P.1 

OAR Box 1827 

Prepped by Charmelle Mathews 

Document Number: 

136) ll-A-6 

Docket Number: 

A-90-07 

Printed 11/19/2008 12:40:48 PM Sheet 136 of 147 
P.2 

' I ' I 

P384-164219 

Validation of Chemical and Biological 

Techniques for Evaluation of Vapors in 

Ambient Air/Mutagenicity Testing of 

Twelve (12) Vapor-Phase Compounds 

Research Triangle Inst . 

Research Tria-Kgle Park, NC 

Prepared for 

Health Effects Research Lab. 

Research Triangle Park, NC 

Mar 84 

..Air Qliiil — l 

!';'. AUG -91990 

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ED8U-16U21 S 

' ' ' ' < EPA-600/1-84-005 ! 

March 1984 

i 

VALIDATION OF CHEMICAL AND BIOLOGICAL TECHNIQUES FOR EVALUATION 

OF VAPORS IN AMBIENT AIR/MUTAGENICITY TESTING OF 

TWELVE (12) VAPOR-PHASE COMPOUNDS 

by 

Thomas J. Hughes 

Charles Sparacino 

Steven Frazter , 

Research Triangle Institute 

P.O. Box 12194 

Research Triangle Park, NC 27709 

i 

Contract No. 68-02-3170-082 

i 

i 

Project Officer 

Larry D. Claxton 

Genetic Toxicology Division 

HEALTH EFFECTS RESEARCH LABORATORY j 

OFFICE OF RESEARCH AND DEVELOPMENT 

US ENVIRONMENTAL PROTECTION AGENCY 

RESEARCH TRIANGLE HARK, NC 27711 

NATIONAL TECHNICAL ! 

INFORMATION SERVICE 

Ui :V'fyti:\: •• c:»Mt = ;| 

i??-s."M:::. .i ::;:. 

I 

P.3 
P.4 

T ' : 1 

if i 

-I 

..•-..-flWcrt, 

TECHNICAL REPORT OATA 

(Plcaie read Instruct:?TM on the rcverze before completing/ 

l. REPORT NO . 

EPA-600/1-84-005 

3. RECIP'LE.NT , S ACCESSION NO. 

4. TITLE ANO SUBTITLE 

Validation of Chemical and Biological Techniques for Evaluation of Vapors in Ambient Air/Mutagnnicity Testing of 

Twelve (12) Vapor-Phase Compounds 

5. REPORT OATE 

March 1984 

6. PERFORMING ORGANIZATION COOE 

7. AUTMORISI 

Thomas J. Hughes, Charles Sparacino and Steven Frazier 

8. PERFORMING ORGANIZATION REPORT NC 

9. PERFORMING ORGANIZATION NAME ANO AOORESS 

Research Triangle institute 

Post Office Box 12194 

Research Triangle Park, NC 27709-2194 

10. PROGRAM ELEMENT NO. 

11. CONTRACT/GRANT1 NO. 

68-02-3170-082 

12. SPONSORING AG-NCY NAME ANO AOORESS 

U.S. Environmental Protection Agency 

Health Effects Research Laboratory 

Genetic Toxicology Division, Genetic Bioassay Branch (MD68) 

Research Triangle Park, NC 27711 

13. TYPE OF REPORT ANO P6RIOO COyEREC 

14. SPONSORING AGENCY COOE 

EPA/600/11 

19. SUP'LEMENTARY NOTES 

16. ABSTRACT 

The overall objective of this research was to further validate Ames 

mutagenicity preincubation system for detection of vapors in ambient air. 

Parameters for detection of vapors in the Ames/Salmonella mutagenicity 

assay were investigated and 12 vapor-phase ccmpounds were tested for 

mutagenic potential in the above validated protocol with strains TA97, 

TA98 and TA100, bot'i with and without Aroclor-induced hamster and rat 

liver S9. The 12 vipor-phase compounds tested were: methylene chloride, 

formaldehyde, trichloroethylene, ethylene dichloride, epichlorohydrin, 

nitropropane, nitrobenzene, hydrogen sulfide, bucene, propylene, acetylene, 

and ethylene oxide. Ethylene oxide, epichlorohydrin, ethylene dichloride 

and methylene chloride were mutagenic. 

«.EY AOP"S »NO JCC'JMtsr ANALYSIS 

IS. ^:ST^IBJ7:0.- < iTy7Z'*tN 

Release ro Public 

! U-classified 

::. raicsi 

-nciassnie d 

EPA f."-< :j!- l :.H... *_;7) D« I vioul l5i''C « •» mcur t 
P.5 

& 

DISCLAIMER 

THE INFORMATION IN THIS DOCUMENT HAS BEEN FUNDED WHOLLY 

OR IN PART BY THE UNITED STATES ENVIRONMENTAL PROTECTION 

AGENCY UNDER CONTRACT NO. 68-02-3170 TO THE RESEARCH TRIANGLE 

INSTITUTE. IT HAS BEEN SUBJECTED TO THE AGENCY'S PEER AND 

ADMINISTRATIVE REVIEW, AND HAS BEEN APPROVED FOR PUBLICATION 

AS AN EPA DOCUMENT. 

ii 
P.6 

FOREWORD 

The many benefits of our modern, developing, industrial society are 

accompanied by certain hazards. Careful assessment of the relative risk of 

existing and new man-made environmental hazards is necessary for the establishment of sound regulatory policy. These regulations serve to enhance the quality 

of our environment in order to promote the public health and welfare and the 

productive capacity of our Nation's population. 

The complexities of environmental problems originate in the deep interdependent relationships between the various physical and biological segments 

of man's natural and social world. Solutions to these environmental problems 

require an integrated program of research and development using input from a 

number of disciplines. The Health Effects Research Laboratory, Research 

Triangle Park, NC and Cincinnati, CH conducts a coordinated environmental 

health research program in toxicology, epidemiology and clinical studies 

using human volunteer subjects. Widi ranges of pollutants known or suspected 

to cause health problems are studied. The research focuses on air pollutants, 

water pollutants, toxic substances, hazardous wastes, pesticides, and non-ionizing 

radiation. The laboratory participates in the development and revision of 

air and water quality criteria and health assessment documents on pollutants 

for which regulatory actions are being considered. Direct support to the 

regulatory function of the Agency is provided in the form of expert testimony 

and preparation of affidavits as well as expert advice to tho Administrator to 

assure the adequacy of environmental regulatory decisions involving the protection 

of the health and welfare of all U.S. inhabitants. 

F.Cordon Hueter.Ph.D. 

Director 

Health Effects Research Laboratory 

ill 
P.7 

Abstract 

The overall objective of this research was to further validate a 

® Tenax collection/Ames mutagenicity preincubation system for detection 

of vapors in ambient air. Recovery studies from Tenax were performed 

with three vapor-phase compounds that possessed a wide range of vapor 

pressures (i.e., epichlorohydrin, methylene chloride and nitrobenzene). 

Parameters for detection of vapor* in the Ames/Salmonella mutagenicity 

assay were investigated and 12 vapor-phase compounds were tested for 

mutagenic potential in the above validated protocol with strains TA97, 

c 

TA98 and TA100, both with and without Aroclor-induced hamster and rat 

liver S9. The 12 vapor-phase compounds tested were: methylene chloride, 

formaldehyde, trichloroethylene, ethylene dichloride, epichlorohydrin, 

nitiopropane, nitrobenzene, hydrogen sulfide, butene, propylene, 

acetylene, and ethylene oxide. 

Results suggested that: (1) recoveries of a model vapor-phase 

® 

compound from Tenax resin ranged from 22 tc 29 percent; (2) log-phase 

cells and the preincubation technique w„re the preferred methods for 

detection of vapor-phase mutagens; (3) the maximum linear mutagenic 

response was detected with 10 minutes preincubation, in full vials, with 

no shaking during preincubation; (4) solvent type and metabolic 

activation did affect mutagenic potential, and (5) ethylene oxide, 

epichlorohydrin, ethylene dichloride and methylene chloride were 

mutagenic. 

iv 
P.8 

Table of Contents 

Pace 

Foreword. Ill 

Abstract IV 

1.0 Introduction 1 

2.0 Materials and Methods 2 

2.1 Biological Techniques 2 

2.2 Cheiucal Techniques 3 

3.0 Results and Discussion 11 

3.1 Determination of Cell Type and Assay Conditions for 

Detection of Vapor-Phase Compounds in the Ames/Salmonella 

Mutagenicity Assay 11 

3.2 Toxic and Mutagenic Potential of Twelve (12) 

Vapor-Phase Compounds 19 

3.3 Recovery Studies of Vapors from Tenax® Resin 25 

4.0 Recommendations for Future Studies 26 

5.0 Project Summary 27 

5.1 Monthly Task Report (TR-1) 28 

5.2 Cost/Schedule Status Report (PR-2) 29 

5.3 Cumulative Budget Performance 30 

5.4 Completed Milestones for EPA Contract 68-21-3170-082 31 

APPENDIX 

Raw Toxicity and Mutagenicity Ddta of Twelve (12) 

Vapor-Phase Compounds in the toes/Salmonella Assay 
P.9 

1.0 Introduction 

Extractable organic mixtures from ambient air particulate matter 

are mutagenic in the Ames/Salmonella uiutagenicity assay. However, 

ambient air vapors have not been tested in the Ames/Salmonella assay 

because adequate chemical and biological techniques are not presently 

available for this analysis. In addition, it has been estimated that 

vapor-phase compounds (i.e., vapors are compounds that are gases at 

ambient temperature and pressure) may be 50-fold more mutagenic than 

ambient air particulate matter. Vapors from industrial centers and 

mobile and energy production sources are potential health hazards. 

Chemical and biological techniques must therefore be developed to 

evaluate the mutagenic potential of ambient air vapors. This research 

evaluated pure ambient *ir vapor-phase compounds and methods for their 

detection. 

This project further evaluated a Tenax collection/Ames mutagenicity 

preincubation system for detection of vapors in ambient air (Hughes et 

al., EMS, 1982). Pure vapor-phase compounds with a wide range of vaporpressures were utilized to evaluate the adsorption/desorption characteristics of these compounds from Tenax resin. Methods for the 

detection of vapor-phase mutagens in a preincubation modification of 

Ames/Salmonella mutagenicity assay were also investigated. 
P.10 

2.0 Materials and Methods 

2.1 Biological Techniques 

Salmonella typhimurium strains TA98 and TA100 were supplied by 

Dr. Bruce Ames, Department of Biochemistry, University of California, 

Berkeley, California. Stationary-phase cells were grown overnight (16 

hours) with shaking at 50 RPM; log-phase cells were grown on the day of 

the experiment by inoculating a fresh nutrient broth culture with a 

heavy inoculum of fresh overnight culture (1:10 dilution) and shaking 

the culture for 4 hours at 50 RPM. Incubation temperatures were 37°C (+ 

0.5°C). Plates were incubated for 48 hours, and colonies were counted 

with an Artex 880 automatic cell counter, calibrated by comparison with 

hand-counted plates and plastic disks with known colony counts. 

Solvents, liquid .ind gaseous compounds were of the highest quality 

available. The DMSO was spectrophometric grade and the ethanol was 100% 

absolute grade. The liquid test compounds were purchased from Fisher 

Scientific and the gaseous compounds were purchased from Matheson 

Scientific Company. Rat liver S9 was derived from male Sprague-Dawley 

rats and hamster liver S9 was derived from male Syrian Golden hamsters. 

Both rats and hamsters were induced with Aroclor 1254 at 500 mg/kg five 

days before sacrifice. 

Preincubation vials contained (per plate): 500 (Jl of 5% S9 mix, 

100 pi of bacteria, 100 pi of solvent with test compound, and 600 pi of 

overlay agar. Each vial couuained the contents for three plates. Halffull vials did not contain overlay agar. Preincubation was performed at 

37°C in sealed one-dram glass vials in a temperature-controlled exposure 

chamber that completely surrounded the glass vial (Isotemp , Fisher 
P.11 

Scientific). Toxicity was determined by plating approximately 1,000 

colonies on histidine-suppleraented agar. A viability index was 

calculated by comparing the number of colonies present on a dosed plate 

to a solvent control plate. A viability index below 50% (where greater 

than 50% of the bacterial population was killed) indicated excessive 

toxicity. A positive mutagenic response for TA97, TA98 and TA100 was 

defined as at least a two-fold increase in spontaneous revertants over 

background at two increasing dose levels. 

2.2 Chemical Techniques 

The five gaseous samples (hydrogen sulfide, ethylene oxide, 

acetylene, l-'»utene, aod propylene) were prepared in solutions for 

bioassay testing. ^ Ethanol/vas chosen as the solvent for all samples, 

except acetylene, because it offered excellent solubility characteristics 

for these gases, solution stability, ease of analysis, and has been 

reported to be relatively nontoxic to the Salmonella bacteria. The gas 

concentration in each sample was determined by gas chromatographic 

analysis. 

Standards were prepared by filling an evacuated 0.5 liter gas bulb 

with the appropriate gas and allowing the pressure to equilibrate to 1 

atmosphere. Samples were withdrawn through a septum in the bulb with a 

gas-tight syringe and chromatographed on a 6' chromosorb 102 packed 1/4" 

glass GC column by FI detection. The sample mass injected was determined 

fiom the density of the gas at STP as reported in the literature. 

Duplicate injections were made for each point and a response curve of at 

least three points was established for each species (see Figure 2.2-1). 
P.12 

i HYDROGEN SULFIDE 

M - BUTENE 

3 PROPYLENE 

« ACETYLENE 

5 ETHYLENE OXIDE 

(X-SCALE 25 UGS/DIV.) 

MRSS (MICRDGRRMSI 

Figure 2.2-1. Response Curves for Ames Assay Vapor-Phase Samples. 
P.13 

CHROMATOGRAPHIC CONDITIONS 

COLUMN - CHROMOSORB 102 l/«t" GLASS PACKED 

CARRIER - «iO ML/MIN NITROGEN 

T (INJ) - 200 DEGREES CENTIGRADE 

T (DET) - 200 DEGREES CENTIGRADE 

HYDROGEN SULFIDE: 

TEMP. - 50 DEGREES CENTIGRADE 

DETECTOR - FID 8 X E-12 

1-BUTENE: 

TEMP. - 120 DEGREES CENTIGRADE 

DETECTOR - FID 256 X E-ll 

PROPYLENE: 

TEMP. - 120 DEGREES CENTIGRADE 

DETECTOR - FID 512 X E-12 

ETHYLENE OXIDE: 

TEMP. - 120 DEGREES CENTIGRADE 

DETECTOR;- FID 512 x E-11 

ACETYLENE: 

TEMP. - 100 DEGREES CENTIGRADE 

DETECTOR - FID 128 X E-10 
aExcssseassc; 

Bioassay samples were prepared by diffusing the subject gas into 

the appropriate solvent. Due to analysis, solubility, and sample 

degradation problems associated with GHE (glycerol:water:ethanol ic a 

2:2:1 ratio [v/v]), ethanol was the preferred solvent for all the five 

gaseous samples, except acetylene, where acetone was utilized as the 

solvent. The solvent was placed in a gas washing bottle fitted with a 

frithed cylinder diffuser (Corning B1770) and the gas allowed to bubble 

through the solvent for approximately 10 minutes. A moderate positive 

back pressure was maintained to enhance dissolution. Ethylene oxide, 

1-butene, and propylene were prepared at 0°C to increase their 

solubility; hydrogen sulfide and acetylene were prepared at ambient 

temperatures. The samples were transferred to Teflon-capped vials, 

maintained at their preparation temperature, and immediately delivered 

for Ames biotesting. Aliqrots of each sample were removed for gas 

chromatographic analysis. 

Analysis of the samples was performed on a Chromosorb 102 packed GC 

column with FI detection. Triplicate injections were made for each 

sample and the concentrations determined from the mean (see Table 2.2-2). 
<9\ 

P.15 

Table 2.2-2. Summary of Gaseous Samples Prepared for 

Ames Bioassay Testing 

Sample 

Hydrogen : 

Ethylene < 

Acetylene 

1-Butene 

Propylene 

sulfide 

axide 

Concentration 

17.5 

111.3 

31.5 

1.3 

6.3 

(Mg/^D Temp< 

c 

»rature 

15 

0 

20 

0 

0 

(°C) Solvent 

Ethanol 

Ethanol 

Acetone 

Ethanol 

Ethanol 
r « 

i 

For the desorption studies of vapors from Tenax resin, glass 

cartridges (21 x 3 cm) were loaded with ca. 12 grams of Tenax resin. 

Each of three cartridges was spiked with 260 ng methylene chloride and 

200 ng epichlorohydrin using a permeation system. The cartridges were , 

stored approximately 48 hours at -17°C prior to desorption.. They were 

removed from storage and immediately desorbed at 250°C. The desorption 

apparatus (Figure 2.2-2) consisted of glass, Teflon or stainless steel 

surfaces and was configured in such a manner as to minimize unnecessary 

dead volume. Flowing helium carrier gas (40 ml/min) was used to pass 

the descrbed material through a 3-way valve (position AB) to a coiled 1 

meter length of Teflon tubing (1.35 min. ID) which was partially 

immersed in liquid nitrogen (LN_). Purging was continued for 30 minutes 

to ensure complete removal and entrapment of the volatiles. At the end 

of this period the LN. bath was replaced by an ice/H.,0 bath. After 

equilibration (~5 minutes) the 3-way valve was manipulated (position BC) 

to stop helium flow and to bring the syringe in line. The trapped 

volatiles were collected in 1.0 ml GltE and discharged from the syringe 

through the coil. The GHE solution passed into a 1 ral collection vial 

which was immersed in an ice/H,0 bath. The solution WJS immediately 

analyzed by purge and trap GC with Hall electrolytic conductivity 

detection. System and solvent blanks were prepared and analyzed with 

the samples. 

Standards were prepared by spikinp aliquots of vehicle (CKK) with 

epiclilorhydrin and methylene chloride stock solutions in ethanol. Thrtf-e i 

point standard curves were determined (or 93 ng to 310 ng injected lor 

methylene chloride and 10S ng to 362 ng injected for t\.i thlorohydr in. I 
' I ' I 

P.17 

Gas chromatography was performed on a Fisher-Victoreen series 4400 

gas chromatograph fitted with an aqueous sample purge and trap apparatus. 

A 0.5 ml aliquot of tue sample or standard was diluted to 4.0 mis with 

deionized, purged water, transferred to tbe purge apparatus and purged 

onto a Tenax GC trap for 12 minutes with nitrogen carrier gas. The 

Tenax trap was then heated to 300°C and the volatiles passed onto a 6' 

1% SP-1000 on Carbopak B column with 31 tal/min nitrogen carrier flow. 

The temperature program was 60°/2 minutes to 220°C at 6°/min. The 

compounds were detected on a Tracor 700 Hall electrolytic conductivity 

detector and the peak area electronically integrated. The minimum L0D 

(limit of detection) for epichlorhydrin and methylene chloride was 5 ng. 
ft* In 

1 ml Syringe 

Tenon 

Cartridge 

I Mlgfi Tamp. 

I y Dotorpiion 

F Dwvic« 

JWsy H«out 

LN, w KE/M,0 

Beth 

I fot/HtO 

Beth 

Fir.urc 2.2-2 . Srhrm.it lc of l!,en».,l desorpt ion/vapor collection 

device . 
P.19 

3.0 Results and Discussion 

3.1 Determination of Cell Type and Assay Conditions for Detection 

of Vapor-Phase Compounds in the Ames/Salmonella Mutagenicity / 

/ 

Assay 

Parameters investigated for detection of vapors in the Ames/ 

Salmonella mutagenicity assay included: toxicity aod mutagenicity 

testing of styrene oxide (a vapor-phase mutagen) with log- and 

stationary-phase cell- of TA100 with the preincubation and plate incorporation techniques, mutagenicity testinj of methylene chloride in the 

plate incorporation and preincubation techniques, the effect of headspace 

volume and different types of shaking (during preincubation) on the 

mutagenic potential of vapor-phase mutagens (styrene oxide and methylene 

chloride). The effect of solvents and metabolic activation on the 

i 

mutagenic potential of vapor-phase mutagens, and the effect of preincubation time (frcm 10 to 60 minutes) at 37CC on the mutagenic potential 

of styrene oxide were also investigated. The results from these experii 

ments were fully discussed in the progress reports for this task, and 

will be summarized here. 

Results suggested that log-phase cells and the preincubation 

procedure were the core sensitive technique for detection of vapor-phase 

mutagens. Two experiments demonstrated that the preincubation procedure 

permitted detection of styrene oxide (SO) mutagenicity at doses similar 

to those in the plate incorporation procedure, and log-phase cells were 

more sensitive to the mutagenic effects of SO than stAticn-iry-phasc 

cells of Salmonella strain TA1C0 (sec Figures 3.1-1 and 3-.1-2). Toxici'y 

w.-»s significantly higher in the preincubation procedure- than in the 

11 
P.20 

1.290 

1.200 

1.140 

1.110 

1.030 

1.030 

970 

910 

860 

800 

740 

710 

660 

600 

540 

490 

430 

370 

310 

260 

200 

-

' \ 

- \ 

\ f 

- 1 /)\ 

- \ If \ 

- 1 t / \ 

- y / \ 

*" i • i * 

/ _ 

/ 

/ 

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*——^^ ^ -

" *——-^ 

-

-

-

-

-

-

'N. 

^ v 

^v _ 

X. 

1 ^v"* 

X 

v 

TS 

c 

- 74 >, 

100 

98 

94 

90 

86 

82 

78 

74 

70 

66 

62 

54 

50 

46 

42 

40 

38 

36 

9 

100 200 300 400 SOO 600 700 600 900 

Conctntntion of Sryrtne Ondt Ug/plm) 

1.000 

Figure 3.1-1. Plate incorporation of styrene oxide with log- and 

stationary-phase cells of Salr.onel la strain TAlOO 

(Expericier.t 2). Symbols are: Mutagenicity to 

stationary-phase cells (• •) ; toxicity to 

stationary-phase cells (0 0) ; mu:.agcnicity to 

log-phase (A A) , toxicity to log-phase cells 

(.1 1^. Spontaneous backgrounds (mean of throe 

p.-ites) were: log-phase cells, 221 revertants and 

stationary-phase cells, 204 revertants. 
P.21 

600 700 800 

Concentration ol Styrene Oxide (jig/plate) 

1.000 

Figure 3.1-2. Preincubation of styrene oxide with log- and stationaryphase cells of Salmonella strain TAlOO (Experiment 2). 

Symbols are: Mutagenicity of stationary-phase cells 

(• •) ; toxicity of stationary-rphase cells (0——0) ; 

mutagenicity of log-phase cells (A A); toxicity of 

log-phase cells (£ A). Spontaneous backprounds (mean 

of three plates) were: log-phase cells, 276 revertants, 

and stationary-phase cells 260 revertants. 

13 
plate incorporation procedure and log-phase cells were more sensitive to 

the toxic effects of SO (compare Figures 3.1.-2 and 3.1.-2, 

respectively)." The higher toxicity in the preincubation procedure with 

log-phase cells indicated a closer association of bacteria and test 

compound, which was one of the major advantages of the liquid suspension 

preincubation technique when testing vapoi'-phase mutagens. When preincubation times of 5 to 30 minutes (in 5-minute intervals) were evaluated, 

the maximum linear mutagenic response of SO with TAlOO was detected at 

10 minutes (see Figure 3.1-3). An initial experiment evaluated preincubation times from 10 to 60 minutes and results suggested that the more 

linear preincubation time for vapor-phase mutagen detection was below 30 

minutes at 37°C (data not shown). 

The effect of headspace volume and shaking methods on the mutagenic 

potential of SO in the preincubation assay was also investigated. 

Log-phase cells of TAlOO were preincubated in one-dram vials that were 

either full or hjlf-full. Both types of vials were preincubated for 30 

minutes at 37°C and shaken by three different types of shakers (wrist, 

i 

rotator and roller drum). Results were similar with either full or 

half-full vials and any type of shaking; headspace volume and method of 

shaking did not significantly increase the mutagenic potential of SO. 

In addition, results with log-phase cells and full vials that were not 

shaken;produced a significantly greater number of revertonts than did 

any type of shaking. Consequently, the most sensitive method for 

detection of vapor-phase mutagens was established as preincubation with 

log-phase cells in f;11 vials that were not shaken during preincubation. 

i 

This protocol was utilized in the testing of the 12 vap0r-ph3.se mutagens 

14 
P.23 

7 " » 

100 200 300 400 WO 600 700 

Styrene Onitfe (ug/plite) 

BOO 300 

Figure 3.1-3. Effect of varying preincubation time on the mutagenic 

potential of styrene oxide to TAlOO; vials verc full 

I and preincubated at 37°C in a dry veil bath vith no 

shaking. Log-phase cells, grown on the day of the 

! experi=ient were utilized. Symbols are: 0 min (plate 

incorporation (^ 0) ; 5 r,in (• • ); 10 rr.in 

; (g Q). 15 j.in (A k). no min (0 0); 25 

| (Q Q) ; and 30 min (i 1) preincubation time. 

Spontaneous background rates averaged from 150 to 

200 revertants/plate. 

15 
I i 

P.24 

discussed in Section 3.2 of this report. Since these experiments were 

performed with only one chemical (SO), additional experiments should be 

conducted to confirm these findings with additional Vdpor-phase compounds 

and with S9 metabolic activation. 

The effects of solvents and S9 metabolic activation on the mutagenic 

potential of vapor-phase mutagens was also investigated. SO and 

methylene chloride (MC) were tested with TAlOO in the preincubation 

procedure (full vials at 37°C for 10 minutes with no shaking during 

preincubation), with and without the addition of 5% hamster liver SS 

(HLI) in the following solvents: GHE, DMSO and ethanol. Log-phase 

cells, which were regrown from a stationary-phase culture on the day of 

the experiment, were utilized. Results indicated that the addition of 

S9 inhibited the mutagenic potential of SO and slightly increased the 

mutagenic potential of MC (Figure 3.1-4). The effect of DMSO, GHE and 

ethanol on the mutagenic potential of SO without S9 addition is 

summarized in Figure 3.1-5. The three solvents demonstrated similar 

mutagenic responses, but mutagenic activity was highest in DMSO followed 

by GHE. , Mutagenic activity was depressed but present with ethanol. The 

magnitude of the mutagenic response may be due to the ability of the 

solvent to permeate cell membranes. A glycerin:ethanol solvent (2:1 

ratio v/v) may be preferred over GHE, since the addition of water could 

inhibit compound solubility, and may therefore inhibit the mutagenic 

response of water insoluble vapor-phase mutagens. These results 

suggested that the solvent system for the Tenax adsorption/desorption 

unit should be investigated further vith regard to the effect that 

solvents may have on mutagenic potential of vapor-phase mutagens. 

16 
P.25 

0 50 100 250 500 

Concentration [pg/plate) 

1.000 

Figure 3.1-4. Effect of solvent (GHE) and S9 metabolic activation on the 

mutagenic potential of styrene oxide and methylene chloride 

with T.AIOO. Symbols are: •—styrene oxide + S9; A—styrene 

oxide - S9; o—methylene chloride + S9; A—methylene chloride 

- S9. Spontaneous background values were: 154 revertants 

with S9 and 159 revertants without S9. 

17 
.r 

ir 

P.26 

•.-: : .' J 

DMSO 

GHE 

0 50; 100 250 500 

Concentration [/jg/plate] 

Ethanol 

1.000 

Figure 3;. 1-5. Summary Graph of the Testing of Styrene Oxide with TAlOO, 

Without S9 Addition. (See Figures 1-3 for Details of 

I Experiment.) 
P.27 

Vapor-phase compounds also should be tested both with and without S9 

addition. 

3.2 Toxic and Mutagenic Potential of Twelve (12) Vapor-Phase Compounds 

The preincubation technique developed above was utilized to test 

twelve (12) vapor-phace compounds: m.-thylene chloride (MC), formaldehyde 

(FM), trichloroethylene (TRC), ethylene dichloride (EDC), epichlorohydrin 

(EC), nitropropane (NP), nitrobenzene (NB), hydrogen sulfide (HS), 

butene (BT), propylene (PP), acetylene (AC), and et,vylene oxide (EO). 

Samples were evaluated for toxicity at five doses before 

mutagenicity testing with and without addition of rat liver S9. 

t 

(Methylene chloride was not tested for toxic potential because previous 

experiments with this chemical had established the nontoxic doses, and 

the five gases tested [H_S, butene, propylene, acetylene and ethylene 

oxide] were not tested for toxic potential due to difficulty of sample 

preparation.) The raw data for the toxicity testing of the remaining 

six compounds are contained in the Appendix and are summarized in Table 

3.2-1. Formaldehyde was highly toxic at doses greater than 100 

yg/plate; the other five compounds were less toxic. 

Mutagenicity testing parameters were: ?og-phase cells of TA97, 

TA98 ar.d TAlOO (regrown on the day of the experiment from an overnight 

stationary-phase broth culture); five doses, triplicate plates/dose, and 

100 pi of solvent/plate. Acetylene vas diluted in -cetonc; H„S, butene, 

propylene and ethylene oxide was diluted in ethanol, and the remaining 

compounds were diluted in GHE (glycerin:water:ethanol) in a 2:12:1 ratio 

® 

(v/v). GHE has been established as the solvent-of-choice for the Tenax 

desorption unit/Ames preincubation system (EPA contract . -02-2724). 

19 
5? 

P.28 

Table 3.2-1. Summary of the Toxic Potential of Six Vapor-Phase 

Compounds 

Doses Tested Nontoxic Doses 

((jg/plate) -S9 +S9C 

b ! 

Formaldehyde 1000, 500, 250, 100 250 

100, 1.0 

Trichloroethylene 10,000, 3,333, 1000 333 

1,000, 333, 100 

Ethylene Dichloride 1000, 500, 250, 1000 1000 

100, 50 

Epichlorohydrin 10,000, 3,333, 1,000, 3,333 3,333 

Nitropropane 10,000, 3,333, 1,000, 10,000 3,333 

333, 100 

Nitrobenzene 10,000, 3,333, 1,000, 3,333 3,333? 

333, 100 

3 

TA100, triplicate plates/dose, 200-1000 colonies on histidine-suppleraented 

media. j 

Dose at which the viability index was greater than 50%. j 

CRat liver S9 from Sprague-Dawley rats induced with Aroclor 1254. 

Lowest nontoxic dose was chosen for highest mutagenicity dose level. j 
Compounds were tested with 5% male Syrian Golden hamster liver S9 (HLI) 

and male Sprague-Dawley rat liver S9 (CSD) and without S9 addition. The 

protocol conditions were preincubation for 10 minutes at 37°C in full 

vials with no shaking during preincubation. 

Raw data and dose response graphs for the mutagenicity testing of 

the 12 vapor-phase compounds are contained in the appendix. The data 

are qualitatively summarized in Table 3.2-2. Methylene chloride, 

ethylene dichloride, epichlorohydrin and ethylene oxide were mutagenic. 

Approximate initial slope values for these four compounds are contained 

c 

in Table 3.2-3. Criteria for a positive mutagenic response on strains 

TA97, TA98 and TAlOO were defined as a mutagenic response at two 

increasing dose levels that was at least twice the spontaneous background 

values. From evaluation of the approximate slope values, the positive 

mutagenic vapors can be ranked from high to low as follows: Ethylene 

oxide > Epichlorohydrin > Ethylene dichloride >> methylene chloride. 

Ethylene oxide and epichlorohydrin were strong direct-acting base 

substitution mutagens, ethylene dichloride was a moderate direct-acting 

base substitution mutagen, and methylene chloride was a weak directacting jframeshift mutagen. Addition of S9 did not increase nutagcnic 

activity, and in the cases of ethylene dichloride and ethylene oxide, 

actually decreased mutagenic activity significantly. 

It should be noted that high test concentrations of the gaseous 

compounds (H0S, butene, propylene, acetylene and ethylene oxide) were 

difficult to obtain in liquid suspension. The highest mutagenicity test 

dose was limited by the solubility of the test gas in the test solvent 

(acetone for acetylene and ethanol for the other four gases). For 

21 
P.30 

Table 3.2-2. Mutagenicity of Twelve Vapor-Phase Compounds 

Vapor Compound TA97 TA98 

-S9 RLI HLI -S9 RLI HLI 

TAlOO 

-S9 RLI HLI 

Methylene Chloride 

(GHE) 

Formaldehyde (GHE) 

Trichloroethylene (GHE) 

Ethylene Dichloride (GHE) -

Epichlorohydrin (GHE) ••* 

Nitropropane (GHE) 

Nitrobenzene (GHE) 

H2S

b (ETOH) 

Buteneb (ETOH) 

Propylene5 (ETOH) 

Acetylene (acetone) 

Ethylene Oxideb (ETOH) 

V* W+ U+ 

+•• ••• •••-* 

++ 

Preincubation, 37°C, 10 minutes, full vials, no shaking, Isotcmp. 

b. 

Gas at room temperature. 

Ranking 

TA97 

TAlOO 

TA98 

legend: 

W

+ 

2-3" 

2-3 

2-4 

+ 

3-5 

3-5 

4-10 

+• 

5-8 

5-8 

10-25 

++• 

>8 

>8 

>25 

-Mutagenic ratios at greatest response. 

v revertants vith sarr>le. Mutageni s c ratio •» -?, . : ,—*—— V rtvort.ints in solvent 
P.31 

Table 3.2-3. Approximate Initial Slope Values for Mutagenic Vapor-Phase 

Compounds 

TA97 TA98 TA10G 

-S9 RLI HLI -S9 RLI HLI -59 RLI HLI 

Methylene Chloride - 0.05 0.08 0.08 -

Ethylene Dichlcride ----- - 0.60 - * 

Epichlorohydrine 0.92 0.86 0.82 - - 0.03 2.35 2.01 1.63 

Ethylene Oxide - - - - - - 5.12 - 3.18 

23 
example, the highest test dose for butene was 130 pg/plate, for acetylene 

was 31 pg/plate and for ethylene oxide was 111 pg/plate. Propylene "as 

soluble in ethanol and the highest dose obtained was 626 pg/plate. H.S 

was soluble in ethanol at a high dose of 1,750 |Jg/plate. Additional 

experiments should be conducted with these vapors in the gaseous state, 

where much higher dose levels can be attained. Further testing with 

ethylene oxide should prove beneficial in the comparison of mutagenic 

testing procedures for vapor-phase mutagens. Ethylene oxide is a gas at 

room temperature, and is also mutagenic in liquid suspension. Comparison 

of data of ethylene oxide in solution and if: the gaseous state should 

provide an insight as to the effects of liquification (as performed with 

the Tenax desorption unit) on the mutagenic potential of gases. It may 

be possible to cool the vapors after Tenax desorption and exposed them 

directly to the bacterial test system. 
3.3 Recovery Studies of Vapors from Tenax Resin 

Recovery of epichlorohydrin was 22% and 29% for two of the spiked 

cartridges, but was not detected in a third. In a previous study (EPA 

contract No. 68- 02-2724) of two compounds chemically related to 

epichlorohydrin, similar recovery was observed (ethylene oxide, 20% and 

propylene oxide, 28%). Methylene chloride was not detected in any of 

the three samples. 

The poor recovery of these compounds is probably due to several 

complementing factors. Epoxides will readily hydrolyze to glycols in 

solutions of acidic pH (GHE has a pH of 5.0) or at elevated temperatures 

in the presence of moisture, thus some loss of epichlorohydrin may be 

expected. The failure to recover methylene chloride may be due to poor 

solubility in the vehicle. From these observations, it is suggested 

that pure ethanol be used as the recovery vehicle for cryotrapped 

volatiles. Some changes in desorption and purge times could be 

implemental in future studies to increase overall recovery. 

The recovery study of nitrobenzene was not completed. Several 

problems were encountered with the GC analysis of this compound in the 

GHE vehicle and an acceptable limit of detection was not obtained for an 

environmentally realistic sample. 

25 
P.34 

4.0 Recommendations for Future Testing 

1. Evaluate plastic bags as exposure chambers. 

2. Test vapor-phase compounds with the new Salm^.°lla testestrain, TA102, which is effective in detecting o.idative mutagens. 

3. Radiolabel vapor-phase compounds t> evaluate the amount of 

vapor that is actually absorbed by the bacteria and test system. 

4. Evaluate ths effect that solvents, shaking an* amount of 

headspace in the preincubation vials have on the mutagenic potential of 

potent vapor-phase mutagens. 

26 
P.35 

5.0 Project Summary 

5.1 Monthly Task Report (TR-1) 

5.2 Cost/Schedule Status Report (PR-2) 

5.3 Cumulative Budget Performance 

5.4 Completed Milestones for EPA Contract 68-21-3170-082 

27 
P.36 

MONTHLY TASK REPORT (TR-1) 

FOR PHOJECTS SUPPOHTEO CY 

INDUSTRIAL ENVIRONMENTAL RESEARCH LABORATORY 

OFFICE OF RESEARCH & DEVELOPMENT/ENVIRONMENTAL PROTECTION AGENCY 

RESEARCH TRIANGLE PARK. NJRTH CAROLINA 27711 

Validation of Chemical and Biological Techniques for Evaluation of Vapors in 

TOATDTITLE Ambient Air/Mutaeenicitv Testing of TVPIVP (,?. ya p ,^-pv, n g o r^^., ^ .. 

COMPANY,nivisinw Research Triangle Institute , Chemistry and Life Sciences Group 

CONTRACTOR'S TASK LEAOER (FOR THIS TO/TD) 

NAME. Thomas J. H-ighes 

TITLE Genetic Toxicologist 

«ni««« RTI, Bldg. 3 

?nnnE 541-6148 

Research Triangle Park, NC /ipeonF 27709 

EPATAS:'. OFFICER 

(FOR THIS TO/TO) _ Dr. Larrv Claxton 

CONTRACTORS PROJECT MANAGER(FOR OVERALL CONTRACT) 

NAME Dr. FnrPst Mivnn 

TITLE Manager 

AnnnFSS RTI/PCED, Bldg. 9 

PHONE 541-5917 

Research Triangle Park, NC ?IPCODE ?77no 

EPAPROJECTO?FICER 

(FOR OVERALL CONTRACT! Alic e D. Cagnon 

6,8 2 ,113 , 1,7 

CONTRACT NUMBER 

598 

. ° 0,8, 2 

, , M M D D Y Y , , 

0 ,6 3_o0_ 8 -3 0 

TO/T D 

NUMBER 

t>. PORT PERIOD 

CNOINC OATE 

TASK START OATC 10/15/8 2 

W « , TrnaT , 06A30/83 

598 598 

CUMULATIVE MANHOUR S 

EXPENDED TO LIATC 

CURHENTTAOCET ESTIl.'iT'. AT 

COW>l.'.TiON 

VARIANCE 

AT CCi'UlIlO N 

PF'.OGHESS DESCRIPTION A3.' .'RACT: 

\% (KEYPUNCHED ANO PRINTEO'IN THE IERL/RTP MONTHLY STATUS REPORT.) 

1 0'1 Results suggested that: (1) recoveries of a 

| 02| model vapor-phase conpound from Tenax resin 

[~QT] ranged from 22 to 29%; (2) lop-phase cells and 

| 04| the preincubation technique were the preferred 

| 05) methods for detection of vapor-phase mutagers; 

77 >[ :t 

-i 

06 (3) the maximum linear mutagenic response was 

07 

08 

detected with 10 minutes preincubation, in full 

vials, with no shaking during preincubation; 

C9l (4) solvent type and metabolic activation did 

[ 10| effect mutagenic potential, and (5) ethylene 

| n | oxide, epichlorohydrin, ethylene dichloride 

[12] and methylene chloride were mutagenic. 

it « S b 

: A NOTE TO THE AUTHOR: 

UltTCC-WrttTl.liOSMtl'MC 

s«'i;:ii«.( «oi -;.it-f.i 13 

AV2:3 L. km 'e l ISlLC&.i, 

(11 Tti! JtC'll l»»l?l 

K'I.W I I*II«*!:UI M i 

Ui TH | tuif.«-'.\.i;<( a 

l*»i u «•!» • »f , 

Ill C«U.S--IOl l 

l«l>r. v>t»»i u lii. i 

(li^ii^r^illil'M^i j 

ui s: «MI«I : fc0U!:*> 

|i:->rt:u v-i» » ;i:: r 

l";12» ».'U .rf (A* ll »>:f >.!] 

