### 13339 Hagers Ferry Road Huntersville, NC 28078-7929 McGuire Nuclear Complex - MG03A2 Phone: 980-875-5245 Fax: 980-875-4349 

# Order Summary Report 

# Analytical Laboratory

Order Number: J12090176 Project Name: Somerville Waste Water

Lab Contact: Jason C Perkins 

### Date: 10/5/2012 Customer Address: 253 Plant Allen Road Customer Name(s): BillK-RonL--RobnJ-DonS

Belmont, NC 28012

Phone: 980-875-5348 Report Authorized By: (Signature)


### Program Comments: Please contact the Program Manager (Jason C Perkins) with any questions regarding this report. Data Flags & Calculations: Any analytical tests or individual analytes within a test flagged with a Qualifier indicate a deviation from the method quality system or quality control requirement. The qualifier description is found at the end of the Certificate of Analysis (sample results) under the qualifiers heading. All results are reported on a dry weight basis unless otherwise noted. Subcontracted data included on the Duke Certificate of Analysis is to be used as information only. Certified vendor results can be found in the subcontracted lab final report. Duke Energy Analytical Laboratory subcontracts analyses to other vendor laboratories that have been qualified by Duke Energy to perform these analyses except where noted. Data Package: This data package includes analytical results that are applicable only to the samples described in this narrative. An estimation of the uncertainty of measurement for the results in the report is available upon request. This report shall not be reproduced, except in full, without the written consent of the Analytical Laboratory. Please contact the Analytical laboratory with any questions. The order of individual sections within this report is as follows: Job Summary Report, Sample Identification, Technical Validation of Data Package, Analytical Laboratory Certificate of Analysis, Analytical Laboratory QC Reports, Sub-contracted Laboratory Results, Customer Specific Data Sheets, Reports & Documentation, Customer Database Entries, Test Case Narratives, Chain of Custody (COC) Certification: The Analytical Laboratory holds the following State Certifications : North Carolina (DENR) Certificate #248, South Carolina (DHEC) Laboratory ID # 99005. Contact the Analytical Laboratory for definitive information about the certification status of specific methods.

## Sample ID's & Descriptions:

Sample ID Plant/Station Collection Date and Time Collected By Sample Description 2012019722 ALLEN 11-Sep-12 3:30 AM Chris Greene FGD Purge Eff 2012019723 ALLEN 11-Sep-12 8:19 AM BILL HASKINS EQ Tank Eff 2012019724 ALLEN 11-Sep-12 8:15 AM BILL HASKINS BioReactor 1 Inf 2012019725 ALLEN 11-Sep-12 8:40 AM BILL HASKINS BioReactor 1 Inf BLANK 2012019726 ALLEN 11-Sep-12 8:27 AM BILL HASKINS BioReactor 2 Inf 2012019727 ALLEN 11-Sep-12 8:48 AM BILL HASKINS BioReactor 2 Inf BLANK 2012019728 ALLEN 11-Sep-12 8:23 AM BILL HASKINS BioReactor 2 Eff 2012019729 ALLEN 11-Sep-12 8:44 AM BILL HASKINS BioReactor 2 Eff BLANK 2012019730 ALLEN 11-Sep-12 10:40 AM BILL HASKINS Filter Blk 9 Total Samples

### COC and .pdf report are in agreement with sample totals

and analyses (compliance programs and procedures). All Results are less than the laboratory reporting limits. All laboratory QA/QC requirements are acceptable. Yes No



# Technical Validation Review Checklist:

Yes No

Yes No



## Report Sections Included:

Job Summary Report Sub-contracted Laboratory Results Sample Identification Customer Specific Data Sheets, Reports, & Documentation Technical Validation of Data Package Customer Database Entries Analytical Laboratory Certificate of Analysis Analytical Laboratory QC Report Chain of Custody

Reviewed By: 

### DataBase Administrator Date: 

### 10/5/2012 Electronic Data Deliverable (EDD) Sent Separately

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12090176

2012019722 Collection Date: 11-Sep-12 3:30 AM 

### Site: FGD Purge Eff Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF ALKALINITY - (Analysis Performed by Prism Labs) Vendor Parameter Complete Vendor Method V_PRISM
INORGANIC IONS BY IC Bromide 1200 mg/L EPA 300.0 9/17/2012 5:09:00 PM JAHERMA 50 500 Chloride 2500 mg/L EPA 300.0 9/17/2012 5:09:00 PM JAHERMA 50 500 Sulfate 1300 mg/L EPA 300.0 9/17/2012 5:09:00 PM JAHERMA 50 500 MERCURY (COLD VAPOR) IN WATER Mercury (Hg) 25.8 ug/L EPA 245.1 9/20/2012 1:37:34 PM AGIBBS 2.5 50 DISSOLVED METALS BY ICP Manganese (Mn) 1.73 mg/L EPA 200.7 9/12/2012 2:09:00 PM MHH7131 0.05 10 TOTAL RECOVERABLE METALS BY ICP Boron (B) 78.9 mg/L EPA 200.7 9/26/2012 12:38:00 PM DJSULL1 0.5 10 Calcium (Ca) 4050 mg/L EPA 200.7 9/26/2012 12:38:00 PM DJSULL1 0.1 10 Iron (Fe) 123 mg/L EPA 200.7 9/26/2012 12:38:00 PM DJSULL1 0.1 10 Lithium (Li) 0.220 mg/L EPA 200.7 9/26/2012 12:38:00 PM DJSULL1 0.05 10 Magnesium (Mg) 586 mg/L EPA 200.7 9/26/2012 12:38:00 PM DJSULL1 0.05 10 Manganese (Mn) 4.70 mg/L EPA 200.7 9/26/2012 12:38:00 PM DJSULL1 0.05 10 Potassium (K) 46.9 mg/L EPA 200.7 9/26/2012 12:38:00 PM DJSULL1 1 10 Sodium (Na) 30.2 mg/L EPA 200.7 9/26/2012 12:38:00 PM DJSULL1 0.5 10 DISSOLVED METALS BY ICP-MS Selenium (Se) 4740 ug/L EPA 200.8 9/18/2012 4:00:00 PM KRICHAR 10 10 TOTAL RECOVERABLE METALS BY ICP-MS Arsenic (As) 278 ug/L EPA 200.8 9/25/2012 12:19:00 PM KRICHAR 10 10 Cadmium (Cd) < 10 ug/L EPA 200.8 9/25/2012 12:19:00 PM KRICHAR 10 10 Chromium (Cr) 216 ug/L EPA 200.8 9/25/2012 12:19:00 PM KRICHAR 10 10 Copper (Cu) 202 ug/L EPA 200.8 9/25/2012 12:19:00 PM KRICHAR 10 10 Nickel (Ni) 278 ug/L EPA 200.8 9/25/2012 12:19:00 PM KRICHAR 10 10 Selenium (Se) 7060 ug/L EPA 200.8 9/25/2012 12:19:00 PM KRICHAR 20 20 Silver (Ag) < 10 ug/L EPA 200.8 9/25/2012 12:19:00 PM KRICHAR 10 10 Zinc (Zn) 354 ug/L EPA 200.8 9/25/2012 12:19:00 PM KRICHAR 10 10 SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC) Vendor Parameter Complete Vendor Method V_AS&C
TOTAL DISSOLVED SOLIDS TDS 14000 mg/L SM2540C 9/12/2012 3:51:00 PM SWILLI3 200 1 TOTAL SUSPENDED SOLIDS TSS 2800 mg/L SM2540D 9/13/2012 1:55:00 PM SWILLI3 250 1

