### 13339 Hagers Ferry Road Huntersville, NC 28078-7929 McGuire Nuclear Complex - MG03A2 Phone: 980-875-5245 Fax: 980-875-4349 

# Order Summary Report 

# Analytical Laboratory

Order Number: J12040052 Project Name: Somerville Waste Water

Lab Contact: Jason C Perkins 

### Date: 4/16/2012 Customer Address: 253 Plant Allen Road Customer Name(s): BILL KENNEDY, RON LAWS, ALLEN STOWE, ROBBIN JOLLY, DON SCRUG

Belmont, NC 28012

Phone: 980-875-5348 Report Authorized By: (Signature)


### Program Comments: Please contact the Program Manager (Jason C Perkins) with any questions regarding this report. Data Flags & Calculations: Any analytical tests or individual analytes within a test flagged with a Qualifier indicate a deviation from the method quality system or quality control requirement. The qualifier description is found at the end of the Certificate of Analysis (sample results) under the qualifiers heading. All results are reported on a dry weight basis unless otherwise noted. Data Package: This data package includes analytical results that are applicable only to the samples described in this narrative. An estimation of the uncertainty of measurement for the results in the report is available upon request. This report shall not be reproduced, except in full, without the written consent of the Analytical Laboratory. Please contact the Analytical laboratory with any questions. The order of individual sections within this report is as follows: Job Summary Report, Sample Identification, Technical Validation of Data Package, Analytical Laboratory Certificate of Analysis, Analytical Laboratory QC Reports, Sub-contracted Laboratory Results, Customer Specific Data Sheets, Reports & Documentation, Customer Database Entries, Test Case Narratives, Chain of Custody (COC) Certification: The Analytical Laboratory holds the following State Certifications : North Carolina (DENR) Certificate #248, South Carolina (DHEC) Laboratory ID # 99005. Contact the Analytical Laboratory for definitive information about the certification status of specific methods.

## Sample ID's & Descriptions:

Sample ID Plant/Station Collection Date and Time Collected By Sample Description 2012007680 ALLEN 30-Mar-12 9:30 AM BILL HASKINS FGD Purge Eff 2012007681 ALLEN 30-Mar-12 9:38 AM BILL HASKINS EQ Tank Eff 2012007682 ALLEN 30-Mar-12 9:35 AM BILL HASKINS BioReactor 1 Inf 2012007683 ALLEN 30-Mar-12 4:40 PM BILL HASKINS BioReactor 1 Inf BLANK 2012007684 ALLEN 30-Mar-12 9:46 AM BILL HASKINS BioReactor 2 Inf 2012007685 ALLEN 30-Mar-12 4:50 PM BILL HASKINS BioReactor 2 Inf BLANK 2012007686 ALLEN 30-Mar-12 9:42 AM BILL HASKINS BioReactor 2 Eff 2012007687 ALLEN 30-Mar-12 4:44 PM BILL HASKINS BioReactor 2 Eff BLANK 2012007688 ALLEN 30-Mar-12 BILL HASKINS Filter Blk 9 Total Samples

### COC and .pdf report are in agreement with sample totals

and analyses (compliance programs and procedures). All Results are less than the laboratory reporting limits. All laboratory QA/QC requirements are acceptable. The Vendor Laboratories have been qualified by the Analytical Laboratory Yes No



# Technical Validation Review Checklist:

Yes No

Yes No



## Report Sections Included:

Job Summary Report Sub-contracted Laboratory Results Sample Identification Customer Specific Data Sheets, Reports, & Documentation Technical Validation of Data Package Customer Database Entries Analytical Laboratory Certificate of Analysis Analytical Laboratory QC Report Chain of Custody

Reviewed By: 

### DataBase Administrator Date: 

### 4/16/2012 Yes

Electronic Data Deliverable (EDD) Sent Separately

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12040052

2012007680 Collection Date: 30-Mar-12 9:30 AM 

### Site: FGD Purge Eff Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF ALKALINITY Vendor Parameter Complete V_PRISM 1 INORGANIC IONS BY IC Bromide 580 mg/L EPA 300.0 10-Apr-12 13:51 JAHERMA 50 500 Chloride 2500 mg/L EPA 300.0 10-Apr-12 13:51 JAHERMA 50 500 Sulfate 1300 mg/L EPA 300.0 10-Apr-12 13:51 JAHERMA 50 500 MERCURY (COLD VAPOR) IN WATER Mercury (Hg) 37.2 ug/L EPA 245.1 05-Apr-12 13:35 AGIBBS 2.5 50 TOTAL RECOVERABLE METALS BY ICP Boron (B) 64.1 mg/L EPA 200.7 11-Apr-12 11:40 DJSULL1 0.5 10 Calcium (Ca) 2210 mg/L EPA 200.7 11-Apr-12 11:40 DJSULL1 0.1 10 Iron (Fe) 104 mg/L EPA 200.7 11-Apr-12 11:40 DJSULL1 0.1 10 Lithium (Li) 0.209 mg/L EPA 200.7 11-Apr-12 11:40 DJSULL1 0.05 10 Magnesium (Mg) 484 mg/L EPA 200.7 11-Apr-12 11:40 DJSULL1 0.05 10 Manganese (Mn) 7.54 mg/L EPA 200.7 11-Apr-12 11:40 DJSULL1 0.05 10 Potassium (K) 33.3 mg/L EPA 200.7 11-Apr-12 11:40 DJSULL1 1 10 Sodium (Na) 30.0 mg/L EPA 200.7 11-Apr-12 11:40 DJSULL1 0.5 10 DISSOLVED METALS BY ICP-MS Manganese (Mn) 6480 ug/L EPA 200.8 14-Apr-12 01:51 DJSULL1 20 20 Selenium (Se) 364 ug/L EPA 200.8 14-Apr-12 01:51 DJSULL1 20 20 TOTAL RECOVERABLE METALS BY ICP-MS Arsenic (As) 194 ug/L EPA 200.8 11-Apr-12 23:00 KRICHAR 10 10 Cadmium (Cd) < 10 ug/L EPA 200.8 11-Apr-12 23:00 KRICHAR 10 10 Chromium (Cr) 187 ug/L EPA 200.8 11-Apr-12 23:00 KRICHAR 10 10 Copper (Cu) 234 ug/L EPA 200.8 11-Apr-12 23:00 KRICHAR 10 10 Nickel (Ni) 258 ug/L EPA 200.8 11-Apr-12 23:00 KRICHAR 10 10 Selenium (Se) 3110 ug/L EPA 200.8 11-Apr-12 23:00 KRICHAR 10 10 Silver (Ag) < 10 ug/L EPA 200.8 11-Apr-12 23:00 KRICHAR 10 10 Zinc (Zn) 347 ug/L EPA 200.8 11-Apr-12 23:00 KRICHAR 10 10 SELENIUM SPECIATION Vendor Parameter Complete V_AS&C 1 TOTAL DISSOLVED SOLIDS Vendor Parameter Complete V_PACE 1

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12040052

2012007680 Collection Date: 30-Mar-12 9:30 AM 

### Site: FGD Purge Eff Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF TOTAL SUSPENDED SOLIDS Vendor Parameter Complete V_PACE 1