THE rcuuo.vr.." . SWPOL I CAK 

BE KLVPUN .-><'. r>: 

• • t • :• 1 0 = t 

MATERIAL WHICH RUNS OVER 1 HE I J LIN t LIMIT WILL I'L THUfiC A I f.O 

THIS I'nBM IS VI bl !•> rpR INPODMATiON TflANirf l f'^LV . IT IS NOT I TIT r.nctr. TO rtr.ry t. ir. 

THE COMPREHENSIVE PROGRESS DESCRIPTION RtCJwItACD O* TH* I-"" POL'J.'CT OFH:tf l 

FOR USE BRANCH CHIEF 

BY INITIALS: 

ItT.L/HTP 

EPAinrpi 307 

1. INCLUDE AS MAJOR ITEM IN IE RL.'UTP ?'0NT111. V REF0RT.

 V<-S Cj'JOC D 

2. PROJECT REQURES TOP f.'AMGEI.'EM AHMiilO:; .

 YE* £03 '^'ZJ 

3. SEE COMMENTS 0!i CACK FOR IARO^ATORY MANAGI VE NT. VtS CZ3 N 0 C D 

2S | Reproduced Irom <L . ^ 
COST/SCHEDULE STATUS REPORT (PR-2) 

_ Researc h Triangl e Institut e ,, . , . , „„Ar, „„ . 24,96 4 

Contractor name: Estimated contract cost ,» ' . 

r> , „ , (excluding feel 

EPA project »»ir„ -

 Pr

-

 Lfirr y "axto n 

M M D D Y Y 

6 M 0 . 2 3 , 1 |7i P 

Contr je t fJo. 

k .^l

 i2EiI UL i i i S 181 C 13 

Thomas J . Hughes , Geneti c Toxicol . 

Contractor project manager name, title 

Signature 1 / (J Date 

T.vA/71) No. End ol reporting period 

Performance data* 

( I I 

Work breakdown structure 

Na. Name 

Chemical 

validation 

Ami; s 

validation 

Mutagenicity 

studies 

Reports 

Contract 

Cumulative cost to date (SlOQO's) 

Budqet'd costs 

(21 

Work scheduled 

(BCWSI 

6.0 

9.0 

9.0 

0.7 

0.5 

General and administrative 

Management resevr 

(3) 

Woik p-Pilo'merl 

(DCWP) 

6.0 

8.8 

9.0 

0.7 

0.5 

Actual costt 

ol 

work pei lor med 

(ACWP) 

6.0 

8.8 

9.0 

0.7 

0.5 

Variance 

(51 

Schedule 

0 

0 

(6) 

Cost 

0 

0 

^C 

Cost at completion (SlOOO's) 

(7) 

BudQ-Med 

3.4 

5.7 

11.5 

2.0 

2.4 

(8) , 

Latest 

revised 

estimate 

FT 

(9) 

Variance 

+2.6 

+3.1 

-2. 5 

-1. 3 

-1. 9 

17(23 ?-1 ?5 

Total 

^excluding Ice) 

2C 27 21 ?] ia]}\ 17 J3JJ4 a^lC 17 39 Sli-.v 

• 0 

4JJ44 41 44 47 «t 41 JJ 14 

r I.I 

DSUO Ill l 

FT 

c 

34 It U it I I tf 

g 

aAII costs are exclusive ol lee. 

7«77 7| i 
«,-«.-•. . 

-

. " " 

5 3 Cumulative Budget Performance (June 30, 1983) 

EPA Contract No. 68-02-3170-082 

26 

24 

22 

20 

18 

(1000s) 16 

14 

12 

10 

8 

6 

4 

r

"7 

/ / 

/ / 

/ / 

/ / 

r / 

/ / 

/ / 

/ / 

? / 

/ / 

/ / 

/ / 

/ / 

; / 

/ / 

/ / 

/ / 

/ / 

/ / 

i / 

i / 

i / 2

 7 

i/ 

"'" "N "" D J F M A M J 

1982 1983 

Time 

30 

... , 

i 

i 

i 

i 

i 

• 

i 

i 

i 

! 

1 

! 

j 

1 

i 
5.4 Completed Milestones for EPA Contract 68-21-3170-082 

June 30, 1983 

P.39 

Ml Work Plan submitted 

M2 Project organizational meeting with 

EPA Project Officer 

M3 Chemical validation studies started 

M4 Ames/preincubation studies started 

M5 Ames/preincubation studies completed 

M6 Compounds tested for mutagenicity in 

preincubation assay 

M7 Chemical validation studies completed 

M8 Final Report completed 

Date 

Planned 

10/21/82 

10/28/82 

11/1/82 

11/1/82 

1/15/83 

1/15/83 to 

5/30/83 

5/30/83 

6/30/63 

Date 

Achieved 

10/19/82 

10/28/82 

11/1/82 

11/1/82 

2/4/83 

3/18/83 

5/22/83 

6/30/83 

31 
P.40 

Appendix 

Raw Toxicity and Mutagenicity Data of Twelve (12) 

Vapor-Phase Compounds in the Ames/Salmonella Assay 

32 
P.41 

Table of Contents 

Pajtje 

Computer Legend 34 

Toxicity Data 

Toxicity of Formaldehyde (FM) 36 

Toxicity of Trichloroethylene (TRC) 39 

Toxicity of Ethylene Dichloride (EDC) 42 

Toxicity of Epichlorohydrin (EC) 45 

Toxicity of Nitropropane (NP) 48 

Toxicity of Nitrobenzene (NB) 51 

Mutagenicity Data 

Mutagenicity of Methylene Chloride (MC) 54 

Mutagenicity of Formaldehyde (FM) 66 

Mutagenicity of Trichloroethylene (TRC) 78 

Mutagenicity of Ethylene Dichloride (EDC) 90 

Mutagenicity of Epichlorohydrin (EC) t 102 

Mutagenicity of Nitropropane (NP) 114 

Mutagenicity of Nitrobenzene (NB) 126 

Mutagenicity of Hydrogen Sulphide (HS) 138 

Mutagenicity of Butene (BT) 150 

Mutagenicity of Propylene (PP) 162 

Mutagenicity of Acetylene (AC) 172 

Mutagenicity of Ethylene Oxide (EO) 186 

33 
P.42 

Legend for Computer Output 

EXP DATE = Date of the experiment. 

Test type = Plate incorporation, spot, preincubation, or tgar well test 

Dilution = Microliters of S9 in one ml of S9 mix 

Mixture = Microliters of S9 nix per plate 

Chemical = Code for chemical tested 

Strain = Salmonella strain used 

Plated = Microliters of S9 mix plated for sterility plate 

Technician = Code for the technician who performed the work 

Contract No. = Contract number of project 

Activation = Code number for the hatch of S9 mix 

Blank for testing without S9 

Batch = Code for the strain batch 

Colonies = Number of colonies on S9 sterility plate 

Generally blank if S9 was sterile 

S05^ = Solvent (Dimethylsulfoxide) 

P = Positive controls 

P-01 = Sodium azide 

P-02 = 9-Aminoacridine 

P-03 = 2-Nitrofluorene 

P-04 = 2-Aminoanthracene 

STD DEV = Standard deviation of replicate plate counts 

Q/C = Quality control column 

A = Acceptable, i.e., within the ranges specified for each project 

N/A = Not acceptable 

VR = Viability ratio, the ratio of surviving colonies with sample to 

the surviving colonies of the solvent control 

TOX = Toxicity - an asterisk appears in this column for the lowest dose 

with a viability ratio of 0.5 or greater 

(continued) 

Ji 
P.43 

Legend for Computer Output (completed) 

MR = Mutagenic ratio - the ratio of the number of revertants with 

sample over the number of revertants with the solvent control 

MEC = Minimum effective concentration, the lowest dose with a MR of 

2.0 or greater for TA98 and TAlOO, and 3.0 or greater for 

TA1535, TA1537 or TA1538 

Please note that for the outagenicity data, the positive controls 

and the samples are in units of micrograms/plate, while the solvent 

controls are in units of microliters/plate. V 

35 
TOXICIT Y OF FM - TAlOO 

P.44 

TOXI C 

EXP DATE : 

TEST TYPE: 

DILUTION : 

MIXTURE: 

I T Y 

032883 

05 

CHEMICAL : FM 

STRAIN : TAIOO 

PLATED= 

TECHNICIAN: LGM 

CONTRACT NO.: 191482 

ACTIVATION: 

6ATCH: C273 

COLONIES= 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE A: 

COUNT BG 

PLATE BI 

COUNT PG 

PLATE 

COUNT 

c: 

BG 

AVERAGE 

COUNT 

STD DEV 

COUNT 

VR TO 

Sl 99 0.C0 

1.00 

ICO.00 

250.00 

500.00 

1000.00 

256 

451 

224 

143 

0 

495 

174 

468 

241 

0 

0 

391 

360 

535 

244 

149 

0 

123 

263. 3 

484. 7 

236. 3 

97. 3 

0 . 0 

336. 3 

93.2 

44.4 

10.8 

64.3 

O.C 

191.9 

1.0 

1.8 

0.9 

0.4 

o.o: 

1.3 

TOXICIT Y 

EXP DATE: 032883 

TEST TYPE: 05 

DILUTION: K 20 

MIXTURE: 500 

CHCMICAL : FM 

STRAIN : TAIOO 

PLATED: 1C0 

TECHNICIAN: LGM 

CONTRACT NO.: 191482 

ACTIVATION: CSDC02 

BATCH: C273 

COLONIES: 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE 

COUNT 

A: 

BG 

PLATE B: 

COUNT PG 

PLATE c: 

COUNT BG 

AVERAGE 

COUNT 

STD DEV 

COUNT 

VR TO. 

Sl 99 0.G0 

1.C0 

100.00 

250.00 

500.00 

1300.00 

256 

310 

251 

15C 

21 

0 

174 

350 

275 

109 

9 

0 

36C 

305 

336 

152 

8 

C 

263.3 

321.7 

287.3 

137.0 

12.7 

0.0 

93.2 

24.7 

43.e 

24.3 

7.2 

O.t 

1.0 

1.2 

1.1 

0.5i 

0.0 

0.0 
P.45 

R 

E 

V 

E 

R 

T 

A 

N 

T 

C 

0 

U 

N 

T 

S=SOLVENT PsPOSITIVE N=ANTHRAKINE W/O S9 *:TEST COMPOUND 

C0N:191482 CHEM=FM STRAIN:100 DATE:032883 ACTIV= ACT*:05 

PLOT OF AVGCOUNTOOS E SYMBOL IS VALUE OF SPIND 

i 

i 

550. 0 • 

! 

i 

500. 0 • 

450. 0 • 

400. 3 • 

350. 0 

300. 0 • 

250. 0 • 

200. 0 • 

150. 0 • 

1C0. 0 

5 0 . 0 • 

0 . 0 • 

10 ICC 

DOSE IN MICF.OGPAHS 

i:oo i: JI 

37 
r t 

^ 

v 

r 

F 

T 

A 

N 

T 

C 

0 

U 

N 

T 

UAit-03288 3 ACTIV=CSD00 2 ACT*=O E 

i 

i 

330. 0 • 

; 

t 

i 

300. 0 • 

270. 0 • 

240. 0 • 

210. 0 • 

180. 0 • 

150. 0 • 

120. 0 

90. 0 • 

60. 0 • 

30.C 

0.G 

PLO T OF AVGC0UNT«DOS E 

SYMBO L IS VALU E OF SPIN D 

10 IOC 10C 0 1 PC i 

DOS E IN MICROGRAM S 
P.47 

# TOXICITY OF TRC - TAlOO 

TOXI C 

EXP DATE : 

TEST TYPE 

OILUTION : 

MIXTURE : 

SOLVENT/ 

POSITIVE 

Sl 99 

i 

I T Y 

032683 

: 05 

• • 

DOSE 

LEVEL 

0.00 

100.00 

333.00 

000.00 

33d3.G0 

10000.00 

CHEMICAL : TRC 

STRAIN 

PLATED: 

: TAlOO 

-TECHNICIAN: LGM 

PLATE A: 

COUNT BG 

256 

410 

410 

151 

lb 

17 

PLAT E B : 

COUNT PG 

174 

332 

367 

203 

0 

52 

CONTRACT NO. : 

ACTIVATION : 

BATCH : C273 

COLONIES: 

PLATE C: 

COUNT BG 

360 

3C2 

446 

221 

0 

22 

AVERAGE 

COUNT 

263.3 

348.0 

407.7 

191.7 

5.3 

30.3 

•1914fc2 

STD DEV 

COUNT 

93.2 

55.7 

39.6 

36.4 

9.2 

18.9 

: 

, 

! 

i 

VR 

1.0 

1.3 

1.5 

0.7 

0.0 

0.1 

TO 

TOXICIT Y 

EXF OATE: 032883 

TEST TYPE: 05 

DILUTION: l: 20 

MIXTURE= 500 

CHEMICAL : TRC 

STRAIN : TAlOO 

PLATED : 120 

TECHNICIAN : L'GM 

CONTRACT NO. : 191482 

ACTIVATION : CSD0C2 

BATCH : C273 

COLONIES : 

SOLVENT/ 

POSITIVE 

COSE 

LEVEL 

PLATE 

COUNT 

A: 

BG 

PLATE B : 

CCUNT ?G 

PLATE 

COUNT 

c: 

PG 

AVERAGE 

COUNT 

STD DEV 

COUNT 

VR TC) 

-1 99 C O O 

ICC.CO 

333.00 

100C.C0 

3333.CO 

KOOC.CO 

256 

119 

429 

51 

0 

0 

174 

365 

436 

187 

0 

0 

36C 

410 

363 

46 

c 

0 

263.3 

29F.C 

416. C 

94.7 

0.0 

0.0 

93.2 

156.^ 

2*.8 

80.0 

0.0 

0.0 

1.0 

1.1 

1.6 

0.4 

0.0 

o.e 
' I' ! 

P.48 

S=SOLVENT P=POSITIVE N=ANTHRAMINE W/O S9 »:TEST COMPOUND j 

C0N=191482 , CHEM:TRC STRAIN:100 DATE=0328& 3 ACTIV : ACTX=05] 

PLOT OF AVGCCUNToDOSE SYMBOL IS VALUE OF SPIND j 

R 

E 

V 

r 

R 

T 

A 

fj 

T 

C 

0 

u 

u 

T 

450.0 • 

400.0 • 

! 

350.0 • 

300.0 • 

250.C • 

200.C • 

150.0 • 

100.C • 

50.0 • 

0.0 • 

10 

. - 4 -< 

IOC 1PC0 

. - yyy . 

1 00 c 

DOSE IN MICROGRAM S 
S=S0LVEN T P:PCSITIV E N:ANTHRAMIN E W/O S9 *:TEST COMPOUN D 

C0N:19148 2 CHEM:TR C STRAIN=10 0 DATE:03286 3 ACTIV:CSD0C 2 ACT*:05 

PLOT OF AVGCOUNT«DOS E SYMBOL IS VALUE Or SPIN D 

P.49 

R 

E 

V 

E 

R 

T 

A 

N 

T 

C 

0 

u 

N 

T 

450. 0 • 

400. 0 • 

350. 0 • 

300. 0 

250. C • 

200. 0 «• 

150. 0 • 

100. 0 • 

50. 0 • 

O.C • 

10 IOC 

DOSE IN MICROGRAM S 

10C0 COO! 

41 
TOXICIT Y OF ECC - TAlOO 

P.50 

TOXI C 

EXP DATE: 

TEST TYPE 

DILUTION: 

MIXTURE= 

I T Y 

032883 

05 

CHEMICAL: EDC 

STRAIN: TAlOO 

PLATED: 

TECHNICIAN: LGM 

CONTRACT NO.: 

ACTIVATION: 

BATCH: C273 

COLONIES: 

191482 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE 

COUNT 

A: 

BG 

PLATE e: 

COUNT BG 

PLATE C: 

COUNT PG 

AVERAGE 

COUNT 

STO OEV 

COUNT 

VR TOX 

Sl 99 0.00 

50.CO 

100.00 

250.00 

500.00 

1000.00 

256 

483 

467 

347 

388 

327 

174 

484 

530 

154 

342 

269 

360 

427 

375 

364 

404 

34 3 

263. 3 

464. 7 

457. 3 

288. 3 

378. 0 

313. 0 

9 J. 2 

32.6 

78.0 

116.6 

32.2 

38.9 

1.0 

1.8 

1.7 

1.1 

1.4 

1.2 

TOXICIT Y 

EXP DATE: 032683 

TEST TYPE: 05 

DILUTION: l: 20 

MIXTURE: 500 

CHEMICAL : EDC 

STRAIN : TAIO C 

PLATED = IC O 

TECHNICIAN : LGM 

CONTRACT NO.: 191482 

ACTIVATION: CSD002 

BATCH: C273 

COLONIES: 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE 

COUNT 

A: 

PG 

PLATE 

COUNT 

B : 

BG 

PLATE 

COUNT 

c: 

PG 

AVERAGE 

COUNT 

STD DEV 

COUNT 

VR TOX 

Sl 99 o.co 

5C.00 

100.CO 

250.00 

500.00 

1300.00 

256 

410 

424 

440 

506 

308 

174 

428 

462 

365 

442 

234 

360 

457 

392 

567 

432 

263. 3 

375. 3 

447. 7 

399. 0 

505. 0 

324. 7 

9J. 2 

76. 2 

20. 6 

38. 0 

62. 5 

100 . c 

1.0 

1.4 

1.7 

1.5 

1.9 

1.2 

42 
ff 

P.51 " | • i 

S:S0LVENT P:P0SITIVE N:ANTHRAMINE W/O S9 *:TEST COMPOUND 

C0N:191482 CHEM=EDC STRAIs:100 DATE=0328e3 ACTIV: ACT«:Q5 

PLOT OF AVGCOUNT«DOSE SYMBOL IS VALUE OF SPIND 

R 

E 

V 

r 

R 

T 

A 

N 

T 

C 

0 

u 

r, 

T 

I 

I 

550.0 • 

! 

• 

500.0 • 

! 

i 

i 

450.0 • 

400.0 • 

350.0 • 

300.0 • 

250.0 • 

200.0 • 

150.0 • 

.10 0.0 • 

50.0 • 

0.0 • 

10 IPO 1300 1 CCC 

DOSE IN MICROGRAMS 

43 
S=SOLVEN T P:POSITIVE N:ANTHRAMINE W/O S9 *=TEST COMPOUN D 

C0N=191482 CHEM=EDC STRAIN=100 DATE:03288 3 ACTIV:CSD00 2 ACT*:05 

PLOT OF AVGCOUNT*DOSE SYMBOL IS VALUE OF SPIND ! 

P 

r 

V 

r 

R 

T 

A 

N 

T 

C 

0 

u 

N 

T 

55fr»« • 

500. 0 • 

450. 0 • 

400. 0 • 

350. 0 • 

i 

i 

! 

3CC.0 • 

250..0 • 

200. 0 • 

150. 0 • 

100. C • 

50. 0 • 

0 . 0 • 

10 IOC 

DOSE IN MICROGRAMS 

1000 II 3000 

44 
TOXICIT Y OF E C - TAlO O 

P.53 

TOXI C 

EXP DATE: 

TEST TYPE 

DILUTION^ 

MIXTURE= 

SOLVENT/ 

POSITIVE 

Sl 99 

I T Y 

032883 

: 05 

• • 

CHEMICAL: EC 

STRAIN: 

PLATED= 

TAlOO 

TECHNICIAN: LGM 

DOSE PLATE A: 

LEVEL CC 

0.00 

100.00 

333.00 

1000.00 

3333.00 

10000*00 

TOXI C 

EXP DATE: 

TEST TYPE 

DILUTION: 

MIXTURE: 

I T Y 

032883 

: cs 

I: 20 

500 

)UNT BG 

256 

267 

375 

369 

425 

80 

PLATE B: 

COUNT BG 

174 

554 

532 

3*2 

537 

124 

CHEMICAL: 

STRAIN: 

PLATED: 

TAlOO 

ICO 

TECHNICIAN: LGM 

CONTRACT NO.: 

ACTIVATION: 

BATCH: C273 

COLONIES: 

PLATE C: 

COUNT BG 

360 

433 

523 

502 

599 

167 

AVERAGE 

COUNT 

263.3 

41B.0 

476.7 

404.3 

520.3 

123.7 

CONTRACT NO.: 

191*82 

STD DEV 

COUNT 

93.2 

144.1 

88.2 

85.7 

88.2 

43.5 

191482 

ACTIVATION: CSD002 

BATCH: C273 

COLONIES: 

VR 

1.0 

1.6 

1.8 

1.5 

2.0 

0.5 

TCX 

SOLVENT / DOS E PLAT E A : PLAT E B : 

POSITIV E LEVE L COUN T BG COUN T B G 

Sl 0.00 

100.00 

333.00 

1000.00 

3333.00 

10000.00 

256 

463 

391 

624 

683 

0 

174 

533 

304 

438 

745 

0 

PLATE 

COUNT 

360 

405 

337 

450 

624 

0 

c: 

BG 

AVERAGE 

COUNT 

263.3 

467.0 

3«4.0 

504.0 

6c4.0 

0.0 

STD DEV 

COUNT 

93.2 

64.1 

43.9 

104.1 

60.5 

0.0 

VR 

1.0 

I.P 

1.3 

1.° 

2.6 

O.C 

TOX 

A 5 
P.54 

S = SOLVENT P:P0SITIV E N = ANTHRAMINE U/O S9 * = TE ST COMPOUND 

C0N:19148 2 CHEM:EC STRAIN:10 0 DATE:Q3288 3 ACTIV= ACTX=05 

PLOT OF AVGCOUNT«OOSE SYMFOL I S VALUE OF SPIN D 

i 

I 

550. 0 • 

! 

| 

! 

500. 0 • 

» 

! 

<»50.0 • 

400. 0 • 

! 

! 

i 

350. 0 • 

! 

300. 0 

250. 0 

200. 0 

150 . C 

100. 0 

50. 0 

o . e • 

10 u o 

DOS E IN MICROGRAM S 

IC'JW 10003 

46 
P.55 

S:SOLVENT P:P0SITIVE N:ANTHRAMINE W/O S9 «:TEST COMPOUND 

C0N:191482 CHEM:EC STRAIN=100 DATE=032883 ACTIV=CSD0C2 ACTX=C5 

PLOT OF AVGCOUNT*DOSE SYMBOL IS VALUE OF SPIND 

R 

r 

V 

E 

R 

T 

A 

N 

T 

C 

0 

U 

N 

T 

900. 0 • 

! 

i 

i 

! 

800. 0 • 

700. 0 • 

600. 0 • 

500. 0 • 

400. 0 • 

300. 0 • 

200. 0 

1CO. O • 

0 . 0 • 

10 100 

DCSE IN MICROGRAMS 

13C0 1 CO OC 

47 
P.56 

r- TOXICITY OF NP - TA10C 

TOXI C 

EXP DATE: 

TEST TYPE 

DILUTION: 

MIXTURE: 

I T Y 

032883 

05 

• 

CHEMICAL: NP 

STRAIN: TAIOO 

PLATED: 

TECHNICIAN: LGM 

CONTRACT NO, 

ACTIVATION: 

BATCH : C273 

COLONIES: 

1914E2 

SOLVENT/ DOSE PLATE A: PLATE B: PLATE C: AVERAGE STD DEV 

POSITIVE LEVEL COUNT BG COUNT BG COUNT BG COUNT COUNT 

Sl 99 0.00 

100.00 

333.00 

1000.00 

3333.00 

10000.00 

256 

478 

479 

365 

190 

277 

174 

431 

387 

460 

234 

225 

360 

396 

305 

283 

326 

245 

263. 3 

435. 0 

390. 3 

369. 3 

250. 0 

249. 0 

93.2 

41.1 

87.0 

68.6 

69.4 

26.2 

VR 

1.0 

1.7 

l.S 

1.4 

0.9 

0.9 

TOX 

TOXICIT Y 

EXP DATE: 032883 

TEST TYPE: 05 

DILUTION: l: 20 

MIXTURE: SOO 

CHEMICAL: NP 

STRAIN : TAlOO 

PLATEC: 100 

TECHNICIAN: LGM 

CONTRACT NO.: 191482 

ACTIVATION: CSD002 

BATCH: C273 

COLONIES: 

SOLVENT/ 

POSITIVE 

S l 99 

DOSE 

LEVEL 

COO 

IOC.00 

333.3 0 

1000.0 0 

3333.0 0 

10000.0 0 

PLATE A! 

COUNT BC 

256 

491 

393 

401 

462 

62 

PLATE e: 

COUNT BG 

174 

309 

475 

334 

396 

75 

PLATE 

COUNT 

360 

393 

508 

445 

356 

76 

c: 

FG 

AVERAGE 

COUNT 

263.3 

396.7 

458.7 

3 = 3.3 

404.7 

77.7 

STD DEV 

CCUNT 

9».2 

91.2 

59.2 

55.9 

53.5 

3.8 

VR 

I.C 

1.7 

1.5 

1.5 

0.3 

TOX 
P.57 

S:SOLVCNT P:POSITlVE K:&NTHR4MINE U/O S9 .:TEST CC-POUND 

C0N:lt?1482 CHEM:NP STRAIN:100 CATE=03288 3 ACTIV= ACTx=0S 

PLOT OF AVGCOUNT*DOSE SYMBOL IS VALUE OF SPIND 

450. C • 

| 

! 

I 

400. 0 

350. 0 

300. 0 

250. 0 

200. 0 

isc: * 

1 0 0 

50. 0 

o.e 

. . . • • • • 

1 0 IC C 

CCSE IN MICROGRAM S 

ice o 

« 

1 cc c 

•49 
P.58 

S:SOLVENT P:PCSITIVE N=ANTHRAMINE U/O S9 *:TEST COMPOUND 

C0N:19l4-82 CHEM:NP STRAIN=100 DATE:032683 ACTIV:CSD00 2 ACT*:Q5 

300. 0 

250 . C 

200 . C 

150. C 

100. C • 

50. 0 • 

! 

PLOT OF AVGCOUNT«DOSE SYMPOL IS VALUE OF SRIND 

450. 0 • 

! 

! 

! 

| 

400. 0 • 

! 

35C.C 

* 

o.e 

10 IOC 

DOS E IN MICROGRAM S 

100 3 1 CC CC 

50 
P.59 

! 

TOXICITY OF NB - TAlOO 

TOXI C 

EXP DATE : 

TEST TYPE 

DILUTION: 

MIXTURE: 

I T Y 

032683 

05 

• • 

CHEMICAL : NB 

STRAIN : TAlOO 

PLATEC: 

TECHNICIAN: LGM 

SOLVENT/ DOSE PLATE AI PLATE B: 

POSITIVE LEVEL COUNT BG COUNT BG 

CONTRAC T NO. : 19148 2 

ACTIVATION : 

BATCH : C273 

COLONIES: 

Sl 99 0.00 

100.CO 

333.00 

1000.00 

3333.00 

10000.00 

T O X I C I T Y 

EXP DATE: 032883 

TEST TYPE: 05 

DILUTION: i: 20 

MIXTURE: 500 

256 

257 

367 

151 

179 

174 

243 

302 

218 

230 

PLATE C: 

COUNT BG 

360 

0 

0 

17b 

154 

AVERAGE 

COUNT 

263. 3 

166. 7 

223. 0 

181. 7 

187. 7 

STD DEV 

COUNT 

93.2 

144.5 

195.6 

VR 

1.0 

0.6 

o.e 

33.9 

36.7 

CHEMICAL : NB 

STRAIN : TA1C0 

PLATED: 100 

TECHNICIAN: LGM 

CONTRACT NO.: 191462 

ACTIVATION : CSD002 

BATCH : C273 

COLONIES: 

SOLVENT/ DOSE PLATE A! 

POSITIVE LEVEL COUNT BG COUNT BG 

Sl 99 0. CO 

100.CO 

333.00 

1000.00 

3333.00 

10COD.OC 

256 

392 

«70 

339 

194 

302 

ATE B : 

UNT BG 

174 

379 

300 

184 

6 

13 

PLATE 

COUNT 

360 

431 

355 

166 

273 

*6 

c: 

BG 

AVERAGE 

COUNT 

263.3 

400.7 

375.0 

229.7 

157.7 

133.7 

0.7 

0.7 

STD DFV 

COUNT 

93.2 

27.1 

86.7 

95.1 

137.2 

161.2 

VR 

1 

1 

1 

0 

0 

0 

• 3 

.5 

.0 

.9 

.6 

.5 

TOX 

TOX 

51 
P.60 

* t : 

R 

E 

V 

E 

P 

T 

A 

\ 

T 

C 

0 

u 

N 

T 

S:SOLVENT F:P0SITIVE N:ANTHRAMINE W/O S9 »:TEST COMPOUND 

C0N:191482 CHEM:NE STRAIN:1C0 OATE:032883 ACTIV: ACT* :05 

; 

t 

275. 0 • 

i 

! S 

250. 0 • 

! 

! 

• 

225. 0 • 

! 

t 

; 

200. 0 • 

; 

i 

• 

175. 0 • 

t 

150. 0 • 

i 

i 

i 

125. 0 • 

i 

i 

• 

100. 0 • 

J 

• 

I 

75. 0 • 

i 

I 

i 

50. 0 • 

• 

i 

i 

25. 0 • 

i 

; 

i 

C O • 

PLOT OF AVGCOUNT»DOSE SYMBOL IS VALUE OF SPIND 

NCTE: 

0 1 10 IOC 

OOS E IN MICPOGRA" S 

1 OP S HA D MISSIN G VALUE S OK WE P E OUT CF RfcN'GE 

1 CC 0 

. - -- 4 . 

i :cot 

52 
# 

P.61 

S=SOLVENT P:P0SITIV E N:ANTHRAMJNE W/O S9 «:TES T COMPOUND 

C0N:19148 2 CHEM=NB STRAIN:1C 0 DATE=03288 3 ACTIV:CSD00 2 ACTX:0 b 

PLOT OF AVGCOUNT*DOSE SYMEOL I S VALUE OF SPINO 

R 

P 

V 

E 

R 

T 

A 

r. 

T 

c 

O 

u 

N 

T 

450. 0 • 

i 

i 

f 

j 

400. 0 • 

350. 0 • 

300. 0 • 

250. 3 

200. 3 • 

150 . C 

100 . C 

50. 0 • 

C O • 

— • . 

1 0 130 10C 0 i :• o c c 

DOSE I N MICROGRAMS 

53 
MUTAGENICIT Y OF MC - TA9 7 

P.62 

MUTAGENICIT Y 

EXP PATE: 031683 CHE M ICAL : MC 

TEST TYPE: C5 STRAIN: TA97 

DILUTION: : PLATED: 

MIXTURE= TECHNICIAN: LGM 

CONTRAC T NO. : 

ACTIVATION : 

PATCH : C173 

COLONIES: 

191482 

SOLVENT/ 

POSITIVE 

COSE 

LEVEL 

PLATE A: 

COUNT BG 

PLAT E e: 

COUNT PG 

FLATE 

COUNT 

c: 

P.G 

AVERAG E 

COUN T 

S T D DE V 

COUNT 

G/C MR MEC 

Sl 

PI 

P2 

99 

02 

02 

0.C0 

5C.C0 

10CP0 

1300.00 

2500.CO 

5!?00.C0 

75C0.CC 

i c : c c o o 

163 

4T3 

1241 

12° 

151 

153 

177 

11° 

144 

15? 

1123 

145 

162 

13? 

125 

:3e 

160 

517 

829 

173 

147 

153 

129 

132 

155.7 

426.C 

1364.3 

150.7 

153.3 

145.0 

143.7 

129.7 

10.? 

83.8 

212.2 

25.3 

7.8 

11.4 

28.9 

9.7 

A 

A 

A 

I.C 

2.7 

6.8 

1.0 

1.3 

0.9 

C 9 

CF. 

MUTAGENICIT Y 

EXP TATE: C31PF.3 CHEMICAL : MC 

TEST TYPE: C5 STRAIN: T497 

DILUTION: l: 20

 CLATC:: 130 

MIXTURE: 5C0 TECHNICIAN: LGM 

CONTRACT NO.: 1914B2 

ACTIV ATIO'«: C SDC 32 

BATCH: C173 

COLONTES= 

SOLVENT/ 

POSITIVE 

COSE 

LEVEL 

FLATT 

CCU'.T 

A: 

BG 

PLATE F.: 

C?'UNT BG 

FLATE 

COUNT 

c: 

EG 

AV^FAGE 

COUNT 

STD DFV 

COUNT 

C/C MR MEC 

Sl 99 

PI 34 

F2 34 

O.OC 

1. re 

2.CO 

1 / O w • W I 

2

c . : c. : c 

5 ? c c . c 0 

7 ?• :• 3 . c 0 

. W .. L. v. • y O 

IB? 

1 CIO 

92C 

171 

17B 

226 

217 

233 

175 

1437 

9 33 

138 

194 

.223 

??3 

220 

2C2 

1401 

913 

176 

1BF 

22c 

2 if. 

1.-6.3 

1272. 7 

922.C 

161. 7 

1H6.7 

217.C 

220. 0 

2 2 2. 0 

14. 3 

227. 5 

I C I 

20. 6 

B .l 

1 0 . P. 

3 . 0 

7 . ? 

1.3 

6 . 8 

4 . 9 

0 . 9 

I.C 

1.2 

1.2 

1.2 

M U T A 0 E N I C I T Y 

EXP DiTE : 

TEST TYFE 

DILUTION : 1 

MIXTURE: f>0 1 

3 3 1 r. 8 3 

C5 

CHEMICAL : MC 

'••TRAIN: ~ A 9 7 

PLATF.:= 10 0 

TECH?'ICIiN= Lf-M 

CONTRACT NO.: 1914&2 

ACTIVATION : HEPA31 

B t T C H : C 1 7 3 

COLOME S = 

SGLVTM / 

POSITIV E 

DOS E 

LEVE L 

F L A T r A : 

COUNT EG 

PL'.TE *: 

CO'JNT BG 

FLATL 

CCUNT 

c: 

PC 

A V E F A I- E 

CCUN T 

S TD D:V 

COUNT 

C/C MR r:c 

£1 

PI 

r2 

99 

C4 

04 

C C O 

c.r.c 

i.:o 

1 COO.3 0 

2 5 0 C C 0 

5CCC3 0 

7 5 0 C 3 0 

1C30CC 0 

227 

<?:?. 

lbC21° 

2?* 

194 

2CS 

177 

232 

1 r- 01 

1 3 56 

2 44 

r o 5 

?20 

?44 

197 

162 

9-4 

1454 

19C 

215 

233 

245 

201 

1-7.0 

94 4.3 

1436.3 

217.7 

215.3 

21c-.7 

2 31.3 

191.7 

3?.* 

4C.7 

75.7 

27.C 

1C.5 

19.9 

22.° 

12.9 

bh 
P.63 

-•wrr-"*" * -*«»fltfr*^r *•: i*»w -i,-».>»-*ii 

S:SOLVEMT P:FOSlTIV E N':ANTHRAMINE W/O S9 *:TES T COMPOUND 

CON:10148 2 CHEM:MC STRAIN:9 7 DATE=0318e 3 ACTIV : ACTt:0 5 

PLOT OF AVGC0UNT«D0SE SYMBOL I S VALUE OF SPIN D 

1100. 0 • 

1000. 0 • 

90C C • 

R 

E 

V 

E 

-X 

T 

A 

N 

T 

C 

O 

u 

N 

T 

soo. c 

700. 0 

600. 3 

500. 0 

400. 0 

33C.3 

• 

• 

• 

• 

• 

• 

200. C • 

ioo. e 

o . e • 

• ••-»• » — » — — •»••"•* * TM" 

10 ic o 

DOSE I N MICROGRAMS 

. . * * 

1C3 0 1 30 0 C 

55 
S=SOLVENT P:PCSIT!VE N:ANTHRAU 1 NE W/O S9 *=TEST COMPOUND 

C0N:191462 CHEM=MC ST»AIN=97 DATE:031883 ACTIV:CSD002 ACT*: 05 

PLOT OF AVC-COUNT*OOSE SYMPOL IS VALUE OF SPIND 

R 

E 

V 

E 

R 

T 

A 

N 

T 

C 

0 

u 

N 

T 

15C0. 0 

1400. 0 

1300. C 

12C0.C 

1100. 0 

1500. 0 

900. 0 

EOD.O 

700. 0 

6 0 0 . C 

500. 3 

4 C 0 . 0 

3C0.G 

230. 0 

100 . C 

C C 

1 

1 

J 

t 

• 

1 

• 

• 

1 

1 

1 

1 

• 

t 

1 

• 

1 

1 

• 

I 

• 

4 

1 

. 1 

• 

1 

1 

1 

t 

1 

1 

1 

1 

1 

1 

1 

f 

• 

10 ic; ICC O 1 3 : o o 

ECSE IN MICROGRAMS 

56 
P.65 

S:SOLVEN T P:F0SIT!VE N=ANTHRAHINE U/O S9 «:TEST COMPCUNO 

CC\:l=14e 2 CHt**sHC ST=AIN=97 DATE:031863 ACTIV:HEP*01 ACTX=OS 

PLOT OF AVT-COUNT.DOSE SYMBOL IS VALUE OF SPIND 

1500. C 

1400. 0 

1300. 0 

1203. C 

11 CCC 

1C0C.C 

v 00 . C 

eoo. : 

7 3 C . C 

60G.C 

5C0.C 

40C. 3 

33C. 0 

200. C 

100. 0 

0 . 0 

« 

. * 

IC ICC 

HOSE IN KICROGRAK5 

l r r. D 1 P 3 Q 0 

57 
P.66 

MUTAGENICITY OF MC TA98 

H U T A G 

EXP DATE : 

TEST TYPE : 

DI LOT I ON : 

MIXTURE : 

E N I C 

031683 

05 

T Y 

CHEMICAL : 

STRAIN : TA 

PLATED : 

TECHNICIA N 

MC 

98 

LG« 

CONTRAC T NO.1 

ACTIVATION : 

BATCH : C173 

COLONIES : 

191482 

SOLVENT / 

POSITIVE 

DOSE 

LEVEL 

PLATE 

CCUNT 

A : 

BG 

PLATE E: 

COUNT BG 

PLATE C: 

COUNT BG 

AVERAGE 

COUNT 

STD DEV 

COUNT 

O/C MR MEC 

Sl 

»1 

P2 

99 

03 

03 

0.00 

1.30 

2.00 

1330.00 

2500.CO 

5?C0.00 

7500.00 

1C00C30 

27 

23? 

259 

5? 

53 

47 

4? 