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12090176

2012019722 Collection Date: 11-Sep-12 3:30 AM 

### Site: FGD Purge Eff Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF


### 2012019723 Collection Date: 11-Sep-12 8:19 AM 

### Site: EQ Tank Eff Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF MERCURY (COLD VAPOR) IN WATER Mercury (Hg) 19.9 ug/L EPA 245.1 9/20/2012 1:39:58 PM AGIBBS 2.5 50 DISSOLVED METALS BY ICP Manganese (Mn) 0.370 mg/L EPA 200.7 9/12/2012 2:12:00 PM MHH7131 0.05 10 TOTAL RECOVERABLE METALS BY ICP Boron (B) 88.4 mg/L EPA 200.7 9/26/2012 12:42:00 PM DJSULL1 0.5 10 Calcium (Ca) 2050 mg/L EPA 200.7 9/26/2012 12:42:00 PM DJSULL1 0.1 10 Iron (Fe) 69.1 mg/L EPA 200.7 9/26/2012 12:42:00 PM DJSULL1 0.1 10 Lithium (Li) 0.153 mg/L EPA 200.7 9/26/2012 12:42:00 PM DJSULL1 0.05 10 Magnesium (Mg) 544 mg/L EPA 200.7 9/26/2012 12:42:00 PM DJSULL1 0.05 10 Manganese (Mn) 2.81 mg/L EPA 200.7 9/26/2012 12:42:00 PM DJSULL1 0.05 10 Potassium (K) 31.3 mg/L EPA 200.7 9/26/2012 12:42:00 PM DJSULL1 1 10 Sodium (Na) 34.8 mg/L EPA 200.7 9/26/2012 12:42:00 PM DJSULL1 0.5 10 DISSOLVED METALS BY ICP-MS Selenium (Se) 3780 ug/L EPA 200.8 9/18/2012 3:44:00 PM KRICHAR 10 10 TOTAL RECOVERABLE METALS BY ICP-MS Arsenic (As) 140 ug/L EPA 200.8 9/25/2012 12:22:00 PM KRICHAR 10 10 Cadmium (Cd) < 10 ug/L EPA 200.8 9/25/2012 12:22:00 PM KRICHAR 10 10 Chromium (Cr) 133 ug/L EPA 200.8 9/25/2012 12:22:00 PM KRICHAR 10 10 Copper (Cu) 127 ug/L EPA 200.8 9/25/2012 12:22:00 PM KRICHAR 10 10 Nickel (Ni) 198 ug/L EPA 200.8 9/25/2012 12:22:00 PM KRICHAR 10 10 Selenium (Se) 4720 ug/L EPA 200.8 9/25/2012 12:22:00 PM KRICHAR 10 10 Silver (Ag) < 10 ug/L EPA 200.8 9/25/2012 12:22:00 PM KRICHAR 10 10 Zinc (Zn) 239 ug/L EPA 200.8 9/25/2012 12:22:00 PM KRICHAR 10 10


### 2012019724 Collection Date: 11-Sep-12 8:15 AM 

### Site: BioReactor 1 Inf Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF ALKALINITY - (Analysis Performed by Prism Labs) Vendor Parameter Complete Vendor Method V_PRISM

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12090176

2012019724 Collection Date: 11-Sep-12 8:15 AM 

### Site: BioReactor 1 Inf Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND
DISSOLVED METALS BY ICP Manganese (Mn) < 0.05 mg/L EPA 200.7 9/12/2012 2:15:00 PM MHH7131 0.05 10 TOTAL RECOVERABLE METALS BY ICP Boron (B) 89.4 mg/L EPA 200.7 9/26/2012 12:46:00 PM DJSULL1 0.5 10 Calcium (Ca) 1640 mg/L EPA 200.7 9/26/2012 12:46:00 PM DJSULL1 0.1 10 Iron (Fe) < 0.1 mg/L EPA 200.7 9/26/2012 12:46:00 PM DJSULL1 0.1 10 Lithium (Li) 0.066 mg/L EPA 200.7 9/26/2012 12:46:00 PM DJSULL1 0.05 10 Magnesium (Mg) 348 mg/L EPA 200.7 9/26/2012 12:46:00 PM DJSULL1 0.05 10 Manganese (Mn) < 0.05 mg/L EPA 200.7 9/26/2012 12:46:00 PM DJSULL1 0.05 10 Potassium (K) 16.6 mg/L EPA 200.7 9/26/2012 12:46:00 PM DJSULL1 1 10 Sodium (Na) 64.6 mg/L EPA 200.7 9/26/2012 12:46:00 PM DJSULL1 0.5 10 DISSOLVED METALS BY ICP-MS Selenium (Se) 2470 ug/L EPA 200.8 9/18/2012 3:47:00 PM KRICHAR 10 10 TOTAL RECOVERABLE METALS BY ICP-MS Arsenic (As) < 10 ug/L EPA 200.8 9/25/2012 12:25:00 PM KRICHAR 10 10 Cadmium (Cd) < 10 ug/L EPA 200.8 9/25/2012 12:25:00 PM KRICHAR 10 10 Chromium (Cr) < 10 ug/L EPA 200.8 9/25/2012 12:25:00 PM KRICHAR 10 10 Copper (Cu) < 10 ug/L EPA 200.8 9/25/2012 12:25:00 PM KRICHAR 10 10 Nickel (Ni) < 10 ug/L EPA 200.8 9/25/2012 12:25:00 PM KRICHAR 10 10 Selenium (Se) 2730 ug/L EPA 200.8 9/25/2012 12:25:00 PM KRICHAR 10 10 Silver (Ag) < 10 ug/L EPA 200.8 9/25/2012 12:25:00 PM KRICHAR 10 10 Zinc (Zn) < 10 ug/L EPA 200.8 9/25/2012 12:25:00 PM KRICHAR 10 10 SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC) Vendor Parameter Complete Vendor Method V_AS&C


### 2012019725 Collection Date: 11-Sep-12 8:40 AM 

### Site: BioReactor 1 Inf BLANK Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12090176

2012019725 Collection Date: 11-Sep-12 8:40 AM 

### Site: BioReactor 1 Inf BLANK Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND


### 2012019726 Collection Date: 11-Sep-12 8:27 AM 

### Site: BioReactor 2 Inf Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF ALKALINITY - (Analysis Performed by Prism Labs) Vendor Parameter Complete Vendor Method V_PRISM MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND DISSOLVED METALS BY ICP Manganese (Mn) < 0.05 mg/L EPA 200.7 9/12/2012 2:18:00 PM MHH7131 0.05 10 TOTAL RECOVERABLE METALS BY ICP Boron (B) 94.7 mg/L EPA 200.7 9/26/2012 12:50:00 PM DJSULL1 0.5 10 Calcium (Ca) 1640 mg/L EPA 200.7 9/26/2012 12:50:00 PM DJSULL1 0.1 10 Iron (Fe) 0.247 mg/L EPA 200.7 9/26/2012 12:50:00 PM DJSULL1 0.1 10 Lithium (Li) 0.069 mg/L EPA 200.7 9/26/2012 12:50:00 PM DJSULL1 0.05 10 Magnesium (Mg) 355 mg/L EPA 200.7 9/26/2012 12:50:00 PM DJSULL1 0.05 10 Manganese (Mn) < 0.05 mg/L EPA 200.7 9/26/2012 12:50:00 PM DJSULL1 0.05 10 Potassium (K) 28.1 mg/L EPA 200.7 9/26/2012 12:50:00 PM DJSULL1 1 10 Sodium (Na) 64.9 mg/L EPA 200.7 9/26/2012 12:50:00 PM DJSULL1 0.5 10 DISSOLVED METALS BY ICP-MS Selenium (Se) 164 ug/L EPA 200.8 9/18/2012 3:50:00 PM KRICHAR 10 10 TOTAL RECOVERABLE METALS BY ICP-MS Arsenic (As) < 10 ug/L EPA 200.8 9/25/2012 12:28:00 PM KRICHAR 10 10 Cadmium (Cd) < 10 ug/L EPA 200.8 9/25/2012 12:28:00 PM KRICHAR 10 10 Chromium (Cr) < 10 ug/L EPA 200.8 9/25/2012 12:28:00 PM KRICHAR 10 10 Copper (Cu) < 10 ug/L EPA 200.8 9/25/2012 12:28:00 PM KRICHAR 10 10 Nickel (Ni) < 10 ug/L EPA 200.8 9/25/2012 12:28:00 PM KRICHAR 10 10 Selenium (Se) 185 ug/L EPA 200.8 9/25/2012 12:28:00 PM KRICHAR 10 10 Silver (Ag) < 10 ug/L EPA 200.8 9/25/2012 12:28:00 PM KRICHAR 10 10 Zinc (Zn) < 10 ug/L EPA 200.8 9/25/2012 12:28:00 PM KRICHAR 10 10

