
PRO
A
45
1.253
24.454
−31.026
1.00
17.74


ATOM
314
O
PRO
A
45
0.368
24.652
−31.858
1.00
17.36


ATOM
315
N
ASP
A
46
2.178
23.512
−31.160
1.00
15.95


ATOM
316
CA
ASP
A
46
2.124
22.573
−32.275
1.00
14.75


ATOM
317
CB
ASP
A
46
3.551
22.255
−32.738
1.00
14.59


ATOM
318
CG
ASP
A
46
3.601
21.161
−33.818
1.00
16.17


ATOM
319
OD1
ASP
A
46
2.543
20.787
−34.389
1.00
15.61


ATOM
320
OD2
ASP
A
46
4.712
20.641
−34.054
1.00
20.18


ATOM
321
C
ASP
A
46
1.436
21.303
−31.748
1.00
13.83


ATOM
322
O
ASP
A
46
2.081
20.489
−31.089
1.00
13.59


ATOM
323
N
TYR
A
47
0.126
21.165
−31.992
1.00
11.86


ATOM
324
CA
TYR
A
47
−0.621
19.975
−31.580
1.00
12.11


ATOM
325
CB
TYR
A
47
−1.895
20.387
−30.854
1.00
11.69


ATOM
326
CG
TYR
A
47
−1.773
20.722
−29.377
1.00
12.59


ATOM
327
CD1
TYR
A
47
−0.589
21.236
−28.827
1.00
13.54


ATOM
328
CE1
TYR
A
47
−0.524
21.586
−27.462
1.00
12.81


ATOM
329
CZ
TYR
A
47
−1.652
21.407
−26.673
1.00
13.40


ATOM
330
OH
TYR
A
47
−1.620
21.755
−25.354
1.00
13.08


ATOM
331
CE2
TYR
A
47
−2.825
20.887
−27.208
1.00
12.02


ATOM
332
CD2
TYR
A
47
−2.876
20.540
−28.532
1.00
12.76


ATOM
333
C
TYR
A
47
−0.994
19.090
−32.772
1.00
11.46


ATOM
334
O
TYR
A
47
−1.885
18.239
−32.692
1.00
11.66


ATOM
335
N
TYR
A
48
−0.316
19.301
−33.893
1.00
12.26


ATOM
336
CA
TYR
A
48
−0.697
18.639
−35.132
1.00
12.80


ATOM
337
CB
TYR
A
48
−0.323
19.509
−36.348
1.00
12.75


ATOM
338
CG
TYR
A
48
−1.134
19.146
−37.569
1.00
13.21


ATOM
339
CD1
TYR
A
48
−2.492
19.482
−37.652
1.00
14.46


ATOM
340
CE1
TYR
A
48
−3.254
19.124
−38.767
1.00
15.86


ATOM
341
CZ
TYR
A
48
−2.643
18.453
−39.823
1.00
14.62


ATOM
342
OH
TYR
A
48
−3.390
18.106
−40.936
1.00
16.20


ATOM
343
CE2
TYR
A
48
−1.295
18.086
−39.756
1.00
15.86


ATOM
344
CD2
TYR
A
48
−0.543
18.456
−38.638
1.00
13.44


ATOM
345
C
TYR
A
48
−0.072
17.245
−35.187
1.00
13.47


ATOM
346
O
TYR
A
48
0.877
16.986
−35.940
1.00
13.95


ATOM
347
N
TYR
A
49
−0.592
16.360
−34.338
1.00
13.13


ATOM
348
CA
TYR
A
49
−0.131
14.987
−34.171
1.00
13.51


ATOM
349
CB
TYR
A
49
0.887
14.842
−33.009
1.00
13.11


ATOM
350
CG
TYR
A
49
2.133
15.662
−33.216
1.00
13.90


ATOM
351
CD1
TYR
A
49
3.193
15.174
−33.996
1.00
13.54


ATOM
352
CE1
TYR
A
49
4.354
15.964
−34.216
1.00
13.41


ATOM
353
CZ
TYR
A
49
4.419
17.225
−33.665
1.00
14.69


ATOM
354
OH
TYR
A
49
5.511
18.016
−33.883
1.00
17.21


ATOM
355
CE2
TYR
A
49
3.365
17.737
−32.906
1.00
13.49


ATOM
356
CD2
TYR
A
49
2.227
16.952
−32.698
1.00
13.78


ATOM
357
C
TYR
A
49
−1.349
14.181
−33.783
1.00
13.93


ATOM
358
O
TYR
A
49
−2.390
14.759
−33.406
1.00
13.00


ATOM
359
N
MET
A
50
−1.203
12.857
−33.839
1.00
13.66


ATOM
360
CA
MET
A
50
−2.241
11.940
−33.365
1.00
14.56


ATOM
361
CB
MET
A
50
−2.447
10.822
−34.381
1.00
15.21


ATOM
362
CG
MET
A
50
−3.532
9.811
−33.947
1.00
15.64


ATOM
363
SD
MET
A
50
−3.996
8.804
−35.361
1.00
19.52


ATOM
364
CE
MET
A
50
−5.204
7.742
−34.566
1.00
17.12


ATOM
365
C
MET
A
50
−1.797
11.323
−32.060
1.00
14.38


ATOM
366
O
MET
A
50
−0.806
10.583
−32.024
1.00
13.80


ATOM
367
N
TRP
A
51
−2.528
11.608
−30.984
1.00
13.47


ATOM
368
CA
TRP
A
51
−2.265
10.965
−29.720
1.00
13.13


ATOM
369
CB
TRP
A
51
−2.598
11.930
−28.585
1.00
12.85


ATOM
370
CG
TRP
A
51
−1.478
12.809
−28.116
1.00
13.64


ATOM
371
CD1
TRP
A
51
−0.671
12.604
−27.011
1.00
13.49


ATOM
372
NE1
TRP
A
51
0.211
13.657
−26.864
1.00
12.36


ATOM
373
CE2
TRP
A
51
−0.023
14.573
−27.858
1.00
11.83


ATOM
374
CD2
TRP
A
51
−1.076
14.065
−28.674
1.00
13.12


ATOM
375
CE3
TRP
A
51
−1.506
14.825
−29.772
1.00
11.07


ATOM
376
CZ3
TRP
A
51
−0.859
16.061
−30.035
1.00
12.87


ATOM
377
CH2
TRP
A
51
0.193
16.522
−29.218
1.00
13.24


ATOM
378
CZ2
TRP
A
51
0.618
15.806
−28.127
1.00
12.61


ATOM
379
C
TRP
A
51
−3.136
9.732
−29.575
1.00
13.35


ATOM
380
O
TRP
A
51
−4.322
9.769
−29.907
1.00
12.89


ATOM
381
N
THR
A
52
−2.576
8.652
−29.024
1.00
13.20


ATOM
382
CA
THR
A
52
−3.386
7.462
−28.753
1.00
13.02


ATOM
383
CB
THR
A
52
−2.520
6.300
−28.235
1.00
13.66


ATOM
384
OG1
THR
A
52
−1.553
5.999
−29.246
1.00
15.07


ATOM
385
CG2
THR
A
52
−3.341
5.026
−27.952
1.00
12.21


ATOM
386
C
THR
A
52
−4.533
7.807
−27.800
1.00
12.48


ATOM
387
O
THR
A
52
−5.670
7.402
−28.034
1.00
12.78


ATOM
388
N
ARG
A
53
−4.224
8.556
−26.747
1.00
12.03


ATOM
389
CA
ARG
A
53
−5.238
8.868
−25.737
1.00
11.89


ATOM
390
CB
ARG
A
53
−4.607
9.570
−24.545
1.00
11.46


ATOM
391
CG
ARG
A
53
−5.611
10.330
−23.618
1.00
13.19


ATOM
392
CD
ARG
A
53
−4.896
10.881
−22.375
1.00
11.14


ATOM
393
NE
ARG
A
53
−3.793
11.694
−22.819
1.00
12.52


ATOM
394
CZ
ARG
A
53
−2.509
11.330
−22.769
1.00
13.67


ATOM
395
NH1
ARG
A
53
−2.148
10.182
−22.180
1.00
13.97


ATOM
396
NH2
ARG
A
53
−1.590
12.151
−23.270
1.00
13.05


ATOM
397
C
ARG
A
53
−6.395
9.709
−26.319
1.00
12.45


ATOM
398
O
ARG
A
53
−7.558
9.289
−26.244
1.00
11.74


ATOM
399
N
ASP
A
54
−6.090
10.885
−26.874
1.00
11.73


ATOM
400
CA
ASP
A
54
−7.169
11.747
−27.385
1.00
11.90


ATOM
401
CB
ASP
A
54
−6.638
13.018
−28.053
1.00
12.25


ATOM
402
CG
ASP
A
54
−5.794
13.879
−27.120
1.00
13.97


ATOM
403
OD1
ASP
A
54
−4.983
13.332
−26.354
1.00
13.57


ATOM
404
OD2
ASP
A
54
−5.910
15.110
−27.215
1.00
13.88


ATOM
405
C
ASP
A
54
−8.002
11.005
−28.420
1.00
12.00


ATOM
406
O
ASP
A
54
−9.236
11.138
−28.454
1.00
10.97


ATOM
407
N
SER
A
55
−7.334
10.250
−29.297
1.00
11.19


ATOM
408
CA
SER
A
55
−8.034
9.544
−30.388
1.00
12.36


ATOM
409
CB
SER
A
55
−7.017
8.901
−31.340
1.00
13.05


ATOM
410
OG
SER
A
55
−6.171
9.930
−31.882
1.00
14.23


ATOM
411
C
SER
A
55
−8.996
8.489
−29.838
1.00
12.57


ATOM
412
O
SER
A
55
−10.130
8.348
−30.327
1.00
12.76


ATOM
413
N
ALA
A
56
−8.556
7.764
−28.819
1.00
12.60


ATOM
414
CA
ALA
A
56
−9.373
6.718
−28.218
1.00
13.25


ATOM
415
CB
ALA
A
56
−8.517
5.830
−27.329
1.00
12.73


ATOM
416
C
ALA
A
56
−10.551
7.301
−27.415
1.00
13.85


ATOM
417
O
ALA
A
56
−11.640
6.723
−27.409
1.00
14.51


ATOM
418
N
LEU
A
57
−10.328
8.420
−26.723
1.00
14.23


ATOM
419
CA
LEU
A
57
−11.417
9.059
−25.954
1.00
13.95


ATOM
420
CB
LEU
A
57
−10.891
10.186
−25.060
1.00
14.45


ATOM
421
CG
LEU
A
57
−10.088
9.751
−23.834
1.00
14.89


ATOM
422
CD1
LEU
A
57
−9.507
11.013
−23.161
1.00
16.19


ATOM
423
CD2
LEU
A
57
−10.919
8.911
−22.867
1.00
16.02


ATOM
424
C
LEU
A
57
−12.483
9.609
−26.886
1.00
13.92


ATOM
425
O
LEU
A
57
−13.685
9.488
−26.627
1.00
13.70


ATOM
426
N
VAL
A
58
−12.027
10.199
−27.975
1.00
13.15


ATOM
427
CA
VAL
A
58
−12.920
10.751
−28.989
1.00
15.20


ATOM
428
CB
VAL
A
58
−12.133
11.605
−30.031
1.00
14.52


ATOM
429
CG1
VAL
A
58
−12.970
11.861
−31.302
1.00
17.86


ATOM
430
CG2
VAL
A
58
−11.704
12.954
−29.393
1.00
15.79


ATOM
431
C
VAL
A
58
−13.704
9.624
−29.655
1.00
15.20


ATOM
432
O
VAL
A
58
−14.930
9.718
−29.784
1.00
15.30


ATOM
433
N
PHE
A
59
−13.026
8.553
−30.058
1.00
15.02


ATOM
434
CA
PHE
A
59
−13.766
7.477
−30.713
1.00
15.23


ATOM
435
CB
PHE
A
59
−12.882
6.601
−31.582
1.00
15.78


ATOM
436
CG
PHE
A
59
−12.859
7.058
−33.003
1.00
15.10


ATOM
437
CD1
PHE
A
59
−11.872
7.927
−33.444
1.00
16.45


ATOM
438
CE1
PHE
A
59
−11.861
8.401
−34.768
1.00
19.37


ATOM
439
CZ
PHE
A
59
−12.876
8.026
−35.644
1.00
16.44


ATOM
440
CE2
PHE
A
59
−13.901
7.165
−35.186
1.00
16.90


ATOM
441
CD2
PHE
A
59
−13.895
6.709
−33.882
1.00
16.17


ATOM
442
C
PHE
A
59
−14.674
6.681
−29.785
1.00
15.69


ATOM
443
O
PHE
A
59
−15.699
6.175
−30.220
1.00
15.46


ATOM
444
N
LYS
A
60
−14.321
6.586
−28.510
1.00
15.65


ATOM
445
CA
LYS
A
60
−15.257
5.994
−27.552
1.00
16.61


ATOM
446
CB
LYS
A
60
−14.661
5.954
−26.144
1.00
16.44


ATOM
447
CG
LYS
A
60
−15.626
5.363
−25.059
1.00
17.65


ATOM
448
CD
LYS
A
60
−16.174
3.992
−25.433
1.00
18.35


ATOM
449
CE
LYS
A
60
−16.738
3.234
−24.199
1.00
19.79


ATOM
450
NZ
LYS
A
60
−17.819
3.976
−23.512
1.00
18.40


ATOM
451
C
LYS
A
60
−16.577
6.779
−27.579
1.00
16.72


ATOM
452
O
LYS
A
60
−17.663
6.182
−27.681
1.00
17.08


ATOM
453
N
ASN
A
61
−16.487
8.101
−27.508
1.00
16.77


ATOM
454
CA
ASN
A
61
−17.680
8.948
−27.628
1.00
18.06


ATOM
455
CB
ASN
A
61
−17.278
10.424
−27.573
1.00
19.41


ATOM
456
CG
ASN
A
61
−18.465
11.375
−27.643
1.00
22.93


ATOM
457
OD1
ASN
A
61
−19.585
11.057
−27.231
1.00
30.05


ATOM
458
ND2
ASN
A
61
−18.206
12.568
−28.130
1.00
29.54


ATOM
459
C
ASN
A
61
−18.480
8.659
−28.907
1.00
17.28


ATOM
460
O
ASN
A
61
−19.697
8.475
−28.852
1.00
18.11


ATOM
461
N
LEU
A
62
−17.799
8.647
−30.056
1.00
16.54


ATOM
462
CA
LEU
A
62
−18.460
8.379
−31.334
1.00
16.19


ATOM
463
CB
LEU
A
62
−17.479
8.572
−32.499
1.00
16.85


ATOM
464
CG
LEU
A
62
−17.047
10.027
−32.697
1.00
18.47


ATOM
465
CD1
LEU
A
62
−16.118
10.153
−33.916
1.00
20.38


ATOM
466
CD2
LEU
A
62
−18.263
10.925
−32.837
1.00
19.93


ATOM
467
C
LEU
A
62
−19.089
6.991
−31.371
1.00
16.01


ATOM
468
O
LEU
A
62
−20.225
6.833
−31.842
1.00
15.98


ATOM
469
N
ILE
A
63
−18.387
5.998
−30.831
1.00
15.67


ATOM
470
CA
ILE
A
63
−18.910
4.628
−30.810
1.00
15.86


ATOM
471
CB
ILE
A
63
−17.803
3.610
−30.372
1.00
15.88


ATOM
472
CG1
ILE
A
63
−16.756
3.466
−31.478
1.00
14.98


ATOM
473
CD1
ILE
A
63
−15.375
2.976
−30.966
1.00
15.62


ATOM
474
CG2
ILE
A
63
−18.398
2.251
−30.016
1.00
15.96


ATOM
475
C
ILE
A
63
−20.156
4.538
−29.920
1.00
16.39


ATOM
476
O
ILE
A
63
−21.137
3.854
−30.272
1.00
16.90


ATOM
477
N
ASP
A
64
−20.129
5.242
−28.796
1.00
16.51


ATOM
478
CA
ASP
A
64
−21.299
5.324
−27.922
1.00
17.76


ATOM
479
CB
ASP
A
64
−20.953
6.022
−26.594
1.00
17.81


ATOM
480
CG
ASP
A
64
−20.089
5.164
−25.682
1.00
17.84


ATOM
481
OD1
ASP
A
64
−19.883
3.944
−25.963
1.00
18.57


ATOM
482
OD2
ASP
A
64
−19.595
5.699
−24.659
1.00
20.95


ATOM
483
C
ASP
A
64
−22.492
5.982
−28.617
1.00
18.64


ATOM
484
O
ASP
A
64
−23.617
5.493
−28.507
1.00
20.85


ATOM
485
N
ARG
A
65
−22.262
7.070
−29.348
1.00
19.57


ATOM
486
CA
ARG
A
65
−23.341
7.750
−30.097
1.00
20.59


ATOM
487
CB
ARG
A
65
−22.823
9.046
−30.733
1.00
20.62


ATOM
488
CG
ARG
A
65
−22.465
10.083
−29.693
1.00
25.00


ATOM
489
CD
ARG
A
65
−22.010
11.385
−30.324
1.00
28.44


ATOM
490
NE
ARG
A
65
−23.106
12.071
−30.990
1.00
31.14


ATOM
491
CZ
ARG
A
65
−23.968
12.878
−30.373
1.00
32.75


ATOM
492
NH1
ARG
A
65
−23.873
13.095
−29.060
1.00
32.10


ATOM
493
NH2
ARG
A
65
−24.928
13.459
−31.080
1.00
32.31


ATOM
494
C
ARG
A
65
−23.907
6.841
−31.184
1.00
20.83


ATOM
495
O
ARG
A
65
−25.129
6.711
−31.357
1.00
20.48


ATOM
496
N
PHE
A
66
−22.998
6.213
−31.910
1.00
20.52


ATOM
497
CA
PHE
A
66
−23.340
5.271
−32.966
1.00
21.42


ATOM
498
CB
PHE
A
66
−22.046
4.778
−33.604
1.00
21.97


ATOM
499
CG
PHE
A
66
−22.224
3.603
−34.520
1.00
21.97


ATOM
500
CD1
PHE
A
66
−22.601
3.791
−35.844
1.00
23.23


ATOM
501
CE1
PHE
A
66
−22.768
2.690
−36.699
1.00
22.87


ATOM
502
CZ
PHE
A
66
−22.552
1.409
−36.221
1.00
22.60


ATOM
503
CE2
PHE
A
66
−22.165
1.216
−34.895
1.00
23.74


ATOM
504
CD2
PHE
A
66
−22.006
2.309
−34.054
1.00
23.07


ATOM
505
C
PHE
A
66
−24.152
4.084
−32.415
1.00
21.98


ATOM
506
O
PHE
A
66
−25.040
3.551
−33.099
1.00
21.80


ATOM
507
N
THR
A
67
−23.831
3.654
−31.195
1.00
22.48


ATOM
508
CA
THR
A
67
−24.546
2.537
−30.576
1.00
23.79


ATOM
509
CB
THR
A
67
−23.809
1.999
−29.333
1.00
23.68


ATOM
510
OG1
THR
A
67
−22.551
1.439
−29.745
1.00
23.93


ATOM
511
CG2
THR
A
67
−24.613
0.881
−28.653
1.00
23.90


ATOM
512
C
THR
A
67
−25.992
2.925
−30.258
1.00
24.77


ATOM
513
O
THR
A
67
−26.893
2.090
−30.349
1.00
25.31


ATOM
514
N
GLU
A
68
−26.207
4.189
−29.916
1.00
25.62


ATOM
515
CA
GLU
A
68
−27.540
4.688
−29.616
1.00
27.41


ATOM
516
CB
GLU
A
68
−27.466
6.038
−28.894
1.00
28.13


ATOM
517
CG
GLU
A
68
−26.997
5.951
−27.446
1.00
32.86


ATOM
518
CD
GLU
A
68
−28.095
5.487
−26.468
1.00
38.33


ATOM
519
OE1
GLU
A
68
−29.241
5.982
−26.542
1.00
40.42


ATOM
520
OE2
GLU
A
68
−27.799
4.633
−25.607
1.00
42.48


ATOM
521
C
GLU
A
68
−28.418
4.784
−30.873
1.00
27.86


ATOM
522
O
GLU
A
68
−29.602
4.429
−30.845
1.00
28.00


ATOM
523
N
THR
A
69
−27.833
5.260
−31.968
1.00
27.32


ATOM
524
CA
THR
A
69
−28.540
5.373
−33.241
1.00
27.32


ATOM
525
CB
THR
A
69
−29.113
6.792
−33.451
1.00
27.49


ATOM
526
OG1
THR
A
69
−29.922
7.153
−32.334
1.00
30.86


ATOM
527
CG2
THR
A
69
−29.945
6.843
−34.719
1.00
29.06


ATOM
528
C
THR
A
69
−27.563
5.133
−34.359
1.00
26.07


ATOM
529
O
THR
A
69
−26.619
5.905
−34.523
1.00
25.25


ATOM
530
N
TYR
A
70
−27.790
4.064
−35.123
1.00
25.65


ATOM
531
CA
TYR
A
70
−26.948
3.738
−36.267
1.00
25.36


ATOM
532
CB
TYR
A
70
−27.480
2.504
−37.013
1.00
25.26


ATOM
533
CG
TYR
A
70
−26.638
2.104
−38.217
1.00
25.62


ATOM
534
CD1
TYR
A
70
−26.949
2.567
−39.498
1.00
25.62


ATOM
535
CE1
TYR
A
70
−26.190
2.201
−40.611
1.00
26.48


ATOM
536
CZ
TYR
A
70
−25.099
1.360
−40.437
1.00
25.36


ATOM
537
OH
TYR
A
70
−24.354
0.995
−41.520
1.00
24.80


ATOM
538
CE2
TYR
A
70
−24.770
0.883
−39.175
1.00
25.59


ATOM
539
CD2
TYR
A
70
−25.538
1.259
−38.071
1.00
25.36


ATOM
540
C
TYR
A
70
−26.816
4.909
−37.230
1.00
25.38


ATOM
541
O
TYR
A
70
−27.802
5.573
−37.583
1.00
24.87


ATOM
542
N
ASP
A
71
−25.575
5.127
−37.666
1.00
25.16


ATOM
543
CA
ASP
A
71
−25.188
6.210
−38.550
1.00
25.40


ATOM
544
CB
ASP
A
71
−24.668
7.404
−37.724
1.00
25.60


ATOM
545
CG
ASP
A
71
−24.361
8.642
−38.573
1.00
26.17


ATOM
546
OD1
ASP
A
71
−23.801
8.526
−39.681
1.00
25.82


ATOM
547
OD2
ASP
A
71
−24.675
9.755
−38.108
1.00
27.85


ATOM
548
C
ASP
A
71
−24.061
5.619
−39.386
1.00
25.64


ATOM
549
O
ASP
A
71
−22.956
5.377
−38.875
1.00
24.82


ATOM
550
N
ALA
A
72
−24.347
5.379
−40.665
1.00
24.95


ATOM
551
CA
ALA
A
72
−23.380
4.764
−41.586
1.00
24.24


ATOM
552
CB
ALA
A
72
−24.047
4.434
−42.921
1.00
24.35


ATOM
553
C
ALA
A
72
−22.152
5.657
−41.812
1.00
24.04


ATOM
554
O
ALA
A
72
−21.054
5.159
−42.086
1.00
23.21


ATOM
555
N
GLY
A
73
−22.356
6.970
−41.695
1.00
23.63


ATOM
556
CA
GLY
A
73
−21.265
7.938
−41.761
1.00
24.20


ATOM
557
C
GLY
A
73
−20.285
7.809
−40.596
1.00
23.85


ATOM
558
O
GLY
A
73
−19.067
7.927
−40.806
1.00
24.81


ATOM
559
N
LEU
A
74
−20.798
7.588
−39.376
1.00
22.78


ATOM
560
CA
LEU
A
74
−19.927
7.347
−38.232
1.00
22.21


ATOM
561
CB
LEU
A
74
−20.662
7.449
−36.879
1.00
22.58


ATOM
562
CG
LEU
A
74
−21.132
8.846
−36.434
1.00
24.06


ATOM
563
CD1
LEU
A
74
−21.732
8.793
−35.019
1.00
22.31


ATOM
564
CD2
LEU
A
74
−20.002
9.869
−36.503
1.00
26.04


ATOM
565
C
LEU
A
74
−19.256
5.999
−38.370
1.00
21.94


ATOM
566
O
LEU
A
74
−18.060
5.872
−38.098
1.00
20.62


ATOM
567
N
GLN
A
75
−20.019
4.988
−38.814
1.00
21.47


ATOM
568
CA
GLN
A
75
−19.451
3.654
−38.989
1.00
21.07


ATOM
569
CB
GLN
A
75
−20.469
2.709
−39.619
1.00
21.44


ATOM
570
CG
GLN
A
75
−20.002
1.280
−39.594
1.00
23.11


ATOM
571
CD
GLN
A
75
−21.101
0.312
−39.945
1.00
24.74


ATOM
572
OE1
GLN
A
75
−21.273
−0.719
−39.290
1.00
26.57


ATOM
573
NE2
GLN
A
75
−21.872
0.654
−40.950
1.00
23.71


ATOM
574
C
GLN
A
75
−18.219
3.704
−39.889
1.00
21.05


ATOM
575
O
GLN
A
75
−17.229
3.046
−39.611
1.00
21.10


ATOM
576
N
ARG
A
76
−18.294
4.466
−40.975
1.00
20.73


ATOM
577
CA
ARG
A
76
−17.184
4.551
−41.910
1.00
21.56


ATOM
578
CB
ARG
A
76
−17.544
5.460
−43.101
1.00
22.06


ATOM
579
CG
ARG
A
76
−16.452
5.627
−44.168
1.00
24.00


ATOM
580
CD
ARG
A
76
−15.586
6.895
−43.934
1.00
27.60


ATOM
581
NE
ARG
A
76
−16.275
8.150
−44.280
1.00
30.93


ATOM
582
CZ
ARG
A
76
−15.778
9.378
−44.082
1.00
32.20


ATOM
583
NH1
ARG
A
76
−14.572
9.556
−43.535
1.00
30.72


ATOM
584
NH2
ARG
A
76
−16.491
10.443
−44.437
1.00
32.29


ATOM
585
C
ARG
A
76
−15.942
5.063
−41.162
1.00
20.85


ATOM
586
O
ARG
A
76
−14.858
4.514
−41.296
1.00
20.93


ATOM
587
N
ARG
A
77
−16.116
6.119
−40.378
1.00
20.17


ATOM
588
CA
ARG
A
77
−14.990
6.723
−39.631
1.00
19.61


ATOM
589
CB
ARG
A
77
−15.419
8.058
−39.009
1.00
19.11


ATOM
590
CG
ARG
A
77
−15.719
9.106
−40.075
1.00
20.18


ATOM
591
CD
ARG
A
77
−16.379
10.299
−39.459
1.00
22.42


ATOM
592
NE
ARG
A
77
−16.489
11.411
−40.396
1.00
24.06


ATOM
593
CZ
ARG
A
77
−17.501
11.592
−41.243
1.00
27.81


ATOM
594
NH1
ARG
A
77
−18.508
10.714
−41.303
1.00
28.01


ATOM
595
NH2
ARG
A
77
−17.509
12.658
−42.033
1.00
27.19


ATOM
596
C
ARG
A
77
−14.425
5.789
−38.570
1.00
18.77


ATOM
597
O
ARG
A
77
−13.197
5.685
−38.411
1.00
18.64


ATOM
598
N
ILE
A
78
−15.320
5.117
−37.852
1.00
17.79


ATOM
599
CA
ILE
A
78
−14.931
4.125
−36.857
1.00
18.13


ATOM
600
CB
ILE
A
78
−16.165
3.514
−36.151
1.00
17.88


ATOM
601
CG1
ILE
A
78
−16.862
4.564
−35.282
1.00
18.78


ATOM
602
CD1
ILE
A
78
−18.274
4.154
−34.879
1.00
19.16


ATOM
603
CG2
ILE
A
78
−15.772
2.279
−35.308
1.00
18.15


ATOM
604
C
ILE
A
78
−14.105
3.012
−37.491
1.00
18.09


ATOM
605
O
ILE
A
78
−13.088
2.612
−36.949
1.00
17.42


ATOM
606
N
GLU
A
79
−14.565
2.495
−38.631
1.00
18.82


ATOM
607
CA
GLU
A
79
−13.826
1.446
−39.341
1.00
20.22


ATOM
608
CB
GLU
A
79
−14.587
1.017
−40.609
1.00
20.36


ATOM
609
CG
GLU
A
79
−15.811
0.136
−40.312
1.00
22.29


ATOM
610
CD
GLU
A
79
−16.633
−0.206
−41.565
1.00
22.92


ATOM
611
OE1
GLU
A
79
−16.345
0.328
−42.670
1.00
26.99


ATOM
612
OE2
GLU
A
79
−17.579
−1.012
−41.425
1.00
25.69


ATOM
613
C
GLU
A
79
−12.418
1.918
−39.704
1.00
18.80


ATOM
614
O
GLU
A
79
−11.450
1.191
−39.507
1.00
19.44


ATOM
615
N
GLN
A
80
−12.301
3.147
−40.211
1.00
18.84


ATOM
616
CA
GLN
A
80
−10.998
3.661
−40.636
1.00
17.90


ATOM
617
CB
GLN
A
80
−11.149
4.921
−41.482
1.00
18.92


ATOM
618
CG
GLN
A
80
−11.794
4.593
−42.844
1.00
21.99


ATOM
619
CD
GLN
A
80
−12.040
5.799
−43.707
1.00
27.48


ATOM
620
OE1
GLN
A
80
−12.265
6.898
−43.223
1.00
30.45


ATOM
621
NE2
GLN
A
80
−12.037
5.586
−45.013
1.00
32.63


ATOM
622
C
GLN
A
80
−10.059
3.892
−39.456
1.00
17.64


ATOM
623
O
GLN
A
80
−8.862
3.612
−39.535
1.00
17.21


ATOM
624
N
TYR
A
81
−10.607
4.408
−38.365
1.00
17.29


ATOM
625
CA
TYR
A
81
−9.839
4.552
−37.122
1.00
17.64


ATOM
626
CB
TYR
A
81
−10.750
5.139
−36.023
1.00
17.24


ATOM
627
CG
TYR
A
81
−10.188
4.973
−34.621
1.00
17.79


ATOM
628
CD1
TYR
A
81
−9.085
5.728
−34.184
1.00
16.36


ATOM
629
CE1
TYR
A
81
−8.561
5.568
−32.882
1.00
17.45


ATOM
630
CZ
TYR
A
81
−9.146
4.646
−32.009
1.00
16.35


ATOM
631
OH
TYR
A
81
−8.654
4.457
−30.724
1.00
17.06


ATOM
632
CE2
TYR
A
81
−10.238
3.890
−32.423
1.00
17.51


ATOM
633
CD2
TYR
A
81
−10.754
4.055
−33.729
1.00
17.00


ATOM
634
C
TYR
A
81
−9.271
3.197
−36.686
1.00
18.04


ATOM
635
O
TYR
A
81
−8.098
3.083
−36.321
1.00
17.85


ATOM
636
N
ILE
A
82
−10.096
2.159
−36.746
1.00
17.99


ATOM
637
CA
ILE
A
82
−9.661
0.839
−36.295
1.00
19.35


ATOM
638
CB
ILE
A
82
−10.844
−0.166
−36.187
1.00
18.93


ATOM
639
CG1
ILE
A
82
−11.753
0.233
−35.017
1.00
19.40


ATOM
640
CD1
ILE
A
82
−13.093
−0.565
−34.896
1.00
20.46


ATOM
641
CG2
ILE
A
82
−10.301
−1.587
−35.984
1.00
20.61


ATOM
642
C
ILE
A
82
−8.547
0.292
−37.194
1.00
19.92


ATOM
643
O
ILE
A
82
−7.543
−0.239
−36.708
1.00
20.26


ATOM
644
N
THR
A
83
−8.713
0.432
−38.503
1.00
20.05


ATOM
645
CA
THR
A
83
−7.709
−0.100
−39.406
1.00
21.11


ATOM
646
CB
THR
A
83
−8.241
−0.297
−40.845
1.00
21.63


ATOM
647
OG1
THR
A
83
−8.830
0.902
−41.306
1.00
25.88


ATOM
648
CG2
THR
A
83
−9.330
−1.347
−40.851
1.00
21.56


ATOM
649
C
THR
A
83
−6.410
0.690
−39.337
1.00
20.59


ATOM
650
O
THR
A
83
−5.338
0.105
−39.511
1.00
20.72


ATOM
651
N
ALA
A
84
−6.494
1.997
−39.050
1.00
19.51


ATOM
652
CA
ALA
A
84
−5.292
2.809
−38.777
1.00
19.37


ATOM
653
CB
ALA
A
84
−5.652
4.290
−38.507
1.00
19.42


ATOM
654
C
ALA
A
84
−4.436
2.231
−37.643
1.00
19.32


ATOM
655
O
ALA
A
84
−3.208
2.370
−37.649
1.00
19.47


ATOM
656
N
GLN
A
85
−5.063
1.535
−36.695
1.00
19.34


ATOM
657
CA
GLN
A
85
−4.325
0.998
−35.544
1.00
18.78


ATOM
658
CB
GLN
A
85
−5.266
0.609
−34.396
1.00
19.29


ATOM
659
CG
GLN
A
85
−6.260
1.735
−34.007
1.00
17.98


ATOM
660
CD
GLN
A
85
−5.593
3.098
−33.830
1.00
17.20


ATOM
661
OE1
GLN
A
85
−6.021
4.095
−34.418
1.00
20.82


ATOM
662
NE2
GLN
A
85
−4.540
3.143
−33.034
1.00
13.47


ATOM
663
C
GLN
A
85
−3.447
−0.178
−35.932
1.00
19.08


ATOM
664
O
GLN
A
85
−2.478
−0.473
−35.251
1.00
17.94


ATOM
665
N
VAL
A
86
−3.808
−0.838
−37.032
1.00
19.32


ATOM
666
CA
VAL
A
86
−2.999
−1.928
−37.588
1.00
20.79


ATOM
667
CB
VAL
A
86
−3.670
−2.581
−38.823
1.00
21.18


ATOM
668
CG1
VAL
A
86
−2.712
−3.615
−39.454
1.00
22.66


ATOM
669
CG2
VAL
A
86
−4.980
−3.250
−38.400
1.00
21.47


ATOM
670
C
VAL
A
86
−1.617
−1.381
−37.940
1.00
20.63


ATOM
671
O
VAL
A
86
−0.602
−1.930
−37.520
1.00
21.41


ATOM
672
N
THR
A
87
−1.604
−0.251
−38.641
1.00
20.67


ATOM
673
CA
THR
A
87
−0.361
0.419
−39.015
1.00
21.10


ATOM
674
CB
THR
A
87
−0.659
1.583
−39.986
1.00
21.59


ATOM
675
OG1
THR
A
87
−1.176
1.033
−41.202
1.00
23.63


ATOM
676
CG2
THR
A
87
0.585
2.370
−40.305
1.00
22.02


ATOM
677
C
THR
A
87
0.412
0.881
−37.795
1.00
20.28


ATOM
678
O
THR
A
87
1.620
0.641
−37.679
1.00
20.22


ATOM
679
N
LEU
A
88
−0.280
1.543
−36.874
1.00
19.11


ATOM
680
CA
LEU
A
88
0.367
2.097
−35.697
1.00
18.71


ATOM
681
CB
LEU
A
88
−0.585
3.015
−34.903
1.00
18.08


ATOM
682
CG
LEU
A
88
−1.016
4.294
−35.596
1.00
18.50


ATOM
683
CD1
LEU
A
88
−2.038
5.058
−34.706
1.00
19.12


ATOM
684
CD2
LEU
A
88
0.206
5.193
−35.937
1.00
19.81


ATOM
685
C
LEU
A
88
0.976
1.057
−34.780
1.00
18.33


ATOM
686
O
LEU
A
88
2.101
1.244
−34.336
1.00
18.76


ATOM
687
N
GLN
A
89
0.255
−0.029
−34.492
1.00
18.75


ATOM
688
CA
GLN
A
89
0.809
−1.085
−33.623
1.00
19.67


ATOM
689
CB
GLN
A
89
−0.199
−2.201
−33.373
1.00
19.69


ATOM
690
CG
GLN
A
89
−1.397
−1.775
−32.564
1.00
19.25


ATOM
691
CD
GLN
A
89
−2.140
−2.951
−32.004
1.00
20.83


ATOM
692
OE1
GLN
A
89
−2.121
−4.037
−32.580
1.00
19.26


ATOM
693
NE2
GLN
A
89
−2.802
−2.751
−30.861
1.00
19.61


ATOM
694
C
GLN
A
89
2.097
−1.683
−34.203
1.00
20.68


ATOM
695
O
GLN
A
89
3.013
−2.026
−33.461
1.00
21.21


ATOM
696
N
GLY
A
90
2.164
−1.778
−35.524
1.00
21.75


ATOM
697
CA
GLY
A
90
3.330
−2.374
−36.173
1.00
23.35


ATOM
698
C
GLY
A
90
4.604
−1.552
−36.096
1.00
24.79


ATOM
699
O
GLY
A
90
5.699
−2.104
−36.299
1.00
25.52


ATOM
700
N
ASN
A
91
4.477
−0.252
−35.796
1.00
25.17


ATOM
701
CA
ASN
A
91
5.596
0.714
−35.870
1.00
26.14


ATOM
702
CB
ASN
A
91
5.108
2.161
−35.653
1.00
26.83


ATOM
703
CG
ASN
A
91
4.615
2.849
−36.919
1.00
29.67


ATOM
704
OD1
ASN
A
91
4.869
2.414
−38.051
1.00
34.53


ATOM
705
ND2
ASN
A
91
3.927
3.981
−36.724
1.00
32.76


ATOM
706
C
ASN
A
91
6.656
0.489
−34.820
1.00
25.67


ATOM
707
O
ASN
A
91
6.346
0.385
−33.644
1.00
25.70


ATOM
708
N
SER
A
92
7.918
0.472
−35.227
1.00
25.32


ATOM
709
CA
SER
A
92
8.990
0.668
−34.257
1.00
25.27


ATOM
710
CB
SER
A
92
10.314
0.107
−34.775
1.00
26.03


ATOM
711
OG
SER
A
92
10.212
−1.305
−34.803
1.00
30.67


ATOM
712
C
SER
A
92
9.103
2.171
−34.003
1.00
23.80


ATOM
713
O
SER
A
92
9.055
2.979
−34.942
1.00
24.56


ATOM
714
N
ASN
A
93
9.246
2.544
−32.743
1.00
22.30


ATOM
715
CA
ASN
A
93
9.236
3.953
−32.383
1.00
21.23


ATOM
716
CB
ASN
A
93
7.769
4.423
−32.201
1.00
21.41


ATOM
717
CG
ASN
A
93
7.075
3.704
−31.051
1.00
19.23


ATOM
718
OD1
ASN
A
93
7.564
3.736
−29.927
1.00
17.81


ATOM
719
ND2
ASN
A
93
5.974
3.024
−31.335
1.00
19.43


ATOM
720
C
ASN
A
93
10.103
4.149
−31.150
1.00
20.39


ATOM
721
O
ASN
A
93
10.625
3.154
−30.607
1.00
19.46


ATOM
722
N
PRO
A
94
10.337
5.410
−30.732
1.00
19.95


ATOM
723
CA
PRO
A
94
11.228
5.604
−29.574
1.00
19.89


ATOM
724
CB
PRO
A
94
11.235
7.131
−29.385
1.00
19.51


ATOM
725
CG
PRO
A
94
10.988
7.653
−30.753
1.00
19.96


ATOM
726
CD
PRO
A
94
9.952
6.717
−31.325
1.00
19.99


ATOM
727
C
PRO
A
94
10.870
4.898
−28.263
1.00
20.64


ATOM
728
O
PRO
A
94
11.756
4.727
−27.430
1.00
20.54


ATOM
729
N
SER
A
95
9.610
4.485
−28.073
1.00
20.36


ATOM
730
CA
SER
A
95
9.264
3.674
−26.902
1.00
21.00


ATOM
731
CB
SER
A
95
7.770
3.736
−26.587
1.00
20.05


ATOM
732
OG
SER
A
95
7.413
5.036
−26.147
1.00
19.97


ATOM
733
C
SER
A
95
9.679
2.204
−27.066
1.00
21.73


ATOM
734
O
SER
A
95
9.809
1.499
−26.072
1.00
22.10


ATOM
735
N
GLY
A
96
9.853
1.748
−28.306
1.00
21.90


ATOM
736
CA
GLY
A
96
10.229
0.350
−28.562
1.00
23.56


ATOM
737
C
GLY
A
96
9.506
−0.196
−29.774
1.00
24.14


ATOM
738
O
GLY
A
96
9.121
0.557
−30.664
1.00
24.24


ATOM
739
N
SER
A
97
9.315
−1.510
−29.828
1.00
25.24


ATOM
740
CA
SER
A
97
8.703
−2.116
−31.000
1.00
25.77


ATOM
741
CB
SER
A
97
9.751
−2.874
−31.834
1.00
27.15


ATOM
742
OG
SER
A
97
10.120
−4.086
−31.189
1.00
30.57


ATOM
743
C
SER
A
97
7.590
−3.042
−30.571
1.00
25.27


ATOM
744
O
SER
A
97
7.346
−3.199
−29.376
1.00
24.85


ATOM
745
N
LEU
A
98
6.930
−3.655
−31.543
1.00
24.82


ATOM
746
CA
LEU
A
98
5.826
−4.559
−31.252
1.00
25.88


ATOM
747
CB
LEU
A
98
4.982
−4.813
−32.504
1.00
25.31


ATOM
748
CG
LEU
A
98
3.714
−5.673
−32.420
1.00
25.89


ATOM
749
CD1
LEU
A
98
2.745
−5.169
−31.337
1.00
25.58


ATOM
750
CD2
LEU
A
98
3.006
−5.724
−33.778
1.00
26.27


ATOM
751
C
LEU
A
98
6.310
−5.866
−30.604
1.00
26.75


ATOM
752
O
LEU
A
98
5.607
−6.438
−29.762
1.00
27.41


ATOM
753
N
ALA
A
99
7.528
−6.290
−30.950
1.00
27.23


ATOM
754
CA
ALA
A
99
8.074
−7.590
−30.533
1.00
27.89


ATOM
755
CB
ALA
A
99
9.566
−7.700
−30.935
1.00
27.68


ATOM
756
C
ALA
A
99
7.893
−7.911
−29.053
1.00
27.86


ATOM
757
O
ALA
A
99
7.450
−9.007
−28.711
1.00
28.77


ATOM
758
N
ASP
A
100
8.241
−6.966
−28.181
1.00
27.75


ATOM
759
CA
ASP
A
100
8.030
−7.137
−26.741
1.00
27.20


ATOM
760
CB
ASP
A
100
9.328
−6.937
−25.966
1.00
27.10


ATOM
761
CG
ASP
A
100
9.845
−5.525
−26.038
1.00
30.19


ATOM
762
OD1
ASP
A
100
10.891
−5.281
−25.419
1.00
32.28


ATOM
763
OD2
ASP
A
100
9.225
−4.654
−26.694
1.00
30.36


ATOM
764
C
ASP
A
100
6.905
−6.256
−26.173
1.00
25.74


ATOM
765
O
ASP
A
100
6.761
−6.108
−24.956
1.00
26.33


ATOM
766
N
GLY
A
101
6.118
−5.683
−27.075
1.00
24.93


ATOM
767
CA
GLY
A
101
4.982
−4.853
−26.707
1.00
23.22


ATOM
768
C
GLY
A
101
5.326
−3.418
−26.342
1.00
22.68


ATOM
769
O
GLY
A
101
4.419
−2.580
−26.287
1.00
21.48


ATOM
770
N
SER
A
102
6.609
−3.117
−26.126
1.00
21.53


ATOM
771
CA
SER
A
102
6.996
−1.815
−25.563
1.00
21.49


ATOM
772
CB
SER
A
102
8.483
−1.739
−25.199
1.00
22.07


ATOM
773
OG
SER
A
102
9.283
−1.958
−26.345
1.00
21.77


ATOM
774
C
SER
A
102
6.604
−0.643
−26.449
1.00
20.66


ATOM
775
O
SER
A
102
6.279
0.403
−25.925
1.00
20.67


ATOM
776
N
GLY
A
103
6.636
−0.819
−27.771
1.00
19.97


ATOM
777
CA
GLY
A
103
6.257
0.255
−28.707
1.00
19.46


ATOM
778
C
GLY
A
103
4.824
0.777
−28.539
1.00
18.94


ATOM
779
O
GLY
A
103
4.525
1.903
−28.945
1.00
18.13


ATOM
780
N
LEU
A
104
3.939
−0.043
−27.956
1.00
18.13


ATOM
781
CA
LEU
A
104
2.517
0.326
−27.818
1.00
16.92


ATOM
782
CB
LEU
A
104
1.672
−0.924
−27.447
1.00
17.28


ATOM
783
CG
LEU
A
104
1.715
−2.104
−28.430
1.00
16.76


ATOM
784
CD1
LEU
A
104
1.072
−3.356
−27.836
1.00
21.62


ATOM
785
CD2
LEU
A
104
1.069
−1.751
−29.761
1.00
19.06


ATOM
786
C
LEU
A
104
2.283
1.464
−26.798
1.00
16.57


ATOM
787
O
LEU
A
104
1.202
2.092
−26.807
1.00
16.79


ATOM
788
N
GLY
A
105
3.279
1.713
−25.936
1.00
14.86


ATOM
789
CA
GLY
A
105
3.255
2.802
−24.938
1.00
15.94


ATOM
790
C
GLY
A
105
3.558
4.199
−25.482
1.00
15.05


ATOM
791
O
GLY
A
105
3.481
5.179
−24.755
1.00
15.93


ATOM
792
N
GLU
A
106
3.869
4.292
−26.780
1.00
14.63


ATOM
793
CA
GLU
A
106
4.236
5.548
−27.416
1.00
14.49


ATOM
794
CB
GLU
A
106
4.728
5.250
−28.847
1.00
13.83


ATOM
795
CG
GLU
A
106
5.215
6.470
−29.678
1.00
16.14


ATOM
796
CD
GLU
A
106
6.479
7.155
−29.139
1.00
18.44


ATOM
797
OE1
GLU
A
106
6.978
6.817
−28.044
1.00
21.44


ATOM
798
OE2
GLU
A
106
6.972
8.083
−29.817
1.00
21.04


ATOM
799
C
GLU
A
106
3.012
6.484
−27.413
1.00
14.32


ATOM
800
O
GLU
A
106
1.928
6.074
−27.828
1.00
15.51


ATOM
801
N
PRO
A
107
3.164
7.706
−26.890
1.00
15.21


ATOM
802
CA
PRO
A
107
2.025
8.645
−26.772
1.00
14.90


ATOM
803
CB
PRO
A
107
2.598
9.809
−25.951
1.00
15.27


ATOM
804
CG
PRO
A
107
3.833
9.290
−25.310
1.00
17.25


ATOM
805
CD
PRO
A
107
4.385
8.238
−26.243
1.00
14.35


ATOM
806
C
PRO
A
107
1.468
9.219
−28.066
1.00
14.48


ATOM
807
O
PRO
A
107
0.263
9.371
−28.172
1.00
13.94


ATOM
808
N
LYS
A
108
2.320
9.567
−29.027
1.00
14.24


ATOM
809
CA
LYS
A
108
1.837
10.309
−30.204
1.00
14.51


ATOM
810
CB
LYS
A
108
1.853
11.828
−29.960
1.00
14.26


ATOM
811
CG
LYS
A
108
3.225
12.524
−30.029
1.00
13.88


ATOM
812
CD
LYS
A
108
3.102
14.033
−29.775
1.00
15.15


ATOM
813
CE
LYS
A
108
4.299
14.820
−30.283
1.00
17.08


ATOM
814
NZ
LYS
A
108
4.341
16.279
−29.836
1.00
15.57


ATOM
815
C
LYS
A
108
2.608
9.953
−31.454
1.00
14.69


ATOM
816
O
LYS
A
108
3.734
9.442
−31.377
1.00
14.67


ATOM
817
N
PHE
A
109
1.976
10.203
−32.594
1.00
14.54


ATOM
818
CA
PHE
A
109
2.530
9.860
−33.902
1.00
15.20


ATOM
819
CB
PHE
A
109
1.839
8.594
−34.451
1.00
15.67


ATOM
820
CG
PHE
A
109
1.973
7.407
−33.553
1.00
16.81


ATOM
821
CD1
PHE
A
109
1.081
7.219
−32.490
1.00
17.93


ATOM
822
CE1
PHE
A
109
1.205
6.117
−31.649
1.00
21.93


ATOM
823
CZ
PHE
A
109
2.241
5.210
−31.834
1.00
19.06


ATOM
824
CE2
PHE
A
109
3.141
5.384
−32.883
1.00
20.27


ATOM
825
CD2
PHE
A
109
3.003
6.492
−33.737
1.00
19.44


ATOM
826
C
PHE
A
109
2.301
10.992
−34.881
1.00
15.19


ATOM
827
O
PHE
A
109
1.450
11.861
−34.655
1.00
14.41


ATOM
828
N
GLU
A
110
3.039
10.971
−35.993
1.00
15.14


ATOM
829
CA
GLU
A
110
2.756
11.905
−37.077
1.00
15.24


ATOM
830
CB
GLU
A
110
3.905
11.933
−38.103
1.00
15.00


ATOM
831
CG
GLU
A
110
5.302
12.204
−37.493
1.00
16.29


ATOM
832
CD
GLU
A
110
5.554
13.673
−37.174
1.00
17.52


ATOM
833
OE1
GLU
A
110
4.708
14.544
−37.504
1.00
17.65


ATOM
834
OE2
GLU
A
110
6.619
13.963
−36.587
1.00
18.74


ATOM
835
C
GLU
A
110
1.462
11.476
−37.762
1.00
15.32


ATOM
836
O
GLU
A
110
1.093
10.278
−37.753
1.00
15.26


ATOM
837
N
LEU
A
111
0.776
12.445
−38.360
1.00
15.59


ATOM
838
CA
LEU
A
111
−0.522
12.186
−39.009
1.00
16.33


ATOM
839
CB
LEU
A
111
−1.265
13.510
−39.163
1.00
16.57


ATOM
840
CG
LEU
A
111
−1.770
13.908
−37.756
1.00
18.11


ATOM
841
CD1
LEU
A
111
−1.819
15.405
−37.574
1.00
21.43


ATOM
842
CD2
LEU
A
111
−3.151
13.204
−37.506
1.00
18.74


ATOM
843
C
LEU
A
111
−0.409
11.436
−40.350
1.00
17.37


ATOM
844
O
LEU
A
111
−1.426
11.023
−40.944
1.00
17.23


ATOM
845
N
THR
A
112
0.833
11.258
−40.815
1.00
17.42


ATOM
846
CA
THR
A
112
1.144
10.301
−41.887
1.00
17.92


ATOM
847
CB
THR
A
112
2.512
10.619
−42.499
1.00
17.98


ATOM
848
OG1
THR
A
112
3.476
10.702
−41.445
1.00
18.32


ATOM
849
CG2
THR
A
112
2.486
11.945
−43.228
1.00
19.47


ATOM
850
C
THR
A
112
1.215
8.846
−41.356
1.00
18.90


ATOM
851
O
THR
A
112
1.535
7.917
−42.117
1.00
17.68


ATOM
852
N
LEU
A
113
0.944
8.664
−40.055
1.00
18.48


ATOM
853
CA
LEU
A
113
1.041
7.379
−39.348
1.00
19.78


ATOM
854
CB
LEU
A
113
0.061
6.319
−39.904
1.00
19.45


ATOM
855
CG
LEU
A
113
−1.411
6.699
−40.074
1.00
21.71


ATOM
856
CD1
LEU
A
113
−2.194
5.470
−40.477
1.00
23.46


ATOM
857
CD2
LEU
A
113
−2.005
7.323
−38.800
1.00
21.59


ATOM
858
C
LEU
A
113
2.481
6.866
−39.338
1.00
20.43


ATOM
859
O
LEU
A
113
2.737
5.704
−39.653
1.00
20.96


ATOM
860
N
LYS
A
114
3.406
7.769
−39.024
1.00
20.07


ATOM
861
CA
LYS
A
114
4.826
7.460
−38.863
1.00
20.25


ATOM
862
CB
LYS
A
114
5.662
8.209
−39.899
1.00
20.95


ATOM
863
CG
LYS
A
114
5.432
7.725
−41.314
1.00
25.23


ATOM
864
CD
LYS
A
114
6.636
8.059
−42.184
1.00
33.76


ATOM
865
CE
LYS
A
114
6.551
7.360
−43.537
1.00
38.31


ATOM
866
NZ
LYS
A
114
5.285
7.711
−44.251
1.00
41.29


ATOM
867
C
LYS
A
114
5.252
7.874
−37.471
1.00
19.64


ATOM
868
O
LYS
A
114
4.576
8.708
−36.845
1.00
19.39


ATOM
869
N
PRO
A
115
6.376
7.318
−36.973
1.00
19.06


ATOM
870
CA
PRO
A
115
6.750
7.626
−35.601
1.00
18.72


ATOM
871
CB
PRO
A
115
7.963
6.712
−35.326
1.00
19.82


ATOM
872
CG
PRO
A
115
8.101
5.814
−36.500
1.00
20.93


ATOM
873
CD
PRO
A
115
7.339
6.412
−37.639
1.00
19.08


ATOM
874
C
PRO
A
115
7.156
9.093
−35.434
1.00
18.70


ATOM
875
O
PRO
A
115
7.694
9.724
−36.375
1.00
17.25


ATOM
876
N
PHE
A
116
6.844
9.628
−34.256
1.00
18.55


ATOM
877
CA
PHE
A
116
7.342
10.918
−33.805
1.00
18.27


ATOM
878
CB
PHE
A
116
6.359
11.566
−32.809
1.00
18.41


ATOM
879
CG
PHE
A
116
6.908
12.804
−32.151
1.00
17.33


ATOM
880
CD1
PHE
A
116
6.942
14.014
−32.847
1.00
16.98


ATOM
881
CE1
PHE
A
116
7.457
15.173
−32.254
1.00
16.22


ATOM
882
CZ
PHE
A
116
7.950
15.138
−30.935
1.00
15.88


ATOM
883
CE2
PHE
A
116
7.902
13.917
−30.216
1.00
16.74


ATOM
884
CD2
PHE
A
116
7.380
12.767
−30.825
1.00
16.05


ATOM
885
C
PHE
A
116
8.701
10.695
−33.141
1.00
19.14


ATOM
886
O
PHE
A
116
8.808
9.987
−32.134
1.00
19.66


ATOM
887
N
THR
A
117
9.746
11.299
−33.713
1.00
19.53


ATOM
888
CA
THR
A
117
11.116
11.020
−33.315
1.00
20.15


ATOM
889
CB
THR
A
117
12.042
10.999
−34.567
1.00
20.83


ATOM
890
OG1
THR
A
117
11.988
12.277
−35.222
1.00
22.59


ATOM
891
CG2
THR
A
117
11.576
9.895
−35.557
1.00
21.40


ATOM
892
C
THR
A
117
11.685
11.969
−32.263
1.00
20.34


ATOM
893
O
THR
A
117
12.813
11.768
−31.774
1.00
21.13


ATOM
894
N
GLY
A
118
10.943
13.017
−31.914
1.00
19.25


ATOM
895
CA
GLY
A
118
11.451
14.018
−30.974
1.00
19.41


ATOM
896
C
GLY
A
118
11.431
13.498
−29.541
1.00
19.75


ATOM
897
O
GLY
A
118
10.913
12.397
−29.281
1.00
19.99


ATOM
898
N
ASN
A
119
11.998
14.279
−28.622
1.00
19.56


ATOM
899
CA
ASN
A
119
11.958
13.954
−27.198
1.00
20.41


ATOM
900
CB
ASN
A
119
12.961
14.801
−26.419
1.00
21.50


ATOM
901
CG
ASN
A
119
14.377
14.612
−26.930
1.00
25.14


ATOM
902
OD1
ASN
A
119
14.779
13.500
−27.294
1.00
30.77


ATOM
903
ND2
ASN
A
119
15.131
15.693
−26.987
1.00
31.23


ATOM
904
C
ASN
A
119
10.550
14.194
−26.696
1.00
20.06


ATOM
905
O
ASN
A
119
9.881
15.089
−27.167
1.00
19.00


ATOM
906
N
TRP
A
120
10.084
13.348
−25.787
1.00
19.74


ATOM
907
CA
TRP
A
120
8.707
13.466
−25.316
1.00
19.03


ATOM
908
CB
TRP
A
120
7.717
12.917
−26.359
1.00
18.71


ATOM
909
CG
TRP
A
120
6.351
13.522
−26.162
1.00
19.76


ATOM
910
CD1
TRP
A
120
5.239
12.901
−25.673
1.00
19.81


ATOM
911
NE1
TRP
A
120
4.186
13.799
−25.593
1.00
19.49


ATOM
912
CE2
TRP
A
120
4.612
15.021
−26.042
1.00
19.44


ATOM
913
CD2
TRP
A
120
5.975
14.886
−26.410
1.00
19.35


ATOM
914
CE3
TRP
A
120
6.657
16.014
−26.895
1.00
18.94


ATOM
915
CZ3
TRP
A
120
5.959
17.220
−27.010
1.00
20.22


ATOM
916
CH2
TRP
A
120
4.602
17.315
−26.628
1.00
20.15


ATOM
917
CZ2
TRP
A
120
3.918
16.233
−26.160
1.00
18.83


ATOM
918
C
TRP
A
120
8.602
12.685
−24.001
1.00
18.80


ATOM
919
O
TRP
A
120
9.454
11.833
−23.722
1.00
18.83


ATOM
920
N
GLY
A
121
7.593
12.990
−23.189
1.00