54 

26 

160 

?89 

55 

74 

70 

61 

59 

22 

172 

344 

59 

57 

56 

59 

56 

25.0 

159.0 

297.3 

55.7 

61.3 

57.7 

56.3 

56.3 

2.6 

40.3 

43.1 

3.1 

11.2 

11.6 

6.4 

2.5 

A 

A 

A 

1.0 

7.6 

11.9 

2.2 

2.5 

2.3 

2.3 

2.3 

M U T A E N I C I T Y 

EXP DATE: C31683 

TEST TYP": 05 

DILUTION: K 2 0 

MIXTURE= 500 

CHEMICAL : MC 

STRAIN : TA98 

^LATED: 109 

TECHNICIAN: LGM 

CONTRACT NO-.: 191462 

ACTIVATION''': CSDCC2 

BATCH : C173 

COLONIES: 

SOLVENT/ 

POSITIVE 

DorLEVL L 

PLAT E 

COUN T 

A : 

EG 

PLATE P ; 

COUNT PG 

"LATE 

COUNT 

c: 

PG 

AVERAGE 

COUNT 

S TD CEV 

COUNT 

O/C MR MEC 

Sl 

PI 

P2 

99 

34 

04 

0.0 0 

1.0 0 

2.30 

15:3.30 

2 5 3 3. C 0 

5 3 3 C C 0 

75CCC 0 

13 3 3C3 C 

53 

16CS 

1663 

73 

= 4 

45 

R4 

f 7 

31 

1358 

1663 

64 

92 

14 

94 

87 

39 

1559 

1634 

96 

73 

24 

88 

t8 

41. 0 

1538. 3 

1653. 3 

84. 0 

86. 3 

27. 7 

Re.7 

P C 7 

11. 1 

132. 5 

16. 7 

1 2 . C 

11. 6 

15. 8 

5 . 0 

11. F 

NA 

A 

A 

I . C 

36. e 

40. 3 

2 . 0 

2 . 1 

0 . 7 

2 . 2 

2 . 0 

M U T A G 

E XF DATE : 

TES T TYPE: 

DILUTION : 

MIXTU'rE = '. 

Z U I C I T Y 

031683 CHEMICAL : MC 

3

C. STRAIN : Tigs 

l: 20 PLATED : 130 

OC TECHNICIAN : LC-M 

CONTRACT NO. : 1914P.2 

ACTIVATION': HEPAC 1 

BATCH : C173 

COL C M FS = 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE 

COUNT 

A : 

BG 

PLATE B". 

COUNT

 B G 

PLATE C: 

COUNT ECAVERAGE 

COUNT 

STC CEV 

CCUNT 

O/C MR MEC 

Sl 

PI 

F2 

99 

04 

C O O 

0. 5C 

1.0C 

icoo.co 

2530.00 

5C0CC 0 

7500.CO 

1 C 3 3 0 . C C 

3C 

1252 

1673 

i,-. 

i • c 

94 

114 

42 

1634 

2131 

60 

63 

107 

77 

Rl 

2.° 

1513 

2 10 = 

56 

61 

70 

R5 

p a 

33. 3 

1466. 3 

2 P 3 3 . 3 

62. 3 

76. 7 

82. 0 

R5.3 

97. 7 

7 . 6 

195. 2 

121 .=> 

5 . ? 

9 . 3 

21. 7 

8 . 5 

16. 5 

1.3 

44.C 

b C Z 

1 . 9 

2 . 3 

2.E 

2 . 6 

2 . c 
R 

E 

V 

E 

R 

T 

A 

N 

T 

C 

0 

u 

N 

T 

S::OLVEN T P:PCSITIV E N = ANTHRA«INE W/O S9 »:TES T COMPOUND 

C0V:1914e 2 CHEM = MC STRAIN:9 8 DATE:03186- 3 ACTIV : ACTX:0 5 

PLOT OF AVGCOUNT.DOSE SYMBOL I S VALUE OF SPIN D 

330. 0 • 

300. 0 • 

27C. t • 

240. C • 

210. 3 • 

leCC i 

150.' . • 

123. 3 • 

9 3 . C 

6 0.' . 

30. 3 

0 . 3 • 

. * 

m 13 0 

: r S E IN "ICRTGPAMS 

1 OC 0 1 3C3 3 

y ? 
P.68 

S-SOLVEN T P = PCSITIV E N = ANTHF. AM IN E W/ O S 9 «=TES T COMPOUN D 

: 0N = 19146 2 CHE M = M C STJ.AI N = 9 8 0AT E = C3186 3 ACTIV=CSD,) 2 AC U = 0' 

PLO T O F AVGCOUNT.DOS E SYMBO L IS VALU E OF SPIN D 

R 

E 

V 

E 

R 

T 

A 

N 

T 

C 

0 

u 

N 

T 

leoo.o • 

1600.0 • 

1400. . • 

12CC0 • 

10CC3 • 

603, 

6 C 3 . r • 

400 . 

2 0 0 . C • 

C O • • - • • 

10 

. - • - . 

10 j 

.•- + -. 

1CC C 

, . - - 4 • 

1 0 C 3 C 

; 0 S E IN MICROGRAM S 

60 
,J..,^y. i-.r-y 

CON:!^*62 CHE' 

PLOT OF AV GCOUNf*DOSE 

u / 0 co .--TEST COMPOUND 

THR *M l VlBb 3 ACT1V=HEPA01 

SYMBOL IS ,.»«.r-sK "•"'"-'-'"" « v l - -» 

I 

2250. 0 • 

200C. 0 • 

1750. 3 

R 

E 

V 

E 

R 

T 

A 

N 

T 

1503 -

C 

0 

u 

N 

T 

• 

i 

i 

i 

i 

• 

« 

1250. T 

ico c o 

I 

I 

* 

1 

1 

I 

I 

75G.0 

500. 0 

250. 0 • 

i S 

0 . 0 * 

• «•• " 

0 

10 

DCSE 

I C O 

T N KlCR r

-

G P ^$ 

i: co 

13 3 0 3 

61 
MUTAGENICITY OF "C - TA1C0 

MUTAGENICIT Y 

EXP DATE: 031683 

TEST TYPE: 05 

DILUTION: : 

MIXTURE: 

CHEMICAL: MC 

.STRAIN : TAlOO 

PLATE?: 

TECHNICIAN: LGM 

CONT°ACT NO , 

ACTIVATION : 

RATTH : C173 

COLONIES: 

191462 

SOLVENT/ DOSE 

POSITIVE LEVEL 

Sl 99 

PI 01 

P2 Cl 

O.OC 

1.3C 

2.30 

1000.CO 

250CC 0 

5300.00 

7*90.00 

lvwDtO O 

PLATE 

COUNT 

12R 

794 

7C3 

157 

152 

153 

131 

151 

A: 

FG 

PLATE 

C3UNT 

178 

724 

399 

125 

130 

145 

119 

150 

c > : 

9G 

PLATE 

COUNT 

163 

544 

601 

137 

198 

11° 

123 

163 

c: 

PG 

AVERAGE 

COUNT 

156.3 

687.3 

567.7 

139.7 

130.3 

139.0 

124.3 

154.7 

STD DEV 

COUNT 

25.7 

129.0 

154.7 

16.2 

2 2. C 

17.8 

6.1 

7.2 

O/C 

A 

A 

A 

MR 

1.0 

4.4 

3.6 

0.9 

O.P 

0.° 

O.P 

1.0 

MEC 

M U T A G N I C I T Y 

EX«> DATE: 031S83 

TEST TYPr: o* 

DILUTION: K 2 0 

MIXTURE: =-3 0 

CHEMICAL: MC 

STRAIN: Tiioo 

PLATED: ICO 

TECHNICIAN: LGM 

CGNTFACT NO.: 191482 

ACTIVATION: CSDC02 

BATCH : ci73 

COLONIES: 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLAT T t: 

CCUNT FG 

F L t T E F : 

C?U\ T ^G 

PLA T 

CCUNT 

c: 

FG 

AVERAGE 

CCUNT 

STD DEV 

CCUNT 

C/C MR MEC 

Sl 

PI 

T2 

99 

04 

C C O 

1.3C 

2.00 

1303.30 

?503.C0 

5:33.30 

7=03.50 

1 3 3 3 C 5 0 

156 

1377 

76<. 

l

c f 

24 * 

153 

U 7 

15: 

166 

l.?26 

72? 

1 99 

16C 

164 

174 

149 

149 

12*3 

633 

1P2 

1*7 

156 

175 

179 

157. 0 

1194. 3 

730. 3 

1*9. 0 

1°7. 3 

156. 7 

172. 0 

1 fc 0 . C 

8.5 

1C5.1 

5 2. C 

8.° 

5 0. C 

7.0 

4.4 

16.5 

1.0 

7.6 

4.7 

1.2 

1.2 

1.3 

1.1 

1.0 

M U T A I C I T Y 

EXP TATE: 331:63 

TEST TYFE*. f5 

DILUTION: I! 2C 

M|XTUi-E= 533 

C«E"ICAL :

 M C 

STFAIN : Til33 

PLATE" : 1 TO 

TECHNICIAN : LG ! 

CCNTPAC T NO. : 1914e ? 

ACTIVATION : HEP£C 1 

c ATCH : en 3 

COLONIES: 

SOLVENT/ 

POSITI v.; LEVEL 

PLAT : 

CCUNT 

t: 

PG 

PL'T E •?: 

CCUN T

 r-G 

PL ATF 

CCUN T 

AVERAG E 

COUN T 

S TD DEV 

CCUNT 

Q/y MR "EC 

Sl ° 9 

PI C4 

F2 34 

c r o 

C 5 0 

1.30 

1 C 3 C 3 3 

2 5 3 3 . C 3 

5 .i 0 C 3 C 

T: :•:..;3 

133C 1 CO 

133 

t--> 

1 3 ^ <• 

l

c

e 

1 Z. ' k -

1 = 5 

1 4 c 

16 = 

136 

56? 

1*4 1 

180 

1 ?5 

19C 

167 

16° 

134 

<t B it 

173 3 

167 

193 

233 

l*3 

19C 

13 4.3 

549.0 

1536.0 

17 6.3 

1 •:. ? . 3 

1 = 5.0 

16 3.7 

162.7 

1.5 

57.7 

199.5 

10.6 

4.3 

5.0 

21 .4 

11.6 

1 

4 

11 

1 

1 

1 

1 

1 

62 
P.71 

r 

S:SOLVENT P:PCSITIVE \: ANT HP A" I NE W/O S<= « = TEST COMPOUND 

C0N:1914e2 CHEM:MC STRAIN=1C0 DATE:C31883 ACTIV: ACT*:C5 

PLOT OF AVCCOUNT'DCSE SYM60L IS VALUE OF SPIND 

R 

E 

V 

E 

R 

T 

A 

N 

T 

C 

n 

u 

N 

T 

9 00. C • 

800. 0 • 

700. 3 •• 

630. 0 * 

500 . 

400. 3 

303. 0 

200 . 0 

1 0 C . 0 

CC • 

1 0 13 3 

;CSE IN »ICF.OGRAuS 

ir: o I c c. c ( 

63 
P.72 

S:SOLVENT P:POSITIV E N:ANT HP AMINE W/O S9 «:TEST COMPOUN D 

C0N:1914P 2 CHEM=MC STRAIN=10 0 OATC:031PS 3 ACT1V:CSD00 2 ACTX:05 

PLOT OF AVGCOUNT*DOS E SYM60L IS VALUE OF SPIN D 

R 

E 

V 

r 

R 

T 

A 

N 

T 

C 

0 

u 

N 

T 

1500.C 

1400. 0 

1300. 0 

1203. 0 

1103. 0 

133C.C 

900. 0 

6CG.0 

700. 0 

6 0C.0 

500. 0 

40C C 

• 

• 

i 

i 

i 

i 

• 

t 

i 

• 

» 

i 

• 

; 

i 

• 

• 

t 

• 

i 

i 

• 

i 

i 

* 

i 

i 

• 

300. 0 

20C.C • 

100. 0 • 

I 

I 

0 . 0 • 

10 1C3 

DOSE IN MICROGRAMS 

1333 

.--- * y 

1 .'COO 
r 

T ' 

S:SOLVENT P:P0SITIVE N:ANTHRA*INE W/O S9 »:TEST COMPOUND 

C0N = 1°148 2 CHEM:MC STRAIN'slCO D A TE : 3 3 1 6 33 ACTIV:HEFA01 »CT* = 05 

PLOT OF AVCCOUNT«DOSE SYMPOL IS VALUE OF SPIND 

R 

E 

V 

r 

R 

T 

A 

N 

T 

C 

0 

u 

N 

T 

1800. 0 • 

1600. 0 • 

1400. C • 

1200. 3 • 

1 0 0 C 0 

60C 0 • 

60G.C 

400, 

200. 0 

C C • 

10 13C 

COSE I N MICROGRAM : 

IC! 

..... . 

1 CCC C 

65 
MUTAGENICIT Y OF FM - TA9 7 

P.74 

MUTAGENI C 

EXP DATE: 040783 

TEST TYPE: 05 

DILUTION: : 

MIXTURE: 

T Y 

CHEMICAL: FM 

STRAIN: TA97 

P.LATEO: 

TECHNICIAN: LGM 

CONTRAC T NO.: 

ACTIVATION : 

BATCH: DC63 

COLONIES: 

191482 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE 

COUNT 

A: 

6G 

PLATE B! 

COUNT BG 

PLATE C: 

COUNT BG 

AVERAGE 

COUNT 

STO DEV 

COUNT 

O / C MR MEC 

Sl 

F l 

P2 

99 

02 

02 

0.0 0 

50.0 0 

100.0 0 

1.C0 

5.0 0 

10.0 0 

50.0 0 

100.0 0 

153 

396 

577 

45 

78 

44 

106 

61 

U l 

503 

566 

42 

91 

73 

153 

94 

B9 

331 

595 

94 

95 

98 

137 

79 

117. 7 

410. 0 

579. 3 

60. 3 

68. 0 

71. 7 

132. 0 

78. 0 

32. 5 

86. 9 

14. 6 

29. 2 

8 . 9 

27.C 

23. 9 

16. 5 

A 

A 

A 

1.0 

3 . 5 

4 . 9 

0 . 5 

0 . 7 

0 . 6 

1 . 1 

0 . 7 

M U T A G 

E XP DATE : 

TES T TYPE 

DILUTION : 

E N I C 

040783 

I 05 

l: 20 

MIXTURE: 500 

I T Y 

CHEMICAL: FM 

STRAIN: TA97 

PLATED: ICO 

TECHNICIAN: LGM 

CONTRACT 'NO.: 191482 

ACTIVATION: CSDC02 

BATCH : 0063 

COLONIES: 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE 

COUNT 

A: 

BG 

PLATE 

COUNT 

B : 

BG 

PLATE C: 

COUNT BG 

AVERAGE 

COUNT 

STD DEV 

COUNT 

O/C MR MEC 

Sl 

PI 

P2 

99 

0 4 

C4 

C O O 

1.OJ0 

2.CC 

1.00 

5.00 

10.00 

50.CO 

103.00 

151 

654 

911 

70 

106 

63 

165 

lbl 

174 

886 

1217 

69 

113 

137 

143 

235 

181 

716 

11 "2 

88 

98 

136 

19* 

123 

16e. 7 

752. 0 

1106. 7 

75. 7 

105. 7 

118. 7 

174. 3 

169. 7 

15.7 

120.1 

169.9 

10.7 

7.5 

30.= 

27.6 

58.3 

1.0 

4.5 

6.6 

0-»4 

0.6 

0.7 

1.0 

1.3 

MUTAGENICIT Y 

EX» DATE: 040783 

TEST TYPE: 35 

DILUTION: i: 20 

MIXTURE: 530 

CHEMICAL : FM 

STRAIN : TA9 7 

PLATEC : 13 0 

TECHNICIAN : LG M 

CONTRAC T NO. : 19146 2 

ACTUATION : HEPAC I 

BATCH : DC63 

COLONIES: 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE 

COUNT 

A : 

BG 

PLATE e: 

COUNT FG 

PLATE C: 

COUNT 6G 

AVERAGE 

COUNT 

STD DEV 

COUNT 

0 /C MR MEC 

Sl

 Q 9 

f'l G4 

P2 04 

0.00 

0.50 

1.30 

1.00 

5.00 

10.00 

50.00 

100.30 

11° 

2S1 

793 

111 

116 

92 

136 

16S 

122 

435 

917 

122 

139 

116 

168 

196 

176 

39* 

763 

153 

171 

177 

2 01 

157 

139. 0 

373. 7 

630. 0 

127. 7 

142. 0 

128. 3 

169. 0 

173. 7 

Zc. I 

74. 3 

75. 6 

20. 1 

27. 6 

43. 6 

31. 5 

20. 1 

I.C 

2

'

7

| 6 . 0 

0.9 

1 . 0 

0.9; 

1.2 

66 
f* B 

*-i."j,tt«. *!•'*«*; I 

S:SOLVENT P:P0SITIVE N:ANTHRAMINE y/ 0 S9 *=TEST COMPOUND 

£QN:19148 2 CHEM:FM STRAIN:97 DATE:040783 ACTIV: ACT*:C5 

PLOT OF AVGCOUNT*DOSE SYMBOL IS VALUE OF SPIND 

R 

E 

V 

r 

R 

T 

A 

N 

T 

C 

0 

u 

N 

T 

900. 0 • 

• 

; 

I 

! 

800. 0 • 

700. 0 • 

600. 0 • 

500. 0 • 

HOC O • 

300. 0 • 

200. 0 • 

100. 0 • 

C O • 

1 0 10 0 

•3SE IN MICROGRAMS 

1 Cl- 0 

..... . 

iicco o 

67 
P.76 

i : 

S:$OLVENT P=P0SITIVE N:ANTHRAMINE W/O S9 «:TEST COMPOUND 

C0N:1914B2 CHEM:FM STRAIN:97 DATE:P40783 ACTIV:CSD002 ACT*: 05 

PLOT OF AVGCOUNT«DOSE SYMBOL IS VALUE OF SPIND 

i 

i 

1100.0 • 

I 

! 

R 

r 

V 

r 

R 

T 

A 

N 

T 

C 

0 

U 

N 

T 

1000.0 • 

• 

i 

i 

900.0 • 

i 

• 

i 

800.0 • 

; 

i 

i 

700.0 • 

; 

; 

i 

6 00.9 • 

; 

i 

500.0 • 

i 

; 

i 

400.0 • 

i 

i 

i 

300.0 • 

i 

; 

i 

200.0 • 

! S 

i 

i 

100.0 • 

I 

I 

I 

0.0 • 

10 ICG 

DOSE IN MICROGRAMS 

1333 

4 - -. 4 . 

i cooc 
P.77 

S:SOLVENT P:P0SITIV E N:ANTHRAMINE W/O S9 *=TES T COMPOUND 

C0N:19148 2 CHEM:FM STRAIN:9 7 DATE:04076 3 ACTlV:HEFA0 1 ACTX:0 5 

PLOT OF AVGCOUNT'DOSE SYMBOL I S VALUE OF SPIN D 

R 

r 

V 

E 

R 

T 

A 

N 

T 

C 

0 

u 

N 

T 

900. 0 • 

800. 0 • 

7U0.C • 

600. 0 • 

500. 0 • 

400. 0 * 

300. 0 • 

200. 0 • 

100. 0 • 

CO • 

10 1 0 0 ico o 1 oCO O 

DOSE I N MICROGRAMS 

69 
MUTAGENICIT Y OF FM - TA9 8 

P.78 

MUTAGENICIT Y 

EXP DATE: 040783 CHEMICAL : FM 

TEST TYPE: 05 STRAIN: TA98 

DILUTION: : PLATED= 

MIXT"D.r= TE-CHNICIAN: LGM 

CONTRAC T NO. : 19146 2 

ACTIVATION : 

BATCH : D063 

COLONIES: 

SO.VENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE A: 

COUNT BG 

PLATE B: 

COUNT BG 

PLATE C: 

COUNT BG 

AVERAGE 

COUNT 

STO DEV 

COUNT 

G/C MR MEC 

Sl 99 

Fl G3 

P2 03 

0.00 

1.00 

2.00 

l.GO 

5.0 0 

10.00 

50.00 

100.00 

19 

82 

222 

25 

16 

31 

24 

56 

19 

139 

341 

24 

19 

27 

27 

26 

17 

14? 

267 

14 

23 

35 

22 

32 

18.3 

121.0 

276.7 

21.0 

19.3 

31.0 

24.3 

38.0 

1.2 

33.8 

60.1 

6.1 

3.5 

4.0 

2.5 

15.9 

1.0 

6.6 

15.1 

1.1 

1.1 

1.7 

1.3 

2.1 

M U T A G 

EXP DATE : 

TEST TYPE! 

DILUTION: 

E N I C 

040783 

05 

i: 20 

MIXTURE: 500 

I T Y 

CHEMICAL : FM 

STRAIN : TA98 

PLATED: 100 

TECHNICIAN: LGM 

CONTRAC T NO. 

ACTIVATION : 

BATCH : DC63 

COLONIES: 

: 1914 82 

CSD002 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE 

COUNT 

A: 

BG 

PLATE B : 

COUNT BG 

PLATE C: 

CCUNT EG 

AVERAGE 

COUNT 

STD DEV 

COUNT 

G/C MR MEC 

Sl 99 

PI 34 

P2 04 

0.00 

1.00 

2.00 

1.00 

5.00 

ICO'J 

50.00 

100.00 

22 

793 

1567 

33 

23 

25 

35 

30 

32 

666 

1696 

31 

33 

30 

44 

37 

23 

679 

1573 

27 

29 

36 

30 

47 

25.7 

779.3 

1612.0 

30.3 

28.3 

31.0 

36.3 

38.0 

5.5 

94.2 

72.6 

3.1 

5.0 

6.6 

7.1 

8.5 

1.0 

30.3 

62.7 

1.2 

1.1 

1.2 

1.4 

1.5 

MUTAGENICIT Y 

EXP DATE: 040783 

TEST TYPE: 35 

DILUTION: l: 20 

MIXTURE: 500 

CHEMICAL : F* 

STRAIN : TA96 

PLATEC: ICO 

TECHNICIAN: LGM 

CONTRAC T NO. : 19148 2 

ACTIVATION : HEPAC I 

BATCH: DC63 

COLONIES: 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE A: 

COUNT EG 

PLATE B: 

COUNT BG 

PLATE Ct 

COUNT FG 

AVERAGE 

COUNT 

STD DEV 

COUNT 

O/C

 VR MEC 

Sl 

PI 

P2 

99 

04 

C4 

C O O 

0.59 

1.00 

1.00 

5.00 

10.CO 

50.00 

100.00 

41 

i9e 

747 

29 

22 

41 

35 

3 5 

35 

262 

eoe 

42 

33 

36 

26 

49 

26 

236 

672 

37 

42 

4C 

36 

36 

34. 3 

232. 7 

742. 3 

36. 0 

32. 3 

39. 0 

32. 3 

40. 0 

7 . 5 

32. 3 

68. 1 

6 . 6 

I C C 

2.6 ' 

5 . 5 

7 . 8 

1 . 0 

6 . 8 

21. S 

1 . 1 

1 . 3 

1 . 1 

1 . 3 

1 . 2 

70 
P.79 

' I ' i 

S=SOLVENT P=P0SITIVE NsANTHRAMINE W/O S9 *:TEST COMPOUND 

C0N:191482 CHEM:FM STRAIN:98 DATE=040783 ACTIV: ACTX:05 

PLOT OF AVGCOUNT*DOSE SYMBOL IS VALUE OF SPIND 

R 

E 

V 

E 

R 

T 

A 

N 

T 

C 

0 

u 

N 

T 

275.0 • 

250.0 • 

225.0 

200. C 

175.0 

150.0 

125.0 

100.0 

75.C 

50.C 

25.0 

0.0 • 

10 ICO 

DCSE IN MICROGRAMS 

1C0O 

...... 

10000 

71 
S=SOLVENT P:P0SITIVE N:ANTHRAMINE W/O S9 *:TEST COMPOUND 

C0N=191492 CHEM:FM STRAIN:98 DATE=040783 ACTIV:CSD002 ACTX:05 

PLOT OF AVGCOUNT«DOSE SYMBOL IS VALUE OF SPIND 

P.80 

R 

E 

V 

E 

R 

T 

A 

N 

T 

C 

0 

u 

N 

T 

1800.0 • 

t 

; 

! 

; 

1600.0 • 

1400.0 • 

; 

! 

i 

i 

1200.0 • 

1900.0 • 

8.00.0 

600.0 • 

400.0 • 

23C.0 • 

0.0 • 

10 100 

DOSE IN MICROGRAMS 

1000 1 3000 

72 
P.81 

S:S0LVENT P:POSlTIVE N : ANTHP. A* INE W/O S9 *:TEST COMPOUND 

C0N:191482 CHEM:FM STRAIN:98 DATE:040783 ACTlV:HEPA01 ACTX:05 

PLOT OF AVGCOUNT»DO',E SYMBOL IS VALUE OF SPIND 

P 

r 

V 

E 

R 

T 

A 

N 

T 

C 

0 

U 

N 

T 

! 

900. 0 • 

800. 0 • 

700. 0 • 

! 

600. 0 • 

5C0. O 

400. 9 • 

300. 0 

200. 0 

1 00. 0 

0 . 0 • 

10 100 

DOSE IN MICROGRAMS 

1000 10CC0 

73 
MUTAGENICIT Y OF FM - TAlO O 

M U T A G 

ZXP DATE : 

TEST TYPE 

DILUTION : 

MIXTURE: 

E N 1 C 

040783 

: 05 

T Y 

CHEMICAL : 

STRAIN : TA 

PLATED: 

TECHNICIAN 

FM 

100 

CONTRAC T NO. : 19148 2 

ACTIVATION : 

BATCH : D063 

COLONIES: 

LGM 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE 

COUNT 

A: 

BG 

PLATE B: 

COUNT BG 

PLATE 

COUNT 

c: 

BG 

AVERAGE 

COUNT 

STD DEV 

COUNT 

O/C MR MEC 

Sl 

PI 

P2 

99 

01 

01 

0.00 

1.00 

2.00 

1.00 

5.00 

10.00 

50.00 

IOC.00 

122 

278 

587 

133 

137 

139 

81 

174 

92 

284 

493 

113 

71 

106 

80 

165 

114 

239 

406 

8* 

11* 

84 

74 

131 

109. 3 

267. 0 

495. 3 

110. 3 

107. 7 

109. 7 

78. 3 

156. 7 

15.5 

24.4 

90.5 

24.1 

33.6 

27.7 

3.8 

22.7 

1.0 

2.4 

4.5 

1.0 

1.0 

). .0 

0.7 

1.4 

M U T A G 

EXP DATE : 

TEST TYPE: 

DILUTION: 

E N I C 

040783 

05 

i: 20 

MIXTURE: 500 

I T Y 

CHEMICAL : FM 

' STRAIN : TAlOO 

PLATEC: 100 

TECHNICIAN: LGM 

CONTRACT NO.: 191482 

ACTIVATION: CSD002 

BATCH: D963 

COLONIES: 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE A: 

COUNT BG 

PLATE BI 

COUNT BG 

PLATE 

COUNT 

c: 

BG 

AVERAGE 

COUNT 

STD DEV 

COUNT 

Q/C MR MEC 

Sl 99 

PI 04 

P2 C4 

C O O 

1 . CO 

2.00 

1.00 

5.00 

10.00 

50.00 

100.00 

143 

690 

1506 

83 

77 

124 

128 

233 

167 

970 

1999 

89 

61 

106 

152 

174 

143 

922 

1720 

63 

7E 

93 

129 

133 

151.0 

860.7 

1741.7 

85.0 

72.0 

107.7 

136.3 

160.0 

13.9 

149.7 

247.2 

3.5 

9.5 

15.6 

13.6 

50.3 

1.0 

5.7 

11.5 

0.6 

0.5 

0.7 

0.9 

1.2 

M U T A G 

EXP DATE : 

TEST TYPE : 

DILUTION: 

E N I C 

040783 

05 

i: 2C 

MIXTURE: 5C3 

I T Y 

CHEMICAL : F M 

STRAIN : TAIO O 

'PLATED: 130 

TECHNICIAN: LGM 

CONTRACT NO.: 1914fc2 

ACTIVATION : HEPAC I 

BATCH: D363 

COLONIES: 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE 

COUNT 

A : 

BG 

PLATE 

CCUNT 

B : 

FG 

PLATE C. 

COUNT BG 

AVERAGE 

COUNT 

STD DEV 

CCUNT 

O/C M.R MEC 

Sl 

PI 

P2 

99 

04 

04 

C O O 

0.50 

1.03 

1.00 

5.00 

10.00 

50.00 

100.00 

13C 

302 

236 

186 

196 

10 6 

136 

147 

120 

417 

231 

193 

190 

107 

68 

182 

111 

456 

367 

160 

151 

11 1 

75 

126 

120. 3 

392. 3 

278. 0 

179. 7 

179. 0 

106. 7 

93. 7 

151. 7 

9 . 5 

6C 9 

77. 1 

17. 4 

24. 4 

2 . 1 

36. 6 

26. 3 

I . C 

3 . 3 

2«o 

1.5 

1.5 

0 . c 

3 . 6 

1 . 3 
S:S0LVENT P:P0SITIVE N=ANTHRAMINE W/O S9 «:TEST COMPOUND 

COW:191482 CHEM:FM STRAIN:100 DATE:040783 ACTIV: ACTX:D5 

PLOT OF AVGCOUNT*DOSE STMBOL IS VALUE OF SPIND 

R 

Z 

V 

E 

R 

T 

A 

N 

T 

C 

0 

u 

N 

T 

; 

t 

550. 0 • 

! 

! 

; 

500. 0 • 

450. D • 

400. 0 • 

350. 0 • 

300. 0 • 

250. 0 • 

200. 0 • 

150. 0 • 

100. 0 • 

50. 0 • 

0 . 0 • 

1 0 IC O 

DOS E IN MICROGRAM S 

100 0 1 C 0 0 0 

75 
P.84 

S:S0LVEN T PsPOSITIV E N:ANTHRAMINE U/C S9 «:TES T COMPOUND 

C0N:19148 2 CHEM:FM STRAIN:10 0 DATE:04078 3 ACTIV:CSD00 2 ACTX:0 5 

PLOT OF AVGCOUNT«DOSE SYMBOL I S VALUE OF SPIN D 

R 

r 

V 

E 

R 

T 

A 

N 

T 

C 

0 

u 

N 

T 

1800. 0 * 

• 

! 

! 

i 

1600. 0 • 

! 

• 

; 

! 

1400. 0 • 

! 

1200. 0 • 

10CC Q • 

800. 0 • 

600. 0 • 

400. 0 • 

200. 0 • 

0 . 0 • 

10 1 0 J 

DCSE I N MICROGRAMS 

10C0 

• — • . 

;o oo 
P.85 

S:SOLVENT P=POSITIVE N:ANTHRAMINE W/O S9 *:TEST COMPOUND 

C0N=191482 CHEM:FM STRAIN:100 DATE=040763 ACTlV=HEPA01 ACTX:05 

PLOT OF AVGCOUNT.DOSE SYMBOL IS VALUE OF SPIND 

R 

E 

V 

r 

R 

T 

A 

N 

T 

C 

0 

u 

450.0 • 

! 

400.0 • 

350.0 • 

i 

! 

! 

; 

300.0 • 

250.0 • 

200.0 • 

150.0 • 

100.0 • 

50.0 • 

0.0 • 

10 ICC 

DOSE IN MICROGRAMS 

ICO 

...... 

1 00 01 
-r---

MUTAGENICIT Y OF FRC - TA9 7 

P.86 

MUTAGENICIT Y 

EXF DATE: 040783 CHEMICAL: TRC 

TEST TYPE: 05 STRAIN: TA97 

DILUTION: : PLATED: 

MIXTURE: TECHNICIAN: LGM 

CONTRAC T NO. : 19148 2 

ACTIVATION : 

BATCH: D063 

COLONIES: 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE A: 

COUNT BG 

PLATE B: 

COUNT PG 

PLATE 

COUNT 

c: 

BG 

AVERAGE 

COUNT 

STD DEV 

CCUNT 

O/C MR MEC 

Sl 99 

PI 02 

P2 02 

0.0 0 

50.0 0 

100.0 0 

3.0 0 

10.0 0 

33.0 0 

100.0 0 

333.0 0 

153 

396 

577 

64 

50 

131 

114 

36 

U l 

503 

566 

120 

65 

149 

78 

78 

89 

331 

595 

91 

129 

127 

116 

75 

117. 7 

410. 0 

579. 3 

98. 3 

81. 3 

135. 7 

102. 7 

63. 0 

32. 5 

86. 9 

14. 6 

19. 1 

42. 0 

11. 7 

21. 4 

23. 4 

A 

A 

A 

1.0 

3 . 5 

4 . 9 

0 . 8 

0 . 7 

1.2 

0 . 9 

0 . 5 

M U T A G 

EXP DATE : 

TEST TYPE 

DILUTION: 

E N I C 

04C783 

: 05 

i: 20 

MIXTURE: 500 

T Y 

CHEMICAL: TRC 

STRAIN: TA97 

PLATED: 100 

TECHNICIAN: LGM 

CONTRACT NO.: 191482 

ACTIVATION: CSD002 

BATCH: 0363 

COLONIES: 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE 

COUNT 

A : 

BG 

PLATE P: 

COUNT CG 

PLAT E 

COUN T 

c: 

BG 

AVERAGE 

CCUNT 

STD DEV 

COUNT 

O/C MR MEC 

Sl 

Fl 

P2 

99 

04 

04 

0.00 

1.00 

2.00 

3.00 

10.00 

33.CO 

100.00 

333.00 

151 

654 

911 

178 

98 

U l 

64 

115 

174 

886 

1217 

135 

91 

142 

128 

128 

161 

716 

1192 

92 

1*1 

133 

96 

9 2 

163. 7 

752. 0 

1136. 7 

135. 0 

110. 0 

127. 7 

96. 0 

111. 7 

15.7 

120.1 

169.9 

43.0 

27.1 

15.6 

32.0 

16.2 

1.0 

4.5 

6.6 

0.6 

0.7 

0.8 

0.6 

0.7 

M U T A G 

EXP DATE: 

TEST TYPE 

DILUTION: 

E N I C 

C40783 

: 05 

i: 20 

FIXTURE: 500 

I T Y 

CHEMICAL : TRC 

STRAIN : TA97 

PLATEC: 130 

TECHNICIAN: LGM 

CONTRACT NO.: 19148? 

ACTIVATION : HEPAC1 

BATCH : D063 

COLONIES: 

SOLVENT/ 

POSITIV E 

DOSE 

LEVEL 

PLATE 

COUNT 

A : 

BG 

PLATE 

COUNT 

B : 

BG 

PLATE C: 

COUNT BG 

AVERAGE 

COUNT 

STD DLV 

COUNT 

C/C *'R MEC 

Sl 

PI 

P2 

99 

04 

C4 

0.00 

0.50 

l.CC 

3.00 

10.CO 

3 3 • w o 

103.00 

333.CO 

119 

291 

793 

143 

81 

151 

69 

123 

122 

435 

917 

112 

143 

157 

96 

146 

176 

395 

763 

106 

152 

131 

132 

13C 

13°.C 

373. 7 

830. 0 

120. 3 

125. 3 

146. 3 

106. 3 

133. 0 

32. 1 

74. 3 

75. 6 

19. 9 

36.'7 

13. 6 

22. 7 

11. 6 

I . C 

2 . 7 

6 . 0 

0.9' 

0 . 9 

1.1 

C H 

1 . 0 

76 
P.87 

S=SOLVENT P:P0SITIVE N=ANTHRAM1NE W/O S9 *:TEST COMPOUND 

C0N=191482 CHEH=TRC STPAIN:97 DATE:040783 ACTIV: ACTX:05 

PLOT OF AVGCOUNT«DOSE SYMBOL IS VALUE OF SPIND 

R 

E 

V 

E 

R 

T 

A 

N 

T 

C 

0 

u 

N 

T 

900. 0 • 

i 

i 

! 

! 

800.0 • 

700.0 • 

600. 0 • 

500. 9 • 

400. 0 • 

300. 0 • 

200. 0 • 

100. 0 • 

0 . 0 • 

10 100 

DOSE IN MICROGRAMS 

130C 1C0C0 

79 
P.88 

9 

r 

V 

E 

R 

T 

A 

N 

T 

C 

0 

u 

N 

T 

S=SOLVENT PsPOSITIVE NsANTHRAMINE W/O S9 «:TEST COMPOUND 

C0N:19148 2 CHEM.:TRC STRAIN:97 DATE:040783 ACTIV:cSD002 ACTX=05 

PLOT OF AVGCOUNT*DOSE SYMBOL IS VALUE OF SPIND 

i 

i 

1100. 0 • P 

1000. 0 • 

900. 0 • 

800. 0 • 

700. 0 • 

600. 0 • 

500. 0 • 

400. 0 • 

300. 0 • 

200. 0 • 

100. 0 • 

C O • 

10 100 

DOSE IN MICROGRAMS 

100 0 ! C C 0 0 

80 
P.89 

S:SOLVENT P:P0SITIVE NsANTHRAMINE W/O S9 «:TEST COMPOUND 

C0N:191482. . CHEM:TRC STRAIN:97 DATE:040783 ACTIV:HEPA01 ACTX :05 

PLOT OF AVGCOUNT«DOSE SYMBOL IS VALUE OF SPIND 

R 

r 

V 

E 

R 

T 

A 

N 

T 

f; 

0 

U 

N 

T 

900. 0 

800. 0 

700. 0 

600. 0 

500. 0 

400. 0 

300. 0 

200 . 0 

100. 0 

C O 

i 

I 

; 

• 

; 

i 

t 

; 

i 

! 

• 

• 

i 

• 

i 

• 

• 

i 

i 

t 

i 

• 

\ 

i 

• 

• 

• 

i 

i 

; 

i 

• 

; • 

• S 

• 

• 

i 

i 

; 

• 

• 

10 100 

COSE IN MICROGRAMS 

1C0 0. 

...... 

i :oo 

81 
MUTAGENICITY OF TRC - TA98 

MUTAGENI C 

EXP DATE: 040783 

TEST TYPE: 05 

DILUTION: : 

MIXTURE= 

T Y 

CHEMICAL : TRC 

STRAIN: TA98 

PLATED: 

TECHNICIANS LGM 

CONTRACT NO.: 191482 

ACTIVATION: 

BATCH: D063 

COLONIES: 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE A: 

COUNT BG 

PLATE B: 

COUNT BG 

PLATE 

COUNT 

c: 

BG 

AVERAGE 

COUNT 

STD DEV 

COUNT 

C/C HR MEC 

Sl 59 

PI 03 

P2 03 

0.00 

1.00 

2.00 

3.00 

10.GO 

33.00 

100.00 

333.00 

19 

82 

222 

15 

27 

16 

17 

45 

19 

139 

341 

20 

26 

17 

12 

20 

17 

142 

267 

17 

18 

17 

17 

22 

18.3 

121.0 

276.7 

17.3 

23.7 

16.7 

15.3 

29.0 

1.2 

33.8 

60.1 

2.5 

4.9 

0.6 

2.9 

13.9 

A 

A 

A 

1.0 

6.6 

15.1 

0.9 

1.3 

0.9 

4.8 

1.6 

M U T A G 

E XP DATE : 

TES T TYPE: 

DILUTION : 

E N I C 

040783 

05 

i: 20 

MIXTURE: 5C0 

I T Y 

CHEMICAL : TRC 

STRAIN.* TA98 

PLATED: ICO 

TECHNICIAN: LGM 

CONTRACT NO.: 191482 

ACTIVATION: CSD002 

BATCH : D063 

COLONIES: 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE 

COUNT 

A: 

BG 

PLATE B: 

COUNT BG 

PLATE 

COUNT 

c: 

EG 

AVERAGE 

COUNT 

STD DEV 

COUNT 

O/C MR MEC 

Sl 99 

PI 04 

P2 04 

0.00 

1.00 

2.00 

3.00 

10.00 

33.00 

100.00 

333.00 

22 

793 

1567 

24 

24 

21 

36 

41 

32 

866 

1696 

36 

29 

29 

21 

31 

23 

679 

1573 

31 

21 

31 

34 

34 

25.7 

779.3 

1612.0 

30.3 

24.7 

27.0 

30.3 

35.3 

5.5 

94.2 

72.8 

6.0 

4.3 

ii. 3 

rt.l 

5.1 

1.0 

30.3 

62.7 

1.2 

1.0 

1.1 

1.2 

1.4 

MUTAGENICIT Y 

EXP DATE: 040783 

TEST TYPE: 05 

DILUTION: l: 20 

MIXTURE: SCO 

CHEMICAL : TRC 

STRAIN: TA98 

PLATED: 100 

TECHNICIAN: LGM 

CONTRACT NO.: 1914e2 

ACTIVATION : HEPAC I 

BATCH: 0063 

COLONIES: 

SOLVENT/ 

POSITIVE 

OOSE 

LEVEL 

PLATE A: 

COUNT BG 

PLATE B : 

COUNT CG 

PLATE c: 

COUNT BG 

AVERAGE 

COUNT 

STD DEV 

COUNT 

G/C MR 

S l 9 9 

F l 34 

P2 04 

C C O 

Q.5 0 

1.C0 

3.0 0 

10.0 0 

33.0 0 

100.0 0 

333.0 0 

41 

196 

747 

25 

22 

40 

27 

34 

35 

262 

6 0 6 

27 

36 

28 

23 

38 

26 

236 

672 

30 

26 

40 

4 1 

40 

34. 3 

232. 7 

742. 3 

27. 3 

28. 0 

36. 0 

3 0. 3 

37. 3 

7 . 5 

32. 3 

66. 1 

2 . 5 

7 . 2 

6 . 9 

9 . 5 

3 . 1 

6.8. 

