13339 Hagers Ferry Road

Huntersville, NC 28078-7929

McGuire Nuclear Complex - MG03A2

Phone: 980-875-5245 Fax: 980-875-4349

Order Summary Report

Analytical Laboratory

Order Number: J13010488

Project Name: Wastewater Nietering

Lab Contact: Jason C Perkins

Date: 2/20/2013

Customer Address: 253 Plant Allen Road

Customer Name(s): Bill K., Ron L., Don S.

Belmont, NC 28012

Phone: 980-875-5348

Report Authorized By:

(Signature)

Program Comments:

Please contact the Program Manager (Jason C Perkins) with any questions regarding this report.

Data Flags & Calculations:

Any analytical tests or individual analytes within a test flagged with a Qualifier indicate a deviation from the method quality 

system or quality control requirement. The qualifier description is found at the end of the Certificate of Analysis (sample results) 

under the qualifiers heading. All results are reported on a dry weight basis unless otherwise noted. Subcontracted data 

included on the Duke Certificate of Analysis is to be used as information only. Certified vendor results can be found in the 

subcontracted lab final report. Duke Energy Analytical Laboratory subcontracts analyses to other vendor laboratories that have 

been qualified by Duke Energy to perform these analyses except where noted.

Data Package:

This data package includes analytical results that are applicable only to the samples described in this narrative. An estimation of 

the uncertainty of measurement for the results in the report is available upon request. This report shall not be reproduced, except 

in full, without the written consent of the Analytical Laboratory. Please contact the Analytical laboratory with any questions. The 

order of individual sections within this report is as follows:

Job Summary Report, Sample Identification, Technical Validation of Data Package, Analytical Laboratory Certificate of Analysis, 

Analytical Laboratory QC Reports, Sub-contracted Laboratory Results, Customer Specific Data Sheets, Reports & 

Documentation, Customer Database Entries, Test Case Narratives, Chain of Custody (COC)

Certification:

The Analytical Laboratory holds the following State Certifications : North Carolina (DENR) Certificate #248, South Carolina 

(DHEC) Laboratory ID # 99005. Contact the Analytical Laboratory for definitive information about the certification status of 

specific methods. 

Jason C Perkins

Page 1 of 37
Sample ID's & Descriptions:

Sample ID Plant/Station

Collection 

Date and Time Collected By Sample Description

2013002243 ALLEN 25-Jan-13 8:20 AM BILL HASKINS FGD Purge Eff 

2013002244 ALLEN 25-Jan-13 8:25 AM BILL HASKINS EQ Tank Eff 

2013002245 ALLEN 25-Jan-13 8:23 AM BILL HASKINS BioReactor 1 Inf 

2013002246 ALLEN 25-Jan-13 8:23 AM BILL HASKINS BioReactor 1 Inf BLANK 

2013002247 ALLEN 25-Jan-13 8:31 AM BILL HASKINS BioReactor 2 Inf 

2013002248 ALLEN 25-Jan-13 8:31 AM BILL HASKINS BioReactor 2 Inf BLANK 

2013002249 ALLEN 25-Jan-13 8:28 AM BILL HASKINS BioReactor 2 Eff 

2013002250 ALLEN 25-Jan-13 8:28 AM BILL HASKINS BioReactor 2 Eff BLANK 

2013002251 ALLEN 25-Jan-13 10:20 AM BILL HASKINS Filter Blk 

9 Total Samples

Page 2 of 37
COC and .pdf report are in agreement with sample totals 

and analyses (compliance programs and procedures).

All Results are less than the laboratory reporting limits.

All laboratory QA/QC requirements are acceptable.

Yes No

Technical Validation Review

Checklist:

Yes No

Yes No

Report Sections Included:

Job Summary Report Sub-contracted Laboratory Results

Sample Identification Customer Specific Data Sheets, Reports, & Documentation

Technical Validation of Data Package Customer Database Entries

Analytical Laboratory Certificate of Analysis

Analytical Laboratory QC Report

Chain of Custody

Reviewed By: DBA Account Date: 2/20/2013

Electronic Data Deliverable (EDD) Sent Separately

Page 3 of 37
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J13010488

2013002243

Collection Date: 25-Jan-13 8:20 AM

Site: FGD Purge Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY - (Analysis Performed by Prism Labs)

Vendor Parameter Complete Vendor Method V_PRISM

INORGANIC IONS BY IC

Bromide 310 mg/L 5 50 EPA 300.0 02/14/2013 16:27 BGN9034

Chloride 3200 mg/L 100 1000 EPA 300.0 02/14/2013 16:27 BGN9034

Sulfate 1500 mg/L 100 1000 EPA 300.0 02/14/2013 16:27 BGN9034

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) 56.5 ug/L 2.5 50 EPA 245.1 01/31/2013 14:24 AGIBBS

DISSOLVED METALS BY ICP

Manganese (Mn) 1.30 mg/L 0.05 10 EPA 200.7 02/05/2013 12:16 MHH7131

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 246 mg/L 0.5 10 EPA 200.7 01/31/2013 09:22 MHH7131

Calcium (Ca) 2530 mg/L 0.1 10 EPA 200.7 01/31/2013 09:22 MHH7131

Iron (Fe) 160 mg/L 0.1 10 EPA 200.7 01/31/2013 09:22 MHH7131

Magnesium (Mg) 877 mg/L 0.05 10 EPA 200.7 01/31/2013 09:22 MHH7131

Manganese (Mn) 9.80 mg/L 0.05 10 EPA 200.7 01/31/2013 09:22 MHH7131

DISSOLVED METALS BY ICP-MS

Selenium (Se) 575 ug/L 10 10 EPA 200.8 02/04/2013 15:07 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) 299 ug/L 10 10 EPA 200.8 02/01/2013 15:07 KRICHAR

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 02/01/2013 15:07 KRICHAR

Chromium (Cr) 223 ug/L 10 10 EPA 200.8 02/01/2013 15:07 KRICHAR

Copper (Cu) 232 ug/L 10 10 EPA 200.8 02/01/2013 15:07 KRICHAR

Nickel (Ni) 299 ug/L 10 10 EPA 200.8 02/01/2013 15:07 KRICHAR

Selenium (Se) 2560 ug/L 10 10 EPA 200.8 02/01/2013 15:07 KRICHAR

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 02/01/2013 15:07 KRICHAR

Zinc (Zn) 509 ug/L 10 10 EPA 200.8 02/01/2013 15:07 KRICHAR

SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete Vendor Method V_AS&C

TOTAL DISSOLVED SOLIDS

TDS 12000 mg/L 200 1 SM2540C 01/30/2013 16:20 SWILLI3

TOTAL SUSPENDED SOLIDS

TSS 3900 mg/L 250 1 SM2540D 01/31/2013 14:05 SWILLI3

Page 4 of 37
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J13010488

2013002244

Collection Date: 25-Jan-13 8:25 AM

Site: EQ Tank Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY (COLD VAPOR) IN WATER