### 2012007681 Collection Date: 30-Mar-12 9:38 AM 

### Site: EQ Tank Eff Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF MERCURY (COLD VAPOR) IN WATER Mercury (Hg) 13.4 ug/L EPA 245.1 05-Apr-12 13:47 AGIBBS 2.5 50 TOTAL RECOVERABLE METALS BY ICP Boron (B) 19.1 mg/L EPA 200.7 11-Apr-12 11:44 DJSULL1 0.5 10 Calcium (Ca) 961 mg/L EPA 200.7 11-Apr-12 11:44 DJSULL1 0.1 10 Iron (Fe) 41.5 mg/L EPA 200.7 11-Apr-12 11:44 DJSULL1 0.1 10 Lithium (Li) 0.086 mg/L EPA 200.7 11-Apr-12 11:44 DJSULL1 0.05 10 Magnesium (Mg) 178 mg/L EPA 200.7 11-Apr-12 11:44 DJSULL1 0.05 10 Manganese (Mn) 2.64 mg/L EPA 200.7 11-Apr-12 11:44 DJSULL1 0.05 10 Potassium (K) 14.7 mg/L EPA 200.7 11-Apr-12 11:44 DJSULL1 1 10 Sodium (Na) 15.3 mg/L EPA 200.7 11-Apr-12 11:44 DJSULL1 0.5 10 DISSOLVED METALS BY ICP-MS Manganese (Mn) 2270 ug/L EPA 200.8 14-Apr-12 01:41 DJSULL1 10 10 Selenium (Se) 114 ug/L EPA 200.8 14-Apr-12 01:41 DJSULL1 10 10 TOTAL RECOVERABLE METALS BY ICP-MS Arsenic (As) 95.2 ug/L EPA 200.8 11-Apr-12 23:03 KRICHAR 10 10 Cadmium (Cd) < 10 ug/L EPA 200.8 11-Apr-12 23:03 KRICHAR 10 10 Chromium (Cr) 74.1 ug/L EPA 200.8 11-Apr-12 23:03 KRICHAR 10 10 Copper (Cu) 101 ug/L EPA 200.8 11-Apr-12 23:03 KRICHAR 10 10 Nickel (Ni) 108 ug/L EPA 200.8 11-Apr-12 23:03 KRICHAR 10 10 Selenium (Se) 1920 ug/L EPA 200.8 11-Apr-12 23:03 KRICHAR 10 10 Silver (Ag) < 10 ug/L EPA 200.8 11-Apr-12 23:03 KRICHAR 10 10 Zinc (Zn) 151 ug/L EPA 200.8 11-Apr-12 23:03 KRICHAR 10 10


### 2012007682 Collection Date: 30-Mar-12 9:35 AM 

### Site: BioReactor 1 Inf Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF ALKALINITY Vendor Parameter Complete V_PRISM 1

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12040052

2012007682 Collection Date: 30-Mar-12 9:35 AM 

### Site: BioReactor 1 Inf Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF MERCURY 1631 Vendor Parameter Complete V_BRAND 1 MERCURY 1631 - DISSOLVED Vendor Parameter Complete V_BRAND 1 TOTAL RECOVERABLE METALS BY ICP Boron (B) 22.6 mg/L EPA 200.7 11-Apr-12 11:48 DJSULL1 0.5 10 Calcium (Ca) 1430 mg/L EPA 200.7 11-Apr-12 11:48 DJSULL1 0.1 10 Iron (Fe) < 0.1 mg/L EPA 200.7 11-Apr-12 11:48 DJSULL1 0.1 10 Lithium (Li) < 0.05 mg/L EPA 200.7 11-Apr-12 11:48 DJSULL1 0.05 10 Magnesium (Mg) 240 mg/L EPA 200.7 11-Apr-12 11:48 DJSULL1 0.05 10 Manganese (Mn) 0.534 mg/L EPA 200.7 11-Apr-12 11:48 DJSULL1 0.05 10 Potassium (K) 9.78 mg/L EPA 200.7 11-Apr-12 11:48 DJSULL1 1 10 Sodium (Na) 19.4 mg/L EPA 200.7 11-Apr-12 11:48 DJSULL1 0.5 10 DISSOLVED METALS BY ICP-MS Manganese (Mn) 507 ug/L EPA 200.8 14-Apr-12 01:45 DJSULL1 10 10 Selenium (Se) 149 ug/L EPA 200.8 14-Apr-12 01:45 DJSULL1 10 10 TOTAL RECOVERABLE METALS BY ICP-MS Arsenic (As) < 10 ug/L EPA 200.8 11-Apr-12 23:06 KRICHAR 10 10 Cadmium (Cd) < 10 ug/L EPA 200.8 11-Apr-12 23:06 KRICHAR 10 10 Chromium (Cr) < 10 ug/L EPA 200.8 11-Apr-12 23:06 KRICHAR 10 10 Copper (Cu) < 10 ug/L EPA 200.8 11-Apr-12 23:06 KRICHAR 10 10 Nickel (Ni) 11.3 ug/L EPA 200.8 11-Apr-12 23:06 KRICHAR 10 10 Selenium (Se) 192 ug/L EPA 200.8 11-Apr-12 23:06 KRICHAR 10 10 Silver (Ag) < 10 ug/L EPA 200.8 11-Apr-12 23:06 KRICHAR 10 10 Zinc (Zn) < 10 ug/L EPA 200.8 11-Apr-12 23:06 KRICHAR 10 10 SELENIUM SPECIATION Vendor Parameter Complete V_AS&C 1


### 2012007683 Collection Date: 30-Mar-12 4:40 PM 

### Site: BioReactor 1 Inf BLANK Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF MERCURY 1631 Vendor Parameter Complete V_BRAND 1 MERCURY 1631 - DISSOLVED Vendor Parameter Complete V_BRAND 1

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12040052

2012007684 Collection Date: 30-Mar-12 9:46 AM 

### Site: BioReactor 2 Inf Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF ALKALINITY Vendor Parameter Complete V_PRISM 1 MERCURY 1631 Vendor Parameter Complete V_BRAND 1 MERCURY 1631 - DISSOLVED Vendor Parameter Complete V_BRAND 1 TOTAL RECOVERABLE METALS BY ICP Boron (B) 21.5 mg/L EPA 200.7 11-Apr-12 11:52 DJSULL1 0.5 10 Calcium (Ca) 1390 mg/L EPA 200.7 11-Apr-12 11:52 DJSULL1 0.1 10 Iron (Fe) 5.22 mg/L EPA 200.7 11-Apr-12 11:52 DJSULL1 0.1 10 Lithium (Li) < 0.05 mg/L EPA 200.7 11-Apr-12 11:52 DJSULL1 0.05 10 Magnesium (Mg) 224 mg/L EPA 200.7 11-Apr-12 11:52 DJSULL1 0.05 10 Manganese (Mn) 0.671 mg/L EPA 200.7 11-Apr-12 11:52 DJSULL1 0.05 10 Potassium (K) 11.2 mg/L EPA 200.7 11-Apr-12 11:52 DJSULL1 1 10 Sodium (Na) 18.6 mg/L EPA 200.7 11-Apr-12 11:52 DJSULL1 0.5 10 DISSOLVED METALS BY ICP-MS Manganese (Mn) 624 ug/L EPA 200.8 14-Apr-12 01:55 DJSULL1 10 10 Selenium (Se) 21.1 ug/L EPA 200.8 14-Apr-12 01:55 DJSULL1 10 10 TOTAL RECOVERABLE METALS BY ICP-MS Arsenic (As) < 10 ug/L EPA 200.8 11-Apr-12 23:09 KRICHAR 10 10 Cadmium (Cd) < 10 ug/L EPA 200.8 11-Apr-12 23:09 KRICHAR 10 10 Chromium (Cr) < 10 ug/L EPA 200.8 11-Apr-12 23:09 KRICHAR 10 10 Copper (Cu) < 10 ug/L EPA 200.8 11-Apr-12 23:09 KRICHAR 10 10 Nickel (Ni) < 10 ug/L EPA 200.8 11-Apr-12 23:09 KRICHAR 10 10 Selenium (Se) 18.8 ug/L EPA 200.8 11-Apr-12 23:09 KRICHAR 10 10 Silver (Ag) < 10 ug/L EPA 200.8 11-Apr-12 23:09 KRICHAR 10 10 Zinc (Zn) < 10 ug/L EPA 200.8 11-Apr-12 23:09 KRICHAR 10 10 SELENIUM SPECIATION Vendor Parameter Complete V_AS&C 1


### 2012007685 Collection Date: 30-Mar-12 4:50 PM 

### Site: BioReactor 2 Inf BLANK Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF MERCURY 1631 Vendor Parameter Complete V_BRAND 1