17.91


ATOM
921
CA
GLY
A
121
7.314
12.189
−21.988
1.00
17.64


ATOM
922
C
GLY
A
121
6.721
10.834
−22.362
1.00
18.93


ATOM
923
O
GLY
A
121
5.499
10.704
−22.487
1.00
18.99


ATOM
924
N
ARG
A
122
7.589
9.828
−22.536
1.00
17.95


ATOM
925
CA
ARG
A
122
7.195
8.483
−22.958
1.00
17.86


ATOM
926
CB
ARG
A
122
7.686
8.193
−24.394
1.00
17.37


ATOM
927
CG
ARG
A
122
9.181
8.529
−24.626
1.00
19.53


ATOM
928
CD
ARG
A
122
9.689
7.987
−25.969
1.00
17.88


ATOM
929
NE
ARG
A
122
9.012
8.549
−27.159
1.00
18.08


ATOM
930
CZ
ARG
A
122
9.425
9.645
−27.807
1.00
18.06


ATOM
931
NH1
ARG
A
122
10.477
10.326
−27.366
1.00
16.88


ATOM
932
NH2
ARG
A
122
8.784
10.074
−28.892
1.00
17.35


ATOM
933
C
ARG
A
122
7.799
7.450
−21.976
1.00
17.48


ATOM
934
O
ARG
A
122
8.848
7.697
−21.396
1.00
17.69


ATOM
935
N
PRO
A
123
7.142
6.298
−21.781
1.00
17.14


ATOM
936
CA
PRO
A
123
5.886
5.916
−22.382
1.00
16.01


ATOM
937
CB
PRO
A
123
5.908
4.385
−22.266
1.00
16.51


ATOM
938
CG
PRO
A
123
6.585
4.144
−20.969
1.00
16.30


ATOM
939
CD
PRO
A
123
7.658
5.238
−20.873
1.00
16.95


ATOM
940
C
PRO
A
123
4.716
6.494
−21.581
1.00
15.29


ATOM
941
O
PRO
A
123
4.926
7.057
−20.521
1.00
15.07


ATOM
942
N
GLN
A
124
3.504
6.362
−22.120
1.00
14.60


ATOM
943
CA
GLN
A
124
2.289
6.675
−21.386
1.00
14.40


ATOM
944
CB
GLN
A
124
1.602
7.889
−22.001
1.00
14.71


ATOM
945
CG
GLN
A
124
2.442
9.186
−21.711
1.00
11.93


ATOM
946
CD
GLN
A
124
1.993
10.407
−22.472
1.00
15.67


ATOM
947
OE1
GLN
A
124
2.807
11.310
−22.758
1.00
15.75


ATOM
948
NE2
GLN
A
124
0.718
10.450
−22.822
1.00
9.85


ATOM
949
C
GLN
A
124
1.441
5.421
−21.511
1.00
14.28


ATOM
950
O
GLN
A
124
0.988
5.095
−22.604
1.00
14.63


ATOM
951
N
ARG
A
125
1.241
4.731
−20.390
1.00
13.50


ATOM
952
CA
ARG
A
125
0.700
3.382
−20.398
1.00
13.96


ATOM
953
CB
ARG
A
125
1.331
2.567
−19.256
1.00
14.28


ATOM
954
CG
ARG
A
125
2.864
2.703
−19.249
1.00
15.16


ATOM
955
CD
ARG
A
125
3.503
1.577
−18.439
1.00
18.07


ATOM
956
NE
ARG
A
125
4.924
1.827
−18.132
1.00
17.46


ATOM
957
CZ
ARG
A
125
5.944
1.334
−18.833
1.00
19.43


ATOM
958
NH1
ARG
A
125
5.728
0.601
−19.925
1.00
20.02


ATOM
959
NH2
ARG
A
125
7.197
1.596
−18.453
1.00
18.94


ATOM
960
C
ARG
A
125
−0.829
3.335
−20.359
1.00
14.48


ATOM
961
O
ARG
A
125
−1.424
2.262
−20.343
1.00
14.40


ATOM
962
N
ASP
A
126
−1.462
4.509
−20.374
1.00
14.03


ATOM
963
CA
ASP
A
126
−2.919
4.568
−20.542
1.00
13.39


ATOM
964
CB
ASP
A
126
−3.488
5.922
−20.067
1.00
13.17


ATOM
965
CG
ASP
A
126
−2.926
7.092
−20.845
1.00
14.17


ATOM
966
OD1
ASP
A
126
−1.713
7.108
−21.143
1.00
12.29


ATOM
967
OD2
ASP
A
126
−3.705
8.003
−21.187
1.00
16.69


ATOM
968
C
ASP
A
126
−3.350
4.306
−21.974
1.00
13.60


ATOM
969
O
ASP
A
126
−4.452
3.806
−22.189
1.00
13.56


ATOM
970
N
GLY
A
127
−2.491
4.634
−22.948
1.00
12.78


ATOM
971
CA
GLY
A
127
−2.886
4.558
−24.358
1.00
13.20


ATOM
972
C
GLY
A
127
−3.473
3.213
−24.794
1.00
12.77


ATOM
973
O
GLY
A
127
−4.579
3.150
−25.327
1.00
12.56


ATOM
974
N
PRO
A
128
−2.720
2.120
−24.613
1.00
13.94


ATOM
975
CA
PRO
A
128
−3.262
0.810
−24.978
1.00
12.97


ATOM
976
CB
PRO
A
128
−2.135
−0.162
−24.552
1.00
14.15


ATOM
977
CG
PRO
A
128
−0.907
0.656
−24.721
1.00
14.39


ATOM
978
CD
PRO
A
128
−1.323
2.010
−24.150
1.00
13.67


ATOM
979
C
PRO
A
128
−4.571
0.455
−24.255
1.00
13.30


ATOM
980
O
PRO
A
128
−5.433
−0.161
−24.869
1.00
13.72


ATOM
981
N
ALA
A
129
−4.718
0.852
−22.985
1.00
12.95


ATOM
982
CA
ALA
A
129
−5.963
0.611
−22.258
1.00
13.49


ATOM
983
CB
ALA
A
129
−5.806
1.016
−20.808
1.00
13.00


ATOM
984
C
ALA
A
129
−7.162
1.329
−22.923
1.00
13.43


ATOM
985
O
ALA
A
129
−8.217
0.721
−23.159
1.00
13.26


ATOM
986
N
LEU
A
130
−6.998
2.619
−23.221
1.00
12.51


ATOM
987
CA
LEU
A
130
−8.068
3.409
−23.813
1.00
12.69


ATOM
988
CB
LEU
A
130
−7.678
4.903
−23.806
1.00
12.56


ATOM
989
CG
LEU
A
130
−7.458
5.555
−22.426
1.00
14.76


ATOM
990
CD1
LEU
A
130
−6.959
6.991
−22.643
1.00
15.23


ATOM
991
CD2
LEU
A
130
−8.776
5.544
−21.651
1.00
15.30


ATOM
992
C
LEU
A
130
−8.410
2.934
−25.228
1.00
12.78


ATOM
993
O
LEU
A
130
−9.571
2.863
−25.607
1.00
12.83


ATOM
994
N
ARG
A
131
−7.386
2.601
−26.015
1.00
13.70


ATOM
995
CA
ARG
A
131
−7.630
2.070
−27.351
1.00
14.52


ATOM
996
CB
ARG
A
131
−6.316
1.925
−28.135
1.00
14.04


ATOM
997
CG
ARG
A
131
−6.550
1.438
−29.566
1.00
15.60


ATOM
998
CD
ARG
A
131
−5.278
1.522
−30.428
1.00
15.59


ATOM
999
NE
ARG
A
131
−4.118
0.915
−29.779
1.00
16.77


ATOM
1000
CZ
ARG
A
131
−2.860
1.205
−30.098
1.00
16.26


ATOM
1001
NH1
ARG
A
131
−2.610
2.104
−31.054
1.00
16.37


ATOM
1002
NH2
ARG
A
131
−1.856
0.618
−29.448
1.00
15.96


ATOM
1003
C
ARG
A
131
−8.408
0.729
−27.283
1.00
14.44


ATOM
1004
O
ARG
A
131
−9.350
0.533
−28.050
1.00
15.71


ATOM
1005
N
ALA
A
132
−8.025
−0.164
−26.364
1.00
14.50


ATOM
1006
CA
ALA
A
132
−8.738
−1.456
−26.195
1.00
15.06


ATOM
1007
CB
ALA
A
132
−8.069
−2.348
−25.112
1.00
15.02


ATOM
1008
C
ALA
A
132
−10.194
−1.197
−25.846
1.00
15.43


ATOM
1009
O
ALA
A
132
−11.101
−1.808
−26.416
1.00
15.57


ATOM
1010
N
ILE
A
133
−10.418
−0.270
−24.915
1.00
15.70


ATOM
1011
CA
ILE
A
133
−11.777
0.049
−24.491
1.00
14.44


ATOM
1012
CB
ILE
A
133
−11.775
1.056
−23.335
1.00
14.07


ATOM
1013
CG1
ILE
A
133
−11.268
0.387
−22.046
1.00
15.40


ATOM
1014
CD1
ILE
A
133
−10.751
1.388
−21.017
1.00
16.66


ATOM
1015
CG2
ILE
A
133
−13.176
1.702
−23.101
1.00
13.98


ATOM
1016
C
ILE
A
133
−12.633
0.517
−25.679
1.00
14.36


ATOM
1017
O
ILE
A
133
−13.781
0.102
−25.807
1.00
14.69


ATOM
1018
N
ALA
A
134
−12.079
1.362
−26.545
1.00
13.69


ATOM
1019
CA
ALA
A
134
−12.819
1.832
−27.720
1.00
13.81


ATOM
1020
CB
ALA
A
134
−12.019
2.949
−28.452
1.00
13.99


ATOM
1021
C
ALA
A
134
−13.140
0.662
−28.657
1.00
14.62


ATOM
1022
O
ALA
A
134
−14.279
0.473
−29.087
1.00
14.90


ATOM
1023
N
LEU
A
135
−12.133
−0.155
−28.947
1.00
14.66


ATOM
1024
CA
LEU
A
135
−12.328
−1.251
−29.901
1.00
15.75


ATOM
1025
CB
LEU
A
135
−10.984
−1.875
−30.311
1.00
15.75


ATOM
1026
CG
LEU
A
135
−10.348
−1.231
−31.557
1.00
16.40


ATOM
1027
CD1
LEU
A
135
−10.257
0.308
−31.471
1.00
18.56


ATOM
1028
CD2
LEU
A
135
−8.980
−1.852
−31.804
1.00
17.85


ATOM
1029
C
LEU
A
135
−13.277
−2.306
−29.340
1.00
15.13


ATOM
1030
O
LEU
A
135
−14.079
−2.845
−30.087
1.00
15.84


ATOM
1031
N
ILE
A
136
−13.192
−2.573
−28.039
1.00
15.22


ATOM
1032
CA
ILE
A
136
−14.153
−3.473
−27.377
1.00
15.71


ATOM
1033
CB
ILE
A
136
−13.734
−3.829
−25.918
1.00
15.92


ATOM
1034
CG1
ILE
A
136
−12.408
−4.598
−25.904
1.00
15.39


ATOM
1035
CD1
ILE
A
136
−11.742
−4.679
−24.497
1.00
15.63


ATOM
1036
CG2
ILE
A
136
−14.842
−4.611
−25.204
1.00
16.20


ATOM
1037
C
ILE
A
136
−15.565
−2.902
−27.457
1.00
17.10


ATOM
1038
O
ILE
A
136
−16.531
−3.631
−27.728
1.00
17.48


ATOM
1039
N
GLY
A
137
−15.685
−1.581
−27.297
1.00
16.46


ATOM
1040
CA
GLY
A
137
−16.979
−0.902
−27.484
1.00
16.59


ATOM
1041
C
GLY
A
137
−17.600
−1.206
−28.833
1.00
17.13


ATOM
1042
O
GLY
A
137
−18.778
−1.605
−28.920
1.00
16.92


ATOM
1043
N
TYR
A
138
−16.817
−1.056
−29.898
1.00
16.61


ATOM
1044
CA
TYR
A
138
−17.353
−1.349
−31.224
1.00
17.93


ATOM
1045
CB
TYR
A
138
−16.446
−0.838
−32.341
1.00
17.46


ATOM
1046
CG
TYR
A
138
−17.112
−0.897
−33.693
1.00
18.28


ATOM
1047
CD1
TYR
A
138
−18.350
−0.268
−33.914
1.00
18.85


ATOM
1048
CE1
TYR
A
138
−18.966
−0.314
−35.153
1.00
21.29


ATOM
1049
CZ
TYR
A
138
−18.358
−1.001
−36.207
1.00
20.99


ATOM
1050
OH
TYR
A
138
−18.994
−1.055
−37.433
1.00
20.06


ATOM
1051
CE2
TYR
A
138
−17.133
−1.636
−36.026
1.00
19.88


ATOM
1052
CD2
TYR
A
138
−16.512
−1.583
−34.766
1.00
18.25


ATOM
1053
C
TYR
A
138
−17.643
−2.844
−31.406
1.00
18.67


ATOM
1054
O
TYR
A
138
−18.654
−3.207
−32.037
1.00
20.06


ATOM
1055
N
SER
A
139
−16.766
−3.686
−30.864
1.00
19.48


ATOM
1056
CA
SER
A
139
−16.942
−5.146
−30.900
1.00
21.02


ATOM
1057
CB
SER
A
139
−15.808
−5.829
−30.129
1.00
21.09


ATOM
1058
OG
SER
A
139
−14.581
−5.598
−30.789
1.00
21.49


ATOM
1059
C
SER
A
139
−18.298
−5.557
−30.325
1.00
22.24


ATOM
1060
O
SER
A
139
−19.002
−6.392
−30.907
1.00
23.64


ATOM
1061
N
LYS
A
140
−18.669
−4.976
−29.188
1.00
22.77


ATOM
1062
CA
LYS
A
140
−19.987
−5.225
−28.595
1.00
24.29


ATOM
1063
CB
LYS
A
140
−20.218
−4.343
−27.370
1.00
24.29


ATOM
1064
CG
LYS
A
140
−19.384
−4.695
−26.170
1.00
26.62


ATOM
1065
CD
LYS
A
140
−19.696
−3.693
−25.060
1.00
28.24


ATOM
1066
CE
LYS
A
140
−18.589
−3.635
−24.056
1.00
28.19


ATOM
1067
NZ
LYS
A
140
−18.940
−2.725
−22.954
1.00
26.78


ATOM
1068
C
LYS
A
140
−21.126
−5.001
−29.584
1.00
24.17


ATOM
1069
O
LYS
A
140
−22.053
−5.823
−29.670
1.00
24.94


ATOM
1070
N
TRP
A
141
−21.062
−3.898
−30.321
1.00
23.60


ATOM
1071
CA
TRP
A
141
−22.054
−3.613
−31.338
1.00
24.29


ATOM
1072
CB
TRP
A
141
−21.847
−2.226
−31.953
1.00
24.36


ATOM
1073
CG
TRP
A
141
−22.973
−1.833
−32.874
1.00
24.25


ATOM
1074
CD1
TRP
A
141
−24.113
−1.170
−32.531
1.00
25.06


ATOM
1075
NE1
TRP
A
141
−24.921
−1.016
−33.638
1.00
25.22


ATOM
1076
CE2
TRP
A
141
−24.302
−1.575
−34.722
1.00
24.07


ATOM
1077
CD2
TRP
A
141
−23.078
−2.115
−34.276
1.00
24.80


ATOM
1078
CE3
TRP
A
141
−22.248
−2.766
−35.203
1.00
25.40


ATOM
1079
CZ3
TRP
A
141
−22.669
−2.858
−36.532
1.00
25.72


ATOM
1080
CH2
TRP
A
141
−23.891
−2.304
−36.940
1.00
24.95


ATOM
1081
CZ2
TRP
A
141
−24.721
−1.666
−36.051
1.00
25.14


ATOM
1082
C
TRP
A
141
−22.078
−4.666
−32.448
1.00
24.47


ATOM
1083
O
TRP
A
141
−23.155
−5.152
−32.831
1.00
24.52


ATOM
1084
N
LEU
A
142
−20.904
−4.991
−32.985
1.00
24.39


ATOM
1085
CA
LEU
A
142
−20.806
−6.024
−34.010
1.00
25.05


ATOM
1086
CB
LEU
A
142
−19.361
−6.199
−34.473
1.00
24.56


ATOM
1087
CG
LEU
A
142
−18.754
−5.023
−35.252
1.00
24.44


ATOM
1088
CD1
LEU
A
142
−17.274
−5.304
−35.441
1.00
23.72


ATOM
1089
CD2
LEU
A
142
−19.424
−4.793
−36.624
1.00
25.76


ATOM
1090
C
LEU
A
142
−21.406
−7.364
−33.556
1.00
25.97


ATOM
1091
O
LEU
A
142
−22.195
−7.966
−34.283
1.00
26.49


ATOM
1092
N
ILE
A
143
−21.045
−7.814
−32.359
1.00
27.04


ATOM
1093
CA
ILE
A
143
−21.596
−9.040
−31.792
1.00
28.46


ATOM
1094
CB
ILE
A
143
−20.959
−9.362
−30.425
1.00
28.30


ATOM
1095
CG1
ILE
A
143
−19.474
−9.722
−30.609
1.00
27.97


ATOM
1096
CD1
ILE
A
143
−18.707
−9.814
−29.301
1.00
29.85


ATOM
1097
CG2
ILE
A
143
−21.720
−10.494
−29.717
1.00
29.57


ATOM
1098
C
ILE
A
143
−23.124
−8.992
−31.682
1.00
29.52


ATOM
1099
O
ILE
A
143
−23.813
−9.928
−32.118
1.00
30.10


ATOM
1100
N
ASN
A
144
−23.655
−7.916
−31.111
1.00
30.37


ATOM
1101
CA
ASN
A
144
−25.109
−7.768
−30.988
1.00
32.18


ATOM
1102
CB
ASN
A
144
−25.479
−6.522
−30.186
1.00
32.73


ATOM
1103
CG
ASN
A
144
−26.960
−6.489
−29.792
1.00
36.88


ATOM
1104
OD1
ASN
A
144
−27.444
−7.350
−29.041
1.00
42.25


ATOM
1105
ND2
ASN
A
144
−27.685
−5.488
−30.291
1.00
40.10


ATOM
1106
C
ASN
A
144
−25.820
−7.760
−32.341
1.00
32.17


ATOM
1107
O
ASN
A
144
−27.012
−8.029
−32.411
1.00
32.73


ATOM
1108
N
ASN
A
145
−25.094
−7.460
−33.411
1.00
32.19


ATOM
1109
CA
ASN
A
145
−25.705
−7.403
−34.726
1.00
32.78


ATOM
1110
CB
ASN
A
145
−25.526
−6.014
−35.331
1.00
33.16


ATOM
1111
CG
ASN
A
145
−26.397
−4.986
−34.639
1.00
34.32


ATOM
1112
OD1
ASN
A
145
−27.576
−4.841
−34.969
1.00
37.42


ATOM
1113
ND2
ASN
A
145
−25.834
−4.289
−33.647
1.00
34.31


ATOM
1114
C
ASN
A
145
−25.285
−8.533
−35.671
1.00
33.13


ATOM
1115
O
ASN
A
145
−25.412
−8.415
−36.902
1.00
33.03


ATOM
1116
N
ASN
A
146
−24.789
−9.618
−35.065
1.00
33.43


ATOM
1117
CA
ASN
A
146
−24.475
−10.885
−35.736
1.00
34.14


ATOM
1118
CB
ASN
A
146
−25.710
−11.459
−36.461
1.00
34.85


ATOM
1119
CG
ASN
A
146
−26.994
−11.280
−35.657
1.00
37.41


ATOM
1120
OD1
ASN
A
146
−27.033
−11.543
−34.450
1.00
41.43


ATOM
1121
ND2
ASN
A
146
−28.047
−10.814
−36.321
1.00
41.54


ATOM
1122
C
ASN
A
146
−23.266
−10.795
−36.652
1.00
33.92


ATOM
1123
O
ASN
A
146
−23.216
−11.419
−37.724
1.00
33.76


ATOM
1124
N
TYR
A
147
−22.280
−10.013
−36.221
1.00
32.92


ATOM
1125
CA
TYR
A
147
−21.049
−9.870
−36.974
1.00
32.90


ATOM
1126
CB
TYR
A
147
−20.859
−8.423
−37.451
1.00
33.31


ATOM
1127
CG
TYR
A
147
−21.966
−7.893
−38.339
1.00
33.29


ATOM
1128
CD1
TYR
A
147
−22.168
−8.410
−39.621
1.00
34.11


ATOM
1129
CE1
TYR
A
147
−23.177
−7.924
−40.438
1.00
34.19


ATOM
1130
CZ
TYR
A
147
−23.986
−6.888
−39.983
1.00
34.10


ATOM
1131
OH
TYR
A
147
−24.987
−6.399
−40.794
1.00
35.08


ATOM
1132
CE2
TYR
A
147
−23.798
−6.345
−38.722
1.00
32.48


ATOM
1133
CD2
TYR
A
147
−22.792
−6.852
−37.906
1.00
32.03


ATOM
1134
C
TYR
A
147
−19.857
−10.297
−36.138
1.00
32.74


ATOM
1135
O
TYR
A
147
−18.795
−9.710
−36.242
1.00
32.03


ATOM
1136
N
GLN
A
148
−20.037
−11.325
−35.312
1.00
33.44


ATOM
1137
CA
GLN
A
148
−18.977
−11.807
−34.427
1.00
34.39


ATOM
1138
CB
GLN
A
148
−19.483
−12.971
−33.573
1.00
34.76


ATOM
1139
CG
GLN
A
148
−18.523
−13.445
−32.481
1.00
35.56


ATOM
1140
CD
GLN
A
148
−19.216
−14.273
−31.401
1.00
36.59


ATOM
1141
OE1
GLN
A
148
−20.296
−13.913
−30.916
1.00
41.20


ATOM
1142
NE2
GLN
A
148
−18.589
−15.380
−31.008
1.00
38.65


ATOM
1143
C
GLN
A
148
−17.690
−12.196
−35.176
1.00
34.46


ATOM
1144
O
GLN
A
148
−16.582
−12.002
−34.654
1.00
34.20


ATOM
1145
N
PHE
A
149
−17.841
−12.735
−36.391
1.00
34.19


ATOM
1146
CA
PHE
A
149
−16.696
−13.131
−37.217
1.00
34.10


ATOM
1147
CB
PHE
A
149
−17.140
−13.804
−38.534
1.00
35.60


ATOM
1148
CG
PHE
A
149
−18.346
−13.168
−39.193
1.00
38.70


ATOM
1149
CD1
PHE
A
149
−19.388
−13.976
−39.676
1.00
42.72


ATOM
1150
CE1
PHE
A
149
−20.518
−13.417
−40.295
1.00
43.69


ATOM
1151
CZ
PHE
A
149
−20.615
−12.019
−40.437
1.00
43.12


ATOM
1152
CE2
PHE
A
149
−19.567
−11.188
−39.953
1.00
43.20


ATOM
1153
CD2
PHE
A
149
−18.451
−11.772
−39.341
1.00
42.33


ATOM
1154
C
PHE
A
149
−15.746
−11.960
−37.517
1.00
32.49


ATOM
1155
O
PHE
A
149
−14.528
−12.132
−37.548
1.00
32.23


ATOM
1156
N
THR
A
150
−16.327
−10.789
−37.751
1.00
30.95


ATOM
1157
CA
THR
A
150
−15.570
−9.568
−38.040
1.00
29.68


ATOM
1158
CB
THR
A
150
−16.512
−8.424
−38.445
1.00
29.97


ATOM
1159
OG1
THR
A
150
−17.162
−8.768
−39.673
1.00
30.58


ATOM
1160
CG2
THR
A
150
−15.758
−7.096
−38.637
1.00
29.18


ATOM
1161
C
THR
A
150
−14.727
−9.203
−36.822
1.00
28.54


ATOM
1162
O
THR
A
150
−13.566
−8.827
−36.965
1.00
28.38


ATOM
1163
N
VAL
A
151
−15.310
−9.354
−35.636
1.00
27.23


ATOM
1164
CA
VAL
A
151
−14.597
−9.146
−34.374
1.00
26.78


ATOM
1165
CB
VAL
A
151
−15.529
−9.352
−33.148
1.00
26.25


ATOM
1166
CG1
VAL
A
151
−14.752
−9.213
−31.832
1.00
26.50


ATOM
1167
CG2
VAL
A
151
−16.690
−8.361
−33.178
1.00
24.41


ATOM
1168
C
VAL
A
151
−13.384
−10.080
−34.305
1.00
27.61


ATOM
1169
O
VAL
A
151
−12.246
−9.638
−34.106
1.00
26.67


ATOM
1170
N
SER
A
152
−13.625
−11.375
−34.505
1.00
28.53


ATOM
1171
CA
SER
A
152
−12.551
−12.369
−34.470
1.00
30.01


ATOM
1172
CB
SER
A
152
−13.102
−13.759
−34.773
1.00
30.18


ATOM
1173
OG
SER
A
152
−13.612
−14.300
−33.586
1.00
32.28


ATOM
1174
C
SER
A
152
−11.419
−12.091
−35.430
1.00
30.23


ATOM
1175
O
SER
A
152
−10.250
−12.250
−35.090
1.00
30.95


ATOM
1176
N
ASN
A
153
−11.762
−11.705
−36.641
1.00
31.46


ATOM
1177
CA
ASN
A
153
−10.753
−11.624
−37.674
1.00
32.41


ATOM
1178
CB
ASN
A
153
−11.333
−12.118
−38.997
1.00
33.62


ATOM
1179
CG
ASN
A
153
−11.791
−13.584
−38.902
1.00
36.13


ATOM
1180
OD1
ASN
A
153
−12.931
−13.918
−39.231
1.00
40.99


ATOM
1181
ND2
ASN
A
153
−10.917
−14.444
−38.383
1.00
37.61


ATOM
1182
C
ASN
A
153
−10.060
−10.272
−37.787
1.00
32.10


ATOM
1183
O
ASN
A
153
−8.850
−10.213
−38.020
1.00
32.79


ATOM
1184
N
VAL
A
154
−10.810
−9.193
−37.577
1.00
30.55


ATOM
1185
CA
VAL
A
154
−10.251
−7.854
−37.750
1.00
28.93


ATOM
1186
CB
VAL
A
154
−11.217
−6.925
−38.537
1.00
29.03


ATOM
1187
CG1
VAL
A
154
−10.565
−5.577
−38.827
1.00
29.23


ATOM
1188
CG2
VAL
A
154
−11.654
−7.585
−39.860
1.00
29.87


ATOM
1189
C
VAL
A
154
−9.824
−7.211
−36.414
1.00
27.28


ATOM
1190
O
VAL
A
154
−8.722
−6.678
−36.306
1.00
26.96


ATOM
1191
N
ILE
A
155
−10.685
−7.288
−35.403
1.00
25.13


ATOM
1192
CA
ILE
A
155
−10.525
−6.459
−34.197
1.00
23.05


ATOM
1193
CB
ILE
A
155
−11.900
−5.972
−33.670
1.00
23.10


ATOM
1194
CG1
ILE
A
155
−12.596
−5.128
−34.741
1.00
22.49


ATOM
1195
CD1
ILE
A
155
−14.006
−4.680
−34.375
1.00
22.72


ATOM
1196
CG2
ILE
A
155
−11.731
−5.144
−32.399
1.00
23.05


ATOM
1197
C
ILE
A
155
−9.710
−7.136
−33.092
1.00
22.44


ATOM
1198
O
ILE
A
155
−8.789
−6.537
−32.533
1.00
21.14


ATOM
1199
N
TRP
A
156
−10.006
−8.409
−32.822
1.00
21.48


ATOM
1200
CA
TRP
A
156
−9.392
−9.099
−31.696
1.00
21.75


ATOM
1201
CB
TRP
A
156
−9.958
−10.520
−31.511
1.00
22.50


ATOM
1202
CG
TRP
A
156
−9.298
−11.245
−30.371
1.00
23.43


ATOM
1203
CD1
TRP
A
156
−8.420
−12.298
−30.461
1.00
24.92


ATOM
1204
NE1
TRP
A
156
−8.011
−12.680
−29.198
1.00
24.85


ATOM
1205
CE2
TRP
A
156
−8.600
−11.863
−28.269
1.00
26.38


ATOM
1206
CD2
TRP
A
156
−9.416
−10.941
−28.970
1.00
25.03


ATOM
1207
CE3
TRP
A
156
−10.139
−9.983
−28.236
1.00
25.01


ATOM
1208
CZ3
TRP
A
156
−10.024
−9.982
−26.844
1.00
24.30


ATOM
1209
CH2
TRP
A
156
−9.206
−10.910
−26.185
1.00
23.97


ATOM
1210
CZ2
TRP
A
156
−8.495
−11.861
−26.875
1.00
24.60


ATOM
1211
C
TRP
A
156
−7.845
−9.109
−31.699
1.00
21.52


ATOM
1212
O
TRP
A
156
−7.235
−8.945
−30.648
1.00
21.78


ATOM
1213
N
PRO
A
157
−7.209
−9.303
−32.870
1.00
21.66


ATOM
1214
CA
PRO
A
157
−5.726
−9.258
−32.878
1.00
21.40


ATOM
1215
CB
PRO
A
157
−5.378
−9.459
−34.360
1.00
21.26


ATOM
1216
CG
PRO
A
157
−6.583
−10.172
−34.955
1.00
22.82


ATOM
1217
CD
PRO
A
157
−7.762
−9.596
−34.207
1.00
21.45


ATOM
1218
C
PRO
A
157
−5.162
−7.898
−32.410
1.00
21.30


ATOM
1219
O
PRO
A
157
−4.092
−7.837
−31.795
1.00
21.11


ATOM
1220
N
ILE
A
158
−5.881
−6.821
−32.724
1.00
20.52


ATOM
1221
CA
ILE
A
158
−5.457
−5.467
−32.318
1.00
19.93


ATOM
1222
CB
ILE
A
158
−6.273
−4.348
−33.034
1.00
19.73


ATOM
1223
CG1
ILE
A
158
−6.261
−4.527
−34.559
1.00
21.25


ATOM
1224
CD1
ILE
A
158
−7.229
−3.640
−35.351
1.00
20.03


ATOM
1225
CG2
ILE
A
158
−5.686
−2.971
−32.670
1.00
20.16


ATOM
1226
C
ILE
A
158
−5.632
−5.366
−30.816
1.00
19.58


ATOM
1227
O
ILE
A
158
−4.701
−5.023
−30.081
1.00
19.04


ATOM
1228
N
VAL
A
159
−6.840
−5.704
−30.359
1.00
19.39


ATOM
1229
CA
VAL
A
159
−7.201
−5.624
−28.953
1.00
19.15


ATOM
1230
CB
VAL
A
159
−8.687
−6.026
−28.744
1.00
19.06


ATOM
1231
CG1
VAL
A
159
−9.046
−6.028
−27.253
1.00
20.39


ATOM
1232
CG2
VAL
A
159
−9.604
−5.090
−29.511
1.00
20.08


ATOM
1233
C
VAL
A
159
−6.280
−6.501
−28.105
1.00
19.39


ATOM
1234
O
VAL
A
159
−5.794
−6.089
−27.036
1.00
18.63


ATOM
1235
N
ARG
A
160
−6.022
−7.721
−28.585
1.00
18.93


ATOM
1236
CA
ARG
A
160
−5.171
−8.633
−27.833
1.00
19.64


ATOM
1237
CB
ARG
A
160
−5.078
−10.005
−28.513
1.00
19.17


ATOM
1238
CG
ARG
A
160
−4.064
−10.942
−27.872
1.00
21.41


ATOM
1239
CD
ARG
A
160
−3.978
−12.278
−28.637
1.00
23.47


ATOM
1240
NE
ARG
A
160
−3.542
−12.066
−30.021
1.00
29.25


ATOM
1241
CZ
ARG
A
160
−3.963
−12.771
−31.074
1.00
33.46


ATOM
1242
NH1
ARG
A
160
−4.839
−13.764
−30.929
1.00
36.48


ATOM
1243
NH2
ARG
A
160
−3.501
−12.489
−32.289
1.00
34.33


ATOM
1244
C
ARG
A
160
−3.785
−8.058
−27.580
1.00
18.24


ATOM
1245
O
ARG
A
160
−3.262
−8.233
−26.517
1.00
18.13


ATOM
1246
N
ASN
A
161
−3.182
−7.371
−28.551
1.00
18.57


ATOM
1247
CA
ASN
A
161
−1.875
−6.717
−28.289
1.00
18.40


ATOM
1248
CB
ASN
A
161
−1.344
−6.052
−29.561
1.00
18.82


ATOM
1249
CG
ASN
A
161
−0.772
−7.055
−30.549
1.00
20.68


ATOM
1250
OD1
ASN
A
161
−0.240
−8.097
−30.149
1.00
22.60


ATOM
1251
ND2
ASN
A
161
−0.883
−6.751
−31.837
1.00
19.81


ATOM
1252
C
ASN
A
161
−1.946
−5.656
−27.182
1.00
18.36


ATOM
1253
O
ASN
A
161
−1.078
−5.581
−26.313
1.00
17.42


ATOM
1254
N
ASP
A
162
−2.982
−4.816
−27.233
1.00
17.86


ATOM
1255
CA
ASP
A
162
−3.163
−3.782
−26.194
1.00
16.85


ATOM
1256
CB
ASP
A
162
−4.293
−2.821
−26.586
1.00
16.71


ATOM
1257
CG
ASP
A
162
−3.851
−1.791
−27.623
1.00
17.46


ATOM
1258
OD1
ASP
A
162
−2.648
−1.440
−27.681
1.00
16.98


ATOM
1259
OD2
ASP
A
162
−4.719
−1.333
−28.388
1.00
18.89


ATOM
1260
C
ASP
A
162
−3.421
−4.354
−24.799
1.00
16.51


ATOM
1261
O
ASP
A
162
−2.846
−3.897
−23.822
1.00
15.71


ATOM
1262
N
LEU
A
163
−4.278
−5.371
−24.715
1.00
16.83


ATOM
1263
CA
LEU
A
163
−4.532
−6.071
−23.459
1.00
16.55


ATOM
1264
CB
LEU
A
163
−5.661
−7.088
−23.637
1.00
16.96


ATOM
1265
CG
LEU
A
163
−7.030
−6.506
−23.975
1.00
19.29


ATOM
1266
CD1
LEU
A
163
−8.007
−7.663
−24.227
1.00
19.71


ATOM
1267
CD2
LEU
A
163
−7.484
−5.631
−22.818
1.00
21.19


ATOM
1268
C
LEU
A
163
−3.279
−6.750
−22.883
1.00
16.19


ATOM
1269
O
LEU
A
163
−3.035
−6.690
−21.688
1.00
15.47


ATOM
1270
N
ASN
A
164
−2.495
−7.401
−23.748
1.00
16.85


ATOM
1271
CA
ASN
A
164
−1.251
−8.040
−23.305
1.00
16.44


ATOM
1272
CB
ASN
A
164
−0.602
−8.836
−24.450
1.00
17.06


ATOM
1273
CG
ASN
A
164
−1.333
−10.153
−24.718
1.00
19.63


ATOM
1274
OD1
ASN
A
164
−2.274
−10.513
−23.982
1.00
20.97


ATOM
1275
ND2
ASN
A
164
−0.903
−10.881
−25.756
1.00
19.79


ATOM
1276
C
ASN
A
164
−0.301
−7.022
−22.761
1.00
16.80


ATOM
1277
O
ASN
A
164
0.349
−7.261
−21.751
1.00
15.97


ATOM
1278
N
TYR
A
165
−0.250
−5.860
−23.415
1.00
16.45


ATOM
1279
CA
TYR
A
165
0.573
−4.744
−22.930
1.00
16.15


ATOM
1280
CB
TYR
A
165
0.420
−3.508
−23.844
1.00
16.63


ATOM
1281
CG
TYR
A
165
1.286
−2.356
−23.391
1.00
16.41


ATOM
1282
CD1
TYR
A
165
0.838
−1.459
−22.404
1.00
17.94


ATOM
1283
CE1
TYR
A
165
1.651
−0.402
−21.958
1.00
17.84


ATOM
1284
CZ
TYR
A
165
2.916
−0.223
−22.517
1.00
18.36


ATOM
1285
OH
TYR
A
165
3.699
0.841
−22.091
1.00
16.54


ATOM
1286
CE2
TYR
A
165
3.383
−1.105
−23.502
1.00
16.73


ATOM
1287
CD2
TYR
A
165
2.552
−2.157
−23.942
1.00
16.01


ATOM
1288
C
TYR
A
165
0.198
−4.366
−21.503
1.00
16.26


ATOM
1289
O
TYR
A
165
1.073
−4.218
−20.650
1.00
15.43


ATOM
1290
N
VAL
A
166
−1.104
−4.177
−21.258
1.00
16.75


ATOM
1291
CA
VAL
A
166
−1.600
−3.786
−19.933
1.00
17.39


ATOM
1292
CB
VAL
A
166
−3.124
−3.479
−19.986
1.00
17.57


ATOM
1293
CG1
VAL
A
166
−3.712
−3.197
−18.582
1.00
19.25


ATOM
1294
CG2
VAL
A
166
−3.363
−2.272
−20.909
1.00
16.49


ATOM
1295
C
VAL
A
166
−1.258
−4.829
−18.865
1.00
17.83


ATOM
1296
O
VAL
A
166
−0.741
−4.483
−17.792
1.00
18.00


ATOM
1297
N
ALA
A
167
−1.520
−6.099
−19.188
1.00
18.26


ATOM
1298
CA
ALA
A
167
−1.233
−7.218
−18.285
1.00
18.92


ATOM
1299
CB
ALA
A
167
−1.716
−8.532
−18.899
1.00
18.25


ATOM
1300
C
ALA
A
167
0.251
−7.325
−17.956
1.00
19.08


ATOM
1301
O
ALA
A
167
0.611
−7.757
−16.854
1.00
20.02


ATOM
1302
N
GLN
A
168
1.097
−6.955
−18.920
1.00
19.13


ATOM
1303
CA
GLN
A
168
2.558
−7.022
−18.749
1.00
19.21


ATOM
1304
CB
GLN
A
168
3.218
−7.201
−20.115
1.00
19.08


ATOM
1305
CG
GLN
A
168
4.739
−7.373
−20.053
1.00
20.55


ATOM
1306
CD
GLN
A
168
5.337
−7.891
−21.355
1.00
20.26


ATOM
1307
OE1
GLN
A
168
4.634
−8.378
−22.238
1.00
22.69


ATOM
1308
NE2
GLN
A
168
6.643
−7.772
−21.476
1.00
23.10


ATOM
1309
C
GLN
A
168
3.182
−5.807
−18.048
1.00
19.60


ATOM
1310
O
GLN
A
168
4.104
−5.942
−17.205
1.00
18.87


ATOM
1311
N
TYR
A
169
2.709
−4.609
−18.404
1.00
19.23


ATOM
1312
CA
TYR
A
169
3.399
−3.377
−18.011
1.00
19.45


ATOM
1313
CB
TYR
A
169
3.760
−2.560
−19.266
1.00
20.43


ATOM
1314
CG
TYR
A
169
4.773
−3.203
−20.203
1.00
21.30


ATOM
1315
CD1
TYR
A
169
6.125
−3.243
−19.872
1.00
23.63


ATOM
1316
CE1
TYR
A
169
7.065
−3.822
−20.723
1.00
24.59


ATOM
1317
CZ
TYR
A
169
6.651
−4.359
−21.926
1.00
23.11


ATOM
1318
OH
TYR
A
169
7.580
−4.924
−22.779
1.00
25.26


ATOM
1319
CE2
TYR
A
169
5.309
−4.330
−22.288
1.00
22.49


ATOM
1320
CD2
TYR
A
169
4.375
−3.754
−21.422
1.00
20.87


ATOM
1321
C
TYR
A
169
2.675
−2.449
−17.015
1.00
19.49


ATOM
1322
O
TYR
A
169
3.205
−1.386
−16.691
1.00
19.69


ATOM
1323
N
TRP
A
170
1.508
−2.850
−16.498
1.00
18.91


ATOM
1324
CA
TRP
A
170
0.735
−1.981
−15.588
1.00
19.29


ATOM
1325
CB
TRP
A
170
−0.610
−2.626
−15.208
1.00
18.85


ATOM
1326
CG
TRP
A
170
−0.489
−3.743
−14.215
1.00
21.04


ATOM
1327
CD1
TRP
A
170
−0.342
−5.083
−14.489
1.00
20.67


ATOM
1328
NE1
TRP
A
170
−0.259
−5.793
−13.317
1.00
22.09


ATOM
1329
CE2
TRP
A
170
−0.336
−4.928
−12.258
1.00
19.19


ATOM
1330
CD2
TRP
A
170
−0.481
−3.621
−12.789
1.00
20.35


ATOM
1331
CE3
TRP
A
170
−0.582
−2.530
−11.905
1.00
19.61


ATOM
1332
CZ3
TRP
A
170
−0.546
−2.769
−10.542
1.00
22.33


ATOM
1333
CH2
TRP
A
170
−0.404
−4.090
−10.038
1.00
21.08


ATOM
1334
CZ2
TRP
A
170
−0.297
−5.179
−10.884
1.00
20.76


ATOM
1335
C
TRP
A
170
1.526
−1.592
−14.336
1.00
19.20


ATOM
1336
O
TRP
A
170
1.395
−0.475
−13.808
1.00
19.24


ATOM
1337
N
ASN
A
171
2.371
−2.504
−13.858
1.00
19.13


ATOM
1338
CA
ASN
A
171
3.054
−2.280
−12.596
1.00
20.12


ATOM
1339
CB
ASN
A
171
3.178
−3.603
−11.820
1.00
20.59


ATOM
1340
CG
ASN
A
171
3.646
−3.419
−10.392
1.00
22.31


ATOM
1341
OD1
ASN
A
171
4.531
−4.155
−9.938
1.00
23.68


ATOM
1342
ND2
ASN
A
171
3.081
−2.435
−9.684
1.00
18.77


ATOM
1343
C
ASN
A
171
4.392
−1.557
−12.797
1.00
20.93


ATOM
1344
O
ASN
A
171
5.333
−1.724
−12.022
1.00
20.35


ATOM
1345
N
GLN
A
172
4.449
−0.712
−13.826
1.00
20.64


ATOM
1346
CA
GLN
A
172
5.644
0.061
−14.156
1.00
22.31


ATOM
1347
CB
GLN
A
172
6.262
−0.452
−15.469
1.00
22.04


ATOM
1348
CG
GLN
A
172
6.784
−1.895
−15.312
1.00
25.79


ATOM
1349
CD
GLN
A
172
7.536
−2.450
−16.515
1.00
27.61


ATOM
1350
OE1
GLN
A
172
8.276
−1.735
−17.214
1.00
35.65


ATOM
1351
NE2
GLN
A
172
7.367
−3.755
−16.752
1.00
33.80


ATOM
1352
C
GLN
A
172
5.287
1.539
−14.268
1.00
21.36


ATOM
1353
O
GLN
A
172
4.175
1.867
−14.704
1.00
21.04


ATOM
1354
N
THR
A
173
6.209
2.417
−13.871
1.00
19.73


ATOM
1355
CA
THR
A
173
5.948
3.871
−13.928
1.00
20.07


ATOM
1356
CB
THR
A
173
7.001
4.703
−13.168
1.00
19.48


ATOM
1357
OG1
THR
A
173
8.300
4.427
−13.707
1.00
21.56


ATOM
1358
CG2
THR
A
173
6.988
4.375
−11.690
1.00
20.86


ATOM
1359
C
THR
A
173
5.913
4.347
−15.375
1.00
18.53


ATOM
1360
O
THR
A
173
6.395
3.665
−16.292
1.00
18.48


ATOM
1361
N
GLY
A
174
5.345
5.528
−15.582
1.00
18.38


ATOM
1362
CA
GLY
A
174
5.363
6.149
−16.903
1.00
17.13


ATOM
1363
C
GLY
A
174
4.760
7.522
−16.736
1.00
16.77


ATOM
1364
O
GLY
A
174
4.462
7.939
−15.605
1.00
16.87


ATOM
1365
N
PHE
A
175
4.571
8.223
−17.849
1.00
14.68


ATOM
1366
CA
PHE
A
175
4.004
9.577
−17.776
1.00
14.68


ATOM
1367
CB
PHE
A
175
4.522
10.432
−18.948
1.00
15.16


ATOM
1368
CG
PHE
A
175
5.943
10.847
−18.756
1.00
15.28


ATOM
1369
CD1
PHE
A
175
6.981
10.000
−19.144
1.00
18.04


ATOM
1370
CE1
PHE
A
175
8.313
10.359
−18.915
1.00
19.53


ATOM
1371
CZ
PHE
A
175
8.609
11.582
−18.278
1.00
19.12


ATOM
1372
CE2
PHE
A
175
7.571
12.429
−17.876
1.00
18.63


ATOM
1373
CD2
PHE
A
175
6.247
12.054
−18.113
1.00
17.67


ATOM
1374
C
PHE
A
175
2.483
9.584
−17.655
1.00
14.11


ATOM
1375
O
PHE
A
175
1.799
8.683
−18.175
1.00
14.32


ATOM
1376
N
ASP
A
176
1.972
10.591
−16.938
1.00
14.79


ATOM
1377
CA
ASP
A
176
0.541
10.764
−16.713
1.00
14.45


ATOM
1378
CB
ASP
A
176
0.297
11.661
−15.506
1.00
13.51


ATOM
1379
CG
ASP
A
176
0.685
13.126
−15.760
1.00
14.99


ATOM
1380
OD1
ASP
A
176
1.774
13.399
−16.329
1.00
14.32


ATOM
1381
OD2
ASP
A
176
−0.112
14.012
−15.376
1.00
15.08


ATOM
1382
C
ASP
A
176
−0.143
11.343
−17.962
1.00
14.21


ATOM
1383
O
ASP
A
176
0.525
11.641
−18.963
1.00
14.31


ATOM
1384
N
LEU
A
177
−1.467
11.511
−17.891
1.00
13.30


ATOM
1385
CA
LEU
A
177
−2.235
11.981
−19.048
1.00
13.44


ATOM
1386
CB
LEU
A
177
−3.752
11.839
−18.832
1.00
13.71


ATOM
1387
CG
LEU
A
177
−4.483
12.896
−18.012
1.00
14.11


ATOM
1388
CD1
LEU
A
177
−5.996
12.647
−18.061
1.00
13.65


ATOM
1389
CD2
LEU
A
177
−4.007
12.922
−16.553
1.00
14.74


ATOM
1390
C
LEU
A
177
−1.884
13.424
−19.452
1.00
13.51


ATOM
1391
O
LEU
A
177
−2.131
13.813
−20.600
1.00
13.74


ATOM
1392
N
TRP
A
178
−1.319
14.206
−18.521
1.00
12.53


ATOM
1393
CA
TRP
A
178
−0.804
15.553
−18.855
1.00
12.95


ATOM
1394
CB
TRP
A
178
−0.890
16.507
−17.660
1.00
12.67


ATOM
1395
CG
TRP
A
178
−2.247
16.549
−17.005
1.00
13.10


ATOM
1396
CD1
TRP
A
178
−2.504
16.508
−15.662
1.00
13.10


ATOM
1397
NE1
TRP
A
178
−3.856
16.568
−15.440
1.00
12.14


ATOM
1398
CE2
TRP
A
178
−4.501
16.646
−16.646
1.00
13.00


ATOM
1399
CD2
TRP
A
178
−3.516
16.641
−17.657
1.00
12.53


ATOM
1400
CE3
TRP
A
178
−3.919
16.715
−19.002
1.00
12.38


ATOM
1401
CZ3
TRP
A
178
−5.309
16.813
−19.290
1.00
13.91


ATOM
1402
CH2
TRP
A
178
−6.262
16.804
−18.257
1.00
13.52


ATOM
1403
CZ2
TRP
A
178
−5.883
16.718
−16.930
1.00
13.97


ATOM
1404
C
TRP
A
178
0.632
15.565
−19.400
1.00
13.35


ATOM
1405
O
TRP
A
178
1.147
16.641
−19.756
1.00
13.76


ATOM
1406
N
GLU
A
179
1.255
14.387
−19.447
1.00
13.33


ATOM
1407
CA
GLU
A
179
2.532
14.151
−20.117
1.00
13.32


ATOM
1408
CB
GLU
A
179
2.503
14.632
−21.582
1.00
12.64


ATOM
1409
CG
GLU
A
179
1.165
14.344
−22.280
1.00
13.03


ATOM
1410
CD
GLU
A
179
1.274
14.434
−23.785
1.00
14.68


ATOM
1411
OE1
GLU
A
179
0.895
15.478
−24.340
1.00
15.98


ATOM
1412
OE2
GLU
A
179
1.730
13.457
−24.405
1.00
15.44


ATOM
1413
C
GLU
A
179
3.667
14.853
−19.374
1.00
15.00


ATOM
1414
O
GLU
A
179
4.626
15.292
−20.004
1.00
14.90


ATOM
1415
N
GLU
A
180
3.561
14.932
−18.048
1.00
14.78


ATOM
1416
CA
GLU
A
180
4.476
15.745
−17.246
1.00
16.76


ATOM
1417
CB
GLU
A
180
3.719
16.928
−16.630
1.00
16.95


ATOM
1418
CG
GLU
A
180
3.282
17.972
−17.654
1.00
18.69


ATOM
1419
CD
GLU
A
180
2.240
18.969
−17.122
1.00
19.72


ATOM
1420
OE1
GLU
A
180
1.587
18.715
−16.077
1.00
19.00


ATOM
1421
OE2
GLU
A
180
2.076
20.020
−17.793
1.00
24.62


ATOM
1422
C
GLU
A
180
5.124
14.954
−16.104
1.00
16.50


ATOM
1423
O
GLU
A
180
6.265
15.202
−15.750
1.00
17.36


ATOM
1424
N
VAL
A
181
4.364
14.056
−15.488
1.00
16.77


ATOM
1425
CA
VAL
A
181
4.775
13.426
−14.218
1.00
16.87


ATOM
1426
CB
VAL
A
181
3.672
13.555
−13.130
1.00
16.78


ATOM
1427
CG1
VAL
A
181
4.030
12.732
−11.893
1.00
18.56


ATOM
1428
CG2
VAL
A
181
3.490
15.008
−12.726
1.00
17.21


ATOM
1429
C
VAL
A
181
5.057
11.953
−14.451
1.00
17.22


ATOM
1430
O
VAL
A
181
4.177
11.205
−14.825
1.00
16.93


ATOM
1431
N
ASN
A
182
6.290
11.532
−14.201
1.00
18.39


ATOM
1432
CA
ASN
A
182
6.674
10.123
−14.394
1.00
18.57


ATOM
1433
CB
ASN
A
182
8.136
10.079
−14.845
1.00
19.77


ATOM
1434
CG
ASN
A
182
8.665
8.669
−15.056
1.00
23.96


ATOM
1435
OD1
ASN
A
182
9.881
8.470
−15.058
1.00
33.20


ATOM
1436
ND2
ASN
A
182
7.794
7.706
−15.258
1.00
23.63


ATOM
1437
C
ASN
A
182
6.440
9.375
−13.073
1.00
18.31


ATOM
1438
O
ASN
A
182
7.132
9.621
−12.087
1.00
18.80


ATOM
1439
N
GLY
A
183
5.436
8.508
−13.034
1.00
16.83


ATOM
1440
CA
GLY
A
183
5.091
7.828
−11.790
1.00
15.98


ATOM
1441
C
GLY
A
183
3.989
6.837
−12.033
1.00
15.59


ATOM
1442
O
GLY
A
183
3.937
6.228
−13.117
1.00
15.30


ATOM
1443
N
SER
A
184
3.119
6.670
−11.035
1.00
14.95


ATOM
1444
CA
SER
A
184
1.927
5.823
−11.151
1.00
15.45


ATOM
1445
CB
SER
A
184
1.844
4.792
−10.017
1.00
15.76


ATOM
1446
OG
SER
A
184
2.998
3.935
−10.027
1.00
17.33


ATOM
1447
C
SER
A
184
0.731
6.758
−11.073
1.00
15.14


ATOM
1448
O
SER
A
184
0.646
7.546
−10.148
1.00
15.83


ATOM
1449
N
SER
A
185
−0.151
6.706
−12.066
1.00
14.73


ATOM
1450
CA
SER
A
185
−1.169
7.755
−12.190
1.00
13.87


ATOM
1451
CB
SER
A
185
−0.991
8.535
−13.515
1.00
14.73


ATOM
1452
OG
SER
A
185
−1.793
9.721
−13.544
1.00
14.93


ATOM
1453
C
SER
A
185
−2.551
7.140
−12.127
1.00
13.49


ATOM
1454
O
SER
A
185
−2.834
6.134
−12.792
1.00
13.35


ATOM
1455
N
PHE
A
186
−3.427
7.782
−11.354
1.00
13.72


ATOM
1456
CA
PHE
A
186
−4.764
7.275
−11.092
1.00
13.43


ATOM
1457
CB
PHE
A
186
−5.511
8.319
−10.260
1.00
13.58


ATOM
1458
CG
PHE
A
186
−6.807
7.839
−9.662
1.00
13.52


ATOM
1459
CD1
PHE
A
186
−6.819
6.873
−8.655
1.00
16.11


ATOM
1460
CE1
PHE
A
186
−8.004
6.489
−8.036
1.00
17.80


ATOM
1461
CZ
PHE
A
186
−9.214
7.062
−8.442
1.00
16.18


ATOM
1462
CE2
PHE
A
186
−9.211
8.051
−9.432
1.00
15.77


ATOM
1463
CD2
PHE
A
186
−8.003
8.435
−10.030
1.00
14.62


ATOM
1464
C
PHE
A
186
−5.552
6.946
−12.372
1.00
13.57


ATOM
1465
O
PHE
A
186
−6.053
5.839
−12.524
1.00
13.36


ATOM
1466
N
PHE
A
187
−5.693
7.927
−13.267
1.00
12.53


ATOM
1467
CA
PHE
A
187
−6.416
7.762
−14.527
1.00
12.84


ATOM
1468
CB
PHE
A
187
−6.284
9.056
−15.356
1.00
11.69


ATOM
1469
CG
PHE
A
187
−6.949
9.016
−16.711
1.00
13.25


ATOM
1470
CD1
PHE
A
187
−8.284
9.338
−16.855
1.00
12.86


ATOM
1471
CE1
PHE
A
187
−8.893
9.342
−18.102
1.00
14.12


ATOM
1472
CZ
PHE
A
187
−8.139
9.041
−19.236
1.00
14.05


ATOM
1473
CE2
PHE
A
187
−6.806
8.721
−19.111
1.00
14.30


ATOM
1474
CD2
PHE
A
187
−6.206
8.711
−17.857
1.00
15.10


ATOM
1475
C
PHE
A
187
−5.887
6.563
−15.318
1.00
12.69


ATOM
1476
O
PHE
A
187
−6.666
5.837
−15.932
1.00
14.00


ATOM
1477
N
THR
A
188
−4.571
6.357
−15.294
1.00
12.97


ATOM
1478
CA
THR
A
188
−3.938
5.302
−16.084
1.00
13.65


ATOM
1479
CB
THR
A
188
−2.411
5.541
−16.104
1.00
13.69


ATOM
1480
OG1
THR
A
188
−2.158
6.789
−16.753
1.00
15.37


ATOM
1481
CG2
THR
A
188
−1.648
4.432
−16.833
1.00
13.24


ATOM
1482
C
THR
A
188
−4.284
3.929
−15.478
1.00
14.12


ATOM
1483
O
THR
A
188
−4.766
3.039
−16.173
1.00
14.40


ATOM
1484
N
VAL
A
189
−4.066
3.798
−14.173
1.00
13.34


ATOM
1485
CA
VAL
A
189
−4.348
2.543
−13.446
1.00
14.76


ATOM
1486
CB
VAL
A
189
−3.893
2.612
−11.958
1.00
14.90


ATOM
1487
CG1
VAL
A
189
−4.331
1.334
−11.186
1.00
16.95


ATOM
1488
CG2
VAL
A
189
−2.374
2.799
−11.865
1.00
15.31


ATOM
1489
C
VAL
A
189
−5.836
2.167
−13.560
1.00
14.48


ATOM
1490
O
VAL
A
189
−6.159
1.024
−13.853
1.00
14.65


ATOM
1491
N
ALA
A
190
−6.732
3.146
−13.372
1.00
13.77


ATOM
1492
CA
ALA
A
190
−8.171
2.858
−13.351
1.00
13.46


ATOM
1493
CB
ALA
A
190
−8.996
4.128
−12.922
1.00
12.74


ATOM
1494
C
ALA
A
190
−8.614
2.388
−14.706
1.00
13.27


ATOM
1495
O
ALA
A
190
−9.432
1.479
−14.815
1.00
13.24


ATOM
1496
N
ASN
A
191
−8.093
3.017
−15.760
1.00
12.50


ATOM
1497
CA
ASN
A
191
−8.438
2.598
−17.127
1.00
13.01


ATOM
1498
CB
ASN
A
191
−8.122
3.707
−18.137
1.00
12.65


ATOM
1499
CG
ASN
A
191
−9.191
4.781
−18.118
1.00
14.08


ATOM
1500
OD1
ASN
A
191
−10.319
4.541
−18.554
1.00
16.39


ATOM
1501
ND2
ASN
A
191
−8.857
5.955
−17.583
1.00
17.21


ATOM
1502
C
ASN
A
191
−7.815
1.259
−17.521
1.00
13.93


ATOM
1503
O
ASN
A
191
−8.412
0.490
−18.270
1.00
13.87


ATOM
1504
N
GLN
A
192
−6.636
0.995
−16.980
1.00
14.06


ATOM
1505
CA
GLN
A
192
−5.988
−0.311
−17.139
1.00
14.96


ATOM
1506
CB
GLN
A
192
−4.575
−0.274
−16.552
1.00
