# Patent

## Title
Variant CBH I polypeptides with reduced product inhibition

## Abstract

The present disclosure relates to variant CBH I polypeptides that have reduced product inhibition, and compositions, e.g., cellulase compositions, comprising variant CBH I polypeptides. The variant CBH I polypeptides and related compositions can be used in variety of agricultural and industrial applications. The present disclosure further relates to nucleic acids encoding variant CBH I polypeptides and host cells that recombinantly express the variant CBH I polypeptides.

## Background
CROSS-REFERENCE TO RELATED APPLICATIONS
The present application claims the benefit of national stage application filed in compliance with 35 U.S.C. §371 of International Application No. PCT/US2011/055181, filed Oct. 6, 2011, which claims benefit under 35 U.S.C. §119(3) of U.S. Provisional Application No. 61/390,392, filed Oct. 6, 2010, and are herein incorporated in their entireties for all purposes.


BACKGROUND
Cellulose is an unbranched polymer of glucose linked by β(1→4)-glycosidic bonds. Cellulose chains can interact with each other via hydrogen bonding to form a crystalline solid of high mechanical strength and chemical stability. The cellulose chains are depolymerized into glucose and short oligosaccharides before organisms, such as the fermenting microbes used in ethanol production, can use them as metabolic fuel. Cellulase enzymes catalyze the hydrolysis of the cellulose (hydrolysis of β-1,4-D-glucan linkages) in the biomass into products such as glucose, cellobiose, and other cellooligosaccharides. Cellulase is a generic term denoting a multienzyme mixture comprising exo-acting cellobiohydrolases (CBHs), endoglucanases (EGs) and β-glucosidases (BGs) that can be produced by a number of plants and microorganisms. Enzymes in the cellulase of Trichoderma reesei include CBH I (more generally, Cel7A), CBH2 (Cel6A), EG1 (Cel7B), EG2 (Cel5), EG3 (Cel12), EG4 (Cel61A), EG5 (Cel45A), EG6 (Cel74A), Cip1, Cip2, β-glucosidases (including, e.g., Cel3A), acetyl xylan esterase, β-mannanase, and swollenin.
Cellulase enzymes work synergistically to hydrolyze cellulose to glucose. CBH I and CBH II act on opposing ends of cellulose chains (Barr et al., 1996, Biochemistry 35:586-92), while the endoglucanases act at internal locations in the cellulose. The primary product of these enzymes is cellobiose, which is further hydrolyzed to glucose by one or more β-glucosidases.
The cellobiohydrolases are subject to inhibition by their direct product, cellobiose, which results in a slowing down of saccharification reactions as product accumulates. There is a need for new and improved cellobiohyrolases with improved productivity that maintain their reaction rates during the course of a saccharification reaction, for use in the conversion of cellulose into fermentable sugars and for related fields of cellulosic material processing such as pulp and paper, textiles and animal feeds.
SUMMARY
The present disclosure relates to variant CBH 1 polypeptides. Most naturally occurring CBH 1 polypeptides have arginines at positions corresponding to R268 and R411 of T. reesei CBH I (SEQ ID NO:2). The variant CBH I polypeptides of the present disclosure include a substitution at either or both positions resulting in a reduction or decrease in product (e.g., cellobiose) inhibition. Such variants are sometimes referred to herein as “product tolerant.”
The variant CBH I polypeptides of the disclosure minimally contain at least a CBH I catalytic domain, comprising (a) a substitution at the amino acid position corresponding to R268 of T. reesei CBH I (“R268 substitution”); (b) a substitution at the amino acid position corresponding to R411 of T. reesei CBH I (“R411 substitution”); or (c) both an R268 substitution and an R411 substitution. The amino acid positions of exemplary CBH I polypeptides into which R268 and/or R411 substitutions can be introduced are shown in Table 1, and the amino acid positions corresponding to 8268 and/or R411 in these exemplary CBH I polypeptides are shown in Table 2.
R268 and/or R411 substituents can include lysines and/or alanines. Accordingly, the present disclosure provides a variant CBH I polypeptide comprising a CBH I catalytic domain with one of the following amino acid substitutions or pairs of R268 and/or R411 substitutions: (a) R268K and R411K; (b) R268K and R411A; (c) R268A and R411K; (d) R268A and R411A; (e) R268A; (0 R268K; (g) R411A; and (h) R411K. In some embodiments, however, the amino acid sequence of the variant CBH I polypeptide does not comprise or consist of SEQ ID NO:299, SEQ ID NO:300, SEQ ID NO:301, or SEQ ID NO:302.
The variant CBHI polypeptides of the disclosure typically include a CD comprising an amino acid sequence having at least 50% sequence identity to a CD of a reference CBH I exemplified in Table 1. The CD portions of the CBH I polypeptides exemplified in Table 1 are delineated in Table 3. The variant CBH I polypeptides can have a cellulose binding exemplified in Table 1. The CD portions of the CBH I polypeptides exemplified in Table 1 are delineated in Table 3. The variant CBH I polypeptides can have a cellulose binding domain (“CBD”) sequence in addition to the catalytic domain (“CD”) sequence. The CBD can be N- or C-terminal to the CD, and the CBD and CD are optionally connected via a linker sequence.
The variant CBH I polypeptides can be mature polypeptides or they may further comprise a signal sequence.
Additional embodiments of the variant CBH I polypeptides are provided in Section 5.1.
The variant CBH I polypeptides of the disclosure typically exhibit reduced product inhibition by cellobiose. In certain embodiments, the IC50 of cellobiose towards a variant CBH I polypeptide of the disclosure is at least 1.2-fold, at least 1.5-fold, or at least 2-fold the IC50 of cellobiose towards a reference CBH I lacking the R268 substitution and/or R411 substitution present in the variant. Additional embodiments of the product inhibition characteristics of the variant CBH I polypeptides are provided in Section 5.1.
The variant CBH I polypeptides of the disclosure typically retain some cellobiohydrolase activity. In certain embodiments, a variant CBH I polypeptide retains at least 50% the CBH I activity of a reference CBH I lacking the R268 substitution and/or R411 substitution present in the variant. Additional embodiments of cellobiohydrolase activity of the variant CBH I polypeptides are provided in Section 5.1.
The present disclosure further provides compositions (including cellulase compositions, e.g., whole cellulase compositions, and fermentation broths) comprising variant CBH I polypeptides. Additional embodiments of compositions comprising variant CBH I polypeptides are provided in Section 5.3. The variant CBH I polypeptides and compositions comprising them can be used, inter alia, in processes for saccharifying biomass. Additional details of saccharification reactions, and additional applications of the variant CBH I polypeptides, are provided in Section 5.4.
The present disclosure further provides nucleic acids (e.g., vectors) comprising nucleotide sequences encoding variant CBH I polypeptides as described herein, and recombinant cells engineered to express the variant CBH I polypeptides. The recombinant cell can be a prokaryotic (e.g., bacterial) or eukaryotic (e.g., yeast or filamentous fungal) cell. Further provided are methods of producing and optionally recovering the variant CBH I polypeptides. Additional embodiments of the recombinant expression system suitable for expression and production of the variant CBH I polypeptides are provided in Section 5.2.



BRIEF DESCRIPTION OF THE FIGURES AND TABLES
FIG. 1A-1B: Cellobiose dose-response curves using a 4-MUL assay for a wild-type CBH I (BD29555; FIG. 1A) and a R268K/R411K variant CBH I (BD29555 with the substitutions R273K/R422K; FIG. 1B).
FIG. 2A-2B: The effect of cellobiose accumulation on the activity of wild-type CBH I and a R268K/R411K variant CBH I, based on percent conversion of glucan after 72 hours in the bagasse assay. FIG. 2A shows relative activity in the presence (+) and absence (−) of β-glucosidase (BG), where relative activity is normalized to wild type activity with BG (WT+=1). FIG. 2B shows tolerance to cellobiose as a function of the ratio of activity in the absence vs. presence of β-glucosidase (activity ratio=Activity−BG/Activity+BG).
FIG. 3: Cellobiose dose-response curves using PASC assay for a R268K/R411K variant CBH I polypeptide as compared to two wild type CBH I polypeptides.
FIG. 4: The effect of cellobiose accumulation on the activity of a wild-type CBH I and a R268K/R411K variant CBH I based on percent conversion of glucan after 72 hours in the bagasse assay in the presence (+) and absence (−) of β-glucosidase (BG). Activity is normalized to wild type activity with BG (WT+=1).
FIG. 5: Characterization of cellobiose product tolerance of variant CBH I polypeptides, based on percent conversion of glucan after 72 hours in the absence and presence of β-glucosidase (BG) in the bagasse assay; tolerance is evaluated as a function of the ratio of activity in the absence vs. presence of β-glucosidase.



TABLE 1: Amino acid sequences of exemplary “reference” CBH I polypeptides that can be modified at positions corresponding to R268 and/or R411 in T. reesei CBH I (SEQ ID NO:2). The database accession numbers are indicated in the second column. Unless indicated otherwise, the accession numbers refer to the Genbank database. “#” indicates that the CBH I has no signal peptide; “&” indicate that the sequence is from the PDB database and represents the catalytic domain only without signal sequence; * indicates a nonpublic database. These amino acid sequences are mostly wild type, with the exception of some sequences from the PDB database which contain mutations to facilitate protein crystallization.
TABLE 2: Amino acid positions in the exemplary reference CBH I polypeptides that correspond to R268 and R411 in T. reesei CBH I. Database descriptors are as for Table 1.
TABLE 3: Approximate amino acid positions of CBH I polypeptide domains. Abbreviations used: SS is signal sequence; CD is catalytic domain; and CBD is cellulose binding domain. Database descriptors are as for Table 1.
TABLE 4: Table 4 shows a segment within the catalytic domain of each exemplary reference CBH I polypeptide containing the active site loop (shown in bold, underlined text) and the catalytic residues (glutamates in most CBH I polypeptides) (shown in bold, double underlined text). Database descriptors are as for Table 1.
TABLE 5: MUL and bagasse assay results for variants of BD29555. ND means not determined. ±% Activity (+/−cellobiose)=[(Activity with cellobiose)/(Activity without cellobiose)]*100. ¥% Activity (−/+BG)=[(Activity without BG)/(Activity with BG)]*100]
TABLE 6: MUL and bagasse assay results for variants of T. reesei CBH I. ND means not determined. ±% Activity (+/−cellobiose)=[(Activity with cellobiose)/(Activity without cellobiose)]*100. ¥% Activity (−/+BG)=[(Activity without BG)/(Activity with BG)]*100.
TABLE 7: Informal sequence listing. SEQ ID NO:1-149 correspond to the exemplary reference CBH I polypeptides. SEQ ID NO:299 corresponds to mature T. reesei CBH I (amino acids 26-529 of SEQ ID NO:2) with an R268A substitution. SEQ ID NO:300 corresponds to mature T. reesei CBH I (amino acids 26-529 of SEQ ID NO:2) with an R411A substitution. SEQ ID NO:301 corresponds to full length BD29555 with both an R268K substitution and an R411K substitution. SEQ ID NO:302 corresponds to mature BD29555 with both an R268K substitution and an R411K substitution. corresponds to mature T. reesei CBH I (amino acids 26-529 of SEQ ID NO:2) with an R411A substitution. SEQ ID NO:152 corresponds to full length BD29555 with both an R268K substitution and an R411K substitution. SEQ ID NO:153 corresponds to mature BD29555 with both an R268K substitution and an R411K substitution.
DETAILED DESCRIPTION
The present disclosure relates to variant CBH I polypeptides. Most naturally occurring CBH I polypeptides have arginines at positions corresponding to R268 and R411 of T. reesei CBH I (SEQ ID NO:2). The variant CBH I polypeptides of the present disclosure include a substitution at either or both positions resulting in a reduction of product (e.g., cellobiose) inhibition. The following subsections describe in greater detail the variant CBH I polypeptides and exemplary methods of their production, exemplary cellulase compositions comprising them, and some industrial applications of the polypeptides and cellulase compositions.
Variant CBH I Polypeptides
The present disclosure provides variant CBH I polypeptides comprising at least one amino acid substitution that results in reduced product inhibition. “Variant” means a polypeptide which is differs in sequence from a reference polypeptide by substitution of one or more amino acids at one or a number of different sites in the amino acid sequence. Exemplary reference CBH I polypeptides are shown in Table 1.
The variant CBH I polypeptides of the disclosure have an amino acid substitution at the amino acid position corresponding to R268 of T. reesei CBH I (SEQ ID NO:2) (an “R268 substitution”), (b) a substitution at the amino acid position corresponding to R411 of T. reesei CBH I (“R411 substitution”); or (c) both an R268 substitution and an R411 substitution, as compared to a reference CBH I polypeptide. It is noted that the R268 and R411 numbering is made by reference to the full length T. reesei CBH I, which includes a signal sequence that is generally absent from the mature enzyme. The corresponding numbering in the mature T. reesei CBH I (see, e.g., SEQ ID NO:4) is R251 and R394, respectively.
Accordingly, the present disclosure provides variant CBH I polypeptides in which at least one of the amino acid positions corresponding to R268 and R411 of T. reesei CBH I, and optionally both the amino acid positions corresponding to R268 and R411 of T. reesei CBH I, is not an arginine.
The amino acid positions in the reference polypeptides of Table 1 that correspond to R268 and R411 in T. reesei CBH I are shown in Table 2. Amino acid positions in other CBH I polypeptides that correspond to R268 and R411 can be identified through alignment of their sequences with T. reesei CBH I using a sequence comparison algorithm. Optimal alignment of sequences for comparison can be conducted, e.g., by the local homology algorithm of Smith & Waterman, 1981, Adv. Appl. Math. 2:482-89; by the homology alignment algorithm of Needleman & Wunsch, 1970, J. Mol. Biol. 48:443-53; by the search for similarity method of Pearson & Lipman, 1988, Proc. Nat'l Acad. Sci. USA 85:2444-48, by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr, Madison, Wis.), or by visual inspection.
The R268 and/or R411 substitutions are preferably selected from (a) R268K and R411K; (b) R268K and R411A; (c) R268A and/R411K; (d) R268A and R411A; (e) R268A; (f) R268K; (g) R411A; and (h) R411K.
CBH I polypeptides belong to the glycosyl hydrolase family 7 (“GH7”). The glycosyl hydrolases of this family include endoglucanases and cellobiohydrolases (exoglucanases). The cellobiohydrolases act processively from the reducing ends of cellulose chains to generate cellobiose. Cellulases of bacterial and fungal origin characteristically have a small cellulose-binding domain (“CBD”) connected to either the N or the C terminus of the catalytic domain (“CD”) via a linker peptide (see Suumakki et al., 2000, Cellulose 7: 189-209). The CD contains the active site whereas the CBD interacts with cellulose by binding the enzyme to it (van Tilbeurgh et al., 1986, FEBS Lett. 204(2): 223-227; Tomme et al., 1988, Eur. J. Biochem. 170:575-581). The three-dimensional structure of the catalytic domain of T. reesei CBH I has been solved (Divne et al., 1994, Science 265:524-528). The CD consists of two β-sheets that pack face-to-face to form a β-sandwich. Most of the remaining amino acids in the CD are loops connecting the β-sheets. Some loops are elongated and bend around the active site, forming cellulose-binding tunnel of ( ̃50 Å). In contrast, endoglucanases have an open substrate binding cleft/groove rather than a tunnel. Typically, the catalytic residues are glutamic acids corresponding to E229 and E234 of T. reesei CBH I.
The loops characteristic of the active sites (“the active site loops”) of reference CBH I polypeptides, which are absent from GH7 family endoglucanases, as well as catalytic glutamate residues of the reference CBH I polypeptides, are shown in Table 4. The variant CBH I polypeptides of the disclosure preferably retain the catalytic glutamate residues or may include a glutamine instead at the position corresponding to E234, as for SEQ ID NO:4. In some embodiments, the variant CBH I polypeptides contain no substitutions or only conservative substitutions in the active site loops relative to the reference CBH I polypeptides from which the variants are derived.
Many CBH I polypeptides do not have a CBD, and most studies concerning the activity of cellulase domains on different substrates have been carried out with only the catalytic domains of CBH I polypeptides. Because CDs with cellobiohydrolase activity can be generated by limited proteolysis of mature CBH I by papain (see, e.g., Chen et al., 1993, Biochem. Mol. Biol. Int. 30(5):901-10), they are often referred to as “core” domains. Accordingly, a variant CBH I can include only the CD “core” of CBH I. Exemplary reference CDs comprise amino acid sequences corresponding to positions 26 to 455 of SEQ ID NO:1, positions 18 to 444 of SEQ ID NO:2, positions 26 to 455 of SEQ ID NO:3, positions 1 to 427 of SEQ ID NO:4, positions 24 to 457 of SEQ ID NO:5, positions 18 to 448 of SEQ ID NO:6, positions 27 to 460 of SEQ ID NO:7, positions 27 to 460 of SEQ ID NO:8, positions 20 to 449 of SEQ ID NO:9, positions 1 to 424 of SEQ ID NO:10, positions 18 to 447 of SEQ ID NO:11, positions 18 to 434 of SEQ ID NO:12, positions 18 to 445 of SEQ ID NO:13, positions 19 to 454 of SEQ ID NO:14, positions 19 to 443 of SEQ ID NO:15, positions 2 to 426 of SEQ ID NO:16, positions 23 to 446 of SEQ ID NO:17, positions 19 to 449 of SEQ ID NO:18, positions 23 to 446 of SEQ ID NO:19, positions 19 to 449 of SEQ ID NO:20, positions 2 to 416 of SEQ ID NO:21, positions 19 to 454 of SEQ ID NO:22, positions 19 to 447 of SEQ ID NO:23, positions 19 to 447 of SEQ ID NO:24, positions 20 to 443 of SEQ ID NO:25, positions 18 to 447 of SEQ ID NO:26, positions 19 to 442 of SEQ ID NO:27, positions 18 to 451 of SEQ ID NO:28, positions 23 to 446 of SEQ ID NO:29, positions 18 to 444 of SEQ ID NO:30, positions 18 to 451 of SEQ ID NO:31, positions 18 to 447 of SEQ ID NO:32, positions 19 to 449 of SEQ ID NO:33, positions 18 to 447 of SEQ ID NO:34, positions 26 to 459 of SEQ ID NO:35, positions 19 to 450 of SEQ ID NO:36, positions 19 to 453 of SEQ ID NO:37, positions 18 to 448 of SEQ ID NO:38, positions 19 to 443 of SEQ ID NO:39, positions 19 to 442 of SEQ ID NO:40, positions 18 to 444 of SEQ ID NO:41, positions 24 to 457 of SEQ ID NO:42, positions 18 to 449 of SEQ ID NO:43, positions 19 to 453 of SEQ ID NO:44, positions 26 to 456 of SEQ ID NO:45, positions 19 to 451 of SEQ ID NO:46, positions 18 to 443 of SEQ ID NO:47, positions 18 to 448 of SEQ ID NO:48, positions 19 to 451 of SEQ ID NO:49, positions 18 to 444 of SEQ ID NO:50, positions 2 to 419 of SEQ ID NO:51, positions 27 to 461 of SEQ ID NO:52, positions 21 to 445 of SEQ ID NO:53, positions 19 to 449 of SEQ ID NO:54, positions 19 to 448 of SEQ ID NO:55, positions 18 to 443 of SEQ ID NO:56, positions 20 to 443 of SEQ ID NO:57, positions 18 to 448 of SEQ ID NO:58, positions 18 to 447 of SEQ ID NO:59, positions 26 to 455 of SEQ ID NO:60, positions 19 to 449 of SEQ ID NO:61, positions 19 to 449 of SEQ ID NO:62, positions 26 to 460 of SEQ ID NO:63, positions 18 to 448 of SEQ ID NO:64, positions 19 to 451 of SEQ ID NO:65, positions 19 to 447 of SEQ ID NO:66, positions 1 to 424 of SEQ ID NO:67, positions 19 to 448 of SEQ ID NO:68, positions 19 to 443 of SEQ ID NO:69, positions 23 to 447 of SEQ ID NO:70, positions 17 to 448 of SEQ ID NO:71, positions 19 to 449 of SEQ ID NO:72, positions 18 to 444 of SEQ ID NO:73, positions 23 to 458 of SEQ ID NO:74, positions 20 to 452 of SEQ ID NO:75, positions 18 to 435 of SEQ ID NO:76, positions 18 to 446 of SEQ ID NO:77, positions 22 to 457 of SEQ ID NO:78, positions 18 to 448 of SEQ ID NO:79, positions 1 to 431 of SEQ ID NO:80, positions 19 to 453 of SEQ ID NO:81, positions 21 to 440 of SEQ ID NO:82, positions 19 to 442 of SEQ ID NO:83, positions 18 to 448 of SEQ ID NO:84, positions 17 to 446 of SEQ ID NO:85, positions 18 to 447 of SEQ ID NO:86, positions 18 to 443 of SEQ ID NO:87, positions 23 to 448 of SEQ ID NO:88, positions 18 to 451 of SEQ ID NO:89, positions 21 to 447 of SEQ ID NO:90, positions 18 to 444 of SEQ ID NO:91, positions 19 to 442 of SEQ ID NO:92, positions 20 to 436 of SEQ ID NO:93, positions 18 to 450 of SEQ ID NO:94, positions 22 to 453 of SEQ ID NO:95, positions 16 to 472 of SEQ ID NO:96, positions 21 to 445 of SEQ ID NO:97, positions 19 to 447 of SEQ ID NO:98, positions 19 to 450 of SEQ ID NO:99, positions 19 to 451 of SEQ ID NO:100, positions 18 to 448 of SEQ ID NO:101, positions 19 to 442 of SEQ ID NO:102, positions 20 to 457 of SEQ ID NO:103, positions 19 to 454 of SEQ ID NO:104, positions 18 to 440 of SEQ ID NO:105, positions 18 to 439 of SEQ ID NO:106, positions 27 to 460 of SEQ ID NO:107, positions 23 to 446 of SEQ ID NO:108, positions 17 to 446 of SEQ ID NO:109, positions 21 to 447 of SEQ ID NO:110, positions 19 to 447 of SEQ ID NO:111, positions 18 to 449 of SEQ ID NO:112, positions 22 to 457 of SEQ ID NO:113, positions 18 to 445 of SEQ ID NO:114, positions 18 to 448 of SEQ ID NO:115, positions 18 to 448 of SEQ ID NO:116, positions 23 to 435 of SEQ ID NO:117, positions 21 to 442 of SEQ ID NO:118, positions 23 to 435 of SEQ ID NO:119, positions 20 to 445 of SEQ ID NO:120, positions 21 to 443 of SEQ ID NO:121, positions 20 to 445 of SEQ ID NO:122, positions 23 to 443 of SEQ ID NO:123, positions 20 to 445 of SEQ ID NO:124, positions 21 to 435 of SEQ ID NO:125, positions 20 to 437 of SEQ ID NO:126, positions 21 to 442 of SEQ ID NO:127, positions 23 to 434 of SEQ ID NO:128, positions 20 to 444 of SEQ ID NO:129, positions 21 to 435 of SEQ ID NO:130, positions 20 to 445 of SEQ ID NO:131, positions 21 to 446 of SEQ ID NO:132, positions 21 to 435 of SEQ ID NO:133, positions 22 to 448 of SEQ ID NO:134, positions 23 to 433 of SEQ ID NO:135, positions 23 to 434 of SEQ ID NO:136, positions 23 to 435 of SEQ ID NO:137, positions 23 to 435 of SEQ ID NO:138, positions 20 to 445 of SEQ ID NO:139, positions 20 to 437 of SEQ ID NO:140, positions 21 to 435 of SEQ ID NO:141, positions 20 to 437 of SEQ ID NO:142, positions 21 to 435 of SEQ ID NO:143, positions 26 to 435 of SEQ ID NO:144, positions 23 to 435 of SEQ ID NO:145, positions 24 to 443 of SEQ ID NO:146, positions 20 to 445 of SEQ ID NO:147, positions 21 to 441 of SEQ ID NO:148, and positions 20 to 437 of SEQ ID NO:149.
The CBDs are particularly involved in the hydrolysis of crystalline cellulose. It has been shown that the ability of cellobiohydrolases to degrade crystalline cellulose decreases when the CBD is absent (Linder and Teeri, 1997, Journal of Biotechnol. 57:15-28). The variant CBH I polypeptides of the disclosure can further include a CBD. Exemplary CBDs comprise amino acid sequences corresponding to positions 494 to 529 of SEQ ID NO:1, positions 480 to 514 of SEQ ID NO:2, positions 494 to 529 of SEQ ID NO:3, positions 491 to 526 of SEQ ID NO:5, positions 477 to 512 of SEQ ID NO:6, positions 497 to 532 of SEQ ID NO:7, positions 504 to 539 of SEQ ID NO:8, positions 486 to 521 of SEQ ID NO:13, positions 556 to 596 of SEQ ID NO:15, positions 490 to 525 of SEQ ID NO:18, positions 495 to 530 of SEQ ID NO:20, positions 471 to 506 of SEQ ID NO:23, positions 481 to 516 of SEQ ID NO:27, positions 480 to 514 of SEQ ID NO:30, positions 495 to 529 of SEQ ID NO:35, positions 493 to 528 of SEQ ID NO:36, positions 477 to 512 of SEQ ID NO:38, positions 547 to 586 of SEQ ID NO:39, positions 475 to 510 of SEQ ID NO:40, positions 479 to 513 of SEQ ID NO:41, positions 506 to 541 of SEQ ID NO:42, positions 481 to 516 of SEQ ID NO:43, positions 503 to 537 of SEQ ID NO:45, positions 488 to 523 of SEQ ID NO:46, positions 476 to 511 of SEQ ID NO:48, positions 488 to 523 of SEQ ID NO:49, positions 479 to 513 of SEQ ID NO:50, positions 500 to 535 of SEQ ID NO:52, positions 493 to 528 of SEQ ID NO:55, positions 479 to 514 of SEQ ID NO:58, positions 494 to 529 of SEQ ID NO:60, positions 490 to 525 of SEQ ID NO:61, positions 497 to 532 of SEQ ID NO:62, positions 475 to 510 of SEQ ID NO:64, positions 477 to 512 of SEQ ID NO:65, positions 486 to 521 of SEQ ID NO:66, positions 470 to 505 of SEQ ID NO:67, positions 491 to 526 of SEQ ID NO:68, positions 476 to 511 of SEQ ID NO:69, positions 480 to 514 of SEQ ID NO:73, positions 506 to 540 of SEQ ID NO:74, positions 471 to 504 of SEQ ID NO:76, positions 501 to 536 of SEQ ID NO:78, positions 473 to 508 of SEQ ID NO:79, positions 481 to 516 of SEQ ID NO:83, positions 488 to 523 of SEQ ID NO:86, positions 475 to 510 of SEQ ID NO:92, positions 468 to 504 of SEQ ID NO:93, positions 501 to 536 of SEQ ID NO:96, positions 482 to 517 of SEQ ID NO:98, positions 481 to 516 of SEQ ID NO:99, positions 488 to 523 of SEQ ID NO:100, positions 472 to 507 of SEQ ID NO:101, positions 481 to 516 of SEQ ID NO:102, positions 471 to 505 of SEQ ID NO:105, positions 481 to 516 of SEQ ID NO:106, positions 495 to 530 of SEQ ID NO:107, positions 488 to 523 of SEQ ID NO:111, positions 478 to 513 of SEQ ID NO:112, positions 501 to 536 of SEQ ID NO:113, positions 491 to 526 of SEQ ID NO:115, and positions 503 to 538 of SEQ ID NO:116.
The CD and CBD are often connected via a linker. Exemplary linker sequences correspond to positions 456 to 493 of SEQ ID NO:1, positions 445 to 479 of SEQ ID NO:2, positions 456 to 493 of SEQ ID NO:3, positions 458 to 490 of SEQ ID NO:5, positions 449 to 476 of SEQ ID NO:6, positions 461 to 496 of SEQ ID NO:7, positions 461 to 503 of SEQ ID NO:8, positions 446 to 485 of SEQ ID NO:13, positions 444 to 555 of SEQ ID NO:15, positions 450 to 489 of SEQ ID NO:18, positions 450 to 494 of SEQ ID NO:20, positions 448 to 470 of SEQ ID NO:23, positions 443 to 480 of SEQ ID NO:27, positions 445 to 479 of SEQ ID NO:30, positions 460 to 494 of SEQ ID NO:35, positions 451 to 492 of SEQ ID NO:36, positions 449 to 476 of SEQ ID NO:38, positions 444 to 546 of SEQ ID NO:39, positions 443 to 474 of SEQ ID NO:40, positions 445 to 478 of SEQ ID NO:41, positions 458 to 505 of SEQ ID NO:42, positions 450 to 480 of SEQ ID NO:43, positions 457 to 502 of SEQ ID NO:45, positions 452 to 487 of SEQ ID NO:46, positions 449 to 475 of SEQ ID NO:48, positions 452 to 487 of SEQ ID NO:49, positions 445 to 478 of SEQ ID NO:50, positions 462 to 499 of SEQ ID NO:52, positions 449 to 492 of SEQ ID NO:55, positions 449 to 478 of SEQ ID NO:58, positions 456 to 493 of SEQ ID NO:60, positions 450 to 489 of SEQ ID NO:61, positions 450 to 496 of SEQ ID NO:62, positions 449 to 474 of SEQ ID NO:64, positions 452 to 476 of SEQ ID NO:65, positions 448 to 485 of SEQ ID NO:66, positions 425 to 469 of SEQ ID NO:67, positions 449 to 490 of SEQ ID NO:68, positions 444 to 475 of SEQ ID NO:69, positions 445 to 479 of SEQ ID NO:73, positions 459 to 505 of SEQ ID NO:74, positions 436 to 470 of SEQ ID NO:76, positions 458 to 500 of SEQ ID NO:78, positions 449 to 472 of SEQ ID NO:79, positions 443 to 480 of SEQ ID NO:83, positions 448 to 487 of SEQ ID NO:86, positions 443 to 474 of SEQ ID NO:92, positions 437 to 467 of SEQ ID NO:93, positions 473 to 500 of SEQ ID NO:96, positions 448 to 481 of SEQ ID NO:98, positions 451 to 480 of SEQ ID NO:99, positions 452 to 487 of SEQ ID NO:100, positions 449 to 471 of SEQ ID NO:101, positions 443 to 480 of SEQ ID NO:102, positions 441 to 470 of SEQ ID NO:105, positions 440 to 480 of SEQ ID NO:106, positions 461 to 494 of SEQ ID NO:107, positions 448 to 487 of SEQ ID NO:111, positions 450 to 478 of SEQ ID NO:112, positions 458 to 500 of SEQ ID NO:113, positions 449 to 490 of SEQ ID NO:115, and positions 449 to 502 of SEQ ID NO:116.
Because CBH I polypeptides are modular, the CBDs, CDs and linkers of different CBH I polypeptides, such as the exemplary CBH I polypeptides of Table 1, can be used interchangeably. However, in a preferred embodiment, the CBDs, CDs and linkers of a variant CBH I of the disclosure originate from the same polypeptide.
The variant CBH I polypeptides of the disclosure preferably have at least a two-fold reduction of product inhibition, such that cellobiose has an IC50 towards the variant CBH I that is at least 2-fold the IC50 of the corresponding reference CBH I, e.g., CBH I lacking the R268 substitution and/or R411 substitution. More preferably the IC50 of cellobiose towards the variant CBH I is at least 3-fold, at least 5-fold, at least 8-fold, at least 10-fold, at least 12-fold or at least 15-fold the IC50 of the corresponding reference CBH I. In specific embodiments the IC50 of cellobiose towards the variant CBH I is ranges from 2-fold to 15-fold, from 2-fold to 10-fold, from 3-fold to 10-fold, from 5-fold to 12-fold, from 4-fold to 12-fold, from 5-fold to 10-fold, from 5-fold to 12-fold, from 2-fold to 8-fold, or from 8-fold to 20-fold the IC50 of the corresponding reference CBH I. The IC50 can be determined in a phosphoric acid swollen cellulose (“PASC”) assay (Du et al., 2010, Applied Biochemistry and Biotechnology 161:313-317) or a methylumbelliferyl lactoside (“MUL”) assay (van Tilbeurgh and Claeyssens, 1985, FEBS Letts. 187(2):283-288), as exemplified in the Examples below.
The variant CBH I polypeptides of the disclosure preferably have a cellobiohydrolase activity that is at least 30% the cellobiohydrolase activity of the corresponding reference CBH I, e.g., CBH I lacking the R268 substitution and/or R411 substitution. More preferably, the cellobiohydrolase activity of the variant CBH I is at least 40%, at least 50%, at least 60% or at least 70% the cellobiohydrolase activity of the corresponding reference CBH I. In specific embodiments the IC50 cellobiohydrolase activity of the variant CBH I is ranges from 30% to 80%, from 40% to 70%, 30% to 60%, from 50% to 80% or from 60% to 80% of the cellobiohydrolase activity of the corresponding reference CBH I. Assays for cellobiohydrolase activity are described, for example, in Becker et al., 2011, Biochem J. 356:19-30 and Mitsuishi et al., 1990, FEBS Letts. 275:135-138, each of which is expressly incorporated by reference herein. The ability of CBH Ito hydrolyze isolated soluble and insoluble substrates can also be measured using assays described in Srisodsuk et al., 1997, J. Biotech. 57:4957 and Nidetzky and Claeyssens, 1994, Biotech. Bioeng. 44:961-966. Substrates useful for assaying cellobiohydrolase activity include crystalline cellulose, filter paper, phosphoric acid swollen cellulose, cellooligosaccharides, methylumbelliferyl lactoside, methylumbelliferyl cellobioside, orthonitrophenyl lactoside, paranitrophenyl lactoside, orthonitrophenyl cellobioside, paranitrophenyl cellobioside. Cellobiohydrolase activity can be measured in an assay utilizing PASC as the substrate and a calcofluor white detection method (Du et al., 2010, Applied Biochemistry and Biotechnology 161:313-317). PASC can be prepared as described by Walseth, 1952, TAPPI 35:228-235 and Wood, 1971, Biochem. J. 121:353-362.
Other than said R268 and/or R411 substitution, the variant CBH I polypeptides of the disclosure preferably:

    
    
        comprise an amino acid sequence having at least 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more, or complete (100%) sequence identity to a CD of a reference CBH I exemplified in Table 1 (i.e., a CD comprising an amino acid sequence corresponding to positions 26 to 455 of SEQ ID NO:1, positions 18 to 444 of SEQ ID NO:2, positions 26 to 455 of SEQ ID NO:3, positions 1 to 427 of SEQ ID NO:4, positions 24 to 457 of SEQ ID NO:5, positions 18 to 448 of SEQ ID NO:6, positions 27 to 460 of SEQ ID NO:7, positions 27 to 460 of SEQ ID NO:8, positions 20 to 449 of SEQ ID NO:9, positions 1 to 424 of SEQ ID NO:10, positions 18 to 447 of SEQ ID NO:11, positions 18 to 434 of SEQ ID NO:12, positions 18 to 445 of SEQ ID NO:13, positions 19 to 454 of SEQ ID NO:14, positions 19 to 443 of SEQ ID NO:15, positions 2 to 426 of SEQ ID NO:16, positions 23 to 446 of SEQ ID NO:17, positions 19 to 449 of SEQ ID NO:18, positions 23 to 446 of SEQ ID NO:19, positions 19 to 449 of SEQ ID NO:20, positions 2 to 416 of SEQ ID NO:21, positions 19 to 454 of SEQ ID NO:22, positions 19 to 447 of SEQ ID NO:23, positions 19 to 447 of SEQ ID NO:24, positions 20 to 443 of SEQ ID NO:25, positions 18 to 447 of SEQ ID NO:26, positions 19 to 442 of SEQ ID NO:27, positions 18 to 451 of SEQ ID NO:28, positions 23 to 446 of SEQ ID NO:29, positions 18 to 444 of SEQ ID NO:30, positions 18 to 451 of SEQ ID NO:31, positions 18 to 447 of SEQ ID NO:32, positions 19 to 449 of SEQ ID NO:33, positions 18 to 447 of SEQ ID NO:34, positions 26 to 459 of SEQ ID NO:35, positions 19 to 450 of SEQ ID NO:36, positions 19 to 453 of SEQ ID NO:37, positions 18 to 448 of SEQ ID NO:38, positions 19 to 443 of SEQ ID NO:39, positions 19 to 442 of SEQ ID NO:40, positions 18 to 444 of SEQ ID NO:41, positions 24 to 457 of SEQ ID NO:42, positions 18 to 449 of SEQ ID NO:43, positions 19 to 453 of SEQ ID NO:44, positions 26 to 456 of SEQ ID NO:45, positions 19 to 451 of SEQ ID NO:46, positions 18 to 443 of SEQ ID NO:47, positions 18 to 448 of SEQ ID NO:48, positions 19 to 451 of SEQ ID NO:49, positions 18 to 444 of SEQ ID NO:50, positions 2 to 419 of SEQ ID NO:51, positions 27 to 461 of SEQ ID NO:52, positions 21 to 445 of SEQ ID NO:53, positions 19 to 449 of SEQ ID NO:54, positions 19 to 448 of SEQ ID NO:55, positions 18 to 443 of SEQ ID NO:56, positions 20 to 443 of SEQ ID NO:57, positions 18 to 448 of SEQ ID NO:58, positions 18 to 447 of SEQ ID NO:59, positions 26 to 455 of SEQ ID NO:60, positions 19 to 449 of SEQ ID NO:61, positions 19 to 449 of SEQ ID NO:62, positions 26 to 460 of SEQ ID NO:63, positions 18 to 448 of SEQ ID NO:64, positions 19 to 451 of SEQ ID NO:65, positions 19 to 447 of SEQ ID NO:66, positions 1 to 424 of SEQ ID NO:67, positions 19 to 448 of SEQ ID NO:68, positions 19 to 443 of SEQ ID NO:69, positions 23 to 447 of SEQ ID NO:70, positions 17 to 448 of SEQ ID NO:71, positions 19 to 449 of SEQ ID NO:72, positions 18 to 444 of SEQ ID NO:73, positions 23 to 458 of SEQ ID NO:74, positions 20 to 452 of SEQ ID NO:75, positions 18 to 435 of SEQ ID NO:76, positions 18 to 446 of SEQ ID NO:77, positions 22 to 457 of SEQ ID NO:78, positions 18 to 448 of SEQ ID NO:79, positions 1 to 431 of SEQ ID NO:80, positions 19 to 453 of SEQ ID NO:81, positions 21 to 440 of SEQ ID NO:82, positions 19 to 442 of SEQ ID NO:83, positions 18 to 448 of SEQ ID NO:84, positions 17 to 446 of SEQ ID NO:85, positions 18 to 447 of SEQ ID NO:86, positions 18 to 443 of SEQ ID NO:87, positions 23 to 448 of SEQ ID NO:88, positions 18 to 451 of SEQ ID NO:89, positions 21 to 447 of SEQ ID NO:90, positions 18 to 444 of SEQ ID NO:91, positions 19 to 442 of SEQ ID NO:92, positions 20 to 436 of SEQ ID NO:93, positions 18 to 450 of SEQ ID NO:94, positions 22 to 453 of SEQ ID NO:95, positions 16 to 472 of SEQ ID NO:96, positions 21 to 445 of SEQ ID NO:97, positions 19 to 447 of SEQ ID NO:98, positions 19 to 450 of SEQ ID NO:99, positions 19 to 451 of SEQ ID NO:100, positions 18 to 448 of SEQ ID NO:101, positions 19 to 442 of SEQ ID NO:102, positions 20 to 457 of SEQ ID NO:103, positions 19 to 454 of SEQ ID NO:104, positions 18 to 440 of SEQ ID NO:105, positions 18 to 439 of SEQ ID NO:106, positions 27 to 460 of SEQ ID NO:107, positions 23 to 446 of SEQ ID NO:108, positions 17 to 446 of SEQ ID NO:109, positions 21 to 447 of SEQ ID NO:110, positions 19 to 447 of SEQ ID NO:111, positions 18 to 449 of SEQ ID NO:112, positions 22 to 457 of SEQ ID NO:113, positions 18 to 445 of SEQ ID NO:114, positions 18 to 448 of SEQ ID NO:115, positions 18 to 448 of SEQ ID NO:116, positions 23 to 435 of SEQ ID NO:117, positions 21 to 442 of SEQ ID NO:118, positions 23 to 435 of SEQ ID NO:119, positions 20 to 445 of SEQ ID NO:120, positions 21 to 443 of SEQ ID NO:121, positions 20 to 445 of SEQ ID NO:122, positions 23 to 443 of SEQ ID NO:123, positions 20 to 445 of SEQ ID NO:124, positions 21 to 435 of SEQ ID NO:125, positions 20 to 437 of SEQ ID NO:126, positions 21 to 442 of SEQ ID NO:127, positions 23 to 434 of SEQ ID NO:128, positions 20 to 444 of SEQ ID NO:129, positions 21 to 435 of SEQ ID NO:130, positions 20 to 445 of SEQ ID NO:131, positions 21 to 446 of SEQ ID NO:132, positions 21 to 435 of SEQ ID NO:133, positions 22 to 448 of SEQ ID NO:134, positions 23 to 433 of SEQ ID NO:135, positions 23 to 434 of SEQ ID NO:136, positions 23 to 435 of SEQ ID NO:137, positions 23 to 435 of SEQ ID NO:138, positions 20 to 445 of SEQ ID NO:139, positions 20 to 437 of SEQ ID NO:140, positions 21 to 435 of SEQ ID NO:141, positions 20 to 437 of SEQ ID NO:142, positions 21 to 435 of SEQ ID NO:143, positions 26 to 435 of SEQ ID NO:144, positions 23 to 435 of SEQ ID NO:145, positions 24 to 443 of SEQ ID NO:146, positions 20 to 445 of SEQ ID NO:147, positions 21 to 441 of SEQ ID NO:148, and positions 20 to 437 of SEQ ID NO:149 (preferably the CD corresponding to positions 26-455 of SEQ ID NO: 1 or 18-444 of SEQ ID NO:2); and/or
        comprise an amino acid sequence having at least 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more, or complete (100%) sequence identity to a mature polypeptide of a reference CBH I exemplified in Table 1 (i.e., a mature protein comprising an amino acid sequence corresponding to positions 26 to 529 of SEQ ID NO:1, positions 18 to 514 of SEQ ID NO:2, positions 26 to 529 of SEQ ID NO:3, positions 1 to 427 of SEQ ID NO:4, positions 24 to 526 of SEQ ID NO:5, positions 18 to 512 of SEQ ID NO:6, positions 27 to 532 of SEQ ID NO:7, positions 27 to 539 of SEQ ID NO:8, positions 20 to 449 of SEQ ID NO:9, positions 1 to 424 of SEQ ID NO:10, positions 18 to 447 of SEQ ID NO:11, positions 18 to 434 of SEQ ID NO:12, positions 18 to 521 of SEQ ID NO:13, positions 19 to 454 of SEQ ID NO:14, positions 19 to 596 of SEQ ID NO:15, positions 2 to 426 of SEQ ID NO:16, positions 23 to 446 of SEQ ID NO:17, positions 19 to 525 of SEQ ID NO:18, positions 23 to 446 of SEQ ID NO:19, positions 19 to 530 of SEQ ID NO:20, positions 2 to 416 of SEQ ID NO:21, positions 19 to 454 of SEQ ID NO:22, positions 19 to 506 of SEQ ID NO:23, positions 19 to 447 of SEQ ID NO:24, positions 20 to 443 of SEQ ID NO:25, positions 18 to 447 of SEQ ID NO:26, positions 19 to 516 of SEQ ID NO:27, positions 18 to 451 of SEQ ID NO:28, positions 23 to 446 of SEQ ID NO:29, positions 18 to 514 of SEQ ID NO:30, positions 18 to 451 of SEQ ID NO:31, positions 18 to 447 of SEQ ID NO:32, positions 19 to 449 of SEQ ID NO:33, positions 18 to 447 of SEQ ID NO:34, positions 26 to 529 of SEQ ID NO:35, positions 19 to 528 of SEQ ID NO:36, positions 19 to 453 of SEQ ID NO:37, positions 18 to 512 of SEQ ID NO:38, positions 19 to 586 of SEQ ID NO:39, positions 19 to 510 of SEQ ID NO:40, positions 18 to 513 of SEQ ID NO:41, positions 24 to 541 of SEQ ID NO:42, positions 18 to 516 of SEQ ID NO:43, positions 19 to 453 of SEQ ID NO:44, positions 26 to 537 of SEQ ID NO:45, positions 19 to 523 of SEQ ID NO:46, positions 18 to 443 of SEQ ID NO:47, positions 18 to 511 of SEQ ID NO:48, positions 19 to 523 of SEQ ID NO:49, positions 18 to 513 of SEQ ID NO:50, positions 2 to 419 of SEQ ID NO:51, positions 27 to 535 of SEQ ID NO:52, positions 21 to 445 of SEQ ID NO:53, positions 19 to 449 of SEQ ID NO:54, positions 19 to 528 of SEQ ID NO:55, positions 18 to 443 of SEQ ID NO:56, positions 20 to 443 of SEQ ID NO:57, positions 18 to 514 of SEQ ID NO:58, positions 18 to 447 of SEQ ID NO:59, positions 26 to 529 of SEQ ID NO:60, positions 19 to 525 of SEQ ID NO:61, positions 19 to 532 of SEQ ID NO:62, positions 26 to 460 of SEQ ID NO:63, positions 18 to 510 of SEQ ID NO:64, positions 19 to 512 of SEQ ID NO:65, positions 19 to 521 of SEQ ID NO:66, positions 1 to 505 of SEQ ID NO:67, positions 19 to 526 of SEQ ID NO:68, positions 19 to 511 of SEQ ID NO:69, positions 23 to 447 of SEQ ID NO:70, positions 17 to 448 of SEQ ID NO:71, positions 19 to 449 of SEQ ID NO:72, positions 18 to 514 of SEQ ID NO:73, positions 23 to 540 of SEQ ID NO:74, positions 20 to 452 of SEQ ID NO:75, positions 18 to 504 of SEQ ID NO:76, positions 18 to 446 of SEQ ID NO:77, positions 22 to 536 of SEQ ID NO:78, positions 18 to 508 of SEQ ID NO:79, positions 1 to 431 of SEQ ID NO:80, positions 19 to 453 of SEQ ID NO:81, positions 21 to 440 of SEQ ID NO:82, positions 19 to 516 of SEQ ID NO:83, positions 18 to 448 of SEQ ID NO:84, positions 17 to 446 of SEQ ID NO:85, positions 18 to 523 of SEQ ID NO:86, positions 18 to 443 of SEQ ID NO:87, positions 23 to 448 of SEQ ID NO:88, positions 18 to 451 of SEQ ID NO:89, positions 21 to 447 of SEQ ID NO:90, positions 18 to 444 of SEQ ID NO:91, positions 19 to 510 of SEQ ID NO:92, positions 20 to 504 of SEQ ID NO:93, positions 18 to 450 of SEQ ID NO:94, positions 22 to 453 of SEQ ID NO:95, positions 16 to 536 of SEQ ID NO:96, positions 21 to 445 of SEQ ID NO:97, positions 19 to 517 of SEQ ID NO:98, positions 19 to 516 of SEQ ID NO:99, positions 19 to 523 of SEQ ID NO:100, positions 18 to 507 of SEQ ID NO:101, positions 19 to 516 of SEQ ID NO:102, positions 20 to 457 of SEQ ID NO:103, positions 19 to 454 of SEQ ID NO:104, positions 18 to 505 of SEQ ID NO:105, positions 18 to 516 of SEQ ID NO:106, positions 27 to 530 of SEQ ID NO:107, positions 23 to 446 of SEQ ID NO:108, positions 17 to 446 of SEQ ID NO:109, positions 21 to 447 of SEQ ID NO:110, positions 19 to 523 of SEQ ID NO:111, positions 18 to 513 of SEQ ID NO:112, positions 22 to 536 of SEQ ID NO:113, positions 18 to 445 of SEQ ID NO:114, positions 18 to 526 of SEQ ID NO:115, positions 18 to 538 of SEQ ID NO:116, positions 23 to 435 of SEQ ID NO:117, positions 21 to 442 of SEQ ID NO:118, positions 23 to 435 of SEQ ID NO:119, positions 20 to 445 of SEQ ID NO:120, positions 21 to 443 of SEQ ID NO:121, positions 20 to 445 of SEQ ID NO:122, positions 23 to 443 of SEQ ID NO:123, positions 20 to 445 of SEQ ID NO:124, positions 21 to 435 of SEQ ID NO:125, positions 20 to 437 of SEQ ID NO:126, positions 21 to 442 of SEQ ID NO:127, positions 23 to 434 of SEQ ID NO:128, positions 20 to 444 of SEQ ID NO:129, positions 21 to 435 of SEQ ID NO:130, positions 20 to 445 of SEQ ID NO:131, positions 21 to 446 of SEQ ID NO:132, positions 21 to 435 of SEQ ID NO:133, positions 22 to 448 of SEQ ID NO:134, positions 23 to 433 of SEQ ID NO:135, positions 23 to 434 of SEQ ID NO:136, positions 23 to 435 of SEQ ID NO:137, positions 23 to 435 of SEQ ID NO:138, positions 20 to 445, of SEQ ID NO:139, positions 20 to 437 of SEQ ID NO:140, positions 21 to 435 of SEQ ID NO:141, positions 20 to 437 of SEQ ID NO:142, positions 21 to 435 of SEQ ID NO:143, positions 26 to 435 of SEQ ID NO:144, positions 23 to 435 of SEQ ID NO:145, positions 24 to 443 of SEQ ID NO:146, positions 20 to 445 of SEQ ID NO:147, positions 21 to 441 of SEQ ID NO:148, and positions 20 to 437 of SEQ ID NO:149, preferably the mature polypeptide corresponding to positions 26-529 of SEQ ID NO:1 or 18-514 of SEQ ID NO:2).
    
    