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12090176

2012019726 Collection Date: 11-Sep-12 8:27 AM 

### Site: BioReactor 2 Inf Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC) Vendor Parameter Complete Vendor Method V_AS&C



### 2012019727 Collection Date: 11-Sep-12 8:48 AM 

### Site: BioReactor 2 Inf BLANK Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND



### 2012019728 Collection Date: 11-Sep-12 8:23 AM 

### Site: BioReactor 2 Eff Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF ALKALINITY - (Analysis Performed by Prism Labs) Vendor Parameter Complete Vendor Method V_PRISM
INORGANIC IONS BY IC Bromide 520 mg/L EPA 300.0 9/17/2012 5:27:00 PM JAHERMA 50 500 Chloride 2100 mg/L EPA 300.0 9/17/2012 5:27:00 PM JAHERMA 50 500 Sulfate 1500 mg/L EPA 300.0 9/17/2012 5:27:00 PM JAHERMA 50 500 MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND
DISSOLVED METALS BY ICP Manganese (Mn) < 0.05 mg/L EPA 200.7 9/12/2012 2:21:00 PM MHH7131 0.05 10

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12090176

2012019728 Collection Date: 11-Sep-12 8:23 AM 

### Site: BioReactor 2 Eff Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF TOTAL RECOVERABLE METALS BY ICP Boron (B) 113 mg/L EPA 200.7 9/26/2012 12:54:00 PM DJSULL1 0.5 10 Calcium (Ca) 1490 mg/L EPA 200.7 9/26/2012 12:54:00 PM DJSULL1 0.1 10 Iron (Fe) 1.22 mg/L EPA 200.7 9/26/2012 12:54:00 PM DJSULL1 0.1 10 Lithium (Li) 0.063 mg/L EPA 200.7 9/26/2012 12:54:00 PM DJSULL1 0.05 10 Magnesium (Mg) 391 mg/L EPA 200.7 9/26/2012 12:54:00 PM DJSULL1 0.05 10 Manganese (Mn) < 0.05 mg/L EPA 200.7 9/26/2012 12:54:00 PM DJSULL1 0.05 10 Potassium (K) 30.3 mg/L EPA 200.7 9/26/2012 12:54:00 PM DJSULL1 1 10 Sodium (Na) 65.3 mg/L EPA 200.7 9/26/2012 12:54:00 PM DJSULL1 0.5 10 DISSOLVED METALS BY ICP-MS Selenium (Se) 15.2 ug/L EPA 200.8 9/18/2012 3:53:00 PM KRICHAR 5 5 TOTAL RECOVERABLE METALS BY ICP-MS Arsenic (As) < 5 ug/L EPA 200.8 9/25/2012 12:31:00 PM KRICHAR 5 5 Cadmium (Cd) < 5 ug/L EPA 200.8 9/25/2012 12:31:00 PM KRICHAR 5 5 Chromium (Cr) < 5 ug/L EPA 200.8 9/25/2012 12:31:00 PM KRICHAR 5 5 Copper (Cu) < 5 ug/L EPA 200.8 9/25/2012 12:31:00 PM KRICHAR 5 5 Nickel (Ni) < 5 ug/L EPA 200.8 9/25/2012 12:31:00 PM KRICHAR 5 5 Selenium (Se) 16.0 ug/L EPA 200.8 9/25/2012 12:31:00 PM KRICHAR 5 5 Silver (Ag) < 5 ug/L EPA 200.8 9/25/2012 12:31:00 PM KRICHAR 5 5 Zinc (Zn) < 5 ug/L EPA 200.8 9/25/2012 12:31:00 PM KRICHAR 5 5 SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC) Vendor Parameter Complete Vendor Method V_AS&C



### 2012019729 Collection Date: 11-Sep-12 8:44 AM 

### Site: BioReactor 2 Eff BLANK Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC) Vendor Parameter Complete Vendor Method V_BRAND

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12090176

2012019730 Collection Date: 11-Sep-12 10:40 AM 

### Site: Filter Blk Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF DISSOLVED METALS BY ICP Manganese (Mn) < 0.005 mg/L EPA 200.7 9/12/2012 2:24:00 PM MHH7131 0.005 1 DISSOLVED METALS BY ICP-MS Selenium (Se) < 1 ug/L EPA 200.8 9/18/2012 2:26:00 PM KRICHAR 1 1

## September 25 , 201 2

Jay Perkins Duke Energy Analytical Laboratory Mail Code MGO3A2 (Building 7405) 13339 Hagers Ferry Rd. Huntersville, NC 28078 (704) 875-5245 Project: Allen Shay/MillerCreek (LIMS#J1209017 6)

Dear Mr. Perkins, Attached is the report associated with four (4) aqueous samples submitted for selenium speciation analysis on September 13 , 2012

.The samples were received in a sealed cooler at -0. 5 °C on September 1 4, 2012

.Selenium speciation analysis was performed via ionchromatography inductively coupled plasma collision reaction cell mass spectrometry (IC-ICPCRC-MS). Any issues associated with the analysis are addressed in the following report. If you have any questions, please feel free to contact me at your convenience.
Sincerely,

Russell Gerads Vice President Applied Speciation and Consulting, LLC

## Applied Speciation and Consulting, LLC Report prepared for: Jay Perkins Duke Energy Analytical Laboratory Mail Code MGO3A2 (Building 7405) 13339 Hagers Ferry Rd. Huntersville, NC 28078 Project: Allen Shay/MillerCreek (LIMS#J12090176)

September 25 , 2012

1. Sample Reception Four (4) aqueous samples in125mL HDPE bottles (provided by Applied Speciation and Consulting) were submitted for selenium speciation analysis on September 1 3 , 2012 . The samples were received on September 1 4, 201 2 in a sealed container at -0. 5°C. The samples were received in a laminar flow clean hood,void of trace metals contamination and ultra-violet radiation, and were designated discrete sample identifiers. An aliquot of each sample was filtered (0.45μm) and each filtrate wasstored in a secure, monitored cryofreezer (maintained at a temperature of -80o

## C) until selenium speciation analysis could be performed via ion chromatography inductively coupled plasma collision reaction cell mass spectrometry (IC-ICP-CRC-MS).

2. Sample Preparation All sample preparation is performed in laminar flow clean hoods known to be free from trace metals contamination. All applied water for dilutions and sample preservatives are monitored for contamination to account for any biases associated with the sample results. Selenium Speciation Analysis by IC-ICP-CRC-MS Prior to analysis, an aliquot of each sample was filtered with a syringe filter (0.45μm) and injected directly into a sealed autosampler vial. No further sample preparation was performed as any chemical alteration of a sample may shift the equilibrium of the system, resulting in changes in speciation ratios. 3. Sample Analysis All sample analysis is preceded by a minimum of a five-point calibration curve spanning the entire concentration range of interest. Calibration curves are performed at the beginning of each analytical day. All calibration curves, associated with each species of interest, are

## standardized by linear regression resulting in a response factor. All sample results are

instrument blank correctedto account for any operational biases associated with the analytical platform. Prior to sample analysis, all calibration curves are verified using second source standards which are identified as initial calibration verification standards (ICV). Ongoing instrument performance is identified by the analysis of continuing calibration verification standards (CCV) and continuing calibration blanks (CCB) at a minimal interval of every ten analytical runs. Selenium Speciation Analysis by IC-ICP-CRC-MS Eachsample for selenium speciation analysis wasanalyzed by ion chromatography inductively coupled plasma collision reaction cell mass spectrometry (IC-ICP-CRC-MS) on September 21, 2012. An aliquot of each sample isinjected onto an anion exchange column and mobilized by a basic (pH > 7) gradient. The eluting selenium species are then introduced into a radio frequency (RF) plasma where energy-transfer processes cause desolvation, atomization, and ionization. The ions are extracted from the plasma through a differentially-pumped vacuum interface and travel through a pressurized chamber (CRC) containing a reaction gas which preferentially reacts with interfering ions of the same target mass to charge ratios (m/z). A solid-state detector detects ions transmitted through the mass analyzer and the resulting current is processed by a data handling system. Retention times for each eluting species are compared to known standards for species identification.

