14 JUN 1983

One Source.

N

One Search. One Solutio

U.S. Department of Commerce

National Technical Information s~

. -

OTS0545948

111111111111111111111111111111111111111111111111111111111111

INITIAL SUBMISSION: ONE-MONTH INHALATION

TOXICITY OF METHYL IODIDE TO MALE AND

FEMALE SPRAGUE-DAWLEY RATS WITH COVER

LETTER DATED 080792
Microfiche No.

.'

8ECP

8EHQ-Q892-9501

OTS0545948

08127/92

88-920007803

MONSANTO CO

06114/83

METIIYL IODIDE

CODING FORMS FOR SRC INDEXING

Chemical Category

INITIAL SUBMiSSION: ONE-MONTH INHALATION TOXICITY OF METHYL

IODIDE TO MALE AND FEMALE SPRAGUE-DAWLEY RATS WTI1I COVER

DATED 080792
COpy I 1 PUBLIC FILE

COpy # 2 JIM DARR/Vivian

COMPANY SANrnzeo . TDAS (BLAn:) ORIGINAL PUBLIC FILE

CBIC (Vera)

..

ORIGINAL

(COMPLIANCE AUDIT PROGRAM)

sce)

COpy I 1

COpy I 2

TSCA CONfIDENTIAL

·BUSINESS INFORMAnON

NOTE: Peter provi.des data entry in OIl'S for the See) CAP Documents.
The information included herein is characterized as follows:

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88928887883

IHIT 88/27--92

1I1111111111

Illllllll

6tJh-o~9}-- 9su/

DCcntains NO CBI"

,

Sine:erely,

J.

~~R. Condray

Director, Regulatory Management

(314) 694-8883

Information/Study Type - II.B,2,b/Subcbronic: Toxicity Study

Information/Study Identification - ONE-MONTH INHAlATION TOXICITY OF METHYL

IODIDE TO MALE AND FEMALE SPRAGUE-DAWLEY RATS

ML-81-Q84

Pr~ous TSCA. 8(e) or PMN submissions, if any, for the referencc c:bemical can be fOUlld in

AppcndixA

Chemical Identity - IOoo METHANE; methyl iodide; CP 10340

Chemical CAS No. - 000074884

Identification of Reportable Endpoint; SIGNS OF NEUROTOXICITY

Registered Mail

Return Receipt Requested

Attention: Section 8(e) a>ordinator (CAP Agreement)

Monsanto

Llonsatto Company

8CIO N. Lindbergh Ilo.JIevard

Sl. Louis, Mi5:ID.lri 63167

Phone: (314) 694-1000

August 7,1992

Document ProccssiDg Center (TS-790)

Office of Toxic: Substances

Euvironmenta1 Protection Agency

401 M Street. SW

Washington, DC 20460

It should be noted that this summary is not all inclusive. Therefore, it may not higbligbt aD

adverse effects that EPA may judge to meet TSCA 8(e) reportability. This submission/report does

not contain confidential business information.

This submission is pursuant to the TSCA Secrion 8(e) Compliance Audit Program and

CAP Agreement #8ECAP-00'36.

Triplicate Copies Enclosed
JUN 141983

C0TfcQra tjfEPS IOMEH fF:HT.

COJDIV EPTJLOCAnONI

___-=~F~IN~AL~..""",..._---REPORT

rTYPe OF REPORTl

,

ABSTRACT: Four groups of 10 male and lO female SpragueDawley rats per group were each exposed to 0,

0.l42, O.42l or O.BlO mg methyl iodide per liter

of air in lO m3 inhalation chambers six hours per

workday over an approximate four-week period (21

exposure days). Rypoactivity, hyperactivity,

irritation of the eyes and/or nose, breathing

difficulties, and tremors, twitches and/or convulsions were observed during exposure. Irritation

of the eyes, hypoactivity, nasal discharge,

respiratory difficulties, abnormal appearance of

the skin and fur and a singular occurxence of trem::lrs

were observed in the non-exposure periods. These

gross signs of toxicity were concentration-related

in that they were observed primarily in the high

exposure group and to a lesser extent in the midexposure group. Compound and concentration-related

decreases in mean body weight were noted in the midand high exposure groups. Five test material-related

deaths occurred in the high exposure group: 1

spontaneous death (1 female) and 4 sacr:i:ficed in

extremis (2 males, 2 females). No exposure-reIi'ted

C.CONTINtI'ED ON m:vERSE SIDE)