21 . 8 

o.'e 

C R 

1.1 

0 . 9 

1.1 

82 
P.91 

S:S0LVENT PsPOSITIVE NsANTHRAKINE W/O S9 *:TEST COMPOUND 

C0N:191482 CHEMsTRC STRAIN:98 DATE:040783 ACTIV: ACTX:05 

PLOT OF AVGCOUNT*DOSE SYMBOL IS VALUE OF SPIND 

R 

E 

V 

E 

R 

T 

A 

N 

T 

C 

0 

U 

N 

T 

275. 0 • 

250. 0 

225. 0 

I 

200. 0 • 

i 

i 

175. 0 • 

• 

i 

i 

150. 0 • 

i 

i 

i 

125. 0 • 

i 

i 

i 

100. c • 

I 

I 

I 

75. 0 • 

i 

i 

• 

50. 0 • 

; 

t 

i 

25. C • 

! S 

i 

i 

C O • 

10 IOC 

DOSE I N MICROGRAMS 

100 0 

. . - - • -

ICOOO 

83 
P.92 

S:SOLVENT PsPOSITIVE NsANTHRAMINE W/O S9 *=TEST COMPOUND 

C0N:191482 CHEM:TRC STRAIN:9e OATE:040783 ACTIV:cSD002 ACTX:05 

PLOT OF AVGCOUNT.DOSE SYMBOL IS VALUE OF SPIND 

R 

E 

V 

E 

R 

T 

A 

N 

T 

C 

0 

U 

N 

T 

1800.0 • 

• 

! 

i 

i 

1600.0 • 

! 

1400.C • 

1200.0 

1000.0 • 

800.0 • 

600.0 • 

400.0 • 

200.0 • 

0.0 • 

10 

... -. 

100 1000 13 0 0 0 

DOSE IN MICROGRAM S 

84 
P.93 

SsSOLVENT PsPOSITIVE NsANTHRAMINE W/O S9 *:TEST COMPOUND 

C0N=191482 CHEM:TRC STRAIN=98 DATE=040783 ACTIVsHEPAOl ACTX:05 

PLOT OF AVGCOUNT.DOSE SYMBOL IS VALUE OF SPIND 

i 

900. 0 • 

; 

i 

; 

i 

800. 0 • 

; 

i 

! P 

; 

700. 0 • 

• 

! 

i ' 

i 

R 600. 0 • ' 

E ! 

V ! 

E ! 

a ! 

T 500. 0 • 

A ! 

N ! 

T ! 

; 

C 400. 0 • 

0 ! 

U » 

N ! ! 

T ! i 

300. 0 • 

i 

i 

• P 

! i 

200. 0 • 

; 

; 

100. 0 • 

I 

I 

! S * * * 

; * * 

0 . 0 • 

— • • •-.. - • -—•.-.- - ,-.-• . 

0 , 1 10 ICC 1000 13000 

DOSE IN MICROGRAMS 

85 
P.94 

MUTAGENICITY OF TRC TAlOO 

MUTAGENI C 

EXP DATE: 040783 

TEST TYPE: 05 

DILUTION : : 

MIXTURES 

T Y 

CHEMICAL: TRC 

STRAIN: TAlOO 

PLATED= 

TECHNICIAN: LGM 

CONTRACT NO.: 

ACTIVATION: 

BATCH: 0063 

COLONIES: 

191482 

SOLVENT/ DOSE PLATE A: PLATE B: PLATE C: AVERAGE STD DEV 

POSITIVE LEVEL COUNT BG COUNT BG COUNT EG COUNT COUNT 

O/C MR MEC 

Sl 99 

PI 01 

F2 01 

COO 

1.00 

2.00 

3.00 

10.00 

33.00 

100.00 

333.00 

122 

278 

587 

130 

144 

82 

124 

121 

92 

284 

493 

75 

107 

84 

114 

92 

114 

239 

406 

83 

106 

76 

115 

97 

109.3 

267.0 

495.3 

96.0 

119.0 

80.7 

117.7 

103.3 

15.5 

24.4 

90.5 

29.7 

21.7 

4.2 

5.5 

15.5 

A 

A 

A 

1.0 

2.4 

4.5 

0.9 

1.1 

0.7 

1.1 

0.9 

MUTAGENI C 

EXP DATE: 040763 

TEST TYPE: 05 

DILUTION: It 20 

MIXTURE: 500 

T Y 

CHEMICAL: TRC 

STRAIN: TAlOO 

PLATED: 100 

TECHNICIAN: LGM 

CONTRACT NO.:.191482 

ACTIVATION,: CSD0C2 

BATCH-: D06 3 

COLONIES: 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE A: 

COUNT BG 

PLATE B: 

COUNT BG 

PLATE 

COUNT 

c: 

BG 

AVERAGE 

COUNT 

STD DEV 

COUNT 

O/C MR MEC 

S l 

Fl 

P2 

99 

04 

04 

CCO 

1.0 0 

2.0 0 

3.0 0 

1CC 0 

33.0 0 

100.0 0 

333.0 0 

14 3 

690 

1506 

1 2 8 

1 0 8 

68 

150 

88 

167 

970 

1999 

1 2 5 

1 2 3 

63 

84 

68 

1 4 3 

922 

172 0 

86 

72 

42 

1 0 7 

151. C 

860. 7 

1741. 7 

113. 0 

131. 0 

57. 7 

113. 7 

78. 0 

13. 9 

149. 7 

247. 2 

23. 4 

26. 2 

13. 8 

33. 5 

14. 1 

A 

A 

A 

1 . 0 

5 . 7 

11. 5 

0 . 7 

0 . 7 

0.4 

C 8 

0 . 5 

M U T A G 

EXP DATE: 

TEST TYP: 

DILUTION: 

E N I C 

040783 

05 

i: 20 

MIXTURE: 500 

I T Y 

CHEMICAL: TRC 

STRAIN: TAlOO 

PLATED: 100 

TECHNICIAN: LGM 

CONTRACT NO.: 191482 

ACTIVATION: HEPACI 

BATCH: DC63 

COLONIES: 

SOLVENT/ DOSE PLATE A: PLATE B: PLATE C: AVERAGE STD DEV 

POSITIVE LEVEL COUNT BG COUNT BG CCUNT PG COUNT CCUNT 

O/C

 WF. MEC 

Sl 

PI 

P2 

99 

04 

C4 

0.00 

0.50 

1.00 

3.00 

10.00 

33.00 

100.00 

333.00 

130 

3C2 

236 

163 

177 

150 

145 

167 

120 

417 

231 

159 

191 

188 

220 

174 

U l 

458 

367 

151 

167 

177 

185 

147 

120.3 

392.3 

278.0 

16 4.3 

176.3 

171.7 

ie3.3 

162.7 

9.5 

RC.9 

77.1 

16.7 

12.1 

19.6 

37.5 

14.0 

1.0 

3.3 

2.3 

1.4 

1.5 

1.4 

1.5 

1.4 

86 
P.95 

SsSOLVENT P:P0SITIVE NsANTHRAMINE W/O S9 »sTEST COMPOUND 

C0N:191482 CHEM:TRC STRAINMOO DATE = 040783 ACTIV: ACTx':05 

PLOT OF AVGCOUNT*DOSE SYMBOL IS VALUE OF SPIND 

R 

E 

V 

E 

R 

T 

A 

N 

T 

C 

t) 

U 

N 

T 

i 

i 

550.0 • 

i 

t 

! 

500.0 • 

450.0 • 

400.0 • 

350.0 • 

300.0 • 

250.0 • 

200.0 • 

150.0 • 

100.0 • 

50.0 • 

0.0 • 

io l ;o 

DOSE IN MI 'ROGRAMS 

uo; 10 0 0 0 
SssOLVENT PsPOSITIVE NsANTHRAMINE W/O S9 .sTEST COMPOUND 

C0Nsl91482 CHEMsTRC STRAINslOO DATEs040783 ACTIV:CSD00 2 ACTX:05 

PLOT OF AVGCOUNT»DCSE SYMBOL IS VALUE OF SPIND 

P.96 

R 

E 

V 

E 

R 

T 

A 

N 

T 

C 

0 

U 

N 

T 

1800.0 • 

» 

• 

! 

t 

1600.0 • 

! 

! 

i 

i 

1400.0 • 

12C0.0 • 

1000.0 

800.0 • 

600.0 • 

400.0 • 

200.0 • 

0.0 • 

10 

... _. 

100 1P00 10000 

DOSE IN'MICROGRAMS 

88 
SsSOLVENT PsPOSITIVE NsANTHRAMINE W/O S9 *=TEST COMPOUND 

C0NS191482 CHEHsTRC STRAINslOO OATEs040783 ACTIVsHEPAOl ACTXr05 

PLOT OF AVGCOUNT.DOSE SYMBOL IS VALUE OF SPIND 

450.0 • 

R 

E 

V 

E 

R 

T 

A 

N 

T 

C 

0 

u 

N 

T 

400. 0 

350. 0 

300. 0 

250. 0 

2 00. 0 

/ 

150. 0 

! 

4 

1 

• 

! 

• 

• 

• 

100.0 • 

50.0 

0.0 • 

10 ioo 

DOSE IN MICROGRAMS 

1009 

ii 

11 

89 
.VST MUTAGENICITY OF EDC TA97 

MUTAGENI C 

EXP DATE.' 040883 

TEST TYPE: 05 

DILUTION : : 

MIXTURE: 

I T Y 

CHEMICAL : EDC 

STRAIN: TA97 

PLATED= 

TECHNICIAN: LGM 

CONTRAC T NO. : 19148 2 

ACTIVATION : 

BATCH : D073 

COLONIES: 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE 

COUNT 

A : 

BG 

PLATE B: 

COUNT BG 

PLATE C: 

COUNT BG 

AVERAGE 

COUNT 

STD DEV 

COUNT 

Q/C MR ME 

Sl 99 

PI 02 

P2 02 

0.00 

50.00 

100.00 

50.00 

100.00 

250.00 

500.00 

ICOO.OO 

188 

735 

1875 

192 

229 

187 

176 

202 

193 

832 

2285 

203 

196 

192 

202 

219 

194 

664 

2249 

230 

222 

165 

167 

186 

191. 7 

743. 7 

2136. 3 

208. 3 

215. 7 

181. 3 

181. 7 

202. 3 

3.2 

B4.3 

227.0 

19.6 

17.4 

14.4 

18.2 

16.5 

A 

A 

A 

1.0 

3.9 

U . l 

1.1 

1.1 

0.9 

0.9 

1.1 

M U T A G 

EXP DATE : 

TEST TYPE 

DILUTION: 

E N I C 

040683 

: 05 

i: 20 

MIXTURE: 590 

I T Y 

CHEMICAL : EDC 

STRAIN: TA97 

PLATED: 100 

TECHNICIAN: LGM 

CONTRAC T NO. : 15148 2 

ACTIVATION: CSDOOI 

BATCH : D073 

COLONIES: 

SOLVENT/ DOSE PLATE A: PLATE B: PLATE C: AVERAGE STD DEV 

POSITIVE LEVEL COUNT BG COUNT BG COUNT EG COUNT COUNT 

O/C MR ME 

Sl 

PI 

P2 

99 

04 

04 

0.00 

1.00 

2.00 

50.00 

100.CO 

250.00 

500.00 

ICOO.OO 

228 

678 

1713 

217 

19> 

165 

214 

233 

215 

703 

1608 

163 

253 

164 

262 

195 

225 

698 

1673 

214 

213 

182 

243 

0 

222. 7 

6^3. 0 

1798. 0 

198. 0 

220. 0 

177. 0 

239. 7 

142. 7 

6.8 

13.2 

80.5 

30.3 

30.1 

11.4 

24.2 

125.0 

1.0 

3.1 

8.1 

0.9 

I.G 

0.6 

1.1 

0.6 

MUTAGENICIT Y 

EXP DATE: 040883 

TEST TYPE: 05 

DILUTION: i: 20 

MIXTURE: 590 

SOLVENT/ 

POSITIVE 

DCSE 

LEVEL 

CHEMICAL : EDC 

STRAT f A97 

PLATED: 130 

TECHNICIAN: 

PLATE 

COUNT 

A: 

BG 

PLATE 

COUNT 

LGM 

B : 

BG 

CONTRAC T NO. : 19148 2 

ACTIVATION : HEPAO I 

BATCH : DC73 

COLONIES: 

PLATE 

COUNT 

c: 

BG 

AVERAGE 

COUNT 

STD DEV 

CCUNT 

Q/C MR ME 

Sl 

PI 

P2 

99 

04 

04 

0.00 

0.50 

1.C0 

50.00 

100.00 

250.00 

500.00 

300.00 

242 

376 

1135 

226 

212 

243 

289 

9 

199 

344 

1147 

191 

244 

214 

285 

173 

181 

359 

1059 

230 

233 

214 

222 

164 

207.3 

360.3 

1127.0 

215.7 

229.7 

223.7 

265.3 

122.0 

31.3 

17.0 

25.0 

21.5 

16.3 

16.7 

37.6 

98.0 

I.C 

1.7 

5.4 

1.0 

1.1 

1.1 

1.3 

0.6 

90 
to' 

P.99 

S:S0LVENT PsPOSITIVE NsANTHRAMINE W/O S9 *sTEST COMPOUND 

C0Nsl91482 CHEMsEDC STRAIN:97 DATE:040883 ACTIV: ACTx:05 

R 

E 

V 

E 

R 

T 

A 

N 

T 

C 

0 

u 

N 

T 

2250.0 • 

t 

i 

i 

! 

200C0 • 

; 

i 

! 

; 

1750.0 • 

• 

i 

i 

; 

1500.0 .• 

; 

i 

i 

; 

1250.0 • 

; 

; 

t 

i 

10C0.0 • 

f 

I 

I 

I 

750.0 • 

» 

i 

i 

i 

500.0 • 

; 

i 

i 

i 

250.0 • 

! s 

, i 

t 

0.0 • 

PLOT OF AVGCOUNT'DOSE SYMBOL IS VALUE OF SPIND 

10 100 1300 

DOSE IN MICROGRAMS 

91 
P.1 

.W 

S=S0LVENT P=P0SITIVE N=ANTHRAMINE W/0 S9 *=TEST COMPOUND 

C0N=191482 CHEM=EDC STRAIN=97 DATE=0408e3 ACT1V=CSD001 ACTX=C5 

R 

r 

V 

r 

T 

A 

N 

T 

C 

0 

u 

N 

T 

1800.0 • 

• 

; 

i 

! 

• 

f 

! 

; 

• 

• 

t 

t 

i 

• 

• 

i 

• 

; 

i 

• 

i 

; 

i 

i 

• 

• 

i 

; 

i 

• 

t 

; 

t 

i 

• 

i 

t 

t 

i 

1600.0 

1400.0 

1200.0 

1000.0 

f 00.0 

600.0 

4c: 

200.0 

0.0 

PLOT OF AVGCOUNT.OOSE SYMBOL IS VALUE OF SPIND 

IC ICC 10C C i :oc 

DCSE IN "UROGRAM S 
P.2 

f '••• 

S=SCLVEN T P=POSITIV E N=A"NTHRAMIN E W/0 S9 • = TES T COMPOUND 

C0N=19H8 2 CHEM^DC STRAIN=97 DATE=040fe83 ACTJVrHCPAOl ACTX=0^ 

PLOT OF AVGCOUNT«DOSE SYMBOL IS VALUE OF SPIND 

! 

t 

1100. 0 i» 

! 

! 

; 

1000. 0 • 

! 

I 

! 

900.0 • 

| 

! 

! 

800.0 • 

! 

! 

i 

700. 0 • 

! 

! 

600. 0 

500. 0 

400. 0 

300. 0 

200.G 

100. 0 

0 . 0 

10 100 

DOSE IN MICROGRAM S 

1C00 1 CO 

93 
P.3 

># MUTAGENICITY OF EDC - TA98 

MUTAGENICIT Y 

EXP DATE: 040883 CHEMICAL: EDC 

TEST TYPE: 05 STRAIN: TA98 

DILUTION: : PLATED: 

MIXTURE: TECHNICIAN: LGM 

CONTRAC T NO. : 19146 2 

ACTIVATION : 

PATCH : 0073 

COLONIES: 

SOLVENT/ 

POSITIVE 

SI 99 

PI 03 

P2 03 

DOSE 

LEVEL 

0.00 

1.C0 

2.00 

50.00 

IOC.CO 

25C.00 

SOC.00 

1C0C.00 

PLATE 

COUNT 

27 

161 

264 

40 

44 

44 

52 

37 

A: 

BG 

PLATE B : 

COUNT BG 

41 

163 

265 

4 

30 

34 

40 

26 

PLATE C 

COUNi" BG 

31 

141 

424 

30 

32 

45 

29 

25 

AVERAGE 

COUNT 

33.0 

155.0 

317.7 

35.0 

35.3 

41.0 

40.3 

29.3 

STD DEV 

CCUNT 

7.2 

12.2 

92.1 

7.1 

7.6 

6.1 

11.5 

f .7 

O/C MR ME 

1.0 

4.7 

9.6 

1.1 

1.1 

1.2 

1.2 

0.9 

MUTAGENICIT Y 

E X P DATE : 04oee3 CHEMICAL : ED C 

TEST TYPE: 05 STRAIN: TA98 

DILUTION: U 20 PLATED: ICO 

TECHNICIAN: LG M MIXTURE: 5CQ 

CONTRACT NO 

ACTIVATION: 

BATCH: D073 

C'OLOMES = 

: i°i«8 2 

CSD001 

SOLVENT/ 

POSITIVE 

SI 

PI 

P2 

99 

04 

04 

DOSE 

LEVEL 

0.00 

1.00 

2.00 

50.00 

100.00 

250.CO 

500.00 

1CGG.C0 

PLAT E A : 

COUNT EG 

3

C 

915 

1839 

32 

22 

49 

39 

49 

PLATE B : 

COUNT BG 

42 

803 

l

c 48 

36 

47 

33 

35 

65 

PLATE C: 

COUNT PG 

42 

990 

2020 

44 

52 

36 

42 

41 

AVERAGE 

COUNT 

41.C 

902.7 

1935.7 

37.3 

40.3 

39.3 

38.7 

51.7 

STC DEV 

COUNT 

1.7 

c

«».l 

91. 1 

6 . 1 

16. 1 

8 . 5 

3 . 5 

12.2 

0 /c 

'« 

u 

1 

MR 

1.3 

22.0 

47.2 

0.9 

1.0 

1.0 

0.° 

1.3 

Mf. 

M U T A G 

EXP CATE : 

TEST TYPE 

DILUTION: 

E N I C 

C40883 

05 

l: 20 

V.IXTUFE: 500 

I T Y 

CHEMICAL : EDC 

STRAIN: TA98 

PLATED: ICO 

TECHNICIAN: LGM 

CONTRAC T VO. : 191*8 2 

ACTIVATION: HEPACI 

BATCH : D073 

COLONIES: 

SOLVENT/ 

POSITIVE 

SI 

PI 

P2 

99 

04 

C4 

COSE 

LEVEL 

0.00 

0.50 

1.C0 

50.CO 

10C.CC 

250.00 

500.00 

1000.CO 

PLATE 

COUNT 

40 

216 

1046 

54 

40 

40 

5C 

43 

A : 

BG 

PL&T E P : 

COUNT PG 

42 

2e8 

986 

42 

39 

39 

43 

30 

PLAT E c: 

COUNT PG 

4C 

2 35 

1107 

46 

41 

42 

52 

34 

AVERAGE 

COUNT 

40. 7 

246. 3 

10*6. 3 

47. 3 

40. 0 

40. 3 

46. 3 

35. 7 

STC rr.v 

CCUM 

1.2 

27. * 

60. 5 

6 . 1 

l . C 

l » b 

4 . 7 

6 . 7 

C/r MR 

1.0 

6 . 1 

25. 7 

1.2 

1.0 

1.0 

1.2 

vr. 

94 
P.4 

I S:sOLVENT P:P0SITIVE N=«NTHRAMINE W/0 S9 »s?E$1 COMPOUN D 

C0N:19148 2 

! 

! 

330.0 • 

I 

! 

| 

300. 0 * 

| 

! 

! 

270. 0 • 

! 

! 

240. 0 

210. 0 

160. 0 

150.C 

12C.C 

90. 0 

6 0 . C 

30. 0 

0 . 0 

CHEM=EDC STRAIN: Q 8 

PLOT OF AVGCOUNT.OOSE 

DATL":040883 ACTIV: ACT*=05 

SYMBOL IS VALUE OF SPIND 

10 ICC 

DCSE IN MICROGRAMS 

&CC-0 i rec 
P.5 

!«->" 

( » 

S=SOLVENT PrPOSlTIVE N=ANTHRAMINE U/O S9 *=TEiT COMPOUND 

C0N=191482 CHEM=EDC STRAIN=98 DATE=040883 ACTIV=CSD001 ACT*=C5 

PLOT OF AyGCOUNT*DOSE SYMBOL IS VALUE OF SPIND 

F. 

E 

V 

E 

P 

T 

A 

N 

T 

C 

0 

u 

N 

T 

2250.0 • 

2000.0 • 

1750.0 • 

i. 

i 

! 

i 

1500.0 4-

1250.0 • 

10CC.C 

750.0 • 

500.0 • 

250.0 « 

0.0 • 

10 100 1C00 

DOSE IN MICROGRAMS 

1 0 C 0 C 

96 
P.6 

rr 

-ym-t- .utjuw— <ipi^i^p.".jw*«^i * • l.iiliniMti " II.;IJ.^^-' F fcwn ••^-^•m.yy^^gJP»-.»i|gifWiM»y^^)BB»^|W)l^ty>qy^^|^i .rBHiun^i ^ m«,jij i w^jw^ | 

\ & ' 

S=SOLVENT P=PCSITIVE N=ANTHRAMINE W/0 S9 «=TEST COMPOUND 

C0N=191482 CHEM=EDC STRAIN=9fi DATE=040883 ACTIV=HEFA01 ACTXr 0 5 

PLOT OF AVGCOUNT.DCSE SYMBOL IS VALUE OF SFIND 

; 

i 

1100. 0 • 

1000. 0 • 

900. 0 • 

R 

E 

V 

E 

R 

T 

A 

N 

T 

C 

0 

U 

r! 

T 

800 . 0 

700. 0 

600. 0 

500. 0 

400 . C 

300. 0 

• 

t 

j 

• 

• 

• 

• 

• 

• 

i 

i 

i 

• 

; 

t 

• 

200. 0 • 

100. 0 • 

0 . 0 • 

10 ICC 

DOSE IN MICROGRAMS 

10C 0 lfO O 

97 
P.7 

gn niml.lpa-U.jl ',j.»ji!i »«i.ui«im.p»_u-.11 'vmnwwii^nmefpemma^m^mf iwnww.uw m-y uu m-^w jiti*w»w ,i,ui 

MUTAGENICITY OF EDC - TA100 

MUTAGENI C 

EXP DATE*. C40883 

TEST TYPE: 05 

DILUTION: : 

MIXTURE: 

SOLVENT/ DOSE 

POSITIVE LEVEL 

T Y 

CHEMICAL : EDC 

STRAIN : TA100 

PLATED: 

TECHNICIAN: LGM 

SI 99 0.00 

PI 01 1.C0 

P2 01 2.00 

50.00 

100.00 

250.00 

500.00 

1000.03 

MUTAGENI C 

EXP DATE: 040883 

TEST TYPE : C5 

DILUTION: l: 20 

MIXTURE: 500 

PLATE 

COUNT 

339 

603 

7B3 

371 

284 

351 

344 

572 

A: 

86 

PLATE B: 

COUNT BG 

320 

598 

1023 

343 

T20 

350 

327 

I T Y 

CHEMICAL : EDC 

STRAIN : TAIO O 

PLATED: 100 

TECHNICIAN: LGM 

CONTRAC T NO.: 

ACTIVATION : 

BATCH : 0073 

COLONIES: 

191462 

PLATE 

COUNT 

248 

568 

909 

301 

192 

344 

323 

561 

c: 

eG 

AVERAGE 

COUNT 

302.3 

589.7 

935.0 

338.3 

232.0 

348.3 

331.3 

589.7 

STO DEV 

COUNT 

48.0 

18.9 

120.0 

35.2 

47.2 

3.8 

11.2 

40.5 

CONTRAC T NO. 

ACTIVATION : 

BATCH : DC73 

COLONIES: 

: 191462 

CSD001 

Q/C MR 

NA 

A 

A 

1.0 

2.0 

3.0 

1.1 

0.8 

1.2 

1.1 

2.0 

MEC 

SOLV'NT/ 

FOSITIVE 

SI 

FI 

P2 

99 

04 

04 

DOSE 

LEVEL 

0.00 

1.00 

2. 00 

50.00 

100.00 

250.00 

503.00 

1C00.00 

PLATE 

COUNT 

266 

965 

2193 

236 

352 

212 

230 

202 

A: 

PG 

PLAT E e: 

COUNT RG 

256 

1522 

2622 

293 

311 

230 

202 

241 

PLATE C: 

COUNT 86 

245 

1507 

2515 

194 

339 

221 

161 

220 

AVERAGE 

COUNT 

255.7 

1332.7 

2443.3 

241.0 

334.C 

221.0 

197.7 

221.0 

STD DEV 

COUNT 

10. 5 

315.C 

223. 3 

49. 7 

21. 0 

9 . 0 

34. 7 

1<».5 

fi/C MR MCC 

NA 

A 

A 

1.0 

5 . 2 

9 . 6 

0 . 9 

1.3 

0.? 

O.P 

0 . 9 

U N I C I T Y 

E XP CATE : C4088 3 

T!IST TYPE : 05 

DILUTION : 1: 20 

MIXTURE: 500 

CHEMICAL : IOC 

STRAIN : TAIOO 

PLATED: 100 

TECHNICIAN: LGM 

CGNTRACT NO. 

ACTIVATION : 

BATCH: P073 

COLONIES: 

: 1914 82 

HEPAC1 

SOLVENT/ 

POSITIVE 

SI 

PI 

P2 

99 

C4 

04 

DOSE 

LCVEL 

O.CO 

0.50 

1.00 

50.00 

100.00 

250.00 

5 0 0 . C 0 

1000.0 0 

PLATE 

COUNT 

272 

554 

1422 

2*4 

26b 

263 

2PE 

262 

A: 

SG 

PLATE «: 

COUNT P.G 

213 

603 

1617 

282 

306 

256 

343 

194 

FLATE C: 

COUNT PG 

275 

571 

1467 

274 

261 

35(> 

297 

163 

AVERAGE 

COUNT 

253.3 

576.0 

15 0 2.0 

r G o. c 

277.7 

292.3 

309.3 

213.0 

STD DEV 

CCUNT 

35. P 

24.9 

102.1 

5.3 

24.7 

55.2 

29.5 

61.7 

G/C 

NA 

A 

MR 'EC 

1.0 

j 

o 

9S 
P.8 

UJW^PcHHWBH • JIPU.I •i»yiMiti!I.Ll •*tA.jpi»WM'\WM^JF''l»iT* "IHWPH. i . .»« ipBUfii i 

1 

-1 

S:SOLVENT P:P0SITIVE NZANTHRAMIN' E W/C S9 *:TEST COMPOUND 

C0N=191462 CHEM:EDC STRAIN=100 CATE:Q40863 ACTIV: ACTX-Q5 

PLOT OF AVGCOUNT.DOSE SYMBOL IS VALUE OF SPIND 

900. 0 • 

! 

! 

i 

i 

600.0 * 

! 

! 

! 

i 

700. 0 • 

! 

i 

i 

! 

600. 0 • 

i 

i 

! 

i 

500. 0 • 

• 

! 

400. 0 

300. 0 • 5 

200.C 

100. 0 • 

! 

0 . 0 • 

io mo 

COSE IN -ICROGRA- S 

1 0 0 0 i :oc o 

99 
P.9 

pi; IJUHff ^HJ*m'aw^''J ^ iflBJ, .; •^jgMjjiiippRpjj 

i 

R 

r 

V 

E 

R 

T 

A 

N 

T 

C 

0 

u 

N 

T 

S

=S0LVENT P:P0SITIVE N:ANTHRAMINE U/0 S9 «:TEST COMPOUND 

C0N:191482 CHEM=EDC STRAINMOO DATE=040883 ACTIV = CSD001 A'CT* = 05 

PLOT OF AVGCOUNT.OOSE SYMBOL IS VALUE OF SPIND 

; 

2750.0 • 

i 

; 

t 

2500.0 • 

! P 

! 

2250.0 • 

2000.0 

1750.0 

1500.0 • 

1250.0 

1 C GO.0 • 

750.C • 

500.0 • 

250.0 • 

0.0 • 

10 ICO 

COSE IN MICROGRAM? 

1000 1 COCO 

100 
P.10 

S=SOLVENT P:POSITIVE N:ANTHRAMINE W/0 S9 *=TEST COMPOUND 

C0N=191482 CHEM:EDC STRAIN:100 DATE=040883 ACTIV:HEPA0 1 ACTX:05 

PLOT OF AVGCOUNT.OOSE SYMBOL IS VALUE OF SPIND 

R 

E 

V 

r 

R 

T 

A 

N 

T 

C 

0 

u 

N 

T 

1500.0 • 

i 

• 

1400.0 • 

• 

i 

1300.0 • 

i 

! 

1200.0 • 

• 

i 

1100. 0 • 

I 

I 

1000. 0 • 

I 

I 

900.0 • 

i 

i 

eoo.o • 

i 

i 

700.0 • 

! 

i 

6 00.0 • 

; 

• 

500.0 • 

; 

i 

400. 0 • 

I 

I 

300.0 • 

' S 

i 

200.0 • 

i 

• 

100.0 • 

I 

I 

0.0 -» ! 

0 10 IOC 

DOSE IN MICROGRAM S 

1 CO 0 1 i, J 0 c 
P.11 

.V ' MUTAGENICITY OF EC - TA97 

y> 

| 

MUTAGENICIT Y CONTRACT NO.: 191482 

EXP DATE.* 040883 CHEMICAL: EC ACTIVATION: 

TEST TYPE: 05 STRAIN: TA97 BATCH! D073 

DILUTION: : PLATED: COLONIES: 

MIXTURE: TECHNICIAN: LGM 

SOLVENT/ DOSE PLATE A: PLATE B: PLATE C: AVERAGE STD DEV G/C MR MEC 

POSITIVE LEVEL COUNT BG COUNT BG COUNT B6 COUNT COUNT 

SI 99 0.00 188 193 194 191.7 3.2 A l.C 

PI 02 50.00 735 832 664 743.7 84.3 A 3.9 

P2 02 100.00 1875 2285 2249 2136.3 227.0 A 11.1 

33.00 258 260 245 254.3 8.1 1.3 

100.00 321 337 291 316.3 23.4 1.6 

333.00 513 475 540 509.3 32.7 2.7 

1C00.00 866 922 972 920.0 53.0 i 4.8 * 

3333.00 1145 1101 1124 1123.3 22.0 5.9 

MUTAGENICIT Y CONTRACT NO.: 191482 

EXP DATE: 040883 CHEMICAL: EC ACTIVATION: CSD001 

TEST TYPE: 05 STRAIN: TA97 BATCH: D073 

DILUTION: i: 20 PLATED: 100 COLONIES: 

MIXTURE: 500 TECHNICIAN: LGM 

SOLVENT/ DOSE PLATE A: PLATE B: PLATE*C: AVERAGE STD DEV G/C MR MEC 

POSITIVE LEVEL COUNT BG COUNT BG COUNT BG COUNT COUNT 

SI 99 0.00 228 215 225 222.7 6.8 NA 1.0 

FI C4 1.00 678 703 696 633.0 13.2 A 3.1 

?2 04 2.00 1713 1808 1873 1798.0 80.5 A 8.1 

33.00 188 243 212 214.3 27.6 1.0 

100.00 220 256 303 258.7 40.1 1.2 

333.00 455 572 431 486.0 75.4 2.2 

1000.CO 912 720 955 862.3 125.1 ; 3.9 • 

3333.CO 822 704 547 691.0 L38.0 3.1 

MUTAGENICIT Y CONTRACT NO. : 1?14? 2 

EXP DATE: 040883 CHEMICAL: EC ACTIVATION: HEPA01 

TEST TYPE: 05 STRAIN: TA97 BATCH: C073 

DILUTION: K 20 PLATED= 100 COLONIES: 

MIXTURE: 500 TECHNICIAN: LGK , 

SOLVENT/ DOSE PLATE A: PLATE P : PLATE C: AVERAGE STD DEV G/C

 MP MEC 

POSITIVE LEVEL COUNT PG COUNT eG COUNT PG COUNT CCUNT 

SI 99 0.00 242 199 161 207.3 31.3 NA ' 1.0 

PI 04 0.50 378 344 35° 3b0.3 17.0 NA 1.7 

P2 04 1.G0 1135 1147 1099 1127.0 25.0 A 5.4 

33.00 215 192 222 239.7 15.7 i 1.0 

IOC.00 205 255 242' 234.0 25.9 1.1 

333.0 0 339 299 301 313. 0 22.5 1.5 

1000.0 0 824 824 612 820. 0 6. 9 4. 0 • 

3333.0 0 1308 1186 1260 1252.C 60. 4

 ; b.C 

102 
P.12 

ip 

S=SOLVENT P:POSITIVE N=ANTHRAMINE U/0 S9 *=TEST COMPOUND 

C0N=191462 CHEM:EC STRAIN:S7 DATE:04C883 ACTIV: ACTX=05 

PLOT OF AVGCOUNT^DOSE SYMBOL IS VALUE OF SFINp 

2250.0 • 

2000.0 • 

1750.0 • 

! 

! 

! 

1500.0 

1250.0 

! 

1000.0 • 

750.0 

500.C 

250.n 

0.0 • 

1C irc o l rice 

CCSE If. "IC^CGRA^S 

103 
P.13 

i S:SOLVENT P:P0SITIVE N:ANTHRAMINE W/0 S9 *:TEST COMPOUND 

C0N:191482 CHEM:EC STRAIN=97 DATE:0408b3 ACTIV=CSD0C1 ACT*=05 

PLOT OF AVGCOUNT.DOSE SYMBOL IS VALUE OF SPIND 

1800.0 • 

i 

t 

i 

1600.0 • 

R 

Z 

V 

E 

R 

T 

A 

N 

T 

C 

0 

u 

% 

T 

1400.0 

1200.0 

1000.0 

800. C 

630. 0 

• 

! 

• 

• 

i 

! 

i 

. 

• 

i 

f 

t 

t 

• 

400.C • 

200.C « 

i 

• 

T 

i 

0.0 • 

1 0 1ZZ 

:?SE IN MICRCGR Af.S 

K O O 1CC01 
P.14 

i'~ 

A 

I 

» 

R 

E 

V 

r 

R 

T 

A 

N 

T 

C 

0 

U 

\ 

T 

CON:19 

1500.0 

1400.0 

1300.0 

1200.0 

1100.0 

1330.0 

900.0 

600.C 

700.3 

600.0 

500.0 

«C0.3 

300. 0 

200. C 

100.0 

0.0 

S:SOLVENT P:PCSlTIVE N:ANTHRAMINE W/0 S9 «:TEST CGMPGUND 

1482 CHEM = EC STRAIN:97 " DATE:C40683 ACT1V:HEPA01 ACTX=05 

PLOT OF AVGCOUNT.OOSE SYMBOL IS VALUE OF SPIND 

i 

« , 

! 

• 

• 

i 

! • 

• 

• 

! 

• P 

• 

• 

! 

« ' • 

i 

i 

• * 

• 

• 

• 

! 

• 

. 

i 

i 

. 

• 

i 

. ; 

1 

1 

• 

! P 

• ! 

• . 

• • 

• S « 

t ' ' 

. 

i 

t 

l 

' 0 1 10 100 1 C 0 3 ' 1. CCO 

OCSE IN MICROGRAMS 

105 

1 
P.15 

#• 

! 