4. Analytical Issues The overall analyses went well and no significant analytical issues were encountered. All quality control parameters associated with the samples were within acceptance limits. The estimated method detection limits (eMDLs) for selenite, selenate, and selenocyanate are generated from replicate analyses of the lowest standard in the calibration curve. Not all selenium species are present in preparation blanks; therefore, eMDL calculations based on preparation blanks are artificially biased low. The eMDL for methylseleninic acid and selenomethionine is calculated from the average eMDL of selenite, selenate, and selenocyanate. The calibration does not contain methylseleninic acid or selenomethionine due to impurities in these standards which would bias the results for other selenium species.

## If you have any questions or concerns regarding this report, please feel free to contact me.

Sincerely,

Russell Gerads Vice President Applied Speciation and Consulting, LLC

### Sample Results

Sample ID Se(IV) Se(VI) SeCN MeSe(IV) SeMe Unknown Se Species (n) FGD Purge Eff 547 3840 ND (<1.2) ND (<0.92) ND (<0.92) 0.0 (0) BioReactor 1 Inf 8.09 2290 ND (<0.31) 1.30 0.24 0.0 (0) BioReactor 2 Inf 34.5 75.6 0.48 1.38 0.47 0.0 (0) BioReactor 2 Eff 0.99 ND (<0.12) ND (<0.31) ND (<0.23) ND (<0.23) 0.0 (0) All results reflect the applied dilution and are reported in μg/L ND = Not detected at the applied dilution SeCN = Selenocyanate MeSe(IV) = Methylseleninic acid SeMe = Selenomethionine Unknown Se Species = Total concentration of all unknown Se species observed by IC-ICP-MS n = number of unknown Se species observed Report Generated by: Russell Gerads Applied Speciation and Consulting, LLC Selenium Speciation Results for Duke Energy Project Name: Allen Shay/MillerCreek Contact: Jay Perkins
Date: September 25, 2012 LIMS #J12090176

### Quality Control Summary - Preparation Blank Summary

Analyte (μg/L) PBW1 PBW2 PBW3 PBW4 Mean StdDev eMDL* eMDL 250x eMDL 1000x Se(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.0010 0.26 1.0 Se(VI) 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0005 0.12 0.47 SeCN 0.000 0.000 0.000 0.000 0.000 0.000 0.0012 0.31 1.2 MeSe(IV) 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0009 0.23 0.92 SeMe 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0009 0.23 0.92 eMDL = Estimated Method Detection Limit *Please see narrative regarding eMDL calculations
Quality Control Summary - Certified Reference Materials Analyte (μg/L) CRM True Value Result Recovery Se(IV) LCS 9.57 9.30 97.2 Se(VI) LCS 9.48 8.94 94.3 SeCN LCS 8.92 8.60 96.4 MeSe(IV) LCS 6.47 6.07 93.9 SeMe LCS 9.32 8.67 93.1 Applied Speciation and Consulting, LLC Selenium Speciation Results for Duke Energy Project Name: Allen Shay/MillerCreek Contact: Jay Perkins LIMS #J12090176 Date: September 25, 2012 Report Generated by: Russell Gerads

### Quality Control Summary - Matrix Duplicates

Analyte (μg/L) Sample ID Rep 1 Rep 2 Mean RPD Se(IV) BioReactor 1 Inf 8.09 8.45 8.27 4.4 Se(VI) BioReactor 1 Inf 2290 2310 2300 0.9 SeCN BioReactor 1 Inf ND (<0.31) ND (<0.31) NC NC MeSe(IV) BioReactor 1 Inf 1.30 1.21 1.26 7.1 SeMe BioReactor 1 Inf 0.24 ND (<0.23) NC NC ND = Not detected at the applied dilution NC = Value was not calculated due to one or more concentrations below the eMDL

Quality Control Summary - Matrix Spike/ Matrix Spike Duplicate Analyte (μg/L) Sample ID Spike Conc MS Result Recovery Spike Conc MSD Result Recovery RPD Se(IV) BioReactor 1 Inf 1390 1365 97.6 1390 1361 97.3 0.3 Se(VI) BioReactor 1 Inf 1261 3484 93.9 1261 3504 95.4 0.6 SeCN BioReactor 1 Inf 1144 1076 94.1 1144 1060 92.7 1.5 Applied Speciation and Consulting, LLC Selenium Speciation Results for Duke Energy Project Name: Allen Shay/MillerCreek Contact: Jay Perkins LIMS #J12090176 Date: September 25, 2012 Report Generated by: Russell Gerads

### 3958 6th

### Ave NW • Seattle, WA 98107 • T: 206-632-6206 • F: 206-632-6017 • www.brooksrand.com • brl@brooksrand.com 

## October 1, 2012
Duke Energy ATTN: Jay Perkins Scientific Support-Laboratory 13339 Hagers Ferry Road Huntersville NC 28078 jcperkins@duke-energy.com labcustomer@duke-energy.com
RE: Project DUK-HV1201 Client Project: J12090176
Dear Mr. Perkins, On September 14, 2012, Brooks Rand Labs (BRL) received three (3) wastewater samples and three (3) corresponding field blanks. An aliquot was removed from each sample bottle and filtered into a separate container designed for dissolved mercury (Hg) analysis. The sample volume from the original container was logged-in for total Hg analysis. All samples were received, prepared, analyzed, and stored acco rding to BRL SOPs and EPA methodology.Data used for regulatory purposes has a 24 hour filtration holding time requirement. Nonregulatory purposed data has a 48 hour filtration holding time. The samples were received outside of the non-regulatory requirement holding time and were qualified H

. The results were blank-corrected as described in the calculations section of the relevant SOP and may have been evaluated using reporting limits that have been adjusted to account for sample aliquot size. Please refer to the Sample Results page for sample-specific MDLs, MRLs, and other details. In sequences 1200724 and 1200736, the results of continuing calibration blank –CCB1 were greater than the low calibration standard; however, no client samples were bracketed by the analysis of –CCB1 and all other CCBs results were low. The somewhat elevated –CCB1s were likely attributed to carryover from the previous analysis of the independent calibration verification standard -ICV1. In sequence 1200740, CCBB was greater than the low calibration standard. No samples from this work order were bracketed by the elevated CCB. All samples that were bracketed by this CCB were greater than 10x the concentration and no further action was required. The continuing calibration verification standard –CCV4, analyzed in sequence 1200724, recovered at 123%- above the acceptance criteria range. No client samples from this work order were bracketed. Aside from concentration qualifiers, all data was reported without further qualification and all associated quality control sample results met the acceptance criteria. BRL, an accredited laboratory, certifies the reported results of all analyses for which BRL is NELAP accredited meet all NELAP requirements. For more details, see the Report Information

## page of the report. Please feel free to contact me if you have any questions regarding this report. Sincerely,

Lydia Greaves Project Manager lydia@brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



### Definition of Data Qualifiers

(Effective 9/23/09) 

### Laboratory Accreditation BRL is accredited by the National Environmental Laboratory Accreditation Program (NELAP) through the State of Florida Department of Health, Bureau of Laboratories (E87982) and is certified to perform many environmental analyses. BRL is also certified by many other states to perform environmental analyses. For a current list of our accreditations /certifications, please visit our website at <http://www.brooksrand.com/default.asp?contentID=586>. Results reported relate only to the samples listed in the report. 