AUTHORS: Thomas L. Blank

TITLE: One-Month :I.nhaJ.ation Toxicity of Methyl Iodide

to Male and Female Sprague-Dawley Rats

REPORT NO.: MSL-3012

JOBIPROJECT NO.: 810006/MI.-8l-084

DATE: June 7, 1983

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EBL 810006

PAGE 1

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June 7, 1983

1f\ \\1J. e~A 2'-\ M_ ,qK~ M~q. RoTc5'm\\-G""'r-o-u-p-""'Le-a-d~e-r-----=-;L..:..~-D~!...a t~e'

Inhalation Toxicology

MONSANTO COMPANY

ENVIRONMENTAL BEALTB LABORATORY

645 s. NEWSTEAD

ST. LOUIS, ~ISSOORI 63110

~~d~ Thomas L. B~nk, Study Director

Study Number: 810006

DMEB Project Number: ML-8l-084

One-Month Inhalation Toxicity of Methyl Iodide to

Male and Female sprague-Dawley Rats

Report Issued:

Author and Study Director: Thomas L. Blank

Submitted to: ~onsanto Industrial Chemicals (Sponsor)

Through: R. s. Nair, staff Toxicologist
SOMMARY

Four groups of 10 male and 10 female Sprague-Dawley rats per

group were each exposed to 0, 0.142, 0.421 or 0.810 mg methyl

iodide per liter of air in 10 m3 inhalation chambers six

hours per workday over an approximate four-week period (21

exposure days). Hypoactivity, hyperactivity, irritation of

the eyes and/or nose, breathing difficulties, and tremors,

twitches and/or convulsions were observed during exposure.

Irritation of the eyes, hypoactivity, nasal discharge,

respiratory difficulties, abnormal appearance of the skin and

fur and a singular occurrence of tremors were observed in the

non-exposure periods. These gross signs of toxicity were

concentration-related in that they were observed primarily in

the high exposure group and to a lesser extent in the

mid-exposure group. Compound and concentration-related

decreases ir. mean body weight were noted in the mid- and high

exposure groups. Five test material-related deaths occurred

in the high exposure group: 1 spontaneous death (1 female) and

4 sacrificed in extremis (2 males, 2 females). No

exposure-related gross pathological lesions were observed in

either the test material-related deaths or terminal sacrifice

animals. A -no-effect- exposure level for male and female

rats receiving 21 six-hour exposures of methyl iodide over an

approximate one-month period was 0.142 mg/l of air.

'.