Mercury (Hg) 39.3 ug/L 2.5 50 EPA 245.1 01/31/2013 14:26 AGIBBS

DISSOLVED METALS BY ICP

Manganese (Mn) < 0.05 mg/L 0.05 10 EPA 200.7 02/05/2013 12:20 MHH7131

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 204 mg/L 0.5 10 EPA 200.7 01/31/2013 09:26 MHH7131

Calcium (Ca) 2120 mg/L 0.1 10 EPA 200.7 01/31/2013 09:26 MHH7131

Iron (Fe) 118 mg/L 0.1 10 EPA 200.7 01/31/2013 09:26 MHH7131

Magnesium (Mg) 741 mg/L 0.05 10 EPA 200.7 01/31/2013 09:26 MHH7131

Manganese (Mn) 6.58 mg/L 0.05 10 EPA 200.7 01/31/2013 09:26 MHH7131

DISSOLVED METALS BY ICP-MS

Selenium (Se) 752 ug/L 10 10 EPA 200.8 02/04/2013 15:10 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) 212 ug/L 10 10 EPA 200.8 02/01/2013 15:24 KRICHAR

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 02/01/2013 15:24 KRICHAR

Chromium (Cr) 174 ug/L 10 10 EPA 200.8 02/01/2013 15:24 KRICHAR

Copper (Cu) 178 ug/L 10 10 EPA 200.8 02/01/2013 15:24 KRICHAR

Nickel (Ni) 243 ug/L 10 10 EPA 200.8 02/01/2013 15:24 KRICHAR

Selenium (Se) 1870 ug/L 10 10 EPA 200.8 02/01/2013 15:24 KRICHAR

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 02/01/2013 15:24 KRICHAR

Zinc (Zn) 386 ug/L 10 10 EPA 200.8 02/01/2013 15:24 KRICHAR

2013002245

Collection Date: 25-Jan-13 8:23 AM

Site: BioReactor 1 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY - (Analysis Performed by Prism Labs)

Vendor Parameter Complete Vendor Method V_PRISM

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

DISSOLVED METALS BY ICP

Manganese (Mn) < 0.05 mg/L 0.05 10 EPA 200.7 02/05/2013 12:24 MHH7131

Page 5 of 37
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J13010488

2013002245

Collection Date: 25-Jan-13 8:23 AM

Site: BioReactor 1 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 109 mg/L 0.5 10 EPA 200.7 01/31/2013 09:30 MHH7131

Calcium (Ca) 1630 mg/L 0.1 10 EPA 200.7 01/31/2013 09:30 MHH7131

Iron (Fe) < 0.1 mg/L 0.1 10 EPA 200.7 01/31/2013 09:30 MHH7131

Magnesium (Mg) 353 mg/L 0.05 10 EPA 200.7 01/31/2013 09:30 MHH7131

Manganese (Mn) < 0.05 mg/L 0.05 10 EPA 200.7 01/31/2013 09:30 MHH7131

DISSOLVED METALS BY ICP-MS

Selenium (Se) 571 ug/L 10 10 EPA 200.8 02/04/2013 15:13 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 10 ug/L 10 10 EPA 200.8 02/01/2013 15:14 KRICHAR

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 02/01/2013 15:14 KRICHAR

Chromium (Cr) < 10 ug/L 10 10 EPA 200.8 02/01/2013 15:14 KRICHAR

Copper (Cu) < 10 ug/L 10 10 EPA 200.8 02/01/2013 15:14 KRICHAR

Nickel (Ni) < 10 ug/L 10 10 EPA 200.8 02/01/2013 15:14 KRICHAR

Selenium (Se) 579 ug/L 10 10 EPA 200.8 02/01/2013 15:14 KRICHAR

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 02/01/2013 15:14 KRICHAR

Zinc (Zn) < 10 ug/L 10 10 EPA 200.8 02/01/2013 15:14 KRICHAR

SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete Vendor Method V_AS&C

2013002246

Collection Date: 25-Jan-13 8:23 AM

Site: BioReactor 1 Inf BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

2013002247

Collection Date: 25-Jan-13 8:31 AM

Site: BioReactor 2 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY - (Analysis Performed by Prism Labs)

Vendor Parameter Complete Vendor Method V_PRISM

Page 6 of 37
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J13010488

2013002247

Collection Date: 25-Jan-13 8:31 AM

Site: BioReactor 2 Inf 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

DISSOLVED METALS BY ICP

Manganese (Mn) 0.102 mg/L 0.05 10 EPA 200.7 02/05/2013 12:28 MHH7131

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 85.4 mg/L 0.5 10 EPA 200.7 01/31/2013 09:34 MHH7131

Calcium (Ca) 1640 mg/L 0.1 10 EPA 200.7 01/31/2013 09:34 MHH7131

Iron (Fe) 0.229 mg/L 0.1 10 EPA 200.7 01/31/2013 09:34 MHH7131

Magnesium (Mg) 338 mg/L 0.05 10 EPA 200.7 01/31/2013 09:34 MHH7131

Manganese (Mn) 0.112 mg/L 0.05 10 EPA 200.7 01/31/2013 09:34 MHH7131

DISSOLVED METALS BY ICP-MS

Selenium (Se) 77.3 ug/L 10 10 EPA 200.8 02/04/2013 15:26 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 10 ug/L 10 10 EPA 200.8 02/01/2013 15:17 KRICHAR

Cadmium (Cd) < 10 ug/L 10 10 EPA 200.8 02/01/2013 15:17 KRICHAR

Chromium (Cr) < 10 ug/L 10 10 EPA 200.8 02/01/2013 15:17 KRICHAR

Copper (Cu) < 10 ug/L 10 10 EPA 200.8 02/01/2013 15:17 KRICHAR

Nickel (Ni) < 10 ug/L 10 10 EPA 200.8 02/01/2013 15:17 KRICHAR

Selenium (Se) 77.6 ug/L 10 10 EPA 200.8 02/01/2013 15:17 KRICHAR

Silver (Ag) < 10 ug/L 10 10 EPA 200.8 02/01/2013 15:17 KRICHAR

Zinc (Zn) < 10 ug/L 10 10 EPA 200.8 02/01/2013 15:17 KRICHAR

SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete Vendor Method V_AS&C

2013002248

Collection Date: 25-Jan-13 8:31 AM

Site: BioReactor 2 Inf BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

Page 7 of 37
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J13010488

2013002249

Collection Date: 25-Jan-13 8:28 AM

Site: BioReactor 2 Eff 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

ALKALINITY - (Analysis Performed by Prism Labs)