# Report Information

BLK BRL BS CAL CCV CRM D DUP ICV MSD ND NR PS REC RPD RSD SCV SOP 

### method blank Brooks Rand Labs laboratory fortified blank calibration standard continuing calibration verification certified reference material dissolved fraction duplicate initial calibration verification matrix spike duplicate non-detect non-reportable post preparation spike percent recovery relative percent difference relative standard deviation secondary calibration verification standard operating procedure MDL MRL MS



### method detection limit method reporting limit matrix spike

SRM T COC



### standard reference material total recoverable fraction chain of custody record 

### Common Abbreviations

These qualifiers are based on those previously utilized by Brooks Rand Labs, those found in the EPA SOW ILM03.0, Exhibit B, Section III, pg. B-18, and the USEPA Contract Laboratory Program National Functional Guidelines for Inorganic Superfund Data Review; USEPA; January 2010. These supersede all previous qualifiers ever employed by BRL. Detected by the instrument, the result is > the MDL but ≤the MRL. Result is reported and considered an estimate. B E 

### An estimated value due to the presence of interferences. A full explanation is presented in the narrative. H 

### Holding time and/or preservation requirements not met. Result is estimated. J 

### Estimated value. A full explanation is presented in the narrative. J-M 

### Duplicate precision (RPD) for associated QC sample was not within acceptance criteria. Result is estimated. J-N 

### Spike recovery for associated QC sample was not within acceptance criteria. Result is estimated. M 

### Duplicate precision (RPD) was not within acceptance criteria. Result is estimated. N 

### Spike recovery was not within acceptance criteria. Result is estimated. R 

### Rejected, unusable value. A full explanation is presented in the narrative. U 

### Result is ≤the MDL or client requested reporting limit (CRRL). Result reported as the MDL or CRRL. X 

### Result is not BLK-corrected and is within 10x the absolute value of the highest detectable BLK in the batch. Result is estimated. 

### Field Quality Control Samples Please be notified that certain EPA methods require the collection of field quality control samples of an appropriate type and frequency; failure to do so is considered a deviation from some methods and for compliance purposes should only be done with the approval of regulatory authorities. Please see the specific EPA methods for details regarding required field quality control samples.

3958 6th Avenue NW Seattle WA 98107 ·P(206) 632-6206 ·F(206) 632-6017 ·brl@brooksrand.com ·www.brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Sample Information

Report Matrix Type Received Sampled Sample Lab ID 

### 1237037-01 BioReactor 1 Inf 09/11/2012 09/14/2012 Sample Influent 1237037-02 BioReactor 1 Inf 09/11/2012 09/14/2012 Sample Influent 1237037-03 BioReactor 1 Inf Hg Blk 09/11/2012 09/14/2012 Field Blank DIW 1237037-04 BioReactor 1 Inf Hg Blk 09/11/2012 09/14/2012 Field Blank DIW 1237037-05 BioReactor 2 Inf 09/11/2012 09/14/2012 Sample Influent 1237037-06 BioReactor 2 Inf 09/11/2012 09/14/2012 Sample Influent 1237037-07 BioReactor 2 Inf Hg Blk 09/11/2012 09/14/2012 Field Blank DIW 1237037-08 BioReactor 2 Inf Hg Blk 09/11/2012 09/14/2012 Field Blank DIW 1237037-09 BioReactor 2 Eff 09/11/2012 09/14/2012 Sample Effluent 1237037-10 BioReactor 2 Eff 09/11/2012 09/14/2012 Sample Effluent 1237037-11 BioReactor 2 Eff Hg Blk 09/11/2012 09/14/2012 Field Blank DIW 1237037-12 BioReactor 2 Eff Hg Blk 09/11/2012 09/14/2012 Field Blank DIW 

# Batch Summary

Analyte Prepared Analyzed Sequence Batch Lab Matrix Method 

### B121708 09/18/2012 09/19/2012 1200724 Water EPA 1631 Hg B121708 09/18/2012 09/24/2012 1200736 Water EPA 1631 Hg B121743 09/20/2012 09/25/2012 1200740 Water EPA 1631 Hg

3958 6th Avenue NW Seattle WA 98107 ·P(206) 632-6206 ·F(206) 632-6017 ·brl@brooksrand.com ·www.brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Sample Results

Sample Sequence Result MDL MRL Unit Batch

Qualifier Analyte Basis

Report Matrix BioReactor 1 Inf 

### 1400 T 1200736 B121708 Influent 34.0 12.8 1237037-01 Hg ng/L 41.3 D H 1200736 B121708 Influent 2.02 0.76 1237037-02 Hg ng/L
BioReactor 1 Inf Hg Blk 

### 0.15 T U 1200724 B121708 DIW 0.41 0.15 1237037-03 Hg ng/L 0.16 D H, U 1200724 B121708 DIW 0.42 0.16 1237037-04 Hg ng/L

BioReactor 2 Eff 

### 23.7 T 1200736 B121708 Effluent 0.49 0.18 1237037-09 Hg ng/L 22.9 D H 1200740 B121743 Effluent 0.40 0.15 1237037-10 Hg ng/L
BioReactor 2 Eff Hg Blk 

### 0.16 T U 1200740 B121743 DIW 0.41 0.16 1237037-11 Hg ng/L 0.15 D H, U 1200740 B121743 DIW 0.41 0.15 1237037-12 Hg ng/L

BioReactor 2 Inf 

### 550 T 1200736 B121708 Influent 10.2 3.83 1237037-05 Hg ng/L 17.1 D H 1200736 B121708 Influent 0.42 0.16 1237037-06 Hg ng/L
BioReactor 2 Inf Hg Blk 

### 0.16 T U 1200724 B121708 DIW 0.42 0.16 1237037-07 Hg ng/L 0.15 D H, U 1200724 B121708 DIW 0.40 0.15 1237037-08 Hg ng/L

3958 6th Avenue NW Seattle WA 98107 ·P(206) 632-6206 ·F(206) 632-6017 ·brl@brooksrand.com ·www.brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Accuracy & Precision Summary

Batch: 

### B121708

Analyte Result Units Native Spike REC & Limits RPD & Limits Method: 

### EPA 1631 Lab Matrix: 

### Water

Sample Certified Reference Material (1237042, NIST 1641d 1000x dilution) B121708-SRM1 

### 62.72 110% ng/L 85-115 Hg 68.70
Certified Reference Material (1237042, NIST 1641d 1000x dilution) B121708-SRM2 

### 62.72 99% ng/L 85-115 Hg 61.78
Duplicate (1237021-02) B121708-DUP5 

### ng/L 240.5% Hg 5.63

5.66
Matrix Spike (1237021-02) B121708-MS5 

### 61.59 95% ng/L 71-125 Hg 64.13

5.66
Matrix Spike Duplicate (1237021-02) B121708-MSD5 

### 58.52 95% ng/L 71-125 245% Hg 61.11

5.66

3958 6th Avenue NW Seattle WA 98107 ·P(206) 632-6206 ·F(206) 632-6017 ·brl@brooksrand.com ·www.brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Accuracy & Precision Summary

Batch: 

### B121743

Analyte Result Units Native Spike REC & Limits RPD & Limits Method: 

### EPA 1631 Lab Matrix: 

### Water

Sample Certified Reference Material (1237042, NIST 1641d 1000x dilution) B121743-SRM1 