EHL 810006

PAGE 2

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TABLE OF CONTENTS

TITLE PAGE

SUMMARY

TABLE OF CONTENTS

INTRODUCTION

MATERIALS AND METHODS

·Test Material

.Test System: Animals and Husbandry

.Exposure System

.Procedures for Monitoring Methyl Iodide

in the Inhalation Chamber

·Animal Observations and Body Weights

-Gross Pathology -Statistical Procedures

-Comments on Study Conduct Versus Protocol

RESULTS

-Chamber Atmosphere Analysis and

Environmental Data

-Animal Observations and Body Weights

-Gross Pathology

DISCUSSION AND CONCLUSIONS

REFERENCES

SUPPLEMENTAL STODY INFORMATION

QUALITY ASSURANCE STATEMENT

Figure 1 - Chronic Inhalation Chamber

Figure 2 - Control Panel and Metering System

Figure 3 - Control Panel and Metering System

for sODic Jet

Em. 810006

PAGE 3

Report Page

1

2

3-5

6

6-9

6

6-7

7

7-8

8

8

8

9

9-10

9

10

10

11

12

13

14

15

16

17
Appendix II - In-Life Animal Observations

39-48

35-36

24-27

37-36

32-67

33-34

23

31

30

23-31

28-29

18-31

19-22

Report Page

Mean Daily Nominal Methyl Iodide

Chamber Concentrations

EHL 810006

PAGE 4

Occurrence of Specific Gross signs of Toxicity in Rats Observed During

Exposure to Methyl Iodide by Exception

Weekly Summary of Occurrences of Gross

Signs of Toxicity Oeserved Before and

After Exposure to Methyl Iodide by Exception

Individual Animals Exhibiting Gross

Signs of Toxicity Before and After

Exposure to Methyl Iodide by Exception

Individual Clinical Signs

Summary of Chamber Environmental

Measurements

Daily Analytical Concentrations of

Methyl Iodide

Daily Miran Check (O.D) for the

Determination of Methyl Iodide

Distribution of Methyl Iodide in

the Test Chambers

Table 1 -

Table 3 -

Table 4 -

Appendices

Appendix I - Chamber Atmosphere Analysis

and Environmental Data

Exhibit II - Determination of Methyl Iodide

Concentrations in Air, Distribution and Environmental Data

Exhibit I - Procedure for Analysis of Methyl

Iodide Determination Using a

Mirane Infrared Detector

Table 4 -

Table 3 -

Table 2 -

Table 5 -

Table 1 -

Table 2 -
Appendix III - Gross Pathology

Text

Table 5 -

Table 6 -

Table 7 -

TCi.ble 8 -

Table 1 -

Table 2 -

Summary of Body Weight Data

Individual Body Weight Data

Change in Mean Animal Body Weights

Post-Exposure Relative to

Pre-Exposure

Animal Termination History

Summary Incidence of Individual

Gross Necropsy Alterations - Unscheduled Deaths

Summary Incidence of Individual

Gross Necropsy Alterations - Terminal sacrifices

..

Em. 810006

PAGE 5

I

I

Report page

49-50

51-58

59

60-67

68-78

69-70

71-74

75-78

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-

I NTRODtlC"l'ION

The purpose of this study was to determine tbe inbalation

toxicity of metbyl iodide vapor-aerosol in Sprague-Dawley male

and female rats exposed over an approxi~ate four-week period,

6 bours per day, 5 days per week for a total of 21 exposure

days. Groups of equal numbers of male and female rats were

each exposed during the study to target concentrations of 0

(Control, Group N), 0.140 (Low, Group 1), 0.420 (Mid, Group 2)

or 0.840 (High, Group 3) mg methyl iodide per liter of air.

Exposure concentrations were ba~ed on a previous investigation

(1). This study was conducted from March 11, 1981 to April 9,

1981, at the Environmental Health Laboratory (Ea~), 645 S.

Newstead Ave., St. Louis, Mo. 63110.

MATERIALS AND METHODS

Test Material

Methyl Iodide (Mel) is a clear to yellow liquid with a vapor

pressure of 6.6 psia at 700 p, boiling point of 42.4oC and

a molecular weight of 142. The test compound was received

from the Texas City Plant with unique identification,

500-201(2/9/81). The EHL substance identification code is

TalOOl3. It was reported to be 86.8\ Mel.

Test System: Animals and Husbandry

Eighty (40 males, 40 females) Sprague-Dawley rats (Crl:CD-

(SD)BR) were obtained from the Charles River Breeding Laboratories (Portage, Michigan). Test animals were beld and

observed in quarantine for 7 days prior to release for study.

Pive days prior to the start of the study animals weigbed

192-204 grams for males and 159-169 grams for females. On tbe

first day of stUdy, males were 48 days old and females were 53

days old. Eacb rat was uniquely identified by an eartag and

bar-coded cage card. Animals were randomly selected by the

computer and allocated on the basis of body weight into 4

groups of 10 animals/sex/group. This randomization precluded'

eaegistered Trademark of Charles River Breeding Laboratory, Wilmington, Massachusetts.

'.

EHL 810006

PAGE 6

. ,,~.

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.,-:,,-,:.. >,,> ,\/:,;;i>2:.~~~~,.'~-~.:/-:>;g~~

III I
significant differences among group mean body weights and

variability of individual body weights Within the groups.

Animals were individually housed in suspended stainless steel

wire mesh cages and given Purina Laboratory Certified Rodent

Chowe (5002) and tap water (St. Louis City, MO) ad libitum

except during the exposure period. Animal rooms-Were

routinely maintained at 70-740? and 35-60\ relative humidity

with a l2-hou: light/12-hour dark cycle.