14.33


ATOM
1507
CG
GLN
A
192
−3.555
0.435
−17.500
1.00
13.64


ATOM
1508
CD
GLN
A
192
−2.206
0.635
−16.857
1.00
15.33


ATOM
1509
OE1
GLN
A
192
−2.074
0.568
−15.646
1.00
15.48


ATOM
1510
NE2
GLN
A
192
−1.182
0.925
−17.682
1.00
16.10


ATOM
1511
C
GLN
A
192
−6.855
−1.411
−16.519
1.00
15.11


ATOM
1512
O
GLN
A
192
−7.076
−2.457
−17.141
1.00
16.04


ATOM
1513
N
HIS
A
193
−7.398
−1.140
−15.329
1.00
15.81


ATOM
1514
CA
HIS
A
193
−8.314
−2.069
−14.668
1.00
16.01


ATOM
1515
CB
HIS
A
193
−8.746
−1.586
−13.281
1.00
16.72


ATOM
1516
CG
HIS
A
193
−9.806
−2.454
−12.669
1.00
17.39


ATOM
1517
ND1
HIS
A
193
−11.113
−2.039
−12.505
1.00
18.05


ATOM
1518
CE1
HIS
A
193
−11.821
−3.028
−11.983
1.00
18.70


ATOM
1519
NE2
HIS
A
193
−11.023
−4.071
−11.814
1.00
17.20


ATOM
1520
CD2
HIS
A
193
−9.758
−3.739
−12.242
1.00
18.04


ATOM
1521
C
HIS
A
193
−9.536
−2.343
−15.521
1.00
15.85


ATOM
1522
O
HIS
A
193
−9.898
−3.501
−15.732
1.00
15.70


ATOM
1523
N
ARG
A
194
−10.185
−1.285
−15.995
1.00
15.27


ATOM
1524
CA
ARG
A
194
−11.349
−1.437
−16.852
1.00
15.29


ATOM
1525
CB
ARG
A
194
−11.922
−0.073
−17.234
1.00
14.30


ATOM
1526
CG
ARG
A
194
−13.029
−0.212
−18.239
1.00
14.46


ATOM
1527
CD
ARG
A
194
−13.614
1.102
−18.723
1.00
15.43


ATOM
1528
NE
ARG
A
194
−14.589
0.780
−19.767
1.00
15.58


ATOM
1529
CZ
ARG
A
194
−15.624
1.539
−20.125
1.00
17.92


ATOM
1530
NH1
ARG
A
194
−15.815
2.744
−19.576
1.00
14.60


ATOM
1531
NH2
ARG
A
194
−16.451
1.095
−21.060
1.00
16.15


ATOM
1532
C
ARG
A
194
−11.047
−2.258
−18.111
1.00
15.74


ATOM
1533
O
ARG
A
194
−11.842
−3.120
−18.504
1.00
15.56


ATOM
1534
N
ALA
A
195
−9.918
−1.967
−18.758
1.00
15.60


ATOM
1535
CA
ALA
A
195
−9.562
−2.638
−20.004
1.00
15.90


ATOM
1536
CB
ALA
A
195
−8.254
−2.042
−20.591
1.00
15.40


ATOM
1537
C
ALA
A
195
−9.436
−4.150
−19.798
1.00
15.65


ATOM
1538
O
ALA
A
195
−9.959
−4.929
−20.610
1.00
16.79


ATOM
1539
N
LEU
A
196
−8.763
−4.550
−18.721
1.00
16.36


ATOM
1540
CA
LEU
A
196
−8.552
−5.976
−18.423
1.00
17.02


ATOM
1541
CB
LEU
A
196
−7.625
−6.126
−17.235
1.00
16.96


ATOM
1542
CG
LEU
A
196
−6.167
−5.744
−17.532
1.00
16.96


ATOM
1543
CD1
LEU
A
196
−5.375
−5.857
−16.252
1.00
18.93


ATOM
1544
CD2
LEU
A
196
−5.590
−6.636
−18.630
1.00
20.38


ATOM
1545
C
LEU
A
196
−9.877
−6.685
−18.167
1.00
17.92


ATOM
1546
O
LEU
A
196
−10.102
−7.795
−18.643
1.00
18.98


ATOM
1547
N
VAL
A
197
−10.779
−6.014
−17.454
1.00
18.51


ATOM
1548
CA
VAL
A
197
−12.112
−6.560
−17.181
1.00
18.81


ATOM
1549
CB
VAL
A
197
−12.875
−5.702
−16.130
1.00
18.26


ATOM
1550
CG1
VAL
A
197
−14.340
−6.173
−15.994
1.00
21.18


ATOM
1551
CG2
VAL
A
197
−12.149
−5.784
−14.778
1.00
19.79


ATOM
1552
C
VAL
A
197
−12.924
−6.779
−18.462
1.00
19.05


ATOM
1553
O
VAL
A
197
−13.456
−7.884
−18.693
1.00
18.62


ATOM
1554
N
GLU
A
198
−13.010
−5.752
−19.308
1.00
18.43


ATOM
1555
CA
GLU
A
198
−13.747
−5.873
−20.556
1.00
19.38


ATOM
1556
CB
GLU
A
198
−13.849
−4.517
−21.241
1.00
19.38


ATOM
1557
CG
GLU
A
198
−14.609
−3.530
−20.417
1.00
20.22


ATOM
1558
CD
GLU
A
198
−15.334
−2.537
−21.298
1.00
22.66


ATOM
1559
OE1
GLU
A
198
−16.313
−2.940
−21.940
1.00
22.16


ATOM
1560
OE2
GLU
A
198
−14.924
−1.369
−21.342
1.00
22.92


ATOM
1561
C
GLU
A
198
−13.094
−6.861
−21.509
1.00
19.78


ATOM
1562
O
GLU
A
198
−13.780
−7.506
−22.303
1.00
20.29


ATOM
1563
N
GLY
A
199
−11.770
−6.944
−21.435
1.00
19.78


ATOM
1564
CA
GLY
A
199
−10.998
−7.823
−22.314
1.00
20.88


ATOM
1565
C
GLY
A
199
−11.288
−9.285
−21.986
1.00
21.53


ATOM
1566
O
GLY
A
199
−11.546
−10.083
−22.879
1.00
22.36


ATOM
1567
N
ALA
A
200
−11.256
−9.615
−20.702
1.00
21.79


ATOM
1568
CA
ALA
A
200
−11.605
−10.956
−20.234
1.00
22.44


ATOM
1569
CB
ALA
A
200
−11.463
−11.038
−18.728
1.00
22.21


ATOM
1570
C
ALA
A
200
−13.016
−11.329
−20.696
1.00
22.54


ATOM
1571
O
ALA
A
200
−13.237
−12.419
−21.214
1.00
22.25


ATOM
1572
N
THR
A
201
−13.965
−10.403
−20.573
1.00
22.56


ATOM
1573
CA
THR
A
201
−15.345
−10.671
−20.989
1.00
22.77


ATOM
1574
CB
THR
A
201
−16.302
−9.527
−20.551
1.00
22.83


ATOM
1575
OG1
THR
A
201
−16.219
−9.387
−19.134
1.00
24.92


ATOM
1576
CG2
THR
A
201
−17.756
−9.819
−20.929
1.00
23.76


ATOM
1577
C
THR
A
201
−15.435
−10.905
−22.485
1.00
22.78


ATOM
1578
O
THR
A
201
−16.099
−11.851
−22.925
1.00
22.95


ATOM
1579
N
LEU
A
202
−14.760
−10.069
−23.275
1.00
21.78


ATOM
1580
CA
LEU
A
202
−14.805
−10.236
−24.717
1.00
22.62


ATOM
1581
CB
LEU
A
202
−14.149
−9.055
−25.434
1.00
22.14


ATOM
1582
CG
LEU
A
202
−14.142
−9.107
−26.964
1.00
23.10


ATOM
1583
CD1
LEU
A
202
−15.544
−9.198
−27.564
1.00
24.20


ATOM
1584
CD2
LEU
A
202
−13.346
−7.938
−27.570
1.00
22.53


ATOM
1585
C
LEU
A
202
−14.139
−11.552
−25.151
1.00
23.15


ATOM
1586
O
LEU
A
202
−14.649
−12.245
−26.036
1.00
22.90


ATOM
1587
N
ALA
A
203
−13.019
−11.883
−24.510
1.00
23.38


ATOM
1588
CA
ALA
A
203
−12.300
−13.129
−24.787
1.00
24.07


ATOM
1589
CB
ALA
A
203
−11.076
−13.229
−23.913
1.00
23.57


ATOM
1590
C
ALA
A
203
−13.211
−14.354
−24.569
1.00
24.38


ATOM
1591
O
ALA
A
203
−13.264
−15.244
−25.411
1.00
25.21


ATOM
1592
N
ALA
A
204
−13.920
−14.363
−23.447
1.00
25.20


ATOM
1593
CA
ALA
A
204
−14.849
−15.442
−23.093
1.00
26.63


ATOM
1594
CB
ALA
A
204
−15.450
−15.186
−21.727
1.00
26.12


ATOM
1595
C
ALA
A
204
−15.939
−15.583
−24.150
1.00
27.48


ATOM
1596
O
ALA
A
204
−16.267
−16.687
−24.564
1.00
28.39


ATOM
1597
N
THR
A
205
−16.494
−14.461
−24.593
1.00
27.71


ATOM
1598
CA
THR
A
205
−17.497
−14.470
−25.652
1.00
28.39


ATOM
1599
CB
THR
A
205
−18.088
−13.051
−25.855
1.00
28.42


ATOM
1600
OG1
THR
A
205
−18.669
−12.631
−24.622
1.00
29.32


ATOM
1601
CG2
THR
A
205
−19.150
−13.051
−26.932
1.00
27.32


ATOM
1602
C
THR
A
205
−16.968
−15.004
−26.981
1.00
28.72


ATOM
1603
O
THR
A
205
−17.697
−15.690
−27.719
1.00
29.06


ATOM
1604
N
LEU
A
206
−15.712
−14.698
−27.288
1.00
28.58


ATOM
1605
CA
LEU
A
206
−15.122
−15.122
−28.539
1.00
29.40


ATOM
1606
CB
LEU
A
206
−14.034
−14.144
−29.001
1.00
29.40


ATOM
1607
CG
LEU
A
206
−14.438
−12.694
−29.322
1.00
29.97


ATOM
1608
CD1
LEU
A
206
−13.212
−11.899
−29.755
1.00
30.30


ATOM
1609
CD2
LEU
A
206
−15.561
−12.629
−30.375
1.00
29.58


ATOM
1610
C
LEU
A
206
−14.540
−16.538
−28.489
1.00
29.55


ATOM
1611
O
LEU
A
206
−14.118
−17.054
−29.521
1.00
30.16


ATOM
1612
N
GLY
A
207
−14.500
−17.145
−27.307
1.00
30.30


ATOM
1613
CA
GLY
A
207
−13.786
−18.419
−27.122
1.00
30.91


ATOM
1614
C
GLY
A
207
−12.294
−18.274
−27.375
1.00
31.55


ATOM
1615
O
GLY
A
207
−11.654
−19.173
−27.935
1.00
31.31


ATOM
1616
N
GLN
A
208
−11.746
−17.115
−26.989
1.00
31.08


ATOM
1617
CA
GLN
A
208
−10.311
−16.877
−27.031
1.00
31.10


ATOM
1618
CB
GLN
A
208
−9.999
−15.540
−27.703
1.00
31.08


ATOM
1619
CG
GLN
A
208
−10.451
−15.455
−29.142
1.00
33.86


ATOM
1620
CD
GLN
A
208
−9.469
−16.059
−30.126
1.00
38.19


ATOM
1621
OE1
GLN
A
208
−9.686
−15.999
−31.335
1.00
41.96


ATOM
1622
NE2
GLN
A
208
−8.386
−16.633
−29.626
1.00
38.96


ATOM
1623
C
GLN
A
208
−9.765
−16.909
−25.611
1.00
30.45


ATOM
1624
O
GLN
A
208
−10.516
−17.048
−24.658
1.00
30.63


ATOM
1625
N
SER
A
209
−8.451
−16.816
−25.469
1.00
29.96


ATOM
1626
CA
SER
A
209
−7.841
−16.898
−24.160
1.00
30.04


ATOM
1627
CB
SER
A
209
−6.382
−17.343
−24.297
1.00
30.04


ATOM
1628
OG
SER
A
209
−5.763
−17.371
−23.030
1.00
32.75


ATOM
1629
C
SER
A
209
−7.948
−15.564
−23.409
1.00
29.53


ATOM
1630
O
SER
A
209
−7.493
−14.532
−23.908
1.00
29.85


ATOM
1631
N
GLY
A
210
−8.545
−15.594
−22.216
1.00
28.41


ATOM
1632
CA
GLY
A
210
−8.745
−14.388
−21.401
1.00
27.16


ATOM
1633
C
GLY
A
210
−8.344
−14.480
−19.938
1.00
26.83


ATOM
1634
O
GLY
A
210
−8.425
−13.498
−19.203
1.00
26.61


ATOM
1635
N
SER
A
211
−7.888
−15.648
−19.497
1.00
25.85


ATOM
1636
CA
SER
A
211
−7.651
−15.867
−18.067
1.00
25.26


ATOM
1637
CB
SER
A
211
−7.401
−17.353
−17.783
1.00
25.87


ATOM
1638
OG
SER
A
211
−6.315
−17.789
−18.573
1.00
26.62


ATOM
1639
C
SER
A
211
−6.509
−15.026
−17.498
1.00
24.55


ATOM
1640
O
SER
A
211
−6.542
−14.676
−16.311
1.00
24.46


ATOM
1641
N
ALA
A
212
−5.505
−14.712
−18.323
1.00
23.56


ATOM
1642
CA
ALA
A
212
−4.423
−13.816
−17.906
1.00
23.42


ATOM
1643
CB
ALA
A
212
−3.417
−13.622
−19.031
1.00
23.66


ATOM
1644
C
ALA
A
212
−4.999
−12.450
−17.496
1.00
23.54


ATOM
1645
O
ALA
A
212
−4.566
−11.848
−16.513
1.00
24.00


ATOM
1646
N
TYR
A
213
−5.970
−11.979
−18.271
1.00
22.79


ATOM
1647
CA
TYR
A
213
−6.594
−10.676
−18.017
1.00
22.18


ATOM
1648
CB
TYR
A
213
−7.453
−10.241
−19.193
1.00
21.74


ATOM
1649
CG
TYR
A
213
−6.761
−10.345
−20.515
1.00
20.05


ATOM
1650
CD1
TYR
A
213
−7.461
−10.761
−21.637
1.00
20.58


ATOM
1651
CE1
TYR
A
213
−6.854
−10.854
−22.868
1.00
21.95


ATOM
1652
CZ
TYR
A
213
−5.503
−10.545
−22.988
1.00
20.62


ATOM
1653
OH
TYR
A
213
−4.930
−10.668
−24.220
1.00
21.72


ATOM
1654
CE2
TYR
A
213
−4.758
−10.149
−21.888
1.00
19.76


ATOM
1655
CD2
TYR
A
213
−5.400
−10.038
−20.647
1.00
20.61


ATOM
1656
C
TYR
A
213
−7.423
−10.710
−16.758
1.00
23.06


ATOM
1657
O
TYR
A
213
−7.320
−9.804
−15.939
1.00
22.56


ATOM
1658
N
SER
A
214
−8.226
−11.767
−16.578
1.00
23.15


ATOM
1659
CA
SER
A
214
−9.064
−11.832
−15.392
1.00
23.90


ATOM
1660
CB
SER
A
214
−10.244
−12.798
−15.580
1.00
24.54


ATOM
1661
OG
SER
A
214
−9.776
−14.085
−15.939
1.00
27.95


ATOM
1662
C
SER
A
214
−8.259
−12.122
−14.122
1.00
23.64


ATOM
1663
O
SER
A
214
−8.676
−11.762
−13.026
1.00
23.43


ATOM
1664
N
SER
A
215
−7.095
−12.743
−14.248
1.00
23.82


ATOM
1665
CA
SER
A
215
−6.295
−12.970
−13.050
1.00
24.66


ATOM
1666
CB
SER
A
215
−5.390
−14.205
−13.200
1.00
25.70


ATOM
1667
OG
SER
A
215
−4.267
−13.914
−14.004
1.00
29.15


ATOM
1668
C
SER
A
215
−5.491
−11.739
−12.610
1.00
23.98


ATOM
1669
O
SER
A
215
−5.217
−11.561
−11.421
1.00
24.09


ATOM
1670
N
VAL
A
216
−5.115
−10.894
−13.566
1.00
22.89


ATOM
1671
CA
VAL
A
216
−4.347
−9.679
−13.272
1.00
22.50


ATOM
1672
CB
VAL
A
216
−3.442
−9.296
−14.493
1.00
22.52


ATOM
1673
CG1
VAL
A
216
−2.855
−7.888
−14.369
1.00
24.11


ATOM
1674
CG2
VAL
A
216
−2.296
−10.317
−14.652
1.00
22.49


ATOM
1675
C
VAL
A
216
−5.256
−8.520
−12.801
1.00
21.88


ATOM
1676
O
VAL
A
216
−4.869
−7.745
−11.936
1.00
21.84


ATOM
1677
N
ALA
A
217
−6.475
−8.440
−13.332
1.00
21.86


ATOM
1678
CA
ALA
A
217
−7.374
−7.303
−13.050
1.00
21.59


ATOM
1679
CB
ALA
A
217
−8.721
−7.479
−13.760
1.00
21.26


ATOM
1680
C
ALA
A
217
−7.571
−6.968
−11.558
1.00
21.55


ATOM
1681
O
ALA
A
217
−7.447
−5.804
−11.165
1.00
21.20


ATOM
1682
N
PRO
A
218
−7.842
−7.988
−10.701
1.00
21.95


ATOM
1683
CA
PRO
A
218
−8.030
−7.700
−9.282
1.00
21.59


ATOM
1684
CB
PRO
A
218
−8.283
−9.104
−8.670
1.00
22.29


ATOM
1685
CG
PRO
A
218
−8.789
−9.905
−9.789
1.00
22.61


ATOM
1686
CD
PRO
A
218
−7.966
−9.435
−10.963
1.00
22.11


ATOM
1687
C
PRO
A
218
−6.798
−7.065
−8.634
1.00
21.27


ATOM
1688
O
PRO
A
218
−6.928
−6.299
−7.680
1.00
20.92


ATOM
1689
N
GLN
A
219
−5.608
−7.386
−9.141
1.00
21.17


ATOM
1690
CA
GLN
A
219
−4.378
−6.786
−8.609
1.00
21.51


ATOM
1691
CB
GLN
A
219
−3.149
−7.569
−9.084
1.00
22.72


ATOM
1692
CG
GLN
A
219
−3.113
−8.985
−8.516
1.00
24.90


ATOM
1693
CD
GLN
A
219
−3.323
−8.982
−7.015
1.00
29.57


ATOM
1694
OE1
GLN
A
219
−2.715
−8.188
−6.288
1.00
31.58


ATOM
1695
NE2
GLN
A
219
−4.207
−9.843
−6.545
1.00
33.22


ATOM
1696
C
GLN
A
219
−4.240
−5.301
−8.996
1.00
21.04


ATOM
1697
O
GLN
A
219
−3.687
−4.490
−8.229
1.00
21.07


ATOM
1698
N
VAL
A
220
−4.728
−4.973
−10.187
1.00
20.00


ATOM
1699
CA
VAL
A
220
−4.746
−3.577
−10.630
1.00
19.34


ATOM
1700
CB
VAL
A
220
−5.098
−3.456
−12.128
1.00
19.63


ATOM
1701
CG1
VAL
A
220
−4.991
−2.000
−12.581
1.00
19.15


ATOM
1702
CG2
VAL
A
220
−4.162
−4.342
−12.974
1.00
17.97


ATOM
1703
C
VAL
A
220
−5.730
−2.809
−9.737
1.00
19.65


ATOM
1704
O
VAL
A
220
−5.419
−1.728
−9.257
1.00
18.97


ATOM
1705
N
LEU
A
221
−6.903
−3.391
−9.490
1.00
20.12


ATOM
1706
CA
LEU
A
221
−7.895
−2.776
−8.620
1.00
20.83


ATOM
1707
CB
LEU
A
221
−9.180
−3.602
−8.599
1.00
20.48


ATOM
1708
CG
LEU
A
221
−10.336
−2.991
−7.790
1.00
22.48


ATOM
1709
CD1
LEU
A
221
−10.857
−1.726
−8.458
1.00
22.33


ATOM
1710
CD2
LEU
A
221
−11.430
−4.011
−7.637
1.00
22.51


ATOM
1711
C
LEU
A
221
−7.360
−2.591
−7.192
1.00
21.44


ATOM
1712
O
LEU
A
221
−7.617
−1.578
−6.539
1.00
20.45


ATOM
1713
N
CYS
A
222
−6.600
−3.572
−6.718
1.00
22.60


ATOM
1714
CA
CYS
A
222
−5.957
−3.477
−5.415
1.00
22.10


ATOM
1715
CB
CYS
A
222
−5.159
−4.749
−5.125
1.00
23.41


ATOM
1716
SG
CYS
A
222
−4.975
−5.000
−3.356
1.00
28.49


ATOM
1717
C
CYS
A
222
−5.035
−2.270
−5.317
1.00
21.22


ATOM
1718
O
CYS
A
222
−5.060
−1.531
−4.331
1.00
21.24


ATOM
1719
N
PHE
A
223
−4.210
−2.070
−6.347
1.00
20.11


ATOM
1720
CA
PHE
A
223
−3.287
−0.955
−6.368
1.00
19.03


ATOM
1721
CB
PHE
A
223
−2.334
−1.108
−7.558
1.00
19.15


ATOM
1722
CG
PHE
A
223
−1.297
−0.011
−7.669
1.00
19.23


ATOM
1723
CD1
PHE
A
223
−0.576
0.410
−6.558
1.00
19.90


ATOM
1724
CE1
PHE
A
223
0.380
1.417
−6.661
1.00
20.91


ATOM
1725
CZ
PHE
A
223
0.645
2.017
−7.902
1.00
21.07


ATOM
1726
CE2
PHE
A
223
−0.061
1.598
−9.024
1.00
18.81


ATOM
1727
CD2
PHE
A
223
−1.022
0.581
−8.909
1.00
18.23


ATOM
1728
C
PHE
A
223
−4.032
0.397
−6.423
1.00
18.38


ATOM
1729
O
PHE
A
223
−3.597
1.376
−5.818
1.00
18.27


ATOM
1730
N
LEU
A
224
−5.148
0.428
−7.142
1.00
18.45


ATOM
1731
CA
LEU
A
224
−5.957
1.665
−7.277
1.00
18.42


ATOM
1732
CB
LEU
A
224
−7.208
1.403
−8.127
1.00
17.70


ATOM
1733
CG
LEU
A
224
−7.990
2.645
−8.610
1.00
19.73


ATOM
1734
CD1
LEU
A
224
−7.133
3.427
−9.584
1.00
20.37


ATOM
1735
CD2
LEU
A
224
−9.302
2.228
−9.264
1.00
18.64


ATOM
1736
C
LEU
A
224
−6.385
2.226
−5.917
1.00
18.87


ATOM
1737
O
LEU
A
224
−6.553
3.438
−5.757
1.00
18.45


ATOM
1738
N
GLN
A
225
−6.578
1.336
−4.944
1.00
19.17


ATOM
1739
CA
GLN
A
225
−6.984
1.743
−3.585
1.00
20.00


ATOM
1740
CB
GLN
A
225
−7.340
0.511
−2.725
1.00
20.26


ATOM
1741
CG
GLN
A
225
−8.295
−0.463
−3.409
1.00
21.22


ATOM
1742
CD
GLN
A
225
−9.519
0.225
−3.993
1.00
22.53


ATOM
1743
OE1
GLN
A
225
−10.280
0.870
−3.262
1.00
23.09


ATOM
1744
NE2
GLN
A
225
−9.718
0.092
−5.302
1.00
19.33


ATOM
1745
C
GLN
A
225
−5.944
2.599
−2.871
1.00
20.19


ATOM
1746
O
GLN
A
225
−6.299
3.399
−2.009
1.00
20.64


ATOM
1747
N
ARG
A
226
−4.678
2.450
−3.253
1.00
20.51


ATOM
1748
CA
ARG
A
226
−3.564
3.144
−2.608
1.00
21.40


ATOM
1749
CB
ARG
A
226
−2.219
2.505
−2.990
1.00
22.72


ATOM
1750
CG
ARG
A
226
−2.081
1.010
−2.683
1.00
26.14


ATOM
1751
CD
ARG
A
226
−1.806
0.741
−1.204
1.00
32.16


ATOM
1752
NE
ARG
A
226
−3.035
0.843
−0.432
1.00
37.77


ATOM
1753
CZ
ARG
A
226
−3.997
−0.079
−0.413
1.00
41.09


ATOM
1754
NH1
ARG
A
226
−5.093
0.120
0.322
1.00
42.17


ATOM
1755
NH2
ARG
A
226
−3.874
−1.196
−1.127
1.00
42.78


ATOM
1756
C
ARG
A
226
−3.499
4.645
−2.915
1.00
21.23


ATOM
1757
O
ARG
A
226
−2.723
5.358
−2.288
1.00
20.95


ATOM
1758
N
PHE
A
227
−4.298
5.123
−3.869
1.00
20.28


ATOM
1759
CA
PHE
A
227
−4.280
6.545
−4.250
1.00
19.67


ATOM
1760
CB
PHE
A
227
−4.777
6.704
−5.693
1.00
19.40


ATOM
1761
CG
PHE
A
227
−3.814
6.195
−6.744
1.00
18.28


ATOM
1762
CD1
PHE
A
227
−3.733
4.831
−7.040
1.00
18.14


ATOM
1763
CE1
PHE
A
227
−2.855
4.355
−8.046
1.00
18.24


ATOM
1764
CZ
PHE
A
227
−2.034
5.264
−8.748
1.00
16.75


ATOM
1765
CE2
PHE
A
227
−2.113
6.641
−8.456
1.00
18.79


ATOM
1766
CD2
PHE
A
227
−3.005
7.091
−7.452
1.00
17.51


ATOM
1767
C
PHE
A
227
−5.126
7.435
−3.343
1.00
20.55


ATOM
1768
O
PHE
A
227
−4.967
8.659
−3.334
1.00
20.38


ATOM
1769
N
TRP
A
228
−6.032
6.820
−2.583
1.00
20.72


ATOM
1770
CA
TRP
A
228
−6.924
7.545
−1.671
1.00
20.71


ATOM
1771
CB
TRP
A
228
−8.036
6.596
−1.211
1.00
20.41


ATOM
1772
CG
TRP
A
228
−9.030
7.228
−0.283
1.00
20.59


ATOM
1773
CD1
TRP
A
228
−9.243
6.915
1.040
1.00
21.81


ATOM
1774
NE1
TRP
A
228
−10.255
7.722
1.557
1.00
22.69


ATOM
1775
CE2
TRP
A
228
−10.712
8.553
0.565
1.00
20.71


ATOM
1776
CD2
TRP
A
228
−9.958
8.280
−0.607
1.00
18.88


ATOM
1777
CE3
TRP
A
228
−10.225
9.014
−1.772
1.00
18.79


ATOM
1778
CZ3
TRP
A
228
−11.209
9.986
−1.734
1.00
20.13


ATOM
1779
CH2
TRP
A
228
−11.937
10.242
−0.552
1.00
21.18


ATOM
1780
CZ2
TRP
A
228
−11.710
9.537
0.601
1.00
21.65


ATOM
1781
C
TRP
A
228
−6.193
8.120
−0.463
1.00
21.38


ATOM
1782
O
TRP
A
228
−5.479
7.394
0.236
1.00
21.50


ATOM
1783
N
VAL
A
229
−6.379
9.416
−0.209
1.00
21.95


ATOM
1784
CA
VAL
A
229
−5.844
10.065
0.983
1.00
22.99


ATOM
1785
CB
VAL
A
229
−5.205
11.436
0.654
1.00
22.81


ATOM
1786
CG1
VAL
A
229
−4.490
12.026
1.871
1.00
23.48


ATOM
1787
CG2
VAL
A
229
−4.226
11.292
−0.493
1.00
23.28


ATOM
1788
C
VAL
A
229
−6.984
10.206
2.000
1.00
24.08


ATOM
1789
O
VAL
A
229
−7.803
11.119
1.899
1.00
23.70


ATOM
1790
N
SER
A
230
−7.044
9.298
2.974
1.00
25.37


ATOM
1791
CA
SER
A
230
−8.193
9.290
3.905
1.00
27.59


ATOM
1792
CB
SER
A
230
−8.254
8.000
4.728
1.00
27.67


ATOM
1793
OG
SER
A
230
−7.029
7.805
5.402
1.00
31.30


ATOM
1794
C
SER
A
230
−8.241
10.513
4.820
1.00
27.93


ATOM
1795
O
SER
A
230
−9.321
10.983
5.174
1.00
28.91


ATOM
1796
N
SER
A
231
−7.088
11.059
5.165
1.00
28.76


ATOM
1797
CA
SER
A
231
−7.059
12.237
6.030
1.00
29.72


ATOM
1798
CB
SER
A
231
−5.671
12.461
6.639
1.00
30.39


ATOM
1799
OG
SER
A
231
−4.703
12.713
5.635
1.00
34.43


ATOM
1800
C
SER
A
231
−7.566
13.491
5.323
1.00
29.39


ATOM
1801
O
SER
A
231
−8.154
14.364
5.966
1.00
30.97


ATOM
1802
N
GLY
A
232
−7.373
13.579
4.005
1.00
27.59


ATOM
1803
CA
GLY
A
232
−7.867
14.728
3.247
1.00
25.22


ATOM
1804
C
GLY
A
232
−9.181
14.518
2.493
1.00
23.25


ATOM
1805
O
GLY
A
232
−9.810
15.487
2.077
1.00
23.19


ATOM
1806
N
GLY
A
233
−9.589
13.265
2.320
1.00
20.97


ATOM
1807
CA
GLY
A
233
−10.809
12.937
1.578
1.00
19.35


ATOM
1808
C
GLY
A
233
−10.673
13.226
0.094
1.00
18.83


ATOM
1809
O
GLY
A
233
−11.636
13.655
−0.561
1.00
19.20


ATOM
1810
N
TYR
A
234
−9.487
12.977
−0.463
1.00
17.56


ATOM
1811
CA
TYR
A
234
−9.309
13.155
−1.915
1.00
17.17


ATOM
1812
CB
TYR
A
234
−8.851
14.584
−2.232
1.00
18.33


ATOM
1813
CG
TYR
A
234
−7.441
14.876
−1.758
1.00
20.39


ATOM
1814
CD1
TYR
A
234
−7.203
15.340
−0.454
1.00
20.72


ATOM
1815
CE1
TYR
A
234
−5.905
15.594
−0.018
1.00
24.11


ATOM
1816
CZ
TYR
A
234
−4.840
15.399
−0.897
1.00
23.78


ATOM
1817
OH
TYR
A
234
−3.556
15.663
−0.483
1.00
26.50


ATOM
1818
CE2
TYR
A
234
−5.055
14.956
−2.187
1.00
24.07


ATOM
1819
CD2
TYR
A
234
−6.353
14.699
−2.611
1.00
20.58


ATOM
1820
C
TYR
A
234
−8.318
12.141
−2.482
1.00
16.60


ATOM
1821
O
TYR
A
234
−7.615
11.465
−1.735
1.00
16.29


ATOM
1822
N
VAL
A
235
−8.260
12.059
−3.805
1.00
15.36


ATOM
1823
CA
VAL
A
235
−7.325
11.164
−4.472
1.00
15.62


ATOM
1824
CB
VAL
A
235
−7.948
10.638
−5.798
1.00
15.96


ATOM
1825
CG1
VAL
A
235
−6.889
9.893
−6.645
1.00
17.31


ATOM
1826
CG2
VAL
A
235
−9.134
9.723
−5.506
1.00
15.87


ATOM
1827
C
VAL
A
235
−6.011
11.904
−4.742
1.00
15.54


ATOM
1828
O
VAL
A
235
−6.006
12.998
−5.320
1.00
15.39


ATOM
1829
N
ASP
A
236
−4.886
11.316
−4.325
1.00
15.24


ATOM
1830
CA
ASP
A
236
−3.580
11.837
−4.705
1.00
15.22


ATOM
1831
CB
ASP
A
236
−2.533
11.431
−3.652
1.00
16.45


ATOM
1832
CG
ASP
A
236
−1.145
11.922
−3.970
1.00
18.62


ATOM
1833
OD1
ASP
A
236
−0.937
12.617
−4.992
1.00
17.17


ATOM
1834
OD2
ASP
A
236
−0.223
11.568
−3.182
1.00
22.79


ATOM
1835
C
ASP
A
236
−3.303
11.256
−6.098
1.00
15.06


ATOM
1836
O
ASP
A
236
−3.088
10.040
−6.261
1.00
15.39


ATOM
1837
N
SER
A
237
−3.384
12.104
−7.125
1.00
14.24


ATOM
1838
CA
SER
A
237
−3.518
11.587
−8.503
1.00
14.09


ATOM
1839
CB
SER
A
237
−4.000
12.697
−9.446
1.00
13.76


ATOM
1840
OG
SER
A
237
−5.312
13.094
−9.070
1.00
14.52


ATOM
1841
C
SER
A
237
−2.277
10.883
−9.053
1.00
14.22


ATOM
1842
O
SER
A
237
−2.376
10.067
−9.965
1.00
13.80


ATOM
1843
N
ASN
A
238
−1.099
11.219
−8.521
1.00
14.70


ATOM
1844
CA
ASN
A
238
0.116
10.547
−8.952
1.00
15.28


ATOM
1845
CB
ASN
A
238
0.968
11.439
−9.856
1.00
14.84


ATOM
1846
CG
ASN
A
238
0.277
11.742
−11.176
1.00
17.08


ATOM
1847
OD1
ASN
A
238
0.244
10.901
−12.072
1.00
16.61


ATOM
1848
ND2
ASN
A
238
−0.308
12.932
−11.278
1.00
16.63


ATOM
1849
C
ASN
A
238
0.912
10.150
−7.736
1.00
16.07


ATOM
1850
O
ASN
A
238
1.169
10.988
−6.890
1.00
15.88


ATOM
1851
N
ILE
A
239
1.280
8.875
−7.659
1.00
16.09


ATOM
1852
CA
ILE
A
239
2.125
8.410
−6.567
1.00
18.07


ATOM
1853
CB
ILE
A
239
1.340
7.452
−5.600
1.00
17.66


ATOM
1854
CG1
ILE
A
239
0.893
6.180
−6.336
1.00
18.85


ATOM
1855
CD1
ILE
A
239
0.184
5.109
−5.437
1.00
19.02


ATOM
1856
CG2
ILE
A
239
0.116
8.194
−4.974
1.00
16.96


ATOM
1857
C
ILE
A
239
3.381
7.760
−7.169
1.00
19.32


ATOM
1858
O
ILE
A
239
3.571
7.797
−8.392
1.00
19.19


ATOM
1859
N
ASN
A
240
4.242
7.170
−6.329
1.00
20.56


ATOM
1860
CA
ASN
A
240
5.517
6.617
−6.823
1.00
22.24


ATOM
1861
CB
ASN
A
240
5.275
5.385
−7.717
1.00
21.93


ATOM
1862
CG
ASN
A
240
4.874
4.153
−6.926
1.00
24.19


ATOM
1863
OD1
ASN
A
240
5.269
3.995
−5.772
1.00
25.98


ATOM
1864
ND2
ASN
A
240
4.083
3.278
−7.538
1.00
22.26


ATOM
1865
C
ASN
A
240
6.334
7.677
−7.571
1.00
23.27


ATOM
1866
O
ASN
A
240
7.000
7.381
−8.562
1.00
23.15


ATOM
1867
N
THR
A
241
6.261
8.919
−7.096
1.00
25.02


ATOM
1868
CA
THR
A
241
6.939
10.038
−7.729
1.00
28.12


ATOM
1869
CB
THR
A
241
6.044
10.720
−8.817
1.00
28.09


ATOM
1870
OG1
THR
A
241
6.741
11.836
−9.369
1.00
28.75


ATOM
1871
CG2
THR
A
241
4.727
11.208
−8.231
1.00
28.30


ATOM
1872
C
THR
A
241
7.302
11.065
−6.674
1.00
29.96


ATOM
1873
O
THR
A
241
6.749
11.037
−5.589
1.00
30.58


ATOM
1874
N
ASN
A
242
8.209
11.984
−6.991
1.00
33.17


ATOM
1875
CA
ASN
A
242
8.585
13.019
−6.024
1.00
36.07


ATOM
1876
CB
ASN
A
242
10.059
12.880
−5.616
1.00
37.13


ATOM
1877
CG
ASN
A
242
10.324
11.631
−4.771
1.00
40.96


ATOM
1878
OD1
ASN
A
242
9.509
11.235
−3.921
1.00
45.33


ATOM
1879
ND2
ASN
A
242
11.477
11.007
−4.998
1.00
44.43


ATOM
1880
C
ASN
A
242
8.321
14.427
−6.528
1.00
37.00


ATOM
1881
O
ASN
A
242
9.091
15.346
−6.245
1.00
37.94


ATOM
1882
N
GLU
A
243
7.210
14.602
−7.233
1.00
37.54


ATOM
1883
CA
GLU
A
243
6.895
15.869
−7.907
1.00
38.05


ATOM
1884
CB
GLU
A
243
5.775
15.638
−8.925
1.00
38.77


ATOM
1885
CG
GLU
A
243
5.650
16.732
−9.977
1.00
42.65


ATOM
1886
CD
GLU
A
243
6.959
16.985
−10.709
1.00
47.49


ATOM
1887
OE1
GLU
A
243
7.424
16.084
−11.453
1.00
49.14


ATOM
1888
OE2
GLU
A
243
7.520
18.090
−10.532
1.00
50.15


ATOM
1889
C
GLU
A
243
6.559
17.088
−7.015
1.00
37.15


ATOM
1890
O
GLU
A
243
6.645
18.240
−7.469
1.00
38.39


ATOM
1891
N
GLY
A
244
6.174
16.873
−5.766
1.00
35.69


ATOM
1892
CA
GLY
A
244
5.858
18.019
−4.911
1.00
33.51


ATOM
1893
C
GLY
A
244
4.609
18.775
−5.369
1.00
31.80


ATOM
1894
O
GLY
A
244
4.634
19.999
−5.535
1.00
33.32


ATOM
1895
N
ARG
A
245
3.529
18.036
−5.612
1.00
27.92


ATOM
1896
CA
ARG
A
245
2.200
18.618
−5.781
1.00
24.21


ATOM
1897
CB
ARG
A
245
1.638
18.224
−7.130
1.00
24.30


ATOM
1898
CG
ARG
A
245
2.410
18.842
−8.275
1.00
24.62


ATOM
1899
CD
ARG
A
245
1.625
18.681
−9.532
1.00
22.11


ATOM
1900
NE
ARG
A
245
2.462
18.829
−10.713
1.00
21.13


ATOM
1901
CZ
ARG
A
245
2.114
18.302
−11.878
1.00
21.50


ATOM
1902
NH1
ARG
A
245
0.982
17.621
−11.945
1.00
18.57


ATOM
1903
NH2
ARG
A
245
2.883
18.443
−12.951
1.00
20.83


ATOM
1904
C
ARG
A
245
1.295
18.040
−4.718
1.00
21.84


ATOM
1905
O
ARG
A
245
1.624
17.021
−4.128
1.00
20.65


ATOM
1906
N
THR
A
246
0.140
18.652
−4.483
1.00
19.15


ATOM
1907
CA
THR
A
246
−0.824
18.058
−3.540
1.00
17.46


ATOM
1908
CB
THR
A
246
−1.989
18.997
−3.238
1.00
17.87


ATOM
1909
OG1
THR
A
246
−2.752
19.155
−4.440
1.00
15.85


ATOM
1910
CG2
THR
A
246
−1.495
20.370
−2.730
1.00
17.50


ATOM
1911
C
THR
A
246
−1.426
16.769
−4.103
1.00
17.25


ATOM
1912
O
THR
A
246
−1.884
15.914
−3.351
1.00
17.57


ATOM
1913
N
GLY
A
247
−1.482
16.646
−5.430
1.00
15.60


ATOM
1914
CA
GLY
A
247
−2.148
15.492
−6.054
1.00
15.02


ATOM
1915
C
GLY
A
247
−3.609
15.761
−6.396
1.00
14.69


ATOM
1916
O
GLY
A
247
−4.260
14.939
−7.059
1.00
14.45


ATOM
1917
N
LYS
A
248
−4.137
16.890
−5.928
1.00
13.43


ATOM
1918
CA
LYS
A
248
−5.508
17.286
−6.259
1.00
13.00


ATOM
1919
CB
LYS
A
248
−5.969
18.453
−5.396
1.00
12.32


ATOM
1920
CG
LYS
A
248
−5.965
18.179
−3.881
1.00
13.12


ATOM
1921
CD
LYS
A
248
−6.133
19.493
−3.102
1.00
14.08


ATOM
1922
CE
LYS
A
248
−5.985
19.253
−1.584
1.00
17.84


ATOM
1923
NZ
LYS
A
248
−6.335
20.492
−0.835
1.00
16.74


ATOM
1924
C
LYS
A
248
−5.490
17.713
−7.736
1.00
12.73


ATOM
1925
O
LYS
A
248
−4.866
18.707
−8.104
1.00
12.75


ATOM
1926
N
ASP
A
249
−6.185
16.964
−8.580
1.00
11.92


ATOM
1927
CA
ASP
A
249
−5.958
17.098
−10.024
1.00
11.16


ATOM
1928
CB
ASP
A
249
−4.761
16.199
−10.385
1.00
10.83


ATOM
1929
CG
ASP
A
249
−4.268
16.349
−11.831
1.00
12.54


ATOM
1930
OD1
ASP
A
249
−5.078
16.422
−12.785
1.00
11.42


ATOM
1931
OD2
ASP
A
249
−3.025
16.342
−12.001
1.00
13.30


ATOM
1932
C
ASP
A
249
−7.232
16.577
−10.662
1.00
11.38


ATOM
1933
O
ASP
A
249
−7.774
15.542
−10.236
1.00
10.86


ATOM
1934
N
VAL
A
250
−7.700
17.265
−11.703
1.00
11.28


ATOM
1935
CA
VAL
A
250
−8.885
16.793
−12.438
1.00
11.59


ATOM
1936
CB
VAL
A
250
−9.366
17.859
−13.493
1.00
12.49


ATOM
1937
CG1
VAL
A
250
−8.480
17.815
−14.728
1.00
13.03


ATOM
1938
CG2
VAL
A
250
−10.859
17.654
−13.852
1.00
13.75


ATOM
1939
C
VAL
A
250
−8.711
15.386
−13.064
1.00
11.77


ATOM
1940
O
VAL
A
250
−9.698
14.750
−13.467
1.00
11.71


ATOM
1941
N
ASN
A
251
−7.461
14.925
−13.168
1.00
10.73


ATOM
1942
CA
ASN
A
251
−7.131
13.491
−13.378
1.00
11.20


ATOM
1943
CB
ASN
A
251
−5.699
13.265
−12.813
1.00
10.94


ATOM
1944
CG
ASN
A
251
−5.221
11.810
−12.892
1.00
11.58


ATOM
1945
OD1
ASN
A
251
−5.986
10.864
−12.672
1.00
12.47


ATOM
1946
ND2
ASN
A
251
−3.898
11.639
−13.164
1.00
14.40


ATOM
1947
C
ASN
A
251
−8.151
12.560
−12.706
1.00
10.99


ATOM
1948
O
ASN
A
251
−8.755
11.706
−13.355
1.00
11.49


ATOM
1949
N
SER
A
252
−8.407
12.774
−11.417
1.00
11.45


ATOM
1950
CA
SER
A
252
−9.293
11.876
−10.634
1.00
11.79


ATOM
1951
CB
SER
A
252
−9.062
12.155
−9.149
1.00
13.31


ATOM
1952
OG
SER
A
252
−9.338
13.524
−8.882
1.00
13.41


ATOM
1953
C
SER
A
252
−10.784
12.002
−10.996
1.00
11.39


ATOM
1954
O
SER
A
252
−11.532
11.023
−10.964
1.00
12.69


ATOM
1955
N
VAL
A
253
−11.199
13.203
−11.383
1.00
10.56


ATOM
1956
CA
VAL
A
253
−12.582
13.459
−11.821
1.00
10.70


ATOM
1957
CB
VAL
A
253
−12.884
15.004
−11.856
1.00
11.02


ATOM
1958
CG1
VAL
A
253
−14.335
15.262
−12.345
1.00
11.24


ATOM
1959
CG2
VAL
A
253
−12.711
15.585
−10.449
1.00
10.91


ATOM
1960
C
VAL
A
253
−12.810
12.827
−13.187
1.00
11.38


ATOM
1961
O
VAL
A
253
−13.824
12.143
−13.407
1.00
11.69


ATOM
1962
N
LEU
A
254
−11.866
13.059
−14.108
1.00
11.32


ATOM
1963
CA
LEU
A
254
−11.891
12.393
−15.417
1.00
12.12


ATOM
1964
CB
LEU
A
254
−10.635
12.759
−16.238
1.00
11.95


ATOM
1965
CG
LEU
A
254
−10.634
14.202
−16.763
1.00
12.23


ATOM
1966
CD1
LEU
A
254
−9.266
14.564
−17.330
1.00
12.77


ATOM
1967
CD2
LEU
A
254
−11.714
14.371
−17.845
1.00
15.26


ATOM
1968
C
LEU
A
254
−11.963
10.872
−15.271
1.00
12.22


ATOM
1969
O
LEU
A
254
−12.675
10.201
−16.024
1.00
12.01


ATOM
1970
N
THR
A
255
−11.208
10.338
−14.315
1.00
11.58


ATOM
1971
CA
THR
A
255
−11.219
8.913
−14.042
1.00
12.53


ATOM
1972
CB
THR
A
255
−10.267
8.552
−12.890
1.00
12.83


ATOM
1973
OG1
THR
A
255
−8.935
8.933
−13.240
1.00
13.00


ATOM
1974
CG2
THR
A
255
−10.300
7.035
−12.634
1.00
15.06


ATOM
1975
C
THR
A
255
−12.632
8.448
−13.705
1.00
13.12


ATOM
1976
O
THR
A
255
−13.131
7.467
−14.285
1.00
13.49


ATOM
1977
N
SER
A
256
−13.289
9.158
−12.790
1.00
13.46


ATOM
1978
CA
SER
A
256
−14.641
8.781
−12.343
1.00
12.85


ATOM
1979
CB
SER
A
256
−15.152
9.760
−11.282
1.00
13.40


ATOM
1980
OG
SER
A
256
−16.332
9.252
−10.674
1.00
16.69


ATOM
1981
C
SER
A
256
−15.610
8.705
−13.518
1.00
13.13


ATOM
1982
O
SER
A
256
−16.360
7.711
−13.654
1.00
13.10


ATOM
1983
N
ILE
A
257
−15.594
9.728
−14.377
1.00
12.32


ATOM
1984
CA
ILE
A
257
−16.523
9.784
−15.513
1.00
12.32


ATOM
1985
CB
ILE
A
257
−16.747
11.215
−16.072
1.00
11.55


ATOM
1986
CG1
ILE
A
257
−15.482
11.773
−16.764
1.00
11.38


ATOM
1987
CD1
ILE
A
257
−15.699
13.143
−17.441
1.00
13.23


ATOM
1988
CG2
ILE
A
257
−17.257
12.166
−14.942
1.00
13.70


ATOM
1989
C
ILE
A
257
−16.220
8.795
−16.653
1.00
12.79


ATOM
1990
O
ILE
A
257
−17.150
8.319
−17.338
1.00
13.25


ATOM
1991
N
HIS
A
258
−14.941
8.487
−16.855
1.00
12.71


ATOM
1992
CA
HIS
A
258
−14.565
7.566
−17.931
1.00
13.41


ATOM
1993
CB
HIS
A
258
−13.194
7.947
−18.498
1.00
12.06


ATOM
1994
CG
HIS
A
258
−13.268
9.175
−19.341
1.00
13.92


ATOM
1995
ND1
HIS
A
258
−13.942
9.196
−20.547
1.00
16.01


ATOM
1996
CE1
HIS
A
258
−13.891
10.421
−21.047
1.00
18.57


ATOM
1997
NE2
HIS
A
258
−13.256
11.199
−20.189
1.00
14.08


ATOM
1998
CD2
HIS
A
258
−12.861
10.449
−19.108
1.00
13.51


ATOM
1999
C
HIS
A
258
−14.649
6.091
−17.565
1.00
14.12


ATOM
2000
O
HIS
A
258
−14.645
5.239
−18.454
1.00
14.90


ATOM
2001
N
THR
A
259
−14.752
5.801
−16.274
1.00
13.93


ATOM
2002
CA
THR
A
259
−15.034
4.420
−15.807
1.00
14.91


ATOM
2003
CB
THR
A
259
−13.933
3.856
−14.899
1.00
14.46


ATOM
2004
OG1
THR
A
259
−13.788
4.647
−13.705
1.00
15.66


ATOM
2005
CG2
THR
A
259
−12.589
3.802
−15.677
1.00
15.81


ATOM
2006
C
THR
A
259
−16.433
4.248
−15.173
1.00
14.21


ATOM
2007
O
THR
A
259
−16.709
3.235
−14.546
1.00
14.95


ATOM
2008
N
PHE
A
260
−17.290
5.238
−15.367
1.00
14.76


ATOM
2009
CA
PHE
A
260
−18.691
5.194
−14.926
1.00
15.13


ATOM
2010
CB
PHE
A
260
−19.377
6.492
−15.379
1.00
15.81


ATOM
2011
CG
PHE
A
260
−20.886
6.508
−15.228
1.00
15.47


ATOM
2012
CD1
PHE
A
260
−21.505
6.188
−14.015
1.00
17.59


ATOM
2013
CE1
PHE
A
260
−22.903
6.259
−13.898
1.00
19.11


ATOM
2014
CZ
PHE
A
260
−23.682
6.653
−14.991
1.00
17.18


ATOM
2015
CE2
PHE
A
260
−23.082
6.994
−16.178
1.00
18.04


ATOM
2016
CD2
PHE
A
260
−21.679
6.917
−16.296
1.00
17.22


ATOM
2017
C
PHE
A
260
−19.436
3.977
−15.475
1.00
15.55


ATOM
2018
O
PHE
A
260
−19.426
3.725
−16.684
1.00
15.81


ATOM
2019
N
ASP
A
261
−20.093
3.235
−14.586
1.00
15.51


ATOM
2020
CA
ASP
A
261
−21.008
2.176
−15.006
1.00
16.05


ATOM
2021
CB
ASP
A
261
−20.303
0.813
−15.015
1.00
16.46


ATOM
2022
CG
ASP
A
261
−21.205
−0.321
−15.490
1.00
17.60


ATOM
2023
OD1
ASP
A
261
−22.440
−0.122
−15.579
1.00
18.97


ATOM
2024
OD2
ASP
A
261
−20.656
−1.404
−15.810
1.00
18.29


ATOM
2025
C
ASP
A
261
−22.117
2.185
−13.972
1.00
16.30


ATOM
2026
O
ASP
A
261
−21.882
1.809
−12.840
1.00
15.53


ATOM
2027
N
PRO
A
262
−23.320
2.610
−14.374
1.00
18.21


ATOM
2028
CA
PRO
A
262
−24.438
2.716
−13.412
1.00
20.39


ATOM
2029
CB
PRO
A
262
−25.589
3.308
−14.247
1.00
20.43


ATOM
2030
CG
PRO
A
262
−25.235
3.044
−15.669
1.00
20.06


ATOM
2031
CD
PRO
A
262
−23.709
2.994
−15.734
1.00
17.40


ATOM
2032
C
PRO
A
262
−24.815
1.382
−12.753
1.00
22.31


ATOM
2033
O
PRO
A
262
−25.356
1.374
−11.622
1.00
22.24


ATOM
2034
N
ASN
A
263
−24.508
0.267
−13.421
1.00
22.99


ATOM
2035
CA
ASN
A
263
−24.750
−1.048
−12.838
1.00
25.42


ATOM
2036
CB
ASN
A
263
−24.574
−2.149
−13.890
1.00
26.62


ATOM
2037
CG
ASN
A
263
−25.680
−2.128
−14.948
1.00
30.74


ATOM
2038
OD1
ASN
A
263
−26.688
−1.419
−14.814
1.00
35.92


ATOM
2039
ND2
ASN
A
263
−25.490
−2.906
−16.007
1.00
35.87


ATOM
2040
C
ASN
A
263
−23.894
−1.316
−11.598
1.00
25.45


ATOM
2041
O
ASN
A
263
−24.210
−2.190
−10.795
1.00
26.56


ATOM
2042
N
LEU
A
264
−22.835
−0.529
−11.413
1.00
24.54


ATOM
2043
CA
LEU
A
264
−22.022
−0.616
−10.213
1.00
24.27


ATOM
2044
CB
LEU
A
264
−20.549
−0.287
−10.520
1.00
24.43


ATOM
2045
CG
LEU
A
264
−19.752
−1.346
−11.288
1.00
25.38


ATOM
2046
CD1
LEU
A
264
−18.375
−0.809
−11.659
1.00
26.05


ATOM
2047
CD2
LEU
A
264
−19.619
−2.672
−10.523
1.00
26.24


ATOM
2048
C
LEU
A
264
−22.542
0.273
−9.066
1.00
23.47


ATOM
2049
O
LEU
A
264
−21.956
0.292
−7.988
1.00
23.97


ATOM
2050
N
GLY
A
265
−23.631
1.000
−9.292
1.00
23.32


ATOM
2051
CA
GLY
A
265
−24.218
1.840
−8.237
1.00
22.74


ATOM
2052
C
GLY
A
265
−23.204
2.843
−7.729
1.00
21.84


ATOM
2053
O
GLY
A
265
−22.416
3.373
−8.510
1.00
22.83


ATOM
2054
N
CYS
A
266
−23.175
3.086
−6.424
1.00
21.37


ATOM
2055
CA
CYS
A
266
−22.233
4.073
−5.883
1.00
21.00


ATOM
2056
CB
CYS
A
266
−22.947
5.049
−4.936
1.00
20.86


ATOM
2057
SG
CYS
A
266
−24.347
5.912
−5.711
1.00
20.96


ATOM
2058
C
CYS
A
266
−20.992
3.427
−5.275
1.00
20.98


ATOM
2059
O
CYS
A
266
−20.513
3.814
−4.203
1.00
21.01


ATOM
2060
N
ASP
A
267
−20.462
2.443
−6.002
1.00
20.39


ATOM
2061
CA
ASP
A
267
−19.303
1.686
−5.577
1.00
20.67


ATOM
2062
CB
ASP
A
267
−18.961
0.621
−6.618
1.00
20.84


ATOM
2063
CG
ASP
A
267
−17.666
−0.101
−6.288
1.00
24.28


ATOM
2064
OD1
ASP
A
267
−16.852
−0.322
−7.200
1.00
25.84


ATOM
2065
OD2
ASP
A
267
−17.455
−0.407
−5.098
1.00
27.46


ATOM
2066
C
ASP
A
267
−18.072
2.567
−5.391
1.00
19.78


ATOM
2067
O
ASP
A
267
−17.593
3.161
−6.353
1.00
18.61


ATOM
2068
N
ALA
A
268
−17.544
2.621
−4.174
1.00
18.35


ATOM
2069
CA
ALA
A
268
−16.315
3.395
−3.944
1.00
19.35


ATOM
2070
CB
ALA
A
268
−16.207
3.868
−2.472
1.00
19.46


ATOM
2071
C
ALA
A
268
−15.017
2.701
−4.415
1.00
19.46


ATOM
2072
O
ALA
A
268
−14.009
3.371
−4.665
1.00
19.42


ATOM
2073
N
GLY
A
269
−15.029
1.370
−4.534
1.00
19.01


ATOM
2074
CA
GLY
A
269
−13.826
0.644
−4.936
1.00
19.17


ATOM
2075
C
GLY
A
269
−13.370
1.032
−6.343
1.00
18.75


ATOM
2076
O
GLY
A
269
−12.175
1.134
−6.624
1.00
19.54


ATOM
2077
N
THR
A
270
−14.330
1.257
−7.230
1.00
17.92


ATOM
2078
CA
THR
A
270
−14.016
1.662
−8.594
1.00
18.35


ATOM
2079
CB
THR
A
270
−14.852
0.882
−9.616
1.00
18.43


ATOM
2080
OG1
THR
A
270
−16.246
1.085
−9.350
1.00
18.51


ATOM
2081
CG2
THR
A
270
−14.529
−0.626
−9.555
1.00
19.75


ATOM
2082
C
THR
A
270
−14.261
3.172
−8.771
1.00
18.25


ATOM
2083
O
THR
A
270
−14.326
3.674
−9.904
1.00
17.88


ATOM
2084
N
PHE
A
271
−14.434
3.880
−7.650
1.00
17.17


ATOM
2085
CA
PHE
A
271
−14.531
5.359
−7.656
1.00
17.38


ATOM
2086
CB
PHE
A
271
−13.183
5.965
−8.121
1.00
17.67


ATOM
2087
CG
PHE
A
271
−12.946
7.376
−7.673
1.00
21.97


ATOM
2088
CD1
PHE
A
271
−12.656
7.653
−6.337
1.00
24.90


ATOM
2089
CE1
PHE
A
271
−12.447
8.981
−5.923
1.00
24.46


ATOM
2090
CZ
PHE
A
271
−12.474
10.043
−6.863
1.00
23.20


ATOM
2091
CE2
PHE
A
271
−12.733
9.783
−8.196
1.00
21.92


ATOM
2092
CD2
PHE
A
271
−12.956
8.436
−8.599
1.00
24.30


ATOM
2093
C
PHE
A
271
−15.677
5.856
−8.551
1.00
16.63


ATOM
2094
O
PHE
A
271