Vendor Parameter Complete Vendor Method V_PRISM

INORGANIC IONS BY IC

Bromide 530 mg/L 100 1000 EPA 300.0 02/14/2013 13:37 BGN9034

Chloride 2000 mg/L 100 1000 EPA 300.0 02/14/2013 13:37 BGN9034

Sulfate 1500 mg/L 100 1000 EPA 300.0 02/14/2013 13:37 BGN9034

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

DISSOLVED METALS BY ICP

Manganese (Mn) 0.157 mg/L 0.05 10 EPA 200.7 02/05/2013 12:32 MHH7131

TOTAL RECOVERABLE METALS BY ICP

Boron (B) 40.4 mg/L 0.5 10 EPA 200.7 01/31/2013 09:38 MHH7131

Calcium (Ca) 1430 mg/L 0.1 10 EPA 200.7 01/31/2013 09:38 MHH7131

Iron (Fe) 0.624 mg/L 0.1 10 EPA 200.7 01/31/2013 09:38 MHH7131

Magnesium (Mg) 263 mg/L 0.05 10 EPA 200.7 01/31/2013 09:38 MHH7131

Manganese (Mn) 0.166 mg/L 0.05 10 EPA 200.7 01/31/2013 09:38 MHH7131

DISSOLVED METALS BY ICP-MS

Selenium (Se) 22.0 ug/L 5 5 EPA 200.8 02/04/2013 15:29 KRICHAR

TOTAL RECOVERABLE METALS BY ICP-MS

Arsenic (As) < 5 ug/L 5 5 EPA 200.8 02/01/2013 15:20 KRICHAR

Cadmium (Cd) < 5 ug/L 5 5 EPA 200.8 02/01/2013 15:20 KRICHAR

Chromium (Cr) < 5 ug/L 5 5 EPA 200.8 02/01/2013 15:20 KRICHAR

Copper (Cu) < 5 ug/L 5 5 EPA 200.8 02/01/2013 15:20 KRICHAR

Nickel (Ni) < 5 ug/L 5 5 EPA 200.8 02/01/2013 15:20 KRICHAR

Selenium (Se) 23.2 ug/L 5 5 EPA 200.8 02/01/2013 15:20 KRICHAR

Silver (Ag) < 5 ug/L 5 5 EPA 200.8 02/01/2013 15:20 KRICHAR

Zinc (Zn) < 5 ug/L 5 5 EPA 200.8 02/01/2013 15:20 KRICHAR

SELENIUM SPECIATION - (Analysis Performed by Applied Speciation and Consulting, LLC)

Vendor Parameter Complete Vendor Method V_AS&C

Page 8 of 37
Certificate of Laboratory Analysis

This report shall not be reproduced, except in full.

Order # J13010488

2013002250

Collection Date: 25-Jan-13 8:28 AM

Site: BioReactor 2 Eff BLANK 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

MERCURY 1631 - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

MERCURY 1631 - DISSOLVED - (Analysis Performed by Brooks Rand Labs LLC)

Vendor Parameter Complete Vendor Method V_BRAND

2013002251

Collection Date: 25-Jan-13 10:20 AM

Site: Filter Blk 

Matrix: OTHER

Analyte Result Units Qualifiers RDL Method Analysis Date/Time

Sample #:

DF Analyst

DISSOLVED METALS BY ICP

Manganese (Mn) < 0.005 mg/L 0.005 1 EPA 200.7 02/05/2013 11:33 MHH7131

DISSOLVED METALS BY ICP-MS

Selenium (Se) < 1 ug/L 1 1 EPA 200.8 02/04/2013 14:38 KRICHAR

Page 9 of 37
This data package contains the analytical results for the project identified above and includes a Case Narrative, Sample 

Results and Chain of Custody. Unless otherwise noted, all samples were received in acceptable condition and processed 

according to the referenced methods. 

Data qualifiers are flagged individually on each sample. A key reference for the data qualifiers appears at the end of this case 

narrative.

NC Certification No. 402

SC Certification No. 99012

NC Drinking Water Cert No. 37735

Case Narrative

02/06/2013

Duke Energy Corporation

Jay Perkins

13339 Hagers Ferry Road

Huntersville, NC 28078

Project: Allen Wastewater - Nietering

Project No.: J13010488

Lab Submittal Date: 01/29/2013

Prism Work Order: 3010577

VA Certification No. 1287

DoD ELAP Certification No. L2307

Please call if you have any questions relating to this analytical report.

Respectfully,

PRISM LABORATORIES, INC.

VP Laboratory Services Reviewed By

Data Qualifiers Key Reference:

HT Sample received and analyzed outside of the hold time.

MDL Method Detection Limit

BRL Below Reporting Limit

RPD Relative Percent Difference

*

Results reported to the reporting limit. All other results are reported to the MDL with values between MDL and 

reporting limit indicated with a J.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 1 of 8

Page 10 of 37
Sample Receipt Summary

02/06/2013

Prism Work Order: 3010577

Client Sample ID Lab Sample ID Matrix Date Sampled Date Received

2013002243/FGD Purge Eff 3010577-01 Water 01/25/13 01/29/13

2013002245/BioReactor 1 Inf 3010577-02 Water 01/25/13 01/29/13

2013002247/BioReactor 2 Inf 3010577-03 Water 01/25/13 01/29/13

2013002249/BioReactor 2 Eff 3010577-04 Water 01/25/13 01/29/13

Samples received in good condition at 3.8 degrees C unless otherwise noted.