Exposure System

Feur lO-m3 Rochester-type stainless steel chambers with

pyramidal tops and bottoms (Figure 1) were used for the

exposures. The rats were placed individually in wire mesh

cages suspended in an inhalation cage rack positioned on the

left side of the exposure chamber. Males were placed on o~e

side of the rack and females on the other. Rats were

positioned on the middle two tiers on the cage rack. The low

and mid-exposure atmospheres were generated by passing air

through MeI in a glass bubbler (Figure 2). MeI concentrations

were varied by altering the rate of airflow through the

bubbler. The high exposure atmosphere was generated by

metering pressurized MeI from a steel tank through a stainless

steel capillary tube into a Laskin-style nebulizer (2) located

at the top inlet of the exposure chamber. Test material

concentrations were varied by changing the flow rate of

material to the nebulizer. This was accomplished by changing

the pressure in the tank headspace above the test material

(Figure 3). Airflow in the chambers was maintained at a

constant flow of 1699 liters/min.

procedures for Monitoring Me! in the Inhalation Chamber

Nominal atmospheric concantration measurements were determined

daily for the low and mid- chambers following exposure. This

concentration was determined by dividing the amount of test

compound delivered to the chamber over the six-hour exposure

time by the total airflow through the chamber during the same

six-hour period. The amount of test material delivered to the

chamber was determined by calculating the difference between

the pre- and post-exposure weights of the bubbler. Nominal

-Registered Trademark of Ralston purina Company, St. Louis, MO.

EHL 810006

PAGE 7

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• •
atmospheric concentration Ir·"'c,- ·'.::t:ments were obtained at least

four times during each his~-~ev~l exposure. This nominal

concentration was determined by dividing the net amount of

test compound delivered to the chamber per unit time by the

airflow per unit time. Analytical samples were obLained four

times per cha~ber per day at a port in approximat~ly the

center of the exposure chamber door. The analytical

concentration of test material was determined by a Miran.

Infrared Analyzer. The analytical method is given in Appendix

I, Exhibit I. Quality control samples were injected into the

closed loop system of the Miran Infrared Analyzer. The

results of these injections were in relatively good agreement with the expected (Appendix I, Exhibit II, Table 3).

Distribution of the test matarial was determined three times

in each chamber during the study.

Animal Observations and Body weights

Animals were observed between the second and fifth hour of

each exposure, and a percentage estimate of animals eXhibiting hypoactivity, hyperactivity, tremors and/or convulsions,

irritation of the eyes and/or nose or breathing difficulties

was recorded. All animals were also individually examined for

gross signs of toxicity prior to and following each exposure

and at comparable times on non-exposure days. Weekly, each

animal was weighed and given a thorough examination for gross signs of toxicity.

Gross Pathology

A maximum of ten fasted animals/sex/group were thoroughly ex~ined following four weeks of exposure, euthanized and

necropsied. A maximum of five animals/sex/group were selected fo~ necropsy for each necropsy day. Animals that were found

dead and/or sacrificed in extremis were also given a detailed

necropsy examination. Necropsy included examinations of all

external areas and orifices, the cranial cavity, the external

surface of the brain, thoracic and abdominal cavities and the

subcutaneous cervical tissues and organs.

Statistical Procedures

Body weight data for animals of each group/sex were evaluated

by Dunnett's (two-tailed) Test (3) to detect significant

differences between means of treated groups and those of the

respective control group. Differences in tbe frequency of

lesions were determined using Kolmorogov-Smirnov one-tailed

test (4).

~Registered Trademark of the Foxboro Company, Norwalk, CT.

EBL 810006

PAGE 8

,
1.076

(0.097)

0.810

(0.049)

1.33

0.612

(0.076)

0.421

(0.030)

1.45

0.186

(0.031)

0.142

(0.012)

1.31

Low (0.14) Mid (0.42) High (0.84)

Nominal

(+ S.D.)

Analytical

(+ S.D.)