An example of an algorithm that is suitable for determining sequence similarity is the BLAST algorithm, which is described in Altschul et al., 1990, J. Mol. Biol. 215:403-410. Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information. This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence that either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. These initial neighborhood word hits act as starting points to find longer HSPs containing them. The word hits are expanded in both directions along each of the two sequences being compared for as far as the cumulative alignment score can be increased. Extension of the word hits is stopped when: the cumulative alignment score falls off by the quantity X from a maximum achieved value; the cumulative score goes to zero or below; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLAST program uses as defaults a word length (W) of 11, the BLOSUM62 scoring matrix (see Henikoff & Henikoff, 1992, Proc. Nat'l. Acad. Sci. USA 89:10915-10919) alignments (B) of 50, expectation (E) of 10, M′S, N′-4, and a comparison of both strands.
Most CBH I polypeptides are secreted and are therefore expressed with a signal sequence that is cleaved upon secretion of the polypeptide from the cell. Accordingly, in certain aspects, the variant CBH I polypeptides of the disclosure further include a signal sequence. Exemplary signal sequences comprise amino acid sequences corresponding to positions 1 to 25 of SEQ ID NO:1, positions 1 to 17 of SEQ ID NO:2, positions 1 to 25 of SEQ ID NO:3, positions 1 to 23 of SEQ ID NO:5, positions 1 to 17 of SEQ ID NO:6, positions 1 to 26 of SEQ ID NO:7, positions 1 to 27 of SEQ ID NO:8, positions 1 to 19 of SEQ ID NO:9, positions 1 to 17 of SEQ ID NO:11, positions 1 to 17 of SEQ ID NO:12, positions 1 to 17 of SEQ ID NO:13, positions 1 to 18 of SEQ ID NO:14, positions 1 to 18 of SEQ ID NO:15, positions 1 to 22 of SEQ ID NO:17, positions 1 to 18 of SEQ ID NO:18, positions 1 to 22 of SEQ ID NO:19, positions 1 to 18 of SEQ ID NO:20, positions 1 to 18 of SEQ ID NO:22, positions 1 to 18 of SEQ ID NO:23, positions 1 to 18 of SEQ ID NO:24, positions 1 to 19 of SEQ ID NO:25, positions 1 to 17 of SEQ ID NO:26, positions 1 to 18 of SEQ ID NO:27, positions 1 to 17 of SEQ ID NO:28, positions 1 to 22 of SEQ ID NO:29, positions 1 to 18 of SEQ ID NO:30, positions 1 to 17 of SEQ ID NO:31, positions 1 to 17 of SEQ ID NO:32, positions 1 to 18 of SEQ ID NO:33, positions 1 to 17 of SEQ ID NO:34, positions 1 to 25 of SEQ ID NO:35, positions 1 to 18 of SEQ ID NO:36, positions 1 to 18 of SEQ ID NO:37, positions 1 to 17 of SEQ ID NO:38, positions 1 to 18 of SEQ ID NO:39, positions 1 to 18 of SEQ ID NO:40, positions 1 to 17 of SEQ ID NO:41, positions 1 to 23 of SEQ ID NO:42, positions 1 to 17 of SEQ ID NO:43, positions 1 to 18 of SEQ ID NO:44, positions 1 to 25 of SEQ ID NO:45, positions 1 to 18 of SEQ ID NO:46, positions 1 to 17 of SEQ ID NO:47, positions 1 to 17 of SEQ ID NO:48, positions 1 to 18 of SEQ ID NO:49, positions 1 to 17 of SEQ ID NO:50, positions 1 to 26 of SEQ ID NO:52, positions 1 to 20 of SEQ ID NO:53, positions 1 to 18 of SEQ ID NO:54, positions 1 to 18 of SEQ ID NO:55, positions 1 to 17 of SEQ ID NO:56, positions 1 to 19 of SEQ ID NO:57, positions 1 to 17 of SEQ ID NO:58, positions 1 to 17 of SEQ ID NO:59, positions 1 to 25 of SEQ ID NO:60, positions 1 to 18 of SEQ ID NO:61, positions 1 to 18 of SEQ ID NO:62, positions 1 to 25 of SEQ ID NO:63, positions 1 to 17 of SEQ ID NO:64, positions 1 to 18 of SEQ ID NO:65, positions 1 to 18 of SEQ ID NO:66, positions 1 to 18 of SEQ ID NO:68, positions 1 to 18 of SEQ ID NO:69, positions 1 to 23 of SEQ ID NO:70, positions 1 to 17 of SEQ ID NO:71, positions 1 to 18 of SEQ ID NO:72, positions 1 to 17 of SEQ ID NO:73, positions 1 to 22 of SEQ ID NO:74, positions 1 to 19 of SEQ ID NO:75, positions 1 to 17 of SEQ ID NO:76, positions 1 to 17 of SEQ ID NO:77, positions 1 to 21 of SEQ ID NO:78, positions 1 to 18 of SEQ ID NO:79, positions 1 to 18 of SEQ ID NO:81, positions 1 to 20 of SEQ ID NO:82, positions 1 to 18 of SEQ ID NO:83, positions 1 to 17 of SEQ ID NO:84, positions 1 to 16 of SEQ ID NO:85, positions 1 to 17 of SEQ ID NO:86, positions 1 to 17 of SEQ ID NO:87, positions 1 to 22 of SEQ ID NO:88, positions 1 to 17 of SEQ ID NO:89, positions 1 to 20 of SEQ ID NO:90, positions 1 to 17 of SEQ ID NO:91, positions 1 to 18 of SEQ ID NO:92, positions 1 to 19 of SEQ ID NO:93, positions 1 to 17 of SEQ ID NO:94, positions 1 to 21 of SEQ ID NO:95, positions 1 to 15 of SEQ ID NO:96, positions 1 to 20 of SEQ ID NO:97, positions 1 to 18 of SEQ ID NO:98, positions 1 to 18 of SEQ ID NO:99, positions 1 to 18 of SEQ ID NO:100, positions 1 to 17 of SEQ ID NO:101, positions 1 to 18 of SEQ ID NO:102, positions 1 to 19 of SEQ ID NO:103, positions 1 to 18 of SEQ ID NO:104, positions 1 to 17 of SEQ ID NO:105, positions 1 to 17 of SEQ ID NO:106, positions 1 to 26 of SEQ ID NO:107, positions 1 to 22 of SEQ ID NO:108, positions 1 to 16 of SEQ ID NO:109, positions 1 to 20 of SEQ ID NO:110, positions 1 to 18 of SEQ ID NO:111, positions 1 to 17 of SEQ ID NO:112, positions 1 to 21 of SEQ ID NO:113, positions 1 to 17 of SEQ ID NO:114, positions 1 to 17 of SEQ ID NO:115, positions 1 to 18 of SEQ ID NO:116, positions 1 to 22 of SEQ ID NO:117, positions 1 to 20 of SEQ ID NO:118, positions 1 to 22 of SEQ ID NO:119, positions 1 to 19 of SEQ ID NO:120, positions 1 to 20 of SEQ ID NO:121, positions 1 to 19 of SEQ ID NO:122, positions 1 to 22 of SEQ ID NO:123, positions 1 to 19 of SEQ ID NO:124, positions 1 to 20 of SEQ ID NO:125, positions 1 to 19 of SEQ ID NO:126, positions 1 to 21 of SEQ ID NO:127, positions 1 to 22 of SEQ ID NO:128, positions 1 to 19 of SEQ ID NO:129, positions 1 to 20 of SEQ ID NO:130, positions 1 to 19 of SEQ ID NO:131, positions 1 to 20 of SEQ ID NO:132, positions 1 to 20 of SEQ ID NO:133, positions 1 to 21 of SEQ ID NO:134, positions 1 to 22 of SEQ ID NO:135, positions 1 to 22 of SEQ ID NO:136, positions 1 to 22 of SEQ ID NO:137, positions 1 to 22 of SEQ ID NO:138, positions 1 to 19 of SEQ ID NO:139, positions 1 to 19 of SEQ ID NO:140, positions 1 to 20 of SEQ ID NO:141, positions 1 to 19 of SEQ ID NO:142, positions 1 to 20 of SEQ ID NO:143, positions 1 to 25 of SEQ ID NO:144, positions 1 to 22 of SEQ ID NO:145, positions 1 to 23 of SEQ ID NO:146, positions 1 to 19 of SEQ ID NO:147, positions 1 to 20 of SEQ ID NO:148, and positions 1 to 19 of SEQ ID NO:149.
Recombinant Expression of Variant CBH I Polypeptides
Cell Culture Systems
The disclosure also provides recombinant cells engineered to express variant CBH I polypeptides. Suitably, the variant CBH I polypeptide is encoded by a nucleic acid operably linked to a promoter.
Where recombinant expression in a filamentous fungal host is desired, the promoter can be a filamentous fungal promoter. The nucleic acids can be, for example, under the control of heterologous promoters. The variant CBH I polypeptides can also be expressed under the control of constitutive or inducible promoters. Examples of promoters that can be used include, but are not limited to, a cellulase promoter, a xylanase promoter, the 1818 promoter (previously identified as a highly expressed protein by EST mapping Trichoderma). For example, the promoter can suitably be a cellobiohydrolase, endoglucanase, or β-glucosidase promoter. A particularly suitable promoter can be, for example, a T. reesei cellobiohydrolase, endoglucanase, or β-glucosidase promoter. Non-limiting examples of promoters include a cbh1, cbh2, egl1, egl2, egl3, egl4, egl5, pki1, gpd1, xyn1, or xyn2 promoter.
Suitable host cells include cells of any microorganism (e.g., cells of a bacterium, a protist, an alga, a fungus (e.g., a yeast or filamentous fungus), or other microbe), and are preferably cells of a bacterium, a yeast, or a filamentous fungus.
Suitable host cells of the bacterial genera include, but are not limited to, cells of Escherichia, Bacillus, Lactobacillus, Pseudomonas, and Streptomyces. Suitable cells of bacterial species include, but are not limited to, cells of Escherichia coli, Bacillus subtilis, Bacillus licheniformis, Lactobacillus brevis, Pseudomonas aeruginosa, and Streptomyces lividans. 
Suitable host cells of the genera of yeast include, but are not limited to, cells of Saccharomyces, Schizosaccharomyces, Candida, Hansenula, Pichia, Kluyveromyces, and Phaffia. Suitable cells of yeast species include, but are not limited to, cells of Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida albicans, Hansenula polymorpha, Pichia pastoris, P. canadensis, Kluyveromyces marxianus, and Phaffia rhodozyma. 
Suitable host cells of filamentous fungi include all filamentous forms of the subdivision Eumycotina. Suitable cells of filamentous fungal genera include, but are not limited to, cells of Acremonium, Aspergillus, Aureobasidium, Bjerkandera, Ceriporiopsis, Chrysoporium, Coprinus, Coriolus, Corynascus, Chaetomium, Cryptococcus, Filobasidium, Fusarium, Gibberella, Humicola, Hypocrea, Magnaporthe, Mucor, Myceliophthora, Mucor, Neocallimastix, Neurospora, Paecilomyces, Penicillium, Phanerochaete, Phlebia, Piromyces, Pleurotus, Scytaldium, Schizophyllum, Sporotrichum, Talaromyces, Thermoascus, Thielavia, Tolypocladium, Trametes, and Trichoderma. More preferably, the recombinant cell is a Trichoderma sp. (e.g., Trichoderma reesei), Penicillium sp., Humicola sp. (e.g., Humicola insolens); Aspergillus sp. (e.g., Aspergillus niger), Chrysosporium sp., Fusarium sp., or Hypocrea sp. Suitable cells can also include cells of various anamorph and teleomorph forms of these filamentous fungal genera.
Suitable cells of filamentous fungal species include, but are not limited to, cells of Aspergillus awamori, Aspergillus fumigatus, Aspergillus foetidus, Aspergillus japonicus, Aspergillus nidulans, Aspergillus niger, Aspergillus oryzae, Chrysosporium lucknowense, Fusarium bactridioides, Fusarium cerealis, Fusarium crookwellense, Fusarium culmorum, Fusarium graminearum, Fusarium graminum, Fusarium heterosporum, Fusarium negundi, Fusarium oxysporum, Fusarium reticulatum, Fusarium roseum, Fusarium sambucinum, Fusarium sarcochroum, Fusarium sporotrichioides, Fusarium sulphureum, Fusarium torulosum, Fusarium trichothecioides, Fusarium venenatum, Bjerkandera adusta, Ceriporiopsis aneirina, Ceriporiopsis aneirina, Ceriporiopsis caregiea, Ceriporiopsis gilvescens, Ceriporiopsis pannocinta, Ceriporiopsis rivulosa, Ceriporiopsis subrufa, Ceriporiopsis subvermispora, Coprinus cinereus, Coriolus hirsutus, Humicola insolens, Humicola lanuginosa, Mucor miehei, Myceliophthora thermophila, Neurospora crassa, Neurospora intermedia, Penicillium purpurogenum, Penicillium canescens, Penicillium solitum, Penicillium funiculosum, Phanerochaete chrysosporium, Phlebia radiate, Pleurotus eryngii, Talaromyces flavus, Thielavia terrestris, Trametes villosa, Trametes versicolor, Trichoderma harzianum, Trichoderma koningii, Trichoderma longibrachiatum, Trichoderma reesei, and Trichoderma viride. 
The engineered host cells can be cultured in conventional nutrient media modified as appropriate for activating promoters, selecting transformants, or amplifying the nucleic acid sequence encoding the variant CBH I polypeptide. Culture conditions, such as temperature, pH and the like, are those previously used with the host cell selected for expression, and will be apparent to those skilled in the art. As noted, many references are available for the culture and production of many cells, including cells of bacterial and fungal origin. Cell culture media in general are set forth in Atlas and Parks (eds.), 1993, The Handbook of Microbiological Media, CRC Press, Boca Raton, Fla., which is incorporated herein by reference. For recombinant expression in filamentous fungal cells, the cells are cultured in a standard medium containing physiological salts and nutrients, such as described in Pourquie et al., 1988, Biochemistry and Genetics of Cellulose Degradation, eds. Aubert, et al., Academic Press, pp. 71-86; and Ilmen et al., 1997, Appl. Environ. Microbiol. 63:1298-1306. Culture conditions are also standard, e.g., cultures are incubated at 28° C. in shaker cultures or fermenters until desired levels of variant CBH I expression are achieved. Preferred culture conditions for a given filamentous fungus may be found in the scientific literature and/or from the source of the fungi such as the American Type Culture Collection (ATCC). After fungal growth has been established, the cells are exposed to conditions effective to cause or permit the expression of a variant CBH I.
In cases where a variant CBH I coding sequence is under the control of an inducible promoter, the inducing agent, e.g., a sugar, metal salt or antibiotics, is added to the medium at a concentration effective to induce variant CBH I expression.
In one embodiment, the recombinant cell is an Aspergillus niger, which is a useful strain for obtaining overexpressed polypeptide. For example A. niger var. awamori dgr246 is known to product elevated amounts of secreted cellulases (Goedegebuur et al., 2002, Curr. Genet. 41:89-98). Other strains of Aspergillus niger var awamori such as GCDAP3, GCDAP4 and GAP3-4 are known (Ward et al., 1993, Appl. Microbiol. Biotechnol. 39:738-743).
In another embodiment, the recombinant cell is a Trichoderma reesei, which is a useful strain for obtaining overexpressed polypeptide. For example, RL-P37, described by Sheir-Neiss et al., 1984, Appl. Microbiol. Biotechnol. 20:46-53, is known to secrete elevated amounts of cellulase enzymes. Functional equivalents of RL-P37 include Trichoderma reesei strain RUT-C30 (ATCC No. 56765) and strain QM9414 (ATCC No. 26921). It is contemplated that these strains would also be useful in overexpressing variant CBH I polypeptides.
Cells expressing the variant CBH I polypeptides of the disclosure can be grown under batch, fed-batch or continuous fermentations conditions. Classical batch fermentation is a closed system, wherein the compositions of the medium is set at the beginning of the fermentation and is not subject to artificial alternations during the fermentation. A variation of the batch system is a fed-batch fermentation in which the substrate is added in increments as the fermentation progresses. Fed-batch systems are useful when catabolite repression is likely to inhibit the metabolism of the cells and where it is desirable to have limited amounts of substrate in the medium. Batch and fed-batch fermentations are common and well known in the art. Continuous fermentation is an open system where a defined fermentation medium is added continuously to a bioreactor and an equal amount of conditioned medium is removed simultaneously for processing. Continuous fermentation generally maintains the cultures at a constant high density where cells are primarily in log phase growth. Continuous fermentation systems strive to maintain steady state growth conditions. Methods for modulating nutrients and growth factors for continuous fermentation processes as well as techniques for maximizing the rate of product formation are well known in the art of industrial microbiology.
Recombinant Expression in Plants
The disclosure provides transgenic plants and seeds that recombinantly express a variant CBH I polypeptide. The disclosure also provides plant products, e.g., oils, seeds, leaves, extracts and the like, comprising a variant CBH I polypeptide.
The transgenic plant can be dicotyledonous (a dicot) or monocotyledonous (a monocot). The disclosure also provides methods of making and using these transgenic plants and seeds. The transgenic plant or plant cell expressing a variant CBH I can be constructed in accordance with any method known in the art. See, for example, U.S. Pat. No. 6,309,872. T. reesei CBH I has been successfully expressed in transgenic tobacco (Nicotiana tabaccum) and potato (Solanum tuberosum). See Hooker et al., 2000, in Glycosyl Hydrolases for Biomass Conversion, ACS Symposium Series, Vol. 769, Chapter 4, pp. 55-90.
In a particular aspect, the present disclosure provides for the expression of CBH I variants in transgenic plants or plant organs and methods for the production thereof. DNA expression constructs are provided for the transformation of plants with a nucleic acid encoding the variant CBH I polypeptide, preferably under the control of regulatory sequences which are capable of directing expression of the variant CBH I polypeptide. These regulatory sequences include sequences capable of directing transcription in plants, either constitutively, or in stage and/or tissue specific manners.
The expression of variant CBH I polypeptides in plants can be achieved by a variety of means. Specifically, for example, technologies are available for transforming a large number of plant species, including dicotyledonous species (e.g., tobacco, potato, tomato, Petunia, Brassica) and monocot species. Additionally, for example, strategies for the expression of foreign genes in plants are available. Additionally still, regulatory sequences from plant genes have been identified that are serviceable for the construction of chimeric genes that can be functionally expressed in plants and in plant cells (e.g., Klee, 1987, Arm. Rev. of Plant Phys. 38:467-486; Clark et al., 1990, Virology 179(2):640-7; Smith et al., 1990, Mol. Gen. Genet. 224(3):477-81.
The introduction of nucleic acids into plants can be achieved using several technologies including transformation with Agrobacterium tumefaciens or Agrobacterium rhizogenes. Non-limiting examples of plant tissues that can be transformed include protoplasts, microspores or pollen, and explants such as leaves, stems, roots, hypocotyls, and cotyls. Furthermore, DNA encoding a variant CBH I can be introduced directly into protoplasts and plant cells or tissues by microinjection, electroporation, particle bombardment, and direct DNA uptake.
Variant CBH I polypeptides can be produced in plants by a variety of expression systems. For instance, the use of a constitutive promoter such as the 35S promoter of Cauliflower Mosaic Virus (Guilley et al., 1982, Cell 30:763-73) is serviceable for the accumulation of the expressed protein in virtually all organs of the transgenic plant. Alternatively, promoters that are tissue-specific and/or stage-specific can be used (Higgins, 1984, Annu. Rev. Plant Physiol. 35:191-221; Shotwell and Larkins, 1989, In:The Biochemistry of Plants Vol. 15 (Academic Press, San Diego: Stumpf and Conn, eds.), p. 297), permit expression of variant CBH I polypeptides in a target tissue and/or during a desired stage of development.
Compositions Of Variant Cbh I Polypeptides
In general, a variant CBH I polypeptide produced in cell culture is secreted into the medium and may be purified or isolated, e.g., by removing unwanted components from the cell culture medium. However, in some cases, a variant CBH I polypeptide may be produced in a cellular form necessitating recovery from a cell lysate. In such cases the variant CBH I polypeptide is purified from the cells in which it was produced using techniques routinely employed by those of skill in the art. Examples include, but are not limited to, affinity chromatography (Van Tilbeurgh et al., 1984, FEBS Lett. 169(2):215-218), ion-exchange chromatographic methods (Goyal et al., 1991, Bioresource Technology, 36:37-50; Fliess et al., 1983, Eur. J. Appl. Microbiol. Biotechnol. 17:314-318; Bhikhabhai et al., 1984, J. Appl. Biochem. 6:336-345; Ellouz et al., 1987, Journal of Chromatography, 396:307-317), including ion-exchange using materials with high resolution power (Medve et al., 1998, J. Chromatography A, 808:153-165), hydrophobic interaction chromatography (Tomaz and Queiroz, 1999, J. Chromatography A, 865:123-128), and two-phase partitioning (Brumbauer et al., 1999, Bioseparation 7:287-295).
The variant CBH I polypeptides of the disclosure are suitably used in cellulase compositions. Cellulases are known in the art as enzymes that hydrolyze cellulose (beta-1,4-glucan or beta D-glucosidic linkages) resulting in the formation of glucose, cellobiose, cellooligosaccharides, and the like. Cellulase enzymes have been traditionally divided into three major classes: endoglucanases (“EG”), exoglucanases or cellobiohydrolases (EC 3.2.1.91) (“CBH”) and beta-glucosidases (EC 3.2.1.21) (“BG”) (Knowles et al., 1987, TIBTECH 5:255-261; Schulein, 1988, Methods in Enzymology 160(25):234-243).
Certain fungi produce complete cellulase systems which include exo-cellobiohydrolases or CBH-type cellulases, endoglucanases or EG-type cellulases and (3-glucosidases or BG-type cellulases (Schulein, 1988, Methods in Enzymology 160(25):234-243). Such cellulase compositions are referred to herein as “whole” cellulases. However, sometimes these systems lack CBH-type cellulases and bacterial cellulases also typically include little or no CBH-type cellulases. In addition, it has been shown that the EG components and CBH components synergistically interact to more efficiently degrade cellulose. See, e.g., Wood, 1985, Biochemical Society Transactions 13(2):407-410.
The cellulase compositions of the disclosure typically include, in addition to a variant CBH I polypeptide, one or more cellobiohydrolases, endoglucanases and/or β-glucosidases. In their crudest form, cellulase compositions contain the microorganism culture that produced the enzyme components. “Cellulase compositions” also refers to a crude fermentation product of the microorganisms. A crude fermentation is preferably a fermentation broth that has been separated from the microorganism cells and/or cellular debris (e.g., by centrifugation and/or filtration). In some cases, the enzymes in the broth can be optionally diluted, concentrated, partially purified or purified and/or dried. The variant CBH I polypeptide can be co-expressed with one or more of the other components of the cellulase composition or it can be expressed separately, optionally purified and combined with a composition comprising one or more of the other cellulase components.
When employed in cellulase compositions, the variant CBH I is generally present in an amount sufficient to allow release of soluble sugars from the biomass. The amount of variant CBH I enzymes added depends upon the type of biomass to be saccharified which can be readily determined by the skilled artisan. In certain embodiments, the weight percent of variant CBH I polypeptide is suitably at least 1, at least 5, at least 10, or at least 20 weight percent of the total polypeptides in a cellulase composition. Exemplary cellulase compositions include a variant CBH I of the disclosure in an amount ranging from about 1 to about 20 weight percent, from about 1 to about 25 weight percent, from about 5 to about 20 weight percent, from about 5 to about 25 weight percent, from about 5 to about 30 weight percent, from about 5 to about 35 weight percent, from about 5 to about 40 weight percent, from about 5 to about 45 weight percent, from about 5 to about 50 weight percent, from about 10 to about 20 weight percent, from about 10 to about 25 weight percent, from about 10 to about 30 weight percent, from about 10 to about 35 weight percent, from about 10 to about 40 weight percent, from about 10 to about 45 weight percent, from about 10 to about 50 weight percent, from about 15 to about 20 weight percent, from about 15 to about 25 weight percent, from about 15 to about 30 weight percent, from about 15 to about 35 weight percent, from about 15 to about 30 weight percent, from about 15 to about 45 weight percent, or from about 15 to about 50 weight percent of the total polypeptides in the composition.
Utility of Variant CBH I Polypeptides
It can be appreciated that the variant CBH I polypeptides of the disclosure and compositions comprising the variant CBH I polypeptides find utility in a wide variety applications, for example detergent compositions that exhibit enhanced cleaning ability, function as a softening agent and/or improve the feel of cotton fabrics (e.g., “stone washing” or “biopolishing”), or in cellulase compositions for degrading wood pulp into sugars (e.g., for bio-ethanol production). Other applications include the treatment of mechanical pulp (Pere et al., 1996, Tappi Pulping Conference, pp. 693-696 (Nashville, Tenn., Oct. 27-31, 1996)), for use as a feed additive (see, e.g., WO 91/04673) and in grain wet milling.
Saccharification Reactions
Ethanol can be produced via saccharification and fermentation processes from cellulosic biomass such as trees, herbaceous plants, municipal solid waste and agricultural and forestry residues. However, the ratio of individual cellulase enzymes within a naturally occurring cellulase mixture produced by a microbe may not be the most efficient for rapid conversion of cellulose in biomass to glucose. It is known that endoglucanases act to produce new cellulose chain ends which themselves are substrates for the action of cellobiohydrolases and thereby improve the efficiency of hydrolysis of the entire cellulase system. The use of optimized cellobiohydrolase activity may greatly enhance the production of ethanol.
Cellulase compositions comprising one or more of the variant CBH I polypeptides of the disclosure can be used in saccharification reaction to produce simple sugars for fermentation. Accordingly, the present disclosure provides methods for saccharification comprising contacting biomass with a cellulase composition comprising a variant CBH I polypeptide of the disclosure and, optionally, subjecting the resulting sugars to fermentation by a microorganism.
The term “biomass,” as used herein, refers to any composition comprising cellulose (optionally also hemicellulose and/or lignin). As used herein, biomass includes, without limitation, seeds, grains, tubers, plant waste or byproducts of food processing or industrial processing (e.g., stalks), corn (including, e.g., cobs, stover, and the like), grasses (including, e.g., Indian grass, such as Sorghastrum nutans; or, switchgrass, e.g., Panicum species, such as Panicum virgatum), wood (including, e.g., wood chips, processing waste), paper, pulp, and recycled paper (including, e.g., newspaper, printer paper, and the like). Other biomass materials include, without limitation, potatoes, soybean (e.g., rapeseed), barley, rye, oats, wheat, beets, and sugar cane bagasse.
The saccharified biomass (e.g., lignocellulosic material processed by enzymes of the disclosure) can be made into a number of bio-based products, via processes such as, e.g., microbial fermentation and/or chemical synthesis. As used herein, “microbial fermentation” refers to a process of growing and harvesting fermenting microorganisms under suitable conditions. The fermenting microorganism can be any microorganism suitable for use in a desired fermentation process for the production of bio-based products. Suitable fermenting microorganisms include, without limitation, filamentous fungi, yeast, and bacteria. The saccharified biomass can, for example, be made it into a fuel (e.g., a biofuel such as a bioethanol, biobutanol, biomethanol, a biopropanol, a biodiesel, a jet fuel, or the like) via fermentation and/or chemical synthesis. The saccharified biomass can, for example, also be made into a commodity chemical (e.g., ascorbic acid, isoprene, 1,3-propanediol), lipids, amino acids, polypeptides, and enzymes, via fermentation and/or chemical synthesis.
Thus, in certain aspects, the variant CBH I polypeptides of the disclosure find utility in the generation of ethanol from biomass in either separate or simultaneous saccharification and fermentation processes. Separate saccharification and fermentation is a process whereby cellulose present in biomass is saccharified into simple sugars (e.g., glucose) and the simple sugars subsequently fermented by microorganisms (e.g., yeast) into ethanol. Simultaneous saccharification and fermentation is a process whereby cellulose present in biomass is saccharified into simple sugars (e.g., glucose) and, at the same time and in the same reactor, microorganisms (e.g., yeast) ferment the simple sugars into ethanol.
Prior to saccharification, biomass is preferably subject to one or more pretreatment step(s) in order to render cellulose material more accessible or susceptible to enzymes and thus more amenable to hydrolysis by the variant CBH I polypeptides of the disclosure.
In an exemplary embodiment, the pretreatment entails subjecting biomass material to a catalyst comprising a dilute solution of a strong acid and a metal salt in a reactor. The biomass material can, e.g., be a raw material or a dried material. This pretreatment can lower the activation energy, or the temperature, of cellulose hydrolysis, ultimately allowing higher yields of fermentable sugars. See, e.g., U.S. Pat. Nos. 6,660,506; 6,423,145.
Another exemplary pretreatment method entails hydrolyzing biomass by subjecting the biomass material to a first hydrolysis step in an aqueous medium at a temperature and a pressure chosen to effectuate primarily depolymerization of hemicellulose without achieving significant depolymerization of cellulose into glucose. This step yields a slurry in which the liquid aqueous phase contains dissolved monosaccharides resulting from depolymerization of hemicellulose, and a solid phase containing cellulose and lignin. The slurry is then subject to a second hydrolysis step under conditions that allow a major portion of the cellulose to be depolymerized, yielding a liquid aqueous phase containing dissolved/soluble depolymerization products of cellulose. See, e.g., U.S. Pat. No. 5,536,325.
A further exemplary method involves processing a biomass material by one or more stages of dilute acid hydrolysis using about 0.4% to about 2% of a strong acid; followed by treating the unreacted solid lignocellulosic component of the acid hydrolyzed material with alkaline delignification. See, e.g., U.S. Pat. No. 6,409,841. Another exemplary pretreatment method comprises prehydrolyzing biomass (e.g., lignocellulosic materials) in a prehydrolysis reactor; adding an acidic liquid to the solid lignocellulosic material to make a mixture; heating the mixture to reaction temperature; maintaining reaction temperature for a period of time sufficient to fractionate the lignocellulosic material into a solubilized portion containing at least about 20% of the lignin from the lignocellulosic material, and a solid fraction containing cellulose; separating the solubilized portion from the solid fraction, and removing the solubilized portion while at or near reaction temperature; and recovering the solubilized portion. The cellulose in the solid fraction is rendered more amenable to enzymatic digestion. See, e.g., U.S. Pat. No. 5,705,369. Further pretreatment methods can involve the use of hydrogen peroxide H2O2. See Gould, 1984, Biotech, and Bioengr. 26:46-52.
Pretreatment can also comprise contacting a biomass material with stoichiometric amounts of sodium hydroxide and ammonium hydroxide at a very low concentration. See Teixeira et al., 1999, Appl. Biochem. and Biotech. 77-79:19-34. Pretreatment can also comprise contacting a lignocellulose with a chemical (e.g., a base, such as sodium carbonate or potassium hydroxide) at a pH of about 9 to about 14 at moderate temperature, pressure, and pH. See PCT Publication WO2004/081185.
Ammonia pretreatment can also be used. Such a pretreatment method comprises subjecting a biomass material to low ammonia concentration under conditions of high solids. See, e.g., U.S. Patent Publication No. 20070031918 and PCT publication WO 06/110901.
Detergent Compositions Comprising Variant CBH I Proteins
The present disclosure also provides detergent compositions comprising a variant CBH I polypeptide of the disclosure. The detergent compositions may employ besides the variant CBH I polypeptide one or more of a surfactant, including anionic, non-ionic and ampholytic surfactants; a hydrolase; a bleaching agents; a bluing agent; a caking inhibitors; a solubilizer; and a cationic surfactant. All of these components are known in the detergent art.
The variant CBH I polypeptide is preferably provided as part of cellulase composition. The cellulase composition can be employed from about 0.00005 weight percent to about 5 weight percent or from about 0.0002 weight percent to about 2 weight percent of the total detergent composition. The cellulase composition can be in the form of a liquid diluent, granule, emulsion, gel, paste, and the like. Such forms are known to the skilled artisan. When a solid detergent composition is employed, the cellulase composition is preferably formulated as granules.
EXAMPLES
Materials and Methods
Preparation Of CBH I Polypeptides For Biochemical Characterization
Protein expression was carried out in an Aspergillus niger host strain that had been transformed using PEG-mediated transformation with expression constructs for CBHI that included the hygromycin resistance gene as a selectable marker, in which the full length CBH I sequences (signal sequence, catalytic domain, linker and cellulose binding domain) were under the control of the glyceraldeyhde-3-phosphate dehydrogenase (gpd) promoter. Transformants were selected on the regeneration medium based on resistance to hygromycin. The selected transformants were cultured in Aspergillus salts medium, pH 6.2 supplemented with the antibiotics penicillin, streptomycin, and hygromycin, and 80 g/L glycerol, 20 g/L soytone, 10 mM uridine, 20 g/L MES) in baffled shake flasks at 30° C., 170 rpm. After five days of incubation, the total secreted protein supernatant was recovered, and then subjected to hollow fiber filtration to concentrate and exchange the sample into acetate buffer (50 mM NaAc, pH 5). CBH I protein represented over 90% of the total protein in these samples. Protein purity was analyzed by SDS-PAGE. Protein concentration was determined by gel densitometry and/or HPLC analysis. All CBH I protein concentrations were normalized before assay and concentrated to 1-2.5 mg/ml.
CBH I Activity Assays
4-Methylumbelliferyl Lactoside (4-MUL) Assay: This assay measures the activity of CBH I on the fluorogenic substrate 4-MUL (also known as MUL). Assays were run in a costar 96-well black bottom plate, where reactions were initiated by the addition of 4-MUL to enzyme in buffer (2 mM 4-MUL in 200 mM MES pH 6). Enzymatic rates were monitored by fluorescent readouts over five minutes on a SPECTRAMAXTM plate reader (ex/em 365/450 nm). Data in the linear range was used to calculate initial rates (Vo).
Phosphoric Acid Swollen Cellulose (PASC) Assay: This assay measures the activity of CBH I using PASC as the substrate. During the assay, the concentration of PASC is monitored by a fluorescent signal derived from calcofluor binding to PASC (ex/em 365/440 nm). The assay is initiated by mixing enzyme (15 μl) and reaction buffer (85 μl of 0.2% PASC, 200 mM MES, pH 6), and then incubating at 35° C. while shaking at 225 RPM. After 2 hours, one reaction volume of calcofluor stop solution (100 μg/ml in 500 mM glycine pH 10) is added and fluorescence read-outs obtained (ex/em 365/440 nm).
Bagasse Assay: This assay measures the activity of CBH I on bagasse, a lignocellulosic substrate. Reactions were run in 10 ml vials with 5% dilute acid pretreated bagasse (250 mg solids per 5 ml reaction). Each reaction contained 4 mg CBH I enzyme/g solids, 200 mM MES pH 6, kanamycin, and chloramphenicol. Reactions were incubated at 35° C. in hybridization incubators (Robbins Scientific), rotating at 20 RPM. Time points were taken by transferring a sample of homogenous slurry (150 μl) into a 96-well deep well plate and quenching the reaction with stop buffer (450 μl of 500 mM sodium carbonate, pH 10). Time point measurements were taken every 24 hours for 72 hours.
Cellobiose Tolerance Assays (or Cellobiose Inhibition Assays): Tolerance to cellobiose (or inhibition caused by cellobiose) was tested in two ways in the CBH I assays. A direct-dose tolerance method can be applied to all of the CBH I assays (i.e., 4-MUL, PASC, and/or bagasse assays), and entails the exogenous addition of a known amount of cellobiose into assay mixtures. A different indirect method entails the addition of an excess amount of β-glucosidase (BG) to PASC and bagasse assays (typically, 1 mg β-glucosidase/g solids loaded). BG will enzymatically hydrolyze the cellobiose generated during these assays; therefore, CBH I activity in the presence of BG can be taken as a measure of activity in the absence of cellobiose. Furthermore, when activity in the presence and absence of BG are similar, this indicates tolerance to cellobiose. Notably, in cases where BG activity is undesired, but may be present in crude CBH I enzyme preparations, the BG inhibitor gluconolactone can be added into CBH I assays to prevent cellobiose breakdown.
Library Screening Assays
The wild type CBH I polypeptide BD29555 was mutagenized to identify variants with improved product tolerance. A small (60-member) library of BD29555 variants was designed to identify variant CBH I polypeptides with reduced product inhibition. This product-release-site library was designed based on residues directly interacting with the cellobiose product in an attempt to identify variants with weakened interactions with cellobiose from which the product would be released more readily than the wild type enzyme. The 60-member evolution library contained wild-type residues and mutations at positions R273, W405, and R422 of BD29555 (SEQ ID NO:1), and included the following substitutions: R273 (WT), R273Q, R273K, R273A, W405 (WT), W405Q, W405H, R422 (WT), R422Q, R422K, R422L, and R422E (4 variants at position 273×3 variants at position 405×5 variants at position 422 equals 60 variants in total). All members of the library were screened using the 4-MUL assay in the presence and absence of 250 g/L cellobiose and using gluconolactone to inhibit any BG activity. The R273A, R273Q, and R273K/R422K variants showed enhanced product tolerance. The R273K/R422K variant showed greatest activity among the variants and cellobiose tolerance at 250 mg/L. Due to low expression, the R273K variant was not tested for product inhibition.
Characterization of Product Tolerant Variants of BD29555
The R273K/R422K substitutions were characterized in both a wild type BD29555 background and also in combination with the substitutions Y274Q, D281K, Y410H, P411G, which were identified in a screen of an expanded product release site evolution library.
The wild type, the R273K/R422K variant and the R273K/Y274Q/D281K/Y410H/P411G/R422K variants were tested for activity on 4-MUL in the presence and absence of 250 mg/L cellobiose, and the R273K/R422K variant was also tested in the bagasse assay in the presence and absence of BG. The results are summarized in Table 5.
The results from these activity assays were converted into the percentage of activity remaining with and without cellobiose present, where values close to 100% indicated cellobiose tolerance. The percent of activity remaining in the MUL assay in the presence cellobiose versus in the absence of cellobiose shows that the R273K/R422K variant was the most tolerant, followed by the R273K/Y274Q/D281K/Y410H/P411G/R422K variant, and then wild-type, at 95%, 78%, and 25% activity, respectively.
Cellobiose dose response curves of the wild-type and R273K/R422K variant of BD29555 were obtained during the 4-MUL assay. Enzyme rates (Vo) were measured in the presence of different concentrations of cellobiose (200 mM MES pH 6, 25° C.). Rates were measured in quadruplicate. The results are shown in FIG. 1A-1B. FIG. 1A shows that wild type BD2955 is inhibited by cellobiose, with a half maximal inhibitory concentration (IC50 value) of 60 mg/L. FIG. 1B shows that the R273K/R422K variant is tolerant to cellobiose up to 250 mg/L.
The bagasse assay results shown in Table 5, which lists the percentage of activity remaining in the absence vs. presence of BG, also demonstrate that the percentage activity of the wild type BD29555 is lower than the percentage activity of the R273K/R422K variant, indicating that the R273K/R422K variant is less sensitive to the presence of cellobiose than the wild type. FIG. 2A-2B shows bar graph data for the bagasse assay of BD29555 vs. the R273K/R422K variant. In FIG. 2A, bars represent relative activity, which has been normalized to wild type activity in the absence of cellobiose (WT+BG=uninhibited activity=1). In FIG. 2B, bars indicate tolerance to cellobiose, as represented by the ratio of activity in the presence of cellobiose (−BG) to that of activity in the absence of cellobiose (+BG); ratios close to 1 indicate greater tolerance to cellobiose. These data again demonstrate that the R273K/R422K variant of BD29555 is more tolerant to cellobiose than the wild type BD29555.
The wild type and R273K/R422K variant were also characterized in the PASC assay. Results are shown in FIG. 3. The activities of both wild type BD29555 (SEQ ID NO:1) and wild type T. reesei CBH I (SEQ ID NO:2) were inhibited by cellobiose concentrations starting around 1 g/L (with IC50 values of 2.2 and 3 g/L, respectively), whereas the R273K/R422K variant showed little inhibition in the presence of 10 g/L cellobiose.
Characterization of Product Tolerant Variants of T. Reesei CBH I
Cellobiose product tolerant substitutions were introduced into T. reesei CBH I (SEQ ID NO:2). A panel of variants with single and double alanine and lysine substitutions at R268 and R411 were expressed and analyzed. The variants were tested for activity on 4-MUL in the presence and absence of 250 mg/L cellobiose and also in the bagasse assay in the absence and presence of BG. The results from these assays were converted into the percentage activity remaining in the presence and absence of cellobiose and BG, respectively. Values are summarized in Table 6.
The 4-MUL assay results shown in Table 6 demonstrate that the activity of the wild type T. reesei CBH I was reduced to 23% in the presence of cellobiose, whereas the double mutants at R268 and R411 retained more than 90% of their activity under the same conditions.
The bagasse assay results shown in Table 6 demonstrate that the activity of the wild type T. reesei CBH I is more significantly impacted by the presence of BG than is the activity of the single or double substitution variants, indicating that the variants are less sensitive to the accumulation of cellobiose than the wild type. FIGS. 4 and 5 show bar graph data for the bagasse assay of wild type T. reesei CBH I vs. the variants. In FIG. 4, bars represent relative activity, normalized to wild type activity in the absence of cellobiose (WT+BG=1). In FIG. 5, bars represent tolerance to cellobiose, as represented by the ratio of activity in the presence of accumulating cellobiose (−BG) to that of activity in the absence of cellobiose (+BG); ratios close to 1 indicate greater tolerance to cellobiose.
Specific Embodiments and Incorporation by Reference
All publications, patents, patent applications and other documents cited in this application are hereby incorporated by reference in their entireties for all purposes to the same extent as if each individual publication, patent, patent application or other document were individually indicated to be incorporated by reference for all purposes.
While various specific embodiments have been illustrated and described, it will be appreciated, that various changes can be made without departing from the spirit and scope of the invention(s).










TABLE 1





Sequence Identifier
Database




(SEQ ID NO:)
Accession Number
Species of Origin
Amino acid sequence







SEQ ID NO: 1
BD29555*
Unknown
MSALNSFNMY KSALILGSLL ATAGAQQIGT YTAETHPSLS WSTCKSGGSC TTNSGAITLD ANWRWVHGVN





TSTNCYTGNT WNTAICDTDA SCAQDCALDG ADYSGTYGIT TSGNSLRLNF VTGSNVGSRT YLMADNTHYQ





IFDLLNQEFT FTVDVSHLPC GLNGALYFVT MDADGGVSKY PNNKAGAQYG VGYCDSQCPR DLKFIAGQAN





VEGWTPSSNN ANTGLGNHGA CCAELDIWEA NSISEALTPH PCDTPGLSVC TTDACGGTYS SDRYAGTCDP





DGCDFNPYRL GVTDFYGSGK TVDTTKPITV VTQFVTDDGT STGTLSEIRR YYVQNGVVIP QPSSKISGVS





GNVINSDFCD AEISTFGETA SFSKHGGLAK MGAGMEAGMV LVMSLWDDYS VNMLWLDSTY PTNATGTPGA





ARGSCPTTSG DPKTVESQSG SSYVTFSDIR VGPFNSTFSG GSSTGGSSTT TASGTTTTKA SSTSTSSTST





GTGVAAHWGQ CGGQGWTGPT TCASGTTCTV VNPYYSQCL


 


SEQ ID NO: 2
340514556
Trichoderma reesei
MYRKLAVISA FLATARAQSA CTLQSETHPP LTWQKCSSGG TCTQQTGSVV IDANWRWTHA TNSSTNCYDG





NTWSSTLCPD NETCAKNCCL DGAAYASTYG VTTSGNSLSI GFVTQSAQKN VGARLYLMAS DTTYQEFTLL





GNEFSFDVDV SQLPCGLNGA LYFVSMDADG GVSKYPTNTA GAKYGTGYCD SQCPRDLKFI NGQANVEGWE





PSSNNANTGI GGHGSCCSEM DIWEANSISE ALTPHPCTTV GQEICEGDGC GGTYSDNRYG GTCDPDGCDW





NPYRLGNTSF YGPGSSFTLD TTKKLTVVTQ FETSGAINRY YVQNGVTFQQ PNAELGSYSG NELNDDYCTA





EEAEFGGSSF SDKGGLTQFK KATSGGMVLV MSLWDDYYAN MLWLDSTYPT NETSSTPGAV RGSCSTSSGV





PAQVESQSPN AKVTFSNIKF GPIGSTGNPS GGNPPGGNPP GTTTTRRPAT TTGSSPGPTQ SHYGQCGGIG





YSGPTVCASG TTCQVLNPYY SQCL


 


SEQ ID NO: 3
51243029
Penicillium occitanis
MSALNSFNMY KSALILGSLL ATAGAQQIGT YTAETHPSLS WSTCKSGGSC TTNSGAITLD ANWRWVHGVN





TSTNCYTGNT WNSAICDTDA SCAQDCALDG ADYSGTYGIT TSGNSLRLNF VTGSNVGSRT YLMADNTHYQ





IFDLLNQEFT FTVDVSHLPC GLNGALYFVT MDADGGVSKY PNNKAGAQYG VGYCDSQCPR DLKFIAGQAN





VEGWTPSANN ANTGIGNHGA CCAELDIWEA NSISEALTPH PCDTPGLSVC TTDACGGTYS SDRYAGTCDP





DGCDFNPYRL GVTDFYGSGK TVDTTKPFTV VTQFVTNDGT STGSLSEIRR YYVQNGVVIP QPSSKISGIS





GNVINSDYCA AEISTFGGTA SFNKHGGLTN MAAGMEAGMV LVMSLWDDYA VNMLWLDSTY PTNATGTPGA





ARGTCATTSG DPKTVESQSG SSYVTFSDIR VGPFNSTFSG GSSTGGSTTT TASRTTTTSA SSTSTSSTST





GTGVAGHWGQ CGGQGWTGPT TCVSGTTCTV VNPYYSQCL


 


SEQ ID NO: 4
7cel (PDB) &
Trichoderma reesei
ESACTLQSET HPPLTWQKCS SGGTCTQQTG SVVIDANWRW THATNSSTNC YDGNTWSSTL CPDNETCAKN





CCLDGAAYAS TYGVTTSGNS LSIDFVTQSA QKNVGARLYL MASDTTYQEF TLLGNEFSFD VDVSQLPCGL





NGALYFVSMD ADGGVSKYPT NTAGAKYGTG YCDSQCPRDL KFINGQANVE GWEPSSNNAN TGIGGHGSCC





SEMDIWQANS ISEALTPHPC TTVGQEICEG DGCGGTYSDN RYGGTCDPDG CDWNPYRLGN TSFYGPGSSF





TLDTTKKLTV VTQFETSGAI NRYYVQNGVT FQQPNAELGS YSGNELNDDY CTAEEAEFGG SSFSDKGGLT





QFKKATSGGM VLVMSLWDDY YANMLWLDST YPTNETSSTP GAVRGSCSTS SGVPAQVESQ SPNAKVTFSN





IKFGPIGSTG NPSG


 


SEQ ID NO: 5
67516425
Aspergillus nidulans
MASSFQLYKA LLFFSSLLSA VQAQKVGTQQ AEVHPGLTWQ TCTSSGSCTT VNGEVTIDAN WRWLHTVNGY




FGSC A4
TNCYTGNEWD TSICTSNEVC AEQCAVDGAN YASTYGITTS GSSLRLNFVT QSQQKNIGSR VYLMDDEDTY





TMFYLLNKEF TFDVDVSELP CGLNGAVYFV SMDADGGKSR YATNEAGAKY GTGYCDSQCP RDLKFINGVA





NVEGWESSDT NPNGGVGNHG SCCAEMDIWE ANSISTAFTP HPCDTPGQTL CTGDSCGGTY SNDRYGGTCD





PDGCDFNSYR QGNKTFYGPG LTVDTNSPVT VVTQFLTDDN TDTGTLSEIK RFYVQNGVVI PNSESTYPAN





PGNSITTEFC ESQKELFGDV DVFSAHGGMA GMGAALEQGM VLVLSLWDDN YSNMLWLDSN YPTDADPTQP





GIARGTCPTD SGVPSEVEAQ YPNAYVVYSN IKFGPIGSTF GNGGGSGPTT TVTTSTATST TSSATSTATG





QAQHWEQCGG NGWTGPTVCA SPWACTVVNS WYSQCL


 


SEQ ID NO: 6
46107376
Gibberella zeae PH-1
MYRAIATASA LIAAVRAQQV CSLTQESKPS LNWSKCTSSG CSNVKGSVTI DANWRWTHQV SGSTNCYTGN





KWDTSVCTSG KVCAEKCCLD GADYASTYGI TSSGDQLSLS FVTKGPYSTN IGSRTYLMED ENTYQMFQLL





GNEFTFDVDV SNIGCGLNGA LYFVSMDADG GKAKYPGNKA GAKYGTGYCD AQCPRDVKFI NGQANSDGWQ





PSDSDVNGGI GNLGTCCPEM DIWEANSIST AYTPHPCTKL TQHSCTGDSC GGTYSNDRYG GTCDADGCDF





NSYRQGNKTF YGPGSGFNVD TTKKVTVVTQ FHKGSNGRLS EITRLYVQNG KVIANSESKI AGVPGNSLTA





DFCTKQKKVF NDPDDFTKKG AWSGMSDALE APMVLVMSLW HDHHSNMLWL DSTYPTDSTK LGSQRGSCST





SSGVPADLEK NVPNSKVAFS NIKFGPIGST YKSDGTTPTN PTNPSEPSNT ANPNPGTVDQ WGQCGGSNYS





GPTACKSGFT CKKINDFYSQ CQ


 


SEQ ID NO: 7
70992391
Aspergillus
MLASTFSYRM YKTALILAAL LGSGQAQQVG TSQAEVHPSM TWQSCTAGGS CTTNNGKVVI DANWRWVHKV




fumigatus Af293
GDYTNCYTGN TWDTTICPDD ATCASNCALE GANYESTYGV TASGNSLRLN FVTTSQQKNI GSRLYMMKDD





STYEMFKLLN QEFTFDVDVS NLPCGLNGAL YFVAMDADGG MSKYPTNKAG AKYGTGYCDS QCPRDLKFIN





GQANVEGWQP SSNDANAGTG NHGSCCAEMD IWEANSISTA FTPHPCDTPG QVMCTGDACG GTYSSDRYGG





TCDPDGCDFN SFRQGNKTFY GPGMTVDTKS KFTVVTQFIT DDGTSSGTLK EIKRFYVQNG KVIPNSESTW





TGVSGNSITT EYCTAQKSLF QDQNVFEKHG GLEGMGAALA QGMVLVMSLW DDHSANMLWL DSNYPTTASS





TTPGVARGTC DISSGVPADV EANHPDAYVV YSNIKVGPIG STFNSGGSNP GGGTTTTTTT QPTTTTTTAG





NPGGTGVAQH YGQCGGIGWT GPTTCASPYT CQKLNDYYSQ CL


 


SEQ ID NO: 8
121699984
Aspergillus clavatus
MLPSTISYRI YKNALFFAAL FGAVQAQKVG TSKAEVHPSM AWQTCAADGT CTTKNGKVVI DANWRWVHDV




NRRL 1
KGYTNCYTGN TWNAELCPDN ESCAENCALE GADYAATYGA TTSGNALSLK FVTQSQQKNI GSRLYMMKDD





NTYETFKLLN QEFTFDVDVS NLPCGLNGAL YFVSMDADGG LSRYTGNEAG AKYGTGYCDS QCPRDLKFIN





GLANVEGWTP SSSDANAGNG GHGSCCAEMD IWEANSISTA YTPHPCDTPG QAMCNGDSCG GTYSSDRYGG





TCDPDGCDFN SYRQGNKSFY GPGMTVDTKK KMTVVTQFLT NDGTATGTLS EIKRFYVQDG KVIANSESTW





PNLGGNSLTN DFCKAQKTVF GDMDTFSKHG GMEGMGAALA EGMVLVMSLW DDHNSNMLWL DSNSPTTGTS





TTPGVARGSC DISSGDPKDL EANHPDASVV YSNIKVGPIG STFNSGGSNP GGSTTTTKPA TSTTTTKATT





TATTNTTGPT GTGVAQPWAQ CGGIGYSGPT QCAAPYTCTK QNDYYSQCL


 


SEQ ID NO: 9
1906845
Claviceps purpurea
MHPSLQTILL SALFTTAHAQ QACSSKPETH PPLSWSRCSR SGCRSVQGAV TVDANWLWTT VDGSQNCYTG





NRWDTSICSS EKTCSESCCI DGADYAGTYG VTTTGDALSL KFVQQGPYSK NVGSRLYLMK DESRYEMFTL





LGNEFTFDVD VSKLGCGLNG ALYFVSMDED GGMKRFPMNK AGAKFGTGYC DSQCPRDVKF INGMANSKDW





IPSKSDANAG IGSLGACCRE MDIWEANNIA SAFTPHPCKN SAYHSCTGDG CGGTYSKNRY SGDCDPDGCD





FNSYRLGNTT FYGPGPKFTI DTTRKISVVT QFLKGRDGSL REIKRFYVQN GKVIPNSVSR VRGVPGNSIT





QGFCNAQKKM FGAHESFNAK GGMKGMSAAV SKPMVLVMSL WDDHNSNMLW LDSTYPTNSR QRGSKRGSCP





ASSGRPTDVE SSAPDSTVVF SNIKFGPIGS TFSRGK


 


SEQ ID NO: 10
1gpi (PDB) &
Phanerochaete
ESACTLQSET HPPLTWQKCS SGGTCTQQTG SVVIDANWRW THATNSSTNC YDGNTWSSTL CPDNETCAKN




chrysosporium
CCLDGAAYAS TYGVTTSGNS LSIDFVTQSA QKNVGARLYL MASDTTYQEF TLLGNEFSFD VDVSQLPCGL





NGALYFVSMD ADGGVSKYPT NTAGAKYGTG YCDSQCPRDL KFINGQANVE GWEPSSNNAN TGIGGHGSCC





SEMDIWQANS ISEALTPHPC TTVGQEICEG DGCGGTYSDN RYGGTCDPDG CDWNPYRLGN TSFYGPGSSF





TLDTTKKLTV VTQFETSGAI NRYYVQNGVT FQQPNAELGS YSGNELNDDY CTAEEAEFGG SSFSDKGGLT





QFKKATSGGM VLVMSLWDDY YANMLWLDST YPTNETSSTP GAVRGSCSTS SGVPAQVESQ SPNAKVTFSN





IKFGPIGSTG NPSG


 


SEQ ID NO: 11
119468034
Neosartorya fischeri
MHQRALLFSA LAVAANAQQV GTQKPETHPP LTWQKCTAAG SCSQQSGSVV IDANWRWLHS TKDTTNCYTG




NRRL 181
NTWNTELCPD NESCAQNCAV DGADYAGTYG VTTSGSELKL SFVTGANVGS RLYLMQDDET YQHFNLLNNE





FTFDVDVSNL PCGLNGALYF VAMDADGGMS KYPSNKAGAK YGTGYCDSQC PRDLKFINGM ANVEGWKPSS





NDKNAGVGGH GSCCPEMDIW EANSISTAVT PHPCDDVSQT MCSGDACGGT YSATRYAGTC DPDGCDFNPF





RMGNESFYGP GKIVDTKSEM TVVTQFITAD GTDTGALSEI KRLYVQNGKV IANSVSNVAD VSGNSISSDF





CTAQKKAFGD EDIFAKHGGL SGMGKALSEM VLIMSIWDDH HSSMMWLDST YPTDADPSKP GVARGTCEHG





AGDPEKVESQ HPDASVTFSN IKFGPIGSTY KA


 


SEQ ID NO: 12
7804883
Leptosphaeria
MYRSLIFATS LLSLAKGQLV GNLYCKGSCT AKNGKVVIDA NWRWLHVKGG YTNCYTGNEW NATACPDNKS




maculans
CATNCAIDGA DYRRLRHYCE RQLLGTEVHH QGLYSTNIGS RTYLMQDDST YQLFKFTGSQ EFTFDVDLSN





LPCGLNGALY FVSMDADGGL KKYPTNKAGA KYGTGYCDAQ CPRDLKFING EGNVEGWQPS KNDQNAGVGG





HGSCCAEMDI WEANSVSTAV TPHSCSTIEQ SRCDGDGCGG TYSADRYAGV CDPDGCDFNS YRMGVKDFYG





KGKTVDTSKK FTVVTQFIGS GDAMEIKRFY VQNGKTIPQP DSTIPGVTGN SITTFFCDAQ KKAFGDKYTF





KDKGGMANMP STCNGMVLVM SLWDDHYSNM LWLDSTYPTD KNPDTDAGSG RGECAITSGV PADVESQHPD





ASVIYSNIKF GPINTTFG


 


SEQ ID NO: 13
85108032
Neurospora crassa
MLAKFAALAA LVASANAQAV CSLTAETHPS LNWSKCTSSG CTNVAGSITV DANWRWTHIT SGSTNCYSGN




N150(OR74A)
EWDTSLCSTN TDCATKCCVD GAEYSSTYGI QTSGNSLSLQ FVTKGSYSTN IGSRTYLMNG ADAYQGFELL





GNEFTFDVDV SGTGCGLNGA LYFVSMDLDG GKAKYTNNKA GAKYGTGYCD AQCPRDLKYI NGIANVEGWT





PSTNDANAGI GDHGTCCSEM DIWEANKVST AFTPHPCTTI EQHMCEGDSC GGTYSDDRYG GTCDADGCDF





NSYRMGNTTF YGEGKTVDTS SKFTVVTQFI KDSAGDLAEI KRFYVQNGKV IENSQSNVDG VSGNSITQSF





CNAQKTAFGD IDDFNKKGGL KQMGKALAKP MVLVMSIWDD HAANMLWLDS TYPVEGGPGA YRGECPTTSG





VPAEVEANAP NSKVIFSNIK FGPIGSTFSG GSSGTPPSNP SSSVKPVTST AKPSSTSTAS NPSGTGAAHW





AQCGGIGFSG PTTCQSPYTC QKINDYYSQC V


 


SEQ ID NO: 14
169859458
Coprinopsis cinerea
MFKKVALTAL CFLAVAQAQQ VGREVAENHP RLPWQRCTRN GGCQTVSNGQ VVLDANWRWL HVTDGYTNCY




okayama
TGNSWNSTVC SDPTTCAQRC ALEGANYQQT YGITTNGDAL TIKFLTRSQQ TNVGARVYLM ENENRYQMFN





LLNKEFTFDV DVSKVPCGIN GALYFIQMDA DGGMSKQPNN RAGAKYGTGY CDSQCPRDIK FIDGVANSAD





WTPSETDPNA GRGRYGICCA EMDIWEANSI SNAYTPHPCR TQNDGGYQRC EGRDCNQPRY EGLCDPDGCD





YNPFRMGNKD FYGPGKTVDT NRKMTVVTQF ITHDNTDTGT LVDIRRLYVQ DGRVIANPPT NFPGLMPAHD





SITEQFCTDQ KNLFGDYSSF ARDGGLAHMG RSLAKGHVLA LSIWNDHGAH MLWLDSNYPT DADPNKPGIA





RGTCPTTGGT PRETEQNHPD AQVIFSNIKF GDIGSTFSGY


 


SEQ ID NO: 15
154292161
Botryotinia
MYSAAVLATF SFLLGAGAQQ VGTSTAETHP ALTVQKCAAG GTCTDESDSI VLDANWRWLH STSGSTNCYT




fuckeliana B05-10
GNTWDTTLCP DAATCTTNCA LDGADYEGTY GITTSGDSLK LSFVTGSNVG SRTYLMDSET TYKEFALLGN





EFTFTVDVSK LPCGLNGALY FVPMDADGGM SKYPTNKAGA KYGTGYCDAQ CPQDMKFVNG TANVEGWVPD





SNSANSGTGN IGSCCSEFDV WEANSMSQAL TPHVCTVDSQ TACTGDDCAS NTGVCDGDGC DFNPYRMGNT





TFYGSGMTID TSKPFSVVTQ FITDDGTETG TLTEIKRFYV QDDVVYEQPS SDISGVSGNS ITDDFCAAQK





TAFGDTDYFT QNGGMAAMGK KMADGMVLVL SIWDDYNVNM LWLDSDYPTT KDASTPGVSR GSCATDSGVP





ATVEAASGSA YVTFSSIKYG PIGSTFNAPA DSSSSVSASS SPAPIASSSS SASIAPVSSV VAAIVSSSAQ





AISSAAPVVS SSAQAISSAA PVVSSVVSSA APVATSSTKS KCSKVSSTLK TSVAAPATSA TSAAVVATSS





AASSTGSVPL YGNCTGGKTC SEGTCVVQND YYSQCVASS


 


SEQ ID NO: 16
169615761 #
haeosphaeria
MTWQRCTGTG GSSCTNVNGE IVIDANWRWI HATGGYTNCF DGNEWNKTAC PSNAACTKNC AIEGSDYRGT




nodorum SN15
YGITTSGNSL TLKFITKGQY STNVGSRTYL MKDTNNYEMF NLIGNEFTFD VDLSQLPCGL NGALYFVSMP





EKGQGTPGAK YGTGKLSQCS VHISKTLTDA CARDLKFVGG EANADGWQAS TSDPNAGVGK KGACCAEMDV





WEANSMSTAL TPHSCQPEGY AVCEESNCGG TYSLDRYAGT CDANGCDFNP YRVGNKDFYG KGKTVDTSKK





MTVVTQFLGT GSDLTELKRF YVQDGKVISN PEPTIPGMTG NSITQKWCDT QKEVFKEEVY PFNQWGGMAS





MGKGMAQGMV LVMSLWDDHY SNMLWLDSTY PTDRDPESPG AARGECAITS GAPAEVEANN PDASVMFSNI





KFGPIGSTFQ QPA


 


SEQ ID NO: 17
4883502
Humicola grisea
MQIKSYIQYL AAALPLLSSV AAQQAGTITA ENHPRMTWKR CSGPGNCQTV QGEVVIDANW RWLHNNGQNC





YEGNKWTSQC SSATDCAQRC ALDGANYQST YGASTSGDSL TLKFVTKHEY GTNIGSRFYL MANQNKYQMF





TLMNNEFAFD VDLSKVECGI NSALYFVAME EDGGMASYPS NRAGAKYGTG YCDAQCARDL KFIGGKANIE





GWRPSTNDPN AGVGPMGACC AEIDVWESNA YAYAFTPHAC GSKNRYHICE TNNCGGTYSD DRFAGYCDAN





GCDYNPYRMG NKDFYGKGKT VDTNRKFTVV SRFERNRLSQ FFVQDGRKIE VPPPTWPGLP NSADITPELC





DAQFRVFDDR NRFAETGGFD ALNEALTIPM VLVMSIWDDH HSNMLWLDSS YPPEKAGLPG GDRGPCPTTS





GVPAEVEAQY PNAQVVWSNI RFGPIGSTVN V


 


SEQ ID NO: 18
950686
Humicola grisea
MRTAKFATLA ALVASAAAQQ ACSLTTERHP SLSWKKCTAG GQCQTVQASI TLDSNWRWTH QVSGSTNCYT





GNKWDTSICT DAKSCAQNCC VDGADYTSTY GITTNGDSLS LKFVTKGQYS TNVGSRTYLM DGEDKYQTFE





LLGNEFTFDV DVSNIGCGLN GALYFVSMDA DGGLSRYPGN KAGAKYGTGY CDAQCPRDIK FINGEANIEG





WTGSTNDPNA GAGRYGTCCS EMDIWEANNM ATAFTPHPCT IIGQSRCEGD SCGGTYSNER YAGVCDPDGC





DFNSYRQGNK TFYGKGMTVD TTKKITVVTQ FLKDANGDLG EIKRFYVQDG KIIPNSESTI PGVEGNSITQ





DWCDRQKVAF GDIDDFNRKG GMKQMGKALA GPMVLVMSIW DDHASNMLWL DSTFPVDAAG KPGAERGACP





TTSGVPAEVE AEAPNSNVVF SNIRFGPIGS TVAGLPGAGN GGNNGGNPPP PTTTTSSAPA TTTTASAGPK





AGRWQQCGGI GFTGPTQCEE PYTCTKLNDW YSQCL


 


SEQ ID NO: 19
124491660
Chaetomium
MQIKQYLQYL AAALPLVNMA AAQRAGTQQT ETHPRLSWKR CSSGGNCQTV NAEIVIDANW RWLHDSNYQN




thermophilum
CYDGNRWTSA CSSATDCAQK CYLEGANYGS TYGVSTSGDA LTLKFVTKHE YGTNIGSRVY LMNGSDKYQM





FTLMNNEFAF DVDLSKVECG LNSALYFVAM EEDGGMRSYS SNKAGAKYGT GYCDAQCARD LKFVGGKANI





EGWRPSTNDA NAGVGPYGAC CAEIDVWESN AYAFAFTPHG CLNNNYHVCE TSNCGGTYSE DRFGGLCDAN





GCDYNPYRMG NKDFYGKGKT VDTSRKFTVV TRFEENKLTQ FFIQDGRKID IPPPTWPGLP NSSAITPELC





TNLSKVFDDR DRYEETGGFR TINEALRIPM VLVMSIWDGH YANMLWLDSV YPPEKAGQPG AERGPCAPTS





GVPAEVEAQF PNAQVIWSNI RFGPIGSTYQ V


 


SEQ ID NO: 20
58045187
Chaetomium
MMYKKFAALA ALVAGAAAQQ ACSLTTETHP RLTWKRCTSG GNCSTVNGAV TIDANWRWTH TVSGSTNCYT




thermophilum
GNEWDTSICS DGKSCAQTCC VDGADYSSTY GITTSGDSLN LKFVTKHQHG TNVGSRVYLM ENDTKYQMFE





LLGNEFTFDV DVSNLGCGLN GALYFVSMDA DGGMSKYSGN KAGAKYGTGY CDAQCPRDLK FINGEANIEN





WTPSTNDANA GFGRYGSCCS EMDIWDANNM ATAFTPHPCT IIGQSRCEGN SCGGTYSSER YAGVCDPDGC





DFNAYRQGDK TFYGKGMTVD TTKKMTVVTQ FHKNSAGVLS EIKRFYVQDG KIIANAESKI PGNPGNSITQ





EWCDAQKVAF GDIDDFNRKG GMAQMSKALE GPMVLVMSVW DDHYANMLWL DSTYPIDKAG TPGAERGACP





TTSGVPAEIE AQVPNSNVIF SNIRFGPIGS TVPGLDGSTP SNPTATVAPP TSTTTSVRSS TTQISTPTSQ





PGGCTTQKWG QCGGIGYTGC TNCVAGTTCT ELNPWYSQCL


 


SEQ ID NO: 21
169601100 #
Phaeosphaeria
MYRNFLYAAS LLSVARSQLV GTQTTETHPG MTWQSCTAKG SCTTCSDNKA CASNCAVDGA DYKGTYGITA




nodorum SN15
SGNSLQLKFI TKGSYSTNIG SRTYLMASDT AYQMFKFDGN KEFTFDVDLS GLPCGFNGAL YFVSMDEDGG





LKKYSGNKAG AKYGTGYCDA QCPRDLKFIN GEGNVEGWKP SDNDANAGVG GHGSCCAEMD IWEANSISTA





VTPHACSTIE QTRCDGDGCG GTYSADRYAG VCDPDGCDFN AYRMGVKNFY GKGMTVDTSK KFTVVTQFIG





TGDAMEIKRF YVQGGKTIEQ PASTIPGVEG NSITTKFCDQ QKQVFGDRYT YKEKGGTANM AKALAQGMVL





VMSLWDDHYS NMLWLDSTYP TDKNPDTDLG SGRGSCDVKS GAPADVESKS PDATVIYSNI KFGPLNSTY


 


SEQ ID NO: 22
169870197
Coprinopsis cinerea
MLGKIAIASL SFLAIAKGQQ VGREVAENHP RLPWQRCTRN GGCQTVSNGQ VVLDANWRWL HVTDGYTNCY




Okayama
TGNSWNSSVC SDGTTCAQRC ALEGANYQQT YGITTSGNSL TMKFLTRSQG TNVGGRVYLM ENENRYQMFN





LLNKEFTFDV DVSKVPCGIN GALYFIQMDA DGGMSSQPNN RAGAKYGTGY CDSQCPRDIK FIDGVANSVG





WEPSETDSNA GRGRYGICCA EMDIWEANSI SNAYTPHPCR TQNDGGYQRC EGRDCNQPRY EGLCDPDGCD





YNPFRMGNKD FYGPGKTIDT NRKMTVVTQF ITHDNTDTGT LVDIRRLYVQ DGRVIANPPT NFPGLMPAHD





SITEQFCTDQ KNLFGDYSSF ARDGGLAHMG RSLAKGHVLA LSIWNDHGAH MLWLDSNYPT DADPNKPGIA





RGTCPTTGGT PRETEQNHPD AQVIFSNIKF GDIGSTFSGY


 


SEQ ID NO: 23
3913806
Agaricus bisporus
MFPRSILLAL SLTAVALGQQ VGTNMAENHP SLTWQRCTSS GCQNVNGKVT LDANWRWTHR INDFTNCYTG





NEWDTSICPD GVTCAENCAL DGADYAGTYG VTSSGTALTL KFVTESQQKN IGSRLYLMAD DSNYEIFNLL





NKEFTFDVDV SKLPCGLNGA LYFSEMAADG GMSSTNTAGA KYGTGYCDSQ CPRDIKFIDG EANSEGWEGS





PNDVNAGTGN FGACCGEMDI WEANSISSAY TPHPCREPGL QRCEGNTCSV NDRYATECDP DGCDFNSFRM





GDKSFYGPGM TVDTNQPITV VTQFITDNGS DNGNLQEIRR IYVQNGQVIQ NSNVNIPGID SGNSISAEFC





DQAKEAFGDE RSFQDRGGLS GMGSALDRGM VLVLSIWDDH AVNMLWLDSD YPLDASPSQP GISRGTCSRD





SGKPEDVEAN AGGVQVVYSN IKFGDINSTF NNNGGGGGNP SPTTTRPNSP AQTMWGQCGG QGWTGPTACQ





SPSTCHVIND FYSQCF


 


SEQ ID NO: 24
169611094
Phaeosphaeria
MYRNLALASL SLFGAARAQQ AGTVTTETHP SLSWKTCTGT GGTSCTTKAG KITLDANWRW THVTTGYTNC




nodorum SN15
YDGNSWNTTA CPDGATCTKN CAVDGADYSG TYGITTSSNS LSIKFVTKGS NSANIGSRTY LMESDTKYQM





FNLIGQEFTF DVDVSKLPCG LNGALYFVEM AADGGIGKGN NKAGAKYGTG YCDSQCPHDI KFINGKANVE





GWNPSDADPN AGSGKIGACC PEMDIWEANS ISTAYTPHPC KGTGLQECTD DVSCGDGSNR YSGLCDKDGC





DFNSYRMGVK DFYGPGATLD TTKKMTVVTQ FLGSGSTLSE IKRFYVQNGK VFKNSDSAIE GVTGNSITES





FCAAQKTAFG DTNSFKTLGG LNEMGASLAR GHVLVMSLWD DHAVNMLWLD STYPTNSTKL GAQRGTCAID





SGKPEDVEKN HPDATVVFSD IKFGPIGSTF QQPS


 


SEQ ID NO: 25
3131
Phanerochaete
MVDIQIATFL LLGVVGVAAQ QVGTYIPENH PLLATQSCTA SGGCTTSSSK IVLDANRRWI HSTLGTTSCL




chrysosporium
TANGWDPTLC PDGITCANYC ALDGVSYSST YGITTSGSAL RLQFVTGTNI GSRVFLMADD THYRTFQLLN





QELAFDVDVS KLPCGLNGAL YFVAMDADGG KSKYPGNRAG AKYGTGYCDS QCPRDVQFIN GQANVQGWNA





TSATTGTGSY GSCCTELDIW EANSNAAALT PHTCTNNAQT RCSGSNCTSN TGFCDADGCD FNSFRLGNTT





FLGAGMSVDT TKTFTVVTQF ITSDNTSTGN LTEIRRFYVQ NGNVIPNSVV NVTGIGAVNS ITDPFCSQQK





KAFIETNYFA QHGGLAQLGQ ALRTGMVLAF SISDDPANHM LWLDSNFPPS ANPAVPGVAR GMCSITSGNP





ADVGILNPSP YVSFLNIKFG SIGTTFRPA


 


SEQ ID NO: 26
70991503
Aspergillus
MHQRALLFSA LAVAANAQQV GTQTPETHPP LTWQKCTAAG SCSQQSGSVV IDANWRWLHS TKDTTNCYTG




fumigatus Af293
NTWNTELCPD NESCAQNCAL DGADYAGTYG VTTSGSELKL SFVTGANVGS RLYLMQDDET YQHFNLLNHE





FTFDVDVSNL PCGLNGALYF VAMDADGGMS KYPSNKAGAK YGTGYCDSQC PRDLKFINGM ANVEGWEPSS





SDKNAGVGGH GSCCPEMDIW EANSISTAVT PHPCDDVSQT MCSGDACGGT YSESRYAGTC DPDGCDFNPF





RMGNESFYGP GKIVDTKSKM TVVTQFITAD GTDSGALSEI KRLYVQNGKV IANSVSNVAG VSGNSITSDF





CTAQKKAFGD EDIFAKHGGL SGMGKALSEM VLIMSIWDDH HSSMMWLDST YPTDADPSKP GVARGTCEHG





AGDPENVESQ HPDASVTFSN IKFGPIGSTY EG


 


SEQ ID NO: 27
294196
Phanerochaete
MFRTATLLAF TMAAMVFGQQ VGTNTAENHR TLTSQKCTKS GGCSNLNTKI VLDANWRWLH STSGYTNCYT




chrysosporium
GNQWDATLCP DGKTCAANCA LDGADYTGTY GITASGSSLK LQFVTGSNVG SRVYLMADDT HYQMFQLLNQ





EFTFDVDMSN LPCGLNGALY LSAMDADGGM AKYPTNKAGA KYGTGYCDSQ CPRDIKFING EANVEGWNAT





SANAGTGNYG TCCTEMDIWE ANNDAAAYTP HPCTTNAQTR CSGSDCTRDT GLCDADGCDF NSFRMGDQTF





LGKGLTVDTS KPFTVVTQFI TNDGTSAGTL TEIRRLYVQN GKVIQNSSVK IPGIDPVNSI TDNFCSQQKT





AFGDTNYFAQ HGGLKQVGEA LRTGMVLALS IWDDYAANML WLDSNYPTNK DPSTPGVARG TCATTSGVPA





QIEAQSPNAY VVFSNIKFGD LNTTYTGTVS SSSVSSSHSS TSTSSSHSSS STPPTQPTGV TVPQWGQCGG





IGYTGSTTCA SPYTCHVLNP YYSQCY


 


SEQ ID NO: 28
18997123
Thermoascus
MYQRALLFSF FLAAARAHEA GTVTAENHPS LTWQQCSSGG SCTTQNGKVV IDANWRWVHT TSGYTNCYTG




aurantiacus
NTWDTSICPD DVTCAQNCAL DGADYSGTYG VTTSGNALRL NFVTQSSGKN IGSRLYLLQD DTTYQIFKLL





GQEFTFDVDV SNLPCGLNGA LYFVAMDADG NLSKYPGNKA GAKYGTGYCD SQCPRDLKFI NGQANVEGWQ





PSANDPNAGV GNHGSSCAEM DVWEANSIST AVTPHPCDTP GQTMCQGDDC GGTYSSTRYA GTCDPDGCDF





NPYQPGNHSF YGPGKIVDTS SKFTVVTQFI TDDGTPSGTL TEIKRFYVQN GKVIPQSEST ISGVTGNSIT





TEYCTAQKAA FGDNTGFFTH GGLQKISQAL AQGMVLVMSL WDDHAANMLW LDSTYPTDAD PDTPGVARGT





CPTTSGVPAD VESQNPNSYV IYSNIKVGPI NSTFTAN


 


SEQ ID NO: 29
4204214
Humicola grisea var
MQIKSYIQYL AAALPLLSSV AAQQAGTITA ENHPRMTWKR CSGPGNCQTV QGEVVIDANW RWLHNNGQNC




thermoidea
YEGNKWTSQC SSATDCAQRC ALDGANYQST YGASTSGDSL TLKFVTKHEY GTNIGSRFYL MANQNKYQMF





TLMNNEFAFD VDLSKVECGI NSALYFVAME EDGGMASYPS NRAGAKYGTG YCDAQCARDL KFIGGKANIE





GWRPSTNDPN AGVGPMGACC AEIDVWESNA YAYAFTPHAC GSKNRYHICE TNNCGGTYSD DRFAGYCDAN





GCDYNPYRMG NKDFYGKGKT VDTNRKFTVV SRFERNRLSQ FFVQDGRKIE VPPPTWPGLP NSADITPELC





DAQFRVFDDR NRFAETGGFD ALNEALTIPM VLVMSIWDDH HSNMLWLDSS YPPEKAGLPG GDRGPCPTTS





GVPAEVEAQY PDAQVVWSNI RFGPIGSTVN V


 


SEQ ID NO: 30
34582632
Trichoderma viride
MYRKLAVISA FLATARAQSA CTLQSETHPP LTWQKCSSGG TCTQQTGSVV IDANWRWTHA TNSSTNCYDG




(also known as
NTWSSTLCPD NETCAKNCCL DGAAYASTYG VTTSGNSLSI GFVTQSAQKN VGARLYLMAS DTTYQEFTLL




Hypochrea rufa)
GNEFSFDVDV SQLPCGLNGA LYFVSMDADG GVSKYPTNTA GAKYGTGYCD SQCPRDLKFI NGQANVEGWE





PSSNNANTGI GGHGSCCSEM DIWEANSISE ALTPHPCTTV GQEICEGDGC GGTYSDNRYG GTCDPDGCDW





DPYRLGNTSF YGPGSSFTLD TTKKLTVVTQ FETSGAINRY YVQNGVTFQQ PNAELGSYSG NGLNDDYCTA





EEAEFGGSSF SDKGGLTQFK KATSGGMVLV MSLWDDYYAN MLWLDSTYPT NETSSTPGAV RGSCSTSSGV





PAQVESQSPN AKVTFSNIKF GPIGSTGDPS GGNPPGGNPP GTTTTRRPAT TTGSSPGPTQ SHYGQCGGIG





YSGPTVCASG TTCQVLNPYY SQCL


 


SEQ ID NO: 31
156712284
Thermoascus
MYQRALLFSF FLAAARAQQA GTVTAENHPS LTWQQCSSGG SCTTQNGKVV IDANWRWVHT TSGYTNCYTG




aurantiacus
NTWDTSICPD DVTCAQNCAL DGADYSGTYG VTTSGNALRL NFVTQSSGKN IGSRLYLLQD DTTYQIFKLL





GQEFTFDVDV SNLPCGLNGA LYFVAMDADG GLSKYPGNKA GAKYGTGYCD SQCPRDLKFI NGQANVEGWQ





PSANDPNAGV GNHGSCCAEM DVWEANSIST AVTPHPCDTP GQTMCQGDDC GGTYSSTRYA GTCDPDGCDF





NPYRQGNHSF YGPGQIVDTS SKFTVVTQFI TDDGTPSGTL TEIKRFYVQN GKVIPQSEST ISGVTGNSIT





TEYCTAQKAA FGDNTGFFTH GGLQKISQAL AQGMVLVMSL WDDHAANMLW LDSTYPTDAD PDTPGVARGT





CPTTSGVPAD VESQYPNSYV IYSNIKVGPI NSTFTAN


 