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 2 of 8

Page 11 of 37
Laboratory Report

02/06/2013

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation Project: Allen Wastewater - Nietering

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 3010577-01

Prism Work Order: 3010577

Time Collected: 01/25/13 08:20

Time Submitted: 01/29/13 15:00

2013002243/FGD Purge Eff

J13010488

General Chemistry Parameters

pH 6.7 HT pH Units 1 *SM4500-H B 2/4/13 11:00 JAB P3B0068

Total Alkalinity 76 mg/L 5.0 0.59 1 *SM2320 B 2/4/13 11:00 JAB P3B0045

Carbonate Alkalinity BRL mg/L 5.0 0.59 1 *SM2320 B 2/4/13 11:00 JAB P3B0046

Bicarbonate Alkalinity 76 mg/L 5.0 0.59 1 *SM2320 B 2/4/13 11:00 JAB P3B0047

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 3 of 8

Page 12 of 37
Laboratory Report

02/06/2013

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation Project: Allen Wastewater - Nietering

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 3010577-02

Prism Work Order: 3010577

Time Collected: 01/25/13 08:23

Time Submitted: 01/29/13 15:00

2013002245/BioReactor 1 Inf

J13010488

General Chemistry Parameters

pH 7.4 HT pH Units 1 *SM4500-H B 2/4/13 11:00 JAB P3B0068

Total Alkalinity 28 mg/L 5.0 0.59 1 *SM2320 B 2/4/13 11:00 JAB P3B0045

Carbonate Alkalinity BRL mg/L 5.0 0.59 1 *SM2320 B 2/4/13 11:00 JAB P3B0046

Bicarbonate Alkalinity 28 mg/L 5.0 0.59 1 *SM2320 B 2/4/13 11:00 JAB P3B0047

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 4 of 8

Page 13 of 37
Laboratory Report

02/06/2013

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation Project: Allen Wastewater - Nietering

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 3010577-03

Prism Work Order: 3010577

Time Collected: 01/25/13 08:31

Time Submitted: 01/29/13 15:00

2013002247/BioReactor 2 Inf

J13010488

General Chemistry Parameters

pH 7.1 HT pH Units 1 *SM4500-H B 2/4/13 11:00 JAB P3B0068

Total Alkalinity 110 mg/L 5.0 0.59 1 *SM2320 B 2/4/13 11:00 JAB P3B0045

Carbonate Alkalinity BRL mg/L 5.0 0.59 1 *SM2320 B 2/4/13 11:00 JAB P3B0046

Bicarbonate Alkalinity 110 mg/L 5.0 0.59 1 *SM2320 B 2/4/13 11:00 JAB P3B0047

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 5 of 8

Page 14 of 37
Laboratory Report

02/06/2013

Parameter Result Batch

ID

Report

Limit

Analysis

Date/Time

Dilution Method

Factor

Units MDL Analyst

13339 Hagers Ferry Road Project No.: 

Duke Energy Corporation Project: Allen Wastewater - Nietering

Huntersville, NC 28078

Attn: Jay Perkins

Sample Matrix: Water

Client Sample ID: 

Prism Sample ID: 3010577-04

Prism Work Order: 3010577

Time Collected: 01/25/13 08:28

Time Submitted: 01/29/13 15:00

2013002249/BioReactor 2 Eff

J13010488

General Chemistry Parameters

pH 6.7 HT pH Units 1 *SM4500-H B 2/4/13 11:00 JAB P3B0068

Total Alkalinity 54 mg/L 5.0 0.59 1 *SM2320 B 2/4/13 11:00 JAB P3B0045

Carbonate Alkalinity BRL mg/L 5.0 0.59 1 *SM2320 B 2/4/13 11:00 JAB P3B0046

Bicarbonate Alkalinity 54 mg/L 5.0 0.59 1 *SM2320 B 2/4/13 11:00 JAB P3B0047

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 6 of 8

Page 15 of 37
2/6/13

Level II QC Report

13339 Hagers Ferry Road Project No: J13010488

Duke Energy Corporation Project: Allen Wastewater - Nietering

Huntersville, NC 28078

Attn: Jay Perkins

Prism Work Order: 3010577

Time Submitted: 1/29/2013 3:00:00PM

Result Limit

Reporting

Units Level

Spike

Result

Source

%REC

%REC

Limits RPD

RPD

 Analyte Limit Notes 

General Chemistry Parameters - Quality Control

Batch P3B0045 - NO PREP

Blank (P3B0045-BLK1) Prepared & Analyzed: 02/04/13 

Total Alkalinity BRL 5.0 mg/L

LCS (P3B0045-BS1) Prepared & Analyzed: 02/04/13 

Total Alkalinity 248 5.0 mg/L 250.0 99 90-110

LCS Dup (P3B0045-BSD1) Prepared & Analyzed: 02/04/13 

Total Alkalinity 242 5.0 mg/L 250.0 97 90-110 3 200

Batch P3B0046 - NO PREP

Blank (P3B0046-BLK1) Prepared & Analyzed: 02/04/13 

Carbonate Alkalinity BRL 5.0 mg/L

Batch P3B0047 - NO PREP

Blank (P3B0047-BLK1) Prepared & Analyzed: 02/04/13 

Bicarbonate Alkalinity BRL 5.0 mg/L

LCS (P3B0047-BS1) Prepared & Analyzed: 02/04/13 

Bicarbonate Alkalinity 248 5.0 mg/L 250.0 99 90-110

LCS Dup (P3B0047-BSD1) Prepared & Analyzed: 02/04/13 

Bicarbonate Alkalinity 242 5.0 mg/L 250.0 97 90-110 3 200

Batch P3B0068 - NO PREP

LCS (P3B0068-BS1) Prepared & Analyzed: 02/04/13 

pH 6.88 pH Units 6.860 100 99-101

This report should not be reproduced, except in its entirety, without the written consent of Prism Laboratories, Inc.

449 Springbrook Road - P.O. Box 240543 - Charlotte, NC 28224-0543

Phone: 704/529-6364 - Toll Free Number: 1-800/529-6364 - Fax: 704/525-0409 Page 7 of 8

Page 16 of 37
Page 8 of 8

Page 17 of 37
3958 6th Ave NW • Seattle, WA 98107 • T: 206-632-6206 • F: 206-632-6017 • www.brooksrand.com • brl@brooksrand.com

February 19, 2013

Duke Energy

ATTN: Jay Perkins

Scientific Support-Laboratory

13339 Hagers Ferry Road

Huntersville NC 28078

jcperkins@duke-energy.com

labcustomer@duke-energy.com

RE: Project DUK-HV1201 Client Project: J13010488

Dear Mr. Perkins,

On February 1, 2013, Brooks Rand Labs (BRL) received three (3) wastewater sample and three

(3) corresponding field blanks. An aliquot was removed from each sample bottle and filtered into 

a separate container designed for dissolved mercury (Hg) analysis. The sample volume from 

the original container was logged-in for total Hg analysis. All samples were received, prepared, 

analyzed, and stored according to BRL SOPs and EPA methodology.