−15.479
6.764
−9.358
1.00
15.93


ATOM
2095
N
GLN
A
272
−16.861
5.249
−8.439
1.00
15.21


ATOM
2096
CA
GLN
A
272
−18.011
5.673
−9.251
1.00
14.97


ATOM
2097
CB
GLN
A
272
−19.227
4.755
−9.013
1.00
14.93


ATOM
2098
CG
GLN
A
272
−19.021
3.355
−9.615
1.00
16.30


ATOM
2099
CD
GLN
A
272
−18.755
3.413
−11.102
1.00
15.81


ATOM
2100
OE1
GLN
A
272
−19.575
3.909
−11.883
1.00
16.97


ATOM
2101
NE2
GLN
A
272
−17.617
2.861
−11.512
1.00
18.50


ATOM
2102
C
GLN
A
272
−18.402
7.118
−8.929
1.00
14.73


ATOM
2103
O
GLN
A
272
−18.194
7.555
−7.800
1.00
15.60


ATOM
2104
N
PRO
A
273
−18.955
7.859
−9.914
1.00
14.45


ATOM
2105
CA
PRO
A
273
−19.342
9.255
−9.682
1.00
14.57


ATOM
2106
CB
PRO
A
273
−20.157
9.597
−10.927
1.00
14.64


ATOM
2107
CG
PRO
A
273
−19.443
8.767
−12.031
1.00
14.70


ATOM
2108
CD
PRO
A
273
−19.156
7.458
−11.326
1.00
14.10


ATOM
2109
C
PRO
A
273
−20.162
9.542
−8.407
1.00
15.22


ATOM
2110
O
PRO
A
273
−19.910
10.562
−7.752
1.00
15.03


ATOM
2111
N
CYS
A
274
−21.130
8.682
−8.075
1.00
15.76


ATOM
2112
CA
CYS
A
274
−21.926
8.913
−6.853
1.00
16.22


ATOM
2113
CB
CYS
A
274
−23.389
8.489
−7.039
1.00
16.57


ATOM
2114
SG
CYS
A
274
−23.611
6.769
−7.423
1.00
17.39


ATOM
2115
C
CYS
A
274
−21.331
8.281
−5.605
1.00
16.64


ATOM
2116
O
CYS
A
274
−21.958
8.329
−4.529
1.00
16.55


ATOM
2117
N
SER
A
275
−20.137
7.681
−5.715
1.00
15.61


ATOM
2118
CA
SER
A
275
−19.476
7.117
−4.528
1.00
15.81


ATOM
2119
CB
SER
A
275
−18.244
6.253
−4.877
1.00
15.06


ATOM
2120
OG
SER
A
275
−17.144
7.041
−5.315
1.00
14.92


ATOM
2121
C
SER
A
275
−19.097
8.232
−3.545
1.00
16.06


ATOM
2122
O
SER
A
275
−18.818
9.366
−3.949
1.00
14.39


ATOM
2123
N
ASP
A
276
−19.103
7.919
−2.248
1.00
16.42


ATOM
2124
CA
ASP
A
276
−18.731
8.935
−1.271
1.00
16.52


ATOM
2125
CB
ASP
A
276
−19.020
8.511
0.189
1.00
16.27


ATOM
2126
CG
ASP
A
276
−18.244
7.281
0.656
1.00
19.14


ATOM
2127
OD1
ASP
A
276
−18.371
7.001
1.873
1.00
19.62


ATOM
2128
OD2
ASP
A
276
−17.544
6.593
−0.120
1.00
17.10


ATOM
2129
C
ASP
A
276
−17.312
9.469
−1.492
1.00
16.17


ATOM
2130
O
ASP
A
276
−17.084
10.683
−1.415
1.00
15.20


ATOM
2131
N
LYS
A
277
−16.381
8.577
−1.823
1.00
15.43


ATOM
2132
CA
LYS
A
277
−14.994
8.982
−2.115
1.00
15.34


ATOM
2133
CB
LYS
A
277
−14.089
7.763
−2.326
1.00
15.23


ATOM
2134
CG
LYS
A
277
−13.924
6.905
−1.059
1.00
17.01


ATOM
2135
CD
LYS
A
277
−12.752
5.929
−1.204
1.00
21.20


ATOM
2136
CE
LYS
A
277
−12.662
5.017
0.015
1.00
22.94


ATOM
2137
NZ
LYS
A
277
−11.533
4.067
−0.165
1.00
29.19


ATOM
2138
C
LYS
A
277
−14.900
9.915
−3.324
1.00
14.30


ATOM
2139
O
LYS
A
277
−14.152
10.887
−3.288
1.00
14.70


ATOM
2140
N
ALA
A
278
−15.644
9.620
−4.393
1.00
14.45


ATOM
2141
CA
ALA
A
278
−15.588
10.464
−5.605
1.00
13.61


ATOM
2142
CB
ALA
A
278
−16.250
9.775
−6.783
1.00
13.30


ATOM
2143
C
ALA
A
278
−16.210
11.827
−5.357
1.00
13.50


ATOM
2144
O
ALA
A
278
−15.730
12.840
−5.864
1.00
13.02


ATOM
2145
N
LEU
A
279
−17.283
11.855
−4.565
1.00
13.22


ATOM
2146
CA
LEU
A
279
−17.936
13.132
−4.239
1.00
12.92


ATOM
2147
CB
LEU
A
279
−19.323
12.893
−3.625
1.00
13.21


ATOM
2148
CG
LEU
A
279
−20.384
12.358
−4.601
1.00
13.94


ATOM
2149
CD1
LEU
A
279
−21.707
11.969
−3.887
1.00
17.68


ATOM
2150
CD2
LEU
A
279
−20.653
13.319
−5.781
1.00
17.52


ATOM
2151
C
LEU
A
279
−17.065
13.995
−3.348
1.00
12.84


ATOM
2152
O
LEU
A
279
−16.941
15.203
−3.577
1.00
13.54


ATOM
2153
N
SER
A
280
−16.463
13.390
−2.315
1.00
12.45


ATOM
2154
CA
SER
A
280
−15.502
14.106
−1.459
1.00
13.53


ATOM
2155
CB
SER
A
280
−14.951
13.168
−0.364
1.00
13.65


ATOM
2156
OG
SER
A
280
−14.008
13.863
0.468
1.00
15.07


ATOM
2157
C
SER
A
280
−14.332
14.672
−2.285
1.00
14.09


ATOM
2158
O
SER
A
280
−13.925
15.856
−2.130
1.00
13.43


ATOM
2159
N
ASN
A
281
−13.795
13.830
−3.166
1.00
13.27


ATOM
2160
CA
ASN
A
281
−12.690
14.257
−4.027
1.00
13.19


ATOM
2161
CB
ASN
A
281
−12.239
13.078
−4.888
1.00
12.05


ATOM
2162
CG
ASN
A
281
−11.116
13.455
−5.849
1.00
13.13


ATOM
2163
OD1
ASN
A
281
−9.989
13.637
−5.446
1.00
13.26


ATOM
2164
ND2
ASN
A
281
−11.442
13.573
−7.124
1.00
11.63


ATOM
2165
C
ASN
A
281
−13.096
15.432
−4.933
1.00
12.33


ATOM
2166
O
ASN
A
281
−12.330
16.380
−5.109
1.00
13.49


ATOM
2167
N
LEU
A
282
−14.287
15.355
−5.506
1.00
12.05


ATOM
2168
CA
LEU
A
282
−14.760
16.422
−6.376
1.00
13.21


ATOM
2169
CB
LEU
A
282
−16.147
16.109
−6.949
1.00
12.17


ATOM
2170
CG
LEU
A
282
−16.791
17.216
−7.820
1.00
14.57


ATOM
2171
CD1
LEU
A
282
−16.011
17.378
−9.126
1.00
16.58


ATOM
2172
CD2
LEU
A
282
−18.241
16.863
−8.170
1.00
15.68


ATOM
2173
C
LEU
A
282
−14.739
17.754
−5.638
1.00
12.69


ATOM
2174
O
LEU
A
282
−14.201
18.735
−6.153
1.00
13.45


ATOM
2175
N
LYS
A
283
−15.283
17.791
−4.415
1.00
12.75


ATOM
2176
CA
LYS
A
283
−15.306
19.026
−3.656
1.00
12.89


ATOM
2177
CB
LYS
A
283
−16.079
18.860
−2.334
1.00
12.90


ATOM
2178
CG
LYS
A
283
−15.912
20.089
−1.432
1.00
13.94


ATOM
2179
CD
LYS
A
283
−16.909
20.076
−0.252
1.00
14.67


ATOM
2180
CE
LYS
A
283
−16.530
21.136
0.797
1.00
13.67


ATOM
2181
NZ
LYS
A
283
−16.315
22.489
0.212
1.00
19.03


ATOM
2182
C
LYS
A
283
−13.889
19.537
−3.385
1.00
12.43


ATOM
2183
O
LYS
A
283
−13.612
20.710
−3.556
1.00
12.14


ATOM
2184
N
VAL
A
284
−12.988
18.652
−2.966
1.00
12.02


ATOM
2185
CA
VAL
A
284
−11.624
19.055
−2.633
1.00
12.77


ATOM
2186
CB
VAL
A
284
−10.845
17.875
−2.014
1.00
13.17


ATOM
2187
CG1
VAL
A
284
−9.320
18.169
−1.936
1.00
13.21


ATOM
2188
CG2
VAL
A
284
−11.391
17.557
−0.630
1.00
15.81


ATOM
2189
C
VAL
A
284
−10.927
19.599
−3.881
1.00
12.74


ATOM
2190
O
VAL
A
284
−10.228
20.636
−3.827
1.00
12.21


ATOM
2191
N
VAL
A
285
−11.153
18.927
−5.012
1.00
11.54


ATOM
2192
CA
VAL
A
285
−10.560
19.389
−6.287
1.00
12.35


ATOM
2193
CB
VAL
A
285
−10.694
18.330
−7.425
1.00
12.36


ATOM
2194
CG1
VAL
A
285
−10.316
18.944
−8.813
1.00
12.25


ATOM
2195
CG2
VAL
A
285
−9.795
17.104
−7.140
1.00
13.25


ATOM
2196
C
VAL
A
285
−11.130
20.770
−6.712
1.00
12.08


ATOM
2197
O
VAL
A
285
−10.367
21.696
−6.989
1.00
12.60


ATOM
2198
N
VAL
A
286
−12.452
20.913
−6.728
1.00
11.87


ATOM
2199
CA
VAL
A
286
−13.089
22.196
−7.074
1.00
12.87


ATOM
2200
CB
VAL
A
286
−14.631
22.080
−7.038
1.00
13.01


ATOM
2201
CG1
VAL
A
286
−15.300
23.468
−7.140
1.00
14.31


ATOM
2202
CG2
VAL
A
286
−15.103
21.157
−8.200
1.00
14.42


ATOM
2203
C
VAL
A
286
−12.586
23.324
−6.164
1.00
12.84


ATOM
2204
O
VAL
A
286
−12.206
24.402
−6.635
1.00
13.75


ATOM
2205
N
ASP
A
287
−12.552
23.064
−4.853
1.00
12.85


ATOM
2206
CA
ASP
A
287
−12.116
24.059
−3.870
1.00
13.90


ATOM
2207
CB
ASP
A
287
−12.199
23.506
−2.440
1.00
13.12


ATOM
2208
CG
ASP
A
287
−13.637
23.441
−1.924
1.00
16.00


ATOM
2209
OD1
ASP
A
287
−14.541
24.002
−2.583
1.00
16.20


ATOM
2210
OD2
ASP
A
287
−13.857
22.835
−0.858
1.00
16.76


ATOM
2211
C
ASP
A
287
−10.727
24.564
−4.136
1.00
14.28


ATOM
2212
O
ASP
A
287
−10.425
25.722
−3.841
1.00
15.53


ATOM
2213
N
SER
A
288
−9.862
23.709
−4.677
1.00
14.50


ATOM
2214
CA
SER
A
288
−8.478
24.093
−4.949
1.00
14.58


ATOM
2215
CB
SER
A
288
−7.625
22.843
−5.229
1.00
14.15


ATOM
2216
OG
SER
A
288
−7.758
22.417
−6.565
1.00
13.73


ATOM
2217
C
SER
A
288
−8.326
25.186
−6.038
1.00
14.61


ATOM
2218
O
SER
A
288
−7.274
25.847
−6.143
1.00
14.59


ATOM
2219
N
PHE
A
289
−9.392
25.416
−6.809
1.00
13.99


ATOM
2220
CA
PHE
A
289
−9.419
26.447
−7.831
1.00
14.09


ATOM
2221
CB
PHE
A
289
−9.994
25.882
−9.135
1.00
13.52


ATOM
2222
CG
PHE
A
289
−9.169
24.807
−9.704
1.00
11.38


ATOM
2223
CD1
PHE
A
289
−7.976
25.114
−10.367
1.00
12.17


ATOM
2224
CE1
PHE
A
289
−7.184
24.095
−10.905
1.00
13.93


ATOM
2225
CZ
PHE
A
289
−7.572
22.783
−10.771
1.00
13.96


ATOM
2226
CE2
PHE
A
289
−8.756
22.452
−10.097
1.00
11.77


ATOM
2227
CD2
PHE
A
289
−9.555
23.472
−9.571
1.00
11.08


ATOM
2228
C
PHE
A
289
−10.219
27.698
−7.491
1.00
14.73


ATOM
2229
O
PHE
A
289
−10.092
28.713
−8.189
1.00
14.71


ATOM
2230
N
ARG
A
290
−11.054
27.621
−6.464
1.00
15.09


ATOM
2231
CA
ARG
A
290
−11.953
28.740
−6.140
1.00
16.64


ATOM
2232
CB
ARG
A
290
−12.842
28.401
−4.936
1.00
15.99


ATOM
2233
CG
ARG
A
290
−13.913
27.375
−5.230
1.00
15.65


ATOM
2234
CD
ARG
A
290
−14.821
27.163
−4.012
1.00
16.79


ATOM
2235
NE
ARG
A
290
−15.843
26.172
−4.330
1.00
15.04


ATOM
2236
CZ
ARG
A
290
−16.986
26.470
−4.933
1.00
17.22


ATOM
2237
NH1
ARG
A
290
−17.248
27.734
−5.243
1.00
15.41


ATOM
2238
NH2
ARG
A
290
−17.855
25.511
−5.239
1.00
15.94


ATOM
2239
C
ARG
A
290
−11.240
30.046
−5.864
1.00
18.04


ATOM
2240
O
ARG
A
290
−11.690
31.125
−6.279
1.00
19.61


ATOM
2241
N
SER
A
291
−10.150
29.984
−5.128
1.00
19.44


ATOM
2242
CA
SER
A
291
−9.571
31.246
−4.667
1.00
21.57


ATOM
2243
CB
SER
A
291
−9.146
31.101
−3.212
1.00
22.18


ATOM
2244
OG
SER
A
291
−7.998
30.284
−3.144
1.00
28.35


ATOM
2245
C
SER
A
291
−8.423
31.762
−5.534
1.00
20.65


ATOM
2246
O
SER
A
291
−7.865
32.851
−5.272
1.00
22.43


ATOM
2247
N
ILE
A
292
−8.066
31.019
−6.576
1.00
19.16


ATOM
2248
CA
ILE
A
292
−6.855
31.367
−7.330
1.00
17.65


ATOM
2249
CB
ILE
A
292
−5.805
30.185
−7.408
1.00
18.07


ATOM
2250
CG1
ILE
A
292
−6.379
28.972
−8.194
1.00
17.67


ATOM
2251
CD1
ILE
A
292
−5.315
27.924
−8.649
1.00
17.27


ATOM
2252
CG2
ILE
A
292
−5.341
29.795
−5.994
1.00
18.29


ATOM
2253
C
ILE
A
292
−7.065
31.973
−8.708
1.00
17.20


ATOM
2254
O
ILE
A
292
−6.136
32.563
−9.251
1.00
16.35


ATOM
2255
N
TYR
A
293
−8.252
31.797
−9.290
1.00
15.85


ATOM
2256
CA
TYR
A
293
−8.509
32.304
−10.648
1.00
15.83


ATOM
2257
CB
TYR
A
293
−9.301
31.270
−11.474
1.00
15.43


ATOM
2258
CG
TYR
A
293
−8.571
30.014
−11.886
1.00
15.10


ATOM
2259
CD1
TYR
A
293
−7.183
29.960
−11.965
1.00
14.38


ATOM
2260
CE1
TYR
A
293
−6.540
28.795
−12.395
1.00
14.38


ATOM
2261
CZ
TYR
A
293
−7.306
27.685
−12.743
1.00
14.90


ATOM
2262
OH
TYR
A
293
−6.700
26.522
−13.158
1.00
15.55


ATOM
2263
CE2
TYR
A
293
−8.670
27.722
−12.671
1.00
15.47


ATOM
2264
CD2
TYR
A
293
−9.298
28.875
−12.255
1.00
13.91


ATOM
2265
C
TYR
A
293
−9.351
33.581
−10.591
1.00
15.69


ATOM
2266
O
TYR
A
293
−10.404
33.594
−9.942
1.00
15.47


ATOM
2267
N
GLY
A
294
−8.892
34.629
−11.276
1.00
14.83


ATOM
2268
CA
GLY
A
294
−9.641
35.899
−11.353
1.00
15.57


ATOM
2269
C
GLY
A
294
−11.078
35.702
−11.858
1.00
15.93


ATOM
2270
O
GLY
A
294
−12.010
36.359
−11.376
1.00
15.66


ATOM
2271
N
VAL
A
295
−11.288
34.773
−12.799
1.00
15.60


ATOM
2272
CA
VAL
A
295
−12.651
34.520
−13.270
1.00
16.24


ATOM
2273
CB
VAL
A
295
−12.753
33.561
−14.501
1.00
16.31


ATOM
2274
CG1
VAL
A
295
−12.170
34.195
−15.740
1.00
16.26


ATOM
2275
CG2
VAL
A
295
−12.128
32.184
−14.199
1.00
16.19


ATOM
2276
C
VAL
A
295
−13.596
34.013
−12.172
1.00
16.97


ATOM
2277
O
VAL
A
295
−14.813
34.108
−12.320
1.00
18.03


ATOM
2278
N
ASN
A
296
−13.047
33.463
−11.092
1.00
16.93


ATOM
2279
CA
ASN
A
296
−13.878
32.920
−10.020
1.00
17.69


ATOM
2280
CB
ASN
A
296
−13.250
31.633
−9.472
1.00
17.57


ATOM
2281
CG
ASN
A
296
−13.296
30.493
−10.482
1.00
16.44


ATOM
2282
OD1
ASN
A
296
−14.158
30.481
−11.356
1.00
17.29


ATOM
2283
ND2
ASN
A
296
−12.401
29.513
−10.336
1.00
15.99


ATOM
2284
C
ASN
A
296
−14.187
33.915
−8.896
1.00
19.30


ATOM
2285
O
ASN
A
296
−14.945
33.601
−7.979
1.00
19.07


ATOM
2286
N
LYS
A
297
−13.617
35.116
−9.007
1.00
20.37


ATOM
2287
CA
LYS
A
297
−13.811
36.203
−8.038
1.00
22.43


ATOM
2288
CB
LYS
A
297
−13.209
37.502
−8.584
1.00
22.90


ATOM
2289
CG
LYS
A
297
−11.741
37.680
−8.316
1.00
30.03


ATOM
2290
CD
LYS
A
297
−11.401
39.189
−8.309
1.00
35.34


ATOM
2291
CE
LYS
A
297
−12.247
39.913
−7.255
1.00
39.86


ATOM
2292
NZ
LYS
A
297
−11.995
41.386
−7.178
1.00
42.72


ATOM
2293
C
LYS
A
297
−15.275
36.453
−7.782
1.00
21.89


ATOM
2294
O
LYS
A
297
−16.061
36.585
−8.712
1.00
21.96


ATOM
2295
N
GLY
A
298
−15.659
36.537
−6.517
1.00
22.72


ATOM
2296
CA
GLY
A
298
−17.050
36.869
−6.219
1.00
22.99


ATOM
2297
C
GLY
A
298
−18.043
35.720
−6.278
1.00
23.54


ATOM
2298
O
GLY
A
298
−19.180
35.885
−5.855
1.00
25.04


ATOM
2299
N
ILE
A
299
−17.647
34.546
−6.784
1.00
22.16


ATOM
2300
CA
ILE
A
299
−18.574
33.393
−6.763
1.00
21.47


ATOM
2301
CB
ILE
A
299
−18.251
32.350
−7.884
1.00
21.22


ATOM
2302
CG1
ILE
A
299
−18.356
32.985
−9.274
1.00
19.64


ATOM
2303
CD1
ILE
A
299
−17.740
32.095
−10.415
1.00
19.76


ATOM
2304
CG2
ILE
A
299
−19.163
31.091
−7.762
1.00
20.54


ATOM
2305
C
ILE
A
299
−18.562
32.740
−5.375
1.00
22.34


ATOM
2306
O
ILE
A
299
−17.486
32.395
−4.861
1.00
22.29


ATOM
2307
N
PRO
A
300
−19.743
32.580
−4.751
1.00
23.04


ATOM
2308
CA
PRO
A
300
−19.791
32.018
−3.392
1.00
23.60


ATOM
2309
CB
PRO
A
300
−21.217
32.364
−2.922
1.00
24.19


ATOM
2310
CG
PRO
A
300
−22.015
32.437
−4.178
1.00
23.80


ATOM
2311
CD
PRO
A
300
−21.085
32.934
−5.253
1.00
23.06


ATOM
2312
C
PRO
A
300
−19.584
30.500
−3.322
1.00
23.50


ATOM
2313
O
PRO
A
300
−19.664
29.810
−4.347
1.00
22.46


ATOM
2314
N
ALA
A
301
−19.325
29.985
−2.116
1.00
22.68


ATOM
2315
CA
ALA
A
301
−19.380
28.549
−1.905
1.00
22.89


ATOM
2316
CB
ALA
A
301
−18.988
28.185
−0.465
1.00
23.54


ATOM
2317
C
ALA
A
301
−20.788
28.074
−2.236
1.00
21.91


ATOM
2318
O
ALA
A
301
−21.759
28.834
−2.108
1.00
23.09


ATOM
2319
N
GLY
A
302
−20.898
26.838
−2.698
1.00
20.96


ATOM
2320
CA
GLY
A
302
−22.173
26.272
−3.115
1.00
19.69


ATOM
2321
C
GLY
A
302
−22.565
26.637
−4.537
1.00
19.78


ATOM
2322
O
GLY
A
302
−23.661
26.283
−4.991
1.00
19.32


ATOM
2323
N
ALA
A
303
−21.686
27.355
−5.235
1.00
17.97


ATOM
2324
CA
ALA
A
303
−21.948
27.708
−6.635
1.00
17.13


ATOM
2325
CB
ALA
A
303
−22.168
29.212
−6.812
1.00
16.73


ATOM
2326
C
ALA
A
303
−20.784
27.245
−7.481
1.00
16.19


ATOM
2327
O
ALA
A
303
−19.647
27.171
−7.004
1.00
16.23


ATOM
2328
N
ALA
A
304
−21.067
26.956
−8.746
1.00
15.66


ATOM
2329
CA
ALA
A
304
−20.069
26.378
−9.640
1.00
15.10


ATOM
2330
CB
ALA
A
304
−20.750
25.795
−10.860
1.00
15.80


ATOM
2331
C
ALA
A
304
−19.002
27.394
−10.044
1.00
14.74


ATOM
2332
O
ALA
A
304
−19.270
28.587
−10.121
1.00
14.27


ATOM
2333
N
VAL
A
305
−17.783
26.914
−10.300
1.00
14.18


ATOM
2334
CA
VAL
A
305
−16.680
27.783
−10.698
1.00
13.54


ATOM
2335
CB
VAL
A
305
−15.656
27.971
−9.543
1.00
13.42


ATOM
2336
CG1
VAL
A
305
−16.224
28.881
−8.418
1.00
14.25


ATOM
2337
CG2
VAL
A
305
−15.218
26.597
−8.966
1.00
14.99


ATOM
2338
C
VAL
A
305
−15.952
27.141
−11.873
1.00
13.34


ATOM
2339
O
VAL
A
305
−16.121
25.944
−12.126
1.00
12.46


ATOM
2340
N
ALA
A
306
−15.130
27.921
−12.562
1.00
13.38


ATOM
2341
CA
ALA
A
306
−14.233
27.376
−13.573
1.00
14.37


ATOM
2342
CB
ALA
A
306
−13.709
28.504
−14.470
1.00
15.32


ATOM
2343
C
ALA
A
306
−13.082
26.626
−12.938
1.00
14.68


ATOM
2344
O
ALA
A
306
−12.457
27.116
−11.974
1.00
15.21


ATOM
2345
N
ILE
A
307
−12.781
25.452
−13.484
1.00
13.50


ATOM
2346
CA
ILE
A
307
−11.667
24.668
−12.975
1.00
13.67


ATOM
2347
CB
ILE
A
307
−12.134
23.438
−12.163
1.00
14.45


ATOM
2348
CG1
ILE
A
307
−12.756
22.386
−13.072
1.00
15.37


ATOM
2349
CD1
ILE
A
307
−12.921
21.033
−12.368
1.00
19.15


ATOM
2350
CG2
ILE
A
307
−13.119
23.848
−11.005
1.00
15.52


ATOM
2351
C
ILE
A
307
−10.646
24.290
−14.059
1.00
12.52


ATOM
2352
O
ILE
A
307
−10.974
24.232
−15.264
1.00
12.22


ATOM
2353
N
GLY
A
308
−9.405
24.095
−13.604
1.00
11.71


ATOM
2354
CA
GLY
A
308
−8.276
23.737
−14.452
1.00
11.64


ATOM
2355
C
GLY
A
308
−7.853
22.306
−14.199
1.00
11.62


ATOM
2356
O
GLY
A
308
−8.667
21.444
−13.806
1.00
12.05


ATOM
2357
N
ARG
A
309
−6.583
22.026
−14.454
1.00
11.39


ATOM
2358
CA
ARG
A
309
−6.091
20.661
−14.337
1.00
11.23


ATOM
2359
CB
ARG
A
309
−4.896
20.467
−15.275
1.00
11.37


ATOM
2360
CG
ARG
A
309
−5.220
20.697
−16.791
1.00
11.29


ATOM
2361
CD
ARG
A
309
−4.066
20.130
−17.625
1.00
12.62


ATOM
2362
NE
ARG
A
309
−2.845
20.919
−17.425
1.00
12.15


ATOM
2363
CZ
ARG
A
309
−1.701
20.665
−18.047
1.00
15.00


ATOM
2364
NH1
ARG
A
309
−1.630
19.633
−18.910
1.00
12.05


ATOM
2365
NH2
ARG
A
309
−0.624
21.395
−17.778
1.00
13.85


ATOM
2366
C
ARG
A
309
−5.654
20.425
−12.888
1.00
11.83


ATOM
2367
O
ARG
A
309
−6.093
19.481
−12.221
1.00
11.38


ATOM
2368
N
TYR
A
310
−4.806
21.322
−12.399
1.00
11.88


ATOM
2369
CA
TYR
A
310
−4.293
21.215
−11.022
1.00
11.17


ATOM
2370
CB
TYR
A
310
−3.225
20.082
−10.878
1.00
12.49


ATOM
2371
CG
TYR
A
310
−2.065
20.201
−11.844
1.00
13.10


ATOM
2372
CD1
TYR
A
310
−2.128
19.622
−13.138
1.00
12.57


ATOM
2373
CE1
TYR
A
310
−1.069
19.772
−14.039
1.00
15.63


ATOM
2374
CZ
TYR
A
310
0.065
20.475
−13.649
1.00
14.31


ATOM
2375
OH
TYR
A
310
1.119
20.611
−14.529
1.00
14.42


ATOM
2376
CE2
TYR
A
310
0.159
21.030
−12.379
1.00
12.68


ATOM
2377
CD2
TYR
A
310
−0.909
20.906
−11.485
1.00
14.04


ATOM
2378
C
TYR
A
310
−3.779
22.596
−10.644
1.00
12.74


ATOM
2379
O
TYR
A
310
−3.333
23.356
−11.505
1.00
12.44


ATOM
2380
N
ALA
A
311
−3.872
22.945
−9.362
1.00
11.99


ATOM
2381
CA
ALA
A
311
−3.618
24.337
−8.975
1.00
13.33


ATOM
2382
CB
ALA
A
311
−4.084
24.589
−7.508
1.00
12.88


ATOM
2383
C
ALA
A
311
−2.157
24.768
−9.197
1.00
13.50


ATOM
2384
O
ALA
A
311
−1.906
25.951
−9.468
1.00
14.52


ATOM
2385
N
GLU
A
312
−1.216
23.823
−9.140
1.00
13.52


ATOM
2386
CA
GLU
A
312
0.219
24.134
−9.332
1.00
14.23


ATOM
2387
CB
GLU
A
312
1.111
23.020
−8.790
1.00
15.44


ATOM
2388
CG
GLU
A
312
0.933
22.802
−7.303
1.00
16.54


ATOM
2389
CD
GLU
A
312
−0.130
21.762
−6.950
1.00
18.91


ATOM
2390
OE1
GLU
A
312
−0.941
21.338
−7.808
1.00
16.89


ATOM
2391
OE2
GLU
A
312
−0.150
21.345
−5.778
1.00
18.72


ATOM
2392
C
GLU
A
312
0.591
24.380
−10.796
1.00
14.80


ATOM
2393
O
GLU
A
312
1.741
24.736
−11.100
1.00
14.98


ATOM
2394
N
ASP
A
313
−0.374
24.197
−11.697
1.00
13.37


ATOM
2395
CA
ASP
A
313
−0.112
24.258
−13.155
1.00
13.71


ATOM
2396
CB
ASP
A
313
−1.457
24.079
−13.888
1.00
12.88


ATOM
2397
CG
ASP
A
313
−1.320
23.671
−15.343
1.00
14.44


ATOM
2398
OD1
ASP
A
313
−0.197
23.597
−15.900
1.00
13.19


ATOM
2399
OD2
ASP
A
313
−2.400
23.406
−15.923
1.00
13.61


ATOM
2400
C
ASP
A
313
0.512
25.589
−13.587
1.00
14.00


ATOM
2401
O
ASP
A
313
0.007
26.662
−13.219
1.00
14.35


ATOM
2402
N
VAL
A
314
1.577
25.530
−14.399
1.00
13.84


ATOM
2403
CA
VAL
A
314
2.145
26.747
−14.988
1.00
15.19


ATOM
2404
CB
VAL
A
314
3.602
27.016
−14.520
1.00
16.70


ATOM
2405
CG1
VAL
A
314
3.638
27.295
−13.009
1.00
17.94


ATOM
2406
CG2
VAL
A
314
4.551
25.857
−14.915
1.00
16.69


ATOM
2407
C
VAL
A
314
2.123
26.729
−16.528
1.00
15.30


ATOM
2408
O
VAL
A
314
2.712
27.598
−17.165
1.00
15.13


ATOM
2409
N
TYR
A
315
1.441
25.743
−17.111
1.00
14.51


ATOM
2410
CA
TYR
A
315
1.351
25.634
−18.580
1.00
15.65


ATOM
2411
CB
TYR
A
315
0.768
24.264
−18.957
1.00
15.82


ATOM
2412
CG
TYR
A
315
0.694
23.988
−20.457
1.00
16.29


ATOM
2413
CD1
TYR
A
315
1.824
24.124
−21.265
1.00
17.01


ATOM
2414
CE1
TYR
A
315
1.778
23.859
−22.634
1.00
18.92


ATOM
2415
CZ
TYR
A
315
0.588
23.421
−23.208
1.00
16.14


ATOM
2416
OH
TYR
A
315
0.557
23.164
−24.577
1.00
16.95


ATOM
2417
CE2
TYR
A
315
−0.552
23.261
−22.423
1.00
15.41


ATOM
2418
CD2
TYR
A
315
−0.492
23.539
−21.044
1.00
14.48


ATOM
2419
C
TYR
A
315
0.489
26.777
−19.107
1.00
15.56


ATOM
2420
O
TYR
A
315
−0.688
26.888
−18.748
1.00
16.36


ATOM
2421
N
TYR
A
316
1.072
27.645
−19.944
1.00
16.61


ATOM
2422
CA
TYR
A
316
0.404
28.890
−20.380
1.00
17.53


ATOM
2423
CB
TYR
A
316
−0.778
28.603
−21.337
1.00
18.31


ATOM
2424
CG
TYR
A
316
−0.329
28.321
−22.742
1.00
19.74


ATOM
2425
CD1
TYR
A
316
−0.071
27.026
−23.169
1.00
18.95


ATOM
2426
CE1
TYR
A
316
0.353
26.757
−24.466
1.00
18.69


ATOM
2427
CZ
TYR
A
316
0.551
27.812
−25.342
1.00
21.77


ATOM
2428
OH
TYR
A
316
1.002
27.557
−26.617
1.00
23.75


ATOM
2429
CE2
TYR
A
316
0.329
29.125
−24.932
1.00
22.39


ATOM
2430
CD2
TYR
A
316
−0.111
29.369
−23.639
1.00
21.90


ATOM
2431
C
TYR
A
316
−0.037
29.730
−19.173
1.00
17.87


ATOM
2432
O
TYR
A
316
−0.968
30.517
−19.266
1.00
17.06


ATOM
2433
N
ASN
A
317
0.689
29.555
−18.066
1.00
18.34


ATOM
2434
CA
ASN
A
317
0.483
30.231
−16.766
1.00
19.27


ATOM
2435
CB
ASN
A
317
0.106
31.699
−16.921
1.00
20.01


ATOM
2436
CG
ASN
A
317
1.171
32.489
−17.624
1.00
24.51


ATOM
2437
OD1
ASN
A
317
2.363
32.384
−17.305
1.00
29.46


ATOM
2438
ND2
ASN
A
317
0.756
33.269
−18.603
1.00
29.08


ATOM
2439
C
ASN
A
317
−0.506
29.551
−15.842
1.00
18.28


ATOM
2440
O
ASN
A
317
−0.706
30.001
−14.719
1.00
19.05


ATOM
2441
N
GLY
A
318
−1.114
28.459
−16.300
1.00
17.74


ATOM
2442
CA
GLY
A
318
−2.086
27.721
−15.475
1.00
15.34


ATOM
2443
C
GLY
A
318
−3.458
28.356
−15.550
1.00
15.47


ATOM
2444
O
GLY
A
318
−3.700
29.390
−14.932
1.00
15.75


ATOM
2445
N
ASN
A
319
−4.369
27.733
−16.306
1.00
13.16


ATOM
2446
CA
ASN
A
319
−5.672
28.305
−16.557
1.00
12.74


ATOM
2447
CB
ASN
A
319
−5.693
28.883
−17.980
1.00
12.31


ATOM
2448
CG
ASN
A
319
−4.676
29.979
−18.187
1.00
13.01


ATOM
2449
OD1
ASN
A
319
−4.832
31.117
−17.699
1.00
14.18


ATOM
2450
ND2
ASN
A
319
−3.640
29.665
−18.942
1.00
11.49


ATOM
2451
C
ASN
A
319
−6.799
27.271
−16.442
1.00
12.27


ATOM
2452
O
ASN
A
319
−6.545
26.071
−16.456
1.00
12.28


ATOM
2453
N
PRO
A
320
−8.054
27.732
−16.334
1.00
12.96


ATOM
2454
CA
PRO
A
320
−9.113
26.759
−16.472
1.00
12.58


ATOM
2455
CB
PRO
A
320
−10.395
27.579
−16.324
1.00
13.28


ATOM
2456
CG
PRO
A
320
−10.007
29.011
−16.183
1.00
14.34


ATOM
2457
CD
PRO
A
320
−8.537
29.090
−15.991
1.00
12.66


ATOM
2458
C
PRO
A
320
−9.101
26.090
−17.851
1.00
12.18


ATOM
2459
O
PRO
A
320
−8.643
26.698
−18.820
1.00
11.99


ATOM
2460
N
TRP
A
321
−9.589
24.852
−17.912
1.00
11.79


ATOM
2461
CA
TRP
A
321
−9.739
24.116
−19.154
1.00
11.97


ATOM
2462
CB
TRP
A
321
−8.988
22.775
−19.063
1.00
11.15


ATOM
2463
CG
TRP
A
321
−7.469
22.900
−19.200
1.00
12.16


ATOM
2464
CD1
TRP
A
321
−6.658
23.837
−18.627
1.00
13.28


ATOM
2465
NE1
TRP
A
321
−5.347
23.636
−19.016
1.00
13.24


ATOM
2466
CE2
TRP
A
321
−5.290
22.538
−19.831
1.00
13.51


ATOM
2467
CD2
TRP
A
321
−6.617
22.054
−19.978
1.00
12.68


ATOM
2468
CE3
TRP
A
321
−6.846
20.938
−20.787
1.00
14.41


ATOM
2469
CZ3
TRP
A
321
−5.741
20.323
−21.428
1.00
13.49


ATOM
2470
CH2
TRP
A
321
−4.436
20.819
−21.250
1.00
13.43


ATOM
2471
CZ2
TRP
A
321
−4.193
21.948
−20.479
1.00
14.69


ATOM
2472
C
TRP
A
321
−11.202
23.797
−19.342
1.00
11.99


ATOM
2473
O
TRP
A
321
−11.875
23.448
−18.388
1.00
11.51


ATOM
2474
N
TYR
A
322
−11.696
23.896
−20.579
1.00
11.47


ATOM
2475
CA
TYR
A
322
−13.088
23.511
−20.841
1.00
11.31


ATOM
2476
CB
TYR
A
322
−13.433
23.691
−22.322
1.00
12.14


ATOM
2477
CG
TYR
A
322
−13.352
25.130
−22.793
1.00
12.81


ATOM
2478
CD1
TYR
A
322
−12.260
25.574
−23.509
1.00
11.43


ATOM
2479
CE1
TYR
A
322
−12.173
26.914
−23.965
1.00
12.91


ATOM
2480
CZ
TYR
A
322
−13.216
27.802
−23.697
1.00
14.27


ATOM
2481
OH
TYR
A
322
−13.127
29.104
−24.146
1.00
15.10


ATOM
2482
CE2
TYR
A
322
−14.324
27.373
−22.982
1.00
13.40


ATOM
2483
CD2
TYR
A
322
−14.378
26.031
−22.522
1.00
11.58


ATOM
2484
C
TYR
A
322
−13.367
22.082
−20.433
1.00
11.31


ATOM
2485
O
TYR
A
322
−14.380
21.795
−19.771
1.00
11.08


ATOM
2486
N
LEU
A
323
−12.480
21.169
−20.814
1.00
10.96


ATOM
2487
CA
LEU
A
323
−12.770
19.750
−20.561
1.00
11.04


ATOM
2488
CB
LEU
A
323
−11.787
18.844
−21.315
1.00
11.26


ATOM
2489
CG
LEU
A
323
−10.314
18.876
−20.903
1.00
10.53


ATOM
2490
CD1
LEU
A
323
−10.074
17.902
−19.745
1.00
14.57


ATOM
2491
CD2
LEU
A
323
−9.474
18.437
−22.112
1.00
13.19


ATOM
2492
C
LEU
A
323
−12.778
19.449
−19.048
1.00
11.68


ATOM
2493
O
LEU
A
323
−13.444
18.510
−18.602
1.00
12.06


ATOM
2494
N
ALA
A
324
−12.036
20.239
−18.268
1.00
9.86


ATOM
2495
CA
ALA
A
324
−11.969
20.017
−16.812
1.00
10.09


ATOM
2496
CB
ALA
A
324
−10.746
20.767
−16.234
1.00
9.76


ATOM
2497
C
ALA
A
324
−13.272
20.518
−16.178
1.00
10.17


ATOM
2498
O
ALA
A
324
−13.866
19.840
−15.325
1.00
10.29


ATOM
2499
N
THR
A
325
−13.758
21.662
−16.665
1.00
9.84


ATOM
2500
CA
THR
A
325
−15.000
22.267
−16.172
1.00
11.14


ATOM
2501
CB
THR
A
325
−15.102
23.765
−16.623
1.00
12.15


ATOM
2502
OG1
THR
A
325
−14.002
24.498
−16.063
1.00
13.16


ATOM
2503
CG2
THR
A
325
−16.402
24.411
−16.152
1.00
11.83


ATOM
2504
C
THR
A
325
−16.218
21.413
−16.570
1.00
11.50


ATOM
2505
O
THR
A
325
−17.086
21.126
−15.727
1.00
10.79


ATOM
2506
N
PHE
A
326
−16.234
20.925
−17.816
1.00
10.79


ATOM
2507
CA
PHE
A
326
−17.272
19.959
−18.240
1.00
12.12


ATOM
2508
CB
PHE
A
326
−17.194
19.652
−19.746
1.00
12.14


ATOM
2509
CG
PHE
A
326
−17.518
20.851
−20.640
1.00
13.71


ATOM
2510
CD1
PHE
A
326
−16.777
21.077
−21.804
1.00
15.21


ATOM
2511
CE1
PHE
A
326
−17.043
22.188
−22.635
1.00
14.99


ATOM
2512
CZ
PHE
A
326
−18.072
23.066
−22.311
1.00
16.33


ATOM
2513
CE2
PHE
A
326
−18.851
22.832
−21.160
1.00
20.12


ATOM
2514
CD2
PHE
A
326
−18.561
21.717
−20.331
1.00
16.63


ATOM
2515
C
PHE
A
326
−17.216
18.643
−17.464
1.00
11.56


ATOM
2516
O
PHE
A
326
−18.263
18.069
−17.180
1.00
11.74


ATOM
2517
N
ALA
A
327
−16.014
18.174
−17.103
1.00
11.35


ATOM
2518
CA
ALA
A
327
−15.889
16.909
−16.346
1.00
11.41


ATOM
2519
CB
ALA
A
327
−14.397
16.538
−16.158
1.00
11.95


ATOM
2520
C
ALA
A
327
−16.612
16.964
−14.965
1.00
12.04


ATOM
2521
O
ALA
A
327
−17.260
15.985
−14.561
1.00
12.69


ATOM
2522
N
ALA
A
328
−16.505
18.097
−14.266
1.00
12.13


ATOM
2523
CA
ALA
A
328
−17.207
18.293
−12.985
1.00
12.24


ATOM
2524
CB
ALA
A
328
−16.871
19.662
−12.369
1.00
12.48


ATOM
2525
C
ALA
A
328
−18.707
18.157
−13.177
1.00
12.90


ATOM
2526
O
ALA
A
328
−19.378
17.454
−12.411
1.00
13.60


ATOM
2527
N
ALA
A
329
−19.239
18.814
−14.202
1.00
12.55


ATOM
2528
CA
ALA
A
329
−20.669
18.682
−14.504
1.00
12.31


ATOM
2529
CB
ALA
A
329
−21.027
19.551
−15.692
1.00
12.87


ATOM
2530
C
ALA
A
329
−21.035
17.226
−14.788
1.00
12.71


ATOM
2531
O
ALA
A
329
−22.016
16.700
−14.266
1.00
12.32


ATOM
2532
N
GLU
A
330
−20.231
16.572
−15.629
1.00
12.54


ATOM
2533
CA
GLU
A
330
−20.500
15.187
−16.003
1.00
12.84


ATOM
2534
CB
GLU
A
330
−19.519
14.718
−17.100
1.00
12.55


ATOM
2535
CG
GLU
A
330
−19.850
13.303
−17.626
1.00
13.80


ATOM
2536
CD
GLU
A
330
−19.108
12.953
−18.917
1.00
13.54


ATOM
2537
OE1
GLU
A
330
−18.650
13.889
−19.604
1.00
12.29


ATOM
2538
OE2
GLU
A
330
−18.998
11.739
−19.209
1.00
14.52


ATOM
2539
C
GLU
A
330
−20.523
14.231
−14.809
1.00
12.94


ATOM
2540
O
GLU
A
330
−21.400
13.346
−14.726
1.00
12.90


ATOM
2541
N
GLN
A
331
−19.598
14.402
−13.866
1.00
12.03


ATOM
2542
CA
GLN
A
331
−19.589
13.502
−12.726
1.00
12.38


ATOM
2543
CB
GLN
A
331
−18.415
13.795
−11.797
1.00
12.24


ATOM
2544
CG
GLN
A
331
−18.357
12.759
−10.670
1.00
13.61


ATOM
2545
CD
GLN
A
331
−17.327
13.072
−9.608
1.00
15.82


ATOM
2546
OE1
GLN
A
331
−16.263
13.617
−9.895
1.00
15.39


ATOM
2547
NE2
GLN
A
331
−17.628
12.702
−8.372
1.00
13.76


ATOM
2548
C
GLN
A
331
−20.912
13.643
−11.969
1.00
12.33


ATOM
2549
O
GLN
A
331
−21.512
12.659
−11.556
1.00
12.45


ATOM
2550
N
LEU
A
332
−21.377
14.873
−11.844
1.00
12.57


ATOM
2551
CA
LEU
A
332
−22.628
15.138
−11.134
1.00
13.59


ATOM
2552
CB
LEU
A
332
−22.747
16.631
−10.868
1.00
13.17


ATOM
2553
CG
LEU
A
332
−21.681
17.142
−9.867
1.00
16.56


ATOM
2554
CD1
LEU
A
332
−21.718
18.678
−9.801
1.00
18.10


ATOM
2555
CD2
LEU
A
332
−21.851
16.476
−8.492
1.00
19.47


ATOM
2556
C
LEU
A
332
−23.861
14.600
−11.864
1.00
13.57


ATOM
2557
O
LEU
A
332
−24.770
14.053
−11.239
1.00
13.24


ATOM
2558
N
TYR
A
333
−23.909
14.766
−13.179
1.00
13.90


ATOM
2559
CA
TYR
A
333
−24.988
14.131
−13.972
1.00
14.37


ATOM
2560
CB
TYR
A
333
−24.901
14.523
−15.468
1.00
14.48


ATOM
2561
CG
TYR
A
333
−25.056
16.001
−15.721
1.00
13.91


ATOM
2562
CD1
TYR
A
333
−26.086
16.738
−15.118
1.00
14.64


ATOM
2563
CE1
TYR
A
333
−26.208
18.117
−15.350
1.00
15.65


ATOM
2564
CZ
TYR
A
333
−25.315
18.758
−16.196
1.00
16.47


ATOM
2565
OH
TYR
A
333
−25.431
20.101
−16.442
1.00
17.22


ATOM
2566
CE2
TYR
A
333
−24.310
18.050
−16.836
1.00
16.59


ATOM
2567
CD2
TYR
A
333
−24.192
16.669
−16.601
1.00
11.27


ATOM
2568
C
TYR
A
333
−25.022
12.613
−13.843
1.00
15.03


ATOM
2569
O
TYR
A
333
−26.108
12.012
−13.824
1.00
14.78


ATOM
2570
N
ASP
A
334
−23.836
11.998
−13.807
1.00
14.06


ATOM
2571
CA
ASP
A
334
−23.714
10.555
−13.602
1.00
14.83


ATOM
2572
CB
ASP
A
334
−22.239
10.114
−13.714
1.00
13.83


ATOM
2573
CG
ASP
A
334
−21.708
10.149
−15.136
1.00
15.84


ATOM
2574
OD1
ASP
A
334
−22.495
10.373
−16.081
1.00
13.95


ATOM
2575
OD2
ASP
A
334
−20.470
9.943
−15.313
1.00
15.50


ATOM
2576
C
ASP
A
334
−24.254
10.163
−12.224
1.00
15.21


ATOM
2577
O
ASP
A
334
−24.941
9.132
−12.080
1.00
15.93


ATOM
2578
N
ALA
A
335
−23.933
10.969
−11.213
1.00
15.09


ATOM
2579
CA
ALA
A
335
−24.454
10.735
−9.855
1.00
16.00


ATOM
2580
CB
ALA
A
335
−23.809
11.719
−8.864
1.00
15.13


ATOM
2581
C
ALA
A
335
−25.980
10.823
−9.803
1.00
16.10


ATOM
2582
O
ALA
A
335
−26.643
9.916
−9.245
1.00
16.77


ATOM
2583
N
ILE
A
336
−26.530
11.879
−10.398
1.00
16.35


ATOM
2584
CA
ILE
A
336
−27.987
12.087
−10.470
1.00
18.39


ATOM
2585
CB
ILE
A
336
−28.332
13.422
−11.162
1.00
18.85


ATOM
2586
CG1
ILE
A
336
−27.891
14.596
−10.279
1.00
19.14


ATOM
2587
CD1
ILE
A
336
−27.879
15.904
−10.986
1.00
22.50


ATOM
2588
CG2
ILE
A
336
−29.839
13.539
−11.506
1.00
20.14


ATOM
2589
C
ILE
A
336
−28.681
10.902
−11.156
1.00
18.83


ATOM
2590
O
ILE
A
336
−29.707
10.404
−10.675
1.00
18.06


ATOM
2591
N
TYR
A
337
−28.102
10.443
−12.267
1.00
18.50


ATOM
2592
CA
TYR
A
337
−28.642
9.287
−12.970
1.00
18.99


ATOM
2593
CB
TYR
A
337
−27.753
8.908
−14.169
1.00
19.80


ATOM
2594
CG
TYR
A
337
−28.328
7.737
−14.954
1.00
20.76


ATOM
2595
CD1
TYR
A
337
−27.988
6.429
−14.620
1.00
20.95


ATOM
2596
CE1
TYR
A
337
−28.511
5.345
−15.322
1.00
22.94


ATOM
2597
CZ
TYR
A
337
−29.382
5.559
−16.356
1.00
22.19


ATOM
2598
OH
TYR
A
337
−29.877
4.447
−17.018
1.00
24.87


ATOM
2599
CE2
TYR
A
337
−29.752
6.845
−16.721
1.00
22.31


ATOM
2600
CD2
TYR
A
337
−29.220
7.942
−16.009
1.00
21.58


ATOM
2601
C
TYR
A
337
−28.839
8.083
−12.057
1.00
18.60


ATOM
2602
O
TYR
A
337
−29.918
7.476
−12.041
1.00
18.61


ATOM
2603
N
VAL
A
338
−27.802
7.737
−11.297
1.00
18.67


ATOM
2604
CA
VAL
A
338
−27.837
6.573
−10.406
1.00
18.90


ATOM
2605
CB
VAL
A
338
−26.424
6.195
−9.919
1.00
18.99


ATOM
2606
CG1
VAL
A
338
−26.462
5.121
−8.820
1.00
19.71


ATOM
2607
CG2
VAL
A
338
−25.600
5.698
−11.111
1.00
18.75


ATOM
2608
C
VAL
A
338
−28.810
6.788
−9.234
1.00
19.41


ATOM
2609
O
VAL
A
338
−29.565
5.871
−8.869
1.00
19.45


ATOM
2610
N
TRP
A
339
−28.797
7.987
−8.654
1.00
19.81


ATOM
2611
CA
TRP
A
339
−29.743
8.290
−7.559
1.00
20.46


ATOM
2612
CB
TRP
A
339
−29.514
9.705
−7.029
1.00
20.35


ATOM
2613
CG
TRP
A
339
−28.222
9.830
−6.329
1.00
18.64


ATOM
2614
CD1
TRP
A
339
−27.540
8.846
−5.676
1.00
16.51


ATOM
2615
NE1
TRP
A
339
−26.391
9.359
−5.126
1.00
17.81


ATOM
2616
CE2
TRP
A
339
−26.312
10.693
−5.423
1.00
17.12


ATOM
2617
CD2
TRP
A
339
−27.452
11.025
−6.183
1.00
17.64


ATOM
2618
CE3
TRP
A
339
−27.624
12.343
−6.614
1.00
17.59


ATOM
2619
CZ3
TRP
A
339
−26.637
13.283
−6.284
1.00
19.24


ATOM
2620
CH2
TRP
A
339
−25.510
12.912
−5.520
1.00
18.24


ATOM
2621
CZ2
TRP
A
339
−25.320
11.626
−5.103
1.00
18.45


ATOM
2622
C
TRP
A
339
−31.201
8.108
−7.997
1.00
21.83


ATOM
2623
O
TRP
A
339
−31.981
7.478
−7.274
1.00
22.01


ATOM
2624
N
LYS
A
340
−31.549
8.646
−9.168
1.00
22.85


ATOM
2625
CA
LYS
A
340
−32.904
8.541
−9.721
1.00
25.61


ATOM
2626
CB
LYS
A
340
−33.066
9.411
−10.967
1.00
25.52


ATOM
2627
CG
LYS
A
340
−33.174
10.905
−10.689
1.00
28.19


ATOM
2628
CD
LYS
A
340
−33.227
11.692
−11.991
1.00
34.04


ATOM
2629
CE
LYS
A
340
−33.966
13.011
−11.805
1.00
38.04


ATOM
2630
NZ
LYS
A
340
−33.868
13.876
−13.017
1.00
41.83


ATOM
2631
C
LYS
A
340
−33.276
7.108
−10.062
1.00
27.14


ATOM
2632
O
LYS
A
340
−34.413
6.686
−9.830
1.00
27.56


ATOM
2633
N
LYS
A
341
−32.317
6.358
−10.604
1.00
28.13


ATOM
2634
CA
LYS
A
341
−32.552
4.975
−11.018
1.00
30.18


ATOM
2635
CB
LYS
A
341
−31.358
4.428
−11.800
1.00
29.83


ATOM
2636
CG
LYS
A
341
−31.688
3.173
−12.624
1.00
33.04


ATOM
2637
CD
LYS
A
341
−30.472
2.624
−13.395
1.00
33.62


ATOM
2638
CE
LYS
A
341
−29.652
1.592
−12.588
1.00
38.22


ATOM
2639
NZ
LYS
A
341
−28.691
2.188
−11.573
1.00
40.88


ATOM
2640
C
LYS
A
341
−32.816
4.081
−9.817
1.00
30.19


ATOM
2641
O
LYS
A
341
−33.744
3.260
−9.837
1.00
30.15


ATOM
2642
N
THR
A
342
−31.999
4.246
−8.777
1.00
29.52


ATOM
2643
CA
THR
A
342
−32.074
3.400
−7.595
1.00
29.75


ATOM
2644
CB
THR
A
342
−30.687
3.221
−6.916
1.00
29.68


ATOM
2645
OG1
THR
A
342
−30.254
4.458
−6.333
1.00
32.01


ATOM
2646
CG2
THR
A
342
−29.628
2.735
−7.929
1.00
31.40


ATOM
2647
C
THR
A
342
−33.129
3.901
−6.596
1.00
29.01


ATOM
2648
O
THR
A
342
−33.572
3.148
−5.734
1.00
29.92


ATOM
2649
N
GLY
A
343
−33.534
5.158
−6.732
1.00
28.20


ATOM
2650
CA
GLY
A
343
−34.537
5.782
−5.862
1.00
28.30


ATOM
2651
C
GLY
A
343
−34.068
6.045
−4.438
1.00
27.91


ATOM
2652
O
GLY
A
343
−34.887
6.133
−3.519
1.00
28.16


ATOM
2653
N
SER
A
344
−32.760
6.226
−4.260
1.00
27.22


ATOM
2654
CA
SER
A
344
−32.142
6.306
−2.939
1.00
26.60


ATOM
2655
CB
SER
A
344
−31.870
4.880
−2.462
1.00
27.28


ATOM
2656
OG
SER
A
344
−31.354
4.855
−1.161
1.00
29.50


ATOM
2657
C
SER
A
344
−30.823
7.107
−2.979
1.00
26.02


ATOM
2658
O
SER
A
344
−30.068
6.992
−3.944
1.00
25.80


ATOM
2659
N
ILE
A
345
−30.557
7.900
−1.936
1.00
24.20


ATOM
2660
CA
ILE
A
345
−29.295
8.641
−1.770
1.00
23.04


ATOM
2661
CB
ILE
A
345
−29.477
10.171
−1.954
1.00
23.03


ATOM
2662
CG1
ILE
A
345
−30.021
10.474
−3.340
1.00
22.44


ATOM
2663
CD1
ILE
A
345
−30.399
11.918
−3.599
1.00
23.24


ATOM
2664
CG2
ILE
A
345
−28.138
10.918
−1.670
1.00
22.00


ATOM
2665
C
ILE
A
345
−28.726
8.415
−0.378
1.00
23.13


ATOM
2666
O
ILE
A
345
−29.392
8.684
0.623
1.00
23.57


ATOM
2667
N
THR
A
346
−27.490
7.943
−0.307
1.00
22.23


ATOM
2668
CA
THR
A
346
−26.820
7.765
0.963
1.00
23.25


ATOM
2669
CB
THR
A
346
−26.246
6.338
1.101
1.00
23.78


ATOM
2670
OG1
THR
A
346
−27.327
5.396
1.020
1.00
27.42


ATOM
2671
CG2
THR
A
346
−25.507
6.129
2.443
1.00
24.74


ATOM
2672
C
THR
A
346
−25.753
8.849
1.138
1.00
23.03


ATOM
2673
O
THR
A
346
−24.916
9.067
0.260
1.00
23.47


ATOM
2674
N
VAL
A
347
−25.848
9.561
2.255
1.00
21.33


ATOM
2675
CA
VAL
A
347
−24.845
10.537
2.674
1.00
20.11


ATOM
2676
CB
VAL
A
347
−25.522
11.844
3.212
1.00
19.06


ATOM
2677
CG1
VAL
A
347
−24.489
12.834
3.700
1.00
19.66


ATOM
2678
CG2
VAL
A
347
−26.418
12.465
2.137