M U T A G 

EXP DATE: 

TEST TYPE 

DILUTION: 

MIXTURE: 

SOLVENT/ 

POSITIVE 

SI 99 

PI C3 

P2 03 

MUTAGENICITY OF EC - TA98 

E N I C I T Y CONTRACT NO.: 191482 

0436e3 CHEMICAL : EC ACTIVATION : 

: 05 STRAIN : TA9 8 EATCH : 0073 

: PLATEE= COLONIES: 

TECHNICIAN: LCM 

DOSE PLATE A: PLATE B: PLATE C: AVERAGE STD DEV Q/C MR MEC 

LEVEL COUNT BG COUNT BG COUNT BG COUNT COUNT 

0.00 27 41 31 33.0 7.2 A 1.0 

1.00 161 163 141 155.G 12.2 A 4.7 

2.00 264 265 424 317.7 92.1 A 9.6 

33.00 3b 26 28 30.7 4,6 0.9 

100.CO 28 26 30 28.0 2.0 0.8 

333.00 40 36 41 39.7 1.5 1.2 

1C00.C0 33 52 45 43.3 9.6 1.3 

3333.00 6G 42 64 55.3 11.7 1.7 

MUTAGENICIT Y CONTRACT NO. : 191462 

EXP CATE: 040883 CHEMICAL: EC ACTIVATION: CS0001 

TEST TYPE: 05 STRAIN: TA98 BATCH: 0073 

DILUTION: i: 20 PLATED: 100 COLONIES: , 

MIXTURE: 500 TECHNICIAN: LGM i 

SOLVENT/ OOSC PLATE A: PLATE B: PLATE C: AVERAGC STD DEV Q/C MR "EC 

POSITIVE LEVEL COUNT EG COUNT 8G COUNT PG COUNT CCUNT 

SI 99 0.00 39 42 42 41.0 1.7 NA 1.0 

PI C4 1.CO 915 803 990 902.7 94.1 A 22.C 

P2 C4 2.CO 1839 1948 202C 1935.7 91.1 A 47.2 

33.CO 41 44 37 40.7 3.5 ! 1.3 

ICO.CO 44 50 44 4fc.O 3.5 1.1 

333.CC 45 46 50 47. Z 2.6 1.1 ' 

ICOO.OC 57 65 67 63.0 5.3 1.5 

3333.00 62 ei 73 72.0 9.5 l.e 

MUTAGENICIT Y CCNTRACT NO. : 191*82 

EXP Ltvz: r*C5E3 CHEMICAL : EC ACTIVATION : HEPAP I 

TEST TYPE:

 ; 05 STRAIV: TA98 PATCH: CC73 

DILUTION: i: 20 PLATEC: ICC CCLCMES = 

MIXTURE: 500 TECHNICIAN: LGM 

SOLVTNT/ COSE PLATE A: PLATE F: FLATE C: AVERAGE STC PEV G/C MR MEC 

POSITIVE LEVEL CCUNT BG COUNT BG COUNT BG COUNT COUNT 

i 

SI 99 0.00 40 42 40 40.7 1.2 NA 1.0 

FI 0* 0.50 216 288 235 246.3 37.3 A 6.1 

P2 04 1.0C 1C46 986 1107 1346.3 60.5 A 25.7 

33.00 28 52 44 41.3 12.2 1.0 

100.CO 46 49 43 *b.C 3.C 1.1 

333.CO 59 51 49 53.0 5.3 1.3 

1 COO.CO 76 71 53 68.3 9.3 1.7 

3333.CO 89 85 64 86.0 2.6 2.1 

106 
r 

P.16 

L* 

S:SOLVENT P:P0SITIVE N: AN*T HR AM INE W/0 S9 «:TEST COMPOUND , 

C0N:191462 CHEM:EC STRAIN:96 DATE:040883 ACTIV: ACTX:05 

PLOT OF AVGCOUNT.DCSE SYMBOL IS VALUE OF SPIND 

330.0 • 

j 

i 

R 

E 

V 

E 

R 

T 

A 

U 

T 

C 

0 

u 

N 

T 

300.0 • 

! 

• 

i 

270.0 • 

t 

• 

! 

240.0 • 

• 

; 

; 

210.0 • 

• 

i 

! 

lec.o • 

150.C • 

120.0 • 

90.0 • 

60. 

30.0 • S 

0.0 • 

i 0 10 100 1?00 1 CC 0 0 

DOS E IN MICROGRAM S 

10 7 
P.17 

S:S0LVENT P=POSITIVE N:ANTHPAMINE W/0 S9 «:TEST COMPOUND 

C0N=191482 CHEM:EC STRAIN:98 CATE:040883 ACTIV:CSD0C1 ACTX=D5 

PLOT OF AVGCOUNT.OOSE SYMPOL IS VALUE CF SPIND ' 

2250.0 • 

2000.0 • 

! 

1750.0 • 

R 

r 

V 

E 

R 

T 

A 

N 

T 

C 

0 

u 

N 

T 

1500.0 

1250.0 

1000.0 

750.0 

• 

i 

i 

! 

; 

• 

• 

• 

500.0 • 

250.0 • 

0.0 • 

10 ICC 

DOSE IN MICROGRAMS 

1000 1 c C 0 c 

10S 
f.-

P.18 

S=SOLVENT P:P0SITIVE N= ANTHR A* I NE U/0 S9 *:TEST COMPOUND 

CO :191482 CHEM:EC STRAIN:98 DATE:Q40PR3 ACTIV:HEPA01 ACT»=05 

PLOT OF AVGCOUNT.OOSE SYMPOL IS VALUE OF SPINO 

» 

i 

1100.0 • 

! 

• 

! 

1000.0 • 

! 

i 

! 

900.0 • 

! 

i 

i 

800.0 • 

! 

i 

! 

700.0 • 

630.0 • 

500.0 

400.0 

300.0 • 

200.0 

100.0 

0.0 

10 ICC 1CCC i :cti 

CCSE IN ^ICRrGRt^S 

ir.o 
tr 

P.19 

MUTAGENICITY CF EC - TAlOO 

M U T A G E N I 

EXP DATE: 040883 

TEST TYPE: 05 

OILUTION : I 

MIXTURE: 

SOLVENT/ DOSE 

POSITIVE LEVEL 

SI 99 0.00 

PI 01 1.00 

PZ 01 2.00 

33.00 

100.00 

333.00 

1 ICG.00 

3333.00 

CIT Y 

CHEMICAL : EC 

STRAIN 

PLATET 

; TAlOO 

TECHNICIAN: LGM 

PLATE AI 

COUNT BG 

339 

603 

783 

612 

834 

1199 

2258 

2200 

PLATE B: 

COUNT eG 

320 

598 

1023 

444 

679 

1492 

2414 

2212 

CONTRACT NO.: 

ACTIVATION : 

BATCH : DC73 

COLONIES: 

PLATE C: AVERAGE 

COUNT BG COUNT 

248 302.3 

568 589.7 

909 905.0 

445 500.3 

885 866.0 

1412 1367.7 

2365 2345.7 

2162 2191.3 

191462 

STD DEV 

COUNT 

48.0 

18.9 

120.0 

96.7 

27.9 

151.4 

79.e 

26.1 

Q/C MR 

NA 1.0 

A 2.0 

A 3.0 

1.7 

2.« 

4.5 

7.8 

7.2 

MEC 

MUTAGENI C 

ZVP DATE: C4C88 3 

TEST TYPE : C5 

DILUTION : I I 2 0 

MIXTURE : 50C 

T Y 

CHEMICAL: EC 

STRAIN : TA1C0 

PLATED: 100 

TECHNICIAN: LGM 

CONTRAC T NO. : 19148 2 

ACTIVATION : CSDOO I 

BATCH : D073 

COLONIES: 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE A: 

COUNT BG 

PLAT E P: 

COUNT PG 

PLATE C: 

CCUNT PG 

AVERAGE 

COUNT 

STD DEV 

COUNT 

G/C MR MEC 

SI 

PI 

P2 

99 

04 

C4 

C O O 

1.00 

2.CC 

33.00 

1C0.C0 

333.CO 

1C0C.30 

3333.00 

266 

969 

21R3 

256 

448 

1210 

2244 

2415 

256 

1522 

2622 

281 

351 

1C08 

1567 

2262 

245 

1507 

2515 

255 

369 

666 

2016 

2289 

255.7 

133 2.7 

2443.3 

264.7 

389.3 

961.3 

2042.3 

2322.0 

10.5 

315.0 

223.3 

14.2 

51.6 

275.0 

189.9 

bl.7 

NA 

A 

A 

1.0 

5.2 

9.6 

1.0 

1.5 

3.8 

8.0 

9.1 

M U T A 3 

EXP DATE : 

TTST TYP : 

DILUTION : 

:E N I C 

040883 

: 05 

i: 20 

WIXTU=?E: 5CC 

I T Y 

CHEMICAL : EC 

STRAIN : TAIO O 

PLATEQs 1C0 

TECHNICIAN: LGM 

CONTRAC T NO.'. 19148 2 

ACTIVATION : HEPAC I 

BATCH : DC73 

COLONIES: 

SOLVENT/ 

POSiTivr 

DOSE 

LEVEL 

PLATE A: 

COUNT BG 

PLATE 

CCUNT 

e: 

BG 

PLATE C: 

COUNT PG 

AVERAGE 

COUNT 

S T D DE V 

COUN T 

Q/C MR KEC 

S I 

P I 

P2 

99 

04 

04 

COO 

C.5 0 

1 . CO 

33.0 0 

100.0 0 

333.0 0 

1 CCC.CO 

3333.0 0 

2 7 2 

554 

142 2 

18? 

3 53 

514 

241 6 

2«-92 

2 1 3 

6 0 3 

lbl 7 

201 

3 9 7 

7 9 7 

146 0 

241 7 

2 7 5 

5 7 1 

146 7 

1S8 

3 3 3 

5 0 6 

101 5 

241 2 

253. 3 

576. 0 

1502. 0 

160. 3 

361. C 

605. 7 

1630. 3 

24<«C.3 

3? . 0 

24. 9 

102. 1 

21 . 5 

32. 7 

165. 7 

715. 9 

44. 8 

NA 

A 

A 

1 . 0 

2 . 3 

5 . Q 

0 . 7 

1 . 4 

2 . ' 

6 . 4 

9 . 6 

110 
P.20 

._ . ,.- ii-. | . . - i . . "•*—~**^~* mmtirnwji|mni.am,iw.uujjmji• 

r 

ft' 

S=S0LVENT P:POSlTIVE N:ANTHRAMINE W/0 S9 *=TEST COMPOUND | 

C0N:191482 CHEM:EC STRAIN:1C0 DATE:040883 ACTIV: . ACTX=05 

• 

i 

2750.0 • 

i 

i 

f 

2500.0 • 

; 

! 

I 

2250.0 • 

; 

• 

• 

2000.3 . 

i 

R ! 

E ! 

V 1750.0 • 

E ! 

R ! 

T ! 

A 1500.0 • 

N • 

T ! 

i 

C 1250.0 • 

0 ! 

U ! 

N ! 

T 10C0.0 • ; 

i 

i 

i 

750.0 • 

i 

i 

i 

500.0 • 

i 

i 

! S 

250.0 • 

i 

t 

i 

0.0 • 

PLOT OF AVGCOUNT.OOSE SYMBOL IS VALUE CF SPIND 

.---•____-- - — - - — -•-----•.- » — • - — •»»- - — --_---_• . 

10 1C0 1C00 1cooc 

DOSE IN MICROGRAMS 

111 
P.21 

JH WUMHWWW^tJJ.MIgUM JXIUJHWWPIWM)^, i J"- <H|WI'.JJiiiyLliJUJW WI^Jipj>US"'Wgr» 

i' ... 

R 

E 

V 

E 

P. 

T 

A 

N 

T 

C 

0 

u 

N 

T 

S=S0LVENT P:P0SITIVE N:ANTHRAMINE W/0 S9 *=TEST COMPOUNO 

C0N:191482 CHEM:EC STRAIN:100 DATE:043883 ACT I V=CSDOO1 ACTt^uS 

PLOT OF AVGCOUNT.DOSE SYMBOL IS VALUE OF SFIND \ 

i 

i 

2750.0 • 

! 

! 

2500.0 • 

2250.0 • 

2000.0 • 

1750.0 • 

1500.0 • 

1250.0 • 

1000.0 • 

750.0 • 

500.0 • 

250.0 • S 

0.0 • 

10 1C0 

DOSE IN MICROGRAMS 

1C00 i : o 3 o 

n: 
P.22 

pw,-i|MJP l iH-.M* I •^IPiPMW.^JWP'JUWj^WlMMUW^^ 

f S=SOLVENT P:P0SITIVE N:ANTHRAMJN E U/0 S9 *=TEST COMPOUND 

C0N:191482 CHEM:EC STRAIS=100 DATE=040683 ACTIV:HEPAO 1 ACTXrQ5 

PLOT OF AVGCOUNT.OOSE SYMBOL IS VALUE OF SPIND 

R 

E 

V 

E 

R 

T 

A 

N 

T 

C 

0 

u 

\ 

T 

2750.C 

2500.0 

2250.0 

2000.0 

1750.0 

15CC.0 

1250.0 

1300.C 

750.0 

50C.C 

250.C 

0.0 

i 

t 

» 

• 

! 

| 

f 

• 

i 

t 

| 

• 

• 

t 

« 

i 

t 

i 

• 

i 

i 

! 

4 

1 

! 

i 

« 

i 

i 

i 

• 

i 

i 

i 

• 

| 

• S 

! 

i 

I 

• 

10 IOC 

CCSE IN MICROGRAMS 

1CC0 

X 113 
i 

P.23 

MUTAGENICITY CF NP TA97 

1 U T A G 

EXF DATE : 

TEST TYPE : 

DILUTIO N 

MIXTURE: 

E N I C I T Y 

:42be3 CHEMICAL : N P 

; 05 STRAIN : TA97 

: PLATED: 

TECHNICIAN: Lr.H 

CONTRAC T VO. : 

ACTIVATION : 

BATCH : 0273 

COLONIES: 

191482 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE 

COUNT 

A : 

BG 

PLATE 

CCU'.T 

t : PLATE 

COUNT 

c: 

BG 

AVERAGE 

COUNT 

STD DEV 

COUNT 

G/C MR MEC 

SI 

PI 

P2 

99 

02 

02 

o.oc 

50. 00 

1C0.CC 

33. CO 

IOC.CO 

333.03 

1 0 G 0 . 0 C 

3333.CC 

132 

562 

1222 

142 

12S 

96 

120 

96 

124 

473 

851 

113 

12 3 

117 

96 

125 

115 

346 

1393 

98 

122 

95 

13& 

1C6 

123.7 

463.3 

1155.3 

117.7 

123.3 

1C2.7 

116. C 

139.0 

8.5 

108.6 

277.1 

22.4 

4.2 

12.4 

21.1 

14.7 

1.0 

3.7 

9.3 

1.0 

1.0 

O.fl 

l.C 

0.«* 

MUTAGENICIT Y 

E X P DATE : C42st3 

TES T TYPE : J5 

DILUTION: l: 20 

M1»TURE= 5."3 

CHEMICAL : N P 

STRAIN : TA97 

PLATED: ICC 

TECHNICIAN: Lt M 

CONTAC T NO.: 1914£2 

ACTIVATION: CSD0C2 

BATCH: D273 

COLOME S = 

SOLVENT/ 

POSITIVE 

COSE 

LEVEL 

PLATE 

COUNT 

A : 

to 

PLAT E e: 

COUf.T r. o 

PLATE 

COUNT 

c: 

BC 

AVERAGE 

COUNT 

STD DEV 

CCUNT 

G/C MR MEC 

SI 

FI 

P2 

99 

C4 

C4 

C C C 

l.CC 

2. JS 

33. CO 

IOO-.DC 

333.:o 

»*•-,• • -. -. 

1 . w W • «. * 

3333.00 

134 

595 

106* 

155 

lft 

13 = 

I'D 

136 

124 

0 = 9 

1257 

145 

150 

133 

146 

144 

129 

716 

114« 

144 

lit 

147 

153 

13? 

12c.O 

671.3 

llbP.C 

145.0 

152. C 

139.3 

16 3.0 

13=.3 

5.3 

63.2 

94.6 

6.1 

35.0 

7.1 

23.6 

4.2 

1.0 

,5.2 

'9.0 

1.1 

1.2 

1.1 

,1.3 

1.1 

M U T A G \; l c I T Y 

EXP GiTE: : <• I c 6 3 

TTST

 T YFE. '.'5 

3ILLs.O-.= l: 20 

MixTuSC= 57C 

CHEMIC-L : N P 

STKAI;< : T A = 7 

PLATED: IOC 

TECHNICIA N L-i 

CONTRACT NC.: 19l4b2 

ACTIVATION.* HEPAC1 

bATCl-;: D273 

COLONIES: 

SOLVENT/ 

PCS1TIVE 

DOSE 

lEV~L 

=>LAT£ A : 

C3UNT bG 

PLATE r: 

COUNT r.G 

PLATE 

COUNT 

c: 

tr. 

i V E P A G E 

COUN T 

S T C CE V C/ C 

COUNT 

MR MEC 

SI 

Pi 

P2 

C4 

:.4 

G.5C 

1. CCSS.CO 

13 0.03 

333.CO 

i: c o. o o 

3333.C O 

140 

496 

:LI 

149 

163 

2C-3 

151 

17C 

119 

3 6 6 

= 93 

14C 

143 

141 

13* 

156 

151 

523 

lfffc 

lf 0 

1 5 J 

155 

122 

163 

13 6. 7 

465. 3 

K33.C -

156. 3 

161 . ^ 

lfafc.7 

135. 7 

163. C 

16. 3 

7 3. 3 

4f . 3 

2 1 . C 

20. 2 

32. 1 

14. 6 

7 . 0 

l . C 

3 . 4 

7 . 6 

1 . 1 

!l. 2 

1 . 2 

i . : 

1 . 2 

ii.; 
P.24 

S:SOLVENT P:POSITIV E N:ANTHRAMINE y/ 0 S9 *:TES T COMPOUND 

C0N:191«8 2 

R 

r 

V 

R 

T 

A 

N 

T 

«• 

3 

J 

N 

T 

1500.0 

1400.0 

1300.0 

1200.0 

11C0.C 

1000*0 

°DG.C 

eoc-.o 

700.0 

6CC.0 

1 

• 

! 

• 

• 

! 

• 

• 

• 

1 

• 

• 

1 

! 

• 

1 

! 

• 

• 

• 

• 

i 

• 

• 

! 

i 

• 

i 

! 

5C0. 0 • 

400. 0 

3C0. 0 

CHEM:NP STRAIN:9 7 

PLOT CF AVGCOUNT'DOSE 

DATE:C4268 3 ACTIV " ACTV:Q5 

SYMBOL I S VALUE OF SPINO 

2C0. 0 

1C0. 0 

j 

! S 

• 

t 

i 

o . y • 

io iro 

DOSE th MICROGRAMS 

1CC3 I ft » r. 1 

115 
pP.25 

S:SCLVENT P:P0SITIVE N:ANTHPAMINE W/C S9 «:TEST COMPOUND j 

C0N=1°1482 CHEM:NP STRAIN:97 DATE:C428c3 ACTIV:CS0CC2 ACTX:C5 

PLOT OF AVGCOUNT.OOSE SY«5CL IS VALUE OF SPIND 

C 

0 

U 

V 

T 

1500. 0 • 

i 

; 

1400. 0 • 

! 

! 

1300. 0 • 

i 

! 

1200. 0 • 

• 

; 

1100. 0 • 

! 

i 

1030. C • 

i 

i 

900. 0 • 

i 

! 

530.C • 

» 

i 

700. 0 • 

• 

; 

bOC.C • 

5CC.C 

4 0C O • 

30C. 0 

2 0 0. 0 

IOC. 3 

C C • 

10 ICC 

DOSE IN VICRCGPA-S 

i : : 1:0 

116 
IT' 

P.26 

S:SOLVENT P:FOSITIVE N:ANTHRAMJNE «/ 0 S9 «:TEST COMPOUND 

C0N:191482 ChEMrNP STRAIN:97 DATE:C42883 ACTIV:HEPAC1 ACTi=05 

V 

T 

A 

N 

T 

3 

U 

\ 

T 

f 

1100. 3 • 

• 

! 

; 

1000. 0 • 

I 

I 

I 

900. G • 

• 

; 

i 

6C0.0 • 

• 

I 

I 

70C 3 • 

• 

i 

! 

6CCC • 

i 

• 

i 

5 3 C C • 

i 

• 

; 

4 C C • 3 • 

! 

• 

3 wC G * 

i 

; 

i 

2CCC • 

13CC • 

• 

; 

; 

CO • 

PLOT CF AVGCOUNT.OOSE SYMBOL IS VALUE CF SPIND 

13 1 ' C 

DCSE IN MICF.GGFAKS 

xr c c i ?c :c 

117 
MUTAGENICIT Y PF NP - TA9fc 

P.27 

MUTAGE N I C IT Y 

EXP DATE! C42OO 3 

TEST TYPE: 05 

DILUTION : : 

MUTURE = 

CHEMICAL: NP 

STRAIN : TA98 

PLATED: 

TECHNICIAN: LGM 

CONTRAC T NO. : 

ACTIVATION : 

EATCr-: 027 3 

COLONIES : 

19!4fe2 

SOLVENT / 

POSITIV E 

SI 99 

PI 03 

?Z C3 

1 U T A 

E KP CATE 

TEST TYP 

DILUTIO N 

MI*Tue£ = 

DOSE 

LEVE L 

O.US 

l.CQ 

2.CC 

33.C O 

lCu.C C 

333.O C 

1CC0.0 C 

3333.C O 

G E N I C 

: :42ce3 

E: CS 

: I : 20 

500 

PLATE A : 

COUNT BG 

13 

211 

346 

3=» 

3 9 

30 

44 

43 

I T Y 

PLATE 

COUNT 

14 

192 

343 

28 

23 

26 

27 

33 

CHEMICAL: NP 

STRAIN 

PLATED 

: TA98 

= ic: 

TECHNICIAN: L 

fa: 

6G 

CM 

PLATE C: AVERAGE 

COUNT BC CvUl.T 

4 10.3 

171 192.3 

321 336.7 

34 33.7 

3b 32.7 

2P 2e.c 

39 36.7 

24 33.3 

CONTRACT NO.: 

STC CEV 

CCUNT 

5.5 

21.5 

13.7 

5.5 

8.5 

2.0 

8.7 

9.5 

191452 

ACTIVATION: CSD0C2 

eATCr: D273 

COLCNILS: 

6/C 

f. A 

A 

A 

MR 

1.3 

IP.7 

32.7 

3.3 

3.2 

2.7 

3.6 

3.2 

H 

SOLVENT/ DOSE ^LATE A: 

POSITIVE LEVEL COUNT 30 

SI 99 

FIG * 

P2 C* 

0. OC 

l.c: 

2.CC 

33. CC 

lCw.CC 

333.C C 

i: & e •«. c 

3333.CO 

622 

1666 

*»2 

c 5 

w c 

3 5 

33 

4^ 

U T A G 

Ex? DATE : ;<• rJ 

TES T TYPE : Mb 

DILUTION : i: 2 0 

MIATURE = 5:O 

ICIT Y 

PLAT E o: 

COJN T 9 6 

42 

67C 

lbtl 

3R 

37 

37 

33 

45 

PLATE 

COUNT 

42 

662 

1794 

41 

2

C 

35 

3C 

• 9 

CO" 

c: 

BG 

>1t-i 

AVERAGE 

COUNT 

3 £ . C 

fe 5 - . C 

1713. 7 

«r.3 

3

c.O 

35.7 

32.C 

17.7 

CT \c: 

STD DEV 

CCUNT 

6." 

31.7 

7C.7 

2.1 

12.1 

1.2 

1.7 

2.3 

1914' ? 

t/C 

A 

A 

A 

MR 

1. j 

17.3 

45.1 

1.1 

l.C 

3.« 

o.s 

1.3 

MEC 

SOLVENT/ 

=>0S1 T I vE 

SI 

PI 

P2 

99 

C4 

C4 

DOSE 

LEVEL 

C • v c 

C.5C 

1.00 

3 3 . «• « 

IOC.- 0 

3 3 3 . 0 0 

i r c c . c c 

3332.C O 

CHEMICAL: NP 

STRA I tl T0 5 

PLATED - iZZ 

TECHNICIANS LCPLAT E A : 

CCUN T BG 

43 

«C7 

135 1 

<•?• 

* ? 

3fe 

<»2 

34 

PLATE r: 

COUNT vG 

40 

5 7-1 

121 3 

4 0 

bb 

5C 

44 

40 

ACTIVATION : HEFA: I 

fc:!^: DC73 

C t MtS : 

PLATE c : 

COUNT 6G 

3 1 

b C 7 

17««7 

4 0 

37 

49 

4 3 

17 

AVERAG E 

CoUN T 

3R.C 

5 2 c. 3 

143 7.C 

44. 3 

ib. 7 

<•:. : 

43.C 

<.2.3 

STC DEV 

COUNT 

6 . 2 

1 C7.2 

2 7 7.2 

<». C 

11. 0 

7 . 6 

1 . : 

8 . 1 

(./C Ma 

A 1 

A 13 

A 37 

1 

1 

1 

1 

1 

MS! 

118 
S=SCLVCNT P:PCSITIVE N:ANTHRAMINF b/0 S9 «:TEST COMPOUND 

CCN:1514c2 CHEM:NP STR'IN':9S CATE:042883 ACTIV: 4CTi:'C5 

PLOT OF AVGCOUNT*DOSE SYMBOL IS VALUE OF SPIND 

330. 0 

30C 0 

273. 0 

243. C • 

21C . 

U 

\ 

T 

i s 2. : 

i r A « A 

1 - - • V * 

12C D • 

r C C • 

tc. r • 

30. 0 • 

CC • 

10 1*0 

DOSE I N riCRGCPAMS 

IOC 

> • m - 4> m 

1 0 3 I 0 

1.19 
P.29 

r i 

r 

i 

E 

V 

** 

T 

A 

N 

T 

C 

0 

J 

\ 

T 

CGN:i9 

1S0C C 

16C0.0 

1400. 0 

i:oc.c 

1C0C.3 

?0C 0 

•tec ? 

4C0.C 

2 3 C 0 

s= 

148* 

! 

. 

• 

! 

• 

! 

* 

t 

! 

• 

• 

f 

• 

! 

i 

• 

• 

i 

t 

• 

• 

i 

t 

i 

; 

• 

t 

i 

• 

« 

„ | • . , 

SOLVENT P:F0SITIVE N:ANT>RAM I NE W/C SS «=TEST COMPOUND 

CHEM:\P STRAIN:96 DATE:C4:6S3 ACT 1 V=CSDCC2 ACTX=C5 

PLOT OF AVGCOUNT.DCSE SYMBOL IS VALUE OF SPIND 

P 

i 

P 

C O 

i:c 

rOSE I'. MICROGRAMS 

1 •*! **\ 

ic o: l o o: o 
P.30 

r 

f" 

S:SOLVENT P:P0SITIV E N = ANTHRAM INE W/C S9 *=TES T COMPOUND 

C0N:l c14B 2 CHEM=NP STRAlN=9 8 DATE::42S8 3 ACTIV=HEPAC1 ACTX:Q5 

PLOT OF AVGC0UNT-03SE SYMBOL IS VALUE OF SPIN D 

R 

r 

V 

r 

* 

T 

A 

M 

T 

P 

0 

U 

N 

T 

1500. 0 

1400. C 

1300. 0 

1200 . C 

1100.C i 

10CC. 0 

•'OO.G 

n C C . C 

7 C 0 . C 

60C. 0 

5CC. C 

•4C0.C 

30C. 0 

20C . 0 

1C0. 0 

C O 

• 

1 

! 

• 

f 

! 

• 

i 

! 

• 

• 

• 

! 

j 

• 

! 

i 

* • 

» 

; 

• 

i 

» 

• 

; 

t 

• 

! 

; 

• 

t 

» 

• 

i 

• 

; 

; 

• 

i 

i 

* 

t 

! S 

• 

-.•--.--.-.-«---.-.----.--.---•--------- - --4----------^ - .------- - ----. -

1 D ICC 

DOSE I N MICROGRAMS 

IOC o 100 00 

131 

. 
MUTAGTMCITY OF NP - TA1 0 C 

P.31 

MUTAGENICIT Y 

EXP DATE: C429S3 CHEMICAL : NP 

TEST TYPE: 05 STRAIN: TA103 

DILUTION: .* PLATED: 

MIXTURE: TECHNICIAN: LGM 

CONTRACT NO. 

ACTIVATION: 

BtKh l D273 

COLONIES: 

191462 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE A: 

COUNT 3G 

PLATE R: 

COUNT 5G 

PLATE 

COUNT 

c: 

BC 

AVERAGE 

COUNT 

STD DEV 

CCUNT 

C/C MR 

SI 

PI 

P2 

99 

01 

Cl 

0.00 

1.00 

2.00 

33.00 

100.30 

333.00 

i :• o c o c 

3333.00 

114 

355 

920 

177 

158 

150 

119 

143 

113 

2P9 

877 

163 

143 

136 

140 

125 

123 

127 

913 

156 

153 

145 

12C 

144 

116. 7 

257. 0 

903. 3 

165. 3 

151. 3 

143. 7 

126. 3 

137. 3 

5.5 

117.3 

23.1 

10.7 

7.6 

7.1 

11.8 

10.7 

A 

A 

\ 

l.C 

2.2 

7.7 

1.4 

1.3 

1.2 

1.1 

1.2 

MUTAGENI C 

EXP DATE: 042&63 

TEST TYPE: 05 

DILUTION: l: 20 

MIXTURE: 5C0 

I T Y 

CHEMICAL : NP 

STRAIN : TA130 

PLATED: ICC 

TECHNICIAN: LGM 

CONTRACT NO. 

ACTIVATION: 

BATC.H : D273 

COLONIES: 

: 191482 

CSDCC2 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE 

COUNT 

A : 

3t 

PLATE 

COUNT ?G 

PLATE 

CCUNT 

C: 

BC 

AVERAGE 

CCUNT 

STP DEV 

CCUNT 

O/C MR MEC 

SI 

FI 

F2 

99 

04 

C4 

c e o 

1.00 

2.0 0 

33.30 

1C u • o C 

333.CO 

KOC.C C 

3333.CO 

131 

1C56 

1619 

137 

152 

14C 

146 

166 

171 

1121 

814 

125 

150 

131 

152 

152 

142 

919 

517 

111 

132 

122 

142 

136 

1345. 3 

9^3. 3 

124. 3 

144. 7 

131. 0 

146. 7 

151. 3 

20. 7 

110. 1 

57C 2 

13. 3 

11. 3 

9 . 0 

5 . 0 

15. 0 

A 

A 

A 

1 . 0 

7 . 1 

6 . 6 

0 . a 

l . C 

0 . " 

1 . 0 

l . C 

MUTAGENICIT Y 

EXF DATE: C42t>c3 

TLST TrPE: 05 

DILUTiON : i : 20 

MIXTUPE= 5:3 

CHEMICAL : r.p 

STRAIN: T AI:3 

FLATEC = ICC 

TECHNICIAN': LO! 

CONTPAC T NO. : 19148 2 

ACTIVATION : H E PAI I 

BATCH : 0273 

COLONIES: 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE A: 

CCUNT 3G 

PLATE r> 

COUNT :• 

PLATE 

COUNT 

c: 

6fc 

AVE FAGt 

CCUN T 

S T O DE V 

CTUN T 

G /C MR HE: 

SI 

F2 

9° 

04 

04 

C C C 

0.5C 

I.JO 

33.00 

1 0 0 . 3 C 

333.00 

l r o o. o o 

3333.CO 

163 

1106 

2226 

197 

14f 

335 

161 

227 

1 BC 

1349 

2CC4 

182 

220 

176 

180 

131 

9 P 2 

22il 

191 

195 

2 05 

155 

195 

iec c 

1072.7 

2172. 3 

l

c

1 . 3 

171 . C 

2h4. 7 

164. C 

24. 9 

103. 5 

146. ° 

5 . 5 

21. 7 

73. 4 

1C.B 

23. 6 

1*0 

6 . P 

13. 7 

1.2 

1.1 

1. 6 

l . C 

1 . 3 

122 
P.32 

'I I 

r. 

R 

T 

A 

T 

2 

J 

\' 

T 

S:SCLVENT p:PCSITIVE N:ANTHRAMINE V/ 0 S9 .:TES T COMPOUND I 

C0N=1914£2 CHEM:NP STRAIN:10 0 0ATE:C42se3 ACTIV: ACTX:05 

! 

9C0.0 • 

83C 0 • 

70C 0 • 

f 

! 

60C O . 

533. 0 • 

430. 3 • 

30C C • 

2 3 C C • 

i : c c • 

• 

i 

! 

; 

CC • 

PLOT OF AVGCOUNT.COSE SYMBOL IS VALUE OF SPIND 

io i: : 

DOSE IN MICROGFAKS 

12J 

IPC S 103- 0 
P.33 

S = SOLVE NT P:POSITIV E N:ANTMRAMIN E W/C S9 *:TES T COMPOUND 

C0N = 19146 2 CHEM:f.P STRAIN:lO C CATE:C«26S 3 ACTIV=CSD00 2 ACT i = 05 

PLOT OF AVGCOUNT'OOSE SYMBOL IS VALUE OF SPINO 

1100. 0 

1C0O.0 

900. C 

600.G 

700. : 

60C. C 

see : 

4CC . 

30C. 0 

2CC.C100.c 

0 . 0 

10 

- • -

ire inc o 

- • - • -

10 0 0 0 

03SE I N MICROGRAMS 

124 
P.34 

" | • I 

•ii'Mii' m -¥wm i IIHW .(.•.• • — ' W * l '"*» • «I^I^IJ»W..I. . iuwiw^wpr«ffrwjiMjii^lLi«jWM u n>i|ii.. im «•> » M ..ity — ^ 

S:SOLVENT P=POSITIVE N:ANTHRAMINE U/0 S9 *:TEST COMPOUND 

C0N:191482 CHEM:NP STRAIN:10C DATE:C42883 ACTIV:HEPA01 ACf£=C5 

PLOT OF AVGC0UNT*D05E SYMBOL IS VALUE CF SPIND 

2250. 0 • 

; 

; 

• 

20C0. 0 • 

! 

1750. 0 

1503. 0 • 

125C C 

100 C c . 

75C 0 • 

5 C 3 . C • 

25C C • 

C O • 

io iro 

DOSE IN MICROGRAMS 

. . . . . . 

100 0 10 3 0 0 

125 
P.35 

,i ...W.^III »M„.....i...jiH .M.MHi .) . l»*W»*»»JWi!irflW>W«WWtf ^ HII^JHUMfl^-*J»CT 

MUTAGE ,ICITY ;0F N8 - JA9 7 

1 U T A G 

EXP DATE : 

TEST TYPE: 

DILUTION: 

MIXTURE: 

SOLVENT/ 

POSITIVE 

E N I C I T Y 

C42883 CHEMICAL : *<n 

05 STRAIN.* TA97 

: PLATED: 

. TECHNICIAN: L'->* 

DOSE 

LEVEL 

SI 

PI 

P2 

99 

02 

02 

0.3C 

5C0 C 

100.03 

33.00 

1 0 C • 3 3 

333.00 

1POCC 0 

3333.00 

PLATE A : 

COUNT 3G 

132 

562 

1222 

12n 

143 

12 0 

115 

PLATE e: 

COUNT -.G 

124 

473 

951 

145 

i4* 

14S 

139 

76 

CONTRACT NO.: 191482 

ACTIVATION: 

BATCH.* o?73 

[COLONIES: 

PL**TE c: 

COUNT BG 

AVERAGE 

COUNT 

115 123. 7 

346 460. 3 

U 9 3 1155. 3 

131 134. 7 

13 0 13 9. If 

13C 134. 3 

145 14 0.7 

77 9C 0 

STD DEV 

CCUNT 

8 . 5 

108. 6 

277. 1 

9 . 1 

7 . 8 

14. 0 

3 . 8 

21. 7 

O/C MR fit 

A 

A 

A 

Uo 

3 . 7 

9 . 3 

1.1 

1 . 1 

1 .) 

1.1 

C 7 

MUTAGENICIT Y 

EXP DATE: 342683 

TEST TYPE.* 05 

DILUTION: l : 20 

MIXTURE: 500 

CHEMICAL : \fa 

STRAIN : TAS7 

PLATED: ICC 

TECHNICIAN: L >M 

CONTRACT NO.: 191462 

ACTIVATICN : csooo2 

BATCH : 0273 

COLONIES: 

SOLVENT / 

POSITIV E 

DOSE 

LEVE L 

PLAT E A *. 

COUN T 5G 

PLATE •< : 

CCUNT r-i. 

PLATE C: 

COUNT BG 

AVERAGE 

COUNT 

STD DEV 

COUNT 

8/C MR MEC 

31 

PI 

P2 

99 

04 

04 

O.CC 

1.0C 

2.30 

33. 32 

133.J 3 

333.00 

1C3 C cc 

3333.CO 

134 

5 = 9 

1 "fcc 

154 

145 

1 6a 

171 

15C 

lit 

6 = 9 

12:7 

1 -«. 

l'-5 

174 

17t 

1 7: 

12 = 

716 

1149 

133 

189 

175 

163 

165 

12r.C 

6 71. 3 

115 S.C 

1 4 c. 3 

173.C 

174.C 

16°. 7 

161. 7 

5 . 3 

63. 2 

94. 6 

11. 6 

24. 3 

5 . 0 

6 . 1 

1 C4 

1.3 

5 . 2 

9 . 0 

1.1 

1.3 

1.3 

1.3 

MUTAGENICIT Y 

ZxP DATE: 04?t» 3 CHEMICAL : -i-i 

TES T TYP: : 35 STRAIN : TA= 7 

3ILU7ION : i : 20 hLATEO: 1 -3 2 

MIXTURE: 513 TECHNICIAN: ; 

C C T^ACT NO.: 

ACTIVATICN : Hi 

BATCt-: 02 7 3 

COLON!:s = 

19 1 *• e-2 

PA 1 

SOLVENT / 

POSITIV E 

DOS E 

LEVE L 

PLAT E A 

COUN T til 

PLAT E 

T3UN T 

PLAT : 

CCU\ T bO 

A V L F i C L 

CU\ T 

STD OEV 

CCUNT 

U/C *"? MEC 

SI 

M 

°2 

qc 

&4 

•3 4 

.> . J --

0.50 

1.00 

11. CO 

i c v. •:: 

233.0 0 

i: c c :: 

3 3 3 5 . C C 

14 2 

4 = ' 

1 0 2 2 

let 

142 

171 

14* 

1 : ^ 

2 • 6 

lf-7 

151 

13 = 6 

115 

lfil 

13r. 7 

ib'n C 

1 0 3 3. D 

It 7.2 

163.3 

It. 7.7 

134.7 

16. 3 

73. 0 

4$. r 

is.: 

i°. p 

U.r, 

5 . 7 

1.' 

7.c 

i.: 

1.2 

1... 

1.5 

126 
upjWUWPUSasppe lif^llf.jjii^.^^^pg^lW^^pij i 

CCN 

1530. C 

1400. 0 

1303. 0 

1230. C 

1KC. C 

R 1033. 0 

3 

U 

N 

T 

S:SOLVENT P=FCSITIVE N:ANTHRAVINE k/$ S9 *:TEST COMPOUND 

:19148 2 CHEH:N3 STRAIN: 97 DATE::42«ie3 ACTIV: ACT*:©? " 

PLOT OF AVGCOUNT'DOSE SYMBOL IS VALUE CF SPIND 

! 

• 

! 

f 

• 

! 

! 

• 

! 

! 

• 

! 

. 

! 

• 

! 

! 

• 

! 

f 

• 

930. 3 

3 3 0. 3 

7C3. 0 

bCO.O 

\t\i» 3 • 

4C3.C • 

333. C • 

2 3G« 

! S 

100. c • 

! 

; 

0.0 • 

— - 4 • 

io ir e 

DOSE IN MICROGRAMS 

10: 0 i c o : o 

127 
P.37 

laurn'- -wjl^-Jiijy.'S' T y l'WP^^^^?ft^i^»iw^^.ViW 

S:SOLVENT P:P0SITIVE N:ANTHRAMINE w/0 S9 «:TEST COMPOUND 

C0N:191482 CHEM:N3 STRAIN:97 DATE:C42663 ACTIV:CSD002 A3TX:C5 

PLOT OF AVGC3UNT*D0SE SYMBOL IS VALUE OF SPIND 

V 

r 

R 

T 

A 

N 

T 

z 

0 

•J 

N 

T 

! 

1500. 0 • 

• 

! 

1*00.0 • 

! 

! 

1300. 0 • 

! 

• 

123CC • 

! 

! 

11C0.0 • 

! 

! 

1300.C • 

! 

! 

eOC.C * 

I 

! 

i C 3 . C « 

! 

i 

7C0. 0 • 

• 

i 

t CC C * 

5 0 C . 0 

4CCC • 

! 

i 

30C C • 

i 

• 

2CC.0 • 

! 