Data used for regulatory purposes has a 24 hour filtration holding time requirement. Nonregulatory purposed data has a 48 hour filtration holding time. The samples were received

outside of the non-regulatory requirement holding time and were qualified H. 

The results were blank-corrected as described in the calculations section of the relevant SOP

and may have been evaluated using reporting limits that have been adjusted to account for 

sample aliquot size. Please refer to the Sample Results page for sample-specific MDLs, MRLs, 

and other details. 

The results of continuing calibration blanks CCB7, CCB8, CCBC, and CCBD were greater than 

10 pg of Hg. All bracketed samples were re-analyzed for confirmation.

The analysis of the first method blank was determined to be a Grubb’s outlier with a result of 

0.73 ng/L. The result was omitted and all sample results were method blank-corrected by the 

average of the three remaining method blank results. All data was reported without additional 

qualification, aside from concentration qualifiers, and all associated quality control sample 

results met the acceptance criteria.

BRL, an accredited laboratory, certifies the reported results of all analyses for which BRL is 

NELAP accredited meet all NELAP requirements. For more details, see the Report Information

page of the report. Please feel free to contact me if you have any questions.

Sincerely,

 

Tiffany Stilwater 

Project Manager 

tiffany@brooksrand.com 

Page 18 of 37
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Definition of Data Qualifiers

(Effective 9/23/09)

Laboratory Accreditation

BRL is accredited by the National Environmental Laboratory Accreditation Program (NELAP) through the State of Florida 

Department of Health, Bureau of Laboratories (E87982) and is certified to perform many environmental analyses. BRL is 

also certified by many other states to perform environmental analyses. For a current list of our accreditations /certifications, 

please visit our website at <http://www.brooksrand.com/default.asp?contentID=586>. Results reported relate only to the 

samples listed in the report.

Report Information

BLK

BRL

BS

CAL

CCV

CRM

D

DUP

ICV

MSD

ND

NR

PS

REC

RPD

RSD

SCV

SOP

method blank 

Brooks Rand Labs

laboratory fortified blank

calibration standard

continuing calibration verification

certified reference material

dissolved fraction

duplicate

initial calibration verification

matrix spike duplicate

non-detect

non-reportable

post preparation spike

percent recovery

relative percent difference

relative standard deviation

secondary calibration verification

standard operating procedure

MDL

MRL

MS

method detection limit

method reporting limit

matrix spike

SRM

T

COC

standard reference material

total recoverable fraction

chain of custody record 

Common Abbreviations

These qualifiers are based on those previously utilized by Brooks Rand Labs, those found in the EPA SOW ILM03.0, 

Exhibit B, Section III, pg. B-18, and the USEPA Contract Laboratory Program National Functional Guidelines for Inorganic 

Superfund Data Review; USEPA; January 2010. These supersede all previous qualifiers ever employed by BRL.

B Detected by the instrument, the result is > the MDL but ≤ the MRL. Result is reported and considered an estimate.

E An estimated value due to the presence of interferences. A full explanation is presented in the narrative.

H Holding time and/or preservation requirements not met. Result is estimated.

J Estimated value. A full explanation is presented in the narrative.

J-M Duplicate precision (RPD) for associated QC sample was not within acceptance criteria. Result is estimated.

J-N Spike recovery for associated QC sample was not within acceptance criteria. Result is estimated.

M Duplicate precision (RPD) was not within acceptance criteria. Result is estimated.

N Spike recovery was not within acceptance criteria. Result is estimated.

R Rejected, unusable value. A full explanation is presented in the narrative.

U Result is ≤ the MDL or client requested reporting limit (CRRL). Result reported as the MDL or CRRL.

X Result is not BLK-corrected and is within 10x the absolute value of the highest detectable BLK in the batch. 

Result is estimated.

Field Quality Control Samples

Please be notified that certain EPA methods require the collection of field quality control samples of an appropriate type 

and frequency; failure to do so is considered a deviation from some methods and for compliance purposes should only be 

done with the approval of regulatory authorities. Please see the specific EPA methods for details regarding required field 

quality control samples.

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 19 of 37
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Information

Sample Lab ID Report Matrix Type Sampled Received

BioReactor 1 Inf 1305019-01 Influent Sample 01/25/2013 02/01/2013

BioReactor 1 Inf 1305019-02 Influent Sample 01/25/2013 02/01/2013

BioReactor 1 Inf Hg Blk 1305019-03 DIW Field Blank 01/25/2013 02/01/2013

BioReactor 1 Inf Hg Blk 1305019-04 DIW Field Blank 01/25/2013 02/01/2013

BioReactor 2 Inf 1305019-05 Influent Sample 01/25/2013 02/01/2013

BioReactor 2 Inf 1305019-06 Influent Sample 01/25/2013 02/01/2013

BioReactor 2 Inf Hg Blk 1305019-07 DIW Field Blank 01/25/2013 02/01/2013

BioReactor 2 Inf Hg Blk 1305019-08 DIW Field Blank 01/25/2013 02/01/2013

BioReactor 2 Eff 1305019-09 Effluent Sample 01/25/2013 02/01/2013

BioReactor 2 Eff 1305019-10 Effluent Sample 01/25/2013 02/01/2013

BioReactor 2 Eff Hg Blk 1305019-11 DIW Field Blank 01/25/2013 02/01/2013

BioReactor 2 Eff Hg Blk 1305019-12 DIW Field Blank 01/25/2013 02/01/2013

Batch Summary

Analyte Lab Matrix Method Prepared Analyzed Batch Sequence

Hg Water EPA 1631 02/08/2013 02/11/2013 B130200 1300095

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 20 of 37
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Results