1.00
20.32


ATOM
2679
C
VAL
A
347
−24.066
9.865
3.785
1.00
20.14


ATOM
2680
O
VAL
A
347
−24.667
9.340
4.728
1.00
19.79


ATOM
2681
N
THR
A
348
−22.734
9.878
3.671
1.00
19.85


ATOM
2682
CA
THR
A
348
−21.851
9.274
4.660
1.00
19.92


ATOM
2683
CB
THR
A
348
−20.965
8.185
4.018
1.00
19.82


ATOM
2684
OG1
THR
A
348
−19.921
8.815
3.277
1.00
20.35


ATOM
2685
CG2
THR
A
348
−21.785
7.278
3.092
1.00
21.67


ATOM
2686
C
THR
A
348
−20.964
10.354
5.256
1.00
19.60


ATOM
2687
O
THR
A
348
−20.961
11.484
4.760
1.00
19.51


ATOM
2688
N
ALA
A
349
−20.191
10.006
6.292
1.00
19.18


ATOM
2689
CA
ALA
A
349
−19.243
10.932
6.885
1.00
20.02


ATOM
2690
CB
ALA
A
349
−18.494
10.275
8.044
1.00
20.61


ATOM
2691
C
ALA
A
349
−18.240
11.466
5.842
1.00
19.79


ATOM
2692
O
ALA
A
349
−17.756
12.601
5.947
1.00
20.15


ATOM
2693
N
THR
A
350
−17.906
10.619
4.873
1.00
18.87


ATOM
2694
CA
THR
A
350
−16.911
10.971
3.850
1.00
18.30


ATOM
2695
CB
THR
A
350
−16.435
9.717
3.093
1.00
18.67


ATOM
2696
OG1
THR
A
350
−15.780
8.850
4.027
1.00
19.82


ATOM
2697
CG2
THR
A
350
−15.426
10.097
1.974
1.00
17.73


ATOM
2698
C
THR
A
350
−17.463
12.003
2.871
1.00
17.30


ATOM
2699
O
THR
A
350
−16.747
12.930
2.487
1.00
17.99


ATOM
2700
N
SER
A
351
−18.716
11.847
2.467
1.00
16.16


ATOM
2701
CA
SER
A
351
−19.316
12.803
1.517
1.00
15.78


ATOM
2702
CB
SER
A
351
−20.214
12.076
0.512
1.00
15.85


ATOM
2703
OG
SER
A
351
−21.280
11.412
1.156
1.00
17.14


ATOM
2704
C
SER
A
351
−20.087
13.941
2.193
1.00
15.63


ATOM
2705
O
SER
A
351
−20.736
14.743
1.524
1.00
13.62


ATOM
2706
N
LEU
A
352
−20.048
14.006
3.527
1.00
15.09


ATOM
2707
CA
LEU
A
352
−20.901
14.985
4.212
1.00
16.29


ATOM
2708
CB
LEU
A
352
−20.759
14.851
5.736
1.00
16.64


ATOM
2709
CG
LEU
A
352
−21.713
15.734
6.570
1.00
17.22


ATOM
2710
CD1
LEU
A
352
−23.138
15.281
6.418
1.00
19.06


ATOM
2711
CD2
LEU
A
352
−21.263
15.636
8.032
1.00
20.02


ATOM
2712
C
LEU
A
352
−20.592
16.427
3.787
1.00
16.09


ATOM
2713
O
LEU
A
352
−21.499
17.219
3.601
1.00
16.35


ATOM
2714
N
ALA
A
353
−19.311
16.763
3.643
1.00
16.19


ATOM
2715
CA
ALA
A
353
−18.933
18.148
3.354
1.00
15.74


ATOM
2716
CB
ALA
A
353
−17.460
18.314
3.424
1.00
16.30


ATOM
2717
C
ALA
A
353
−19.459
18.544
1.972
1.00
16.11


ATOM
2718
O
ALA
A
353
−19.957
19.668
1.781
1.00
15.44


ATOM
2719
N
PHE
A
354
−19.367
17.607
1.021
1.00
16.10


ATOM
2720
CA
PHE
A
354
−19.885
17.849
−0.325
1.00
15.25


ATOM
2721
CB
PHE
A
354
−19.718
16.618
−1.220
1.00
16.59


ATOM
2722
CG
PHE
A
354
−20.497
16.707
−2.500
1.00
15.45


ATOM
2723
CD1
PHE
A
354
−19.959
17.375
−3.603
1.00
16.95


ATOM
2724
CE1
PHE
A
354
−20.664
17.489
−4.793
1.00
17.06


ATOM
2725
CZ
PHE
A
354
−21.956
16.953
−4.888
1.00
16.75


ATOM
2726
CE2
PHE
A
354
−22.517
16.276
−3.791
1.00
16.91


ATOM
2727
CD2
PHE
A
354
−21.778
16.160
−2.594
1.00
17.48


ATOM
2728
C
PHE
A
354
−21.374
18.188
−0.226
1.00
15.56


ATOM
2729
O
PHE
A
354
−21.815
19.183
−0.797
1.00
15.08


ATOM
2730
N
PHE
A
355
−22.140
17.347
0.474
1.00
14.54


ATOM
2731
CA
PHE
A
355
−23.588
17.544
0.517
1.00
15.29


ATOM
2732
CB
PHE
A
355
−24.295
16.319
1.078
1.00
15.61


ATOM
2733
CG
PHE
A
355
−24.386
15.176
0.112
1.00
15.51


ATOM
2734
CD1
PHE
A
355
−25.306
15.205
−0.945
1.00
14.91


ATOM
2735
CE1
PHE
A
355
−25.404
14.131
−1.832
1.00
16.52


ATOM
2736
CZ
PHE
A
355
−24.567
13.033
−1.676
1.00
16.12


ATOM
2737
CE2
PHE
A
355
−23.648
12.994
−0.628
1.00
15.62


ATOM
2738
CD2
PHE
A
355
−23.562
14.071
0.255
1.00
13.62


ATOM
2739
C
PHE
A
355
−23.988
18.789
1.303
1.00
15.19


ATOM
2740
O
PHE
A
355
−24.920
19.477
0.902
1.00
15.69


ATOM
2741
N
GLN
A
356
−23.283
19.084
2.398
1.00
15.78


ATOM
2742
CA
GLN
A
356
−23.679
20.216
3.257
1.00
16.16


ATOM
2743
CB
GLN
A
356
−22.987
20.165
4.627
1.00
16.84


ATOM
2744
CG
GLN
A
356
−23.564
19.115
5.579
1.00
17.13


ATOM
2745
CD
GLN
A
356
−22.907
19.170
6.973
1.00
18.06


ATOM
2746
OE1
GLN
A
356
−21.808
19.707
7.138
1.00
20.29


ATOM
2747
NE2
GLN
A
356
−23.569
18.589
7.965
1.00
20.95


ATOM
2748
C
GLN
A
356
−23.444
21.546
2.584
1.00
16.08


ATOM
2749
O
GLN
A
356
−24.142
22.507
2.868
1.00
15.59


ATOM
2750
N
GLU
A
357
−22.482
21.599
1.661
1.00
16.07


ATOM
2751
CA
GLU
A
357
−22.261
22.808
0.884
1.00
16.41


ATOM
2752
CB
GLU
A
357
−20.994
22.683
0.005
1.00
16.33


ATOM
2753
CG
GLU
A
357
−20.671
23.942
−0.770
1.00
15.64


ATOM
2754
CD
GLU
A
357
−19.326
23.894
−1.516
1.00
17.67


ATOM
2755
OE1
GLU
A
357
−18.931
24.947
−2.066
1.00
19.03


ATOM
2756
OE2
GLU
A
357
−18.685
22.822
−1.575
1.00
14.75


ATOM
2757
C
GLU
A
357
−23.492
23.105
0.019
1.00
15.84


ATOM
2758
O
GLU
A
357
−23.786
24.237
−0.224
1.00
18.07


ATOM
2759
N
LEU
A
358
−24.213
22.084
−0.420
1.00
14.72


ATOM
2760
CA
LEU
A
358
−25.364
22.251
−1.310
1.00
15.75


ATOM
2761
CB
LEU
A
358
−25.368
21.147
−2.369
1.00
16.49


ATOM
2762
CG
LEU
A
358
−24.057
21.100
−3.168
1.00
16.81


ATOM
2763
CD1
LEU
A
358
−24.087
19.977
−4.182
1.00
19.73


ATOM
2764
CD2
LEU
A
358
−23.775
22.465
−3.846
1.00
19.34


ATOM
2765
C
LEU
A
358
−26.708
22.251
−0.582
1.00
15.36


ATOM
2766
O
LEU
A
358
−27.656
22.911
−1.028
1.00
14.78


ATOM
2767
N
VAL
A
359
−26.786
21.511
0.520
1.00
15.34


ATOM
2768
CA
VAL
A
359
−28.001
21.404
1.321
1.00
15.00


ATOM
2769
CB
VAL
A
359
−28.691
20.006
1.154
1.00
15.90


ATOM
2770
CG1
VAL
A
359
−29.999
19.917
1.962
1.00
15.16


ATOM
2771
CG2
VAL
A
359
−28.967
19.685
−0.348
1.00
16.26


ATOM
2772
C
VAL
A
359
−27.531
21.624
2.775
1.00
14.72


ATOM
2773
O
VAL
A
359
−27.192
20.653
3.500
1.00
14.22


ATOM
2774
N
PRO
A
360
−27.467
22.893
3.193
1.00
14.98


ATOM
2775
CA
PRO
A
360
−26.937
23.179
4.539
1.00
15.45


ATOM
2776
CB
PRO
A
360
−27.150
24.700
4.700
1.00
15.45


ATOM
2777
CG
PRO
A
360
−27.188
25.219
3.274
1.00
16.15


ATOM
2778
CD
PRO
A
360
−27.854
24.127
2.471
1.00
14.54


ATOM
2779
C
PRO
A
360
−27.692
22.385
5.611
1.00
15.31


ATOM
2780
O
PRO
A
360
−28.918
22.262
5.555
1.00
15.07


ATOM
2781
N
GLY
A
361
−26.936
21.842
6.560
1.00
15.94


ATOM
2782
CA
GLY
A
361
−27.512
21.143
7.709
1.00
16.53


ATOM
2783
C
GLY
A
361
−27.870
19.680
7.488
1.00
17.03


ATOM
2784
O
GLY
A
361
−28.268
18.997
8.429
1.00
17.68


ATOM
2785
N
VAL
A
362
−27.762
19.176
6.261
1.00
16.13


ATOM
2786
CA
VAL
A
362
−28.163
17.769
6.037
1.00
16.72


ATOM
2787
CB
VAL
A
362
−28.217
17.416
4.525
1.00
16.47


ATOM
2788
CG1
VAL
A
362
−26.808
17.311
3.947
1.00
16.75


ATOM
2789
CG2
VAL
A
362
−29.054
16.142
4.280
1.00
17.21


ATOM
2790
C
VAL
A
362
−27.208
16.849
6.811
1.00
17.24


ATOM
2791
O
VAL
A
362
−26.044
17.187
7.006
1.00
17.07


ATOM
2792
N
THR
A
363
−27.695
15.703
7.274
1.00
18.15


ATOM
2793
CA
THR
A
363
−26.821
14.789
8.025
1.00
19.86


ATOM
2794
CB
THR
A
363
−27.388
14.459
9.405
1.00
20.59


ATOM
2795
OG1
THR
A
363
−28.634
13.776
9.217
1.00
22.85


ATOM
2796
CG2
THR
A
363
−27.610
15.742
10.182
1.00
22.71


ATOM
2797
C
THR
A
363
−26.660
13.476
7.310
1.00
19.18


ATOM
2798
O
THR
A
363
−27.398
13.184
6.371
1.00
18.87


ATOM
2799
N
ALA
A
364
−25.697
12.679
7.769
1.00
19.67


ATOM
2800
CA
ALA
A
364
−25.495
11.342
7.222
1.00
20.19


ATOM
2801
CB
ALA
A
364
−24.361
10.637
7.930
1.00
20.38


ATOM
2802
C
ALA
A
364
−26.783
10.541
7.343
1.00
21.17


ATOM
2803
O
ALA
A
364
−27.551
10.720
8.293
1.00
21.92


ATOM
2804
N
GLY
A
365
−27.041
9.687
6.360
1.00
21.67


ATOM
2805
CA
GLY
A
365
−28.207
8.823
6.371
1.00
22.35


ATOM
2806
C
GLY
A
365
−28.584
8.415
4.968
1.00
23.66


ATOM
2807
O
GLY
A
365
−27.924
8.804
3.991
1.00
23.66


ATOM
2808
N
THR
A
366
−29.639
7.615
4.862
1.00
23.70


ATOM
2809
CA
THR
A
366
−30.148
7.183
3.582
1.00
24.56


ATOM
2810
CB
THR
A
366
−30.188
5.644
3.491
1.00
25.67


ATOM
2811
OG1
THR
A
366
−28.849
5.143
3.649
1.00
27.09


ATOM
2812
CG2
THR
A
366
−30.715
5.216
2.159
1.00
25.47


ATOM
2813
C
THR
A
366
−31.520
7.769
3.344
1.00
25.15


ATOM
2814
O
THR
A
366
−32.427
7.612
4.177
1.00
25.01


ATOM
2815
N
TYR
A
367
−31.668
8.447
2.210
1.00
24.20


ATOM
2816
CA
TYR
A
367
−32.900
9.146
1.883
1.00
24.30


ATOM
2817
CB
TYR
A
367
−32.616
10.648
1.701
1.00
23.51


ATOM
2818
CG
TYR
A
367
−31.924
11.238
2.907
1.00
22.68


ATOM
2819
CD1
TYR
A
367
−32.639
11.506
4.078
1.00
21.99


ATOM
2820
CE1
TYR
A
367
−32.012
12.019
5.199
1.00
20.27


ATOM
2821
CZ
TYR
A
367
−30.650
12.263
5.176
1.00
22.67


ATOM
2822
OH
TYR
A
367
−30.036
12.789
6.287
1.00
21.24


ATOM
2823
CE2
TYR
A
367
−29.897
11.994
4.023
1.00
20.71


ATOM
2824
CD2
TYR
A
367
−30.541
11.479
2.904
1.00
20.37


ATOM
2825
C
TYR
A
367
−33.531
8.542
0.641
1.00
25.39


ATOM
2826
O
TYR
A
367
−32.900
8.456
−0.415
1.00
24.86


ATOM
2827
N
SER
A
368
−34.782
8.109
0.758
1.00
25.82


ATOM
2828
CA
SER
A
368
−35.416
7.454
−0.374
1.00
27.29


ATOM
2829
CB
SER
A
368
−36.339
6.332
0.107
1.00
27.90


ATOM
2830
OG
SER
A
368
−37.519
6.895
0.634
1.00
30.69


ATOM
2831
C
SER
A
368
−36.171
8.466
−1.218
1.00
27.68


ATOM
2832
O
SER
A
368
−36.361
9.612
−0.805
1.00
26.96


ATOM
2833
N
SER
A
369
−36.629
8.025
−2.388
1.00
28.98


ATOM
2834
CA
SER
A
369
−37.260
8.908
−3.367
1.00
30.52


ATOM
2835
CB
SER
A
369
−37.520
8.167
−4.681
1.00
30.90


ATOM
2836
OG
SER
A
369
−38.269
6.983
−4.452
1.00
32.57


ATOM
2837
C
SER
A
369
−38.536
9.583
−2.871
1.00
31.50


ATOM
2838
O
SER
A
369
−38.954
10.572
−3.442
1.00
32.33


ATOM
2839
N
SER
A
370
−39.150
9.067
−1.809
1.00
32.18


ATOM
2840
CA
SER
A
370
−40.365
9.712
−1.279
1.00
32.96


ATOM
2841
CB
SER
A
370
−41.313
8.692
−0.624
1.00
33.18


ATOM
2842
OG
SER
A
370
−40.610
7.847
0.273
1.00
34.18


ATOM
2843
C
SER
A
370
−40.049
10.864
−0.323
1.00
32.37


ATOM
2844
O
SER
A
370
−40.901
11.729
−0.078
1.00
33.26


ATOM
2845
N
SER
A
371
−38.825
10.893
0.197
1.00
31.13


ATOM
2846
CA
SER
A
371
−38.443
11.911
1.174
1.00
30.04


ATOM
2847
CB
SER
A
371
−37.180
11.487
1.912
1.00
29.83


ATOM
2848
OG
SER
A
371
−36.046
11.714
1.100
1.00
30.43


ATOM
2849
C
SER
A
371
−38.247
13.295
0.553
1.00
29.13


ATOM
2850
O
SER
A
371
−37.795
13.424
−0.589
1.00
28.84


ATOM
2851
N
SER
A
372
−38.571
14.340
1.312
1.00
27.84


ATOM
2852
CA
SER
A
372
−38.300
15.689
0.845
1.00
27.18


ATOM
2853
CB
SER
A
372
−38.896
16.737
1.789
1.00
27.36


ATOM
2854
OG
SER
A
372
−38.331
16.609
3.080
1.00
28.50


ATOM
2855
C
SER
A
372
−36.783
15.902
0.680
1.00
25.79


ATOM
2856
O
SER
A
372
−36.358
16.690
−0.173
1.00
26.29


ATOM
2857
N
THR
A
373
−35.979
15.193
1.479
1.00
24.06


ATOM
2858
CA
THR
A
373
−34.517
15.337
1.448
1.00
22.51


ATOM
2859
CB
THR
A
373
−33.833
14.501
2.545
1.00
22.26


ATOM
2860
OG1
THR
A
373
−34.543
14.636
3.788
1.00
23.04


ATOM
2861
CG2
THR
A
373
−32.370
14.926
2.734
1.00
21.06


ATOM
2862
C
THR
A
373
−33.984
14.906
0.076
1.00
21.61


ATOM
2863
O
THR
A
373
−33.134
15.578
−0.513
1.00
19.74


ATOM
2864
N
PHE
A
374
−34.493
13.777
−0.413
1.00
21.13


ATOM
2865
CA
PHE
A
374
−34.137
13.280
−1.749
1.00
22.10


ATOM
2866
CB
PHE
A
374
−34.950
12.023
−2.051
1.00
22.12


ATOM
2867
CG
PHE
A
374
−34.624
11.380
−3.366
1.00
22.90


ATOM
2868
CD1
PHE
A
374
−33.677
10.368
−3.432
1.00
23.83


ATOM
2869
CE1
PHE
A
374
−33.381
9.749
−4.649
1.00
22.56


ATOM
2870
CZ
PHE
A
374
−34.041
10.162
−5.802
1.00
23.24


ATOM
2871
CE2
PHE
A
374
−34.985
11.161
−5.752
1.00
23.36


ATOM
2872
CD2
PHE
A
374
−35.280
11.769
−4.523
1.00
23.73


ATOM
2873
C
PHE
A
374
−34.343
14.349
−2.818
1.00
22.24


ATOM
2874
O
PHE
A
374
−33.413
14.681
−3.548
1.00
22.63


ATOM
2875
N
THR
A
375
−35.549
14.923
−2.880
1.00
22.72


ATOM
2876
CA
THR
A
375
−35.890
15.963
−3.852
1.00
23.34


ATOM
2877
CB
THR
A
375
−37.364
16.398
−3.683
1.00
23.63


ATOM
2878
OG1
THR
A
375
−38.193
15.244
−3.809
1.00
27.83


ATOM
2879
CG2
THR
A
375
−37.768
17.413
−4.749
1.00
27.05


ATOM
2880
C
THR
A
375
−35.003
17.203
−3.746
1.00
22.66


ATOM
2881
O
THR
A
375
−34.603
17.766
−4.756
1.00
21.43


ATOM
2882
N
ASN
A
376
−34.744
17.632
−2.508
1.00
20.80


ATOM
2883
CA
ASN
A
376
−33.880
18.766
−2.207
1.00
21.33


ATOM
2884
CB
ASN
A
376
−33.856
18.975
−0.688
1.00
21.98


ATOM
2885
CG
ASN
A
376
−33.343
20.354
−0.278
1.00
27.01


ATOM
2886
OD1
ASN
A
376
−32.582
21.011
−1.004
1.00
31.72


ATOM
2887
ND2
ASN
A
376
−33.748
20.793
0.913
1.00
30.14


ATOM
2888
C
ASN
A
376
−32.465
18.527
−2.733
1.00
19.80


ATOM
2889
O
ASN
A
376
−31.898
19.389
−3.415
1.00
19.75


ATOM
2890
N
ILE
A
377
−31.915
17.354
−2.431
1.00
19.00


ATOM
2891
CA
ILE
A
377
−30.586
16.983
−2.916
1.00
18.66


ATOM
2892
CB
ILE
A
377
−30.081
15.651
−2.319
1.00
18.56


ATOM
2893
CG1
ILE
A
377
−29.834
15.813
−0.800
1.00
18.42


ATOM
2894
CD1
ILE
A
377
−29.634
14.481
−0.028
1.00
19.35


ATOM
2895
CG2
ILE
A
377
−28.787
15.233
−3.025
1.00
18.07


ATOM
2896
C
ILE
A
377
−30.546
16.964
−4.451
1.00
18.87


ATOM
2897
O
ILE
A
377
−29.655
17.575
−5.058
1.00
18.47


ATOM
2898
N
ILE
A
378
−31.513
16.293
−5.068
1.00
18.45


ATOM
2899
CA
ILE
A
378
−31.556
16.216
−6.539
1.00
19.78


ATOM
2900
CB
ILE
A
378
−32.738
15.359
−7.085
1.00
20.45


ATOM
2901
CG1
ILE
A
378
−32.593
13.891
−6.650
1.00
22.32


ATOM
2902
CD1
ILE
A
378
−31.414
13.145
−7.270
1.00
24.68


ATOM
2903
CG2
ILE
A
378
−32.829
15.472
−8.646
1.00
21.27


ATOM
2904
C
ILE
A
378
−31.561
17.588
−7.177
1.00
19.82


ATOM
2905
O
ILE
A
378
−30.760
17.849
−8.101
1.00
19.62


ATOM
2906
N
ASN
A
379
−32.441
18.470
−6.689
1.00
18.68


ATOM
2907
CA
ASN
A
379
−32.531
19.820
−7.224
1.00
19.13


ATOM
2908
CB
ASN
A
379
−33.738
20.578
−6.658
1.00
20.08


ATOM
2909
CG
ASN
A
379
−35.066
19.956
−7.087
1.00
25.11


ATOM
2910
OD1
ASN
A
379
−35.121
19.173
−8.044
1.00
29.37


ATOM
2911
ND2
ASN
A
379
−36.144
20.289
−6.369
1.00
29.08


ATOM
2912
C
ASN
A
379
−31.241
20.604
−7.040
1.00
17.96


ATOM
2913
O
ASN
A
379
−30.774
21.273
−7.981
1.00
17.85


ATOM
2914
N
ALA
A
380
−30.662
20.497
−5.841
1.00
15.93


ATOM
2915
CA
ALA
A
380
−29.458
21.241
−5.509
1.00
16.08


ATOM
2916
CB
ALA
A
380
−29.120
21.061
−4.033
1.00
16.14


ATOM
2917
C
ALA
A
380
−28.299
20.783
−6.389
1.00
15.64


ATOM
2918
O
ALA
A
380
−27.566
21.607
−6.938
1.00
16.56


ATOM
2919
N
VAL
A
381
−28.153
19.471
−6.519
1.00
15.22


ATOM
2920
CA
VAL
A
381
−27.039
18.912
−7.302
1.00
15.77


ATOM
2921
CB
VAL
A
381
−26.823
17.403
−6.999
1.00
15.61


ATOM
2922
CG1
VAL
A
381
−25.747
16.777
−7.940
1.00
14.83


ATOM
2923
CG2
VAL
A
381
−26.386
17.234
−5.551
1.00
14.84


ATOM
2924
C
VAL
A
381
−27.243
19.211
−8.794
1.00
16.08


ATOM
2925
O
VAL
A
381
−26.281
19.508
−9.505
1.00
16.62


ATOM
2926
N
SER
A
382
−28.482
19.112
−9.278
1.00
16.66


ATOM
2927
CA
SER
A
382
−28.772
19.453
−10.690
1.00
18.67


ATOM
2928
CB
SER
A
382
−30.246
19.212
−11.043
1.00
18.26


ATOM
2929
OG
SER
A
382
−30.538
17.855
−10.893
1.00
24.56


ATOM
2930
C
SER
A
382
−28.434
20.894
−11.005
1.00
18.10


ATOM
2931
O
SER
A
382
−27.815
21.183
−12.027
1.00
18.05


ATOM
2932
N
THR
A
383
−28.853
21.810
−10.132
1.00
17.66


ATOM
2933
CA
THR
A
383
−28.521
23.216
−10.298
1.00
17.72


ATOM
2934
CB
THR
A
383
−29.199
24.063
−9.180
1.00
18.48


ATOM
2935
OG1
THR
A
383
−30.606
23.985
−9.373
1.00
19.72


ATOM
2936
CG2
THR
A
383
−28.771
25.550
−9.227
1.00
19.59


ATOM
2937
C
THR
A
383
−27.017
23.470
−10.314
1.00
17.09


ATOM
2938
O
THR
A
383
−26.524
24.286
−11.109
1.00
17.00


ATOM
2939
N
TYR
A
384
−26.299
22.774
−9.435
1.00
15.62


ATOM
2940
CA
TYR
A
384
−24.858
22.925
−9.312
1.00
15.37


ATOM
2941
CB
TYR
A
384
−24.397
22.164
−8.068
1.00
15.00


ATOM
2942
CG
TYR
A
384
−22.958
22.345
−7.630
1.00
15.08


ATOM
2943
CD1
TYR
A
384
−22.361
23.601
−7.578
1.00
15.83


ATOM
2944
CE1
TYR
A
384
−21.049
23.752
−7.131
1.00
16.33


ATOM
2945
CZ
TYR
A
384
−20.321
22.623
−6.737
1.00
16.13


ATOM
2946
OH
TYR
A
384
−19.018
22.738
−6.302
1.00
16.22


ATOM
2947
CE2
TYR
A
384
−20.890
21.386
−6.778
1.00
13.72


ATOM
2948
CD2
TYR
A
384
−22.203
21.242
−7.232
1.00
14.72


ATOM
2949
C
TYR
A
384
−24.186
22.396
−10.590
1.00
15.13


ATOM
2950
O
TYR
A
384
−23.319
23.065
−11.173
1.00
14.43


ATOM
2951
N
ALA
A
385
−24.605
21.213
−11.033
1.00
15.02


ATOM
2952
CA
ALA
A
385
−24.045
20.639
−12.263
1.00
15.60


ATOM
2953
CB
ALA
A
385
−24.634
19.295
−12.503
1.00
15.85


ATOM
2954
C
ALA
A
385
−24.249
21.564
−13.477
1.00
16.15


ATOM
2955
O
ALA
A
385
−23.292
21.857
−14.211
1.00
15.49


ATOM
2956
N
ASP
A
386
−25.483
22.055
−13.660
1.00
15.57


ATOM
2957
CA
ASP
A
386
−25.782
23.058
−14.694
1.00
16.09


ATOM
2958
CB
ASP
A
386
−27.279
23.433
−14.687
1.00
15.88


ATOM
2959
CG
ASP
A
386
−28.158
22.379
−15.349
1.00
18.85


ATOM
2960
OD1
ASP
A
386
−27.672
21.307
−15.766
1.00
18.94


ATOM
2961
OD2
ASP
A
386
−29.365
22.624
−15.461
1.00
23.88


ATOM
2962
C
ASP
A
386
−24.938
24.322
−14.526
1.00
15.81


ATOM
2963
O
ASP
A
386
−24.594
24.998
−15.501
1.00
16.29


ATOM
2964
N
GLY
A
387
−24.591
24.640
−13.290
1.00
15.76


ATOM
2965
CA
GLY
A
387
−23.735
25.787
−13.038
1.00
14.35


ATOM
2966
C
GLY
A
387
−22.354
25.654
−13.663
1.00
14.35


ATOM
2967
O
GLY
A
387
−21.791
26.644
−14.129
1.00
13.79


ATOM
2968
N
PHE
A
388
−21.771
24.453
−13.624
1.00
14.30


ATOM
2969
CA
PHE
A
388
−20.479
24.217
−14.312
1.00
14.69


ATOM
2970
CB
PHE
A
388
−19.912
22.824
−13.987
1.00
14.42


ATOM
2971
CG
PHE
A
388
−19.359
22.730
−12.584
1.00
13.79


ATOM
2972
CD1
PHE
A
388
−18.139
23.335
−12.269
1.00
14.50


ATOM
2973
CE1
PHE
A
388
−17.621
23.261
−10.947
1.00
16.38


ATOM
2974
CZ
PHE
A
388
−18.377
22.627
−9.951
1.00
14.77


ATOM
2975
CE2
PHE
A
388
−19.604
22.045
−10.265
1.00
16.35


ATOM
2976
CD2
PHE
A
388
−20.088
22.100
−11.578
1.00
14.00


ATOM
2977
C
PHE
A
388
−20.601
24.428
−15.821
1.00
15.31


ATOM
2978
O
PHE
A
388
−19.740
25.078
−16.440
1.00
15.55


ATOM
2979
N
LEU
A
389
−21.669
23.913
−16.415
1.00
16.52


ATOM
2980
CA
LEU
A
389
−21.889
24.159
−17.856
1.00
17.84


ATOM
2981
CB
LEU
A
389
−23.137
23.431
−18.382
1.00
17.83


ATOM
2982
CG
LEU
A
389
−23.172
21.911
−18.427
1.00
22.75


ATOM
2983
CD1
LEU
A
389
−24.247
21.418
−19.401
1.00
22.55


ATOM
2984
CD2
LEU
A
389
−21.805
21.333
−18.806
1.00
24.63


ATOM
2985
C
LEU
A
389
−22.013
25.634
−18.136
1.00
18.32


ATOM
2986
O
LEU
A
389
−21.409
26.138
−19.091
1.00
19.50


ATOM
2987
N
SER
A
390
−22.775
26.341
−17.295
1.00
18.55


ATOM
2988
CA
SER
A
390
−23.021
27.767
−17.469
1.00
19.33


ATOM
2989
CB
SER
A
390
−24.090
28.246
−16.491
1.00
19.89


ATOM
2990
OG
SER
A
390
−25.325
27.693
−16.891
1.00
24.07


ATOM
2991
C
SER
A
390
−21.763
28.603
−17.323
1.00
19.51


ATOM
2992
O
SER
A
390
−21.575
29.585
−18.055
1.00
19.69


ATOM
2993
N
GLU
A
391
−20.893
28.200
−16.399
1.00
18.87


ATOM
2994
CA
GLU
A
391
−19.633
28.879
−16.220
1.00
19.59


ATOM
2995
CB
GLU
A
391
−18.901
28.393
−14.952
1.00
20.02


ATOM
2996
CG
GLU
A
391
−19.528
28.924
−13.668
1.00
23.54


ATOM
2997
CD
GLU
A
391
−19.590
30.448
−13.634
1.00
26.61


ATOM
2998
OE1
GLU
A
391
−18.609
31.102
−14.023
1.00
28.42


ATOM
2999
OE2
GLU
A
391
−20.637
30.994
−13.227
1.00
29.52


ATOM
3000
C
GLU
A
391
−18.738
28.729
−17.457
1.00
19.12


ATOM
3001
O
GLU
A
391
−18.123
29.709
−17.906
1.00
19.40


ATOM
3002
N
ALA
A
392
−18.654
27.516
−17.991
1.00
18.81


ATOM
3003
CA
ALA
A
392
−17.861
27.304
−19.201
1.00
19.72


ATOM
3004
CB
ALA
A
392
−17.758
25.815
−19.526
1.00
19.45


ATOM
3005
C
ALA
A
392
−18.478
28.098
−20.363
1.00
19.59


ATOM
3006
O
ALA
A
392
−17.764
28.724
−21.157
1.00
18.65


ATOM
3007
N
ALA
A
393
−19.808
28.117
−20.425
1.00
20.40


ATOM
3008
CA
ALA
A
393
−20.526
28.820
−21.507
1.00
21.27


ATOM
3009
CB
ALA
A
393
−22.035
28.524
−21.421
1.00
21.83


ATOM
3010
C
ALA
A
393
−20.266
30.334
−21.574
1.00
21.79


ATOM
3011
O
ALA
A
393
−20.283
30.920
−22.664
1.00
21.96


ATOM
3012
N
LYS
A
394
−19.976
30.971
−20.435
1.00
21.37


ATOM
3013
CA
LYS
A
394
−19.626
32.383
−20.447
1.00
22.02


ATOM
3014
CB
LYS
A
394
−19.289
32.916
−19.043
1.00
23.11


ATOM
3015
CG
LYS
A
394
−20.411
32.980
−18.044
1.00
25.98


ATOM
3016
CD
LYS
A
394
−19.782
33.341
−16.700
1.00
28.89


ATOM
3017
CE
LYS
A
394
−20.793
33.314
−15.576
1.00
34.05


ATOM
3018
NZ
LYS
A
394
−20.097
33.674
−14.290
1.00
33.96


ATOM
3019
C
LYS
A
394
−18.403
32.626
−21.310
1.00
21.55


ATOM
3020
O
LYS
A
394
−18.188
33.742
−21.771
1.00
22.41


ATOM
3021
N
TYR
A
395
−17.570
31.604
−21.488
1.00
19.98


ATOM
3022
CA
TYR
A
395
−16.287
31.824
−22.132
1.00
19.46


ATOM
3023
CB
TYR
A
395
−15.137
31.464
−21.185
1.00
20.82


ATOM
3024
CG
TYR
A
395
−15.291
32.165
−19.872
1.00
22.04


ATOM
3025
CD1
TYR
A
395
−15.644
31.450
−18.716
1.00
23.21


ATOM
3026
CE1
TYR
A
395
−15.806
32.097
−17.508
1.00
23.70


ATOM
3027
CZ
TYR
A
395
−15.661
33.473
−17.460
1.00
23.93


ATOM
3028
OH
TYR
A
395
−15.828
34.143
−16.272
1.00
26.14


ATOM
3029
CE2
TYR
A
395
−15.327
34.202
−18.593
1.00
24.38


ATOM
3030
CD2
TYR
A
395
−15.157
33.548
−19.791
1.00
22.37


ATOM
3031
C
TYR
A
395
−16.157
31.119
−23.451
1.00
19.22


ATOM
3032
O
TYR
A
395
−15.045
30.940
−23.941
1.00
18.70


ATOM
3033
N
VAL
A
396
−17.299
30.718
−24.018
1.00
18.16


ATOM
3034
CA
VAL
A
396
−17.331
30.135
−25.352
1.00
19.03


ATOM
3035
CB
VAL
A
396
−18.396
29.025
−25.458
1.00
18.18


ATOM
3036
CG1
VAL
A
396
−18.469
28.465
−26.898
1.00
18.63


ATOM
3037
CG2
VAL
A
396
−18.094
27.915
−24.452
1.00
18.97


ATOM
3038
C
VAL
A
396
−17.654
31.288
−26.308
1.00
19.65


ATOM
3039
O
VAL
A
396
−18.644
31.986
−26.098
1.00
19.66


ATOM
3040
N
PRO
A
397
−16.810
31.507
−27.328
1.00
20.41


ATOM
3041
CA
PRO
A
397
−17.016
32.626
−28.256
1.00
20.82


ATOM
3042
CB
PRO
A
397
−15.794
32.561
−29.175
1.00
21.47


ATOM
3043
CG
PRO
A
397
−14.819
31.725
−28.475
1.00
21.69


ATOM
3044
CD
PRO
A
397
−15.598
30.741
−27.661
1.00
19.70


ATOM
3045
C
PRO
A
397
−18.280
32.434
−29.073
1.00
21.11


ATOM
3046
O
PRO
A
397
−18.844
31.339
−29.088
1.00
19.88


ATOM
3047
N
ALA
A
398
−18.713
33.492
−29.765
1.00
21.26


ATOM
3048
CA
ALA
A
398
−19.951
33.424
−30.559
1.00
21.44


ATOM
3049
CB
ALA
A
398
−20.227
34.766
−31.230
1.00
22.38


ATOM
3050
C
ALA
A
398
−19.971
32.297
−31.587
1.00
21.30


ATOM
3051
O
ALA
A
398
−21.038
31.769
−31.901
1.00
22.15


ATOM
3052
N
ASP
A
399
−18.804
31.896
−32.102
1.00
20.30


ATOM
3053
CA
ASP
A
399
−18.780
30.858
−33.133
1.00
19.40


ATOM
3054
CB
ASP
A
399
−17.587
31.032
−34.071
1.00
19.42


ATOM
3055
CG
ASP
A
399
−16.233
30.835
−33.381
1.00
21.84


ATOM
3056
OD1
ASP
A
399
−16.146
30.569
−32.159
1.00
20.91


ATOM
3057
OD2
ASP
A
399
−15.229
30.950
−34.104
1.00
24.62


ATOM
3058
C
ASP
A
399
−18.834
29.435
−32.579
1.00
18.14


ATOM
3059
O
ASP
A
399
−18.802
28.465
−33.350
1.00
16.78


ATOM
3060
N
GLY
A
400
−18.891
29.322
−31.245
1.00
16.82


ATOM
3061
CA
GLY
A
400
−18.996
28.015
−30.607
1.00
15.41


ATOM
3062
C
GLY
A
400
−17.693
27.229
−30.556
1.00
15.07


ATOM
3063
O
GLY
A
400
−17.704
26.041
−30.203
1.00
15.23


ATOM
3064
N
SER
A
401
−16.572
27.861
−30.882
1.00
14.21


ATOM
3065
CA
SER
A
401
−15.312
27.119
−30.893
1.00
14.76


ATOM
3066
CB
SER
A
401
−14.241
27.840
−31.718
1.00
14.71


ATOM
3067
OG
SER
A
401
−14.059
29.160
−31.257
1.00
16.86


ATOM
3068
C
SER
A
401
−14.815
26.866
−29.448
1.00
14.38


ATOM
3069
O
SER
A
401
−14.992
27.717
−28.562
1.00
14.58


ATOM
3070
N
LEU
A
402
−14.169
25.720
−29.249
1.00
13.54


ATOM
3071
CA
LEU
A
402
−13.603
25.364
−27.968
1.00
13.21


ATOM
3072
CB
LEU
A
402
−14.271
24.080
−27.450
1.00
13.42


ATOM
3073
CG
LEU
A
402
−15.776
24.192
−27.162
1.00
14.06


ATOM
3074
CD1
LEU
A
402
−16.289
22.834
−26.668
1.00
13.87


ATOM
3075
CD2
LEU
A
402
−15.997
25.264
−26.109
1.00
17.00


ATOM
3076
C
LEU
A
402
−12.111
25.143
−28.109
1.00
12.99


ATOM
3077
O
LEU
A
402
−11.695
24.166
−28.707
1.00
13.19


ATOM
3078
N
ALA
A
403
−11.320
26.070
−27.578
1.00
13.02


ATOM
3079
CA
ALA
A
403
−9.884
25.870
−27.454
1.00
12.06


ATOM
3080
CB
ALA
A
403
−9.194
27.226
−27.220
1.00
11.77


ATOM
3081
C
ALA
A
403
−9.591
24.907
−26.300
1.00
12.81


ATOM
3082
O
ALA
A
403
−10.508
24.315
−25.714
1.00
12.36


ATOM
3083
N
GLU
A
404
−8.308
24.772
−25.959
1.00
11.39


ATOM
3084
CA
GLU
A
404
−7.918
23.922
−24.855
1.00
11.47


ATOM
3085
CB
GLU
A
404
−6.412
23.689
−24.931
1.00
11.02


ATOM
3086
CG
GLU
A
404
−5.865
22.723
−23.873
1.00
11.30


ATOM
3087
CD
GLU
A
404
−4.363
22.669
−23.954
1.00
12.25


ATOM
3088
OE1
GLU
A
404
−3.729
23.692
−23.622
1.00
12.06


ATOM
3089
OE2
GLU
A
404
−3.818
21.624
−24.390
1.00
12.60


ATOM
3090
C
GLU
A
404
−8.246
24.635
−23.538
1.00
11.77


ATOM
3091
O
GLU
A
404
−8.755
24.006
−22.590
1.00
11.08


ATOM
3092
N
GLN
A
405
−7.890
25.924
−23.453
1.00
12.07


ATOM
3093
CA
GLN
A
405
−7.952
26.655
−22.196
1.00
13.33


ATOM
3094
CB
GLN
A
405
−6.539
26.986
−21.678
1.00
13.24


ATOM
3095
CG
GLN
A
405
−5.625
25.821
−21.553
1.00
15.96


ATOM
3096
CD
GLN
A
405
−4.229
26.213
−21.051
1.00
14.97


ATOM
3097
OE1
GLN
A
405
−4.068
27.130
−20.236
1.00
15.09


ATOM
3098
NE2
GLN
A
405
−3.236
25.475
−21.496
1.00
15.87


ATOM
3099
C
GLN
A
405
−8.687
27.985
−22.356
1.00
13.51


ATOM
3100
O
GLN
A
405
−8.870
28.475
−23.489
1.00
13.74


ATOM
3101
N
PHE
A
406
−9.041
28.587
−21.226
1.00
12.60


ATOM
3102
CA
PHE
A
406
−9.516
29.990
−21.207
1.00
13.82


ATOM
3103
CB
PHE
A
406
−11.058
30.104
−21.232
1.00
13.56


ATOM
3104
CG
PHE
A
406
−11.800
29.385
−20.123
1.00
15.20


ATOM
3105
CD1
PHE
A
406
−12.155
28.026
−20.242
1.00
16.33


ATOM
3106
CE1
PHE
A
406
−12.879
27.370
−19.239
1.00
16.80


ATOM
3107
CZ
PHE
A
406
−13.340
28.094
−18.114
1.00
16.74


ATOM
3108
CE2
PHE
A
406
−13.020
29.453
−17.993
1.00
15.37


ATOM
3109
CD2
PHE
A
406
−12.260
30.101
−19.000
1.00
17.56


ATOM
3110
C
PHE
A
406
−8.836
30.737
−20.078
1.00
13.80


ATOM
3111
O
PHE
A
406
−8.587
30.151
−19.018
1.00
14.02


ATOM
3112
N
ASP
A
407
−8.481
31.997
−20.321
1.00
14.98


ATOM
3113
CA
ASP
A
407
−7.547
32.726
−19.438
1.00
15.04


ATOM
3114
CB
ASP
A
407
−7.237
34.115
−20.032
1.00
15.99


ATOM
3115
CG
ASP
A
407
−6.159
34.829
−19.293
1.00
18.17


ATOM
3116
OD1
ASP
A
407
−6.474
35.508
−18.293
1.00
20.41


ATOM
3117
OD2
ASP
A
407
−4.993
34.683
−19.685
1.00
20.75


ATOM
3118
C
ASP
A
407
−8.100
32.829
−18.005
1.00
14.54


ATOM
3119
O
ASP
A
407
−9.257
33.185
−17.800
1.00
15.12


ATOM
3120
N
ARG
A
408
−7.248
32.548
−17.018
1.00
14.63


ATOM
3121
CA
ARG
A
408
−7.644
32.530
−15.609
1.00
15.33


ATOM
3122
CB
ARG
A
408
−6.453
32.095
−14.753
1.00
15.32


ATOM
3123
CG
ARG
A
408
−5.236
33.062
−14.828
1.00
14.48


ATOM
3124
CD
ARG
A
408
−4.009
32.479
−14.122
1.00
16.22


ATOM
3125
NE
ARG
A
408
−4.237
32.248
−12.695
1.00
15.55


ATOM
3126
CZ
ARG
A
408
−3.613
31.323
−11.961
1.00
18.66


ATOM
3127
NH1
ARG
A
408
−3.878
31.231
−10.658
1.00
17.51


ATOM
3128
NH2
ARG
A
408
−2.717
30.499
−12.511
1.00
17.12


ATOM
3129
C
ARG
A
408
−8.167
33.886
−15.108
1.00
16.66


ATOM
3130
O
ARG
A
408
−8.898
33.943
−14.110
1.00
16.82


ATOM
3131
N
ASN
A
409
−7.781
34.964
−15.790
1.00
18.00


ATOM
3132
CA
ASN
A
409
−8.252
36.316
−15.421
1.00
20.06


ATOM
3133
CB
ASN
A
409
−7.069
37.275
−15.355
1.00
20.32


ATOM
3134
CG
ASN
A
409
−6.119
36.937
−14.224
1.00
21.65


ATOM
3135
OD1
ASN
A
409
−6.549
36.678
−13.111
1.00
24.42


ATOM
3136
ND2
ASN
A
409
−4.830
36.914
−14.516
1.00
23.96


ATOM
3137
C
ASN
A
409
−9.320
36.903
−16.336
1.00
21.43


ATOM
3138
O
ASN
A
409
−10.272
37.524
−15.857
1.00
22.03


ATOM
3139
N
SER
A
410
−9.152
36.724
−17.646
1.00
22.25


ATOM
3140
CA
SER
A
410
−10.007
37.410
−18.624
1.00
22.76


ATOM
3141
CB
SER
A
410
−9.146
38.159
−19.636
1.00
23.45


ATOM
3142
OG
SER
A
410
−8.470
37.260
−20.495
1.00
23.38


ATOM
3143
C
SER
A
410
−10.971
36.472
−19.343
1.00
22.82


ATOM
3144
O
SER
A
410
−11.898
36.925
−20.010
1.00
23.59


ATOM
3145
N
GLY
A
411
−10.758
35.161
−19.238
1.00
21.64


ATOM
3146
CA
GLY
A
411
−11.668
34.221
−19.877
1.00
20.39


ATOM
3147
C
GLY
A
411
−11.476
34.087
−21.379
1.00
19.99


ATOM
3148
O
GLY
A
411
−12.223
33.368
−22.029
1.00
20.85


ATOM
3149
N
THR
A
412
−10.478
34.750
−21.941
1.00
19.04


ATOM
3150
CA
THR
A
412
−10.268
34.658
−23.383
1.00
20.25


ATOM
3151
CB
THR
A
412
−9.447
35.853
−23.922
1.00
21.81


ATOM
3152
OG1
THR
A
412
−8.187
35.900
−23.257
1.00
26.24


ATOM
3153
CG2
THR
A
412
−10.160
37.163
−23.631
1.00
23.82


ATOM
3154
C
THR
A
412
−9.615
33.294
−23.732
1.00
19.17


ATOM
3155
O
THR
A
412
−8.786
32.796
−22.970
1.00
17.70


ATOM
3156
N
PRO
A
413
−9.996
32.688
−24.874
1.00
18.84


ATOM
3157
CA
PRO
A
413
−9.466
31.348
−25.234
1.00
18.32


ATOM
3158
CB
PRO
A
413
−10.220
31.002
−26.525
1.00
18.75


ATOM
3159
CG
PRO
A
413
−11.513
31.928
−26.451
1.00
19.42


ATOM
3160
CD
PRO
A
413
−10.943
33.195
−25.891
1.00
19.40


ATOM
3161
C
PRO
A
413
−7.959
31.399
−25.464
1.00
18.87


ATOM
3162
O
PRO
A
413
−7.436
32.406
−25.955
1.00
18.20


ATOM
3163
N
LEU
A
414
−7.253
30.353
−25.051
1.00
18.44


ATOM
3164
CA
LEU
A
414
−5.822
30.275
−25.305
1.00
18.90


ATOM
3165
CB
LEU
A
414
−4.992
30.974
−24.208
1.00
21.66


ATOM
3166
CG
LEU
A
414
−5.019
30.574
−22.754
1.00
24.35


ATOM
3167
CD1
LEU
A
414
−4.134
31.484
−21.892
1.00
27.42


ATOM
3168
CD2
LEU
A
414
−6.406
30.669
−22.224
1.00
30.77


ATOM
3169
C
LEU
A
414
−5.362
28.854
−25.518
1.00
17.24


ATOM
3170
O
LEU
A
414
−6.138
27.913
−25.406
1.00
15.97


ATOM
3171
N
SER
A
415
−4.091
28.733
−25.865
1.00
15.34


ATOM
3172
CA
SER
A
415
−3.473
27.481
−26.257
1.00
15.28


ATOM
3173
CB
SER
A
415
−3.434
26.468
−25.101
1.00
14.94


ATOM
3174
OG
SER
A
415
−2.632
25.355
−25.445
1.00
14.00


ATOM
3175
C
SER
A
415
−4.141
26.932
−27.528
1.00
15.13


ATOM
3176
O
SER
A
415
−4.665
27.718
−28.334
1.00
14.92


ATOM
3177
N
ALA
A
416
−4.097
25.618
−27.714
1.00
14.27


ATOM
3178
CA
ALA
A
416
−4.540
24.977
−28.976
1.00
14.25


ATOM
3179
CB
ALA
A
416
−4.380
23.486
−28.889
1.00
13.68


ATOM
3180
C
ALA
A
416
−5.981
25.314
−29.315
1.00
14.33


ATOM
3181
O
ALA
A
416
−6.854
25.216
−28.459
1.00
14.09


ATOM
3182
N
LEU
A
417
−6.223
25.680
−30.567
1.00
13.34


ATOM
3183
CA
LEU
A
417
−7.584
25.985
−31.006
1.00
14.22


ATOM
3184
CB
LEU
A
417
−7.536
26.931
−32.194
1.00
16.32


ATOM
3185
CG
LEU
A
417
−6.841
28.283
−31.942
1.00
18.75


ATOM
3186
CD1
LEU
A
417
−7.005
29.127
−33.163
1.00
23.81


ATOM
3187
CD2
LEU
A
417
−7.419
28.991
−30.712
1.00
21.76


ATOM
3188
C
LEU
A
417
−8.279
24.687
−31.413
1.00
13.18


ATOM
3189
O
LEU
A
417
−7.610
23.712
−31.775
1.00
13.19


ATOM
3190
N
HIS
A
418
−9.609
24.658
−31.311
1.00
12.26


ATOM
3191
CA
HIS
A
418
−10.399
23.496
−31.764
1.00
11.77


ATOM
3192
CB
HIS
A
418
−10.487
23.454
−33.303
1.00
13.41


ATOM
3193
CG
HIS
A
418
−11.294
24.566
−33.898
1.00
14.68


ATOM
3194
ND1
HIS
A
418
−12.646
24.717
−33.660
1.00
16.00


ATOM
3195
CE1
HIS
A
418
−13.095
25.762
−34.341
1.00
17.83


ATOM
3196
NE2
HIS
A
418
−12.085
26.290
−35.015
1.00
17.38


ATOM
3197
CD2
HIS
A
418
−10.948
25.557
−34.763
1.00
17.65


ATOM
3198
C
HIS
A
418
−9.826
22.187
−31.206
1.00
12.24


ATOM
3199
O
HIS
A
418
−9.540
21.250
−31.947
1.00
12.10


ATOM
3200
N
LEU
A
419
−9.656
22.116
−29.880
1.00
10.99


ATOM
3201
CA
LEU
A
419
−9.152
20.881
−29.301
1.00
10.46


ATOM
3202
CB
LEU
A
419
−8.742
21.069
−27.826
1.00
10.91


ATOM
3203
CG
LEU
A
419
−7.983
19.883
−27.220
1.00
10.16


ATOM
3204
CD1
LEU
A
419
−6.524
19.944
−27.669
1.00
11.45


ATOM
3205
CD2
LEU
A
419
−8.080
19.960
−25.657
1.00
10.73


ATOM
3206
C
LEU
A
419
−10.215
19.812
−29.398
1.00
10.49


ATOM
3207
O
LEU
A
419
−11.312
19.973
−28.863
1.00
10.67


ATOM
3208
N
THR
A
420
−9.860
18.686
−30.021
1.00
10.22


ATOM
3209
CA
THR
A
420
−10.833
17.629
−30.296
1.00
10.90


ATOM
3210
CB
THR
A
420
−10.201
16.460
−31.096
1.00
11.55


ATOM
3211
OG1
THR
A
420
−9.357
16.999
−32.115
1.00
12.00


ATOM
3212
CG2
THR
A
420
−11.310
15.625
−31.786
1.00
12.52


ATOM
3213
C
THR
A
420
−11.426
17.135
−28.995
1.00
11.43


ATOM
3214
O
THR
A
420
−12.648
16.903
−28.891
1.00
11.83


ATOM
3215
N
TRP
A
421
−10.569
16.987
−27.980
1.00
9.79


ATOM
3216
CA
TRP
A
421
−11.052
16.511
−26.688
1.00
11.67


ATOM
3217
CB
TRP
A
421
−9.839
16.184
−25.803
1.00
12.06


ATOM
3218
CG
TRP
A
421
−10.075
15.476
−24.508
1.00
11.94


ATOM
3219
CD1
TRP
A
421
−11.274
15.202
−23.881
1.00
14.46


ATOM
3220
NE1
TRP
A
421
−11.044
14.590
−22.663
1.00
14.38


ATOM
3221
CE2
TRP
A
421
−9.691
14.497
−22.468
1.00
13.52


ATOM
3222
CD2
TRP
A
421
−9.053
15.039
−23.616
1.00
13.01


ATOM
3223
CE3
TRP
A
421
−7.652
15.045
−23.680
1.00
15.38


ATOM
3224
CZ3
TRP
A
421
−6.932
14.503
−22.605
1.00
16.10


ATOM
3225
CH2
TRP
A
421
−7.603
13.973
−21.475
1.00
14.87


ATOM
3226
CZ2
TRP
A
421
−8.973
13.945
−21.398
1.00
14.27


ATOM
3227
C
TRP
A
421
−12.035
17.514
−26.032
1.00
10.89


ATOM
3228
O
TRP
A
421
−12.966
17.092
−25.357
1.00
10.96


ATOM
3229
N
SER
A
422
−11.844
18.822
−26.211
1.00
10.49


ATOM
3230
CA
SER
A
422
−12.833
19.794
−25.696
1.00
11.13


ATOM
3231
CB
SER
A
422
−12.459
21.243
−26.049
1.00
10.97


ATOM
3232
OG
SER
A
422
−11.302
21.682
−25.338
1.00
13.73


ATOM
3233
C
SER
A
422
−14.229
19.496
−26.257
1.00
11.40


ATOM
3234
O
SER
A
422
−15.204
19.468
−25.530
1.00
12.11


ATOM
3235
N
TYR
A
423
−14.320
19.281
−27.563
1.00
11.29


ATOM
3236
CA
TYR
A
423
−15.617
18.990
−28.170
1.00
11.29


ATOM
3237
CB
TYR
A
423
−15.502
19.020
−29.717
1.00
11.85


ATOM
3238
CG
TYR
A
423
−15.132
20.389
−30.274
1.00
12.18


ATOM
3239
CD1
TYR
A
423
−16.002
21.485
−30.145
1.00
10.35


ATOM
3240
CE1
TYR
A
423
−15.668
22.741
−30.643
1.00
11.83


ATOM
3241
CZ
TYR
A
423
−14.468
22.912
−31.316
1.00
12.29


ATOM
3242
OH
TYR
A
423
−14.157
24.145
−31.783
1.00
13.08


ATOM
3243
CE2
TYR
A
423
−13.588
21.845
−31.496
1.00
13.57


ATOM
3244
CD2
TYR
A
423
−13.942
20.572
−30.991
1.00
12.74


ATOM
3245
C
TYR
A
423
−16.217
17.673
−27.658
1.00
12.12


ATOM
3246
O
TYR
A
423
−17.430
17.622
−27.323
1.00
12.13


ATOM
3247
N
ALA
A
424
−15.385
16.623
−27.539
1.00
10.99


ATOM
3248
CA
ALA
A
424
−15.853
15.337
−26.986
1.00
11.26


ATOM
3249
CB
ALA
A
424
−14.717
14.294
−26.952
1.00
11.73


ATOM
3250
C
ALA
A
424
−16.411
15.535
−25.588
1.00
10.93


ATOM
3251
O
ALA
A
424
−17.465
14.974
−25.246
1.00
11.68


ATOM
3252
N
SER
A
425
−15.696
16.308
−24.770
1.00
9.74


ATOM
3253
CA
SER
A
425
−16.077
16.519
−23.379
1.00
10.92


ATOM
3254
CB
SER
A
425
−14.948
17.228
−22.602
1.00
11.59


ATOM
3255
OG
SER
A
425
−14.817
18.604
−22.957
1.00
13.86


ATOM
3256
C
SER
A
425
−17.402
17.291
−23.219
1.00
11.65


ATOM
3257
O
SER
A
425
−18.132
17.043
−22.276
1.00
11.86


ATOM
3258
N
PHE
A
426
−17.683
18.220
−24.133
1.00
12.13


ATOM
3259
CA
PHE
A
426
−18.983
18.891
−24.154
1.00
12.95


ATOM
3260
CB
PHE
A
426
−19.018
20.053
−25.173
1.00
13.14


ATOM
3261
CG
PHE
A
426
−20.410
20.575
−25.391
1.00
15.93


ATOM
3262