Nominal/Analytical

Comments on Study Conduct versus Protocol

Analytical concentrations of MeI in the high exposure level

were 0.810 mg/l and nct C.840 mg/l as specified. The nature ~f the generation system and the test material did not allow

the consistent ge~eration of 0.840 mg/l. Three males and 2

females in the high exposure group were necropsied the second necrop~y day. A ~aximum of 5 animals/group/sex/day are

usually l:ecropsied tcot' equal numbers/group/day although due to

animal deaths in the high exposure groups, these numbers were

reduced the second day (Sections 2.2.2 and 2.5.6). purina

Certified Rocent Chow ~nstead of Standard ChOW, is used

exclusively ac this f~cility and meets reqUirements for

nutrition and ~est~ictions for toxic contaminants (Section

2.5.5). Control chamber analysis was inadvertently not

measured the f~rst week of exposure (Section 2.8). It is the

opinion of the study director that these deviations did not

adversely affect the course of this stUdy.

--

EHt 810006

PAGE 9

Chamber Atmosphe:e Analysis and Environmental Data

Mean nominal and analytical values fot' the three exposure

levels of Mel were as follows:

)\lyp~\ concent}~t{;~~ (mg/l }·\AC.>

RESOLTS

Nominal exposure concentration values are shown in Appendix I,

Exhibit II, Table 1. Individual and mean analytical values

for samples obtained for each chamber/day are given in

Appendix I, Exhibit II, Table 2. There was good agreement

between target and analytical levels. The distribution of the

exposure atmosphere was determined to be uniform (> 94%) at

each of the exposure levels (AppendiX I, Exhibit II, Table

4). The ratios of nominal to analytical concentrations were

slightly higher than those of a true vapor (a true vapor would

have a value of 1). The daily means of environmental

measurements, such as temperature, relative humidity and

airflow were maintained throughout the study at 20.S-24.0oc,

21-58% and 1699 l/min, respectively (A~pendix I, Exhibit II,

Table 5).
Animal Observations and Body Weights

Hypoactivity, hyperactivity, irritation of the eyes and/or nose, breathing difficulties and tremors, twitches and/or convulsions were observed in animals during the period of animal exposure (Appendix II, Table 1). This was most prevalent in the high and to a lesser extent in the mid-exposure level animals. Irritation of the eyes, hypoactivity, nasal discharge, respiratory difficulties, abnormal pppearance of the skin and fur and a singular occurrence of tremors were observed in the pre- and post-exposure observation periods (Appendix II, Tables 2 and 3) in the high exposure group and to a lesser extent in some of the categories in the mid-exposure group. Similar observations were made at the weekly weigh periods (Appendix II, Table 4).

Significant decreases (p < O.Oll in mean body weight compared to those of the controls were observed throughout the study in high exposure male and female rats. Significant decreases (p < 0.01) were also observed in the mid-level male and female rats from Day 15 through the end of the study. Summary and individual body weight data for each exposure group versus those of the controls are given in Appendix II, Tables 5 and 6. The mean body weights of the high exposure animals were

midactually less at -Exposure Day 28 than Day 22. Animals of the and high exposure groups were not gaining weight at the same rate as comparable controls (Appendix II, Table 7). Five test material-related deaths (1 female was found dead, 2 males and 2 females were sacrificed in extremis) of the high exposure group also occurred the ~ast week of exposure. The animals sacrificed in extremis suffered large losses of body weight and were in generally poor physiological condition. Animal termination histories are shown in Appendix II, Table 8.

Gross pathology

The most consistent finding in the necropsies of four of the five unscheduled deaths was the gaseous distension of the gastro-intestinal tract (Appendix III, Table 1). One female was found dead and the carcass was destroyed before a necropsy could be performed. No compound referrable abnormalities were detected at the terminal sacrifice of the remaining animals

material

with the exception of the presence of a granular reddish/brown

males,

on the backs of the high exposure group animals (5/8 1/7 females) and a single 10,1{ exposure male and a suggestion of gastric difficulties (single C3se in each sex) in high exposure animals (Appendix III, Table 2). The suggestion of gastric difficulties may be an early~ndication

of stress but due to the low incidence, its relationship to compound toxicity cannot be determined.

EHL 810006

PAGE 10

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I

DISCUSSION AND CONCLUSIONS

Irritation of the eyes and/or Dose, hypoactivity and breathing

difficulties observed during exposure and non-e~posure periods

indicate that methyl iodide may be an irritant. The poor

physical appearance of the animals, as evidenced by the skin

and fur abnormalities, decreased weight gain or weight loss,

and death observed in the high exposure group animals are

indicative of the compromised physiologic condition of the

animals. These effects, excluding death, were seen to a

lesser extent in the mid-level animals, thus demonstrating a

concentration related phenomenon. The decrease in body weight

and the deaths observed late in the study suggest there may be

a cumulative toxic effect from repeated exposure to the

compound.

