Cytokinins

116801, 116802

Product Chemistry—Registration Review

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY

MEMORANDUM

SUBJECT: Product Chemistry Scoping Document for the Registration Review o

Name. 

Registration Review Case #:

PC Code:

CAS Numbers:

Chemical Class:

FROM: Sadaf Shaukat, Biologist

Biochemical Pesticides Branch

Biopesticides & Pollution Prevention Division (7511P)

TO: Cheryl Greene, Regulatory Action Leader

Biochemical Pesticides Branch

Biopesticides & Pollution Prevention Division (7511P) 

ACTION REQUESTED

A preliminary assessment of available information on product chemistry data associated with 

the biopesticide cytokinins in support of the development of a Registration Review Work Plan.

Case Number: 

Registration Review

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY

WASHINGTON, D.C. 20460

8/15/2012

OFFICE OF CHEMICAL SAFETY AND POLLUTION PREVENTION

Product Chemistry Scoping Document for the Registration Review o

Registration Review Case #: 4107

116801, 116802

525-79-1

Biochemical

Biologist /s/ 8/15/12

Biochemical Pesticides Branch

Biopesticides & Pollution Prevention Division (7511P)

, Regulatory Action Leader

Biochemical Pesticides Branch

Pollution Prevention Division (7511P) 

A preliminary assessment of available information on product chemistry data associated with 

in support of the development of a Registration Review Work Plan.

Case Number: 4107

OFFICE OF CHEMICAL SAFETY AND POLLUTION PREVENTION

Product Chemistry Scoping Document for the Registration Review of Chemical 

A preliminary assessment of available information on product chemistry data associated with 

in support of the development of a Registration Review Work Plan.
Cytokinins Case Number: 4107

116801, 116802

Introduction

The EPA has made the determination that there is adequate information available on 

cytokinins, including cytokinin, kinetin, and N6-benzyladenine, and does not foresee the 

need for additional biochemical pesticide product chemistry data for products that 

currently contain cytokinins as the technical grade active ingredient. The three 

substances are plant growth regulators, which enhance the growth and development of 

plants. Cytokinin products have been registered with the EPA since 1978. Currently there 

are sixty-eight active registrations of products containing cytokinins. The Agency

exempts cytokinins from the requirement of a tolerance when used as a plant regulator on 

plants, seeds, or cuttings and on all food commodities after harvest (see 40 CFR 

180.1157). In addition, the Agency exempts N6-benzyladenine from the requirement of a 

tolerance when used in or on apple and pear at a rate of ≤182 grams of active ingredient 

per acre per season, and in or on pistachio when applied at a rate of ≤60 grams of active 

ingredient per acre per season (see 40 CFR 180.1150).

Concerning the product chemistry of cytokinins, all biochemical pesticide data 

requirements for the technical grade active ingredient have been satisfied and are 

summarized in Table 2. While no further product chemistry data are necessary for the 

products currently registered, future registrations must adequately address all of the 

biochemical pesticides product chemistry data requirements.
Cytokinins Case Number: 4107

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Table 1. Product Identity and Composition for Cytokinin/Kinetin

Common Name Cytokinin/Kinetin

Chemical Name, IUPAC N-(2-Furanylmethyl)-9H-purin-6-amine

Molecular Weight 215.21 g/mol

PC Code 116801/116802

CAS Registry Number 525-79-1

Empirical Formula C10H9N5O

Registration Review Case Number 4107

Chemical Structure
Cytokinins Case Number: 4107

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Product Chemistry Summary

The Agency has conducted a review of the available product chemistry data and information. 

These data are summarized below.

Table 2. Biochemical Pesticides Product Chemistry Data Requirements for Chemical Name

Guideline No. Physical and Chemical 

Properties

Status

1

Value MRID/

Source

830.1100 Product Identity and 

Composition

A Refer to Table 1. N/A

830.1200 Description of starting 

materials, production and 

formulation process

A CBI 48541201

830.1400 Discussion of formation of 

impurities

A CBI 48541201

830.1700 Preliminary analysis A CBI 48541202

830.6302 Color A White 44911003

830.6303 Physical state A Crystals 44911003

830.6304 Odor A Odorless 44911003

830.6313 Stability to normal and 

elevated temperatures, 

metals and metal ions

N/A

830.7000 pH A 7.4 (25oC) 44911003

830.7050 UV/Visible light absorption N/A

830.7200 Melting point/melting range A 265-266 oC 44911003

830.7220 Boiling point/boiling range A 524.4 0C (at 760 mmHg) LookChem

830.7300 Density A 1.4374 g/cm3

44911003

830.7520 Particle size, fiber length, 

and diameter distribution

N/A

830.7550

830.7560

830.7570

Partition coefficient (nOctanol/Water)N/A

830.7840 Water solubility A 51.0 mg/L (25oC) 44911003

830.7950 Vapor pressure A Negligible FisherScien

-tific

1. A=Acceptable. N/A=Not Applicable. N/D=Not Determined
Cytokinins Case Number: 4107

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References

Fisher Scientific, Online. 8/15/2012. 

http://www.fishersci.com/ecomm/servlet/msdsproxy?productName=BP9421&productDescriptio

n=KINETIN+1GM&catNo=BP942-1&vendorId=VN00033897&storeId=10652

LookChem, Online. 08/15/2012. http://www.lookchem.com/cas-525/525-79-1.html

Merck Index, Online. 08/14/2012.

Shi, Xiaoluo and Qin, Guohong. Color, Physical State, Odor, Melting Point, Density, Solubility

and pH of Kinetin Technical. Private Study by LT BioSyn, Inc. Submitted to EPA. MRID 

44911003.