• S 

1CC 0 • 

I 

I 

c c • 

IC 100 

DOSE IN MCRCCP.AMS 

1CC 0 100 JO 

126 
P.38 

S=SOLVENT P:POSITIVE N:ANTHRAMINE W/0 S9 *:TEST COMPOUND 

C0N=191462 CHEM:NB STRAIN:97 0ATE:C42683 ACTiV:HCPA01 ACT*=05 

1100. 0 

1000. 0 

900. 0 

300. 0 

700. 0 

60C.0 

&C0.0 

400. 0 

3 C 0 . 0 

200.C 

100. 0 

0.0 

PLOT OF AVGCCUNT'DOSC SYMBOL IS VALUE OF SPIND 

SOTE: 

3 1 10 IC C 

PCS E IN *ICR - .-RAMS 

1 O^ S HA D MISSIN G VALUE S OR WER E CU T Of RANG E 

1 C 0 C C 
MUTAGENICIT Y OF NB - TA9S 

P.39 

MUTAGENICIT Y 

E XP DATE : 04288 3 CHEMICAL : NB 

TEST TYPE : 05 STRAIN : TA96 

DILUTION : : PLATED : 

MIXTURE : TECHNICIAN : LGM 

CONTRAC T NO. : 1914E2 

ACTIVATION : 

BATCH : 027 3 

COLONIES : 

SOLVENT / 

POSITIV E 

DOSE 

LEVE L 

PLATE 

COUN T 

A : 

BG 

PLAT E B : 

COUN T BG 

PLATE C : 

COUNT BG 

AVEPAGE 

COUNT 

STD DEV 

COUNT 

G/C MR MEC 

SI 

PI 

P2 

99 

03 

03 

3.0C 

1.00 

2.CO 

33.00 

100.CO 

333.00 

lCOO.Cu 

3333.00 

13 

214 

346 

26 

31 

32 

32 

20 

14 

192 

343 

31 

39 

27 

32 

7 

4 

171 

321 

20 

31 

29 

32 

29 

1C.3 

192. 3 

336. 7 

25. 7 

33. 7 

29. 3 

32. 0 

13. 7 

5 . 5 

21. 5 

13. 7 

5 . 5 

4 . 6 

2 . 5 

0 . 0 

11. 1 

NA 

A 

A 

l . C 

I F . 7 

32. 7 

2 . 5 

3 . 3 

2.e 

3 . 1 

il. P 

MUTAGE N I C 

•iXP DATE: C4268 3 

TEST TYPE : C5 

DILUTION : 1.* 2 0 

MIXTURE : SCO 

I T Y 

C-EMICAL : NB 

STRAIN: TA98 

PLATED: 130 

TECHNICIAN: LGM 

CONTRACT NC.: 1914P2 

ACTIVATION: CSDCC2 

BATCH: D273 

COLONIES: 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE 

COUNT 

A : 

EG 

PLATE 6: 

COUNT [)G 

PLATE 

COUNT 

Cf 

BG 

AVERAGE 

COUNT 

STO DEV 

COUNT 

Q/C Mfi MEC 

SI 

PI 

P2 

99 

04 

C4 

o.oc 

1.00 

2.0.0 

33.CO 

100.00 

333.CC 

1CCC.CG 

3333.CC 

3C 

622 

16P6 

42 

45 

47 

49 

36 

42 

673 

1661 

41 

bC 

36 

33 

44 

42 

6fc2 

17«4 

38 

36 

46 

49 

45 

36.0 

658.0 

1713.7 

4 C 3 

43.7 

43.C 

42.7 

42.3 

6.9 

31.7 

70.7 

2.1 

7.1 

6.1 

11. C 

3.A 

l.C 

17.3 

45.1 

1.1 

1.2 

1.1 

•1.1 

1.1 

MUTAGENICIT Y 

EXP DATE: C42&83 

TEST TYPE : 05 

DILUTION: i; 20 

MIXTURE: 530 

CHEMICAL : NB 

STRAIN : TA5 3 

PLATED: ICC 

TECHNICIAN: LGM 

CONTRACT NO, 

AC TI VATIC*. : 

PATCH: D373 

COLONIES: 

: 1914E2 

HEFA71 

SOLVENT/ 

POSITIVE 

COSE 

LEVEL 

PLATE A: 

CCUNT bG 

PLATE fa: 

CCUNT b& 

PLATE 

COUNT 

c: 

66 

AVERAGE 

COUNT 

STC DEV 

COUNT 

C/C MR MEC 

SI 

PI 

P2 

04 

04 

0.3 0 

0.5 0 

1.3 3 

33.CO 

100.0 0 

333.3 0 

1CCC 3C 

3333.0 0 

43 

407 

351 

46 

42 

47 

31 

2R 

40 

574 

1213 

32 

54 

32 

42 

35 

31 

6C7 

1747 

27 

42 

3 = 

25 

42 

3F. 0 

52C 3 

1 4 3 7. C 

35.G 

46.C 

3

C

. 3 

32. 7 

35. 0 

1C7. 2 

2 77. 2 

9 . F 

6 . 9 

7 . 5 

8 . 6 

7 . 3 

i . c 

13. a 

3 7 . f 

0 . ° 

J 1. 2 

l . C 

c . c 

130 
P.40 

t>; 

v 

r 

R 

T 

A 

N 

T 

D 

U 

N 

t 

S:SOLVEN T P:P0SITIV E N:JNTMRA"I\ E W/0 S° « = TES T C0MP01i«n 

CGN = 191462 CHEM = N6 STRAIN=98- C ATE = 0 42££ 3 AC Tl5= A'CTX = ! 

PLOT OF AVGCCUNT.DUS E SYMBOL IS VALUE OF SPIN D 

330.0 • 

i 

300. 0 • 

270. 0 • 

240. 0 « 

210. G • 

16C 3 • 

15CC • 

i 

f 

12C C • 

9 C 0 • 

i 

! 

; 

6 C 0 . 

• 

; 

! 

3 C C •. 

i 

! 

! S 

C O • 

10 i : c 1CC 3 ic o: c 

COSE I N VICRCGPAMS 

131 
P.41 

S:SOLVENT P:FDSITIVE N:ANTHPAMINE k/ 0 S9 *:TEST COMPOUND 

CCN:191482 CHEM:N3 STRAIN=96 pATE:C42cS3 ACTIV:CSOOC2 ACTX:C5 

i 

i 

r 

i 

1 

I 

R 

i r 

V 

r 

R 

T 

A 

N 

T 

** *. 

rs 

u 

N 

T 

1600. 0 

1600. 3 

1400. 0 

i2cc : 

10C0.G 

6CCC 

fcCO.O 

4CC.3 

Z3C C 

O.C 

• 

I 

! 

! 

I 

• 

! 

I 

I 

• 

• 

f 

! 

; 

• 

« 

! 

• 

! 

! 

• 

i 

t 

! 

i 

• 

; 

; 

; 

i 

• 

! 

! 

• 

! 

• 

! 

i 

• 

• 

I 

! 

; 

• 

PLOT GF AVGCOUNT.OOSE SYMBOL IS VALUE OF SPIND 

10. ICG 

DOSE IN MICRO-RAMS 

1000 1 0 C '. 0 

132 
P.42 

S:SOLVENT P=f&$ITIV E N:ANTHRAMINE k/ 3 S9 «:TES T COMPOUND ; 

C0U=19148 2 CH.CM:N3 STRAIN:9 6 DATE:C42ee 3 ACTIV:h£PA0 1 ACTX:3 5 

T 

A 

N 

T 

! 

1500.C • 

1400.0 • 

1300.0 • 

1200.0 • 

1100.0 • 

1000.0 • 

900.0 • 

800.0 • 

700. : • 

6C0 . C 

SCO. " 

400. 0 

3 0 0. 3 

200. 3 

10C C • 

C C • 

PLOT CF AVGCOUNT.DCSE SYMBOL I S VALUE OF SPIN D 

IC ICC 

DOSE I N MICROGRAMS 

103 0 

133 
MUTAGENICIT Y CF NB - TA10C 

P.43 

MUTAGENICIT Y 

E X P DATE : 04256 3 CHEMICAL : N B 

TES T TYPE : 05 STRAIN : TAIO O 

DILUTION : : PLATED : 

MIXTURE : TECHNICIAN : LGM 

CONTRACT NO.; 

ACTIVATION: 

BATCh: D273 

COLONIES: 

191482 

SOLVENT/ 

POSITIVE 

DOSE 

LEVE: 

PLATE A: 

.COUNT BG 

PLATE B : 

COUNT bG 

PLATE c : 

COUNT BC 

AVERAGE 

COUNT 

STD OEV 

CCUNT 

O/C MR MEC 

SI 

PI 

P2 

99 

01 

01 

0.00 

1.00 

2.0G 

33.CC 

100.33 

333.30 

lCCCOC 

3333.CO 

114 

355 

920 

134 

96 

111 

91 

113 

113 

289 

877 

133 

127 

153 

95 

108 

123 

127 

913 

135 

152 

1C9 

98 

99 

116. 7 

257. 0 

9C3.3 

134. 0 

125. 0 

124. 3 

94. 7 

106. 7 

5 . 5 

117. 3 

23. 1 

1.0 

2 8 . 1 

24. 3 

3 . 5 

7 . 1 

1.0 

2.2 

7.7 

1.1 

1.1 

1.1 

C d 

0.9 

MUTAGENICIT Y 

E XP CATE : C42C6 3 

TES T TYPE : 05 

DILUTION: i: 20 

MIXTURE : b'cO 

CHEMICAL : NB 

STRAIn: TAlOO 

PLATED: ICC 

TECHNICIAN: LGM 

CONTRACT NC : 191462 

AC TIVATICN: CSO0C2 

BATCH: D273 

COLONIES: 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE 

CCUNT 

A : 

30 

PLATE

 r' 

CCUNT 

\3 • PLATE C: 

COUNT 60 

AVERAGE 

COUNT 

STD OEV 

COUNT 

Q/C MR MEC 

SI 

PI 

P2 

04 

C4 

O.OC 

l.CC 

2.CC 

33.30 

10 3.00 

333.30 

1 C 0 C 0 c 

3333.00 

131 

10*6 

1619 

116 

112 

137 

155 

124 

171 

1121 

614 

130 

115 

126 

13? 

127 

142 

919 

517 

135 

127 

193 

142 

137 

14h.G 

1C45. 3 

9*3. 3 

127. 3 

ue. c 

152. C 

145. C 

129. 3 

2 3 . 7 

110. 1 

570. 2 

9 . 8 

7 . 9 

35. 9 

8 . 9 

6 . 6 

l . C 

7 . 1 

6 . 6 

0.° 

0 . 5 

l . G 

l . C 

0 . 9 

M U T A ICIT Y 

ExP DATE: :42t'.t3 

TEST TYPE: 35 

DILUTION : l: 20 

MIATURE : 5IC 

CHEMICAL : NB 

STRAIN : TA1CC 

FLATEC : 13C 

TECHNICIAN : L3H 

CCNTFAC T NO. : 19148 2 

ACTIVATION : HEPA; I 

BATCH : D?7 3 

COLONIES : 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE A : 

COUNT bG 

PLATE s: 

COUNT EO 

PLATE 

COUNT 

c: 

BO 

A V L P A G E 

CCU..T 

S T D DE V 

COUNT 

O/C MR MEC 

SI 99 

PI O H 

P2 3 4 

r •

, * 

0.5 3 

i.: : 

33.30 

« "i » * ^ 

1 J M • h f 

333.CO 

1 ? 0 C 0 3 

3333.OC 

163 

1166 

2226 

12fi 

147 

13C 

124 

7c 

ISC 

IC*? 

2;: 4 

134 

129 

136 

112 

11° 

131 

9c3 

22 8 7 

119 

126 

123 

123 

87 

15 c. 0 

1C72. 7 

217 2. 3 

127. 0 

134.r 

12r. 7 

119. 7 

94. 7 

24. 9 

1C3. 5 

148. 9 

7 . 5 

11. 4 

6 . 5 

6 . 7 

21. 5 

l . C 

k.* 

13. 7 

O.S 

0 . 5 

r. .•; 

0 . 9 

0 r 6 

134 
P.44 

T 

A 

N 

T 

0 

J 

N 

T 

S:SOLVENT P:P3SITIVE N = ANT'HR AM I NE W/0 S9 *:TEST COMPOU'JO 

C0N:1914c2 ChEM:NB STRAIN:1C0 CATE:Q42P83 ACTIV: AC U = 05 

! 

900. 0 • 

i 

! 

i 

• 

600. 0 • 

700, 

600 . 

500. 0 • 

43C ? • 

30C.0 • 

2 3 C C • 

100. 0 

C C * 

PLOT OF AVGCOUNT^DOSE SYMBOL IS VALUE OF SPIND 

ic i: c 

DOSE IN MICROGRAMS 

13 0 3 i : o ; o 

135 
P.45 

S:SOLVENT P:P0SITIVE N:ANTHR A*'I NE to/0 S9 *:TEST COMPOUND 

C0N:191432 CHEM:N3 STRAIN:100 DATE:C42fc83 ACTIV:CSD0C2 ACTi:0 5 

PLOT OF AVGCOUNT.OOSE SYMBOL IS VALUE CF SPIND 

! 

; 

1100. 0 • 

I 

t 

J 

1000. 0 • 

I 

I 

! 

900. 0 • 

i 

i 

• 

80C O • 

• 

70C C • 

i 

• 

i 

60C G • 

• 

! 

i 

530. 0 • 

• 

i 

; 

40C C • 

I 

I 

I 

300. 3 • 

i 

• 

i 

230.C • 

« 

; 

i 

100. 0 • 

I 

I 

I 

c c • 

10 ICC 

T3SE IN MICROGRAMS 

10C 3 i r c: o 

136 
P.46 

— ••-- — 

S:SOLVENT P=POSITIVE N:ANTHRAMINE W/0 S° *:TEST COMPOUND 

CCN:191462 CHEM:N3 STRAIN=1CC DA TE: G<426 ^ 3 ACTIV:HEPA0 1 ACTX:05 

22bO.C • 

i 

! 

! 

! 

2000.0 • 

! 

! 

! 

» 

17bG.O • 

! 

! 

j 

• 

15CG.0 • 

! 

! 

! 

125C.0 

C O 

7tO.C 

5CC.C 

25C.0 

C C 

PLOT OF AVGCOoNT*COSE SYMBOL IS VALUE CF SPIND 

10 iro 

DOSE IN MICSwCRAMS 

inoo 

137 
P.47 

* • * TM ' * " i n u«ww 't ^T*1

"^. ntr-m**1.—! iif«-» .wi'7 npipjyn P. I.I W 

MUTAG;..wCITY CF HS - TA?7 

M U T fc G 

EXF DATE : 

TEST TYPE ; 

DILUTION : 

MIXTUFE : 

E N I C I T Y 

! 4 2 C i CHEMICAL : M 0 

STf A 11. .* TA"7 

PLATE!": 

TECHNICIAN: D"$ 

CC'TfACT NC". : 191 4f 2 

ACTIVATION : 

PATCH : Dl r3 

COLO' IES: 

SOLVENT/ 

'OSITIVE 

DOSE 

LEVEL 

PLAT E A : 

COUNT bG 

FLATE S*. 

COUNT i.O 

FLATE C: 

COUNT CC 

AVERAGE 

COUNT 

STD OEV 

COUNT 

O/C MR MEC 

SI 

PI 

F2 

55 

02 

32 

O.C-3 

50.3 0 

103.33 

17..: 

5 7. :c175.:: 

5-2.:-: 

175C3 C 

140 

42*v 

lC-t 

15: 

l«-3 

1 4 2 

lfel 

1 2 9 

433 

11C 6 

153 

1S2 

136 

141 

«- o 

H I 

414 

"9 7 

152 

137 

lfrl 

c 4 

1 1 6 

1«• C . C 

425.3 

1C67.C 

151.7 

157.3 

1 41. 3 

i *; £. • t 

c

r . 7 

27. 4 

13. 3 

feC? 

1 . 5 

23. 5 

13. 1 

3 4 . 4 

29. 7 

2.= 

7 . 1 

1 . 3 

1 . 3 

l . C 

C 9 

C 6 

MUTAGENI C 

E>F DATE: :423; 3 

TEST TYPE: :•: 

DILUTION : i : 2 3 

<*iXTU?E : 

4k • 

I T Y 

CHEMICAL: HS 

ST=.tli : Ti- 7 

PLATL ! : 1" : 

TECHNICIAN : C" S 

CC!.T°tC T VO. : 19l4c 2 

ACTIVATION : CSO:C ? 

hiTCI.: Dl- 2 

CLCMTS : 

SOLV-ILT/ 

»OSIT;V : 

oo?-: 

LLVEL CC-UNT L'O 

t- L t T •: 

Co'. T 

( • • f- LATE c: 

C'.Uf.T HC 

AVERAGE 

COUNT 

STP LEV 

CuUNT 

C>; KR 

31 55 

r'l C4 

R2 : 4 

1 . 

& . • • 

1 7 . 

5 7 . 

175 . 

Lh2 . 

17V. . 

177 

I4f.t 

! ' i 

154 

I 6 r 

2 C 

l c 3 

f - 5 

,1c ? 

1 7 4 

1 7 7 

1 - 1 

2 . 3 

l r l 

7C3 

2 1 : 

IT; 

! 7 : 

17 2. 7 

Ltr.l 

1 2 1 - . C 

1 7C C 

1( C C 

1 r '-. 3 

i

c

c c 

1-1. 3 

11. 2 

t 3 . 3 

!23 . 1 

24. f 

1 1 .

 c

. 

26. 5 

14. 2 

i . : 

7 . 1 

i . : 

l . : 

l . i 

i . : 

1 . 1 

«• v T i : 

Ex; DiTti: 

TEST TYP: 

JILUTK-N: 

M I X T U -1 : 

E •' I C 

. 4 ;•; c 3 

s • 

ITY . 

Cr-E^ICiL: h< 

ST-.ii;. : T-- 7 

TLATL"? : i'-: 

TECr:NIC!A..= • 

CCTr iCT 

iCTiv:TI C ur' A :: 

tI'l * "". -* 

CM-:M:JSOLVENT/ 

'vSITI vE 

31 55 

= 1 34 

*L 3 4 

L-3SE 

LEVE L 

1 

17 

5 7 

17b 

'-•f.2 

175: 

FLAT" t: 

CCUNT iO 

19* 

7-3 

' ' *i 

i U ^* I 

l

u ,-

if t. 

It, 7 

i : 4 

: 7 ; 

F L A T -: 

C3o'.T 

174 

-S7 

:"" 9 

. 7 ~ 

U : 

lit 

1 75 

F LAT C 

C wJ'.-T 

1 ' 3 

i:t. : 

13r-

11; t 

114 

i t : 

2 : 4 

V . . 

b( 

: v:'. t :, i 

C-U..T 

i

;

- L . :• 

;•<•.- • -

13 31. 7 

143. 3 

11 '. . 7 

143. 7 

1/7. 3 

i -• j. : 

: T L> c •: v 

CU'-.T 

1 C 7 

4 C 3 

* 2 C ** 

14. : 

5 . 3 

2 7 . 1 

3 1 . -

17. -

/ r v 

4. •. 

138 Reproduced Irom 

bosl available co ro, 
P.48 

pun.ni .H,.IUMJJ.. i . w . «.i.n.w ni ' mmi.i^L m. ''••m'.vmmjjfjftu. timyiiMvn m i \,tmmt.~* i«Jiqu»inpii.JUKi»i'n.j|jgjcpM.«.ijm,—jimm.miw«n.|]w«L..iij 

S:SOLVEN T P:F0SITIV E N=AN T MP6 " I'.'E w/C S* »:TES T COVP-JL'NC 

CCN:1914f,2 ChEM:h S STF.tIN:=7 DA TE =042'..P3 ACTIV : ACTt = 35 

PLC-T OF AVGCCU.T*D3S E SYMBO L IS VALU E CF SPIN D 

11C0. C • 

1C-3C.0 • 

. M . c • 

t 0 C 0 • 

7 3 3.3 • 

&o:. c * 

. J . .• • 

1 3 C C • 

0. C • 

1 " 1 '. : 

POS E IN- "ICf^C r.civc 

1 : ; a 

139 
P.49 

fli W" W - " ' "» W •JUMWWJjm l | U+VTim^mmixmmj^mmmmmGmmmjmi "V^^fv^i mM£twm, M y WWffm?gww g 

S=S3LVENT P=F0$ITIV E N=ANTHR/.MiNf w/ 0 S= -:T:S T COMPOUND 

CCIJ:l = 14c 2 CHLM:HS STRAl'j:? 7 CATE:C42C? 3 ACT1V:CSDCC2 A 

PLOT :F AVGCOUNT.OOSE SYKCG L IS VALUE CF SPIN S 

Ct*=3 5 

s 

V 

r 

R 

T 

A 

T 

w 

3 

1 

\ 

T 

150C C 

14C0. C 

1330. 0 

12 C 3 . 3 

11CC. C 

i : : c c 

- c o 

rCC C 

7 <J w • u 

c *> » • i 

5 c: . c 

A c; . [ 

t r r 

V <m » • w 

<L ., - • . 

1 0 c c 

I 

I 

• 

i 

• 

i 

i 

• 

! 

i 

• 

i 

• 

• 

i 

• 

i 

i 

• 

i 

• 

! 

i 

• 

i 

t 

4 

J 

1 

• 

j 

; 

• 

i 

1 

• 

• 

1 

1 3 

.I.JSE IN !-• I C P . 

i : : 

. . . 4 . 

i r :•: 

140 
wgBBWj^'^J^^^*^^ 

S:SOLVENT P:POSITIVE N:ANTHRA"M NE k/0 SS »:T(iT COi-'.PC UNr: 

C0N:i?14o2 CHEM:M S STP.A1N:57 DATE:C4::P. 3 ACTlV=hEPACl ACTJf = C 

I 

PLOT OF AVGCOUNW'C-SE SYMBOL IS VALUE OF SFT.0 

1100. 0 

1000. 0 

=oc: 

! 

t 

• 

! 

! 

; 

• 

! 

! 

i 

• 

P-CCf • 

730. 3 • 

c. O 3 . C • 

5CCC • 

43c : • 

3 C 3. C • 

c w. u • u 

K' C i' • 

CC • 

i : i •: : 

[•"OE I-" J-" I C 3 r-0

 s A!'E 

1 0 3 

141 
MUTAGENICITY OF hS - TA9-

P.51 

» U T A G E N 1 C I T Y 

: X P DATE : :<*2:'b2) CHEMICAL - : H S 

TES T TYFT : 05 

DILUTION : : 

MIXTURE: 

STRAIT,: TAc e 

PLATED: 

TECHNICIAN: Uv ! 

CCf.TF.ACT NO.: 

ACTIVATIC:; : 

bATcn: cir-3 

COLONIES: 

1914a: 

SOLVENT/ DOSE PLATE A: FLATE u 

POSITIVE LEVEL COUNT tn, COUNT r 

D LAT E c: AVEC A:.E 

CCUNT PC COUNT 

STD DEV G/C 

COUNT 

M.R 

SI 55 

FI 04 

?2 C-4 

C • - V 

1.0 0 

2.rr 

17.c: 

57.3 3 

175.3 3 

5c:2. 3? 

1753. it: 

21 

1?7 

334 

2 7 

12 

13 

16 

20 

2:2 

322 

. c 

23 

14 

16 

5 

"UTAGF.I * ICIT Y 

EXr DATE : 'H2^.-3 CHEMICAL : nS 

TES T TYPI : :r; STFAIN : T- = S 

DILUTIC-J: i: ro PL-TE:>: I C 

«IxTU-:: b • C- TCC*'. ICI A'. = -''' 

SOLVENT / c:'r.E

 rL^T-: :: PLAI T •• 

s-CSITIV i LEVEL CCU..T b ( CM'N T 

S I c : : 

l . •. : 

2 . : c 

1 7 . - : 

57.: : 

: 7-:. a 

L «•• 2.. : 

1 7 t. C . . t 

6 ~ 5 

137 -

C ** 

35 

13 

1*-

2 7 

7 1 9 

1 t __ s 

32 

2 7 

2 2 

2 ? 

v

 0 T ' 3 

EXr DATE: 

r

 ': I C 

-

. 4 i :•<.? 

TEST T>Pf : (.-j 

jILUTIO'- : J : 2C 

*•. I/TU-E : tO u 

T v 

Cr.EMlCAL : r.S 

ST-.il'. : TA- ^ 

PLATE: : I : ' 

TECnNiCIAN : i 

C .' 

10 7 

342 

14 

15 

21 

irCC ^TF 

.' C T1 V 

D A T C1 

C' 

P L' TE 

C-:U-.T 

^ C743 

151 7 

1

 J 

3

r 

2 1 

3 -

?2 

CC 

LCN 

C: 

P.G 

ACTI V 

r. ,• 

c: 

TCtLCr 

2 C 3 

le5.3 

333.0 

1=.3 

16.7 

lb.3 

17.? 

11.3 

« 

/•C T '.•...: 

A TIC. : C S 

: DI- 3 

irs = 

A v:r . A G ." 

CUNT 

2L.C7i c : 

i 4 f i'.: 

21.. 0 

2r.3 

2 4.3 

21.7 

~ CT i. • . 

-TIC:.: r.:. 

: T: 1 - 3 

IE?: 

C 6 

73.2 

1C5. 

e.4 

5.7 

4.4 

1.7 

7.?. 

1

 c J -4 f 2 

ore: 

J T j CE V 

c :u*. T 

4.4 

24 . G 

77.2 

7.2 

7.t 

3.1 

f.5 

1.5 

19l4r2 

(- -:; 

A 

1 . 3 

C l 

.6. 4 

l . C 

C

f c 

U • * 

C F 

(. / C K 

i . : 

32. 7 

St . 7 

1 . 2 

1 . 3 

1 . 1 

K-EC 

S:LV.-.-T / 

= c s i T : v : 

i ' - * '. 

L •: v •: L 

c

 L A T r A : 

:CUN T bC 

PLATE 

CUN T 

L

LAT L C : • A V E - • N E ST: r: v c / c 

COUNT c:- C'-ir.T r :U-

.T 

v

rc 

r 1 3 4 

•-2 C4 

C 5 '!' 

I.. . 

17.: : 

5 7 . j : 

175 .-: c 

b

 : :2 . c .. 

7",:., - <•• 

44 

le.2 . 

17 

1.1;-. 

27 

•• i 

: 7 

j ; 

^i 

5 : 7 

ic=-

2 : 

34 

2 " 

?C 

._ 1 . u 

5 3 4.. . 

1?=-:. 3 

2 ;••. J 

- A i 

«_ *1 • *. 

4 ^ • -

It. 7 

7 . r 

i ::. : 

y -. 4. 7 

7.-:-

2 . c 

5 . 5 

6 . 3 

4 -. C 

i . : 

2 2. 3 

r7.r . 

1 .: • 

. . ^ 

1 . . 

c . -

I . 7 

g Scproduccd from 

best available copy, 
P.52 

S = SOLVL'iT P = hOSITIV£ \sAKTHUKU. T W/0 $c -=T:b T COKfOtUD 

C0\' = l?l«e 2 CHLM = hS STKUNa*?6 CATt. = :<• 2 ~»3 ACTIV= tr.TXr- 5 

PLOT CF ftVGCOOM*DOSE SYMcCL IS VALUE Cr SF I'.T 

1 

330 . C 

3G0.C 

273.C 

21:. : 

lac : 

i t : 

i 2: . c 

eD»: 

3C. : 

c. 

i" K : 

Sf It. M r POOF*": 

I : *..*. . 

143 
P.53 

U 

M 

T 

S:S3LVENT P:P;SITIV £ '. :ANTHF.A" IN : W/C S? «=Tr3T C ? I1'P 0 IM [• 

CC;N = l = 14f 2 CHE"TH S STPU'.':? * C'A T E:C 4 20 «• 3 ACT1«:CSDCC2 A;T;=r 5 

PLOT OF AVOCCU'.T.DOOE SYMdCL IS VALUE CF i*>I\r, 

15DC C • 

14 0 C C • 

13CC C • 

113C C • 

10CC C • 

~:c r • 

i 

:

r C . C • 

7 3 c : • 

fc C 3 . 3 • 

43C.0 • 

3 3 c : 

1 3 ; 

0.3 • 

l f i - : 

sr IN *• iCF.:.c?A"i 

ir: : l:: : c 

14. 
P.54 

S=SOLVE*.T F-TPOSIT I VE '. :ANT HRA H I».r */ : y 

C0N=l-14c 2 ChLMr^S ST«AI\==f c D'TE::42:; ; 

= Tr ST CC'-'PDU .:. 

.CTiV:rtC.t: i A C T «. = T 5 

PLOT -.F tVuCOUNT.COSE SYKPOL I S VALUE OF SP I 

c 

r 

V 

r 

9 

T 

A 

15C0.C 

14CC3 

13CC0 

123.. : 

1133.C 

i::o. : 

f

 3 c 0 

•.33.: 

• 

• 

• 

t 

• 

• 

• 

• 

• 

• 

• 

i 

* 

; 

t 

• 

7 : C 3 • 

, 3 C C • 

4 C 

ice : • 

f s 

i ~: n: l * c : c 

• :S L i •. v • CC.'.-s Al--

145 
P.55 

MUTAGENICIT Y OF hS T A i :• •: 

M U T A G 

EXF DATE: 

TEST TYPE 

DILUTION: 

MIXTURE: 

SOLVENT/ 

POSITIVE 

E N I C 

C4:>.3 

: 35 

T Y 

CHEMICAL: HS 

STHAIN: TAI:-; 

PLATEH : 

TECHNICIAN : D" 3 

CCNTPACT >'J. 

ACTIVATION: 

BATCH : CI C 3 

COLONIES : 

1914&2 

DOSE 

LEVEL 

S I 

PI 

5 5 

31 

31 

0 3 

0. 

1. 

2 • J * 

17.3 3 

57.3 3 

175 . ::• 

5^2.: " 

175C0 C 

=>LATE A : 

CCU::T RG 

l b 7 

« 7 5 

lfcl i 

1 6 -

1 5 5 

1 2 3 

1 51 

1 ? 7 

PLATE 

C°UNT 

1 5 3 

114 3 

213° . 

1 7 5 

1 4 6 

i t a 

1 4 ? 

1 T 3 

PLATE C: 

COUNT BG 

1 6 c 

I7c t 

17« 0 

1"1 

1 ° 2 

7 7 5 

l

c

5 

1 4 5 

-UTAGENI C 

E X P DATE : :<.2CS3 

TEST TYPE : (5 

DILUTION: I: 2 0 

MIXTURE: s:-0 

T Y 

ChfMlCAL: HS 

^TF.Al'.: TA1 C 

rLAT«T: I C 

TECH* ICIAN-: CVS 

SOLVENT/ 

PC-SITIVE 

Sl 

FI 

5D 

04 

DCS" 

LEVEL 

c r: 

l . •:• •: 

2 » -

17C: 

5 7.:: 

ni.y. 

5 (•" 2 . :• c' 

1175CCC-

-LATE 

cru'.'T 

17 3 

T= -

y l l 

14-:-

1 7t 

lc E 

1 - -

If 4 

t: PLAT E 

C'-UNT 

17f 

5r° 

f 5 1 

If-6 

1 51 

1:6 

1 7? 

13<-

HO 

«! U T A G E 'i 1 C 

rXf DATE : 142C 3 

TEST TYPT: J-: 

DILUTION: :: 2: 

-IXTUFE : 0* 3 

I T i 

C^L'-ICAL : HS 

ST-:il'. : TAi C 

PLAT:O = I:.-

TETHNICIAN : •::• 

SOLVENT / 

POSITIVE 

S l 55 

P I C4 

PL 04 

LCS i 

L E V " L 

o . :•: 

C 5 C 

i.: : 

17.: : 

5 7 . :-f. 

175.: : 

5 k :• . •: : 

1753.: 3 

* LAT ' 

C U'.T 

U » 

1 1 2 2 

- . . . t. 

1 5 5 

l r 7 

1 5 5 

1^4 

FLU E iCJu'. T c i 

1"5 

123 2 

212 1 

1 3 5 

141? 

£•: 4 

1 5 0 

AVERAG E 

COUNT 

163. 3 

12*5. 3 

1F.31. 7 

17 5. 0 

164. 3 

335. 3 

162. 3 

175. C 

STD Ci V 

COUNT 

? . 7 

417. 9 

273. 7 

6 . 3 

24. 4 

3e0. S 

2 C 1 

2 C 2 

0 / C KR 

1 . 3 

7 . " 

11. 2 

1 . 1 

1 . 0 

2 . 1 

i . : 

1 . 1 

CONTFACT NO. : 19146 2 

ACTIVATIOu : CSC3C2 

BATC I : 01 c3 

COLCI.IES : 

FLATE 

C 3UNT 

U l 

btz 

74fc 

17.-

167 

ie e 

1=-1 . 

l

c

l 

CO 

. r 

DA 

c: 

p< 

AVERAGE STL DE\ 

CCUNT COUf.T 

1 7 ? . 7 7 . : 

(,-5. 7 Kl. l 

SC2.7 :3. : 

1 b 3. C lo. 7 

lib. 3 1 3•* 

lot. 3 1» -

l»-3.3 24. ? 

163. 7 27 . t5 

1 T-ACT r*c : 1?14-* 

TIV 

TCr 

.TI:N : HEPA:1 

• Dl c3 

C-3LC 

PLATE C : 

CvUi. l ' C 

2 2 2 

1 2 3 H 

164 = 

1 6 4 

l t 7 

1 3 ? 

1 2 3 

MEC 

AVERAG E 

C w U '•. T 

l'b. o 

ll=r. * 

i

c

::. 7 

15 1. 3 

174. 3 

14 7. 3 

17 3. 3 

15 4. 7 

STL OEV 

CUN T 

T 7 . ' 

t s . 1 

2 51. 2 

14. 2 

1 1 . C 

r . 6 

4:. 2 

4 6 . : 

1,/C HP 

l . C 

2.*? 

4 . 7 

1 . 3 

1 . 3 

1 . 1 

i . i 

1 . 3 

MEC 

u / C

 K,.°. ^ 

6 . 1 

146 

Reproduced Irom 

bcsl available cosy. D 
S:SOLVENT r -

:POSITIV E N : ANT HP A": I t.'l u/C S? »:T;i T COK? C L'ND i 

CGN:i514f 2 CHEHsnS STP.AIN:13 C ' LATL : :42Ce 3 ACT1V : ACT»: d 

lhCC C • 

1600. 0 

1 4 3 C C 

12C3, 

i : : 

PLCT OK AVGCCU'-TOCSE 

P 

SYriFCL I S VALUE OF SF IND 

C 3 • 

13 i c:: 

".S E ! ' *IC :\'' r -r.W 

147 
P.57 

S:SCLVENT r- = FCEITlVE N: AN THF..A•' I NT ./ C S= *:TrST CGKF-3U-.: 

C3N=l"»14r2 ChEK:HS STRAT.rl' : 0 A TF : 7 42 ;• .-3 AC T i V:C S L C 3 2 

PLOT OF AVGCOU\T*D3SE SYMPOL IS V*LUE C> SPI^O 

! 

9CCO • 

I 

• 

i 

500. 3 • P 

i 

i 

i 

! 

7C3.C • 

! 

i 

» 

! 

A C T> : C 5 

3 : c c 

& . * . • 

133.L • 

C C • 

lull. . 1 JrS 

: i r 

i.: SE : 

"issr.G VAL'J*.s : D *•:•" 

I * : 

MC^C- ?t.'-T 

C T CF t.Af. cr 

I: : 

i±s 
P.58 

S = SCLVENT P:PCSITIV E ".:ANT r>?.l». IN E W/0 3=? .:TES T C^MPCUiD 

C0N:1?14.? 2 ChE M: H S 3TK*IN:10 3 DATE::42Co 3 ACTIV:*EFA" I ACTX:3 5 

! 

2253. 3 • 

! 

i 

• 

i 

23CC C • 

; 

f 

i 

I 

175 ; y • 

1503. 3 • 

1253. : • 

I C u . L • 

75; 

53: 

PLOT OF AVGCOUNT.DCSE SYM.bOL I S VALUE OF SPIN D 

25C. C • 

C C • 

1* 1' : 

• sr I N -:CH:^--AV C 

149 
HUT ACE MC I TY-CF [• T - TA=7 

P.59 

-1 

MUTAGENICIT Y 

EXP DATE: 0421 ; 

TEST TYPE : 05 

DILUTION : : 

1IXTU1E : 

CHEMICAL : D T 

STFAIN : TA97 

PLATED : 

TECHNICIAN : OMS 

CONTRACT NO.: 

ACTIVATION: 

BATCH : D2?3 

COLONIES : 

,4ri 

SOLVENT/ 

POSITIVE 

SI 55 

PI 02 

P2 02 

DCSLEVEL 

O . C 

5 0 . 33 

ICO.wC 

1.3 3 

4.2 C 

13.: c 

43.2 0 

13CwC 

PLATE 

C C UN T 

f 2 

3fr5 

714 

121 

9

1

? 

11C 

5? 

61 

A : 

BG 

PLATE 

CCUNT 

126 

451 

138 3 

1=4 

117 

77 

0 1 

52 

i-: 

[ G 

PLATE 

CCUNT 

142 

38«* 

1C°5 

U l 

124 

94 

7 = 

41 

c: 

eo 

A'..-_r«GE 

CCUNT 

116. 7 

436. 7 

?s4. 0 

142. 3 

113. 3 

?3. 7 

7 C 7 

51. 3 

STC DEV 

COUNT 

31. 1 

3d. 4 

216. 6 

45. 3 

12. -

16. 5 

15. b 

I C C 

G/C 

A 

t 

A 

M,P 

1.0 

3. 5 

c . 3 

1.2 

1 . : 

c c . 

C 7 

C 4 

MEC 

U T ICIT Y 

EXP DATE: .'421C3 

TES1 TYPE : 35 

DILUTION: i: 2^ 

MIXTURE : 5:C 

CHEMICAL : LT 

STFAI:.: TA^-7 

PLATED : 1: ; 

TECHNICIAN : D' 

CONTFACT N'C.: 1914J-2 

ACTIVATION : CSD3C2 

LATCH : E2:3 

C'LONIES : 

SOLVENT/ 

=>OSITIVl 

D03E 

L E V i L 

= LAT " 

C0U..T HO 

PLATE 5 : 

CwU'.T L-0 

FLATE 

cuur.T 

c: AVERAGE 

cour.T 

STD CEV 

CLUf^T 

,/C Mfc 

S I 55 

^1 C4 

P2 C4 

C C 

l . C 

2.3 L 

1.3 : 

4.2 C 

1 3 . C 3 

133 C c 

1 1 3 

721 

. I C 

a 3. 

117 

57 

6 : 

1 * : • 

114 

6 1 1 

ll' J = 

C = 

124 

1 1 : 

4 9 

113 

115 

717 

127 = 

hi 

13 3 

7 7 

!-4 

114. C 

6*3 . C 

11=:. : 

1:3. c 

123. 7 

94. 7 

t i_ r 

»• * . .. 

1 . : 

62. 4 

c C 3 

23. c 

6 . 5 

16. 6 

5.tr 

2 7. 5 

i 1.0, 

A 6. 3 

A 1 C H 

: . = 

1 . 1 

w • " 

C O 

•: . 7 

* U T A G ; 

EXr-1 DATE: ! 

TEST TYPE : 

JILUTIC -. : 

•MXTU-'E : 5-

N 1 0 I T Y 

c (•••:••' ICAL : C T 

S T * A 1:. : T A = 7 

PLATE-r 1 •: 

TECHMClAN r t 

C C N T - A 2 T N 

ACTIVATIO N 

E'ATCr..* C C 

c

r

 Lo:.irr.: 

. : 1 c 14 ;• 

H •: t i ; i 

SOLVENT/ 

c s 1 T .'. v •; 

LOSE 

LEV I L 

= L A T L 

CUN T 

A: PLATE 

C3u.\T 

v . 

i- 0 

PLATE 

C3UNT 

c: A v L z»0 E 

CCN T 

S

T

? DEV 

CUN T 

u/C 

r

 1 5ri 

• • 1 3 4 

i 2 C 4 

J • _ J 

1. .1 

1.3 ? 