In conclusion, the results of this study indicate that the

highest -no-effect- exposure level for male and female rats

receiving six-hour exposures of methyl iodide during a

four-week period was 0.142 mg/l.

..

EHt 810006

PAGE 11

. - ~~.' ..:-
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REFERENCES

EHL 810006

PAGE 12

1. Blank, T. L. Inhalation Toxicity of Methyl Iodide (MeI)

to Sprague-Dawley Rats. MSL-26S0, EHL StUdy S10005.

ML-SI-OIS. 19S2.

2. Drew, R. T., Bernstein, D. M., and Laskin S. The Laskin

Generator. J. Tox. Environ. Health i 661-70 (197S).

3. Dunnett, C. W. MUltiple Comparison Procedure for

Comparing Several Treatments with a Control. Jour. Am.

Stats. Assoc. 1096-1121 (1955).

4. Siegel, s. Nonparametric Statistics for the Behavioral

Sciences. McGraw Hill Co. NY (1956).

dmo/4711A-12
Appendix I, Exhibit II

Table 1

Mean Daily Nominal Methyl Iodide Chamber Concentrations

Exposure Concentration (mg/I)

Day Low (0.14) Mid (0.42) High (0.a4)

1 0.144 0.711 0.892 + 0.017

2 0.162 0.533 0.989 +' 0.072

3 0.163 0.595 1.025 '+ 0.017

4 0.167 0.628 1.032 +' 0.009

5 0.177 0.567 1. 040 +' 0.000

6 0.163 0.490 1.040 +' 0.000

7 0.154 0.656 1.010 +' 0.000

8 0.154 0.570 1.018 +' 0.025

9 0.136 0.644 1.066 +' 0.010

10 0.149 0.623 1.040 + 0.000

11 0.206 0.453 1.031 +' 0.004

12 0.188 0.580 1.029 +' 0.014

13 0.203 0.602 1. 074 '+ O. 0.10

14 0.204 0.665 l.072 + 0.000

15 0.239 0.544 1.185 +' 0.076

16 0.216 0.541 1.246+0.080

17 0.217 0.675 1.143 + 0.016

18 0.212 0.763 1.108 +' 0.004

19 0.216 0.664 1.050 +' 0.050

20 0.209 0.687 1.350 + 0.228

21 0.232 0.670 1.148 +' 0.197

lC j: S.D. 0.186 + 0.031 0.612 + 0.076 1.076 .:!: 0.097

EHL 810006

PAGE 23

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B

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E

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EHL 810006

PAGE 33

1

2

3

4-8

9-18

19

20

21

Occurrence(a) of Specific Gross Signs of Toxicity in Rats

Observed During Exposure to Methyl Iodide by Exception

Appendix II

Table 1

Breathing

Difficulty

1-17

18-19

20

21

Bypoactivity

Exposure No.

1-4

5-13

14

15-17

18-19

20

21

Tremors, Twitches

and Convulsions

1-18

19-20

21

Hyperactivity
Females

'.

Exposure Levels

Males

iXL 810006

PAGE 34

Appendix II

Table 1 (Continued)

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I

Occurrence(a) of Specific Gross signs of Toxicity in Rats

Observed During Exposure to Methyl Iodide by Exception

1-3 C C

4-5 B B

6-16 C C

17-18 C C C C

19-21 C E C E

cal B = 25% of the observable animals have this effect.

C 50% of the observable a~imals have this effect.

D = 75\ of the observable animals have this effect.

E ... 100\ of the observable animals have this effect.

Exposure No.