SEQ ID NO: 32
39977899
Magnaporthe grisea
MIRKITTLAA LVGVVRGQAA CSLTAETHPS LTWQKCSSGG SCTNVAGSVT IDANWRWTHT TSGYTNCYTG




(oryzae) 70-15
NKWDTSICST NADCASKCCV DGANYQQTYG ASTSGNALSL QYVTQSSGKN VGSRLYLLES ENKYQMFNLL





GNEFTFDVDA SKLGCGLNGA VYFVSMDADG GQSKYSGNKA GAKYGTGYCD SQCPRDLKYI NGAANVEGWQ





PSSGDANSGV GNMGSCCAEM DIWEANSIST AYTPHPCSNN AQHSCKGDDC GGTYSSVRYA GDCDPDGCDF





NSYRQGNRTF YGPGSNFNVD SSKKVTVVTQ FISSGGQLTD IKRFYVQNGK VIPNSQSTIT GVTGNSVTQD





YCDKQKTAFG DQNVFNQRGG LRQMGDALAK GMVLVMSVWD DHHSQMLWLD STYPTTSTAP GAARGSCSTS





SGKPSDVQSQ TPGATVVYSN IKFGPIGSTF KSS


 


SEQ ID NO: 33
20986705
Talaromyces
MLRRALLLSS SAILAVKAQQ AGTATAENHP PLTWQECTAP GSCTTQNGAV VLDANWRWVH DVNGYTNCYT




emersonii
GNTWDPTYCP DDETCAQNCA LDGADYEGTY GVTSSGSSLK LNFVTGSNVG SRLYLLQDDS TYQIFKLLNR





EFSFDVDVSN LPCGLNGALY FVAMDADGGV SKYPNNKAGA KYGTGYCDSQ CPRDLKFIDG EANVEGWQPS





SNNANTGIGD HGSCCAEMDV WEANSISNAV TPHPCDTPGQ TMCSGDDCGG TYSNDRYAGT CDPDGCDFNP





YRMGNTSFYG PGKIIDTTKP FTVVTQFLTD DGTDTGTLSE IKRFYIQNSN VIPQPNSDIS GVTGNSITTE





FCTAQKQAFG DTDDFSQHGG LAKMGAAMQQ GMVLVMSLWD DYAAQMLWLD SDYPTDADPT TPGIARGTCP





TDSGVPSDVE SQSPNSYVTY SNIKFGPINS TFTAS


 


SEQ ID NO: 34
22138843
Aspergillus oryzae
MHQRALLFSA FWTAVQAQQA GTLTAETHPS LTWQKCAAGG TCTEQKGSVV LDSNWRWLHS VDGSTNCYTG





NTWDATLCPD NESCASNCAL DGADYEGTYG VTTSGDALTL QFVTGANIGS RLYLMADDDE SYQTFNLLNN





EFTFDVDASK LPCGLNGAVY FVSMDADGGV AKYSTNKAGA KYGTGYCDSQ CPRDLKFING QVRKGWEPSD





SDKNAGVGGH GSCCPQMDIW EANSISTAYT PHPCDDTAQT MCEGDTCGGT YSSERYAGTC DPDGCDFNAY





RMGNESFYGP SKLVDSSSPV TVVTQFITAD GTDSGALSEI KRFYVQGGKV IANAASNVDG VTGNSITADF





CTAQKKAFGD DDIFAQHGGL QGMGNALSSM VLTLSIWDDH HSSMMWLDSS YPEDADATAP GVARGTCEPH





AGDPEKVESQ SGSATVTYSN IKYGPIGSTF DAPA


 


SEQ ID NO: 35
55775695
Penicillium
MASTLSFKIY KNALLLAAFL GAAQAQQVGT STAEVHPSLT WQKCTAGGSC TSQSGKVVID SNWRWVHNTG




chrysogenum
GYTNCYTGND WDRTLCPDDV TCATNCALDG ADYKGTYGVT ASGSSLRLNF VTQASQKNIG SRLYLMADDS





KYEMFQLLNQ EFTFDVDVSN LPCGLNGALY FVAMDEDGGM ARYPTNKAGA KYGTGYCDAQ CPRDLKFING





QANVEGWEPS SSDVNGGTGN YGSCCAEMDI WEANSISTAF TPHPCDDPAQ TRCTGDSCGG TYSSDRYGGT





CDPDGCDFNP YRMGNQSFYG PSKIVDTESP FTVVTQFITN DGTSTGTLSE IKRFYVQNGK VIPQSVSTIS





AVTGNSITDS FCSAQKTAFK DTDVFAKHGG MAGMGAGLAE GMVLVMSLWD DHAANMLWLD STYPTSASST





TPGAARGSCD ISSGEPSDVE ANHSNAYVVY SNIKVGPLGS TFGSTDSGSG TTTTKVTTTT ATKTTTTTGP





STTGAAHYAQ CGGQNWTGPT TCASPYTCQR QGDYYSQCL


 


SEQ ID NO: 36
171676762
Podospora anserina
MVSAKFAALA ALVASASAQQ VCSLTPESHP PLTWQRCSAG GSCTNVAGSV TLDSNWRWTH TLQGSTNCYS





GNEWDTSICT TGTKCAQNCC VEGAEYAATY GITTSGNQLN LKFVTEGKYS TNVGSRTYLM ENATKYQGFN





LLGNEFTFDV DVSNIGCGLN GALYFVSMDL DGGLAKYSGN KAGAKYGTGY CDAQCPRDIK FINGEANIEG





WNPSTNDVNA GAGRYGTCCS EMDIWEANNM ATAYTPHSCT ILDQSRCEGE SCGGTYSSDR YGGVCDPDGC





DFNSYRMGNK EFYGKGKTVD TTKKMTVVTQ FLKNAAGELS EIKRFYVQNG VVIPNSVSSI PGVPNQNSIT





QDWCDAQKIA FGDPDDNTAK GGLRQMGLAL DKPMVLVMSI WNDHAAHMLW LDSTYPVDAA GRPGAERGAC





PTTSGVPSEV EAEAPNSNVA FSNIKFGPIG STFNSGSTNP NPISSSTATT PTSTRVSSTS TAAQTPTSAP





GGTVPRWGQC GGQGYTGPTQ CVAPYTCVVS NQWYSQCL


 


SEQ ID NO: 37
146350520
Pleurotus sp Florida
MFPYIALVSF SFLSVVLAQQ VGTLTAETHP QLTVQQCTRG GSCTTQQRSV VLDGNWRWLH STSGSNNCYT





GNTWDTSLCP DAATCSRNCA LDGADYSGTY GITSSGNALT LKFVTHGPYS TNIGSRVYLL ADDSHYQMFN





LKNKEFTFDV DVSQLPCGLN GALYFSQMDA DGGTGRFPNN KAGAKYGTGY CDSQCPHDIK FINGEANVQG





WQPSPNDSNA GKGQYGSCCA EMDIWEANSM ASAYTPHPCT VTTPTRCQGN DCGDGDNRYG GVCDKDGCDF





NSFRMGDKNF LGPGKTVNTN SKFTVVTQFL TSDNTTSGTL SEIRRLYVQN GRVIQNSKVN IPGMASTLDS





ITESFCSTQK TVFGDTNSFA SKGGLRAMGN AFDKGMVLVL SIWDDHEAKM LWLDSNYPLD KSASAPGVAR





GTCATTSGEP KDVESQSPNA QVIFSNIKYG DIGSTYSN


 


SEQ ID NO: 38
37732123
Gibberella zeae
MYRAIATASA LIAAVRAQQV CSLTQESKPS LNWSKCTSSG CSNVKGSVTI DANWRWTHQV SGSTNCYTGN





KWDTSVCTSG KVCAERCCLD GADYASTYGI TSSGDQLSLS FVTKGPYSTN IGSRTYLMED ENTYQMFQLL





GNEFTFDVDV SNIGCGLNGA LYFVSMDADG GKAKYPGNKA GAKYGTGYCD AQCPRDVKFI NGQANSDGWQ





PSDSDVNGGI GNLGTCCPEM DIWEANSIST AYTPHPCTKL TQHSCTGDSC GGTYSNDRYG GTCDADGCDF





NSYRQGNKTF YGPGSGFNVD TTKKVTVVTQ FHKGSNGRLS EITRLYVQNG KVIANSESKI AGVPGNSLTA





DFCTKQKKVF NDPDDFTKKG AWSGMSDALE APMVLVMSLW HDHHSNMLWL DSTYPTDSTK LGSQRGSCST





SSGVPADLEK NVPNSKVAFS NIKFGPIGST YKSDGTTPTN PTNPSEPSNT ANPNPGTVDQ WGQCGGSNYS





GPTACKSGFT CKKINDFYSQ CQ


 


SEQ ID NO: 39
156055188
Sclerotinia
MYSAAVLATF SFLLGAGAQQ VGTLKTESHP PLTIQKCAAG GTCTDEADSV VLDANWRWLH STSGSTNCYT




sclerotiorum 1980
GNTWDTTLCP DAATCTANCA FDGADYEGTY GITSSGDSLK LSFVTGSNVG SRTYLMDSET TYKEFALLGN





EFTFTVDVSK LPCGLNGALY FVPMDADGGM SKYPTNKAGA KYGTGYCDAQ CPQDMKFVSG GANNEGWVPD





SNSANSGTGN IGSCCSEFDV WEANSMSQAL TPHTCTVDGQ TACTGDDCAG NTGVCDADGC DFNPYRMGNT





TFYGSGKTID TTKPFSVVTQ FITDDGTETG TLTEIKRFYV QDDVVYEQPN SDISGVSGNS ITDDFCTAQK





TAFGDTDYFS QKGGMAAMGK KMADGMVLVL SIWDDYNVNM LWLDSDYPTT KDASTPGVSR GSCATTSGVP





ATVEAASGSA YVTFSSIKYG PIGSTFKAPA DSSSPVVASS SPAAVAAVVS TSSAQAVPSH PAVSSSQAAV





STPEAVSSAP EVPASSSAAQ SVAPTSTKPK CSKVSQSSTL ATSVAAPATT ATSAAVAATS AASSSGSVPL





YGNCTGGKTC SEGTCVVQNP WYSQCVASS


 


SEQ ID NO: 40
453224
Phanerochaete
MFRAAALLAF TCLAMVSGQQ AGTNTAENHP QLQSQQCTTS GGCKPLSTKV VLDSNWRWVH STSGYTNCYT




chrysosporium
GNEWDTSLCP DGKTCAANCA LDGADYSGTY GITSTGTALT LKFVTGSNVG SRVYLMADDT HYQLLKLLNQ





EFTFDVDMSN LPCGLNGALY LSAMDADGGM SKYPGNKAGA KYGTGYCDSQ CPKDIKFING EANVGNWTET





GSNTGTGSYG TCCSEMDIWE ANNDAAAFTP HPCTTTGQTR CSGDDCARNT GLCDGDGCDF NSFRMGDKTF





LGKGMTVDTS KPFTVVTQFL TNDNTSTGTL SEIRRIYIQN GKVIQNSVAN IPGVDPVNSI TDNFCAQQKT





AFGDTNWFAQ KGGLKQMGEA LGNGMVLALS IWDDHAANML WLDSDYPTDK DPSAPGVARG TCATTSGVPS





DVESQVPNSQ VVFSNIKFGD IGSTFSGTSS PNPPGGSTTS SPVTTSPTPP PTGPTVPQWG QCGGIGYSGS





TTCASPYTCH VLNPYYSQCY


 


SEQ ID NO: 41
50402144
Trichoderma reesei
MYRKLAVISA FLATARAQSA CTLQSETHPP LTWQKCSSGG TCTQQTGSVV IDANWRWTHA TNSSTNCYDG





NTWSSTLCPD NETCAKNCCL DGAAYASTYG VTTSGNSLSI GFVTQSAQKN VGARLYLMAS DTTYQEFTLL





GNEFSFDVDV SQLPCGLNGA LYFVSMDADG GVSKYPTNTA GAKYGTGYCD SQCPRDLKFI NGQANVEGWE





PSSNNANTGI GGHGSCCSEM DIWEANSISE ALTPHPCTTV GQEICEGDGC GGTYSDNRYG GTCDPDGCDW





NPYRLGNTSF YGPGSSFTLD TTKKLTVVTQ FETSGAINRY YVQNGVTFQQ PNAELGSYSG NELNDDYCTA





EEAEFGGSSF SDKGGLTQFK KATSGGMVLV MSLWDDYYAN MLWLDSTYPT NETSSTPGAV RGSCSTSSGV





PAQVESQSPN AKVTFSNIKF GPIGSTGNPS GGNPPGGNRG TTTTRRPATT TGSSPGPTQS HYGQCGGIGY





SGPTVCASGT TCQVLNPYYS QCL


 


SEQ ID NO: 42
115397177
Aspergillus terreus
MPSTYDIYKK LLLLASFLSA SQAQQVGTSK AEVHPSLTWQ TCTSGGSCTT VNGKVVVDAN WRWVHNVDGY




NIH2624
NNCYTGNTWD TTLCPDDETC ASNCALEGAD YSGTYGVTTS GNSLRLNFVT QASQKNIGSR LYLMEDDSTY





KMFKLLNQEF TFDVDVSNLP CGLNGAVYFV SMDADGGMAK YPANKAGAKY GTGYCDSQCP RDLKFINGMA





NVEGWEPSAN DANAGTGNHG SCCAEMDIWE ANSISTAYTP HPCDTPGQVM CTGDSCGGTY SSDRYGGTCD





PDGCDFNSYR QGNKTFYGPG MTVDTKSKIT VVTQFLTNDG TASGTLSEIK RFYVQNGKVI PNSESTWSGV





SGNSITTAYC NAQKTLFGDT DVFTKHGGME GMGAALAEGM VLVLSLWDDH NSNMLWLDSN YPTDKPSTTP





GVARGSCDIS SGDPKDVEAN DANAYVVYSN IKVGPIGSTF SGSTGGGSSS STTATSKTTT TSATKTTTTT





TKTTTTTSAS STSTGGAQHW AQCGGIGWTG PTTCVAPYTC QKQNDYYSQC L


 


SEQ ID NO: 43
154312003
Botryotinia
MISKVLAFTS LLAAARAQQA GTLTTETHPP LSVSQCTASG CTTSAQSIVV DANWRWLHST TGSTNCYTGN




fuckeliana B05-10
TWDKTLCPDG ATCAANCALD GADYSGVYGI TTSGNSIKLN FVTKGANTNV GSRTYLMAAG STTQYQMLKL





LNQEFTFDVD VSNLPCGLNG ALYFAAMDAD GGLSRFPTNK AGAKYGTGYC DAQCPQDIKF INGVANSVGW





TPSSNDVNAG AGQYGSCCSE MDIWEANKIS AAYTPHPCSV DTQTRCTGTD CGIGARYSSL CDADGCDFNS





YRQGNTSFYG AGLTVNTNKV FTVVTQFITN DGTASGTLKE IRRFYVQNGV VIPNSQSTIA GVPGNSITDS





FCAAQKTAFG DTNEFATKGG LATMSKALAK GMVLVMSIWD DHTANMLWLD APYPATKSPS APGVTRGSCS





ATSGNPVDVE ANSPGSSVTF SNIKWGPINS TYTGSGAAPS VPGTTTVSSA PASTATSGAG GVAKYAQCGG





SGYSGATACV SGSTCVALNP YYSQCQ


 


SEQ ID NO: 44
49333365
Volvariella volvacea
MFPAATLFAF SLFAAVYGQQ VGTQLAETHP RLTWQKCTRS GGCQTQSNGA IVLDANWRWV HNVGGYTNCY





TGNTWNTSLC PDGATCAKNC ALDGANYQST YGITTSGNAL TLKFVTQSEQ KNIGSRVYLL ESDTKYQLFN





PLNQEFTFDV DVSQLPCGLN GAVYFSAMDA DGGMSKFPNN AAGAKYGTGY CDSQCPRDIK FINGEANVQG





WQPSPNDTNA GTGNYGACCN EMDVWEANSI STAYTPHPCT QQGLVRCSGT ACGGGSNRYG SICDPDGCDF





NSFRMGDKSF YGPGLTVNTQ QKFTVVTQFL TNNNSSSGTL REIRRLYVQN GRVIQNSKVN IPGMPSTMDS





VTTEFCNAQK TAFNDTFSFQ QKGGMANMSE ALRRGMVLVL SIWDDHAANM LWLDSNYPTD RPASQPGVAR





GTCPTSSGKP SDVENSTANS QVIYSNIKFG DIGSTYSA


 


SEQ ID NO: 45
729650
Penicillium
MKGSISYQIY KGALLLSALL NSVSAQQVGT LTAETHPALT WSKCTAGXCS QVSGSVVIDA NWPXVHSTSG




janthinellum
STNCYTGNTW DATLCPDDVT CAANCAVDGA RRQHLRVTTS GNSLRINFVT TASQKNIGSR LYLLENDTTY





QKFNLLNQEF TFDVDVSNLP CGLNGALYFV DMDADGGMAK YPTNKAGAKY GTGYCDSQCP RDLKFINGQA





NVDGWTPSKN DVNSGIGNHG SCCAEMDIWE ANSISNAVTP HPCDTPSQTM CTGQRCGGTY STDRYGGTCD





PDGCDFNPYR MGVTNFYGPG ETIDTKSPFT VVTQFLTNDG TSTGTLSEIK RFYVQGGKVI GNPQSTIVGV





SGNSITDSWC NAQKSAFGDT NEFSKHGGMA GMGAGLADGM VLVMSLWDDH ASDMLWLDST YPTNATSTTP





GAKRGTCDIS RRPNTVESTY PNAYVIYSNI KTGPLNSTFT GGTTSSSSTT TTTSKSTSTS SSSKTTTTVT





TTTTSSGSSG TGARDWAQCG GNGWTGPTTC VSPYTCTKQN DWYSQCL


 


SEQ ID NO: 46
146424871
Pleurotus sp Florida
MFRTAALTAF TLAAVVLGQQ VGTLTAENHP ALSIQQCTAS GCTTQQKSVV LDSNWRWTHS LPVHTNCYTG





NAWDASLCPD PTTCATNCAI DGADYSGTYG ITTSGNALTL RFVTNGPYSK NIGSRVYLLD DADHYKMFDL





KNQEFTFDVD MSGLPCGLNG ALYFSEMPAD GGKAAHTSNK AGAKYGTGYC DAQCPHDIKW INGEANILDW





SASATDANAG NGRYGACCAE MDIWEANSEA TAYTPHVCRD EGLYRCSGTE CGDGDNRYGG VCDKDGCDFN





SYRMGDKNFL GRGKTIDTTK KITVVTQFIT DDNTSSGNLV EIRRVYVQDG VTYQNSFSTF PSLSQYNSIS





DDFCVAQKTL FGDNQYYNTH GGTEKMGDAM ANGMVLIMSL WSDHAAHMLW LDSDYPLDKS PSEPGVSRGA





CATTTGDPDD VVANHPNASV TFSNIKYGPI GSTYGGSTPP VSSGNTSAPP VTSTTSSGPT TPTGPTGTVP





KWGQCGGNGY SGPTTCVAGS TCTYSNDWYS QCL


 


SEQ ID NO: 47
67538012
Aspergillus nidulans
MYQRALLFSA LLSVSRAQQA GTAQEEVHPS LTWQRCEASG SCTEVAGSVV LDSNWRWTHS VDGYTNCYTG




FGSC A4
NEWDATLCPD NESCAQNCAV DGADYEATYG ITSNGDSLTL KFVTGSNVGS RVYLMEDDET YQMFDLLNNE





FTFDVDVSNL PCGLNGALYF TSMDADGGLS KYEGNTAGAK YGTGYCDSQC PRDIKFINGL GNVEGWEPSD





SDANAGVGGM GTCCPEMDIW EANSISTAYT PHPCDSVEQT MCEGDSCGGT YSDDRYGGTC DPDGCDFNSY





RMGNTSFYGP GAIIDTSSKF TVVTQFIADG GSLSEIKRFY VQNGEVIPNS ESNISGVEGN SITSEFCTAQ





KTAFGDEDIF AQHGGLSAMG DAASAMVLIL SIWDDHHSSM MWLDSSYPTD ADPSQPGVAR GTCEQGAGDP





DVVESEHADA SVTFSNIKFG PIGSTF


 


SEQ ID NO: 48
62006162
Fusarium poae
MYRAIATASA LIAAVRAQQV CSLTTETKPA LTWSKCTSSG CSNVQGSVTI DANWRWTHQV SGSTNCHTGN





KWDTSVCTSG KVCAEKCCVD GADYASTYGI TSSGNQLSLS FVTKGSYGTN IGSRTYLMED ENTYQMFQLL





GNEFTFDVDV SNIGCGLNGA LYFVSMDADG GKAKYPGNKA GAKYGTGYCD AQCPRDVKFI NGQANSDGWE





PSKSDVNGGI GNLGTCCPEM DIWEANSIST AYTPHPCTKL TQHACTGDSC GGTYSNDRYG GTCDADGCDF





NAYRQGNKTF YGPGSGFNVD TTKKVTVVTQ FHKGSNGRLS EITRLYVQNG KVIANSESKI AGNPGSSLTS





DFCTTQKKVF GDIDDFAKKG AWNGMSDALE APMVLVMSLW HDHHSNMLWL DSTYPTDSTA LGSQRGSCST





SSGVPADLEK NVPNSKVAFS NIKFGPIGST YNKEGTQPQP TNPTNPNPTN PTNPGTVDQW GQCGGTNYSG





PTACKSPFTC KKINDFYSQC Q


 


SEQ ID NO: 49
146424873
Pleurotus sp Florida
MFRTAALTAF TLAAVVLGQQ VGTLAAENHP ALSIQQCTAS GCTTQQKSVV LDSNWRWTHS TAGATNCYTG





NAWDSSLCPN PTTCATNCAI DGADYSGTYG ITTSGNSLTL RFVTNGQYSE NIGSRVYLLD DADHYKLFNL





KNQEFTFDVD MSGLPCGLNG ALYFSEMAAD GGKAAHTGNN AGAKYGTGYC DAQCPHDIKW INGEANILDW





SGSATDPNAG NGRYGACCAE MDIWEANSEA TAYTPHVCRD EGLYRCSGTE CGDGDNRYGG VCDKDGCDFN





SYRMGDKNFL GRGKTIDTTK KITVVTQFIT DDNTPTGNLV EIRRVYVQDG VTYQNSFSTF PSLSQYNSIS





DDFCVAQKTL FGDNQYYNTH GGTEKMGDSL ANGMVLIMSL WSDHAAHMLW LDSDYPLDKS PSEPGVSRGA





CATTTGDPDD VVANHPNASV TFSNIKYGPI GSTYGGSTPP VSSGNTSVPP VTSTTSSGPT TPTGPTGTVP





KWGQCGGIGY SGPTSCVAGS TCTYSNEWYS QCL


 


SEQ ID NO: 50
295937
Trichoderma viride
MYQKLALISA FLATARAQSA CTLQAETHPP LTWQKCSSGG TCTQQTGSVV IDANWRWTHA TNSSTNCYDG





NTWSSTLCPD NETCAKNCCL DGAAYASTYG VTTSADSLSI GFVTQSAQKN VGARLYLMAS DTTYQEFTLL





GNEFSFDVDV SQLPCGLNGA LYFVSMDADG GVTKYPTNTA GAKYGTGYCD SQCPRDLKFI NGQANVEGWE





PSSNNANTGI GGHGSCCSEM DIWEANSISE ALTPHPCTTV GQEICEGDSC GGTYSGDRYG GTCDPDGCDW





NPYRLGNTSF YGPGSSFTLD TTKKLTVVTQ FETSGAINRY YVQNGVTFQQ PNAELGDYSG NSLDDDYCAA





EEAEFGGSSF SDKGGLTQFK KATSGGMVLV MSLWDDYYAN MLWLDSTYPT DETSSTPGAV RGSSSTSSGV





PAQLESNSPN AKVVYSNIKF GPIGSTGNPS GGNPPGGNPP GTTTPRPATS TGSSPGPTQT HYGQCGGIGY





IGPTVCASGS TCQVLNPYYS QCL


 


SEQ ID NO: 51
6179889 #
Alternaria alternata
MTWQSCTAKG SCTNKNGKIV IDANWRWLHK KEGYDNCYTG NEWDATACPD NKACAANCAV DGADYSGTYG





ITAGSNSLKL KFITKGSYST NIGSRTYLMK DDTTYEMFKF TGNQEFTFDV DVSNLPCGFN GALYFVSMDA





DGGLKKYSTN KAGAKYGTGY CDAQCPRDLK FINGEGNVEG WKPSSNDANA GVGGHGSCCA EMDIWEANSV





STAVTPHSCS TIEQSRCDGD GCGGTYSADR YAGVCDPDGC DFNSYRMGVK DFYGKGKTVD TSKKFTVVTQ





FIGTGDAMEI KRFYVQNGKT IAQPASAVPG VEGNSITTKF CDQQKAVFGD TYTFKDKGGM ANMAKALANG





MVLVMSLWDD HYSNMLWLDS TYPTDKNPDT DLGTGRGECE TSSGVPADVE SQHADATVVY SNIKFGPLNS TFG


 


SEQ ID NO: 52
119483864
Neosartorya fischeri
MASAISFQVY RSALILSAFL PSITQAQQIG TYTTETHPSM TWETCTSGGS CATNQGSVVM DANWRWVHQV




NRRL 181
GSTTNCYTGN TWDTSICDTD ETCATECAVD GADYESTYGV TTSGSQIRLN FVTQNSNGAN VGSRLYMMAD





NTHYQMFKLL NQEFTFDVDV SNLPCGLNGA LYFVTMDEDG GVSKYPNNKA GAQYGVGYCD SQCPRDLKFI





QGQANVEGWT PSSNNENTGL GNYGSCCAEL DIWESNSISQ ALTPHPCDTA TNTMCTGDAC GGTYSSDRYA





GTCDPDGCDF NPYRMGNTTF YGPGKTIDTN SPFTVVTQFI TDDGTDTGTL SEIRRYYVQN GVTYAQPDSD





ISGITGNAIN ADYCTAENTV FDGPGTFAKH GGFSAMSEAM STGMVLVMSL WDDYYADMLW LDSTYPTNAS





SSTPGAVRGS CSTDSGVPAT IESESPDSYV TYSNIKVGPI GSTFSSGSGS GSSGSGSSGS ASTSTTSTKT





TAATSTSTAV AQHYSQCGGQ DWTGPTTCVS PYTCQVQNAY YSQCL


 


SEQ ID NO: 53
85083281
Neurospora crassa
MKAYFEYLVA ALPLLGLATA QQVGKQTTET HPKLSWKKCT GKANCNTVNA EVVIDSNWRW LHDSSGKNCY




OR74A
DGNKWTSACS SATDCASKCQ LDGANYGTTY GASTSGDALT LKFVTKHEYG TNIGSRFYLM NGASKYQMFT





LMNNEFAFDV DLSTVECGLN AALYFVAMEE DGGMASYSSN KAGAKYGTGY CDAQCARDLK FVGGKANIEG





WTPSTNDANA GVGPYGGCCA EIDVWESNAH SFAFTPHACK TNKYHVCERD NCGGTYSEDR FAGLCDANGC





DYNPYRMGNT DFYGKGKTVD TSKKFTVVSR FEENKLTQFF VQNGQKIEIP GPKWDGIPSD NANITPEFCS





AQFQAFGDRD RFAEVGGFAQ LNSALRMPMV LVMSIWDDHY ANMLWLDSVY PPEKEGQPGA ARGDCPQSSG





VPAEVESQYA NSKVVYSNIR FGPVGSTVNV


 


SEQ ID NO: 54
3913803
Cryphonectria
MFSKFALTGS LLAGAVNAQG VGTQQTETHP QMTWQSCTSP SSCTTNQGEV VIDSNWRWVH DKDGYVNCYT




parasitica
GNTWNTTLCP DDKTCAANCV LDGADYSSTY GITTSGNALS LQFVTQSSGK NIGSRTYLME SSTKYHLFDL





IGNEFAFDVD LSKLPCGLNG ALYFVTMDAD GGMAKYSTNT AGAEYGTGYC DSQCPRDLKF INGQGNVEGW





TPSTNDANAG VGGLGSCCSE MDVWEANSMD MAYTPHPCET AAQHSCNADE CGGTYSSSRY AGDCDPDGCD





WNPFRMGNKD FYGSGDTVDT SQKFTVVTQF HGSGSSLTEI SQYYIQGGTK IQQPNSTWPT LTGYNSITDD





FCKAQKVEFN DTDVFSEKGG LAQMGAGMAD GMVLVMSLWD DHYANMLWLD STYPVDADAS SPGKQRGTCA





TTSGVPADVE SSDASATVIY SNIKFGPIGA TY


 


SEQ ID NO: 55
60729633
Corticium rolfsii
MFPAAALLSF TLLAVASAQQ IGTNTAEVHP SLTVSQCTTS GGCTSSTQSI VLDANWRWLH STSGYTNCYT





GNQWNSDLCP DPDTCATNCA LDGASYESTY GISTDGNAVT LNFVTQGSQT NVGSRVYLLS DDTHYQTFSL





LNKEFSFDVD ASNIGCGING AVYFVQMDAD GGLSKYSSNK AGAQYGTGYC DSQCPQDIKF INGEANLLDW





NATSANSGTG SYGSCCPEMD IWEANKYAAA YTPHPCSVSG QTRCTGTSCG AGSERYDGYC DKDGCDFNSW





RMGNETFLGP GMTIDTNKKF TIVTQFITDD NTANGTLSEI RRLYVQGGTV IQNSVANQPN IPKVNSITDS





FCTAQKTEFG DQDYFGTIGG LSQMGKAMSD MVLVMSIWDD YDAEMLWLDS NYPTSGSAST PGISRGPCSA





TSGLPATVES QQASASVTYS NIKWGDIGST YSGSGSSGSS SSSSSSAASA STSTHTSAAA TATSSAAAAT





GSPVPAYGQC GGQSYTGSTT CASPYVCKVS NAYYSQCLPA


 


SEQ ID NO: 56
39971383
Magnaporthe grisea
MKRALCASLS LLAAAVAQQV GTNEPEVHPK MTWKKCSSGG SCSTVNGEVV IDGNWRWIHN IGGYENCYSG




70-15
NKWTSVCSTN ADCATKCAME GAKYQETYGV STSGDALTLK FVQQNSSGKN VGSRMYLMNG ANKYQMFTLK





NNEFAFDVDL SSVECGMNSA LYFVPMKEDG GMSTEPNNKA GAKYGTGYCD AQCARDLKFI GGKGNIEGWQ





PSSTDSSAGI GAQGACCAEI DIWESNKNAF AFTPHPCENN EYHVCTEPNC GGTYADDRYG GGCDANGCDY





NPYRMGNPDF YGPGKTIDTN RKFTVISRFE NNRNYQILMQ DGVAHRIPGP KFDGLEGETG ELNEQFCTDQ





FTVFDERNRF NEVGGWSKLN AAYEIPMVLV MSIWSDHFAN MLWLDSTYPP EKAGQPGSAR GPCPADGGDP





NGVVNQYPNA KVIWSNVRFG PIGSTYQVD


 


SEQ ID NO: 57
39973029
Magnaporthe grisea
MQLTKAGVFL GALMGGAAAQ QVGTQTAENH PKMTWKKCTG KASCTTVNGE VVIDANWRWL HDASSKNCYD




70-15
GNRWTDSCRT ASDCAAKCSL EGADYAKTYG ASTSGDALSL KFVTRHDYGT NIGSRFYLMN GASKYQMFSL





LGNEFAFDVD LSTIECGLNS ALYFVAMEED GGMKSYSSNK AGAKYGTGYC DAQCARDLKF VGGKANIEGW





KPSSNDANAG VGPYGACCAE IDVWESNAHA FAFTPHPCTD NKYHVCQDSN CGGTYSDDRF AGKCDANGCD





INPYRLGNTD FYGKGKTVDT SKKFTVVTRF ERDALTQFFV QNNKRIDMPS PALEGLPATG AITAEYCTNV





FNVFGDRNRF DEVGGWSQLQ QALSLPMVLV MSIWDDHYSN MLWLDSVYPP DKEGSPGAAR GDCPQDSGVP





SEVESQIPGA TVVWSNIRFG PVGSTVNV


 


SEQ ID NO: 58
1170141
Fusarium
MYRIVATASA LIAAARAQQV CSLNTETKPA LTWSKCTSSG CSDVKGSVVI DANWRWTHQT SGSTNCYTGN




oxysporum
KWDTSICTDG KTCAEKCCLD GADYSGTYGI TSSGNQLSLG FVTNGPYSKN IGSRTYLMEN ENTYQMFQLL





GNEFTFDVDV SGIGCGLNGA PHFVSMDEDG GKAKYSGNKA GAKYGTGYCD AQCPRDVKFI NGVANSEGWK





PSDSDVNAGV GNLGTCCPEM DIWEANSIST AFTPHPCTKL TQHSCTGDSC GGTYSSDRYG GTCDADGCDF





NAYRQGNKTF YGPGSNFNID TTKKMTVVTQ FHKGSNGRLS EITRLYVQNG KVIANSESKI AGNPGSSLTS





DFCSKQKSVF GDIDDFSKKG GWNGMSDALS APMVLVMSLW HDHHSNMLWL DSTYPTDSTK VGSQRGSCAT





TSGKPSDLER DVPNSKVSFS NIKFGPIGST YKSDGTTPNP PASSSTTGSS TPTNPPAGSV DQWGQCGGQN





YSGPTTCKSP FTCKKINDFY SQCQ


 


SEQ ID NO: 59
121710012
Aspergillus clavatus
MYQRALLFSA LATAVSAQQV GTQKAEVHPA LTWQKCTAAG SCTDQKGSVV IDANWRWLHS TEDTTNCYTG




NRRL 1
NEWNAELCPD NEACAKNCAL DGADYSGTYG VTADGSSLKL NFVTSANVGS RLYLMEDDET YQMFNLLNNE





FTFDVDVSNL PCGLNGALYF VSMDADGGLS KYPGNKAGAK YGTGYCDSQC PRDLKFINGE ANVEGWKPSD





NDKNAGVGGY GSCCPEMDIW EANSISTAYT PHPCDGMEQT RCDGNDCGGT YSSTRYAGTC DPDGCDFNSF





RMGNESFYGP GGLVDTKSPI TVVTQFVTAG GTDSGALKEI RRVYVQGGKV IGNSASNVAG VEGDSITSDF





CTAQKKAFGD EDIFSKHGGL EGMGKALNKM ALIVSIWDDH ASSMMWLDST YPVDADASTP GVARGTCEHG





LGDPETVESQ HPDASVTFSN IKFGPIGSTY KSV


 


SEQ ID NO: 60
17902580
Penicillium
MSALNSFNMY KSALILGSLL ATAGAQQIGT YTAETHPSLS WSTCKSGGSC TTNSGAITLD ANWRWVHGVN




funiculosum
TSTNCYTGNT WNTAICDTDA SCAQDCALDG ADYSGTYGIT TSGNSLRLNF VTGSNVGSRT YLMADNTHYQ





IFDLLNQEFT FTVDVSNLPC GLNGALYFVT MDADGGVSKY PNNKAGAQYG VGYCDSQCPR DLKFIAGQAN





VEGWTPSTNN SNTGIGNHGS CCAELDIWEA NSISEALTPH PCDTPGLTVC TADDCGGTYS SNRYAGTCDP





DGCDFNPYRL GVTDFYGSGK TVDTTKPFTV VTQFVTDDGT SSGSLSEIRR YYVQNGVVIP QPSSKISGIS





GNVINSDFCA AELSAFGETA SFTNHGGLKN MGSALEAGMV LVMSLWDDYS VNMLWLDSTY PANETGTPGA





ARGSCPTTSG NPKTVESQSG SSYVVFSDIK VGPFNSTFSG GTSTGGSTTT TASGTTSTKA STTSTSSTST





GTGVAAHWGQ CGGQGWTGPT TCASGTTCTV VNPYYSQCL


 


SEQ ID NO: 61
1346226
Humicola grisea var
MRTAKFATLA ALVASAAAQQ ACSLTTERHP SLSWNKCTAG GQCQTVQASI TLDSNWRWTH QVSGSTNCYT




thermoidea
GNKWDTSICT DAKSCAQNCC VDGADYTSTY GITTNGDSLS LKFVTKGQHS TNVGSRTYLM DGEDKYQTFE





LLGNEFTFDV DVSNIGCGLN GALYFVSMDA DGGLSRYPGN KAGAKYGTGY CDAQCPRDIK FINGEANIEG





WTGSTNDPNA GAGRYGTCCS EMDIWEANNM ATAFTPHPCT IIGQSRCEGD SCGGTYSNER YAGVCDPDGC





DFNSYRQGNK TFYGKGMTVD TTKKITVVTQ FLKDANGDLG EIKRFYVQDG KIIPNSESTI PGVEGNSITQ





DWCDRQKVAF GDIDDFNRKG GMKQMGKALA GPMVLVMSIW DDHASNMLWL DSTFPVDAAG KPGAERGACP





TTSGVPAEVE AEAPNSNVVF SNIRFGPIGS TVAGLPGAGN GGNNGGNPPP PTTTTSSAPA TTTTASAGPK





AGRWQQCGGI GFTGPTQCEE PYICTKLNDW YSQCL


 


SEQ ID NO: 62
156712282
Chaetomium
MMYKKFAALA ALVAGASAQQ ACSLTAENHP SLTWKRCTSG GSCSTVNGAV TIDANWRWTH TVSGSTNCYT




thermophilum
GNQWDTSLCT DGKSCAQTCC VDGADYSSTY GITTSGDSLN LKFVTKHQYG TNVGSRVYLM ENDTKYQMFE





LLGNEFTFDV DVSNLGCGLN GALYFVSMDA DGGMSKYSGN KAGAKYGTGY CDAQCPRDLK FINGEANVGN





WTPSTNDANA GFGRYGSCCS EMDVWEANNM ATAFTPHPCT TVGQSRCEAD TCGGTYSSDR YAGVCDPDGC





DFNAYRQGDK TFYGKGMTVD TNKKMTVVTQ FHKNSAGVLS EIKRFYVQDG KIIANAESKI PGNPGNSITQ





EYCDAQKVAF SNTDDFNRKG GMAQMSKALA GPMVLVMSVW DDHYANMLWL DSTYPIDQAG APGAERGACP





TTSGVPAEIE AQVPNSNVIF SNIRFGPIGS TVPGLDGSNP GNPTTTVVPP ASTSTSRPTS STSSPVSTPT





GQPGGCTTQK WGQCGGIGYT GCTNCVAGTT CTQLNPWYSQ CL


 


SEQ ID NO: 63
169768818
Aspergillus oryzae
MASLSLSKIC RNALILSSVL STAQGQQVGT YQTETHPSMT WQTCGNGGSC STNQGSVVLD ANWRWVHQTG




RIB40
SSSNCYTGNK WDTSYCSTND ACAQKCALDG ADYSNTYGIT TSGSEVRLNF VTSNSNGKNV GSRVYMMADD





THYEVYKLLN QEFTFDVDVS KLPCGLNGAL YFVVMDADGG VSKYPNNKAG AKYGTGYCDS QCPRDLKFIQ





GQANVEGWVS STNNANTGTG NHGSCCAELD IWESNSISQA LTPHPCDTPT NTLCTGDACG GTYSSDRYSG





TCDPDGCDFN PYRVGNTTFY GPGKTIDTNK PITVVTQFIT DDGTSSGTLS EIKRFYVQDG VTYPQPSADV





SGLSGNTINS EYCTAENTLF EGSGSFAKHG GLAGMGEAMS TGMVLVMSLW DDYYANMLWL DSNYPTNEST





SKPGVARGTC STSSGVPSEV EASNPSAYVA YSNIKVGPIG STFKS


 


SEQ ID NO: 64
46241270
Gibberella pulicaris
MYRAIATASA LIAAVRAQQV CSLTPETKPA LSWSKCTSSG CSNVQGSVTI DANWRWTHQL SGSTNCYTGN





KWDTSICTSG KVCAEKCCID GAEYASTYGI TSSGNQLSLS FVTKGAYGTN IGSRTYLMED ENTYQMFQLL





GNEFTFDVDV SNIGCGLNGA LYFVSMDADG GKAKYPGNKA GAKYGTGYCD AQCPRDVKFI NGQANSDGWQ





PSKSDVNAGI GNMGTCCPEM DIWEANSIST AYTPHPCTKL TQHSCTGDSC GGTYSNDRYG GTCDADGCDF





NAYRQGNKTF YGPGSGFNVD TTKKVTVVTQ FHKGSNGRLS EITRLYVQNG KVIANSESKI AGVPGSSLTP





EFCTAQKKVF GDTDDFAKKG AWSGMSDALE APMVLVMSLW HDHHSNMLWL DSTYPTDSTK LGAQRGSCST





SSGVPADLEK NVPNSKVAFS NIKFGPIGST YKEGVPEPTN PTNPTNPTNP TNPGTVDQWA QCGGTNYSGP





TACKSPFTCK KINDFYSQCQ


 


SEQ ID NO: 65
49333363
Volvariella volvacea
MFPKSSLLVL SFLATAYAQQ VGTQTAEVHP SLNWARCTSS GCTNVAGSVT LDANWRWLHT TSGYTNCYTG





NSWNTTLCPD GATCAQNCAL DGANYQSTCG ITTSGNALTL KFVTQGEQKN IGSRVYLMAS ESRYEMFGLL





NKEFTFDVDV SNLPCGLNGA LYFSSMDADG GMAKNPGNKA GAKYGTGYCD SQCPRDIKFI NGEANVAGWN





GSPNDTNAGT GNWGACCNEM DIWEANSISA AYTPHPCTVQ GLSRCSGTAC GTNDRYGTVC DPDGCDFNSY





RMGDKTYYGP GGTGVDTRSK FTVVTQFLTN NNSSSGTLSE IRRLYVQNGR VVQNSKVNIP GMSNTLDSIT





TGFCDSQKTA FGDTRSFQNK GGMSAMGQAL GAGMVLVLSV WDDHAANMLW LDSNYPVDAD PSKPGIARGT





CSTTSGKPTD VEQSAANSSV TFSNIKFGDI GTTYTGGSVT TTPGNPGTTT STAPGAVQTK WGQCGGQGWT





GPTRCESGST CTVVNQWYSQ CI


 


SEQ ID NO: 66
46395332
Irpex lacteus
MFRKAALLAF SFLAIAHGQQ VGTNQAENHP SLPSQHCTAS GCTTSSTSVV LDANWRWVHT TTGYTNCYTG





QTWDASICPD GVTCAKACAL DGADYSGTYG ITTSGNALTL QFVKGTNVGS RVYLLQDASN YQLFKLINQE





FTFDVDMSNL PCGLNGAVYL SQMDQDGGVS RFPTNTAGAK YGTGYCDSQC PRDIKFINGE ANVAGWTGSS





SDPNSGTGNY GTCCSEMDIW EANSVAAAYT PHPCSVNQQT RCTGADCGQD ANRYKGVCDP DGCDFNSFRM





GDQTFLGKGL TVDTSRKFTI VTQFISDDGT SSGNLAEIRR FYVQDGKVIP NSKVNIAGCD AVNSITDKFC





TQQKTAFGDT NRFADQGGLK QMGAALKSGM VLALSLWDDH AANMLWLDSD YPTTADASKP GVARGTCPNT





SGVPKDVESQ SGSATVTYSN IKWGDLNSTF SGTASNPTGP SSSPSGPSSS SSSTAGSQPT QPSSGSVAQW





GQCGGIGYSG ATGCVSPYTC HVVNPYYSQC Y


 


SEQ ID NO: 67
50844407 #
Chaetomium
TETHPRLTWK RCTSGGNCST VNGAVTIDAN WRWTHTVSGS TNCYTGNEWD TSICSDGKSC AQTCCVDGAD




thermophilum var
YSSTYGITTS GDSLNLKFVT KHQHGTNVGS RVYLMENDTK YQMFELLGNE FTFDVDVSNL GCGLNGALYF




thermophilum
VSMDADGGMS KYSGNKAGAK YGTGYCDAQC PRDLKFINGE ANIENWTPST NDANAGFGRY GSCCSEMDIW





EANNMATAFT PHPCTIIGQS RCEGNSCGGT YSSERYAGVC DPDGCDFNAY RQGDKTFYGK GMTVDTTKKM





TVVTQFHKNS AGVLSEIKRF YVQDGKIIAN AESKIPGNPG NSITQEWCDA QKVAFGDIDD FNRKGGMAQM





SKALEGPMVL VMSVWDDHYA NMLWLDSTYP IDKAGTPGAE RGACPTTSGV PAEIEAQVPN SNVIFSNIRF





GPIGSTVPGL DGSTPSNPTA TVAPPTSTTT SVRSSTTQIS TPTSQPGGCT TQKWGQCGGI GYTGCTNCVA





GTTCTELNPW YSQCL


 


SEQ ID NO: 68
4586347
Irpex lacteus
MFHKAVLVAF SLVTIVHGQQ AGTQTAENHP QLSSQKCTAG GSCTSASTSV VLDSNWRWVH TTSGYTNCYT





GNTWDASICS DPVSCAQNCA LDGADYAGTY GITTSGDALT LKFVTGSNVG SRVYLMEDET NYQMFKLMNQ





EFTFDVDVSN LPCGLNGAVY FVQMDQDGGT SKFPNNKAGA KFGTGYCDSQ CPQDIKFING EANIVDWTAS





AGDANSGTGS FGTCCQEMDI WEANSISAAY TPHPCTVTEQ TRCSGSDCGQ GSDRFNGICD PDGCDFNSFR





MGNTEFYGKG LTVDTSQKFT IVTQFISDDG TADGNLAEIR RFYVQNGKVI PNSVVQITGI DPVNSITEDF





CTQQKTVFGD TNNFAAKGGL KQMGEAVKNG MVLALSLWDD YAAQMLWLDS DYPTTADPSQ PGVARGTCPT





TSGVPSQVEG QEGSSSVIYS NIKFGDLNST FTGTLTNPSS PAGPPVTSSP SEPSQSTQPS QPAQPTQPAG





TAAQWAQCGG MGFTGPTVCA SPFTCHVLNP YYSQCY


 


SEQ ID NO: 69
3980202
Phanerochaete
MFRAAALLAF TCLAMVSGQQ AGTNTAENHP QLQSQQCTTS GGCKPLSTKV VLDSNWRWVH STSGYTNCYT




chrysosporium
GNEWNTSLCP DGKTCAANCA LDGADYSGTY GITSTGTALT LKFVTGSNVG SRVYLMADDT HYQLLKLLNQ





EFTFDVDMSN LPCGLNGALY LSAMDADGGM SKYPGNKAGA KYGTGYCDSQ CPKDIKFING EANVGNWTET





GSNTGTGSYG TCCSEMDIWE ANNDAAAFTP HPCTTTGQTR CSGDDCARNT GLCDHGDGCD FNSFRMGDKT





FLGKGMTVDT SKPFTDVTQF LTNDNTSTGT LSEIRRIYIQ NGKVIQNSVA NIPGVDPVNS ITDNFCAQQK





TAFGDTNWFA QKGGLKQMGE ALGNGMVLAL SIWDDHAANM LWLDSDYPTD KDPSAPGVAR GTCATTSGVP





SDVESQVPNS QVVFSNIKFG DIGSTFSGTS SPNPPGGSTT SSPVTTSPTP PPTGPTVPQW GQCGGIGYSG





STTCASPYTC HVLNPYYSQC Y


 


SEQ ID NO: 70
27125837
Melanocarpus
MMMKQYLQYL AAALPLVGLA AGQRAGNETP ENHPPLTWQR CTAPGNCQTV NAEVVIDANW RWLHDDNMQN




albomyces
CYDGNQWTNA CSTATDCAEK CMIEGAGDYL GTYGASTSGD ALTLKFVTKH EYGTNVGSRF YLMNGPDKYQ





MFNLMGNELA FDVDLSTVEC GINSALYFVA MEEDGGMASY PSNQAGARYG TGYCDAQCAR DLKFVGGKAN





IEGWKSSTSD PNAGVGPYGS CCAEIDVWES NAYAFAFTPH ACTTNEYHVC ETTNCGGTYS EDRFAGKCDA





NGCDYNPYRM GNPDFYGKGK TLDTSRKFTV VSRFEENKLS QYFIQDGRKI EIPPPTWEGM PNSSEITPEL





CSTMFDVFND RNRFEEVGGF EQLNNALRVP MVLVMSIWDD HYANMLWLDS IYPPEKEGQP GAARGDCPTD





SGVPAEVEAQ FPDAQVVWSN IRFGPIGSTY DF


 


SEQ ID NO: 71
171696102
Podospora anserina
MYRSATFLTF ASLVLGQQVG TYTAERHPSM PIQVCTAPGQ CTRESTEVVL DANWRWTHIT NGYTNCYTGN





EWNATACPDG ATCAKNCAVD GADYSGTYGI TTPSSGALRL QFVKKNDNGQ NVGSRVYLMA SSDKYKLFNL





LNKEFTFDVD VSKLPCGLNG AVYFSEMLED GGLKSFSGNK AGAKYGTGYC DSQCPQDIKF INGEANVEGW





GGADGNSGTG KYGICCAEMD IWEANSDATA YTPHVCSVNE QTRCEGVDCG AGSDRYNSIC DKDGCDFNSY





RLGNREFYGP GKTVDTTRPF TIVTQFVTDD GTDSGNLKSI HRYYVQDGNV IPNSVTEVAG VDQTNFISEG





FCEQQKSAFG DNNYFGQLGG MRAMGESLKK MVLVLSIWDD HAVNMNWLDS IFPNDADPEQ PGVARGRCDP





ADGVPATIEA AHPDAYVIYS NIKFGAINST FTAN


 


SEQ ID NO: 72
3913802
Cochliobolus
MYRTLAFASL SLYGAARAQQ VGTSTAENHP KLTWQTCTGT GGTNCSNKSG SVVLDSNWRW AHNVGGYTNC




carbonum
YTGNSWSTQY CPDGDSCTKN CAIDGADYSG TYGITTSNNA LSLKFVTKGS FSSNIGSRTY LMETDTKYQM





FNLINKEFTF DVDVSKLPCG LNGALYFVEM AADGGIGKGN NKAGAKYGTG YCDSQCPHDI KFINGKANVE





GWNPSDADPN GGAGKIGACC PEMDIWEANS ISTAYTPHPC RGVGLQECSD AASCGDGSNR YDGQCDKDGC





DFNSYRMGVK DFYGPGATLD TTKKMTVITQ FLGSGSSLSE IKRFYVQNGK VYKNSQSAVA GVTGNSITES





FCTAQKKAFG DTSSFAALGG LNEMGASLAR GHVLIMSLWG DHAVNMLWLD STYPTDADPS KPGAARGTCP





TTSGKPEDVE KNSPDATVVF SNIKFGPIGS TFAQPA


 


SEQ ID NO: 73
50403723
Trichoderma viride
MYQKLALISA FLATARAQSA CTLQAETHPP LTWQKCSSGG TCTQQTGSVV IDANWRWTHA TNSSTNCYDG





NTWSSTLCPD NETCAKNCCL DGAAYASTYG VTTSADSLSI GFVTQSAQKN VGARLYLMAS DTTYQEFTLL





GNEFSFDVDV SQLPCGLNGA LYFVSMDADG GVSKYPTNTA GAKYGTGYCD SQCPRDLKFI NGQANVEGWE





PSSNNANTGI GGHGSCCSEM DIWEANSISE ALTPHPCTTV GQEICDGDSC GGTYSGDRYG GTCDPDGCDW





NPYRLGNTSF YGPGSSFTLD TTKKLTVVTQ FETSGAINRY YVQNGVTFQQ PNAELGDYSG NSLDDDYCAA





EEAEFGGSSF SDKGGLTQFK KATSGGMVLV MSLWDDYYAN MLWLDSTYPT NETSSTPGAV RGSCSTSSGV





PAQLESNSPN AKVVYSNIKF GPIGSTGNSS GGNPPGGNPP GTTTTRRPAT STGSSPGPTQ THYGQCGGIG





YSGPTVCASG STCQVLNPYY SQCL


 


SEQ ID NO: 74
3913798
Aspergillus
MVDSFSIYKT ALLLSMLATS NAQQVGTYTA ETHPSLTWQT CSGSGSCTTT SGSVVIDANW RWVHEVGGYT




aculeatus
NCYSGNTWDS SICSTDTTCA SECALEGATY ESTYGVTTSG SSLRLNFVTT ASQKNIGSRL YLLADDSTYE





TFKLFNREFT FDVDVSNLPC GLNGALYFVS MDADGGVSRF PTNKAGAKYG TGYCDSQCPR DLKFIDGQAN





IEGWEPSSTD VNAGTGNHGS CCPEMDIWEA NSISSAFTAH PCDSVQQTMC TGDTCGGTYS DTTDRYSGTC





DPDGCDFNPY RFGNTNFYGP GKTVDNSKPF TVVTQFITHD GTDTGTLTEI RRLYVQNGVV IGNGPSTYTA





ASGNSITESF CKAEKTLFGD TNVFETHGGL SAMGDALGDG MVLVLSLWDD HAADMLWLDS DYPTTSCASS





PGVARGTCPT TTGNATYVEA NYPNSYVTYS NIKFGTLNST YSGTSSGGSS SSSTTLTTKA STSTTSSKTT





TTTSKTSTTS SSSTNVAQLY GQCGGQGWTG PTTCASGTCTKQNDYYSQCL


 


SEQ ID NO: 75
66828465
Dictyostelium
MYRILKSFIL LSLVNMSLSQ KIGKLTPEVH PPMTFQKCSE GGSCETIQGE VVVDANWRWV HSAQGQNCYT




discoideum
GNTWNPTICP DDETCAENCY LDGANYESVY GVTTSEDSVR LNFVTQSQGK NIGSRLFLMS NESNYQLFHV





LGQEFTFDVD VSNLDCGLNG ALYLVSMDSD GGSARFPTNE AGAKYGTGYC DAQCPRDLKF ISGSANVDGW





IPSTNNPNTG YGNLGSCCAE MDLWEANNMA TAVTPHPCDT SSQSVCKSDS CGGAASSNRY GGICDPDGCD





YNPYRMGNTS FFGPNKMIDT NSVITVVTQF ITDDGSSDGK LTSIKRLYVQ DGNVISQSVS TIDGVEGNEV





NEEFCTNQKK VFGDEDSFTK HGGLAKMGEA LKDGMVLVLS LWDDYQANML WLDSSYPTTS SPTDPGVARG





SCPTTSGVPS KVEQNYPNAY VVYSNIKVGP IDSTYKK


 


SEQ ID NO: 76
156060391
Sclerotinia
MISRVLAISS LLAAARAQQI GTNTAEVHPA LTSIVIDANW RWLHTTSGYT NCYTGNSWDA TLCPDAVTCA




sclerotiorum 1980
ANCALDGADY SGTYGITTSG NSLKLNFVTK GANTNVGSRT YLMAAGSKTQ YQLLKLLGQE FTFDVDVSNL





PCGLNGALYF AEMDADGGVS RFPTNKAGAQ YGTGYCDAQC PQDIKFINGQ ANSVGWTPSS NDVNTGTGQY





GSCCSEMDIW EANKISAAYT PHPCSVDGQT RCTGTDCGIG ARYSSLCDAD GCDFNSYRMG DTGFYGAGLT





VDTSKVFTVV TQFITNDGTT SGTLSEIRRF YVQNGKVIPN SQSKVTGVSG NSITDSFCAA QKTAFGDTNE





FATKGGLATM SKALAKGMVL VMSIWDDHSA NMLWLDAPYP ASKSPSAAGV SRGSCSASSG VPADVEANSP





GASVTYSNIK WGPINSTYSA GTGSNTGSGS GSTTTLVSSV PSSTPTSTTG VPKYGQCGGS GYTGPTNCIG





STCVSMGQYY SQCQ


 


SEQ ID NO: 77
116181754
Chaetomium
MYRQVATALS FASLVLGQQV GTLTAETHPS LPIEVCTAPG SCTKEDTTVV LDANWRWTHV TDGYTNCYTG




globosum CBS 148-
NAWNETACPD GKTCAANCAI DGAEYEKTYG ITTPEEGALR LNFVTESNVG SRVYLMAGED KYRLFNLLNK




51
EFTMDVDVSN LPCGLNGAVY FSEMDEDGGM SRFEGNKAGA KYGTGYCDSQ CPRDIKFING EANSEGWGGE





DGNSGTGKYG TCCAEMDIWE ANLDATAYTP HPCKVTEQTR CEDDTECGAG DARYEGLCDR DGCDFNSFRL





GNKEFYGPEK TVDTSKPFTL VTQFVTADGT DTGALQSIRR FYVQDGTVIP NSETVVEGVD PTNEITDDFC





AQQKTAFGDN NHFKTIGGLP AMGKSLEKMV LVLSIWDDHA VYMNWLDSNY PTDADPTKPG VARGRCDPEA





GVPETVEAAH PDAYVIYSNI KIGALNSTFA AA


 


SEQ ID NO: 78
145230535
Aspergillus niger
MSSFQVYRAA LLLSILATAN AQQVGTYTTE THPSLTWQTC TSDGSCTTND GEVVIDANWR WVHSTSSATN





CYTGNEWDTS ICTDDVTCAA NCALDGATYE ATYGVTTSGS ELRLNFVTQG SSKNIGSRLY LMSDDSNYEL





FKLLGQEFTF DVDVSNLPCG LNGALYFVAM DADGGTSEYS GNKAGAKYGT GYCDSQCPRD LKFINGEANC





DGWEPSSNNV NTGVGDHGSC CAEMDVWEAN SISNAFTAHP CDSVSQTMCD GDSCGGTYSA SGDRYSGTCD





PDGCDYNPYR LGNTDFYGPG LTVDTNSPFT VVTQFITDDG TSSGTLTEIK RLYVQNGEVI ANGASTYSSV





NGSSITSAFC ESEKTLFGDE NVFDKHGGLE GMGEAMAKGM VLVLSLWDDY AADMLWLDSD YPVNSSASTP





GVARGTCSTD SGVPATVEAE SPNAYVTYSN IKFGPIGSTY SSGSSSGSGS SSSSSSTTTK ATSTTLKTTS





TTSSGSSSTS AAQAYGQCGG QGWTGPTTCV SGYTCTYENA YYSQCL


 


SEQ ID NO: 79
46241266
Nectria
MYRAIATASA LLATARAQQV CTLNTENKPA LTWAKCTSSG CSNVRGSVVV DANWRWAHST SSSTNCYTGN




haematococca mpVI
TWDKTLCPDG KTCADKCCLD GADYSGTYGV TSSGNQLNLK FVTVGPYSTN VGSRLYLMED ENNYQMFDLL





GNEFTFDVDV NNIGCGLNGA LYFVSMDKDG GKSRFSTNKA GAKYGTGYCD AQCPRDVKFI NGVANSDEWK





PSDSDKNAGV GKYGTCCPEM DIWEANKIST AYTPHPCKSL TQQSCEGDAC GGTYSATRYA GTCDPDGCDF





NPYRQGNKTF YGPGSGFNVD TTKKVTVVTQ FIKGSDGKLS EIKRLYVQNG KVIGNPQSEI ANNPGSSVTD





SFCKAQKVAF NDPDDFNKKG GWSGMSDALA KPMVLVMSLW HDHYANMLWL DSTYPKGSKT PGSARGSCPE





DSGDPDTLEK EVPNSGVSFS NIKFGPIGST YTGTGGSNPD PEEPEEPEEP VGTVPQYGQC GGINYSGPTA





CVSPYKCNKI NDFYSQCQ


 


SEQ ID NO: 80
1q9h (PDB) #
Talaromyces
EQAGTATAEN HPPLTWQECT APGSCTTQNG AVVLDANWRW VHDVNGYTNC YTGNTWDPTY CPDDETCAQN




emersonii
CALDGADYEG TYGVTSSGSS LKLNFVTGSN VGSRLYLLQD DSTYQIFKLL NREFSFDVDV SNLPCGLNGA





LYFVAMDADG GVSKYPNNKA GAKYGTGYCD SQCPRDLKFI DGEANVEGWQ PSSNNANTGI GDHGSCCAEM





DVWEANSISN AVTPHPCDTP GQTMCSGDDC GGTYSNDRYA GTCDPDGCDF NPYRMGNTSF YGPGKIIDTT





KPFTVVTQFL TDDGTDTGTL SEIKRFYIQN SNVIPQPNSD ISGVTGNSIT TEFCTAQKQA FGDTDDFSQH





GGLAKMGAAM QQGMVLVMSL WDDYAAQMLW LDSDYPTDAD PTTPGIARGT CPTDSGVPSD VESQSPNSYV





TYSNIKFGPI NSTFTAS


 


SEQ ID NO: 81
157362170
Polyporus
MFPTLALVSL SFLAIAYGQQ VGTLTAETHP KLSVSQCTAG GSCTTVQRSV VLDSNWRWLH DVGGSTNCYT




arcularius
GNTWDDSLCP DPTTCAANCA LDGADYSGTY GITTSGNALS LKFVTQGPYS TNIGSRVYLL SEDDSTYEMF





NLKNQEFTFD VDMSALPCGL NGALYFVEMD KDGGSGRFPT NKAGSKYGTG YCDTQCPHDI KFINGEANVL





DWAGSSNDPN AGTGHYGTCC NEMDIWEANS MGAAVTPHVC TVQGQTRCEG TDCGDGDERY DGICDKDGCD





FNSWRMGDQT FLGPGKTVDT SSKFTVVTQF ITADNTTSGD LSEIRRLYVQ NGKVIANSKT QIAGMDAYDS





ITDDFCNAQK TTFGDTNTFE QMGGLATMGD AFETGMVLVM SIWDDHEAKM LWLDSDYPTD ADASAPGVSR





GPCPTTSGDP TDVESQSPGA TVIFSNIKTG PIGSTFTS


 


SEQ ID NO: 82
7804885
Leptosphaeria
MLSASKAAAI LAFCAHTASA WVVGDQQTET HPKLNWQRCT GKGRSSCTNV NGEVVIDANW RWLAHRSGYT




maculans
NCYTGSEWNQ SACPNNEACT KNCAIEGSDY AGTYGITTSG NQMNIKFITK RPYSTNIGAR TYLMKDEQNY





EMFQLIGNEF TFDVDLSQRC GMNGALYFVS MPQKGQGAPG AKYGTGYCDA QCARDLKFVR GSANAEGWTK





SASDPNSGVG KKGACCAQMD VWEANSAATA LTPHSCQPAG YSVCEDTNCG GTYSEDRYAG TCDANGCDFN





PFRVGVKDFY GKGKTVDTTK KMTVVTQFVG SGNQLSEIKR FYVQDGKVIA NPEPTIPGME WCNTQKKVFQ





EEAYPFNEFG GMASMSEGMS QGMVLVMSLW DDHYANMLWL DSNWPREADP AKPGVARRDC PTSGGKPSEV





EAANPNAQVM FSNIKFGPIG STFAHAA


 


SEQ ID NO: 83
121852
Phanerochaete
MFRTATLLAF TMAAMVFGQQ VGTNTARSHP ALTSQKCTKS GGCSNLNTKI VLDANWRWLH STSGYTNCYT




chrysosporium
GNQWDATLCP DGKTCAANCA LDGADYTGTY GITASGSSLK LQFVTGSNVG SRVYLMADDT HYQMFQLLNQ





EFTFDVDMSN LPCGLNGALY LSAMDADGGM AKYPTNKAGA KYGTGYCDSQ CPRDIKFING EANVEGWNAT





SANAGTGNYG TCCTEMDIWE ANNDAAAYTP HPCTTNAQTR CSGSDCTRDT GLCDADGCDF NSFRMGDQTF





LGKGLTVDTS KPFTVVTQFI TNDGTSAGTL TEIRRLYVQN GKVIQNSSVK IPGIDPVNSI TDNFCSQQKT





AFGDTNYFAQ HGGLKQVGEA LRTGMVLALS IWDDYAANML WLDSNYPTNK DPSTPGVARG TCATTSGVPA





QIEAQSPNAY VVFSNIKFGD LNTTYTGTVS SSSVSSSHSS TSTSSSHSSS STPPTQPTGV TVPQWGQCGG





IGYTGSTTCA SPYTCHVLNP YYSQCY


 


SEQ ID NO: 84
126013214
Penicillium
MYQRALLFSA LMAGVSAQQV GTQKPETHPP LAWKECTSSG CTSKDGSVVI DANWRWVHSV DGYKNCYTGN




decumbens
EWDSTLCPDD ATCATNCAVD GADYAGTYGA TTEGDSLSIN FVTGSNIGSR FYLMEDENKY QMFKLLNKEF





TFDVDVSTLP CGLNGALYFV SMDADGGMSK YETNKAGAKY GTGYCDSQCP RDLKFINGKG NVEGWKPSAN





DKNAGVGPHG SCCAEMDIWE ANSISTALTP HPCDTNGQTI CEGDSCGGTY STTRYAGTCD PDGCDFNPFR





MGNESFYGPG KMVDTKSKMT VVTQFITSDG TDTGSLKEIK RVYVQNGKVI ANSASDVSGI TGNSITSDFC





TAQKKTFGDE DVFNKHGGLS GMGDALGEGM VLVMSLWDDH NSNMLWLDGE KYPTDAAASK AGVSRGTCST





DSGKPSTVES ESGSAKVVFS NIKVGSIGST FSA


 