4 . 1 • : 

13.: : 

43.2 : 

13J.3 C 

! I'-

l l 1 :• 

112 5 

112 

1 2" 

113 

11 -

•3 1 

123 

1 ?t_2 

12 04 

14 2 

"<« 

1

 r>-

114 

1 4 ••• 

i."-2 = 

12 J- 0 

114 

12 •:-

1 3 : 

117 

. 12 7. 7 

1 : L7 . : 

1:21. 3 

122. 7 

lit. : 

1:3. 7 

i::. 7 

= C 7 

17.-. 

H C 7 

.-4 . =• 

l b . f. 

lr. 2 

? . 3 

P • 3 

1 5 . 1 

1 . 

150 
P.60 

EVJ 

S:SOLVENT F:FwSITlVE N:ANT rR AT, I Ni W/0 Sa «:TE-T CO^FGL ' 

CCN = 1914£2 CHEy.=FT ST?.MN=^7 CATr=^ 2 1 e . t ;,, v - ^ 

PLOT CF AVt-COU^WCSE SYMBOL IS VALUE CT SPIND 

• 

i 

1100. 3 • 

j 

i 

I 

13 CCC • 

!

 c 

i 

i 

i 

I 

c" 3 3.3 • 

i 

i 

! 

7 3 C 3 . 

C. f ' J » w . 

1U. i • 

.::-. : • 

l : c 3 • 

;.c • 

r: 133. 

.3sc :••• t- ICPOS* ; 

151 
P.61 

r+-HJTM*"9>'*m i iy< m .•••NTW, I "=n 

S = SCLVENT P:P3SIT1V E N:AI.THR AV IN f »/ 0 Sc *:TES T COMPOUND 

C0N:l-il4: 2 CHEM:frT STRAIN:= 7 DATE:C421r 3 ACTIV:CSD0C 2 A3TX: 

PLOT OF AVGCGUNT.DOSE SYMBOL I S VALUE CF SPIN D 

3 5 

R 

r 

V I-I 

c. 

T 

A 

\ 

T 

U 

N 

T 

15CC.C 

1400. 0 

1303. 0 

12C0.C 

11 C 0 • 3 

1030. 3 

-i-z.z 

c c : 

73C C 

6C. C 

5 c o. : 

4 C C 3 

33C 3 

2 00. 0 

13C D 

0 . 3 

• 

! 

; 

• 

; 

! 

• 

• 

! 

• 

; 

! 

4 

! 

; 

4 

! 

; 

• 

; 

; 

• 

i 

i 

• 

i 

• 

; 

; 

• 

! 

• 

• 

i 

i 

• 

i 

• 

i 

; 

• 

! 

i 

• 

IC l r0 

COSE IN M I C r 3 C. P A'.' i 

l : : : l :• •: • c 

152 
P.62 

^r*

m

*

mmv^i*»Ji**,

'.r. ••»<*? immi .inuum.wn »JJ i .K. n.ni» •JJ.I-*1"W»IHJ/J.I. I ••_I«I . iiiL^ip-ttT^wiCTjECffyyawi i-H'i i m .Jt'HjWir ^-""fumm Jgy ES6 

S = SOLvTNT P:P0S1TIVE -. :ANTHR AV J NL »/C Sc •:T:S T CC!fP3L--.'C 

C0N=l = 14c2 , CHEM = bT STP.'IN : = 7 D A T E : i. 4 2 11 3 ACT1V:HEFAC1 ACPLOT or AVGCCUNT«D0SE SYMBOL IS VALUF OF SPC D 

:35 

3 

T 

4 

\ 

T 

153C C • 

i 

; 

140C 0 • 

I 

! 

130C 3 • 

! 

; 

123C. C • 

i 

i 

11CC 3 • 

i 

; 

100 C 3 -» 

i 

; 

? C 3 • 

! 

i 

cCC C • 

733 . : • 

I 

I 

t 3 C ". •» 

; 

i 

3cc : • 

ti \. V . 

I 

I 

• 

I 

I J 

• 

I 

I 

3.3 • 

13 ir : :• 

C O E IN MJCKCOKAK'. 

153 
P.63 

m.-II.in-!. I/B..XMPIKUJ**! M M*fM*fin*P<>"*r«PR|r 

^ 

MUTAGENICITY 3F bT TA5IMUTAGENICIT Y 

EX? DATE: f4215 3 CHEMICAL : LT 

TEST TYPE: 05 STRAIN : TA98 

DILUTION : : PLATED : 

MIXTURE : TECHNICIAN : D M S 

CONTRACT 1.0.: 1914£ 2 

ACTIVATIO N : 

BATCH: D2.' 3 

COLONIES : 

SOLVENT/ 

POSITIV E 

DOSLEVEL 

PLATE 

COUNT 

A : 

BG 

PLAT; 

ecu;." 

B : 

l«G 

PLATE 

COUNT 

c : 

bC 

AVE PAGE 

CCUNT 

STD DEV 

COUNT 

C/C MS 

S I 55 

P I 0 3 

P2 33 

C. 3 0 

1.0 0 

2.3 0 

1 . 3 3 

4.2 C 

13.CC 

43.2 C 

13C.v 3 

C L 

21h 

3 4 7 

1 6 

h 

11 

14 

15 

IP 

lfl 

2 id 

19 

14 

14 

22 

13 

17 

169 

331 

l

c 

14 

15 

12 

13 

1 C 3 

1H9.C 

312. 3 

17. 7 

12. 0 

13. 3 

1 6 . C 

13. 7 

1 . 5 

2 5 . r 

31. C 

1 . 5 

3 . 5 

2 . 1 

5 . 3 

1 . 2 

l . C 

1 C 3 

17. 3 

l.C 

C 7 

0 . 7 

C

p 

3. 7 

MUTAGE N ICIT Y 

EXP DATE: :42ifc3 

TES T TYPE : 35 

DILUTION : i: 2 3 

MIXTU D E : 5."C 

CHEMICAL : R T 

STRAIN : TArri 

PLATED-: i:C 

TECHNICIAN : D^'S 

CCNTFACT N3. : J°!4H 2 

ACTIVATION : CCOCC2 

BtTCh.* 021 3 

COLONIES : 

SOLVENT / 

POSITIV E 

DOSE 

LEVEL 

FLATE 

CC UNT 

PLfl TE 

C 3 o :•.- T 

FLATE C : 

CCUNT 6 0 

AVE PAGE 

CCUNT 

STD DEV 

C:UN T 

G/C PK 

S i 5 5 

PI 04 

r i 3 4 

J . 

i . : c 

1.3 3 

4.2 : 

13 . 3 :. 

43.2 0 

.3c :o 

44 

71 ^ 

17-. ' 

1 -

1 = 

20 

12 

I t 

37 

77-' 

19 l e 

^ 2 

2 7 

21 

Ifc 

2L72t 

1731 

2 .. 

23 

17 

It. 

3 5.7 

741. 3 

17-9. 3 

21.C 

22. 7 

1-. 5 

16. 3 

1-. 0 

r . j . 

32. 2 

i:3. 9 

1 . 7 

4.51 

2 . 1 

4 . 5 

3 . 5 

1.3 

i C r-

i C 4 

C c 

C f 

C O 

*• c (. • »• 

"1 

* * «/ 

OUTAGE! . I C 

E).-- DATE: -421C 3 

TEST TTP E : CO 

DILUTIO. : : : :c 

v : x T u <• E : 5: : 

T Y 

CHEMICAL : OT 

iTri'N : TA=c 

c

-

 L A T E j : 1 :•: 

TECiiNICIA. -.: DV S 

CO NTP ACT N C 

ACTIVATION: 

HATCH : 02:3 

C3L0:1ES= 

HEF A 

,4r2 

.1 

SOLVENT / 

=JSITIV E 

D03 E 

LEVE L 

CLAT E A : 

CCUN T 3 0 

PLAT E 

COUN T 

PLAT E 

COUN T 

c : A v 0» A r, • 

b(> COU-J T 

- T? DE V 

CUNT 

i. 'C 

SI 

f 1 

F2 

55 

04 

34 

c: r 

0.5 0 

4.2 c 

13 . 3 C 

4 3.20 

1 3C 30 

23 

121-0 

ithlr 

1 = 

17 

24 

36 

141C 

215<-

2 3 

37 

2 0 

' "I 

4. C 

15 

24 

1527 

23 7 7 

25 

23 

25 

34 

•* •* •? 

c 1 . I 

13-1. 0 

2 ". 1 '• . C 

22. 3 

21.1 

2 3. 7 

2t . 7 

17. : 

7 . 2 

1*2 . E 

177 . 3 

3 . 1 

4 . : 

4 . C 

6 . 4 

1 . 7 

..C 

C 7 

1 . 2 

154 
•r ! 

uu-wiipwig^MM^^ 

S:SOLVENT FrPOSITlVE N:ANTrt3A f. I.\[ w/0 S9 »:TEST CjMPOU'jn 

CC'::l = 14fc2 ; CHEM:PT ST*AlN:9 t C T" : 04 2 1 33 ACTIV: ACT*: 

PLOT VF AVGCOU'.'T.COSE SYKPOL IS VMLU E OF SPIND 

3o 

33C ! 

33C 0 

2 7 o . C 

2 4 C 3 

211 

1 5 2 . w 

1 C .. . k 

I 

I 

• 

I 

I 

! 

• 

• 

• 

• 

* 

I 

I 

I 

6 :•. : • 

3 3.3 

c r 

1'- • I T 

L:- L T . (• ICROr-KAKi 

ir-c o 1

 r 3 0 0 

I 

i; 

i 

153 
p^m-mrmzmrz 

S:S0LVENT P:PCSITC E !v:ANTnP AM INT t/ C S9 »:TfOT CO'TOUND 

C0f.:irl4^ 2 CHEH:nT STRAp^ f 0* T r : > 21 •= 3 ACTiV:CSD:C2 ACTX:35 

PLOT OP AVOCOU .'T»[ OSE SYMBOL IS VALUE CF SPIND 

lcC C 

lfaDCO 

14 30 . 

• «. w sJ • v 

I C C 

4 (.• L . o 

L • w 

1 c i:: 

P3SL 1 ' "ICF.r-Ci.AVS 

13 0 3 ic, : 

156 
P.66 

S:S0LVEN'T P:PCSITIV E N'rANThR AM INE U/ 0 S9 «=T;S T CCNFOUNFi 

C0N = 19*4b 2 CHEK:f<T STRAIN:* ? D*TE:C421fc 2 ACTIV=HEPA? 1 ACTS = f.r, 

PLCT OF AVGCCUNT»DOSE SYMBOL I S VALUE CF SFIN D 

2250. 0 • 

200C C 

175C0 

153 

! 

• 

! 

i 

! 

; 

• 

i 

! 

! 

! 

• 

1250. 0 • 

f l 

! I 

3 

J 

"J 

T 

i c-: c 

753 . 

J k J . I • 

! 1 

CC • 

ic i: c 

HOSE I N rncp-oc.S.AMC 

. . . 4 . . 

i : : •: 

..... . 

l : c . 

157 
F'UTAC-IC I TY CF bT TA1 

MUTAGENI C 

EXP DATE: '.42 1 S3 

TEST TYPE: 05 

DILUTION : : 

•UXTURE : 

T Y 

ChLMICAL : £T 

S T F A I N : T A 1 C C 

PLATED : 

TECHNICIAN : D"S 

CC'T^AC T ' 

ACT IVATIW ' 

PATCH : 02 ; 

CCLCMES : 

c . : 19 1 4 c 2 

SOLVENT/ 

POSITIV E 

DOSE 

LEVEL 

PLATE A : 

COUNT F>G 

PLATE - : 

COUNT L-G 

PLATE C: 

COUNT PG 

AVE C A G ; 

C C UN T 

STD TEV 

CCUNT 

0 / C M 4< 

SI 55 

F l 01 

P2 01 

l . C 

2.3 0 

1.3 0 

4.2C 

13.: ; 

43.2C 

132.3 0 

164 

334 

619 

142 

121 

131 

115 

132 

4 4b 

713 

135 

126 

112 

141 

I <3 

13<-

477 

62tr 

1?1 

12 = 

I:F 

141 

94 

1-44.7 

41

 c. 3 

652.7 

132.7 

12E.3 

117. 3 

132. 3 

ofc.3 

17. 3 

75. 2 

-2. 4 

1 3 . 7 

4 . 2 

12. 3 

15. 3 

o . e 

l . C 

2.'= 

4 . 5 

MUTAGENICIT Y 

zxr DATE: :42i-3 CHE M ICAL : OT 

TEST TYPE : 05 STFiP' : TA1" 0 

DILUTION : l: 20 PLATED : I C 

1IXTUSE : 5C2 TECHNICIAN : CSOLVENT / COS E 

S 

FLATE 4 FLA' 

C3NTF.ACT .

-

. 

ACTIVATU N 

BATCH : C2 -

C'LO-ITS : 

: cc 

3 

FLAT!- C: AVEFAOF I T CEV 

POSITIV E LEVTL CCUNT H ( CCUNT '.0 CCUNT b.C COUNT C'UUT 

NP 

S I 55 

F 1 34 

F2 '-4 

tf

. U T A 0 

EXF DATE: 

TES T TYP r 

DILUTION : 

MCTU C E : 

S3LV'"JT / 

3 OS;TIV : 

S l 55 

P I 3 4 

t-2 0 4 

C C C 

i . : ? 

2 . 3 :• • 

1.3 3 

4 . 2 3 

13.: : 

4 3.21 -

1 3 C . C 

E •'. I C 

C21: 3 

: Cb 

i : 2 : 

5.. : 

DCS: 

LEVEL 

L • * *' 

c c 

1 . c 

1.3 C 

4 . 0 3 

i3.r c 

43.2 ' 

1 J t l » I '« 

1 3 -

5 7 1 

171 2 

1 3 4 

I C 

1 2 : 

1 2 7 

r. 

I T Y 

C t- E *•» 

ST° A 

r LA T 

TECr. 

CAT : A 

r: U-.T ? 

i t > 

152 7 

212 -

k c 

1 3 1 

1 1 3 

1 2 5 

114 

If. 

r. 

EC 

1 £ 3 

7 /?: 

:ziU 5 

I C 

1 1 7 

1 4 2 

0\ 

AL: HT 

: TAK : 

- 4 ** * 

-.ICIAN : D"S 

: 

l< 

PLAT E ! : 

C2UN T C 

17C 

j r 1 a 

2 0 4 3 

1 12 

1 2 1 

15C 

l i e 

i t : 

13^ 2 

14 = 7 

li>2 

Z J 

= 1 

1 1 1 

C C .TF 

ACTI V 

c ; 

C M 

PLAT E 

CCUN T 

I C 

:i4' -

21 C 

-. 3 

11 / 

1 1 •• 

135 

1 ' 7 

TCI 

. C . 

c: 

pr 

154. C 

= -9. 3 

1*7"- . 3 

U C 3 

1.7. 7 

1C9. 3 

1 2 r- . 7 

ACT ..:.: 

ATI.-V : c 

: D2 . 3 

I

r

 : = 

AVE- t. G r 

C'U. T 

1 5 s. 7 

li>. 7 

2 1 1 C 3 

1 r:.: 

123 . C 

12 C 7 

l?!-. 7 

i 1 C 3 

* "» C 

4 21.- -

2 30 . £ 

24. 3 

1 c.: 

. t z 

15. = 

c : 

19> C 

A i " 1 

STL CEV 

r. :UN T 

I C E 

: c = 

7 2 . : 

11. 5 

7 . : 

1 3 . ? 

12. -

4 . 7 

* 

~ 

ft 

i.'C 

L 

i 

: 

1 . : 

5 . " 

7 . n 

1 . 1 

v . 7 

: . 7 

3 • c 

* • r 

' 

.. c ; *•-

u : 

' • '' 

13. 3 

. , • t~! 

• • : 

• 

1 • "7* 

153 

Reproduced fre n /""" N 

bcsl «v.»ilab'e copy. ^ * J 
SrCLVLN T .^POSITIVE a =A M M t y 1N { , / c c = , = T r c T r-V'POU -

CON=1914c2 CHE'.' = C-T STRA:N=1 C LATE 04 2 1 • 3 IcTlV : *C C = C 

PLOT OF AVGCOu.'T'OOSE SYMbCL IS VALUE OF SPIND 

C

3 C C • 

! 

60C.0 • 

733, 

• 

6 u 3 . C' . 

:ic ; • 

3 

•J 

4 c : • 

Llv. l • 

IC. 

CO • 

1: i - : 

c o t iN- r-'.:rn.c<• *"->• 

159 
P.69 

S:SCLVLNT P:FO£ITIVE \ :AN THP.A » I NE ./ C Ss -:TEST C 3 ".POUND 

CGi\ = l?l4c 2 ChEK:tT ST*MN:1C C DATE:C421?3 ACT1V:CSDC2 ACT*:05 

15CC. C • 

; 

! 

14C0. G • 

; 

; 

1300. 0 * 

i 

; 

12CC 3 • 

; 

; 

113C.C • 

• 

uco. c • 

I 

I 

90C C • 

; 

i 

•.:cc • 

i 

73C 3 

f.;:. 

4 3c. : 

• k . . o • 

« 

; 

i 

1 C C G • 

i 

i 

PLOT OF AVCC0UNT«D3SE SYK5GL IS VALUE OF SPINO 

13 l r 

«.SE IN *-ICP-uFAb. 

IC 

160 
S:SCLVENT F:FOSITIV E r.-: AN T HP A v ; l.'E w / c , i, c

 »:TES T CC-MFCC " 

CO!J:1 C1 4 C, 2 ChEVrfcT STRAI N = 13C u *'TE : 3 42 1 c 3 ACT 1V:hEPA 3 I ACT1: 

P.70 

PLOT OF AVGCOUNT'COS: SYMBOL I S VALUE CF SFIN D 

! 

225C C • 

! 

! 

2 0 C 0 . C • 

1753. 3 • 

153C.C • 

133 3. 0 

.1 

!i 

11 

j i 

. * IT ' 

A -• 1 . .. 

C S E r. •,.ICF-'3?A"S 

I: 

l o l 
P.71 

MUTAGENICITY Cr IF - TA"7 

" U T A C 

EXF DATE : 

TEST TYPE 

DILUTION: 

M1XTUPE: 

SOLVENT/ 

POSITIVE 

SI 55 

PI C2 

P2 02 

E N I C 

3421£3 

: 05 

• 

DOSf 

LEVEL 

o.cc 

50.33 

IOC.C 3 

b.33 

2C6 3 

b2.6 3 

2 C 6 . L 3 

626.CO 

I T Y 

CHEMICAL : PP 

STRAI N 

PLATE D 

: TA5 7 

: 

TECHNICIAN : C^ S 

°LAT E A : 

C 0 UN T ~ G 

62 

3r3 5 

714 

9* 

153 

94 

131 

99 

PLAT E 

COUNT 

126 

451 

1 C 3 

153 

1*5 

122 

123 

I C 

f, * 

CO 

CONTh A 

AC TIVA 

tiATCF : 

COLC-NI 

PLAT E 

CCUN T 

142 

3F4 

13= 5 

l.ut 

166 

134 

132 

129 

c: 

PG 

CT NO. : 

TI C N : 

02 3 3 

ES: 

AVERAGE 

COUNT 

11C 7 

43fc.7 

904. C 

145.C 

154.7 

116.7 

12C7 

11 1.3 

1914C2 

STH DEV 

COUNT 

31.1 

3 C 4 

216.6 

47.5 

1 C S 

20.5 

4.= 

15.7 

G/C 

A 

• 

A 

MP 

1.2 

3.5 

e.3 

1.2 

1.3 

1.0 

Cl 

1.3 

M U T A G ".• I C I T Y 

Ex? D£TE: C421E3 

TEST TYPE: 35 

DILUTION: i: 23 

"IXTU'E : 5:: 

CHEMICAL: " 

STSAp.: TA97 

FLAT:*.: 13C 

TECHNICIAN: D*S 

CONTRACT NC : l«rl4f-2 

ACTIVA TI3N : cscr c 

BATCt : LC 3 

CCLGMES : 

SOLVENT/ CCEf PLATE 4 : PLATE F-: PLATE C AVERAGE STt DEV 

POSITIV E LEVEL CUN T C- COJ'.T : J 0 COUNT BC CCUNT CU\ T 

C/C MP 

? : 55 

?1 04 

F2 14 

l . C 

2.:c 

6.3 3 

2 3 . h ? 

L 2 . L C 

L. * i. » . h 

t. < a . j C 

117 

7 2 1 

1 1 C 

i i --

15 4 

1 3 3 

117 

1 2 1 

1 14 

6 1 1 

112 = 

156 

1 1 7 

1 7 2 

C c 

1 1 5 

7 1 7 

127 9 

I 4 t 

1 2 ' 

l c l 

1 3 -

1 4 -

114. 3 

it :* ss • u 

11 = '. 3 

135. : 

14 5. 3 

13 6.0 ' 

14 2. 7 

131. 7 

1 . 3 

e2. 4 

:5. 3 

2 2 . 0 

16. -

22. (i 

27. 7 

) 4 . «• 

1 . 3 

6 . C 

1 C 4 

1 . 2 

1 . ? 

1 . 2 

1 . 3 

1.2 

1 U T A 0 :• '. I C I T Y 

EXP DATE: "4215 3 CHEMICAL :

 r rTEST TYPE: C OT-AP. : T:-: 7 

CLUT K 

v

 I X T L 5 E : 

r L A T E : = 1. 3 

TECI-MCIAN : C'-S 

C •?' T r A 3 T '• ;. 

ACTIVATION : 

rATCr : DC : 

C3LCC S = 

14- 2 

. ' 1 

SOLVE'.T/ 

*»OSIT:»E 

T C E 

L E V : L 

FLAT " A : 

CCU'.T jr. 

FLATE r: CAT : c: 

C-.-,U\T ec 

.VE -.AT--

COUNT 

5 T C DEV 

C 3UNT 

/c NA 

r . t c 

- * »> ^ 

P 1 04 

P2 :•• 

k . . . 

0.5 0 

1 . fe k 

6 . 3 3 

2C6 C 

b«; . b : 

c w r . - fc 

cc: : 

112 5 

105 

= 1 

177 

12 6 

137 

1 2 3 

1-.. 2 

12 5.4 

1 7 6 

• 1 7 

1 *. w 

1 - 7 

1 2 1 

1 = 1 

14 -

i : 2 -

1 2 C 

C7. 7 

i c 7. 0 

1 2 2 i . 3 

1 = 3. 3 

12 6. 3 

17C. 7 

13 4. : 

: = o.3 

:7.- : 

4 C 7 

r4 . 9 

11. 2 

3 2. 5 

t: ^ 

1 C 4 

2 7. 0 

16-

Reproduced Irom 

bcsl .-w.-.ilabic copy. D 
S:SOLVE\ T F = POSITIV E N:ANTHF A'* INE k/ 3 3 = »:TES T C-'i'-'POU <.' 

C3N:l?14o 2 CHLr':- c STP.AIN:9 7 CATE:C421? 3 ACTIV : ACT: 

P.72 

PLOT v/F AVGCOUNT.DCSE SYM.ROL IS VALUE OF SPINO 

3 

U 

\ 

T 

! 

; 

1133. 0 • 

! 

; 

; 

1G3C C . 

! 

; 

! 

'CC C • 

rCC C • 

I 

f 

73 c : • 

! 

I • 

A -* •" * A 

4 ».•». . . • 

13C C • 

1 

! 

! 

CC • 

1 I C 

SE C rlCCt-A C 

i c : 1 -cc c 

163 
1 

T 

A 

N 

T 

S:C3LVENT PsHCSITIvE N = ANTh?A" I NT ./ f < = «:T^ T CCFGL' -.- i 

C0N:i914S2 CMEH:P? STRAIN=97 DATE:C421t3 ACT]V=CSDC3: VT*- " = 

*!" 

PLOT CF AVGCOUUTCSE SYKPOL IS VALUE CF SPP'D ' 

15; 

140C. 0 

1300. 0 • 

121 C . C 

lice : 

K v wi vi • 

, . : • 

• I J . k • 

7 C . '. • 

t 3 -• . L • 

i 

i 

| 

i 

433. 3 • 

i 

i 

2 3 c : • 

I 

J 

2 C C . C • 

I 

; 

l :• c : • ? 

I 

C C • 

13 i: •: l ? c 

c r \ .. »' T .. -. . r ••> . .. 

164 
S:SOLVtN T P:PoSITC E N:ANTHRAMN : k/v, S= • =TE ST CO.'.FCU.D '• 

CCN:l°14^i 2 CHESr^P STRAP.:9 7 DATE:'421E 3 AC TIV:HEPA3 1 ACT?:J35 

PLCT OF AVOCOU' T»DOSE SYMO-GL I S VALUE CF SPIN D ! 

1 

J 

«: 

T 

V 

T 

"i 

T 

* 

\ 

T 

-

~ 

U 

\ i 

T 

1500. 0 

140C. 0 

130C. 0 

123C C 

11CC C 

1 3 C C 3 

. m U • 4. 

^ - «• r\ 

7 %' ** • -

7GI .(. 

6 c . :• 

5 o 3 . .-

4cc : 

3 2 L . J 

233. C 

13 3. 3 

• 

; 

i 

• 

! 

i 

• 

• 

• 

; 

i 

; 

i 

4 

1 

1 

• 

1 

» 

• 

! 

i 

• 

i 

i 

4-

1 

I 

; 

• 

j 

• 

» 

• 

I 

1 c 

• 

1 

» 

13 1 C 

::sr IN MCr.ccA.".s 

165 
7—i. ,»•••. w n i wimiw.il ' 

P.75 

MUTACCIC:T Y : F FP TA9* 

MUTAGENICIT Y 

IKP DATE: :421"3 CHEMICAL : P P 

TES T TYPE : 05 STPAIV: TA98 

DILUTION: : PLATED : 

MIXTURE: TECHNICIAN: L^S 

CONTRAC T NO. : 1914J 2 

ACTIVATION: 

tATC C D2 C 

COLONIES : 

SOLVENT/ 

POSITIV E 

DOSE 

LEVEL 

PLATE A : 

CCUNT 3G 

PLATE 

COUNT LO 

PLATE 

COUNT 

c : 

PC 

AVERAGE 

COUNT 

STC DEV 

COUNT 

C /C MR MEC 

S I 

F l 

P2 

55 

03 

33 

0.3 3 

1.3 3 

2.3 C 

6.3 0 

20.6 3 

6 2 . 6 C 

2 0 C 3 0 

6 2b . 3 -3 

2 1 t 

3 4 7 

1"? 

21 

16 

14 

12 

14 

m 

2rt 

2 1 

11 

13 

15 

21 

17 

16F 

301 

l c 

13 

It. 

13 

16 

I S . 3 

1 f- 9 . C 

312. 0 

19. 3 

15. 3 

15. 3 

13. C 

It. 3 

1.5 

25.9 

31.C 

1.5 

5 . 3 

1.7 

2 . 6 

4 . 5 

A 

A 

A 

l . C 

I C I 

17. j 

1.1 

C i 

C c 

0 . 7 

C -

1 U T G E N I C I T Y 

EXP DATE: C42103 

TEST TYPE: 35 

DILUTION: l: 2C 

MXTUC : 5 C 

CHEMICAL: PP 

STRAP.-: T A 5 S 

PLATED: I C 

TECHNICIAN: D'S 

CCNTF.ACT NO. : 1914S I 

ACTIVATION : CSCCC2 

BATCH: D2 C 

COLONIES : 

SOLVENT/ 

"•OSITIVE 

COSE 

LEVEL 

PLATE 

COUNT C u 

PLATE !• : 

C2UNT rCPLATE C : COUNT BG 

Wi. Mr.r 

COUNT 

STD C 3 V 

CCUNT 

G/C KP 

01 55 

PI 04 

F2 3 4 

C C 

1 . u'.-

<:•*• -

6.3 3 

2 0 . I-- C 

t>2. 6 P 

" * <_ ' r 

C -' 3 . V •> 

L 2 o . c : 

44 

71 = 

17c * 

32 

2 2 

17 

2 6 

2 1 

' 7 

7 7 -

1 9 C 

26 

o •» 

2 4 

13 

7 2 o 

17( 1 

17 

23 

13 

3 5. 7 

74 1. ? 

7 = 9. 3 

4 2 . C 

19. 7 

23. 0 

21. 3 

i <•'. 7 

9 . 1 

32. 2 

133. 9 

22. 7 

2 . c. 

6 . 3 

7 . r 

4 . 9 

l . C 

:L' .

 r 

; C 4 

1 . 2 

C . 6 

C f 

C C 

U T A (. r <., i c I T Y 

EXP DATE :

 -

TEST TY? C 

DiLUTIC -. : 

"1XTUFE= 5 

421 a0 

r 5 

i : c 

CutiMIC'L : = F 

STRAIN : TA93 

FLATE3 : 13 3 

TECHNICIAN : D"3 

C TFACT ': 

AC T I VATI C 

•?ATCi-: 02 1 

C CONIES : 

•.: 1914' 

H E r A - 1 

SOLVENT/ 

POSITIVL 

COSE 

LEVEL 

FLATE 

COUNT 

A : 

3 0 

PLnr - : 

CCf. T C 

PLATE C: 

COUNT PG 

AVE -'AO: 

CCUNT 

S T : CEV 

CUN T 

3 1 55 

I- 1 04 

P 2 3 4 

0 . :• •: 

0.5 C 

1. c 

6 . 3 3 

2 C 6 2 

6 2 . fat 

2 C t . C 

6 2 D . .' . 

2 3 

1 2 C 

1^19 

27 

27 

2 3 

23 

fc7 

141 3 

215 * 

23 

: o 

14 

24 

1L2 7 

2'7 7 

-> i <. *. 

If2

 : 

3 : 

2 '• : 

c c : 

2: I f. c 

24. 3 

1 C 7 

21. 7 

2 =. 3 

"> = .

 r

. 

\o.: 

I C E 

17 7.3: 

2 . 3 

6 . 4 

7 . 1 

6 . : 

2 . 0 

7 . 7 

-•1 

c * 

(.1 

I.) 

c q, 

166 

Reproduced Irom 

best available copy. 
• u ' m • ">"" v* v—'w -*-•'..i v*> '>w'i .m, w w i ^w^iiwi^iw^w»wptpt«wwp^^w«**»w>wyiw«WBff»ogwi>^w^w.wv^W 

S:SCLVENT P:POSITIVE ' :ANTH« A" INE w/3 35 • = TE 3T CT'POUJ D 

CCN:l s14? 2 CHEM.:?P STRAIN: 1*;- DATL':0421?3 ACTIV: ACTXrOj 

PLOT CF AVGC0U'T«D3SE SYMBOL IS VALUE OF SPIND 

330.C 

330. 0 

270. 

24G. : 

21CC 

i 

ii 

!| 

• i 11 

1 C . C 

.51 . 3 

C C C • 

Ob.. . 

0. 3 

i 

• 

i 

I 

i 

• 

i 

i 

i 

• 

i 

; < 

i 

• 

io i - : 

C S E 1'. MCn- L = A!--0 

133 1 :: :: 

167 
P.77 

3 

U 

N 

T 

•gHwi—a .m-jjii u i .11 11 .i np.iiM.iwnmpnii.nij u. ii, W i W -m W ,)yii i ,, j. JL| , iWJi)lvB!iUa|M<| .mjumnwmwi IUIW.UH-IWW 

S:SOLVENT P:P0S1TIVE N :A NThF. AM P.'E W/C C *:T"ST C<" vPOl'--

C0":l914; 2 CHEM:PP STRAIN=',£ CATE:3421f3 A CT I V =CS 0 C 2 ' ' l 

PLCT OF AVCCOU .T*D0SE SYF.D3L IS VALUE '. F iPpj; ; 

1033. C • 

1633.3 • 

! 

1410.0 • 

123C.3 • 

ice : • 

c c : • 

bi<t . . • 

4 : c . :• • 

u • 

L . : • 

:

r

 1 *• c. 

DCE il. MICCfAViic: : I C 

168 
!i .u\t,tji^mBUBm^m^i*m\K.v\),jm^ MWBJ,' ' LW w-i.-^wyyew 

S:SOLV"NT P:F0SITIVE N:ANTHRAMHr »/G S9 «:TrST CCI-iFCU.iP 

C0N:1914F2 CHEM:PP STRAlN:9o DATE:342155 ACTiV:H£.PACl ACTX:35 

PLOT OF AVOCOU JT«D'S E SYMO-CL IS VALUE OF SPPID 

225C.C • 

2000. 0 • 

! 

i 

! 

1753.3 • 

1 — V - . k • 

I 

! 

! 

; 

125..r • 

10 3 0. 3 • 

50C. 

250. 

0.0 • S 

13 i:: . l:: c 

r. C E IN CCP C KAf'S 

169 
<1 U T A 0 

E XP DATE : 

TES T TYPE : 

DILUTION : 

FIXTURE : 

SOLVENT / 

POSITIV E 

E N I C 

3421*3 

05 

COSE 

LEVEL 

MUTAGENICITY OF FP TA1 C 

T Y 

ChEMICAL: PP 

STRAIN : T A I o •: 

PLATED : 

TECHNICIAN : D" S 

FLAT E 

C 0 UN T 

A: PLAT E 

COU-iT 

CONTRAC T NO. 

ACTIVATION : 

RATC : 02C3 

CCLCMfS : 

lV14f2 

PLATE 

COUNT 

c : 

PG 

AVERAGE 

COUNT 

iTD DEV 

COUNT 

G/C ME: 

SI 

PI 

P2 

55 

91 

31 

C t w o 

2 . 30 

6.3 3 

20.6 0 

6 2 . t>C 

2UC0 C 

62t.u 3 

164 

334 

h i ? 

123 

141 

93 

? t 

123 

132 

446 

713 

129 

i *> v 

136 

= f. 

13P 

477 

626 

132 

136 

124 

79 

115 

14-..7 

419.C 

652. 7 

127.C 

135. 7 

117-7 

9CC 

112. 3 

17. 0 

75. 2 

52. 4 

6 . 2 

5 . 5 

22. 2 

10.4 

32. 2 

1.2 

2 . = 

4 . 5 

0 . 9 

C ? 

0.? 

C.6 

3 . * 

MUTAGE N IC I T Y 

IK? DATE: '.421C 3 

TEST TYPE : 

DILUTION: C 23 

MIXTUFE: 530 

CH'lICiL : PP 

STRAIN : TAI C 

FLATE:- : I:; 

TECHNICIAN : D-< S 

CCVT 9ACT «\C 

ACTIVATION : 

BATCH : 02:3 

COL.. H.S: 

CO 

L9146" 

.•"Li 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

FLATE A: 

COUNT OO 

FLATE 

COUNT 

FLATE 

COU.-.T 

c: 

f 0 

AVLFAC r 

COUN T 

S T D Df V 

C JUN T 

0/C MR 

SI 55 

PI 04 

P2 40 

w • «* U 

1. c 

2. C 

6.3: 

2 C G 0 

e2.63 

2 C t. j C 

b26.C 

1 3 r. 

571 

7 12 

11C 

13b 

126 

101 

141 

1 1 * 

1 13 

1 12 

1 41 

154 

161 

l'jbi 

'.4 A 7 

o ' 

74 

73 

173 

16i 

154.C 

1479.3 

1 C 7 

1 0 f. 7 

IC 3.7 

i 5 r . 3 

154. 3 

1 3. * 

421. 9 

*-. * • 

c . . ^ 

31. 1 

27.5 

16.2 

13.5 

C 7 

C 7 

3.7 

c: 

1 .3 

MUTAGENICIT Y 

EXF DATE : :42ic 3 CHEMCAL : PF 

TES T TYPf.: 

DILUTION : l: 

MIXTURE : 5 C 

S T f. A I !. 

PLATE : 

T r r f. N I 

T A 1 

1 - -

cs 

CONTRAC T ';;.: "91 

ACTIVATION : HERA' 

BATCr.: DO C 

cLO-.: i s= 

452 

1 

SOLVENT / 

p 3 s 1 T : v -: 

51 55 

PI 3 4 

C C4 

0C3L 

L c. V I L 

C . 3 3 

0.53 

1.03 

6.3? 

2 0 . t 0 

62.6 0 

2 3 f . •: c 

626.:c 

i-LATE A : 

c:u.T tk. 

14r 

1527 

2 i : = 

144 

14 5 

12 3 

1 4* 

123 

PLATE 

Cd.T 

17C 

153S 

2342 

135 

1 11 

157 

119 

11° 

PLATE 

CCUNT 

i t : 

114 -

2 1 r- •: 

'< (' 

1 

c r 

c: 

bC 

AVERAGE 

CCN T 

15C 7 

1434. 7 

2119. 3 

142. 7 

114. 3 

13 2. 7 

117. C 

11 C C 

STC LEV 

C ..ui.T 

13. 5 

2 21.5-

72. 3 

b . l 

29. 1 

21. 1 

26. 1 

19. 2 

1 . : 

r • -

13. 3 

3.7 

v • ^ 

C 7 

. C 7 

Reproduced 

besl o«a" 

irom 

able copy ID 

i 7n 
P.80 

S:SOLVENT p:POSITIVL N=ANT H3AM. 1NE U/0 Ss «=TE ST CCI-.POU..T; 

191482 CHEM:pP STRAC:1* C LATE:C421t 3 AC TI V : AC" 

PLOT OF AV3C3UWT*CJSE SY*bCL IS VALUE OF SPI C 

="00.0 

800. 0 

7GC. 

I 3 C 5 • 

b 3 i 

4 : c : • 

3 C C • 

v. 

103. 0 • 

O.C • 

13 I C 

DOSE IN I--1 CROC-RAM 3 

1 7 1 

( J 

! 1 

K

, 

' 

I , 

i 

J 
P.81 

T i 

S:SCLvEN T F:F -:-SIT:V f N : AN T HP. A* I N f ,/ 3 S? «:TLS T C3KP0U. D 

C0N=1?14* 2 CHEH:p r STRA P =1 CO E A Tf : ?4 2 1 '-3 AC T I V:C SDO L 2 ACT*: 

PLOT OF AVCCOUNT.LDiE SYMBOL I S VALUE OF S»iN : 

u 

! 

150 0.C • 

; 

; 

I 4 C 0 . u • 

; 

; 

130C P • 

; 

; 

1200. 3 • 

i 

; 

II C. 3 • 

; 

i 

1000. C . 

I 

I 

. U w . . 4-

I 

I 

C C C • 

i 

! 

7 ? c :• » 

i 

6 0 3. 3 • 

j 

i 

4 0 C 0 

203. 3 • 

I c 

I 

I C C 3 • 

i 

• 

C . 3 • 

- • • 

lf 1" I 

DOS: IN * iCrC . 