CD1
PHE
A
426
−20.951
21.522
−24.516
1.00
17.76


ATOM
3263
CE1
PHE
A
426
−22.279
21.980
−24.688
1.00
17.49


ATOM
3264
CZ
PHE
A
426
−23.064
21.455
−25.716
1.00
17.22


ATOM
3265
CE2
PHE
A
426
−22.561
20.496
−26.558
1.00
17.04


ATOM
3266
CD2
PHE
A
426
−21.225
20.045
−26.396
1.00
17.29


ATOM
3267
C
PHE
A
426
−20.079
17.878
−24.502
1.00
13.31


ATOM
3268
O
PHE
A
426
−21.120
17.827
−23.850
1.00
13.38


ATOM
3269
N
LEU
A
427
−19.834
17.077
−25.539
1.00
13.23


ATOM
3270
CA
LEU
A
427
−20.811
16.090
−25.996
1.00
14.41


ATOM
3271
CB
LEU
A
427
−20.339
15.410
−27.291
1.00
14.32


ATOM
3272
CG
LEU
A
427
−20.363
16.336
−28.506
1.00
15.96


ATOM
3273
CD1
LEU
A
427
−19.661
15.639
−29.689
1.00
18.66


ATOM
3274
CD2
LEU
A
427
−21.773
16.800
−28.876
1.00
16.20


ATOM
3275
C
LEU
A
427
−21.137
15.045
−24.959
1.00
14.86


ATOM
3276
O
LEU
A
427
−22.307
14.667
−24.833
1.00
16.01


ATOM
3277
N
THR
A
428
−20.130
14.551
−24.235
1.00
13.81


ATOM
3278
CA
THR
A
428
−20.397
13.544
−23.196
1.00
13.67


ATOM
3279
CB
THR
A
428
−19.134
12.732
−22.745
1.00
12.87


ATOM
3280
OG1
THR
A
428
−18.127
13.597
−22.185
1.00
12.33


ATOM
3281
CG2
THR
A
428
−18.533
11.902
−23.923
1.00
13.77


ATOM
3282
C
THR
A
428
−21.146
14.133
−21.980
1.00
13.66


ATOM
3283
O
THR
A
428
−22.102
13.517
−21.478
1.00
15.09


ATOM
3284
N
ALA
A
429
−20.738
15.312
−21.524
1.00
13.72


ATOM
3285
CA
ALA
A
429
−21.367
15.942
−20.355
1.00
14.65


ATOM
3286
CB
ALA
A
429
−20.704
17.295
−20.036
1.00
14.11


ATOM
3287
C
ALA
A
429
−22.852
16.158
−20.643
1.00
15.47


ATOM
3288
O
ALA
A
429
−23.704
15.908
−19.783
1.00
15.97


ATOM
3289
N
THR
A
430
−23.151
16.622
−21.854
1.00
15.48


ATOM
3290
CA
THR
A
430
−24.554
16.940
−22.208
1.00
15.64


ATOM
3291
CB
THR
A
430
−24.675
17.968
−23.353
1.00
16.50


ATOM
3292
OG1
THR
A
430
−23.980
17.494
−24.514
1.00
16.24


ATOM
3293
CG2
THR
A
430
−24.101
19.317
−22.916
1.00
16.43


ATOM
3294
C
THR
A
430
−25.401
15.690
−22.463
1.00
15.41


ATOM
3295
O
THR
A
430
−26.611
15.674
−22.158
1.00
15.67


ATOM
3296
N
ALA
A
431
−24.772
14.632
−22.968
1.00
15.22


ATOM
3297
CA
ALA
A
431
−25.437
13.311
−23.049
1.00
16.26


ATOM
3298
CB
ALA
A
431
−24.560
12.300
−23.762
1.00
16.39


ATOM
3299
C
ALA
A
431
−25.842
12.797
−21.673
1.00
16.53


ATOM
3300
O
ALA
A
431
−26.985
12.319
−21.485
1.00
16.61


ATOM
3301
N
ARG
A
432
−24.937
12.895
−20.689
1.00
15.66


ATOM
3302
CA
ARG
A
432
−25.256
12.410
−19.342
1.00
15.64


ATOM
3303
CB
ARG
A
432
−24.022
12.413
−18.432
1.00
15.83


ATOM
3304
CG
ARG
A
432
−22.862
11.574
−18.994
1.00
14.85


ATOM
3305
CD
ARG
A
432
−23.174
10.051
−19.154
1.00
17.10


ATOM
3306
NE
ARG
A
432
−21.958
9.472
−19.708
1.00
18.67


ATOM
3307
CZ
ARG
A
432
−21.766
9.225
−21.003
1.00
20.81


ATOM
3308
NH1
ARG
A
432
−22.769
9.372
−21.868
1.00
17.45


ATOM
3309
NH2
ARG
A
432
−20.576
8.781
−21.427
1.00
19.90


ATOM
3310
C
ARG
A
432
−26.375
13.235
−18.719
1.00
16.42


ATOM
3311
O
ARG
A
432
−27.256
12.685
−18.030
1.00
17.47


ATOM
3312
N
ARG
A
433
−26.371
14.535
−18.996
1.00
16.54


ATOM
3313
CA
ARG
A
433
−27.425
15.418
−18.493
1.00
17.60


ATOM
3314
CB
ARG
A
433
−27.204
16.852
−18.960
1.00
16.74


ATOM
3315
CG
ARG
A
433
−28.287
17.833
−18.461
1.00
18.39


ATOM
3316
CD
ARG
A
433
−27.931
19.239
−18.866
1.00
20.79


ATOM
3317
NE
ARG
A
433
−28.739
20.260
−18.166
1.00
23.05


ATOM
3318
CZ
ARG
A
433
−29.859
20.799
−18.654
1.00
26.97


ATOM
3319
NH1
ARG
A
433
−30.333
20.404
−19.837
1.00
24.94


ATOM
3320
NH2
ARG
A
433
−30.506
21.738
−17.954
1.00
25.73


ATOM
3321
C
ARG
A
433
−28.793
14.943
−18.956
1.00
17.90


ATOM
3322
O
ARG
A
433
−29.761
14.984
−18.184
1.00
18.50


ATOM
3323
N
ALA
A
434
−28.861
14.502
−20.210
1.00
18.06


ATOM
3324
CA
ALA
A
434
−30.103
13.993
−20.806
1.00
19.10


ATOM
3325
CB
ALA
A
434
−30.067
14.202
−22.318
1.00
19.95


ATOM
3326
C
ALA
A
434
−30.399
12.532
−20.475
1.00
20.48


ATOM
3327
O
ALA
A
434
−31.371
11.975
−20.980
1.00
21.63


ATOM
3328
N
GLY
A
435
−29.594
11.904
−19.620
1.00
19.94


ATOM
3329
CA
GLY
A
435
−29.865
10.531
−19.182
1.00
21.09


ATOM
3330
C
GLY
A
435
−29.415
9.475
−20.188
1.00
21.57


ATOM
3331
O
GLY
A
435
−29.847
8.304
−20.130
1.00
21.64


ATOM
3332
N
ILE
A
436
−28.529
9.873
−21.100
1.00
20.44


ATOM
3333
CA
ILE
A
436
−27.951
8.937
−22.060
1.00
20.81


ATOM
3334
CB
ILE
A
436
−27.753
9.601
−23.447
1.00
20.75


ATOM
3335
CG1
ILE
A
436
−29.132
9.977
−24.027
1.00
23.03


ATOM
3336
CD1
ILE
A
436
−29.103
11.031
−25.128
1.00
26.34


ATOM
3337
CG2
ILE
A
436
−27.031
8.643
−24.395
1.00
21.35


ATOM
3338
C
ILE
A
436
−26.634
8.412
−21.485
1.00
20.99


ATOM
3339
O
ILE
A
436
−25.666
9.171
−21.339
1.00
20.46


ATOM
3340
N
VAL
A
437
−26.616
7.120
−21.162
1.00
20.70


ATOM
3341
CA
VAL
A
437
−25.465
6.479
−20.517
1.00
21.39


ATOM
3342
CB
VAL
A
437
−25.848
5.826
−19.160
1.00
21.74


ATOM
3343
CG1
VAL
A
437
−26.340
6.911
−18.205
1.00
22.12


ATOM
3344
CG2
VAL
A
437
−26.909
4.703
−19.334
1.00
21.83


ATOM
3345
C
VAL
A
437
−24.802
5.459
−21.444
1.00
21.79


ATOM
3346
O
VAL
A
437
−25.459
4.901
−22.312
1.00
22.18


ATOM
3347
N
PRO
A
438
−23.497
5.208
−21.255
1.00
22.28


ATOM
3348
CA
PRO
A
438
−22.837
4.291
−22.181
1.00
22.83


ATOM
3349
CB
PRO
A
438
−21.365
4.642
−22.009
1.00
22.45


ATOM
3350
CG
PRO
A
438
−21.248
5.054
−20.578
1.00
23.88


ATOM
3351
CD
PRO
A
438
−22.575
5.707
−20.214
1.00
22.50


ATOM
3352
C
PRO
A
438
−23.093
2.840
−21.753
1.00
22.80


ATOM
3353
O
PRO
A
438
−23.580
2.604
−20.626
1.00
23.06


ATOM
3354
N
PRO
A
439
−22.796
1.878
−22.639
1.00
22.65


ATOM
3355
CA
PRO
A
439
−22.911
0.452
−22.283
1.00
22.08


ATOM
3356
CB
PRO
A
439
−22.300
−0.269
−23.499
1.00
21.30


ATOM
3357
CG
PRO
A
439
−22.526
0.664
−24.618
1.00
22.81


ATOM
3358
CD
PRO
A
439
−22.391
2.062
−24.050
1.00
22.29


ATOM
3359
C
PRO
A
439
−22.122
0.129
−21.037
1.00
21.88


ATOM
3360
O
PRO
A
439
−21.075
0.750
−20.776
1.00
20.95


ATOM
3361
N
SER
A
440
−22.628
−0.818
−20.253
1.00
22.34


ATOM
3362
CA
SER
A
440
−21.932
−1.273
−19.060
1.00
23.42


ATOM
3363
CB
SER
A
440
−22.818
−2.195
−18.224
1.00
24.01


ATOM
3364
OG
SER
A
440
−23.805
−1.412
−17.566
1.00
26.78


ATOM
3365
C
SER
A
440
−20.654
−1.992
−19.430
1.00
23.78


ATOM
3366
O
SER
A
440
−20.540
−2.554
−20.522
1.00
23.97


ATOM
3367
N
TRP
A
441
−19.681
−1.929
−18.536
1.00
24.15


ATOM
3368
CA
TRP
A
441
−18.431
−2.646
−18.718
1.00
24.63


ATOM
3369
CB
TRP
A
441
−17.255
−1.679
−18.819
1.00
22.62


ATOM
3370
CG
TRP
A
441
−16.963
−0.837
−17.583
1.00
19.24


ATOM
3371
CD1
TRP
A
441
−17.409
0.432
−17.339
1.00
16.95


ATOM
3372
NE1
TRP
A
441
−16.909
0.878
−16.138
1.00
17.89


ATOM
3373
CE2
TRP
A
441
−16.111
−0.098
−15.595
1.00
17.88


ATOM
3374
CD2
TRP
A
441
−16.130
−1.194
−16.476
1.00
18.22


ATOM
3375
CE3
TRP
A
441
−15.377
−2.338
−16.149
1.00
19.64


ATOM
3376
CZ3
TRP
A
441
−14.658
−2.355
−14.961
1.00
18.61


ATOM
3377
CH2
TRP
A
441
−14.670
−1.246
−14.094
1.00
20.62


ATOM
3378
CZ2
TRP
A
441
−15.400
−0.114
−14.391
1.00
19.39


ATOM
3379
C
TRP
A
441
−18.179
−3.661
−17.625
1.00
26.71


ATOM
3380
O
TRP
A
441
−17.410
−4.592
−17.813
1.00
25.60


ATOM
3381
N
ALA
A
442
−18.798
−3.471
−16.468
1.00
29.78


ATOM
3382
CA
ALA
A
442
−18.442
−4.292
−15.330
1.00
33.94


ATOM
3383
CB
ALA
A
442
−18.347
−3.447
−14.082
1.00
33.18


ATOM
3384
C
ALA
A
442
−19.447
−5.412
−15.136
1.00
37.28


ATOM
3385
O
ALA
A
442
−20.383
−5.561
−15.915
1.00
38.51


ATOM
3386
N
ASN
A
443
−19.201
−6.222
−14.116
1.00
41.45


ATOM
3387
CA
ASN
A
443
−20.226
−7.030
−13.467
1.00
45.10


ATOM
3388
CB
ASN
A
443
−20.135
−8.490
−13.914
1.00
45.75


ATOM
3389
CG
ASN
A
443
−18.815
−9.129
−13.531
1.00
48.14


ATOM
3390
OD1
ASN
A
443
−18.620
−9.524
−12.380
1.00
50.51


ATOM
3391
ND2
ASN
A
443
−17.888
−9.212
−14.492
1.00
50.01


ATOM
3392
C
ASN
A
443
−19.946
−6.892
−11.972
1.00
46.93


ATOM
3393
O
ASN
A
443
−18.878
−6.396
−11.580
1.00
47.13


ATOM
3394
N
SER
A
444
−20.873
−7.343
−11.135
1.00
49.21


ATOM
3395
CA
SER
A
444
−20.719
−7.201
−9.684
1.00
51.08


ATOM
3396
CB
SER
A
444
−21.713
−8.096
−8.936
1.00
51.21


ATOM
3397
OG
SER
A
444
−21.738
−7.735
−7.563
1.00
52.90


ATOM
3398
C
SER
A
444
−19.291
−7.452
−9.165
1.00
51.89


ATOM
3399
O
SER
A
444
−18.743
−6.610
−8.433
1.00
52.46


ATOM
3400
N
SER
A
445
−18.700
−8.588
−9.558
1.00
52.45


ATOM
3401
CA
SER
A
445
−17.400
−9.030
−9.029
1.00
52.98


ATOM
3402
CB
SER
A
445
−17.138
−10.497
−9.385
1.00
53.12


ATOM
3403
OG
SER
A
445
−16.901
−10.650
−10.775
1.00
54.45


ATOM
3404
C
SER
A
445
−16.202
−8.159
−9.449
1.00
52.99


ATOM
3405
O
SER
A
445
−15.184
−8.112
−8.738
1.00
53.28


ATOM
3406
N
ALA
A
446
−16.328
−7.472
−10.588
1.00
52.67


ATOM
3407
CA
ALA
A
446
−15.301
−6.536
−11.063
1.00
52.20


ATOM
3408
CB
ALA
A
446
−15.695
−5.960
−12.425
1.00
52.45


ATOM
3409
C
ALA
A
446
−14.996
−5.406
−10.059
1.00
51.92


ATOM
3410
O
ALA
A
446
−14.144
−4.553
−10.322
1.00
51.77


ATOM
3411
N
SER
A
447
−15.696
−5.413
−8.919
1.00
51.23


ATOM
3412
CA
SER
A
447
−15.471
−4.453
−7.827
1.00
50.73


ATOM
3413
CB
SER
A
447
−16.769
−3.706
−7.495
1.00
50.70


ATOM
3414
OG
SER
A
447
−17.765
−4.605
−7.021
1.00
50.91


ATOM
3415
C
SER
A
447
−14.886
−5.072
−6.537
1.00
50.31


ATOM
3416
O
SER
A
447
−14.604
−4.345
−5.580
1.00
50.16


ATOM
3417
N
THR
A
448
−14.704
−6.394
−6.508
1.00
49.55


ATOM
3418
CA
THR
A
448
−14.165
−7.070
−5.314
1.00
49.05


ATOM
3419
CB
THR
A
448
−14.579
−8.561
−5.233
1.00
49.15


ATOM
3420
OG1
THR
A
448
−14.076
−9.255
−6.378
1.00
49.97


ATOM
3421
CG2
THR
A
448
−16.096
−8.705
−5.180
1.00
49.11


ATOM
3422
C
THR
A
448
−12.641
−6.958
−5.215
1.00
48.34


ATOM
3423
O
THR
A
448
−11.911
−7.325
−6.135
1.00
47.84


ATOM
3424
N
ILE
A
449
−12.174
−6.444
−4.084
1.00
47.93


ATOM
3425
CA
ILE
A
449
−10.760
−6.150
−3.891
1.00
47.42


ATOM
3426
CB
ILE
A
449
−10.577
−4.798
−3.142
1.00
47.53


ATOM
3427
CG1
ILE
A
449
−11.346
−3.680
−3.863
1.00
46.60


ATOM
3428
CD1
ILE
A
449
−11.727
−2.523
−2.981
1.00
45.49


ATOM
3429
CG2
ILE
A
449
−9.097
−4.438
−2.999
1.00
46.84


ATOM
3430
C
ILE
A
449
−10.104
−7.299
−3.124
1.00
47.45


ATOM
3431
O
ILE
A
449
−10.606
−7.688
−2.067
1.00
47.66


ATOM
3432
N
PRO
A
450
−8.993
−7.857
−3.663
1.00
47.28


ATOM
3433
CA
PRO
A
450
−8.202
−8.926
−3.036
1.00
47.25


ATOM
3434
CB
PRO
A
450
−6.982
−9.040
−3.946
1.00
47.03


ATOM
3435
CG
PRO
A
450
−7.430
−8.535
−5.237
1.00
47.02


ATOM
3436
CD
PRO
A
450
−8.431
−7.470
−4.969
1.00
47.07


ATOM
3437
C
PRO
A
450
−7.731
−8.562
−1.639
1.00
47.48


ATOM
3438
O
PRO
A
450
−7.608
−7.377
−1.322
1.00
47.43


ATOM
3439
N
SER
A
451
−7.452
−9.587
−0.832
1.00
47.94


ATOM
3440
CA
SER
A
451
−7.020
−9.436
0.568
1.00
48.19


ATOM
3441
CB
SER
A
451
−7.017
−10.801
1.277
1.00
48.52


ATOM
3442
OG
SER
A
451
−8.297
−11.414
1.235
1.00
49.64


ATOM
3443
C
SER
A
451
−5.641
−8.799
0.701
1.00
47.83


ATOM
3444
O
SER
A
451
−5.415
−7.963
1.575
1.00
48.39


ATOM
3445
N
THR
A
452
−4.715
−9.212
−0.158
1.00
47.14


ATOM
3446
CA
THR
A
452
−3.379
−8.613
−0.211
1.00
46.42


ATOM
3447
CB
THR
A
452
−2.323
−9.540
0.434
1.00
46.66


ATOM
3448
OG1
THR
A
452
−2.518
−10.887
−0.032
1.00
48.38


ATOM
3449
CG2
THR
A
452
−2.446
−9.514
1.962
1.00
47.77


ATOM
3450
C
THR
A
452
−3.011
−8.323
−1.673
1.00
44.81


ATOM
3451
O
THR
A
452
−3.348
−9.107
−2.558
1.00
44.97


ATOM
3452
N
CYS
A
453
−2.363
−7.187
−1.931
1.00
43.37


ATOM
3453
CA
CYS
A
453
−1.971
−6.854
−3.306
1.00
41.40


ATOM
3454
CB
CYS
A
453
−1.918
−5.339
−3.574
1.00
41.01


ATOM
3455
SG
CYS
A
453
−3.187
−4.199
−2.908
1.00
40.58


ATOM
3456
C
CYS
A
453
−0.591
−7.408
−3.602
1.00
40.33


ATOM
3457
O
CYS
A
453
0.293
−7.370
−2.753
1.00
39.88


ATOM
3458
N
SER
A
454
−0.405
−7.911
−4.812
1.00
39.25


ATOM
3459
CA
SER
A
454
0.937
−8.142
−5.336
1.00
38.68


ATOM
3460
CB
SER
A
454
1.222
−9.638
−5.484
1.00
38.56


ATOM
3461
OG
SER
A
454
0.276
−10.251
−6.349
1.00
40.44


ATOM
3462
C
SER
A
454
1.047
−7.450
−6.690
1.00
37.78


ATOM
3463
O
SER
A
454
0.030
−7.187
−7.347
1.00
36.71


ATOM
3464
N
GLY
A
455
2.275
−7.175
−7.111
1.00
37.06


ATOM
3465
CA
GLY
A
455
2.514
−6.613
−8.431
1.00
36.79


ATOM
3466
C
GLY
A
455
2.493
−7.658
−9.539
1.00
36.18


ATOM
3467
O
GLY
A
455
3.410
−7.708
−10.367
1.00
36.73


ATOM
3468
N
ALA
A
456
1.445
−8.480
−9.562
1.00
35.26


ATOM
3469
CA
ALA
A
456
1.321
−9.584
−10.512
1.00
34.46


ATOM
3470
CB
ALA
A
456
0.125
−10.454
−10.158
1.00
34.50


ATOM
3471
C
ALA
A
456
1.195
−9.111
−11.963
1.00
34.16


ATOM
3472
O
ALA
A
456
0.283
−8.353
−12.301
1.00
33.56


ATOM
3473
N
SER
A
457
2.097
−9.588
−12.817
1.00
33.11


ATOM
3474
CA
SER
A
457
2.013
−9.302
−14.241
1.00
32.60


ATOM
3475
CB
SER
A
457
3.022
−8.219
−14.635
1.00
32.50


ATOM
3476
OG
SER
A
457
4.352
−8.691
−14.519
1.00
33.28


ATOM
3477
C
SER
A
457
2.228
−10.575
−15.044
1.00
32.31


ATOM
3478
O
SER
A
457
2.641
−11.605
−14.494
1.00
32.32


ATOM
3479
N
VAL
A
458
1.908
−10.511
−16.330
1.00
31.12


ATOM
3480
CA
VAL
A
458
2.063
−11.629
−17.246
1.00
31.09


ATOM
3481
CB
VAL
A
458
0.682
−12.199
−17.659
1.00
31.00


ATOM
3482
CG1
VAL
A
458
0.806
−13.173
−18.830
1.00
30.72


ATOM
3483
CG2
VAL
A
458
−0.014
−12.847
−16.459
1.00
31.08


ATOM
3484
C
VAL
A
458
2.817
−11.144
−18.480
1.00
30.88


ATOM
3485
O
VAL
A
458
2.401
−10.177
−19.126
1.00
29.70


ATOM
3486
N
VAL
A
459
3.924
−11.811
−18.805
1.00
30.81


ATOM
3487
CA
VAL
A
459
4.643
−11.525
−20.051
1.00
30.95


ATOM
3488
CB
VAL
A
459
6.046
−12.172
−20.071
1.00
31.34


ATOM
3489
CG1
VAL
A
459
6.664
−12.102
−21.492
1.00
30.45


ATOM
3490
CG2
VAL
A
459
6.947
−11.522
−19.030
1.00
32.03


ATOM
3491
C
VAL
A
459
3.805
−12.032
−21.227
1.00
31.37


ATOM
3492
O
VAL
A
459
3.443
−13.214
−21.288
1.00
31.84


ATOM
3493
N
GLY
A
460
3.480
−11.137
−22.154
1.00
30.71


ATOM
3494
CA
GLY
A
460
2.596
−11.495
−23.258
1.00
30.77


ATOM
3495
C
GLY
A
460
3.349
−11.799
−24.536
1.00
30.66


ATOM
3496
O
GLY
A
460
4.585
−11.773
−24.582
1.00
30.90


ATOM
3497
N
SER
A
461
2.606
−12.094
−25.584
1.00
30.44


ATOM
3498
CA
SER
A
461
3.219
−12.227
−26.877
1.00
31.06


ATOM
3499
CB
SER
A
461
3.301
−13.695
−27.308
1.00
31.44


ATOM
3500
OG
SER
A
461
2.018
−14.278
−27.419
1.00
34.77


ATOM
3501
C
SER
A
461
2.463
−11.357
−27.864
1.00
30.21


ATOM
3502
O
SER
A
461
1.246
−11.156
−27.736
1.00
30.87


ATOM
3503
N
TYR
A
462
3.192
−10.822
−28.836
1.00
28.84


ATOM
3504
CA
TYR
A
462
2.651
−9.797
−29.712
1.00
28.13


ATOM
3505
CB
TYR
A
462
3.365
−8.471
−29.426
1.00
26.74


ATOM
3506
CG
TYR
A
462
3.264
−8.098
−27.976
1.00
25.28


ATOM
3507
CD1
TYR
A
462
2.184
−7.335
−27.508
1.00
23.75


ATOM
3508
CE1
TYR
A
462
2.066
−7.022
−26.162
1.00
23.41


ATOM
3509
CZ
TYR
A
462
3.030
−7.458
−25.268
1.00
23.32


ATOM
3510
OH
TYR
A
462
2.907
−7.155
−23.941
1.00
23.50


ATOM
3511
CE2
TYR
A
462
4.113
−8.226
−25.698
1.00
22.57


ATOM
3512
CD2
TYR
A
462
4.223
−8.537
−27.050
1.00
24.71


ATOM
3513
C
TYR
A
462
2.844
−10.191
−31.152
1.00
28.83


ATOM
3514
O
TYR
A
462
3.898
−10.697
−31.529
1.00
29.33


ATOM
3515
N
SER
A
463
1.828
−9.973
−31.961
1.00
29.66


ATOM
3516
CA
SER
A
463
1.970
−10.202
−33.388
1.00
30.77


ATOM
3517
CB
SER
A
463
1.424
−11.574
−33.784
1.00
30.99


ATOM
3518
OG
SER
A
463
0.168
−11.815
−33.192
1.00
33.22


ATOM
3519
C
SER
A
463
1.311
−9.082
−34.170
1.00
31.28


ATOM
3520
O
SER
A
463
0.329
−8.481
−33.723
1.00
30.40


ATOM
3521
N
ARG
A
464
1.886
−8.789
−35.330
1.00
31.94


ATOM
3522
CA
ARG
A
464
1.377
−7.775
−36.225
1.00
32.97


ATOM
3523
CB
ARG
A
464
2.362
−7.620
−37.385
1.00
33.68


ATOM
3524
CG
ARG
A
464
2.353
−6.274
−38.061
1.00
36.94


ATOM
3525
CD
ARG
A
464
3.502
−6.206
−39.088
1.00
42.45


ATOM
3526
NE
ARG
A
464
4.794
−6.065
−38.415
1.00
45.53


ATOM
3527
CZ
ARG
A
464
5.416
−4.903
−38.227
1.00
47.50


ATOM
3528
NH1
ARG
A
464
4.882
−3.775
−38.688
1.00
49.50


ATOM
3529
NH2
ARG
A
464
6.580
−4.863
−37.592
1.00
48.59


ATOM
3530
C
ARG
A
464
−0.017
−8.171
−36.741
1.00
33.03


ATOM
3531
O
ARG
A
464
−0.166
−9.228
−37.358
1.00
33.00


ATOM
3532
N
PRO
A
465
−1.053
−7.333
−36.479
1.00
32.72


ATOM
3533
CA
PRO
A
465
−2.344
−7.593
−37.131
1.00
32.69


ATOM
3534
CB
PRO
A
465
−3.274
−6.504
−36.558
1.00
32.50


ATOM
3535
CG
PRO
A
465
−2.581
−5.981
−35.345
1.00
32.47


ATOM
3536
CD
PRO
A
465
−1.102
−6.134
−35.618
1.00
32.83


ATOM
3537
C
PRO
A
465
−2.189
−7.421
−38.642
1.00
33.30


ATOM
3538
O
PRO
A
465
−1.332
−6.661
−39.097
1.00
33.20


ATOM
3539
N
THR
A
466
−2.990
−8.136
−39.412
1.00
34.26


ATOM
3540
CA
THR
A
466
−2.810
−8.131
−40.855
1.00
35.62


ATOM
3541
CB
THR
A
466
−2.264
−9.486
−41.370
1.00
35.33


ATOM
3542
OG1
THR
A
466
−3.225
−10.512
−41.136
1.00
36.81


ATOM
3543
CG2
THR
A
466
−0.965
−9.848
−40.656
1.00
35.91


ATOM
3544
C
THR
A
466
−4.076
−7.711
−41.600
1.00
36.15


ATOM
3545
O
THR
A
466
−3.983
−7.077
−42.648
1.00
36.93


ATOM
3546
N
ALA
A
467
−5.242
−8.051
−41.048
1.00
36.82


ATOM
3547
CA
ALA
A
467
−6.540
−7.648
−41.609
1.00
37.30


ATOM
3548
CB
ALA
A
467
−7.663
−8.403
−40.930
1.00
37.04


ATOM
3549
C
ALA
A
467
−6.767
−6.136
−41.509
1.00
38.13


ATOM
3550
O
ALA
A
467
−6.715
−5.556
−40.417
1.00
37.96


ATOM
3551
N
THR
A
468
−7.011
−5.502
−42.653
1.00
38.66


ATOM
3552
CA
THR
A
468
−7.146
−4.050
−42.702
1.00
39.18


ATOM
3553
CB
THR
A
468
−5.970
−3.406
−43.428
1.00
39.43


ATOM
3554
OG1
THR
A
468
−5.955
−3.879
−44.778
1.00
40.06


ATOM
3555
CG2
THR
A
468
−4.637
−3.717
−42.734
1.00
39.67


ATOM
3556
C
THR
A
468
−8.405
−3.591
−43.427
1.00
39.33


ATOM
3557
O
THR
A
468
−8.468
−2.451
−43.890
1.00
39.79


ATOM
3558
N
SER
A
469
−9.403
−4.457
−43.529
1.00
39.12


ATOM
3559
CA
SER
A
469
−10.651
−4.065
−44.176
1.00
39.41


ATOM
3560
CB
SER
A
469
−10.624
−4.369
−45.684
1.00
39.77


ATOM
3561
OG
SER
A
469
−10.476
−5.763
−45.916
1.00
41.02


ATOM
3562
C
SER
A
469
−11.850
−4.732
−43.537
1.00
38.54


ATOM
3563
O
SER
A
469
−11.771
−5.856
−43.046
1.00
38.85


ATOM
3564
N
PHE
A
470
−12.962
−4.014
−43.558
1.00
38.00


ATOM
3565
CA
PHE
A
470
−14.216
−4.507
−43.031
1.00
37.29


ATOM
3566
CB
PHE
A
470
−14.880
−3.406
−42.220
1.00
36.87


ATOM
3567
CG
PHE
A
470
−14.277
−3.212
−40.865
1.00
35.43


ATOM
3568
CD1
PHE
A
470
−13.146
−2.428
−40.696
1.00
35.15


ATOM
3569
CE1
PHE
A
470
−12.589
−2.252
−39.437
1.00
33.73


ATOM
3570
CZ
PHE
A
470
−13.159
−2.852
−38.332
1.00
35.04


ATOM
3571
CE2
PHE
A
470
−14.292
−3.638
−38.479
1.00
36.26


ATOM
3572
CD2
PHE
A
470
−14.844
−3.816
−39.751
1.00
35.71


ATOM
3573
C
PHE
A
470
−15.128
−4.923
−44.181
1.00
37.34


ATOM
3574
O
PHE
A
470
−15.059
−4.337
−45.258
1.00
37.21


ATOM
3575
N
PRO
A
471
−15.987
−5.931
−43.959
1.00
37.46


ATOM
3576
CA
PRO
A
471
−16.983
−6.243
−44.985
1.00
38.04


ATOM
3577
CB
PRO
A
471
−17.790
−7.383
−44.361
1.00
37.60


ATOM
3578
CG
PRO
A
471
−16.877
−7.986
−43.337
1.00
38.09


ATOM
3579
CD
PRO
A
471
−16.093
−6.828
−42.795
1.00
37.31


ATOM
3580
C
PRO
A
471
−17.879
−5.033
−45.231
1.00
38.96


ATOM
3581
O
PRO
A
471
−18.108
−4.245
−44.306
1.00
38.78


ATOM
3582
N
PRO
A
472
−18.378
−4.869
−46.471
1.00
39.78


ATOM
3583
CA
PRO
A
472
−19.238
−3.723
−46.771
1.00
39.87


ATOM
3584
CB
PRO
A
472
−19.378
−3.780
−48.293
1.00
40.09


ATOM
3585
CG
PRO
A
472
−19.171
−5.225
−48.635
1.00
40.28


ATOM
3586
CD
PRO
A
472
−18.171
−5.740
−47.649
1.00
40.02


ATOM
3587
C
PRO
A
472
−20.604
−3.864
−46.114
1.00
39.75


ATOM
3588
O
PRO
A
472
−21.017
−4.980
−45.798
1.00
40.10


ATOM
3589
N
SER
A
473
−21.268
−2.734
−45.881
1.00
39.71


ATOM
3590
CA
SER
A
473
−22.675
−2.696
−45.467
1.00
39.61


ATOM
3591
CB
SER
A
473
−23.571
−3.070
−46.651
1.00
40.16


ATOM
3592
OG
SER
A
473
−23.468
−2.074
−47.658
1.00
42.55


ATOM
3593
C
SER
A
473
−23.043
−3.509
−44.221
1.00
38.94


ATOM
3594
O
SER
A
473
−24.041
−4.258
−44.210
1.00
39.07


ATOM
3595
N
GLN
A
474
−22.257
−3.340
−43.159
1.00
37.75


ATOM
3596
CA
GLN
A
474
−22.558
−3.993
−41.888
1.00
36.60


ATOM
3597
CB
GLN
A
474
−21.291
−4.205
−41.057
1.00
36.41


ATOM
3598
CG
GLN
A
474
−20.331
−5.169
−41.732
1.00
36.24


ATOM
3599
CD
GLN
A
474
−19.295
−5.757
−40.795
1.00
36.45


ATOM
3600
OE1
GLN
A
474
−18.478
−5.040
−40.211
1.00
35.30


ATOM
3601
NE2
GLN
A
474
−19.304
−7.077
−40.671
1.00
36.85


ATOM
3602
C
GLN
A
474
−23.620
−3.191
−41.149
1.00
36.40


ATOM
3603
O
GLN
A
474
−23.329
−2.444
−40.208
1.00
35.99


ATOM
3604
N
THR
A
475
−24.859
−3.350
−41.616
1.00
35.59


ATOM
3605
CA
THR
A
475
−26.012
−2.595
−41.148
1.00
35.20


ATOM
3606
CB
THR
A
475
−27.051
−2.478
−42.287
1.00
35.73


ATOM
3607
OG1
THR
A
475
−27.120
−3.737
−42.959
1.00
36.85


ATOM
3608
CG2
THR
A
475
−26.642
−1.418
−43.310
1.00
34.51


ATOM
3609
C
THR
A
475
−26.635
−3.256
−39.910
1.00
35.16


ATOM
3610
O
THR
A
475
−26.363
−4.420
−39.622
1.00
34.47


ATOM
3611
N
PRO
A
476
−27.453
−2.510
−39.148
1.00
35.36


ATOM
3612
CA
PRO
A
476
−27.990
−3.111
−37.923
1.00
36.40


ATOM
3613
CB
PRO
A
476
−28.567
−1.910
−37.167
1.00
36.04


ATOM
3614
CG
PRO
A
476
−28.890
−0.912
−38.230
1.00
35.98


ATOM
3615
CD
PRO
A
476
−27.907
−1.119
−39.339
1.00
35.70


ATOM
3616
C
PRO
A
476
−29.085
−4.171
−38.158
1.00
37.45


ATOM
3617
O
PRO
A
476
−29.654
−4.244
−39.254
1.00
36.95


ATOM
3618
N
LYS
A
477
−29.342
−4.984
−37.133
1.00
39.01


ATOM
3619
CA
LYS
A
477
−30.472
−5.911
−37.111
1.00
41.20


ATOM
3620
CB
LYS
A
477
−30.541
−6.641
−35.774
1.00
41.46


ATOM
3621
CG
LYS
A
477
−29.665
−7.850
−35.604
1.00
41.87


ATOM
3622
CD
LYS
A
477
−29.939
−8.517
−34.237
1.00
42.36


ATOM
3623
CE
LYS
A
477
−29.996
−7.497
−33.076
1.00
44.55


ATOM
3624
NZ
LYS
A
477
−29.705
−8.110
−31.718
1.00
44.56


ATOM
3625
C
LYS
A
477
−31.766
−5.118
−37.230
1.00
42.27


ATOM
3626
O
LYS
A
477
−31.818
−3.960
−36.798
1.00
42.18


ATOM
3627
N
PRO
A
478
−32.831
−5.743
−37.780
1.00
43.46


ATOM
3628
CA
PRO
A
478
−34.150
−5.106
−37.669
1.00
44.02


ATOM
3629
CB
PRO
A
478
−35.106
−6.144
−38.267
1.00
43.89


ATOM
3630
CG
PRO
A
478
−34.255
−7.033
−39.105
1.00
44.08


ATOM
3631
CD
PRO
A
478
−32.885
−7.034
−38.493
1.00
43.41


ATOM
3632
C
PRO
A
478
−34.480
−4.892
−36.194
1.00
44.52


ATOM
3633
O
PRO
A
478
−34.197
−5.769
−35.364
1.00
44.73


ATOM
3634
N
GLY
A
479
−35.043
−3.728
−35.874
1.00
45.20


ATOM
3635
CA
GLY
A
479
−35.421
−3.395
−34.494
1.00
45.90


ATOM
3636
C
GLY
A
479
−34.386
−2.601
−33.711
1.00
46.37


ATOM
3637
O
GLY
A
479
−34.576
−2.331
−32.520
1.00
46.98


ATOM
3638
N
VAL
A
480
−33.282
−2.244
−34.367
1.00
46.28


ATOM
3639
CA
VAL
A
480
−32.261
−1.383
−33.760
1.00
46.15


ATOM
3640
CB
VAL
A
480
−30.820
−1.863
−34.121
1.00
46.20


ATOM
3641
CG1
VAL
A
480
−29.755
−0.899
−33.584
1.00
45.85


ATOM
3642
CG2
VAL
A
480
−30.569
−3.281
−33.603
1.00
46.25


ATOM
3643
C
VAL
A
480
−32.498
0.046
−34.260
1.00
45.93


ATOM
3644
O
VAL
A
480
−32.673
0.240
−35.465
1.00
46.15


ATOM
3645
N
PRO
A
481
−32.534
1.049
−33.344
1.00
45.70


ATOM
3646
CA
PRO
A
481
−32.648
2.443
−33.804
1.00
45.36


ATOM
3647
CB
PRO
A
481
−32.388
3.266
−32.542
1.00
45.39


ATOM
3648
CG
PRO
A
481
−32.778
2.375
−31.427
1.00
45.52


ATOM
3649
CD
PRO
A
481
−32.481
0.962
−31.873
1.00
45.83


ATOM
3650
C
PRO
A
481
−31.609
2.762
−34.877
1.00
45.08


ATOM
3651
O
PRO
A
481
−30.405
2.555
−34.681
1.00
44.45


ATOM
3652
N
SER
A
482
−32.100
3.241
−36.011
1.00
44.83


ATOM
3653
CA
SER
A
482
−31.281
3.485
−37.180
1.00
44.52


ATOM
3654
CB
SER
A
482
−31.502
2.375
−38.211
1.00
44.74


ATOM
3655
OG
SER
A
482
−30.769
2.622
−39.399
1.00
45.89


ATOM
3656
C
SER
A
482
−31.661
4.836
−37.765
1.00
43.84


ATOM
3657
O
SER
A
482
−32.836
5.219
−37.741
1.00
44.07


ATOM
3658
N
GLY
A
483
−30.667
5.550
−38.282
1.00
42.68


ATOM
3659
CA
GLY
A
483
−30.872
6.872
−38.853
1.00
41.64


ATOM
3660
C
GLY
A
483
−30.085
7.095
−40.130
1.00
41.05


ATOM
3661
O
GLY
A
483
−29.430
6.179
−40.647
1.00
41.09


ATOM
3662
N
THR
A
484
−30.155
8.317
−40.647
1.00
40.22


ATOM
3663
CA
THR
A
484
−29.461
8.677
−41.888
1.00
39.91


ATOM
3664
CB
THR
A
484
−30.148
9.876
−42.619
1.00
40.21


ATOM
3665
OG1
THR
A
484
−30.115
11.040
−41.780
1.00
41.48


ATOM
3666
CG2
THR
A
484
−31.604
9.541
−43.000
1.00
40.86


ATOM
3667
C
THR
A
484
−27.995
9.033
−41.603
1.00
38.43


ATOM
3668
O
THR
A
484
−27.669
9.421
−40.483
1.00
38.52


ATOM
3669
N
PRO
A
485
−27.109
8.893
−42.612
1.00
37.36


ATOM
3670
CA
PRO
A
485
−25.695
9.226
−42.413
1.00
36.29


ATOM
3671
CB
PRO
A
485
−25.077
8.974
−43.795
1.00
36.75


ATOM
3672
CG
PRO
A
485
−25.997
7.985
−44.442
1.00
37.04


ATOM
3673
CD
PRO
A
485
−27.359
8.393
−43.976
1.00
37.30


ATOM
3674
C
PRO
A
485
−25.460
10.684
−41.988
1.00
35.20


ATOM
3675
O
PRO
A
485
−26.201
11.599
−42.396
1.00
34.39


ATOM
3676
N
TYR
A
486
−24.428
10.887
−41.174
1.00
33.60


ATOM
3677
CA
TYR
A
486
−24.025
12.233
−40.782
1.00
32.74


ATOM
3678
CB
TYR
A
486
−22.821
12.180
−39.826
1.00
32.31


ATOM
3679
CG
TYR
A
486
−22.348
13.564
−39.452
1.00
32.79


ATOM
3680
CD1
TYR
A
486
−21.243
14.146
−40.083
1.00
31.74


ATOM
3681
CE1
TYR
A
486
−20.827
15.430
−39.742
1.00
30.85


ATOM
3682
CZ
TYR
A
486
−21.527
16.141
−38.778
1.00
31.99


ATOM
3683
OH
TYR
A
486
−21.143
17.423
−38.427
1.00
32.20


ATOM
3684
CE2
TYR
A
486
−22.629
15.588
−38.160
1.00
31.88


ATOM
3685
CD2
TYR
A
486
−23.036
14.311
−38.500
1.00
32.49


ATOM
3686
C
TYR
A
486
−23.652
13.082
−41.999
1.00
31.99


ATOM
3687
O
TYR
A
486
−22.949
12.602
−42.900
1.00
31.62


ATOM
3688
N
THR
A
487
−24.106
14.336
−42.004
1.00
31.14


ATOM
3689
CA
THR
A
487
−23.676
15.336
−42.986
1.00
31.43


ATOM
3690
CB
THR
A
487
−24.879
15.785
−43.869
1.00
31.94


ATOM
3691
OG1
THR
A
487
−25.321
14.665
−44.644
1.00
35.19


ATOM
3692
CG2
THR
A
487
−24.489
16.904
−44.810
1.00
32.95


ATOM
3693
C
THR
A
487
−23.110
16.561
−42.261
1.00
29.79


ATOM
3694
O
THR
A
487
−23.761
17.078
−41.363
1.00
29.20


ATOM
3695
N
PRO
A
488
−21.901
17.027
−42.644
1.00
29.20


ATOM
3696
CA
PRO
A
488
−21.309
18.228
−42.005
1.00
28.77


ATOM
3697
CB
PRO
A
488
−19.988
18.435
−42.763
1.00
28.75


ATOM
3698
CG
PRO
A
488
−19.684
17.126
−43.408
1.00
29.52


ATOM
3699
CD
PRO
A
488
−21.010
16.448
−43.667
1.00
29.41


ATOM
3700
C
PRO
A
488
−22.175
19.463
−42.194
1.00
28.44


ATOM
3701
O
PRO
A
488
−23.003
19.499
−43.116
1.00
28.51


ATOM
3702
N
LEU
A
489
−21.971
20.469
−41.345
1.00
27.28


ATOM
3703
CA
LEU
A
489
−22.606
21.775
−41.522
1.00
26.52


ATOM
3704
CB
LEU
A
489
−22.269
22.708
−40.365
1.00
27.19


ATOM
3705
CG
LEU
A
489
−22.805
22.303
−38.987
1.00
27.53


ATOM
3706
CD1
LEU
A
489
−22.233
23.242
−37.929
1.00
26.81


ATOM
3707
CD2
LEU
A
489
−24.332
22.323
−38.970
1.00
28.98


ATOM
3708
C
LEU
A
489
−22.137
22.402
−42.833
1.00
26.65


ATOM
3709
O
LEU
A
489
−20.983
22.210
−43.245
1.00
25.40


ATOM
3710
N
PRO
A
490
−23.030
23.153
−43.503
1.00
26.84


ATOM
3711
CA
PRO
A
490
−22.636
23.745
−44.786
1.00
26.44


ATOM
3712
CB
PRO
A
490
−23.983
24.107
−45.432
1.00
27.00


ATOM
3713
CG
PRO
A
490
−24.900
24.341
−44.289
1.00
27.68


ATOM
3714
CD
PRO
A
490
−24.425
23.475
−43.137
1.00
26.91


ATOM
3715
C
PRO
A
490
−21.737
24.982
−44.668
1.00
26.22


ATOM
3716
O
PRO
A
490
−21.826
25.729
−43.698
1.00
25.74


ATOM
3717
N
CYS
A
491
−20.858
25.182
−45.650
1.00
26.06


ATOM
3718
CA
CYS
A
491
−20.079
26.412
−45.754
1.00
26.88


ATOM
3719
CB
CYS
A
491
−18.630
26.194
−45.302
1.00
27.00


ATOM
3720
SG
CYS
A
491
−18.450
25.196
−43.819
1.00
27.23


ATOM
3721
C
CYS
A
491
−20.032
26.822
−47.217
1.00
27.27


ATOM
3722
O
CYS
A
491
−20.369
26.026
−48.083
1.00
27.34


ATOM
3723
N
ALA
A
492
−19.577
28.045
−47.484
1.00
28.05


ATOM
3724
CA
ALA
A
492
−19.205
28.449
−48.845
1.00
29.40


ATOM
3725
CB
ALA
A
492
−18.837
29.928
−48.866
1.00
29.49


ATOM
3726
C
ALA
A
492
−18.023
27.599
−49.320
1.00
30.37


ATOM
3727
O
ALA
A
492
−17.310
26.998
−48.497
1.00
30.36


ATOM
3728
N
THR
A
493
−17.828
27.508
−50.633
1.00
31.27


ATOM
3729
CA
THR
A
493
−16.612
26.883
−51.163
1.00
32.43


ATOM
3730
CB
THR
A
493
−16.845
26.234
−52.533
1.00
33.22


ATOM
3731
OG1
THR
A
493
−17.944
25.324
−52.431
1.00
38.55


ATOM
3732
CG2
THR
A
493
−15.590
25.464
−52.996
1.00
33.71


ATOM
3733
C
THR
A
493
−15.596
28.006
−51.254
1.00
31.35


ATOM
3734
O
THR
A
493
−15.916
29.068
−51.795
1.00
31.58


ATOM
3735
N
PRO
A
494
−14.390
27.815
−50.682
1.00
30.44


ATOM
3736
CA
PRO
A
494
−13.464
28.947
−50.696
1.00
30.01


ATOM
3737
CB
PRO
A
494
−12.414
28.555
−49.645
1.00
30.26


ATOM
3738
CG
PRO
A
494
−12.416
27.077
−49.658
1.00
30.56


ATOM
3739
CD
PRO
A
494
−13.815
26.635
−49.997
1.00
30.85


ATOM
3740
C
PRO
A
494
−12.809
29.089
−52.060
1.00
28.81


ATOM
3741
O
PRO
A
494
−12.801
28.137
−52.834
1.00
28.82


ATOM
3742
N
THR
A
495
−12.260
30.258
−52.352
1.00
28.43


ATOM
3743
CA
THR
A
495
−11.551
30.419
−53.623
1.00
28.44


ATOM
3744
CB
THR
A
495
−11.885
31.748
−54.319
1.00
28.68


ATOM
3745
OG1
THR
A
495
−11.449
32.839
−53.500
1.00
30.39


ATOM
3746
CG2
THR
A
495
−13.383
31.858
−54.564
1.00
29.85


ATOM
3747
C
THR
A
495
−10.057
30.335
−53.404
1.00
27.94


ATOM
3748
O
THR
A
495
−9.289
30.159
−54.352
1.00
27.63


ATOM
3749
N
SER
A
496
−9.671
30.463
−52.139
1.00
27.51


ATOM
3750
CA
SER
A
496
−8.279
30.492
−51.722
1.00
27.18


ATOM
3751
CB
SER
A
496
−7.928
31.916
−51.329
1.00
27.72


ATOM
3752
OG
SER
A
496
−6.531
32.076
−51.240
1.00
32.60


ATOM
3753
C
SER
A
496
−8.134
29.583
−50.501
1.00
25.85


ATOM
3754
O
SER
A
496
−9.024
29.548
−49.634
1.00
25.10


ATOM
3755
N
VAL
A
497
−7.039
28.824
−50.430
1.00
24.43


ATOM
3756
CA
VAL
A
497
−6.801
28.032
−49.209
1.00
22.17


ATOM
3757
CB
VAL
A
497
−7.281
26.511
−49.290
1.00
23.00


ATOM
3758
CG1
VAL
A
497
−6.224
25.445
−48.881
1.00
22.48


ATOM
3759
CG2
VAL
A
497
−8.049
26.161
−50.578
1.00
22.62


ATOM
3760
C
VAL
A
497
−5.388
28.251
−48.672
1.00
21.13


ATOM
3761
O
VAL
A
497
−4.419
28.359
−49.439
1.00
20.38


ATOM
3762
N
ALA
A
498
−5.302
28.395
−47.355
1.00
19.70


ATOM
3763
CA
ALA
A
498
−4.020
28.576
−46.702
1.00
18.92


ATOM
3764
CB
ALA
A
498
−4.226
29.126
−45.290
1.00
19.31


ATOM
3765
C
ALA
A
498
−3.396
27.185
−46.655
1.00
18.67


ATOM
3766
O
ALA
A
498
−3.966
26.266
−46.047
1.00
19.29


ATOM
3767
N
VAL
A
499
−2.252
27.021
−47.319
1.00
17.23


ATOM
3768
CA
VAL
A
499
−1.551
25.735
−47.361
1.00
16.09


ATOM
3769
CB
VAL
A
499
−1.165
25.347
−48.814
1.00
16.97


ATOM
3770
CG1
VAL
A
499
−0.403
23.984
−48.863
1.00
16.08


ATOM
3771
CG2
VAL
A
499
−2.413
25.291
−49.696
1.00
17.03


ATOM
3772
C
VAL
A
499
−0.306
25.841
−46.491
1.00
15.95


ATOM
3773
O
VAL
A
499
0.604
26.607
−46.791
1.00
16.15


ATOM
3774
N
THR
A
500
−0.279
25.085
−45.404
1.00
15.16


ATOM
3775
CA
THR
A
500
0.863
25.116
−44.505
1.00
14.85


ATOM
3776
CB
THR
A
500
0.415
24.916
−43.035
1.00
14.76


ATOM
3777
OG1
THR
A
500
−0.403
26.022
−42.635
1.00
16.00


ATOM
3778
CG2
THR
A
500
1.639
24.856
−42.136
1.00
15.39


ATOM
3779
C
THR
A
500
1.796
23.993
−44.932
1.00
14.99


ATOM
3780
O
THR
A
500
1.480
22.804
−44.792
1.00
14.81


ATOM
3781
N
PHE
A
501
2.941
24.370
−45.481
1.00
14.71


ATOM
3782
CA
PHE
A
501
3.981
23.411
−45.793
1.00
15.64


ATOM
3783
CB
PHE
A
501
4.943
23.964
−46.832
1.00
15.86


ATOM
3784
CG
PHE
A
501
4.289
24.172
−48.168
1.00
18.38


ATOM
3785
CD1
PHE
A
501
3.676
25.388
−48.469
1.00
19.85


ATOM
3786
CE1
PHE
A
501
3.052
25.581
−49.709
1.00
21.58


ATOM
3787
CZ
PHE
A
501
3.015
24.547
−50.642
1.00
19.80


ATOM
3788
CE2
PHE
A
501
3.607
23.324
−50.356
1.00
21.69


ATOM
3789
CD2
PHE
A
501
4.231
23.134
−49.095
1.00
21.96


ATOM
3790
C
PHE
A
501
4.711
23.009
−44.536
1.00
15.81


ATOM
3791
O
PHE
A
501
5.207
23.852
−43.804
1.00
16.78


ATOM
3792
N
HIS
A
502
4.789
21.698
−44.317
1.00
14.85


ATOM
3793
CA
HIS
A
502
5.239
21.175
−43.027
1.00
14.17


ATOM
3794
CB
HIS
A
502
3.987
20.565
−42.356
1.00
14.85


ATOM
3795
CG
HIS
A
502
4.221
19.875
−41.054
1.00
13.69


ATOM
3796
ND1
HIS
A
502
4.819
18.637
−40.966
1.00
12.55


ATOM
3797
CE1
HIS
A
502
4.816
18.241
−39.702
1.00
15.76


ATOM
3798
NE2
HIS
A
502
4.191
19.155
−38.980
1.00
14.42


ATOM
3799
CD2
HIS
A
502
3.797
20.183
−39.804
1.00
14.92


ATOM
3800
C
HIS
A
502
6.317
20.161
−43.412
1.00
14.16


ATOM
3801
O
HIS
A
502
6.013
19.043
−43.824
1.00
13.85


ATOM
3802
N
GLU
A
503
7.577
20.590
−43.352
1.00
13.63


ATOM
3803
CA
GLU
A
503
8.678
19.821
−43.968
1.00
14.46


ATOM
3804
CB
GLU
A
503
9.434
20.712
−44.996
1.00
14.22


ATOM
3805
CG
GLU
A
503
10.782
20.121
−45.524
1.00
16.31


ATOM
3806
CD
GLU
A
503
10.620
18.973
−46.539
1.00
21.32


ATOM
3807
OE1
GLU
A
503
11.523
18.819
−47.393
1.00
21.21


ATOM
3808
OE2
GLU
A
503
9.609
18.230
−46.510
1.00
20.10


ATOM
3809
C
GLU
A
503
9.657
19.322
−42.917
1.00
14.22


ATOM
3810
O
GLU
A
503
10.175
20.121
−42.131
1.00
15.31


ATOM
3811
N
LEU
A
504
9.960
18.027
−42.927
1.00
14.92


ATOM
3812
CA
LEU
A
504
11.026
17.518
−42.052
1.00
15.49


ATOM
3813
CB
LEU
A
504
10.658
16.147
−41.489
1.00
16.33


ATOM
3814
CG
LEU
A
504
9.479
16.178
−40.498
1.00
17.19


ATOM
3815
CD1
LEU
A
504
8.922
14.753
−40.320
1.00
19.08


ATOM
3816
CD2
LEU
A
504
9.953
16.723
−39.198
1.00
17.28


ATOM
3817
C
LEU
A
504
12.318
17.428
−42.846
1.00
16.59


ATOM
3818
O
LEU
A
504
12.403
16.656
−43.785
1.00
16.72


ATOM
3819
N
VAL
A
505
13.317
18.201
−42.444
1.00
17.20


ATOM
3820
CA
VAL
A
505
14.592
18.235
−43.154
1.00
19.16


ATOM
3821
CB
VAL
A
505
14.548
19.141
−44.418
1.00
18.88


ATOM
3822
CG1
VAL
A
505
14.028
20.539
−44.090
1.00
19.28


ATOM
3823
CG2
VAL
A
505
15.948
19.219
−45.095
1.00
21.65


ATOM
3824
C
VAL
A
505
15.674
18.705
−42.188
1.00
20.00


ATOM
3825
O
VAL
A
505
15.595
19.785
−41.595
1.00
19.92


ATOM
3826
N
SER
A
506
16.685
17.868
−42.011
1.00
21.73


ATOM
3827
CA
SER
A
506
17.761
18.216
−41.104
1.00
23.33


ATOM
3828
CB
SER
A
506
18.570
16.974
−40.771
1.00
23.74


ATOM
3829
OG
SER
A
506
19.583
17.320
−39.847
1.00
28.30


ATOM
3830
C
SER
A
506
18.646
19.284
−41.759
1.00
23.03


ATOM
3831
O
SER
A
506
19.070
19.139
−42.908
1.00
23.20


ATOM
3832
N
THR
A
507
18.888
20.371
−41.049
1.00
24.01


ATOM
3833
CA
THR
A
507
19.685
21.464
−41.600
1.00
25.15


ATOM
3834
CB
THR
A
507
18.845
22.725
−41.833
1.00
25.01


ATOM
3835
OG1
THR
A
507
18.104
23.015
−40.650
1.00
24.43


ATOM
3836
CG2
THR
A
507
17.891
22.536
−43.000
1.00
24.71


ATOM
3837
C
THR
A
507
20.795
21.812
−40.623
1.00
27.09


ATOM
3838
O
THR
A
507
20.729
21.448
−39.451
1.00
26.15


ATOM
3839
N
GLN
A
508
21.798
22.536
−41.113
1.00
29.34


ATOM
3840
CA
GLN
A
508
22.912
22.986
−40.272
1.00
32.54


ATOM
3841
CB
GLN
A
508
24.239
22.542
−40.897
1.00
32.54


ATOM
3842
CG
GLN
A
508
24.369
21.010
−40.972
1.00
34.32


ATOM
3843
CD
GLN
A
508
25.400
20.515
−41.991
1.00
36.23