Sample Analyte Report Matrix Basis Result Qualifier MDL MRL Unit Batch Sequence

BioReactor 1 Inf

1305019-01 Hg Influent T 9670 75.8 202 ng/L B130200 1300095

1305019-02 Hg Influent D 9000 H 37.9 101 ng/L B130200 1300095

BioReactor 1 Inf Hg Blk

1305019-03 Hg DIW T 0.15 U 0.15 0.39 ng/L B130200 1300095

1305019-04 Hg DIW D 0.15 H, U 0.15 0.40 ng/L B130200 1300095

BioReactor 2 Eff

1305019-09 Hg Effluent T 36.2 0.15 0.41 ng/L B130200 1300095

1305019-10 Hg Effluent D 34.0 H 0.15 0.41 ng/L B130200 1300095

BioReactor 2 Eff Hg Blk

1305019-11 Hg DIW T 0.15 U 0.15 0.41 ng/L B130200 1300095

1305019-12 Hg DIW D 0.15 H, U 0.15 0.40 ng/L B130200 1300095

BioReactor 2 Inf

1305019-05 Hg Influent T 147 0.38 1.02 ng/L B130200 1300095

1305019-06 Hg Influent D 15.3 H 0.16 0.42 ng/L B130200 1300095

BioReactor 2 Inf Hg Blk

1305019-07 Hg DIW T 0.16 U 0.16 0.41 ng/L B130200 1300095

1305019-08 Hg DIW D 0.15 H, U 0.15 0.41 ng/L B130200 1300095

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 21 of 37
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Accuracy & Precision Summary

Batch: B130200

Analyte Native Spike Result Units REC & Limits RPD & Limits

Method: EPA 1631

Lab Matrix: Water

Sample

B130200-SRM1 Certified Reference Material (1307035, NIST 1641d 1000x dilution)

Hg 15.68 16.08 ng/L 103% 85-115

B130200-MS3 Matrix Spike (1305017-01)

Hg 17310 50510 73420 ng/L 111% 71-125

B130200-MSD3 Matrix Spike Duplicate (1305017-01)

Hg 17310 50510 74470 ng/L 113% 71-125 241%

B130200-MS2 Matrix Spike (1305020-01)

Hg 278.6 606.1 942.9 ng/L 110% 71-125

B130200-MSD2 Matrix Spike Duplicate (1305020-01)

Hg 278.6 606.1 940.3 ng/L 109% 71-125 240.3%

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 22 of 37
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Method Blanks & Reporting Limits

Batch: B130200

Method: EPA 1631

Matrix: Water

Analyte: Hg

Sample Result Units

B130200-BLK2 0.18 ng/L

B130200-BLK3 0.21 ng/L

B130200-BLK4 0.20 ng/L

Average: 0.20 Standard Deviation: 0.02 MDL: 0.15

Limit: 0.50 Limit: 0.10 MRL: 0.39

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 23 of 37
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Instrument Calibration

Sequence: 1300095

Instrument: THG-05 Method: EPA 1631

Date: 02/11/2013

Total Mercury and Mercury Speciation by CVAFS

Analyte: Hg

Lab ID True Value Result Units REC & Limits

1300095-IBL1 0.78 pg of Hg

1300095-IBL2 2.35 pg of Hg

1300095-IBL3 2.61 pg of Hg

1300095-IBL4 2.86 pg of Hg

1300095-CAL1 10.00 10.06 pg of Hg 101%

1300095-CAL2 25.00 24.27 pg of Hg 97%

1300095-CAL3 100.0 98.89 pg of Hg 99%

1300095-CAL4 500.0 499.0 pg of Hg 100%

1300095-CAL5 2500 2521 pg of Hg 101%

1300095-CAL6 10000 10300 pg of Hg 103%

1300095-ICV1 1568 1608 pg of Hg 103% 85-115

1300095-CCB1 7.23 pg of Hg

1300095-CCV1 500.0 515.4 pg of Hg 103% 77-123

1300095-CCB2 4.67 pg of Hg

1300095-CCB3 4.47 pg of Hg

1300095-CCB4 4.28 pg of Hg

1300095-CCV2 500.0 529.5 pg of Hg 106% 77-123

1300095-CCB5 4.02 pg of Hg

1300095-CCV3 500.0 532.5 pg of Hg 107% 77-123

1300095-CCB6 3.81 pg of Hg

1300095-CCV4 500.0 592.1 pg of Hg 118% 77-123

1300095-CCB7 38.4 pg of Hg

1300095-CCV5 500.0 562.1 pg of Hg 112% 77-123

1300095-CCB8 21.0 pg of Hg

1300095-CCV6 500.0 544.2 pg of Hg 109% 77-123

1300095-CCB9 9.78 pg of Hg

1300095-CCV7 500.0 525.3 pg of Hg 105% 77-123

1300095-CCBA 6.09 pg of Hg

1300095-CCV8 500.0 526.2 pg of Hg 105% 77-123

1300095-CCBB 5.08 pg of Hg

1300095-CCV9 500.0 530.5 pg of Hg 106% 77-123

1300095-CCBC 13.1 pg of Hg

1300095-CCVA 500.0 526.5 pg of Hg 105% 77-123

1300095-CCBD 15.9 pg of Hg

1300095-CCVB 500.0 523.1 pg of Hg 105% 77-123

1300095-CCBE 6.43 pg of Hg

1300095-CCVC 500.0 529.5 pg of Hg 106% 77-123

1300095-CCBF 6.87 pg of Hg

1300095-ICV2 1568 1662 pg of Hg 106% 85-115

1300095-CCVD 500.0 544.6 pg of Hg 109% 77-123

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 24 of 37
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Instrument Calibration

Sequence: 1300095

Instrument: THG-05 Method: EPA 1631

Date: 02/11/2013

Total Mercury and Mercury Speciation by CVAFS

Analyte: Hg

Lab ID True Value Result Units REC & Limits

1300095-CCBG 5.21 pg of Hg

1300095-CCVE 500.0 569.9 pg of Hg 114% 77-123

1300095-CCBH 5.66 pg of Hg

1300095-CCVF 500.0 578.9 pg of Hg 116% 77-123

1300095-CCBI 5.45 pg of Hg

1300095-CCVG 500.0 590.1 pg of Hg 118% 77-123

1300095-CCBJ 7.04 pg of Hg

1300095-CCVH 500.0 574.2 pg of Hg 115% 77-123

1300095-CCBK 5.11 pg of Hg

1300095-CCVI 500.0 568.7 pg of Hg 114% 77-123

1300095-CCBL 3.90 pg of Hg

1300095-CCVJ 500.0 571.9 pg of Hg 114% 77-123

1300095-CCBM 4.30 pg of Hg

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 25 of 37
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Containers

Lab ID: 1305019-01 Report Matrix: Influent

Sample: BioReactor 1 Inf Sample Type: Sample Received: 02/01/2013

Collected: 01/25/2013

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71666330

10

Bottle FLPE Hg-T 500mL none n/a Cooler

Lab ID: 1305019-02 Report Matrix: Influent

Sample: BioReactor 1 Inf Sample Type: Sample Received: 02/01/2013

Collected: 01/25/2013

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A Bottle FLPE Hg-T 250mL 13-0001 none n/a Cooler