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12040052

2012007685 Collection Date: 30-Mar-12 4:50 PM 

### Site: BioReactor 2 Inf BLANK Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF MERCURY 1631 - DISSOLVED Vendor Parameter Complete V_BRAND 1


### 2012007686 Collection Date: 30-Mar-12 9:42 AM 

### Site: BioReactor 2 Eff Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF ALKALINITY Vendor Parameter Complete V_PRISM 1 INORGANIC IONS BY IC Bromide 480 mg/L EPA 300.0 10-Apr-12 12:21 JAHERMA 50 500 Chloride 1700 mg/L EPA 300.0 10-Apr-12 12:21 JAHERMA 50 500 Sulfate 1300 mg/L EPA 300.0 10-Apr-12 12:21 JAHERMA 50 500 MERCURY 1631 Vendor Parameter Complete V_BRAND 1 MERCURY 1631 - DISSOLVED Vendor Parameter Complete V_BRAND 1 TOTAL RECOVERABLE METALS BY ICP Boron (B) 26.1 mg/L EPA 200.7 11-Apr-12 11:56 DJSULL1 0.5 10 Calcium (Ca) 1310 mg/L EPA 200.7 11-Apr-12 11:56 DJSULL1 0.1 10 Iron (Fe) 0.148 mg/L EPA 200.7 11-Apr-12 11:56 DJSULL1 0.1 10 Lithium (Li) < 0.05 mg/L EPA 200.7 11-Apr-12 11:56 DJSULL1 0.05 10 Magnesium (Mg) 203 mg/L EPA 200.7 11-Apr-12 11:56 DJSULL1 0.05 10 Manganese (Mn) 0.792 mg/L EPA 200.7 11-Apr-12 11:56 DJSULL1 0.05 10 Potassium (K) 12.5 mg/L EPA 200.7 11-Apr-12 11:56 DJSULL1 1 10 Sodium (Na) 16.9 mg/L EPA 200.7 11-Apr-12 11:56 DJSULL1 0.5 10 DISSOLVED METALS BY ICP-MS Manganese (Mn) 724 ug/L EPA 200.8 14-Apr-12 01:59 DJSULL1 5 5 Selenium (Se) 8.95 ug/L EPA 200.8 14-Apr-12 01:59 DJSULL1 5 5

# Certificate of Laboratory Analysis This report shall not be reproduced, except in full.



## Order # J12040052

2012007686 Collection Date: 30-Mar-12 9:42 AM 

### Site: BioReactor 2 Eff Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF TOTAL RECOVERABLE METALS BY ICP-MS Arsenic (As) < 5 ug/L EPA 200.8 11-Apr-12 23:13 KRICHAR 5 5 Cadmium (Cd) < 5 ug/L EPA 200.8 11-Apr-12 23:13 KRICHAR 5 5 Chromium (Cr) < 5 ug/L EPA 200.8 11-Apr-12 23:13 KRICHAR 5 5 Copper (Cu) < 5 ug/L EPA 200.8 11-Apr-12 23:13 KRICHAR 5 5 Nickel (Ni) 28.8 ug/L EPA 200.8 11-Apr-12 23:13 KRICHAR 5 5 Selenium (Se) 9.49 ug/L EPA 200.8 11-Apr-12 23:13 KRICHAR 5 5 Silver (Ag) < 5 ug/L EPA 200.8 11-Apr-12 23:13 KRICHAR 5 5 Zinc (Zn) < 5 ug/L EPA 200.8 11-Apr-12 23:13 KRICHAR 5 5 SELENIUM SPECIATION Vendor Parameter Complete V_AS&C 1


### 2012007687 Collection Date: 30-Mar-12 4:44 PM 

### Site: BioReactor 2 Eff BLANK Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF MERCURY 1631 Vendor Parameter Complete V_BRAND 1 MERCURY 1631 - DISSOLVED Vendor Parameter Complete V_BRAND 1


### 2012007688 Collection Date: 30-Mar-12 

### Site: Filter Blk Matrix: OTHER

Analyte Analysis Date/Time Method Units Qualifiers RDL Result 

### Sample #:

Analyst DF DISSOLVED METALS BY ICP-MS Manganese (Mn) < 1 ug/L EPA 200.8 14-Apr-12 01:13 DJSULL1 1 1 Selenium (Se) < 1 ug/L EPA 200.8 14-Apr-12 01:13 DJSULL1 1 1

### This data package contains the analytical results for the project identified above and includes a Case Narrative, Sample Results and Chain of Custody. Unless otherwise noted, all samples were received in acceptable condition and processed according to the referenced methods. Data qualifiers are flagged individually on each sample. A key reference for the data qualifiers appears at the end of this case narrative. NC Certification No. 402 SC Certification No. 99012 NC Drinking Water Cert No. 37735 

# Case Narrative 04/10/2012

Duke Energy Corporation (04) Jay Perkins 13339 Hagers Ferry Road Huntersville, NC 28078 Project: Allen 50/50 Somerville-Wild Boar/Miller Creek Project No.: J12040052 Lab Submittal Date: 04/03/2012 Prism Work Order: 2040051 VA Certification No. 1287



### Please call if you have any questions relating to this analytical report. Respectfully, PRISM LABORATORIES, INC.



### VP Laboratory Services Reviewed By

Data Qualifiers Key Reference: 

### HT Sample received and analyzed outside of the hold time.

Method Detection Limit MDL Below Reporting Limit BRL
Relative Percent Difference RPD


## * Results reported to the reporting limit. All other results are reported to the MDL with values between MDL and reporting limit indicated with a J.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 1 of 9

# Sample Receipt Summary 04/10/2012 

## Prism Work Order: 2040051 Client Sample ID Matrix Lab Sample ID Date Sampled Date Received 

### 2040051-01 03/30/12 2012007680/FGD Purge Eff 04/03/12 Water 2040051-02 03/30/12 2012007682/BioReactor 1 Inf 04/03/12 Water 2040051-03 03/30/12 2012007684/BioReactor 2 Inf 04/03/12 Water 2040051-04 03/30/12 2012007686/BioReactor 2 Eff 04/03/12 Water Samples received in good condition at 3.2 degrees C unless otherwise noted.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 2 of 9

# Laboratory Report 04/10/2012

Result Parameter Batch ID Report Limit Analysis Date/Time Method Dilution Factor Units MDL Analyst 

### 13339 Hagers Ferry Road Project No.: Project: Allen 50/50 Somerville-Wild Boar/Miller Creek Duke Energy Corporation (04)

Huntersville, NC 28078 Attn: Jay Perkins Sample Matrix: Water Client Sample ID: Prism Sample ID: 2040051-01 Prism Work Order: 2040051 Time Collected: 03/30/12 09:30 Time Submitted: 04/03/12 16:40 2012007680/FGD Purge Eff


### J12040052

General Chemistry Parameters 7.0 *SM4500-H B P2D0057 1 4/4/12 11:30 JAB HT pH Units pH 38 *SM2320 B P2D0088 1 5.0 4/5/12 12:45 JAB mg/L 0.66 Total Alkalinity BRL *SM2320 B P2D0089 1 5.0 4/5/12 12:45 JAB mg/L 0.66 Carbonate Alkalinity 38 *SM2320 B P2D0090 1 5.0 4/5/12 12:45 JAB mg/L 0.66 Bicarbonate Alkalinity

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc. 449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 3 of 9

# Laboratory Report 04/10/2012

Result Parameter Batch ID Report Limit Analysis Date/Time Method Dilution Factor Units MDL Analyst 

### 13339 Hagers Ferry Road Project No.: Project: Allen 50/50 Somerville-Wild Boar/Miller Creek Duke Energy Corporation (04)

Huntersville, NC 28078 Attn: Jay Perkins Sample Matrix: Water Client Sample ID: Prism Sample ID: 2040051-02 Prism Work Order: 2040051 Time Collected: 03/30/12 09:35 Time Submitted: 04/03/12 16:40 2012007682/BioReactor 1 Inf