SEQ ID NO: 85
156048578
Sclerotinia
MTSKIALASL FAAAYGQQIG TYTTETHPSL TWQSCTAKGS CTTQSGSIVL DGNWRWTHST TSSTNCYTGN




sclerotiorum 1980
TWDATLCPDD ATCAQNCALD GADYSGTYGI TTSGDSLRLN FVTQTANKNV GSRVYLLADN THYKTFNLLN





QEFTFDVDVS NLPCGLNGAV YFANLPADGG ISSTNKAGAQ YGTGYCDSQC PRDGKFINGK ANVDGWVPSS





NNPNTGVGNY GSCCAEMDIW EANSISTAVT PHSCDTVTQT VCTGDNCGGT YSTTRYAGTC DPDGCDFNPY





RQGNESFYGP GKTVDTNSVF TIVTQFLTTD GTSSGTLNEI KRFYVQNGKV IPNSESTISG VTGNSITTPF





CTAQKTAFGD PTSFSDHGGL ASMSAAFEAG MVLVLSLWDD YYANMLWLDS TYPTTKTGAG GPRGTCSTSS





GVPASVEASS PNAYVVYSNI KVGAINSTFG


 


SEQ ID NO: 86
156712278
Acremonium
MYTKFAALAA LVATVRGQAA CSLTAETHPS LQWQKCTAPG SCTTVSGQVT IDANWRWLHQ TNSSTNCYTG




thermophilum
NEWDTSICSS DTDCATKCCL DGADYTGTYG VTASGNSLNL KFVTQGPYSK NIGSRMYLME SESKYQGFTL





LGQEFTFDVD VSNLGCGLNG ALYFVSMDLD GGVSKYTTNK AGAKYGTGYC DSQCPRDLKF INGQANIDGW





QPSSNDANAG LGNHGSCCSE MDIWEANKVS AAYTPHPCTT IGQTMCTGDD CGGTYSSDRY AGICDPDGCD





FNSYRMGDTS FYGPGKTVDT GSKFTVVTQF LTGSDGNLSE IKRFYVQNGK VIPNSESKIA GVSGNSITTD





FCTAQKTAFG DTNVFEERGG LAQMGKALAE PMVLVLSVWD DHAVNMLWLD STYPTDSTKP GAARGDCPIT





SGVPADVESQ APNSNVIYSN IRFGPINSTY TGTPSGGNPP GGGTTTTTTT TTSKPSGPTT TTNPSGPQQT





HWGQCGGQGW TGPTVCQSPY TCKYSNDWYS QCL


 


SEQ ID NO: 87
21449327
Aspergillus nidulans
MYQRALLFSA LLSVSRAQQA GTAQEEVHPS LTWQRCEASG SCTEVAGSVV LDSNWRWTHS VDGYTNCYTG




(also known as
NEWDATLCPD NESCAQNCAV DGADYEATYG ITSNGDSLTL KFVTGSNVGS RVYLMEDDET YQMFDLLNNE




Emericella nidulans)
FTFDVDVSNF PCGLNGALYF TSMDADGGLS KYEGNTAGAK YGTGYCDSQC PRDIKFINGL GNVEGWEPSD





SDANAGVGGM GTCCPEMDIW EANSISTAYT PHPCDSVEQT MCEGDSCGGT YSDDRYGGTC DPDGCDFNSY





RMGNTRFYGP GAIIDTSSKF TVVTQFIADG GSLSEIKRFY VQNGEVIPNS ESNISGVEGN SITSEFCTAQ





KTAFGDEDIF AQHGGLSAMG DAASAMVLIL SIWDDHHSSM MWLDSSYPTD ADPSQPGVAR GTCEQGAGDP





DVVESEHADA SVTFSNIKFG PIGSTF


 


SEQ ID NO: 88
171683762
Podospora anserine
MMMKQYLQYL AAGSLMTGLV AGQGVGTQQT ETHPRITWKR CTGKANCTTV QAEVVIDSNW RWIHTSGGTN




(S mat+)
CYDGNAWNTA ACSTATDCAS KCLMEGAGNY QQTYGASTSG DSLTLKFVTK HEYGTNVGSR FYLMNGASKY





QMFTLMNNEF TFDVDLSTVE CGLNSALYFV AMEEDGGMRS YPTNKAGAKY GTGYCDAQCA RDLKFVGGKA





NIEGWRESSN DENAGVGPYG GCCAEIDVWE SNAHAYAFTP HACENNNYHV CERDTCGGTY SEDRFAGGCD





ANGCDYNPYR MGNPDFYGKG KTVDTTKKFT VVTRFQDDNL EQFFVQNGQK ILAPAPTFDG IPASPNLTPE





FCSTQFDVFT DRNRFREVGD FPQLNAALRI PMVLVMSIWA DHYANMLWLD SVYPPEKEGE PGAARGPCAQ





DSGVPSEVKA NYPNAKVVWS NIRFGPIGST VNV


 


SEQ ID NO: 89
56718412
Thermoascus
MYQRALLFSF FLAAARAQQA GTVTAENHPS LTWQQCSSGG SCTTQNGKVV IDANWRWVHT TSGYTNCYTG




aurantiacus var
NTWDTSICPD DVTCAQNCAL DGADYSGTYG VTTSGNALRL NFVTQSSGKN IGSRLYLLQD DTTYQIFKLL




levisporus
GQEFTFDVDV SNLPCGLNGA LYFVAMDADG GLSKYPGNKA GAKYGTGYCD SQCPRDLKFI NGQANVEGWQ





PSANDPNAGV GNHGSCCAEM DVWEANSIST AVTPHPCDTP GQTMCQGDDC GGTYSSTRYA GTCDPDGCDF





NPYRQGNHSF YGPGKIVDTS SKFTVVTQFI TDDGTPSGTL TEIKRFYVQN GKVIPQSEST ISGVTGNSIT





TEYCTAQKAA FGDNTGFFTH GGLQKISQAL AQGMVLVMSL WDDHAANMLW LDSTYPTDAD PDTPGVARGT





CPTTSGVPAD VESQNPNSYV IYSNIKVGPI NSTFTAN


 


SEQ ID NO: 90
15824273
Pseudotrichonympha
MFAIVLLGLT RSLGTGTNQA ENHPSLSWQN CRSGGSCTQT SGSVVLDSNW RWTHDSSLTN CYDGNEWSSS




grassii
LCPDPKTCSD NCLIDGADYS GTYGITSSGN SLKLVFVTNG PYSTNIGSRV YLLKDESHYQ IFDLKNKEFT





FTVDDSNLDC GLNGALYFVS MDEDGGTSRF SSNKAGAKYG TGYCDAQCPH DIKFINGEAN VENWKPQTND





ENAGNGRYGA CCTEMDIWEA NKYATAYTPH ICTVNGEYRC DGSECGDTDS GNRYGGVCDK DGCDFNSYRM





GNTSFWGPGL IIDTGKPVTV VTQFVTKDGT DNGQLSEIRR KYVQGGKVIE NTVVNIAGMS SGNSITDDFC





NEQKSAFGDT NDFEKKGGLS GLGKAFDYGM VLVLSLWDDH QVNMLWLDSI YPTDQPASQP GVKRGPCATS





SGAPSDVESQ HPDSSVTFSD IRFGPIDSTY


 


SEQ ID NO: 91
115390801
Aspergillus terreus
MHQRALLFSA LVGAVRAQQA GTLTEEVHPP LTWQKCTADG SCTEQSGSVV IDSNWRWLHS TNGSTNCYTG




NIH2624
NTWDESLCPD NEACAANCAL DGADYESTYG ITTSGDALTL TFVTGENVGS RVYLMAEDDE SYQTFDLVGN





EFTFDVDVSN LPCGLNGALY FTSMDADGGV SKYPANKAGA KYGTGYCDSQ CPRDLKFING MANVEGWTPS





DNDKNAGVGG HGSCCPELDI WEANSISSAF TPHPCDDLGQ TMCSGDDCGG TYSETRYAGT CDPDGCDFNA





YRMGNTSYYG PDKIVDTNSV MTVVTQFIGD GGSLSEIKRL YVQNGKVIAN AQSNVDGVTG NSITSDFCTA





QKTAFGDQDI FSKHGGLSGM GDAMSAMVLI LSIWDDHNSS MMWLDSTYPE DADASEPGVA RGTCEHGVGD





PETVESQHPG ATVTFSKIKF GPIGSTYSSN STA


 


SEQ ID NO: 92
453223
Phanerochaete
MFRAAALLAF TCLAMVSGQQ AGTNTAENHP QLQSQQCTTS GGCKPLSTKV VLDSNWRWVH STSGYTNCYT




chrysosporium
GNEWDTSLCP DGKTCAANCA LDGADYSGTY GITSTGTALT LKFVTGSNVG SRVYLMADDT HYQLLKLLNQ





EFTFDVDMSN LPCGLNGALY LSAMDADGGM SKYPGNKAGA KYGTGYCDSQ CPKDIKFING EANVGNWTET





GSNTGTGSYG TCCSEMDIWE ANNDAAAFTP HPCTTTGQTR CSGDDCARNT GLCDGDGCDF NSFRMGDKTF





LGKGMTVDTS KPFTVVTQFL TNDNTSTGTL SEIRRIYIQN GKVIQNSVAN IPGVDPVNSI TDNFCAQQKT





AFGDTNWFAQ KGGLKQMGEA LGNGMVLALS IWDDHAANML WLDSDYPTDK DPSAPGVARG TCATTSGVPS





DVESQVPNSQ VVFSNIKFGD IGSTFSGTSS PNPPGGSTTS SPVTTSPTPP PTGPTVPQWG QCGGIGYSGS





TTCASPYTCH VLNPCESILS LQRSSNADQY LQTTRSATKR RLDTALQPRK


 


SEQ ID NO: 93
3132
Phanerochaete
MRTALALILA LAAFSAVSAQ QAGTITAETH PTLTIQQCTQ SGGCAPLTTK VVLDVNWRWI HSTTGYTNCY




chrysosporium
SGNTWDAILC PDPVTCAANC ALDGADYTGT FGILPSGTSV TLRPVDGLGL RLFLLADDSH YQMFQLLNKE





FTFDVEMPNM RCGSSGAIHL TAMDADGGLA KYPGNQAGAK YGTGFCSAQC PKGVKFINGQ ANVEGWLGTT





ATTGTGFFGS CCTDIALWEA NDNSASFAPH PCTTNSQTRC SGSDCTADSG LCDADGCNFN SFRMGNTTFF





GAGMSVDTTK LFTVVTQFIT SDNTSMGALV EIHRLYIQNG QVIQNSVVNI PGINPATSIT DDLCAQENAA





FGGTSSFAQH GGLAQVGEAL RSGMVLALSI VNSAADTLWL DSNYPADADP SAPGVARGTC PQDSASIPEA





PTPSVVFSNI KLGDIGTTFG AGSALFSGRS PPGPVPGSAP ASSATATAPP FGSQCGGLGY AGPTGVCPSP





YTCQALNIYY SQCI


 


SEQ ID NO: 94
16304152
Thermoascus
MYQRALLFSF FLAAARAHEA GTVTAENHPS LTWQQCSSGG SCTTQNGKVV IDANWRWVHT TSGYTNCYTG




aurantiacus
NTWDTSICPD DVTCAQNCAL DGADYSGTYG VTTSGNALRL NFVTQSSGKN IGSRLYLLQD DTTYQIFKLL





GQEFTFDVDV SNLPCGLNGA LYFVAMDADG NLSKYPGNKA GAKYGTGYCD SQCPRDLKFI NGQANVEGWQ





PSANDPNAGV GNHGSSCAEM DVWEANSIST AVTPHPCDTP GQTMCQGDDC GGTYSSTRYA GTCDTDGCDF





NPYQPGNHSF YGPGKIVDTS SKFTVVTQFI TDDGTPSGTL TEIKRFYVQN GKVIPQSEST ISGVTGNSIT





TEYCTAQKAA FDNTGFFTHG GLQKISQALA QGMVLVMSLW DDHAANMLWL DSTYPTDADP DTPGVARGTC





PTTSGVPADV ESQNPNSYVI YSNIKVGPIN STFTAN


 


SEQ ID NO: 95
156712280
Acremonium
MHKRAATLSA LVVAAAGFAR GQGVGTQQTE THPKLTFQKC SAAGSCTTQN GEVVIDANWR WVHDKNGYTN




thermophilum
CYTGNEWNTT ICADAASCAS NCVVDGADYQ GTYGASTSGN ALTLKFVTKG SYATNIGSRM YLMASPTKYA





MFTLLGHEFA FDVDLSKLPC GLNGAVYFVS MDEDGGTSKY PSNKAGAKYG TGYCDSQCPR DLKFIDGKAN





SASWQPSSND QNAGVGGMGS CCAEMDIWEA NSVSAAYTPH PCQNYQQHSC SGDDCGGTYS ATRFAGDCDP





DGCDWNAYRM GVHDFYGNGK TVDTGKKFSI VTQFKGSGST LTEIKQFYVQ DGRKIENPNA TWPGLEPFNS





ITPDFCKAQK QVFGDPDRFN DMGGFTNMAK ALANPMVLVL SLWDDHYSNM LWLDSTYPTD ADPSAPGKGR





GTCDTSSGVP SDVESKNGDA TVIYSNIKFG PLDSTYTAS


 


SEQ ID NO: 96
5231154
Volvariella volvacea
MRASLLAFSL NSAAGQQAGT LQTKNHPSLT SQKCRQGGCP QVNTTIVLDA NWRWTHSTSG STNCYTGNTW





QATLCPDGKT CAANCALDGA DYTGTYGVTT SGNSLTLQFV TQSNVGARLG YLMADDTTYQ MFNLLNQEFW





FDVDMSNLPC GLNGALYFSA MARTAAWMPM VVCASTPLIS TRRSTARLLR LPVPPRSRYG RGICDSQCPR





DIKFINGEAN VQGWQPSPND TNAGTGNYGA CCNKMDVWEA NSISTAYTPH PCTQRGLVRC SGTACGGGSN





RYGSICDHDG LGFQNLFGMG RTRVRARVGR VKQFNRSSRV VEPISWTKQT TLHLGNLPWK SADCNVQNGR





VIQNSKVNIP GMPSTMDSVT TEFCNAQKTA FNDTFSFQQK GGMANMSEAL RRGMVLVLSI WDDHAANMLW





LDSITSAAAC RSTPSEVHAT PLRESQIRSS HSRQTRYVTF TNIKFGPFNS TGTTYTTGSV PTTSTSTGTT





GSSTPPQPTG VTVPQGQCGG IGYTGPTTCA SPTTCHVLNP YYSQCY


 


SEQ ID NO: 97
116200349
Chaetomium
MKQYLQYLAA ALPLMSLVSA QGVGTSTSET HPKITWKKCS SGGSCSTVNA EVVIDANWRW LHNADSKNCY




globosum CBS 148-
DGNEWTDACT SSDDCTSKCV LEGAEYGKTY GASTSGDSLS LKFLTKHEYG TNIGSRFYLM NGASKYQMFT




51
LMNNEFAFDV DLSTVECGLN SALYFVAMEE DGGMASYSTN KAGAKYGTGY CDAQCARDLK FVGGKANYDG





WTPSSNDANA GVGALGGCCA EIDVWESNAH AFAFTPHACE NNNYHVCEDT TCGGTYSEDR FAGDCDANGC





DYNPYRVGNT DFYGKGMTVD TSKKFTVVSQ FQENKLTQFF VQNGKKIEIP GPKHEGLPTE SSDITPELCS





AMPEVFGDRD RFAEVGGFDA LNKALAVPMV LVMSIWDDHY ANMLWLDSSY PPEKAGTPGG DRGPCAQDSG





VPSEVESQYP DATVVWSNIR FGPIGSTVQV


 


SEQ ID NO: 98
4586343
Irpex lacteus
MFPKASLIAL SFIAAVYGQQ VGTQMAEVHP KLPSQLCTKS GCTNQNTAVV LDANWRWLHT TSGYTNCYTG





NSWDATLCPD ATTCAQNCAV DGADYSGTYG ITTSGNALTL KFKTGTNVGS RVYLMQTDTA YQMFQLLNQE





FTFDVDMSNL PCGLNGALYL SQMDQDGGLS KFPTNKAGAK YGTGYCDSQC PHDIKFINGM ANVAGWAGSA





SDPNAGSGTL GTCCSEMDIW EANNDAAAFT PHPCSVDGQT QCSGTQCGDD DERYSGLCDK DGCDFNSFRM





GDKSFLGKGM TVDTSRKFTV VTQFVTTDGT TNGDLHEIRR LYVQDGKVIQ NSVVSIPGID AVDSITDNFC





AQQKSVFGDT NYFATLGGLK KMGAALKSGM VLAMSVWDDH AASMQWLDSN YPADGDATKP GVARGTCSAD





SGLPTNVESQ SASASVTFSN IKWGDINTTF TGTGSTSPSS PAGPVSSSTS VASQPTQPAQ GTVAQWGQCG





GTGFTGPTVC ASPFTCHVVNPYYSQCY


 


SEQ ID NO: 99
15321718
Lentinula edodes
MFRTAALLSF AYLAVVYGQQ AGTSTAETHP PLTWEQCTSG GSCTTQSSSV VLDSNWRWTH VVGGYTNCYT





GNEWNTTVCP DGTTCAANCA LDGADYEGTY GISTSGNALT LKFVTASAQT NVGSRVYLMA PGSETEYQMF





NPLNQEFTFD VDVSALPCGL NGALYFSEMD ADGGLSEYPT NKAGAKYGTG YCDSQCPRDI KFIEGKANVE





GWTPSSTSPN AGTGGTGICC NEMDIWEANS ISEALTPHPC TAQGGTACTG DSCSSPNSTA GICDQAGCDF





NSFRMGDTSF YGPGLTVDTT SKITVVTQFI TSDNTTTGDL TAIRRIYVQN GQVIQNSMSN IAGVTPTNEI





TTDFCDQQKT AFGDTNTFSE KGGLTGMGAA FSRGMVLVLS IWDDDAAEML WLDSTYPVGK TGPGAARGTC





ATTSGQPDQV ETQSPNAQVV FSNIKFGAIG STFSSTGTGT GTGTGTGTGT GTTTSSAPAA TQTKYGQCGG





QGWTGATVCA SGSTCTSSGP YYSQCL


 


SEQ ID NO: 100
146424875
Pleurotus sp Florida
MFRTAALTAF TFAAVVLGQQ VGTLTTENHP ALSIQQCTAT GCTTQQKSVV LDSNWRWTHS TAGATNCYTG





NAWDPALCPD PATCATNCAI DGADYSGTYG ITTSGNALTL RFVTNGQYSQ NIGSRVYLLD DADHYKLFDL





KNQEFTFDVD MSGLPCGLNG ALYFSEMAAD GGKAAHAGNN AGAKYGTGYC DAQCPHDIKW INGEANVLDW





SASATDDNAG NGRYGACCAE MDIWEANSEA TAYTPHVCRD EGLYRCSGTE CGDGNNRYGG VCDKDGCDFN





SYRMGDKNFL GRGKTIDTTK KVTVVTQFIT DNNTPTGNLV EIRRVYVQNG VVYQNSFSTF PSLSQYNSIS





DEFCVAQKTL FGDNQYYNTH GGTTKMGDAF DNGMVLIMSL WSDHAAHMLW LDSDYPLDKS PSEPGVSRGA





CPTSSGDPDD VVANHPNASV TFSNIKYGPI GSTFGGSTPP VSSGGSSVPP VTSTTSSGTT TPTGPTGTVP





KWGQCGGIGY SGPTACVAGS TCTYSNDWYS QCL


 


SEQ ID NO: 101
62006158
Fusarium venenatum
MYRAIATASA LIAAVRAQQV CSLTPETKPA LSWSKCTSSG CSNVQGSVTI DANWRWTHQL SGSTNCYTGN





KWDTSICTSG KVCAEKCCID GAEYASTYGI TSSGNQLSLS FVTKGTYGTN IGSRTYLMED ENTYQMFQLL





GNEFTFDVDV SNIGCGLNGA LYFVSMDADG GKAKYPGNKA GAKYGTGYCD AQCPRDVKFI NGQANSDGWQ





PSKSDVNGGI GNLGTCCPEM DIWEANSIST AHTPHPCTKL TQHSCTGDSC GGTYSEDRYG GTCDADGCDF





NAYRQGNKTF YGPGSGFNVD TTKKVTVVTQ FHKGSNGRLS EITRLYVQNG KVIANSESKI AGVPGSSLTP





EFCTAQKKVF GDIDDFEKKG AWGGMSDALE APMVLVMSLW HDHHSNMLWL DSTYPTDSTK LGAQRGSCST





SSGVPADLEK NVPNSKVAFS NIKFGPIGST YKEGQPEPTN PTNPNPTTPG GTVDQWGQCG GTNYSGPTAC





KSPFTCKKIN DFYSQCQ


 


SEQ ID NO: 102
296027
Phanerochaete
MFRTATLLAF TMAAMVFGQQ VGTNTAENHR TLTSQKCTKS GGCSNLNTKI VLDANWRWLH STSGYTNCYT




chrysosporium
GNQWDATLCP DGKTCAANCA LDGADYTGTY GITASGSSLK LQFVTGSNVG SRVYLMADDT HYQMFQLLNQ





EFTFDVDMSN LPCGLNGALY LSAMDADGGM AKYPTNKAGA KYGTGYCDSQ CPRDIKFING EANVEGWNAT





SANAGTGNYG TCCTEMDIWE ANNDAAAYTP HPCTTNAQTR CSGSDCTRDT GLCDADGCDF NSFRMGDQTF





LGKGLTVDTS KPFTVVTQFI TNDGTSAGTL TEIRRLYVQN GKVIQNSSVK IPGIDLVNSI TDNFCSQQKT





AFGDTNYFAQ HGGLKQVGEA LRTGMVLALS IWDDYAANML WLDSNYPTNK DPSTPGVARG TCATTSGVPA





QIEAQSPNAY VVFSNIKFGD LNTTYTGTVS SSSVSSSHSS TSTSSSHSSS STPPTQPTGV TVPQWGQCGG





IGYTGSTTCA SPYTCHVLNP YYSQCY


 


SEQ ID NO: 103
154449709
Fusicoccum sp
MYQTSLLASL SFLLATSQAQ QVGTQTAETH PKLTTQKCTT AGGCTDQSTS IVLDANWRWL HTVDGYTNCY




BCC4124
TGQEWDTSIC TDGKTCAEKC ALDGADYEST YGISTSGNAL TMNFVTKSSQ TNIGGRVYLL AADSDDTYEL





FKLKNQEFTF DVDVSNLPCG LNGALYFSEM DSDGGLSKYT TNKAGAKYGT GYCDTQCPHD IKFINGEANV





QNWTASSTDK NAGTGHYGSC CNEMDIWEAN SQATAFTPHV CEAKVEGQYR CEGTECGDGD NRYGGVCDKD





GCDFNSYRMG NETFYGSNGS TIDTTKKFTV VTQFITADNT ATGALTEIRR KYVQNDVVIE NSYADYETLS





KFNSITDDFC AAQKTLSGDT NDFKTKGGIA RMGESFERGM VLVMSVWDDH AANALWLDSS YPTDADASKP





GVKRGPCSTS SGVPSDVEAN DADSSVIYSN IRYGDIGSTF NKTA


 


SEQ ID NO: 104
169859460
Coprinopsis cinerea
MFSKVALTAL CFLAVAQAQQ VGREVAENHP RLPWQRCTRN GGCQTVSNGQ VVLDANWRWL HVTDGYTNCY




okayama
TGNAWNSSVC SDGATCAQRC ALEGANYQQT YGITTSGDAL TIKFLTRSEQ TNIGARVYLM ENEDRYQMFN





LLNKEFTFDV DVSKVPCGIN GALYFIQMDA DGGLSSQPNN RAGAKYGTGY CDSQCPRDIK FINGEANSVG





WEPSETDPNA GKGQYGICCA EMDIWEANSI SNAYTPHPCQ TVNDGGYQRC QGRDCNQPRY EGLCDPDGCD





YNPFRMGNKD FYGPGKTVDT NRKMTVVTQF ITHDNTDTGT LVDIRRLYVQ DGRVIANPPT NFPGLMPAHD





SITQEFCDDA KRAFEDNDSF GRNGGLAHMG RSLAKGHVLA LSIWNDHTAH MLWLDSNYPT DADPNKPGIA





RGTCPTTGGS PRDTEQNHPD AQVIFSNIKF GDIGSTFSGN


 


SEQ ID NO: 105
50400675
Trichoderma
MYRKLAVISA FLAAARAQQV CTQQAETHPP LTWQKCTASG CTPQQGSVVL DANWRWTHDT KSTTNCYDGN




harzianum
TWSSTLCPDD ATCAKNCCLD GANYSGTYGV TTSGDALTLQ FVTASNVGSR LYLMANDSTY QEFTLSGNEF




(anamorph of
SFDVDVSQLP CGLNGALYFV SMDADGGQSK YPGNAAGAKY GTGYCDSQCP RDLKFINGQA NVEGWEPSSN




Hypocrea lixii)
NANTGVGGHG SCCSEMDIWE ANSISEALTP HPCETVGQTM CSGDSCGGTY SNDRYGGTCD PDGCDWNPYR





LGNTSFYGPG SSFALDTTKK LTVVTQFATD GSISRYYVQN GVKFQQPNAQ VGSYSGNTIN TDYCAAEQTA





FGGTSFTDKG GLAQINKAFQ GGMVLVMSLW DDYAVNMLWL DSTYPTNATA STPGAKRGSC STSSGVPAQV





EAQSPNSKVI YSNIRFGPIG STGGNTGSNP PGTSTTRAPP SSTGSSPTAT QTHYGQCGGT GWTGPTRCAS





GYTCQVLNPF YSQCL


 


SEQ ID NO: 106
729649
Neurospora crassa
MRASLLAFSL AAAVAGGQQA GTLTAKRHPS LTWQKCTRGG CPTLNTTMVL DANWRWTHAT SGSTKCYTGN




(OR74A)
KWQATLCPDG KSCAANCALD GADYTGTYGI TGSGWSLTLQ FVTDNVGARA YLMADDTQYQ MLELLNQELW





FDVDMSNIPC GLNGALYLSA MDADGGMRKY PTNKAGAKYA TGYCDAQCPR DLKYINGIAN VEGWTPSTND





ANGIGDHGSC CSEMDIWEAN KVSTAFTPHP CTTIEQHMCE GDSCGGTYSD DRYGVLCDAD GCDFNSYRMG





NTTFYGEGKT VDTSSKFTVV TQFIKDSAGD LAEIKAFYVQ NGKVIENSQS NVDGVSGNSI TQSFCKSQKT





AFGDIDDFNK KGGLKQMGKA LAQAMVLVMS IWDDHAANML WLDSTYPVPK VPGAYRGSGP TTSGVPAEVD





ANAPNSKVAF SNIKFGHLGI SPFSGGSSGT PPSNPSSSAS PTSSTAKPSS TSTASNPSGT GAAHWAQCGG





IGFSGPTTCP EPYTCAKDHD IYSQCV


 


SEQ ID NO: 107
119472134
Neosartorya fischeri
MLASTFSYRM YKTALILAAL LGSGQAQQVG TSQAEVHPSM TWQSCTAGGS CTTNNGKVVI DANWRWVHKV




NRRL 181
GDYTNCYTGN TWDKTLCPDD ATCASNCALE GANYQSTYGA TTSGDSLRLN FVTTSQQKNI GSRLYMMKDD





TTYEMFKLLN QEFTFDVDVS NLPCGLNGAL YFVAMDADGG MSKYPTNKAG AKYGTGYCDS QCPRDLKFIN





GQANVEGWQP SSNDANAGTG NHGSCCAEMD IWEANSISTA FTPHPCDTPG QVMCTGDACG GTYSSDRYGG





TCDPDGCDFN SFRQGNKTFY GPGMTVDTKS KFTVVTQFIT DDGTASGTLK EIKRFYVQNG KVIPNSESTW





SGVGGNSITN DYCTAQKSLF KDQNVFAKHG GMEGMGAALA QGMVLVMSLW DDHAANMLWL DSNYPTTASS





STPGVARGTC DISSGVPADV EANHPDASVV YSNIKVGPIG STFNSGGSNP GGGTTTTAKP TTTTTTAGSP





GGTGVAQHYG QCGGNGWQGP TTCASPYTCQ KLNDFYSQCL


 


SEQ ID NO: 108
117935080
Chaetomium
MQIKQYLQYL AAALPLVNMA AAQRAGTQQT ETHPRLSWKR CSSGGNCQTV NAEIVIDANW RWLHDSNYQN




thermophilum
CYDGNRWTSA CSSATDCAQK CYLEGANYGS TYGVSTSGDA LTLKFVTKHE YGTNIGSRVY LMNGSDKYQM





FTLMNNEFAF DVDLSKVECG LNSALYFVAM EEDGGMRSYS SNKAGAKYGT GYCDAQCARD LKFVGGKANI





EGWRPSTNDA NAGVGPYGAC CAEIDVWESN AYAFAFTPHG CLNNNYHVCE TSNCGGTYSE DRFGGLCDAN





GCDYNPYRMG NKDFYGKGKT VDTSRKFTVV TRFEENKLTQ FFIQDGRKID IPPPTWPGLP NSSAITPELC





TNLSKVFDDR DRYEETGGFR TINEALRIPM VLVMSIWDGH YASMLWLDSV YPPEKAGQPG AERGPCAPTS





GVPAEVEAQF PNAQVIWSNI RFGPIGSTYQ V


 


SEQ ID NO: 109
154300584
Botryotinia
MTSRIALVSL FAAVYGQQVG TYQTETHPSL TWQSCTAKGS CTTNTGSIVL DGNWRWTHGV GTSTNCYTGN




fuckeliana B05-10
TWDATLCPDD ATCAQNCALE GADYSGTYGI TTSGNSLRLN FVTQSANKNI GSRVYLMADT THYKTFNLLN





QEFTFDVDVS NLPCGLNGAV YFANLPADGG ISSTNTAGAE YGTGYCDSQC PRDMKFIKGQ ANVDGWVPSS





NNANTGVGNH GSCCAEMDIW EANSISTAVT PHSCDTVTQT VCTGDDCGGT YSSSRYAGTC DPDGCDFNSY





RMGDETFYGP GKTVDTNSVF TVVTQFLTTD GTASGTLNEI KRFYVQDGKV IPNSYSTISG VSGNSITTPF





CDAQKTAFGD PTSFSDHGGL ASMSAAFEAG MVLVLSLWDD YYANMLWLDS TYPVGKTSAG GPRGTCDTSS





GVPASVEASS PNAYVVYSNI KVGAINSTYG


 


SEQ ID NO: 110
15824271
Pseudotrichonympha
MFVFVLLWLT QSLGTGTNQA ENHPSLSWQN CRSGGSCTQT SGSVVLDSNW RWTHDSSLTN CYDGNEWSSS




grassii
LCPDPKTCSD NCLIDGADYS GTYGITSSGN SLKLVFVTNG PYSTNIGSRV YLLKDESHYQ IFDLKNKEFT





FTVDDSNLDC GLNGALYFVS MDEDGGTSRF SSNKAGAKYG TGYCDAQCPH DIKFINGEAN VENWKPQTND





ENAGNGRYGA CCTEMDIWEA NKYATAYTPH ICTVNGEYRC DGSECGDTDS GNRYGGVCDK DGCDFNSYRM





GNTSFWGPGL IIDTGKPVTV VTQFVTKDGT DNGQLSEIRR KYVQGGKVIE NTVVNIAGMS SGNSITDDFC





NEQKSAFGDT NDFEKKGGLS GLGKAFDYGM VLVLSLWDDH QVNMLWLDSI YPTDQPASQP GVKRGPCATS





SGAPSDVESQ HPDSSVTFSD IRFGPIDSTY


 


SEQ ID NO: 111
4586345
Irpex lacteus
MFRKAALLAF SFLAIAHGQQ VGTNQAENHP SLPSQKCTAS GCTTSSTSVV LDANWRWVHT TTGYTNCYTG





QTWDASICPD GVTCAKACAL DGADYSGTYG ITTSGNALTL QFVKGTNVGS RVYLLQDASN YQMFQLINQE





FTFDVDMSNL PCGLNGAVYL SQMDQDGGVS RFPTNTAGAK YGTGYCDSQC PRDIKFINGE ANVEGWTGSS





TDSNSGTGNY GTCCSEMDIW EANSVAAAYT PHPCSVNQQT RCTGADCGQG DDRYDGVCDP DGCDFNSFRM





GDQTFLGKGL TVDTSRKFTI VTQFISDDGT TSGNLAEIRR FYVQDGNVIP NSKVSIAGID AVNSITDDFC





TQQKTAFGDT NRFAAQGGLK QMGAALKSGM VLALSLWDDH AANMLWLDSD YPTTADASNP GVARGTCPTT





SGFPRDVESQ SGSATVTYSN IKWGDLNSTF TGTLTTPSGS SSPSSPASTS GSSTSASSSA SVPTQSGTVA





QWAQCGGIGY SGATTCVSPY TCHVVNAYYS QCY


 


SEQ ID NO: 112
46241268
Gibberella avenacea
MYRAIATASA LIAAARAQQV CTLTTETKPA LTWSKCTSSG CTDVKGSVGI DANWRWTHQT SSSTNCYTGN





KWDTSVCTSG ETCAQKCCLD GADYAGTYGI TSSGNQLSLG FVTKGSFSTN IGSRTYLMEN ENTYQMFQLL





GNEFTFDVDV SNIGCGLNGA LYFVSMDADG GKARYPANKA GAKYGTGYCD AQCPRDVKFI NGKANSDGWK





PSDSDINAGI GNMGTCCPEM DIWEANSIST AFTPHPCTKL TQHACTGDSC GGTYSNDRYG GTCDADGCDF





NSYRQGNKTF YGRGSDFNVD TTKKVTVVTQ FKKGSNGRLS EITRLYVQNG KVIANSESKI PGNSGSSLTA





DFCSKQKSVF GDIDDFSKKG GWSGMSDALE SPPMVLVMSL WHDHHSNMLW LDSTYPTDST KLGAQRGSCA





TTSGVPSDLE RDVPNSKVSF SNIKFGPIGS TYSSGTTNPP PSSTDTSTTP TNPPTGGTVG QYGQCGGQTY





TGPKDCKSPY TCKKINDFYS QCQ


 


SEQ ID NO: 113
6164684
Aspergillus niger
MSSFQIYRAA LLLSILATAN AQQVGTYTTE THPSLTWQTC TSDGSCTTND GEVVIDANWR WVHSTSSATN





CYTGNEWDTS ICTDDVTCAA NCALDGATYE ATYGVTTSGS ELRLNFVTQG SSKNIGSRLY LMSDDSNYEL





FKLLGQEFTF DVDVSNLPCG LNGALYFVAM DADGGTSEYS GNKAGAKYGT GYCDSQCPRD LKFINGEANC





DGWEPSSNNV NTGVGDHGSC CAEMDVWEAN SISNAFTAHP CDSVSQTMCD GDSCGGTYSA SGDRYSGTCD





PDGCDYNPYR LGNTDFYGPG LTVDTNSPFT VVTQFITDDG TSSGTLTEIK RLYVQNGEVI ANGASTYSSV





NGSSITSAFC ESEKTLFGDE NVFDKHGGLE GMGEAMAKGM VLVLSLWDDY AADMLWLDSD YPVNSSASTP





GVARGTCSTD SGVPATVEAE SPNAYVTYSN IKFGPIGSTY SSGSSSGSGS SSSSSSTTTK ATSTTLKTTS





TTSSGSSSTS AAQAYGQCGG QGWTGPTTCV SGYTCTYENA YYSQCL


 


SEQ ID NO: 114
6164682
Aspergillus niger
MHQRALLFSA LLTAVRAQQA GTLTEEVHPS LTWQKCTSEG SCTEQSGSVV IDSNWRWTHS VNDSTNCYTG





NTWDATLCPD DETCAANCAL DGADYESTYG VTTDGDSLTL KFVTGSNVGS RLYLMDTSDE GYQTFNLLDA





EFTFDVDVSN LPCGLNGALY FTAMDADGGV SKYPANKAGA KYGTGYCDSQ CPRDLKFIDG QANVDGWEPS





SNNDNTGIGN HGSCCPEMDI WEANKISTAL TPHPCDSSEQ TMCEGNDCGG TYSDDRYGGT CDPDGCDFNP





YRMGNDSFYG PGKTIDTGSK MTVVTQFITD GSGSLSEIKR YYVQNGNVIA NADSNISGVT GNSITTDFCT





AQKKAFGDED IFAEHNGLAG ISDAMSSMVL ILSLWDDYYA SMEWLDSDYP ENATATDPGV ARGTCDSESG





VPATVEGAHP DSSVTFSNIK FGPINSTFSA SA


 


SEQ ID NO: 115
33733371
Chrysosporium
MYAKFATLAA LVAGAAAQNA CTLTAENHPS LTWSKCTSGG SCTSVQGSIT IDANWRWTHR TDSATNCYEG




lucknowense
NKWDTSYCSD GPSCASKCCI DGADYSSTYG ITTSGNSLNL KFVTKGQYST NIGSRTYLME SDTKYQMFQL




U.S. Pat. No. 6,573,086-10
LGNEFTFDVD VSNLGCGLNG ALYFVSMDAD GGMSKYSGNK AGAKYGTGYC DSQCPRDLKF INGEANVENW





QSSTNDANAG TGKYGSCCSE MDVWEANNMA AAFTPHPCXV IGQSRCEGDS CGGTYSTDRY AGICDPDGCD





FNSYRQGNKT FYGKGMTVDT TKKITVVTQF LKNSAGELSE IKRFYVQNGK VIPNSESTIP GVEGNSITQD





WCDRQKAAFG DVTDXQDKGG MVQMGKALAG PMVLVMSIWD DHAVNMLWLD STWPIDGAGK PGAERGACPT





TSGVPAEVEA EAPNSNVIFS NIRFGPIGST VSGLPDGGSG NPNPPVSSST PVPSSSTTSS GSSGPTGGTG





VAKHYEQCGG IGFTGPTQCE SPYTCTKLND WYSQCL


 


SEQ ID NO: 116
29160311
Thielavia
MYAKFATLAA LVAGASAQAV CSLTAETHPS LTWQKCTAPG SCTNVAGSIT IDANWRWTHQ TSSATNCYSG




australiensis
SKWDSSICTT GTDCASKCCI DGAEYSSTYG ITTSGNALNL KFVTKGQYST NIGSRTYLME SDTKYQMFKL





LGNEFTFDVD VSNLGCGLNG ALYFVSMDAD GGMSKYSGNK AGAKYGTGYC DAQCPRDLKF INGEANVEGW





ESSTNDANAG SGKYGSCCTE MDVWEANNMA TAFTPHPCTT IGQTRCEGDT CGGTYSSDRY AGVCDPDGCD





FNSYRQGNKT FYGKGMTVDT TKKITVVTQF LKNSAGELSE IKRFYAQDGK VIPNSESTIA GIPGNSITKA





YCDAQKTVFQ NTDDFTAKGG LVQMGKALAG DMVLVMSVWD DHAVNMLWLD STYPTDQVGV AGAERGACPT





TSGVPSDVEA NAPNSNVIFS NIRFGPIGST VQGLPSSGGT SSSSSAAPQS TSTKASTTTS AVRTTSTATT





KTTSSAPAQG TNTAKHWQQC GGNGWTGPTV CESPYKCTKQ NDWYSQCL


 


SEQ ID NO: 117
146197087
uncultured symbiotic
MLTLVYFLLS LVVSLEIGTQ QSEDHPKLTW QNGSSSVSGS IVLDSNWRWV HDSGTTNCYD GNLWSKDLCP




protist of
SSDTCSQKCY IEGADYSGTY GIQSSGSKLT LKFVTKGSYS TNIGSRVYLL KDENTYESFK LKNKEFTFTV




Reticulitermes
DDSKLNCGLN GALYFVAMDA DGGKAKYSSF KPGAKYGMGY CDAQCPHDMK FISGKANVDD WKPQDNDENS




speratus
GNGKLGTCCS EMDIWEGNMK SQAYTVHACT KSGQYECTGQ QCGDTDSGDR FKGTCDKDGC DYASWRWGDQ





SFYGEGKTVD TKQPVTVVTQ FIGDPLTEIR RLYVQGGKTI NNSKTSNLAD TYDSITDKFC DATKEASGDT





NDFKAKGAMS GFSTNLNNGQ VLVMSLWDDH TANMLWLDST YPTDSSDSTA QRGPCPTSSG VPKDVESQHG





DATVVFSDIK FGAINSTFKY N


 


SEQ ID NO: 118
146197237
uncultured symbiotic
MLAAALFTFA CSVGVGTKTP ENHPKLNWQN CASKGSCSQV SGEVTMDSNW RWTHDGNGKN CYDGNTWISS




protist of Neotermes
LCPDDKTCSD KCVLDGAEYQ ATYGIQSNGT ALTLKFVTHG SYSTNIGSRL YLLKDKSTYY VFKLNNKEFT




koshunensis
FSVDVSKLPC GLNGALYFVE MDADGGKAKY AGAKPGAEYG LGYCDAQCPS DLKFINGEAN SEGWKPQSGD





KNAGNGKYGS CCSEMDVWES NSQATALTPH VCKTTGQQRC SGKSECGGQD GQDRFAGLCD EDGCDFNNWR





MGDKTFFGPG LIVDTKSPFV VVTQFYGSPV TEIRRKYVQN GKVIENSKSN IPGIDATAAI SDHFCEQQKK





AFGDTNDFKN KGGFAKLGQV FDRGMVLVLS LWDDHQVAML WLDSTYPTNK DKSQPGVDRG PCPTSSGKPD





DVESASADAT VVYGNIKFGA LDSTY


 


SEQ ID NO: 119
146197067
uncultured symbiotic
MLTLVYFLLS LVVSLEIGTQ QSEDHPKLTW QNGSSSVSGS IVLDSNWRWV HDSGTTNCYD GNLWSKDLCP




protist of
SSNTCSQKCY IEGADYSGTY GIQSSGSKLT LKFVTKGSYS TNIGSRVYLL KDENTYESFK LKNKEFTFTV




Reticulitermes
DDSKLNCGLN GALYFVAMDA DGGKAKYSSF KPGAKYGMGY CDAQCPHDMK FISGKANVDD WKPQDNDENS




speratus
GNGKLGTCCS EMDIWEGNMK SQAYTVHACT KSGQYECTGQ QCGDTDSGDR FKGTCDKDGC DYASWRWGDQ





SFYGEGKTVD TKQPVTVVTQ FIGDPLTEIR RLYVQGGKTI NNSKTSNLAD TYDSITDKFC DATKEASGDT





NDFKAKGAMS GFSTNLNNGQ VLVMSLWDDH TANMLWLDST YPTDSTKTGA SRGPCAVSSG VPKDVESQYG





DATVIYSDIK FGAINSTFKW N


 


SEQ ID NO: 120
146197407
uncultured symbiotic
MILALLSLAK SLGIATNQAE THPKLTWTRY QSKGSGQTVN GEIVLDSNWR WTHHSGTNCY DGNTWSTSLC




protist of
PDPTTCSNNC DLDGADYPGT YGISTSGNSL KLGFVTHGSY STNIGSRVYL LRDSKNYEMF KLKNKEFTFT




Cryptocercus
VDDSKLPCGL NGALYFVAMD EDGGVSKNSI NKAGAQYGTG YCDAQCPHDM KFINGEANVL DWKPQSNDEN




punctulatus
SGNGRYGACC TEMDIWEANS MATAYTPHVC TVTGLRRCEG TECGDTDANQ RYNGICDKDG CDFNSYRLGD





KTFFGVGKTV DSSKPVTVVT QFVTSNGQDS GTLSEIRRKY VQGGKVIENS KVNIAGITAG NSVTDTFCNE





QKKAFGDNND FEKKGGLGAL SKQLDAGMVL VLSLWDDHSV NMLWLDSTYP TNAAAGALGT ERGACATSSG





APSDVESQSP DATVTFSDIK FGPIDSTY


 


SEQ ID NO: 121
146197157
uncultured symbiotic
MLVIALILRG LSVGTGTQQS ETHPSLSWQQ TSKGGSGQSV SGSVVLDSNW RWTHTTDGTT NCYDGNEWSS




protist of
DLCPDASTCS SNCVLEGADY SGTYGITGSG SSLKLGFVTK GSYSTNIGSR VYLLGDESHY KLFKLENNEF




Hodotermopsis
TFTVDDSNLE CGLNGALYFV AMDEDGGASK YSGAKPGAKY GMGYCDAQCP HDMKFINGDA NVEGWKPSDN




sjoestedti
DENAGTGKWG ACCTEMDIWE ANKYATAYTP HICTKNGEYR CEGTDCGDTK DNNRYGGVCD KDGCDFNSWR





MGNQSFWGPG LIIDTGKPVT VVTQFLADGG SLSEIRRKYV QGGKVIENTV TKISGMDEFD SITDEFCNQQ





KKAFRDTNDF EKKGGLKGLG TAVDAGVVLV LSLWDDHDVN MLWLDSIYPT DSGSKAGADR GPCATSSGVP





KDVESNYASA SVTFSDIKFG PIDSTY


 


SEQ ID NO: 122
146197403
uncultured symbiotic
MLLALFAFGK SLGIATNQAE NHPKLTWTRY QSKGSGQTVN GEIVLDSNWR WTHHSGTNCY DGNTWSTSLC




protist of
PDPTTCSNNC DLDGADYPGT YGISSSGNSL KLGFVTHGSY STNIGSRVYL LRDSKNYEMF KLKNKEFTFT




Cryptocercus
VDDSKLPCGL NGALYFVAMD EDGGVSKNSI NKAGAQYGTG YCDAQCPHDM KFINGEANVL DWKPQSNDEN




punctutatus
SGNGRYGACC TEMDIWEANS MATAYTPHVC TVTGIRRCEG TECGDTDANQ RYNGICDKDG CDFNSYRLGD





KSFFGVGKTV DSSKPVTVVT QFVTSNGQDS GTLSEIRRKY VQGGKVIENS KVNIAGMAAG NSITDTFCNE





QKKAFGDNND FEKKGGLGAL SKQLDSGMVL VLSLWDDHSV NMLWLDSTYP TNAAAGALGT ERGACATSSG





APSDVESQSP DATVTFSDIK FGPIDSTY


 


SEQ ID NO: 123
146197081
uncultured symbiotic
MLASVVYLVS LVVSLEIGTQ QSEEHPKLTW QNGSSSVSGS IVLDSNWRWL HDSGTTNCYD GNLWSDDLCP




protist of
NADTCSSKCY IEGADYSGTY GITSSGSKVT LKFVTKGSYS TNIGSRIYLL KDENTYETFK LKNKEFTFTV




Reticulitermes
DDSKLDCGLN GALYFVAMDA DGGKAKYSSF KPGAKYGMGY CDAQCPHDMK FISGKANVDD WKPQDNDENS




speratus
GDGKLGTCCS EMDIWEGNAK SQAYTVHACS KSGQYECTGQ QCGDTDSGDR FKGTCDKDGC DYASWRWGDQ





SFYGEGKTVD TKSPVTVVTQ FIGDPLTEIR RVYVQGGKTI NNSKTSNLAD TYDSITDKFC DATKDATGDT





NDFKAKGAMA GFSTNLNTAQ VLVSVHCGMI IQPICCGLIR RIQRIQQKQV QAVDRVLCRR VFQRMLKASM





VMLQSRTRTL SLELSTRPLV GISPAGRLFF F


 


SEQ ID NO: 124
146197413
uncultured symbiotic
MILALLVLGK SLGIATNQAE THPKLTWTRY QSKGSGSTVN GEIVLDSNWR WTHHSGTNCY DGNTWSTSLC




protist of
PDPTTCSNNC DLDGADYPGT YGISTSGNSL KLGFVTHGSY STNIGSRVYL LKDTKSYEMF KLKNKEFTFT




Cryptocercus
VDDSKLPCGL NGALYFVAMD EDGGVSKNSI NKAGAQYGTG YCDAQCPHDM KFINGEANVL DWKPQSNDEN




punctuiatus
SGNGRYGACC TEMDIWEANS MATAYTPHVC TVTGLRRCEG TECGDTDNDQ RYNGICDKDG CDFNSYRLGD





KSFFGVGKTV DSSKPVTVVT QFVTSNGQDS GTLSEIRRKY VQGGKVIENS KVNVAGITAG NSVTDTFCNE





QKKAFGDNND FEKKGGLGAL SKQLDAGMVL VLSLWDDHSV NMLWLDSTYP TNAAAGALGT ERGACATSSG





KPSDVESQSP DATVTFSDIK FGPIDSTY


 


SEQ ID NO: 125
146197309
uncultured symbiotic
MLCIGLISFV YSLGVGTNTA ETHPKLTWKN GGQTVNGEVT VDSNWRWTHT KGSTKNCYDG NLWSKDLCPD




protist of
AATCGKNCVL EGADYSGTYG VTSSGNALTL KFVTHGSYST NVGSRLYLLK DEKTYQMFNL NGKEFTFTVD




Mastotermes
VSNLPCGLNG ALYHVNMDED GGTKRYPDNE AGAKYGTGYC DAQCPTDLKF INGIPNSDGW KPQSNDKNSG




darwiniensis
NGKYGSCCSE MDIWEANSIC SAVTPHVCDN LQQTRCQGTA CGENGGGSRF GSSCDPDGCD FNSWRMGNKT





FYGPGLIVDT KSKFTVVTQF VGNPVTEIKR KYVQNGKVIE NSYSNIEGMD KFNSVSDKFC TAQKKAFGDT





DSFTKHGGFK QLGSALAKGM VLVLSLWDDH TVNMLWLDSV YPTNSKKAGS DRGPCPTTSG VPADVESKSA





DANVIYSDIR FGAIDSTYK


 


SEQ ID NO: 126
146197227
uncultured symbiotic
MLGALVALAS CIGVGTNTPE KHPDLKWTNG GSSVSGSIVV DSNWRWTHIK GETKNCYDGN LWSDKYCPDA




protist of Neotermes
ATCGKNCVLE GADYSGTYGV TTSGDAATLK FVTHGQYSTN VGSRLYLLKD EKTYQMFNLV GKEFTFTVDV




koshunensis
SNLPCGLNGA LYFVQMDSDG GMAKYPDNQA GAKYGTGYCD AQCPTDLKFI NGIPNSDGWK PQKNDKNSGN





GKYGSCCSEM DIWEANSMAT AYTPHVCDKL EQTRCSGSAC GQNGGGDRFS SSCDPDGCDF NSWRMGNKTF





WGPGLIVDTK KPVQVVTQFV GSGGSVTEIK RKYVQGGKVI DNSMTNIAAM SKQYNSVSDE FCQAQKKAFG





DNDSFTKHGG FRQLGATLSK GHVLVLSLWD DHDVNMLWLD SVYPTNSNKP GADRGPCKTS SGVPSDVESQ





NADSTVKYSD IRFGAIDSTY SK


 


SEQ ID NO: 127
146197253
uncultured symbiotic
MLAAALFTFA CSVGVGTKTT ETHPKLNWQQ CACKGSCSQV SGEVTMDSNW RWTHDGNGKN CYDGNTWISS




protist of Neotermes
LCPDDKTCSD KCVLDGAEYQ ATYGIQSNGT ALTPKFVTHG SYSTNIGSRL YLLKDKSTYY VFQLNNKEFT




koshunensis
FSVDVSKLPC GLNGALYFVE MDADGGKSKY AGAKPGAEYG LGYCDAQCPS DLKFINGEAN SEGWKPQSGD





KNAGNGKYGS CCSEMDVWES NSMATALTPH VCKTTGQTRC SGKSECGGQD GQDRFAGNCD EDGCDFNNWR





MGDKTFFGPG LTVDTKSPFV VVTQFYGSPV TEIRRKYVQN GKVIENAKSN IPGIDATNAI SDTFCEQQKK





AFGDTNDFKN KGGFTKLGSV FSRGMVLVLS LWDDHQVAML WLDSTYPTNK DKSVPGVDRG PCPTSSGKPD





DVESASGDAT VVYGNIKFGA LDSTY


 


SEQ ID NO: 128
146197099
uncultured symbiotic
MFGFLLSLFA LQFALEIGTQ TSESHPSITW ELNGARQSGQ IVIDSNWRWL HDSGTTNCYD GNTWSSDLCP




protist of
DPEKCSQNCY LEGADYSGTY GISASGSQLT LGFVTKGSYS TNIGSRVYLL KDENTYPMFK LKNKEFTFTV




Reticulitermes
DVSNLPCGLN GALYFVAMPS DGGKAKYPLA KPGAKYGMGY CDAQCPHDMK FINGEANVLD WKPQSNDENA




speratus
GTGRYGTCCT EMDIWEANSQ ATAYTVHACS KNARCEGTEC GDDSASQRYN GICDKDGCDF NSWRWGNKTF





FGPGLTVDSS KPVTVVTQFI GDPLTEIRRI WVQGGKVIQN SFTNVSGITS VDSITNTFCD ESKVATGDTN





DFKAKGGMSG FSKALDTEVV LVLSLWDDHT ANMLWLDSTY PTDSTAIGAS RGPCATSSGD PKDVESASAN





ASVKFSDIKF GALDSTY


 


SEQ ID NO: 129
146197409
uncultured symbiotic
MLASLLPLSN SLGTASNQAE THPKLTWTQY TGKGAGQTVN GEIVLDSNWR WTHKDGTNCY DGNTWSSSLC




protist of
PDPTTCSNNC NLDGADYPGT YGITTSGNQL KLGFVTHGSY STNIGSRVYL LRDSKNYQMF KLKNKEFTFT




Cryptocercus
VDDSKLPCGL NGAVYFVAMD EDGGTAKHSI NKAGAQYGTG YCDAQCPHDM KFINGEANVL DWKPQSNDEN




punctulatus
SGNGRWGARC TEMDIWEANS RATAYTPHIC TKTGLYRCEG TECGDSDTNR YGGVCDKDGC DFNSYRMGDK





SFFGQGKTVD SSKPVTVVTQ FITDNNQDSG KLTEIRRKYV QGGKVIDNSK VNIAGITAGN PITDTFCDEA





KKAFGDNNDF EKKGGLSALG TQLEAGFVLV LSLWDDHSVN MLWLDSTYPT NASPGALGVE RGDCAITSGV





PADVESQSAD ASVTFSDIKF GPIDSTY


 


SEQ ID NO: 130
146197315
uncultured symbiotic
MLCIGLISFV YSLGVGTNTA ETHPKLTWKN GGQTVNGEVT VDSNWRWTHT KGSTKNCYDG NLWSKDLCPD




protist of
AATCGKNCVL EGADYSGTYG VTSSGNALTL KFVTHGSYST NVGSRLYLLK DEKTYQMFNL NGKEFTFTVD




Mastotermes
VSNLPCGLSG ALYHVNMDED GGTKRYPDNE AGAKYGTGYC DAQCPTDLKF INGIPNSDGW KPQSNDKNSG




darwiniensis
NGKYGSCCSE MDIWEANSIC SAVTPHVCDN LQQTRCQGAA CGENGGGSRF GSSCDPDGCD FNSWGMGNKT





FYGPGLIVDT KSKFTVVTQF VGNPVTEIKR KYVQNGKVIE NSYSNIEGMD KFNSVSDKFC TAQKKAFGDT





DSFTKHGGFK QLGSALAKGM VLVLSLWDDH TVNMLWLDSV YPTNSKKAGS DRGPCPTTSG VPADVESKSA





DANVIYSDIR FGAIDSTYK


 


SEQ ID NO: 131
146197411
uncultured symbiotic
MILALLVLGK SLGIATNQAE THPKLTWTRY QSKGSGSTVN GEIVLDSNWR WTHHSGTNCY DGNTWSTSLC




protist of
PDPTTCSNNC DLDGADYPGT YGISTSGNSL KLGFVTHGSY STNIGSRVYL LRDSKNYEMF KLKNKEFTFT




Cryptocercus
VDDSKLPCGL NGALYFVAMD EDGGVSKNSI NKAGAQYGTG YCDAQCPHDM KFINGEANVL DWKPQSNDEN




punctulatus
SGNGRYGACC TEMDIWEANS MATAYTPHVC TVTGLRRCEG TECGDTDNDQ RYNGICDKDG CDFNSYRLGD





KSFFGVGKTV DSSKPVTVVT QFVTSNGQDS GILSETRRKY VQGGKVIENS KVNVAGITAG NSVTDTFCNE





QKKAFGDNND FEKKGGLGAL SKQLDAGMVL VLSLWDDHSV NMLWLDSTYP TNAAAGALGT ERGACATSSG





KPSDVESQSP DATVTFSDIK FGPIDSTY


 


SEQ ID NO: 132
146197161
uncultured symbiotic
MIGIVLIQTV FGIGVGTQQS ESHPSLSWQQ CSKGGSCTSV SGSIVLDSNW RWTHIPDGTT NCYDGNEWSS




protist of
DLCPDPTTCS NNCVLEGADY SGTYGISTSG SSAKLGFVTK GSYSTNIGSR VYLLGDESHY KIFDLKNKEF




Hodotermopsis
TFTVDDSNLE CGLNGALYFV AMDEDGGASR FTLAKPGAKY GTGYCDAQCP HDIKFINGEA NVQDWKPSDN




sjoestedti
DDNAGTGHYG ACCTEMDIWE ANKYATAYTP HICTENGEYR CEGKSCGDSS DDRYGGVCDK DGCDFNSWRL





GNQSFWGPGL IIDTGKPVTV VTQFVTKDGT DSGALSEIRR KYVQGGKTIE NTVVKISGID EVDSITDEFC





NQQKQAFGDT NDFEKKGGLS GLGKAFDYGV VLVLSLWDDH DVNMLWLDSV YPTNPAGKAG ADRGPCATSS





GDPKEVEDKY ASASVTFSDI KFGPIDSTY


 


SEQ ID NO: 133
146197323
uncultured symbiotic
MLVFGIVSFV YSIGVGTNTA ETHPKLTWKN GGSTTNGEVT VDSNWRWTHT KGSTKNCYDG NLWSKDLCPD




protist of
AATCGKNCVL EGADYSGTYG VTSSGDALTL KFVTHGSYST NVGSRLYLLK DEKTYQMFNL NGKEFTFTVD




Mastotermes
VSQLPCGLNG ALYFVCMDQD GGMSRYPDNQ AGAKYGTGYC DAQCPTDLKF INGLPNSDGW KPQSNDKNSG




darwiniensis
NGKYGSCCSE MDIWEANSLA TAVTPHVCDQ VGQTRCEGRA CGENGGGDRF GSICDPDGCD FNSWRMGNKT





FWGPGLIIDT KKPVTVVTQF IGSPVTEIKR EYVQGGKVIE NSYTNIEGMD KFNSISDKFC TAQKKAFGDN





DSFTKHGGFS KLGQSFTKGQ VLVLSLWDDH TVNMLWLDSV YPTNSKKLGS DRGPCPTSSG VPADVESKNA





DSSVKYSDIR FGSIDSTYK


 


SEQ ID NO: 134
146197077
uncultured symbiotic
MLSFVFLLGF GVSLEIGTQQ SENHPTLSWQ QCTSSGSCTS QSGSIVLDSN WRWVHDSGTT NCYDGNEWSS




protist of
DLCPDPETCS KNCYLDGADY SGTYGITSNG SSLKLGFVTE GSYSTNIGSR VYLKKDTNTY QIFKLKNHEF




Reticulitermes
TFTVDVSNLP CGLNGALYFV EMEADGGKGK YPLAKPGAQY GMGYCDAQCP HDMKFINGNA NVLDWKPQET




speratus
DENSGNGRYG TCCTEMDIWE ANSQATAYTP HICTKDGQYQ CEGTECGDSD ANQRYNGVCD KDGCDFNSYR





LGNKTFFGPG LIVDSKKPVT VVTQFITSNG QDSGDLTEIR RIYVQGGKTI QNSFTNIAGL TSVDSITEAF





CDESKDLFGD TNDFKAKGGF TAMGKSLDTG VVLVLSLWDD HSVNMLWLDS TYPTDAAAGA LGTQRGPCAT





SSGAPSDVES QSPDASVTFS DIKFGPLDST Y


 


SEQ ID NO: 135
146197089
uncultured symbiotic
MLTLVVYLLS LVVSLEIGTQ QSESHPALTW QREGSSASGS IVLDSNWRWV HDSGTTNCYD GNEWSTDLCP




protist of
SSDTCTQKCY IEGADYSGTY GITTSGSKLT LKFVTKGSYS TNIGSRVYLL KDENTYETFK LKNKEFTFTV




Reticulitermes
DDSKLDCGLN GALYFVAMDA DGGKQKYSSF KPGAKYGMGY CDAQCPHDMK FISGKANVED WKPQDNDENS




speratus
GNGKLGTCCS EMDIWEGNAK SQAYTVHACT KSGQYECTGT DCGDSDSRYQ GTCDKDGCDY ASYRWGDHSF





YGEGKTVDTK QPITVVTQFI GDPLTEIRRL YIQGGKVINN SKTQNLASVY DSITDAFCDA TKAASGDTND





FKAKGAMAGF SKNLDTPQVL VLSLWDDHTA NMLWLDSTYP TDSRDATAER GPCATSSGVP KDVESNQADA





SVVFSDIKFG AINSTYSYN


 


SEQ ID NO: 136
146197091
uncultured symbiotic
MFGFLLSLFA LQFALEIGTQ TSESHPSITW ELNGARQSGQ IVIDSNWRWL HDSGTTNCYD GNTWSSDLCP




protist of
DPEKCSQNCY LEGADYSGTY GISASGSQLT LGFVTKGSYS TNIGSRVYLL KDENTYQMFK LKNKEFTFTV




Reticulitermes
DVSNLPCGLN GALYFVAMPS DGGKAKYPLA KPGAKYGMGY CDAQCPHDMK FINGEANVLD WKPQSNDENA




speratus
GTGRYGTCCT EMDIWEANSQ ATAYTVHACS KNARCEGTEC GDDSASQRYN GICDKDGCDF NSWRWGNKTF





FGPGLTVDSS KPVTVVTQFI GDPLTEIRRI WVQGGKVIQN SFTNVSGITS VDSITNTFCD ESKVATGDTN





DFKAKGGMSG FSKALDTEVV LVLSLWDDHT ANMLWLDSTY PSNSTAIGAT RGPCATSSGD PKNVESASAN





ASVKFSDIKF GAFDSTY


 


SEQ ID NO: 137
146197097
uncultured symbiotic
MLALVYFLLS LVVSLEIGTQ QSEDHPKLTW QNGSSSVSGS IVLDSNWRWV HDSGTTNCYD GNLWSTDLCP




protist of
SSDTCTSKCY IEGADYSGTY GITSSGSKVT LKFVTKGSYS TNIGSRIYLL KDENTYETFK LKNKEFTFTV




Reticulitermes
DDSQLNCGLN GALYFVAMDA DGGKAKYSSF KPGAKYGMGY CDAQCPHDMK FISGKANVDD WKPQDNDENS




speratus
GNGKLGTCCS EMDIWEGNAK SQAYTVHACT KSGQYECTGQ QCGDTDSGDR FKGTCDKDGC DYASWRWGDQ





SFYGEGKTVD TKQPVTVVTQ FIGDPLTEIR RLYVQGGKTI NNSKTSNLAD TYDSITDKFC DATKEASGDT





NDFKAKGAMS GFSTNLNTAQ VLVLSLWDDH TANMLWLDST YPTDSTKTGA SRGPCAVTSG VPKDVESQYG





SAQVVYSDIK FGAINSTY


 