### 62.72 99% ng/L 85-115 Hg 62.13
Duplicate (1237041-03) B121743-DUP1 

### ng/L 2411% Hg 96.92

87.20
Matrix Spike (1237041-03) B121743-MS1 

### 1515 95% ng/L 71-125 Hg 1531

87.20
Matrix Spike Duplicate (1237041-03) B121743-MSD1 

### 1515 95% ng/L 71-125 24 0.001% Hg 1531

87.20

3958 6th Avenue NW Seattle WA 98107 ·P(206) 632-6206 ·F(206) 632-6017 ·brl@brooksrand.com ·www.brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Method Blanks & Reporting Limits

Batch: 

### B121708
Method: 

### EPA 1631 Matrix: 

### Water Analyte: 

### Hg Result Units Sample 

### B121708-BLK1 ng/L 0.27 B121708-BLK2 ng/L 0.13 B121708-BLK3 ng/L 0.12 B121708-BLK4 ng/L 0.14 MDL: 

### 0.16 Average: 

### 0.17 Standard Deviation: 

### 0.07 Limit: 

### 0.10 Limit: 

### 0.50 MRL: 

### 0.42

3958 6th Avenue NW Seattle WA 98107 ·P(206) 632-6206 ·F(206) 632-6017 ·brl@brooksrand.com ·www.brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Method Blanks & Reporting Limits

Batch: 

### B121743
Method: 

### EPA 1631 Matrix: 

### Water Analyte: 

### Hg Result Units Sample 

### B121743-BLK1 ng/L 0.13 B121743-BLK2 ng/L 0.18 B121743-BLK3 ng/L 0.16 B121743-BLK4 ng/L 0.14 MDL: 

### 0.16 Average: 

### 0.15 Standard Deviation: 

### 0.02 Limit: 

### 0.11 Limit: 

### 0.50 MRL: 

### 0.42

3958 6th Avenue NW Seattle WA 98107 ·P(206) 632-6206 ·F(206) 632-6017 ·brl@brooksrand.com ·www.brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Instrument Calibration

Sequence: 

### 1200724 Method: 

### EPA 1631 Instrument: 

### THG-05 Date: 

### 09/19/2012 Total Mercury and Mercury Speciation by CVAFS

Analyte: 

### Hg REC & Limits Result True Value Lab ID Units 

### 1200724-IBL1 0.55 pg of Hg 1200724-IBL2 0.59 pg of Hg 1200724-IBL3 0.85 pg of Hg 1200724-IBL4 1.49 pg of Hg 1200724-CAL1 10.00 8.44 pg of Hg 84% 1200724-CAL2 25.00 24.04 pg of Hg 96% 1200724-CAL3 100.0 100.6 pg of Hg 101% 1200724-CAL4 500.0 518.9 pg of Hg 104% 1200724-CAL5 2500 2773 pg of Hg 111% 1200724-CAL6 10000 10920 pg of Hg 109% 1200724-ICV1 1568 1718 pg of Hg 110% 85-115 1200724-CCB1 14.4 pg of Hg 1200724-CCV1 500.0 551.6 pg of Hg 110% 77-123 1200724-CCB2 6.70 pg of Hg 1200724-CCB3 4.20 pg of Hg 1200724-CCB4 4.65 pg of Hg 1200724-CCV2 500.0 551.2 pg of Hg 110% 77-123 1200724-CCB5 3.76 pg of Hg 1200724-CCV3 500.0 589.9 pg of Hg 118% 77-123 1200724-CCB6 9.75 pg of Hg 1200724-CCV4 500.0 615.6 pg of Hg 123% 77-123 1200724-CCB7 6.91 pg of Hg 1200724-CCV5 500.0 609.3 pg of Hg 122% 77-123 1200724-CCB8 6.61 pg of Hg 1200724-CCV6 500.0 603.8 pg of Hg 121% 77-123 1200724-CCB9 4.54 pg of Hg 1200724-CCV7 500.0 581.0 pg of Hg 116% 77-123 1200724-CCBA 4.28 pg of Hg 1200724-CCV8 500.0 564.1 pg of Hg 113% 77-123 1200724-CCBB 3.89 pg of Hg 1200724-CCV9 500.0 581.3 pg of Hg 116% 77-123 1200724-CCBC 6.47 pg of Hg

3958 6th Avenue NW Seattle WA 98107 ·P(206) 632-6206 ·F(206) 632-6017 ·brl@brooksrand.com ·www.brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Instrument Calibration

Sequence: 

### 1200736 Method: 

### EPA 1631 Instrument: 

### THG-05 Date: 

### 09/24/2012 Total Mercury and Mercury Speciation by CVAFS

Analyte: 

### Hg REC & Limits Result True Value Lab ID Units 

### 1200736-IBL1 1.30 pg of Hg 1200736-IBL2 1.05 pg of Hg 1200736-IBL3 2.65 pg of Hg 1200736-IBL4 2.88 pg of Hg 1200736-CAL1 10.00 10.53 pg of Hg 105% 1200736-CAL2 25.00 24.49 pg of Hg 98% 1200736-CAL3 100.0 96.17 pg of Hg 96% 1200736-CAL4 500.0 493.0 pg of Hg 99% 1200736-CAL5 2500 2617 pg of Hg 105% 1200736-CAL6 10000 9804 pg of Hg 98% 1200736-ICV1 1568 1545 pg of Hg 99% 85-115 1200736-CCB1 10.4 pg of Hg 1200736-CCV1 500.0 505.3 pg of Hg 101% 77-123 1200736-CCB2 5.42 pg of Hg 1200736-CCB3 4.57 pg of Hg 1200736-CCB4 5.91 pg of Hg 1200736-CCV2 500.0 501.5 pg of Hg 100% 77-123 1200736-CCB5 5.23 pg of Hg 1200736-CCV3 500.0 499.5 pg of Hg 100% 77-123 1200736-CCB6 4.64 pg of Hg 1200736-CCV4 500.0 504.1 pg of Hg 101% 77-123 1200736-CCB7 4.52 pg of Hg 1200736-CCV5 500.0 476.3 pg of Hg 95% 77-123 1200736-CCB8 4.57 pg of Hg 1200736-CCV6 500.0 471.7 pg of Hg 94% 77-123 1200736-CCB9 4.42 pg of Hg 1200736-CCV7 500.0 477.2 pg of Hg 95% 77-123 1200736-CCBA 4.55 pg of Hg 1200736-CCV8 500.0 488.6 pg of Hg 98% 77-123 1200736-CCBB 6.71 pg of Hg 1200736-CCV9 500.0 488.4 pg of Hg 98% 77-123 1200736-CCBC 7.59 pg of Hg 1200736-CCVA 500.0 500.5 pg of Hg 100% 77-123 1200736-CCBD 6.49 pg of Hg 1200736-CCVB 500.0 493.7 pg of Hg 99% 77-123 1200736-CCBE 9.03 pg of Hg 1200736-CCVC 500.0 485.7 pg of Hg 97% 77-123 1200736-CCBF 6.18 pg of Hg 1200736-CCVD 500.0 484.7 pg of Hg 97% 77-123 1200736-CCBG 4.09 pg of Hg

3958 6th Avenue NW Seattle WA 98107 ·P(206) 632-6206 ·F(206) 632-6017 ·brl@brooksrand.com ·www.brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Instrument Calibration

Sequence: 

### 1200740 Method: 

### EPA 1631 Instrument: 

### THG-05 Date: 

### 09/25/2012 Total Mercury and Mercury Speciation by CVAFS

Analyte: 