### J12040052

General Chemistry Parameters 6.4 *SM4500-H B P2D0057 1 4/4/12 11:30 JAB HT pH Units pH BRL *SM2320 B P2D0088 1 5.0 4/5/12 12:45 JAB mg/L 0.66 Total Alkalinity BRL *SM2320 B P2D0089 1 5.0 4/5/12 12:45 JAB mg/L 0.66 Carbonate Alkalinity BRL *SM2320 B P2D0090 1 5.0 4/5/12 12:45 JAB mg/L 0.66 Bicarbonate Alkalinity

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc. 449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 4 of 9

# Laboratory Report 04/10/2012

Result Parameter Batch ID Report Limit Analysis Date/Time Method Dilution Factor Units MDL Analyst 

### 13339 Hagers Ferry Road Project No.: Project: Allen 50/50 Somerville-Wild Boar/Miller Creek Duke Energy Corporation (04)

Huntersville, NC 28078 Attn: Jay Perkins Sample Matrix: Water Client Sample ID: Prism Sample ID: 2040051-03 Prism Work Order: 2040051 Time Collected: 03/30/12 09:46 Time Submitted: 04/03/12 16:40 2012007684/BioReactor 2 Inf


### J12040052

General Chemistry Parameters 5.8 *SM4500-H B P2D0057 1 4/4/12 11:30 JAB HT pH Units pH 15 *SM2320 B P2D0088 1 5.0 4/5/12 12:45 JAB mg/L 0.66 Total Alkalinity BRL *SM2320 B P2D0089 1 5.0 4/5/12 12:45 JAB mg/L 0.66 Carbonate Alkalinity 15 *SM2320 B P2D0090 1 5.0 4/5/12 12:45 JAB mg/L 0.66 Bicarbonate Alkalinity

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc. 449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 5 of 9

# Laboratory Report 04/10/2012

Result Parameter Batch ID Report Limit Analysis Date/Time Method Dilution Factor Units MDL Analyst 

### 13339 Hagers Ferry Road Project No.: Project: Allen 50/50 Somerville-Wild Boar/Miller Creek Duke Energy Corporation (04)

Huntersville, NC 28078 Attn: Jay Perkins Sample Matrix: Water Client Sample ID: Prism Sample ID: 2040051-04 Prism Work Order: 2040051 Time Collected: 03/30/12 09:42 Time Submitted: 04/03/12 16:40 2012007686/BioReactor 2 Eff


### J12040052

General Chemistry Parameters 6.8 *SM4500-H B P2D0057 1 4/4/12 11:30 JAB HT pH Units pH 53 *SM2320 B P2D0088 1 5.0 4/5/12 12:45 JAB mg/L 0.66 Total Alkalinity BRL *SM2320 B P2D0089 1 5.0 4/5/12 12:45 JAB mg/L 0.66 Carbonate Alkalinity 53 *SM2320 B P2D0090 1 5.0 4/5/12 12:45 JAB mg/L 0.66 Bicarbonate Alkalinity

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc. 449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 6 of 9

### 4/10/12 

## Level II QC Report

13339 Hagers Ferry Road Project No: J12040052 Project: Allen 50/50 Somerville-Wild Boar/Miller Creek Duke Energy Corporation (04)
Huntersville, NC 28078 Attn: Jay Perkins Prism Work Order: 2040051 Time Submitted: 4/3/2012 4:40:00PM

Result Limit

Reporting Units Level Spike Result

Source %REC %REC Limits RPD RPD Limit Notes Analyte General Chemistry Parameters - Quality Control



### Batch P2D0057 - NO PREP LCS (P2D0057-BS1) 

### Prepared & Analyzed: 04/04/12 pH pH Units 6.85 6.860 99-101 100 LCS (P2D0057-BS2) 

### Prepared: 04/04/12 Analyzed: 04/09/12 pH pH Units 0.00 6.860 99-101 

### Batch P2D0088 - NO PREP Blank (P2D0088-BLK1) 

### Prepared & Analyzed: 04/05/12 Total Alkalinity mg/L BRL 5.0 LCS (P2D0088-BS1) 

### Prepared & Analyzed: 04/05/12 Total Alkalinity mg/L 251 5.0 250.0 90-110 101 LCS Dup (P2D0088-BSD1) 

### Prepared & Analyzed: 04/05/12 Total Alkalinity mg/L 246 5.0 250.0 200 90-110 99 2 

### Batch P2D0089 - NO PREP Blank (P2D0089-BLK1) 

### Prepared & Analyzed: 04/05/12 Carbonate Alkalinity mg/L BRL 5.0 LCS (P2D0089-BS1) 

### Prepared & Analyzed: 04/05/12 Carbonate Alkalinity mg/L 251 5.0 90-110 LCS Dup (P2D0089-BSD1) 

### Prepared & Analyzed: 04/05/12 Carbonate Alkalinity mg/L 246 5.0 200 90-110 2

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 7 of 9

### 4/10/12 

## Level II QC Report

13339 Hagers Ferry Road Project No: J12040052 Project: Allen 50/50 Somerville-Wild Boar/Miller Creek Duke Energy Corporation (04)
Huntersville, NC 28078 Attn: Jay Perkins Prism Work Order: 2040051 Time Submitted: 4/3/2012 4:40:00PM

Result Limit

Reporting Units Level Spike Result

Source %REC %REC Limits RPD RPD Limit Notes Analyte General Chemistry Parameters - Quality Control



### Batch P2D0090 - NO PREP Blank (P2D0090-BLK1) 

### Prepared & Analyzed: 04/05/12 Bicarbonate Alkalinity mg/L BRL 5.0 LCS (P2D0090-BS1) 

### Prepared & Analyzed: 04/05/12 Bicarbonate Alkalinity mg/L 251 5.0 250.0 90-110 101 LCS Dup (P2D0090-BSD1) 

### Prepared & Analyzed: 04/05/12 Bicarbonate Alkalinity mg/L 246 5.0 250.0 200 90-110 99 2

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543 Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 

### Page 8 of 9

### Page 9 of 9

## April 10, 2012

LIMS USE: FR - TERRY WHITNER LIMS OBJECT ID: 92115655



## 92115655 Project: Pace Project No.: RE: Terry Whitner Duke Energy Carolinas, LLC PO Box 37929 DPEHS Charlotte, NC 28237

J12040052

Dear Terry Whitner: Enclosed are the analytical results for sample(s) received by the laboratory on April 03, 2012. The results relate only to the samples included in this report. Results reported herein conform to the most current TNI standards and the laboratory's Quality Assurance Manual, where applicable, unless otherwise noted in the body of the report.
Analyses were performed at the Pace Analytical Services location indicated on the sample analyte page for analysis unless otherwise footnoted. If you have any questions concerning this report, please feel free to contact me.
Sincerely,

Kevin Herring kevin.herring@pacelabs.com Project Manager

Enclosures



### REPORT OF LABORATORY ANALYSIS This report shall not be reproduced, except in full, without the written consent of Pace Analytical Services, Inc.. Page 1 of 8 Pace Analytical Services, Inc. 9800 Kincey Ave. Suite 100 Huntersville, NC 28078 (704)875-9092 Pace Analytical Services, Inc.

2225 Riverside Dr. Asheville, NC 28804 (828)254-7176 Pace Analytical Services, Inc. 205 East Meadow Road - Suite A

Eden, NC 27288 (336)623-8921

### CERTIFICATIONS

Pace Project No.: Project: 92115655

J12040052

Asheville Certification IDs 2225 Riverside Dr., Asheville, NC 28804 Florida/NELAP Certification #: E87648 Massachusetts Certification #: M-NC030 North Carolina Drinking Water Certification #: 37712 North Carolina Wastewater Certification #: 40 South Carolina Certification #: 99030001 Virginia Certification #: 00072 West Virginia Certification #: 356 Virgina/VELAP Certification #: 460147



### REPORT OF LABORATORY ANALYSIS This report shall not be reproduced, except in full, without the written consent of Pace Analytical Services, Inc.. Page 2 of 8 Pace Analytical Services, Inc. 9800 Kincey Ave. Suite 100 Huntersville, NC 28078 (704)875-9092 Pace Analytical Services, Inc.