ATOM
3844
OE1
GLN
A
508
26.283
19.700
−41.660
1.00
41.79


ATOM
3845
NE2
GLN
A
508
25.279
20.977
−43.242
1.00
40.42


ATOM
3846
C
GLN
A
508
22.827
24.502
−40.100
1.00
33.06


ATOM
3847
O
GLN
A
508
22.136
25.178
−40.873
1.00
32.49


ATOM
3848
N
PHE
A
509
23.494
25.037
−39.075
1.00
33.80


ATOM
3849
CA
PHE
A
509
23.432
26.476
−38.782
1.00
35.03


ATOM
3850
CB
PHE
A
509
24.413
26.837
−37.651
1.00
36.75


ATOM
3851
CG
PHE
A
509
24.481
28.315
−37.340
1.00
39.07


ATOM
3852
CD1
PHE
A
509
23.592
28.893
−36.428
1.00
41.58


ATOM
3853
CE1
PHE
A
509
23.642
30.265
−36.140
1.00
42.61


ATOM
3854
CZ
PHE
A
509
24.603
31.073
−36.766
1.00
41.78


ATOM
3855
CE2
PHE
A
509
25.507
30.503
−37.678
1.00
42.48


ATOM
3856
CD2
PHE
A
509
25.441
29.127
−37.955
1.00
41.46


ATOM
3857
C
PHE
A
509
23.712
27.311
−40.040
1.00
34.58


ATOM
3858
O
PHE
A
509
24.614
26.990
−40.815
1.00
34.58


ATOM
3859
N
GLY
A
510
22.912
28.355
−40.256
1.00
33.85


ATOM
3860
CA
GLY
A
510
23.101
29.241
−41.407
1.00
33.22


ATOM
3861
C
GLY
A
510
22.352
28.826
−42.671
1.00
32.36


ATOM
3862
O
GLY
A
510
22.369
29.545
−43.679
1.00
32.97


ATOM
3863
N
GLN
A
511
21.705
27.663
−42.628
1.00
30.50


ATOM
3864
CA
GLN
A
511
20.885
27.217
−43.745
1.00
29.02


ATOM
3865
CB
GLN
A
511
21.026
25.712
−43.931
1.00
28.92


ATOM
3866
CG
GLN
A
511
22.436
25.276
−44.349
1.00
29.91


ATOM
3867
CD
GLN
A
511
22.571
23.776
−44.439
1.00
31.36


ATOM
3868
OE1
GLN
A
511
21.760
23.036
−43.879
1.00
31.69


ATOM
3869
NE2
GLN
A
511
23.590
23.309
−45.160
1.00
30.72


ATOM
3870
C
GLN
A
511
19.418
27.619
−43.543
1.00
27.82


ATOM
3871
O
GLN
A
511
18.928
27.695
−42.399
1.00
27.36


ATOM
3872
N
THR
A
512
18.727
27.895
−44.650
1.00
25.92


ATOM
3873
CA
THR
A
512
17.305
28.271
−44.613
1.00
24.51


ATOM
3874
CB
THR
A
512
17.126
29.763
−44.994
1.00
24.95


ATOM
3875
OG1
THR
A
512
17.769
30.580
−44.004
1.00
27.43


ATOM
3876
CG2
THR
A
512
15.653
30.151
−45.069
1.00
25.94


ATOM
3877
C
THR
A
512
16.536
27.384
−45.600
1.00
23.09


ATOM
3878
O
THR
A
512
16.994
27.152
−46.717
1.00
22.75


ATOM
3879
N
VAL
A
513
15.376
26.877
−45.200
1.00
20.62


ATOM
3880
CA
VAL
A
513
14.593
26.074
−46.136
1.00
19.05


ATOM
3881
CB
VAL
A
513
13.946
24.855
−45.428
1.00
19.18


ATOM
3882
CG1
VAL
A
513
13.041
24.064
−46.397
1.00
18.87


ATOM
3883
CG2
VAL
A
513
15.042
23.938
−44.895
1.00
20.56


ATOM
3884
C
VAL
A
513
13.536
26.979
−46.748
1.00
17.98


ATOM
3885
O
VAL
A
513
12.910
27.768
−46.029
1.00
16.65


ATOM
3886
N
LYS
A
514
13.346
26.857
−48.063
1.00
17.91


ATOM
3887
CA
LYS
A
514
12.279
27.583
−48.757
1.00
18.14


ATOM
3888
CB
LYS
A
514
12.845
28.712
−49.638
1.00
17.66


ATOM
3889
CG
LYS
A
514
13.867
29.576
−48.945
1.00
19.26


ATOM
3890
CD
LYS
A
514
14.197
30.839
−49.765
1.00
21.27


ATOM
3891
CE
LYS
A
514
15.224
31.675
−49.001
1.00
26.06


ATOM
3892
NZ
LYS
A
514
15.461
33.022
−49.626
1.00
28.70


ATOM
3893
C
LYS
A
514
11.494
26.621
−49.625
1.00
18.22


ATOM
3894
O
LYS
A
514
11.949
25.502
−49.912
1.00
18.39


ATOM
3895
N
VAL
A
515
10.304
27.037
−50.045
1.00
18.20


ATOM
3896
CA
VAL
A
515
9.546
26.212
−50.980
1.00
19.00


ATOM
3897
CB
VAL
A
515
8.198
25.731
−50.404
1.00
20.00


ATOM
3898
CG1
VAL
A
515
7.403
26.904
−49.879
1.00
21.01


ATOM
3899
CG2
VAL
A
515
7.417
24.903
−51.447
1.00
20.10


ATOM
3900
C
VAL
A
515
9.421
26.973
−52.302
1.00
18.89


ATOM
3901
O
VAL
A
515
9.079
28.159
−52.317
1.00
18.67


ATOM
3902
N
ALA
A
516
9.781
26.295
−53.390
1.00
19.87


ATOM
3903
CA
ALA
A
516
9.796
26.898
−54.732
1.00
20.38


ATOM
3904
CB
ALA
A
516
11.177
26.768
−55.356
1.00
20.58


ATOM
3905
C
ALA
A
516
8.789
26.110
−55.525
1.00
20.65


ATOM
3906
O
ALA
A
516
8.638
24.910
−55.303
1.00
20.40


ATOM
3907
N
GLY
A
517
8.075
26.765
−56.430
1.00
20.80


ATOM
3908
CA
GLY
A
517
7.092
26.039
−57.214
1.00
22.32


ATOM
3909
C
GLY
A
517
6.536
26.853
−58.352
1.00
22.86


ATOM
3910
O
GLY
A
517
6.902
28.024
−58.527
1.00
22.58


ATOM
3911
N
ASN
A
518
5.642
26.233
−59.116
1.00
24.66


ATOM
3912
CA
ASN
A
518
5.201
26.817
−60.390
1.00
26.74


ATOM
3913
CB
ASN
A
518
4.670
25.754
−61.354
1.00
26.97


ATOM
3914
CG
ASN
A
518
3.386
25.117
−60.872
1.00
31.69


ATOM
3915
OD1
ASN
A
518
3.004
25.226
−59.677
1.00
28.89


ATOM
3916
ND2
ASN
A
518
2.707
24.419
−61.786
1.00
31.65


ATOM
3917
C
ASN
A
518
4.199
27.937
−60.232
1.00
27.20


ATOM
3918
O
ASN
A
518
4.154
28.822
−61.079
1.00
28.68


ATOM
3919
N
ALA
A
519
3.399
27.907
−59.163
1.00
27.04


ATOM
3920
CA
ALA
A
519
2.424
28.978
−58.898
1.00
26.47


ATOM
3921
CB
ALA
A
519
1.473
28.598
−57.747
1.00
27.02


ATOM
3922
C
ALA
A
519
3.090
30.322
−58.629
1.00
26.35


ATOM
3923
O
ALA
A
519
4.226
30.394
−58.135
1.00
25.27


ATOM
3924
N
ALA
A
520
2.369
31.394
−58.954
1.00
26.25


ATOM
3925
CA
ALA
A
520
2.887
32.741
−58.784
1.00
26.77


ATOM
3926
CB
ALA
A
520
1.872
33.775
−59.298
1.00
27.50


ATOM
3927
C
ALA
A
520
3.250
33.004
−57.317
1.00
26.68


ATOM
3928
O
ALA
A
520
4.301
33.560
−57.030
1.00
26.01


ATOM
3929
N
ALA
A
521
2.395
32.548
−56.399
1.00
26.75


ATOM
3930
CA
ALA
A
521
2.628
32.712
−54.963
1.00
26.82


ATOM
3931
CB
ALA
A
521
1.395
32.251
−54.167
1.00
26.85


ATOM
3932
C
ALA
A
521
3.876
31.950
−54.504
1.00
26.51


ATOM
3933
O
ALA
A
521
4.485
32.305
−53.494
1.00
26.63


ATOM
3934
N
LEU
A
522
4.261
30.919
−55.259
1.00
26.79


ATOM
3935
CA
LEU
A
522
5.452
30.113
−54.932
1.00
26.50


ATOM
3936
CB
LEU
A
522
5.185
28.626
−55.155
1.00
26.64


ATOM
3937
CG
LEU
A
522
4.224
27.946
−54.169
1.00
26.58


ATOM
3938
CD1
LEU
A
522
4.049
26.489
−54.533
1.00
27.59


ATOM
3939
CD2
LEU
A
522
4.718
28.092
−52.730
1.00
28.08


ATOM
3940
C
LEU
A
522
6.696
30.559
−55.709
1.00
26.54


ATOM
3941
O
LEU
A
522
7.779
29.987
−55.547
1.00
25.56


ATOM
3942
N
GLY
A
523
6.518
31.575
−56.552
1.00
26.25


ATOM
3943
CA
GLY
A
523
7.637
32.267
−57.199
1.00
26.16


ATOM
3944
C
GLY
A
523
7.996
31.809
−58.607
1.00
26.84


ATOM
3945
O
GLY
A
523
9.029
32.227
−59.152
1.00
25.81


ATOM
3946
N
ASN
A
524
7.162
30.946
−59.193
1.00
27.13


ATOM
3947
CA
ASN
A
524
7.413
30.419
−60.539
1.00
27.74


ATOM
3948
CB
ASN
A
524
7.046
31.484
−61.591
1.00
28.43


ATOM
3949
CG
ASN
A
524
7.123
30.960
−63.015
1.00
30.79


ATOM
3950
OD1
ASN
A
524
6.856
29.780
−63.285
1.00
30.80


ATOM
3951
ND2
ASN
A
524
7.515
31.838
−63.936
1.00
33.61


ATOM
3952
C
ASN
A
524
8.845
29.857
−60.710
1.00
28.44


ATOM
3953
O
ASN
A
524
9.531
30.104
−61.720
1.00
27.82


ATOM
3954
N
TRP
A
525
9.280
29.111
−59.693
1.00
27.99


ATOM
3955
CA
TRP
A
525
10.573
28.398
−59.659
1.00
28.92


ATOM
3956
CB
TRP
A
525
10.787
27.507
−60.896
1.00
28.31


ATOM
3957
CG
TRP
A
525
9.803
26.394
−61.060
1.00
27.68


ATOM
3958
CD1
TRP
A
525
8.902
26.247
−62.078
1.00
27.96


ATOM
3959
NE1
TRP
A
525
8.166
25.106
−61.907
1.00
27.55


ATOM
3960
CE2
TRP
A
525
8.589
24.471
−60.762
1.00
30.58


ATOM
3961
CD2
TRP
A
525
9.609
25.277
−60.184
1.00
27.84


ATOM
3962
CE3
TRP
A
525
10.230
24.842
−59.001
1.00
26.50


ATOM
3963
CZ3
TRP
A
525
9.787
23.655
−58.411
1.00
27.55


ATOM
3964
CH2
TRP
A
525
8.752
22.889
−58.998
1.00
27.90


ATOM
3965
CZ2
TRP
A
525
8.144
23.279
−60.168
1.00
26.22


ATOM
3966
C
TRP
A
525
11.790
29.301
−59.452
1.00
29.66


ATOM
3967
O
TRP
A
525
12.921
28.804
−59.346
1.00
30.61


ATOM
3968
N
SER
A
526
11.570
30.613
−59.380
1.00
30.33


ATOM
3969
CA
SER
A
526
12.645
31.536
−59.004
1.00
31.13


ATOM
3970
CB
SER
A
526
12.213
32.993
−59.187
1.00
31.02


ATOM
3971
OG
SER
A
526
13.166
33.838
−58.562
1.00
33.69


ATOM
3972
C
SER
A
526
13.086
31.312
−57.560
1.00
31.29


ATOM
3973
O
SER
A
526
12.271
31.381
−56.627
1.00
31.21


ATOM
3974
N
THR
A
527
14.373
31.049
−57.367
1.00
31.34


ATOM
3975
CA
THR
A
527
14.880
30.794
−56.021
1.00
31.64


ATOM
3976
CB
THR
A
527
16.259
30.098
−56.024
1.00
31.79


ATOM
3977
OG1
THR
A
527
17.217
30.931
−56.682
1.00
31.43


ATOM
3978
CG2
THR
A
527
16.169
28.739
−56.724
1.00
32.27


ATOM
3979
C
THR
A
527
14.911
32.045
−55.152
1.00
31.99


ATOM
3980
O
THR
A
527
14.847
31.959
−53.922
1.00
32.36


ATOM
3981
N
SER
A
528
14.986
33.209
−55.787
1.00
31.79


ATOM
3982
CA
SER
A
528
14.928
34.463
−55.054
1.00
32.02


ATOM
3983
CB
SER
A
528
15.517
35.615
−55.885
1.00
32.70


ATOM
3984
OG
SER
A
528
14.712
35.882
−57.031
1.00
34.94


ATOM
3985
C
SER
A
528
13.497
34.784
−54.579
1.00
31.23


ATOM
3986
O
SER
A
528
13.330
35.435
−53.550
1.00
32.02


ATOM
3987
N
ALA
A
529
12.479
34.314
−55.306
1.00
29.33


ATOM
3988
CA
ALA
A
529
11.093
34.506
−54.893
1.00
27.60


ATOM
3989
CB
ALA
A
529
10.211
34.864
−56.086
1.00
27.34


ATOM
3990
C
ALA
A
529
10.482
33.328
−54.112
1.00
26.40


ATOM
3991
O
ALA
A
529
9.311
33.382
−53.754
1.00
26.54


ATOM
3992
N
ALA
A
530
11.268
32.286
−53.842
1.00
25.12


ATOM
3993
CA
ALA
A
530
10.777
31.114
−53.096
1.00
24.19


ATOM
3994
CB
ALA
A
530
11.855
30.063
−52.991
1.00
23.88


ATOM
3995
C
ALA
A
530
10.336
31.555
−51.706
1.00
23.55


ATOM
3996
O
ALA
A
530
10.848
32.540
−51.182
1.00
23.61


ATOM
3997
N
VAL
A
531
9.396
30.833
−51.110
1.00
22.18


ATOM
3998
CA
VAL
A
531
8.851
31.248
−49.821
1.00
22.62


ATOM
3999
CB
VAL
A
531
7.380
30.821
−49.677
1.00
22.81


ATOM
4000
CG1
VAL
A
531
6.815
31.335
−48.346
1.00
25.06


ATOM
4001
CG2
VAL
A
531
6.551
31.353
−50.886
1.00
25.38


ATOM
4002
C
VAL
A
531
9.659
30.646
−48.674
1.00
21.29


ATOM
4003
O
VAL
A
531
9.768
29.425
−48.564
1.00
20.72


ATOM
4004
N
ALA
A
532
10.215
31.493
−47.819
1.00
20.86


ATOM
4005
CA
ALA
A
532
11.008
30.999
−46.698
1.00
20.26


ATOM
4006
CB
ALA
A
532
11.850
32.128
−46.084
1.00
21.02


ATOM
4007
C
ALA
A
532
10.093
30.356
−45.646
1.00
20.51


ATOM
4008
O
ALA
A
532
9.019
30.884
−45.337
1.00
20.05


ATOM
4009
N
LEU
A
533
10.514
29.200
−45.129
1.00
19.00


ATOM
4010
CA
LEU
A
533
9.855
28.565
−43.999
1.00
18.65


ATOM
4011
CB
LEU
A
533
9.901
27.029
−44.148
1.00
18.14


ATOM
4012
CG
LEU
A
533
9.395
26.450
−45.483
1.00
19.25


ATOM
4013
CD1
LEU
A
533
9.385
24.923
−45.427
1.00
21.28


ATOM
4014
CD2
LEU
A
533
8.030
26.980
−45.894
1.00
18.58


ATOM
4015
C
LEU
A
533
10.541
29.014
−42.702
1.00
18.95


ATOM
4016
O
LEU
A
533
11.622
29.648
−42.744
1.00
18.94


ATOM
4017
N
ASP
A
534
9.905
28.715
−41.570
1.00
18.56


ATOM
4018
CA
ASP
A
534
10.381
29.096
−40.238
1.00
18.36


ATOM
4019
CB
ASP
A
534
9.220
29.634
−39.374
1.00
19.76


ATOM
4020
CG
ASP
A
534
8.757
30.992
−39.798
1.00
23.55


ATOM
4021
OD1
ASP
A
534
7.548
31.264
−39.659
1.00
26.14


ATOM
4022
OD2
ASP
A
534
9.600
31.774
−40.283
1.00
27.39


ATOM
4023
C
ASP
A
534
10.877
27.867
−39.504
1.00
17.68


ATOM
4024
O
ASP
A
534
10.310
26.780
−39.667
1.00
16.10


ATOM
4025
N
ALA
A
535
11.883
28.057
−38.654
1.00
17.05


ATOM
4026
CA
ALA
A
535
12.405
26.950
−37.835
1.00
17.76


ATOM
4027
CB
ALA
A
535
13.926
26.952
−37.832
1.00
17.96


ATOM
4028
C
ALA
A
535
11.872
27.027
−36.403
1.00
17.82


ATOM
4029
O
ALA
A
535
12.482
26.490
−35.474
1.00
18.36


ATOM
4030
N
VAL
A
536
10.745
27.706
−36.225
1.00
17.68


ATOM
4031
CA
VAL
A
536
10.138
27.861
−34.898
1.00
18.65


ATOM
4032
CB
VAL
A
536
8.824
28.719
−34.975
1.00
18.66


ATOM
4033
CG1
VAL
A
536
7.805
28.123
−35.971
1.00
19.98


ATOM
4034
CG2
VAL
A
536
8.208
28.962
−33.570
1.00
20.19


ATOM
4035
C
VAL
A
536
9.938
26.514
−34.155
1.00
18.51


ATOM
4036
O
VAL
A
536
10.124
26.437
−32.923
1.00
19.61


ATOM
4037
N
ASN
A
537
9.570
25.468
−34.883
1.00
18.59


ATOM
4038
CA
ASN
A
537
9.344
24.154
−34.261
1.00
19.07


ATOM
4039
CB
ASN
A
537
8.074
23.498
−34.816
1.00
19.28


ATOM
4040
CG
ASN
A
537
6.800
24.252
−34.448
1.00
21.02


ATOM
4041
OD1
ASN
A
537
6.742
24.940
−33.435
1.00
24.12


ATOM
4042
ND2
ASN
A
537
5.762
24.089
−35.265
1.00
20.99


ATOM
4043
C
ASN
A
537
10.518
23.182
−34.445
1.00
19.15


ATOM
4044
O
ASN
A
537
10.394
21.971
−34.196
1.00
18.60


ATOM
4045
N
TYR
A
538
11.653
23.699
−34.897
1.00
19.05


ATOM
4046
CA
TYR
A
538
12.767
22.830
−35.234
1.00
20.32


ATOM
4047
CB
TYR
A
538
13.816
23.618
−36.026
1.00
20.37


ATOM
4048
CG
TYR
A
538
14.916
22.747
−36.588
1.00
20.37


ATOM
4049
CD1
TYR
A
538
14.822
22.238
−37.886
1.00
20.39


ATOM
4050
CE1
TYR
A
538
15.853
21.436
−38.437
1.00
20.49


ATOM
4051
CZ
TYR
A
538
16.961
21.137
−37.670
1.00
20.65


ATOM
4052
OH
TYR
A
538
17.946
20.341
−38.218
1.00
21.53


ATOM
4053
CE2
TYR
A
538
17.066
21.602
−36.361
1.00
22.05


ATOM
4054
CD2
TYR
A
538
16.043
22.418
−35.825
1.00
21.95


ATOM
4055
C
TYR
A
538
13.436
22.209
−33.981
1.00
21.29


ATOM
4056
O
TYR
A
538
13.733
22.919
−33.036
1.00
21.89


ATOM
4057
N
ALA
A
539
13.695
20.902
−34.014
1.00
22.15


ATOM
4058
CA
ALA
A
539
14.646
20.258
−33.083
1.00
23.67


ATOM
4059
CB
ALA
A
539
13.909
19.536
−31.976
1.00
23.94


ATOM
4060
C
ALA
A
539
15.545
19.289
−33.849
1.00
24.35


ATOM
4061
O
ALA
A
539
15.117
18.698
−34.833
1.00
23.63


ATOM
4062
N
ASP
A
540
16.793
19.118
−33.405
1.00
25.69


ATOM
4063
CA
ASP
A
540
17.722
18.196
−34.099
1.00
27.42


ATOM
4064
CB
ASP
A
540
19.044
18.051
−33.339
1.00
28.62


ATOM
4065
CG
ASP
A
540
19.724
19.368
−33.140
1.00
33.80


ATOM
4066
OD1
ASP
A
540
19.875
20.115
−34.147
1.00
36.83


ATOM
4067
OD2
ASP
A
540
20.080
19.663
−31.970
1.00
40.32


ATOM
4068
C
ASP
A
540
17.150
16.818
−34.400
1.00
26.63


ATOM
4069
O
ASP
A
540
17.386
16.277
−35.485
1.00
27.28


ATOM
4070
N
ASN
A
541
16.403
16.247
−33.458
1.00
25.08


ATOM
4071
CA
ASN
A
541
15.827
14.922
−33.687
1.00
24.03


ATOM
4072
CB
ASN
A
541
15.988
14.032
−32.441
1.00
25.14


ATOM
4073
CG
ASN
A
541
15.337
14.623
−31.191
1.00
27.81


ATOM
4074
OD1
ASN
A
541
15.366
14.001
−30.118
1.00
31.57


ATOM
4075
ND2
ASN
A
541
14.771
15.824
−31.306
1.00
28.81


ATOM
4076
C
ASN
A
541
14.349
14.991
−34.118
1.00
22.46


ATOM
4077
O
ASN
A
541
13.660
13.979
−34.172
1.00
22.35


ATOM
4078
N
HIS
A
542
13.871
16.197
−34.403
1.00
19.85


ATOM
4079
CA
HIS
A
542
12.580
16.354
−35.083
1.00
18.22


ATOM
4080
CB
HIS
A
542
11.426
16.392
−34.062
1.00
16.89


ATOM
4081
CG
HIS
A
542
10.071
16.341
−34.699
1.00
17.32


ATOM
4082
ND1
HIS
A
542
9.211
17.417
−34.711
1.00
16.28


ATOM
4083
CE1
HIS
A
542
8.111
17.088
−35.371
1.00
14.64


ATOM
4084
NE2
HIS
A
542
8.217
15.834
−35.765
1.00
15.41


ATOM
4085
CD2
HIS
A
542
9.435
15.340
−35.358
1.00
15.14


ATOM
4086
C
HIS
A
542
12.662
17.650
−35.902
1.00
17.48


ATOM
4087
O
HIS
A
542
12.198
18.698
−35.446
1.00
17.79


ATOM
4088
N
PRO
A
543
13.324
17.584
−37.083
1.00
17.00


ATOM
4089
CA
PRO
A
543
13.797
18.752
−37.832
1.00
17.09


ATOM
4090
CB
PRO
A
543
14.948
18.164
−38.677
1.00
16.57


ATOM
4091
CG
PRO
A
543
14.472
16.759
−38.994
1.00
18.22


ATOM
4092
CD
PRO
A
543
13.676
16.321
−37.764
1.00
17.39


ATOM
4093
C
PRO
A
543
12.718
19.435
−38.691
1.00
16.46


ATOM
4094
O
PRO
A
543
12.811
19.497
−39.922
1.00
17.09


ATOM
4095
N
LEU
A
544
11.726
19.987
−38.009
1.00
15.36


ATOM
4096
CA
LEU
A
544
10.534
20.535
−38.636
1.00
15.10


ATOM
4097
CB
LEU
A
544
9.341
20.376
−37.672
1.00
15.29


ATOM
4098
CG
LEU
A
544
7.968
20.927
−38.134
1.00
16.53


ATOM
4099
CD1
LEU
A
544
7.524
20.364
−39.494
1.00
15.92


ATOM
4100
CD2
LEU
A
544
6.900
20.700
−37.062
1.00
14.59


ATOM
4101
C
LEU
A
544
10.694
22.018
−39.025
1.00
15.09


ATOM
4102
O
LEU
A
544
11.037
22.851
−38.197
1.00
15.34


ATOM
4103
N
TRP
A
545
10.456
22.298
−40.303
1.00
15.03


ATOM
4104
CA
TRP
A
545
10.327
23.637
−40.843
1.00
15.36


ATOM
4105
CB
TRP
A
545
11.288
23.790
−42.023
1.00
15.40


ATOM
4106
CG
TRP
A
545
12.758
23.921
−41.663
1.00
15.62


ATOM
4107
CD1
TRP
A
545
13.653
22.903
−41.384
1.00
16.85


ATOM
4108
NE1
TRP
A
545
14.906
23.437
−41.129
1.00
18.69


ATOM
4109
CE2
TRP
A
545
14.837
24.803
−41.246
1.00
17.78


ATOM
4110
CD2
TRP
A
545
13.498
25.140
−41.584
1.00
17.01


ATOM
4111
CE3
TRP
A
545
13.163
26.488
−41.777
1.00
17.17


ATOM
4112
CZ3
TRP
A
545
14.165
27.456
−41.637
1.00
18.29


ATOM
4113
CH2
TRP
A
545
15.483
27.085
−41.295
1.00
17.22


ATOM
4114
CZ2
TRP
A
545
15.835
25.767
−41.111
1.00
19.09


ATOM
4115
C
TRP
A
545
8.907
23.832
−41.359
1.00
15.14


ATOM
4116
O
TRP
A
545
8.327
22.933
−41.986
1.00
14.45


ATOM
4117
N
ILE
A
546
8.362
25.025
−41.149
1.00
15.19


ATOM
4118
CA
ILE
A
546
6.938
25.244
−41.428
1.00
16.51


ATOM
4119
CB
ILE
A
546
6.107
24.988
−40.130
1.00
17.39


ATOM
4120
CG1
ILE
A
546
4.615
24.852
−40.420
1.00
20.44


ATOM
4121
CD1
ILE
A
546
3.882
23.992
−39.392
1.00
23.59


ATOM
4122
CG2
ILE
A
546
6.391
26.064
−39.050
1.00
17.70


ATOM
4123
C
ILE
A
546
6.674
26.635
−42.006
1.00
16.75


ATOM
4124
O
ILE
A
546
7.352
27.593
−41.647
1.00
15.81


ATOM
4125
N
ALA
A
547
5.716
26.743
−42.925
1.00
16.88


ATOM
4126
CA
ALA
A
547
5.197
28.057
−43.279
1.00
17.94


ATOM
4127
CB
ALA
A
547
6.222
28.893
−43.931
1.00
21.49


ATOM
4128
C
ALA
A
547
4.009
27.919
−44.167
1.00
18.29


ATOM
4129
O
ALA
A
547
3.727
26.828
−44.655
1.00
18.52


ATOM
4130
N
THR
A
548
3.316
29.031
−44.362
1.00
17.99


ATOM
4131
CA
THR
A
548
1.970
29.017
−44.919
1.00
18.72


ATOM
4132
CB
THR
A
548
0.929
29.419
−43.855
1.00
18.65


ATOM
4133
OG1
THR
A
548
1.000
28.500
−42.751
1.00
19.46


ATOM
4134
CG2
THR
A
548
−0.491
29.379
−44.438
1.00
18.79


ATOM
4135
C
THR
A
548
1.865
29.960
−46.104
1.00
19.60


ATOM
4136
O
THR
A
548
2.347
31.090
−46.040
1.00
19.83


ATOM
4137
N
VAL
A
549
1.227
29.485
−47.164
1.00
20.41


ATOM
4138
CA
VAL
A
549
1.048
30.280
−48.389
1.00
22.36


ATOM
4139
CB
VAL
A
549
1.944
29.722
−49.537
1.00
22.77


ATOM
4140
CG1
VAL
A
549
1.717
30.491
−50.845
1.00
25.80


ATOM
4141
CG2
VAL
A
549
3.429
29.781
−49.148
1.00
24.78


ATOM
4142
C
VAL
A
549
−0.399
30.119
−48.800
1.00
22.21


ATOM
4143
O
VAL
A
549
−0.943
29.018
−48.719
1.00
21.56


ATOM
4144
N
ASN
A
550
−1.028
31.211
−49.240
1.00
22.80


ATOM
4145
CA
ASN
A
550
−2.356
31.107
−49.831
1.00
23.95


ATOM
4146
CB
ASN
A
550
−3.114
32.411
−49.649
1.00
24.39


ATOM
4147
CG
ASN
A
550
−3.367
32.706
−48.201
1.00
27.42


ATOM
4148
OD1
ASN
A
550
−3.811
31.838
−47.462
1.00
28.69


ATOM
4149
ND2
ASN
A
550
−3.041
33.911
−47.771
1.00
31.89


ATOM
4150
C
ASN
A
550
−2.278
30.733
−51.294
1.00
23.98


ATOM
4151
O
ASN
A
550
−1.598
31.400
−52.065
1.00
24.73


ATOM
4152
N
LEU
A
551
−2.973
29.667
−51.662
1.00
24.24


ATOM
4153
CA
LEU
A
551
−3.016
29.180
−53.020
1.00
24.78


ATOM
4154
CB
LEU
A
551
−2.348
27.797
−53.135
1.00
25.00


ATOM
4155
CG
LEU
A
551
−0.858
27.721
−52.787
1.00
25.19


ATOM
4156
CD1
LEU
A
551
−0.356
26.284
−52.803
1.00
27.45


ATOM
4157
CD2
LEU
A
551
−0.018
28.613
−53.718
1.00
26.73


ATOM
4158
C
LEU
A
551
−4.471
29.104
−53.488
1.00
25.46


ATOM
4159
O
LEU
A
551
−5.393
29.018
−52.675
1.00
24.27


ATOM
4160
N
GLU
A
552
−4.661
29.148
−54.804
1.00
26.15


ATOM
4161
CA
GLU
A
552
−6.004
29.165
−55.366
1.00
28.20


ATOM
4162
CB
GLU
A
552
−5.955
29.639
−56.823
1.00
28.30


ATOM
4163
CG
GLU
A
552
−7.326
29.739
−57.494
1.00
32.28


ATOM
4164
CD
GLU
A
552
−7.250
30.281
−58.926
1.00
33.44


ATOM
4165
OE1
GLU
A
552
−8.110
31.126
−59.274
1.00
41.80


ATOM
4166
OE2
GLU
A
552
−6.340
29.873
−59.695
1.00
39.14


ATOM
4167
C
GLU
A
552
−6.610
27.768
−55.253
1.00
26.97


ATOM
4168
O
GLU
A
552
−5.979
26.783
−55.622
1.00
26.49


ATOM
4169
N
ALA
A
553
−7.822
27.684
−54.723
1.00
27.21


ATOM
4170
CA
ALA
A
553
−8.502
26.399
−54.603
1.00
27.89


ATOM
4171
CB
ALA
A
553
−9.876
26.574
−53.953
1.00
28.37


ATOM
4172
C
ALA
A
553
−8.637
25.773
−55.979
1.00
28.57


ATOM
4173
O
ALA
A
553
−8.900
26.477
−56.952
1.00
28.83


ATOM
4174
N
GLY
A
554
−8.438
24.465
−56.064
1.00
28.44


ATOM
4175
CA
GLY
A
554
−8.556
23.753
−57.330
1.00
29.66


ATOM
4176
C
GLY
A
554
−7.274
23.693
−58.145
1.00
30.04


ATOM
4177
O
GLY
A
554
−7.122
22.814
−59.000
1.00
30.33


ATOM
4178
N
ASP
A
555
−6.347
24.606
−57.869
1.00
30.23


ATOM
4179
CA
ASP
A
555
−5.098
24.716
−58.630
1.00
30.61


ATOM
4180
CB
ASP
A
555
−4.313
25.939
−58.161
1.00
30.85


ATOM
4181
CG
ASP
A
555
−3.382
26.503
−59.236
1.00
34.49


ATOM
4182
OD1
ASP
A
555
−3.441
26.053
−60.407
1.00
37.19


ATOM
4183
OD2
ASP
A
555
−2.572
27.408
−58.901
1.00
36.59


ATOM
4184
C
ASP
A
555
−4.238
23.467
−58.486
1.00
30.14


ATOM
4185
O
ASP
A
555
−4.156
22.882
−57.419
1.00
30.07


ATOM
4186
N
VAL
A
556
−3.602
23.046
−59.572
1.00
29.55


ATOM
4187
CA
VAL
A
556
−2.628
21.963
−59.492
1.00
28.74


ATOM
4188
CB
VAL
A
556
−2.732
20.987
−60.680
1.00
29.35


ATOM
4189
CG1
VAL
A
556
−1.666
19.877
−60.569
1.00
28.99


ATOM
4190
CG2
VAL
A
556
−4.125
20.365
−60.720
1.00
30.44


ATOM
4191
C
VAL
A
556
−1.261
22.623
−59.448
1.00
28.04


ATOM
4192
O
VAL
A
556
−0.869
23.336
−60.384
1.00
27.25


ATOM
4193
N
VAL
A
557
−0.544
22.389
−58.352
1.00
26.81


ATOM
4194
CA
VAL
A
557
0.739
23.040
−58.100
1.00
26.14


ATOM
4195
CB
VAL
A
557
0.690
23.859
−56.759
1.00
26.71


ATOM
4196
CG1
VAL
A
557
2.073
24.140
−56.219
1.00
27.82


ATOM
4197
CG2
VAL
A
557
−0.088
25.175
−56.952
1.00
27.34


ATOM
4198
C
VAL
A
557
1.856
21.999
−58.092
1.00
25.64


ATOM
4199
O
VAL
A
557
1.646
20.845
−57.693
1.00
25.24


ATOM
4200
N
GLU
A
558
3.035
22.409
−58.553
1.00
24.16


ATOM
4201
CA
GLU
A
558
4.223
21.579
−58.516
1.00
24.38


ATOM
4202
CB
GLU
A
558
4.737
21.296
−59.933
1.00
24.86


ATOM
4203
CG
GLU
A
558
4.064
20.108
−60.606
1.00
26.34


ATOM
4204
CD
GLU
A
558
4.670
19.790
−61.962
1.00
27.68


ATOM
4205
OE1
GLU
A
558
5.917
19.684
−62.065
1.00
30.56


ATOM
4206
OE2
GLU
A
558
3.883
19.638
−62.915
1.00
32.45


ATOM
4207
C
GLU
A
558
5.262
22.337
−57.730
1.00
22.54


ATOM
4208
O
GLU
A
558
5.389
23.550
−57.883
1.00
22.93


ATOM
4209
N
TYR
A
559
5.992
21.640
−56.867
1.00
21.45


ATOM
4210
CA
TYR
A
559
6.927
22.346
−55.995
1.00
19.96


ATOM
4211
CB
TYR
A
559
6.188
22.972
−54.784
1.00
19.42


ATOM
4212
CG
TYR
A
559
5.624
21.952
−53.796
1.00
18.65


ATOM
4213
CD1
TYR
A
559
6.383
21.524
−52.703
1.00
18.64


ATOM
4214
CE1
TYR
A
559
5.887
20.595
−51.794
1.00
19.09


ATOM
4215
CZ
TYR
A
559
4.614
20.090
−51.955
1.00
18.58


ATOM
4216
OH
TYR
A
559
4.135
19.160
−51.056
1.00
20.55


ATOM
4217
CE2
TYR
A
559
3.819
20.493
−53.024
1.00
18.22


ATOM
4218
CD2
TYR
A
559
4.335
21.438
−53.946
1.00
18.82


ATOM
4219
C
TYR
A
559
8.066
21.445
−55.541
1.00
20.13


ATOM
4220
O
TYR
A
559
8.008
20.215
−55.679
1.00
20.26


ATOM
4221
N
LYS
A
560
9.098
22.079
−54.995
1.00
19.51


ATOM
4222
CA
LYS
A
560
10.208
21.379
−54.349
1.00
19.72


ATOM
4223
CB
LYS
A
560
11.410
21.175
−55.282
1.00
19.66


ATOM
4224
CG
LYS
A
560
11.390
19.870
−56.058
1.00
22.20


ATOM
4225
CD
LYS
A
560
12.767
19.633
−56.714
1.00
24.46


ATOM
4226
CE
LYS
A
560
12.781
18.341
−57.531
1.00
26.96


ATOM
4227
NZ
LYS
A
560
14.189
18.050
−57.980
1.00
26.97


ATOM
4228
C
LYS
A
560
10.680
22.257
−53.234
1.00
18.68


ATOM
4229
O
LYS
A
560
10.583
23.484
−53.318
1.00
19.68


ATOM
4230
N
TYR
A
561
11.240
21.640
−52.206
1.00
17.87


ATOM
4231
CA
TYR
A
561
11.927
22.420
−51.187
1.00
17.82


ATOM
4232
CB
TYR
A
561
11.921
21.690
−49.840
1.00
17.42


ATOM
4233
CG
TYR
A
561
10.518
21.449
−49.346
1.00
16.12


ATOM
4234
CD1
TYR
A
561
9.831
20.276
−49.661
1.00
15.94


ATOM
4235
CE1
TYR
A
561
8.511
20.062
−49.199
1.00
15.79


ATOM
4236
CZ
TYR
A
561
7.897
21.050
−48.456
1.00
15.89


ATOM
4237
OH
TYR
A
561
6.614
20.889
−47.981
1.00
17.17


ATOM
4238
CE2
TYR
A
561
8.557
22.224
−48.164
1.00
16.19


ATOM
4239
CD2
TYR
A
561
9.856
22.430
−48.625
1.00
17.57


ATOM
4240
C
TYR
A
561
13.360
22.650
−51.607
1.00
18.63


ATOM
4241
O
TYR
A
561
13.963
21.786
−52.265
1.00
18.40


ATOM
4242
N
ILE
A
562
13.904
23.792
−51.201
1.00
18.56


ATOM
4243
CA
ILE
A
562
15.322
24.085
−51.434
1.00
20.18


ATOM
4244
CB
ILE
A
562
15.524
25.247
−52.419
1.00
19.65


ATOM
4245
CG1
ILE
A
562
14.837
26.520
−51.896
1.00
21.64


ATOM
4246
CD1
ILE
A
562
15.074
27.789
−52.741
1.00
21.24


ATOM
4247
CG2
ILE
A
562
15.017
24.829
−53.797
1.00
20.44


ATOM
4248
C
ILE
A
562
15.971
24.446
−50.128
1.00
20.56


ATOM
4249
O
ILE
A
562
15.316
24.956
−49.229
1.00
19.84


ATOM
4250
N
ASN
A
563
17.254
24.134
−50.029
1.00
21.41


ATOM
4251
CA
ASN
A
563
18.076
24.467
−48.886
1.00
22.99


ATOM
4252
CB
ASN
A
563
18.833
23.209
−48.435
1.00
22.69


ATOM
4253
CG
ASN
A
563
19.629
23.433
−47.156
1.00
25.48


ATOM
4254
OD1
ASN
A
563
20.203
24.492
−46.965
1.00
28.81


ATOM
4255
ND2
ASN
A
563
19.669
22.436
−46.285
1.00
25.82


ATOM
4256
C
ASN
A
563
19.039
25.561
−49.372
1.00
24.66


ATOM
4257
O
ASN
A
563
19.794
25.326
−50.323
1.00
24.16


ATOM
4258
N
VAL
A
564
18.977
26.749
−48.780
1.00
26.20


ATOM
4259
CA
VAL
A
564
19.877
27.837
−49.206
1.00
28.74


ATOM
4260
CB
VAL
A
564
19.156
29.091
−49.832
1.00
28.91


ATOM
4261
CG1
VAL
A
564
19.461
30.408
−49.087
1.00
31.10


ATOM
4262
CG2
VAL
A
564
17.655
28.836
−50.079
1.00
28.92


ATOM
4263
C
VAL
A
564
20.886
28.181
−48.122
1.00
29.56


ATOM
4264
O
VAL
A
564
20.538
28.320
−46.954
1.00
29.10


ATOM
4265
N
GLY
A
565
22.150
28.266
−48.527
1.00
32.02


ATOM
4266
CA
GLY
A
565
23.252
28.423
−47.577
1.00
34.92


ATOM
4267
C
GLY
A
565
23.539
29.876
−47.248
1.00
37.47


ATOM
4268
O
GLY
A
565
22.969
30.788
−47.871
1.00
37.66


ATOM
4269
N
GLN
A
566
24.419
30.098
−46.267
1.00
39.85


ATOM
4270
CA
GLN
A
566
24.897
31.456
−45.926
1.00
42.75


ATOM
4271
CB
GLN
A
566
26.054
31.398
−44.918
1.00
42.83


ATOM
4272
CG
GLN
A
566
25.727
30.761
−43.565
1.00
44.99


ATOM
4273
CD
GLN
A
566
26.940
30.689
−42.626
1.00
44.88


ATOM
4274
OE1
GLN
A
566
27.972
30.089
−42.958
1.00
47.67


ATOM
4275
NE2
GLN
A
566
26.810
31.293
−41.441
1.00
47.62


ATOM
4276
C
GLN
A
566
25.373
32.195
−47.181
1.00
43.30


ATOM
4277
O
GLN
A
566
25.052
33.365
−47.389
1.00
44.01


ATOM
4278
N
ASP
A
567
26.118
31.479
−48.023
1.00
44.30


ATOM
4279
CA
ASP
A
567
26.739
32.029
−49.236
1.00
44.62


ATOM
4280
CB
ASP
A
567
27.916
31.139
−49.650
1.00
45.13


ATOM
4281
CG
ASP
A
567
27.492
29.702
−49.966
1.00
47.62


ATOM
4282
OD1
ASP
A
567
26.421
29.255
−49.485
1.00
48.73


ATOM
4283
OD2
ASP
A
567
28.245
29.010
−50.693
1.00
50.47


ATOM
4284
C
ASP
A
567
25.776
32.197
−50.421
1.00
44.04


ATOM
4285
O
ASP
A
567
26.196
32.575
−51.522
1.00
44.36


ATOM
4286
N
GLY
A
568
24.497
31.899
−50.205
1.00
42.85


ATOM
4287
CA
GLY
A
568
23.488
32.045
−51.247
1.00
41.38


ATOM
4288
C
GLY
A
568
23.359
30.851
−52.177
1.00
40.41


ATOM
4289
O
GLY
A
568
22.496
30.854
−53.054
1.00
40.62


ATOM
4290
N
SER
A
569
24.195
29.827
−51.990
1.00
39.02


ATOM
4291
CA
SER
A
569
24.137
28.623
−52.827
1.00
37.72


ATOM
4292
CB
SER
A
569
25.365
27.746
−52.600
1.00
38.07


ATOM
4293
OG
SER
A
569
25.454
27.359
−51.238
1.00
39.18


ATOM
4294
C
SER
A
569
22.868
27.819
−52.540
1.00
36.72


ATOM
4295
O
SER
A
569
22.474
27.672
−51.382
1.00
36.47


ATOM
4296
N
VAL
A
570
22.222
27.313
−53.583
1.00
35.25


ATOM
4297
CA
VAL
A
570
20.988
26.558
−53.365
1.00
34.26


ATOM
4298
CB
VAL
A
570
19.709
27.243
−53.987
1.00
34.42


ATOM
4299
CG1
VAL
A
570
18.992
26.358
−55.020
1.00
35.11


ATOM
4300
CG2
VAL
A
570
20.010
28.651
−54.506
1.00
35.12


ATOM
4301
C
VAL
A
570
21.113
25.080
−53.718
1.00
33.29


ATOM
4302
O
VAL
A
570
21.735
24.694
−54.714
1.00
32.88


ATOM
4303
N
THR
A
571
20.515
24.261
−52.864
1.00
31.66


ATOM
4304
CA
THR
A
571
20.480
22.825
−53.021
1.00
30.87


ATOM
4305
CB
THR
A
571
21.016
22.146
−51.752
1.00
30.96


ATOM
4306
OG1
THR
A
571
22.311
22.686
−51.442
1.00
33.18


ATOM
4307
CG2
THR
A
571
21.117
20.637
−51.935
1.00
30.95


ATOM
4308
C
THR
A
571
19.018
22.473
−53.210
1.00
29.72


ATOM
4309
O
THR
A
571
18.186
22.832
−52.373
1.00
28.84


ATOM
4310
N
TRP
A
572
18.697
21.846
−54.337
1.00
29.01


ATOM
4311
CA
TRP
A
572
17.331
21.388
−54.589
1.00
28.49


ATOM
4312
CB
TRP
A
572
17.004
21.367
−56.086
1.00
28.99


ATOM
4313
CG
TRP
A
572
16.950
22.690
−56.739
1.00
29.65


ATOM
4314
CD1
TRP
A
572
18.014
23.419
−57.217
1.00
30.67


ATOM
4315
NE1
TRP
A
572
17.564
24.603
−57.769
1.00
31.84


ATOM
4316
CE2
TRP
A
572
16.196
24.655
−57.668
1.00
30.45


ATOM
4317
CD2
TRP
A
572
15.770
23.464
−57.028
1.00
30.30


ATOM
4318
CE3
TRP
A
572
14.398
23.274
−56.791
1.00
29.43


ATOM
4319
CZ3
TRP
A
572
13.502
24.266
−57.205
1.00
30.24


ATOM
4320
CH2
TRP
A
572
13.959
25.437
−57.846
1.00
30.31


ATOM
4321
CZ2
TRP
A
572
15.298
25.649
−58.083
1.00
29.71


ATOM
4322
C
TRP
A
572
17.205
19.991
−54.031
1.00
28.21


ATOM
4323
O
TRP
A
572
18.168
19.212
−54.060
1.00
27.50


ATOM
4324
N
GLU
A
573
16.033
19.647
−53.499
1.00
27.69


ATOM
4325
CA
GLU
A
573
15.819
18.251
−53.123
1.00
27.17


ATOM
4326
CB
GLU
A
573
14.586
18.074
−52.222
1.00
27.18


ATOM
4327
CG
GLU
A
573
13.287
18.406
−52.901
1.00
26.04


ATOM
4328
CD
GLU
A
573
12.059
18.111
−52.028
1.00
26.20


ATOM
4329
OE1
GLU
A
573
12.112
17.224
−51.141
1.00
25.84


ATOM
4330
OE2
GLU
A
573
11.032
18.764
−52.264
1.00
22.24


ATOM
4331
C
GLU
A
573
15.725
17.419
−54.405
1.00
27.91


ATOM
4332
O
GLU
A
573
15.498
17.957
−55.497
1.00
26.94


ATOM
4333
N
SER
A
574
15.907
16.108
−54.267
1.00
28.48


ATOM
4334
CA
SER
A
574
15.880
15.201
−55.410
1.00
29.59


ATOM
4335
CB
SER
A
574
16.296
13.805
−54.975
1.00
29.73


ATOM
4336
OG
SER
A
574
17.609
13.875
−54.449
1.00
32.74


ATOM
4337
C
SER
A
574
14.526
15.134
−56.095
1.00
29.70


ATOM
4338
O
SER
A
574
13.500
15.482
−55.513
1.00
29.12


ATOM
4339
N
ASP
A
575
14.544
14.669
−57.339
1.00
29.54


ATOM
4340
CA
ASP
A
575
13.337
14.435
−58.109
1.00
29.98


ATOM
4341
CB
ASP
A
575
13.705
14.000
−59.534
1.00
30.69


ATOM
4342
CG
ASP
A
575
14.324
15.125
−60.331
1.00
33.96


ATOM
4343
OD1
ASP
A
575
14.056
16.299
−59.997
1.00
36.25


ATOM
4344
OD2
ASP
A
575
15.083
14.846
−61.290
1.00
37.94


ATOM
4345
C
ASP
A
575
12.519
13.358
−57.428
1.00
28.93


ATOM
4346
O
ASP
A
575
13.050
12.633
−56.600
1.00
28.34


ATOM
4347
N
PRO
A
576
11.217
13.267
−57.760
1.00
28.58


ATOM
4348
CA
PRO
A
576
10.469
14.173
−58.650
1.00
27.92


ATOM
4349
CB
PRO
A
576
9.319
13.294
−59.131
1.00
28.52


ATOM
4350
CG
PRO
A
576
9.053
12.378
−57.954
1.00
28.29


ATOM
4351
CD
PRO
A
576
10.377
12.159
−57.267
1.00
28.76


ATOM
4352
C
PRO
A
576
9.894
15.397
−57.938
1.00
27.25


ATOM
4353
O
PRO
A
576
9.887
15.452
−56.703
1.00
28.13


ATOM
4354
N
ASN
A
577
9.394
16.360
−58.707
1.00
25.62


ATOM
4355
CA
ASN
A
577
8.612
17.449
−58.129
1.00
24.83


ATOM
4356
CB
ASN
A
577
8.013
18.336
−59.224
1.00
24.90


ATOM
4357
CG
ASN
A
577
9.055
19.184
−59.913
1.00
25.61


ATOM
4358
OD1
ASN
A
577
10.176
19.321
−59.423
1.00
25.83


ATOM
4359
ND2
ASN
A
577
8.693
19.756
−61.060
1.00
25.07


ATOM
4360
C
ASN
A
577
7.466
16.868
−57.322
1.00
24.22


ATOM
4361
O
ASN
A
577
6.949
15.798
−57.672
1.00
23.69


ATOM
4362
N
HIS
A
578
7.057
17.562
−56.259
1.00
23.20


ATOM
4363
CA
HIS
A
578
5.830
17.179
−55.570
1.00
22.96


ATOM
4364
CB
HIS
A
578
5.734
17.844
−54.200
1.00
22.09


ATOM
4365
CG
HIS
A
578
6.874
17.538
−53.285
1.00
21.80


ATOM
4366
ND1
HIS
A
578
6.809
16.558
−52.318
1.00
21.62


ATOM
4367
CE1
HIS
A
578
7.948
16.530
−51.645
1.00
21.36


ATOM
4368
NE2
HIS
A
578
8.743
17.465
−52.133
1.00
20.16


ATOM
4369
CD2
HIS
A
578
8.096
18.109
−53.160
1.00
19.78


ATOM
4370
C
HIS
A
578
4.697
17.707
−56.429
1.00
23.58


ATOM
4371
O
HIS
A
578
4.814
18.794
−56.976
1.00
23.64


ATOM
4372
N
THR
A
579
3.603
16.955
−56.534
1.00
23.98


ATOM
4373
CA
THR
A
579
2.426
17.448
−57.254
1.00
25.34


ATOM
4374
CB
THR
A
579
2.092
16.568
−58.477
1.00
26.08


ATOM
4375
OG1
THR
A
579
3.162
16.672
−59.429
1.00
29.14


ATOM
4376
CG2
THR
A
579
0.749
16.979
−59.126
1.00
26.14


ATOM
4377
C
THR
A
579
1.259
17.480
−56.291
1.00
25.05


ATOM
4378
O
THR
A
579
0.977
16.487
−55.629
1.00
25.73


ATOM
4379
N
TYR
A
580
0.591
18.619
−56.213
1.00
25.23


ATOM
4380
CA
TYR
A
580
−0.450
18.802
−55.211
1.00
25.68


ATOM
4381
CB
TYR
A
580
0.098
19.556
−53.976
1.00
25.88


ATOM
4382
CG
TYR
A
580
−0.931
19.763
−52.866
1.00
26.21


ATOM
4383
CD1
TYR
A
580
−1.284
21.048
−52.429
1.00
26.22


ATOM
4384
CE1
TYR
A
580
−2.256
21.233
−51.399
1.00
27.53


ATOM
4385
CZ
TYR
A
580
−2.860
20.111
−50.841
1.00
27.08


ATOM
4386
OH
TYR
A
580
−3.806
20.207
−49.844
1.00
27.88


ATOM
4387
CE2
TYR
A
580
−2.510
18.841
−51.264
1.00
26.97


ATOM
4388
CD2
TYR
A
580
−1.562
18.671
−52.276
1.00
26.29


ATOM
4389
C
TYR
A
580
−1.634
19.523
−55.828
1.00
25.65


ATOM
4390
O
TYR
A
580
−1.490
20.596
−56.403
1.00
25.29


ATOM
4391
N
THR
A
581
−2.813
18.915
−55.732
1.00
25.95


ATOM
4392
CA
THR
A
581
−4.015
19.629
−56.117
1.00
25.78


ATOM
4393
CB
THR
A
581
−5.016
18.700
−56.806
1.00
26.75


ATOM
4394
OG1
THR
A
581
−4.332
18.000
−57.855
1.00
26.57


ATOM
4395
CG2
THR
A
581
−6.189
19.498
−57.397
1.00
27.62


ATOM
4396
C
THR
A
581
−4.627
20.285
−54.874
1.00
25.36


ATOM
4397
O
THR
A
581
−5.024
19.595
−53.935
1.00
25.22


ATOM
4398
N
VAL
A
582
−4.685
21.615
−54.880
1.00
24.54


ATOM
4399
CA
VAL
A
582
−5.255
22.382
−53.777
1.00
24.15


ATOM
4400
CB
VAL
A
582
−5.006
23.915
−53.953
1.00
24.21


ATOM
4401
CG1
VAL
A
582
−5.472
24.700
−52.744
1.00
23.87


ATOM
4402
CG2
VAL
A
582
−3.514
24.218
−54.219
1.00
25.33


ATOM
4403
C
VAL
A
582
−6.759
22.063
−53.706
1.00
24.32


ATOM
4404
O
VAL
A
582
−7.478
22.204
−54.700
1.00
23.26


ATOM
4405
N
PRO
A
583
−7.236
21.587
−52.546
1.00
24.10


ATOM
4406
CA
PRO
A
583
−8.665
21.230
−52.476
1.00
24.34


ATOM
4407
CB
PRO
A
583
−8.865
20.763
−51.022
1.00
24.33


ATOM
4408
CG
PRO
A
583
−7.516
20.538
−50.468
1.00
25.16


ATOM
4409
CD
PRO
A
583
−6.508
21.310
−51.294
1.00
24.72


ATOM
4410
C
PRO
A
583
−9.597
22.404
−52.768
1.00
24.50


ATOM
4411
O
PRO
A
583
−9.262
23.558
−52.487
1.00
23.93


ATOM
4412
N
ALA
A
584
−10.756
22.104
−53.350
1.00
24.61


ATOM
4413
CA
ALA
A
584
−11.817
23.084
−53.477
1.00
24.77


ATOM
4414
CB
ALA
A
584
−12.065
23.439
−54.943
1.00
25.29


ATOM
4415
C
ALA
A
584
−13.036
22.434
−52.847
1.00
24.94


ATOM
4416
O
ALA
A
584
−13.922
21.932
−53.537
1.00
25.03


ATOM
4417
N
VAL
A
585
−13.052
22.406
−51.517
1.00
24.24


ATOM
4418
CA
VAL
A
585
−14.075
21.673
−50.776
1.00
23.75


ATOM
4419
CB
VAL
A
585
−13.465
20.452
−50.029
1.00
24.50


ATOM
4420
CG1
VAL
A
585
−14.515
19.770
−49.151
1.00
24.48


ATOM
4421
CG2
VAL
A
585
−12.863
19.447
−51.026
1.00
25.32


ATOM
4422
C
VAL
A
585
−14.707
22.639
−49.781
1.00
23.20


ATOM
4423
O
VAL
A
585
−13.999
23.347
−49.065
1.00
22.13


ATOM
4424
N
ALA
A
586
−16.044
22.679
−49.739
1.00
22.43


ATOM
4425
CA
ALA
A
586
−16.749
23.546
−48.804
1.00
21.79


ATOM
4426
CB
ALA
A
586
−18.240
23.212
−48.820
1.00
22.19


ATOM
4427
C
ALA
A
586
−16.160
23.324
−47.389
1.00
21.57


ATOM
4428
O
ALA
A
586
−15.954
22.180
−46.990
1.00
20.89


ATOM
4429
N
CYS
A
587
−15.872
24.414
−46.679
1.00
21.59


ATOM
4430
CA
CYS
A
587
−15.388
24.379