Irritation of the Eyes

and/or Nose
THIS REPORT UAS OENERATED fROH DATA LOCKED THROUGII 5-AUG-82

Table 5

STUDY START [lATE I 

Of &ODY UEIOHT DATA (OH)

SPECIESI RAT SPRAGUE-DAULEY

SEXI HALE

DATE (198ill PRE- 18-HAR 25-HAR l-AF'R 7-APR

DROUP TARGET DOSE DAY OF BTUDYI TEST 8 15 22 28

------------------------------------------------------------------------------------._.----------------------

HH "EAN 199.0 287.8 328.6 364.3 387,1 HEDATIVE CONTROL BTD. nEV. 3.23 14.34 15.64 IB.45 20.19 SAHPLE SIZE 10 10 10 10 10

STUDY HUH&ERI 810006

DHEH NUHBERI

RTE OF AD"IHI INHALATION

"

- - - LEG END - - -

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DUNNETT'S TEST (TWO-TAILED) INDICATES STATISTICALLY SIGNIfICANT DIFfERENCE IP!.05)

••

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DUNNETT'S TEST (TWO-TAILED) INDICATES STATISTICALLY SIGNIFICANT DIFfERENCE PT -- BARTLETT'S TEST INDICATES STATISTICALLY SIGNIFICANT DIFfERENCE AHONO VARIANCES OF THE DIFFERENT GRO

NA -- DUHNETT'S TEST HOT APPROPRIATE FOR THIS GROUP/SEX/DATE

STUDY HUHBERt 810006 MONSANTO ENVIRONHENTAL HEALTH LA&oRATORY 

1H 0.14HG/LITER HEAN 199.1 286.2 322.6 357.8 376.7

TEST GROUP BTD, DEV, 3.18 17.30 27.67 35.73 38.73

HETHYL IODIDE SAHPLE BIlE 10 10 10 10 10

i! 2M 0.42HO/LITER HEAN 199.2 275.1 298.1U 313.4U 321.1U TE¥T DRoUP STD. DEli. 3.33 9.93 12.19 17.29 17.06 HE HYL IODIDE SAMPLE BIZE 10 10 10 10 10

...

.. 0

\DO 3M 0.84HG/LITER HEAN 198.9 257.9** 276.4** 284.8U 265.0U 0

en TEST GROUP 8TD. DEV. J.84 17.21 16.12 17.30 41.99

HETHYL IODIDE SAHPLE SIZE 10 10 10 10 10

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THIS REPORT WAS GENERATED FROH DATA lOCKEIJ THROUGII 5-AUO-B2

STUDY START D

STUDY HUHBERI 810006

DKEH NUHBERI

RTE OF ADHINI INHALATION

Table 5 (Continued)

SUHHARY OF WEIGHT DATA CGH)

SPECIESI RAT STRAIN/BREED I SPRAGUE-DAWLEY

SEXI FEHALE

DATE (1981)1 PRE- IS-HAR 25-HAR l-ArR 7-APR GROUP TAROET DOSE DAY OF 8TUDYI TEST 8 15 22 28

---------------------------------------------------------------------

.._-----------------------------------

Hf HEAN 164.3 205.1 220.4 234.0 242.68T

NEDATIVE CONTROL BTD. DEV. 3.40 6.64 9.17 12.16

SAHPLE SIZE 10 1010 10 10

IF O.14HO/LITER HEAN 164.5 208.4 224.6 241.1 249.7

TEST DROUP STD. DEV. 3.27 7.93 7.73 9.04 13.18

"ETHYL IODIDE SAHPLE SIZE 10 10 10 10 10

2F O.42HO/LITER MEAN 164.5 198.5 206.7n 217.2** 221.2**

DEV. 2.95 5.98 8.42 5.81 S PLE SIZE 10 10 10 10

...

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0 3F O. 84HD/L ITER liEAN 164.2 186.4** 191.2** 196.4** 188.0n

) aI TEST GROUP STD. DEV. 3.19 8.21 10.83 9.56 22.03 .1,,'- HETHYL IDIlIDE SAHPLE SIZE 10 10 10 10 8

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-- DUNNETT'S TEST (TWO-TAILED) INDICATES STATISTICALLY SIGNIFICANT DIFFERENCE IP!.05)

•• -- DUNNETT'S TEST (TWO-TAILED) INDICATES STATISTICALLY SIGNIFICANT DIFFERENCE BT -- BARTLETT'S TEST INDICATES STATISTICALLY SIGNIFICANT DIFFERENCE AKDND VARIANCES OF THE DIFFERENT D

NA -- DUNNETT'S TEST NOT APPROPRIATE fOR THIB OROUP/SEX/DATE

STUDY NUH8ERI 810006 HONSANTO ENVIRONMENTAL HEALTH