### Hg REC & Limits Result True Value Lab ID Units 

### 1200740-IBL1 1.40 pg of Hg 1200740-IBL2 1.85 pg of Hg 1200740-IBL3 2.50 pg of Hg 1200740-IBL4 4.25 pg of Hg 1200740-CAL1 10.00 10.11 pg of Hg 101% 1200740-CAL2 25.00 23.88 pg of Hg 96% 1200740-CAL3 100.0 99.90 pg of Hg 100% 1200740-CAL4 500.0 501.1 pg of Hg 100% 1200740-CAL5 2500 2574 pg of Hg 103% 1200740-CAL6 10000 10060 pg of Hg 101% 1200740-ICV1 1568 1553 pg of Hg 99% 85-115 1200740-CCB1 8.83 pg of Hg 1200740-CCV1 500.0 508.8 pg of Hg 102% 77-123 1200740-CCB2 7.14 pg of Hg 1200740-CCB3 4.51 pg of Hg 1200740-CCB4 4.97 pg of Hg 1200740-CCV2 500.0 499.8 pg of Hg 100% 77-123 1200740-CCB5 4.84 pg of Hg 1200740-CCV3 500.0 479.7 pg of Hg 96% 77-123 1200740-CCB6 5.48 pg of Hg 1200740-CCV4 500.0 473.9 pg of Hg 95% 77-123 1200740-CCB7 3.66 pg of Hg 1200740-CCV5 500.0 486.7 pg of Hg 97% 77-123 1200740-CCB8 3.81 pg of Hg 1200740-CCV6 500.0 439.8 pg of Hg 88% 77-123 1200740-CCB9 3.87 pg of Hg 1200740-CCV7 500.0 451.7 pg of Hg 90% 77-123 1200740-CCBA 3.43 pg of Hg 1200740-CCV8 500.0 497.9 pg of Hg 100% 77-123 1200740-CCBB 11.2 pg of Hg 1200740-CCV9 500.0 494.2 pg of Hg 99% 77-123 1200740-CCBC 7.75 pg of Hg 1200740-CCVA 500.0 496.0 pg of Hg 99% 77-123 1200740-CCBD 7.09 pg of Hg 1200740-CCVB 500.0 492.0 pg of Hg 98% 77-123 1200740-CCBE 6.35 pg of Hg

3958 6th Avenue NW Seattle WA 98107 ·P(206) 632-6206 ·F(206) 632-6017 ·brl@brooksrand.com ·www.brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Sample Containers

Lab ID: 

### 1237037-01 Report Matrix: 

### Influent Sample Type: 

### Sample Received: 

### 09/14/2012 Sample: 

### BioReactor 1 Inf Collected: 

### 09/11/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71666330 10 Cooler

none n/a 500 mL Bottle FLPE Hg-T

Lab ID: 

### 1237037-02 Report Matrix: 

### Influent Sample Type: 

### Sample Received: 

### 09/14/2012 Sample: 

### BioReactor 1 Inf Collected: 

### 09/11/2012
Comments: 

### Qualify H Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71659890 20 Cooler

none n/a 250 mL Bottle FLPE Hg-T
Comments: 

### Split from THg Container

Lab ID: 

### 1237037-03 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 09/14/2012 Sample: 

### BioReactor 1 Inf Hg Blk Collected: 

### 09/11/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71666330 10 Cooler

none n/a 500 mL Bottle FLPE Hg-T

Lab ID: 

### 1237037-04 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 09/14/2012 Sample: 

### BioReactor 1 Inf Hg Blk Collected: 

### 09/11/2012
Comments: 

### Qualify H Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71659890 20 Cooler

none n/a 250 mL Bottle FLPE Hg-T
Comments: 

### Split from THg Container

Lab ID: 

### 1237037-05 Report Matrix: 

### Influent Sample Type: 

### Sample Received: 

### 09/14/2012 Sample: 

### BioReactor 2 Inf Collected: 

### 09/11/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71666330 10 Cooler

none n/a 500 mL Bottle FLPE Hg-T

3958 6th Avenue NW Seattle WA 98107 ·P(206) 632-6206 ·F(206) 632-6017 ·brl@brooksrand.com ·www.brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Sample Containers

Lab ID: 

### 1237037-06 Report Matrix: 

### Influent Sample Type: 

### Sample Received: 

### 09/14/2012 Sample: 

### BioReactor 2 Inf Collected: 

### 09/11/2012
Comments: 

### Qualify H Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71659890 20 Cooler

none n/a 250 mL Bottle FLPE Hg-T
Comments: 

### Split from THg Container

Lab ID: 

### 1237037-07 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 09/14/2012 Sample: 

### BioReactor 2 Inf Hg Blk Collected: 

### 09/11/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71666330 10 Cooler

none n/a 500 mL Bottle FLPE Hg-T

Lab ID: 

### 1237037-08 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 09/14/2012 Sample: 

### BioReactor 2 Inf Hg Blk Collected: 

### 09/11/2012
Comments: 

### Qualify H Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71659890 20 Cooler

none n/a 250 mL Bottle FLPE Hg-T
Comments: 

### Split from THg Container

Lab ID: 

### 1237037-09 Report Matrix: 

### Effluent Sample Type: 

### Sample Received: 

### 09/14/2012 Sample: 

### BioReactor 2 Eff Collected: 

### 09/11/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71666330 10 Cooler

none n/a 500 mL Bottle FLPE Hg-T

Lab ID: 

### 1237037-10 Report Matrix: 

### Effluent Sample Type: 

### Sample Received: 

### 09/14/2012 Sample: 

### BioReactor 2 Eff Collected: 

### 09/11/2012
Comments: 

### Qualify H Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71659890 20 Cooler

none n/a 250 mL Bottle FLPE Hg-T
Comments: 

### Split from THg Container

3958 6th Avenue NW Seattle WA 98107 ·P(206) 632-6206 ·F(206) 632-6017 ·brl@brooksrand.com ·www.brooksrand.comProject ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Sample Containers

Lab ID: 

### 1237037-11 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 09/14/2012 Sample: 

### BioReactor 2 Eff Hg Blk Collected: 

### 09/11/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71666330 10 Cooler

none n/a 500 mL Bottle FLPE Hg-T

Lab ID: 

### 1237037-12 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 09/14/2012 Sample: 

### BioReactor 2 Eff Hg Blk Collected: 

### 09/11/2012
Comments: 

### Qualify H Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71659890 20 Cooler

none n/a 250 mL Bottle FLPE Hg-T
Comments: 

### Split from THg Container



# Shipping Containers

Cooler Tracking No: 

### 5353 0519 4152 via FedEx Temperature: 

### 0.2 °C Coolant Type: 

### Ice Description: 

### Cooler Damaged in transit? 

### No Returned to client? 

### No Custody seals present? 

### No Custody seals intact? 

### No COC present? 

### Yes Received: 

### September 14, 2012 9:00

3958 6th Avenue NW Seattle WA 98107 ·P(206) 632-6206 ·F(206) 632-6017 ·brl@brooksrand.com ·www.brooksrand.com

### This data package contains the analytical results for the project identified above and includes a Case Narrative, Sample Results and Chain of Custody. Unless otherwise noted, all samples were received in acceptable condition and processed according to the referenced methods. Data qualifiers are flagged individually on each sample. A key reference for the data qualifiers appears at the end of this case narrative. NC Certification No. 402 SC Certification No. 99012 NC Drinking Water Cert No. 37735 

# Case Narrative 09/19/2012

Duke Energy Corporation (04) Jay Perkins 13339 Hagers Ferry Road Huntersville, NC 28078 Project: Allen Shay/Miller Creek Project No.: J12090176 Lab Submittal Date: 09/12/2012 Prism Work Order: 2090231 VA Certification No. 1287



### Please call if you have any questions relating to this analytical report. Respectfully, PRISM LABORATORIES, INC.



### VP Laboratory Services Reviewed By

Data Qualifiers Key Reference: 

### HT Sample received and analyzed outside of the hold time.

Method Detection Limit MDL Below Reporting Limit BRL
Relative Percent Difference RPD


## * Results reported to the reporting limit. All other results are reported to the MDL with values between MDL and reporting limit indicated with a J.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 1 of 9