Lab ID: 1305019-03 Report Matrix: DIW

Sample: BioReactor 1 Inf Hg Blk Sample Type: Field Blank Received: 02/01/2013

Collected: 01/25/2013

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71666330

10

Bottle FLPE Hg-T 500mL none n/a Cooler

Lab ID: 1305019-04 Report Matrix: DIW

Sample: BioReactor 1 Inf Hg Blk Sample Type: Field Blank Received: 02/01/2013

Collected: 01/25/2013

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A Bottle FLPE Hg-T 250mL 13-0001 none n/a Cooler

Lab ID: 1305019-05 Report Matrix: Influent

Sample: BioReactor 2 Inf Sample Type: Sample Received: 02/01/2013

Collected: 01/25/2013

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71666330

10

Bottle FLPE Hg-T 500mL none n/a Cooler

Lab ID: 1305019-06 Report Matrix: Influent

Sample: BioReactor 2 Inf Sample Type: Sample Received: 02/01/2013

Collected: 01/25/2013

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A Bottle FLPE Hg-T 250mL 13-0001 none n/a Cooler

Lab ID: 1305019-07 Report Matrix: DIW

Sample: BioReactor 2 Inf Hg Blk Sample Type: Field Blank Received: 02/01/2013

Collected: 01/25/2013

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71666330

10

Bottle FLPE Hg-T 500mL none n/a Cooler

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 26 of 37
Project ID: DUK-HV1201

PM: Tiffany Stilwater

Client PM: Jay Perkins

 Client PO: 141391

Sample Containers

Lab ID: 1305019-08 Report Matrix: DIW

Sample: BioReactor 2 Inf Hg Blk Sample Type: Field Blank Received: 02/01/2013

Collected: 01/25/2013

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A Bottle FLPE Hg-T 250mL 13-0001 none n/a Cooler

Lab ID: 1305019-09 Report Matrix: Effluent

Sample: BioReactor 2 Eff Sample Type: Sample Received: 02/01/2013

Collected: 01/25/2013

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71666330

10

Bottle FLPE Hg-T 500mL none n/a Cooler

Lab ID: 1305019-10 Report Matrix: Effluent

Sample: BioReactor 2 Eff Sample Type: Sample Received: 02/01/2013

Collected: 01/25/2013

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A Bottle FLPE Hg-T 250mL 13-0001 none n/a Cooler

Lab ID: 1305019-11 Report Matrix: DIW

Sample: BioReactor 2 Eff Hg Blk Sample Type: Field Blank Received: 02/01/2013

Collected: 01/25/2013

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A 71666330

10

Bottle FLPE Hg-T 500mL none n/a Cooler

Lab ID: 1305019-12 Report Matrix: DIW

Sample: BioReactor 2 Eff Hg Blk Sample Type: Field Blank Received: 02/01/2013

Collected: 01/25/2013

Des Container Size Lot Preservation P-Lot pH Ship. Cont.

A Bottle FLPE Hg-T 250mL 13-0001 none n/a Cooler

Shipping Containers

Cooler

Tracking No: 535305198114 via FedEx

Temperature: 0.4 °C

Coolant Type: Ice

Description: Cooler

Damaged in transit? No

Returned to client? No

Custody seals present? No

Custody seals intact? No

COC present? Yes

Received: February 1, 2013 9:25

3958 6th Avenue NW Seattle WA 98107 · P(206) 632-6206 · F(206) 632-6017 · brl@brooksrand.com · www.brooksrand.com

Page 27 of 37
Page 28 of 37
 

February 12, 2013 

Jay Perkins 

Duke Energy Analytical Laboratory 

Mail Code MGO3A2 (Building 7405) 

13339 Hagers Ferry Rd. 

Huntersville, NC 28078 

(704) 875-5245 

Project: Allen Wastewater – Nietering (January 2013 – Test Burn) (LIMS #J13010488) 

Dear Mr. Perkins, 

Attached is the report associated with four (4) aqueous samples submitted for selenium 

speciation on January 31, 2013. The samples were received in a sealed cooler at -0.5°C on 

February 1, 2013. Selenium speciation analysis was performed via ion chromatography 

inductively coupled plasma collision reaction cell mass spectrometry (IC-ICP-CRC-MS). Any 

issues associated with the analysis are addressed in the following report. 

If you have any questions, please feel free to contact me at your convenience. 

 Sincerely, 

 

 Russell Gerads 

Vice President 

Applied Speciation and Consulting, LLC 

 

 

Page 29 of 37
Applied Speciation and Consulting, LLC 

Report prepared for: 

Jay Perkins 

Duke Energy Analytical Laboratory 

Mail Code MGO3A2 (Building 7405) 

13339 Hagers Ferry Rd. 

Huntersville, NC 28078 

Project: Allen Wastewater – Nietering (January 2013 – Test Burn) (LIMS #J13010488) 

February 12, 2013 

1. Sample Reception 

Four (4) aqueous samples in 125mL HDPE bottles (provided by Applied Speciation and 

Consulting) were submitted for selenium speciation analysis on January 31, 2013. The 

samples were received on February 1, 2013 in a sealed container at -0.5°C. 

The samples were received in a laminar flow clean hood, void of trace metals contamination 

and ultra-violet radiation, and were designated discrete sample identifiers. An aliquot of 

each sample was filtered (0.45μm) and each filtrate was stored in a secure, monitored 

cryofreezer (maintained at a temperature of -80oC) until selenium speciation analysis could 

be performed via ion chromatography inductively coupled plasma collision reaction cell 

mass spectrometry (IC-ICP-CRC-MS). 

2. Sample Preparation 

All sample preparation is performed in laminar flow clean hoods known to be free from trace 

metals contamination. All applied water for dilutions and sample preservatives are 

monitored for contamination to account for any biases associated with the sample results. 

Selenium Speciation Analysis by IC-ICP-CRC-MS Prior to analysis, an aliquot of each 

sample was filtered with a syringe filter (0.45μm) and injected directly into an autosampler 

vial. No further sample preparation was performed as any chemical alteration of a sample 

may shift the equilibrium of the system, resulting in changes in speciation ratios. 

3. Sample Analysis 

All sample analysis is preceded by a minimum of a five-point calibration curve spanning the 

entire concentration range of interest. Calibration curves are performed at the beginning of 

Page 30 of 37
each analytical day. All calibration curves, associated with each species of interest, are 

standardized by linear regression resulting in a response factor. All sample results are 

instrument blank corrected to account for any operational biases associated with the 

analytical platform. 