SEQ ID NO: 138
146197095
uncultured symbiotic
MLALVYFLLS FVVSLEIGTQ QSEDHPKLTW QNGSSSVSGS IVLDSNWRWV HDSGTTNCYD GNLWSTDLCG




protist of
SSDTCSSKCY IEGADYSGTY GISASGSKLT LKFVTKGSYS TNIGSRVYLL KDENTYETFK LKGKEFTFTV




Reticulitermes
DDSKLDCGLN GALYFVAMDA DGGKAKYSSF KPGAKYGMGY CDAQCPHDMK FISGKANVDD WKPQDNDENS




speratus
GNGKLGTCCS EMDIWEGNAK SQAYTVHACT KSGQYECTGQ QCGDTDSGDR FKGTCDKDGC DYASWRWGDQ





SFYGEGKTID TKQPVTVVTQ FIGDPLTEIR RVYVQGGKVI NNSKTSNLAN VYDSITDKFC DDTKDATGDT





NDFKAKGAMS GFSTNLNTAQ VLVMSLWDDH TANMLWLDST YPTDSTKTGA SRGPCAVLSG VPKNVESQHG





DATVIYSDIK FGAINSTFSY N


 


SEQ ID NO: 139
146197401
uncultured symbiotic
MFLALFVLGK SLGIATNQAE NHPKLTWTRY QSKGSGQTVN GEVVLDSNWR WTHHSGTNCY DGNTWSTSLC




protist of
PDPQTCSSNC DLDGADYPGT YGISSSGNSL KLGFVTHGSY STNIGSRVYL LRDSKNYEMF KLKNKEFTFT




Cryptocercus
VDDSKLPCGL NGALYFVAME EDGGVAKNSI NKAGAQYGTG YCDAQCPHDM KFINGEANVL DWKPQSNDEN




punctulatus
SGNGRYGACC IEMDIWEANS MATAYTPHVC TVTGIHRCEG TECGDTDANQ RYNGICDKDG CDFNSYRMGD





KSFFGVGKTV DSSKPVTVVT QFVTSNGQDG GTLSEIKRKY VQGGKVIENS KVNIAGITAV NSITDTFCNE





QKKAFGDNND FEKKGGLGAL SKQLDLGMVL VLSLWDDHSV NMLWLDSTYP TDAAAGALGT ERGACATSSG





KPSDVESQSP DASVTFSDIK FGPIDSTY


 


SEQ ID NO: 140
146197225
uncultured symbiotic
MLLCLLSIAN SLGVGTNTAE NHPKLSWKNG GSSVSGSVTV DANWRWTHIK GETKNCYDGN LWSDKYCPDA




protist of Neotermes
ATCGKNCVIE GADYQGTYGV SSSGDGLTLT FVTHGQYSTN VGSRLYLMKD EKTYQMFNLN GKEFTFTVDV




koshunensis
SNLPCGLNGA LYFVQMDSDG GMAKYPDNQA GAKYGTGYCD AQCPTDLKFI NGIPNSDGWK PQKNDKNSGN





GKYGSCCSEM DIWEANSQAT AYTPHVCDKL EQTRCSGSSC GHTGGGERFS SSCDPDGCDF NSWRMGNKTF





WGPGLIVDTK KPVQVVTQFV GSGNSCTEIK RKYVQGGKVI DNSMSNIAGM SKQYNSVSDD FCQAQKKAFG





DNDSFTKHGG FRQLGATLGK GHVLVLSLWD DHDVNMLWLD SVYPTNSNKP GSDRGPCKTS SGIPADVESQ





AASSSVKYSD IRFGAIDSTY K


 


SEQ ID NO: 141
146197317
uncultured symbiotic
MLCIGLISFV YSLGVGTNTA ETHPKLTWKN GGQTVNGEVT VDSNWRWTHT KGSTKNCYDG NLWSKDLCPD




protist of
AATCGKNCVL EGADYSGTYG VTSSGNALTL KFVTHGSYST NVGSRLYLMK DEKTYQMFNL NGKEFTFTVD




Mastotermes
VSNLPCGLNG ALYHVNMDED GGTKRYPDNE AGAKYGTGYC DAQCPTDLKF INGIPNSDGW KPQSNDKNSG




darwiniensis
NGKYGSCCSE MDIWEANSIC SAVTPHVCDT LQQTRCQGTA CGENGGGSRF GSSCDPDGCD FNSWRMGNKT





FYGPGLIVDT KSKFTVVTQF VGSPVTEIKR KYVQNGKVIE NSFSNIEGMD KFNSISDKFC TAQKKAFGDT





DSFTKHGGFK QLGSALAKGM VLVLSLWDDH TVNMLWLDSV YPTNSKKAGS DRGPCPTTSG VPADVESKSA





NANVIYSDIR FGAIDSTYK


 


SEQ ID NO: 142
146197251
uncultured symbiotic
MLLCLLGIAS SLDAGTNTAE NHPQLSWKNG GSSVSGSVTV DANWRWTHIK GETKNCYDGN LWSDKYCPDA




protist of Neotermes
ATCGQNCVIE GADYQGTYGV SASGNALTLT FVTHGQYSTN VGSRLYLLKD EKTYQIFNLI GKEFTFTVDV




koshunensis
SNLPCGLNGA LYFVQMDADG GTAKYSDNKA GAKYGTGYCD AQCPTDLKFI NGIPNSDGWK PQKNDKNSGN





GRYGSCCSEM DVWEANSLAT AYTPHVCDKL EQVRCDGRAC GQNGGGDRFS SSCDPDGCDF NSWRLGNKTF





WGPGLIVDTK QPVQVVTQWV GSGTSVTEIK RKYVQGGKVI DNSFTKLDSL TKQYNSVSDE FCVAQKKAFG





DNDSFTKHGG FRQLGATLAK GHVLVLSLWD DHDVNMLWLD SVYPTNSNKP GADRGPCKTS SGVPADVESQ





AASSSVKYSD IRFGAIDSTY K


 


SEQ ID NO: 143
146197319
uncultured symbiotic
MLGIGFVCIV YSLGVGTNTA ENHPKLTWKN SGSTTNGEVT VDSNWRWTHT KGTTKNCYDG NLWSKDLCPD




protist of
AATCGKNCVL EGADYSGTYG VTSSGDALTL KFVTHGSYST NVGSRLYLLK DEKTYQIFNL NGKEFTFTVD




Mastotermes
VSNLPCGLNG ALYFVNMDAD GGTGRYPDNQ AGAKYGTGYC DAQCPTDLKF INGIPNSDGW KPQSNDKNSG




darwiniensis
NGKYGSCCSE MDIWEANSLA TAVTPHVCDQ VGQTRCEGRA CGENGGGDRF GSSCDPDGCD FNSWRLGNKT





FWGPGLIVDT KKPVTVVTQF VGSPVTEIKR KYVQGGKVIE NSYTNIEGLD KFNSISDKFC TAQKKAFGDN





DSFIKHGGFR QLGQSFTKGQ VLVLSLWDDH TVNMLWLDSV YPTNSKKPGA DRGPCPTSSG VPADVESKNA





GSSVKYSDIR FGSIDSTYK


 


SEQ ID NO: 144
146197071
uncultured symbiotic
MATLVGILVS LFALEVALEI GTQTSESHPS LSWELNGQRQ TGSIVIDSNW RWLHDSGTTN CYDGNEWSSD




protist of
LCPDPEKCSQ NCYLEGADYS GTYGISSSGN SLQLGFVTKG SYSTNIGSRV YLLKDENTYA TFKLKNKEFT




Reticulitermes
FTADVSNLPC GLNGALYFVA MPADGGKSKY PLAKPGAKYG MGYCDAQCPH DMKFINGEAN ILDWKPSSND




speratus
ENAGAGRYGT CCTEMDIWEA NSQATAYTVH ACSKNARCEG TECGDDDGRY NGICDKDGCD FNSWRWGNKT





FFGPNLIVDS SKPVTVVTQF IGDPLTEIRR IYVQGGKVIQ NSFTNISGVA SVDSITDAFC NENKVATGDT





NDFKAKGGMS GFSKALDTEV VLVLSLWDDH TANMLWLDST YPTDSSALGA SRGPCAITSG EPKDVESASA





NASVKFSDIK FGAIDSTY


 


SEQ ID NO: 145
146197075
uncultured symbiotic
MLTLVYFLLS LVVSLEIGTQ QSESHPQLSW QNGSSSVSGS IVLDSNWRWV HDSGTTNCYD GNLWSTDLCP




protist of
SSDTCTSKCY IEGADYSGTY GITSSGSKLT LKFVTKGSYS TNIGSRVYLL KDENTYETFK LKNKEFTFTV




Reticulitermes
DDSKLDCGLN GALYFVAMDA DGGKAKYSSF KPGAKYGMGY CDAQCPHDMK FISGKANVDD WKPQDNDENS




speratus
GNGKLGTCCS EMDIWEGNAK SQAYTVHACT KSGQYECTGQ QCGDTDSGDR FKGTCDKDGC DYASWRWGDQ





SFYGEGKTVD TKQPLTVVTQ FVGDPLTEIR RVYVQGGKTI NNSKTSNLAD TYDSITDKFC DATKEASGDT





NDFKAKGAMS GFSTNLNTAQ VLVMSLWDDH TANMLWLDST YPTDSTKTGA SRGPCAVSSG VPKDVESQHG





DATVIYSDIK FGAINSTFKW N


 


SEQ ID NO: 146
146197159
uncultured symbiotic
MLSLVSIFLV GLGFSLGVGT QQSESHPSLS WQNCSAKGSC QSVSGSIVLD SNWRWLHDSG TTNCYDGNEW




protist of
STDLCPDAST CDKNCYIEGA DYSGTYGITS SGAQLKLGFV TKGSYSTNIG SRVYLLRDES HYQLFKLKNH




Hodotermopsis
EFTFTVDDSQ LPCGLNGALY FVEMAEDGGA KPGAQYGMGY CDAQCPHDMK FITGEANVKD WKPQETDENA




sjoestedti
GNGHYGACCT EMDIWEANSQ ATAYTPHICS KTGIYRCEGT ECGDNDANQR YNGVCDKDGC DFNSYRLGNK





TFWGPGLTVD SNKAMIVVTQ FTTSNNQDSG ELSEIRRIYV QGGKTIQNSD TNVQGITTTN KITQAFCDET





KVTFGDTNDF KAKGGFSGLS KSLESGAVLV LSLWDDHSVN MLWLDSTYPT DSAGKPGADR GPCAITSGDP





KDVESQSPNA SVTFSDIKFG PIDSTY


 


SEQ ID NO: 147
146197405
uncultured symbiotic
MILALLVLGK SLGIATNQAE THPKLTWTRY QSKGSGSTVN GEIVLDSNWR WTHHSGTNCY DGNTWSTSLC




protist of
PDPTTCSNNC DLDGADYPGT YGISTSGNSL KLGFVTHGSY STNIGSRVYL LKDTKSYEMF KLKNKEFTFT




Cryptocercus
VDDSKLPCGL NGALYFVAMD EDGGVSKNSI NKAGAQYGTG YCDAQCPHDM KFINGEANVL DWKPQSNDEN




punctuiatus
SGNGRYGACC TEMDIWEANS MATAYTPHVC TVTGLRRCEG TECGDTDNDQ RYNGICDKDG CDFNSYRLGD





KSFFGVGKTV DSSKPVTVVT QFVTSNGQDS GTLSEIRRKY VQGGKVIENS KVNVAGITAG NSVTDTFCNE





QKKAFGDNND FEKKGGFGAL SKQLVAGMVL VLSLWDDHSV NMLWLDSTYP TNAAAGALGT ERGACATSSG





KPSDVESQSP DATVTFSDIK FGPIDSTY


 


SEQ ID NO: 148
146197327
uncultured symbiotic
MLCVGLFGLV YSIGVGTNTQ ETHPKLSWKQ CSSGGSCTTQ QGSVVIDSNW RWTHSTKDLT NCYDGNLWDS




protist of
TLCPDGTTCS KNCVLEGADY SGTYGITSSG DSLTLKFVTH GSYSTNVGSR LYLLKDDNNY QIFNLAGKEF




Mastotermes
TFTVDVSNLP CGLNGALYFV EMDQDGGKGK HKENEAGAKY GTGYCDAQCP TDLKFIDGIA NSDGWKPQDN




darwiniensis
DENSGNGKYG SCCSEMDIWE ANSLATAYTP HVCDTKGQKR CQGTACGENG GGDRFGSECD PDGCDFNSWR





QGNKSFWGPG LIIDTKKSVQ VVTQFIGSGS SVTEIRRKYV QNGKVIENSY STISGTEKYN SISDDYCNAQ





KKAFGDTNSF ENHGGFKRFS QHIQDMVLVL SLWDDHTVNM LWLDSVYPTN SNKPGADRGP CETSSGVPAD





VESKSASASV KYSDIRFGPI DSTYK


 


SEQ ID NO: 149
146197261
uncultured symbiotic
MLLCLWSIAY SLGVGTNTAE NHPKLSWKNG GSSVSGSVTV DANWRWTHIK GETKNCYDGN LWSDKYCPDA




protist of Neotermes
ATCGKNCVIE GADYQGTYGV SASGDGLTLT FVTHGQYSTN VGSRLYLMKD EKTYQIFNLN GKEFTFTVDV




koshunensis
SNLPCGLNGA LYFVQMDSDG GMAKYPDNQA GAKYGTGYCD AQCPTDLKFI NGIPNSDGWK PQKNDKNSGN





GKYGSCCSEM DIWEANSQAT AYTPHVCDKL EQTRCSGSAC GHTGGGERFS SSCDPDGCDF NSWRMGNKTF





WGPGLIVDTK KPVQVVTQFV GSGNSCTEIK RKYVQGGKVI DNSMSNIAGM TKQYNSVSDD FCQAQKKAFG





DNDSFTKHGG FRQLGATLGK GHVLVLSLWD DHDVNMLWLD SVYPTNSNKP GSDRGPCKTS SGIPADVESQ





AASSSVKYSD IRFGAIDSTY K




















TABLE 2





Sequence
Database

Position
Position


Identifier
Accession

corresponding
corresponding


(SEQ ID NO:)
Number
Species of Origin
to position 268
to position 411







SEQ ID NO: 1
BD29555*
Unknown
273
422


SEQ ID NO: 2
340514556
Trichoderma reesei
268
411


SEQ ID NO: 3
51243029
Penicillium occitanis
273
422


SEQ ID NO: 4
7cel (PDB) &
Trichoderma reesei
251
394


SEQ ID NO: 5
67516425
Aspergillus nidulans FGSC A4
274
424


SEQ ID NO: 6
46107376
Gibberella zeae PH-1
268
415


SEQ ID NO: 7
70992391
Aspergillus fumigatus Af293
277
427


SEQ ID NO: 8
121699984
Aspergillus clavatus NRRL 1
277
427


SEQ ID NO: 9
1906845
Claviceps purpurea
269
416


SEQ ID NO: 10
1gpi (PDB) &
Phanerochaete chrysosporium
240
391


SEQ ID NO: 11
119468034
Neosartorya fischeri NRRL 181
265
414


SEQ ID NO: 12
7804883
Leptosphaeria maculans
256
401


SEQ ID NO: 13
85108032
Neurospora crassa N150
268
412


SEQ ID NO: 14
169859458
Coprinopsis cinerea okayama
270
421


SEQ ID NO: 15
154292161
Botryotinia fuckeliana B05-10
—
410


SEQ ID NO: 16
169615761 #
Phaeosphaeria nodorum SN15
246
393


SEQ ID NO: 17
4883502
Humicola grisea
272
413


SEQ ID NO: 18
950686
Humicola grisea
270
416


SEQ ID NO: 19
124491660
Chaetomium thermophilum
272
413


SEQ ID NO: 20
58045187
Chaetomium thermophilum
270
416


SEQ ID NO: 21
169601100 #
Phaeosphaeria nodorum SN15
237
383


SEQ ID NO: 22
169870197
Coprinopsis cinerea okayama
269
421


SEQ ID NO: 23
3913806
Agaricus bisporus
263
414


SEQ ID NO: 24
169611094
Phaeosphaeria nodorum SN15
270
414


SEQ ID NO: 25
3131
Phanerochaete chrysosporium
—
410


SEQ ID NO: 26
70991503
Aspergillus fumigatus Af293
265
414


SEQ ID NO: 27
294196
Phanerochaete chrysosporium
258
409


SEQ ID NO: 28
18997123
Thermoascus aurantiacus
268
418


SEQ ID NO: 29
4204214
Humicola grisea var thermoidea
272
413


SEQ ID NO: 30
34582632
Trichoderma viride (also known
268
411




as Hypochrea rufa)




SEQ ID NO: 31
156712284
Thermoascus aurantiacus
268
418


SEQ ID NO: 32
39977899
Magnaporthe grisea (oryzae) 70-
268
414




15




SEQ ID NO: 33
20986705
Talaromyces emersonii
266
416


SEQ ID NO: 34
22138843
Aspergillus oryzae
265
414


SEQ ID NO: 35
55775695
Penicillium chrysogenum
276
426


SEQ ID NO: 36
171676762
Podospora anserina
270
417


SEQ ID NO: 37
146350520
Pleurotus sp Florida
268
420


SEQ ID NO: 38
37732123
Gibberella zeae
268
415


SEQ ID NO: 39
156055188
Sclerotinia sclerotiorum 1980
—
410


SEQ ID NO: 40
453224
Phanerochaete chrysosporium
258
409


SEQ ID NO: 41
50402144
Trichoderma reesei
268
411


SEQ ID NO: 42
115397177
Aspergillus terreus NIH2624
274
424


SEQ ID NO: 43
154312003
Botryotinia fuckeliana B05-10
266
416


SEQ ID NO: 44
49333365
Volvariella volvacea
268
420


SEQ ID NO: 45
729650
Penicillium janthinellum
274
424


SEQ ID NO: 46
146424871
Pleurotus sp Florida
267
418


SEQ ID NO: 47
67538012
Aspergillus nidulans FGSC A4
265
410


SEQ ID NO: 48
62006162
Fusarium poae
268
415


SEQ ID NO: 49
146424873
Pleurotus sp Florida
267
418


SEQ ID NO: 50
295937
Trichoderma viride
268
411


SEQ ID NO: 51
6179889 #
Alternaria alternata
240
386


SEQ ID NO: 52
119483864
Neosartorya fischeri NRRL 181
278
428


SEQ ID NO: 53
85083281
Neurospora crassa OR74A
270
412


SEQ ID NO: 54
3913803
Cryphonectria parasitica
269
416


SEQ ID NO: 55
60729633
Corticium rolfsii
265
415


SEQ ID NO: 56
39971383
Magnaporthe grisea 70-15
268
410


SEQ ID NO: 57
39973029
Magnaporthe grisea 70-15
269
410


SEQ ID NO: 58
1170141
Fusarium oxysporum
268
415


SEQ ID NO: 59
121710012
Aspergillus clavatus NRRL 1
265
414


SEQ ID NO: 60
17902580
Penicillium funiculosum
273
422


SEQ ID NO: 61
1346226
Humicola grisea var thermoidea
270
416


SEQ ID NO: 62
156712282
Chaetomium thermophilum
270
416


SEQ ID NO: 63
169768818
Aspergillus oryzae RIB40
277
427


SEQ ID NO: 64
46241270
Gibberella pulicaris
268
415


SEQ ID NO: 65
49333363
Volvariella volvacea
265
418


SEQ ID NO: 66
46395332
Irpex lacteus
263
414


SEQ ID NO: 67
50844407 #
Chaetomium thermophilum var
245
391




thermophilum




SEQ ID NO: 68
4586347
Irpex lacteus
264
415


SEQ ID NO: 69
3980202
Phanerochaete chrysosporium
258
410


SEQ ID NO: 70
27125837
Melanocarpus albomyces
273
414


SEQ ID NO: 71
171696102
Podospora anserina
265
415


SEQ ID NO: 72
3913802
Cochliobolus carbonum
270
416


SEQ ID NO: 73
50403723
Trichoderma viride
268
411


SEQ ID NO: 74
3913798
Aspergillus aculeatus
275
425


SEQ ID NO: 75
66828465
Dictyostelium discoideum
269
419


SEQ ID NO: 76
156060391
Sclerotinia sclerotiorum 1980
252
402


SEQ ID NO: 77
116181754
Chaetomium globosum CBS
263
413




148-51




SEQ ID NO: 78
145230535
Aspergillus niger
274
424


SEQ ID NO: 79
46241266
Nectria haematococca mpVI
268
415


SEQ ID NO: 80
1q9h (PDB) #
Talaromyces emersonii
248
398


SEQ ID NO: 81
157362170
Polyporus arcularius
269
420


SEQ ID NO: 82
7804885
Leptosphaeria maculans
267
407


SEQ ID NO: 83
121852
Phanerochaete chrysosporium
258
409


SEQ ID NO: 84
126013214
Penicillium decumbens
264
415


SEQ ID NO: 85
156048578
Sclerotinia sclerotiorum 1980
265
413


SEQ ID NO: 86
156712278
Acremonium thermophilum
269
414


SEQ ID NO: 87
21449327
Aspergillus nidulans
265
410


SEQ ID NO: 88
171683762
Podospora anserina
274
415


SEQ ID NO: 89
56718412
Thermoascus aurantiacus var
268
418




levisporus




SEQ ID NO: 90
15824273
Pseudotrichonympha grassii
263
414


SEQ ID NO: 91
115390801
Aspergillus terreus NIH2624
266
411


SEQ ID NO: 92
453223
Phanerochaete chrysosporium
258
409


SEQ ID NO: 93
3132
Phanerochaete chrysosporium
—
407


SEQ ID NO: 94
16304152
Thermoascus aurantiacus
268
417


SEQ ID NO: 95
156712280
Acremonium thermophilum
273
420


SEQ ID NO: 96
5231154
Volvariella volvacea
281
438


SEQ ID NO: 97
116200349
Chaetomium globosum CBS 
270
412




148-51




SEQ ID NO: 98
4586343
Irpex lacteus
263
414


SEQ ID NO: 99
15321718
Lentinula edodes
—
417


SEQ ID NO: 100
146424875
Pleurotus sp Florida
267
418


SEQ ID NO: 101
62006158
Fusarium venenatum
268
415


SEQ ID NO: 102
296027
Phanerochaete chrysosporium
258
409


SEQ ID NO: 103
154449709
Fusicoccum sp BCC4124
272
424


SEQ ID NO: 104
169859460
Coprinopsis cinerea okayama
269
421


SEQ ID NO: 105
50400675
Trichoderma harzianum
264
407


SEQ ID NO: 106
729649
Neurospora crassa
262
406


SEQ ID NO: 107
119472134
Neosartorya fischeri NRRL 181
277
427


SEQ ID NO: 108
117935080
Chaetomium thermophilum
272
413


SEQ ID NO: 109
154300584
Botryotinia fuckeliana B05-10
265
413


SEQ ID NO: 110
15824271
Pseudotrichonympha grassii
263
414


SEQ ID NO: 111
4586345
Irpex lacteus
263
414


SEQ ID NO: 112
46241268
Gibberella avenacea
268
416


SEQ ID NO: 113
6164684
Aspergillus niger
274
424


SEQ ID NO: 114
6164682
Aspergillus niger
266
412


SEQ ID NO: 115
33733371
Chrysosporium lucknowense
269
415




US6573086-10




SEQ ID NO: 116
29160311
Thielavia australiensis
269
415


SEQ ID NO: 117
146197087
uncultured symbiotic protist of
260
402




Reticulitermes speratus




SEQ ID NO: 118
146197237
uncultured symbiotic protist of
264
409




Neotermes koshunensis




SEQ ID NO: 119
146197067
uncultured symbiotic protist of
260
402




Reticulitermes speratus




SEQ ID NO: 120
146197407
uncultured symbiotic protist of
261
412




Cryptocercus punctulatus




SEQ ID NO: 121
146197157
uncultured symbiotic protist of
264
410




Hodotermopsis sjoestedti




SEQ ID NO: 122
146197403
uncultured symbiotic protist of
261
412




Cryptocercus punctulatus




SEQ ID NO: 123
146197081
uncultured symbiotic protist of
260
410




Reticulitermes speratus




SEQ ID NO: 124
146197413
uncultured symbiotic protist of
261
412




Cryptocercus punctulatus




SEQ ID NO: 125
146197309
uncultured symbiotic protist of
259
402




Mastotermes darwiniensis




SEQ ID NO: 126
146197227
uncultured symbiotic protist of
258
404




Neotermes koshunensis




SEQ ID NO: 127
146197253
uncultured symbiotic protist of
264
409




Neotermes koshunensis




SEQ ID NO: 128
146197099
uncultured symbiotic protist of
258
401




Reticulitermes speratus




SEQ ID NO: 129
146197409
uncultured symbiotic protist of
260
411




Cryptocercus punctulatus




SEQ ID NO: 130
146197315
uncultured symbiotic protist of
259
402




Mastotermes darwiniensis




SEQ ID NO: 131
146197411
uncultured symbiotic protist of
261
412




Cryptocercus punctulatus




SEQ ID NO: 132
146197161
uncultured symbiotic protist of
263
413




Hodotermopsis sjoestedti




SEQ ID NO: 133
146197323
uncultured symbiotic protist of
259
402




Mastotermes darwiniensis




SEQ ID NO: 134
146197077
uncultured symbiotic protist of
264
415




Reticulitermes speratus




SEQ ID NO: 135
146197089
uncultured symbiotic protist of
258
400




Reticulitermes speratus




SEQ ID NO: 136
146197091
uncultured symbiotic protist of
258
401




Reticulitermes speratus




SEQ ID NO: 137
146197097
uncultured symbiotic protist of
260
402




Reticulitermes speratus




SEQ ID NO: 138
146197095
uncultured symbiotic protist of
260
402




Reticulitermes speratus




SEQ ID NO: 139
146197401
uncultured symbiotic protist of
261
412




Cryptocercus punctulatus




SEQ ID NO: 140
146197225
uncultured symbiotic protist of
258
404




Neotermes koshunensis




SEQ ID NO: 141
146197317
uncultured symbiotic protist of
259
402




Mastotermes darwiniensis




SEQ ID NO: 142
146197251
uncultured symbiotic protist of
258
404




Neotermes koshunensis




SEQ ID NO: 143
146197319
uncultured symbiotic protist of
259
402




Mastotermes darwiniensis




SEQ ID NO: 144
146197071
uncultured symbiotic protist of
259
402




Reticulitermes speratus




SEQ ID NO: 145
146197075
uncultured symbiotic protist of
260
402




Reticulitermes speratus




SEQ ID NO: 146
146197159
uncultured symbiotic protist of
260
410




Hodotermopsis sjoestedti




SEQ ID NO: 147
146197405
uncultured symbiotic protist of
261
412




Cryptocercus punctulatus




SEQ ID NO: 148
146197327
uncultured symbiotic protist of
264
408




Mastotermes darwiniensis




SEQ ID NO: 149
146197261
uncultured symbiotic protist of
258
404




Neotermes koshunensis






















TABLE 3








Signal
Catalytic

Cellulose





sequence
Domain

Binding



Database

(SS) start
(CD) start
Linker start 
Domain



Accession

and end
and end
and end
(CBD) start


SEQ ID NO:
Number
Species of Origin
position
position
position
and end





















SEQ ID NO: 1
BD29555*
Unknown
1-25
26-455
456-493
494-529


SEQ ID NO: 2
340514556
Trichoderma reesei
1-17
18-444
445-479
480-514


SEQ ID NO: 3
51243029
Penicillium occitanis
1-25
26-455
456-493
494-529


SEQ ID NO: 4
7cel (PDB) &
Trichoderma reesei
N/A
 1-427
N/A
N/A


SEQ ID NO: 5
67516425
Aspergillus nidulans
1-23
24-457
458-490
491-526




FGSC A4






SEQ ID NO: 6
46107376
Gibberella zeae PH-1
1-17
18-448
449-476
477-512


SEQ ID NO: 7
70992391
Aspergillus
1-26
27-460
461-496
497-532




fumigatus Af293






SEQ ID NO: 8
121699984
Aspergillus clavatus
1-27
27-460
461-503
504-539




NRRL 1






SEQ ID NO: 9
1906845
Claviceps purpurea
1-19
20-449
N/A
N/A


SEQ ID NO: 10
1gpi (PDB) &
Phanerochaete
N/A
 1-424
N/A
N/A




chrysosporium






SEQ ID NO: 11
119468034
Neosartorya fischeri
1-17
18-447
N/A
N/A




NRRL 181






SEQ ID NO: 12
7804883
Leptosphaeria
1-17
18-434
N/A
N/A




maculans






SEQ ID NO: 13
85108032
Neurospora crassa
1-17
18-445
446-485
486-521




N150






SEQ ID NO: 14
169859458
Coprinopsis cinerea
1-18
19-454
N/A
N/A




okayama






SEQ ID NO: 15
154292161
Botryotinia
1-18
19-443
444-555
556-596




fuckeliana B05-10






SEQ ID NO: 16
169615761 #
Phaeosphaeria
1
 2-426
N/A
N/A




nodorum SN15






SEQ ID NO: 17
4883502
Humicola grisea
1-22
23-446
N/A
N/A


SEQ ID NO: 18
950686
Humicola grisea
1-18
19-449
450-489
490-525


SEQ ID NO: 19
124491660
Chaetomium
1-22
23-446
N/A
N/A




thermophilum






SEQ ID NO: 20
58045187
Chaetomium
1-18
19-449
450-494
495-530




thermophilum






SEQ ID NO: 21
169601100 #
Phaeosphaeria
1
 2-416
N/A
N/A




nodorum SN15






SEQ ID NO: 22
169870197
Coprinopsis cinerea
1-18
19-454
N/A
N/A




okayama






SEQ ID NO: 23
3913806
Agaricus bisporus
1-18
19-447
448-470
471-506


SEQ ID NO: 24
169611094
Phaeosphaeria
1-18
19-447
N/A
N/A




nodorum SN15






SEQ ID NO: 25
3131
Phanerochaete
1-19
20-443
N/A
N/A




chrysosporium






SEQ ID NO: 26
70991503
Aspergillus
1-17
18-447
N/A
N/A




fumigatus Af293






SEQ ID NO: 27
294196
Phanerochaete
1-18
19-442
443-480
481-516




chrysosporium






SEQ ID NO: 28
18997123
Thermoascus
1-17
18-451
N/A
N/A




aurantiacus






SEQ ID NO: 29
4204214
Humicola grisea var
1-22
23-446
N/A
N/A




thermoidea






SEQ ID NO: 30
34582632
Trichoderma viride
1-18
18-444
445-479
480-514




(also known as








Hypochrea rufa)






SEQ ID NO: 31
156712284
Thermoascus
1-17
18-451
N/A
N/A




aurantiacus






SEQ ID NO: 32
39977899
Magnaporthe grisea
1-17
18-447
N/A
N/A




(oryzae) 70-15






SEQ ID NO: 33
20986705
Talaromyces
1-18
19-449
N/A
N/A




emersonii






SEQ ID NO: 34
22138843
Aspergillus oryzae
1-17
18-447
N/A
N/A


SEQ ID NO: 35
55775695
Penicillium
1-25
26-459
460-494
495-529




chrysogenum






SEQ ID NO: 36
171676762
Podospora anserina
1-18
19-450
451-492
493-528


SEQ ID NO: 37
146350520
Pleurotus sp Florida
1-18
19-453
N/A
N/A


SEQ ID NO: 38
37732123
Gibberella zeae
1-17
18-448
449-476
477-512


SEQ ID NO: 39
156055188
Sclerotinia
1-18
19-443
444-546
547-586




sclerotiorum 1980






SEQ ID NO: 40
453224
Phanerochaete
1-18
19-442
443-474
475-510




chrysosporium






SEQ ID NO: 41
50402144
Trichoderma reesei
1-17
18-444
445-478
479-513


SEQ ID NO: 42
115397177
Aspergillus terreus
1-23
24-457
458-505
506-541




NIH2624






SEQ ID NO: 43
154312003
Botryotinia
1-17
18-449
450-480
481-516




fuckeliana B05-10






SEQ ID NO: 44
49333365
Volvariella volvacea
1-18
19-453
N/A
N/A


SEQ ID NO: 45
729650
Penicillium
1-25
26-456
457-502
503-537




janthinellum






SEQ ID NO: 46
146424871
Pleurotus sp Florida
1-18
19-451
452-487
488-523


SEQ ID NO: 47
67538012
Aspergillus nidulans
1-17
18-443
N/A
N/A




FGSC A4






SEQ ID NO: 48
62006162
Fusarium poae
1-17
18-448
449-475
476-511


SEQ ID NO: 49
146424873
Pleurotus sp Florida
1-18
19-451
452-487
488-523


SEQ ID NO: 50
295937
Trichoderma viride
1-17
18-444
445-478
479-513


SEQ ID NO: 51
6179889 #
Alternaria alternata
1
 2-419
N/A
N/A


SEQ ID NO: 52
119483864
Neosartorya fischeri
1-26
27-461
462-499
500-535




NRRL 181






SEQ ID NO: 53
85083281
Neurospora crassa
1-20
21-445
N/A
N/A




OR74A






SEQ ID NO: 54
3913803
Cryphonectria
1-18
19-449
N/A
N/A




parasitica






SEQ ID NO: 55
60729633
Corticium rolfsii
1-18
19-448
449-492
493-528


SEQ ID NO: 56
39971383
Magnaporthe grisea
1-17
18-443
N/A
N/A




70-15






SEQ ID NO: 57
39973029
Magnaporthe grisea
1-19
20-443
N/A
N/A




70-15






SEQ ID NO: 58
1170141
Fusarium
1-17
18-448
449-478
479-514




oxysporum






SEQ ID NO: 59
121710012
Aspergillus clavatus
1-17
18-447
N/A
N/A




NRRL 1






SEQ ID NO: 60
17902580
Penicillium
1-25
26-455
456-493
494-529




funiculosum






SEQ ID NO: 61
1346226
Humicola grisea var
1-18
19-449
450-489
490-525




thermoidea






SEQ ID NO: 62
156712282
Chaetomium
1-18
19-449
450-496
497-532




thermophilum






SEQ ID NO: 63
169768818
Aspergillus oryzae
1-25
26-460
N/A
N/A




RIB40






SEQ ID NO: 64
46241270
Gibberella pulicaris
1-17
18-448
449-474
475-510


SEQ ID NO: 65
49333363
Volvariella volvacea
1-18
19-451
452-476
477-512


SEQ ID NO: 66
46395332
Irpex lacteus
1-18
19-447
448-485
486-521


SEQ ID NO: 67
50844407 #
Chaetomium
N/A
 1-424
425-469
470-505




thermophilum var








thermophilum






SEQ ID NO: 68
4586347
Irpex lacteus
1-18
19-448
449-490
491-526


SEQ ID NO: 69
3980202
Phanerochaete
1-18
19-443
444-475
476-511




chrysosporium






SEQ ID NO: 70
27125837
Melanocarpus
1-23
23-447
N/A
N/A




albomyces






SEQ ID NO: 71
171696102
Podospora anserina
1-17
17-448
N/A
N/A


SEQ ID NO: 72
3913802
Cochliobolus
1-18
19-449
N/A
N/A




carbonum






SEQ ID NO: 73
50403723
Trichoderma viride
1-17
18-444
445-479
480-514


SEQ ID NO: 74
3913798
Aspergillus
1-22
23-458
459-505
506-540




aculeatus






SEQ ID NO: 75
66828465
Dictyostelium
1-19
20-452
N/A
N/A




discoideum






SEQ ID NO: 76
156060391
Sclerotinia
1-17
18-435
436-470
471-504




sclerotiorum 1980






SEQ ID NO: 77
116181754
Chaetomium
1-17
18-446
N/A
N/A




globosum CBS 148-








51






SEQ ID NO: 78
145230535
Aspergillus niger
1-21
22-457
458-500
501-536


SEQ ID NO: 79
46241266
Nectria
1-18
18-448
449-472
473-508




haematococca mpVI






SEQ ID NO: 80
1q9h (PDB) #
Talaromyces
N/A
 1-431
N/A
N/A




emersonii






SEQ ID NO: 81
157362170
Polyporus
1-18
19-453
N/A
N/A




arcularius






SEQ ID NO: 82
7804885
Leptosphaeria
1-20
21-440
N/A
N/A




maculans






SEQ ID NO: 83
121852
Phanerochaete
1-18
19-442
443-480
481-516




chrysosporium






SEQ ID NO: 84
126013214
Penicillium
1-17
18-448
N/A
N/A




decumbens






SEQ ID NO: 85
156048578
Sclerotinia
1-16
17-446
N/A
N/A




sclerotiorum 1980






SEQ ID NO: 86
156712278
Acremonium
1-17
18-447
448-487
488-523




thermophilum






SEQ ID NO: 87
21449327
Aspergillus nidulans
1-17
18-443
N/A
N/A


SEQ ID NO: 88
171683762
Podospora anserina
1-22
23-448
N/A
N/A


SEQ ID NO: 89 
56718412
Thermoascus
1-17
18-451
N/A
N/A




aurantiacus var








levisporus






SEQ ID NO: 90 
15824273
Pseudotrichonympha
1-20
21-447
N/A
N/A




grassii






SEQ ID NO: 91
115390801
Aspergillus terreus
1-17
18-444
N/A
N/A




NIH2624






SEQ ID NO: 92
453223
Phanerochaete
1-18
19-442
443-474
475-510




chrysosporium






SEQ ID NO: 93
3132
Phanerochaete
1-19
20-436
437-467
468-504




chrysosporium






SEQ ID NO: 94
16304152
Thermoascus
1-17
18-450
N/A
N/A




aurantiacus






SEQ ID NO: 95
156712280
Acremonium
1-21
22-453
N/A
N/A




thermophilum






SEQ ID NO: 96 
5231154
Volvariella volvacea
1-15
16-472
473-500
501-536


SEQ ID NO: 97 
116200349
Chaetomium
1-20
21-445
N/A
N/A




globosum CBS 148-








51






SEQ ID NO: 98 
4586343
Irpex lacteus
1-18
19-447
448-481
482-517


SEQ ID NO: 99 
15321718
Lentinula edodes
1-18
19-450
451-480
481-516


SEQ ID NO: 100
146424875
Pleurotus sp Florida
1-18
19-451
452-487
488-523


SEQ ID NO: 101
62006158
Fusarium venenatum
1-17
18-448
449-471
472-507


SEQ ID NO: 102
296027
Phanerochaete
1-18
19-442
443-480
481-516




chrysosporium






SEQ ID NO: 103
154449709
Fusicoccum sp
1-19
20-457
N/A
N/A




BCC4124






SEQ ID NO: 104
169859460
Coprinopsis cinerea
1-18
19-454
N/A
N/A




okayama






SEQ ID NO: 105
50400675
Trichoderma
1-17
18-440
441-470
471-505




harzianum






SEQ ID NO: 106
729649
Neurospora crassa
1-17
18-439
440-480
481-516


SEQ ID NO: 107
119472134
Neosartorya fischeri
1-26
27-460
461-494
495-530




NRRL 181






SEQ ID NO: 108
117935080
Chaetomium
1-22
23-446
N/A
N/A




thermophilum






SEQ ID NO: 109
154300584
Botryotinia
1-16
17-446
N/A
N/A




fuckeliana B05-10






SEQ ID NO: 110
15824271
Pseudotrichonympha
1-20
21-447
N/A
N/A




grassii






SEQ ID NO: 111
4586345
Irpex lacteus
1-18
19-447
448-487
488-523


SEQ ID NO: 112
46241268
Gibberella avenacea
1-17
18-449
450-478
478-513


SEQ ID NO: 113
6164684
Aspergillus niger
1-21
22-457
458-500
501-536


SEQ ID NO: 114
6164682
Aspergillus niger
1-17
18-445
N/A
N/A


SEQ ID NO: 115
33733371
Chrysosporium
1-17
18-448
449-490
491-526




lucknowense








US6573086-10






SEQ ID NO: 116
29160311
Thielavia
1-18
18-448
449-502
503-538




australiensis






SEQ ID NO: 117
146197087
uncultured symbiotic
1-22
23-435
N/A
N/A




protist of








Reticulitermes








speratus






SEQ ID NO: 118
146197237
uncultured symbiotic
1-20
21-442
N/A
N/A




protist of Neotermes








koshunensis






SEQ ID NO: 119
146197067
uncultured symbiotic
1-22
23-435
N/A
N/A




protist of








Reticulitermes








speratus






SEQ ID NO: 120
146197407
uncultured symbiotic
1-19
20-445
N/A
N/A




protist of








Cryptocercus








punctulatus






SEQ ID NO: 121 
146197157
uncultured symbiotic
1-20
21-443
N/A
N/A




protist of








Hodotermopsis








sjoestedti






SEQ ID NO: 122 
146197403
uncultured symbiotic
1-19
20-445
N/A
N/A




protist of








Cryptocercus








punctulatus






SEQ ID NO: 123
146197081
uncultured symbiotic
1-22
23-443
N/A
N/A




protist of








Reticulitermes








speratus






SEQ ID NO: 124
146197413
uncultured symbiotic
1-19
20-445
N/A
N/A




protist of








Cryptocercus








punctulatus






SEQ ID NO: 125
146197309
uncultured symbiotic
1-20 
21-435
N/A
N/A




protist of








Mastotermes








darwiniensis






SEQ ID NO: 126
146197227
uncultured symbiotic
1-19
20-437
N/A
N/A




protist of Neotermes








koshunensis






SEQ ID NO: 127
146197253
uncultured symbiotic
1-21
21-442
N/A
N/A




protist of Neotermes








koshunensis






SEQ ID NO: 128
146197099
uncultured symbiotic
1-22
23-434
N/A
N/A




protist of








Reticulitermes








speratus






SEQ ID NO: 129
146197409
uncultured symbiotic
1-19
20-444
N/A
N/A




protist of








Cryptocercus








punctulatus






SEQ ID NO: 130
146197315
uncultured symbiotic
1-20
21-435
N/A
N/A




protist of








Mastotermes








darwiniensis






SEQ ID NO: 131
146197411
uncultured symbiotic
1-19
20-445
N/A
N/A




protist of








Cryptocercus








punctulatus






SEQ ID NO: 132
146197161
uncultured symbiotic
1-20
21-446
N/A
N/A




protist of








Hodotermopsis








sjoestedti






SEQ ID NO: 133
146197323
uncultured symbiotic
1-20
21-435
N/A
N/A




protist of








Mastotermes








darwiniensis






SEQ ID NO: 134
146197077
uncultured symbiotic
1-21
22-448
N/A
N/A




protist of








Reticulitermes








speratus






SEQ ID NO: 135
146197089
uncultured symbiotic
1-22
23-433
N/A
N/A




protist of








Reticulitermes








speratus






SEQ ID NO: 136 
146197091
uncultured symbiotic
1-22
23-434
N/A
N/A




protist of








Reticulitermes








speratus






SEQ ID NO: 137
146197097
uncultured symbiotic
1-22
23-435
N/A
N/A




protist of








Reticulitermes








speratus






SEQ ID NO: 138 
146197095
uncultured symbiotic
1-22
23-435
N/A
N/A




protist of








Reticulitermes








speratus






SEQ ID NO: 139 
146197401
uncultured symbiotic
1-19
20-445
N/A
N/A




protist of








Cryptocercus








punctulatus






SEQ ID NO: 140 
146197225
uncultured symbiotic
1-19
20-437
N/A
N/A




protist of Neotermes








koshunensis






SEQ ID NO: 141 
146197317
uncultured symbiotic
1-20
21-435
N/A
N/A




protist of








Mastotermes








darwiniensis






SEQ ID NO: 142
146197251
uncultured symbiotic
1-19
20-437
N/A
N/A




protist of Neotermes








koshunensis






SEQ ID NO: 143 
146197319
uncultured symbiotic
1-20
21-435
N/A
N/A




protist of








Mastotermes








darwiniensis






SEQ ID NO: 144
146197071
uncultured symbiotic
1-25
26-435
N/A
N/A




protist of








Reticulitermes








speratus






SEQ ID NO: 145 
146197075
uncultured symbiotic
1-22
23-435
N/A
N/A




protist of








Reticulitermes








speratus






SEQ ID NO: 146
146197159
uncultured symbiotic
1-23
24-443
N/A
N/A




protist of








Hodotermopsis








sjoestedti






SEQ ID NO: 147 
146197405
uncultured symbiotic
1-19
20-445
N/A
N/A




protist of








Cryptocercus








punctulatus






SEQ ID NO: 148 
146197327
uncultured symbiotic
1-20
21-441
N/A
N/A




protist of








Mastotermes








darwiniensis






SEQ ID NO: 149 
146197261
uncultured symbiotic
1-19
20-437
N/A
N/A




protist of Neotermes








koshunensis






















TABLE 4









Amino acid
Amino acid
Position of






positions of
positions of
catalytic


Sequence
Database


fragment in
active site loop
residues in


Identifier
Accession

Amino acid sequence of fragment of catalytic domain
sequence
in sequence
sequence


(SEQ ID NO:)
Number
Species of Origin
including loop and catalytic residue
identifer
identifer
identifier







SEQ ID NO: 150
BD29555*
Unknown
NVEGWTPSSNNANTGLGNHGACCAELDIWEANS
210-242
214-226
234, 239


 


SEQ ID NO: 151
340514556
Trichoderma
NVEGWTPSANNANTGIGNHGACCAELDIWEANS
205-237
209-221
229, 234




reesei


 


SEQ ID NO: 152
51243029
Penicillium
NVEGWEPSSNNANTGIGGHGSCCSEMDIWEANS
210-242
214-226
234, 239




occitanis


 


SEQ ID NO: 153
7cel (PDB) &
Trichoderma
NVEGWEPSSNNANTGIGGHGSCCSEMDIWQANS
188-220
192-204
212, 217




reesei


 


SEQ ID NO: 154
67516425
Aspergillus
NVEGWESSDTNPNGGVGNHGSCCAEMDIWEANS
211-243
215-227
235, 240




nidulans FGSC




A4


 


SEQ ID NO: 155
46107376
Gibberella zeae
NSDGWQPSDSDVNGGIGNLGTCCPEMDIWEANS
205-237
209-221
229, 234




PH-1


 


SEQ ID NO: 156
70992391
Aspergillus
NVEGWQPSSNDANAGTGNHGSCCAEMDIWEANS
214-246
218-230
238, 243




fumigatus Af293


 


SEQ ID NO: 157
121699984
Aspergillus
NVEGWTPSSSDANAGNGGHGSCCAEMDIWEANS
214-246
218-230
238, 243




clavatus NRRL 1


 


SEQ ID NO: 158
1906845
Claviceps
NSKDWIPSKSDANAGIGSLGACCREMDIWEANN
206-238
210-222
230,235




purpurea


 


SEQ ID NO: 159
lgpi (PDB) &
Phanerochaete
NVGNWTETG--SNTGTGSYGTCCSEMDIWEANN
185-215
189-199
207, 212




chrysosporium


 


SEQ ID NO: 160
119468034
Neosartorya
NVEGWKPSSNDKNAGVGGHGSCCPEMDIWEANS
202-234
206-218
226, 231




fischeri NRRL




181


 


SEQ ID NO: 161
7804883
Leptosphaeria
NVEGWQPSKNDQNAGVGGHGSCCAEMDIWEANS
193-225
197-209
217, 222




maculans


 


SEQ ID NO: 162
85108032
Neurospora crassa
NVEGWTPSTNDANAGIGDHGTCCSEMDIWEANK
205-237
209-221
229, 234




N150 (OR74A)


 


SEQ ID NO: 163
169859458
Coprinopsis
NSADWTPSETDPNAGRGRYGICCAEMDIWEANS
207-239
211-223
231, 236




cinerea okayama


 


SEQ ID NO: 164
154292161
Botryotinia
NVEGWVPDSNSANSGTGNIGSCCSEFDVWEANS
203-235
207-219
227, 232




fuckeliana B05-10


 


SEQ ID NO: 165
169615761 #
Phaeosphaeria
NADGWQASTSDPNAGVGKKGACCAEMDVWEANS
183-215
187-199
207, 212




nodorum SN15


 


SEQ ID NO: 166
4883502
Humicola grisea
NIEGWRPSTNDPNAGVGPMGACCAEIDVWESNA
208-240
212-224
232, 237


 


SEQ ID NO: 167
950686
Humicola grisea
NIEGWTGSTNDPNAGAGRYGTCCSEMDIWEANN
207-239
211-223
231, 236


 


SEQ ID NO: 168
124491660
Chaetomium
NIEGWRPSTNDANAGVGPYGACCAEIDVWESNA
209-241
213-225
233, 238




thermophilum


 


SEQ ID NO: 169
58045187
Chaetomium
NIENWTPSTNDANAGFGRYGSCCSEMDIWEANN
207-239
211-223
231, 236




thermophilum


 


SEQ ID NO: 170
169601100 #
Phaeosphaeria
NVEGWKPSDNDANAGVGGHGSCCAEMDIWEANS
174-206
178-190
198, 203




nodorum SN15


 


SEQ ID NO: 171
169870197
Coprinopsis
NSVGWEPSETDSNAGRGRYGICCAEMDIWEANS
207-239
211-223
231, 236




cinerea okayama


 


SEQ ID NO: 172
3913806
Agaricus bisporus
NSEGWEGSPNDVNAGTGNFGACCGEMDIWEANS
203-235
207-219
227, 232


 


SEQ ID NO: 173
169611094
Phaeosphaeria
NVEGWNPSDADPNAGSGKIGACCPEMDIWEANS
208-240
212-224
232, 237




nodorum SN15


 


SEQ ID NO: 174
3131
Phanerochaete
NVQGWNATS--ATTGTGSYGSCCTELDIWEANS
204-234
208-218
226, 231




chrysosporium


 


SEQ ID NO: 175
70991503
Aspergillus
NVEGWEPSSSDKNAGVGGHGSCCPEMDIWEANS
202-234
206-218
226, 231




fumigatus Af293


 


SEQ ID NO: 176
294196
Phanerochaete
NVEGWNATS--ANAGTGNYGTCCTEMDIWEANN
203-233
207-217
225, 230




chrysosporium


 


SEQ ID NO: 177
18997123
Thermoascus
NVEGWQPSANDPNAGVGNHGSSCAEMDVWEANS
205-237
209-221
229, 234




aurantiacus


 


SEQ ID NO: 178
4204214
Humicola grisea
NIEGWRPSTNDPNAGVGPMGACCAEIDVWESNA
208-240
212-224
232, 237




var thermoidea


 


SEQ ID NO: 179
34582632
Trichoderma
NVEGWEPSSNNANTGIGGHGSCCSEMDIWEANS
205-237
209-221
229, 234




viride (also known




as Hypochrea




rufa)


 


SEQ ID NO: 180
156712284
Thermoascus
NVEGWQPSANDPNAGVGNHGSCCAEMDVWEANS
205-237
209-221
229, 234




aurantiacus


 


SEQ ID NO: 181
39977899
Magnaporthe
NVEGWQPSSGDANSGVGNMGSCCAEMDIWEANS
205-237
209-221
229, 234




grisea (oryzae)




70-15


 


SEQ ID NO: 182
20986705
Talaromyces
NVEGWQPSSNNANTGIGDHGSCCAEMDVWEANS
203-235
207-219
227, 232




emersonii


 


SEQ ID NO: 183
22138843
Aspergillus oryzae
R-KGWEPSDSDKNAGVGGHGSCCPQMDIWEANS
203-234
206-218
226, 231


 


SEQ ID NO: 184
55775695
Penicillium
NVEGWEPSSSDVNGGTGNYGSCCAEMDIWEANS
213-245
217-229
237, 242




chrysogenum


 


SEQ ID NO: 185
171676762
Podospora
NIEGWNPSTNDVNAGAGRYGTCCSEMDIWEANN
207-239
211-223
231, 236




anserina


 


SEQ ID NO: 186
146350520
Pleurotus sp
NVQGWQPSPNDSNAGKGQYGSCCAEMDIWEANS
207-239
211-223
231, 236




Florida


 


SEQ ID NO: 187
37732123
Gibberella zeae
NSDGWQPSDSDVNGGIGNLGTCCPEMDIWEANS
205-237
209-221
229, 234


 


SEQ ID NO: 188
156055188
Sclerotinia
NNEGWVPDSNSANSGTGNIGSCCSEFDVWEANS
203-235
207-219
227, 232




sclerotiorum 1980


 


SEQ ID NO: 189
453224
Phanerochaete
NVGNWTETG--SNTGTGSYGTCCSEMDIWEANN
203-233
207-217
225, 230




chrysosporium


 


SEQ ID NO: 190
50402144
Trichoderma
NVEGWEPSSNNANTGIGGHGSCCSEMDIWEANS
205-237
209-221
229, 234




reesei


 


SEQ ID NO: 191
115397177
Aspergillus terreus
NVEGWEPSANDANAGTGNHGSCCAEMDIWEANS
211-243
215-227
235, 240




NIH2624


 


SEQ ID NO: 192
154312003
Botryotinia
NSVGWTPSSNDVNAGAGQYGSCCSEMDIWEANK
206-238
210-222
230, 235




fuckeliana B05-10


 


SEQ ID NO: 193
49333365
Volvariella
NVQGWQPSPNDTNAGTGNYGACCNEMDVWEANS
207-239
211-223
231, 236




volvacea


 


SEQ ID NO: 194
729650
Penicillium
NVDGWTPSKNDVNSGIGNHGSCCAEMDIWEANS
211-243
215-227
235, 240




janthinellum


 


SEQ ID NO: 195
146424871
Pleurotus sp
NILDWSASATDANAGNGRYGACCAEMDIWEANS
206-238
210-222
230, 235




Florida


 


SEQ ID NO: 196
67538012
Aspergillus
NVEGWEPSDSDANAGVGGMGTCCPEMDIWEANS
202-234
206-218
226, 231




nidulans FGSC




A4


 


SEQ ID NO: 197
62006162
Fusarium poae
NSDGWEPSKSDVNGGIGNLGTCCPEMDIWEANS
205-237
209-221
229, 234


 


SEQ ID NO: 198
146424873
Pleurotus sp
NILDWSGSATDPNAGNGRYGACCAEMDIWEANS
206-238
210-222
230, 235




Florida


 


SEQ ID NO: 199
295937
Trichoderma
NVEGWEPSSNNANTGIGGHGSCCSEMDIWEANS
205-237
209-221
229, 234




viride


 


SEQ ID NO: 200
6179889 #
Alternaria
NVEGWKPSSNDANAGVGGHGSCCAEMDIWEANS
177-209
181-193
201, 206




alternata


 


SEQ ID NO: 201
119483864
Neosartorya
NVEGWTPSSNNENTGLGNYGSCCAELDIWESNS
215-247
219-231
239, 244




fischeri NRRL




181


 


SEQ ID NO: 202
85083281
Neurospora crassa
NIEGWTPSTNDANAGVGPYGGCCAEIDVWESNA
207-239
211-223
231, 236




OR74A


 


SEQ ID NO: 203
3913803
Cryphonectria
NVEGWTPSTNDANAGVGGLGSCCSEMDVWEANS
206-238
210-222
230, 235




parasitica


 


SEQ ID NO: 204
60729633
Corticium rolfsii
NLLDWNATS--ANSGTGSYGSCCPEMDIWEANK
206-236
210-220
228, 233


 


SEQ ID NO: 205
39971383
Magnaporthe
NIEGWQPSSTDSSAGIGAQGACCAEIDIWESNK
205-237
209-221
229, 234




grisea 70-15


 


SEQ ID NO: 206
39973029
Magnaporthe
NIEGWKPSSNDANAGVGPYGACCAEIDVWESNA
206-238
210-222
230, 235




grisea 70-15


 


SEQ ID NO: 207
1170141
Fusarium
NSEGWKPSDSDVNAGVGNLGTCCPEMDIWEANS
205-237
209-221
229, 234




oxysporum


 


SEQ ID NO: 208
121710012
Aspergillus
NVEGWKPSDNDKNAGVGGYGSCCPEMDIWEANS
202-234
206-218
226, 231




clavatus NRRL 1


 


SEQ ID NO: 209
17902580
Penicillium
NVEGWTPSTNNSNTGIGNHGSCCAELDIWEANS
210-242
214-226
234, 239




funiculosum


 


SEQ ID NO: 210
1346226
Humicola grisea
NIEGWTGSTNDPNAGAGRYGTCCSEMDIWEANN
207-239
211-223
231,236




var thermoidea


 


SEQ ID NO: 211
156712282
Chaetomium
NVGNWTPSTNDANAGFGRYGSCCSEMDVWEANN
207-239
211-223
231, 236




thermophilum


 


SEQ ID NO: 212
169768818
Aspergillus oryzae
NVEGWVSSTNNANTGTGNHGSCCAELDIWESNS
214-246
218-230
238, 243




RIB40


 


SEQ ID NO: 213
46241270
Gibberella
NSDGWQPSKSDVNAGIGNMGTCCPEMDIWEANS
205-237
209-221
229, 234




pulicaris


 


SEQ ID NO: 214
49333363
Volvariella
NVAGWNGSPNDTNAGTGNWGACCNEMDIWEANS
205-237
209-221
229, 234




volvacea


 


SEQ ID NO: 215
46395332
Irpex lacteus
NVAGWTGSSSDPNSGTGNYGTCCSEMDIWEANS
202-234
206-218
226, 231


 


SEQ ID NO: 216
50844407 #
Chaetomium
NIENWTPSTNDANAGFGRYGSCCSEMDIWEANN
182-214
186-198
206, 211




thermophilum var




thermophilum


 


SEQ ID NO: 217
4586347
Irpex lacteus
NIVDWTASAGDANSGTGSFGTCCQEMDIWEANS
203-235
207-219
227, 232


 


SEQ ID NO: 218
3980202
Phanerochaete
NVGNWTETG--SNTGTGSYGTCCSEMDIWEANN
203-233
207-217
225, 230




chrysosporium


 


SEQ ID NO: 219
27125837
Melanocarpus
NIEGWKSSTSDPNAGVGPYGSCCAEIDVWESNA
210-242
214-226
234, 239




albomyces


 


SEQ ID NO: 220
171696102
Podospora
NVEGWGGAD--GNSGTGKYGICCAEMDIWEANS
206-236
210-220
228, 233




anserina


 


SEQ ID NO: 221
3913802
Cochliobolus
NVEGWNPSDADPNGGAGKIGACCPEMDIWEANS
208-240
212-224
232, 237




carbonum


 


SEQ ID NO: 222
50403723
Trichoderma
NVEGWEPSSNNANTGIGGHGSCCSEMDIWEANS
205-237
209-221
229, 234




viride


 


SEQ ID NO: 223
3913798
Aspergillus
NIEGWEPSSTDVNAGTGNHGSCCPEMDIWEANS
210-242
214-226
234, 239




aculeatus


 


SEQ ID NO: 224
66828465
Dictyostelium
NVDGWIPSTNNPNTGYGNLGSCCAEMDLWEANN
206-238
210-222
230, 235




discoideum


 


SEQ ID NO: 225
156060391
Sclerotinia
NSVGWTPSSNDVNTGTGQYGSCCSEMDIWEANK
192-224
196-208
216, 221




sclerotiorum 1980


 


SEQ ID NO: 226
116181754
Chaetomium
NSEGWGGED--GNSGTGKYGTCCAEMDIWEANL
203-233
207-217
225, 230




globosum CBS




148-51


 


SEQ ID NO: 227
145230535
Aspergillus niger
NCDGWEPSSNNVNTGVGDHGSCCAEMDVWEANS
209-241
213-225
233, 238


 


SEQ ID NO: 228
46241266
Nectria
NSDEWKPSDSDKNAGVGKYGTCCPEMDIWEANK
205-237
209-221
229, 234




haematococca




mpVI


 


SEQ ID NO: 229
1q9h (PDB) #
Talaromyces
NVEGWQPSSNNANTGIGDHGSCCAEMDVWEANS
185-217
189-201
209, 214




emersonii


 


SEQ ID NO: 230
157362170
Polyporus
NVLDWAGSSNDPNAGTGHYGTCCNEMDIWEANS
208-240
212-224
232, 237




arcularius


 


SEQ ID NO: 231
7804885
Leptosphaeria
NAEGWTKSASDPNSGVGKKGACCAQMDVWEANS
204-236
208-220
228, 233




maculans


 


SEQ ID NO: 232
121852
Phanerochaete
NVEGWNATS--ANAGTGNYGTCCTEMDIWEANN
203-233
207-217
225, 230




chrysosporium


 


SEQ ID NO: 233
126013214
Penicillium
NVEGWKPSANDKNAGVGPHGSCCAEMDIWEANS
201-233
205-217
225, 230




decumbens


 


SEQ ID NO: 234
156048578
Sclerotinia
NVDGWVPSSNNPNTGVGNYGSCCAEMDIWEANS
202-234
206-218
226, 231




sclerotiorum 1980


 


SEQ ID NO: 235
156712278
Acremonium
NIDGWQPSSNDANAGLGNHGSCCSEMDIWEANK
206-238
210-222
230, 235




thermophilum


 


SEQ ID NO: 236
21449327
Aspergillus
NVEGWEPSDSDANAGVGGMGTCCPEMDIWEANS
202-234
206-218
226, 231




nidulans (also




known as




Emericella




nidulans)


 


SEQ ID NO: 237
171683762
Podospora
NIEGWRESSNDENAGVGPYGGCCAEIDVWESNA
211-243
215-227
235, 240




anserine (S mat+)


 


SEQ ID NO: 238
56718412
Thermoascus
NVEGWQPSANDPNAGVGNHGSCCAEMDVWEANS
205-237
209-221
229, 234




aurantiacus var




levisporus


 


SEQ ID NO: 239
15824273
Pseudotrichonymp
NVENWKPQTNDENAGNGRYGACCTEMDIWEANK
200-232
204-216
224, 229




ha grassii


 


SEQ ID NO: 240
115390801
Aspergillus terreus
NVEGWTPSDNDKNAGVGGHGSCCPELDIWEANS
203-235
207-219
227, 232




NIH2624


 


SEQ ID NO: 241
453223
Phanerochaete
NVGNWTETG--SNTGTGSYGTCCSEMDIWEANN
203-233
207-217
225, 230




chrysosporium


 


SEQ ID NO: 242
3132
Phanerochaete
NVEGWLGTT--ATTGTGFFGSCCTDIALWEAND
202-232
206-216
224, 229




chrysosporium


 


SEQ ID NO: 243
16304152
Thermoascus
NVEGWQPSANDPNAGVGNHGSSCAEMDVWEANS
205-237
209-221
229, 234




aurantiacus


 


SEQ ID NO: 244
156712280
Acremonium
NSASWQPSSNDQNAGVGGMGSCCAEMDIWEANS
210-242
214-226
234, 239




thermophilum


 


SEQ ID NO: 245
5231154
Volvariella
NVQGWQPSPNDTNAGTGNYGACCNKMDVWEANS
220-252
224-236
244, 249




volvacea


 


SEQ ID NO: 246
116200349
Chaetomium
NYDGWTPSSNDANAGVGALGGCCAEIDVWESNA
207-239
211-223
231, 236




globosum CBS




148-51


 


SEQ ID NO: 247
4586343
Irpex lacteus
NVAGWAGSASDPNAGSGTLGTCCSEMDIWEANN
202-234
206-218
226, 231


 


SEQ ID NO: 248
15321718
Lentinula edodes
NVEGWTPSSTSPNAGTGGTGICCNEMDIWEANS
208-240
212-224
232, 237


 


SEQ ID NO: 249
146424875
Pleurotus sp
NVLDWSASATDDNAGNGRYGACCAEMDIWEANS
206-238
210-222
230, 235




Florida


 


SEQ ID NO: 250
62006158
Fusarium
NSDGWQPSKSDVNGGIGNLGTCCPEMDIWEANS
205-237
209-221
229, 234




venenatum


 


SEQ ID NO: 251
296027
Phanerochaete
NVEGWNATS--ANAGTGNYGTCCTEMDIWEANN
203-233
207-217
225, 230




chrysosporium


 


SEQ ID NO: 252
154449709
Fusicoccum sp
NVQNWTASSTDKNAGTGHYGSCCNEMDIWEANS
209-241
213-225
233, 238




BCC4124


 


SEQ ID NO: 253
169859460
Coprinopsis
NSVGWEPSETDPNAGKGQYGICCAEMDIWEANS
207-239
211-223
231, 236




cinerea okayama


 


SEQ ID NO: 254
50400675
Trichoderma
NVEGWEPSSNNANTGVGGHGSCCSEMDIWEANS
201-233
205-217
225, 230




harzianum




(anamorph of




Hypocrea lixii)


 


SEQ ID NO: 255
729649
Neurospora crassa
NVEGWTPSTNDAN-GIGDHGSCCSEMDIWEANK
200-231
204-215
223, 228




(OR74A)


 


SEQ ID NO: 256
119472134
Neosartorya
NVEGWQPSSNDANAGTGNHGSCCAEMDIWEANS
214-246
218-230
238, 243




fischeri NRRL




181


 


SEQ ID NO: 257
117935080
Chaetomium
NIEGWRPSTNDANAGVGPYGACCAEIDVWESNA
209-241
213-225
233, 238




thermophilum


 


SEQ ID NO: 258
154300584
Botryotinia
NVDGWVPSSNNANTGVGNHGSCCAEMDIWEANS
202-234
206-218
226, 231




fuckeliana B05-10


 