# Sample Receipt Summary 09/19/2012 

### Prism Work Order: 2090231 Client Sample ID Matrix Lab Sample ID Date Sampled Date Received 

### 2090231-01 09/11/12 2012019722/FGD Purge Eff 09/12/12 Water 2090231-02 09/11/12 2012019724/BioReactor 1 Inf 09/12/12 Water 2090231-03 09/11/12 2012019726/BioReactor 2 Inf 09/12/12 Water 2090231-04 09/11/12 2012019728/BioReactor 2 Eff 09/12/12 Water
Samples received in good condition at 2.4 degrees C unless otherwise noted.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 2 of 9

# Laboratory Report 09/19/2012

Result Parameter Batch ID Report Limit Analysis Date/Time Method Dilution Factor Units MDL Analyst 

### 13339 Hagers Ferry Road Project No.: Project: Allen Shay/Miller Creek Duke Energy Corporation (04)

Huntersville, NC 28078 Attn: Jay Perkins Sample Matrix: Water Client Sample ID: Prism Sample ID: 2090231-01 Prism Work Order: 2090231 Time Collected: 09/11/12 03:30 Time Submitted: 09/12/12 15:00 2012019722/FGD Purge Eff


### J12090176

General Chemistry Parameters 6.9 *SM4500-H B P2I0244 1 9/14/12 13:30 JAB HT pH Units pH 31 *SM2320 B P2I0245 1 5.0 9/14/12 13:30 JAB mg/L 0.66 Total Alkalinity BRL *SM2320 B P2I0246 1 5.0 9/14/12 13:30 JAB mg/L 0.66 Carbonate Alkalinity 31 *SM2320 B P2I0247 1 5.0 9/14/12 13:30 JAB mg/L 0.66 Bicarbonate Alkalinity

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc. 449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 3 of 9

# Laboratory Report 09/19/2012

Result Parameter Batch ID Report Limit Analysis Date/Time Method Dilution Factor Units MDL Analyst 

### 13339 Hagers Ferry Road Project No.: Project: Allen Shay/Miller Creek Duke Energy Corporation (04)

Huntersville, NC 28078 Attn: Jay Perkins Sample Matrix: Water Client Sample ID: Prism Sample ID: 2090231-02 Prism Work Order: 2090231 Time Collected: 09/11/12 08:15 Time Submitted: 09/12/12 15:00 2012019724/BioReactor 1 Inf


### J12090176

General Chemistry Parameters 6.9 *SM4500-H B P2I0244 1 9/14/12 13:30 JAB HT pH Units pH 28 *SM2320 B P2I0245 1 5.0 9/14/12 13:30 JAB mg/L 0.66 Total Alkalinity BRL *SM2320 B P2I0246 1 5.0 9/14/12 13:30 JAB mg/L 0.66 Carbonate Alkalinity 28 *SM2320 B P2I0247 1 5.0 9/14/12 13:30 JAB mg/L 0.66 Bicarbonate Alkalinity

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc. 449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 4 of 9

# Laboratory Report 09/19/2012

Result Parameter Batch ID Report Limit Analysis Date/Time Method Dilution Factor Units MDL Analyst 

### 13339 Hagers Ferry Road Project No.: Project: Allen Shay/Miller Creek Duke Energy Corporation (04)

Huntersville, NC 28078 Attn: Jay Perkins Sample Matrix: Water Client Sample ID: Prism Sample ID: 2090231-03 Prism Work Order: 2090231 Time Collected: 09/11/12 08:27 Time Submitted: 09/12/12 15:00 2012019726/BioReactor 2 Inf


### J12090176

General Chemistry Parameters 6.7 *SM4500-H B P2I0244 1 9/14/12 13:30 JAB HT pH Units pH 210 *SM2320 B P2I0245 1 5.0 9/14/12 13:30 JAB mg/L 0.66 Total Alkalinity BRL *SM2320 B P2I0246 1 5.0 9/14/12 13:30 JAB mg/L 0.66 Carbonate Alkalinity 210 *SM2320 B P2I0247 1 5.0 9/14/12 13:30 JAB mg/L 0.66 Bicarbonate Alkalinity

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc. 449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 5 of 9

# Laboratory Report 09/19/2012

Result Parameter Batch ID Report Limit Analysis Date/Time Method Dilution Factor Units MDL Analyst 

### 13339 Hagers Ferry Road Project No.: Project: Allen Shay/Miller Creek Duke Energy Corporation (04)

Huntersville, NC 28078 Attn: Jay Perkins Sample Matrix: Water Client Sample ID: Prism Sample ID: 2090231-04 Prism Work Order: 2090231 Time Collected: 09/11/12 08:23 Time Submitted: 09/12/12 15:00 2012019728/BioReactor 2 Eff


### J12090176

General Chemistry Parameters 6.9 *SM4500-H B P2I0244 1 9/14/12 13:30 JAB HT pH Units pH 130 *SM2320 B P2I0245 1 5.0 9/14/12 13:30 JAB mg/L 0.66 Total Alkalinity BRL *SM2320 B P2I0246 1 5.0 9/14/12 13:30 JAB mg/L 0.66 Carbonate Alkalinity 130 *SM2320 B P2I0247 1 5.0 9/14/12 13:30 JAB mg/L 0.66 Bicarbonate Alkalinity

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc. 449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 6 of 99/19/12 

### Level II QC Report

13339 Hagers Ferry Road Project No: J12090176 Project: Allen Shay/Miller Creek Duke Energy Corporation (04)

Huntersville, NC 28078 Attn: Jay Perkins Prism Work Order: 2090231 Time Submitted: 9/12/2012 3:00:00PM

Result Limit

Reporting Units Level Spike Result

Source %REC %REC Limits RPD RPD Limit Notes Analyte General Chemistry Parameters - Quality Control

Batch P2I0244 - NO PREP LCS (P2I0244-BS1) Prepared & Analyzed: 09/14/12 pH pH Units 6.82 6.860 99-101 99 Batch P2I0245 - NO PREP Blank (P2I0245-BLK1) Prepared & Analyzed: 09/14/12 Total Alkalinity mg/L BRL 5.0 LCS (P2I0245-BS1) Prepared & Analyzed: 09/14/12 Total Alkalinity mg/L 243 5.0 250.0 90-110 97 LCS Dup (P2I0245-BSD1) Prepared & Analyzed: 09/14/12 Total Alkalinity mg/L 248 5.0 250.0 200 90-110 99 2 Batch P2I0246 - NO PREP Blank (P2I0246-BLK1) Prepared & Analyzed: 09/14/12 Carbonate Alkalinity mg/L BRL 5.0 LCS (P2I0246-BS1) Prepared & Analyzed: 09/14/12 Carbonate Alkalinity mg/L 243 5.0 90-110 LCS Dup (P2I0246-BSD1) Prepared & Analyzed: 09/14/12 Carbonate Alkalinity mg/L 248 5.0 200 90-110 2 Batch P2I0247 - NO PREP Blank (P2I0247-BLK1) Prepared & Analyzed: 09/14/12 Bicarbonate Alkalinity mg/L BRL 5.0

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 7 of 99/19/12 

### Level II QC Report

13339 Hagers Ferry Road Project No: J12090176 Project: Allen Shay/Miller Creek Duke Energy Corporation (04)

Huntersville, NC 28078 Attn: Jay Perkins Prism Work Order: 2090231 Time Submitted: 9/12/2012 3:00:00PM

Result Limit

Reporting Units Level Spike Result

Source %REC %REC Limits RPD RPD Limit Notes Analyte General Chemistry Parameters - Quality Control

Batch P2I0247 - NO PREP LCS (P2I0247-BS1) Prepared & Analyzed: 09/14/12 Bicarbonate Alkalinity mg/L 243 5.0 250.0 90-110 97 LCS Dup (P2I0247-BSD1) Prepared & Analyzed: 09/14/12 Bicarbonate Alkalinity mg/L 248 5.0 250.0 200 90-110 99 2

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 8 of 9

### Page 9 of 9