2225 Riverside Dr. Asheville, NC 28804 (828)254-7176 Pace Analytical Services, Inc. 205 East Meadow Road - Suite A

Eden, NC 27288 (336)623-8921

### SAMPLE ANALYTE COUNT

Pace Project No.: Project: 92115655

J12040052

Lab ID Sample ID Method Analytes Reported Laboratory Analysts 92115655001 2012007680 SM 2540C 1 PASI-A LMD SM 2540D 1 PASI-A LMD



### REPORT OF LABORATORY ANALYSIS This report shall not be reproduced, except in full, without the written consent of Pace Analytical Services, Inc.. Page 3 of 8 Pace Analytical Services, Inc. 9800 Kincey Ave. Suite 100 Huntersville, NC 28078 (704)875-9092 Pace Analytical Services, Inc.

2225 Riverside Dr. Asheville, NC 28804 (828)254-7176 Pace Analytical Services, Inc. 205 East Meadow Road - Suite A

Eden, NC 27288 (336)623-8921

### ANALYTICAL RESULTS

Pace Project No.: Project: 92115655

J12040052
Sample: 2012007680 Lab ID: 92115655001 Collected: 03/30/12 09:30 Received: 04/03/12 15:03 Matrix: Water

Parameters Results Units DF Prepared Analyzed CAS No. Qual Report Limit 2540C Total Dissolved Solids Analytical Method: SM 2540C Total Dissolved Solids 10400 mg/L 1 04/04/12 17:39 500 2540D Total Suspended Solids Analytical Method: SM 2540D Total Suspended Solids 2700 mg/L 1 04/05/12 09:54 125



### REPORT OF LABORATORY ANALYSIS This report shall not be reproduced, except in full, without the written consent of Pace Analytical Services, Inc.. Date: 04/10/2012 09:21 AM Page 4 of 8 Pace Analytical Services, Inc. 9800 Kincey Ave. Suite 100 Huntersville, NC 28078 (704)875-9092 Pace Analytical Services, Inc.

2225 Riverside Dr. Asheville, NC 28804 (828)254-7176 Pace Analytical Services, Inc. 205 East Meadow Road - Suite A

Eden, NC 27288 (336)623-8921

### QUALITY CONTROL DATA

Pace Project No.: Project: 92115655

J12040052

QC Batch: QC Batch Method: Analysis Method: Analysis Description: WET/20329 SM 2540C SM 2540C 2540C Total Dissolved Solids Associated Lab Samples: 92115655001

Parameter Units Blank Result Reporting

Limit Qualifiers METHOD BLANK: 746355 Associated Lab Samples: 92115655001 Matrix: Water

Analyzed Total Dissolved Solids mg/L ND 25.0 04/04/12 17:36

Parameter Units LCS Result % Rec Limits Qualifiers % Rec Conc. 746356 LABORATORY CONTROL SAMPLE: LCS Spike
Total Dissolved Solids mg/L 204 D6 250 82 80-120

Parameter Units Dup Result Qualifiers

RPD Result 92115535003 746357 SAMPLE DUPLICATE:

Total Dissolved Solids mg/L 47.0 16 40.0



### REPORT OF LABORATORY ANALYSIS This report shall not be reproduced, except in full, without the written consent of Pace Analytical Services, Inc.. Date: 04/10/2012 09:21 AM Page 5 of 8 Pace Analytical Services, Inc. 9800 Kincey Ave. Suite 100 Huntersville, NC 28078 (704)875-9092 Pace Analytical Services, Inc.

2225 Riverside Dr. Asheville, NC 28804 (828)254-7176 Pace Analytical Services, Inc. 205 East Meadow Road - Suite A

Eden, NC 27288 (336)623-8921

### QUALITY CONTROL DATA

Pace Project No.: Project: 92115655

J12040052

QC Batch: QC Batch Method: Analysis Method: Analysis Description: WET/20340 SM 2540D SM 2540D 2540D Total Suspended Solids Associated Lab Samples: 92115655001

Parameter Units Blank Result Reporting

Limit Qualifiers METHOD BLANK: 746637 Associated Lab Samples: 92115655001 Matrix: Water

Analyzed Total Suspended Solids mg/L ND 2.5 04/05/12 09:45

Parameter Units LCS Result % Rec Limits Qualifiers % Rec Conc. 746638 LABORATORY CONTROL SAMPLE: LCS Spike Total Suspended Solids mg/L 238 250 95 80-120

Parameter Units Dup Result Qualifiers

RPD Result 92115370001 746639 SAMPLE DUPLICATE:

Total Suspended Solids mg/L 370 4 386

Parameter Units Dup Result Qualifiers

RPD Result 92115456001 746640 SAMPLE DUPLICATE:

Total Suspended Solids mg/L 8.2 D6 22 10.2



### REPORT OF LABORATORY ANALYSIS This report shall not be reproduced, except in full, without the written consent of Pace Analytical Services, Inc.. Date: 04/10/2012 09:21 AM Page 6 of 8 Pace Analytical Services, Inc. 9800 Kincey Ave. Suite 100 Huntersville, NC 28078 (704)875-9092 Pace Analytical Services, Inc.

2225 Riverside Dr. Asheville, NC 28804 (828)254-7176 Pace Analytical Services, Inc. 205 East Meadow Road - Suite A

Eden, NC 27288 (336)623-8921

### QUALIFIERS

Pace Project No.: Project: 92115655

J12040052

DEFINITIONS DF - Dilution Factor, if reported, represents the factor applied to the reported data due to changes in sample preparation, dilution of the sample aliquot, or moisture content. ND - Not Detected at or above adjusted reporting limit. J - Estimated concentration above the adjusted method detection limit and below the adjusted reporting limit. MDL - Adjusted Method Detection Limit. S - Surrogate 1,2-Diphenylhydrazine (8270 listed analyte) decomposes to Azobenzene. Consistent with EPA guidelines, unrounded data are displayed and have been used to calculate % recovery and RPD values. LCS(D) - Laboratory Control Sample (Duplicate) MS(D) - Matrix Spike (Duplicate) DUP - Sample Duplicate RPD - Relative Percent Difference NC - Not Calculable. SG - Silica Gel - Clean-Up U - Indicates the compound was analyzed for, but not detected. N-Nitrosodiphenylamine decomposes and cannot be separated from Diphenylamine using Method 8270. The result reported for each analyte is a combined concentration. Acid preservation may not be appropriate for 2-Chloroethylvinyl ether, Styrene, and Vinyl chloride. Pace Analytical is TNI accredited. Contact your Pace PM for the current list of accredited analytes. TNI - The NELAC Institute. LABORATORIES Pace Analytical Services - Asheville PASI-A ANALYTE QUALIFIERS

The relative percent difference (RPD) between the sample and sample duplicate exceeded laboratory control limits. D6



### REPORT OF LABORATORY ANALYSIS This report shall not be reproduced, except in full, without the written consent of Pace Analytical Services, Inc.. Date: 04/10/2012 09:21 AM Page 7 of 8 Pace Analytical Services, Inc. 9800 Kincey Ave. Suite 100 Huntersville, NC 28078 (704)875-9092 Pace Analytical Services, Inc.