172 

» .J 
S:OCLVENT

 e :f'lSIUV E I. =A N T t-R A»- p.--; fc/L <,= .rT'O T C'FCir, -

C0N:l914o 2 CHEM:FP STRAP.:13C i A TE =

 r *? 1-

-3 A C T I V :r-.EPA .' 1 ' 

• c c = 

PLOT OF AVGCOUNT.C03E SYKPO L IS VALUE CF SPIND 

2253.C • 

203C 3 • 

1753. 0 • 

1 5 C C ', • 

1 2 ; 

U 

N 

T 

1 C . 

753. 3 • 

b 3 C 3 • 

250. : 4 

c : • 

I : : i

r 

Dk-SE IN MCKGGRA.V 

173 
MUTAGENICIT Y CF AC - TA9 7 

MUTAGENICIT Y 

E X P DATE : 04276 3 CHEMICAL : A C 

TES T TYPE : 05 STRAIN : TA9 7 

DILUTION : : PLATED : 

MIXTURE : TECHNICIAN : LG M 

CONTPAC T NO.; 

ACTIVATION : 

BATCH: D263 

COLONIES: 

191482 

SOLVENT/ 

POSITIVE 

SI 57 

PI 02 

P2 02 

DCSE 

LEVEL 

5 0 . 00 

10C3 C 

0.3C 

l.CC 

3 . 0 0 

I C C 

31.0 0 

PLATE 

COUNT 

133 

31S 

705 

96 

106 

126 

99 

79 

A : 

3G 

PLATE 

COUNT 

151 

271 

5 4 9 

111 

121 

1 3 6 

83 

97 

B: 

BG 

PLATE 

COUNT 

142 

264 

5C-1 

e4 

92 

67 

91 

129 

c : 

BG 

AVERAGE 

COUNT 

142. 0 

264. 3 

535 . G 

97. 0 

106. 3 

106. 3 

9C.0 

131. 7 

STD DEV 

COUNT 

9 . 0 

29. 4 

136. 7 

13. 5 

14. 5 

15. 5 

5 . 5 

25. 3 

C/C MR MEC 

1 .0 

2 . 0 

4 . 1 

0 . 7 

0 . 7 

0 . 7 

O.b 

0 . 7 

MUTAGENICIT Y 

ZXP DATE: 342763 

TEST TYPE: C5 

DILUTION: K 20 

MIXTURE: 5CC 

CHEKICAL : 

STRAIN : TA 

PLATED : 13 

TECHNICIANAC 

97 

LGM 

CONTPACT NG.: 191482 

ACTIVATION: CSCC 2 

BATCH: D263 

COLONIES: 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE A: 

CCUNT 30 

PLATE B: 

COUNT BG 

PLATE 

COUNT 

c: 

BC 

AVERAGE 

COUNT 

STD DEV 

COUNT 

G/C MR KEC 

SI 57 

PI C4 

P2 if 

o.co 

1.0C 

2.30 

0.30 

1.C0 

3.30 

10.30 

31.00 

155 

572 

64C 

139 

141 

162 

127 

154 

1?5 

436 

865 

125 

131 

114 

152 

117 

162 

460 

763 

145 

134 

15? 

136 

123 

167. 3 

479. 3 

322. 7 

13b. 3 

135. 3 

144. 7 

136. 3 

131. 3 

15. 7 

?4. 7 

53. 2 

10. 3 

5 . 1 

26. 6 

12. 7 

19. 9 

A 

A 

A 

1.3 

2 . ° 

4 . 9 

0 . 8 

0 .6 

0 . 9 

0 . 6 

O.t: 

MUTAGENICIT Y 

EXP DATE: C427c3 

TEST TYPE: 05 

DILUTION: l : 20 

MIXTURE: 5 C 

CHEMICAL : AC 

STRAIN : TA9 7 

PLATED : IC O 

TECHNICIAN : LG M 

CONTRACT NC. : 1914S2 

ACTIVATION : HEPACI 

BATCH : D2b 3 

COLONIES : 

SOLVENT / 

POSITIV E 

DOS E 

LEVE L 

FLAT E 

COUN T 

A : 

BG 

PLATE !? : 

COUNT fc-G 

PLATE c: 

COUNT EG 

AVERAGE 

COUNT 

STD DEV 

COUNT 

G/C MR MEC 

SI 57 

PI 04 

P2 34 

0.33 

0.50 

l.C? 

0.3G 

1.0 3 

3 • 0 C 

io.c; 

31.00 

123 

731 

1027 

137 

169 

66 

124 

140 

19S 

676 

653 

132 

119 

12« 

121 

118 

171 

644 

761 

13° 

161 

15b 

120 

117 

154 . C 

b84. 3 

663. 3 

136. 3 

149. 7 

123. 3 

121. 7 

125.C 

13. 5 

43. 8 

135. 1 

3 . 6 

26. 9 

34. 8 

2 . 1 

13. 0 

l . C 

3 . 7 

4 . a 

0 . 7 

0 .6 

0 . 7 

0 . 7 

C.7 

174 

•i 

J 
S:SOLVENT P:POSITIVE N:ANTHRA»INE U/0 S5 *=TEST COMPOUND 

C0N:191482 CHEM:AC STRAIN:97 - DATE:042783 ACTIV: ACT*£:Cb 

PLOT OF AVGCOUNT.OOSE SYMBOL IS VALUE OF SPIND 

c 00.0 

800.0 

700.C 

6 C C« 0 

5CC, 

I 

0 

J 

\ 

T 

4&C.0 

3 " n ' 

200.0 

luu* J 

0.0 • 

io i re 

DOSE IN MICROGRAM S 

ioc: 1CCC 0 

175 

.-• J 
S:SOLVENT P:P0SITIVE N:ANT HR-4M INE U/0 S9 *=TEST COMPOUND 

CON:l91482 CHEM:AC STRAIN:97 DATE:C42783 ACTIV:CSDC02 ACTX : 0 5 

PLOT CF AVGC0UNT*D0SE SYMBOL IS VALUE OF SPINO 

* I.I #* 

0 

J 

N 

T 

900. 0 

600. 0 

700. 0 

600. 3 

500. 0 

400.C 

333. 0 

23G.C 

100. 0 

C O 

• 

• 

• 

• 

! S 

• 

• 

10 ICC 

COSE IN MICROGRAMS 

1CCC 1003 0 

176 
S:SOLVENT P:POSITIVE N:ANTHR A-" INE U/0 S9 »:TEST COMPOUND 

C0N:191432 CH£M:AC STRAIN:97 DATE:0427S3 ACTIV:HEPA01 ACTX:P5 

PLOT OF AVGCOUNT-DDSE SYMBOL IS VALUE OF SPIND 

n 

900. 0 

! I 

R 

r 

V 

r 

R 

T 

A 

N 

T 

800. 0 • 

t 

! 

I 

700.C • 

! 

! 

I 

; 

6CC.3 • 

! 

! 

50C C • 

400. C • 

300. 3 

200. 3 

1CC. 0 

i 

0 . 0 • 

10 133 

DOSE IN MCROGF.AMS 

lCC i 1 3 C 2 0 

177 
MUTAGENICITY CF AC - TA98 

MUTAGENICIT Y 

EXP DATE: 342783 CHEMICAL: AC 

TEST TYPE: 05 STRAIN: TA98 

DILUTION: : PLATED: 

MIXTURE: TECHNICIAN: LGM 

CONTRAC T NO . 

ACTIVATION : 

BATCH: D263 

COLONIES: 

191462 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLffTE A : 

COUNT 6G 

PLATE e: 

COUNT BG 

PLATE C: 

COUNT BG 

AVERAGE 

CCUNT 

STD DEV 

COUNT 

O/C MR MEC 

SI 

PI 

P2 

57 

03 

03 

C.GO 

1.00 

2.00 

0.30 

l.CC 

O • J <j 

10. OC 

31.00 

22 

667 

186 

19 

37 

18 

37 

23 

33 

774 

149 

22 

26 

14 

26 

18 

22 

883 

175 

23 

2C 

23 

20 

25 

25. 7 

774. 7 

17C.0 

21. 3 

27. 7 

17. 3 

27. 7 

23. 3 

6 . 4 

108. 0 

19. 0 

2 . 1 

6 . 6 

3 . 1 

8 . 6 

5 . 5 

1.0 

30.I j 

6 . 6 

0 • % j 

1.1 

0 . 7 

1 . 1 

C 9 ! 

MUTAGENICIT Y 

E X P DATE : 3427t 3 

TEST TYPE : 05 

DILUTION: i: 20 

MIXTURE: 53C 

CHEMICAL : AC 

STRAIN : TA? « 

PLATED: 1C3 

TECHNICIAN: LC 

CONTRACT NO.: 151462 

ACTIVATION: CSD232 

BATCH: £263 

COLONIES: 

SOLVENT/ 

POSITIVE 

SI 57 

PI 04 

F2 C4 

DOSE 

LEVEL 

l.CC 

2.3 3 

G.30 

1. C 3 

3.0C 

10.CO 

31.OC 

M U T A C E N I 

EXP DATE: 342763 

TES T TYPE : 05 

DILUTION: i: 20 

MIXTURE: 5 '. 0 

SOLVENT/ 

POSITIVE 

SI 57 

p-1 04 

P2 C4 

DOSE 

LEVEL 

0 • v c 

C 5 3 

1.3? 

0.33 

1.0 0 

3.00 

10.30 

31. 33 

PLATE 

COUNT 

25 

42-

959 

26 

25 

23 

34 

33 

A : PLATE 

COUNT 

42 

399 

1024 

43 

25 

1" 

33 

39 

PLATE C: 

COUNT BC 

36 

397 

8 C 

19 

31 

?

e 

24 

32 

AVERANE 

COUNT 

34. 3 

405. 0 

T3C 3 

2 C 3 

27. 0 

23. 3 

29. 3 

34. 7 

STO DEV 

CCUNT 

6 . 6 

21. 6 

UO. 5 

12. 3 

3 . 5 

4 . 5 

5.C 

3 . 3 

CIT Y 

CHEMICAL : AC 

STRAIN : TA9 6 

PLATED: 100 

TECHNICIAN: LGM 

FLATE A: PLATE IC 

COUNT BG COUNT E CCONTFACT NC. 

ACTIVATION : 

BATCH: 0263 

COLONIES: 

: 191482 

HE FAC1 

37 

711 

1 294 

33 

37 

40 

23 

25 

0 ( 0 

1333 

38 

26 

2 9 

ie 

26 

PLAT E 

COUN T 

5 3 

754 

1062 

37 

32 

2 2 

29 

30 

c: 

BC 

AVLPAGE 

COUNT 

3 C 3 

756. 0 

1159. 7 

3 6. C 

31. 7 

3 C.3 

23. 3 

2 9. 0 

STD DEV 

COUNT 

14. 0 

4 9 . 1 

124. 3 

2 . 0 

5 . 5 

9 . 1 

5 . 5 

1.0 

G/C MR 

A 

A 

A 

1 . 0 

11.3 ! 

27.Ii 

0.9 ! 

o.e , 

0.7 ] 

0.° i 

l.C : 

G/C MR 

1.0 

1 C 6 

30. 3 

C 9 

0 . 8 

O.P. 

0.6 ' 

o.e 

MEC 

MEC 

178 
S:SOLVENT P:P0SITIVE N:ANTHRAMINE W/0 Sc

 «:TEST COMPOUND 

C0N:1914e2 CHEM:AC STRAIN = 98 DATE:C42763 ACTIV: ACTX:'G5 

900. 0 

0 

u 

N 

T 

700. 0 

630. 0 

830. 0 • 

! 

! 

! 

• 

• 

! 

! 

j 

! 

• 

! 

i 

! 

! 

5 0C.C •» 

i 

! 

; 

; 

• 

t 

; 

! 

i 

3 3 C 0 • 

43C 3 

! 

23C C • 

i 

i 

PLOT OF AVGCOUNT'DOSE SYMBOL IS VALUE OF SPIND 

10C 0 • 

! S 

6.0 • 

10 1?0 

DOSE IN MICROGRAMS 

100 0 10000 

179 
.iW^'W w^mfMmm^i 1 

S:SOLVENT P=PCSITIV E N:ANTHRAMINE w/ 0 S9 <-:TEST COMPOUND 

C0N:i?14a 2 CHEM:AC STRAIN:9 8 DATE=C4276 3 ACTIV:CS000 2 ACTX=3 5 

PLOT OF AVGCOuNT.DOSE SYMBOL I S VALUE OF SPIN D 

! 

; 

110C. 0 . 

I 

! 

0 

U 

T 

1000. 0 • 

! 

• 

i 

900. 0 • 

t 

; 

800. 0 * 

7D0. 5 • 

! 

! 

; 

6CCG • 

t 

i 

i 

500. 0 • 

t 

1 

i 

4CC.C • 

30C C •» 

203. 0 • 

100. 0 • 

0 . 0 • 

s * 

10 1 2 C 

DOSE I N MICROGRAMS 

100 0 

i » - - 4 -

100C0 

180 
p*-*"' 

«: 

T 

V 

r 

* 

T 

A 

N 

T 

0 

U 

N 

T 

S : 

C0N:191432 

• 

1500.C • 

; 

I 

140C 3 . 

i 

; 

1303.C • 

! 

! 

1200. 3 • 

! 

; 

1100. 0 . 

! 

! 

13CC.C • 

! 

• 

503. 0 • 

i 

! 

POC.C • 

• 

1 

70C 3 • 

t 

; 

6 C C 3 • 

i 

i 

530. 0 • 

• 

4 w «. . k • 

" 1 ' 1 

" "' 

P.90 

SOLVENT P:POSITIV E N:ANTHRAMINE W/0 S9 «:TES T COMPOUNO ' 

CHEM:AC STRAIN:9 8 DATE:C4278 3 ACT1V:HEPA0 1 ACTX:0t1 j 

PLOT OF AVGCOUNT*DOSE ?YMBOL I S VALUE OF SPIN D | 

l 

1 

i 

t 

i ! 

i 

1 

i j 

i 

i ! 

i ! 

p ; 

• 

• 

i 

« 

i

 : 

' 

p 

;

 I 1 

i 

: t 

i 

i 

i 

I 

3 03.C • 

! 

• 

2 C C C • 

j 

; 

C O • 

; 

C O • 

13 

13 ICO 

DOSE I N KICROGRAMS 

181 

100 3 1 0 CC 0 
P.91 

W^W^SI^^^f !|L»ll|JLI».yillJ.^'*U.''UIH'j«tUliP»IH^ 

MUTAGENICITY OF AC - TAlOO 

M U T A G 

E X P DATE : 

TES T TYPE: 

DILUTION: 

MIXTURE: 

SOLVENT/ 

POSITIVE 

E N I : 

342783 

05 

T Y 

CHEMICAL : AC 

STRAIN : TAIO O 

PLATED: 

TECHNICIAN: LGM 

SI 

PI 

P2 

57 

01 

01 

DOSE 

LEVEL 

o.oc 

l.CC 

2.00 

0.30 

l.CO 

3.0C 

10.GO 

31.00 

PLATE 

COUNT 

178 

585 

769 

154 

167 

144 

150 

152 

A : 

BG 

PLAT E e: 

COUNT BG 

197 

548 

B13 

139 

148 

155 

16u 

249 

MUTAGENI C 

EXP DATE: 342783 

TEST TYPE: 05 

DILUTION: l: 20 

MIXTURE: 530 

I T Y 

CHEMICAL : AC 

STRAIN : TA103 

PLATED: 130 

TECHNICIAN: 

SOLVENT/ 

POSITIVE 

SI 

PI 

P2 

57 

04 

04 

DOSE 

LEVEL 

o.co 

1.0 0 

2.00 

0.30 

1.33 

3.00 

10.00 

31.00 

PLATE 

COUNT 

164 

886 

1603 

127 

124 

97 

122 

12 8 

A: 

BG 

LGM 

PLATE- 9: 

COUNT BG 

181 

813 

1759 

131 

171 

142 

129 

133 

MUTAGE. N ICIT Y 

EXP DATE: 342783 

TEST TYPE: 05 

DILUTION: l: 2 0 

MIXTURE: 530 

CHEMICAL : AC 

STKAIN : TA10 3 

PLATED: 130 

TECHNICIAN: LGM 

SOLVENT/ 

POSITIVE 

SI 

Fl 

92 

57 

04 

34 

DOSE . 

LEVEL 

C O O 

0.5? 

l.CC 

0.30 

1.0 0 

3.00 

10.30 

3C0 C 

PLATE 

COUNT 

216 

1016 

18F6 

155 

132 

153 

132 

146 

A: 

BG 

PLATE B: 

COUNT DG 

876 

1765 

165 

196 

146 

156 

164 

CONTRAC T NO. : 19148 2 

ACTIVATION : 

BATCH: D263 

COLONIES: 

PLATE 

CCUNT 

179 

598 

674 

170 

149 

137 

163 

240 

c: 

BG 

AVERAGE 

COUNT 

164.7 

577.0 

752.0 

154.3 

154.7 

145.3 

166.3 

227.0 

STD DEV 

COUNT 

10.7 

25.9 

71.0 

15.5 

10.7 

5.1 

18.2 

30.6 

CONTRAC T NO. : 15146 2 

ACTIVATION : CSDCC 2 

BATCH : 0263 

CCLCMES: 

PLATE 

COUNT 

177 

853 

1679 

134 

178 

115 

131 

127 

c: 

BG 

AVERAGE 

COUNT 

174. 0 

664.7 

1660.3 

13C.7 

157.7 

119.0 

127.3 

129.3 

STO DEV 

CCUNT 

5 . 9 

47. 7 

78. 0 

3 . 5 

29. 4 

22. 5 

4 . 7 

3 . 2 

CONTRACT NO. : 191482 

ACTIVATION*. HE' AC1 

BATCH: D?d3 

COLON!EJPLATE c : 

COUNT BG 

153 

1121 

1773 

159 

153 

146 

156 

122 

AVERAGE 

COUNT 

199. 7 

997. 7 

1R3S.C 

167. 7 

CO. 3 

14 9.0 

14S.0 

144. 7 

STD DEV 

CCUNT 

14. 2 

113. 6 

67. 7 

15. 0 

32. 6 

3 . 6 

13. 9 

21. 2 

G / C MR 

A 

A 

A 

1.0 

3 . 1 

4 . 1 

0 . 3 

0. 8 

0.8 

0 . 9 

1.2 

G/C MR 

1.0 

5 . 0 

9 . 7 

0 . 3 

0 . 9 

0 . 7 

0 .7 

0 . 7 

• /C MR 

5.C 

9 . 1 

O.c 

u . 6 

0.7 

0.7 

0 . 7 

i • 

MEC 

MEC 

MEC 

182 
*m\u.m.9)*irBtf3Pmie*mmm

tt&m!mi 

mv ,o?:!SLVE J

T P=P0SITIV E

 ^=»NTHRAMINE W/C S9 .=TEST COMPOUND 

C0N=191482 CHEK=AC STRAIN=100 DATE=042783 ACTIV= ACTX = 05 

0 

u 

N 

T 

! 

• 

! 

• 

I 

! 

• 

! 

! 

! 

! 

• 

! 

; 

; 

• 

• 

i 

! 

f 

• 

; 

; 

! 

f 

• 

i 

i 

! 

; 

• 

! 

; 

i 

i 

• 

; 

f 

i 

! 

1C0.0 • 

{ 

• 

I 

! 

• 

9U0.3 

600 . 0 

700. 0 

600. 0 

500. 0 

4CC ; 

303. 0 

200. 0 

PLOT OF AVGCOUNT.OOSE SYMBOL IS VALUE OF SPINO 

0.3 

10 ICO 

DOSE IN MICRCGPAM S 

1330 10 0 20 
P.93 

S:SOLVENT P=PCSITIVE N:ANTtl«M-INE «* * S9 «:T"ST COMPOUND 

C0N=19l4b2 CHEM=AC STRAIN=1C0 DATE**C42Jb3 ACTIV=CSD002 ACTX=05 

D 

U 

<J 

T 

1800. 0 . 

i 

! 

! 

! 

1600. 0 • 

! 

i 

! 

; 

1400, 0 • 

f 

I 

I 

! 

1200. 0 • 

! 

; 

! 

! 

1CG. G • 

; 

; 

; 

i 

63C C • 

! 

; 

; 

; 

600. 0 • 

; 

; 

i 

! 

' 40C 0 • 

I 

I 

! 

t 

i 

! 

O.C • 

PLCT OF AVGCOUNT.DO';'. SYM60L IS VALUE OF SPIND 

if ir e 

DOSE IN MICROGRAMS 

1000 10030 

184 
P.94 

403.C 

200.0 

0.0 • 

10 ICC 

DOSE IN MICROGRAMS 

1000 1C0C0 

S 
P.95 

MUTAGENICITY GF EO TA9 7 

MUTAGENI C 

EXP DATE: 04278 3 

TEST TYPE: 05 

DILUTION : : 

MIXTURE : 

T Y 

CHEMICAL : EO 

STRAIN : TA9 7 

PLATED: 

TECHNICIAN: LGM 

SOLVENT/ 

POSITIVE 

SI 

PI 

P2 

55 

02 

02 

M U T A G 

EXP DATE : 

TEST TYPE : 

DILUTION: 

MIXTURE: 500 

DOSE 

LEVEL 

0.G0 

50.00 

100.00 

1.00 

3.00 

11.00 

37.00 

111.03 

E N I C 

042783 

05 

i: 20 

PLAT E 

COUNT 

127 

31« 

7?5 

121 

136 

135 

98 

lie 

A: 

BG 

PLATE 

COUNT 

150 

271 

549 

145 

124 

150 

97 

124 

B : 

BG 

T Y 

CHEMICAL : EO 

STRAIN : TA97 

PLATED: 100 

TECHNICIAN: LGM 

SOLVENT/ 

POSITIVE 

SI 

Fl 

P2 

55 

04 

04 

OOSE 

LEVEL 

0.03 

1.00 

2.33 

1.00 

3 . O-C 

11.00 

37.30 

111.00 

»LATE A: 

COUNT BG 

136 

572 

84 3 

142 

112 

132 

169 

164 

PLAT E e: 

COUNT HG 

163 

4C6 

865 

139 

157 

158 

155 

163 

MUTAGENICIT Y 

EXP DATE: 042763 

TEST TYPE : 05 

DILUTION: l: 20 

MIXTURE: 530 

CHEMICAL : EO 

STRAIN : TA9 7 

PLATED: 100 

TECHNICIAN: LGM 

SOLVENT/ 

POSITIVE 

SI 

PI 

P2 

55 

04 

0 4 

DOS E 

LEVE L 

0.50 

l.CC 

1.00 

3.0 0 

11.3 0 

37.30 

111.00 

PLATE 

COUNT 

15S 

731 

1027 

154 

151 

153 

147 

141 

A: 

BG 

PLATE 

COUNT 

143 

673 

853 

151 

160 

146 

157 

167 

&: 

BG 

CONTRACT NO.: 

ACtlVATION.* 

BATCH: D263 

COLONIES: 

191482 

PLATE 

COUNT 

169 

264 

501 

154 

164 

134 

1C6 

116 

c: 

BG 

AVERAGE 

COUNT 

148. 7 

2e4. 3 

585. 0 

140. 0 

141. 3 

139. 7 

130. 3 

119. 3 

STD DEV 

COUNT 

21. 0 

29. 4 

106. 7 

17. 1 

20. 5 

9 . 0 

4 . 9 

4 . 2 

CONTRACT NO.: 1914e2 

ACTIVATION: CSD0C2 

BATCH: D263 

COLONIES: 

PLATE C: 

COUNT BG 

17C 

<!6 0 

763 

162 

176 

155 

149 

168 

AVERAGE 

COUNT 

156.3 

475.3 

822.7 

147.7 

148.3 

148.3 

157.7 

165.0 

STD DEV 

COUNT 

15.0 

84.7 

53.2 

12.5 

32.9 

14.2 

10.3 

2.6 

CONTRAC T NO. 

ACTIVATION : 

BATCH: D263 

COLONIES: 

: 191462 

HEPAC1 

PLATE 

COUNT 

145 

644 

761 

155 

167 

177 

173 

142 

186 

c: 

RG 

AVERAG E 

COUN T 

150. 0 

684. 3 

863. 3 

163. 3 

159. 3 

158. 7 

159. 0 

150. 0 

STD OEV 

COUNT 

7 . 5 

43. 6 

135. 1 

18. 3 

8 . 0 

16. 3 

13. 1 

14. 7 

G/C MR 

A 

NAA 

1.0 

1.9 

3 . ; 

0 . 9 

1.0 

0 . 9 

0 . 7 

O.S 

ij/C MR 

1.0 

3 . 1 

5 . 3 

0 . 5 

0 . ° 

0 . 9 

1. 3 

1.1 

G/C MR 

1.3 

4 . 6 

5 . 9 

1.1 

1.1 

1.1 

1.1 

1.0 

I 

MEC 

; J 

'MEC 

MEC 
S:SOLVENT P:P0SIT1VE N:ANTHRAMINE W/0 S9 «-:TEST COMPOUNO 

CCN:i914a2 CHEM:EO STRAIN:97 DATE:042783 ACTIV: ACTX:C5 

P.96 

80G.0 

700.0 

600.? 

900.0 • 

; 

! 

; 

i 

• 

! 

• 

T 

; 

• 

i 

! 

; 

! 

• 

! 

i 

i 

; 

500.0 • 

f 

! 

; 

f 

42C.C • 

; 

j 

i 

300.3 • 

• 

! 

! 

; 

230.0 • 

! 

i 

PLOT OF AVGCOUNT*DOSE SYMBOL IS VALUE OF SPIND 

1CCC 

O.C 

1? ICC 

DOSE I N MICROGRAMS 

10 30 1 0 0 c 

187 
S:SOLVENT P:POSITIVE N:ANTHRAMINE W/C S9 *:TEST COMPOUNO 

C0N:1514£.2 CHEM:EO STRAIN:97 DATE:C42763 ACTIV:CSD0C2 ACTX:05 

PLCT CF AVGCOUNT»DOSE - SYMBOL IS VALUE GF SPIND 

R 

T 

A 

\ 

T 

3 

U 

N 

T 

500. C 

800.0 • 

700.C • 

600.0 

533.C • 

403.0 • 

333.0 • 

<: J -j . u • 

ic: 

o.o • 

10 13? 

DOSE IN MICROGRAMS 

13C0 IOC 30 

188 
S:SOLVENT P:POSITIVE N:ANTHRAMINE W/0 S9 »:TEST COMPOUND 

C0N:191432 CHEM:EO STRAIN:57 DATE=C42733 ACTIV:HEPA01 ACTi:05 

1 

PLCT CF AVGCOUNT*DOSE ' SYMBOL IS VALUE OF SPIND 

! 

900. 0 • 

! 

; 

! 

800. 0 • 

! 

700. 0 • 

600.C • 

5CC 0 • 

< i 

l ' 

; ' • 

4 0 0. 3 • 

f 

300. 0 • 

! 

; 

; 

• 

200. 0 • 

13C: 

! S 

! 

i 

• 

i 

• 

i 

i 

O.C • 

IC ICO 

DOSE IN MICROGRAMS 

10 0 0 10 0 0 0 

189 
P.99 

MUTAGENICITY CF EO - TA9t f l 

MUTAGENI C 

EXP DATE: 3427B 3 

TEST TYPE: 05 

DILUTION : : 

MIXTURE: 

T Y 

CHEMICAL : EO 

STRAIN : TA9 8 

PLATED : 

TECHNICIAN : LGM 

CONTRAC T NO. 

ACTIVATION : 

BATCH: 0263 

COLONIES: 

1914e2 

) 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE 

COUNT 

A : 

BG 

PLATE 

COUNT 

b: 

BG 

PLATE 

COUNT 

c: 

BG 

AVERAGE 

COUNT 

STD DEV 

COUNT 

w/C MR MEC 

SI 

P I 

P2 

55 

03 

03 

0.3C 

1.0 0 

2.0 0 

1.0 0 

3.CC 

11.3 0 

37.CC 

111.0 0 

44 

667 

186 

19 

17 

15 

32 

15 

29 

774 

149 

29 

13 

25 

21 

33 

33 

883 

175 

27 

23 

23 

17 

15 

34.3 

774.7 

170.C 

25.0 

17.7 

21.0 

23.3 

22.3 

8.4 

108.0 

19.0 

5.3 

5.0 

5.3 

7.B 

9.5 

1.0 

22.6 

5.C 

0.7 

0.5 

3.6 

0.7 

0.7 

M U T A G 

EXP DATE : 

TEST TYPE 

DILUTION: 

C N I C 

C42763 

: 05 

i: 20 

MIXTURE: 500 

I T Y 

CHEMICAL : EO 

STRAIN : TA96 

PLATED: IOC 

TECHNICIAN: LGM 

CONTRACT NO.: 191482 

ACTIVATION: CSD0C2 

BATCh: D263 

COLONIES: 

s 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

PLATE A: 

COUNT BG 

PLAT E e: 

COUNT BG 

PLATE C: 

COUNT BG 

AVERAGE 

COUNT 

STD OEV 

COUNT 

G/C MR MEC 

SI 

Fl 

P2 

55 

04 

04 

O.uC 

1.00 

2.0C 

1.0 3 

3.CC 

11.00 

37.00 

111.DC 

32 

429 

959 

3? 

33 

30 

43 

3 8 

32 

395 

1024 

4G 

39 

25 

24 

2S 

33 

387 

808 

31 

29 

33 

24 

34 

32.3 

405.0 

930.3 

33.7 

33.7 

31.0 

32.0 

33.3 

0.6 

21.6 

110.8 

5.5 

5.0 

6.6 

13.9 

5.3 

l.C 

12.5 

26.6 

1.0 

1.0 

1.0 

1.0 

1.0 

M U T A .G N I C I T Y 

IX.P DA-TE: ?/427e3 

TES T TYPE : 05 

DILUTION : 1.* 2 0 

MIXTURE : 5C 0 

CTnEMICAL.* EO 

STRAIN: TA98 

PLATED: 100 

TECHNICIAN: L -3 M 

CONTRAC T NO. : 19148 2 

ACTIVATION : HEPAC I 

bATCh: D263 

COLONIES: 

SOLVENT/ 

POSITIVE 

DOSE 

LEVEL 

FLATE A: 

CCUNT BO 

PLAT E •:: 

COUNT BG 

PLATE C: 

COUNT BG 

AVERAGE 

COUNT 

STD DEV 

COUNT 

u/C MR MEC 

SI 

Pi 

P2 

55 

04 

04 

O.UC 

0.50 

1.03 

l.C 3 

3.00 

11.00 

37.30 

111.03 

46 

711 

1094 

41 

37 

31 

32 

3 6 

16 

839 

1333 

38 

42 

28 

34 

36 

55 

754 

1362 

39 

30 

25 

30 

36 

35.C 

755. 3 

1159. 7 

39. 3 

36. 3 

2e.c 

3 2. 0 

36. 7 

2 C 4 

49. 1 

124. 3 

1 . 5 

6 . 0 

3 . 0 

2 . 0 

1.2 

l . C 

19. 4 

29. 7 

1.3 

0 . c 

3 . 7 

0 .5 

0 . 9 

190 

aate 
„ l - - I 

S = SOLVE M P = P0SITIV E N=ANTHRAMINE y/ C S9 *=TES T COMPOUND 

C0?J = 191<»82 CHEMrEO STRAIN=9 8 DATE=C4278 3 ACTIV = ACTX=C5 

P.1 

PLOT OF AV6C0UtvT*D0SE SYMBOL I S VALUE OF SPIN D 

E 

V 

T 

A 

N 

T 

0 

U 

N 

T 

70C. C 

SOO.C • 

i 

! 

! 

• 

800.0 • 

! 

! 

• 

! 

• 

• 

i 

I 

i 

• 

• 

! 

! 

! 

• 

! 

! 

; 

i 

400. 0 • 

so: 

300. 0 • 

; 

i 

! 

! 

20C.C • 

i 

! 

! 

i 

i o o . r< • 

! 

! 

! 

• 

0.0 • 

; t 

• 

1 

s 

10 ICC 

. . . 4 . . 

100 3 ICO CO 

TOSE I N KICROGF.AMS 

191 
P.2 

S = SOLV£NT P = PuSlTIv£ l '* = ANTHR AM INE y/ 0 S9 *=TEST COMPOUND 

CON=l°145 2 CHEMrEC STRAIN=9 6 DATE-C4278 3 ACTIV=CSDC0 2 ACTX=: 5 

1100. 0 • 

1000, 0 • 

QOO.O 

PLOT OF AVGCOU^T'DOSE SYMBOL I S VALUE OF SPIN D 

1

 «l 

8 0 0. 0 

700. 0 • 

bOC. 0 

5C0. 0 

-40C.C 

33C. 0 

20C. 0 

100. c 

o . c • 

io i: : 

COSE I N MICROGRAMS 

192 

100 0 

*a4»««B»«*w.a.M4>«.»..«>.aaaa4«»»*..»a..a«4/..».*.a.»«..4).. a . aal o».B«i a 

ICO wC 
P.3 

S=SOLVENT P=POSITlVE NSAMTHRAMINF. ./0 S9 *=TEST COMPOUND 

C0N=191482 CHEM=EO STRAIN=98 DATE=C42763 ACTIV=HEPA01 

» 

1500.0 • 

! 

! 

1400.0 • 

! 

i 

1300.0 • 

! 

! 

12C0.0 • 

• 

I 

1100.0 • 

! 

t 

1000.0 • 

I 

! 

900.0 • 

! 

! 

oCC.O • 

• 

! 

7CC.0 • 

; 

l 

6GC.0 • 

i 

; 

5CC.0 • 

! 

f 

400.0 • 

I 

! 

302 . 0 •» 

• 

j 

20G.0 • 

i 

i 

ice.a • 

! 

! S 

G.O • 

- —• . 

ACTX = 05' 

PLO T OF AVGCOU'JT.OOSE SYMBOL IS VALUE OF SPIN D 

10 i:c 

COSE IK MICROGRAM S 

1 3 Cu ' 1 P 0 C- C 

1 

1 

193 
MUTAGENICITY OF EO - TA10C 

P.4 

U T A G 

XP DATE: 

EST TYPE 

ILUTION= 

IxTURE= 

OLVENT/ 

OSITIVE 

SI 55 

PI 01 

P2 01 

E N I C I T Y 

042783 

: 05 

• • 

DOSE 

LEVEL 

0.0 0 

i.:c 

2.CC 

1.00 

3.CC 

11.00 

37.00 

111.00 

CHEMICAL : EO 

STRAIN 

PLATED: 

: TA100 

TECHNICIAN: LGM 

PLATE A: 

COUNT BG 

157 

565 

769 

234 

205 

252 

442 

551 

PLATE 

COUNT 

220 

549 

813 

£66 

223 

247 

380 

590 

B : 

E>G 

CONTRACT NO.: 

ACTIVATION : 

BATCH: D263 

COLOMES = 

PLATE 

COUNT 

110 

598 

674 

2*7 

239 

243 

432 

566 

c: 

BG 

AVERAGE 

COUNT 

162.3 

577.0 

752.0 

249.7 

222.3 

247.3 

416.0 

569.0 

191482 

STD DEV 

COUNT 

55.2 

25.9 

71.0 

17.2 

17.0 

4.5 

33 i 3 

19.7 

Q/C 

A 

A 

A 

MR 

1.0 

3.6 

4.6 

1.5 

1.4 

1.5 

2.6 

3.5 

MEC 

•r 

j! 

V= 

U T A G E H I C 

XF DATE: 042763 

EST TYPE : 05 

RUTIOu= i : 2 0 

IXTU?E= SCO 

T Y 

CHEMICAL : EO 

STRAIN : TA103 

PLATED= ICO 

TECHNICIAN : LGM 

CONTRACT NO.: 191482 

ACTIVATION CSO0C2 

BATCh: D263 

COLOMES = 

OLVENT/ 

OSITIVE 

SI 

PI 

P2 

55 

04 

04 

DOSE 

LEVEL 

C C C 

1.00 

2.:o 

l.CC 

3.c: 

n.:o 

37.00 

111.CO 

PLATE A: 

COUNT 3G 

152 

86 i. 

1603 

246 

182 

181 

2 17 

3C2 

U T A G E M I C I T Y 

PLATE 3 : 

CCUtJT RG 

220 

870 

17*9 

281 

2*8 

190 

227 

2 85 

XP DATE: C427B3 

EST TYPE: 05 

ILUTIO.N= i: 2 0 

IXTURE= 500 

CHEMICAL : EO 

STRAIN: TAIC: 

PLATED= 100 

TECHNICIAN: LGM 

PLATE 

COUNT 

369 

89? 

1679 

183 

ISa 

184 

197 

2C3 

c: 

Bt 

AVERAGE 

COUNT 

24 7. C 

884. 7 

1680. 3 

236. 7 

212. 7 

133. 0 

213. 7 

263. 3 

STD DEV 

COUNT 

111. 0 

14.C 

78. 0 

49. 7 

48. 0 

2 . 6 

15. 3 

52. 9 

Q/C MR M£C 

CONTRACT NO.: 1914P.2 

ACTIVATION: HEFAC1 

BATCH: D2s3 

COLOMES = 

NA 

A 

A 

1.0 

3.6 

6.S 

1.0 

C.9 

0.7 

0.9 

1.1 

.1 

1 

OLVENT/ 

OSITIVE 

DOSE 

LEVEL 

FLATE A: 

COUNT BG 

PLATE e: 

CGU.NT 9& 

PLATE C: 

COUNT BG 

AVERAGE 

COUNT 

S TD DEV 

COUNT 

G/C MR MEC 

SI 55 

FI 04 

F2 04 

o.:o 

0.50 

1.C0 

l.CC 

3.00 

11.CC 

37.00 

III.O : 

154 

1016 

186(> 

297 

284 

324 

254 

291 

156 

876 

17b5 

1S6 

237 

2°5 

269 

413 

167 

1101 

1773 

165 

196 

264 

3C0 

357 

155. 0 

997. 7 

1308. 0 

216. 0 

239. 0 

294. 3 

264. 3 

353. 7 

7 . 0 

113. 6 

6 7 . 7 

70. 5 

4 4 . 0 

30. 0 

15. 5 

61. 1 

1.0 

b.l 

11. 4 

1.4 

1.5 

1.9 

1.5 

2 . 2 

194 

1 l Jl. -WJOWJUllil.. I. J T -4 ,«ttt 

1 
P.5 

S=SOLVENT P=POSITIVE N=ANTHRAMINE b/C S9 «=TEST COMPOUNO 

C0N=1914b2 CHEM=EO STRAIN=190 DAJE=C42783 ACTIV= ACTX=0b 

! 

900.0 • 

; 

! 

! 

j 

800.0 • 

! 

! 

! 

; 

700.0 • 

! 

; 

• 

! 

600.C • 

500.G • 

t 

! 

i 

! 

• 

f 

! 

! 

• 

4CC.C 

300.0 • 

! 

200.C • 

1CC.C • 

; 

; 

! 

i 

0.0 • 

PLOT OF AVGCOU.NT*DOSE SYMBOL IS VALUE OF SP1NO 

IC ICO 

COSE IK KICROGP.AMS 

1 95 

10C 0 ICC CO 

ii 

I 

I 

i ?i 
P.6 

C0N=19 

t o , or mcou<,.Bo«E srB6 oL „ VALUE or SP. N 0 

1600.0 « 

1600.0 • 

1400.0 • 

120C.C • 

; 

1CCC.C • 

POC.O • 

i 

6 0 0.0 • 

40C.C • 

i 

! S 

2 C 0 . C • 

; 

0.0 • 

ACTXS05 

1 

•«ca 

10 i:o 10C0 

DOSE IN MICROGRAMS 

ico;o 

196 
P.7 

S=S0LVENT P=PCSITIVE U=ANTHR*MINE W/0 S9 *=TEST COMPOUNG 

C0N = 191462 CHEM = EO STRAIN=KC DATE»C«.27fe3 ACTIV=HEPA01 ACT*=05 

1800. 0 • 

1600. 0 • 

14C0.0 • 

120C 0 • 

1000. 0 

6C0.0 

600. 0 

40C.0 

20C.C 

0 . 0 

PLOT OF AVGCOUNT-OOSC SrM"OL IS VALUC OF SPI\ D 

10 IOC 

DOSE IN MICROGRAMS 

ICC J 

, 

{ 

1CC.C 

r7 
i^SSS 

P.8