−45.268
1.00
21.68


ATOM
4431
CB
CYS
A
587
−16.131
23.323
−44.441
1.00
22.08


ATOM
4432
SG
CYS
A
587
−17.952
23.374
−44.507
1.00
23.60


ATOM
4433
C
CYS
A
587
−13.886
24.129
−45.094
1.00
21.27


ATOM
4434
O
CYS
A
587
−13.386
24.225
−43.980
1.00
21.08


ATOM
4435
N
VAL
A
588
−13.178
23.780
−46.170
1.00
20.53


ATOM
4436
CA
VAL
A
588
−11.742
23.499
−46.085
1.00
20.52


ATOM
4437
CB
VAL
A
588
−11.351
22.268
−46.958
1.00
20.47


ATOM
4438
CG1
VAL
A
588
−9.846
21.959
−46.844
1.00
20.87


ATOM
4439
CG2
VAL
A
588
−12.163
21.042
−46.549
1.00
20.51


ATOM
4440
C
VAL
A
588
−10.949
24.731
−46.504
1.00
20.59


ATOM
4441
O
VAL
A
588
−10.699
24.950
−47.705
1.00
21.88


ATOM
4442
N
THR
A
589
−10.533
25.522
−45.528
1.00
19.56


ATOM
4443
CA
THR
A
589
−9.903
26.807
−45.795
1.00
19.48


ATOM
4444
CB
THR
A
589
−10.595
27.914
−44.988
1.00
20.13


ATOM
4445
OG1
THR
A
589
−10.527
27.565
−43.592
1.00
21.49


ATOM
4446
CG2
THR
A
589
−12.085
28.018
−45.410
1.00
20.03


ATOM
4447
C
THR
A
589
−8.424
26.819
−45.427
1.00
19.42


ATOM
4448
O
THR
A
589
−7.694
27.767
−45.743
1.00
18.66


ATOM
4449
N
GLN
A
590
−7.995
25.772
−44.734
1.00
19.62


ATOM
4450
CA
GLN
A
590
−6.606
25.629
−44.317
1.00
20.20


ATOM
4451
CB
GLN
A
590
−6.359
26.261
−42.939
1.00
21.06


ATOM
4452
CG
GLN
A
590
−4.950
25.956
−42.410
1.00
27.00


ATOM
4453
CD
GLN
A
590
−4.184
27.189
−41.989
1.00
33.74


ATOM
4454
OE1
GLN
A
590
−4.771
28.196
−41.611
1.00
37.22


ATOM
4455
NE2
GLN
A
590
−2.855
27.118
−42.066
1.00
36.77


ATOM
4456
C
GLN
A
590
−6.247
24.159
−44.295
1.00
19.00


ATOM
4457
O
GLN
A
590
−7.004
23.335
−43.771
1.00
18.70


ATOM
4458
N
VAL
A
591
−5.113
23.811
−44.904
1.00
17.70


ATOM
4459
CA
VAL
A
591
−4.682
22.404
−44.940
1.00
17.15


ATOM
4460
CB
VAL
A
591
−4.843
21.750
−46.330
1.00
17.82


ATOM
4461
CG1
VAL
A
591
−6.316
21.701
−46.744
1.00
18.11


ATOM
4462
CG2
VAL
A
591
−3.970
22.470
−47.390
1.00
17.60


ATOM
4463
C
VAL
A
591
−3.213
22.360
−44.551
1.00
17.42


ATOM
4464
O
VAL
A
591
−2.531
23.377
−44.638
1.00
17.32


ATOM
4465
N
VAL
A
592
−2.731
21.206
−44.090
1.00
17.26


ATOM
4466
CA
VAL
A
592
−1.291
21.092
−43.887
1.00
17.03


ATOM
4467
CB
VAL
A
592
−0.762
21.198
−42.365
1.00
18.70


ATOM
4468
CG1
VAL
A
592
0.335
20.217
−41.930
1.00
18.75


ATOM
4469
CG2
VAL
A
592
−1.810
21.731
−41.315
1.00
15.36


ATOM
4470
C
VAL
A
592
−0.736
19.951
−44.730
1.00
16.99


ATOM
4471
O
VAL
A
592
−1.318
18.862
−44.828
1.00
16.23


ATOM
4472
N
LYS
A
593
0.357
20.253
−45.403
1.00
15.38


ATOM
4473
CA
LYS
A
593
0.953
19.302
−46.293
1.00
16.27


ATOM
4474
CB
LYS
A
593
1.301
20.010
−47.616
1.00
16.69


ATOM
4475
CG
LYS
A
593
1.835
19.096
−48.694
1.00
20.55


ATOM
4476
CD
LYS
A
593
0.791
18.101
−49.203
1.00
24.73


ATOM
4477
CE
LYS
A
593
1.330
17.311
−50.409
1.00
27.26


ATOM
4478
NZ
LYS
A
593
2.395
16.299
−50.074
1.00
28.37


ATOM
4479
C
LYS
A
593
2.209
18.783
−45.588
1.00
16.01


ATOM
4480
O
LYS
A
593
3.175
19.525
−45.427
1.00
15.12


ATOM
4481
N
GLU
A
594
2.195
17.519
−45.175
1.00
15.82


ATOM
4482
CA
GLU
A
594
3.308
16.969
−44.407
1.00
16.23


ATOM
4483
CB
GLU
A
594
2.798
16.017
−43.317
1.00
16.26


ATOM
4484
CG
GLU
A
594
1.866
16.732
−42.299
1.00
16.62


ATOM
4485
CD
GLU
A
594
1.727
15.949
−40.991
1.00
18.94


ATOM
4486
OE1
GLU
A
594
1.267
14.778
−41.024
1.00
21.31


ATOM
4487
OE2
GLU
A
594
2.107
16.507
−39.940
1.00
16.43


ATOM
4488
C
GLU
A
594
4.286
16.245
−45.323
1.00
17.34


ATOM
4489
O
GLU
A
594
3.973
15.177
−45.852
1.00
17.78


ATOM
4490
N
ASP
A
595
5.463
16.833
−45.487
1.00
17.05


ATOM
4491
CA
ASP
A
595
6.481
16.326
−46.405
1.00
17.21


ATOM
4492
CB
ASP
A
595
6.823
17.379
−47.475
1.00
16.63


ATOM
4493
CG
ASP
A
595
5.678
17.619
−48.455
1.00
17.91


ATOM
4494
OD1
ASP
A
595
5.023
16.631
−48.857
1.00
20.73


ATOM
4495
OD2
ASP
A
595
5.434
18.795
−48.844
1.00
18.08


ATOM
4496
C
ASP
A
595
7.734
15.955
−45.631
1.00
17.47


ATOM
4497
O
ASP
A
595
7.915
16.375
−44.492
1.00
16.44


ATOM
4498
N
THR
A
596
8.598
15.162
−46.277
1.00
18.11


ATOM
4499
CA
THR
A
596
9.917
14.835
−45.747
1.00
18.85


ATOM
4500
CB
THR
A
596
9.991
13.390
−45.188
1.00
19.58


ATOM
4501
OG1
THR
A
596
9.057
13.248
−44.116
1.00
20.97


ATOM
4502
CG2
THR
A
596
11.385
13.124
−44.598
1.00
20.94


ATOM
4503
C
THR
A
596
10.895
14.978
−46.914
1.00
19.34


ATOM
4504
O
THR
A
596
10.588
14.531
−48.024
1.00
19.05


ATOM
4505
N
TRP
A
597
12.050
15.581
−46.631
1.00
20.24


ATOM
4506
CA
TRP
A
597
13.074
15.921
−47.633
1.00
21.94


ATOM
4507
CB
TRP
A
597
14.325
16.453
−46.940
1.00
22.34


ATOM
4508
CG
TRP
A
597
15.445
16.854
−47.882
1.00
23.75


ATOM
4509
CD1
TRP
A
597
16.509
16.079
−48.275
1.00
25.08


ATOM
4510
NE1
TRP
A
597
17.327
16.801
−49.138
1.00
24.85


ATOM
4511
CE2
TRP
A
597
16.802
18.059
−49.300
1.00
25.25


ATOM
4512
CD2
TRP
A
597
15.611
18.128
−48.527
1.00
24.09


ATOM
4513
CE3
TRP
A
597
14.875
19.325
−48.519
1.00
24.91


ATOM
4514
CZ3
TRP
A
597
15.334
20.401
−49.262
1.00
23.38


ATOM
4515
CH2
TRP
A
597
16.520
20.299
−50.028
1.00
25.14


ATOM
4516
CZ2
TRP
A
597
17.265
19.137
−50.053
1.00
22.49


ATOM
4517
C
TRP
A
597
13.424
14.708
−48.473
1.00
23.42


ATOM
4518
O
TRP
A
597
13.675
13.635
−47.939
1.00
22.37


ATOM
4519
N
GLN
A
598
13.409
14.904
−49.788
1.00
25.26


ATOM
4520
CA
GLN
A
598
13.698
13.850
−50.755
1.00
27.05


ATOM
4521
CB
GLN
A
598
12.936
14.124
−52.052
1.00
26.51


ATOM
4522
CG
GLN
A
598
11.418
13.948
−51.895
1.00
26.10


ATOM
4523
CD
GLN
A
598
10.642
14.209
−53.156
1.00
26.76


ATOM
4524
OE1
GLN
A
598
11.194
14.620
−54.175
1.00
27.68


ATOM
4525
NE2
GLN
A
598
9.340
13.990
−53.095
1.00
25.96


ATOM
4526
C
GLN
A
598
15.204
13.787
−50.977
1.00
29.21


ATOM
4527
O
GLN
A
598
15.794
14.694
−51.574
1.00
28.61


ATOM
4528
N
SER
A
599
15.818
12.722
−50.453
1.00
32.41


ATOM
4529
CA
SER
A
599
17.273
12.530
−50.498
1.00
35.70


ATOM
4530
CB
SER
A
599
17.747
11.698
−49.302
1.00
35.61


ATOM
4531
OG
SER
A
599
17.374
12.296
−48.072
1.00
39.62


ATOM
4532
C
SER
A
599
17.703
11.831
−51.785
1.00
36.66


ATOM
4533
O
SER
A
599
16.916
11.145
−52.433
1.00
37.44


ATOM
4534
OXT
SER
A
599
18.863
11.922
−52.194
1.00
38.18


ATOM
4535
C1
MAN
A
601
−3.602
−3.018
−46.412
1.00
102.64


ATOM
4536
C2
MAN
A
601
−4.584
−2.109
−47.156
1.00
102.73


ATOM
4537
O2
MAN
A
601
−3.951
−1.548
−48.288
1.00
102.91


ATOM
4538
C3
MAN
A
601
−5.867
−2.845
−47.570
1.00
102.38


ATOM
4539
O3
MAN
A
601
−6.544
−2.112
−48.566
1.00
102.32


ATOM
4540
C4
MAN
A
601
−5.640
−4.269
−48.082
1.00
102.18


ATOM
4541
O4
MAN
A
601
−6.860
−4.967
−47.984
1.00
101.76


ATOM
4542
C5
MAN
A
601
−4.561
−5.018
−47.298
1.00
102.40


ATOM
4543
C6
MAN
A
601
−4.172
−6.307
−48.010
1.00
102.48


ATOM
4544
O6
MAN
A
601
−3.156
−6.957
−47.280
1.00
102.80


ATOM
4545
O5
MAN
A
601
−3.400
−4.222
−47.131
1.00
102.71


ATOM
4546
C1
MAN
A
602
−29.428
−4.974
−42.477
1.00
77.32


ATOM
4547
C2
MAN
A
602
−28.973
−6.434
−42.405
1.00
77.44


ATOM
4548
O2
MAN
A
602
−30.120
−7.253
−42.347
1.00
77.72


ATOM
4549
C3
MAN
A
602
−28.044
−6.835
−43.565
1.00
77.20


ATOM
4550
O3
MAN
A
602
−27.940
−8.239
−43.664
1.00
76.96


ATOM
4551
C4
MAN
A
602
−28.487
−6.260
−44.909
1.00
77.22


ATOM
4552
O4
MAN
A
602
−27.471
−6.474
−45.862
1.00
76.90


ATOM
4553
C5
MAN
A
602
−28.766
−4.766
−44.768
1.00
77.47


ATOM
4554
C6
MAN
A
602
−29.185
−4.115
−46.081
1.00
77.84


ATOM
4555
O6
MAN
A
602
−28.163
−3.228
−46.483
1.00
78.24


ATOM
4556
O5
MAN
A
602
−29.768
−4.562
−43.790
1.00
77.46


ATOM
4557
C1
MAN
A
603
−18.689
25.235
−53.677
1.00
47.04


ATOM
4558
C2
MAN
A
603
−20.074
24.872
−53.114
1.00
51.09


ATOM
4559
O2
MAN
A
603
−21.044
25.065
−54.120
1.00
52.42


ATOM
4560
C3
MAN
A
603
−20.141
23.420
−52.620
1.00
51.78


ATOM
4561
O3
MAN
A
603
−21.465
23.079
−52.262
1.00
53.36


ATOM
4562
C4
MAN
A
603
−19.602
22.466
−53.686
1.00
51.72


ATOM
4563
O4
MAN
A
603
−19.615
21.142
−53.209
1.00
51.94


ATOM
4564
C5
MAN
A
603
−18.179
22.911
−54.021
1.00
51.66


ATOM
4565
C6
MAN
A
603
−17.421
21.906
−54.892
1.00
53.80


ATOM
4566
O6
MAN
A
603
−17.915
21.885
−56.214
1.00
55.49


ATOM
4567
O5
MAN
A
603
−18.217
24.223
−54.581
1.00
49.62


ATOM
4568
C1
MAN
A
605
−4.678
15.117
−57.896
1.00
58.79


ATOM
4569
C2
MAN
A
605
−3.360
15.555
−58.538
1.00
58.65


ATOM
4570
O2
MAN
A
605
−2.564
14.412
−58.722
1.00
59.38


ATOM
4571
C3
MAN
A
605
−3.570
16.269
−59.878
1.00
58.70


ATOM
4572
O3
MAN
A
605
−2.523
15.985
−60.778
1.00
59.27


ATOM
4573
C4
MAN
A
605
−4.915
15.892
−60.491
1.00
58.73


ATOM
4574
O4
MAN
A
605
−5.084
16.538
−61.730
1.00
59.62


ATOM
4575
C5
MAN
A
605
−6.054
16.284
−59.547
1.00
58.90


ATOM
4576
C6
MAN
A
605
−7.370
15.612
−59.932
1.00
58.75


ATOM
4577
O6
MAN
A
605
−7.255
14.219
−59.738
1.00
59.42


ATOM
4578
O5
MAN
A
605
−5.730
16.034
−58.173
1.00
58.30


ATOM
4579
C1
MAN
A
606
−10.273
28.688
−42.727
1.00
25.90


ATOM
4580
C2
MAN
A
606
−9.839
27.944
−41.452
1.00
28.64


ATOM
4581
O2
MAN
A
606
−9.245
28.909
−40.620
1.00
28.84


ATOM
4582
C3
MAN
A
606
−10.999
27.249
−40.710
1.00
29.65


ATOM
4583
O3
MAN
A
606
−10.568
26.763
−39.441
1.00
28.85


ATOM
4584
C4
MAN
A
606
−12.203
28.177
−40.551
1.00
30.36


ATOM
4585
O4
MAN
A
606
−13.330
27.463
−40.084
1.00
30.29


ATOM
4586
C5
MAN
A
606
−12.553
28.769
−41.914
1.00
30.72


ATOM
4587
C6
MAN
A
606
−13.730
29.731
−41.778
1.00
33.97


ATOM
4588
O6
MAN
A
606
−13.624
30.732
−42.762
1.00
36.82


ATOM
4589
O5
MAN
A
606
−11.434
29.464
−42.435
1.00
28.12


ATOM
4590
C1
MAN
A
607
−31.396
1.963
−40.521
1.00
50.29


ATOM
4591
C2
MAN
A
607
−30.220
1.790
−41.485
1.00
52.65


ATOM
4592
O2
MAN
A
607
−30.541
0.785
−42.419
1.00
54.93


ATOM
4593
C3
MAN
A
607
−29.845
3.092
−42.208
1.00
52.48


ATOM
4594
O3
MAN
A
607
−28.932
2.836
−43.251
1.00
53.01


ATOM
4595
C4
MAN
A
607
−31.068
3.818
−42.766
1.00
52.78


ATOM
4596
O4
MAN
A
607
−30.672
5.070
−43.297
1.00
52.92


ATOM
4597
C5
MAN
A
607
−32.103
3.985
−41.652
1.00
52.23


ATOM
4598
C6
MAN
A
607
−33.331
4.749
−42.153
1.00
52.96


ATOM
4599
O6
MAN
A
607
−34.520
4.076
−41.791
1.00
52.95


ATOM
4600
O5
MAN
A
607
−32.451
2.702
−41.127
1.00
51.79


ATOM
4601
C1
MAN
A
608
3.870
15.416
−59.489
1.00
37.21


ATOM
4602
C2
MAN
A
608
5.134
15.938
−60.168
1.00
40.45


ATOM
4603
O2
MAN
A
608
6.091
14.903
−60.120
1.00
38.47


ATOM
4604
C3
MAN
A
608
4.872
16.381
−61.608
1.00
42.66


ATOM
4605
O3
MAN
A
608
6.071
16.726
−62.263
1.00
44.20


ATOM
4606
C4
MAN
A
608
4.122
15.321
−62.401
1.00
44.80


ATOM
4607
O4
MAN
A
608
3.708
15.907
−63.612
1.00
47.73


ATOM
4608
C5
MAN
A
608
2.893
14.887
−61.597
1.00
44.80


ATOM
4609
C6
MAN
A
608
2.042
13.861
−62.342
1.00
47.55


ATOM
4610
O6
MAN
A
608
1.085
14.582
−63.104
1.00
49.87


ATOM
4611
O5
MAN
A
608
3.262
14.423
−60.302
1.00
42.18


ATOM
4612
C1
NAG
A
611
3.450
−2.354
−8.282
1.00
23.44


ATOM
4613
C2
NAG
A
611
3.474
−0.875
−7.878
1.00
24.51


ATOM
4614
N2
NAG
A
611
4.425
−0.077
−8.630
1.00
21.95


ATOM
4615
C7
NAG
A
611
4.123
0.454
−9.818
1.00
22.94


ATOM
4616
O7
NAG
A
611
3.030
0.322
−10.367
1.00
20.93


ATOM
4617
C8
NAG
A
611
5.216
1.232
−10.481
1.00
21.54


ATOM
4618
C3
NAG
A
611
3.741
−0.713
−6.380
1.00
25.60


ATOM
4619
O3
NAG
A
611
3.676
0.655
−6.047
1.00
24.91


ATOM
4620
C4
NAG
A
611
2.741
−1.528
−5.554
1.00
25.70


ATOM
4621
O4
NAG
A
611
3.196
−1.598
−4.227
1.00
28.27


ATOM
4622
C5
NAG
A
611
2.648
−2.952
−6.086
1.00
26.18


ATOM
4623
C6
NAG
A
611
1.524
−3.738
−5.397
1.00
26.64


ATOM
4624
O6
NAG
A
611
0.278
−3.081
−5.497
1.00
25.38


ATOM
4625
O5
NAG
A
611
2.437
−2.975
−7.488
1.00
24.34


ATOM
4626
C1
NAG
A
612
2.499
−0.713
−3.326
1.00
32.04


ATOM
4627
C2
NAG
A
612
2.710
−1.192
−1.879
1.00
35.81


ATOM
4628
N2
NAG
A
612
2.254
−2.556
−1.666
1.00
37.89


ATOM
4629
C7
NAG
A
612
3.072
−3.605
−1.753
1.00
39.19


ATOM
4630
O7
NAG
A
612
4.277
−3.517
−2.031
1.00
40.58


ATOM
4631
C8
NAG
A
612
2.439
−4.947
−1.507
1.00
38.98


ATOM
4632
C3
NAG
A
612
2.012
−0.256
−0.899
1.00
37.96


ATOM
4633
O3
NAG
A
612
2.352
−0.666
0.403
1.00
41.23


ATOM
4634
C4
NAG
A
612
2.491
1.176
−1.129
1.00
37.63


ATOM
4635
O4
NAG
A
612
1.789
2.053
−0.278
1.00
40.85


ATOM
4636
C5
NAG
A
612
2.294
1.565
−2.604
1.00
35.10


ATOM
4637
C6
NAG
A
612
2.785
2.982
−2.903
1.00
31.93


ATOM
4638
O6
NAG
A
612
4.188
2.994
−3.008
0.58
32.70


ATOM
4639
O5
NAG
A
612
2.974
0.625
−3.425
1.00
31.95


ATOM
4640
O8
BTB
A
620
−1.213
18.638
−21.639
1.00
23.78


ATOM
4641
C8
BTB
A
620
−1.255
19.440
−22.838
1.00
17.50


ATOM
4642
C7
BTB
A
620
−2.257
18.851
−23.831
1.00
15.39


ATOM
4643
N
BTB
A
620
−1.808
17.505
−24.294
1.00
13.88


ATOM
4644
C5
BTB
A
620
−1.274
17.600
−25.684
1.00
12.99


ATOM
4645
C6
BTB
A
620
0.017
18.399
−25.786
1.00
14.67


ATOM
4646
O6
BTB
A
620
0.949
18.004
−24.768
1.00
16.93


ATOM
4647
C2
BTB
A
620
−2.926
16.495
−24.191
1.00
13.33


ATOM
4648
C4
BTB
A
620
−4.238
16.972
−24.835
1.00
13.45


ATOM
4649
O4
BTB
A
620
−4.167
17.018
−26.265
1.00
14.77


ATOM
4650
C3
BTB
A
620
−3.213
16.295
−22.703
1.00
13.18


ATOM
4651
O3
BTB
A
620
−1.984
15.920
−22.059
1.00
12.74


ATOM
4652
C1
BTB
A
620
−2.501
15.161
−24.845
1.00
13.57


ATOM
4653
O1
BTB
A
620
−3.463
14.138
−24.525
1.00
13.07


ATOM
4654
O
WAT
W
1
−7.741
16.530
−28.587
1.00
12.90


ATOM
4655
O
WAT
W
2
−1.955
18.721
−7.814
1.00
11.77


ATOM
4656
O
WAT
W
3
−17.101
16.033
−19.836
1.00
15.26


ATOM
4657
O
WAT
W
4
−1.389
7.464
−24.070
1.00
15.86


ATOM
4658
O
WAT
W
5
−8.070
20.758
−43.462
1.00
19.56


ATOM
4659
O
WAT
W
6
−12.959
28.534
−26.860
1.00
16.12


ATOM
4660
O
WAT
W
7
−0.502
31.488
−57.004
1.00
33.06


ATOM
4661
O
WAT
W
8
2.095
5.710
−17.808
1.00
18.68


ATOM
4662
O
WAT
W
9
−7.601
14.567
−6.827
1.00
14.97


ATOM
4663
O
WAT
W
10
24.863
23.325
−37.431
1.00
32.31


ATOM
4664
O
WAT
W
11
−22.569
7.289
−10.357
1.00
17.52


ATOM
4665
O
WAT
W
12
−18.987
1.758
−22.078
1.00
23.03


ATOM
4666
O
WAT
W
13
−3.226
16.264
−54.338
1.00
32.98


ATOM
4667
O
WAT
W
14
6.141
16.546
−42.196
1.00
16.00


ATOM
4668
O
WAT
W
15
−10.356
21.827
−22.675
1.00
13.52


ATOM
4669
O
WAT
W
16
−3.130
25.355
−17.925
1.00
14.01


ATOM
4670
O
WAT
W
17
−11.823
29.479
−29.411
1.00
17.50


ATOM
4671
O
WAT
W
18
−14.383
15.964
−19.553
1.00
13.02


ATOM
4672
O
WAT
W
19
−1.180
16.935
−10.101
1.00
18.86


ATOM
4673
O
WAT
W
20
−31.133
23.501
4.462
1.00
16.66


ATOM
4674
O
WAT
W
21
−4.819
24.193
−15.023
1.00
14.31


ATOM
4675
O
WAT
W
22
1.709
22.276
−4.126
1.00
21.96


ATOM
4676
O
WAT
W
23
−5.339
21.386
−7.463
1.00
15.78


ATOM
4677
O
WAT
W
24
−17.232
15.476
1.374
1.00
17.64


ATOM
4678
O
WAT
W
25
−11.449
4.860
−24.929
1.00
17.45


ATOM
4679
O
WAT
W
26
−17.555
17.679
−39.815
1.00
23.23


ATOM
4680
O
WAT
W
27
10.075
17.015
−49.295
1.00
24.05


ATOM
4681
O
WAT
W
28
−16.018
−0.740
−24.205
1.00
18.07


ATOM
4682
O
WAT
W
29
9.446
24.991
−37.612
1.00
19.20


ATOM
4683
O
WAT
W
30
−4.165
26.137
−12.642
1.00
18.33


ATOM
4684
O
WAT
W
31
2.771
22.947
−14.916
1.00
25.80


ATOM
4685
O
WAT
W
32
−12.297
21.394
−35.680
1.00
14.89


ATOM
4686
O
WAT
W
33
−24.061
13.570
10.081
1.00
24.96


ATOM
4687
O
WAT
W
34
10.032
29.725
−56.684
1.00
26.97


ATOM
4688
O
WAT
W
35
0.231
4.133
−28.595
1.00
17.67


ATOM
4689
O
WAT
W
36
0.335
2.173
−30.650
1.00
18.32


ATOM
4690
O
WAT
W
37
−10.199
24.315
−42.717
1.00
22.38


ATOM
4691
O
WAT
W
38
−14.151
12.872
−8.204
1.00
16.16


ATOM
4692
O
WAT
W
39
−2.710
9.564
−16.092
1.00
14.10


ATOM
4693
O
WAT
W
40
5.954
7.990
−32.401
1.00
16.59


ATOM
4694
O
WAT
W
41
0.294
5.561
−25.249
1.00
17.87


ATOM
4695
O
WAT
W
42
2.102
15.148
−37.718
1.00
14.64


ATOM
4696
O
WAT
W
43
−19.351
1.384
−26.295
1.00
20.27


ATOM
4697
O
WAT
W
44
−19.623
9.533
−17.751
1.00
14.67


ATOM
4698
O
WAT
W
45
3.117
18.767
−36.336
1.00
12.66


ATOM
4699
O
WAT
W
46
−15.016
16.950
0.662
1.00
20.14


ATOM
4700
O
WAT
W
47
−22.261
4.600
−10.993
1.00
16.38


ATOM
4701
O
WAT
W
48
−12.926
5.474
−22.680
1.00
19.85


ATOM
4702
O
WAT
W
49
5.564
17.071
−37.018
1.00
16.82


ATOM
4703
O
WAT
W
50
−19.848
20.552
−2.718
1.00
19.38


ATOM
4704
O
WAT
W
51
−15.859
17.744
−41.901
1.00
19.69


ATOM
4705
O
WAT
W
52
−16.430
25.522
−1.123
1.00
19.97


ATOM
4706
O
WAT
W
53
−15.978
5.366
−12.193
1.00
26.02


ATOM
4707
O
WAT
W
54
−1.637
9.365
−26.035
1.00
14.42


ATOM
4708
O
WAT
W
55
−10.759
27.212
−30.898
1.00
17.28


ATOM
4709
O
WAT
W
56
−11.509
0.756
−13.101
1.00
20.70


ATOM
4710
O
WAT
W
57
−16.950
15.108
4.727
1.00
23.86


ATOM
4711
O
WAT
W
58
−25.368
26.009
−7.106
1.00
25.08


ATOM
4712
O
WAT
W
59
−16.870
22.937
−3.651
1.00
17.56


ATOM
4713
O
WAT
W
60
−14.388
13.258
−40.897
1.00
27.90


ATOM
4714
O
WAT
W
61
−1.509
−4.779
−6.723
1.00
31.01


ATOM
4715
O
WAT
W
62
−1.973
27.723
−11.521
1.00
21.99


ATOM
4716
O
WAT
W
63
−1.159
−10.623
−29.592
1.00
36.68


ATOM
4717
O
WAT
W
64
−1.943
16.930
−42.957
1.00
21.57


ATOM
4718
O
WAT
W
65
−1.507
25.238
−40.032
1.00
31.36


ATOM
4719
O
WAT
W
66
−4.023
5.499
−31.787
1.00
20.03


ATOM
4720
O
WAT
W
67
−13.383
13.873
−21.065
1.00
12.01


ATOM
4721
O
WAT
W
68
−15.098
10.726
−24.467
1.00
24.34


ATOM
4722
O
WAT
W
69
−2.122
13.975
−13.435
1.00
12.51


ATOM
4723
O
WAT
W
70
−4.807
19.360
−43.270
1.00
20.93


ATOM
4724
O
WAT
W
71
−26.028
26.143
−33.768
1.00
28.78


ATOM
4725
O
WAT
W
72
−19.347
21.638
3.482
1.00
19.92


ATOM
4726
O
WAT
W
73
−27.299
24.219
−6.045
1.00
20.97


ATOM
4727
O
WAT
W
74
−21.114
−0.343
−28.050
1.00
22.38


ATOM
4728
O
WAT
W
75
−5.818
34.483
−11.645
1.00
20.61


ATOM
4729
O
WAT
W
76
6.048
1.098
−23.393
1.00
16.77


ATOM
4730
O
WAT
W
77
−3.946
23.711
−39.552
1.00
25.07


ATOM
4731
O
WAT
W
78
−18.572
21.631
−41.884
1.00
25.98


ATOM
4732
O
WAT
W
79
5.239
26.273
−31.646
1.00
27.95


ATOM
4733
O
WAT
W
80
0.054
15.597
−45.905
1.00
28.45


ATOM
4734
O
WAT
W
81
−3.130
21.534
−5.652
1.00
20.95


ATOM
4735
O
WAT
W
82
−12.534
4.331
−20.095
1.00
17.49


ATOM
4736
O
WAT
W
83
0.785
16.541
−14.558
1.00
14.65


ATOM
4737
O
WAT
W
84
−5.197
12.827
−31.553
1.00
14.10


ATOM
4738
O
WAT
W
85
−16.738
26.994
−34.463
1.00
23.74


ATOM
4739
O
WAT
W
86
3.596
22.076
−36.828
1.00
22.68


ATOM
4740
O
WAT
W
87
5.170
14.460
−40.572
1.00
26.56


ATOM
4741
O
WAT
W
88
−12.322
21.050
0.328
1.00
30.36


ATOM
4742
O
WAT
W
89
7.426
14.327
−48.857
1.00
26.44


ATOM
4743
O
WAT
W
90
−13.702
19.025
1.863
1.00
28.28


ATOM
4744
O
WAT
W
91
8.794
2.010
−23.444
1.00
34.69


ATOM
4745
O
WAT
W
92
−6.185
5.529
−30.210
1.00
17.03


ATOM
4746
O
WAT
W
93
−18.081
20.709
−4.839
1.00
17.61


ATOM
4747
O
WAT
W
94
−15.469
13.082
−22.717
1.00
15.55


ATOM
4748
O
WAT
W
95
13.101
16.811
−29.771
1.00
29.16


ATOM
4749
O
WAT
W
96
−25.944
7.031
−2.628
1.00
27.42


ATOM
4750
O
WAT
W
97
−4.552
34.207
−7.388
1.00
22.19


ATOM
4751
O
WAT
W
98
−2.231
−9.858
−32.291
1.00
26.28


ATOM
4752
O
WAT
W
99
5.314
10.271
−28.762
1.00
29.39


ATOM
4753
O
WAT
W
100
−15.379
27.478
−46.620
1.00
37.77


ATOM
4754
O
WAT
W
101
26.815
24.874
−36.295
1.00
31.11


ATOM
4755
O
WAT
W
102
−18.489
−0.112
−24.256
1.00
23.63


ATOM
4756
O
WAT
W
103
−23.763
26.890
−9.454
1.00
20.50


ATOM
4757
O
WAT
W
104
−10.933
23.904
−50.315
1.00
25.63


ATOM
4758
O
WAT
W
105
5.864
12.071
−41.668
1.00
29.27


ATOM
4759
O
WAT
W
106
2.526
9.409
−13.116
1.00
20.75


ATOM
4760
O
WAT
W
107
−11.557
−6.653
−10.981
1.00
30.68


ATOM
4761
O
WAT
W
108
−14.882
7.238
−22.254
1.00
24.81


ATOM
4762
O
WAT
W
109
−5.331
−13.390
−25.293
1.00
35.63


ATOM
4763
O
WAT
W
110
−8.068
24.248
−40.534
1.00
39.14


ATOM
4764
O
WAT
W
111
−0.779
14.419
−43.060
1.00
24.01


ATOM
4765
O
WAT
W
112
−22.279
12.054
−26.750
1.00
32.13


ATOM
4766
O
WAT
W
113
−26.829
1.352
−33.787
1.00
26.97


ATOM
4767
O
WAT
W
114
−14.120
14.116
3.214
1.00
36.89


ATOM
4768
O
WAT
W
115
0.582
−9.914
−21.103
1.00
23.30


ATOM
4769
O
WAT
W
116
−24.305
22.723
6.995
1.00
21.67


ATOM
4770
O
WAT
W
117
−28.275
12.468
−15.419
1.00
21.87


ATOM
4771
O
WAT
W
118
3.699
27.669
−20.781
1.00
34.08


ATOM
4772
O
WAT
W
119
−30.428
26.452
3.757
1.00
24.51


ATOM
4773
O
WAT
W
120
19.168
26.858
−59.022
1.00
33.45


ATOM
4774
O
WAT
W
121
−8.803
21.729
−1.693
1.00
18.30


ATOM
4775
O
WAT
W
122
2.863
1.621
−31.755
1.00
19.96


ATOM
4776
O
WAT
W
123
−2.357
28.930
−56.725
1.00
35.51


ATOM
4777
O
WAT
W
124
−16.780
5.504
−21.523
1.00
31.34


ATOM
4778
O
WAT
W
125
6.216
18.141
−30.592
1.00
20.87


ATOM
4779
O
WAT
W
126
11.789
32.722
−38.773
1.00
40.41


ATOM
4780
O
WAT
W
127
−5.001
7.195
−45.656
1.00
35.03


ATOM
4781
O
WAT
W
128
−18.743
1.608
−1.861
1.00
32.49


ATOM
4782
O
WAT
W
129
−25.089
−1.945
−20.935
1.00
35.17


ATOM
4783
O
WAT
W
130
−7.097
−2.177
−28.928
1.00
30.93


ATOM
4784
O
WAT
W
131
−12.591
2.907
−11.929
1.00
18.60


ATOM
4785
O
WAT
W
132
−17.913
−2.374
−39.429
1.00
29.36


ATOM
4786
O
WAT
W
133
−6.507
−7.038
−37.710
1.00
37.27


ATOM
4787
O
WAT
W
134
−0.628
7.596
−18.660
1.00
20.01


ATOM
4788
O
WAT
W
135
−11.683
28.527
−37.016
1.00
36.45


ATOM
4789
O
WAT
W
136
−3.169
33.267
−18.049
1.00
24.89


ATOM
4790
O
WAT
W
137
−16.742
8.938
−23.161
1.00
26.79


ATOM
4791
O
WAT
W
138
−28.456
17.726
−22.449
1.00
32.61


ATOM
4792
O
WAT
W
139
25.559
27.237
−45.392
1.00
43.21


ATOM
4793
O
WAT
W
140
−26.925
5.789
−41.722
1.00
26.97


ATOM
4794
O
WAT
W
141
−16.907
20.013
−43.283
1.00
29.68


ATOM
4795
O
WAT
W
142
−20.029
5.119
−1.799
1.00
24.76


ATOM
4796
O
WAT
W
143
8.706
1.050
−13.115
1.00
32.81


ATOM
4797
O
WAT
W
144
−4.353
22.506
−1.252
1.00
24.86


ATOM
4798
O
WAT
W
145
−29.660
14.750
−15.295
1.00
32.62


ATOM
4799
O
WAT
W
146
2.173
2.240
−12.124
1.00
26.38


ATOM
4800
O
WAT
W
147
4.174
−4.659
−14.794
1.00
25.20


ATOM
4801
O
WAT
W
148
−10.913
29.083
−33.130
1.00
27.78


ATOM
4802
O
WAT
W
149
−21.448
30.157
−10.670
1.00
25.07


ATOM
4803
O
WAT
W
150
−23.296
18.641
−36.646
1.00
27.11


ATOM
4804
O
WAT
W
151
−19.426
8.262
−24.240
1.00
25.11


ATOM
4805
O
WAT
W
152
4.729
−0.512
−31.679
1.00
23.50


ATOM
4806
O
WAT
W
153
9.247
19.703
−33.306
1.00
23.44


ATOM
4807
O
WAT
W
154
6.024
15.401
−22.768
1.00
27.11


ATOM
4808
O
WAT
W
155
−16.077
30.180
−4.530
1.00
23.52


ATOM
4809
O
WAT
W
156
−0.038
14.751
−8.812
1.00
25.64


ATOM
4810
O
WAT
W
157
2.962
18.631
−29.190
1.00
18.13


ATOM
4811
O
WAT
W
158
8.793
12.371
−36.745
1.00
23.77


ATOM
4812
O
WAT
W
159
−22.406
9.468
−0.415
1.00
21.66


ATOM
4813
O
WAT
W
160
−10.961
33.685
−7.076
1.00
25.30


ATOM
4814
O
WAT
W
161
−8.504
27.891
−3.964
1.00
33.88


ATOM
4815
O
WAT
W
162
6.836
20.663
−32.439
1.00
24.97


ATOM
4816
O
WAT
W
163
4.292
23.232
−29.206
1.00
32.74


ATOM
4817
O
WAT
W
164
2.350
3.656
−15.645
1.00
23.29


ATOM
4818
O
WAT
W
165
−17.377
10.190
−20.605
1.00
25.21


ATOM
4819
O
WAT
W
166
−23.426
24.714
4.551
1.00
26.12


ATOM
4820
O
WAT
W
167
0.338
1.730
−14.995
1.00
31.29


ATOM
4821
O
WAT
W
168
−3.303
17.836
−46.350
1.00
28.34


ATOM
4822
O
WAT
W
169
1.465
6.514
−14.840
1.00
22.81


ATOM
4823
O
WAT
W
170
2.409
11.466
−4.481
1.00
29.82


ATOM
4824
O
WAT
W
171
0.998
19.313
−20.348
1.00
31.57


ATOM
4825
O
WAT
W
172
7.556
−3.076
−34.213
1.00
31.62


ATOM
4826
O
WAT
W
173
−25.163
1.132
−18.852
1.00
33.45


ATOM
4827
O
WAT
W
174
−25.606
17.471
−26.509
1.00
27.89


ATOM
4828
O
WAT
W
175
5.952
32.621
−65.955
1.00
42.20


ATOM
4829
O
WAT
W
176
−27.397
26.421
−12.489
1.00
29.06


ATOM
4830
O
WAT
W
177
−17.506
35.918
−29.284
1.00
36.40


ATOM
4831
O
WAT
W
178
−18.298
7.055
−19.628
1.00
30.04


ATOM
4832
O
WAT
W
179
−24.383
14.811
−26.605
1.00
29.51


ATOM
4833
O
WAT
W
180
−1.204
27.462
−35.328
1.00
29.93


ATOM
4834
O
WAT
W
181
−14.112
33.822
−23.916
1.00
34.66


ATOM
4835
O
WAT
W
182
2.887
26.714
−9.619
1.00
34.18


ATOM
4836
O
WAT
W
183
−16.062
4.698
1.046
1.00
32.44


ATOM
4837
O
WAT
W
184
−13.340
36.111
−4.359
1.00
39.14


ATOM
4838
O
WAT
W
185
9.661
34.457
−47.977
1.00
37.66


ATOM
4839
O
WAT
W
186
−8.465
24.284
−1.237
1.00
33.71


ATOM
4840
O
WAT
W
187
16.971
15.520
−43.951
1.00
42.49


ATOM
4841
O
WAT
W
188
−12.038
−14.614
−20.299
1.00
34.37


ATOM
4842
O
WAT
W
189
−5.887
22.387
−40.784
1.00
33.70


ATOM
4843
O
WAT
W
190
−3.962
−18.100
−17.720
1.00
31.33


ATOM
4844
O
WAT
W
191
−30.888
11.643
−15.288
1.00
36.84


ATOM
4845
O
WAT
W
192
11.576
13.142
−37.752
1.00
32.89


ATOM
4846
O
WAT
W
193
−7.856
3.348
−41.927
1.00
34.02


ATOM
4847
O
WAT
W
194
−20.849
7.518
7.652
1.00
32.37


ATOM
4848
O
WAT
W
195
16.954
13.938
−58.514
1.00
42.65


ATOM
4849
O
WAT
W
196
−31.884
7.593
−13.893
1.00
37.54


ATOM
4850
O
WAT
W
197
4.560
−14.190
−17.137
1.00
36.09


ATOM
4851
O
WAT
W
198
1.116
27.617
−39.051
1.00
37.08


ATOM
4852
O
WAT
W
199
−1.019
−12.134
−21.800
1.00
36.12


ATOM
4853
O
WAT
W
200
8.350
0.111
−21.198
1.00
36.56


ATOM
4854
O
WAT
W
201
−2.691
31.235
−26.910
1.00
32.08


ATOM
4855
O
WAT
W
202
13.222
30.530
−38.626
1.00
36.46


ATOM
4856
O
WAT
W
203
−11.218
19.535
−54.549
1.00
35.12


ATOM
4857
O
WAT
W
204
−5.623
10.865
−46.910
1.00
35.48


ATOM
4858
O
WAT
W
205
−18.073
1.743
−43.946
1.00
40.62


ATOM
4859
O
WAT
W
206
−32.195
23.231
2.102
1.00
34.73


ATOM
4860
O
WAT
W
207
−24.204
8.994
−2.941
1.00
30.29


ATOM
4861
O
WAT
W
208
−4.771
18.292
−48.610
1.00
31.87


ATOM
4862
O
WAT
W
209
−17.156
23.843
−40.674
1.00
35.73


ATOM
4863
O
WAT
W
210
8.319
13.422
−13.297
1.00
37.43


ATOM
4864
O
WAT
W
211
−25.962
8.559
−33.791
1.00
33.46


ATOM
4865
O
WAT
W
212
−36.129
8.276
3.147
1.00
40.24


ATOM
4866
O
WAT
W
213
20.833
21.074
−56.185
1.00
39.11


ATOM
4867
O
WAT
W
214
−17.726
14.087
8.330
1.00
39.10


ATOM
4868
O
WAT
W
215
8.944
8.011
−10.493
1.00
41.24


ATOM
4869
O
WAT
W
216
−16.566
35.858
−11.282
1.00
38.90


ATOM
4870
O
WAT
W
217
−20.560
11.198
−43.128
1.00
34.83


ATOM
4871
O
WAT
W
218
3.261
−0.833
−39.177
1.00
32.67


ATOM
4872
O
WAT
W
219
−22.370
−13.152
−34.412
1.00
59.42


ATOM
4873
O
WAT
W
220
−24.775
6.925
5.968
1.00
34.28


ATOM
4874
O
WAT
W
221
−20.357
21.098
−45.702
1.00
36.83


ATOM
4875
O
WAT
W
222
2.502
28.932
−40.686
1.00
36.85


ATOM
4876
O
WAT
W
223
−17.630
−5.533
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1.00
35.08


ATOM
4877
O
WAT
W
224
−19.358
−1.912
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1.00
35.83


ATOM
4878
O
WAT
W
225
−14.632
25.995
−42.094
1.00
41.12


ATOM
4879
O
WAT
W
226
−28.967
5.606
−22.103
1.00
45.63


ATOM
4880
O
WAT
W
227
−4.326
9.934
5.097
1.00
44.46


ATOM
4881
O
WAT
W
228
3.983
22.711
−17.336
1.00
46.21


ATOM
4882
O
WAT
W
229
−17.238
16.931
6.949
1.00
42.44


ATOM
4883
O
WAT
W
230
−25.871
18.809
10.364
1.00
36.15


ATOM
4884
O
WAT
W
231
−23.524
31.294
−19.082
1.00
35.99


ATOM
4885
O
WAT
W
232
−5.261
−9.321
−38.277
1.00
43.42


ATOM
4886
O
WAT
W
233
−22.757
28.188
−29.173
1.00
39.56


ATOM
4887
O
WAT
W
234
−25.699
26.238
−0.299
1.00
40.26


ATOM
4888
O
WAT
W
235
−21.884
−4.007
−22.294
1.00
38.01


ATOM
4889
O
WAT
W
236
−6.696
−17.346
−27.753
1.00
39.83


ATOM
4890
O
WAT
W
237
−18.052
5.515
3.888
1.00
37.82


ATOM
4891
O
WAT
W
238
−6.073
35.708
−9.057
1.00
36.25


ATOM
4892
O
WAT
W
239
−8.876
2.984
−44.368
1.00
45.85


ATOM
4893
O
WAT
W
240
9.232
31.613
−65.496
1.00
45.82


ATOM
4894
O
WAT
W
241
−28.246
26.806
−0.118
1.00
36.45


ATOM
4895
O
WAT
W
242
−27.793
14.675
−45.312
1.00
52.10


ATOM
4896
O
WAT
W
243
7.463
14.079
−55.045
1.00
36.97


ATOM
4897
O
WAT
W
244
−28.572
4.769
−1.130
1.00
36.20


ATOM
4898
O
WAT
W
245
8.221
12.936
−50.870
1.00
38.81


ATOM
4899
O
WAT
W
246
−23.302
−2.082
−27.191
1.00
32.98


ATOM
4900
O
WAT
W
247
−13.035
8.248
−46.620
1.00
51.73


ATOM
4901
O
WAT
W
248
−11.869
31.852
−50.157
1.00
56.13


ATOM
4902
O
WAT
W
249
0.898
13.920
−6.521
1.00
27.38


ATOM
4903
O
WAT
W
250
20.427
30.852
−45.446
1.00
35.54


ATOM
4904
O
WAT
W
251
−1.397
12.400
−44.617
1.00
39.33


ATOM
4905
O
WAT
W
252
−27.354
24.696
−3.162
1.00
35.38


ATOM
4906
O
WAT
W
253
17.587
20.557
−31.069
1.00
41.51


ATOM
4907
O
WAT
W
254
−7.936
35.055
−7.354
1.00
39.02


ATOM
4908
O
WAT
W
255
−22.469
7.215
−2.044
1.00
38.25


ATOM
4909
O
WAT
W
256
2.038
15.474
−52.963
1.00
50.04


ATOM
4910
O
WAT
W
257
10.889
10.184
−21.700
1.00
44.84


ATOM
4911
O
WAT
W
258
−11.714
10.583
4.136
1.00
42.70


ATOM
4912
O
WAT
W
259
−14.719
6.574
2.959
1.00
43.18


ATOM
4913
O
WAT
W
260
−16.694
25.390
−37.688
1.00
36.77


ATOM
4914
O
WAT
W
261
−9.212
13.388
−48.363
1.00
38.05


ATOM
4915
O
WAT
W
264
−0.611
−1.965
−3.253
1.00
37.95


ATOM
4916
O
WAT
W
265
−16.380
30.998
−14.262
1.00
32.44


ATOM
4917
O
WAT
W
266
9.420
16.012
−61.368
1.00
35.22


ATOM
4918
O
WAT
W
267
−4.976
−15.180
−21.223
1.00
45.50


ATOM
4919
O
WAT
W
268
−16.631
33.287
−14.201
1.00
34.50


ATOM
4920
O
WAT
W
269
−16.883
34.052
−32.249
1.00
36.88


ATOM
4921
O
WAT
W
270
−8.293
−16.006
−14.535
1.00
34.80


ATOM
4922
O
WAT
W
273
0.240
4.589
−13.868
1.00
32.34


ATOM
4923
O
WAT
W
275
3.657
14.447
−55.516
1.00
43.54


ATOM
4924
O
WAT
W
276
−17.602
20.784
−51.471
1.00
38.88


ATOM
4925
O
WAT
W
277
−10.479
31.683
−30.513
1.00
40.35


ATOM
4926
O
WAT
W
278
−10.974
4.308
−5.745
1.00
40.30


ATOM
4927
O
WAT
W
280
−4.336
36.908
−17.666
1.00
34.01


ATOM
4928
O
WAT
W
281
6.720
33.970
−53.572
1.00
38.81


ATOM
4929
O
WAT
W
282
−30.457
23.527
−0.621
1.00
34.61


ATOM
4930
O
WAT
W
283
16.969
17.394
−30.816
1.00
55.74


ATOM
4931
O
WAT
W
284
−24.391
5.834
−24.909
1.00
37.92


ATOM
4932
O
WAT
W
285
4.567
9.814
−4.438
1.00
44.83


ATOM
4933
O
WAT
W
286
−24.370
−7.328
−27.875
1.00
56.18


ATOM
4934
O
WAT
W
287
−21.605
11.887
9.715
1.00
43.15


ATOM
4935
O
WAT
W
288
8.603
0.412
−37.887
1.00
40.47


ATOM
4936
O
WAT
W
290
−20.056
21.495
6.073
1.00
41.68


ATOM
4937
O
WAT
W
291
−3.221
28.158
−33.448
1.00
44.24


ATOM
4938
O
WAT
W
292
9.171
9.103
−38.735
1.00
34.30


ATOM
4939
O
WAT
W
293
2.894
22.763
−25.829
1.00
38.57


ATOM
4940
O
WAT
W
294
−29.901
19.604
−14.929
1.00
37.66


ATOM
4941
O
WAT
W
296
−4.579
30.229
−29.110
1.00
40.57


ATOM
4942
O
WAT
W
297
−23.821
11.441
−33.187
1.00
42.81


ATOM
4943
O
WAT
W
298
−26.753
−3.087
−31.243
1.00
39.08


ATOM
4944
O
WAT
W
300
−10.820
35.024
−53.050
1.00
55.39


ATOM
4945
O
WAT
W
302
−1.992
7.169
−31.692
1.00
39.12


ATOM
4946
O
WAT
W
303
−15.282
−19.000
−23.770
1.00
34.62


ATOM
4947
O
WAT
W
304
12.106
10.568
−25.112
1.00
39.04


ATOM
4948
O
WAT
W
305
2.585
2.766
1.880
1.00
53.88


ATOM
4949
O
WAT
W
306
3.680
21.122
−19.818
1.00
46.35


ATOM
4950
O
WAT
W
307
22.759
24.721
−48.099
1.00
40.35


ATOM
4951
O
WAT
W
309
−17.062
−6.726
−19.202
1.00
41.54


ATOM
4952
O
WAT
W
311
12.594
1.109
−31.461
1.00
47.85


ATOM
4953
O
WAT
W
312
23.347
25.060
−50.638
1.00
49.64


ATOM
4954
O
WAT
W
314
−18.291
4.422
−19.151
1.00
39.17


ATOM
4955
O
WAT
W
315
−11.815
−7.807
−8.676
1.00
37.74


ATOM
4956
O
WAT
W
316
−25.147
1.885
−4.649
1.00
44.72


ATOM
4957
O
WAT
W
317
−36.473
13.592
5.315
1.00
44.38


ATOM
4958
O
WAT
W
318
−17.587
20.023
−46.231
1.00
48.30


ATOM
4959
O
WAT
W
319
−16.081
29.024
−54.668
1.00
39.40


ATOM
4960
O
WAT
W
320
−14.210
32.143
−5.494
1.00
42.73


ATOM
4961
O
WAT
W
321
−15.274
28.830
−38.916
1.00
46.47


ATOM
4962
O
WAT
W
322
−32.792
22.221
−3.433
1.00
41.52


ATOM
4963
O
WAT
W
323
−32.475
16.905
−12.401
1.00
46.29


ATOM
4964
O
WAT
W
325
15.341
22.212
−60.490
1.00
34.67


ATOM
4965
O
WAT
W
326
−12.668
8.518
−41.723
1.00
36.26


ATOM
4966
O
WAT
W
327
4.709
20.490
−10.568
1.00
38.04


ATOM
4967
O
WAT
W
328
13.937
10.625
−29.312
1.00
38.32


ATOM
4968
O
WAT
W
329
−21.964
9.615
−24.896
1.00
40.43


ATOM
4969
O
WAT
W
330
19.325
25.925
−40.199
1.00
51.36


ATOM
4970
O
WAT
W
331
−19.010
8.073
−45.255
1.00
46.06


ATOM
4971
O
WAT
W
332
−25.024
−2.892
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1.00
41.74


ATOM
4972
O
WAT
W
333
−16.593
−7.067
−23.297
1.00
38.54


ATOM
4973
O
WAT
W
334
−17.517
24.078
2.157
1.00
45.82


ATOM
4974
O
WAT
W
335
−19.123
31.941
0.010
1.00
38.91


ATOM
4975
O
WAT
W
337
10.677
21.901
−62.740
1.00
44.31


ATOM
4976
O
WAT
W
338
4.510
15.230
−51.810
1.00
42.12


ATOM
4977
O
WAT
W
339
13.979
14.161
−43.380
1.00
46.42


ATOM
4978
O
WAT
W
341
5.979
−11.625
−28.739
1.00
43.09


ATOM
4979
O
WAT
W
342
−19.453
13.347
10.394
1.00
42.44


ATOM
4980
O
WAT
W
343
7.085
23.050
−30.796
1.00
34.94


ATOM
4981
O
WAT
W
345
6.471
24.087
−63.943
1.00
45.16


ATOM
4982
O
WAT
W
347
3.734
22.842
−12.031
1.00
43.85


ATOM
4983
O
WAT
W
348
−17.739
7.564
5.723
1.00
43.77


ATOM
4984
O
WAT
W
351
−22.014
31.372
−24.708
1.00
42.69


ATOM
4985
O
WAT
W
352
25.016
25.103
−46.967
1.00
40.51


ATOM
4986
O
WAT
W
353
7.969
32.464
−67.637
1.00
57.78


ATOM
4987
O
WAT
W
354
−27.444
5.101
5.861
1.00
48.31


ATOM
4988
O
WAT
W
356
8.012
11.087
−40.867
1.00
47.51


ATOM
4989
O
WAT
W
357
4.974
29.116
−17.433
1.00
43.72


ATOM
4990
O
WAT
W
358
−0.457
9.488
−45.288
1.00
45.63


ATOM
4991
O
WAT
W
360
−3.090
36.536
−12.138
1.00
46.29


ATOM
4992
O
WAT
W
361
20.072
19.772
−36.896
1.00
38.73


ATOM
4993
O
WAT
W
363
−26.217
15.345
−28.735
1.00
49.07


ATOM
4994
O
WAT
W
365
−25.308
0.100
−48.602
1.00
60.01


ATOM
4995
O
WAT
W
367
19.369
29.586
−58.438
1.00
48.19


ATOM
4996
O
WAT
W
369
12.808
11.144
−54.427
1.00
48.48


ATOM
4997
O
WAT
W
370
9.410
2.674
−16.115
1.00
44.59


ATOM
4998
O
WAT
W
372
−10.249
38.564
−13.215
1.00
48.60


ATOM
4999
O
WAT
W
373
−24.151
16.211
10.617
1.00
42.16


ATOM
5000
O
WAT
W
375
−6.459
31.697
−48.106
1.00
46.03


ATOM
5001
O
WAT
W
376
−11.605
27.116
−1.562
1.00
44.58


ATOM
5002
O
WAT
W
377
−4.703
24.150
−62.673
1.00
48.70


ATOM
5003
O
WAT
W
379
6.889
0.036
−7.530
1.00
45.35


ATOM
5004
O
WAT
W
381
−13.601
32.742
−32.002
1.00
51.37


ATOM
5005
O
WAT
W
383
−28.077
5.243
−4.688
1.00
37.46


END

## Claims

1. A polynucleotide encoding a glucoamylase variant comprising amino acid substitutions at the positions corresponding to positions 417, 430, and 563 of SEQ ID NO: 2, or equivalent positions in a parent glucoamylase having at least 90% sequence identity to SEQ ID NO: 1 or 2, and
wherein the glucoamylase variant has at least 90% sequence identity with SEQ ID NO: 1 or 2 and glucoamylase activity.

2. A vector comprising the polynucleotide of claim 1.

3. A host cell comprising the vector of claim 2.

4. A method of producing a glucoamylase variant in a host cell comprising culturing the host cell of claim 3 under conditions suitable for the expression and production of the glucamylase variant and producing the glucoamylase variant.

5. The method according to claim 4 further comprising recovering the glucoamylase variant from the culture.