2225 Riverside Dr. Asheville, NC 28804 (828)254-7176 Pace Analytical Services, Inc. 205 East Meadow Road - Suite A

Eden, NC 27288 (336)623-8921

### QUALITY CONTROL DATA CROSS REFERENCE TABLE

Pace Project No.: Project: 92115655

J12040052

Lab ID Sample ID QC Batch Method QC Batch Analytical Method Analytical Batch 92115655001 WET/20329 2012007680 SM 2540C 92115655001 WET/20340 2012007680 SM 2540D



### REPORT OF LABORATORY ANALYSIS This report shall not be reproduced, except in full, without the written consent of Pace Analytical Services, Inc.. Date: 04/10/2012 09:21 AM Page 8 of 8 Pace Analytical Services, Inc. 9800 Kincey Ave. Suite 100 Huntersville, NC 28078 (704)875-9092 Pace Analytical Services, Inc.

2225 Riverside Dr. Asheville, NC 28804 (828)254-7176 Pace Analytical Services, Inc. 205 East Meadow Road - Suite A

Eden, NC 27288 (336)623-8921

### 3958 6th

### Ave NW • Seattle, WA 98107 • T: 206-632-6206 • F: 206-632-6017 • www.brooksrand.com • brl@brooksrand.com 

## April 11, 2012
Duke Energy ATTN: Jay Perkins Scientific Support-Laboratory 13339 Hagers Ferry Road Huntersville NC 28078 jcperkins@duke-energy.com labcustomer@duke-energy.com
RE: Project DUK-HV1201 Client Project: J12040052 Dear Mr. Perkins, On April 4, 2012, Brooks Rand Labs (BRL) received three (3) wastewater samples and three (3) corresponding field blanks. Samples were logged-in for total mercury (THg) and dissolved mercury (DHg). All samples were received, prepared, analyzed, and stored according to BRL SOPs and EPA methodology. The results were blank-corrected as described in the calculations section of the applicable SOP(s) and may be evaluated using adjusted reporting limits to account for sample aliquot size. Please refer to the Sample Results page for sample-specific detection limits and other details.Data used for regulatory purposes has a 24 hour filtration holding time requirement. Nonregulatory purposed data has a 48 hour filtration holding time. The samples were received outside of the 48 hours of filtration requirement and dissolved Hg sample results were qualified Has estimates. Aside from concentration qualifiers, all data was reported without further qualification and all associated quality control sample results met the acceptance criteria. BRL, an accredited laboratory, certifies the reported results of all analyses for which BRL is NELAP accredited meet all NELAP requirements. For more details, see the Report Information page of the report. Please feel free to contact me if you have any questions regarding this report. Sincerely,

Lydia Greaves Project Manager lydia@brooksrand.com

### Project ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



## Definition of Data Qualifiers

(Effective 9/23/09) 

## Laboratory Accreditation BRL is accredited by the National Environmental Laboratory Accreditation Program (NELAP) through the State of Florida Department of Health, Bureau of Laboratories (E87982) and is certified to perform many environmental analyses. BRL is also certified by many other states to perform environmental analyses. For a current list of our accreditations /certifications, please visit our website at <http://www.brooksrand.com/default.asp?contentID=586>. Results reported relate only to the samples listed in the report. 

# Report Information

BLK BRL BS CAL CCV CRM D DUP ICV MSD ND NR PS REC RPD RSD SCV SOP 

### method blank Brooks Rand Labs laboratory fortified blank calibration standard continuing calibration verification certified reference material dissolved fraction duplicate initial calibration verification matrix spike duplicate non-detect non-reportable post preparation spike percent recovery relative percent difference relative standard deviation secondary calibration verification standard operating procedure MDL MRL MS



### method detection limit method reporting limit matrix spike

SRM T COC



### standard reference material total recoverable fraction chain of custody record 

## Common Abbreviations

These qualifiers are based on those previously utilized by Brooks Rand, Ltd., those found in the EPA SOW ILM03.0, Exhibit B, Section III, pg. B-18, and the USEPA Laboratory Data Validation Functional Guidelines for Evaluating Inorganic Analyses; USEPA; July 2002. These supersede all previous qualifiers ever employed by BRL. Detected by the instrument, the result is > the MDL but ≤the MRL. Result is reported and considered an estimate. B E 

### An estimated value due to the presence of interferences. A full explanation is presented in the narrative. H 

### Holding time and/or preservation requirements not met. Result is estimated. J 

### Estimated value. A full explanation is presented in the narrative. J-M 

### Duplicate precision (RPD) for associated QC sample was not within acceptance criteria. Result is estimated. J-N 

### Spike recovery for associated QC sample was not within acceptance criteria. Result is estimated. M 

### Duplicate precision (RPD) was not within acceptance criteria. Result is estimated. N 

### Spike recovery was not within acceptance criteria. Result is estimated. R 

### Rejected, unusable value. A full explanation is presented in the narrative. U 

### Result is ≤the MDL or client requested reporting limit (CRRL). Result reported as the MDL or CRRL. X 

### Result is not BLK-corrected and is within 10x the absolute value of the highest detectable BLK in the batch. Result is estimated. 

## Field Quality Control Samples Please be notified that certain EPA methods require the collection of field quality control samples of an appropriate type and frequency; failure to do so is considered a deviation from some methods and for compliance purposes should only be done with the approval of regulatory authorities. Please see the specific EPA methods for details regarding required field quality control samples.

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

### Project ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Sample Information

Report Matrix Type Received Sampled Sample Lab ID 

### 1214024-01 BioReactor 1 Inf 03/30/2012 04/04/2012 Sample FGD Wastewater 1214024-02 BioReactor 1 Inf 03/30/2012 04/04/2012 Sample FGD Wastewater 1214024-03 BioReactor 1 Inf Hg Blk 03/30/2012 04/04/2012 Field Blank DIW 1214024-04 BioReactor 1 Inf Hg Blk 03/30/2012 04/04/2012 Field Blank DIW 1214024-05 BioReactor 2 Inf 03/30/2012 04/04/2012 Sample FGD Wastwater 1214024-06 BioReactor 2 Inf 03/30/2012 04/04/2012 Sample FGD Wastwater 1214024-07 BioReactor 2 Inf Hg Blk 03/30/2012 04/04/2012 Field Blank DIW 1214024-08 BioReactor 2 Inf Hg Blk 03/30/2012 04/04/2012 Field Blank DIW 1214024-09 BioReactor 2 Eff 03/30/2012 04/04/2012 Sample FGD Wastwater 1214024-10 BioReactor 2 Eff 03/30/2012 04/04/2012 Sample FGD Wastwater 1214024-11 BioReactor 2 Eff Hg Blk 03/30/2012 04/04/2012 Field Blank DIW 1214024-12 BioReactor 2 Eff Hg Blk 03/30/2012 04/04/2012 Field Blank DIW 

# Batch Summary

Analyte Prepared Analyzed Sequence Batch Lab Matrix Method 

### B120579 04/06/2012 04/11/2012 1200255 Water EPA 1631 Hg

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

### Project ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Sample Results

Sample Sequence Result MDL MRL Unit Batch

Qualifier Analyte Fraction

Report Matrix 

### BioReactor 1 Inf 

### ng/L 107 T 1200255 B120579 FGD Wastewater 40.4 15.2 1214024-01 Hg ng/L 41.1 D H 1200255 B120579 FGD Wastewater 4.08 1.53 1214024-02 Hg
BioReactor 1 Inf Hg Blk 

### ng/L 0.15 T U 1200255 B120579 DIW 0.40 0.15 1214024-03 Hg ng/L 0.15 D H, U 1200255 B120579 DIW 0.40 0.15 1214024-04 Hg

BioReactor 2 Eff 

### ng/L 45.5 T 1200255 B120579 FGD Wastwater 2.06 0.77 1214024-09 Hg ng/L 9.94 D H 1200255 B120579 FGD Wastwater 1.00 0.38 1214024-10 Hg
BioReactor 2 Eff Hg Blk 

### ng/L 0.15 T U 1200255 B120579 DIW 0.41 0.15 1214024-11 Hg ng/L 0.15 D H, U 1200255 B120579 DIW 0.40 0.15 1214024-12 Hg