SEQ ID NO: 259
15824271
Pseudotrichonymp
NVENWKPQTNDENAGNGRYGACCTEMDIWEANK
200-232
204-216
224, 229




ha grassii


 


SEQ ID NO: 260
4586345
Irpex lacteus
NVEGWTGSSTDSNSGTGNYGTCCSEMDIWEANS
202-234
206-218
226, 231


 


SEQ ID NO: 261
46241268
Gibberella
NSDGWKPSDSDINAGIGNMGTCCPEMDIWEANS
205-237
209-221
229, 234




avenacea


 


SEQ ID NO: 262
6164684
Aspergillus niger
NCDGWEPSSNNVNTGVGDHGSCCAEMDVWEANS
209-241
213-225
233, 238


 


SEQ ID NO: 263
6164682
Aspergillus niger
NVDGWEPSSNNDNTGIGNHGSCCPEMDIWEANK
203-235
207-219
227, 232


 


SEQ ID NO: 264
33733371
Chrysosporium
NVENWQSSTNDANAGTGKYGSCCSEMDVWEANN
206-238
210-222
230, 235




luckowense




US6573086-10


 


SEQ ID NO: 265
29160311
Thielavia
NVEGWESSTNDANAGSGKYGSCCTEMDVWEANN
206-238
210-222
230, 235




australiensis


 


SEQ ID NO: 266
146197087
uncultured
NVDDWKPQDNDENSGNGKLGTCCSEMDIWEGNM
197-229
201-213
221, 226




symbiotic protist




of Reticuliternies




speratus


 


SEQ ID NO: 267
146197237
uncultured
NSEGWKPQSGDKNAGNGKYGSCCSEMDVWESNS
200-232
204-216
224, 229




symbiotic protist




of Neotermes




koshunensis


 


SEQ ID NO: 268
146197067
uncultured
NVDDWKPQDNDENSGNGKLGTCCSEMDIWEGNM
197-229
201-213
221, 226




symbiotic protist




of Reticulitermes




speratus


 


SEQ ID NO: 269
146197407
uncultured
NVLDWKPQSNDENSGNGRYGACCTEMDIWEANS
198-230
202-214
222, 227




symbiotic protist




of Cryptocercus




punctulatus


 


SEQ ID NO: 270
146197157
uncultured
NVEGWKPSDNDENAGTGKWGACCTEMDIWEANK
201-233
205-217
225, 230




symbiotic protist




of Hodotermopsis




sjoestedti


 


SEQ ID NO: 271
146197403
uncultured
NVLDWKPQSNDENSGNGRYGACCTEMDIWEANS
198-230
202-214
222, 227




symbiotic protist




of Cryptocercus




punctulatus


 


SEQ ID NO: 272
146197081
uncultured
NVDDWKPQDNDENSGDGKLGTCCSEMDIWEGNA
197-229
201-213
221, 226




symbiotic protist




of Reticulitermes




speratus


 


SEQ ID NO: 273
146197413
uncultured
NVLDWKPQSNDENSGNGRYGACCTEMDIWEANS
198-230
202-214
222, 227




symbiotic protist




of Cryptocercus




punctulatus


 


SEQ ID NO: 274
146197309
uncultured
NSDGWKPQSNDKNSGNGKYGSCCSEMDIWEANS
196-228
200-212
220, 225




symbiotic protist




of Mastotermes




darwiniensis


 


SEQ ID NO: 275
146197227
uncultured
NSDGWKPQKNDKNSGNGKYGSCCSEMDIWEANS
195-227
199-211
219, 224




symbiotic protist




of Neotermes




koshunensis


 


SEQ ID NO: 276
146197253
uncultured
NSEGWKPQSGDKNAGNGKYGSCCSEMDVWESNS
200-232
204-216
224, 229




symbiotic protist




of Neotermes




koshunensis


 


SEQ ID NO: 277
146197099
uncultured
NVLDWKPQSNDENAGTGRYGTCCTEMDIWEANS
197-229
201-213
221, 226




symbiotic protist




of Reticulitermes




speratus


 


SEQ ID NO: 278
146197409
uncultured
NVLDWKPQSNDENSGNGRWGARCTEMDIWEANS
198-230
202-214
222, 227




symbiotic protist




of Cryptocercus




punctulatus


 


SEQ ID NO: 279
146197315
uncultured
NSDGWKPQSNDKNSGNGKYGSCCSEMDIWEANS
196-228
200-212
220, 225




symbiotic protist




of Mastotermes




darwiniensis


 


SEQ ID NO: 280
146197411
uncultured
NVLDWKPQSNDENSGNGRYGACCTEMDIWEANS
198-230
202-214
222, 227




symbiotic protist




of Cryptocercus




punctulatus


 


SEQ ID NO: 281
146197161
uncultured
NVQDWKPSDNDDNAGTGHYGACCTEMDIWEANK
201-233
205-217
225, 230




symbiotic protist




of Hodotermopsis




sjoestedti


 


SEQ ID NO: 282
146197323
uncultured
NSDGWKPQSNDKNSGNGKYGSCCSEMDIWEANS
196-228
200-212
220, 225




symbiotic protist




of Mastotermes




darwiniensis


 


SEQ ID NO: 283
146197077
uncultured
NVLDWKPQETDENSGNGRYGTCCTEMDIWEANS
201-233
205-217
225, 230




symbiotic protist




of Reticulitermes




speratus


 


SEQ ID NO: 284
146197089
uncultured
NVEDWKPQDNDENSGNGKLGTCCSEMDIWEGNA
197-229
201-213
221, 226




symbiotic protist




of Reticulitermes




speratus


 


SEQ ID NO: 285
146197091
uncultured
NVLDWKPQSNDENAGTGRYGTCCTEMDIWEANS
197-229
201-213
221, 226




symbiotic protist




of Reticulitermes




speratus


 


SEQ ID NO: 286
146197097
uncultured
NVDDWKPQDNDENSGNGKLGTCCSEMDIWEGNA
197-229
201-213
221, 226




symbiotic protist




of Reticulitermes




speratus


 


SEQ ID NO: 287
146197095
uncultured
NVDDWKPQDNDENSGNGKLGTCCSEMDIWEGNA
197-229
201-213
221, 226




symbiotic protist




of Reticulitermes




speratus


 


SEQ ID NO: 288
146197401
uncultured
NVLDWKPQSNDENSGNGRYGACCIEMDIWEANS
198-230
202-214
222, 227




symbiotic protist




of Cryptocercus




punctulatus


 


SEQ ID NO: 289
146197225
uncultured
NSDGWKPQKNDKNSGNGKYGSCCSEMDIWEANS
195-227
199-211
219, 224




symbiotic protist




of Neotermes




koshunensis


 


SEQ ID NO: 290
146197317
uncultured
NSDGWKPQSNDKNSGNGKYGSCCSEMDIWEANS
196-228
200-212
220, 225




symbiotic protist




of Mastotermes




darwiniensis


 


SEQ ID NO: 291
146197251
uncultured
NSDGWKPQKNDKNSGNGRYGSCCSEMDVWEANS
195-227
199-211
219, 224




symbiotic protist




of Neotermes




koshunensis


 


SEQ ID NO: 292
146197319
uncultured
NSDGWKPQSNDKNSGNGKYGSCCSEMDIWEANS
196-228
200-212
220, 225




symbiotic protist




of Mastotermes




darwiniensis


 


SEQ ID NO: 293
146197071
uncultured
NILDWKPSSNDENAGAGRYGTCCTEMDIWEANS
200-232
204-216
224, 229




symbiotic protist




of Reticulitermes




speratus


 


SEQ ID NO: 294
146197075
uncultured
NVDDWKPQDNDENSGNGKLGTCCSEMDIWEGNA
197-229
201-213
221, 226




symbiotic protist




of Reticulitermes




speratus


 


SEQ ID NO: 295
146197159
uncultured
NVKDWKPQETDENAGNGHYGACCTEMDIWEANS
197-229
201-213
221, 226




symbiotic protist




of Hodotermopsis




sjoestedti


 


SEQ ID NO: 296
146197405
uncultured
NVLDWKPQSNDENSGNGRYGACCTEMDIWEANS
198-230
202-214
222, 227




symbiotic protist




of Cryptocercus




punctulatus


 


SEQ ID NO: 297
146197327
uncultured
NSDGWKPQDNDENSGNGKYGSCCSEMDIWEANS
201-233
205-217
225, 230




symbiotic protist




of Mastotermes




darwiniensis


 


SEQ ID NO: 298
146197261
uncultured
NSDGWKPQKNDKNSGNGKYGSCCSEMDIWEANS
195-227
199-211
219, 224




symbiotic protist




of Neotermes




koshunensis


















TABLE 5






Tolerance to
Tolerance to



250 mg/L 
cellobiose 



cellobiose
accumulation



% Activity 
% Activity 



in 4-MUL Assay
in Bagasse Assay


Substitution(s)
(+/−Cellobiose)±
(−/+BG)¥

















None
25%
60%


R273K/R422K
95%
84%


R273K/Y274Q/D281K/Y410H/
78%
ND


P411G/R422K




















TABLE 6








Tolerance to
Tolerance to




250 mg/L cellobiose
cellobiose accumulation




% Activity 
% Activity 




in 4-MUL Assay
in Bagasse Assay



Substitution(s)
(+/−Cellobiose)±
(−/+BG)¥




















None
23%
74%



R268K/R411K
92%
94%



R268A/R411A
92%
95%



R268A/R411K
97%
94%



R268K/R411A
97%
102% 



R268K
ND
92%



R268A
ND
86%



R411K
ND
89%



R411A
ND
94%


















TABLE 7





SEQ ID NO.
Amino acid sequence







SEQ ID NO: 1
MSALNSFNMY KSALILGSLL ATAGAQQIGT YTAETHPSLS WSTCKSGGSC TTNSGAITLD ANWRWVHGVN TSTNCYTGNT WNTAICDTDA



SCAQDCALDG ADYSGTYGIT TSGNSLRLNF VTGSNVGSRT YLMADNTHYQ IFDLLNQEFT FTVDVSHLPC GLNGALYFVT MDADGGVSKY



PNNKAGAQYG VGYCDSQCPR DLKFIAGQAN VEGWTPSSNN ANTGLGNHGA CCAELDIWEA NSISEALTPH PCDTPGLSVC TTDACGGTYS



SDRYAGTCDP DGCDFNPYRL GVTDFYGSGK TVDTTKPITV VTQFVTDDGT STGTLSEIRR YYVQNGVVIP QPSSKISGVS GNVINSDFCD



AEISTFGETA SFSKHGGLAK MGAGMEAGMV LVMSLWDDYS VNMLWLDSTY PTNATGTPGA ARGSCPTTSG DPKTVESQSG SSYVTFSDIR VGPFNSTFSG GSSTGGSSTT



TASGTTTTKA SSTSTSSTST GTGVAAHWGQ CGGQGWTGPT TCASGTTCTV VNPYYSQCL


 


SEQ ID NO: 2
MYRKLAVISA FLATARAQSA CTLQSETHPP LTWQKCSSGG TCTQQTGSVV IDANWRWTHA TNSSTNCYDG NTWSSTLCPD NETCAKNCCL



DGAAYASTYG VTTSGNSLSI GFVTQSAQKN VGARLYLMAS DTTYQEFTLL GNEFSFDVDV SQLPCGLNGA LYFVSMDADG GVSKYPTNTA



GAKYGTGYCD SQCPRDLKFI NGQANVEGWE PSSNNANTGI GGHGSCCSEM DIWEANSISE ALTPHPCTTV GQEICEGDGC GGTYSDNRYG



GTCDPDGCDW NPYRLGNTSF YGPGSSFTLD TTKKLTVVTQ FETSGAINRY YVQNGVTFQQ PNAELGSYSG NELNDDYCTA EEAEFGGSSF



SDKGGLTQFK KATSGGMVLV MSLWDDYYAN MLWLDSTYPT NETSSTPGAV RGSCSTSSGV PAQVESQSPN AKVTFSNIKF GPIGSTGNPS



GGNPPGGNPP GTTTTRRPAT TTGSSPGPTQ SHYGQCGGIG YSGPTVCASG TTCQVLNPYY SQCL


 


SEQ ID NO: 3
MSALNSFNMY KSALILGSLL ATAGAQQIGT YTAETHPSLS WSTCKSGGSC TTNSGAITLD ANWRWVHGVN TSTNCYTGNT WNSAICDTDA



SCAQDCALDG ADYSGTYGIT TSGNSLRLNF VTGSNVGSRT YLMADNTHYQ IFDLLNQEFT FTVDVSHLPC GLNGALYFVT MDADGGVSKY



PNNKAGAQYG VGYCDSQCPR DLKFIAGQAN VEGWTPSANN ANTGIGNHGA CCAELDIWEA NSISEALTPH PCDTPGLSVC TTDACGGTYS



SDRYAGTCDP DGCDFNPYRL GVTDFYGSGK TVDTTKPFTV VTQFVTNDGT STGSLSEIRR YYVQNGVVIP QPSSKISGIS GNVINSDYCA



AEISTFGGTA SFNKHGGLTN MAAGMEAGMV LVMSLWDDYA VNMLWLDSTY PTNATGTPGA ARGTCATTSG DPKTVESQSG SSYVTFSDIR VGPFNSTFSG GSSTGGSTTT



TASRTTTTSA SSTSTSSTST GTGVAGHWGQ CGGQGWTGPT TCVSGTTCTV VNPYYSQCL


 


SEQ ID NO: 4
ESACTLQSET HPPLTWQKCS SGGTCTQQTG SVVIDANWRW THATNSSTNC YDGNTWSSTL CPDNETCAKN CCLDGAAYAS TYGVTTSGNS



LSIDFVTQSA QKNVGARLYL MASDTTYQEF TLLGNEFSFD VDVSQLPCGL NGALYFVSMD ADGGVSKYPT NTAGAKYGTG YCDSQCPRDL



KFINGQANVE GWEPSSNNAN TGIGGHGSCC SEMDIWQANS ISEALTPHPC TTVGQEICEG DGCGGTYSDN RYGGTCDPDG CDWNPYRLGN



TSFYGPGSSF TLDTTKKLTV VTQFETSGAI NRYYVQNGVT FQQPNAELGS YSGNELNDDY CTAEEAEFGG SSFSDKGGLT QFKKATSGGM



VLVMSLWDDY YANMLWLDST YPTNETSSTP GAVRGSCSTS SGVPAQVESQ SPNAKVTFSN IKFGPIGSTG NPSG


 


SEQ ID NO: 5
MASSFQLYKA LLFFSSLLSA VQAQKVGTQQ AEVHPGLTWQ TCTSSGSCTT VNGEVTIDAN WRWLHTVNGY TNCYTGNEWD TSICTSNEVC



AEQCAVDGAN YASTYGITTS GSSLRLNFVT QSQQKNIGSR VYLMDDEDTY TMFYLLNKEF TFDVDVSELP CGLNGAVYFV SMDADGGKSR



YATNEAGAKY GTGYCDSQCP RDLKFINGVA NVEGWESSDT NPNGGVGNHG SCCAEMDIWE ANSISTAFTP HPCDTPGQTL CTGDSCGGTY



SNDRYGGTCD PDGCDFNSYR QGNKTFYGPG LTVDTNSPVT VVTQFLTDDN TDTGTLSEIK RFYVQNGVVI PNSESTYPAN PGNSITTEFC



ESQKELFGDV DVFSAHGGMA GMGAALEQGM VLVLSLWDDN YSNMLWLDSN YPTDADPTQP GIARGTCPTD SGVPSEVEAQ YPNAYVVYSN



IKFGPIGSTF GNGGGSGPTT TVTTSTATST TSSATSTATG QAQHWEQCGG NGWTGPTVCA SPWACTVVNS WYSQCL


 


SEQ ID NO: 6
MYRAIATASA LIAAVRAQQV CSLTQESKPS LNWSKCTSSG CSNVKGSVTI DANWRWTHQV SGSTNCYTGN KWDTSVCTSG KVCAEKCCLD



GADYASTYGI TSSGDQLSLS FVTKGPYSTN IGSRTYLMED ENTYQMFQLL GNEFTFDVDV SNIGCGLNGA LYFVSMDADG GKAKYPGNKA



GAKYGTGYCD AQCPRDVKFI NGQANSDGWQ PSDSDVNGGI GNLGTCCPEM DIWEANSIST AYTPHPCTKL TQHSCTGDSC GGTYSNDRYG



GTCDADGCDF NSYRQGNKTF YGPGSGFNVD TTKKVTVVTQ FHKGSNGRLS EITRLYVQNG KVIANSESKI AGVPGNSLTA DFCTKQKKVF



NDPDDFTKKG AWSGMSDALE APMVLVMSLW HDHHSNMLWL DSTYPTDSTK LGSQRGSCST SSGVPADLEK NVPNSKVAFS NIKFGPIGST YKSDGTTPTN PTNPSEPSNT



ANPNPGTVDQ WGQCGGSNYS GPTACKSGFT CKKINDFYSQ CQ


 


SEQ ID NO: 7
MLASTFSYRM YKTALILAAL LGSGQAQQVG TSQAEVHPSM TWQSCTAGGS CTTNNGKVVI DANWRWVHKV GDYTNCYTGN TWDTTICPDD



ATCASNCALE GANYESTYGV TASGNSLRLN FVTTSQQKNI GSRLYMMKDD STYEMFKLLN QEFTFDVDVS NLPCGLNGAL YFVAMDADGG



MSKYPTNKAG AKYGTGYCDS QCPRDLKFIN GQANVEGWQP SSNDANAGTG NHGSCCAEMD IWEANSISTA FTPHPCDTPG QVMCTGDACG



GTYSSDRYGG TCDPDGCDFN SFRQGNKTFY GPGMTVDTKS KFTVVTQFIT DDGTSSGTLK EIKRFYVQNG KVIPNSESTW TGVSGNSITT



EYCTAQKSLF QDQNVFEKHG GLEGMGAALA QGMVLVMSLW DDHSANMLWL DSNYPTTASS TTPGVARGTC DISSGVPADV EANHPDAYVV



YSNIKVGPIG STFNSGGSNP GGGTTTTTTT QPTTTTTTAG NPGGTGVAQH YGQCGGIGWT GPTTCASPYT CQKLNDYYSQ CL


 


SEQ ID NO: 8
MLPSTISYRI YKNALFFAAL FGAVQAQKVG TSKAEVHPSM AWQTCAADGT CTTKNGKVVI DANWRWVHDV KGYTNCYTGN TWNAELCPDN



ESCAENCALE GADYAATYGA TTSGNALSLK FVTQSQQKNI GSRLYMMKDD NTYETFKLLN QEFTFDVDVS NLPCGLNGAL YFVSMDADGG



LSRYTGNEAG AKYGTGYCDS QCPRDLKFIN GLANVEGWTP SSSDANAGNG GHGSCCAEMD IWEANSISTA YTPHPCDTPG QAMCNGDSCG



GTYSSDRYGG TCDPDGCDFN SYRQGNKSFY GPGMTVDTKK KMTVVTQFLT NDGTATGTLS EIKRFYVQDG KVIANSESTW PNLGGNSLTN



DFCKAQKTVF GDMDTFSKHG GMEGMGAALA EGMVLVMSLW DDHNSNMLWL DSNSPTTGTS TTPGVARGSC DISSGDPKDL EANHPDASVV YSNIKVGPIG STFNSGGSNP



GGSTTTTKPA TSTTTTKATT TATTNTTGPT GTGVAQPWAQ CGGIGYSGPT QCAAPYTCTK QNDYYSQCL


 


SEQ ID NO: 9
MHPSLQTILL SALFTTAHAQ QACSSKPETH PPLSWSRCSR SGCRSVQGAV TVDANWLWTT VDGSQNCYTG NRWDTSICSS EKTCSESCCI



DGADYAGTYG VTTTGDALSL KFVQQGPYSK NVGSRLYLMK DESRYEMFTL LGNEFTFDVD VSKLGCGLNG ALYFVSMDED GGMKRFPMNK



AGAKFGTGYC DSQCPRDVKF INGMANSKDW IPSKSDANAG IGSLGACCRE MDIWEANNIA SAFTPHPCKN SAYHSCTGDG CGGTYSKNRY



SGDCDPDGCD FNSYRLGNTT FYGPGPKFTI DTTRKISVVT QFLKGRDGSL REIKRFYVQN GKVIPNSVSR VRGVPGNSIT QGFCNAQKKM



FGAHESFNAK GGMKGMSAAV SKPMVLVMSL WDDHNSNMLW LDSTYPTNSR QRGSKRGSCP ASSGRPTDVE SSAPDSTVVF SNIKFGPIGS TFSRGK


 


SEQ ID NO: 10
ESACTLQSET HPPLTWQKCS SGGTCTQQTG SVVIDANWRW THATNSSTNC YDGNTWSSTL CPDNETCAKN CCLDGAAYAS TYGVTTSGNS



LSIDFVTQSA QKNVGARLYL MASDTTYQEF TLLGNEFSFD VDVSQLPCGL NGALYFVSMD ADGGVSKYPT NTAGAKYGTG YCDSQCPRDL



KFINGQANVE GWEPSSNNAN TGIGGHGSCC SEMDIWQANS ISEALTPHPC TTVGQEICEG DGCGGTYSDN RYGGTCDPDG CDWNPYRLGN



TSFYGPGSSF TLDTTKKLTV VTQFETSGAI NRYYVQNGVT FQQPNAELGS YSGNELNDDY CTAEEAEFGG SSFSDKGGLT QFKKATSGGM



VLVMSLWDDY YANMLWLDST YPTNETSSTP GAVRGSCSTS SGVPAQVESQ SPNAKVTFSN IKFGPIGSTG NPSG


 


SEQ ID NO: 11
MHQRALLFSA LAVAANAQQV GTQKPETHPP LTWQKCTAAG SCSQQSGSVV IDANWRWLHS TKDTTNCYTG NTWNTELCPD NESCAQNCAV



DGADYAGTYG VTTSGSELKL SFVTGANVGS RLYLMQDDET YQHFNLLNNE FTFDVDVSNL PCGLNGALYF VAMDADGGMS KYPSNKAGAK



YGTGYCDSQC PRDLKFINGM ANVEGWKPSS NDKNAGVGGH GSCCPEMDIW EANSISTAVT PHPCDDVSQT MCSGDACGGT YSATRYAGTC



DPDGCDFNPF RMGNESFYGP GKIVDTKSEM TVVTQFITAD GTDTGALSEI KRLYVQNGKV IANSVSNVAD VSGNSISSDF CTAQKKAFGD



EDIFAKHGGL SGMGKALSEM VLIMSIWDDH HSSMMWLDST YPTDADPSKP GVARGTCEHG AGDPEKVESQ HPDASVTFSN IKFGPIGSTY KA


 


SEQ ID NO: 12
MYRSLIFATS LLSLAKGQLV GNLYCKGSCT AKNGKVVIDA NWRWLHVKGG YTNCYTGNEW NATACPDNKS CATNCAIDGA DYRRLRHYCE



RQLLGTEVHH QGLYSTNIGS RTYLMQDDST YQLFKFTGSQ EFTFDVDLSN LPCGLNGALY FVSMDADGGL KKYPTNKAGA KYGTGYCDAQ



CPRDLKFING EGNVEGWQPS KNDQNAGVGG HGSCCAEMDI WEANSVSTAV TPHSCSTIEQ SRCDGDGCGG TYSADRYAGV CDPDGCDFNS



YRMGVKDFYG KGKTVDTSKK FTVVTQFIGS GDAMEIKRFY VQNGKTIPQP DSTIPGVTGN SITTFFCDAQ KKAFGDKYTF KDKGGMANMP STCNGMVLVM SLWDDHYSNM



LWLDSTYPTD KNPDTDAGSG RGECAITSGV PADVESQHPD ASVIYSNIKF GPINTTFG


 


SEQ ID NO: 13
MLAKFAALAA LVASANAQAV CSLTAETHPS LNWSKCTSSG CTNVAGSITV DANWRWTHIT SGSTNCYSGN EWDTSLCSTN TDCATKCCVD



GAEYSSTYGI QTSGNSLSLQ FVTKGSYSTN IGSRTYLMNG ADAYQGFELL GNEFTFDVDV SGTGCGLNGA LYFVSMDLDG GKAKYTNNKA



GAKYGTGYCD AQCPRDLKYI NGIANVEGWT PSTNDANAGI GDHGTCCSEM DIWEANKVST AFTPHPCTTI EQHMCEGDSC GGTYSDDRYG



GTCDADGCDF NSYRMGNTTF YGEGKTVDTS SKFTVVTQFI KDSAGDLAEI KRFYVQNGKV IENSQSNVDG VSGNSITQSF CNAQKTAFGD



IDDFNKKGGL KQMGKALAKP MVLVMSIWDD HAANMLWLDS TYPVEGGPGA YRGECPTTSG VPAEVEANAP NSKVIFSNIK FGPIGSTFSG



GSSGTPPSNP SSSVKPVTST AKPSSTSTAS NPSGTGAAHW AQCGGIGFSG PTTCQSPYTC QKINDYYSQC V


 


SEQ ID NO: 14
MFKKVALTAL CFLAVAQAQQ VGREVAENHP RLPWQRCTRN GGCQTVSNGQ VVLDANWRWL HVTDGYTNCY TGNSWNSTVC SDPTTCAQRC



ALEGANYQQT YGITTNGDAL TIKFLTRSQQ TNVGARVYLM ENENRYQMFN LLNKEFTFDV DVSKVPCGIN GALYFIQMDA DGGMSKQPNN



RAGAKYGTGY CDSQCPRDIK FIDGVANSAD WTPSETDPNA GRGRYGICCA EMDIWEANSI SNAYTPHPCR TQNDGGYQRC EGRDCNQPRY



EGLCDPDGCD YNPFRMGNKD FYGPGKTVDT NRKMTVVTQF ITHDNTDTGT LVDIRRLYVQ DGRVIANPPT NFPGLMPAHD SITEQFCTDQ KNLFGDYSSF ARDGGLAHMG



RSLAKGHVLA LSIWNDHGAH MLWLDSNYPT DADPNKPGIA RGTCPTTGGT PRETEQNHPD AQVIFSNIKF GDIGSTFSGY


 


SEQ ID NO: 15
MYSAAVLATF SFLLGAGAQQ VGTSTAETHP ALTVQKCAAG GTCTDESDSI VLDANWRWLH STSGSTNCYT GNTWDTTLCP DAATCTTNCA



LDGADYEGTY GITTSGDSLK LSFVTGSNVG SRTYLMDSET TYKEFALLGN EFTFTVDVSK LPCGLNGALY FVPMDADGGM SKYPTNKAGA



KYGTGYCDAQ CPQDMKFVNG TANVEGWVPD SNSANSGTGN IGSCCSEFDV WEANSMSQAL TPHVCTVDSQ TACTGDDCAS NTGVCDGDGC



DFNPYRMGNT TFYGSGMTID TSKPFSVVTQ FITDDGTETG TLTEIKRFYV QDDVVYEQPS SDISGVSGNS ITDDFCAAQK TAFGDTDYFT



QNGGMAAMGK KMADGMVLVL SIWDDYNVNM LWLDSDYPTT KDASTPGVSR GSCATDSGVP ATVEAASGSA YVTFSSIKYG PIGSTFNAPA



DSSSSVSASS SPAPIASSSS SASIAPVSSV VAAIVSSSAQ AISSAAPVVS SSAQAISSAA PVVSSVVSSA APVATSSTKS KCSKVSSTLK



TSVAAPATSA TSAAVVATSS AASSTGSVPL YGNCTGGKTC SEGTCVVQND YYSQCVASS


 


SEQ ID NO: 16
MTWQRCTGTG GSSCTNVNGE IVIDANWRWI HATGGYTNCF DGNEWNKTAC PSNAACTKNC AIEGSDYRGT YGITTSGNSL TLKFITKGQY



STNVGSRTYL MKDTNNYEMF NLIGNEFTFD VDLSQLPCGL NGALYFVSMP EKGQGTPGAK YGTGKLSQCS VHISKTLTDA CARDLKFVGG



EANADGWQAS TSDPNAGVGK KGACCAEMDV WEANSMSTAL TPHSCQPEGY AVCEESNCGG TYSLDRYAGT CDANGCDFNP YRVGNKDFYG



KGKTVDTSKK MTVVTQFLGT GSDLTELKRF YVQDGKVISN PEPTIPGMTG NSITQKWCDT QKEVFKEEVY PFNQWGGMAS MGKGMAQGMV



LVMSLWDDHY SNMLWLDSTY PTDRDPESPG AARGECAITS GAPAEVEANN PDASVMFSNI KFGPIGSTFQ QPA


 


SEQ ID NO: 17
MQIKSYIQYL AAALPLLSSV AAQQAGTITA ENHPRMTWKR CSGPGNCQTV QGEVVIDANW RWLHNNGQNC YEGNKWTSQC SSATDCAQRC



ALDGANYQST YGASTSGDSL TLKFVTKHEY GTNIGSRFYL MANQNKYQMF TLMNNEFAFD VDLSKVECGI NSALYFVAME EDGGMASYPS



NRAGAKYGTG YCDAQCARDL KFIGGKANIE GWRPSTNDPN AGVGPMGACC AEIDVWESNA YAYAFTPHAC GSKNRYHICE TNNCGGTYSD



DRFAGYCDAN GCDYNPYRMG NKDFYGKGKT VDTNRKFTVV SRFERNRLSQ FFVQDGRKIE VPPPTWPGLP NSADITPELC DAQFRVFDDR NRFAETGGFD ALNEALTIPM



VLVMSIWDDH HSNMLWLDSS YPPEKAGLPG GDRGPCPTTS GVPAEVEAQY PNAQVVWSNI RFGPIGSTVN V


 


SEQ ID NO: 18
MRTAKFATLA ALVASAAAQQ ACSLTTERHP SLSWKKCTAG GQCQTVQASI TLDSNWRWTH QVSGSTNCYT GNKWDTSICT DAKSCAQNCC



VDGADYTSTY GITTNGDSLS LKFVTKGQYS TNVGSRTYLM DGEDKYQTFE LLGNEFTFDV DVSNIGCGLN GALYFVSMDA DGGLSRYPGN



KAGAKYGTGY CDAQCPRDIK FINGEANIEG WTGSTNDPNA GAGRYGTCCS EMDIWEANNM ATAFTPHPCT IIGQSRCEGD SCGGTYSNER



YAGVCDPDGC DFNSYRQGNK TFYGKGMTVD TTKKITVVTQ FLKDANGDLG EIKRFYVQDG KIIPNSESTI PGVEGNSITQ DWCDRQKVAF



GDIDDFNRKG GMKQMGKALA GPMVLVMSIW DDHASNMLWL DSTFPVDAAG KPGAERGACP TTSGVPAEVE AEAPNSNVVF SNIRFGPIGS



TVAGLPGAGN GGNNGGNPPP PTTTTSSAPA TTTTASAGPK AGRWQQCGGI GFTGPTQCEE PYTCTKLNDW YSQCL


 


SEQ ID NO: 19
MQIKQYLQYL AAALPLVNMA AAQRAGTQQT ETHPRLSWKR CSSGGNCQTV NAEIVIDANW RWLHDSNYQN CYDGNRWTSA CSSATDCAQK



CYLEGANYGS TYGVSTSGDA LTLKFVTKHE YGTNIGSRVY LMNGSDKYQM FTLMNNEFAF DVDLSKVECG LNSALYFVAM EEDGGMRSYS



SNKAGAKYGT GYCDAQCARD LKFVGGKANI EGWRPSTNDA NAGVGPYGAC CAEIDVWESN AYAFAFTPHG CLNNNYHVCE TSNCGGTYSE



DRFGGLCDAN GCDYNPYRMG NKDFYGKGKT VDTSRKFTVV TRFEENKLTQ FFIQDGRKID IPPPTWPGLP NSSAITPELC TNLSKVFDDR



DRYEETGGFR TINEALRIPM VLVMSIWDGH YANMLWLDSV YPPEKAGQPG AERGPCAPTS GVPAEVEAQF PNAQVIWSNI RFGPIGSTYQ V


 


SEQ ID NO: 20
MMYKKFAALA ALVAGAAAQQ ACSLTTETHP RLTWKRCTSG GNCSTVNGAV TIDANWRWTH TVSGSTNCYT GNEWDTSICS DGKSCAQTCC



VDGADYSSTY GITTSGDSLN LKFVTKHQHG TNVGSRVYLM ENDTKYQMFE LLGNEFTFDV DVSNLGCGLN GALYFVSMDA DGGMSKYSGN



KAGAKYGTGY CDAQCPRDLK FINGEANIEN WTPSTNDANA GFGRYGSCCS EMDIWDANNM ATAFTPHPCT IIGQSRCEGN SCGGTYSSER



YAGVCDPDGC DFNAYRQGDK TFYGKGMTVD TTKKMTVVTQ FHKNSAGVLS EIKRFYVQDG KIIANAESKI PGNPGNSITQ EWCDAQKVAF



GDIDDFNRKG GMAQMSKALE GPMVLVMSVW DDHYANMLWL DSTYPIDKAG TPGAERGACP TTSGVPAEIE AQVPNSNVIF SNIRFGPIGS



TVPGLDGSTP SNPTATVAPP TSTTTSVRSS TTQISTPTSQ PGGCTTQKWG QCGGIGYTGC TNCVAGTTCT ELNPWYSQCL


 


SEQ ID NO: 21
MYRNFLYAAS LLSVARSQLV GTQTTETHPG MTWQSCTAKG SCTTCSDNKA CASNCAVDGA DYKGTYGITA SGNSLQLKFI TKGSYSTNIG



SRTYLMASDT AYQMFKFDGN KEFTFDVDLS GLPCGFNGAL YFVSMDEDGG LKKYSGNKAG AKYGTGYCDA QCPRDLKFIN GEGNVEGWKP



SDNDANAGVG GHGSCCAEMD IWEANSISTA VTPHACSTIE QTRCDGDGCG GTYSADRYAG VCDPDGCDFN AYRMGVKNFY GKGMTVDTSK



KFTVVTQFIG TGDAMEIKRF YVQGGKTIEQ PASTIPGVEG NSITTKFCDQ QKQVFGDRYT YKEKGGTANM AKALAQGMVL VMSLWDDHYS NMLWLDSTYP TDKNPDTDLG



SGRGSCDVKS GAPADVESKS PDATVIYSNI KFGPLNSTY


 


SEQ ID NO: 22
MLGKIAIASL SFLAIAKGQQ VGREVAENHP RLPWQRCTRN GGCQTVSNGQ VVLDANWRWL HVTDGYTNCY TGNSWNSSVC SDGTTCAQRC



ALEGANYQQT YGITTSGNSL TMKFLTRSQG TNVGGRVYLM ENENRYQMFN LLNKEFTFDV DVSKVPCGIN GALYFIQMDA DGGMSSQPNN



RAGAKYGTGY CDSQCPRDIK FIDGVANSVG WEPSETDSNA GRGRYGICCA EMDIWEANSI SNAYTPHPCR TQNDGGYQRC EGRDCNQPRY



EGLCDPDGCD YNPFRMGNKD FYGPGKTIDT NRKMTVVTQF ITHDNTDTGT LVDIRRLYVQ DGRVIANPPT NFPGLMPAHD SITEQFCTDQ



KNLFGDYSSF ARDGGLAHMG RSLAKGHVLA LSIWNDHGAH MLWLDSNYPT DADPNKPGIA RGTCPTTGGT PRETEQNHPD AQVIFSNIKF



GDIGSTFSGY


 


SEQ ID NO: 23
MFPRSILLAL SLTAVALGQQ VGTNMAENHP SLTWQRCTSS GCQNVNGKVT LDANWRWTHR INDFTNCYTG NEWDTSICPD GVTCAENCAL



DGADYAGTYG VTSSGTALTL KFVTESQQKN IGSRLYLMAD DSNYEIFNLL NKEFTFDVDV SKLPCGLNGA LYFSEMAADG GMSSTNTAGA



KYGTGYCDSQ CPRDIKFIDG EANSEGWEGS PNDVNAGTGN FGACCGEMDI WEANSISSAY TPHPCREPGL QRCEGNTCSV NDRYATECDP



DGCDFNSFRM GDKSFYGPGM TVDTNQPITV VTQFITDNGS DNGNLQEIRR IYVQNGQVIQ NSNVNIPGID SGNSISAEFC DQAKEAFGDE



RSFQDRGGLS GMGSALDRGM VLVLSIWDDH AVNMLWLDSD YPLDASPSQP GISRGTCSRD SGKPEDVEAN AGGVQVVYSN IKFGDINSTF



NNNGGGGGNP SPTTTRPNSP AQTMWGQCGG QGWTGPTACQ SPSTCHVIND FYSQCF


 


SEQ ID NO: 24
MYRNLALASL SLFGAARAQQ AGTVTTETHP SLSWKTCTGT GGTSCTTKAG KITLDANWRW THVTTGYTNC YDGNSWNTTA CPDGATCTKN



CAVDGADYSG TYGITTSSNS LSIKFVTKGS NSANIGSRTY LMESDTKYQM FNLIGQEFTF DVDVSKLPCG LNGALYFVEM AADGGIGKGN



NKAGAKYGTG YCDSQCPHDI KFINGKANVE GWNPSDADPN AGSGKIGACC PEMDIWEANS ISTAYTPHPC KGTGLQECTD DVSCGDGSNR



YSGLCDKDGC DFNSYRMGVK DFYGPGATLD TTKKMTVVTQ FLGSGSTLSE IKRFYVQNGK VFKNSDSAIE GVTGNSITES FCAAQKTAFG DTNSFKTLGG LNEMGASLAR



GHVLVMSLWD DHAVNMLWLD STYPTNSTKL GAQRGTCAID SGKPEDVEKN HPDATVVFSD IKFGPIGSTF QQPS


 


SEQ ID NO: 25
MVDIQIATFL LLGVVGVAAQ QVGTYIPENH PLLATQSCTA SGGCTTSSSK IVLDANRRWI HSTLGTTSCL TANGWDPTLC PDGITCANYC



ALDGVSYSST YGITTSGSAL RLQFVTGTNI GSRVFLMADD THYRTFQLLN QELAFDVDVS KLPCGLNGAL YFVAMDADGG KSKYPGNRAG



AKYGTGYCDS QCPRDVQFIN GQANVQGWNA TSATTGTGSY GSCCTELDIW EANSNAAALT PHTCTNNAQT RCSGSNCTSN TGFCDADGCD



FNSFRLGNTT FLGAGMSVDT TKTFTVVTQF ITSDNTSTGN LTEIRRFYVQ NGNVIPNSVV NVTGIGAVNS ITDPFCSQQK KAFIETNYFA



QHGGLAQLGQ ALRTGMVLAF SISDDPANHM LWLDSNFPPS ANPAVPGVAR GMCSITSGNP ADVGILNPSP YVSFLNIKFG SIGTTFRPA


 


SEQ ID NO: 26
MHQRALLFSA LAVAANAQQV GTQTPETHPP LTWQKCTAAG SCSQQSGSVV IDANWRWLHS TKDTTNCYTG NTWNTELCPD NESCAQNCAL



DGADYAGTYG VTTSGSELKL SFVTGANVGS RLYLMQDDET YQHFNLLNHE FTFDVDVSNL PCGLNGALYF VAMDADGGMS KYPSNKAGAK



YGTGYCDSQC PRDLKFINGM ANVEGWEPSS SDKNAGVGGH GSCCPEMDIW EANSISTAVT PHPCDDVSQT MCSGDACGGT YSESRYAGTC



DPDGCDFNPF RMGNESFYGP GKIVDTKSKM TVVTQFITAD GTDSGALSEI KRLYVQNGKV IANSVSNVAG VSGNSITSDF CTAQKKAFGD EDIFAKHGGL SGMGKALSEM



VLIMSIWDDH HSSMMWLDST YPTDADPSKP GVARGTCEHG AGDPENVESQ HPDASVTFSN IKFGPIGSTY EG


 


SEQ ID NO: 27
MFRTATLLAF TMAAMVFGQQ VGTNTAENHR TLTSQKCTKS GGCSNLNTKI VLDANWRWLH STSGYTNCYT GNQWDATLCP DGKTCAANCA



LDGADYTGTY GITASGSSLK LQFVTGSNVG SRVYLMADDT HYQMFQLLNQ EFTFDVDMSN LPCGLNGALY LSAMDADGGM AKYPTNKAGA



KYGTGYCDSQ CPRDIKFING EANVEGWNAT SANAGTGNYG TCCTEMDIWE ANNDAAAYTP HPCTTNAQTR CSGSDCTRDT GLCDADGCDF



NSFRMGDQTF LGKGLTVDTS KPFTVVTQFI TNDGTSAGTL TEIRRLYVQN GKVIQNSSVK IPGIDPVNSI TDNFCSQQKT AFGDTNYFAQ



HGGLKQVGEA LRTGMVLALS IWDDYAANML WLDSNYPTNK DPSTPGVARG TCATTSGVPA QIEAQSPNAY VVFSNIKFGD LNTTYTGTVS



SSSVSSSHSS TSTSSSHSSS STPPTQPTGV TVPQWGQCGG IGYTGSTTCA SPYTCHVLNP YYSQCY


 


SEQ ID NO: 28
MYQRALLFSF FLAAARAHEA GTVTAENHPS LTWQQCSSGG SCTTQNGKVV IDANWRWVHT TSGYTNCYTG NTWDTSICPD DVTCAQNCAL



DGADYSGTYG VTTSGNALRL NFVTQSSGKN IGSRLYLLQD DTTYQIFKLL GQEFTFDVDV SNLPCGLNGA LYFVAMDADG NLSKYPGNKA



GAKYGTGYCD SQCPRDLKFI NGQANVEGWQ PSANDPNAGV GNHGSSCAEM DVWEANSIST AVTPHPCDTP GQTMCQGDDC GGTYSSTRYA



GTCDPDGCDF NPYQPGNHSF YGPGKIVDTS SKFTVVTQFI TDDGTPSGTL TEIKRFYVQN GKVIPQSEST ISGVTGNSIT TEYCTAQKAA



FGDNTGFFTH GGLQKISQAL AQGMVLVMSL WDDHAANMLW LDSTYPTDAD PDTPGVARGT CPTTSGVPAD VESQNPNSYV IYSNIKVGPI NSTFTAN


 


SEQ ID NO: 29
MQIKSYIQYL AAALPLLSSV AAQQAGTITA ENHPRMTWKR CSGPGNCQTV QGEVVIDANW RWLHNNGQNC YEGNKWTSQC SSATDCAQRC



ALDGANYQST YGASTSGDSL TLKFVTKHEY GTNIGSRFYL MANQNKYQMF TLMNNEFAFD VDLSKVECGI NSALYFVAME EDGGMASYPS



NRAGAKYGTG YCDAQCARDL KFIGGKANIE GWRPSTNDPN AGVGPMGACC AEIDVWESNA YAYAFTPHAC GSKNRYHICE TNNCGGTYSD



DRFAGYCDAN GCDYNPYRMG NKDFYGKGKT VDTNRKFTVV SRFERNRLSQ FFVQDGRKIE VPPPTWPGLP NSADITPELC DAQFRVFDDR



NRFAETGGFD ALNEALTIPM VLVMSIWDDH HSNMLWLDSS YPPEKAGLPG GDRGPCPTTS GVPAEVEAQY PDAQVVWSNI RFGPIGSTVN V


 


SEQ ID NO: 30
MYRKLAVISA FLATARAQSA CTLQSETHPP LTWQKCSSGG TCTQQTGSVV IDANWRWTHA TNSSTNCYDG NTWSSTLCPD NETCAKNCCL



DGAAYASTYG VTTSGNSLSI GFVTQSAQKN VGARLYLMAS DTTYQEFTLL GNEFSFDVDV SQLPCGLNGA LYFVSMDADG GVSKYPTNTA



GAKYGTGYCD SQCPRDLKFI NGQANVEGWE PSSNNANTGI GGHGSCCSEM DIWEANSISE ALTPHPCTTV GQEICEGDGC GGTYSDNRYG



GTCDPDGCDW DPYRLGNTSF YGPGSSFTLD TTKKLTVVTQ FETSGAINRY YVQNGVTFQQ PNAELGSYSG NGLNDDYCTA EEAEFGGSSF



SDKGGLTQFK KATSGGMVLV MSLWDDYYAN MLWLDSTYPT NETSSTPGAV RGSCSTSSGV PAQVESQSPN AKVTFSNIKF GPIGSTGDPS



GGNPPGGNPP GTTTTRRPAT TTGSSPGPTQ SHYGQCGGIG YSGPTVCASG TTCQVLNPYY SQCL


 


SEQ ID NO: 31
MYQRALLFSF FLAAARAQQA GTVTAENHPS LTWQQCSSGG SCTTQNGKVV IDANWRWVHT TSGYTNCYTG NTWDTSICPD DVTCAQNCAL



DGADYSGTYG VTTSGNALRL NFVTQSSGKN IGSRLYLLQD DTTYQIFKLL GQEFTFDVDV SNLPCGLNGA LYFVAMDADG GLSKYPGNKA



GAKYGTGYCD SQCPRDLKFI NGQANVEGWQ PSANDPNAGV GNHGSCCAEM DVWEANSIST AVTPHPCDTP GQTMCQGDDC GGTYSSTRYA



GTCDPDGCDF NPYRQGNHSF YGPGQIVDTS SKFTVVTQFI TDDGTPSGTL TEIKRFYVQN GKVIPQSEST ISGVTGNSIT TEYCTAQKAA FGDNTGFFTH GGLQKISQAL



AQGMVLVMSL WDDHAANMLW LDSTYPTDAD PDTPGVARGT CPTTSGVPAD VESQYPNSYV IYSNIKVGPI NSTFTAN


 


SEQ ID NO: 32
MIRKITTLAA LVGVVRGQAA CSLTAETHPS LTWQKCSSGG SCTNVAGSVT IDANWRWTHT TSGYTNCYTG NKWDTSICST NADCASKCCV



DGANYQQTYG ASTSGNALSL QYVTQSSGKN VGSRLYLLES ENKYQMFNLL GNEFTFDVDA SKLGCGLNGA VYFVSMDADG GQSKYSGNKA



GAKYGTGYCD SQCPRDLKYI NGAANVEGWQ PSSGDANSGV GNMGSCCAEM DIWEANSIST AYTPHPCSNN AQHSCKGDDC GGTYSSVRYA



GDCDPDGCDF NSYRQGNRTF YGPGSNFNVD SSKKVTVVTQ FISSGGQLTD IKRFYVQNGK VIPNSQSTIT GVTGNSVTQD YCDKQKTAFG



DQNVFNQRGG LRQMGDALAK GMVLVMSVWD DHHSQMLWLD STYPTTSTAP GAARGSCSTS SGKPSDVQSQ TPGATVVYSN IKFGPIGSTF KSS


 


SEQ ID NO: 33
MLRRALLLSS SAILAVKAQQ AGTATAENHP PLTWQECTAP GSCTTQNGAV VLDANWRWVH DVNGYTNCYT GNTWDPTYCP DDETCAQNCA



LDGADYEGTY GVTSSGSSLK LNFVTGSNVG SRLYLLQDDS TYQIFKLLNR EFSFDVDVSN LPCGLNGALY FVAMDADGGV SKYPNNKAGA



KYGTGYCDSQ CPRDLKFIDG EANVEGWQPS SNNANTGIGD HGSCCAEMDV WEANSISNAV TPHPCDTPGQ TMCSGDDCGG TYSNDRYAGT



CDPDGCDFNP YRMGNTSFYG PGKIIDTTKP FTVVTQFLTD DGTDTGTLSE IKRFYIQNSN VIPQPNSDIS GVTGNSITTE FCTAQKQAFG



DTDDFSQHGG LAKMGAAMQQ GMVLVMSLWD DYAAQMLWLD SDYPTDADPT TPGIARGTCP TDSGVPSDVE SQSPNSYVTY SNIKFGPINS TFTAS


 


SEQ ID NO: 34
MHQRALLFSA FWTAVQAQQA GTLTAETHPS LTWQKCAAGG TCTEQKGSVV LDSNWRWLHS VDGSTNCYTG NTWDATLCPD NESCASNCAL



DGADYEGTYG VTTSGDALTL QFVTGANIGS RLYLMADDDE SYQTFNLLNN EFTFDVDASK LPCGLNGAVY FVSMDADGGV AKYSTNKAGA



KYGTGYCDSQ CPRDLKFING QVRKGWEPSD SDKNAGVGGH GSCCPQMDIW EANSISTAYT PHPCDDTAQT MCEGDTCGGT YSSERYAGTC



DPDGCDFNAY RMGNESFYGP SKLVDSSSPV TVVTQFITAD GTDSGALSEI KRFYVQGGKV IANAASNVDG VTGNSITADF CTAQKKAFGD



DDIFAQHGGL QGMGNALSSM VLTLSIWDDH HSSMMWLDSS YPEDADATAP GVARGTCEPH AGDPEKVESQ SGSATVTYSN IKYGPIGSTF DAPA


 


SEQ ID NO: 35
MASTLSFKIY KNALLLAAFL GAAQAQQVGT STAEVHPSLT WQKCTAGGSC TSQSGKVVID SNWRWVHNTG GYTNCYTGND WDRTLCPDDV



TCATNCALDG ADYKGTYGVT ASGSSLRLNF VTQASQKNIG SRLYLMADDS KYEMFQLLNQ EFTFDVDVSN LPCGLNGALY FVAMDEDGGM



ARYPTNKAGA KYGTGYCDAQ CPRDLKFING QANVEGWEPS SSDVNGGTGN YGSCCAEMDI WEANSISTAF TPHPCDDPAQ TRCTGDSCGG



TYSSDRYGGT CDPDGCDFNP YRMGNQSFYG PSKIVDTESP FTVVTQFITN DGTSTGTLSE IKRFYVQNGK VIPQSVSTIS AVTGNSITDS



FCSAQKTAFK DTDVFAKHGG MAGMGAGLAE GMVLVMSLWD DHAANMLWLD STYPTSASST TPGAARGSCD ISSGEPSDVE ANHSNAYVVY



SNIKVGPLGS TFGSTDSGSG TTTTKVTTTT ATKTTTTTGP STTGAAHYAQ CGGQNWTGPT TCASPYTCQR QGDYYSQCL


 


SEQ ID NO: 36
MVSAKFAALA ALVASASAQQ VCSLTPESHP PLTWQRCSAG GSCTNVAGSV TLDSNWRWTH TLQGSTNCYS GNEWDTSICT TGTKCAQNCC



VEGAEYAATY GITTSGNQLN LKFVTEGKYS TNVGSRTYLM ENATKYQGFN LLGNEFTFDV DVSNIGCGLN GALYFVSMDL DGGLAKYSGN



KAGAKYGTGY CDAQCPRDIK FINGEANIEG WNPSTNDVNA GAGRYGTCCS EMDIWEANNM ATAYTPHSCT ILDQSRCEGE SCGGTYSSDR



YGGVCDPDGC DFNSYRMGNK EFYGKGKTVD TTKKMTVVTQ FLKNAAGELS EIKRFYVQNG VVIPNSVSSI PGVPNQNSIT QDWCDAQKIA



FGDPDDNTAK GGLRQMGLAL DKPMVLVMSI WNDHAAHMLW LDSTYPVDAA GRPGAERGAC PTTSGVPSEV EAEAPNSNVA FSNIKFGPIG STFNSGSTNP NPISSSTATT



PTSTRVSSTS TAAQTPTSAP GGTVPRWGQC GGQGYTGPTQ CVAPYTCVVS NQWYSQCL


 


SEQ ID NO: 37
MFPYIALVSF SFLSVVLAQQ VGTLTAETHP QLTVQQCTRG GSCTTQQRSV VLDGNWRWLH STSGSNNCYT GNTWDTSLCP DAATCSRNCA



LDGADYSGTY GITSSGNALT LKFVTHGPYS TNIGSRVYLL ADDSHYQMFN LKNKEFTFDV DVSQLPCGLN GALYFSQMDA DGGTGRFPNN



KAGAKYGTGY CDSQCPHDIK FINGEANVQG WQPSPNDSNA GKGQYGSCCA EMDIWEANSM ASAYTPHPCT VTTPTRCQGN DCGDGDNRYG



GVCDKDGCDF NSFRMGDKNF LGPGKTVNTN SKFTVVTQFL TSDNTTSGTL SEIRRLYVQN GRVIQNSKVN IPGMASTLDS ITESFCSTQK



TVFGDTNSFA SKGGLRAMGN AFDKGMVLVL SIWDDHEAKM LWLDSNYPLD KSASAPGVAR GTCATTSGEP KDVESQSPNA QVIFSNIKYG DIGSTYSN


 


SEQ ID NO: 38
MYRAIATASA LIAAVRAQQV CSLTQESKPS LNWSKCTSSG CSNVKGSVTI DANWRWTHQV SGSTNCYTGN KWDTSVCTSG KVCAERCCLD



GADYASTYGI TSSGDQLSLS FVTKGPYSTN IGSRTYLMED ENTYQMFQLL GNEFTFDVDV SNIGCGLNGA LYFVSMDADG GKAKYPGNKA



GAKYGTGYCD AQCPRDVKFI NGQANSDGWQ PSDSDVNGGI GNLGTCCPEM DIWEANSIST AYTPHPCTKL TQHSCTGDSC GGTYSNDRYG



GTCDADGCDF NSYRQGNKTF YGPGSGFNVD TTKKVTVVTQ FHKGSNGRLS EITRLYVQNG KVIANSESKI AGVPGNSLTA DFCTKQKKVF



NDPDDFTKKG AWSGMSDALE APMVLVMSLW HDHHSNMLWL DSTYPTDSTK LGSQRGSCST SSGVPADLEK NVPNSKVAFS NIKFGPIGST



YKSDGTTPTN PTNPSEPSNT ANPNPGTVDQ WGQCGGSNYS GPTACKSGFT CKKINDFYSQ CQ


 


SEQ ID NO: 39
MYSAAVLATF SFLLGAGAQQ VGTLKTESHP PLTIQKCAAG GTCTDEADSV VLDANWRWLH STSGSTNCYT GNTWDTTLCP DAATCTANCA



FDGADYEGTY GITSSGDSLK LSFVTGSNVG SRTYLMDSET TYKEFALLGN EFTFTVDVSK LPCGLNGALY FVPMDADGGM SKYPTNKAGA



KYGTGYCDAQ CPQDMKFVSG GANNEGWVPD SNSANSGTGN IGSCCSEFDV WEANSMSQAL TPHTCTVDGQ TACTGDDCAG NTGVCDADGC



DFNPYRMGNT TFYGSGKTID TTKPFSVVTQ FITDDGTETG TLTEIKRFYV QDDVVYEQPN SDISGVSGNS ITDDFCTAQK TAFGDTDYFS



QKGGMAAMGK KMADGMVLVL SIWDDYNVNM LWLDSDYPTT KDASTPGVSR GSCATTSGVP ATVEAASGSA YVTFSSIKYG PIGSTFKAPA



DSSSPVVASS SPAAVAAVVS TSSAQAVPSH PAVSSSQAAV STPEAVSSAP EVPASSSAAQ SVAPTSTKPK CSKVSQSSTL ATSVAAPATT ATSAAVAATS AASSSGSVPL



YGNCTGGKTC SEGTCVVQNP WYSQCVASS


 


SEQ ID NO: 40
MFRAAALLAF TCLAMVSGQQ AGTNTAENHP QLQSQQCTTS GGCKPLSTKV VLDSNWRWVH STSGYTNCYT GNEWDTSLCP DGKTCAANCA



LDGADYSGTY GITSTGTALT LKFVTGSNVG SRVYLMADDT HYQLLKLLNQ EFTFDVDMSN LPCGLNGALY LSAMDADGGM SKYPGNKAGA



KYGTGYCDSQ CPKDIKFING EANVGNWTET GSNTGTGSYG TCCSEMDIWE ANNDAAAFTP HPCTTTGQTR CSGDDCARNT GLCDGDGCDF



NSFRMGDKTF LGKGMTVDTS KPFTVVTQFL TNDNTSTGTL SEIRRIYIQN GKVIQNSVAN IPGVDPVNSI TDNFCAQQKT AFGDTNWFAQ



KGGLKQMGEA LGNGMVLALS IWDDHAANML WLDSDYPTDK DPSAPGVARG TCATTSGVPS DVESQVPNSQ VVFSNIKFGD IGSTFSGTSS



PNPPGGSTTS SPVTTSPTPP PTGPTVPQWG QCGGIGYSGS TTCASPYTCH VLNPYYSQCY


 


SEQ ID NO: 41
MYRKLAVISA FLATARAQSA CTLQSETHPP LTWQKCSSGG TCTQQTGSVV IDANWRWTHA TNSSTNCYDG NTWSSTLCPD NETCAKNCCL



DGAAYASTYG VTTSGNSLSI GFVTQSAQKN VGARLYLMAS DTTYQEFTLL GNEFSFDVDV SQLPCGLNGA LYFVSMDADG GVSKYPTNTA



GAKYGTGYCD SQCPRDLKFI NGQANVEGWE PSSNNANTGI GGHGSCCSEM DIWEANSISE ALTPHPCTTV GQEICEGDGC GGTYSDNRYG



GTCDPDGCDW NPYRLGNTSF YGPGSSFTLD TTKKLTVVTQ FETSGAINRY YVQNGVTFQQ PNAELGSYSG NELNDDYCTA EEAEFGGSSF



SDKGGLTQFK KATSGGMVLV MSLWDDYYAN MLWLDSTYPT NETSSTPGAV RGSCSTSSGV PAQVESQSPN AKVTFSNIKF GPIGSTGNPS



GGNPPGGNRG TTTTRRPATT TGSSPGPTQS HYGQCGGIGY SGPTVCASGT TCQVLNPYYS QCL


 


SEQ ID NO: 42
MPSTYDIYKK LLLLASFLSA SQAQQVGTSK AEVHPSLTWQ TCTSGGSCTT VNGKVVVDAN WRWVHNVDGY NNCYTGNTWD TTLCPDDETC



ASNCALEGAD YSGTYGVTTS GNSLRLNFVT QASQKNIGSR LYLMEDDSTY KMFKLLNQEF TFDVDVSNLP CGLNGAVYFV SMDADGGMAK



YPANKAGAKY GTGYCDSQCP RDLKFINGMA NVEGWEPSAN DANAGTGNHG SCCAEMDIWE ANSISTAYTP HPCDTPGQVM CTGDSCGGTY



SSDRYGGTCD PDGCDFNSYR QGNKTFYGPG MTVDTKSKIT VVTQFLTNDG TASGTLSEIK RFYVQNGKVI PNSESTWSGV SGNSITTAYC



NAQKTLFGDT DVFTKHGGME GMGAALAEGM VLVLSLWDDH NSNMLWLDSN YPTDKPSTTP GVARGSCDIS SGDPKDVEAN DANAYVVYSN IKVGPIGSTF SGSTGGGSSS



STTATSKTTT TSATKTTTTT TKTTTTTSAS STSTGGAQHW AQCGGIGWTG PTTCVAPYTC QKQNDYYSQC L


 


SEQ ID NO: 43
MISKVLAFTS LLAAARAQQA GTLTTETHPP LSVSQCTASG CTTSAQSIVV DANWRWLHST TGSTNCYTGN TWDKTLCPDG ATCAANCALD



GADYSGVYGI TTSGNSIKLN FVTKGANTNV GSRTYLMAAG STTQYQMLKL LNQEFTFDVD VSNLPCGLNG ALYFAAMDAD GGLSRFPTNK



AGAKYGTGYC DAQCPQDIKF INGVANSVGW TPSSNDVNAG AGQYGSCCSE MDIWEANKIS AAYTPHPCSV DTQTRCTGTD CGIGARYSSL



CDADGCDFNS YRQGNTSFYG AGLTVNTNKV FTVVTQFITN DGTASGTLKE IRRFYVQNGV VIPNSQSTIA GVPGNSITDS FCAAQKTAFG



DTNEFATKGG LATMSKALAK GMVLVMSIWD DHTANMLWLD APYPATKSPS APGVTRGSCS ATSGNPVDVE ANSPGSSVTF SNIKWGPINS



TYTGSGAAPS VPGTTTVSSA PASTATSGAG GVAKYAQCGG SGYSGATACV SGSTCVALNP YYSQCQ


 


SEQ ID NO: 44
MFPAATLFAF SLFAAVYGQQ VGTQLAETHP RLTWQKCTRS GGCQTQSNGA IVLDANWRWV HNVGGYTNCY TGNTWNTSLC PDGATCAKNC



ALDGANYQST YGITTSGNAL TLKFVTQSEQ KNIGSRVYLL ESDTKYQLFN PLNQEFTFDV DVSQLPCGLN GAVYFSAMDA DGGMSKFPNN



AAGAKYGTGY CDSQCPRDIK FINGEANVQG WQPSPNDTNA GTGNYGACCN EMDVWEANSI STAYTPHPCT QQGLVRCSGT ACGGGSNRYG



SICDPDGCDF NSFRMGDKSF YGPGLTVNTQ QKFTVVTQFL TNNNSSSGTL REIRRLYVQN GRVIQNSKVN IPGMPSTMDS VTTEFCNAQK TAFNDTFSFQ QKGGMANMSE



ALRRGMVLVL SIWDDHAANM LWLDSNYPTD RPASQPGVAR GTCPTSSGKP SDVENSTANS QVIYSNIKFG DIGSTYSA


 


SEQ ID NO: 45
MKGSISYQIY KGALLLSALL NSVSAQQVGT LTAETHPALT WSKCTAGXCS QVSGSVVIDA NWPXVHSTSG STNCYTGNTW DATLCPDDVT



CAANCAVDGA RRQHLRVTTS GNSLRINFVT TASQKNIGSR LYLLENDTTY QKFNLLNQEF TFDVDVSNLP CGLNGALYFV DMDADGGMAK



YPTNKAGAKY GTGYCDSQCP RDLKFINGQA NVDGWTPSKN DVNSGIGNHG SCCAEMDIWE ANSISNAVTP HPCDTPSQTM CTGQRCGGTY



STDRYGGTCD PDGCDFNPYR MGVTNFYGPG ETIDTKSPFT VVTQFLTNDG TSTGTLSEIK RFYVQGGKVI GNPQSTIVGV SGNSITDSWC



NAQKSAFGDT NEFSKHGGMA GMGAGLADGM VLVMSLWDDH ASDMLWLDST YPTNATSTTP GAKRGTCDIS RRPNTVESTY PNAYVIYSNI



KTGPLNSTFT GGTTSSSSTT TTTSKSTSTS SSSKTTTTVT TTTTSSGSSG TGARDWAQCG GNGWTGPTTC VSPYTCTKQN DWYSQCL


 


SEQ ID NO: 46
MFRTAALTAF TLAAVVLGQQ VGTLTAENHP ALSIQQCTAS GCTTQQKSVV LDSNWRWTHS LPVHTNCYTG NAWDASLCPD PTTCATNCAI



DGADYSGTYG ITTSGNALTL RFVTNGPYSK NIGSRVYLLD DADHYKMFDL KNQEFTFDVD MSGLPCGLNG ALYFSEMPAD GGKAAHTSNK



AGAKYGTGYC DAQCPHDIKW INGEANILDW SASATDANAG NGRYGACCAE MDIWEANSEA TAYTPHVCRD EGLYRCSGTE CGDGDNRYGG



VCDKDGCDFN SYRMGDKNFL GRGKTIDTTK KITVVTQFIT DDNTSSGNLV EIRRVYVQDG VTYQNSFSTF PSLSQYNSIS DDFCVAQKTL



FGDNQYYNTH GGTEKMGDAM ANGMVLIMSL WSDHAAHMLW LDSDYPLDKS PSEPGVSRGA CATTTGDPDD VVANHPNASV TFSNIKYGPI



GSTYGGSTPP VSSGNTSAPP VTSTTSSGPT TPTGPTGTVP KWGQCGGNGY SGPTTCVAGS TCTYSNDWYS QCL


 


SEQ ID NO: 47
MYQRALLFSA LLSVSRAQQA GTAQEEVHPS LTWQRCEASG SCTEVAGSVV LDSNWRWTHS VDGYTNCYTG NEWDATLCPD NESCAQNCAV



DGADYEATYG ITSNGDSLTL KFVTGSNVGS RVYLMEDDET YQMFDLLNNE FTFDVDVSNL PCGLNGALYF TSMDADGGLS KYEGNTAGAK



YGTGYCDSQC PRDIKFINGL GNVEGWEPSD SDANAGVGGM GTCCPEMDIW EANSISTAYT PHPCDSVEQT MCEGDSCGGT YSDDRYGGTC



DPDGCDFNSY RMGNTSFYGP GAIIDTSSKF TVVTQFIADG GSLSEIKRFY VQNGEVIPNS ESNISGVEGN SITSEFCTAQ KTAFGDEDIF AQHGGLSAMG DAASAMVLIL



SIWDDHHSSM MWLDSSYPTD ADPSQPGVAR GTCEQGAGDP DVVESEHADA SVTFSNIKFG PIGSTF


 