Prior to sample analysis, all calibration curves are verified using second source standards 

which are identified as initial calibration verification standards (ICV). 

Ongoing instrument performance is identified by the analysis of continuing calibration 

verification standards (CCV) and continuing calibration blanks (CCB) at a minimum interval 

of every ten analytical runs. 

Selenium Speciation Analysis by IC-ICP-CRC-MS Each sample for selenium speciation 

analysis was analyzed by ion chromatography inductively coupled plasma collision reaction 

cell mass spectrometry (IC-ICP-CRC-MS) on February 9, 2013. An aliquot of each sample 

is injected onto an anion exchange column and mobilized by a basic (pH > 7) gradient. The 

eluting selenium species are then introduced into a radio frequency (RF) plasma where 

energy-transfer processes cause desolvation, atomization, and ionization. The ions are 

extracted from the plasma through a differentially-pumped vacuum interface and travel 

through a pressurized chamber (CRC) containing a reaction gas which preferentially reacts 

with interfering ions of the same target mass to charge ratios (m/z). A solid-state detector 

detects ions transmitted through the mass analyzer and the resulting current is processed by a 

data handling system. 

Retention times for each eluting species are compared to known standards for species 

identification. 

4. Analytical Issues 

The overall analyses went well and no significant analytical issues were encountered. All 

quality control parameters associated with the samples were within acceptance limits. 

The estimated method detection limits (eMDLs) for selenite, selenate, and selenocyanate are 

generated from replicate analyses of the lowest standard in the calibration curve. Not all 

selenium species are present in preparation blanks; therefore, eMDL calculations based on 

preparation blanks are artificially biased low. 

The eMDL for methylseleninic acid and selenomethionine is calculated from the average 

eMDL of selenite, selenate, and selenocyanate. The calibration does not contain 

methylseleninic acid or selenomethionine due to impurities in these standards which would 

bias the results for other selenium species. 

Page 31 of 37
The laboratory control sample (LCS) recoveries for methylseleninic acid and 

selenomethionine were elevated (132.2% and 130.2%, respectively). The speciation ratios 

identify that the referenced selenium species are relatively non-contributory with respect to 

the other selenium species present in the samples. Since the perceived elevated responses for 

methylseleninic acid and selenomethionine had negligible impact on the reported results no 

corrective action was taken. 

If you have any questions or concerns regarding this report, please feel free to contact me. 

Sincerely, 

Russell Gerads 

Vice President 

Applied Speciation and Consulting, LLC 

Page 32 of 37
Sample Results

Sample ID Se(IV) Se(VI) SeCN MeSe(IV) SeMe

Unknown Se 

Species (n)

FGD Purge Eff 25.1 440 ND (<2.9) 6.0 ND (<3.1) 0 (0)

BioReactor 1 Inf 58.7 396 ND (<0.73) 6.96 ND (<0.79) 0.74

BioReactor 2 Inf 33.7 14.5 ND (<0.73) 1.28 ND (<0.79) 0 (0)

BioReactor 2 Eff 0.85 ND (<0.87) ND (<0.73) ND (<0.79) ND (<0.79) 0 (0)

All results reflect the applied dilution and are reported in μg/L

ND = Not detected at the applied dilution

SeCN = Selenocyanate

MeSe(IV) = Methylseleninic acid

SeMe = Selenomethionine

Unknown Se Species = Total concentration of all unknown Se species observed by IC-ICP-MS

Report Generated by: Russell Gerads

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: Allen Wastewater - Nietering (January 2013 - Test Burn)

Contact: Jay Perkins

Date: February 12, 2013

LIMS #J13010488

Page 33 of 37
Quality Control Summary - Preparation Blank Summary

Analyte (μg/L) PBW1 PBW2 PBW3 PBW4 Mean StdDev eMDL* eMDL 250x eMDL 1000x

Se(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.003 0.76 3.0

Se(VI) 0.000 0.000 0.000 0.000 0.000 0.000 0.003 0.87 3.5

SeCN 0.000 0.000 0.000 0.000 0.000 0.000 0.003 0.73 2.9

MeSe(IV) 0.000 0.000 0.000 0.000 0.000 0.000 0.003 0.79 3.1

SeMe 0.000 0.000 0.000 0.000 0.000 0.000 0.003 0.79 3.1

eMDL = Estimated Method Detection Limit

*Please see narrative regarding eMDL calculations

Quality Control Summary - Certified Reference Materials

Analyte (μg/L) CRM True Value Result Recovery

Se(IV) LCS 9.57 9.34 97.6

Se(VI) LCS 9.48 8.93 94.2

SeCN LCS 8.92 8.53 95.6

MeSe(IV) LCS 6.47 8.55 132.2

SeMe LCS 9.32 12.14 130.2

Selenium Speciation Results for Duke Energy

Project Name: Allen Wastewater - Nietering (January 2013 - Test Burn)

Contact: Jay Perkins

LIMS #J13010488

Date: February 12, 2013

Report Generated by: Russell Gerads

Applied Speciation and Consulting, LLC

Page 34 of 37
Quality Control Summary - Matrix Duplicates

Analyte (μg/L) Sample ID Rep 1 Rep 2 Mean RPD

Se(IV) Batch QC 88.64 87.48 88.1 1.3

Se(VI) Batch QC 62.0 62.3 62.2 0.5

SeCN Batch QC ND (<0.73) ND (<0.73) NC NC

MeSe(IV) Batch QC 2.3 1.8 2.0 23.7

SeMe Batch QC ND (<0.79) ND (<0.79) NC NC

ND = Not detected at the applied dilution

NC = Value was not calculated due to one or more concentrations below the eMDL

Quality Control Summary - Matrix Spike/ Matrix Spike Duplicate

Analyte (μg/L) Sample ID Spike Conc MS Result Recovery Spike Conc MSD Result Recovery RPD

Se(IV) Batch QC 1390 1511 102.3 1390 1501 101.7 0.6

Se(VI) Batch QC 1261 1316 99.4 1261 1303 98.4 1.0

SeCN Batch QC 1144 1112 97.2 1144 1123 98.2 1.0

Applied Speciation and Consulting, LLC

Selenium Speciation Results for Duke Energy

Project Name: Allen Wastewater - Nietering (January 2013 - Test Burn)

Contact: Jay Perkins

LIMS #J13010488

Date: February 12, 2013

Report Generated by: Russell Gerads

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