BioReactor 2 Inf 

### ng/L 157 T 1200255 B120579 FGD Wastwater 8.16 3.06 1214024-05 Hg ng/L 15.5 D H 1200255 B120579 FGD Wastwater 4.08 1.53 1214024-06 Hg
BioReactor 2 Inf Hg Blk 

### ng/L 0.15 T U 1200255 B120579 DIW 0.41 0.15 1214024-07 Hg ng/L 0.15 D H, U 1200255 B120579 DIW 0.41 0.15 1214024-08 Hg

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

### Project ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Accuracy & Precision Summary

Batch: 

### B120579

Analyte Result Units Native Spike REC & Limits RPD & Limits Method: 

### EPA 1631 Lab Matrix: 

### Water

Sample Certified Reference Material (1213013, NIST 1641d 1000x dilution) B120579-SRM1 

### 15.68 104% ng/L 85-115 Hg 16.38
Matrix Spike (1214024-01) B120579-MS1 

### 555.6 108% ng/L 71-125 Hg 706.4

107.5
Matrix Spike Duplicate (1214024-01) B120579-MSD1 

### 555.6 112% ng/L 71-125 243% Hg 728.5

107.5
Matrix Spike (1214024-09) B120579-MS2 

### 219.5 109% ng/L 71-125 Hg 285.1

45.52
Matrix Spike Duplicate (1214024-09) B120579-MSD2 

### 229.1 110% ng/L 71-125 244% Hg 297.4

45.52

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

### Project ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Method Blanks & Reporting Limits

Batch: 

### B120579
Method: 

### EPA 1631 Matrix: 

### Water Analyte: 

### Hg Result Units Sample 

### B120579-BLK1 ng/L 0.09 B120579-BLK2 ng/L 0.09 B120579-BLK3 ng/L 0.06 B120579-BLK4 ng/L 0.10 MDL: 

### 0.15 Average: 

### 0.09 Standard Deviation: 

### 0.02 Limit: 

### 0.10 Limit: 

### 0.50 MRL: 

### 0.40

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

### Project ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Instrument Calibration

Sequence: 

### 1200255 Method: 

### EPA 1631 Instrument: 

### THG-10 Date: 

### 04/11/2012 Total Mercury and Mercury Speciation by CVAFS
Analyte: 

### Hg REC & Limits Result True Value Lab ID Units 

### 1200255-IBL1 3.95 pg of Hg 1200255-IBL2 3.10 pg of Hg 1200255-IBL3 3.64 pg of Hg 1200255-IBL4 4.89 pg of Hg 1200255-CAL1 25.00 24.14 pg of Hg 97% 1200255-CAL2 100.0 99.11 pg of Hg 99% 1200255-CAL3 500.0 489.7 pg of Hg 98% 1200255-CAL4 2500 2598 pg of Hg 104% 1200255-CAL5 10000 10290 pg of Hg 103% 1200255-ICV1 1568 1638 pg of Hg 104% 85-115 1200255-CCV1 500.0 515.1 pg of Hg 103% 77-123 1200255-CCB1 6.49 pg of Hg 1200255-CCV2 500.0 506.4 pg of Hg 101% 77-123 1200255-CCV3 500.0 521.1 pg of Hg 104% 77-123

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

### Project ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Sample Containers

Lab ID: 

### 1214024-01 Report Matrix: 

### FGD Wastewater Sample Type: 

### Sample Received: 

### 04/04/2012 Sample: 

### BioReactor 1 Inf Collected: 

### 03/30/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71536910 10 Cooler

None N/A 250 mL Bottle FLPE Hg-T

Lab ID: 

### 1214024-02 Report Matrix: 

### FGD Wastewater Sample Type: 

### Sample Received: 

### 04/04/2012 Sample: 

### BioReactor 1 Inf Collected: 

### 03/30/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71536910 10 Cooler

None N/A 250 mL Bottle FLPE Hg-T

Lab ID: 

### 1214024-03 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 04/04/2012 Sample: 

### BioReactor 1 Inf Hg Blk Collected: 

### 03/30/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71536910 10 Cooler

None N/A 250 mL Bottle FLPE Hg-T

Lab ID: 

### 1214024-04 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 04/04/2012 Sample: 

### BioReactor 1 Inf Hg Blk Collected: 

### 03/30/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71536910 10 Cooler

None N/A 250 mL Bottle FLPE Hg-T

Lab ID: 

### 1214024-05 Report Matrix: 

### FGD Wastwater Sample Type: 

### Sample Received: 

### 04/04/2012 Sample: 

### BioReactor 2 Inf Collected: 

### 03/30/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71536910 10 Cooler

None N/A 250 mL Bottle FLPE Hg-T

Lab ID: 

### 1214024-06 Report Matrix: 

### FGD Wastwater Sample Type: 

### Sample Received: 

### 04/04/2012 Sample: 

### BioReactor 2 Inf Collected: 

### 03/30/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71536910 10 Cooler

None N/A 250 mL Bottle FLPE Hg-T

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

### Project ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Sample Containers

Lab ID: 

### 1214024-07 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 04/04/2012 Sample: 

### BioReactor 2 Inf Hg Blk Collected: 

### 03/30/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71536910 10 Cooler

None N/A 250 mL Bottle FLPE Hg-T

Lab ID: 

### 1214024-08 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 04/04/2012 Sample: 

### BioReactor 2 Inf Hg Blk Collected: 

### 03/30/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71536910 10 Cooler

None N/A 250 mL Bottle FLPE Hg-T

Lab ID: 

### 1214024-09 Report Matrix: 

### FGD Wastwater Sample Type: 

### Sample Received: 

### 04/04/2012 Sample: 

### BioReactor 2 Eff Collected: 

### 03/30/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71536910 10 Cooler

None N/A 250 mL Bottle FLPE Hg-T

Lab ID: 

### 1214024-10 Report Matrix: 

### FGD Wastwater Sample Type: 

### Sample Received: 

### 04/04/2012 Sample: 

### BioReactor 2 Eff Collected: 

### 03/30/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71536910 10 Cooler

None N/A 250 mL Bottle FLPE Hg-T

Lab ID: 

### 1214024-11 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 04/04/2012 Sample: 

### BioReactor 2 Eff Hg Blk Collected: 

### 03/30/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71536910 10 Cooler

None N/A 250 mL Bottle FLPE Hg-T

Lab ID: 

### 1214024-12 Report Matrix: 

### DIW Sample Type: 

### Field Blank Received: 

### 04/04/2012 Sample: 

### BioReactor 2 Eff Hg Blk Collected: 

### 03/30/2012 Ship. Cont. pH P-Lot Preservation Lot Size Container Des 

### A 71536910 10 Cooler

None N/A 250 mL Bottle FLPE Hg-T

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

### Project ID: 

### DUK-HV1201

PM: 

### Tiffany Stilwater Client PM: 

### Jay Perkins Client PO: 

### 141391



# Shipping Containers

Cooler Tracking No: 

### 472679669812 via FedEx Temperature: 

### 1.8 °C Coolant Type: 

### Ice Description: 

### Cooler Damaged in transit? 

### No Returned to client? 

### No Custody seals present? 

### No Custody seals intact? 

### No COC present? 

### Yes Received: 

### April 4, 2012 13:33

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

## April 11 , 201 2

Jay Perkins Duke Energy Analytical Laboratory Mail Code MGO3A2 (Building 7405) 13339 Hagers Ferry Rd. Huntersville, NC 28078 (704) 875-5245 Project: Allen 50/50 Somerville-Wild Boar/Miller Creek (MACT/CERT) (LIMS # J1 2040052)

Dear Mr. Perkins, Attached is the report associated with four (4) aqueous samples submitted for selenium speciation analysis on April 3 , 2012

.The samples were received in a sealed cooler at-0. 6°C on April 4, 2012

.Selenium speciation analysis was performed via ion chromatography inductively coupled plasma dynamic reaction cell mass spectrometry (IC-ICP-DRC-MS). Any issues associated with the analysis are addressed in the following report. If you have any questions, please feel free to contact me at your convenience.
Sincerely,