SEQ ID NO: 48
MYRAIATASA LIAAVRAQQV CSLTTETKPA LTWSKCTSSG CSNVQGSVTI DANWRWTHQV SGSTNCHTGN KWDTSVCTSG KVCAEKCCVD



GADYASTYGI TSSGNQLSLS FVTKGSYGTN IGSRTYLMED ENTYQMFQLL GNEFTFDVDV SNIGCGLNGA LYFVSMDADG GKAKYPGNKA



GAKYGTGYCD AQCPRDVKFI NGQANSDGWE PSKSDVNGGI GNLGTCCPEM DIWEANSIST AYTPHPCTKL TQHACTGDSC GGTYSNDRYG



GTCDADGCDF NAYRQGNKTF YGPGSGFNVD TTKKVTVVTQ FHKGSNGRLS EITRLYVQNG KVIANSESKI AGNPGSSLTS DFCTTQKKVF



GDIDDFAKKG AWNGMSDALE APMVLVMSLW HDHHSNMLWL DSTYPTDSTA LGSQRGSCST SSGVPADLEK NVPNSKVAFS NIKFGPIGST



YNKEGTQPQP TNPTNPNPTN PTNPGTVDQW GQCGGTNYSG PTACKSPFTC KKINDFYSQC Q


 


SEQ ID NO: 49
MFRTAALTAF TLAAVVLGQQ VGTLAAENHP ALSIQQCTAS GCTTQQKSVV LDSNWRWTHS TAGATNCYTG NAWDSSLCPN PTTCATNCAI



DGADYSGTYG ITTSGNSLTL RFVTNGQYSE NIGSRVYLLD DADHYKLFNL KNQEFTFDVD MSGLPCGLNG ALYFSEMAAD GGKAAHTGNN



AGAKYGTGYC DAQCPHDIKW INGEANILDW SGSATDPNAG NGRYGACCAE MDIWEANSEA TAYTPHVCRD EGLYRCSGTE CGDGDNRYGG



VCDKDGCDFN SYRMGDKNFL GRGKTIDTTK KITVVTQFIT DDNTPTGNLV EIRRVYVQDG VTYQNSFSTF PSLSQYNSIS DDFCVAQKTL



FGDNQYYNTH GGTEKMGDSL ANGMVLIMSL WSDHAAHMLW LDSDYPLDKS PSEPGVSRGA CATTTGDPDD VVANHPNASV TFSNIKYGPI GSTYGGSTPP VSSGNTSVPP



VTSTTSSGPT TPTGPTGTVP KWGQCGGIGY SGPTSCVAGS TCTYSNEWYS QCL


 


SEQ ID NO: 50
MYQKLALISA FLATARAQSA CTLQAETHPP LTWQKCSSGG TCTQQTGSVV IDANWRWTHA TNSSTNCYDG NTWSSTLCPD NETCAKNCCL



DGAAYASTYG VTTSADSLSI GFVTQSAQKN VGARLYLMAS DTTYQEFTLL GNEFSFDVDV SQLPCGLNGA LYFVSMDADG GVTKYPTNTA



GAKYGTGYCD SQCPRDLKFI NGQANVEGWE PSSNNANTGI GGHGSCCSEM DIWEANSISE ALTPHPCTTV GQEICEGDSC GGTYSGDRYG



GTCDPDGCDW NPYRLGNTSF YGPGSSFTLD TTKKLTVVTQ FETSGAINRY YVQNGVTFQQ PNAELGDYSG NSLDDDYCAA EEAEFGGSSF



SDKGGLTQFK KATSGGMVLV MSLWDDYYAN MLWLDSTYPT DETSSTPGAV RGSSSTSSGV PAQLESNSPN AKVVYSNIKF GPIGSTGNPS



GGNPPGGNPP GTTTPRPATS TGSSPGPTQT HYGQCGGIGY IGPTVCASGS TCQVLNPYYS QCL


 


SEQ ID NO: 51
MTWQSCTAKG SCTNKNGKIV IDANWRWLHK KEGYDNCYTG NEWDATACPD NKACAANCAV DGADYSGTYG ITAGSNSLKL KFITKGSYST



NIGSRTYLMK DDTTYEMFKF TGNQEFTFDV DVSNLPCGFN GALYFVSMDA DGGLKKYSTN KAGAKYGTGY CDAQCPRDLK FINGEGNVEG



WKPSSNDANA GVGGHGSCCA EMDIWEANSV STAVTPHSCS TIEQSRCDGD GCGGTYSADR YAGVCDPDGC DFNSYRMGVK DFYGKGKTVD



TSKKFTVVTQ FIGTGDAMEI KRFYVQNGKT IAQPASAVPG VEGNSITTKF CDQQKAVFGD TYTFKDKGGM ANMAKALANG MVLVMSLWDD



HYSNMLWLDS TYPTDKNPDT DLGTGRGECE TSSGVPADVE SQHADATVVY SNIKFGPLNS TFG


 


SEQ ID NO: 52
MASAISFQVY RSALILSAFL PSITQAQQIG TYTTETHPSM TWETCTSGGS CATNQGSVVM DANWRWVHQV GSTTNCYTGN TWDTSICDTD



ETCATECAVD GADYESTYGV TTSGSQIRLN FVTQNSNGAN VGSRLYMMAD NTHYQMFKLL NQEFTFDVDV SNLPCGLNGA LYFVTMDEDG



GVSKYPNNKA GAQYGVGYCD SQCPRDLKFI QGQANVEGWT PSSNNENTGL GNYGSCCAEL DIWESNSISQ ALTPHPCDTA TNTMCTGDAC



GGTYSSDRYA GTCDPDGCDF NPYRMGNTTF YGPGKTIDTN SPFTVVTQFI TDDGTDTGTL SEIRRYYVQN GVTYAQPDSD ISGITGNAIN



ADYCTAENTV FDGPGTFAKH GGFSAMSEAM STGMVLVMSL WDDYYADMLW LDSTYPTNAS SSTPGAVRGS CSTDSGVPAT IESESPDSYV TYSNIKVGPI GSTFSSGSGS



GSSGSGSSGS ASTSTTSTKT TAATSTSTAV AQHYSQCGGQ DWTGPTTCVS PYTCQVQNAY YSQCL


 


SEQ ID NO: 53
MKAYFEYLVA ALPLLGLATA QQVGKQTTET HPKLSWKKCT GKANCNTVNA EVVIDSNWRW LHDSSGKNCY DGNKWTSACS SATDCASKCQ



LDGANYGTTY GASTSGDALT LKFVTKHEYG TNIGSRFYLM NGASKYQMFT LMNNEFAFDV DLSTVECGLN AALYFVAMEE DGGMASYSSN



KAGAKYGTGY CDAQCARDLK FVGGKANIEG WTPSTNDANA GVGPYGGCCA EIDVWESNAH SFAFTPHACK TNKYHVCERD NCGGTYSEDR



FAGLCDANGC DYNPYRMGNT DFYGKGKTVD TSKKFTVVSR FEENKLTQFF VQNGQKIEIP GPKWDGIPSD NANITPEFCS AQFQAFGDRD



RFAEVGGFAQ LNSALRMPMV LVMSIWDDHY ANMLWLDSVY PPEKEGQPGA ARGDCPQSSG VPAEVESQYA NSKVVYSNIR FGPVGSTVNV


 


SEQ ID NO: 54
MFSKFALTGS LLAGAVNAQG VGTQQTETHP QMTWQSCTSP SSCTTNQGEV VIDSNWRWVH DKDGYVNCYT GNTWNTTLCP DDKTCAANCV



LDGADYSSTY GITTSGNALS LQFVTQSSGK NIGSRTYLME SSTKYHLFDL IGNEFAFDVD LSKLPCGLNG ALYFVTMDAD GGMAKYSTNT



AGAEYGTGYC DSQCPRDLKF INGQGNVEGW TPSTNDANAG VGGLGSCCSE MDVWEANSMD MAYTPHPCET AAQHSCNADE CGGTYSSSRY



AGDCDPDGCD WNPFRMGNKD FYGSGDTVDT SQKFTVVTQF HGSGSSLTEI SQYYIQGGTK IQQPNSTWPT LTGYNSITDD FCKAQKVEFN DTDVFSEKGG LAQMGAGMAD



GMVLVMSLWD DHYANMLWLD STYPVDADAS SPGKQRGTCA TTSGVPADVE SSDASATVIY SNIKFGPIGA TY


 


SEQ ID NO: 55
MFPAAALLSF TLLAVASAQQ IGTNTAEVHP SLTVSQCTTS GGCTSSTQSI VLDANWRWLH STSGYTNCYT GNQWNSDLCP DPDTCATNCA



LDGASYESTY GISTDGNAVT LNFVTQGSQT NVGSRVYLLS DDTHYQTFSL LNKEFSFDVD ASNIGCGING AVYFVQMDAD GGLSKYSSNK



AGAQYGTGYC DSQCPQDIKF INGEANLLDW NATSANSGTG SYGSCCPEMD IWEANKYAAA YTPHPCSVSG QTRCTGTSCG AGSERYDGYC



DKDGCDFNSW RMGNETFLGP GMTIDTNKKF TIVTQFITDD NTANGTLSEI RRLYVQGGTV IQNSVANQPN IPKVNSITDS FCTAQKTEFG



DQDYFGTIGG LSQMGKAMSD MVLVMSIWDD YDAEMLWLDS NYPTSGSAST PGISRGPCSA TSGLPATVES QQASASVTYS NIKWGDIGST



YSGSGSSGSS SSSSSSAASA STSTHTSAAA TATSSAAAAT GSPVPAYGQC GGQSYTGSTT CASPYVCKVS NAYYSQCLPA


 


SEQ ID NO: 56
MKRALCASLS LLAAAVAQQV GTNEPEVHPK MTWKKCSSGG SCSTVNGEVV IDGNWRWIHN IGGYENCYSG NKWTSVCSTN ADCATKCAME



GAKYQETYGV STSGDALTLK FVQQNSSGKN VGSRMYLMNG ANKYQMFTLK NNEFAFDVDL SSVECGMNSA LYFVPMKEDG GMSTEPNNKA



GAKYGTGYCD AQCARDLKFI GGKGNIEGWQ PSSTDSSAGI GAQGACCAEI DIWESNKNAF AFTPHPCENN EYHVCTEPNC GGTYADDRYG



GGCDANGCDY NPYRMGNPDF YGPGKTIDTN RKFTVISRFE NNRNYQILMQ DGVAHRIPGP KFDGLEGETG ELNEQFCTDQ FTVFDERNRF



NEVGGWSKLN AAYEIPMVLV MSIWSDHFAN MLWLDSTYPP EKAGQPGSAR GPCPADGGDP NGVVNQYPNA KVIWSNVRFG PIGSTYQVD


 


SEQ ID NO: 57
MQLTKAGVFL GALMGGAAAQ QVGTQTAENH PKMTWKKCTG KASCTTVNGE VVIDANWRWL HDASSKNCYD GNRWTDSCRT ASDCAAKCSL



EGADYAKTYG ASTSGDALSL KFVTRHDYGT NIGSRFYLMN GASKYQMFSL LGNEFAFDVD LSTIECGLNS ALYFVAMEED GGMKSYSSNK



AGAKYGTGYC DAQCARDLKF VGGKANIEGW KPSSNDANAG VGPYGACCAE IDVWESNAHA FAFTPHPCTD NKYHVCQDSN CGGTYSDDRF



AGKCDANGCD INPYRLGNTD FYGKGKTVDT SKKFTVVTRF ERDALTQFFV QNNKRIDMPS PALEGLPATG AITAEYCTNV FNVFGDRNRF DEVGGWSQLQ QALSLPMVLV



MSIWDDHYSN MLWLDSVYPP DKEGSPGAAR GDCPQDSGVP SEVESQIPGA TVVWSNIRFG PVGSTVNV


 


SEQ ID NO: 58
MYRIVATASA LIAAARAQQV CSLNTETKPA LTWSKCTSSG CSDVKGSVVI DANWRWTHQT SGSTNCYTGN KWDTSICTDG KTCAEKCCLD



GADYSGTYGI TSSGNQLSLG FVTNGPYSKN IGSRTYLMEN ENTYQMFQLL GNEFTFDVDV SGIGCGLNGA PHFVSMDEDG GKAKYSGNKA



GAKYGTGYCD AQCPRDVKFI NGVANSEGWK PSDSDVNAGV GNLGTCCPEM DIWEANSIST AFTPHPCTKL TQHSCTGDSC GGTYSSDRYG



GTCDADGCDF NAYRQGNKTF YGPGSNFNID TTKKMTVVTQ FHKGSNGRLS EITRLYVQNG KVIANSESKI AGNPGSSLTS DFCSKQKSVF



GDIDDFSKKG GWNGMSDALS APMVLVMSLW HDHHSNMLWL DSTYPTDSTK VGSQRGSCAT TSGKPSDLER DVPNSKVSFS NIKFGPIGST



YKSDGTTPNP PASSSTTGSS TPTNPPAGSV DQWGQCGGQN YSGPTTCKSP FTCKKINDFY SQCQ


 


SEQ ID NO: 59
MYQRALLFSA LATAVSAQQV GTQKAEVHPA LTWQKCTAAG SCTDQKGSVV IDANWRWLHS TEDTTNCYTG NEWNAELCPD NEACAKNCAL



DGADYSGTYG VTADGSSLKL NFVTSANVGS RLYLMEDDET YQMFNLLNNE FTFDVDVSNL PCGLNGALYF VSMDADGGLS KYPGNKAGAK



YGTGYCDSQC PRDLKFINGE ANVEGWKPSD NDKNAGVGGY GSCCPEMDIW EANSISTAYT PHPCDGMEQT RCDGNDCGGT YSSTRYAGTC



DPDGCDFNSF RMGNESFYGP GGLVDTKSPI TVVTQFVTAG GTDSGALKEI RRVYVQGGKV IGNSASNVAG VEGDSITSDF CTAQKKAFGD



EDIFSKHGGL EGMGKALNKM ALIVSIWDDH ASSMMWLDST YPVDADASTP GVARGTCEHG LGDPETVESQ HPDASVTFSN IKFGPIGSTY KSV


 


SEQ ID NO: 60
MSALNSFNMY KSALILGSLL ATAGAQQIGT YTAETHPSLS WSTCKSGGSC TTNSGAITLD ANWRWVHGVN TSTNCYTGNT WNTAICDTDA



SCAQDCALDG ADYSGTYGIT TSGNSLRLNF VTGSNVGSRT YLMADNTHYQ IFDLLNQEFT FTVDVSNLPC GLNGALYFVT MDADGGVSKY



PNNKAGAQYG VGYCDSQCPR DLKFIAGQAN VEGWTPSTNN SNTGIGNHGS CCAELDIWEA NSISEALTPH PCDTPGLTVC TADDCGGTYS



SNRYAGTCDP DGCDFNPYRL GVTDFYGSGK TVDTTKPFTV VTQFVTDDGT SSGSLSEIRR YYVQNGVVIP QPSSKISGIS GNVINSDFCA



AELSAFGETA SFTNHGGLKN MGSALEAGMV LVMSLWDDYS VNMLWLDSTY PANETGTPGA ARGSCPTTSG NPKTVESQSG SSYVVFSDIK VGPFNSTFSG GTSTGGSTTT



TASGTTSTKA STTSTSSTST GTGVAAHWGQ CGGQGWTGPT TCASGTTCTV VNPYYSQCL


 


SEQ ID NO: 61
MRTAKFATLA ALVASAAAQQ ACSLTTERHP SLSWNKCTAG GQCQTVQASI TLDSNWRWTH QVSGSTNCYT GNKWDTSICT DAKSCAQNCC



VDGADYTSTY GITTNGDSLS LKFVTKGQHS TNVGSRTYLM DGEDKYQTFE LLGNEFTFDV DVSNIGCGLN GALYFVSMDA DGGLSRYPGN



KAGAKYGTGY CDAQCPRDIK FINGEANIEG WTGSTNDPNA GAGRYGTCCS EMDIWEANNM ATAFTPHPCT IIGQSRCEGD SCGGTYSNER



YAGVCDPDGC DFNSYRQGNK TFYGKGMTVD TTKKITVVTQ FLKDANGDLG EIKRFYVQDG KIIPNSESTI PGVEGNSITQ DWCDRQKVAF



GDIDDFNRKG GMKQMGKALA GPMVLVMSIW DDHASNMLWL DSTFPVDAAG KPGAERGACP TTSGVPAEVE AEAPNSNVVF SNIRFGPIGS



TVAGLPGAGN GGNNGGNPPP PTTTTSSAPA TTTTASAGPK AGRWQQCGGI GFTGPTQCEE PYICTKLNDW YSQCL


 


SEQ ID NO: 62
MMYKKFAALA ALVAGASAQQ ACSLTAENHP SLTWKRCTSG GSCSTVNGAV TIDANWRWTH TVSGSTNCYT GNQWDTSLCT DGKSCAQTCC



VDGADYSSTY GITTSGDSLN LKFVTKHQYG TNVGSRVYLM ENDTKYQMFE LLGNEFTFDV DVSNLGCGLN GALYFVSMDA DGGMSKYSGN



KAGAKYGTGY CDAQCPRDLK FINGEANVGN WTPSTNDANA GFGRYGSCCS EMDVWEANNM ATAFTPHPCT TVGQSRCEAD TCGGTYSSDR



YAGVCDPDGC DFNAYRQGDK TFYGKGMTVD TNKKMTVVTQ FHKNSAGVLS EIKRFYVQDG KIIANAESKI PGNPGNSITQ EYCDAQKVAF



SNTDDFNRKG GMAQMSKALA GPMVLVMSVW DDHYANMLWL DSTYPIDQAG APGAERGACP TTSGVPAEIE AQVPNSNVIF SNIRFGPIGS



TVPGLDGSNP GNPTTTVVPP ASTSTSRPTS STSSPVSTPT GQPGGCTTQK WGQCGGIGYT GCTNCVAGTT CTQLNPWYSQ CL


 


SEQ ID NO: 63
MASLSLSKIC RNALILSSVL STAQGQQVGT YQTETHPSMT WQTCGNGGSC STNQGSVVLD ANWRWVHQTG SSSNCYTGNK WDTSYCSTND



ACAQKCALDG ADYSNTYGIT TSGSEVRLNF VTSNSNGKNV GSRVYMMADD THYEVYKLLN QEFTFDVDVS KLPCGLNGAL YFVVMDADGG



VSKYPNNKAG AKYGTGYCDS QCPRDLKFIQ GQANVEGWVS STNNANTGTG NHGSCCAELD IWESNSISQA LTPHPCDTPT NTLCTGDACG



GTYSSDRYSG TCDPDGCDFN PYRVGNTTFY GPGKTIDTNK PITVVTQFIT DDGTSSGTLS EIKRFYVQDG VTYPQPSADV SGLSGNTINS EYCTAENTLF EGSGSFAKHG



GLAGMGEAMS TGMVLVMSLW DDYYANMLWL DSNYPTNEST SKPGVARGTC STSSGVPSEV EASNPSAYVA YSNIKVGPIG STFKS


 


SEQ ID NO: 64
MYRAIATASA LIAAVRAQQV CSLTPETKPA LSWSKCTSSG CSNVQGSVTI DANWRWTHQL SGSTNCYTGN KWDTSICTSG KVCAEKCCID



GAEYASTYGI TSSGNQLSLS FVTKGAYGTN IGSRTYLMED ENTYQMFQLL GNEFTFDVDV SNIGCGLNGA LYFVSMDADG GKAKYPGNKA



GAKYGTGYCD AQCPRDVKFI NGQANSDGWQ PSKSDVNAGI GNMGTCCPEM DIWEANSIST AYTPHPCTKL TQHSCTGDSC GGTYSNDRYG



GTCDADGCDF NAYRQGNKTF YGPGSGFNVD TTKKVTVVTQ FHKGSNGRLS EITRLYVQNG KVIANSESKI AGVPGSSLTP EFCTAQKKVF



GDTDDFAKKG AWSGMSDALE APMVLVMSLW HDHHSNMLWL DSTYPTDSTK LGAQRGSCST SSGVPADLEK NVPNSKVAFS NIKFGPIGST



YKEGVPEPTN PTNPTNPTNP TNPGTVDQWA QCGGTNYSGP TACKSPFTCK KINDFYSQCQ


 


SEQ ID NO: 65
MFPKSSLLVL SFLATAYAQQ VGTQTAEVHP SLNWARCTSS GCTNVAGSVT LDANWRWLHT TSGYTNCYTG NSWNTTLCPD GATCAQNCAL



DGANYQSTCG ITTSGNALTL KFVTQGEQKN IGSRVYLMAS ESRYEMFGLL NKEFTFDVDV SNLPCGLNGA LYFSSMDADG GMAKNPGNKA



GAKYGTGYCD SQCPRDIKFI NGEANVAGWN GSPNDTNAGT GNWGACCNEM DIWEANSISA AYTPHPCTVQ GLSRCSGTAC GTNDRYGTVC



DPDGCDFNSY RMGDKTYYGP GGTGVDTRSK FTVVTQFLTN NNSSSGTLSE IRRLYVQNGR VVQNSKVNIP GMSNTLDSIT TGFCDSQKTA



FGDTRSFQNK GGMSAMGQAL GAGMVLVLSV WDDHAANMLW LDSNYPVDAD PSKPGIARGT CSTTSGKPTD VEQSAANSSV TFSNIKFGDI



GTTYTGGSVT TTPGNPGTTT STAPGAVQTK WGQCGGQGWT GPTRCESGST CTVVNQWYSQ CI


 


SEQ ID NO: 66
MFRKAALLAF SFLAIAHGQQ VGTNQAENHP SLPSQHCTAS GCTTSSTSVV LDANWRWVHT TTGYTNCYTG QTWDASICPD GVTCAKACAL



DGADYSGTYG ITTSGNALTL QFVKGTNVGS RVYLLQDASN YQLFKLINQE FTFDVDMSNL PCGLNGAVYL SQMDQDGGVS RFPTNTAGAK



YGTGYCDSQC PRDIKFINGE ANVAGWTGSS SDPNSGTGNY GTCCSEMDIW EANSVAAAYT PHPCSVNQQT RCTGADCGQD ANRYKGVCDP



DGCDFNSFRM GDQTFLGKGL TVDTSRKFTI VTQFISDDGT SSGNLAEIRR FYVQDGKVIP NSKVNIAGCD AVNSITDKFC TQQKTAFGDT



NRFADQGGLK QMGAALKSGM VLALSLWDDH AANMLWLDSD YPTTADASKP GVARGTCPNT SGVPKDVESQ SGSATVTYSN IKWGDLNSTF



SGTASNPTGP SSSPSGPSSS SSSTAGSQPT QPSSGSVAQW GQCGGIGYSG ATGCVSPYTC HVVNPYYSQC Y


 


SEQ ID NO: 67
TETHPRLTWK RCTSGGNCST VNGAVTIDAN WRWTHTVSGS TNCYTGNEWD TSICSDGKSC AQTCCVDGAD YSSTYGITTS GDSLNLKFVT



KHQHGTNVGS RVYLMENDTK YQMFELLGNE FTFDVDVSNL GCGLNGALYF VSMDADGGMS KYSGNKAGAK YGTGYCDAQC PRDLKFINGE



ANIENWTPST NDANAGFGRY GSCCSEMDIW EANNMATAFT PHPCTIIGQS RCEGNSCGGT YSSERYAGVC DPDGCDFNAY RQGDKTFYGK



GMTVDTTKKM TVVTQFHKNS AGVLSEIKRF YVQDGKIIAN AESKIPGNPG NSITQEWCDA QKVAFGDIDD FNRKGGMAQM SKALEGPMVL



VMSVWDDHYA NMLWLDSTYP IDKAGTPGAE RGACPTTSGV PAEIEAQVPN SNVIFSNIRF GPIGSTVPGL DGSTPSNPTA TVAPPTSTTT



SVRSSTTQIS TPTSQPGGCT TQKWGQCGGI GYTGCTNCVA GTTCTELNPW YSQCL


 


SEQ ID NO: 68
MFHKAVLVAF SLVTIVHGQQ AGTQTAENHP QLSSQKCTAG GSCTSASTSV VLDSNWRWVH TTSGYTNCYT GNTWDASICS DPVSCAQNCA



LDGADYAGTY GITTSGDALT LKFVTGSNVG SRVYLMEDET NYQMFKLMNQ EFTFDVDVSN LPCGLNGAVY FVQMDQDGGT SKFPNNKAGA



KFGTGYCDSQ CPQDIKFING EANIVDWTAS AGDANSGTGS FGTCCQEMDI WEANSISAAY TPHPCTVTEQ TRCSGSDCGQ GSDRFNGICD



PDGCDFNSFR MGNTEFYGKG LTVDTSQKFT IVTQFISDDG TADGNLAEIR RFYVQNGKVI PNSVVQITGI DPVNSITEDF CTQQKTVFGD



TNNFAAKGGL KQMGEAVKNG MVLALSLWDD YAAQMLWLDS DYPTTADPSQ PGVARGTCPT TSGVPSQVEG QEGSSSVIYS NIKFGDLNST FTGTLTNPSS PAGPPVTSSP



SEPSQSTQPS QPAQPTQPAG TAAQWAQCGG MGFTGPTVCA SPFTCHVLNP YYSQCY


 


SEQ ID NO: 69
MFRAAALLAF TCLAMVSGQQ AGTNTAENHP QLQSQQCTTS GGCKPLSTKV VLDSNWRWVH STSGYTNCYT GNEWNTSLCP DGKTCAANCA



LDGADYSGTY GITSTGTALT LKFVTGSNVG SRVYLMADDT HYQLLKLLNQ EFTFDVDMSN LPCGLNGALY LSAMDADGGM SKYPGNKAGA



KYGTGYCDSQ CPKDIKFING EANVGNWTET GSNTGTGSYG TCCSEMDIWE ANNDAAAFTP HPCTTTGQTR CSGDDCARNT GLCDHGDGCD



FNSFRMGDKT FLGKGMTVDT SKPFTDVTQF LTNDNTSTGT LSEIRRIYIQ NGKVIQNSVA NIPGVDPVNS ITDNFCAQQK TAFGDTNWFA



QKGGLKQMGE ALGNGMVLAL SIWDDHAANM LWLDSDYPTD KDPSAPGVAR GTCATTSGVP SDVESQVPNS QVVFSNIKFG DIGSTFSGTS



SPNPPGGSTT SSPVTTSPTP PPTGPTVPQW GQCGGIGYSG STTCASPYTC HVLNPYYSQC Y


 


SEQ ID NO: 70
MMMKQYLQYL AAALPLVGLA AGQRAGNETP ENHPPLTWQR CTAPGNCQTV NAEVVIDANW RWLHDDNMQN CYDGNQWTNA CSTATDCAEK



CMIEGAGDYL GTYGASTSGD ALTLKFVTKH EYGTNVGSRF YLMNGPDKYQ MFNLMGNELA FDVDLSTVEC GINSALYFVA MEEDGGMASY



PSNQAGARYG TGYCDAQCAR DLKFVGGKAN IEGWKSSTSD PNAGVGPYGS CCAEIDVWES NAYAFAFTPH ACTTNEYHVC ETTNCGGTYS



EDRFAGKCDA NGCDYNPYRM GNPDFYGKGK TLDTSRKFTV VSRFEENKLS QYFIQDGRKI EIPPPTWEGM PNSSEITPEL CSTMFDVFND RNRFEEVGGF EQLNNALRVP



MVLVMSIWDD HYANMLWLDS IYPPEKEGQP GAARGDCPTD SGVPAEVEAQ FPDAQVVWSN IRFGPIGSTY DF


 


SEQ ID NO: 71
MYRSATFLTF ASLVLGQQVG TYTAERHPSM PIQVCTAPGQ CTRESTEVVL DANWRWTHIT NGYTNCYTGN EWNATACPDG ATCAKNCAVD



GADYSGTYGI TTPSSGALRL QFVKKNDNGQ NVGSRVYLMA SSDKYKLFNL LNKEFTFDVD VSKLPCGLNG AVYFSEMLED GGLKSFSGNK



AGAKYGTGYC DSQCPQDIKF INGEANVEGW GGADGNSGTG KYGICCAEMD IWEANSDATA YTPHVCSVNE QTRCEGVDCG AGSDRYNSIC



DKDGCDFNSY RLGNREFYGP GKTVDTTRPF TIVTQFVTDD GTDSGNLKSI HRYYVQDGNV IPNSVTEVAG VDQTNFISEG FCEQQKSAFG



DNNYFGQLGG MRAMGESLKK MVLVLSIWDD HAVNMNWLDS IFPNDADPEQ PGVARGRCDP ADGVPATIEA AHPDAYVIYS NIKFGAINST FTAN


 


SEQ ID NO: 72
MYRTLAFASL SLYGAARAQQ VGTSTAENHP KLTWQTCTGT GGTNCSNKSG SVVLDSNWRW AHNVGGYTNC YTGNSWSTQY CPDGDSCTKN



CAIDGADYSG TYGITTSNNA LSLKFVTKGS FSSNIGSRTY LMETDTKYQM FNLINKEFTF DVDVSKLPCG LNGALYFVEM AADGGIGKGN



NKAGAKYGTG YCDSQCPHDI KFINGKANVE GWNPSDADPN GGAGKIGACC PEMDIWEANS ISTAYTPHPC RGVGLQECSD AASCGDGSNR



YDGQCDKDGC DFNSYRMGVK DFYGPGATLD TTKKMTVITQ FLGSGSSLSE IKRFYVQNGK VYKNSQSAVA GVTGNSITES FCTAQKKAFG



DTSSFAALGG LNEMGASLAR GHVLIMSLWG DHAVNMLWLD STYPTDADPS KPGAARGTCP TTSGKPEDVE KNSPDATVVF SNIKFGPIGS TFAQPA


 


SEQ ID NO: 73
MYQKLALISA FLATARAQSA CTLQAETHPP LTWQKCSSGG TCTQQTGSVV IDANWRWTHA TNSSTNCYDG NTWSSTLCPD NETCAKNCCL



DGAAYASTYG VTTSADSLSI GFVTQSAQKN VGARLYLMAS DTTYQEFTLL GNEFSFDVDV SQLPCGLNGA LYFVSMDADG GVSKYPTNTA



GAKYGTGYCD SQCPRDLKFI NGQANVEGWE PSSNNANTGI GGHGSCCSEM DIWEANSISE ALTPHPCTTV GQEICDGDSC GGTYSGDRYG



GTCDPDGCDW NPYRLGNTSF YGPGSSFTLD TTKKLTVVTQ FETSGAINRY YVQNGVTFQQ PNAELGDYSG NSLDDDYCAA EEAEFGGSSF



SDKGGLTQFK KATSGGMVLV MSLWDDYYAN MLWLDSTYPT NETSSTPGAV RGSCSTSSGV PAQLESNSPN AKVVYSNIKF GPIGSTGNSS



GGNPPGGNPP GTTTTRRPAT STGSSPGPTQ THYGQCGGIG YSGPTVCASG STCQVLNPYY SQCL


 


SEQ ID NO: 74
MVDSFSIYKT ALLLSMLATS NAQQVGTYTA ETHPSLTWQT CSGSGSCTTT SGSVVIDANW RWVHEVGGYT NCYSGNTWDS SICSTDTTCA



SECALEGATY ESTYGVTTSG SSLRLNFVTT ASQKNIGSRL YLLADDSTYE TFKLFNREFT FDVDVSNLPC GLNGALYFVS MDADGGVSRF



PTNKAGAKYG TGYCDSQCPR DLKFIDGQAN IEGWEPSSTD VNAGTGNHGS CCPEMDIWEA NSISSAFTAH PCDSVQQTMC TGDTCGGTYS



DTTDRYSGTC DPDGCDFNPY RFGNTNFYGP GKTVDNSKPF TVVTQFITHD GTDTGTLTEI RRLYVQNGVV IGNGPSTYTA ASGNSITESF



CKAEKTLFGD TNVFETHGGL SAMGDALGDG MVLVLSLWDD HAADMLWLDS DYPTTSCASS PGVARGTCPT TTGNATYVEA NYPNSYVTYS NIKFGTLNST YSGTSSGGSS



SSSTTLTTKA STSTTSSKTT TTTSKTSTTS SSSTNVAQLY GQCGGQGWTG PTTCASGTCTKQNDYYSQCL


 


SEQ ID NO: 75
MYRILKSFIL LSLVNMSLSQ KIGKLTPEVH PPMTFQKCSE GGSCETIQGE VVVDANWRWV HSAQGQNCYT GNTWNPTICP DDETCAENCY



LDGANYESVY GVTTSEDSVR LNFVTQSQGK NIGSRLFLMS NESNYQLFHV LGQEFTFDVD VSNLDCGLNG ALYLVSMDSD GGSARFPTNE



AGAKYGTGYC DAQCPRDLKF ISGSANVDGW IPSTNNPNTG YGNLGSCCAE MDLWEANNMA TAVTPHPCDT SSQSVCKSDS CGGAASSNRY



GGICDPDGCD YNPYRMGNTS FFGPNKMIDT NSVITVVTQF ITDDGSSDGK LTSIKRLYVQ DGNVISQSVS TIDGVEGNEV NEEFCTNQKK



VFGDEDSFTK HGGLAKMGEA LKDGMVLVLS LWDDYQANML WLDSSYPTTS SPTDPGVARG SCPTTSGVPS KVEQNYPNAY VVYSNIKVGP IDSTYKK


 


SEQ ID NO: 76
MISRVLAISS LLAAARAQQI GTNTAEVHPA LTSIVIDANW RWLHTTSGYT NCYTGNSWDA TLCPDAVTCA ANCALDGADY SGTYGITTSG



NSLKLNFVTK GANTNVGSRT YLMAAGSKTQ YQLLKLLGQE FTFDVDVSNL PCGLNGALYF AEMDADGGVS RFPTNKAGAQ YGTGYCDAQC



PQDIKFINGQ ANSVGWTPSS NDVNTGTGQY GSCCSEMDIW EANKISAAYT PHPCSVDGQT RCTGTDCGIG ARYSSLCDAD GCDFNSYRMG



DTGFYGAGLT VDTSKVFTVV TQFITNDGTT SGTLSEIRRF YVQNGKVIPN SQSKVTGVSG NSITDSFCAA QKTAFGDTNE FATKGGLATM



SKALAKGMVL VMSIWDDHSA NMLWLDAPYP ASKSPSAAGV SRGSCSASSG VPADVEANSP GASVTYSNIK WGPINSTYSA GTGSNTGSGS



GSTTTLVSSV PSSTPTSTTG VPKYGQCGGS GYTGPTNCIG STCVSMGQYY SQCQ


 


SEQ ID NO: 77
MYRQVATALS FASLVLGQQV GTLTAETHPS LPIEVCTAPG SCTKEDTTVV LDANWRWTHV TDGYTNCYTG NAWNETACPD GKTCAANCAI



DGAEYEKTYG ITTPEEGALR LNFVTESNVG SRVYLMAGED KYRLFNLLNK EFTMDVDVSN LPCGLNGAVY FSEMDEDGGM SRFEGNKAGA



KYGTGYCDSQ CPRDIKFING EANSEGWGGE DGNSGTGKYG TCCAEMDIWE ANLDATAYTP HPCKVTEQTR CEDDTECGAG DARYEGLCDR



DGCDFNSFRL GNKEFYGPEK TVDTSKPFTL VTQFVTADGT DTGALQSIRR FYVQDGTVIP NSETVVEGVD PTNEITDDFC AQQKTAFGDN



NHFKTIGGLP AMGKSLEKMV LVLSIWDDHA VYMNWLDSNY PTDADPTKPG VARGRCDPEA GVPETVEAAH PDAYVIYSNI KIGALNSTFA AA


 


SEQ ID NO: 78
MSSFQVYRAA LLLSILATAN AQQVGTYTTE THPSLTWQTC TSDGSCTTND GEVVIDANWR WVHSTSSATN CYTGNEWDTS ICTDDVTCAA



NCALDGATYE ATYGVTTSGS ELRLNFVTQG SSKNIGSRLY LMSDDSNYEL FKLLGQEFTF DVDVSNLPCG LNGALYFVAM DADGGTSEYS



GNKAGAKYGT GYCDSQCPRD LKFINGEANC DGWEPSSNNV NTGVGDHGSC CAEMDVWEAN SISNAFTAHP CDSVSQTMCD GDSCGGTYSA



SGDRYSGTCD PDGCDYNPYR LGNTDFYGPG LTVDTNSPFT VVTQFITDDG TSSGTLTEIK RLYVQNGEVI ANGASTYSSV NGSSITSAFC



ESEKTLFGDE NVFDKHGGLE GMGEAMAKGM VLVLSLWDDY AADMLWLDSD YPVNSSASTP GVARGTCSTD SGVPATVEAE SPNAYVTYSN IKFGPIGSTY SSGSSSGSGS



SSSSSSTTTK ATSTTLKTTS TTSSGSSSTS AAQAYGQCGG QGWTGPTTCV SGYTCTYENA YYSQCL


 


SEQ ID NO: 79
MYRAIATASA LLATARAQQV CTLNTENKPA LTWAKCTSSG CSNVRGSVVV DANWRWAHST SSSTNCYTGN TWDKTLCPDG KTCADKCCLD



GADYSGTYGV TSSGNQLNLK FVTVGPYSTN VGSRLYLMED ENNYQMFDLL GNEFTFDVDV NNIGCGLNGA LYFVSMDKDG GKSRFSTNKA



GAKYGTGYCD AQCPRDVKFI NGVANSDEWK PSDSDKNAGV GKYGTCCPEM DIWEANKIST AYTPHPCKSL TQQSCEGDAC GGTYSATRYA



GTCDPDGCDF NPYRQGNKTF YGPGSGFNVD TTKKVTVVTQ FIKGSDGKLS EIKRLYVQNG KVIGNPQSEI ANNPGSSVTD SFCKAQKVAF



NDPDDFNKKG GWSGMSDALA KPMVLVMSLW HDHYANMLWL DSTYPKGSKT PGSARGSCPE DSGDPDTLEK EVPNSGVSFS NIKFGPIGST



YTGTGGSNPD PEEPEEPEEP VGTVPQYGQC GGINYSGPTA CVSPYKCNKI NDFYSQCQ


 


SEQ ID NO: 80
EQAGTATAEN HPPLTWQECT APGSCTTQNG AVVLDANWRW VHDVNGYTNC YTGNTWDPTY CPDDETCAQN CALDGADYEG TYGVTSSGSS



LKLNFVTGSN VGSRLYLLQD DSTYQIFKLL NREFSFDVDV SNLPCGLNGA LYFVAMDADG GVSKYPNNKA GAKYGTGYCD SQCPRDLKFI



DGEANVEGWQ PSSNNANTGI GDHGSCCAEM DVWEANSISN AVTPHPCDTP GQTMCSGDDC GGTYSNDRYA GTCDPDGCDF NPYRMGNTSF



YGPGKIIDTT KPFTVVTQFL TDDGTDTGTL SEIKRFYIQN SNVIPQPNSD ISGVTGNSIT TEFCTAQKQA FGDTDDFSQH GGLAKMGAAM



QQGMVLVMSL WDDYAAQMLW LDSDYPTDAD PTTPGIARGT CPTDSGVPSD VESQSPNSYV TYSNIKFGPI NSTFTAS


 


SEQ ID NO: 81
MFPTLALVSL SFLAIAYGQQ VGTLTAETHP KLSVSQCTAG GSCTTVQRSV VLDSNWRWLH DVGGSTNCYT GNTWDDSLCP DPTTCAANCA



LDGADYSGTY GITTSGNALS LKFVTQGPYS TNIGSRVYLL SEDDSTYEMF NLKNQEFTFD VDMSALPCGL NGALYFVEMD KDGGSGRFPT



NKAGSKYGTG YCDTQCPHDI KFINGEANVL DWAGSSNDPN AGTGHYGTCC NEMDIWEANS MGAAVTPHVC TVQGQTRCEG TDCGDGDERY



DGICDKDGCD FNSWRMGDQT FLGPGKTVDT SSKFTVVTQF ITADNTTSGD LSEIRRLYVQ NGKVIANSKT QIAGMDAYDS ITDDFCNAQK



TTFGDTNTFE QMGGLATMGD AFETGMVLVM SIWDDHEAKM LWLDSDYPTD ADASAPGVSR GPCPTTSGDP TDVESQSPGA TVIFSNIKTG PIGSTFTS


 


SEQ ID NO: 82
MLSASKAAAI LAFCAHTASA WVVGDQQTET HPKLNWQRCT GKGRSSCTNV NGEVVIDANW RWLAHRSGYT NCYTGSEWNQ SACPNNEACT



KNCAIEGSDY AGTYGITTSG NQMNIKFITK RPYSTNIGAR TYLMKDEQNY EMFQLIGNEF TFDVDLSQRC GMNGALYFVS MPQKGQGAPG



AKYGTGYCDA QCARDLKFVR GSANAEGWTK SASDPNSGVG KKGACCAQMD VWEANSAATA LTPHSCQPAG YSVCEDTNCG GTYSEDRYAG



TCDANGCDFN PFRVGVKDFY GKGKTVDTTK KMTVVTQFVG SGNQLSEIKR FYVQDGKVIA NPEPTIPGME WCNTQKKVFQ EEAYPFNEFG



GMASMSEGMS QGMVLVMSLW DDHYANMLWL DSNWPREADP AKPGVARRDC PTSGGKPSEV EAANPNAQVM FSNIKFGPIG STFAHAA


 


SEQ ID NO: 83
MFRTATLLAF TMAAMVFGQQ VGTNTARSHP ALTSQKCTKS GGCSNLNTKI VLDANWRWLH STSGYTNCYT GNQWDATLCP DGKTCAANCA



LDGADYTGTY GITASGSSLK LQFVTGSNVG SRVYLMADDT HYQMFQLLNQ EFTFDVDMSN LPCGLNGALY LSAMDADGGM AKYPTNKAGA



KYGTGYCDSQ CPRDIKFING EANVEGWNAT SANAGTGNYG TCCTEMDIWE ANNDAAAYTP HPCTTNAQTR CSGSDCTRDT GLCDADGCDF



NSFRMGDQTF LGKGLTVDTS KPFTVVTQFI TNDGTSAGTL TEIRRLYVQN GKVIQNSSVK IPGIDPVNSI TDNFCSQQKT AFGDTNYFAQ



HGGLKQVGEA LRTGMVLALS IWDDYAANML WLDSNYPTNK DPSTPGVARG TCATTSGVPA QIEAQSPNAY VVFSNIKFGD LNTTYTGTVS



SSSVSSSHSS TSTSSSHSSS STPPTQPTGV TVPQWGQCGG IGYTGSTTCA SPYTCHVLNP YYSQCY


 


SEQ ID NO: 84
MYQRALLFSA LMAGVSAQQV GTQKPETHPP LAWKECTSSG CTSKDGSVVI DANWRWVHSV DGYKNCYTGN EWDSTLCPDD ATCATNCAVD



GADYAGTYGA TTEGDSLSIN FVTGSNIGSR FYLMEDENKY QMFKLLNKEF TFDVDVSTLP CGLNGALYFV SMDADGGMSK YETNKAGAKY



GTGYCDSQCP RDLKFINGKG NVEGWKPSAN DKNAGVGPHG SCCAEMDIWE ANSISTALTP HPCDTNGQTI CEGDSCGGTY STTRYAGTCD



PDGCDFNPFR MGNESFYGPG KMVDTKSKMT VVTQFITSDG TDTGSLKEIK RVYVQNGKVI ANSASDVSGI TGNSITSDFC TAQKKTFGDE DVFNKHGGLS GMGDALGEGM



VLVMSLWDDH NSNMLWLDGE KYPTDAAASK AGVSRGTCST DSGKPSTVES ESGSAKVVFS NIKVGSIGST FSA


 


SEQ ID NO: 85
MTSKIALASL FAAAYGQQIG TYTTETHPSL TWQSCTAKGS CTTQSGSIVL DGNWRWTHST TSSTNCYTGN TWDATLCPDD ATCAQNCALD



GADYSGTYGI TTSGDSLRLN FVTQTANKNV GSRVYLLADN THYKTFNLLN QEFTFDVDVS NLPCGLNGAV YFANLPADGG ISSTNKAGAQ



YGTGYCDSQC PRDGKFINGK ANVDGWVPSS NNPNTGVGNY GSCCAEMDIW EANSISTAVT PHSCDTVTQT VCTGDNCGGT YSTTRYAGTC



DPDGCDFNPY RQGNESFYGP GKTVDTNSVF TIVTQFLTTD GTSSGTLNEI KRFYVQNGKV IPNSESTISG VTGNSITTPF CTAQKTAFGD



PTSFSDHGGL ASMSAAFEAG MVLVLSLWDD YYANMLWLDS TYPTTKTGAG GPRGTCSTSS GVPASVEASS PNAYVVYSNI KVGAINSTFG


 


SEQ ID NO: 86
MYTKFAALAA LVATVRGQAA CSLTAETHPS LQWQKCTAPG SCTTVSGQVT IDANWRWLHQ TNSSTNCYTG NEWDTSICSS DTDCATKCCL



DGADYTGTYG VTASGNSLNL KFVTQGPYSK NIGSRMYLME SESKYQGFTL LGQEFTFDVD VSNLGCGLNG ALYFVSMDLD GGVSKYTTNK



AGAKYGTGYC DSQCPRDLKF INGQANIDGW QPSSNDANAG LGNHGSCCSE MDIWEANKVS AAYTPHPCTT IGQTMCTGDD CGGTYSSDRY



AGICDPDGCD FNSYRMGDTS FYGPGKTVDT GSKFTVVTQF LTGSDGNLSE IKRFYVQNGK VIPNSESKIA GVSGNSITTD FCTAQKTAFG



DTNVFEERGG LAQMGKALAE PMVLVLSVWD DHAVNMLWLD STYPTDSTKP GAARGDCPIT SGVPADVESQ APNSNVIYSN IRFGPINSTY



TGTPSGGNPP GGGTTTTTTT TTSKPSGPTT TTNPSGPQQT HWGQCGGQGW TGPTVCQSPY TCKYSNDWYS QCL


 


SEQ ID NO: 87
MYQRALLFSA LLSVSRAQQA GTAQEEVHPS LTWQRCEASG SCTEVAGSVV LDSNWRWTHS VDGYTNCYTG NEWDATLCPD NESCAQNCAV



DGADYEATYG ITSNGDSLTL KFVTGSNVGS RVYLMEDDET YQMFDLLNNE FTFDVDVSNF PCGLNGALYF TSMDADGGLS KYEGNTAGAK



YGTGYCDSQC PRDIKFINGL GNVEGWEPSD SDANAGVGGM GTCCPEMDIW EANSISTAYT PHPCDSVEQT MCEGDSCGGT YSDDRYGGTC



DPDGCDFNSY RMGNTRFYGP GAIIDTSSKF TVVTQFIADG GSLSEIKRFY VQNGEVIPNS ESNISGVEGN SITSEFCTAQ KTAFGDEDIF



AQHGGLSAMG DAASAMVLIL SIWDDHHSSM MWLDSSYPTD ADPSQPGVAR GTCEQGAGDP DVVESEHADA SVTFSNIKFG PIGSTF


 


SEQ ID NO: 88
MMMKQYLQYL AAGSLMTGLV AGQGVGTQQT ETHPRITWKR CTGKANCTTV QAEVVIDSNW RWIHTSGGTN CYDGNAWNTA ACSTATDCAS



KCLMEGAGNY QQTYGASTSG DSLTLKFVTK HEYGTNVGSR FYLMNGASKY QMFTLMNNEF TFDVDLSTVE CGLNSALYFV AMEEDGGMRS



YPTNKAGAKY GTGYCDAQCA RDLKFVGGKA NIEGWRESSN DENAGVGPYG GCCAEIDVWE SNAHAYAFTP HACENNNYHV CERDTCGGTY



SEDRFAGGCD ANGCDYNPYR MGNPDFYGKG KTVDTTKKFT VVTRFQDDNL EQFFVQNGQK ILAPAPTFDG IPASPNLTPE FCSTQFDVFT DRNRFREVGD FPQLNAALRI



PMVLVMSIWA DHYANMLWLD SVYPPEKEGE PGAARGPCAQ DSGVPSEVKA NYPNAKVVWS NIRFGPIGST VNV


 


SEQ ID NO: 89
MYQRALLFSF FLAAARAQQA GTVTAENHPS LTWQQCSSGG SCTTQNGKVV IDANWRWVHT TSGYTNCYTG NTWDTSICPD DVTCAQNCAL



DGADYSGTYG VTTSGNALRL NFVTQSSGKN IGSRLYLLQD DTTYQIFKLL GQEFTFDVDV SNLPCGLNGA LYFVAMDADG GLSKYPGNKA



GAKYGTGYCD SQCPRDLKFI NGQANVEGWQ PSANDPNAGV GNHGSCCAEM DVWEANSIST AVTPHPCDTP GQTMCQGDDC GGTYSSTRYA



GTCDPDGCDF NPYRQGNHSF YGPGKIVDTS SKFTVVTQFI TDDGTPSGTL TEIKRFYVQN GKVIPQSEST ISGVTGNSIT TEYCTAQKAA



FGDNTGFFTH GGLQKISQAL AQGMVLVMSL WDDHAANMLW LDSTYPTDAD PDTPGVARGT CPTTSGVPAD VESQNPNSYV IYSNIKVGPI NSTFTAN


 


SEQ ID NO: 90
MFAIVLLGLT RSLGTGTNQA ENHPSLSWQN CRSGGSCTQT SGSVVLDSNW RWTHDSSLTN CYDGNEWSSS LCPDPKTCSD NCLIDGADYS



GTYGITSSGN SLKLVFVTNG PYSTNIGSRV YLLKDESHYQ IFDLKNKEFT FTVDDSNLDC GLNGALYFVS MDEDGGTSRF SSNKAGAKYG



TGYCDAQCPH DIKFINGEAN VENWKPQTND ENAGNGRYGA CCTEMDIWEA NKYATAYTPH ICTVNGEYRC DGSECGDTDS GNRYGGVCDK



DGCDFNSYRM GNTSFWGPGL IIDTGKPVTV VTQFVTKDGT DNGQLSEIRR KYVQGGKVIE NTVVNIAGMS SGNSITDDFC NEQKSAFGDT NDFEKKGGLS GLGKAFDYGM



VLVLSLWDDH QVNMLWLDSI YPTDQPASQP GVKRGPCATS SGAPSDVESQ HPDSSVTFSD IRFGPIDSTY


 


SEQ ID NO: 91
MHQRALLFSA LVGAVRAQQA GTLTEEVHPP LTWQKCTADG SCTEQSGSVV IDSNWRWLHS TNGSTNCYTG NTWDESLCPD NEACAANCAL



DGADYESTYG ITTSGDALTL TFVTGENVGS RVYLMAEDDE SYQTFDLVGN EFTFDVDVSN LPCGLNGALY FTSMDADGGV SKYPANKAGA



KYGTGYCDSQ CPRDLKFING MANVEGWTPS DNDKNAGVGG HGSCCPELDI WEANSISSAF TPHPCDDLGQ TMCSGDDCGG TYSETRYAGT



CDPDGCDFNA YRMGNTSYYG PDKIVDTNSV MTVVTQFIGD GGSLSEIKRL YVQNGKVIAN AQSNVDGVTG NSITSDFCTA QKTAFGDQDI



FSKHGGLSGM GDAMSAMVLI LSIWDDHNSS MMWLDSTYPE DADASEPGVA RGTCEHGVGD PETVESQHPG ATVTFSKIKF GPIGSTYSSN STA


 


SEQ ID NO: 92
MFRAAALLAF TCLAMVSGQQ AGTNTAENHP QLQSQQCTTS GGCKPLSTKV VLDSNWRWVH STSGYTNCYT GNEWDTSLCP DGKTCAANCA



LDGADYSGTY GITSTGTALT LKFVTGSNVG SRVYLMADDT HYQLLKLLNQ EFTFDVDMSN LPCGLNGALY LSAMDADGGM SKYPGNKAGA



KYGTGYCDSQ CPKDIKFING EANVGNWTET GSNTGTGSYG TCCSEMDIWE ANNDAAAFTP HPCTTTGQTR CSGDDCARNT GLCDGDGCDF



NSFRMGDKTF LGKGMTVDTS KPFTVVTQFL TNDNTSTGTL SEIRRIYIQN GKVIQNSVAN IPGVDPVNSI TDNFCAQQKT AFGDTNWFAQ



KGGLKQMGEA LGNGMVLALS IWDDHAANML WLDSDYPTDK DPSAPGVARG TCATTSGVPS DVESQVPNSQ VVFSNIKFGD IGSTFSGTSS



PNPPGGSTTS SPVTTSPTPP PTGPTVPQWG QCGGIGYSGS TTCASPYTCH VLNPCESILS LQRSSNADQY LQTTRSATKR RLDTALQPRK


 


SEQ ID NO: 93
MRTALALILA LAAFSAVSAQ QAGTITAETH PTLTIQQCTQ SGGCAPLTTK VVLDVNWRWI HSTTGYTNCY SGNTWDAILC PDPVTCAANC



ALDGADYTGT FGILPSGTSV TLRPVDGLGL RLFLLADDSH YQMFQLLNKE FTFDVEMPNM RCGSSGAIHL TAMDADGGLA KYPGNQAGAK



YGTGFCSAQC PKGVKFINGQ ANVEGWLGTT ATTGTGFFGS CCTDIALWEA NDNSASFAPH PCTTNSQTRC SGSDCTADSG LCDADGCNFN



SFRMGNTTFF GAGMSVDTTK LFTVVTQFIT SDNTSMGALV EIHRLYIQNG QVIQNSVVNI PGINPATSIT DDLCAQENAA FGGTSSFAQH



GGLAQVGEAL RSGMVLALSI VNSAADTLWL DSNYPADADP SAPGVARGTC PQDSASIPEA PTPSVVFSNI KLGDIGTTFG AGSALFSGRS PPGPVPGSAP ASSATATAPP



FGSQCGGLGY AGPTGVCPSP YTCQALNIYY SQCI


 


SEQ ID NO: 94
MYQRALLFSF FLAAARAHEA GTVTAENHPS LTWQQCSSGG SCTTQNGKVV IDANWRWVHT TSGYTNCYTG NTWDTSICPD DVTCAQNCAL



DGADYSGTYG VTTSGNALRL NFVTQSSGKN IGSRLYLLQD DTTYQIFKLL GQEFTFDVDV SNLPCGLNGA LYFVAMDADG NLSKYPGNKA



GAKYGTGYCD SQCPRDLKFI NGQANVEGWQ PSANDPNAGV GNHGSSCAEM DVWEANSIST AVTPHPCDTP GQTMCQGDDC GGTYSSTRYA



GTCDTDGCDF NPYQPGNHSF YGPGKIVDTS SKFTVVTQFI TDDGTPSGTL TEIKRFYVQN GKVIPQSEST ISGVTGNSIT TEYCTAQKAA



FDNTGFFTHG GLQKISQALA QGMVLVMSLW DDHAANMLWL DSTYPTDADP DTPGVARGTC PTTSGVPADV ESQNPNSYVI YSNIKVGPIN STFTAN


 


SEQ ID NO: 95
MHKRAATLSA LVVAAAGFAR GQGVGTQQTE THPKLTFQKC SAAGSCTTQN GEVVIDANWR WVHDKNGYTN CYTGNEWNTT ICADAASCAS



NCVVDGADYQ GTYGASTSGN ALTLKFVTKG SYATNIGSRM YLMASPTKYA MFTLLGHEFA FDVDLSKLPC GLNGAVYFVS MDEDGGTSKY



PSNKAGAKYG TGYCDSQCPR DLKFIDGKAN SASWQPSSND QNAGVGGMGS CCAEMDIWEA NSVSAAYTPH PCQNYQQHSC SGDDCGGTYS



ATRFAGDCDP DGCDWNAYRM GVHDFYGNGK TVDTGKKFSI VTQFKGSGST LTEIKQFYVQ DGRKIENPNA TWPGLEPFNS ITPDFCKAQK



QVFGDPDRFN DMGGFTNMAK ALANPMVLVL SLWDDHYSNM LWLDSTYPTD ADPSAPGKGR GTCDTSSGVP SDVESKNGDA TVIYSNIKFG PLDSTYTAS


 


SEQ ID NO: 96
MRASLLAFSL NSAAGQQAGT LQTKNHPSLT SQKCRQGGCP QVNTTIVLDA NWRWTHSTSG STNCYTGNTW QATLCPDGKT CAANCALDGA



DYTGTYGVTT SGNSLTLQFV TQSNVGARLG YLMADDTTYQ MFNLLNQEFW FDVDMSNLPC GLNGALYFSA MARTAAWMPM VVCASTPLIS



TRRSTARLLR LPVPPRSRYG RGICDSQCPR DIKFINGEAN VQGWQPSPND TNAGTGNYGA CCNKMDVWEA NSISTAYTPH PCTQRGLVRC



SGTACGGGSN RYGSICDHDG LGFQNLFGMG RTRVRARVGR VKQFNRSSRV VEPISWTKQT TLHLGNLPWK SADCNVQNGR VIQNSKVNIP



GMPSTMDSVT TEFCNAQKTA FNDTFSFQQK GGMANMSEAL RRGMVLVLSI WDDHAANMLW LDSITSAAAC RSTPSEVHAT PLRESQIRSS HSRQTRYVTF TNIKFGPFNS



TGTTYTTGSV PTTSTSTGTT GSSTPPQPTG VTVPQGQCGG IGYTGPTTCA SPTTCHVLNP YYSQCY


 


SEQ ID NO: 97
MKQYLQYLAA ALPLMSLVSA QGVGTSTSET HPKITWKKCS SGGSCSTVNA EVVIDANWRW LHNADSKNCY DGNEWTDACT SSDDCTSKCV



LEGAEYGKTY GASTSGDSLS LKFLTKHEYG TNIGSRFYLM NGASKYQMFT LMNNEFAFDV DLSTVECGLN SALYFVAMEE DGGMASYSTN



KAGAKYGTGY CDAQCARDLK FVGGKANYDG WTPSSNDANA GVGALGGCCA EIDVWESNAH AFAFTPHACE NNNYHVCEDT TCGGTYSEDR



FAGDCDANGC DYNPYRVGNT DFYGKGMTVD TSKKFTVVSQ FQENKLTQFF VQNGKKIEIP GPKHEGLPTE SSDITPELCS AMPEVFGDRD



RFAEVGGFDA LNKALAVPMV LVMSIWDDHY ANMLWLDSSY PPEKAGTPGG DRGPCAQDSG VPSEVESQYP DATVVWSNIR FGPIGSTVQV


 


SEQ ID NO: 98
MFPKASLIAL SFIAAVYGQQ VGTQMAEVHP KLPSQLCTKS GCTNQNTAVV LDANWRWLHT TSGYTNCYTG NSWDATLCPD ATTCAQNCAV



DGADYSGTYG ITTSGNALTL KFKTGTNVGS RVYLMQTDTA YQMFQLLNQE FTFDVDMSNL PCGLNGALYL SQMDQDGGLS KFPTNKAGAK



YGTGYCDSQC PHDIKFINGM ANVAGWAGSA SDPNAGSGTL GTCCSEMDIW EANNDAAAFT PHPCSVDGQT QCSGTQCGDD DERYSGLCDK



DGCDFNSFRM GDKSFLGKGM TVDTSRKFTV VTQFVTTDGT TNGDLHEIRR LYVQDGKVIQ NSVVSIPGID AVDSITDNFC AQQKSVFGDT



NYFATLGGLK KMGAALKSGM VLAMSVWDDH AASMQWLDSN YPADGDATKP GVARGTCSAD SGLPTNVESQ SASASVTFSN IKWGDINTTF



TGTGSTSPSS PAGPVSSSTS VASQPTQPAQ GTVAQWGQCG GTGFTGPTVC ASPFTCHVVN PYYSQCY


 


SEQ ID NO: 99
MFRTAALLSF AYLAVVYGQQ AGTSTAETHP PLTWEQCTSG GSCTTQSSSV VLDSNWRWTH VVGGYTNCYT GNEWNTTVCP DGTTCAANCA



LDGADYEGTY GISTSGNALT LKFVTASAQT NVGSRVYLMA PGSETEYQMF NPLNQEFTFD VDVSALPCGL NGALYFSEMD ADGGLSEYPT



NKAGAKYGTG YCDSQCPRDI KFIEGKANVE GWTPSSTSPN AGTGGTGICC NEMDIWEANS ISEALTPHPC TAQGGTACTG DSCSSPNSTA



GICDQAGCDF NSFRMGDTSF YGPGLTVDTT SKITVVTQFI TSDNTTTGDL TAIRRIYVQN GQVIQNSMSN IAGVTPTNEI TTDFCDQQKT



AFGDTNTFSE KGGLTGMGAA FSRGMVLVLS IWDDDAAEML WLDSTYPVGK TGPGAARGTC ATTSGQPDQV ETQSPNAQVV FSNIKFGAIG



STFSSTGTGT GTGTGTGTGT GTTTSSAPAA TQTKYGQCGG QGWTGATVCA SGSTCTSSGP YYSQCL


 


SEQ ID NO: 100
MFRTAALTAF TFAAVVLGQQ VGTLTTENHP ALSIQQCTAT GCTTQQKSVV LDSNWRWTHS TAGATNCYTG NAWDPALCPD PATCATNCAI



DGADYSGTYG ITTSGNALTL RFVTNGQYSQ NIGSRVYLLD DADHYKLFDL KNQEFTFDVD MSGLPCGLNG ALYFSEMAAD GGKAAHAGNN



AGAKYGTGYC DAQCPHDIKW INGEANVLDW SASATDDNAG NGRYGACCAE MDIWEANSEA TAYTPHVCRD EGLYRCSGTE CGDGNNRYGG



VCDKDGCDFN SYRMGDKNFL GRGKTIDTTK KVTVVTQFIT DNNTPTGNLV EIRRVYVQNG VVYQNSFSTF PSLSQYNSIS DEFCVAQKTL



FGDNQYYNTH GGTTKMGDAF DNGMVLIMSL WSDHAAHMLW LDSDYPLDKS PSEPGVSRGA CPTSSGDPDD VVANHPNASV TFSNIKYGPI GSTFGGSTPP VSSGGSSVPP



VTSTTSSGTT TPTGPTGTVP KWGQCGGIGY SGPTACVAGS TCTYSNDWYS QCL


 


SEQ ID NO: 101
MYRAIATASA LIAAVRAQQV CSLTPETKPA LSWSKCTSSG CSNVQGSVTI DANWRWTHQL SGSTNCYTGN KWDTSICTSG KVCAEKCCID



GAEYASTYGI TSSGNQLSLS FVTKGTYGTN IGSRTYLMED ENTYQMFQLL GNEFTFDVDV SNIGCGLNGA LYFVSMDADG GKAKYPGNKA



GAKYGTGYCD AQCPRDVKFI NGQANSDGWQ PSKSDVNGGI GNLGTCCPEM DIWEANSIST AHTPHPCTKL TQHSCTGDSC GGTYSEDRYG



GTCDADGCDF NAYRQGNKTF YGPGSGFNVD TTKKVTVVTQ FHKGSNGRLS EITRLYVQNG KVIANSESKI AGVPGSSLTP EFCTAQKKVF



GDIDDFEKKG AWGGMSDALE APMVLVMSLW HDHHSNMLWL DSTYPTDSTK LGAQRGSCST SSGVPADLEK NVPNSKVAFS NIKFGPIGST



YKEGQPEPTN PTNPNPTTPG GTVDQWGQCG GTNYSGPTAC KSPFTCKKIN DFYSQCQ


 


SEQ ID NO: 102
MFRTATLLAF TMAAMVFGQQ VGTNTAENHR TLTSQKCTKS GGCSNLNTKI VLDANWRWLH STSGYTNCYT GNQWDATLCP DGKTCAANCA



LDGADYTGTY GITASGSSLK LQFVTGSNVG SRVYLMADDT HYQMFQLLNQ EFTFDVDMSN LPCGLNGALY LSAMDADGGM AKYPTNKAGA



KYGTGYCDSQ CPRDIKFING EANVEGWNAT SANAGTGNYG TCCTEMDIWE ANNDAAAYTP HPCTTNAQTR CSGSDCTRDT GLCDADGCDF



NSFRMGDQTF LGKGLTVDTS KPFTVVTQFI TNDGTSAGTL TEIRRLYVQN GKVIQNSSVK IPGIDLVNSI TDNFCSQQKT AFGDTNYFAQ



HGGLKQVGEA LRTGMVLALS IWDDYAANML WLDSNYPTNK DPSTPGVARG TCATTSGVPA QIEAQSPNAY VVFSNIKFGD LNTTYTGTVS SSSVSSSHSS TSTSSSHSSS



STPPTQPTGV TVPQWGQCGG IGYTGSTTCA SPYTCHVLNP YYSQCY


 