Russell Gerads Vice President Applied Speciation and Consulting, LLC

## Applied Speciation and Consulting, LLC Report prepared for: Jay Perkins Duke Energy Analytical Laboratory Mail Code MGO3A2 (Building 7405) 13339 Hagers Ferry Rd. Huntersville, NC 28078 Project: Allen 50/50 Somerville-Wild Boar/Miller Creek (MACT/CERT) (LIMS # J12040052)
April 11 , 2012

1. Sample Reception Four (4) aqueous samples in125mL HDPE bottles (provided by Applied Speciation and Consulting) were submitted for selenium speciation analysis on April 3 , 2012. The samples were received on April 4, 2012 in a sealed container at -0.6°C. The samples were received in a laminar flow clean hood,void of trace metals contamination and ultra-violet radiation, and was designated a discrete sample identifier. An aliquot of each sample was filtered (0.45μm) and each filtrate wasstored in a secure, monitored cryofreezer (maintained at a temperature of -80o

## C) until selenium speciation analysis could be performed via ion chromatography inductively coupled plasma dynamic reaction cell mass spectrometry (IC-ICP-DRC-MS).

2. Sample Preparation All sample preparation is performed in laminar flow clean hoods known to be free from trace metals contamination. All applied water for dilutions and sample preservatives are monitored for contamination to account for any biases associated with the sample results. Selenium Speciation Analysis by IC-ICP-DRC

-MS Prior to analysis, an aliquot of each sample was filtered with a syringe filter (0.45μm) and injected directly into a sealed autosampler vial. No further sample preparation was performed as any chemical alteration of a sample may shift the equilibrium of the system, resulting in changes in speciation ratios. 3. Sample Analysis All sample analysis is preceded by a minimum of a five-point calibration curve spanning the entire concentration range of interest. Calibration curves are performed at the beginning of each analytical day. All calibration curves, associated with each species of interest, are

## standardized by linear regression resulting in a response factor. All sample results are

instrument blank correctedto account for any operational biases associated with the analytical platform. Prior to sample analysis, all calibration curves are verified using second source standards which are identified as initial calibration verification standards (ICV). Ongoing instrument performance is identified by the analysis of continuing calibration verification standards (CCV) and continuing calibration blanks (CCB) at a minimal interval of every ten analytical runs. Selenium Speciation Analysis by IC-ICP-DRC

-MS Eachsample for selenium speciation analysis wasanalyzed by ion chromatography inductively coupled plasma dynamic reaction cell mass spectrometry (IC-ICP-DRC-MS) on April 4, 2012. An aliquot of eachsample is injected onto an anion exchange column and mobilized by a basic (pH > 7) gradient. The eluting selenium species are then introduced into a radio frequency (RF) plasma where energy-transfer processes cause desolvation, atomization, and ionization. The ions are extracted from the plasma through a differentially-pumped vacuum interface and travel through a pressurized chamber (DRC) containing a reaction gas which preferentially reacts with interfering ions of the same target mass to charge ratios (m/z). A solid-state detector detects ions transmitted through the mass analyzer and the resulting current is processed by a data handling system. Retention times for each eluting species are compared to known standards for species identification.

4. Analytical Issues The overall analyses went well and no significant analytical issues were encountered. All quality control parameters associated with this sample were within acceptance limits. The estimated method detection limits (eMDLs) for selenite, selenate, and selenocyanate are generated from replicate analyses of the lowest standard in the calibration curve. Not all selenium species are present in preparation blanks; therefore, eMDL calculations based on preparation blanks are artificially biased low. The eMDL for methylseleninic acid and selenomethionine is calculated from the average eMDL of selenite, selenate, and selenocyanate. The calibration does not contain methylseleninic acid or selenomethionine due to impurities in these standards which would bias the results for other selenium species.

## If you have any questions or concerns regarding this report, please feel free to contact me.

Sincerely,

Russell Gerads Vice President Applied Speciation and Consulting, LLC

### Sample Results

Sample ID Se(IV) Se(VI) SeCN MeSe(IV) SeMe Unknown Se Species (n) FGD Purge Eff 226 81.5 4.7 44.5 ND (<1.2) 0 (0) BioReactor 1 Inf 68.9 21.9 ND (<0.31) 23.4 ND (<0.31) 10.8 (1) BioReactor 2 Inf 4.34 ND (<0.33) 0.36 0.73 ND (<0.31) 0.69 (1) BioReactor 2 Eff 0.60 ND (<0.33) ND (<0.31) ND (<0.31) ND (<0.31) 0 (0) All results reflect the applied dilution and are reported in μg/L ND = Not detected at the applied dilution SeCN = Selenocyanate MeSe(IV) = Methylseleninic acid SeMe = Selenomethionine Unknown Se Species = Total concentration of all unknown Se species observed by IC-ICP-MS n = number of unknown Se species observed Report Generated by: Russell Gerads Applied Speciation and Consulting, LLC Selenium Speciation Results for Duke Energy Project Name: Allen 50/50 Somerville-wild Boar/Miller Creek (MACT/CERT) Contact: Jay Perkins
Date: April 11, 2012 LIMS #J12040052

### Quality Control Summary - Preparation Blank Summary Analyte (μg/L) PBW1 PBW2 PBW3 PBW4 Mean StdDev eMDL* eMDL 50x eMDL 200x Se(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.006 0.29 1.2 Se(VI) 0.000 0.000 0.000 0.000 0.000 0.000 0.007 0.33 1.3 SeCN 0.000 0.000 0.000 0.000 0.000 0.000 0.006 0.31 1.2 MeSe(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.006 0.31 1.2 SeMe 0.000 0.000 0.000 0.000 0.000 0.000 0.006 0.31 1.2 eMDL = Estimated Method Detection Limit *Please see narrative regarding eMDL calculations
Quality Control Summary - Certified Reference Materials Analyte (μg/L) CRM True Value Result Recovery Se(IV) LCS 9.57 9.58 100.2 Se(VI) LCS 9.48 9.53 100.5 SeCN LCS 8.92 9.14 102.5 MeSe(IV) LCS 6.47 6.49 100.3 SeMe LCS 9.32 9.19 98.6 Applied Speciation and Consulting, LLC Selenium Speciation Results for Duke Energy Project Name: Allen 50/50 Somerville-wild Boar/Miller Creek (MACT/CERT) Contact: Jay Perkins LIMS #J12040052 Date: April 11, 2012 Report Generated by: Russell Gerads

### Quality Control Summary - Matrix Duplicates Analyte (μg/L) Sample ID Rep 1 Rep 2 Mean RPD Se(IV) Batch QC 7.56 7.13 7.34 5.7 Se(VI) Batch QC ND (<0.33) ND (<0.33) NC NC SeCN Batch QC 2.08 2.49 2.29 17.8 MeSe(IV) Batch QC ND (<0.31) ND (<0.31) NC NC SeMe Batch QC ND (<0.31) ND (<0.31) NC NC ND = Not detected at the applied dilution NC = Value was not calculated due to one or more concentrations below the eMDL

Quality Control Summary - Matrix Spike/ Matrix Spike Duplicate Analyte (μg/L) Sample ID Spike Conc MS Result Recovery Spike Conc MSD Result Recovery RPD Se(IV) Batch QC 278.0 281.1 98.5 278.0 283.9 99.5 1.0 Se(VI) Batch QC 252.3 259.3 102.8 252.3 261.3 103.6 0.8 SeCN Batch QC 228.8 230.3 99.7 228.8 233.7 101.2 1.5 Applied Speciation and Consulting, LLC Selenium Speciation Results for Duke Energy Project Name: Allen 50/50 Somerville-wild Boar/Miller Creek (MACT/CERT) Contact: Jay Perkins LIMS #J12040052 Date: April 11, 2012 Report Generated by: Russell Gerads