SEQ ID NO: 103
MYQTSLLASL SFLLATSQAQ QVGTQTAETH PKLTTQKCTT AGGCTDQSTS IVLDANWRWL HTVDGYTNCY TGQEWDTSIC TDGKTCAEKC



ALDGADYEST YGISTSGNAL TMNFVTKSSQ TNIGGRVYLL AADSDDTYEL FKLKNQEFTF DVDVSNLPCG LNGALYFSEM DSDGGLSKYT



TNKAGAKYGT GYCDTQCPHD IKFINGEANV QNWTASSTDK NAGTGHYGSC CNEMDIWEAN SQATAFTPHV CEAKVEGQYR CEGTECGDGD



NRYGGVCDKD GCDFNSYRMG NETFYGSNGS TIDTTKKFTV VTQFITADNT ATGALTEIRR KYVQNDVVIE NSYADYETLS KFNSITDDFC



AAQKTLSGDT NDFKTKGGIA RMGESFERGM VLVMSVWDDH AANALWLDSS YPTDADASKP GVKRGPCSTS SGVPSDVEAN DADSSVIYSN



IRYGDIGSTF NKTA


 


SEQ ID NO: 104
MFSKVALTAL CFLAVAQAQQ VGREVAENHP RLPWQRCTRN GGCQTVSNGQ VVLDANWRWL HVTDGYTNCY TGNAWNSSVC SDGATCAQRC



ALEGANYQQT YGITTSGDAL TIKFLTRSEQ TNIGARVYLM ENEDRYQMFN LLNKEFTFDV DVSKVPCGIN GALYFIQMDA DGGLSSQPNN



RAGAKYGTGY CDSQCPRDIK FINGEANSVG WEPSETDPNA GKGQYGICCA EMDIWEANSI SNAYTPHPCQ TVNDGGYQRC QGRDCNQPRY



EGLCDPDGCD YNPFRMGNKD FYGPGKTVDT NRKMTVVTQF ITHDNTDTGT LVDIRRLYVQ DGRVIANPPT NFPGLMPAHD SITQEFCDDA



KRAFEDNDSF GRNGGLAHMG RSLAKGHVLA LSIWNDHTAH MLWLDSNYPT DADPNKPGIA RGTCPTTGGS PRDTEQNHPD AQVIFSNIKF



GDIGSTFSGN


 


SEQ ID NO: 105
MYRKLAVISA FLAAARAQQV CTQQAETHPP LTWQKCTASG CTPQQGSVVL DANWRWTHDT KSTTNCYDGN TWSSTLCPDD ATCAKNCCLD



GANYSGTYGV TTSGDALTLQ FVTASNVGSR LYLMANDSTY QEFTLSGNEF SFDVDVSQLP CGLNGALYFV SMDADGGQSK YPGNAAGAKY



GTGYCDSQCP RDLKFINGQA NVEGWEPSSN NANTGVGGHG SCCSEMDIWE ANSISEALTP HPCETVGQTM CSGDSCGGTY SNDRYGGTCD



PDGCDWNPYR LGNTSFYGPG SSFALDTTKK LTVVTQFATD GSISRYYVQN GVKFQQPNAQ VGSYSGNTIN TDYCAAEQTA FGGTSFTDKG



GLAQINKAFQ GGMVLVMSLW DDYAVNMLWL DSTYPTNATA STPGAKRGSC STSSGVPAQV EAQSPNSKVI YSNIRFGPIG STGGNTGSNP



PGTSTTRAPP SSTGSSPTAT QTHYGQCGGT GWTGPTRCAS GYTCQVLNPF YSQCL


 


SEQ ID NO: 106
MRASLLAFSL AAAVAGGQQA GTLTAKRHPS LTWQKCTRGG CPTLNTTMVL DANWRWTHAT SGSTKCYTGN KWQATLCPDG KSCAANCALD



GADYTGTYGI TGSGWSLTLQ FVTDNVGARA YLMADDTQYQ MLELLNQELW FDVDMSNIPC GLNGALYLSA MDADGGMRKY PTNKAGAKYA



TGYCDAQCPR DLKYINGIAN VEGWTPSTND ANGIGDHGSC CSEMDIWEAN KVSTAFTPHP CTTIEQHMCE GDSCGGTYSD DRYGVLCDAD



GCDFNSYRMG NTTFYGEGKT VDTSSKFTVV TQFIKDSAGD LAEIKAFYVQ NGKVIENSQS NVDGVSGNSI TQSFCKSQKT AFGDIDDFNK



KGGLKQMGKA LAQAMVLVMS IWDDHAANML WLDSTYPVPK VPGAYRGSGP TTSGVPAEVD ANAPNSKVAF SNIKFGHLGI SPFSGGSSGT



PPSNPSSSAS PTSSTAKPSS TSTASNPSGT GAAHWAQCGG IGFSGPTTCP EPYTCAKDHD IYSQCV


 


SEQ ID NO: 107
MLASTFSYRM YKTALILAAL LGSGQAQQVG TSQAEVHPSM TWQSCTAGGS CTTNNGKVVI DANWRWVHKV GDYTNCYTGN TWDKTLCPDD



ATCASNCALE GANYQSTYGA TTSGDSLRLN FVTTSQQKNI GSRLYMMKDD TTYEMFKLLN QEFTFDVDVS NLPCGLNGAL YFVAMDADGG



MSKYPTNKAG AKYGTGYCDS QCPRDLKFIN GQANVEGWQP SSNDANAGTG NHGSCCAEMD IWEANSISTA FTPHPCDTPG QVMCTGDACG



GTYSSDRYGG TCDPDGCDFN SFRQGNKTFY GPGMTVDTKS KFTVVTQFIT DDGTASGTLK EIKRFYVQNG KVIPNSESTW SGVGGNSITN



DYCTAQKSLF KDQNVFAKHG GMEGMGAALA QGMVLVMSLW DDHAANMLWL DSNYPTTASS STPGVARGTC DISSGVPADV EANHPDASVV



YSNIKVGPIG STFNSGGSNP GGGTTTTAKP TTTTTTAGSP GGTGVAQHYG QCGGNGWQGP TTCASPYTCQ KLNDFYSQCL


 


SEQ ID NO: 108
MQIKQYLQYL AAALPLVNMA AAQRAGTQQT ETHPRLSWKR CSSGGNCQTV NAEIVIDANW RWLHDSNYQN CYDGNRWTSA CSSATDCAQK



CYLEGANYGS TYGVSTSGDA LTLKFVTKHE YGTNIGSRVY LMNGSDKYQM FTLMNNEFAF DVDLSKVECG LNSALYFVAM EEDGGMRSYS



SNKAGAKYGT GYCDAQCARD LKFVGGKANI EGWRPSTNDA NAGVGPYGAC CAEIDVWESN AYAFAFTPHG CLNNNYHVCE TSNCGGTYSE



DRFGGLCDAN GCDYNPYRMG NKDFYGKGKT VDTSRKFTVV TRFEENKLTQ FFIQDGRKID IPPPTWPGLP NSSAITPELC TNLSKVFDDR



DRYEETGGFR TINEALRIPM VLVMSIWDGH YASMLWLDSV YPPEKAGQPG AERGPCAPTS GVPAEVEAQF PNAQVIWSNI RFGPIGSTYQ V


 


SEQ ID NO: 109
MTSRIALVSL FAAVYGQQVG TYQTETHPSL TWQSCTAKGS CTTNTGSIVL DGNWRWTHGV GTSTNCYTGN TWDATLCPDD ATCAQNCALE



GADYSGTYGI TTSGNSLRLN FVTQSANKNI GSRVYLMADT THYKTFNLLN QEFTFDVDVS NLPCGLNGAV YFANLPADGG ISSTNTAGAE



YGTGYCDSQC PRDMKFIKGQ ANVDGWVPSS NNANTGVGNH GSCCAEMDIW EANSISTAVT PHSCDTVTQT VCTGDDCGGT YSSSRYAGTC



DPDGCDFNSY RMGDETFYGP GKTVDTNSVF TVVTQFLTTD GTASGTLNEI KRFYVQDGKV IPNSYSTISG VSGNSITTPF CDAQKTAFGD



PTSFSDHGGL ASMSAAFEAG MVLVLSLWDD YYANMLWLDS TYPVGKTSAG GPRGTCDTSS GVPASVEASS PNAYVVYSNI KVGAINSTYG


 


SEQ ID NO: 110
MFVFVLLWLT QSLGTGTNQA ENHPSLSWQN CRSGGSCTQT SGSVVLDSNW RWTHDSSLTN CYDGNEWSSS LCPDPKTCSD NCLIDGADYS



GTYGITSSGN SLKLVFVTNG PYSTNIGSRV YLLKDESHYQ IFDLKNKEFT FTVDDSNLDC GLNGALYFVS MDEDGGTSRF SSNKAGAKYG



TGYCDAQCPH DIKFINGEAN VENWKPQTND ENAGNGRYGA CCTEMDIWEA NKYATAYTPH ICTVNGEYRC DGSECGDTDS GNRYGGVCDK



DGCDFNSYRM GNTSFWGPGL IIDTGKPVTV VTQFVTKDGT DNGQLSEIRR KYVQGGKVIE NTVVNIAGMS SGNSITDDFC NEQKSAFGDT



NDFEKKGGLS GLGKAFDYGM VLVLSLWDDH QVNMLWLDSI YPTDQPASQP GVKRGPCATS SGAPSDVESQ HPDSSVTFSD IRFGPIDSTY


 


SEQ ID NO: 111
MFRKAALLAF SFLAIAHGQQ VGTNQAENHP SLPSQKCTAS GCTTSSTSVV LDANWRWVHT TTGYTNCYTG QTWDASICPD GVTCAKACAL



DGADYSGTYG ITTSGNALTL QFVKGTNVGS RVYLLQDASN YQMFQLINQE FTFDVDMSNL PCGLNGAVYL SQMDQDGGVS RFPTNTAGAK



YGTGYCDSQC PRDIKFINGE ANVEGWTGSS TDSNSGTGNY GTCCSEMDIW EANSVAAAYT PHPCSVNQQT RCTGADCGQG DDRYDGVCDP



DGCDFNSFRM GDQTFLGKGL TVDTSRKFTI VTQFISDDGT TSGNLAEIRR FYVQDGNVIP NSKVSIAGID AVNSITDDFC TQQKTAFGDT



NRFAAQGGLK QMGAALKSGM VLALSLWDDH AANMLWLDSD YPTTADASNP GVARGTCPTT SGFPRDVESQ SGSATVTYSN IKWGDLNSTF



TGTLTTPSGS SSPSSPASTS GSSTSASSSA SVPTQSGTVA QWAQCGGIGY SGATTCVSPY TCHVVNAYYS QCY


 


SEQ ID NO: 112
MYRAIATASA LIAAARAQQV CTLTTETKPA LTWSKCTSSG CTDVKGSVGI DANWRWTHQT SSSTNCYTGN KWDTSVCTSG ETCAQKCCLD



GADYAGTYGI TSSGNQLSLG FVTKGSFSTN IGSRTYLMEN ENTYQMFQLL GNEFTFDVDV SNIGCGLNGA LYFVSMDADG GKARYPANKA



GAKYGTGYCD AQCPRDVKFI NGKANSDGWK PSDSDINAGI GNMGTCCPEM DIWEANSIST AFTPHPCTKL TQHACTGDSC GGTYSNDRYG



GTCDADGCDF NSYRQGNKTF YGRGSDFNVD TTKKVTVVTQ FKKGSNGRLS EITRLYVQNG KVIANSESKI PGNSGSSLTA DFCSKQKSVF



GDIDDFSKKG GWSGMSDALE SPPMVLVMSL WHDHHSNMLW LDSTYPTDST KLGAQRGSCA TTSGVPSDLE RDVPNSKVSF SNIKFGPIGS



TYSSGTTNPP PSSTDTSTTP TNPPTGGTVG QYGQCGGQTY TGPKDCKSPY TCKKINDFYS QCQ


 


SEQ ID NO: 113
MSSFQIYRAA LLLSILATAN AQQVGTYTTE THPSLTWQTC TSDGSCTTND GEVVIDANWR WVHSTSSATN CYTGNEWDTS ICTDDVTCAA



NCALDGATYE ATYGVTTSGS ELRLNFVTQG SSKNIGSRLY LMSDDSNYEL FKLLGQEFTF DVDVSNLPCG LNGALYFVAM DADGGTSEYS



GNKAGAKYGT GYCDSQCPRD LKFINGEANC DGWEPSSNNV NTGVGDHGSC CAEMDVWEAN SISNAFTAHP CDSVSQTMCD GDSCGGTYSA



SGDRYSGTCD PDGCDYNPYR LGNTDFYGPG LTVDTNSPFT VVTQFITDDG TSSGTLTEIK RLYVQNGEVI ANGASTYSSV NGSSITSAFC



ESEKTLFGDE NVFDKHGGLE GMGEAMAKGM VLVLSLWDDY AADMLWLDSD YPVNSSASTP GVARGTCSTD SGVPATVEAE SPNAYVTYSN



IKFGPIGSTY SSGSSSGSGS SSSSSSTTTK ATSTTLKTTS TTSSGSSSTS AAQAYGQCGG QGWTGPTTCV SGYTCTYENA YYSQCL


 


SEQ ID NO: 114
MHQRALLFSA LLTAVRAQQA GTLTEEVHPS LTWQKCTSEG SCTEQSGSVV IDSNWRWTHS VNDSTNCYTG NTWDATLCPD DETCAANCAL



DGADYESTYG VTTDGDSLTL KFVTGSNVGS RLYLMDTSDE GYQTFNLLDA EFTFDVDVSN LPCGLNGALY FTAMDADGGV SKYPANKAGA



KYGTGYCDSQ CPRDLKFIDG QANVDGWEPS SNNDNTGIGN HGSCCPEMDI WEANKISTAL TPHPCDSSEQ TMCEGNDCGG TYSDDRYGGT



CDPDGCDFNP YRMGNDSFYG PGKTIDTGSK MTVVTQFITD GSGSLSEIKR YYVQNGNVIA NADSNISGVT GNSITTDFCT AQKKAFGDED



IFAEHNGLAG ISDAMSSMVL ILSLWDDYYA SMEWLDSDYP ENATATDPGV ARGTCDSESG VPATVEGAHP DSSVTFSNIK FGPINSTFSA SA


 


SEQ ID NO: 115
MYAKFATLAA LVAGAAAQNA CTLTAENHPS LTWSKCTSGG SCTSVQGSIT IDANWRWTHR TDSATNCYEG NKWDTSYCSD GPSCASKCCI



DGADYSSTYG ITTSGNSLNL KFVTKGQYST NIGSRTYLME SDTKYQMFQL LGNEFTFDVD VSNLGCGLNG ALYFVSMDAD GGMSKYSGNK



AGAKYGTGYC DSQCPRDLKF INGEANVENW QSSTNDANAG TGKYGSCCSE MDVWEANNMA AAFTPHPCXV IGQSRCEGDS CGGTYSTDRY



AGICDPDGCD FNSYRQGNKT FYGKGMTVDT TKKITVVTQF LKNSAGELSE IKRFYVQNGK VIPNSESTIP GVEGNSITQD WCDRQKAAFG



DVTDXQDKGG MVQMGKALAG PMVLVMSIWD DHAVNMLWLD STWPIDGAGK PGAERGACPT TSGVPAEVEA EAPNSNVIFS NIRFGPIGST VSGLPDGGSG NPNPPVSSST



PVPSSSTTSS GSSGPTGGTG VAKHYEQCGG IGFTGPTQCE SPYTCTKLND WYSQCL


 


SEQ ID NO: 116
MYAKFATLAA LVAGASAQAV CSLTAETHPS LTWQKCTAPG SCTNVAGSIT IDANWRWTHQ TSSATNCYSG SKWDSSICTT GTDCASKCCI



DGAEYSSTYG ITTSGNALNL KFVTKGQYST NIGSRTYLME SDTKYQMFKL LGNEFTFDVD VSNLGCGLNG ALYFVSMDAD GGMSKYSGNK



AGAKYGTGYC DAQCPRDLKF INGEANVEGW ESSTNDANAG SGKYGSCCTE MDVWEANNMA TAFTPHPCTT IGQTRCEGDT CGGTYSSDRY



AGVCDPDGCD FNSYRQGNKT FYGKGMTVDT TKKITVVTQF LKNSAGELSE IKRFYAQDGK VIPNSESTIA GIPGNSITKA YCDAQKTVFQ



NTDDFTAKGG LVQMGKALAG DMVLVMSVWD DHAVNMLWLD STYPTDQVGV AGAERGACPT TSGVPSDVEA NAPNSNVIFS NIRFGPIGST



VQGLPSSGGT SSSSSAAPQS TSTKASTTTS AVRTTSTATT KTTSSAPAQG TNTAKHWQQC GGNGWTGPTV CESPYKCTKQ NDWYSQCL


 


SEQ ID NO: 117
MLTLVYFLLS LVVSLEIGTQ QSEDHPKLTW QNGSSSVSGS IVLDSNWRWV HDSGTTNCYD GNLWSKDLCP SSDTCSQKCY IEGADYSGTY



GIQSSGSKLT LKFVTKGSYS TNIGSRVYLL KDENTYESFK LKNKEFTFTV DDSKLNCGLN GALYFVAMDA DGGKAKYSSF KPGAKYGMGY



CDAQCPHDMK FISGKANVDD WKPQDNDENS GNGKLGTCCS EMDIWEGNMK SQAYTVHACT KSGQYECTGQ QCGDTDSGDR FKGTCDKDGC



DYASWRWGDQ SFYGEGKTVD TKQPVTVVTQ FIGDPLTEIR RLYVQGGKTI NNSKTSNLAD TYDSITDKFC DATKEASGDT NDFKAKGAMS



GFSTNLNNGQ VLVMSLWDDH TANMLWLDST YPTDSSDSTA QRGPCPTSSG VPKDVESQHG DATVVFSDIK FGAINSTFKY N


 


SEQ ID NO: 118
MLAAALFTFA CSVGVGTKTP ENHPKLNWQN CASKGSCSQV SGEVTMDSNW RWTHDGNGKN CYDGNTWISS LCPDDKTCSD KCVLDGAEYQ



ATYGIQSNGT ALTLKFVTHG SYSTNIGSRL YLLKDKSTYY VFKLNNKEFT FSVDVSKLPC GLNGALYFVE MDADGGKAKY AGAKPGAEYG



LGYCDAQCPS DLKFINGEAN SEGWKPQSGD KNAGNGKYGS CCSEMDVWES NSQATALTPH VCKTTGQQRC SGKSECGGQD GQDRFAGLCD



EDGCDFNNWR MGDKTFFGPG LIVDTKSPFV VVTQFYGSPV TEIRRKYVQN GKVIENSKSN IPGIDATAAI SDHFCEQQKK AFGDTNDFKN



KGGFAKLGQV FDRGMVLVLS LWDDHQVAML WLDSTYPTNK DKSQPGVDRG PCPTSSGKPD DVESASADAT VVYGNIKFGA LDSTY


 


SEQ ID NO: 119
MLTLVYFLLS LVVSLEIGTQ QSEDHPKLTW QNGSSSVSGS IVLDSNWRWV HDSGTTNCYD GNLWSKDLCP SSNTCSQKCY IEGADYSGTY



GIQSSGSKLT LKFVTKGSYS TNIGSRVYLL KDENTYESFK LKNKEFTFTV DDSKLNCGLN GALYFVAMDA DGGKAKYSSF KPGAKYGMGY



CDAQCPHDMK FISGKANVDD WKPQDNDENS GNGKLGTCCS EMDIWEGNMK SQAYTVHACT KSGQYECTGQ QCGDTDSGDR FKGTCDKDGC



DYASWRWGDQ SFYGEGKTVD TKQPVTVVTQ FIGDPLTEIR RLYVQGGKTI NNSKTSNLAD TYDSITDKFC DATKEASGDT NDFKAKGAMS GFSTNLNNGQ VLVMSLWDDH



TANMLWLDST YPTDSTKTGA SRGPCAVSSG VPKDVESQYG DATVIYSDIK FGAINSTFKW N


 


SEQ ID NO: 120
MILALLSLAK SLGIATNQAE THPKLTWTRY QSKGSGQTVN GEIVLDSNWR WTHHSGTNCY DGNTWSTSLC PDPTTCSNNC DLDGADYPGT



YGISTSGNSL KLGFVTHGSY STNIGSRVYL LRDSKNYEMF KLKNKEFTFT VDDSKLPCGL NGALYFVAMD EDGGVSKNSI NKAGAQYGTG



YCDAQCPHDM KFINGEANVL DWKPQSNDEN SGNGRYGACC TEMDIWEANS MATAYTPHVC TVTGLRRCEG TECGDTDANQ RYNGICDKDG



CDFNSYRLGD KTFFGVGKTV DSSKPVTVVT QFVTSNGQDS GTLSEIRRKY VQGGKVIENS KVNIAGITAG NSVTDTFCNE QKKAFGDNND



FEKKGGLGAL SKQLDAGMVL VLSLWDDHSV NMLWLDSTYP TNAAAGALGT ERGACATSSG APSDVESQSP DATVTFSDIK FGPIDSTY


 


SEQ ID NO: 121
MLVIALILRG LSVGTGTQQS ETHPSLSWQQ TSKGGSGQSV SGSVVLDSNW RWTHTTDGTT NCYDGNEWSS DLCPDASTCS SNCVLEGADY



SGTYGITGSG SSLKLGFVTK GSYSTNIGSR VYLLGDESHY KLFKLENNEF TFTVDDSNLE CGLNGALYFV AMDEDGGASK YSGAKPGAKY



GMGYCDAQCP HDMKFINGDA NVEGWKPSDN DENAGTGKWG ACCTEMDIWE ANKYATAYTP HICTKNGEYR CEGTDCGDTK DNNRYGGVCD



KDGCDFNSWR MGNQSFWGPG LIIDTGKPVT VVTQFLADGG SLSEIRRKYV QGGKVIENTV TKISGMDEFD SITDEFCNQQ KKAFRDTNDF



EKKGGLKGLG TAVDAGVVLV LSLWDDHDVN MLWLDSIYPT DSGSKAGADR GPCATSSGVP KDVESNYASA SVTFSDIKFG PIDSTY


 


SEQ ID NO: 122
MLLALFAFGK SLGIATNQAE NHPKLTWTRY QSKGSGQTVN GEIVLDSNWR WTHHSGTNCY DGNTWSTSLC PDPTTCSNNC DLDGADYPGT



YGISSSGNSL KLGFVTHGSY STNIGSRVYL LRDSKNYEMF KLKNKEFTFT VDDSKLPCGL NGALYFVAMD EDGGVSKNSI NKAGAQYGTG



YCDAQCPHDM KFINGEANVL DWKPQSNDEN SGNGRYGACC TEMDIWEANS MATAYTPHVC TVTGIRRCEG TECGDTDANQ RYNGICDKDG



CDFNSYRLGD KSFFGVGKTV DSSKPVTVVT QFVTSNGQDS GTLSEIRRKY VQGGKVIENS KVNIAGMAAG NSITDTFCNE QKKAFGDNND FEKKGGLGAL SKQLDSGMVL



VLSLWDDHSV NMLWLDSTYP TNAAAGALGT ERGACATSSG APSDVESQSP DATVTFSDIK FGPIDSTY


 


SEQ ID NO: 123
MLASVVYLVS LVVSLEIGTQ QSEEHPKLTW QNGSSSVSGS IVLDSNWRWL HDSGTTNCYD GNLWSDDLCP NADTCSSKCY IEGADYSGTY



GITSSGSKVT LKFVTKGSYS TNIGSRIYLL KDENTYETFK LKNKEFTFTV DDSKLDCGLN GALYFVAMDA DGGKAKYSSF KPGAKYGMGY



CDAQCPHDMK FISGKANVDD WKPQDNDENS GDGKLGTCCS EMDIWEGNAK SQAYTVHACS KSGQYECTGQ QCGDTDSGDR FKGTCDKDGC



DYASWRWGDQ SFYGEGKTVD TKSPVTVVTQ FIGDPLTEIR RVYVQGGKTI NNSKTSNLAD TYDSITDKFC DATKDATGDT NDFKAKGAMA



GFSTNLNTAQ VLVSVHCGMI IQPICCGLIR RIQRIQQKQV QAVDRVLCRR VFQRMLKASM VMLQSRTRTL SLELSTRPLV GISPAGRLFF F


 


SEQ ID NO: 124
MILALLVLGK SLGIATNQAE THPKLTWTRY QSKGSGSTVN GEIVLDSNWR WTHHSGTNCY DGNTWSTSLC PDPTTCSNNC DLDGADYPGT



YGISTSGNSL KLGFVTHGSY STNIGSRVYL LKDTKSYEMF KLKNKEFTFT VDDSKLPCGL NGALYFVAMD EDGGVSKNSI NKAGAQYGTG



YCDAQCPHDM KFINGEANVL DWKPQSNDEN SGNGRYGACC TEMDIWEANS MATAYTPHVC TVTGLRRCEG TECGDTDNDQ RYNGICDKDG



CDFNSYRLGD KSFFGVGKTV DSSKPVTVVT QFVTSNGQDS GTLSEIRRKY VQGGKVIENS KVNVAGITAG NSVTDTFCNE QKKAFGDNND



FEKKGGLGAL SKQLDAGMVL VLSLWDDHSV NMLWLDSTYP TNAAAGALGT ERGACATSSG KPSDVESQSP DATVTFSDIK FGPIDSTY


 


SEQ ID NO: 125
MLCIGLISFV YSLGVGTNTA ETHPKLTWKN GGQTVNGEVT VDSNWRWTHT KGSTKNCYDG NLWSKDLCPD AATCGKNCVL EGADYSGTYG



VTSSGNALTL KFVTHGSYST NVGSRLYLLK DEKTYQMFNL NGKEFTFTVD VSNLPCGLNG ALYHVNMDED GGTKRYPDNE AGAKYGTGYC



DAQCPTDLKF INGIPNSDGW KPQSNDKNSG NGKYGSCCSE MDIWEANSIC SAVTPHVCDN LQQTRCQGTA CGENGGGSRF GSSCDPDGCD



FNSWRMGNKT FYGPGLIVDT KSKFTVVTQF VGNPVTEIKR KYVQNGKVIE NSYSNIEGMD KFNSVSDKFC TAQKKAFGDT DSFTKHGGFK



QLGSALAKGM VLVLSLWDDH TVNMLWLDSV YPTNSKKAGS DRGPCPTTSG VPADVESKSA DANVIYSDIR FGAIDSTYK


 


SEQ ID NO: 126
MLGALVALAS CIGVGTNTPE KHPDLKWTNG GSSVSGSIVV DSNWRWTHIK GETKNCYDGN LWSDKYCPDA ATCGKNCVLE GADYSGTYGV



TTSGDAATLK FVTHGQYSTN VGSRLYLLKD EKTYQMFNLV GKEFTFTVDV SNLPCGLNGA LYFVQMDSDG GMAKYPDNQA GAKYGTGYCD



AQCPTDLKFI NGIPNSDGWK PQKNDKNSGN GKYGSCCSEM DIWEANSMAT AYTPHVCDKL EQTRCSGSAC GQNGGGDRFS SSCDPDGCDF



NSWRMGNKTF WGPGLIVDTK KPVQVVTQFV GSGGSVTEIK RKYVQGGKVI DNSMTNIAAM SKQYNSVSDE FCQAQKKAFG DNDSFTKHGG FRQLGATLSK GHVLVLSLWD



DHDVNMLWLD SVYPTNSNKP GADRGPCKTS SGVPSDVESQ NADSTVKYSD IRFGAIDSTY SK


 


SEQ ID NO: 127
MLAAALFTFA CSVGVGTKTT ETHPKLNWQQ CACKGSCSQV SGEVTMDSNW RWTHDGNGKN CYDGNTWISS LCPDDKTCSD KCVLDGAEYQ



ATYGIQSNGT ALTPKFVTHG SYSTNIGSRL YLLKDKSTYY VFQLNNKEFT FSVDVSKLPC GLNGALYFVE MDADGGKSKY AGAKPGAEYG



LGYCDAQCPS DLKFINGEAN SEGWKPQSGD KNAGNGKYGS CCSEMDVWES NSMATALTPH VCKTTGQTRC SGKSECGGQD GQDRFAGNCD



EDGCDFNNWR MGDKTFFGPG LTVDTKSPFV VVTQFYGSPV TEIRRKYVQN GKVIENAKSN IPGIDATNAI SDTFCEQQKK AFGDTNDFKN



KGGFTKLGSV FSRGMVLVLS LWDDHQVAML WLDSTYPTNK DKSVPGVDRG PCPTSSGKPD DVESASGDAT VVYGNIKFGA LDSTY


 


SEQ ID NO: 128
MFGFLLSLFA LQFALEIGTQ TSESHPSITW ELNGARQSGQ IVIDSNWRWL HDSGTTNCYD GNTWSSDLCP DPEKCSQNCY LEGADYSGTY



GISASGSQLT LGFVTKGSYS TNIGSRVYLL KDENTYPMFK LKNKEFTFTV DVSNLPCGLN GALYFVAMPS DGGKAKYPLA KPGAKYGMGY



CDAQCPHDMK FINGEANVLD WKPQSNDENA GTGRYGTCCT EMDIWEANSQ ATAYTVHACS KNARCEGTEC GDDSASQRYN GICDKDGCDF



NSWRWGNKTF FGPGLTVDSS KPVTVVTQFI GDPLTEIRRI WVQGGKVIQN SFTNVSGITS VDSITNTFCD ESKVATGDTN DFKAKGGMSG



FSKALDTEVV LVLSLWDDHT ANMLWLDSTY PTDSTAIGAS RGPCATSSGD PKDVESASAN ASVKFSDIKF GALDSTY


 


SEQ ID NO: 129
MLASLLPLSN SLGTASNQAE THPKLTWTQY TGKGAGQTVN GEIVLDSNWR WTHKDGTNCY DGNTWSSSLC PDPTTCSNNC NLDGADYPGT



YGITTSGNQL KLGFVTHGSY STNIGSRVYL LRDSKNYQMF KLKNKEFTFT VDDSKLPCGL NGAVYFVAMD EDGGTAKHSI NKAGAQYGTG



YCDAQCPHDM KFINGEANVL DWKPQSNDEN SGNGRWGARC TEMDIWEANS RATAYTPHIC TKTGLYRCEG TECGDSDTNR YGGVCDKDGC



DFNSYRMGDK SFFGQGKTVD SSKPVTVVTQ FITDNNQDSG KLTEIRRKYV QGGKVIDNSK VNIAGITAGN PITDTFCDEA KKAFGDNNDF



EKKGGLSALG TQLEAGFVLV LSLWDDHSVN MLWLDSTYPT NASPGALGVE RGDCAITSGV PADVESQSAD ASVTFSDIKF GPIDSTY


 


SEQ ID NO: 130
MLCIGLISFV YSLGVGTNTA ETHPKLTWKN GGQTVNGEVT VDSNWRWTHT KGSTKNCYDG NLWSKDLCPD AATCGKNCVL EGADYSGTYG



VTSSGNALTL KFVTHGSYST NVGSRLYLLK DEKTYQMFNL NGKEFTFTVD VSNLPCGLSG ALYHVNMDED GGTKRYPDNE AGAKYGTGYC



DAQCPTDLKF INGIPNSDGW KPQSNDKNSG NGKYGSCCSE MDIWEANSIC SAVTPHVCDN LQQTRCQGAA CGENGGGSRF GSSCDPDGCD



FNSWGMGNKT FYGPGLIVDT KSKFTVVTQF VGNPVTEIKR KYVQNGKVIE NSYSNIEGMD KFNSVSDKFC TAQKKAFGDT DSFTKHGGFK



QLGSALAKGM VLVLSLWDDH TVNMLWLDSV YPTNSKKAGS DRGPCPTTSG VPADVESKSA DANVIYSDIR FGAIDSTYK


 


SEQ ID NO: 131
MILALLVLGK SLGIATNQAE THPKLTWTRY QSKGSGSTVN GEIVLDSNWR WTHHSGTNCY DGNTWSTSLC PDPTTCSNNC DLDGADYPGT



YGISTSGNSL KLGFVTHGSY STNIGSRVYL LRDSKNYEMF KLKNKEFTFT VDDSKLPCGL NGALYFVAMD EDGGVSKNSI NKAGAQYGTG



YCDAQCPHDM KFINGEANVL DWKPQSNDEN SGNGRYGACC TEMDIWEANS MATAYTPHVC TVTGLRRCEG TECGDTDNDQ RYNGICDKDG



CDFNSYRLGD KSFFGVGKTV DSSKPVTVVT QFVTSNGQDS GILSETRRKY VQGGKVIENS KVNVAGITAG NSVTDTFCNE QKKAFGDNND FEKKGGLGAL SKQLDAGMVL



VLSLWDDHSV NMLWLDSTYP TNAAAGALGT ERGACATSSG KPSDVESQSP DATVTFSDIK FGPIDSTY


 


SEQ ID NO: 132
MIGIVLIQTV FGIGVGTQQS ESHPSLSWQQ CSKGGSCTSV SGSIVLDSNW RWTHIPDGTT NCYDGNEWSS DLCPDPTTCS NNCVLEGADY



SGTYGISTSG SSAKLGFVTK GSYSTNIGSR VYLLGDESHY KIFDLKNKEF TFTVDDSNLE CGLNGALYFV AMDEDGGASR FTLAKPGAKY



GTGYCDAQCP HDIKFINGEA NVQDWKPSDN DDNAGTGHYG ACCTEMDIWE ANKYATAYTP HICTENGEYR CEGKSCGDSS DDRYGGVCDK



DGCDFNSWRL GNQSFWGPGL IIDTGKPVTV VTQFVTKDGT DSGALSEIRR KYVQGGKTIE NTVVKISGID EVDSITDEFC NQQKQAFGDT



NDFEKKGGLS GLGKAFDYGV VLVLSLWDDH DVNMLWLDSV YPTNPAGKAG ADRGPCATSS GDPKEVEDKY ASASVTFSDI KFGPIDSTY


 


SEQ ID NO: 133
MLVFGIVSFV YSIGVGTNTA ETHPKLTWKN GGSTTNGEVT VDSNWRWTHT KGSTKNCYDG NLWSKDLCPD AATCGKNCVL EGADYSGTYG



VTSSGDALTL KFVTHGSYST NVGSRLYLLK DEKTYQMFNL NGKEFTFTVD VSQLPCGLNG ALYFVCMDQD GGMSRYPDNQ AGAKYGTGYC



DAQCPTDLKF INGLPNSDGW KPQSNDKNSG NGKYGSCCSE MDIWEANSLA TAVTPHVCDQ VGQTRCEGRA CGENGGGDRF GSICDPDGCD



FNSWRMGNKT FWGPGLIIDT KKPVTVVTQF IGSPVTEIKR EYVQGGKVIE NSYTNIEGMD KFNSISDKFC TAQKKAFGDN DSFTKHGGFS



KLGQSFTKGQ VLVLSLWDDH TVNMLWLDSV YPTNSKKLGS DRGPCPTSSG VPADVESKNA DSSVKYSDIR FGSIDSTYK


 


SEQ ID NO: 134
MLSFVFLLGF GVSLEIGTQQ SENHPTLSWQ QCTSSGSCTS QSGSIVLDSN WRWVHDSGTT NCYDGNEWSS DLCPDPETCS KNCYLDGADY



SGTYGITSNG SSLKLGFVTE GSYSTNIGSR VYLKKDTNTY QIFKLKNHEF TFTVDVSNLP CGLNGALYFV EMEADGGKGK YPLAKPGAQY



GMGYCDAQCP HDMKFINGNA NVLDWKPQET DENSGNGRYG TCCTEMDIWE ANSQATAYTP HICTKDGQYQ CEGTECGDSD ANQRYNGVCD



KDGCDFNSYR LGNKTFFGPG LIVDSKKPVT VVTQFITSNG QDSGDLTEIR RIYVQGGKTI QNSFTNIAGL TSVDSITEAF CDESKDLFGD



TNDFKAKGGF TAMGKSLDTG VVLVLSLWDD HSVNMLWLDS TYPTDAAAGA LGTQRGPCAT SSGAPSDVES QSPDASVTFS DIKFGPLDST Y


 


SEQ ID NO: 135
MLTLVVYLLS LVVSLEIGTQ QSESHPALTW QREGSSASGS IVLDSNWRWV HDSGTTNCYD GNEWSTDLCP SSDTCTQKCY IEGADYSGTY



GITTSGSKLT LKFVTKGSYS TNIGSRVYLL KDENTYETFK LKNKEFTFTV DDSKLDCGLN GALYFVAMDA DGGKQKYSSF KPGAKYGMGY



CDAQCPHDMK FISGKANVED WKPQDNDENS GNGKLGTCCS EMDIWEGNAK SQAYTVHACT KSGQYECTGT DCGDSDSRYQ GTCDKDGCDY



ASYRWGDHSF YGEGKTVDTK QPITVVTQFI GDPLTEIRRL YIQGGKVINN SKTQNLASVY DSITDAFCDA TKAASGDTND FKAKGAMAGF



SKNLDTPQVL VLSLWDDHTA NMLWLDSTYP TDSRDATAER GPCATSSGVP KDVESNQADA SVVFSDIKFG AINSTYSYN


 


SEQ ID NO: 136
MFGFLLSLFA LQFALEIGTQ TSESHPSITW ELNGARQSGQ IVIDSNWRWL HDSGTTNCYD GNTWSSDLCP DPEKCSQNCY LEGADYSGTY



GISASGSQLT LGFVTKGSYS TNIGSRVYLL KDENTYQMFK LKNKEFTFTV DVSNLPCGLN GALYFVAMPS DGGKAKYPLA KPGAKYGMGY



CDAQCPHDMK FINGEANVLD WKPQSNDENA GTGRYGTCCT EMDIWEANSQ ATAYTVHACS KNARCEGTEC GDDSASQRYN GICDKDGCDF



NSWRWGNKTF FGPGLTVDSS KPVTVVTQFI GDPLTEIRRI WVQGGKVIQN SFTNVSGITS VDSITNTFCD ESKVATGDTN DFKAKGGMSG FSKALDTEVV LVLSLWDDHT



ANMLWLDSTY PSNSTAIGAT RGPCATSSGD PKNVESASAN ASVKFSDIKF GAFDSTY


 


SEQ ID NO: 137
MLALVYFLLS LVVSLEIGTQ QSEDHPKLTW QNGSSSVSGS IVLDSNWRWV HDSGTTNCYD GNLWSTDLCP SSDTCTSKCY IEGADYSGTY



GITSSGSKVT LKFVTKGSYS TNIGSRIYLL KDENTYETFK LKNKEFTFTV DDSQLNCGLN GALYFVAMDA DGGKAKYSSF KPGAKYGMGY



CDAQCPHDMK FISGKANVDD WKPQDNDENS GNGKLGTCCS EMDIWEGNAK SQAYTVHACT KSGQYECTGQ QCGDTDSGDR FKGTCDKDGC



DYASWRWGDQ SFYGEGKTVD TKQPVTVVTQ FIGDPLTEIR RLYVQGGKTI NNSKTSNLAD TYDSITDKFC DATKEASGDT NDFKAKGAMS



GFSTNLNTAQ VLVLSLWDDH TANMLWLDST YPTDSTKTGA SRGPCAVTSG VPKDVESQYG SAQVVYSDIK FGAINSTY


 


SEQ ID NO: 138
MLALVYFLLS FVVSLEIGTQ QSEDHPKLTW QNGSSSVSGS IVLDSNWRWV HDSGTTNCYD GNLWSTDLCG SSDTCSSKCY IEGADYSGTY



GISASGSKLT LKFVTKGSYS TNIGSRVYLL KDENTYETFK LKGKEFTFTV DDSKLDCGLN GALYFVAMDA DGGKAKYSSF KPGAKYGMGY



CDAQCPHDMK FISGKANVDD WKPQDNDENS GNGKLGTCCS EMDIWEGNAK SQAYTVHACT KSGQYECTGQ QCGDTDSGDR FKGTCDKDGC



DYASWRWGDQ SFYGEGKTID TKQPVTVVTQ FIGDPLTEIR RVYVQGGKVI NNSKTSNLAN VYDSITDKFC DDTKDATGDT NDFKAKGAMS



GFSTNLNTAQ VLVMSLWDDH TANMLWLDST YPTDSTKTGA SRGPCAVLSG VPKNVESQHG DATVIYSDIK FGAINSTFSY N


 


SEQ ID NO: 139
MFLALFVLGK SLGIATNQAE NHPKLTWTRY QSKGSGQTVN GEVVLDSNWR WTHHSGTNCY DGNTWSTSLC PDPQTCSSNC DLDGADYPGT



YGISSSGNSL KLGFVTHGSY STNIGSRVYL LRDSKNYEMF KLKNKEFTFT VDDSKLPCGL NGALYFVAME EDGGVAKNSI NKAGAQYGTG



YCDAQCPHDM KFINGEANVL DWKPQSNDEN SGNGRYGACC IEMDIWEANS MATAYTPHVC TVTGIHRCEG TECGDTDANQ RYNGICDKDG



CDFNSYRMGD KSFFGVGKTV DSSKPVTVVT QFVTSNGQDG GTLSEIKRKY VQGGKVIENS KVNIAGITAV NSITDTFCNE QKKAFGDNND



FEKKGGLGAL SKQLDLGMVL VLSLWDDHSV NMLWLDSTYP TDAAAGALGT ERGACATSSG KPSDVESQSP DASVTFSDIK FGPIDSTY


 


SEQ ID NO: 140
MLLCLLSIAN SLGVGTNTAE NHPKLSWKNG GSSVSGSVTV DANWRWTHIK GETKNCYDGN LWSDKYCPDA ATCGKNCVIE GADYQGTYGV



SSSGDGLTLT FVTHGQYSTN VGSRLYLMKD EKTYQMFNLN GKEFTFTVDV SNLPCGLNGA LYFVQMDSDG GMAKYPDNQA GAKYGTGYCD



AQCPTDLKFI NGIPNSDGWK PQKNDKNSGN GKYGSCCSEM DIWEANSQAT AYTPHVCDKL EQTRCSGSSC GHTGGGERFS SSCDPDGCDF



NSWRMGNKTF WGPGLIVDTK KPVQVVTQFV GSGNSCTEIK RKYVQGGKVI DNSMSNIAGM SKQYNSVSDD FCQAQKKAFG DNDSFTKHGG FRQLGATLGK GHVLVLSLWD



DHDVNMLWLD SVYPTNSNKP GSDRGPCKTS SGIPADVESQ AASSSVKYSD IRFGAIDSTY K


 


SEQ ID NO: 141
MLCIGLISFV YSLGVGTNTA ETHPKLTWKN GGQTVNGEVT VDSNWRWTHT KGSTKNCYDG NLWSKDLCPD AATCGKNCVL EGADYSGTYG



VTSSGNALTL KFVTHGSYST NVGSRLYLMK DEKTYQMFNL NGKEFTFTVD VSNLPCGLNG ALYHVNMDED GGTKRYPDNE AGAKYGTGYC



DAQCPTDLKF INGIPNSDGW KPQSNDKNSG NGKYGSCCSE MDIWEANSIC SAVTPHVCDT LQQTRCQGTA CGENGGGSRF GSSCDPDGCD



FNSWRMGNKT FYGPGLIVDT KSKFTVVTQF VGSPVTEIKR KYVQNGKVIE NSFSNIEGMD KFNSISDKFC TAQKKAFGDT DSFTKHGGFK



QLGSALAKGM VLVLSLWDDH TVNMLWLDSV YPTNSKKAGS DRGPCPTTSG VPADVESKSA NANVIYSDIR FGAIDSTYK


 


SEQ ID NO: 142
MLLCLLGIAS SLDAGTNTAE NHPQLSWKNG GSSVSGSVTV DANWRWTHIK GETKNCYDGN LWSDKYCPDA ATCGQNCVIE GADYQGTYGV



SASGNALTLT FVTHGQYSTN VGSRLYLLKD EKTYQIFNLI GKEFTFTVDV SNLPCGLNGA LYFVQMDADG GTAKYSDNKA GAKYGTGYCD



AQCPTDLKFI NGIPNSDGWK PQKNDKNSGN GRYGSCCSEM DVWEANSLAT AYTPHVCDKL EQVRCDGRAC GQNGGGDRFS SSCDPDGCDF



NSWRLGNKTF WGPGLIVDTK QPVQVVTQWV GSGTSVTEIK RKYVQGGKVI DNSFTKLDSL TKQYNSVSDE FCVAQKKAFG DNDSFTKHGG



FRQLGATLAK GHVLVLSLWD DHDVNMLWLD SVYPTNSNKP GADRGPCKTS SGVPADVESQ AASSSVKYSD IRFGAIDSTY K


 


SEQ ID NO: 143
MLGIGFVCIV YSLGVGTNTA ENHPKLTWKN SGSTTNGEVT VDSNWRWTHT KGTTKNCYDG NLWSKDLCPD AATCGKNCVL EGADYSGTYG



VTSSGDALTL KFVTHGSYST NVGSRLYLLK DEKTYQIFNL NGKEFTFTVD VSNLPCGLNG ALYFVNMDAD GGTGRYPDNQ AGAKYGTGYC



DAQCPTDLKF INGIPNSDGW KPQSNDKNSG NGKYGSCCSE MDIWEANSLA TAVTPHVCDQ VGQTRCEGRA CGENGGGDRF GSSCDPDGCD



FNSWRLGNKT FWGPGLIVDT KKPVTVVTQF VGSPVTEIKR KYVQGGKVIE NSYTNIEGLD KFNSISDKFC TAQKKAFGDN DSFIKHGGFR



QLGQSFTKGQ VLVLSLWDDH TVNMLWLDSV YPTNSKKPGA DRGPCPTSSG VPADVESKNA GSSVKYSDIR FGSIDSTYK


 


SEQ ID NO: 144
MATLVGILVS LFALEVALEI GTQTSESHPS LSWELNGQRQ TGSIVIDSNW RWLHDSGTTN CYDGNEWSSD LCPDPEKCSQ NCYLEGADYS



GTYGISSSGN SLQLGFVTKG SYSTNIGSRV YLLKDENTYA TFKLKNKEFT FTADVSNLPC GLNGALYFVA MPADGGKSKY PLAKPGAKYG



MGYCDAQCPH DMKFINGEAN ILDWKPSSND ENAGAGRYGT CCTEMDIWEA NSQATAYTVH ACSKNARCEG TECGDDDGRY NGICDKDGCD



FNSWRWGNKT FFGPNLIVDS SKPVTVVTQF IGDPLTEIRR IYVQGGKVIQ NSFTNISGVA SVDSITDAFC NENKVATGDT NDFKAKGGMS GFSKALDTEV VLVLSLWDDH



TANMLWLDST YPTDSSALGA SRGPCAITSG EPKDVESASA NASVKFSDIK FGAIDSTY


 


SEQ ID NO: 145
MLTLVYFLLS LVVSLEIGTQ QSESHPQLSW QNGSSSVSGS IVLDSNWRWV HDSGTTNCYD GNLWSTDLCP SSDTCTSKCY IEGADYSGTY



GITSSGSKLT LKFVTKGSYS TNIGSRVYLL KDENTYETFK LKNKEFTFTV DDSKLDCGLN GALYFVAMDA DGGKAKYSSF KPGAKYGMGY



CDAQCPHDMK FISGKANVDD WKPQDNDENS GNGKLGTCCS EMDIWEGNAK SQAYTVHACT KSGQYECTGQ QCGDTDSGDR FKGTCDKDGC



DYASWRWGDQ SFYGEGKTVD TKQPLTVVTQ FVGDPLTEIR RVYVQGGKTI NNSKTSNLAD TYDSITDKFC DATKEASGDT NDFKAKGAMS



GFSTNLNTAQ VLVMSLWDDH TANMLWLDST YPTDSTKTGA SRGPCAVSSG VPKDVESQHG DATVIYSDIK FGAINSTFKW N


 


SEQ ID NO: 146
MLSLVSIFLV GLGFSLGVGT QQSESHPSLS WQNCSAKGSC QSVSGSIVLD SNWRWLHDSG TTNCYDGNEW STDLCPDAST CDKNCYIEGA



DYSGTYGITS SGAQLKLGFV TKGSYSTNIG SRVYLLRDES HYQLFKLKNH EFTFTVDDSQ LPCGLNGALY FVEMAEDGGA KPGAQYGMGY



CDAQCPHDMK FITGEANVKD WKPQETDENA GNGHYGACCT EMDIWEANSQ ATAYTPHICS KTGIYRCEGT ECGDNDANQR YNGVCDKDGC



DFNSYRLGNK TFWGPGLTVD SNKAMIVVTQ FTTSNNQDSG ELSEIRRIYV QGGKTIQNSD TNVQGITTTN KITQAFCDET KVTFGDTNDF



KAKGGFSGLS KSLESGAVLV LSLWDDHSVN MLWLDSTYPT DSAGKPGADR GPCAITSGDP KDVESQSPNA SVTFSDIKFG PIDSTY


 


SEQ ID NO: 147
MILALLVLGK SLGIATNQAE THPKLTWTRY QSKGSGSTVN GEIVLDSNWR WTHHSGTNCY DGNTWSTSLC PDPTTCSNNC DLDGADYPGT



YGISTSGNSL KLGFVTHGSY STNIGSRVYL LKDTKSYEMF KLKNKEFTFT VDDSKLPCGL NGALYFVAMD EDGGVSKNSI NKAGAQYGTG



YCDAQCPHDM KFINGEANVL DWKPQSNDEN SGNGRYGACC TEMDIWEANS MATAYTPHVC TVTGLRRCEG TECGDTDNDQ RYNGICDKDG



CDFNSYRLGD KSFFGVGKTV DSSKPVTVVT QFVTSNGQDS GTLSEIRRKY VQGGKVIENS KVNVAGITAG NSVTDTFCNE QKKAFGDNND FEKKGGFGAL SKQLVAGMVL



VLSLWDDHSV NMLWLDSTYP TNAAAGALGT ERGACATSSG KPSDVESQSP DATVTFSDIK FGPIDSTY


 


SEQ ID NO: 148
MLCVGLFGLV YSIGVGTNTQ ETHPKLSWKQ CSSGGSCTTQ QGSVVIDSNW RWTHSTKDLT NCYDGNLWDS TLCPDGTTCS KNCVLEGADY



SGTYGITSSG DSLTLKFVTH GSYSTNVGSR LYLLKDDNNY QIFNLAGKEF TFTVDVSNLP CGLNGALYFV EMDQDGGKGK HKENEAGAKY



GTGYCDAQCP TDLKFIDGIA NSDGWKPQDN DENSGNGKYG SCCSEMDIWE ANSLATAYTP HVCDTKGQKR CQGTACGENG GGDRFGSECD



PDGCDFNSWR QGNKSFWGPG LIIDTKKSVQ VVTQFIGSGS SVTEIRRKYV QNGKVIENSY STISGTEKYN SISDDYCNAQ KKAFGDTNSF



ENHGGFKRFS QHIQDMVLVL SLWDDHTVNM LWLDSVYPTN SNKPGADRGP CETSSGVPAD VESKSASASV KYSDIRFGPI DSTYK


 


SEQ ID NO: 149
MLLCLWSIAY SLGVGTNTAE NHPKLSWKNG GSSVSGSVTV DANWRWTHIK GETKNCYDGN LWSDKYCPDA ATCGKNCVIE GADYQGTYGV



SASGDGLTLT FVTHGQYSTN VGSRLYLMKD EKTYQIFNLN GKEFTFTVDV SNLPCGLNGA LYFVQMDSDG GMAKYPDNQA GAKYGTGYCD



AQCPTDLKFI NGIPNSDGWK PQKNDKNSGN GKYGSCCSEM DIWEANSQAT AYTPHVCDKL EQTRCSGSAC GHTGGGERFS SSCDPDGCDF



NSWRMGNKTF WGPGLIVDTK KPVQVVTQFV GSGNSCTEIK RKYVQGGKVI DNSMSNIAGM TKQYNSVSDD FCQAQKKAFG DNDSFTKHGG



FRQLGATLGK GHVLVLSLWD DHDVNMLWLD SVYPTNSNKP GSDRGPCKTS SGIPADVESQ AASSSVKYSD IRFGAIDSTY K


 


SEQ ID NO: 299
QSACTLQSET HPPLTWQKCS SGGTCTQQTG SVVIDANWRW THATNSSTNC YDGNTWSSTL CPDNETCAKN CCLDGAAYAS TYGVTTSGNS



LSIGFVTQSA QKNVGARLYL MASDTTYQEF TLLGNEFSFD VDVSQLPCGL NGALYFVSMD ADGGVSKYPT NTAGAKYGTG YCDSQCPRDL



KFINGQANVE GWEPSSNNAN TGIGGHGSCC SEMDIWEANS ISEALTPHPC TTVGQEICEG DGCGGTYSDN AYGGTCDPDG CDWNPYRLGN



TSFYGPGSSF TLDTTKKLTV VTQFETSGAI NRYYVQNGVT FQQPNAELGS YSGNELNDDY CTAEEAEFGG SSFSDKGGLT QFKKATSGGM



VLVMSLWDDY YANMLWLDST YPTNETSSTP GAVRGSCSTS SGVPAQVESQ SPNAKVTFSN IKFGPIGSTG NPSGGNPPGG NPPGTTTTRR



PATTTGSSPG PTQSHYGQCG GIGYSGPTVC ASGTTCQVLN PYYSQCL


 


SEQ ID NO: 300
QSACTLQSET HPPLTWQKCS SGGTCTQQTG SVVIDANWRW THATNSSTNC YDGNTWSSTL CPDNETCAKN CCLDGAAYAS TYGVTTSGNS



LSIGFVTQSA QKNVGARLYL MASDTTYQEF TLLGNEFSFD VDVSQLPCGL NGALYFVSMD ADGGVSKYPT NTAGAKYGTG YCDSQCPRDL



KFINGQANVE GWEPSSNNAN TGIGGHGSCC SEMDIWEANS ISEALTPHPC TTVGQEICEG DGCGGTYSDN RYGGTCDPDG CDWNPYRLGN



TSFYGPGSSF TLDTTKKLTV VTQFETSGAI NRYYVQNGVT FQQPNAELGS YSGNELNDDY CTAEEAEFGG SSFSDKGGLT QFKKATSGGM



VLVMSLWDDY YANMLWLDST YPTNETSSTP GAVAGSCSTS SGVPAQVESQ SPNAKVTFSN IKFGPIGSTG NPSGGNPPGG NPPGTTTTRR



PATTTGSSPG PTQSHYGQCG GIGYSGPTVC ASGTTCQVLN PYYSQCL


 


SEQ ID NO: 301
MSALNSFNMY KSALILGSLL ATAGAQQIGT YTAETHPSLS WSTCKSGGSC TTNSGAITLD ANWRWVHGVN TSTNCYTGNT WNTAICDTDA



SCAQDCALDG ADYSGTYGIT TSGNSLRLNF VTGSNVGSRT YLMADNTHYQ IFDLLNQEFT FTVDVSHLPC GLNGALYFVT MDADGGVSKY



PNNKAGAQYG VGYCDSQCPR DLKFIAGQAN VEGWTPSSNN ANTGLGNHGA CCAELDIWEA NSISEALTPH PCDTPGLSVC TTDACGGTYS



SDKYAGTCDP DGCDFNPYRL GVTDFYGSGK TVDTTKPITV VTQFVTDDGT STGTLSEIRR YYVQNGVVIP QPSSKISGVS GNVINSDFCD



AEISTFGETA SFSKHGGLAK MGAGMEAGMV LVMSLWDDYS VNMLWLDSTY PTNATGTPGA AKGSCPTTSG DPKTVESQSG SSYVTFSDIR VGPFNSTFSG GSSTGGSSTT



TASGTTTTKA SSTSTSSTST GTGVAAHWGQ CGGQGWTGPT TCASGTTCTV VNPYYSQCL


 


SEQ ID NO: 302
QQIGTYTAET HPSLSWSTCK SGGSCTTNSG AITLDANWRW VHGVNTSTNC YTGNTWNTAI CDTDASCAQD CALDGADYSG TYGITTSGNS



LRLNFVTGSN VGSRTYLMAD NTHYQIFDLL NQEFTFTVDV SHLPCGLNGA LYFVTMDADG GVSKYPNNKA GAQYGVGYCD SQCPRDLKFI



AGQANVEGWT PSSNNANTGL GNHGACCAEL DIWEANSISE ALTPHPCDTP GLSVCTTDAC GGTYSSDKYA GTCDPDGCDF NPYRLGVTDF



YGSGKTVDTT KPITVVTQFV TDDGTSTGTL SEIRRYYVQN GVVIPQPSSK ISGVSGNVIN SDFCDAEIST FGETASFSKH GGLAKMGAGM



EAGMVLVMSL WDDYSVNMLW LDSTYPTNAT GTPGAAKGSC PTTSGDPKTV ESQSGSSYVT FSDIRVGPFN STFSGGSSTG GSSTTTASGT



TTTKASSTST SSTSTGTGVA AHWGQCGGQG WTGPTTCASG TTCTVVNPYY SQCL

## Claims

1. A polypeptide comprising a variant cellobiohydrolase I (CBH I) catalytic domain as compared to a reference CBH I catalytic domain, wherein the variant CBH I catalytic domain comprises an amino acid sequence having at least 90% sequence identity to amino acids 26-455 of SEQ ID NO:1, the polypeptide comprising:
(a) a substitution at the amino acid position corresponding to R268 of T. reesei CBH I of SEQ ID NO:2(R268 substitution);
(b) a substitution at the amino acid position corresponding to R411 of T. reesei CBH I of SEQ ID NO: 2 (R411 substitution); or
(c) both an R268 substitution and an R411 substitution,
wherein substitution (a), (b) or (c) decreases product inhibition as compared to the reference CBH I catalytic domain.

2. The polypeptide of claim 1, which comprises an R268 substitution.

3. The polypeptide of claim 2, wherein the R268 substituent is a lysine.

4. The polypeptide of claim 2, wherein the R268 substituent is an alanine.

5. The polypeptide of claim 1, which comprises an R411 substitution.

6. The polypeptide of claim 5, wherein the R411 substituent is a lysine.

7. The polypeptide of claim 5, wherein the R411 substituent is an alanine.

8. The polypeptide of claim 1, wherein the variant CBH I catalytic domain comprises an amino acid sequence having at least 95% sequence identity to amino acids 26-455 of SEQ ID NO:1.

9. The polypeptide of claim 8, wherein, other than said R268 and/or R411 substitutions, the variant CBH I catalytic domain comprises the sequence of amino acids 26-455 of SEQ ID NO:1.

10. The polypeptide of claim 1, wherein the variant CBH I catalytic domain comprises one of the following amino acid substitutions or pairs of amino acid substitutions as compared to a protein of SEQ ID NO:1:
(a) R273K and R422K;
(b) R273K and R422A;
(c) R273A and R422K;
(d) R273A and R422A;
(e) R273A;
(f) R273K;
(g) R422A; and
(h) R422K.

11. The polypeptide of claim 1, wherein the variant CBH I catalytic domain comprises the amino acid substitutions R273K and R422K as compared to a protein of SEQ ID NO:1.

12. The polypeptide of claim 1, wherein the variant CBH I catalytic domain does not comprise both R273K and R422K substitutions as compared to a protein of SEQ ID NO:1.

13. The polypeptide of claim 1 in which the variant CBH I catalytic domain is linked to a cellulose binding domain.

14. The polypeptide of claim 13 in which the catalytic domain is linked to a cellulose binding domain via a linker.

15. The polypeptide of claim 13 in which the cellulose binding domain is C-terminal to the catalytic domain.

16. The polypeptide of claim 13 in which the cellulose binding domain is N-terminal to the catalytic domain.

17. The polypeptide of claim 1 which further comprises a signal sequence.

18. The polypeptide of claim 1 towards which cellobiose has an IC50 that is at least 2-fold the IC50 of a reference CBH I lacking said R268 substitution and/or R411 substitution.

19. The polypeptide of claim 1 which CBH I activity that is at least 50% the CBH I activity of a reference CBH I lacking said R268 substitution and/or R411 substitution.

20. A composition comprising a polypeptide according to claim 1.

21. The composition of claim 20 in which said polypeptide represents at least 1% of all polypeptides in said composition.

22. The composition of claim 21 in which said polypeptide represents at least 5% of all polypeptide in said composition.

23. The composition of claim 22 in which said polypeptide represents at least 25% of all polypeptide in said composition.

24. The composition of claim 20 which is a whole cellulase.

25. The composition of claim 24, wherein the whole cellulase is produced by a host cell that recombinantly expresses said polypeptide.

26. The composition of claim 20 which is filamentous fungal whole cellulase.

27. A fermentation broth comprising a polypeptide according to claim 1.

28. The fermentation broth of claim 27, which is a filamentous fungal fermentation broth.

29. The fermentation broth of claim 27, which is a cell-free fermentation broth.

30. A method for saccharifying biomass, comprising: treating biomass with a composition according to claim 20.

31. The method of claim 30, further comprising recovering monosaccharides.

32. A nucleic acid comprising a nucleotide sequence encoding the polypeptide of claim 1.

33. A vector comprising the nucleic acid of claim 32.

34. The vector of claim 33 which further comprises an origin of replication.

35. The vector of claim 33 which further comprises a promoter sequence operably linked to said nucleotide sequence.

36. The vector of claim 35, wherein the promoter sequence is operable in yeast.

37. The vector of claim 35, wherein the promoter sequence is operable in filamentous fungi.

38. A recombinant cell engineered to express the nucleic acid of claim 32.

39. The recombinant cell of claim 38 which is a eukaryotic cell.

40. The recombinant cell of claim 39 which is a filamentous fungal cell.

41. The recombinant cell of claim 40, wherein the filamentous fungal cell is of the genus Aspergillus, Penicillium, Rhizopus, Chrysosporium, Myceliophthora, Trichoderma, Humicola, Acremonium or Fusarium.

42. The recombinant cell of claim 40, wherein the filamentous fungal cell is of the species Aspergillus niger, Aspergillus oryzae, Trichoderma reesei, Penicillium chrysogenum, Myceliophthora thermophila, or Rhizopus oryzae.

43. The recombinant cell of claim 39 which is a yeast cell.

44. The recombinant cell of claim 43 which is a yeast cell of the genus Saccharomyces, Kluyveromyces, Candida, Pichia, Schizosaccharomyces, Hansenula, Klockera, Schwanniomyces or Yarrowia.

45. The recombinant cell of claim 44, wherein the yeast cell is of the species S. cerevisiae, S. bulderi, S. barnetti, S. exiguus, S uvarum, S. diastaticus, K lactis, K. marxianus or K. fragilis.

46. The recombinant cell of claim 45, which is a S. cerevisiae cell.

47. A host cell transformed with the vector of claim 33.

48. The host cell of claim 47 which is a prokaryotic cell.

49. The host cell of claim 48 which is a bacterial cell.

50. The host cell of claim 47 which is a eukaryotic cell.

51. A method of producing a polypeptide according to claim 1, comprising culturing the recombinant cell engineered to express said polypeptide under conditions in which the polypeptide is expressed.

52. The method of claim 51, wherein the polypeptide comprises a signal sequence and wherein the recombinant cell is cultured under conditions in which the polypeptide is secreted from the recombinant cell.

53. The method of claim 52, further comprising recovering the polypeptide from the cell culture.

54. The method of claim 53, wherein recovering the polypeptide comprises a step of centrifuging away cells and/or cellular debris.

55. The method of claim 53, wherein recovering the polypeptide comprises a step of filtering away cells and/or cellular debris.

56. A method for generating a product tolerant variant CBH I polypeptide, wherein the variant CBH I catalytic domain comprises an amino acid sequence having at least 90% sequence identity to amino acids 26-455 of SEQ ID NO:1 comprising:
(a) modifying the nucleotide sequence of a CBH I-encoding nucleic acid so that the nucleic acid encodes a variant CBH I polypeptide, wherein said variant CBH I polypeptide comprises:
(i) an R273 substitution of SEQ ID NO: 1;
(ii) an R422 substitution of SEQ ID NO: 1; or
(iii) both an R273 substitution and an R422 substitution; and

(b) expressing said variant CBH I polypeptide, thereby generating a product tolerant variant CBH I polypeptide.

57. A method for producing ethanol, comprising:
(a) treating biomass with a fermentation broth according to claim 27, thereby producing monosaccharides; and
(b) culturing a fermenting microorganism in the presence of the monosaccharides produced in step (a) under fermentation conditions, thereby producing ethanol.